LOCUS       DAZXUK010000001        63469 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZXUK010000001 DAZXUK010000000
VERSION     DAZXUK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63469
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.001"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..795
                     /locus_tag="ACH3L8_000001"
     CDS             <1..795
                     /locus_tag="ACH3L8_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFT4083304.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="ACH3L8_000002"
     CDS             806..1897
                     /locus_tag="ACH3L8_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4083305.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACH3L8_000003"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACH3L8_000003"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFT4083306.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="ACH3L8_000004"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="ACH3L8_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFT4083307.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="ACH3L8_000005"
     CDS             4859..5254
                     /locus_tag="ACH3L8_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFT4083308.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="ACH3L8_000006"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="ACH3L8_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFT4083309.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="dolP"
                     /locus_tag="ACH3L8_000007"
     CDS             5874..6449
                     /gene="dolP"
                     /locus_tag="ACH3L8_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFT4083310.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="ACH3L8_000008"
     CDS             complement(6563..7603)
                     /locus_tag="ACH3L8_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFT4083311.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="ACH3L8_000009"
     CDS             complement(7676..8311)
                     /locus_tag="ACH3L8_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFT4083312.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="ACH3L8_000010"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="ACH3L8_000010"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFT4083313.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="ACH3L8_000011"
     CDS             complement(8937..9380)
                     /locus_tag="ACH3L8_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFT4083314.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACH3L8_000012"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACH3L8_000012"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFT4083315.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="ACH3L8_000013"
     CDS             complement(9720..10223)
                     /locus_tag="ACH3L8_000013"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFT4083316.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="ACH3L8_000014"
     CDS             complement(10217..10741)
                     /locus_tag="ACH3L8_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFT4083317.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="ACH3L8_000015"
     CDS             10950..11945
                     /locus_tag="ACH3L8_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFT4083318.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="ACH3L8_000016"
     CDS             11954..12832
                     /locus_tag="ACH3L8_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFT4083319.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="ACH3L8_000017"
     CDS             12913..13920
                     /locus_tag="ACH3L8_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFT4083320.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACH3L8_000018"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACH3L8_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFT4083321.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACH3L8_000019"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACH3L8_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFT4083322.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACH3L8_000020"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACH3L8_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFT4083323.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACH3L8_000021"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACH3L8_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFT4083324.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACH3L8_000022"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACH3L8_000022"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFT4083325.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACH3L8_000023"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACH3L8_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFT4083326.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACH3L8_000024"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACH3L8_000024"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFT4083327.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACH3L8_000025"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACH3L8_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFT4083328.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACH3L8_000026"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACH3L8_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFT4083329.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACH3L8_000027"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACH3L8_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFT4083330.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACH3L8_000028"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACH3L8_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFT4083331.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="ACH3L8_000029"
     tRNA            complement(27654..27730)
                     /locus_tag="ACH3L8_000029"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="ACH3L8_000030"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="ACH3L8_000030"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFT4083332.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31055)
                     /locus_tag="ACH3L8_000031"
                     /pseudo
     CDS             complement(29429..31055)
                     /locus_tag="ACH3L8_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(31514..31600)
                     /locus_tag="ACH3L8_000032"
     tRNA            complement(31514..31600)
                     /locus_tag="ACH3L8_000032"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACH3L8_000033"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACH3L8_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFT4083333.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACH3L8_000034"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACH3L8_000034"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFT4083334.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACH3L8_000035"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACH3L8_000035"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFT4083335.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACH3L8_000036"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACH3L8_000036"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFT4083336.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACH3L8_000037"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACH3L8_000037"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFT4083337.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="ACH3L8_000038"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="ACH3L8_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFT4083338.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACH3L8_000039"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACH3L8_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFT4083339.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="ACH3L8_000040"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="ACH3L8_000040"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFT4083340.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            39902..40003
                     /locus_tag="ACH3L8_000041"
                     /pseudo
     CDS             39902..40003
                     /locus_tag="ACH3L8_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACH3L8_000042"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACH3L8_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFT4083341.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="ACH3L8_000043"
     CDS             complement(41212..42177)
                     /locus_tag="ACH3L8_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFT4083342.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACH3L8_000044"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACH3L8_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFT4083343.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACH3L8_000045"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACH3L8_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFT4083344.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="ACH3L8_000046"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="ACH3L8_000046"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFT4083345.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="ACH3L8_000047"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="ACH3L8_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFT4083346.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACH3L8_000048"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACH3L8_000048"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFT4083347.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACH3L8_000049"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACH3L8_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFT4083348.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACH3L8_000050"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACH3L8_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFT4083349.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACH3L8_000051"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACH3L8_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFT4083350.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACH3L8_000052"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACH3L8_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFT4083351.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACH3L8_000053"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACH3L8_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFT4083352.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACH3L8_000054"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACH3L8_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFT4083353.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="ACH3L8_000055"
     CDS             49715..50680
                     /locus_tag="ACH3L8_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFT4083354.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="ACH3L8_000056"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="ACH3L8_000056"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFT4083355.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="ACH3L8_000057"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="ACH3L8_000057"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFT4083356.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="ACH3L8_000058"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="ACH3L8_000058"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFT4083357.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="ACH3L8_000059"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="ACH3L8_000059"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFT4083358.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="ACH3L8_000060"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="ACH3L8_000060"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFT4083359.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="ACH3L8_000061"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="ACH3L8_000061"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFT4083360.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="ACH3L8_000062"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="ACH3L8_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFT4083361.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="ACH3L8_000063"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="ACH3L8_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFT4083362.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="ACH3L8_000064"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="ACH3L8_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFT4083363.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="ACH3L8_000065"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="ACH3L8_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFT4083364.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="ACH3L8_000066"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="ACH3L8_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFT4083365.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACH3L8_000067"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACH3L8_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACH3L8_000068"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACH3L8_000068"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFT4083366.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACH3L8_000069"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACH3L8_000069"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFT4083367.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..>63469)
                     /locus_tag="ACH3L8_000070"
     CDS             complement(62547..>63469)
                     /locus_tag="ACH3L8_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFT4083368.1"
                     /translation="QKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGGCTF
                     CNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQQAV
                     SQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRGHDF
                     ACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHIVKG
                     SIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWCENR
                     WTGMVELDRYLNEHGVQGSALGRPWLPPTE"
BASE COUNT        15599 a        17229 c        15566 g        15075 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaggt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttat
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgta
//
LOCUS       DAZXUK010000002        56471 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZXUK010000002 DAZXUK010000000
VERSION     DAZXUK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56471
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.002"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="ACH3L8_000071"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="ACH3L8_000071"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFT4083369.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2001)
                     /gene="pcnB"
                     /locus_tag="ACH3L8_000072"
     CDS             complement(604..2001)
                     /gene="pcnB"
                     /locus_tag="ACH3L8_000072"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFT4083370.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTT"
     gene            complement(2061..2957)
                     /gene="gluQRS"
                     /locus_tag="ACH3L8_000073"
     CDS             complement(2061..2957)
                     /gene="gluQRS"
                     /locus_tag="ACH3L8_000073"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFT4083371.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="ACH3L8_000074"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="ACH3L8_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFT4083372.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="ACH3L8_000075"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="ACH3L8_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFT4083373.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="ACH3L8_000076"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="ACH3L8_000076"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFT4083374.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="ACH3L8_000077"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="ACH3L8_000077"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFT4083375.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="ACH3L8_000078"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="ACH3L8_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFT4083376.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="ACH3L8_000079"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="ACH3L8_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFT4083377.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTNKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="ACH3L8_000080"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="ACH3L8_000080"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFT4083378.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="ACH3L8_000081"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="ACH3L8_000081"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFT4083379.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="ACH3L8_000082"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="ACH3L8_000082"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFT4083380.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="ACH3L8_000083"
     CDS             complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="ACH3L8_000083"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFT4083381.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17800..17847
                     /gene="yadX"
                     /locus_tag="ACH3L8_000084"
     CDS             17800..17847
                     /gene="yadX"
                     /locus_tag="ACH3L8_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFT4083382.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17859..19280
                     /gene="clcA"
                     /locus_tag="ACH3L8_000085"
     CDS             17859..19280
                     /gene="clcA"
                     /locus_tag="ACH3L8_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFT4083383.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19304..19369
                     /gene="yadW"
                     /locus_tag="ACH3L8_000086"
     CDS             19304..19369
                     /gene="yadW"
                     /locus_tag="ACH3L8_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFT4083384.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19362..19706
                     /gene="erpA"
                     /locus_tag="ACH3L8_000087"
     CDS             19362..19706
                     /gene="erpA"
                     /locus_tag="ACH3L8_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFT4083385.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19753..20376)
                     /locus_tag="ACH3L8_000088"
     CDS             complement(19753..20376)
                     /locus_tag="ACH3L8_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFT4083386.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="ACH3L8_000089"
     CDS             complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="ACH3L8_000089"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFT4083387.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNLVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="ACH3L8_000090"
     CDS             complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="ACH3L8_000090"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFT4083388.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21989..23506
                     /gene="dgt"
                     /locus_tag="ACH3L8_000091"
     CDS             21989..23506
                     /gene="dgt"
                     /locus_tag="ACH3L8_000091"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFT4083389.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23636..25060
                     /gene="degP"
                     /locus_tag="ACH3L8_000092"
     CDS             23636..25060
                     /gene="degP"
                     /locus_tag="ACH3L8_000092"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFT4083390.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25215..26371
                     /gene="cdaR"
                     /locus_tag="ACH3L8_000093"
                     /pseudo
     CDS             25215..26371
                     /gene="cdaR"
                     /locus_tag="ACH3L8_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26460..26846)
                     /locus_tag="ACH3L8_000094"
     CDS             complement(26460..26846)
                     /locus_tag="ACH3L8_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFT4083391.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="ACH3L8_000095"
     CDS             complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="ACH3L8_000095"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFT4083392.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQATPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="ACH3L8_000096"
     CDS             complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="ACH3L8_000096"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFT4083393.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30749..31543)
                     /gene="map"
                     /locus_tag="ACH3L8_000097"
     CDS             complement(30749..31543)
                     /gene="map"
                     /locus_tag="ACH3L8_000097"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFT4083394.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31911..32636
                     /gene="rpsB"
                     /locus_tag="ACH3L8_000098"
     CDS             31911..32636
                     /gene="rpsB"
                     /locus_tag="ACH3L8_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFT4083395.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32894..33745
                     /gene="tsf"
                     /locus_tag="ACH3L8_000099"
     CDS             32894..33745
                     /gene="tsf"
                     /locus_tag="ACH3L8_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFT4083396.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33892..34617
                     /gene="pyrH"
                     /locus_tag="ACH3L8_000100"
     CDS             33892..34617
                     /gene="pyrH"
                     /locus_tag="ACH3L8_000100"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFT4083397.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34909..35466
                     /gene="frr"
                     /locus_tag="ACH3L8_000101"
     CDS             34909..35466
                     /gene="frr"
                     /locus_tag="ACH3L8_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFT4083398.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35558..36754
                     /gene="ispC"
                     /locus_tag="ACH3L8_000102"
     CDS             35558..36754
                     /gene="ispC"
                     /locus_tag="ACH3L8_000102"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFT4083399.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36943..37701
                     /gene="ispU"
                     /locus_tag="ACH3L8_000103"
                     /gene_synonym="uppS"
     CDS             36943..37701
                     /gene="ispU"
                     /locus_tag="ACH3L8_000103"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFT4083400.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37714..38571
                     /gene="cdsA"
                     /locus_tag="ACH3L8_000104"
     CDS             37714..38571
                     /gene="cdsA"
                     /locus_tag="ACH3L8_000104"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFT4083401.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38583..39935
                     /gene="rseP"
                     /locus_tag="ACH3L8_000105"
     CDS             38583..39935
                     /gene="rseP"
                     /locus_tag="ACH3L8_000105"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFT4083402.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39965..42397
                     /gene="bamA"
                     /locus_tag="ACH3L8_000106"
     CDS             39965..42397
                     /gene="bamA"
                     /locus_tag="ACH3L8_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFT4083403.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42519..43004
                     /gene="skp"
                     /locus_tag="ACH3L8_000107"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42519..43004
                     /gene="skp"
                     /locus_tag="ACH3L8_000107"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFT4083404.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43008..44033
                     /gene="lpxD"
                     /locus_tag="ACH3L8_000108"
     CDS             43008..44033
                     /gene="lpxD"
                     /locus_tag="ACH3L8_000108"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFT4083405.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44138..44593
                     /gene="fabZ"
                     /locus_tag="ACH3L8_000109"
     CDS             44138..44593
                     /gene="fabZ"
                     /locus_tag="ACH3L8_000109"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFT4083406.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44597..45385
                     /gene="lpxA"
                     /locus_tag="ACH3L8_000110"
     CDS             44597..45385
                     /gene="lpxA"
                     /locus_tag="ACH3L8_000110"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFT4083407.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45385..46533
                     /gene="lpxB"
                     /locus_tag="ACH3L8_000111"
     CDS             45385..46533
                     /gene="lpxB"
                     /locus_tag="ACH3L8_000111"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFT4083408.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46530..47126
                     /gene="rnhB"
                     /locus_tag="ACH3L8_000112"
     CDS             46530..47126
                     /gene="rnhB"
                     /locus_tag="ACH3L8_000112"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFT4083409.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47163..50645
                     /gene="dnaE"
                     /locus_tag="ACH3L8_000113"
     CDS             47163..50645
                     /gene="dnaE"
                     /locus_tag="ACH3L8_000113"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFT4083410.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50658..51617
                     /gene="accA"
                     /locus_tag="ACH3L8_000114"
     CDS             50658..51617
                     /gene="accA"
                     /locus_tag="ACH3L8_000114"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFT4083411.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51716..53857
                     /gene="ldcC"
                     /locus_tag="ACH3L8_000115"
     CDS             51716..53857
                     /gene="ldcC"
                     /locus_tag="ACH3L8_000115"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFT4083412.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIAPEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53914..54303
                     /locus_tag="ACH3L8_000116"
     CDS             53914..54303
                     /locus_tag="ACH3L8_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFT4083413.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54368..55666
                     /gene="tilS"
                     /locus_tag="ACH3L8_000117"
     CDS             54368..55666
                     /gene="tilS"
                     /locus_tag="ACH3L8_000117"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFT4083414.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55715..55975)
                     /gene="rof"
                     /locus_tag="ACH3L8_000118"
     CDS             complement(55715..55975)
                     /gene="rof"
                     /locus_tag="ACH3L8_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFT4083415.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55962..56162)
                     /locus_tag="ACH3L8_000119"
     CDS             complement(55962..56162)
                     /locus_tag="ACH3L8_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFT4083416.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56328..>56471
                     /locus_tag="ACH3L8_000120"
     CDS             56328..>56471
                     /locus_tag="ACH3L8_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFT4083417.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWL"
BASE COUNT        13227 a        13947 c        15720 g        13577 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgttcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac caataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
    13021 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
    13201 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
    13321 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttctccgt tgttatggca gatgaatacc gcccacgcag cggctattga ccccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
    13801 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
    13921 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13981 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
    14041 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
    14281 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
    14401 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
    14461 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
    14521 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
    14761 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
    15661 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
    15781 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
    16381 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcctgcttc
    16441 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
    16501 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16561 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
    16621 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
    16681 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16741 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
    16801 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
    16861 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
    16921 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16981 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    17041 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
    17101 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
    17161 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17221 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
    17281 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17341 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17401 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
    17461 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17521 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17581 accgccaggg atcaactcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17641 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    17701 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17761 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17821 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17881 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17941 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    18001 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18061 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    18121 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    18181 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    18241 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    18301 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    18361 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18421 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18481 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18541 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18601 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18661 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18721 ttaccaaatg ggtactgatg gggggggcga ttggcggtct gtgtggattg ctggggtttg
    18781 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    18841 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    18901 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18961 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    19021 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    19081 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19141 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga
    19201 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    19261 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    19321 gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    19381 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    19441 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    19501 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    19561 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca
    19621 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    19681 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    19741 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    19801 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    19861 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    19921 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat
    19981 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    20041 cgcattaaag gctttattca cgctaatgcc gacaaacacc gccagaccaa cggcgtccag
    20101 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    20161 gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    20221 atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag
    20281 aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac
    20341 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg
    20401 aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga
    20461 taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga
    20521 gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa
    20581 tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc
    20641 tgtctttaaa gatgttttct ccgccacaaa cttcgagtac caggttctga atcgactctt
    20701 ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat
    20761 cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg
    20821 ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa
    20881 tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc
    20941 gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt
    21001 ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt
    21061 cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg
    21121 cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca
    21181 gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca
    21241 accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac
    21301 tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg
    21361 cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg
    21421 cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca
    21481 cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg
    21541 tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg
    21601 acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt
    21661 ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac
    21721 agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc
    21781 tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg
    21841 ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt
    21901 ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg
    21961 gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa
    22021 ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat
    22081 cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa
    22141 gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat
    22201 ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc tgaaagagct
    22261 taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga
    22321 gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc
    22381 gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac
    22441 tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga
    22501 gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg
    22561 tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa
    22621 atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa
    22681 aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa
    22741 tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc
    22801 ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct
    22861 ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt
    22921 ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca
    22981 gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg
    23041 atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga
    23101 tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt
    23161 tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt
    23221 agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa
    23281 agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca
    23341 tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct
    23401 atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct
    23461 ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag gcttttgtaa
    23521 agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa
    23581 acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa
    23641 aaaaaacaca ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct
    23701 ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc
    23761 accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac
    23821 cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt
    23881 ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa
    23941 tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga
    24001 taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca
    24061 gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat
    24121 cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga
    24181 tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac
    24241 ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga
    24301 aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa
    24361 cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat
    24421 cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga
    24481 atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact
    24541 ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa
    24601 ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa
    24661 gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa
    24721 actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca
    24781 gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat
    24841 gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc
    24901 tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa
    24961 aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat
    25021 tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg
    25081 aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca
    25141 tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc
    25201 ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg
    25261 cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta
    25321 tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt
    25381 cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc
    25441 aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag
    25501 gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc
    25561 tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg
    25621 tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct
    25681 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25741 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25801 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25861 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25921 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg
    25981 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    26041 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26101 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26161 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    26221 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    26281 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26341 tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag gcggcgctgg
    26401 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    26461 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26521 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26581 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26641 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26701 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26761 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26821 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26881 tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26941 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    27001 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    27061 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga
    27121 aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag
    27181 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    27241 gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc
    27301 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    27361 aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc
    27421 agagcgtgcg ccaatgaagc agttatcttc aataatggtc ggggtagcct gcagcggctc
    27481 cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    27541 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    27601 aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    27661 tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    27721 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    27781 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    27841 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    27901 gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    27961 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    28021 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    28081 attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    28141 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    28201 cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    28261 ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg
    28321 acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat
    28381 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    28441 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg
    28501 gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat
    28561 ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc ttaaatcatg
    28621 ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
    28681 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
    28741 tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg
    28801 cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
    28861 ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag
    28921 atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc
    28981 ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
    29041 aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg
    29101 agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
    29161 gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg
    29221 ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
    29281 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg
    29341 cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc gccgcaccgc
    29401 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg
    29461 ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
    29521 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
    29581 aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgag
    29641 tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
    29701 catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc
    29761 gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc
    29821 gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca
    29881 cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
    29941 gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
    30001 aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt agctggtgcc
    30061 atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga acttgtcgga
    30121 aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc
    30181 aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccaa
    30241 catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag
    30301 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
    30361 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcaac
    30421 caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg
    30481 gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
    30541 atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga cggttaattc
    30601 atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga gagcggtgtt
    30661 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
    30721 aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg
    30781 tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca atagtatgct
    30841 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
    30901 ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc cccggtttca
    30961 gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
    31021 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt
    31081 tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca
    31141 ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc ggcttaccga
    31201 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
    31261 taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt
    31321 cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg
    31381 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
    31441 ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
    31501 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
    31561 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
    31621 gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg
    31681 acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat ttgtggtata
    31741 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
    31801 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
    31861 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
    31921 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
    31981 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
    32041 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gtttctcgca
    32101 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    32161 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    32221 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    32281 gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    32341 tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    32401 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt attccggtat
    32461 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    32521 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    32581 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    32641 gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc
    32701 gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg
    32761 aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    32821 atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc
    32881 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    32941 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    33001 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    33061 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    33121 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    33181 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    33241 agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc
    33301 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt
    33361 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    33421 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    33481 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt
    33541 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    33601 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    33661 catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt
    33721 tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt
    33781 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    33841 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    33901 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    33961 actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa
    34021 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    34081 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    34141 accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    34201 ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc
    34261 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    34321 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    34381 gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagatccaac cgcaaccatg
    34441 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    34501 gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg
    34561 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    34621 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt
    34681 ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac
    34741 aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa
    34801 taatccagac gattacccgt aatatgttta atcagggcta tacttagcac acttccactg
    34861 tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat
    34921 atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc
    34981 agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat
    35041 tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact
    35101 ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcc
    35161 tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg
    35221 ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg
    35281 cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa
    35341 gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact
    35401 gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag
    35461 ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc
    35521 gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg
    35581 ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt
    35641 ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga
    35701 attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat
    35761 gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat
    35821 ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg ctgggctgtt
    35881 acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact
    35941 ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc
    36001 ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc agcataatct
    36061 gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg
    36121 ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcttgccg
    36181 tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa
    36241 aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt
    36301 gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct
    36361 ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa
    36421 tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc
    36481 cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg
    36541 ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc
    36601 gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga
    36661 tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgagtgaagt
    36721 cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg
    36781 ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg
    36841 ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat
    36901 tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa
    36961 ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc
    37021 aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca
    37081 aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg
    37141 tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa
    37201 ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg
    37261 cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat tcgtaaatct
    37321 gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga
    37381 cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg
    37441 caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca tgaactggcc
    37501 cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt tttgctttgg
    37561 caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa
    37621 gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag
    37681 cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct
    37741 tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc
    37801 attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt
    37861 accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg
    37921 ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt
    37981 tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc
    38041 gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt
    38101 cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt
    38161 ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat
    38221 atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc
    38281 tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg ttatggcatg
    38341 tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta
    38401 gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac
    38461 agcggtcatt taattccagg acacggtggt attttagatc gtattgatag cctgacggct
    38521 gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta
    38581 atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca
    38641 ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt
    38701 tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg
    38761 ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg
    38821 tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga
    38881 ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt
    38941 ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc aattcgatag
    39001 ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc
    39061 ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca
    39121 ttacagtagc gccatttgac agcgaccaac ggcgggatgt aaagctcgat ttacgtcact
    39181 gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc
    39241 cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt
    39301 tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct
    39361 ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg
    39421 ggagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt aaagcgattg
    39481 gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc
    39541 agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc
    39601 tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg
    39661 ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc
    39721 tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg
    39781 tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat
    39841 ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg
    39901 ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat
    39961 aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata
    40021 cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt
    40081 tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg atgaagatat
    40141 cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg
    40201 tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt
    40261 ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt
    40321 gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga
    40381 cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc
    40441 gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca
    40501 aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact
    40561 gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga aacagaaact
    40621 ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg cccgtttcaa
    40681 catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa
    40741 catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg
    40801 gcactccgct gaaattgagc aactgactaa gatcgagccg ggcgagctgt ataacggcac
    40861 taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg gttatgccta
    40921 tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt
    40981 gaacgttgat gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac
    41041 ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggttggggag
    41101 cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga
    41161 taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa
    41221 agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag
    41281 cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa
    41341 cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac
    41401 cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc
    41461 cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat
    41521 taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat
    41581 gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc
    41641 tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa
    41701 caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt
    41761 gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt
    41821 gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga
    41881 tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac
    41941 cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc
    42001 cagtaattat gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct
    42061 gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat
    42121 caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt cattcttctg
    42181 ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg gatatccgga
    42241 ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc
    42301 attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc
    42361 agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag
    42421 tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact
    42481 gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg
    42541 caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca
    42601 atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa
    42661 atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa
    42721 tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa aaagacgtga
    42781 tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc
    42841 gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg
    42901 ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg
    42961 atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc
    43021 gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca
    43081 tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc
    43141 caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg
    43201 atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc
    43261 gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg
    43321 tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg
    43381 agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa
    43441 acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc
    43501 agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt
    43561 atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg
    43621 atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta
    43681 ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg
    43741 acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact
    43801 gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg
    43861 ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca
    43921 ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg
    43981 acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca
    44041 tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta
    44101 ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct
    44161 gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg
    44221 cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga
    44281 gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga
    44341 agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg
    44401 tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg
    44461 cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa
    44521 aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag
    44581 ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga
    44641 agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca
    44701 tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa
    44761 aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta accaggattt
    44821 gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag
    44881 cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa
    44941 cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct
    45001 cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg
    45061 catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc
    45121 cggtgtggcg caggacgtcc ctccttatgt tattgcgcag ggtaaccacg caacgccgtt
    45181 cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat
    45241 ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga
    45301 aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg
    45361 ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat tgccctggtc
    45421 gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt
    45481 gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa
    45541 gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct cggtcgtctg
    45601 cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat
    45661 gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag
    45721 cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc
    45781 gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg
    45841 ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg
    45901 ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac
    45961 tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc
    46021 ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg
    46081 gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctg
    46141 tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga tgcggcacta
    46201 ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga
    46261 tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc
    46321 tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt
    46381 gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac
    46441 gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag
    46501 gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc
    46561 tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg
    46621 cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg
    46681 aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg
    46741 gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga
    46801 tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc
    46861 gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc cgcgtaccag
    46921 aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg
    46981 atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc gcttttcatc
    47041 tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca
    47101 aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga
    47161 agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg
    47221 gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg
    47281 cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg
    47341 cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg ctgggtgatg
    47401 agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat ctggcgttgc
    47461 tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact
    47521 ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg
    47581 gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag
    47641 aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg gatgaagaaa
    47701 gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta
    47761 acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc
    47821 acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata
    47881 tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc cttgccaaca
    47941 ccgttgagat cgccaaacgc tgtaacgtaa ctgtgcgtct tggtgaatac ttcctgccgc
    48001 agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca aaagaaggcc
    48061 tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa cgccgcccgg
    48121 aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct
    48181 acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc
    48241 caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg
    48301 atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc
    48361 ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa cacgtggcgg
    48421 acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag
    48481 cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gaccgtatct
    48541 cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc
    48601 agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca
    48661 aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga
    48721 ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc
    48781 agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc
    48841 gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg
    48901 gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc
    48961 tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga
    49021 tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc
    49081 ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgtgaag
    49141 agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta ctggagccaa
    49201 cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt
    49261 ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag ccggaagaga
    49321 tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac
    49381 tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc
    49441 actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg
    49501 cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc
    49561 tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc
    49621 tttaccattt ccacgtcaac gacgatggcg aaatcgtgta tggtattggc gccatcaaag
    49681 gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc
    49741 gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg
    49801 aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga
    49861 actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc
    49921 aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca
    49981 gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggcctgt
    50041 acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg
    50101 taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg
    50161 ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg
    50221 atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc
    50281 aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca
    50341 gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat
    50401 atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc
    50461 gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc
    50521 agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg
    50581 atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg
    50641 actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc
    50701 tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta
    50761 acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct
    50821 tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag cgtccttata
    50881 ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgtgcgt
    50941 atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga
    51001 tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc
    51061 cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga
    51121 tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc
    51181 gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt ggcgtaccgg
    51241 tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg
    51301 ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt
    51361 ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg
    51421 ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg
    51481 ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg gcggatctgg
    51541 ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga
    51601 gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt
    51661 tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacatgaa
    51721 catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca tcaaagaact
    51781 ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca gcgttgattt
    51841 gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact gggatgagta
    51901 cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc tttatgcctt
    51961 catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg cgctctggtt
    52021 ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc agtacaccaa
    52081 cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga
    52141 gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc aaaaaagccc
    52201 ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg atgtctctat
    52261 ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacacctgg aagcggaaga
    52321 gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg gaatatcgac
    52381 gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt tgatcgaccg
    52441 caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc cagtctggct
    52501 gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg aatttactcg
    52561 cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg ttcatgcggt
    52621 gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca aacagacgct
    52681 ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt ttcatccaat
    52741 ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac
    52801 gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc acattaaagg
    52861 cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca cctcgcccag
    52921 ttatcccatt gttgcttcgg ttaagacggc ggcggcgatg ctgcgtggta atccgggcaa
    52981 acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg tccagcggct
    53041 gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg tggatgaagc
    53101 cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg cggatgccga
    53161 tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg acgaacaggg
    53221 caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg acgaacgtgg
    53281 gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta ttggcatcga
    53341 taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct cttacgatct
    53401 caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg atttctaccg
    53461 caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta aacacgatct
    53521 ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga cgccacatca
    53581 ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgccggaac aactggtcgg
    53641 tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt tgatgccagg
    53701 agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc tttgttccgt
    53761 agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg acgaagacgg
    53821 cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg cttccgggcg
    53881 agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca ggttcaccat
    53941 attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga tattcttggt
    54001 ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg
    54061 cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga gcgccccagc
    54121 cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat cgatgcggca
    54181 gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga tccatacacg
    54241 caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact gtatgagcag
    54301 taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg
    54361 caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga ttctggtggc
    54421 ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt ggcggacgga
    54481 aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg ccaatgccga
    54541 tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga
    54601 acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc aggcacgtta
    54661 tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc aacatctcga
    54721 cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg ccgggctttc
    54781 ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt tgcttgcccg
    54841 cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga ttgaagacga
    54901 aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt
    54961 gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac tttgtgctga
    55021 acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc aaacgtcgca
    55081 ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg cggcgattat
    55141 tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc tggtgcgcat
    55201 ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt tgggcgcctt
    55261 tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg ggcaaagcga
    55321 aaccattgtg ctgtggcaga cgtggctaca accgctggaa ttaccggcgg ggctgggaac
    55381 cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt
    55441 gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa
    55501 gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt
    55561 ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag gtgtggctga
    55621 aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga aagccggata
    55681 agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc accaccgtac
    55741 cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg gtttcgccag
    55801 cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcactg gcttttgcct
    55861 gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg caggcgagct
    55921 caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt gacataatcg
    55981 ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc agaaagggcg
    56041 tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat
    56101 cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca atatttttcc
    56161 acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc ctacgaagat
    56221 aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag cgccgcaact
    56281 gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag
    56341 cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg
    56401 cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg ctgcgcttgc
    56461 tggcgtggct g
//
LOCUS       DAZXUK010000003        53000 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZXUK010000003 DAZXUK010000000
VERSION     DAZXUK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53000
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.003"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            239..325
                     /gene="leuL"
                     /locus_tag="ACH3L8_000121"
     CDS             239..325
                     /gene="leuL"
                     /locus_tag="ACH3L8_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFT4083418.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            418..1989
                     /gene="leuA"
                     /locus_tag="ACH3L8_000122"
     CDS             418..1989
                     /gene="leuA"
                     /locus_tag="ACH3L8_000122"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFT4083419.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            1989..3080
                     /gene="leuB"
                     /locus_tag="ACH3L8_000123"
     CDS             1989..3080
                     /gene="leuB"
                     /locus_tag="ACH3L8_000123"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFT4083420.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3083..4483
                     /gene="leuC"
                     /locus_tag="ACH3L8_000124"
     CDS             3083..4483
                     /gene="leuC"
                     /locus_tag="ACH3L8_000124"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFT4083421.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4494..5099
                     /gene="leuD"
                     /locus_tag="ACH3L8_000125"
     CDS             4494..5099
                     /gene="leuD"
                     /locus_tag="ACH3L8_000125"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFT4083422.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(5148..6326)
                     /gene="setA"
                     /locus_tag="ACH3L8_000126"
     CDS             complement(5148..6326)
                     /gene="setA"
                     /locus_tag="ACH3L8_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFT4083423.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(6428..6559)
                     /gene="sgrT"
                     /locus_tag="ACH3L8_000127"
     CDS             complement(6428..6559)
                     /gene="sgrT"
                     /locus_tag="ACH3L8_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFT4083424.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6648..8303
                     /gene="sgrR"
                     /locus_tag="ACH3L8_000128"
     CDS             6648..8303
                     /gene="sgrR"
                     /locus_tag="ACH3L8_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFT4083425.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8337..8436
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8467..9450
                     /gene="thiB"
                     /locus_tag="ACH3L8_000129"
     CDS             8467..9450
                     /gene="thiB"
                     /locus_tag="ACH3L8_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFT4083426.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9426..11036
                     /gene="thiP"
                     /locus_tag="ACH3L8_000130"
     CDS             9426..11036
                     /gene="thiP"
                     /locus_tag="ACH3L8_000130"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFT4083427.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11020..11718
                     /gene="thiQ"
                     /locus_tag="ACH3L8_000131"
     CDS             11020..11718
                     /gene="thiQ"
                     /locus_tag="ACH3L8_000131"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFT4083428.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11832..12596)
                     /locus_tag="ACH3L8_000132"
     CDS             complement(11832..12596)
                     /locus_tag="ACH3L8_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFT4083429.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12682..13560)
                     /gene="araC"
                     /locus_tag="ACH3L8_000133"
     CDS             complement(12682..13560)
                     /gene="araC"
                     /locus_tag="ACH3L8_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFT4083430.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13899..15599
                     /gene="araB"
                     /locus_tag="ACH3L8_000134"
     CDS             13899..15599
                     /gene="araB"
                     /locus_tag="ACH3L8_000134"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFT4083431.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15610..17112
                     /gene="araA"
                     /locus_tag="ACH3L8_000135"
     CDS             15610..17112
                     /gene="araA"
                     /locus_tag="ACH3L8_000135"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFT4083432.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17312..18007
                     /gene="araD"
                     /locus_tag="ACH3L8_000136"
     CDS             17312..18007
                     /gene="araD"
                     /locus_tag="ACH3L8_000136"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFT4083433.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18082..20433
                     /gene="polB"
                     /locus_tag="ACH3L8_000137"
     CDS             18082..20433
                     /gene="polB"
                     /locus_tag="ACH3L8_000137"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFT4083434.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20598..23504
                     /gene="rapA"
                     /locus_tag="ACH3L8_000138"
     CDS             20598..23504
                     /gene="rapA"
                     /locus_tag="ACH3L8_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFT4083435.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23516..24175
                     /gene="rluA"
                     /locus_tag="ACH3L8_000139"
     CDS             23516..24175
                     /gene="rluA"
                     /locus_tag="ACH3L8_000139"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFT4083436.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24292..25107)
                     /gene="djlA"
                     /locus_tag="ACH3L8_000140"
     CDS             complement(24292..25107)
                     /gene="djlA"
                     /locus_tag="ACH3L8_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFT4083437.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25362..27716
                     /gene="lptD"
                     /locus_tag="ACH3L8_000141"
     CDS             25362..27716
                     /gene="lptD"
                     /locus_tag="ACH3L8_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFT4083438.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27769..29055
                     /gene="surA"
                     /locus_tag="ACH3L8_000142"
     CDS             27769..29055
                     /gene="surA"
                     /locus_tag="ACH3L8_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFT4083439.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29055..30044
                     /gene="pdxA"
                     /locus_tag="ACH3L8_000143"
     CDS             29055..30044
                     /gene="pdxA"
                     /locus_tag="ACH3L8_000143"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFT4083440.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30041..30862
                     /gene="rsmA"
                     /locus_tag="ACH3L8_000144"
     CDS             30041..30862
                     /gene="rsmA"
                     /locus_tag="ACH3L8_000144"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFT4083441.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30865..31242
                     /gene="apaG"
                     /locus_tag="ACH3L8_000145"
     CDS             30865..31242
                     /gene="apaG"
                     /locus_tag="ACH3L8_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFT4083442.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31249..32091
                     /gene="apaH"
                     /locus_tag="ACH3L8_000146"
     CDS             31249..32091
                     /gene="apaH"
                     /locus_tag="ACH3L8_000146"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFT4083443.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32169..32648)
                     /gene="folA"
                     /locus_tag="ACH3L8_000147"
     CDS             complement(32169..32648)
                     /gene="folA"
                     /locus_tag="ACH3L8_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFT4083444.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32839..34701)
                     /gene="kefC"
                     /locus_tag="ACH3L8_000148"
     CDS             complement(32839..34701)
                     /gene="kefC"
                     /locus_tag="ACH3L8_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFT4083445.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREA
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34694..35224)
                     /gene="kefF"
                     /locus_tag="ACH3L8_000149"
     CDS             complement(34694..35224)
                     /gene="kefF"
                     /locus_tag="ACH3L8_000149"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFT4083446.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35272..36666)
                     /locus_tag="ACH3L8_000150"
     CDS             complement(35272..36666)
                     /locus_tag="ACH3L8_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4083447.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(36725..37012)
                     /gene="fixX"
                     /locus_tag="ACH3L8_000151"
     CDS             complement(36725..37012)
                     /gene="fixX"
                     /locus_tag="ACH3L8_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFT4083448.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37009..38295)
                     /locus_tag="ACH3L8_000152"
     CDS             complement(37009..38295)
                     /locus_tag="ACH3L8_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFT4083449.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38346..39287)
                     /locus_tag="ACH3L8_000153"
                     /pseudo
     CDS             complement(38346..39287)
                     /locus_tag="ACH3L8_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(39302..40072)
                     /locus_tag="ACH3L8_000154"
     CDS             complement(39302..40072)
                     /locus_tag="ACH3L8_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFT4083450.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            40428..41942
                     /gene="caiT"
                     /locus_tag="ACH3L8_000155"
     CDS             40428..41942
                     /gene="caiT"
                     /locus_tag="ACH3L8_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFT4083451.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            41973..43115
                     /gene="caiA"
                     /locus_tag="ACH3L8_000156"
     CDS             41973..43115
                     /gene="caiA"
                     /locus_tag="ACH3L8_000156"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFT4083452.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            43244..44461
                     /gene="caiB"
                     /locus_tag="ACH3L8_000157"
     CDS             43244..44461
                     /gene="caiB"
                     /locus_tag="ACH3L8_000157"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFT4083453.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            44535..46088
                     /gene="caiC"
                     /locus_tag="ACH3L8_000158"
     CDS             44535..46088
                     /gene="caiC"
                     /locus_tag="ACH3L8_000158"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFT4083454.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            46197..46982
                     /gene="caiD"
                     /locus_tag="ACH3L8_000159"
     CDS             46197..46982
                     /gene="caiD"
                     /locus_tag="ACH3L8_000159"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFT4083455.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            46988..47578
                     /gene="caiE"
                     /locus_tag="ACH3L8_000160"
     CDS             46988..47578
                     /gene="caiE"
                     /locus_tag="ACH3L8_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFT4083456.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(47664..48059)
                     /gene="caiF"
                     /locus_tag="ACH3L8_000161"
     CDS             complement(47664..48059)
                     /gene="caiF"
                     /locus_tag="ACH3L8_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFT4083457.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMCR"
     gene            complement(48321..51542)
                     /gene="carB"
                     /locus_tag="ACH3L8_000162"
     CDS             complement(48321..51542)
                     /gene="carB"
                     /locus_tag="ACH3L8_000162"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFT4083458.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(51560..52708)
                     /gene="carA"
                     /locus_tag="ACH3L8_000163"
     CDS             complement(51560..52708)
                     /gene="carA"
                     /locus_tag="ACH3L8_000163"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFT4083459.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
BASE COUNT        12705 a        14425 c        13921 g        11949 t
ORIGIN      
        1 gtcccctttt tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt caaccgatga
       61 aaagcgtcgg tagttaagca gaaattaata tcgcttactt taaccaccgt agcacaatta
      121 gctaatttta cggatgcaga actcacgctg gcgggacgtt tttattgcgt cagggttgac
      181 atccgttttt gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat
      241 gactcacatc gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag
      301 acgagtgagc ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt
      361 gcgcgggttt ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg
      421 agccagcaag tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca
      481 agcttgagtg tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac
      541 gtgatggaag tcggtttccc cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc
      601 gcccgccagg ttaaaaacag ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc
      661 gacgtggcgg ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgct
      721 acctccccaa tgcacatcgc caccaagctg cgcagcaccc tagacgaggt gatcgaacgc
      781 gctatctata tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa
      841 gatgccgggc gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc
      901 ggtgccacca ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc
      961 ggaatcatca gcggcctgta tgaacgcgta cctaacatcg acaaagccat tatttccgta
     1021 catacccacg acgatttagg cctggcggtc ggcaactcac tggcggcggt acatgccggt
     1081 gcacgccagg tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg
     1141 gaagaagtca tcatggcgat taaagttcgt aaggatattc tcaacgtcca caccgccatt
     1201 aatcaccaag agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc
     1261 ccggcaaaca aagccattgt tggcagcggc gcattcgctc actcctccgg tatccaccag
     1321 gatggcgtgc tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg
     1381 aaccaaatcc agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg
     1441 gatgagatgg ggtataaaga aagtgaatat aatttagaca atttgtacga tgctttcctg
     1501 aagctggcgg acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc
     1561 ggtaagcagc aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc
     1621 tctaacgata tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa
     1681 gccgccaacg gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgac
     1741 tataacgtcg aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg
     1801 ggtcaggtgg atattgtcac caactacaac ggtcgccgct tccacggcgt cggcctggcc
     1861 accgatattg tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgt
     1921 gccgcagaag tcgaaaaaga gttgcaacgc aaaactcaac acaacgaaaa caacaaggaa
     1981 accgtgtgat gtcgaagaat taccatattg ccgtattgcc gggagacggt attggtccgg
     2041 aagtgatgac ccaggcgctg aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca
     2101 tcaccaccag ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc
     2161 cgcctgcgac ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg
     2221 gcccgaagtg ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc
     2281 tgcgtaagca cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat caggggctgg
     2341 aagcattctg tccgctgcgt gcagacattg ccgctaacgg cttcgacatc cagtgcgtgc
     2401 gcgaactgac cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat
     2461 atgaaaaagc gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca
     2521 tcgcgtttga atctgctcgt aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg
     2581 tgctgcaatc ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg
     2641 atgtcgaact ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat
     2701 cacagtttga cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg
     2761 caatgatcac tggctcgatg ggcatgttgc cttccgccag cctgaacgag caaggttttg
     2821 gactgtatga accggcgggc ggctcggcac cagatatcgc aggcaaaaac atcgccaacc
     2881 cgattgcaca aatcctgtcg ctggcactgc tgctgcgtta cagcctggat gccgatgatg
     2941 cggcttccgc cattgaacgc gccattaacc gtgcattaga agaaggcatt cgcaccgggg
     3001 atttagcccg tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct
     3061 atgtggcaga aggggtgtaa tcatggctaa aacgttatac gaaaaattat tcgacgctca
     3121 cgtagtgtac gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca
     3181 tgaagtgacc tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca
     3241 gccgggcaaa accttcgcca ctatggatca caacgtctct acccagacca aagacattaa
     3301 tgcctgtggt gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt
     3361 tggcgtcgag ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc
     3421 ggaacagggc gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac
     3481 ccacggcgcg tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact
     3541 ggcaacacaa accctgaaac agggccgtgc gaagaccatg aaaattgaag tccagggcaa
     3601 agccgcgccg ggcattaccg caaaagatat cgtgctggca attatcggta aaactggcag
     3661 cgcaggcggc accgggcatg tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat
     3721 ggaaggtcgt atgaccctgt gcaatatggc gatcgaaatg ggcgcaaaag ccgctctggt
     3781 tgctcctgac gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa
     3841 agatttcgac gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt
     3901 cgataccgtt gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa
     3961 tcccggccag gtgatttctg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc
     4021 ggttgaacgt gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggtattcc
     4081 gctgaccgaa gtggctatcg acaaagtgtt tatcggttcc tgtaccaact ctcgcattga
     4141 agatttacgc gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc
     4201 actggtggtt cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggataa
     4261 aatatttatt gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat
     4321 gaacaacgac cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc gtaactttga
     4381 aggccgccag gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc
     4441 tgctgtgacc ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag
     4501 agaaatttat caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg
     4561 atgcaatcat cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc
     4621 tgtttaacga ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc
     4681 tgaacttccc gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg
     4741 gctcctcgcg tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg
     4801 cgccgagttt tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga
     4861 aattaagcga tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc
     4921 atttcgacgt ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta
     4981 ccatcgatgc cttccgccgc cactgcatga tgaatggtct ggacagcatc ggcctgaccc
     5041 tgcagcacga cgacgctatt gcgtcttatg aagcaaaaca acctgcgttt atgaattaga
     5101 ccccttgccc ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta
     5161 acctttgcgg ttaaaaataa tgcgacaaca gaaataatcg caattaccca gtagacagca
     5221 aagtgccccc aactttgtgc aattgctccc tgaataacac cagccagaat taccccggta
     5281 gagatagtgt tggtaaataa ggtggtagct gcccccgctc ttccaggcat taaatcctga
     5341 aaccatagca tcccaatacc cgcaacaatg ccgataaata cagcgttaaa aagttgcagc
     5401 gtcatcaacg ccatacggct atgaaagaaa atcaatccag tgtaaaacag cactcctgcc
     5461 accactgcaa tgaccatcat tcgccgctta ccataacgtt tgacatagta gccagccaga
     5521 atcattgctg gtatttccag tccagctgcc gtccccatca ggaaacccgc cagtttgtct
     5581 ggcaatccta aatcgctact gatccacaat ggcatatcaa taatgtacat agtgttgcag
     5641 gtccacatta acgtcgaggc gacaaataac atccgtacgt tactatcctg ccagtcacct
     5701 tgcattgata aagcattttc cgacggcagt tctacccgcg ccacagacag aagcataaat
     5761 gcaatcaata ccagactgag tgtgaatatc ccggcggcaa tcgaaaacat cactgtaaag
     5821 ccgtaattca acgccagcat aaaggccagc ggtggaccga ttacccatgc cagagaaagc
     5881 tgcgcacgca tcaccgagct aaacatcacc acttctcgcg ccgagttatc cgcgtattcc
     5941 cgcgccagag caaataactg tggcattgcc gtattggcca gagatgccag aagcacacca
     6001 caggtgataa gcgtcagata atgacgatta aatgcaaaca gtagcgcatt gccaatagcc
     6061 atcaaacagc aaaatataat cagttttcgc cgatcgccct gactgtcaga acgttttgcc
     6121 aaccagaggc ttacgccgat cccagcaata gcattcaccg tataaaagag tccgatccag
     6181 aaaggttgcg cgccaacctc acgactcaga aataagctca atgtaggagc ctgtagcgcc
     6241 ccggccaccc ccatcataaa agcgaccagc ataaatgccg cgtaaacacc gttcatacgg
     6301 cgagccatcg tcattatcca gatcatacgt tcccttttta gcgcggcgag aataaaaaaa
     6361 accagcaggt ataatctgct ggcgggtgat tttacaccaa tactcagtca cacatgatgc
     6421 aggcaagtca actttcagaa ttgcggtcat cccactgcat cagtccttca agcatggtta
     6481 atcgttgtgg gacgcttaac caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt
     6541 gctgataaaa ctgacgcatg gggcacctcc ttgcttcatc gttaggaatt attgacttaa
     6601 tatagggaaa ataaaattgc tgtcttttgc gcaggagttc cccttttatg ccatctgctc
     6661 gtctgcaaca acagttcatc cgcctgtggc aatgctgcga gggtaaatcg caggacacaa
     6721 cgctcaacga actggcagcg ttattgagct gctcgcgtcg tcatatgcgt accctgctca
     6781 acaccatgca ggatcgcggc tggctgacgt gggaagcgga agtcgggcgc ggtaaacgct
     6841 cgcgtctgac attcctctat accgggctgg cgcttcagca acagcgggcg gaagacctgc
     6901 tggagcagga tcgtatcgac caactagtgc agttggttgg cgacaaagcg actgtgcggc
     6961 aaatgctggt ttctcatctg ggccgcagct tccgccaggg gcggcacatc ctgcgcgtgc
     7021 tctactatcg tccgttgcgt aatctgctac ctggcagcgc attgcgccgt tccgaaaccc
     7081 atattgcccg gcaaatcttc agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg
     7141 aagcagacat cgcccaccac tggcagcaaa tatcaccgct tcactggcgt ttctttttgc
     7201 gtccaggagt ccattttcat catggtcgtg aactggaaat ggacgacgtg atcgcctctt
     7261 taaaacgaat caatacgctg ccgctctatt cgcatattac ggacattgtg tcgccgacgc
     7321 cctggacgct ggatatccat ctcacgcagc cggaccgctg gttgccgtta ctgctggggc
     7381 aagttccggc gatgatcctg ccgtgcgaat gggaaaccct cagtaacttt gccagccatc
     7441 ctattggcac cggtccgtat gcggtgattc gcaacagcac caatcaactg aaaattcagg
     7501 cattcgatga cttcttcggt taccgggcat taatcgacga agttaacgtc tgggttctgc
     7561 cggaaattgc cgacgagcca gccggtggtc tgatgctaaa aggtccacag ggcgagaaaa
     7621 aagagattga aagccgcctg gaggaaggtt gctactattt actgttcgac agccgcaccc
     7681 atcgcggggc gaatcagcaa gtcagggact gggtaagcta tgtgctttct ccaactaatc
     7741 tggtctattt cgctgaggaa cagtaccagc aactgtggtt cccggcttat ggactgctcc
     7801 cccgttggca ccatgcccgc accataaaga gcgaaaaacc ggctggcctg gaaagcctca
     7861 ccctgacctt ttatcaggat cacagtgagc atcgggtgat tgccgggatc atgcagcaga
     7921 ttctggcaag tcaccaggtc acgctggaaa tcaaagagat cagctacgat cagtggcatg
     7981 aaggagagat cgagagcgat atctggctta atagcgccaa ctttacgctg ccgctggatt
     8041 tttcgctgtt cgcgcacttg tgcgaggtgc cgctgctcca acactgtatt ccgatcgact
     8101 ggcaagccga cgccgcccgc tggcgcaatg gcgaaatgaa cctggcgaac tggtgccagc
     8161 aactggtcgc cagtaaagca atggtgccgc ttatccacca ctggctgatc attcagggac
     8221 aacgcagtat gcgcggcctg cgtatgaata ccctcggctg gttcgatttt aaatcagcgt
     8281 ggtttgcgcc accggatcca tgattgctgg tagataacaa aatcactaca ctaacgccgt
     8341 tctcaacggg gtgccacgca aacgcgtgcg ctgagaaaat acccgtcgaa cctgatccgg
     8401 ataacgccgg cgaagggatt tgaggctcct tctcaagtcc tttgccactc ttttttgagg
     8461 tgcaaagtgt taaaaaaatg tcttcccctg ctgttgctgt gcacagcgcc cgttttcgct
     8521 aaacccgttc tgactgttta tacctacgat tccttcgccg ccgactgggg gcctggtcca
     8581 aagatcaaaa aagcttttga agccaactgt aactgcgaac tgaaactggt ggcgctggaa
     8641 gatggcgttt cgcttctcaa ccgtctacgg atggaaggca aaaatagtaa agccgatgtg
     8701 gtgctggggc tggataacaa tctgttagac gccgccagta aaaccggact atttgccaaa
     8761 agcggtgtgg cagcggatgc cgttaacgtt cccggcggct ggaataatga cactttcgtc
     8821 ccgtttgatt acggctattt cgccttcgtc tatgacaaga acaaactgaa aaacccgccg
     8881 caaagcctga aagaattggt tgagagcgat cagaactggc gggtgattta tcaggatccg
     8941 cgtaccagca cgccggggct gggtctgctg ttgtggatgc aaaaagtcta tggcgatgac
     9001 gccccacaag cctggcaaaa actggcgaag aaaaccgtca cggtcaccaa aggctggagc
     9061 gaagcctacg gcctgttttt aaaaggtgaa agcgatctgg tactgagtta caccacctct
     9121 ccggcttatc acattctcga agagaaaaaa gataactacg ccgccgcgaa ctttagcgaa
     9181 ggtcactatc tgcaggtgga agtcgccgcc cgcaccgctg ccagcaagca gccggagctg
     9241 gcgcaaaaat tcctccagtt tatggtttct ccggcttttc agaatgcgat cccaaccggt
     9301 aactggatgt atccggtggc aaatgtcacg ctgcctgccg ggtttgaaca attgaccaaa
     9361 ccggccacca cgctggagtt cacaccagct gaagtggcgg cacaacgtca ggcatggatt
     9421 agcgaatggc aacgcgccgt cagccgttaa ttcccggctg gttaattcca ggtgtaagcg
     9481 ccgccacgct ggtggtagcg gttgcgctcg cggcgtttct cgccctgtgg tggaacgcgc
     9541 cgcagggtaa ctgggtggca gtctggcagg acagctacct gtggcatgtg gtgcgcttct
     9601 ccttctggca ggcgtttctc tcggcgctac tctctgtcgt acccgcgata ttcctcgccc
     9661 gcgccctcta tcgcaggcgc tttccgggtc ggctggcgct gttgcgtctg tgcgcaatga
     9721 ccttgatcct cccggtgcta gttgctgttt tcggcattct tagcgtctat ggtcgtcagg
     9781 gctggctggc atcgctctgc caatcgctcg gtctggagtg gaccttttcg ccctacggcc
     9841 tgcaaggcat tttgctggcg cacgtatttt ttaatctgcc gatggcgagc cgcttattac
     9901 tccaggcact ggaaaacatt cccggcgaac aacgtcagct tgccgcccag cttgggatgc
     9961 gcggctggca ttttttccgc ttcgtcgaat ggccgtggct acggcgacaa atcccgccgg
    10021 ttgccgcgct tatctttatg ctctgtttcg ccagcttcgc caccgtgcta tcgctgggcg
    10081 gcggtccgca ggcaaccact atcgagctgg ctatttatca ggcgctgagt tacgactacg
    10141 atcctgcccg cgcggcgatg ctggcgctgc tccagatggt gtgttgcctt gggctggtgc
    10201 tgttgagtca gcgactaagt aaggccattg cgcccggcac cacgctgctg caaggctggc
    10261 gcgatccgga cgatcgtctg catagccgca tttgcgacac ggtgttaata gtgctggcgc
    10321 tgctgctgtt gctgccaccg ttgctggcgg tgatcgtcga tggggtaaat cgccagttgc
    10381 cggaagtgct ggcacaaccg gtgctgtggc aggcgctgtg gacctcgttg cgtattgcgc
    10441 tggcggcagg tgtattgtgc gtagtgctga ccatgatgct gctatggagc agtcgcgaac
    10501 tgcgggcgcg gcagaaaatg ctggcgggtc aggcgctgga gatgagcggc atgttgatcc
    10561 tcgccatgcc ggggattgtg ctggctaccg gcttcttttt actgctcaac aacactatcg
    10621 gcctgccaca atctgctgac ggcattgtga ttttcaccaa tgcgttaatg gcgatccctt
    10681 atgcgctgaa agtactggaa aacccgatgc gcgatatcac cgcccgctac agtatgttgt
    10741 gtcagtcgct gggcattgaa ggctggtcgc gcttaaaagt ggtcgagctg cgcgccctga
    10801 aacgtccact ggcgcaggcg ctggcttttg cctgcgtgct gtcgattggt gattttggcg
    10861 tagtggcgtt gttcggtaac gatgatttcc gtaccctgcc gttttatctc taccagcaaa
    10921 ttggctccta tcgcagccag gacggcgcgg tcaccgcgtt aattctgcta ctactctgtt
    10981 ttctgctgtt taccgtgatt gaaaaactac cggggcgaaa tgttaaaact gactgatatc
    11041 acctggcttt atcaccattt gccgatgcgt tttagcttaa cggtagaacg cggcgagcag
    11101 gtggcaatcc tcgggccaag cggcgcgggt aaaagtaccc tgctgaattt gattgccggt
    11161 tttctgacgc cagccagcgg ttcgctgact atcgatggtg ttgatcacac cacgacgccg
    11221 ccgtcacgcc gtccggtgtc gatgctgttt caggagaaca acctgttcag ccatctgacg
    11281 gtcgcgcaga acatcgggct ggggctaaat ccggggttga aactgaacgc ggcacagcag
    11341 gagaaaatgc acgcgattgc ccgtcagatg gggattgata atttaatggc gcggttaccg
    11401 ggcgagcttt ccggcggtca gcgacagcgc gtggcgttag cgcgttgtct ggtgcgcgaa
    11461 cagccgattt tattgctcga tgaaccgttc tctgcgctcg atccggcgtt acgtcaggag
    11521 atgctgacgc tggtgagcac gagctgccag cagcaaaaaa tgacgctatt gatggtgtcg
    11581 cacagtgtgg aagatgcggc gcggatcgcc acacgctcgg tggtggtcgc cgacgggcgc
    11641 atcgcctggc agggtaagac tgaagaattg ttgagcggta aggcgagtgc ttcggcaata
    11701 ttggggatta cgggttagta tgcactgtca gatgcggcgt gaacacctta tccgacctac
    11761 ggacagtgcg ttttgtaggc ctgataagac gcggtcagcg tcgcatgagg caacccgcac
    11821 aagacaatcc cctaaacccc aaccacttta cgcaaaatat cgatatacac cggcatcaac
    11881 gggtggcgaa ttaacaccac cagcgccacc acgccgatgg cagaaatcaa cggcgtcagc
    11941 cacaacaaac ggccgcgcga caaataatga cttaaacgat cggttgcttt accgctgcgc
    12001 cataaccgcc agcacagcca gccaccaacc cacaaaaata ccgccgtcgc cagcagcaac
    12061 catttaaact cgccgctctg cattccggct ggaatatcga tcgccgcgcc cgccagaatc
    12121 ccaggcagga agtaaaacgg tggccacagc aggcagccga taatattcgg cgtaatgaat
    12181 tttgccaccg gcagatccag cattcccgcc accattggca ccagcggacg cgtcgggcca
    12241 acaaagcgac cgaccagaat ggtgaacatg ctgtgttgat gcaacgcatg ttcagtttta
    12301 tcgagtaatg ctttgttttt cttcagaaat gaccagcgat gcaacggctt tttaaaacgc
    12361 cagcccagcc agaaggagat ccagtcgccc agcagacaac cgacaatccc tgccagccaa
    12421 gcgtgccaga aacttaactc gccgctgcca atcagcgctc ccagccccgc catcagcacc
    12481 gtaccgggta gaatcaaacc gaccagcgcc agcgactcca gaaaggccac caacaccacc
    12541 gccatcaatg aatacacggt agattgggta ataaagtgtt ccagcaatgc ttgcataatg
    12601 tgcctgtcaa atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc
    12661 aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc
    12721 acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa
    12781 atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt
    12841 gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat
    12901 ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc
    12961 gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc
    13021 gcgtacacga ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag
    13081 taacaattgc tcaagcagat tcatcgccag cagctccgaa tagcgccctt ccccttgccc
    13141 ggcgttaatg atttgcccaa acaggtcgct gaaatacggc tggtgcgctt catccgggcg
    13201 aaagaaaccc gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg
    13261 cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac aaccgtagtg
    13321 atgaatctct cctggcggga acagcaaaat atcacccggt cggcagacaa attctcgtcc
    13381 ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc
    13441 cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc
    13501 caccagatgg gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat
    13561 acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg
    13621 ccgtcactgc gtcttttact ggctcttctc gctaacccaa ccggtaaccc cgcttattaa
    13681 aagcattctg taacaaagca ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta
    13741 taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat
    13801 agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac
    13861 tgtttctcca tacccgtttt tttggatgga gtgaaacgat ggcgattgca attggcctcg
    13921 attttggcag tgattctgtg cgagctttgg cggtggactg cgccagcggt gaagagatcg
    13981 ccaccagcgt agagtggtat ccccgttggc aaaaagggca attttgtgat gccccgaata
    14041 accagttccg tcatcatccg cgtgactaca ttgagtcaat ggaagcggca ctgaaaaccg
    14101 tgcttgcaga gcttagcgtc gaacagcgcg cagctgtggt cgggattggc gttgacagta
    14161 ccggctcgac gcccgcaccg attgatgccg acggtaacgt gctggcgctg cgcccggagt
    14221 ttgccgaaaa cccgaacgcg atgttcgtat tgtggaaaga ccacactgcg gttgaagaag
    14281 cggaagagat tacccgtttg tgccacgcgc cgggcaacgt tgactactcc cgctatattg
    14341 gcggtattta ttccagcgaa tggttctggg caaaaatcct gcatgtgact cgccaggaca
    14401 gcgccgtggc gcaatctgcc gcatcgtgga ttgagctgtg cgactgggtg ccagctctgc
    14461 tttccggtac cacccgcccg caggatattc gtcgcggacg ttgcagcgcc gggcataaat
    14521 ctctgtggca cgaaagctgg ggcggcttgc cgccagccag tttctttgat gagctggacc
    14581 cgatcctcaa tcgccatttg ccttccccgc tgttcactga cacctggact gccgatattc
    14641 cggtgggcac cttatgcccg gaatgggcgc agcgtctcgg cctgcctgaa agcgtggtga
    14701 tttccggcgg cgcgtttgac tgccatatgg gcgcagttgg cgcaggcgca cagcctaacg
    14761 cactggtaaa agttatcggt acttccacct gcgacattct gattgccgac aaacagagcg
    14821 ttggcgagcg ggcagttaaa ggtatttgcg gtcaggttga tggcagcgtg gtgcctggat
    14881 ttatcggtct ggaagcaggc caatcggcgt ttggtgatat ctacgcctgg tttggtcgcg
    14941 tactcggctg gccgctggaa cagcttgccg cccagcatcc ggaactgaaa gcgcaaatca
    15001 acgccagcca gaaacaactg cttccggcgc tgaccgaagc atgggccaaa aatccgtctc
    15061 tggatcacct gccggtggtg ctcgactggt ttaacggtcg tcgcacgcca aacgctaacc
    15121 aacgcctgaa aggggtgatt accgatctta acctcgctac cgacactccg ctgctgttcg
    15181 gcggtttgat tgctgccacc gcctttggcg cacgcgcaat catggagtgc tttaccgatc
    15241 aggggatcgc cgtcaataac gtgatggcgc tgggcggcat cgcgcggaaa aaccaggtca
    15301 ttatgcaggc ctgctgcgac gtgctgaatc gcccgctgca aattgttgcc tctgaccagt
    15361 gctgtgcgct cggtgcggcg atttttgctg ccgtcgccgc gaaagtgcac gcagacatcc
    15421 catcagccca gcaaaaaatg gccagtgcgg tagagaaaac cctgcaaccg cgcagcgaac
    15481 aggcacaacg ctttgaacag ctttatcgcc gctatcagca atgggcgatg agcgccgaac
    15541 aacactatct tccaacttcc gccccggcac aggctgccca ggccgttgcg actctataag
    15601 gacacgataa tggcgatttt tgataattat gaagtgtggt ttgtcattgg cagccagcat
    15661 ctgtatggcc cggaaaccct gcgtcaggtc acccaacatg ccgagcacgt tgttaatgcg
    15721 ctgaatacgg aagcgaaact gccctacaaa ctggtgttga aaccgctggg caccacgccg
    15781 gatgaaatca ccgctatttg ccgcgacgcg aattacgacg atcgttgcgc tggtctggtg
    15841 gtgtggctgc acaccttctc cccggccaaa atgtggatca acggcctgac catgctcaac
    15901 aaaccgttgc tgcaattcca cacccagttc aacgcggcgc tgccgtggga cagtatcgat
    15961 atggacttta tgaacctgaa ccagactgca catggcggtc gcgagttcgg cttcattggc
    16021 gcgcgtatgc gtcagcaaca tgccgtcgtt accggtcact ggcaggataa acaagcccat
    16081 gagcgtatcg gctcctggat gcgtcaggcg gtctctaaac aggatacccg tcatctgaaa
    16141 gtctgccgat ttggcgataa catgcgtgaa gtggcggtca ccgatggcga taaagttgcc
    16201 gcacagatca agttcggttt ctccgtcaat acctgggcgg ttggctatct ggtgcaggtg
    16261 gtgaactcca tcagcgacgg cgatgttaac gcgctggtcg atgagtacga aagctgctac
    16321 accatgacgc ctgccacaca aatccacggc gaaaaacgac agaacgtgct ggaagcggcg
    16381 cgtattgagc tggggatgaa gcgtttcctg gaacaaggtg gcttccacgc gttcaccacc
    16441 acctttgaag atttgcacgg tctgaaacag cttccaggtc tgcccgtaca gcgtctgatg
    16501 cagcagggtt acggctttgc gggcgaaggc gactggaaaa ccgccgccct gcttcgcatc
    16561 atgaaggtga tgtcaaccgg tctgcagggc ggcacctcct ttatggagga ctacacctat
    16621 cacttcgaga aaggtaatga cctggtgctc ggctcccata tgctggaagt ctgcccgtcg
    16681 attgccgtag aagagaaacc gatcctcgac gttcagcatc tcggtattgg tggtaaggac
    16741 gatcctgccc gactgatctt caatacccaa accggtccag cgattgtcgc cagcctgatt
    16801 gatctcggcg atcgttaccg tctgctggtt aactgtatcg acacggtgaa aacaccgcac
    16861 tccctgccga aactgccggt ggcgaatgca ctgtggaaag cgcaaccgga tctgccaact
    16921 gcttccgaag cgtggatcct cgctggtggc gcgcaccata ccgtcttcag ccatgcgctg
    16981 aacctcaacg atatgcgcca gttcgccgag atgcacgaca ttgaaatcac ggtgattgat
    17041 aacgacaccc gcctgccagc gtttaaagac gcactgcgct ggaacgaagt gtattacgga
    17101 tttcgtcgct aagtagccac atcaggtatg taacgcctga tgcgacgctg acgcgtctta
    17161 tctggcctac acgctgcgat tttgtaggcc ggataagcaa agcgcatccg gcattcaacg
    17221 cctgatgcga cgctggcgcg tcttatcagg cctacgcgct gcgattttgt agaccggata
    17281 agcgaagcgc atccggcacg aaggagtcaa catgttaaaa gatctcaaac gccaggtatt
    17341 agaagccaac ctggcgctgc caaaacacaa cctggtcacg ctcacatggg gcaacgtcag
    17401 cgccgttgat cgcgagcgcg gcgtctttgt gatcaaacct tccggcgtcg attacagcgt
    17461 catgaccgct gacgatatgg tcgtggttag catcgaaacc ggtgaagtgg ttgaaggtac
    17521 gaaaaagccc tcctccgaca cgccaactca ccggctgctc tatcaggcat tcccctccat
    17581 tggcggcatt gtgcatacgc actcgcgcca cgccaccatc tgggcgcagg cgggtcagtc
    17641 gattccagca accggcacca cccacgccga ctatttctac ggcaccattc cctgcacccg
    17701 caaaatgacc gacgcagaaa tcaacggcga atatgagtgg gaaaccggta acgtcatcgt
    17761 agaaaccttt gaaaaacagg gtatcgatgc agcgcaaatg cccggcgttc tggtccattc
    17821 ccacggcccg tttgcatggg gcaaaaatgc cgaagatgcg gtgcataacg ccatcgtgct
    17881 ggaagaggtc gcttatatgg ggatattctg ccgtcagtta gcgccgcagt taccggatat
    17941 gcagcaaacg ctgctggata aacactatct gcgtaagcat ggcgcgaagg catattacgg
    18001 gcagtaatga ctgtataaaa ccacagccaa tcaaacgaaa ccaggctata ctcaagcctg
    18061 gttttttgat ggattttcag cgtggcgcag gcaggtttta tcttaacccg acactggcgg
    18121 gacaccccgc aagggacaga agtctccttc tggctggcga cggacaacgg gccgttgcag
    18181 gttacgcttg caccgcaaga gtccgtggcg tttattcacg ccgatcaggt tccccgcgct
    18241 cagcatattt tgcagggtga acaaggcttt cgtctgacac cgctggcgtt aaaggatttt
    18301 caccgccagc cggtgtatgg cctttactgt cgcgcccatc gccaattgat gaattacgaa
    18361 aagcgtctgc gtgaaggtgg cgttaccgtc tacgaggccg atgtgcgccc gccagaacgc
    18421 tatctgatgg agcggtttat cacctcaccg gtgtgggtcg agggtgatat gcacaatggc
    18481 actatcgtta atgcccgtct gaaaccgcat cccgactatc gtccgccgct caagtgggtt
    18541 tctatagata ttgaaaccac ccgccacggt gagctgtact gcatcggcct ggaaggctgc
    18601 gggcagcgca tcgtttatat gctggggccg gagaatggcg acgcctcctc gcttgatttc
    18661 gaactggaat acgtcgccag ccgcccgctg ctgctggaaa aactcaacgc ctggtttgca
    18721 aaccacgatc ccgatgtgat catcggctgg aacgtggtgc agttcgatct gcgaatgctg
    18781 caaaaacatg ccgagcgtta ccgtcttccg ctgcgtcttg ggcgcgataa tagcgagctg
    18841 gagtggcgcg agcacggctt taaaaacggc gtcttttttg cccaggctaa aggtcggcta
    18901 attatcgacg gtatcgaggc gctgaaatcc gcgttctgga atttctcttc attctcgctg
    18961 gaaactgtcg ctcaggagct attaggcgaa ggaaaatcta tcgataaccc gtgggatcga
    19021 atggacgaaa ttgaccgccg tttcgccgaa gataaacctg cgctggcaac ttataacctg
    19081 aaagattgcg agctggtgac gcagatcttc cacaaaactg aaatcatgcc atttttactc
    19141 gaacgagcaa cggtgaacgg cctgccggtg gatcggcacg gcggttcggt ggcagcgttt
    19201 ggtcatctct attttccgcg aatgcatcgc gctggttatg tcgcgcctaa tctcggcgaa
    19261 gtgccgccgc acgccagccc tggcggctac gtgatggatt cacggccagg gctttatgat
    19321 tcagtgctgg tgctggacta taaaagcctg tacccgtcga tcatccgcac ctttctgatt
    19381 gatcccgtcg ggctggttga aggcatggcg caacctgatc cagagcacag taccgaaggt
    19441 tttctcgatg cctggttctc acgggagaaa cattgcctgc cggagattgt gactaacatc
    19501 tggcacgggc gcgataaagc caaacgtcag ggtaataagc cgctttcgca ggcgctgaag
    19561 atcattatga atgcctttta tggcgtgctc ggcaccaccg cctgccgctt cttcgatccg
    19621 cgcctggcat cgtcgatcac catgcgtggt catcagatca tgcggcagac caaagcattg
    19681 attgaagcgc agggctacga cattatctac ggagataccg actcgacttt tgtctggcta
    19741 aaaggtgcac attcggaaga agaggcggcg aaaatcggtc gtgcactggt gcagcacgtt
    19801 aacgcctggt gggcggaaac gctgcaaaaa caacggctga ccagcgcatt agaactggag
    19861 tatgaaactc atttctgccg ttttcttatg ccaaccattc gcggagctga taccggcagt
    19921 aaaaagcgct acgccgggct gattcaggag ggcgacaagc agcggatggt gtttaaaggg
    19981 ctggaaaccg tgcgcaccga ctggacgccg ctggcccagc agtttcagca ggagctgtac
    20041 ctgcgtatct tccgcaacga gccatatcag gagtacgtcc gccaaaccat cgacaaactg
    20101 atggcgggtg aactggatgc gcggctggtt taccgtaaac gccttcgccg tccgctgagc
    20161 gaatatcagc gtaatgttcc gcctcatgta cgcgccgctc gccttgccga tgaagaaaat
    20221 caaaagcgcg gtcgcccctt gcaatatcag aatcgcggca ccattaagta cgtatggacc
    20281 accaacggcc cggagccgct ggactaccaa cgttcaccac tggattacga acactatctg
    20341 acccgccagc tacaacccgt ggcggaggga atactccctt ttattgagga taattttgct
    20401 acacttatga ccgggcaact tgggctattt tgagcaaaaa aaagagttcg ccagatacca
    20461 ttttgatgcg tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac
    20521 ctgaataaca ccactacctc ataagcacgg tagcgggtgg ttattgcctg caattaaaga
    20581 tatagagccg aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa
    20641 gcgaattggg acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc
    20701 catctactgg tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt
    20761 tcaaccctgg tgataccatt accagccatg acggctggca gatgcaagtc gaagaagtaa
    20821 aagaagaaaa tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg
    20881 tagccctgcg tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc
    20941 tgtttgccgg gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt
    21001 attccagcga acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga
    21061 tcccgcatca gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc
    21121 tggctgacga agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac
    21181 tgctctctgg cgctgctgaa cgtgtgctga ttatcgtccc ggaaacctta cagcatcagt
    21241 ggctggtaga aatgctgcgc cgtttcaacc tgcgctttgc tctgtttgat gatgagcgtt
    21301 atgccgaagc tcagcacgat gcttacaacc cgtttgacac cgaacagctg gtgatttgct
    21361 cgctggattt tgcccgtcgt agcaaacagc gcttggaaca tctctgtgaa gccgaatggg
    21421 acctgctggt ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg
    21481 aatatcaggc cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga
    21541 ccccggaaca gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc
    21601 gtttccacga ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg
    21661 ccgttgccat gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg
    21721 agatgatcgg cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag
    21781 atgcccagag cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc
    21841 gcgtgctgtt ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca
    21901 ccattaagct gccgctaccg acgcagtatc agacggctat taaagtctcc ggcattatgg
    21961 gcgcacgtaa aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc
    22021 aggaatttga aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga
    22081 tgggctacct gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca
    22141 ctgcgctgca actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc
    22201 acgaaggtat gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca
    22261 ccggcgcaca ggtactgctg tgctcagaaa tcggttctga aggacgtaac tttcagttcg
    22321 ccagtcacat ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg
    22381 gtcgtctgga tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga
    22441 aaaccgctca gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca
    22501 cctgcccgac cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg
    22561 cttcaccgga tcaaaccgaa ggctttgacg atctgatcaa aagctgccgc gagcaacatg
    22621 aagcgctgaa agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg
    22681 gtggcgaaaa agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc
    22741 tgatcgcctt cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg
    22801 acaacatgat cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt
    22861 cggaagatgg catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac
    22921 agtttattac ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg
    22981 ataccggtag cagcacgatt tcactgttaa aaaacaaagc gttgccggta ggtacgctgt
    23041 tggtggaact gatttatgtg gttgaagccc aggctccgaa gcagttgcag ctcaaccgct
    23101 tcctgccacc gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc
    23161 aggtagagtt tgaaaccttt aaccgccagc ttaacgcggt taaccgtcac accggcagca
    23221 aactggttaa cgccgtgcag caggatgttc acgctatcct tcaactgggt gaagcgcaga
    23281 tcgagaaatc tgcccgtgca ttgattgatg cagcgcgtaa cgaagccgac gaaaaactgt
    23341 ctgccgagct gtctcgtctg gaagctctgc gtgcagtgaa cccgaacatt cgtgacgacg
    23401 aactgaccgc cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggtt
    23461 ggcgtctgga tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg
    23521 gatggaaaac tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca
    23581 tattatggtg gtcaacaagc cgagcggttt gttgtcagtg ccgggtcgtc tggaagagca
    23641 caaagacagc gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg
    23701 tctggatatg gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga
    23761 gttaaaacgc cagttccgcg agcgcgagcc gaaaaagcag tatgtggccc gcgtctgggg
    23821 gcatccatcc cccgcagaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg
    23881 cccgaaacag aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt
    23941 ggagtatgcg gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca
    24001 tcaattgcgt gtgcatatgc tggcgctggg gcatccgatt ctcggcgatc gtttttatgc
    24061 atcaccagaa gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat
    24121 tacccatccg gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc
    24181 acgctggcgc agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc
    24241 gattgcctga tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa
    24301 ccctttctgc tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc
    24361 catctccatc atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact
    24421 catcagctta cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag
    24481 cacattacag gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc
    24541 agattgctgc tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg
    24601 gtcaaactgg gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc
    24661 attcgggtgc agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg
    24721 aattaagtca aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg
    24781 gtaattatct gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg
    24841 aaggttcatt cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc
    24901 tttggatttg gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg
    24961 ctcacgctgg ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc
    25021 aattaacagg cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac
    25081 gccaatgatt tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc
    25141 tatctcccac aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat
    25201 cgcctataat ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct
    25261 attcggacag gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt
    25321 cacgcgcaac gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca
    25381 ctctcctggc caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc
    25441 tcgcctcaca gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata
    25501 ccaatgactt acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg
    25561 ccgtgtttac tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag
    25621 tgcagctcca tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc
    25681 tcggtaatgt ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga
    25741 atctgaacac caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg
    25801 gtcgcggtaa agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata
    25861 acggtagctt tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa
    25921 ttattcatga ccgcgaagaa caagttgctg agatctggaa cgcccgcttt aaggtgggtc
    25981 cggtaccgat cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg
    26041 gtttcttgat cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat
    26101 attactggaa catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc
    26161 gtggcaacat catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga
    26221 tggaactgga ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca
    26281 gttcacgtcg ttggttattc tactggaacc actccggggt catggatcag gtgtggcgtt
    26341 tcaacgtcga ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt
    26401 acggttccag tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa
    26461 acttcaatgc caccgtttca accaagcagt tccaggtttt cagcgaacag aacaccagta
    26521 gctactcggc agagccgcag ttagacgtta attactacca gaatgatgtt ggtccgtttg
    26581 atacgcgtat ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag
    26641 caacccgtgt tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca
    26701 tcaataccga agcgaagttt ctggcaaccc attatcagca aaccaatctt gactggtata
    26761 actccagaaa cacgaccaag ctggacgaat ccgttaaccg cgtaatgccg caattcaaag
    26821 ttgacggcaa aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa
    26881 cgctggaacc gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata
    26941 actacgactc gtctctgctg caatctgact actctggcct gttccgggac cggacttacg
    27001 gcggtcttga ccgtattgcc tccgctaacc aagtgacgac cggtgtcaca tctcgcatat
    27061 atgatgatgc tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg
    27121 agtctcgcac tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg
    27181 tgtgggcagg cgacacctac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc
    27241 agtacgatac acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg
    27301 atgaagaccg tctggtacag ctgaattacc attacgccag cccggaatat attcaggcta
    27361 cgctgcctaa gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg
    27421 ctgtcgccag caggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca
    27481 ccaatgcgaa caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg
    27541 caattcgcgt cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg
    27601 tatatgacaa cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc
    27661 tgggtacgca agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg
    27721 ttgatttacc acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg
    27781 aaaacgctgc ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta
    27841 gtcgataaag tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga
    27901 ttaatgcagt cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg
    27961 acgctgcgcc accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg
    28021 cagaaaatgg gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg
    28081 aaacagaaca acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac
    28141 tacaacactt atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac
    28201 gaggtgcgtc gtcgcatcac catcctgcca caggaagtcg aatccctggc gcagcaggtg
    28261 ggtaaccaaa acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg
    28321 gaaaacccga cctctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat
    28381 caggcgcgta acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag
    28441 gcgctgaacg gcggccagat ggggtggggc cgtattcagg agttgccggg gatcttcgcc
    28501 caggcattaa gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc
    28561 ttccatattc tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa
    28621 gttcatgctc gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgc
    28681 gtgaaactgg agcaaattgc tgctgatatc aagagtggta aaacgacttt tgctgccgca
    28741 gcgaaagagt tctcgcagga tccaggttct gctaaccagg gcggtgatct cggctgggct
    28801 acaccagata ttttcgatcc agccttccgt gacgccctga cccgcctgaa caaaggtcaa
    28861 atgagtgcac cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt
    28921 aatgtcgata aaaccgacgc tgcgcagaaa gatcgtgcat accgcatgct gatgaaccgt
    28981 aagttctcgg aagaagcagc aagctggatg caggaacaac gtgccagcgc ctacgttaaa
    29041 atcctgagca actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg
    29101 ggattggccc ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg
    29161 tttgtgccga tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc
    29221 tccgccctta ttcccccaac tcccctgcac aaccgcaaac tacgggcaca ttaacgctac
    29281 ttcctgtcgc gctacgtgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt
    29341 acgtggtgga gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc
    29401 tgatcacagg ccccgtgcat aaaggcgtca ttaacgacgc aagcattccg tttaccggtc
    29461 ataccgagtt tttcgaagag cgttcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg
    29521 aagaacttcg cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta
    29581 tcacccctgc acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat
    29641 ttggtattgc cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg
    29701 gtcatatggg tacggaagag atagacacca ttattccggt gctcgacgag ctgcgggcgc
    29761 aggggatgaa actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg
    29821 ataacgccga cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc
    29881 agggcttcgg gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg
    29941 accacggcac cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta
    30001 cggcgcttaa tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca
    30061 gggccactta gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga
    30121 cagcattgtt tctgccatta acccgcaaaa gggccaggca atggtcgaaa tcggccccgg
    30181 tctggcggca ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact
    30241 tgaccgcgat ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat
    30301 ttatcagcag gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc
    30361 gctgcgtgtt ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt
    30421 tagctatact gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg
    30481 tctggttgca ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta
    30541 ttgcaacgtg atcccggtac tggaagttcc gccgtcagcc tttacaccac cacccaaagt
    30601 ggattccgcc gtcgtgcgcc tggtccctca tgcaacgatg cctcacccgg ttaaagatat
    30661 tcgcgtgttg agccgcatca ccaccgaagc ctttaaccag cgtcgtaaaa ccattcgtaa
    30721 cagcctcggc aacctgttta gcgtcgaggt gttaaccgga atggggatcg acccggcgat
    30781 gcgagcggaa aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa
    30841 cgcgcctttg caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa
    30901 agcgtctaca ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc
    30961 gtaaccatac gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc
    31021 accaatggca atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca
    31081 cttatcgcgc ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg
    31141 ggcaccatgc agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac
    31201 attcccgtat tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac
    31261 cttattggcg acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa
    31321 tttacccccg ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cggcccgggt
    31381 tcgctggatg ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc
    31441 aatcacgatc tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat
    31501 cgcctgacac cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc
    31561 cagcctctgc tgcaaatcga cgaagagaaa aagctggtga tggcccacgc agggatcacg
    31621 ccgcagtggg atctgcagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctgtcg
    31681 agtgactcct atcccttctt tcttgacgct atgtacggcg atatgccaaa taactggtca
    31741 ccggaattgc gggggctggg aagactgcgt tttatcacca acgcctttac ccgtatgcgt
    31801 ttttgcttcc cgaacggtca actggatatg tacagcaaag aatcgccgga agaagcccct
    31861 gccccactga aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc
    31921 tttggtcact gggcatcgct ggaaggcaaa ggtacgccgg aaggtattta cgcgctggat
    31981 accggctgct gctggggtgg gtcattaacc tgcctgcgct gggaagataa acagtatttt
    32041 gtccagccgt cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct
    32101 gatacaggaa ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct
    32161 atacaaactt accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatcg
    32221 gcgtcgtgga attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg
    32281 gtgtcgcctt ccacttctgc gtcgatatgc gtcagataca gtttttgcgc ttttggcagg
    32341 aactgctcat aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc
    32401 gcaatggctt catccaccga tttcacccac gttacgcgat cgtccgtacc cggttgactg
    32461 ctgaggataa tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg
    32521 cccataatca cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg
    32581 ttccacggca tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga
    32641 ctgatcattg agatttcccg ataaaaaaat tgtcgccact atacgtaaag cgtaaaccgt
    32701 cgtcgactgg tgcgaggatg atgttgagga aaattttata ttctgctggc gagtccacgc
    32761 tctccccctg gactcgccgc attacaatga aacaaaaaca aacagttagc tgtaaagtgt
    32821 gatttacgtc actctttatt aagatgaggg tttcgtttcc ggttcatccg ccatgttgcc
    32881 ggtatgttta ccttcttctg ttccctgcca gccatgtcgt tgaattaatg acagatgttc
    32941 gcggtcctcg gtgatgatct cacttaacat cgcgctggtg cgtttataga ccgccgcgcg
    33001 ggctttggtg tcattctcaa ccattgccat ctcttccacc atctgaatat taaagcggcg
    33061 gaacacatcg gcacgttctc gcgcttcata cggccccaga cctaaacttt ccagcgccag
    33121 acgcccggtt ttcagcgcac cttcaaaggt ttcacgctcc ggcttttcaa cgcctgcctg
    33181 acgcaaacga atgtagtgat cgacatcgcg ggcgcgggca ataatctgca aatgcgggaa
    33241 atgttctttc accatctccg tcagttgcag gttggtttgc ggatcgtcaa tagcgttaat
    33301 cagcacttcc gctttcgccg ctccggcaga ttccagtaaa tccatccgcg tggcatcgcc
    33361 ataaaacact ttcataccaa atttacgcag cgtttcgata tggtccggat cgtgatcgag
    33421 caccaccatt ttcaccccgc tggagagcag taaacgtccg gtaatctgtc caaaacgacc
    33481 gaatccggcg ataatcacgc gcggctgttc ttcatcgatc tcatcggctt cacgcgcctc
    33541 ctccgtagaa gattgctcaa ggcgattgag gatcaccagc agaatcggcg ttgctgccat
    33601 cgacagcgcc accgccaggg tcagcgattt cgcccactcc ggctccagca cattcgccat
    33661 ctgcgccgca ccaaatacca caaaggcaaa ctcactgccc tgccctaaca acaccgcaaa
    33721 ccagcgacgc tgtttatttg gcacctgcaa cggtcgggca atcagccaca gcatggcgat
    33781 tttgatgatg aggaaaccga gcagcaaaat gacaatgcgc aatgggtttt caatcagcgt
    33841 gccaaagtct atcgacatgc caacaccgat gaaaaacagc cccaacagca aacctttaaa
    33901 cggttcgata tcgctttcca acgcatgacg gtattccgag cttgccagca gtacgcccgc
    33961 cagaaacgcg cccatcgcca tcgacaagcc gacctcttcc agcagcaaac caaagccaaa
    34021 cacaaggaat aacgccacgg cactaaacgc ttcccgcaag ccagagcggg caacaaaacg
    34081 cagcgccgga cgcgtaacat agcgccccag caataccacc agtaccagcg cacccgccac
    34141 ttttaacgcc gagagagcaa atgcgcccat cgtcgtcgag gcactgctcg ccgccagtag
    34201 cgggatcatc gccaccagcg ggattgccgc gatatcctgg aacagcagca ctgcaaaggc
    34261 actgcggccc atttgcgtca ccatcagatt gcgttcattc atcgcctgca tggcaatcgc
    34321 cgttgatgag agcgccagcg tcataccgat caattccgcg acctgccagc gcagcccaag
    34381 taacatacag aacagcccca gcaggccacc gcaaatcacc atctgcaatg cgccaccgcc
    34441 gaacactgcc gcacgcagct tccacagcct ttgtggatcg agttcgaggc cgataataaa
    34501 cagcatcagc accaccccaa tctcggcaaa gtgcagaata gattcggcat cggtcaccag
    34561 tcgcagcccc cacgggccaa taatgcagcc ggcgatcagg tagccaagta ccgatcccag
    34621 accaagacgt accgcaatgg gtacaatcag cgccgccgat ccgaggtaga tcagcgcctg
    34681 aaccagcgta tgactatcca tgatgggcct cctgccattc cagcagacgt tgcttatagt
    34741 ggcgcgcctg cccttcgagg gtttcgtcgt cacaaataaa ggtgcagtgc atggcaaacg
    34801 gtggcagcca gttcagcccg cagtagattg ccgtcgcctg tagcggctgc gacagcacat
    34861 caaagcccgg atgcgcacca atttcaaaat ggctttcccc gccgccggtc gtcaccgccc
    34921 acagcaaatg tttgccatgc agcgccgtgc cgccgtgacc gtaagcccag ccgtgcgaca
    34981 aaactttatc gatccaaagt ttaagaagcg gaggaatgct gtaccactgc atcgggtgct
    35041 gccagacgat cagatcggcg cgagagagcg cctcctgctc ggcggcaata tcgatattga
    35101 agtcaggata gagttgataa agagagcgaa tttcgacgcc ttccagcgtc cttgcctgtt
    35161 caagcatccg tttattcgca tgggaatgat gcggatacgg atgcgcataa atgatgagga
    35221 tcatagttat tagcccgcgt gtttacgcct ttgttttccc acaaagtgta gtcatatatt
    35281 cacgcggctt atagtcaata atattgattc agaaaagaga gaaatctgaa caaataatcc
    35341 cattctctgc ccgcggatcg tcatattgct ggtctgcgcc agtgacattc ctcgagtctc
    35401 cggggcaaac gctacggaaa tcaacaagcc aaacagcgag atacccgccc ccattagcat
    35461 cgtgttactg ataccgtaat tattgataaa tatcggcagt gcccaggtcg aaacaatggt
    35521 gccaatacga cttaaggaca taatcacgcc cacggcagag gcgcggatat ccgtcgggaa
    35581 gagttcatta ggatagagcc actgcaaatt acccggcccg ccagagaaaa aggcatacac
    35641 cgcaaaggcc atcactacca gccagatccc catatccggg attagcccca aaaccgccaa
    35701 cgccagcgtc atcatggcaa agctgccaat caacaatgga cgccgtccgg cagtgtttag
    35761 ccacagcatc ggcggaatac agccgagcat aaagaacagg ctaatcacca cattccctag
    35821 tgccgcgttt ttgccaaccc ccaatcccaa cgaaccaacg atttgcgggc caaaggtgta
    35881 aatggcgaac attgggatca cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg
    35941 aaaatggcgg cgattaaaca gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa
    36001 agccaccggt tcgccaaaca gttttatcat catctcttcg cactctttta ctcgcccttt
    36061 gcgtaataac cagcggggag attctggcag ttcgaatcga ccaatcaaaa tcaacaaaca
    36121 agggatcgcc gcgctaccca gcatccagcg ccaaccacct tccacatcat aaagccagta
    36181 gccgaccaga tcggcgcagg tcgcgccgac ataccacatc gcggcaataa agctgatgga
    36241 aaacgcccgc tgacgggtac tggagaactc ggtgatcatc gaagtggcga tgggataatc
    36301 tgcaccgatg acaatgccga tgagtacccg catcaccaac agttcgacgg gggatgaaac
    36361 acacatcgtc gccaccgata tcacgccgat ggcgatgata tcaatgagga acattttgcg
    36421 ccgtccgact ttatcggaga tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag
    36481 cgttcccgcg cccagcaagc caatccagtc agcgtccagt ttcagcgccg gagtcagttg
    36541 ctccagcgcc acgccaatca ttaccagtac ataaccatcc agaaacggac caccgcttcc
    36601 ccacagcaaa atgcggcggt gaatagagga gaatttgaga tcgtcaaagt ttctggacgg
    36661 ttgcatgggt tattcctgtt tttttgcaat gagagtcagt acggcgttac gtatcaaacc
    36721 aacatcagcc gtaacggaac tccacaccaa aggtgccgcg cgggtattcc cactgttcca
    36781 gcgccgaccc cagccccaga atgcgacagg tgccgcactc cagacatccg gcgtaatcga
    36841 agcgcacact gccgtcatcc tgcttcttgt acagacctgc ggggcacgct ttcaccagca
    36901 gctccagcac ctgtttatca gcatcggcct tcacaacaat gtgcggatgc tcttcatcga
    36961 cattgaattt attgacgccc agtttgacgt ccacattgac gggagaagtc ataaaacggt
    37021 cactcctttc atgccatcct tgatcagatt gatgaagccc actttcttgc cgtggcggag
    37081 gattttcttg cgcatcagtt ccggcgcact gccgtcaatg gtgaacaggt cacgcgccac
    37141 gcccaccgcc agttccgggt tgccgctaaa catgcgtggg ttatcaagga acgccggtag
    37201 tttctggtac atgcgcatat cgcgcagcgg gccactctcc agatgctgac gatattccgc
    37261 gagtttttgc ttactgaaat cgtcgctttt catcgctgaa agcacggttt ttgctgcagc
    37321 ttccccggcg gcaatcgcca gatccatacc gcgaatggta aaaccgaggt tcatacacat
    37381 tccggcggca tcaccggcaa tcaatacgcc gtcaccgacc agctccggca gcatgttgat
    37441 gcctgcttcc ggcactacgt gagcggaata ttccaccagc ttgccgcccg cgatcagcgg
    37501 tgcaacggcc ggatgctgtt tgaaatcttc cagcatttgc ggcaccgatt ttttcgcgtc
    37561 atgcagatga tgcagaccac aaaccagccc cagcgacagg gtattttcat tggtataaag
    37621 gaagccgccg cccatcaggc catcggtggg tgatcccgca aacagacaag ccgcgccctg
    37681 attaccctgc aactgaaaac ggtcttcgat taccgacttc ggtaactcga tcagttcctt
    37741 cacgccaacc gccacatccg tcggtttaac gcgttttgcc atccccaact tttcggcaag
    37801 gatggagttc accccatcag caaggagcac cgttttcgct tcaatcacat caccatcggc
    37861 ttctacaccg acgactttgc catcgcgctg tacgaggtta tcgacgcgga tcccggtaat
    37921 taactgcgcg cccgcttctt cggcctgctc catcaaccag gcatcaaatt tactgcgcaa
    37981 aacggagtaa gaacgctggg atggcgaggt ttcgtcaccg ttgcagtagt ccatagtcat
    38041 cgctgacttt tccgtcataa acgcgagttt ttcatgggtg atcaggcgtt ctacgggggc
    38101 ggaatcggcg aaacctggaa taatgtgttc cagactgtgg gcatagagac gcccgccagt
    38161 aacgttcttg gcacctgcgg aattgccacg ctcgataact aacacttgcg ccccttcgcg
    38221 ggcgagcacc agtgcggcaa ccgaaccggc aagccctgca ccgacgatga tggcgtcaaa
    38281 gatatcttcg gacataagag ctccctgtca gcggcagagc cgtaatgcag ccctgccaga
    38341 gtggatcaac gcgctaaagc tgcggtcagt gccggaagga tcttcacggc atcgccaaca
    38401 atgccgtaat ccgcgtactg gaagatcggc gcatttttat ctttattgat ggcgaaaatg
    38461 gtttgcgacg cgttagcgcc aaccatgtgc tggatctacc cggagatccc caccgccagg
    38521 tacagttcag gtttcagcat caggttggag ataccgacat agcgttcgtg ctccatccat
    38581 ttttcgtttt ctgccaccgg acgagaacag gccaactccg cacctatcgc cttgcaaagc
    38641 tgttctgcca gcgcaatgtt ctctttgctg ccaatgccgc gaccgacgct gaccaccaga
    38701 cgggctttgt cgagatcgac gctgttgctc tggcgcgcct gggttgccgt gcgggtaatc
    38761 gccacagccg gagcctgcca ctccacggtg tgcgtttcgc cagtgcgtga cgcgtctggc
    38821 tgagccgcat cgaacgtgcc gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt
    38881 tcttcgccaa tcgccagacc gccgtaaacc atgtgtttca ctgttgcttt accgtcctgt
    38941 acgctgacgg tgctggcatc gttagacacc gccgctttaa ggcgataacc cagttttgcc
    39001 gccagtaatt tgccgcgacg ggtgtttggc aacagcacca gaccgtcagc gccgtgctgg
    39061 cgaatagtgt cagccatgac accggcgtaa tcttcgatca tccgatcgtc cggtttgccg
    39121 tttaatttcc agacatgatt agcgccgagc tggattgcct gtgcgccgtc ggcatcattg
    39181 aggacaaagg cattgatttg attagctaaa gcctgcgcac cgttcatcag ttccggcaga
    39241 cgagaggggg tatcgctgaa tacccagact tgagaaaacg tgttcatagc atcccctgta
    39301 attaaatgac tttgcgaaga ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt
    39361 caatcacgat gcgctgacgc tcgcgctgtt tcggcgcggc aacctgttgt tcagaccagg
    39421 cgtccacggc gttaaaacca atatccgccg ccgaccatac ctggactggc tttttcgccg
    39481 cgccgagaat ggctttcatc gaaggaattt gtggggagtt gatatcagtg gaaacagcaa
    39541 caaccgcagg cagcggaatg cttaaggttt cggtttcatc ttccagttcg cgctcaacgg
    39601 tgagggtatc tgccgtcagg gagataattt tgctgacgcc gttaactgcc ggaatattga
    39661 ggatttcgcc caccagcaga ccaacctgct gggcataaag gtcggaagaa ccatcgccac
    39721 agaggatcag atcaaagcct gctttctggg cggctgcagc cagtgcgctc gccgtttgtt
    39781 gcggcagtgc ctgctcgaac tggtcatcaa tcaccacaat cagttcatcc gggccgcgcg
    39841 atagcacatc tttacgccct ttggcgttgg tcagggcttt accgcccaca cttaaggctg
    39901 tcacctgcgc ctctgctgcc tgttgcttta gctggcaagc cgcttcaata gcgttgagat
    39961 cgtattggct tattttggca tcggctttgc tgaagtctaa tgaaccatca gcattattga
    40021 ccgcaatatc ctgttcatca ggcacgcact tatagcaagt aataatcttc attgcatctc
    40081 cagaaatcat gaaggaaacg tcgttcatat cggtggtgtt aattacagat atcacgccct
    40141 ttaatttgga aataaaactt taatcaccaa tattgaaagt cagatgctat ttttttaccc
    40201 acaaaagaat ataacaaact gattaataac caaaattaac gaaaaacgcc ctctgttcac
    40261 tcgaaacaaa aatgtgatac caatcacaga atacagctta ttgaataccc attatgagtt
    40321 agccattaac gcgtccacga ggttaataat aattatatta aatgttaaca aaaataaaac
    40381 aaacgggaac gcaaaataaa tattcgtttt cacagtggaa ttaactcatg aagaatgaaa
    40441 agagaaaaac gggaatagaa ccgaaggttt tctttccgcc attaataatc gtcggcatac
    40501 tttgttggct tacagtcaga gatctggatg cggcgaatgt cgttattaat gctgtattca
    40561 gttacgtcac caatgtatgg ggatgggcat ttgaatggta tatggtggtg atgcttttcg
    40621 gttggttctg gctggtgttt ggcccgtatg ccaaaaagcg tttaggtaac gaaccgccag
    40681 aatttagcac cgccagttgg atctttatga tgttcgcctc ctgtacgtct gctgccgtac
    40741 tgttctgggg atcgattgag atctactact acatctccac cccgccgttt ggcttagaac
    40801 cgaactcgac aggagcgaaa gagttggggc tggcttacag cttgttccac tggggacctc
    40861 tgccgtgggc cacttacagc ttcctttcag tcgccttcgc ttacttcttc tttgtccgca
    40921 aaatggaagt gattcgcccc agctcgacac tggtgccgct ggcaggtgaa aaacacgcca
    40981 aagggttgtt cggtaccatc gtcgacaact tctatctcgt cgccttgatc ttcgcgatgg
    41041 gtaccagtct gggccttgcc acgccgctgg tgaccgagtg tatgcaatgg ttgtttggca
    41101 ttccgcatac cctgcaactg gacgctatca tcattacctg ctggattatc ctcaacgcca
    41161 tttgcgtcgc ttgcggtctg caaaaagggg tacgtatcgc cagtgacgtg cgtagttacc
    41221 tgagcttcct gatgctgggt tgggtgttca tcgtcagcgg tgccagcttc atcatgaact
    41281 acttcaccga ttcggtgggg atgttgctga tgtatctgcc gcgcatgttg ttctataccg
    41341 atcccatcgc taaaggcggc ttcccgcagg gctggaccgt gttctactgg gcatggtggg
    41401 tgatttacgc catccagatg agtatcttcc tcgcccgcat ctcccgtggt cgtactgtgc
    41461 gtgaactgtg cttcggcatg gtgatggggc tgacagcatc cacctggatc ctgtggaccg
    41521 tgctcggtag taacactctg ctgttgatgg ataaaaacat catcaacatt ccaaatctga
    41581 tcgaacagta cggtgtggcg cgcgccatca tcgaaacctg ggccgctctg ccactcagca
    41641 ccgccaccat gtggggcttc ttcatcctct gctttattgc caccgttacg ctggttaacg
    41701 cctgctctta taccctggcg atgtctactt gccgcgaagt acgcgatggt gaagaaccac
    41761 cgctgctggt gcgtatcggt tggtcaattc tggttggcat tatcggtatt gttctgctgg
    41821 cgctcggcgg cctgaaaccg attcaaaccg ccattatcgc cggaggatgc ccgctgttct
    41881 tcgtcaacat tatggtgacg ctctccttta ttaaagacgc gaaacagaac tggaaagatt
    41941 aattaacccc caaaatatca agaggttgaa agatggattt taatttaaat gatgagcagg
    42001 aactgtttgt cgccggtatc cgcgaactga tggccagcga aaactgggag gcctattttg
    42061 ccgagtgcga ccgtgacagc gtctacccgg aacgttttgt caaagcactg gcggatatgg
    42121 gtatcgacag tctgctgatc cctgaagagc acggtggtct ggacgcgggg tttgttactc
    42181 tcgccgccgt gtggatggag ctgggacgtc tgggggcacc aacctatgta ctgtaccagc
    42241 tgccgggcgg gttcaatacc ttcctgcgcg aaggcacaca agagcagatc gacaagatta
    42301 tggctttccg cggcaccggt aagcagatgt ggaactcagc gattaccgaa ccgggcgcgg
    42361 gctccgacgt gggtagcctg aaaacgactt atacccgtag aaatggtaag atttatctta
    42421 atggtagtaa gtgttttatt accagtagcg cctacacccc gtacatcgtg gtgatggcgc
    42481 gcgacggggc ttctccggac aaacctgtct acaccgaatg gtttgttgat atgagcaaac
    42541 cgggcatcaa agtgaccaaa cttgagaagc tcggtctgcg tatggatagc tgctgtgaaa
    42601 tcacctttga cgatgtggaa ctggacgaga aagacatgtt cggtcgggaa ggtaacggct
    42661 ttaaccgcgt caaagaagag ttcgaccatg aacgtttcct ggtagccctg accaactacg
    42721 gtacggcgat gtgcgccttt gaagatgcgg cgcgctacgc caaccagcgc gtgcagtttg
    42781 gcgaggctat tggtcgtttc cagttgattc aggaaaaatt cgcccacatg gcgatcaaat
    42841 taaactccat gaaaaacatg ctgtatgaag cagcgtggaa agcagacaac ggcaccatca
    42901 cctctggcga tgcagcgatg tgcaaatact tctgcgccaa tgcggcattt gaagtggtgg
    42961 atagcgcaat gcaggtgctg ggcggtgtcg ggattgcggg caaccaccgc atcagccgct
    43021 tctggcgtga cctgcgtgta gaccgcgttt ccggaggatc tgacgaaatg cagatcctga
    43081 cgctgggtcg tgcggtgctg aagcaatacc gctaagtccg tgcgctgcct gatgcgacgc
    43141 tgacgcgtct tatcaggcct acgggagcac gaatgtaagc cggataaggc gtttacgccg
    43201 catccggcag tcatgcgcta cataaatttt tcaggagcta ataatggatc atctacccat
    43261 gccgaaattc ggaccgctgg ccggattgcg cgttgtcttc tccggtatcg aaatcgccgg
    43321 accgtttgcc ggacaaatgt tcgcagaatg gggcgcggaa gttatttgga tcgagaacgt
    43381 cgcctgggcc gacaccattc gcgttcaacc gaactacccg cagctctccc gccgcaattt
    43441 gcacgcgctg tcgttaaata ttttcaaaga tgaaggccgc gaagcgtttc tgaaattaat
    43501 ggaaaccacc gatatcttca tcgaagccag taaaggtccg gcctttgccc gtcgtggcat
    43561 taccgatgaa gtactgtggc agcacaaccc gaaactggtt atcgctcacc tgtccggttt
    43621 tggtcagtac ggcaccgagg agtacaccaa tcttccggcc tataacacca tcgcccaggc
    43681 cttcagtggt tacctgattc agaacggtga tgttgaccag ccaatgcctg ccttcccgta
    43741 taccgccgat tacttttctg gcctgaccgc caccacggcg gcgctggcag cactgcataa
    43801 agtgcgtgaa accggtaaag gcgaaagtat cgacatcgcc atgtatgaag tgatgctgcg
    43861 tatgggccag tacttcatga tggattactt caacggcggc gaaatgtgcc cgcgcatgag
    43921 caaaggtaaa gatccctact acgccggttg cggtctgtat aaatgtgccg acggctacat
    43981 cgtgatggaa ctggtgggca ttacccaaat tgaagagtgc tttaaagata ttggcctcgc
    44041 acatctactc ggtacgccgg aaatcccgga aggcactcag cttatccacc gtatcgaatg
    44101 cccttacggc ccactggtag aagaaaaact cgatgcctgg ctggcggcac ataccatcgc
    44161 agaagttaaa gaacgctttg ccgaactgaa tatcgcctgt gccaaagtac tgaccgtacc
    44221 ggaactggaa agcaatccac agtatgttgc ccgtgaatca atcactcagt ggcaaacgat
    44281 ggatggtcgc acctgcaaag ggccgaacat catgccgaaa ttcaaaaata accccggaca
    44341 aatctggcgc ggaatgccct cacatggcat ggacacggct gccattttga aaaatatcgg
    44401 ctacagcgaa aacgacattc aggagttggt cagcaaaggt ctggccaaag ttgaggacta
    44461 aacgcgtggc gtcagtcggc ttgcctgact agcgcccgcc caataacaaa cgaacaaaaa
    44521 tggatagagg tgcaatggat atcattggcg gacaacatct acgtcaaatg tgggacgatc
    44581 tggcggacgt ttacggtcat aaaacggcgc tgatttgtga atccagcggc ggagtcgtta
    44641 accggtatag ttatcttgag ttaaatcagg agattaaccg cacggcaaac ctgttttata
    44701 cgctggggat tcgcaaaggc aacaaggttg cactacatct cgacaactgc ccggaattta
    44761 tcttttgctg gttcgggctg gcaaaaattg gcgcgattat ggtgccgatt aacgcccgcc
    44821 tgttacgcga ggaaagcgag tggatcttgc aaaatagcca ggcgtgcctg ctggtgacca
    44881 gtgcgcaatt ttatcctatg tatcaacaga ttcagcagga agatgccact caattgcggc
    44941 acatttgcct gacagatgtg gcacttcccg ctgatgatgg agtgagttca tttactcaac
    45001 tgaaaaatca acaacctgcc accttgtgct atgcaccgcc gctatcgact gacgatacgg
    45061 cggaaattct cttcacctcc ggcaccacct cccgaccgaa aggtgtggtg attacccatt
    45121 acaacctgcg cttcgctgga tattactccg cctggcagtg tgcactgcgt gacgatgacg
    45181 tctacatgac ggtaatgcct gcgtttcata tcgattgcca gtgtactgcg gcgatggcgg
    45241 cgttttctgc cggggccacc tttgtgctgg tcgagaaata cagcgcccgc gccttctggg
    45301 gacaggcgca gaagtaccgc gccaccatta ccgaatgtat tccgatgatg atccgtacgt
    45361 tgatggtgca gccgccttca gcgaacgatc ggcaacaccg cctgcgggaa gtgatgtttt
    45421 atctcaactt gtcggagcag gaaaaagacg cattttgtga acgcttcggc gttcgcttgc
    45481 tgacgtctta tgggatgacg gaaaccattg tgggcattat cggcgatcgc cctagcgata
    45541 aacgacgctg gccgtcgatt ggtcgggcgg ggttttgcta cgaagcggag atccgcgacg
    45601 atcacaatcg cccgctcccg gcaggtgaga tcggtgaaat ctgtattaaa ggcgtaccag
    45661 ggaaaaccat cttcaaagag tattttctca acccgaaagc cactgcgaaa gtgctggaag
    45721 ccgacggctg gctacatacc ggcgataccg gataccgcga cgaagaaggc tttttttatt
    45781 tcgtcgatcg ccgctgcaat atgatcaaac gcggtggcga gaatgtctcc tgcgtggagc
    45841 tggaaaatat catcgccacc catccgaaaa ttcaggacat cgtggttgtg ggtattaaag
    45901 attcgattcg cgatgaagcc atcaaagcat ttgtggtgct gaatgaaggt gaaacattga
    45961 gcgaagagga attttttcgc ttctgcgaac aaaatatggc gaaatttaaa gtgccctctt
    46021 atctggagat cagaaaagat ctgccacgta attgctcggg gaaaataatt agaaagaatc
    46081 tgaaataaat gaaacgtcag ggaactaccc tgccagctaa taatcacgcc ctaaaacaat
    46141 atgcattttt tgcagggatg ttgtcgtccc taaaaaagca aaaatggaga aaaggaatga
    46201 gtgaatcatt acatctgacc cgcaatggat caattctgga aattaccctt gatcgcccaa
    46261 aagcgaatgc tattgatgca aaaaccagct ttgaaatggg cgaagtattt ctaaatttcc
    46321 gtgacgatcc gcaattacgt gtcgccatta ttaccggtgc cggagagaaa ttcttttccg
    46381 cgggctggga tttaaaagcg gcagcagaag gcgaagcacc ggatgctgac tttggcccgg
    46441 gtggttttgc gggattaacc gaaattttca atctcgacaa accagttatc gcagctgtga
    46501 acggctacgc ctttggcggc ggtttcgaac tggcgctggc ggcagatttt attgtttgtg
    46561 ccgataacgc cagcttcgcc ctgccggaag ccaaactggg catcgttcct gacagcggcg
    46621 gtgtgctgcg tctgccgaag atcctgccgc ctaccatcgt caatgaaatg gtgatgaccg
    46681 gcagacgaat gggcgcagaa gaggcgctgc gttgggggat agtcaaccgc gtggttagcc
    46741 aggcggaact gatggataac gcccgcgaac tggctcagca gctggttaac agcgccccgc
    46801 tggcgattgc ggcgctgaaa gagatctacc gcaccaccag cgaaatgccg gtagaagaag
    46861 cgtatcgcta tattcgcagc ggcgtgttga aacactatcc atcggttctg cattcggaag
    46921 atgccattga agggccgctg gcgtttgccg agaagcgcga tccggtgtgg aaaggacgtt
    46981 aacaaccatg agctattacg cctttgaggg gttaattccg gtggttcacc cgacggcgtt
    47041 tgtccatccc agtgccgtct tgattggcga tgtgattgtg ggagccggtg tctacatcgg
    47101 cccactcgcc tcactgcgtg gtgactacgg gcggttgatc gtgcaagcgg gagccaatat
    47161 tcaggatggc tgcattatgc atggctactg cgacactgac accatcgttg gggaaaacgg
    47221 ccatatcggg catggagcga tcctgcatgg ttgtgtgatt ggtcgcgatg cattggtcgg
    47281 gatgaacagc gtaattatgg atggcgcggt cattggcgaa gagagcattg ttgccgcaat
    47341 gagctttgtc aaagcaggct ttagtggcga gaaacgccag ttgttgatgg gtacgccagc
    47401 ccgcgctgta cgcagtgtta gtgacgacga gttacactgg aaacggttga ataccaaaga
    47461 gtatcaggat cttgtcgggc gctgtcatgc atcgttacat gaaacgcagc cactgaggca
    47521 aatggaggaa aatcgccccc gtttgcaggg gacgacggat gtgacgccta aacggtgatc
    47581 ttgccggatg cgacctggtt gctggataag atgctacaac atcgtatctg gcaagcagga
    47641 cgcccggtat caacgagata aaattaacga cacatactct ttgacaacat catctgccac
    47701 ttctgctcgc ggttaagttc cgtgagcgga acggcaggaa tacgcggtgt ctttcttacg
    47761 ccgactaatt tttcccgact gttattgcgc aggcgcgcgt aaatttgctc atcgatatcg
    47821 accactttaa ccagtcgctg acactggcat ccccgccctt ccagcttatt agggatcatc
    47881 ttaacttcgc agcttatttc tgtgacttcc gacagaatat aagtcagggt attaaccgcc
    47941 ttgctgtgtt caatatcgaa atgaatagag atctctcttg ctatcaccca ccgattttca
    48001 gccatcatcc attcggcgat taacaagtag agtggttttt caacatatcc ttcacacata
    48061 cgaactccag tttcacaaat taaaagtcta gctatgactc gttttgaagc aattaaacaa
    48121 ggccatatta aaattgtgga tatatcgata gtatgcaact tcaccgtcga taagtgtgag
    48181 ctaaatcctg cttatgtcat caaaaatatc gattccccaa aagatttact taatgggcaa
    48241 aaaaaaacgg tcctcatcag agaaccgtat tagttattcg atcaaattag cggatgaaaa
    48301 atatccgcca cgacacgcta ttatttgatc tgtgcgtgca tttcctgcac cgaaattact
    48361 ttttcagtcg catcggcatt cagcgccatc gcggtagcga aaccaccgtt cagggtggtg
    48421 tcataatgca ctttatattg cagcgcactg cgacggatca cgcgggagtc ttcaatcgca
    48481 cgacggcctg aggtggtgtt gatgatgtag gtatattcgc cattcttgat acggtcctga
    48541 atgtgcggac ggccttcatg caccttgttt accagacgcg gattgatacc cgcttcgccc
    48601 agcacaatcg ccgtgccgtg ggtcgcatcc agctcgaagc cctgtttcag cagttttgcc
    48661 gccaggtcca ccacgcgttc tttatcgcct tcgcgcacgg aaagcagcgc acgaccgtgt
    48721 ttcttcatgg tggagttgct gcccagctgc gctttggcga acgcttcagc gaaggtgcgg
    48781 cccacgccca tgacttcccc ggtagagcgc atttctggcc ctaacagcgg gtcaacgcct
    48841 gggaatttat tgaacggcag caccacttct ttcaccgagt agtacggcgg aataacttct
    48901 ttggttacgc cctgctcagc cagcgatttg ccagccatca cacgcgccgc cacttttgcc
    48961 agcggtacgc cggtggcttt ggagacgaac ggaacagtac gcgccgcacg cgggtttact
    49021 tcaatcaggt aaacttcgtt gtttttcacc gcaaactgca cgttcatcag accgcgcacc
    49081 tgcaattcga aggccagttt ctgcacctgc tggcgcatca catcctgaat ttcctgactt
    49141 aaggtgtacg ctggcagcga acatgcggag tcaccggagt gcacacccgc ctgctcgatg
    49201 tgctccatga tgccgccaat cagcaccatt tcgccgtcgc agatggcatc cacgtcaact
    49261 tctactgcgt catcaaggaa atgatccagc aacactggcg catcgttaga cacgctaacc
    49321 gccgtctgga agtagcgacg caggtcagct tcgtcataga cgatttccat cgcccgaccg
    49381 ccgagaacgt aagacggacg caccaccagc gggtagccaa tctcttttgc cttctcaact
    49441 gccatttcaa tagcagtaac ggtggcgttc gccggttgtt tcagtttcag acggtcaacc
    49501 gcatgctgga agcgttcacg gtcttccgca cggtcgatag catccgggct ggtgccgata
    49561 accggtacgc cagcggcttc cagtgcacgc gccagtttca gcggggtctg accgccgtac
    49621 tgaacgataa cgcctttcgg cttctcaata cgcacgattt ccagcacatc ttccagagtt
    49681 accggctcga agtagaggcg atcggaagtg tcgtagtcgg tagagacggt ttccgggtta
    49741 cagttaacca taatggtttc gtaaccgtct tcacgcagcg ccagcgaggc gtgtacgcag
    49801 cagtagtcga attcgatacc ctgaccgata cggtttggac cgccgccaag caccatgatt
    49861 ttttcacggt cggtagacgg attcgcttcg cactcttctt cataagtgga gtacatgtaa
    49921 gcggtgtcgg tggcaaactc tgccgcacag gtatccacgc gcttgtagac cgggtgcagg
    49981 tcatattggt cacgcagctt acggatttcc gcttcgcgta cgcccgccag ttttgccaag
    50041 cgcgcatcgg caaagccttt gcgtttcagc tggcgcagga attcagcgtt caggccagtg
    50101 atgcccactt ccgccacttt ctcttccaga cgcaccagtt cttcaatctg taccaggaac
    50161 cagcggtcaa tgttagtcag gttgaagacg ccgtccacag acaggcccgc gcggaaagca
    50221 tcggcgatgt accagatacg ctctgcgcct gcgtctttca gttcgcgacg gattttggtt
    50281 aacgcttccg ggtcatccag gctcactttc gggtcgaagc cagttgcgcc cacttccagg
    50341 ccgcgcagcg ctttttgcag ggattcctgc tgcgtgcgac caatcgccat cacttcgcca
    50401 accgatttca tctgagtggt cagacggtcg ttagcaccgg cgaatttttc gaagttgaag
    50461 cgaggaattt tagtaaccac atagtcgatg gacggctcga aggaggccgg agtacgtccg
    50521 ccagtgatgt cgttcatcag ttcgtctagg gtgtaaccca ccgccagttt cgccgccact
    50581 ttagcaatcg ggaaaccggt cgctttcgac gccagcgccg aagaacggga cacgcgtggg
    50641 ttcatttcga taacaatcag gcgaccgttt ttcgggttca ccgcaaactg gacattggaa
    50701 ccaccggttt caacgccgat ttcacgcagc accgccatcg aggcgttacg catgatttga
    50761 tattctttgt cggtcagcgt ttgggctggc gcgacagtga tggagtcacc ggtgtggatg
    50821 cccatcgcat caaagttttc gatagagcag acgatgatgc agttgtcgtt tttatcacgc
    50881 accacttcca tctcgtactc tttccagccg atcagcgact catcaatcag caactctttg
    50941 gtcggagaga gatccagacc gcgggcgcaa atttcttcaa actcttcacg gttataagcg
    51001 ataccgccgc cgctaccgcc catggtaaag gatgggcgaa taatgcacgg gaagcccacg
    51061 tcagcggcaa cagccagcgc ttcttccatc gtgtgtgcga taccggaacg cgcggtttcc
    51121 agaccaattt tcttcatcgc tacgtcgaaa cgacggcggt cttctgcttt atcaatcgca
    51181 tcggcagtgg caccaatcat ggtgacaccg aactcttcca acacgccctg acgttccagc
    51241 tccagcgcgc agttcagcgc cgtctgaccg cccatggttg gcagcaccgc gtccgggcgc
    51301 tctttttcaa taatcttgcg taccacttcc cagtgaatcg gctcgatgta ggtcgcatcg
    51361 gccatttccg ggtcggtcat gatggtcgcc gggttggagt tcaccagaat gacgcggtaa
    51421 ccctcttcgc gcagggcttt acatgcttgc gcgccagagt agtcaaactc acacgcctga
    51481 ccgataacaa tcgggcccgc acccagaatc aggatacttt ttatatctgt acgttttggc
    51541 atggctcttt tactcctgat tacttagcgg ttttacggta ctgctcaatt aacttgataa
    51601 agtggtcgaa caacggcgcg gcgtcgtgtg gaccagggct ggcttcaggg tgaccctgga
    51661 agctgaacgc cggcttatcg atgcgatgaa tgccctgtaa cgtaccgtcg aacagggatt
    51721 tatgcgtgac acgcaggttt gcaggtaatg ttgcttcatc caccgcaaaa ccgtggttct
    51781 gggcggtgat catcaccacg tttttctcca catctttaac cggatggttg ccgccgtggt
    51841 gaccaaattt cattttgaca gtcttcgcac cgctcgccag cgccagcagc tgatgaccga
    51901 gacagatgcc gaataccgga atatcggttt cgaggaactt ctggatggcg gtaatggcgt
    51961 aatcgcacgg ggccgggtcg ccaggaccgt tggagaggaa gatgccgtct ggattcattt
    52021 tcagcacatc ttccgcagaa gtttgcgccg gaacgatggt caggcgacag cctctatcca
    52081 ccagcatccg caggatgttg cgcttggcac caaaatcata agccacgacg tggaacggca
    52141 gctcgtcttc ttttttcgct tctggcaggc cgccggtcaa cgtccagctc ccttgtgtcc
    52201 agctataggc ttctgcggtg gtcacttctt ttgccagatc catgccgttc agacctggga
    52261 acgcgcgggc tttttctaac gccagcgccg catccgggtt atcgcctgcg ataatgcagc
    52321 cattctgtgc gcctttctcg cgcagtaaac gcgtcagctt acgggtatcg atatcggcaa
    52381 tcgccacgat gttatggcgc ttcaggtaag aagagaggtc ttcggtatta cggaagttgc
    52441 tggcaatcag cggcaggtcg cgaatcacca gaccttgtgc atgtacctga gaagattctt
    52501 catcggcgtc attagtgccg acattgccaa tatggggata agtaagagta acgatttgac
    52561 gagaatagga aggatcagtg aggatttctt gataaccggt cattgaagta ttgaaaacga
    52621 cttccccaac cgccgaacct gttgccccta tggcccgacc gtgaaactgg gttccgtctt
    52681 ccagaaccaa tagcgctgac ttaatcaaaa caccctccag agaatactca ctcactttat
    52741 ttgcatatta attcacagtg atgacgtaaa tcaatgcaaa tctgcttacc gacgaatttc
    52801 tggcaaacgg cggcattctg gagatatggg cgctaaaagt caacttaatg gcaggatttt
    52861 ttatattatt ttgtggctca tgcatgtttt acaggcatta agcgacaaaa acatcatcta
    52921 acctgttctg gaaaacgctt gcgcagcaag aagattgcat gaaataaaaa cgagcaggaa
    52981 aaagtggacc aaatggtcaa
//
LOCUS       DAZXUK010000004        51555 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZXUK010000004 DAZXUK010000000
VERSION     DAZXUK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51555
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.004"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(141..2588)
                     /gene="glgP"
                     /locus_tag="ACH3L8_000164"
     CDS             complement(141..2588)
                     /gene="glgP"
                     /locus_tag="ACH3L8_000164"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFT4083460.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2607..4040)
                     /gene="glgA"
                     /locus_tag="ACH3L8_000165"
     CDS             complement(2607..4040)
                     /gene="glgA"
                     /locus_tag="ACH3L8_000165"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFT4083461.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(4040..5335)
                     /gene="glgC"
                     /locus_tag="ACH3L8_000166"
     CDS             complement(4040..5335)
                     /gene="glgC"
                     /locus_tag="ACH3L8_000166"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFT4083462.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5353..7326)
                     /gene="glgX"
                     /locus_tag="ACH3L8_000167"
     CDS             complement(5353..7326)
                     /gene="glgX"
                     /locus_tag="ACH3L8_000167"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFT4083463.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7323..9509)
                     /gene="glgB"
                     /locus_tag="ACH3L8_000168"
     CDS             complement(7323..9509)
                     /gene="glgB"
                     /locus_tag="ACH3L8_000168"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFT4083464.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9782..10885)
                     /gene="asd"
                     /locus_tag="ACH3L8_000169"
     CDS             complement(9782..10885)
                     /gene="asd"
                     /locus_tag="ACH3L8_000169"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFT4083465.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            11077..11670
                     /gene="yhgN"
                     /locus_tag="ACH3L8_000170"
     CDS             11077..11670
                     /gene="yhgN"
                     /locus_tag="ACH3L8_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFT4083466.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11727..13067)
                     /gene="gntU"
                     /locus_tag="ACH3L8_000171"
     CDS             complement(11727..13067)
                     /gene="gntU"
                     /locus_tag="ACH3L8_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFT4083467.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(13071..13598)
                     /gene="gntK"
                     /locus_tag="ACH3L8_000172"
     CDS             complement(13071..13598)
                     /gene="gntK"
                     /locus_tag="ACH3L8_000172"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFT4083468.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13737..14732)
                     /gene="gntR"
                     /locus_tag="ACH3L8_000173"
     CDS             complement(13737..14732)
                     /gene="gntR"
                     /locus_tag="ACH3L8_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFT4083469.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14956..15651)
                     /gene="yhhW"
                     /locus_tag="ACH3L8_000174"
     CDS             complement(14956..15651)
                     /gene="yhhW"
                     /locus_tag="ACH3L8_000174"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFT4083470.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15774..16811)
                     /locus_tag="ACH3L8_000175"
     CDS             complement(15774..16811)
                     /locus_tag="ACH3L8_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFT4083471.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17144..17632
                     /gene="yhhY"
                     /locus_tag="ACH3L8_000176"
     CDS             17144..17632
                     /gene="yhhY"
                     /locus_tag="ACH3L8_000176"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFT4083472.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17691..19433)
                     /gene="ggt"
                     /locus_tag="ACH3L8_000177"
     CDS             complement(17691..19433)
                     /gene="ggt"
                     /locus_tag="ACH3L8_000177"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFT4083473.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19553..19993
                     /locus_tag="ACH3L8_000178"
     CDS             19553..19993
                     /locus_tag="ACH3L8_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFT4083474.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19980..20723)
                     /gene="ugpQ"
                     /locus_tag="ACH3L8_000179"
     CDS             complement(19980..20723)
                     /gene="ugpQ"
                     /locus_tag="ACH3L8_000179"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFT4083475.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20720..21653)
                     /locus_tag="ACH3L8_000180"
                     /pseudo
     CDS             complement(20720..21653)
                     /locus_tag="ACH3L8_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21712..22557)
                     /gene="ugpE"
                     /locus_tag="ACH3L8_000181"
     CDS             complement(21712..22557)
                     /gene="ugpE"
                     /locus_tag="ACH3L8_000181"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFT4083476.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22554..23441)
                     /gene="ugpA"
                     /locus_tag="ACH3L8_000182"
     CDS             complement(22554..23441)
                     /gene="ugpA"
                     /locus_tag="ACH3L8_000182"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFT4083477.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23539..24855)
                     /gene="ugpB"
                     /locus_tag="ACH3L8_000183"
     CDS             complement(23539..24855)
                     /gene="ugpB"
                     /locus_tag="ACH3L8_000183"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFT4083478.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25252..25965)
                     /gene="livF"
                     /locus_tag="ACH3L8_000184"
     CDS             complement(25252..25965)
                     /gene="livF"
                     /locus_tag="ACH3L8_000184"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFT4083479.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25967..26734)
                     /gene="livG"
                     /locus_tag="ACH3L8_000185"
     CDS             complement(25967..26734)
                     /gene="livG"
                     /locus_tag="ACH3L8_000185"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFT4083480.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26731..28008)
                     /gene="livM"
                     /locus_tag="ACH3L8_000186"
     CDS             complement(26731..28008)
                     /gene="livM"
                     /locus_tag="ACH3L8_000186"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFT4083481.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRTQLKLKNGAAKGEQA"
     gene            complement(28005..28931)
                     /gene="livH"
                     /locus_tag="ACH3L8_000187"
     CDS             complement(28005..28931)
                     /gene="livH"
                     /locus_tag="ACH3L8_000187"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFT4083482.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28979..30088)
                     /gene="livK"
                     /locus_tag="ACH3L8_000188"
     CDS             complement(28979..30088)
                     /gene="livK"
                     /locus_tag="ACH3L8_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFT4083483.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30512..30895
                     /gene="panM"
                     /locus_tag="ACH3L8_000189"
     CDS             30512..30895
                     /gene="panM"
                     /locus_tag="ACH3L8_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFT4083484.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(31094..32197)
                     /gene="livJ"
                     /locus_tag="ACH3L8_000190"
     CDS             complement(31094..32197)
                     /gene="livJ"
                     /locus_tag="ACH3L8_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFT4083485.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32468..33322)
                     /gene="rpoH"
                     /locus_tag="ACH3L8_000191"
     CDS             complement(32468..33322)
                     /gene="rpoH"
                     /locus_tag="ACH3L8_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFT4083486.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33567..34625)
                     /gene="ftsX"
                     /locus_tag="ACH3L8_000192"
     CDS             complement(33567..34625)
                     /gene="ftsX"
                     /locus_tag="ACH3L8_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFT4083487.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVTQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34618..35286)
                     /gene="ftsE"
                     /locus_tag="ACH3L8_000193"
     CDS             complement(34618..35286)
                     /gene="ftsE"
                     /locus_tag="ACH3L8_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFT4083488.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35289..36785)
                     /gene="ftsY"
                     /locus_tag="ACH3L8_000194"
     CDS             complement(35289..36785)
                     /gene="ftsY"
                     /locus_tag="ACH3L8_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFT4083489.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36935..37531
                     /gene="rsmD"
                     /locus_tag="ACH3L8_000195"
     CDS             36935..37531
                     /gene="rsmD"
                     /locus_tag="ACH3L8_000195"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFT4083490.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37521..37790
                     /locus_tag="ACH3L8_000196"
     CDS             37521..37790
                     /locus_tag="ACH3L8_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFT4083491.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37793..38152)
                     /locus_tag="ACH3L8_000197"
     CDS             complement(37793..38152)
                     /locus_tag="ACH3L8_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFT4083492.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38293..38919
                     /locus_tag="ACH3L8_000198"
     CDS             38293..38919
                     /locus_tag="ACH3L8_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFT4083493.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38993..41191
                     /gene="zntA"
                     /locus_tag="ACH3L8_000199"
     CDS             38993..41191
                     /gene="zntA"
                     /locus_tag="ACH3L8_000199"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFT4083494.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41293..41538)
                     /gene="tusA"
                     /locus_tag="ACH3L8_000200"
     CDS             complement(41293..41538)
                     /gene="tusA"
                     /locus_tag="ACH3L8_000200"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFT4083495.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41759..42424
                     /locus_tag="ACH3L8_000201"
     CDS             41759..42424
                     /locus_tag="ACH3L8_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFT4083496.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42497..43054
                     /gene="dcrB"
                     /locus_tag="ACH3L8_000202"
     CDS             42497..43054
                     /gene="dcrB"
                     /locus_tag="ACH3L8_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFT4083497.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(43058..44275)
                     /locus_tag="ACH3L8_000203"
     CDS             complement(43058..44275)
                     /locus_tag="ACH3L8_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4083498.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44409..45458
                     /locus_tag="ACH3L8_000204"
     CDS             44409..45458
                     /locus_tag="ACH3L8_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFT4083499.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45513..46100
                     /gene="acpT"
                     /locus_tag="ACH3L8_000205"
     CDS             45513..46100
                     /gene="acpT"
                     /locus_tag="ACH3L8_000205"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFT4083500.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46211..47785
                     /gene="nikA"
                     /locus_tag="ACH3L8_000206"
     CDS             46211..47785
                     /gene="nikA"
                     /locus_tag="ACH3L8_000206"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFT4083501.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47785..48729
                     /gene="nikB"
                     /locus_tag="ACH3L8_000207"
     CDS             47785..48729
                     /gene="nikB"
                     /locus_tag="ACH3L8_000207"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFT4083502.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48726..49559
                     /gene="nikC"
                     /locus_tag="ACH3L8_000208"
     CDS             48726..49559
                     /gene="nikC"
                     /locus_tag="ACH3L8_000208"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFT4083503.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49559..50323
                     /gene="nikD"
                     /locus_tag="ACH3L8_000209"
     CDS             49559..50323
                     /gene="nikD"
                     /locus_tag="ACH3L8_000209"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFT4083504.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50320..51126
                     /gene="nikE"
                     /locus_tag="ACH3L8_000210"
     CDS             50320..51126
                     /gene="nikE"
                     /locus_tag="ACH3L8_000210"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFT4083505.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51132..51533
                     /gene="nikR"
                     /locus_tag="ACH3L8_000211"
     CDS             51132..51533
                     /gene="nikR"
                     /locus_tag="ACH3L8_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFT4083506.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
BASE COUNT        12080 a        14312 c        13225 g        11938 t
ORIGIN      
        1 tcaagagcct gtccaatgtg cataaaaaaa tccggaaaca agtgagcgtt tccggattct
       61 tacacagcca ctggatcggt caactgatcc ttaactgatc ggcattagac cctttggccc
      121 cgttctattt attagtgaaa ttacaatctc accggatcga tatgccagat atgatcagcg
      181 tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt cagcatcgct
      241 tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg acaatcgaca
      301 tagctgcgat aatccgccag tacctggtag tgatcgccga agttgatcag cgaatcaacc
      361 agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat ttgcgtcagc
      421 acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc ctgacgacgc
      481 agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc accgacatga
      541 tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc gttaagcgca
      601 aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg ttcagacaga
      661 tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat gaacacgact
      721 ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc attgatcaaa
      781 tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa aatattcacg
      841 cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt aatcacatgc
      901 aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac atcgaacaac
      961 gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag acgctttttg
     1021 ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca gtgttgttgc
     1081 agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc gtccagcacg
     1141 gctgaaagcg atgggttcgc taccgccagc cagcgacgcg gcgtcacacc gttggtgacg
     1201 ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg caccatcaga
     1261 ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc cagccaggcc
     1321 atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag cagatcggta
     1381 tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc aaagatgatc
     1441 tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc cagcgcctcg
     1501 ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa cgcgtcgtcc
     1561 cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga cagtaccgga
     1621 tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt tttatgcaac
     1681 tgataatgac ggcttaaaat gtcctgaatg gtcgaggaaa ccaagaagta ttcctgacgc
     1741 aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg agatacgttc
     1801 tcggagtggt ttttatcttc cactgccgcg aagtagtcac cctggttgaa tttaccgagg
     1861 ttaatttcgc tactggcttg cgcactccac aaacgcagcg tgttggtcgc gtcggtgtcg
     1921 taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc aatccagcgc
     1981 gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg cgtgttgtgg
     2041 cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc tttctggcta
     2101 ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta accgcgcccc
     2161 ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc caggccaccg
     2221 ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa ccccatcgct
     2281 tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt ggagagcgta
     2341 cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg cgacaactgg
     2401 gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa taacgttgcg
     2461 ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa catcagcttg
     2521 taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata tgtaaacgga
     2581 gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc acggtacgac
     2641 ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac aaaccgccac
     2701 agtgaaggac gggaccacag tacaaaagca cgccgaatag cccgtaacag cgaccaggca
     2761 ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc aagagaacag
     2821 tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt accgtacttc
     2881 aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag aatgacgtcc
     2941 gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac gcccacctga
     3001 ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc gcccgcgccg
     3061 agtagcgcca gctgcccgcc ctgctccaga agccccggta aggcttccag caccaggtcg
     3121 agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt atcgtcaacc
     3181 ttaagcccca ttgcgatttg taactggctc ttattttccg ctttatcttc caacgtatcg
     3241 cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt ctcgtccacg
     3301 ccgttcagta cgcctgaaag acgcccttca cggtgacgct gttgcaacag accttccata
     3361 ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac cgccgtaatg
     3421 tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa ttccagccca
     3481 tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc ataaaacatg
     3541 ccttgatagg ccaggttgtg cacagtaaac accgacttcg caggacgccc gcgcgccgcc
     3601 agatacgcag gcgcaagacc tgcatgccag tcgtgcacat gcaccacatc aggacgccag
     3661 aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc aaaacgcaat
     3721 acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg acgatcatag
     3781 agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa caacagcgtg
     3841 atatgcccgg cgaaagtatc acgacgggat actacctgcg catcggtcac gccacggcga
     3901 atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat ttgtgctgcg
     3961 ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa catctctgaa
     4021 catacatgta aaacctgcat tatcgctcct gtttatgccc taacttccgt agcatttcgc
     4081 gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct tcctctgcgt
     4141 tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg cagcggcgca
     4201 gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca atgttgcaga
     4261 atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa ccggagatca
     4321 cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag cgatcctgca
     4381 cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg cgatcgtaca
     4441 tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa gcttccagcg
     4501 tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa gagagcggga
     4561 acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct ttgccaaagt
     4621 cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga tagtcggcgt
     4681 caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc attgacggtg
     4741 ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc gccataacgc
     4801 caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa cgtgcgcctt
     4861 tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg tcgcccgcca
     4921 ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg gtgaccgcat
     4981 ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc agcagatcga
     5041 caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc tgcaccagag
     5101 tgtgggactg gtactgggtg atcacgccca tacgacggat cccagagttg atgcagttag
     5161 acagcgcaaa gtcaataatg cggaacttac cgccgaagtg tacggccggt tttgctcgct
     5221 tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg gcaacagatt
     5281 tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta accatgacta
     5341 actcctcttt tatcatctct ggaacacaca caatccgtgt gcaggtccct gccagacaac
     5401 cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact ccccagcagg
     5461 taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc gatccgagag
     5521 cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag gttgagcata
     5581 tcgatttagc caacggacat tgccgtcacc ttcttcccac cagcgattct ccaccaaagc
     5641 gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac cactgcttgc
     5701 ctgcgaccag tccaaccagt ttaattggtt atcctgacag taggcattgt tattgccatg
     5761 ctggctgtga ccatgttcgt caccggccag taacatcggc gtgccctggg agagcaacaa
     5821 cgttgttaac agggcatgaa tgctgtcgcg ccgccgttca accaggtcaa gagtaccgcc
     5881 taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc gattttcttc
     5941 tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg taaaaccgtc
     6001 atgcgcggtg acgagattaa tcgcggcact cggcagacga ccattacgtt taaaaacatc
     6061 gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat gtagccagaa
     6121 acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg ggaaatttcc
     6181 cacctgataa ccactaggag cgatatccca cggttcagca attaacttca cctgcgagag
     6241 caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact ctggcgtacg
     6301 gcccatgact gccgccagat caaagcggaa accatcgacg tggcaggttt ctacccaata
     6361 acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga gcgtgttgcc
     6421 gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc tacggttatc
     6481 gatcccacgc agcgagaata acgggccgtc gaggtccagt tccgcactat ggttgagcac
     6541 gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat cgcgaaactc
     6601 atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca tcgccaccgg
     6661 gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac tggcaaactg
     6721 cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt tgatcatcac
     6781 cggatgcccg agggctttat aagtgccacg gatctcgacc gggatctccg ggtgcaagta
     6841 cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg tgcgcggcgg
     6901 ggcgtcatct tcccagtcat agtgatcaac caccactacg catttcggcg caatggcggc
     6961 gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat ctttaaactc
     7021 cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa agcgatgccc
     7081 ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg ggcgcgcatc
     7141 cggcagataa ccgtgccaaa tgtcgccact gttccctggc aagtcatagc gatgttcctg
     7201 gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga aaagtgtgaa
     7261 gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa tggcgagttg
     7321 tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagcgt caggcttagt
     7381 gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc attgcctgca
     7441 ttactgccgt gatagtgtat ggaatcggta ttgaggattt cacgccattt acctggctga
     7501 ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc aacgataatt
     7561 tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt gtcatccacc
     7621 accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt atggtggcgg
     7681 taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt atcgccgcct
     7741 tccaacagat gccagtcgag gctggcgtca tggttccact cgcggccctg ggcaaattcg
     7801 ttccccatga acagcagttt cttgcccgga aatgcccaca tccagccata gtaagcgcgc
     7861 aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga ttttttaccg
     7921 tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt gtagagcatc
     7981 ccgaaggtca gtttatcgtg atgatactga cgataaatcg ggtcgagctt catgtagtcc
     8041 agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc gcccatatcc
     8101 tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac cgcaccggaa
     8161 acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc aagattctcg
     8221 cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc acgataaatc
     8281 atcgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat ccagtaaagc
     8341 gcgttaccga cgaggaagtt actgacttca cggcgaccat agttgtagat cagcgtgttc
     8401 cagtcctgat gatagccttc acgcggatcg ctgtgttcat acaagttcgt gccatcaaat
     8461 tcggcaagcg caaagtcatc agtcgggaag tggcctggca cccagtcgag aatcacgttc
     8521 agaccagctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt accaaaacgg
     8581 cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa gggatgctcg
     8641 ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg caccagttga
     8701 tcggccagct cgcggtagct caaccagaaa ttgttgtcgg tgtggcgacg ccaggaaccc
     8761 aggtgaactt cataaataga gattggcgca tcaaactgat tcgctttttt gcgctcttca
     8821 gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg gcgcatttgc
     8881 gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc aatcatctcg
     8941 tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccagat gccgctctct
     9001 ttacgcaggc gcatcgggtg acggcgaccg tcccagtagt tgaattgccc aaccaccgag
     9061 acccgacggg cgtttggagc ccagacagag aaacgcgtac ctgtgacgcc atccatagta
     9121 tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga taatagccag
     9181 gcatccattt cctggattag cggaccaaaa cggtaaggat catcaatcag gttttgctga
     9241 ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg aatgacgccg
     9301 ctaaagaatc cccgtgagtc gagacactcc agttttgcga gtttgcgccc ggttttcggt
     9361 tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag tcccgcggtg
     9421 gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat tagcgcgtta
     9481 atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg tgtcacccca
     9541 tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg cgcagcactc
     9601 tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc atgtattcag
     9661 ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga aacatctgcg
     9721 cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta atgaataaag
     9781 attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac agcagctggt
     9841 cgcccacggt aaaggctgac aggaactctg gtcccatatt cagcttacgc aggcggccta
     9901 ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata gtgatttccc
     9961 gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct tcgacggtcg
    10021 gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag cgcaatgccc
    10081 cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg atcttgttgg
    10141 tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt ttgtcgatcc
    10201 acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc tcaccgctac
    10261 gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg gtcgcgagtt
    10321 catctgccac atggccatac agatggccca tctgggttaa taactcacgc atatgtcgcg
    10381 caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca agatcattgg
    10441 cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg ccaacaaaag
    10501 tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg tcaagaatga
    10561 tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa ccttgccatc
    10621 cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga caggtcacaa
    10681 tgatatcgag ggcctttagc gcctctagat caaaggcatc ctgaagtgtg ccagtggttc
    10741 cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca gggcgaatgg
    10801 cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc ataccgcgcc
    10861 agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg tgctgtataa
    10921 atgtgccggt ctcctcttgg cacatctttc accatacaaa aagctgccaa agtcgcaagt
    10981 gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt tgttagcacg
    11041 cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat ttctgcagca
    11101 gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat gtccgtactg
    11161 aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct tattgctctc
    11221 ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct cagcctgcga
    11281 gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat taagatgatt
    11341 ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc atttatcgtg
    11401 ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat gttgttgtct
    11461 catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc ctggggcggc
    11521 acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga gaaaggggtg
    11581 aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaactca gatgttcctc
    11641 gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa gagcccggta
    11701 gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat catcccaacg
    11761 atggcaccaa cagtgccgag gatggtttcc atcatggtcc aggttttcag cgtttcggct
    11821 tcggtcgcgc cggtaaattt accgaacaac cagaaaccag cgtcgttaac gtggctgaca
    11881 acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc agagtagttc
    11941 agttgttcaa tgactggcat caccagtccc accgccgtta aacaggctac ggtggcagcc
    12001 ccctgaatga tgcgcactgc agcagccagc acgaagcagg tgatagcaat cggcaggccc
    12061 atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag cacctgtttg
    12121 aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgcag cgcgtgaccg
    12181 caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc ataaatcgct
    12241 accagacaag caaccagaat cgcggtaaac ggatgaccaa taaactcgaa ccattcgtaa
    12301 gcggtagagc cttctggcac aaaacgcgcg gcaatggttt tcagccccac cagcaccagc
    12361 ggcaacagga tcagcgacag gctgaatccg aaagatggca ttttaccttc gccgagatgc
    12421 ggttcgctga tgtcgtcagg aatatgcagc tcaacgtaac ggctgatgaa gttaccccac
    12481 agcggcccgg caataatcat tcccggaatt gccgcacaca ggccaatcag gatcatccag
    12541 ccaaagtcgg cattcatctg cgatgccagc agcattggcg ctggcccagg caccaggaac
    12601 gctgccgcgg ccgccacgcc tgcgaataat gggattacca gcttcaccag gttcgtaccg
    12661 gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa caatcgccac ttcaaagaac
    12721 agcggtagcg cacagaccag ccccgcaagg ccgatggcat aatgcgcgcg gctgtgaccg
    12781 aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcata taagatcttg
    12841 ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggtgcc tcccatccct
    12901 ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc agcccccatg
    12961 gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa aaacagcagc
    13021 agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac ttatttgcct
    13081 tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc gatatccacc
    13141 accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg cgtcaccaac
    13201 atttgggttt taaagaaatg gcctttgcgc gctttcaggc gactttcaat cacatcaaaa
    13261 tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa gtcgcgatag
    13321 tgctttttca gtgcagaaca ggcgatcagc gacactttat tggtgcgctg catagcaaac
    13381 gcggagtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag cggttcgcca
    13441 gacgccattt tttcgatatt gcgccgtgga tggaggaaat cgccatcaag aaacgcggca
    13501 tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga tacgcccatc
    13561 aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc acaatacaag
    13621 aattaaaaat gttacgggta actgttatcg gcaacattgt ccagccggac aatatcagaa
    13681 gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt gtgggcttaa
    13741 atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt cacagattcg
    13801 ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc acgcggcgtc
    13861 agcacgctcg caagtcgtgg ctccatcacc tgaccaatgt catgaccgtg gaaaccggca
    13921 atcgccatat cgtcaggaac ttttaacccc agacgctgac attcaaacgc cgcgccgacc
    13981 gccagatcat cattagtaca gaacacgcca tccagctgcg gatattcccg ccgcgcctgg
    14041 cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac gctatatggc
    14101 accaggcctg catccagcat cgcctgttcg tatcccttct gtttgatgat agtacgttcg
    14161 tcgagacgtg cgccgagata ggcaatgtgg cgatgcccac gagcaataat ggcggtagtc
    14221 atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca cggcgactgg
    14281 ctgtccatca actccaccac cgggatcccc gccacttcaa tcatctttaa ggtgcgcggc
    14341 gtgtgggtac gttcggtgag gatcaggccg tcgatattcc aggagagcat ggattcgaga
    14401 cgttcttgct ccatttccgg tttataaccg tagtgcgcca gcatggtctg ataaccgtgc
    14461 gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt ggtgagagaa
    14521 ggtaacagga cgccaatcgc ccggctggta gcgttagaga ggatatcggg cgcacgattg
    14581 ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc gacggaaacc
    14641 tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac acggtcagcc
    14701 acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa gagatagatt
    14761 ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct tgtttacaat
    14821 tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc aaaaccccgg
    14881 aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg caggcttcaa
    14941 agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc tgtcgctatc
    15001 cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg tcgaggcttt
    15061 cacgccgtta atggtaacat tgccttttac cacctgaatc cagacgcggc gttcagcggc
    15121 aatctgatgc accgactgct catctttcag caacgcccaa cgatacagtt ccatatcctg
    15181 atgcactttc agcgaaccat ctcgcgcatc cggcgagagc accagctgtt tgccctgcac
    15241 ggcatcgaag cgacgctgtt cataacgcgg cgtaatgcca ttttcttcag gcatgatcca
    15301 gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt gacgaatacc
    15361 cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat tgcccatgct
    15421 gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat ctttatgcgg
    15481 gtgagtgccg aagccctgcc ctacttcaat cacgtcgtcg ttaatcacgc gcagcgcgga
    15541 gaagcccata aagttcggat cgtaatagtt ggcacaagag aaagtatgcc aggagtccag
    15601 ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca tttgtattcg
    15661 ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat gatagagggt
    15721 gaaaattgcc ccctctgttc aaaaaaattg aactattcga ggggccatca aacttaattc
    15781 gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat ttccagattg
    15841 gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt ttgatacaac
    15901 gcatcataaa cacgcccgta atcgcccatc tccggcttca tctcttctct gaccgtcaca
    15961 ccctcgtcat tgacatactc cagcacaccg accgaatcat ccgctgcgaa tcccggttcg
    16021 cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta tttaataaac
    16081 gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag atggctggtt
    16141 ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc gtcaggattg
    16201 gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc gaacagagaa
    16261 ataatctggt ccatcgtatg cacaccaagg ccatagaacg cgccatcctg cggcagtcca
    16321 ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc gacaatctct
    16381 cccagcttgc cgctttcaat cgcttttttc gccgtcagga agcaggaatc aaagcgacga
    16441 ttctgatatg gcgtgacggt cagccctttg cttttcgcca gtgcaaacag ctctttcgcc
    16501 tgcgcaagtg tcggagtgaa cgttttttcg accagcacat ttttcccggc ttccagcgcg
    16561 cgcttcgcgt actcgaagtg gctgtccgca tgggtgcaga caacaaccaa cttaacatcg
    16621 ggatcgttta gcacttcgtc gagatcgctg gtgaaatgga tatgagagta aatgggagcc
    16681 tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct atccttgcgg
    16741 ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat aaaggcgcag
    16801 ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg caaagcggac
    16861 gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa attgagaacg
    16921 aaagatcaaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc aatgtgagca
    16981 atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgatacaat aataatcatt
    17041 ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc atttgaaagt
    17101 caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg agatagtaat
    17161 acgccacgca gaaacacggg attacgaggc catcaggcag attcacgccc agccggaggt
    17221 gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg agcgactcgc
    17281 cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg tgggccatct
    17341 caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg gtatctgtgt
    17401 cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga tgattgaaat
    17461 gtgcgacaac tggttacggg tagatcgcat tgaactaacc gtgtttgtcg ataacgcgcc
    17521 agcaattaag gtctataaaa aattcggctt tgagattgaa gggaccggta agaagtacgc
    17581 attgcgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaaat agagaatttg
    17641 caatctaagt ttgtcattta agccctcacc taccgggagg gcttaagata ttaatacccc
    17701 gccgttaaat catccaccga gcgcgggtcg gatgcgccgt acaactcacc gtccggccca
    17761 accataatgc tttgtgtgct gcccatcgcc tctttcagcg ccactttctg accttttgct
    17821 tccagcagct tgagcgtatc cgggctaaac cctttttcga cacgcagctc gtccggcaac
    17881 cactgatggt ggaaacgcgg cgcattggtc gcttcggcga cgttcatgcc ataatcgatg
    17941 ctattcacca ccatttgcag caccgtggtg ataatccggc taccgcctgg gctaccggta
    18001 accagccagg ttttaccgtc tttcaccaca atggtcggcg acatcgacga cagcgggcgt
    18061 ttgttcggcc cgacggcgtt ggcatcaccg cccaccagcc cgtaaacgtt cggtacgccc
    18121 ggtttggcgg agaaatcatc catctggtta ttaagcagaa taccgctctc acccgcgaca
    18181 atacccgtac cgaaggtggt gttcagcgta taggtcaccg ccaccgcgtt accgtcttta
    18241 tccactacag agtaatgggt agtttgatta ctctcataag gcgcaagctt gccagggcga
    18301 atctcactgg acggcttcgc ttttttgata tcaatttgat cggcaatgga tttggcatag
    18361 gctttattgg tcagtgcctg ccacgggact ttgacaaaat ccgggtcgcc aagatattcc
    18421 gagcggtcgg cgtaggcgta tttctccgct tctgccatga tttgcatcgc atcggcgctg
    18481 ccaaagccgt atttcttcat atcgaagttt tccagaatat tgaggatttg tacgatatgg
    18541 atcccgccgg aggatggcgg tggcatggag taaacctgat acccgcgata atcgccgctt
    18601 atcggagtgc gttcgaccgc tttataggcc gctaaatctt ctttagtgat caagccaccg
    18661 tttttctgca tctcctgggc gatctgttcc gcaatcgtgc ctttatagaa ttcgtccggg
    18721 ccgttttcag caatcatctc caggctcttt gccaggttcg cctgcaccag cgtgtcgccc
    18781 tttttcagcg gctcgccctc tttccagaag atagctttac tgttttcgtg attcggcaac
    18841 acttcgctac cgtaggtttt gagatcgtca gccagcgcgt cgttaacgat aaaaccatcg
    18901 cgtgccagtt taaacgcggg ctgcacgact ttgttcagcg gcatggtgcc gtatttatcc
    18961 agcgccagcg agaaacctgc taccgtaccc ggtgtgccgg aagccagatg cgaagtgagt
    19021 gattttttgc tgtccgggtt gccctgatca tcgaggaaca tatcgcgggt cgctttggcg
    19081 ggtgccattt cgcggaaatc gatagccgtg gtattgccat ttttcgagcg gattaacata
    19141 aaaccaccac cgcccagatt ccctgcctgc ggatgcgtta ccgccagcgc gtagcccacc
    19201 gccacggcgg catcaacggc attcccgccc tccttgagga tatccacccc cacctgagtg
    19261 gcagtggcgt ccacagacgc taccattccc tgtttcgcgc gtaccggatg gaagacatct
    19321 tcctccacac catacgagac gggcggcgca ggaggcgcgg cggcggcgct aaaacaactt
    19381 cctgagagca gagcagcaat ggccacccgg cgtaaaaacg tcggttttat catcgttatt
    19441 ctccagagat taaggggcaa ccccagctaa gcctggtata taactctgaa ttaatcatcg
    19501 ttttgtcggg aagcgagtaa acttaaagga tatcttcaga ggaggatacg cgatgaaacg
    19561 acttctgatt cttacggcac tcctgccgtt tgtcggcttt gcacagccca ttaatactct
    19621 gaacaaccct aaccagccgg ggtatcagat acccagccag cagcggatgc aaacccagat
    19681 gcagactcag caaatccagc aaaaagggat gctgaatcag caactgaaaa cgcaaactca
    19741 gttgcaacag cagcatttag aaaaccagat aaacaataat tctcagcggg tgttgcagtc
    19801 acagccgggg gagcgaaatc ccgcccggca gcaaatgctg cccaacacca acggcgggat
    19861 gttaaacagc aaccgtaatc cggatagttc gttgaatcag cagcatatgc tgccggagag
    19921 gagaaacggc gacatgctga atcagcccag cacgccgcag cctgacattc cgttgaaaac
    19981 tattgggccg taaagttcgg accaatcacg tcaatcgcat cggtacagat gcaatccacg
    20041 ccccagcgca gcaactctgc cgcgtgctgg ggtttattga cggtataaac cagaatccgt
    20101 aatccggcgt ctttcaactg catcactcgc gctttatcga gtaacttatg attgagatga
    20161 atagagacgc agcccagccg cgcggtcagt tcgcgccagt cgtcgcgcca ctcatcaagc
    20221 aataaaccac gcggcagttc cggtgccgcc tgttgtgcag cttctaaagc atcaatctca
    20281 aacgatgaca gcagcggcgg cgtcataccg gcccacagtt ggcgtgccgc cagcgccacc
    20341 attttgcccg ttaatggtcc agtgccagtg gtgggtttga tttcgatatt cgccatcatc
    20401 ccgtgttcgc gacagcgttc cgccacctgc gaaagcaacg gcagcggctc acctttaaac
    20461 gctttgctgt accaactgcc cgcatccacg cgcagtaaat cctgccagtt cagttcaccc
    20521 gcgacgcccc agccgttgct ggtgcgttcg agattgtcgt catggagcag gaagatctcg
    20581 ccatctttcg ataacttcgc gtcaaattcg atcatcttat gaccgtattt tgccccgacg
    20641 tcgattgccg ccagggtgtt ttccggggcc agcttaccgc cgccacgatg agcgacgatg
    20701 cggggataag gccagttact catactcgtt gtcctgtttc accatcaaaa agatgcagct
    20761 gattttccgc cagatgcagc cacagcgtgc tgcctgccgt cgggcgctcc tgatgcgcca
    20821 gtcgcaccac cagcttctgc ttgcgagctt agcgcaatat gttccgggcg aatgccgagg
    20881 gtcattttac gcccggcata ctgacggtag ccaccgttta gcggtagcac gataccgccg
    20941 tccagttcga aatgcgtgcc ttcattattc atgcggcctg tcagcaggtt catcgccggg
    21001 ctgccgataa aactcgccac aaacaggctg gcgggctttt cgtagacttc caccggcgtg
    21061 ccaatctgtt cggcaacgcc gccgttcatc accattactc gctgggcgag cgtcatcgcc
    21121 tcaacctgat cgtgagtaac gtagagtgaa gtcgttttca ggcgacggtg cagctgttgc
    21181 agttcaagac gcatctgtac gcgcagcttg gcatcgaggt tagagagtgg ctcatcaaac
    21241 aagaacaccg ccggatcgcg cacaatcgcg cgtcccatcg ccacacgctg gcgctgaccg
    21301 ccggaaagct cgcgcgggcg acgtttgagc agaccgtcca gctccagaat gcgcgccgct
    21361 tctttaacgc gctcggctat ttgctgcttg cccatgccgc gaattttcag cccccacgcc
    21421 atgttttctt cgacgctcat atgcggataa agcgcgtaat tctggaacac catcgcaatc
    21481 ccgcgatctt tcggctccat ttcggtcacg cgctggtcgt tgatccagat atcgccttcg
    21541 gtcaccctct ccagcccggc aaccatacgc agcagcgtcg atttcccgca gccagacggc
    21601 ccgaccatca cgataaattc gccatccgcc acatcaaggg tcagcggttt aatgacctag
    21661 gttttgccat cccagctttt ggttactgcc tgtaatttca gtcctgccat cttatttctc
    21721 actatcgacc aggccgcgca cgaaggcacg ctgcatcact aaaacaatca ctactggtgg
    21781 gatcagggtt aacaacatcg ccgccatcac tgagttccat tccgtggtgc cttcgccagt
    21841 ggcgatcatc cctttgatcc ctgccacggt ggtgccgaga tccacgtcgg taataatcaa
    21901 caacggccac aaatactggt tccagccgta aataaaggtg atcacaaaca gcgccgccag
    21961 attagttttg gagagcggaa aaacgatgtc gcaaaagaag cgcattggtg acgcaccgtc
    22021 gatccgcgcc gcttccacca gttcatccgg cagcgtcata aagaactggc gaaacagaaa
    22081 cgtggcggtc gccgaggcca tcagcggcag cgttaaaccg gcgtagctgt cgagcatctt
    22141 caggttggcg atgacttcca ccgtcgggaa gatacgtact tcaaccggca gcatcagggt
    22201 gataaaaatc atccagaaga agaggttacg tagcggaaaa cgaaaccaga caatggcaaa
    22261 tgccgagagc atcgagacag taattttgcc gagcgtaatg ctgaacgcca tcacaaagct
    22321 gttaagcaac atccgccaga acggcgcgct atttgtgcct accccgttca cccagatgtt
    22381 gtggatgttt tccagcagat gcgtgccggg gatgagcgtc atcggcgcgg cgtagacggc
    22441 ctgtttatcc agcgtcgccg cgacaaacgc cacgtacagc gggaagagga tcaccgcgat
    22501 cccgaggatc agcatggtat ggctgaatat cgtcagccac ggacggttct caatcattgg
    22561 taacgcacct tgctttcaac atagcggaac tgcaccaccg tcagcacgat gacgaggaac
    22621 atcagcacca tcgactgtgc ggcagacgaa gccagatcca gtccggtaaa accttcgcgg
    22681 tagatcttat aaatcagcgt cgtggtggcc tgaaccggcc cgccggacgt ggcggcgtcg
    22741 atcaccggga aggtgtcgaa gaaggcatac accaggttca ctaccagcag gaagaaactc
    22801 accggggcga taagcggcag cgcaatctta aagaagcggc gaatcggccc tgcaccgtcg
    22861 atggctgcgg cttcgatcaa cgaacgggga atggattgca gcgcggcata gaagaacaga
    22921 aagttgtagc tgatttgctt ccacaccgag gcaaacacca ccagaaacat cgcctgaccg
    22981 ctattttgcg cgtggttcca gtcgtagccg aactcagcga gaaaatgggt gatcagcccg
    23041 cgaccggggt taaacaggaa gatccacaat acggcggcaa cggcgggagc cacggcatac
    23101 ggcagcaaca ttaaggtttg gtagaaacga ctgccgcgca cgatgtactc caccagcgcc
    23161 gcaaagaaca gcgataccag caaaccgctg acggtgacaa aggtgctgaa ttttatcgtc
    23221 gtccagaagg agtcgagata atagctgtca tgaaacagcg tgacgaagtt atccagaccg
    23281 acaaactggc tggagaaacc aaacggatcg acgctttgta gcgagtacca caacgcttcg
    23341 cccgcaggcc agataaaaaa gataacggtg atgatgagct gcggcgcgac cagcagataa
    23401 ggcagccagc gcgagcggaa caccggacgg gatgatgaca ttacggttaa ttcctgaact
    23461 gtgccggatg cgcttcgctt atccggccta cacgcgatgc caggtcagat aagcgaaacg
    23521 catccggcat tacactgatt aagacttcgt cgatttctca aagcggcgca gtaactggtt
    23581 cccacgctca actgcggtat ccagcgcctg ctgtggcgtc ttcttaccgg tccacacgct
    23641 ctcaagctct tcatccacaa tcacgcggat ctgcggcatg ttgcccagac gaagcccttt
    23701 ggtgaacggc aacggcggct tgttcagcat ctgacgcgtc gcggtatccg cacctgggtt
    23761 tttctcgtaa aagccctgct cacgggtcag gtcatacgcc gctttggtaa ttggcagata
    23821 accggttttc tgatgccact cagcagcgtt ttctggcttc gcgaggaagt cgagaaactt
    23881 cgccacgccg gtatacgttt ctttatcttt gccctgcatc acccacaggc tggctccgcc
    23941 gataatggcg ttttgtggcg catctttcgc atcggcgtcg taaggcatca tacctacgcc
    24001 gtagttaaat ttggcgtact cgcgaatgtt ggcaagagaa ccggaagagg cggtggtcat
    24061 cgcgcaatca ccgttataga acttctcggt ggattcatcc ttacgaccga cgtagctgaa
    24121 gtcgcccttc ttgttcatct cctcgagcat ggcgatgtgt ttcacctgct ccggcttgtt
    24181 gaactccagc accgcgtccg tgccgtcaaa gccgttgttt ttgctggcaa acggcagacc
    24241 gttccaggcg ctaaagtttt ccagttggat ccagccctgc cagccgctgg cgtagccgca
    24301 cttaataccg gaggctttca gttttgcgga atagtccgcc agatcctgcc aggttttcgg
    24361 cggctgttcc gggtctaacc ccgctttctt gaaggcgtct ttgttgtaat agagaacgga
    24421 agtcgagctg ttgaacggct gggagagtaa gtggccggtt ttgctgtcgg agtagtaacc
    24481 ggaaaccgtc ggcacaaact gcgactcatc gaactgaatc cctgcctctt taaacacgtc
    24541 atacaccggt ttaatggctt tcgacgccat catggtggcg gtgccaactt cataaacctg
    24601 caaaatagcc ggagcgttgc cggtacgaaa tacggcaatc cccgcgctta aattctgttc
    24661 gtagttgcct ttataggtcg gtacaatttt gtaatccgga ttttcggcgt taaaacgttg
    24721 ggccagagaa tccacctctt tacccagttc cccttccata gaatgccaga acggaatggt
    24781 cgtcactgcc tgtgcattcc ccattaacgc cagtccgagc gccagtgctg aagctgtata
    24841 atgtaacggt ttcatcgttt atctctcttg ttgtaccgaa tgcgcgaatt cacgcgtttt
    24901 atgctcgcgg ggtaacatga catgctcgaa ttacagaaaa ataacttttt tgttacattt
    24961 gtaagatagt aaggtgtcag aaagatgaca aggcggtgac ggcgtgggtg agggaaaatg
    25021 ggagatgggg cacggataag cggaaaaata gaaggtctga atcaaactct acagattact
    25081 catcgtttca tgccggatgc ggcgtaaacg ccttatccgg cctacaagat cgtgcaaatt
    25141 caatatattg caactcaccc agtaggcctg ataagcgcag cgcatcaggc aattttgcat
    25201 ttgtcacctg tctcaaagga gtcttttgac tccttatcaa tcaacgtgtt attacccgcc
    25261 taaatacgca cttctcactg cttcgttcgc cagcagcgcg tcgccggtat cggagagcac
    25321 cacatggccg ttttccagca cgtagccgcg atccgccagc tttagcgcct ggttggcgtt
    25381 ctgctcgacg aggaagatag tcatcccctg ctcgcgcagc tgctcgatgg tgtcgaaaat
    25441 ttgctggatg ataatcggcg caagaccgag cgatggctca tcaagtaaca acaaacgcgg
    25501 attgctcatc agcgcgcgac caatcgccag catctgctgt tcaccgccgg acatggtgcc
    25561 cgcccgctga acacggcgct catgcagacg tggaaacagt tcatacaccc actttatgcg
    25621 ctcctgaaac tggtcacgtt cggcaaagaa gccgcccatc gccaggttct cttccaccgt
    25681 catccgcgag aagacgcgac gcccttccgg gacaatcgct accgcttcgc gcatgatttt
    25741 cgccgtctgc cagtcggtaa tgtctttatc atcaaacaca attcgcccgc tggtggcgcg
    25801 cggatcgccg cataacgtgc cgagcaaggt ggttttcccc gccccgttcg cgccaatgag
    25861 agtgacaatc tcgccctgat tgatatgcag gctcacctca tgcagcgcct ggattttgcc
    25921 gtagtgggcg ctgactttgt caaaggacaa catgactttt tccatcttat gcctcaccta
    25981 aataggcacg gatcacgtcc gggttattac ggatttgctc cggcgtaccg tttgccagtg
    26041 gcgtcccctg attgaccaca taaattcggt ctgaaatccc catcaccagc ttcatatcgt
    26101 gttcaatcaa caaaatagtg gtgttgtgat gattgcgcag ttcggcaatc agctcatcca
    26161 gctctttggt ttcttttggg ttaagacctg ccgccggttc gtcgagcatt aaaatctccg
    26221 gctgcgtcac catgcagcgg gcaatctcca gacggcgttg gtcaccatag gccaggttac
    26281 tcgcctgacg gttggcgtgt tccagcaaac caatgcgctc aagccaggtg gcggcgcggt
    26341 cgagcgcttc gctctgggcg cgacggaaag aaggcgtttt caacaggcca gagaacagcc
    26401 cggttttcag ttgctgatgc tgcgccacca gcaggttttc aattaccgtc atttcacgga
    26461 acagacgcac atgctggaag gtgcgcacca cgcccatgcg agcaatttgc tgccccggta
    26521 aaccttcaag atgctgatcg cgcagtaaaa tggtgccgcc ggtgggtttg tagaaaccgg
    26581 tcaggcagtt aaaaaccgtg gtttttccgg caccgttagg gccgattaac gagacgattt
    26641 cctgcgggta cagttcaaga ttgacgttgt tcaccgccag caggccgccg aagcgcatca
    26701 tcagaccgtt aacagataat aatggctgac tcatgcctgc tctcctttcg ctgcgccgtt
    26761 tttcagcttc agttgcgtgc gcgtcatggg cagcaaaccc tgcggacgcc agatcatcat
    26821 cagcaccatc aaaccaccga gcattaacat gctgtattcg ttgaaatcac gcatcaattc
    26881 gcgcgacacc accagcagaa cagccgccag aatcactgca aattgcgagc ccataccgcc
    26941 gagcaccact atcgccagca caaaggccga ttcagcaaag gtgaaggatt ccgggctgac
    27001 aaagccctga cgcgccgcaa acagcgttcc ggcaaaaccg gcaaatgcgg cgctgatggt
    27061 aaaagcagtc agcttgatac ggcgcgggct taagcccagc gaacgacagg cgatctcgtc
    27121 ttcacgcaac gcttcccacg cgcggcccag cggcatccgc agcaggcggt taatgacaaa
    27181 caggcttaac accaccagca gcaacgccac caggtagagg aagataacgc gatcggacgg
    27241 atcgtatttc aggccaaaga aattactgaa cgtgtcccag ccgccttcac gagcggtacg
    27301 gctgaactcg agtccgaaga gtgtcggttt cgggatctga ctgattccgt tcgggccgcc
    27361 ggtgatttca gtgttattga gcagcaatat gcgcacaatt tcgccgaaac caagggtaac
    27421 gatcgccaga tagtcaccgc gcagacgcag caccggaaaa ccgagcagga aacccgccgc
    27481 cgctgccatc agtccggcaa tcggcaaaca ggtccagaac cccagaccgt aatagtgatt
    27541 gagcagcgca aaagtgtaag cgccgatggc gtaaaaaccg ccgtacccca gcaccagcag
    27601 accagaaaga ccaacaacca cattcagccc gagaccgagg ataatgtaga tcatggtcag
    27661 agtggcgata tccaccgtcc cacgtgacac cataaacggc cacgccacca caagcaccag
    27721 cagcgccacg aggaacagct tttgcttcac cgtggaccca tcaatcgcgg gcagaataaa
    27781 cttcggcccg gaaacgcttt tcaacccttt ctggaaagcc ggtcgcaaaa gctggaagaa
    27841 aaagaccacc gccgtgccga taaacatcca ctgccaacgg atgtctgaag ccgtgtcgac
    27901 caccagtttg gtgccatcca gctccagttg tacgcccata aagacgcccg ccaggacaaa
    27961 gaacatcgcg gcagagagca gtgccattgc aatatgcatc ggtttcatac tttctctacc
    28021 tccgggcgac ccagaatacc ggtcggcatc accagcagca ccagaatcag cagggcgaat
    28081 gagaccacat ctttatattc cgtactcaga taggcagaag agagcgcctc cgcaatcccc
    28141 aggatcagcc cgccaatcat cgcccctgga atgctgccaa tcccaccgag caccgcagcg
    28201 gtaaaggctt tcatcccggc cataaagccg atataggggt taatgacgca gtagaactga
    28261 ccgagcagta cgccagccac tgccgccatc gccgcgccaa taacaaaggt caaagcaatc
    28321 acccggtcgg tgttgatgcc aagcaggctc gccattttca gatcttccgc gcaggcacga
    28381 caggcgcgcc ccatacggga atagcgaatg aaaatcgtca gcgccagcat ggcgaggaag
    28441 gtgacaatcc agatcaccgc ctgcatggtg gtaatagagg cagagaagtt ttcgctatgg
    28501 cccaccaccc actgaccgtt aaacaggctc ggcagcgcca cgtcgcgcga accttcggtc
    28561 aggctgacgt agttttgcag gaagatggac ataccgattg cggaaataag cgcaatcagg
    28621 cgcttagagt tacgcaccgg gcggtaagct acccgttcga tactccagcc gtaggcgctg
    28681 gcgatgacga ttgcgccgac gaatcccgcc gccaccagca gccagccggt atcaatgccc
    28741 atcatcatca gcgcggcgat gatcataaat gagacgtagc tgccaatcat ataaacctcg
    28801 ccgtgggcga agttgatcat gccgataatg ccgtaaacca tggtgtagcc gatggctatc
    28861 agcgcgtagg tactgcccag cgtgacgccg ttaaacatct gctgcaagaa atacaaaaac
    28921 tgctcagaca taaggtaacc tttctaaacc cgcccgcatt ttacgggcgg tgggatgatc
    28981 acttggctgc cgtggatgaa ccgtcggcgt gccactggaa gacaccaaaa tcaaatccct
    29041 taagatcgcc tttttcatcc cagttcagcg gcccaatcac ggtgtttgca ccgttagctt
    29101 ttaaatcttt caccagcgcc agcggctcat cgctgccggt acgctcaagg gcagttgcca
    29161 gagattgcac tgccgcgtaa gtgatccaga cataaggccc ggacggatct ttcttgtctg
    29221 ctttcagcgc atcaacgatg ccctggtttg ccggatcctg gtcatagcgt tttggcatag
    29281 tgaccaacat gccttcggcg gcatcaccgg caatgttcga cagcgatgcg ttaccgacac
    29341 cttccggccc cataaactgg gttttcaggc caacggaacg ggcttggcgc agcatctgcc
    29401 ccatttccgg gtagtaaccg ccgtagtaaa cgaagtcgat attttctttt ttcaggcggg
    29461 cgatcagcgc ggagaaatct ttctccccgg cggtaatacc gtcgaagaag acgacgttgg
    29521 cgttagccgc tttcagcccg tcctgcaccg aacgcgccag cccttcgcca tactgttgtt
    29581 tgtcgtgaat gatggcgatg cgctggggct tcaccgtctc aagaatgtat tttgccgccg
    29641 taggcccctg ggaagagtcc agcccggcag tacgcataat gtgttgataa ccgcgttggg
    29701 tcagctccgg gttggtcgct cccggcgaga tcatcagaat accttcgtct tcgtagatat
    29761 ccgacgctgg ctgggtagag gaagaacaca gatgaccaat aacgtattta atgccgtcgt
    29821 taacgatttt gttggcgacc gcaacggctt gtttcgggtc gcaggcgtcg tcatattcca
    29881 cgccaaccag cttgtcgccc ttaattcccc ctttgacatt aatgtcttta atcgcctgac
    29941 gcgcgccgtt aaattccata tcgccccact gggcaatcgg gccggacatc gcgccgacaa
    30001 cggcgacttt aatatcgtca gccatagcgg tgtgtgaaat tgccagtgca atcatccctg
    30061 cgatgatagt tttcgcattc cgtttcatag tcaaaaatcc ccattcgtga tgttgtgttg
    30121 ctttgttttt atgtgttaac aaatcagact gttctttttt tatactgcac tgtttttgcc
    30181 tgtctgatta aggggttagc gcagtatttt gtgatgatag cgattaaaat ccctattttt
    30241 cagtcgatta agaacagata atattctgaa tttattgata gataaacaga aaaaagtgcc
    30301 tttgtcagca taaaataacg gcacaaaggg cggaataatt cactatcatt caggggatta
    30361 tgctggacat ttttcattct ctaatgtttt aactttgtaa ttattgctgt taaaaaatta
    30421 atcacctgcc aaaagaaata aaaaagagaa agcctccgat taaattattt cgctacactg
    30481 gttccacttt tgtgatttac acgggttacc catgaagctg accatcattc gattagaaaa
    30541 ctttagcgac caggaccgga ttgacctgca aaagatctgg ccggagtatt ccccttcctc
    30601 tttacaggtt gacgataacc accgtatcta cgccgcgcgt tttaacgagc gtctgcttgc
    30661 ggccgtgcgg gtaaccttaa gcggcaccga gggagcactg gattctctac gcgtgcggga
    30721 agtcacccgc cgtcgcggtg tggggcaata tctgctggaa gaggttttgc gtaacaatcc
    30781 tggcgtttca tgctggtgga tggcggatgc aggcgtggaa gatcgcggtg tgatgacggc
    30841 gtttatgcag gcactgggat ttacggcaca gcagggcggc tgggagaagc gttaatcgtc
    30901 aagttgattt caaaagtgat gaccaacgag aaattgcctg atgcgctacg cttatcaggc
    30961 ctataatgtg tgctgcaatt tactgatttc tttggttctt gtaggccgga taaggcgttt
    31021 acgccgcatc cggcatgaag caacgtattc gatattagca atttggcggc aacccaaagt
    31081 tgccgattaa caattacttc gcatcggtcg ccgtgccgtt ggcgtgccag tcaaatacgc
    31141 cgaactcaaa gccctttaga tcgcctttct catcccaggt cagtggcccc atcacggtat
    31201 ccacggagtt cgcttttagg tatttggcga tttcagccgg atcgtcagac tgattcaggc
    31261 ccgcctgcaa agattgcagc gcggcgtagg tggtccagac gaatgcgccg cttgggtcct
    31321 gttttttcgc tttgatcgca tcaacaatgg gtttgttcgc cggaacctga tcgtagttct
    31381 tcggcttggt caccagcagc ccttccgctg attcgcccgc aatgttagac agcgaaacgt
    31441 tagccacacc ttccggcccc ataaactgag ttttcagccc tgccgcgcgt gcctgacgca
    31501 ggatttgccc catttccggg tgataaccgc cgtagtaaac gaagtcgata ttctcttttt
    31561 tcagacgcgc caccagcgtt gagaaatctt tttccccggc ggtgatacca tcaaagaaca
    31621 ccacgttagc attgcctttc ttcaggccgt cctgcaccgc tcgtgccaga ccttcgccgt
    31681 attgctgttt gtcgtgaacg atagcaatac gctgcggttt cactttctca agaatatatt
    31741 tcgccgccgt cggcccctgg tcggagtcca ggccggtggt gcgcaggatc agctgatagc
    31801 cacgggcggt cagctccggc gcggttgccg ctggggtgat cattaaaatg ccttcgtctt
    31861 cgtagatgtc agacgcaggc tgcgttgatg aagaacagag gtgaccaatc acatatttaa
    31921 tgccgtcgtt aacgactttg ttcgccaccg caaccgcctg tttcgggtca caggcatcgt
    31981 catattttac gatttgcagt ttgttgcctt taatgccgcc tttagcgttg atatccgcaa
    32041 ccgcctgctc tgcgccggta aactcctggt caccgtactg cgcaaccgga ccggacattg
    32101 cacccacgac cgcgacttta atatcttctg ccagagccat attgctgaat gccagcgcga
    32161 tacatcctgc cagtaacgct ttacccttta tgttcatcct gagaatcccc attcttctgg
    32221 ttattacgtg tgttgtgatg ttgttgttca gcactttatt tcgttttatg catgactacc
    32281 cgtgctttag cagcatactc tgctaaaaca tacccgattt ttatgatatt ggaatagcta
    32341 ttttgacagt ttattaacaa tctgcgtggg gattggcgtt ttgccggagg gggaattgat
    32401 tattgtagag gcccaaaaaa caaaaacccc ggactctcat ccagggttct ctgcttaata
    32461 gcggaaatta cgcttcaata gcggcgcgaa gttttttcat cgcgttcttt tccagttggc
    32521 gcacacgttc agcggaaaca ccgtaacggt cagccagttc ctgcaacgtg gacttgttgt
    32581 cttcgtccag ccagcgcgca cggatgatgt cctggctgcg ttcgtccaga ccatgcatcg
    32641 cgtcggtcag acggtttgcc gcctgctctt cccagttatc atcttcgatg ccgtcggcaa
    32701 agttagatga tttatcctgc agatagagca ccggtgccat cggctggctg tcggaatcgt
    32761 cgtcggaaga caggtcaaag gtcatgtcct gtgccgccat acgtgattcc atctcacgta
    32821 cgtctttgct ggttacgccc agttcacggg ccaccatttc gacttcatcc tggttaaacc
    32881 agcccagacg ctgcttggtt ttacgcaggt tgaagaacag tttgcgctgc gctttggtgg
    32941 tcgcaacttt gacgatacgc cagttacgca gaacgtattc gtggatctct gctttgatcc
    33001 agtggacggc gaaggagacc aggcgcacac ccacttccgg gttaaaacgg cgcactgctt
    33061 tcatcaggcc gatgttacct tcctgaatca aatccgcctg tggcaggcca tagcccgcat
    33121 aattacgagc aatatgaaca acaaaccgca ggtgagacag gatcagcgtt ttagctgctt
    33181 ccagatcgcc atggtaatgc agcttttcag ccagcgcccg ctcctcgtca gccgacaaca
    33241 tcggccacgc gttagctgcc cggatgtagg aatccaggtt gccaactggg gctaaagcta
    33301 aactttgcat tttgtcagtc attcaaatcc tctcaatcga tatcttctgg cgcttcagtg
    33361 gtagcaacaa ctgtgccaaa gcttaagagc aacgaggtta tcattcactg ttttatcaga
    33421 ccgtgatttt atccacaagt tcaatgcaag cttgtgaata aattacgcac aaaatgtgac
    33481 atagagatga aataccggga agagacaacg gggtctcttt ccctgctacg gaacccattg
    33541 cagggaaaga gtataacacg cttttattat tcaggcgtaa agtggcgtaa atgttgtacc
    33601 gtggcaagcc acgctgccac ccagccaatc atcgagcata ccagcagcaa tagcaggcat
    33661 tcatcgaacg ataaaccatt gatatcaaac ttcgttccga aaacctgtgt cacttccgcc
    33721 accgctgacg acaaacgcag taccagaatt tctgacaaaa ttaatgacaa caatgcgcca
    33781 gaaaatccca gcagcgcacc gccgtacagg aacgggcgaa ggatgaaacc atccgtcgca
    33841 ccaatcagtt tctgcacgtt gatggagtca cggcgagcaa agatgctcag acgtacgctg
    33901 ttgccgatga cgaggaacac tgccgccacc atcaacacgc cgatcatcgc cgaaacgcgc
    33961 ccaaccagcc cggtcaacgc cgccagacgg gcaaaccagc tgtcatccat ccgcacttcg
    34021 tcaataccgt taatctgcgt gatacgatca cgcagcgtat tgagtgattc cgtcccctga
    34081 aaatcgagtt tcgggatcac caccgccact gccggaagcg ggttttcttc cagcatatcc
    34141 agcgcaccac caaaaccaga ccagttacgg aactcgccca gtgcgtcttc acgagaaaga
    34201 tagttcactt tctccacgcc ttgctcggcc tgcaactgtg ccaccacgcc cgcagcggcg
    34261 tcatcgtcca gcgttttttg caagtaaaca gtgatttgcg gtgacggata atactgcgtc
    34321 gccgcctgat taacgttttt gtacaccata taacagacgc tgggcagcgt cagggagatg
    34381 gcgataacca tcaccgttaa aaacgtggcg aacggtttgc ttttcagatc ctgcaatgcg
    34441 ccgtggaagg catagcgcac ctgttcgttg aaaacgttgg ttttgcgatt taccggtttt
    34501 ggcgaggatt tcgcgcgttt cggtgcgtta cgaccgccgt cgcctgagcc gccgaccgat
    34561 ttacggaagc gatccagacg cccaccaaac tgccgaatat gattgattgc atcgcgctta
    34621 ttcatggccc acgcctccat gcaagtgacc atcgctcagg gtgagcatgc gataggaacg
    34681 ccgtgaaatc agattaatgt cgtgcgttgc catcaatacg gttaccccaa cgcggttaaa
    34741 ctcttcaaac agacgtagaa tcccttccga caacgcgtcg tccaggttac cggtcggttc
    34801 gtccgccagc agtaccgcgg gcttattcac caccgcgcgg gcaatgccaa cacgctgttg
    34861 ttcaccgccg gaaagctgaa tagggaagtt cttcgctttg tccagtagcc cgactttatc
    34921 cagtgccgcc gacacccggc gacgaatatc gtcaccgctg gcaccagcga taatcagcgg
    34981 gatcgccacg ttatcgtaga cagtacggtc catcagtaga tggtgatcct ggaaaatcat
    35041 gccaatctgg cggcgcagaa acggaacttc acggtttttc agacgcgtga tgtcatggcc
    35101 gctaaaccag attttcccgg cgctgggccg ctcaatccca cagatcagct tcaggagggt
    35161 acttttccct gcgccggaat gaccggtcag aaacgccatc tcacccggct gcatatggaa
    35221 cgtaacgccc tgcagcgcct gtctcccacc gagataagcc ttgctgacat gttcaaagcg
    35281 aatcattgtt aatcctctcg ggcaaaaagt gcctctataa agtcgtccgc cttaaacgga
    35341 cgcaaatcct caatacgttc gccgacacca atgtagcgga tagggatacc aaactggtca
    35401 gccaccgaga aaattacccc gcctttcgcc gtgccgtcca gtttcgttag cgtgatgccg
    35461 gttaagccaa cagcttcgtg gaacagtttg gcctggctta ccgcgttctg cccggtgctg
    35521 gcatcaatag tcagcataac ttcatgcggc gcttcaacgt cgagtttctt catcacgcgg
    35581 acgattttct tcaactcttc catcagatgc gatttgttct gcagacgtcc ggcggtatcg
    35641 gcaatcagga cgtcgatatt acgcgcttta gctgcctgaa tggcgtcgaa gataacagag
    35701 gcggaatccg caccggtatg ctgggcaatc accggaatat tgttgcgctg accccagacc
    35761 tgaagctgtt caaccgcagc tgcacggaaa gtatcgcccg ccgccagcat caccgattta
    35821 ccctgctgct caaactgacg cgccagctta ccaatcgtcg tggttttacc cacaccgttg
    35881 acgcccacca tcaggatcac gaacggcgtt ttatcttcaa cattcagcgg ctcatcgact
    35941 ttcgccagaa tctcgcccat ctcttctttc agcaggccat agagcgcctc ggcatcacga
    36001 agctgcttgc gggatgcgcc ttccgtcaga ttggtgataa ttttacgtgt ggtttccacg
    36061 cccacatcgg cgatcaacag ctgttcttcc agctcttcaa acagatcatc gtcgattttt
    36121 ttaccgcgga acaggctgat aaatccggaa ccgagatttt ctttggtttt taacaggctg
    36181 cgtttcaggc gcgcgaagaa gccttctttg gtcggttttt cttgctcctg ggcaatttct
    36241 gcaaccggct gctcttcttc tgccggagga actaccatca ccgcctcttc tgccgcttcg
    36301 gcagccagcg cctgtgcttc cagctcttcg tcggtgattt cttctttagc cgcttcttct
    36361 tctgccgctt cgacaatctc tacggtttcc gcttcagcct gccactcttc tggcgaaacc
    36421 tcttcggcgt tgacgtcttc cggcaacggc agctcttcac gttcgatagc cactggctcc
    36481 ggcgtttctt ctacgaccgg ttccggctgt gcaacaactt ccgcttcagg ctgcgctttt
    36541 tcactttcaa caacctgttc agtgacttcc acaacgtcgg cagcaaaagt ttccgcctcg
    36601 gcttcagtat gcgcctgcgg ctgctcttca acggcttgtt cagaagcctt cgcaggctct
    36661 tgcgcctgaa cgatttcttc tacaaccggt tgttcattct gaacttctgt ctctttttcc
    36721 ggggtctgct ctttttgacc aaagcccagc caggaaaaaa agccacgtct tttttctttc
    36781 gccatttgcg actacactcc tcgctgttgc ttcatggcac agcgttaacg ctatgtacat
    36841 agcagctaaa aaaatgatga aatagtctat cacttaactt aattcacatc accgcctgca
    36901 agtatgtgtt atctggcgga ttgagcaatt tatcatgaaa aaaccgaatc attccggcag
    36961 cggccaaatc cgcattattg gcgggcagtg gcgaggccgt aaactcccgg ttcctgatag
    37021 cccaggtctg cgccccacca ccgaccgcgt acgcgaaacg ttgtttaact ggctggctcc
    37081 ggtcattgtt gacgcccaat gtctggattg cttcgccggg agcggcgcgc tggggctgga
    37141 agcgttatcg cgctacgctg cgggggcaac gttgattgag atggatcgcg tggtttctca
    37201 gcagttaatt aagaatctgg cgacactaaa agcaggcaat gcacgcgtgg tgaacagcaa
    37261 cgcaatgtca ttcctggcgc aaaaaggcac accgcataat atcgtgtttg tcgatccacc
    37321 gttccgccgt ggcttgttag aagagacgat aaatttactg gaagataacg gctggctggc
    37381 tgacgaagcc ctgatttatg tcgaaagcga agtcgaaaac ggtctgccca ctgttccggc
    37441 aaactggtca ttacatcggg aaaaagtggc gggtcaggtg gcttatcggc tgtatcaaca
    37501 cgaagcacaa ggagaaagtg atgctgatta atattggtcg tttgttaatg ctctgcgttt
    37561 ggggattttt aatcctcaat ctggtgcatc ccttcccgcg cccgctaaat attttcgtta
    37621 acgtggcgct gatttttacc gtgctgatgc atggtatgca gctggcgcta ttgaaatcca
    37681 ctttaccgaa agatggcccg cagatgacca ccgccgaaaa ggtgcggatt ttccttttcg
    37741 gcgtgtttga actgctggtc tggcagaaga aatttaaagt taaaaaataa ccttattgtt
    37801 cgccaacaaa gctaacaaag cgcgttcctt tatagctcag cgtaccttta tcgcccactg
    37861 tcagggcgtg gtactgctgg gcgtcgaggc gaaacgtctg ctccattcct ccgctttgcg
    37921 gtttgaagct tgcctcatag cgcatactgg tgcctgccgg agtcacttcc tgctggcgcg
    37981 aacggcgatc gttaatcggt ttttcccgct tgttgctcac caccaccagc ttttgctgga
    38041 gcggagccat atcattatca gctttttccc gtcgctgttg cataaaacga aacgatgcgg
    38101 cgacgacaat taagccaatg ataacaataa agaaaagagg tggtttactc atctttatcc
    38161 ctcatcggaa aatgcggaat taagcatacc ctgccagtta tggtgttgtc atccgtccac
    38221 cctcgccact aaactggaag caagaccgta ggcattctgc ttacgaaaaa ataacgaatt
    38281 caaggaacta agatgctttg gtcgtttatc gctgtctgtc tttccgcatg gctatctgtg
    38341 gatgcatcgt atcgtgggcc aacctggcaa cgctgggtgt ttaaaccgtt aacccttctt
    38401 ctcctgctgt tactggcctg gcaagcaccg atgttcgacg ccattagcta tctggtgctg
    38461 gcgggactgt gcgcctcact gctgggcgat gcgctaaccc tgttgccacg tcaacgtctg
    38521 atgtacgcca tcggcgcgtt tttcctctcg cacctgctgt acaccatcta tttcgccagt
    38581 cagatgacgc tctctttctt ctggcctctg ccactggtgt tgctggttct gggtgcgctg
    38641 ctactggcga ttatctggac gcggctggaa gagtaccgtt ggcctatctg cacgtttatc
    38701 ggcatgacgc tggtgatggt gtggctggca ggtgaactgt ggttcttccg tccgaccgct
    38761 ccggcgctct ctgcgtttgt tggcgcttcg ttgctgttta tcagtaactt tgtctggctg
    38821 gggagccact atcgccgacg cttccgtgcg gataacgcga ttgctgcggc ctgctacttt
    38881 gccggtcact tcctcatcgt ccgctcactg tatctctgat aaaacttgac tctggagtcg
    38941 actccagagt gtatccttcg gttaatgaga aaaaacttaa ccggaggatg ccatgtcgac
    39001 acctgacaat cacggcaaga aagcccctca atttgctgcg ttcaaaccgc taaccacggt
    39061 acagaacgcc aacgactgtt gctgcgacgg cgcatgctcc agctcgccaa cgctctctga
    39121 aaacgtctcc ggcacccgct atagctggaa agtcagcggc atggactgcg ccgcctgtgc
    39181 gcgcaaggta gaaaatgccg tgcgccagct tgcaggcgtg aatcaggtgc aggtgttgtt
    39241 cgccaccgaa aaactggtgg ttgatgccga caatgacatc cgtgcccagg ttgaatctgc
    39301 ggtgcaaaaa gcgggctatt ccctgcgcga tgagcaggcc gccgacgagc cacaagcatc
    39361 gcgcctgaaa gagaatctgc cgctgattac gcttatcgtg atgatggcaa tcagctgggg
    39421 tctggagcag ttcaatcatc cgttcgggca actggcgttt atcgcgacca cgctagttgg
    39481 gctgtacccg attgctcgtc aggcattacg gctgatcaaa tccggcagct acttcgccat
    39541 tgaaacctta atgagcgtag ccgctattgg tgcactgttt attggcgcaa cggctgaagc
    39601 tgcgatggtg ttgctgctgt ttttgattgg tgaacgactg gaaggctggg ccgccagccg
    39661 cgcgcgtcag ggcgttagcg cgttaatggc gctgaaacca gaaaccgcca cgcgcctgcg
    39721 taacggtgag cgggaagagg tggcgattaa cagcctgcgg cctggcgatg tgattgaagt
    39781 cgccgcaggt gggcgtttgc ctgccgacgg taaactgctc tcaccgtttg ccagttttga
    39841 tgaaagcgcc ctaaccggag aatccattcc ggtggagcgc gcgacgggcg ataaagttcc
    39901 tgcaggagcc accagcgtag accgtctggt aacgctggaa gtgctgtcag aaccgggtgc
    39961 cagcgccatt gaccggattc tgaaactgat cgaagaagcc gaagagcgcc gcgcccccat
    40021 tgagcggttt atcgaccgtt tcagccgtat ttatacgcca gcgattatgg ccgtcgccct
    40081 gctggtaacg ctagtgccgc cgctgctgtt tgccgccagc tggcaggagt ggatttataa
    40141 aggtctgacg ctgctgctga ttggttgccc gtgtgcgtta gttatctcaa cgcctgcggc
    40201 gataacctcc gggctggcag cagcagcgcg tcgtggggcg ttaattaaag gcggtgcggc
    40261 gctggaacaa ctaggtcgtg ttacccaagt ggcgtttgat aaaaccggta cgctgaccgt
    40321 cggtaaaccg cgcgttaccg cgattcatcc ggcaacgggt attagtgaat ctgaactgct
    40381 gacactggcg gcggcggtcg agcaaggcgc gacgcatcca ctggcgcaag ccatcgtacg
    40441 cgaagcacag gttgctgaac tcgccattcc caccgccgaa tcacagcggg cactggtcgg
    40501 gtctggcatt gaagcgcagg ttaatggtga gcgcgtgttg atatgcgctg ccgggaaaca
    40561 tcccgctgat gcatttgctg gtttgattaa tgaactggaa agcgccgggc aaacggttgt
    40621 gctggtagta cgtaatgatg acgtgctggg tatcattgca ttgcaggata ccctgcgcgc
    40681 cgacgctaca actgccatca gtgaactgaa cgcgctgggg gttaaaggag tgatcctcac
    40741 cggcgataat ccacgcgcag cggcggcaat tgccggggag ctggggctgg agtttaaagc
    40801 gggcctattg ccggaagata aagtcaaagc ggtgaccaag ctgaatcaac atgcgcccct
    40861 ggcgatggtc ggtgatggta ttaacgacgc gccagcgatg aaagctgccg ccatcgggat
    40921 tgctatgggt agcggcacag acgtggcgct ggaaaccgcc gacgcagcat taacccataa
    40981 ccacctgcgc ggcctggtgc aaatgattga actggcacgc gccactcacg ccaatatccg
    41041 ccagaacatc accattgcgc tgggactgaa agggatcttc ctcgtcacca cgcttttagg
    41101 gatgaccggg ttgtggctgg cagtgctggc agatacgggt gcgacggtgc tggtgacggc
    41161 gaatgcgtta aggttgttgc gcaggagata aggcaaaccg atcgcaacat tgagcgcgat
    41221 cggtcccctc gcccctctgg ggagagggtt agggggtgag gaaaaggcgg catcgaagcc
    41281 aatcagcccc tatcaaccgc ctttacgaat caaataacga taaggcagtc catccgtctc
    41341 tttcgcaacc agttcgtgtt ccataaaggt acaaaaccca ggaatatcgc gggtagtggc
    41401 cggatcgtcg gcgataatca gcaacgtttc gccaggctgc atattgcgca cggttttgcg
    41461 caccatcatc accggttccg ggcagcgcag gccaagcgcg tcgagtgtgt ggtcagggct
    41521 ggaaaagaga tcggtcattt tcttctcatc acttaaaaaa acggcgctag tttacgccct
    41581 gtgagtccgt aagcaaacca ggttaacgat tgcgtgaaaa ttaaccattg cattgtcaac
    41641 gtaaagcagt atcatgcggc ggctcgaaaa aagggtaagc acgttattat gttaaggtaa
    41701 cagacgtgtc gtacgtattg ggttccctca ccccaatggt taatcaaaaa ggtacaatat
    41761 gaacgttttc tcgcaaactc aacgctataa ggcgttgttc tggttatcgt tatttcatct
    41821 tctggtgatc acctccagta actatctggt tcagcttccc gtctccattt ttggtttcca
    41881 taccacctgg ggcgcgttta gctttccgtt tatttttctt gctaccgacc tgaccgtgcg
    41941 tatttttggc gcaccgctgg cccgacgcat tatcttcgcg gtaatgatcc ctgcgttatt
    42001 aatctcctac gtcatctcgt cgctattcta tatgggttcc tggcagggat tcggcgcact
    42061 cgcccacttc aacctgtttg tcgcccgtat cgccaccgcc agtttcatgg cctacgcgct
    42121 ggggcaaatc ctcgacgtgc acgtttttaa ccgcctgcgt cagagtcgcc gctggtggct
    42181 ggcaccgaca gcgtccacac tgttcggtaa cgtcagcgac acgctggcct ttttcttcat
    42241 tgccttctgg cgtagcccgg atgcctttat ggctgaacac tggatggaaa tcgcgctggt
    42301 cgattactgt ttcaaagtgt taatcagtat cgttttcttc ctgccaatgt atggcgtatt
    42361 actcaatatg ctgttgaaaa gactggcaga taaatccgaa atcaacgctt tgcaggcgag
    42421 ttaaaggttc gttatcagag ttgtgataag atggatgaat gagccgttat ggccgtttat
    42481 cgaaaggaag aagtcaatgc gcaatctggt taaatatgtc ggaattggcc tgctggttat
    42541 ggggcttgcg gcctgtgatg ataaagacac taacgctacg gcgcagggtt cggttgcgga
    42601 aagtaacgct accgggaatc ccgtcaacct gcttgatggc aagttaagtt tctcgctgcc
    42661 agcggatatg accgaccaga gcggtaagct gggaacgcag gccaataaca tgcatgtctg
    42721 gtccgacgcc accgggcaga aagcagtcat cgtcatcatg ggcgatgatc cgaaagaaga
    42781 tctggcggtg ttggcgaagc gtctggaaga tcagcaacgt agccgcgatc cgcagctgca
    42841 agtggtaacc aataaagcca ttgagctgaa aggtcacaaa atgcagcagt tagacagtat
    42901 tatctccgcg aaaggccaga cggcgtactc ttccgttatt ctgggtaacg tgggtaatca
    42961 actgctgacc atgcaaatta cgctgccagc tgacgatcag caaaaagcgc aaaccaccgc
    43021 agaaaacatc attaatacgc tggttattca gtaagtttta agatgatgag gcggcctcag
    43081 ggacgtgttc cggaggccgt ttttttaatc gccacgtcag taataacgcg attgcgacca
    43141 gtcccgccgc cgccagataa atcaccggta cgcccgccca gctcatcacc agcccagcca
    43201 gtggtccagt cacgccaagc gataaatcca taaatacggt gtaagttgcc agcgccgccc
    43261 cctgattttg ctgcggaacc gcttttaccg ccactacacc caatgccggg aacaccagcg
    43321 aaaacccggc ccccgccagt aagacgccga ttttcgccat ccacggcata gtcgccacgc
    43381 caaccagtag caggccgatt atctcaacgc taaagcaaat catcgctacg tttaagccac
    43441 cgatacggtt aatgccgtta gggaataaca aacgcgtacc gacaaacgca cagctaaaca
    43501 gcgtcagcgc gaaagccgca ccgtcccaac ctttagcgtc ataaaacagc gtgataaagg
    43561 tggcgatgac gccaaatccg gcggaagcca gtgccagcgc cataccgtac agccagacgc
    43621 gcccaagcac cgcgcgaaac ggcagcggtt tgcctttact ggcttttacc gtcggacgcg
    43681 ggatcgccaa caaaatggcc accagcgcca cgcccataat gattaacgct aacgcctgca
    43741 agccgcccca gtgataaaac acgacgccta acggcgcacc catcgccatc gccccgtaag
    43801 tgacaatgcc gttccacgaa atcacccgcc cgatatgcag cgagccaacc actccaacgc
    43861 cccatagggt cgatcccgtt ccggcaaaac tttgcccaat cccaaggatg acgcgcccca
    43921 ggcaaagtaa taacaggctg atgacaggca gactggcggt taatcctgcc gtcagatacc
    43981 ccagaccgct caaaaagcag ccgcataaac cgaagacgac aatctttttg ggtcccagca
    44041 aatcggcgta acgtccggca tgagggcggc tcagcaaggt ggcgaaatat tgcaggctga
    44101 taaccaatcc tgcccagaag gcgctaaagc ccatcacatc atggacatag cccggtaata
    44161 cagcgagcgg caacccgatg gtgaggtagc tggcgaagtt aaacatgaca atggagacaa
    44221 tgcgcaaatt caggcgcaat ccgtttagcg cgggttcggc tacgggttcg ggcatgagga
    44281 tcaccacatt tttacaacag tgtttcattt ttaccatgtg ctgacgtgaa aatcagcagt
    44341 aagaatcaga atattgctgg cgtgactccc gcgctacact taacacaaaa agtcacaagg
    44401 aagccccaat ggaaacccct caacccgata aaacgggcat gcacattctg ctcaagttgg
    44461 cctcgctggt agtgatcctc gcgggcattc acgcagcggc agatatcatt gtgcagctgt
    44521 tactggcgct gttttttgcc atcgtcctca acccgctcgt tacctggttt attcgtcggg
    44581 gagtacaacg ccccgttgcc attacgattg tagtggtggt gatgctgatc gcactaaccg
    44641 cgctggtcgg cgtactggcg gcatcgttta acgaatttat ctctatgctg ccgaagttta
    44701 ataaggagct gacgcgcaaa ctttttaaat tgcaggagat gttgcctttt cttaatttgc
    44761 atatgtcgcc ggagcgaatg ctgcagcgga tggactcgga aaaagtggtt accttcacca
    44821 cagcgctaat gaccgggctt tccggggcaa tggcgagcgt gcttttgctg gtgatgaccg
    44881 tagtttttat gctgtttgaa gtgcgccacg tcccttacaa aatgcgtttt gcgctgaata
    44941 acccacagat tcacatcgca ggattacacc gcgcacttaa aggcgtttcg cattatctgg
    45001 cgttaaagac gctactcagt ttatggacag gtgtcatcgt ctggctgggg ctggcgctaa
    45061 tgggcgtaca gtttgcgctg atgtgggcag tactggcgtt tttgctcaac tacgtgccca
    45121 atatcggcgc ggtaatttcc gccgtaccgc caatgattca ggtgctgctg tttaatggtg
    45181 tttacgaatg tattctggtc ggcgcattgt ttttagtggt ccatatggtc atcggcaata
    45241 ttttagaacc acggatgatg ggccatcgcc tggggatgtc caccatggtg gtatttcttt
    45301 cattgttaat ctggggatgg ctgctcggcc cggtagggat gctactttcg gtgccattaa
    45361 ccagcgtgtg taaaatctgg atggaaacga cgaaaggtgg cagcaaactg gcgattttac
    45421 tggggccggg cagaccgaaa agtcggttac cgggatgagg cgacaagtga tacgatacgc
    45481 actttcattt tccattaaac gttggccctg atatgtatcg gatagttctg gggaaagttt
    45541 cgaccttaag cgcagctcca ctgccaccgg gtttacgcga gcaagcaccg caaggtccac
    45601 gacgcgaacg ctggctggcg gggcgtacat tgctttcgca cacgctttcc ccgctaccgg
    45661 agatcatcta tggcgaacaa ggcaaacctg cattcgcgcc ggaaacgccg ctatggttca
    45721 acttaagcca tagcggtgac gatatcgctc tgctgttgag cgatgaaggc gaagtcggct
    45781 gcgatatcga agtgattcgc ccgcgcgtca actggcgctg gctggcgaac gccgtgttca
    45841 gcctcgggga acacgctgaa atggacgccg tgcatcctga tcagcaactg gaaatgttct
    45901 ggcgcatctg gacgcgcaaa gaagccatcg ttaaacagcg tggcggcagc gcctggcaaa
    45961 tcgtcagcgt agatagcacc tatcactcct cgctgtcagt cagccattgt cagctagaaa
    46021 atttaagcct tgcgatctgc acccccaccc cctttacgct caccgccgac agtgtgcaat
    46081 ggatcgattc agttaactga tccgcccacc cgactgccca tctattgatc cagaacaggt
    46141 aatcagtatg acgaatactt aaaatcgtca tacttatttc cgccatctat tttaatccat
    46201 tggggttacc atgctctcca cactccgccg cactctattt gcgctgctgg cttgtgcgtc
    46261 ttttatcgtc catgccgctg caccagatga aatcaccacc gcctggccgg tgaatgtcgg
    46321 gccactaaac ccgcaccttt acacgcctaa ccagatgttc gcccagagca tggtttatga
    46381 accattggtg aaatatcagg cagacggttc ggtgatcccg tggctggcaa aaagctggac
    46441 tcattcagaa gatggtaaaa cctggacctt caccctgcgt gatgacgtga aattctccaa
    46501 cggtgaaccg ttcgatgccg aggcggcggc agaaaacttc cgcgcagtgc tcgataaccg
    46561 tcaacgtcac gcctggctgg agctggcaaa ccagattgtt gatgttaaag cactcaataa
    46621 aacagagctg caaattaccc tgaaaagcgc ctactatcct ttcctgcaag aactggccct
    46681 gccccgtcct ttccgtttta tcgctccctc gcagtttaaa aaccatgaaa ccatgaacgg
    46741 aattaaagcg ccgattggca ccggaccgtg gattttgcag gaatcgaaac tgaatcagta
    46801 cgatgtcttc gtccgtaacg aaaactactg gggcgaaaag ccagcgatta aaaagatcac
    46861 ctttaacgtc atcccggacc cgactacccg cgcggtggcg tttgaaactg gcgatatcga
    46921 cctgctgtac ggaaacgaag ggttattacc gctcgatacc ttcgcccgct ttagccagaa
    46981 tccggcttac cacacccaac tgtcacagcc gatcgaaacc gtgatgctgg cgctcaatac
    47041 cgccaaagcc cccaccaacg agctggcagt acgtgaagct cttaattacg cggtaaacaa
    47101 aaaatcgctg attgataacg cgttatatgg cacccagcag gtcgccgaca ccctgtttgc
    47161 cccttctgtg ccttacgcta atctcggcct gaaaccgcgc cagtacgatc cgcagaaagc
    47221 gaaagagttg ctggaaaaag ccggttggac gctgcctgcg ggcaaagaca tccgcgagaa
    47281 aaatggtcag ccgctgcgca ttgaactgtc gttcatcggc accgatgcgt taagcaaatc
    47341 gatggcggaa atcattcagg ctgatatgcg ccagattggc gcagatgtct cgctgattgg
    47401 cgaagaagag agcagtatct atgctcgtca gcgcgacggt cgttttggca tgattttcca
    47461 ccgcacctgg ggcgcgccat acgatccaca cgccttcctg agttcaatgc gcgtaccgtc
    47521 gcacgctgac ttccaggcac agcaaggatt agccgacaag ccgctgattg ataaagagat
    47581 cggcgaagtg ctggcgaccc atgacgaaac gcaacgtcag gcgctgtatc gcgacattct
    47641 gacccgtctg catgacgagg cggtttatct gcctatcagc tacatctcga tgatggtggt
    47701 atcaaaaccg gagctgggta atatccccta cgcgccgatc gccaccgaaa ttccgttcga
    47761 acagattaaa ccggtgaaac cttaatgttg cgttacgtat tacgccgctt tctgctgctg
    47821 atcccgatgg tgctcgccgc ctcggtgatc atttttctga tgctgcgcct cggtaccggc
    47881 gacccggcgc tcgattatct gcgtttatct aacctgccgc cgacgccgga gatgctggcc
    47941 tctacccgca ccatgctcgg gctggatcag ccgctgtacg tccagtacgg tacctggttg
    48001 tggaaggcgc tgcatcttga ctttggtatc tcattcgcca gccaacgccc ggtactggac
    48061 gatatgctga acttcctgcc cgccacgctg gaacttgcag gtgcggcgct ggtattaatt
    48121 ctgctcactt ccgtaccgct cggtatctgg gcggcgcgcc accgcgatcg tttgccggat
    48181 ttcgccgtgc gctttatcgc gtttcttggc gtgtcgatgc ccaacttctg gctggcattt
    48241 ttgctagtga tggcgttttc ggtgtatctg caatggctac ccgcgatggg ctacggcagc
    48301 tggcagcaca tcattttacc tgcggtttcc atcgccttta tgtcgctggc gattaacgcg
    48361 cgactgctgc gcgccagtat gctggacgtc gccggtcagc gtcacgtcac ctgggcgcgg
    48421 ctacgcggcc tgaatgacaa acagaccgaa cgccgccata ttctgcgcaa tgcctcgctg
    48481 ccgatgatca ccgccgtggg gatgcacatt ggcgaattga ttggcgggac gatgattatc
    48541 gaaaacatct ttgcctggcc tggcgtcggg cgctatgcgg tgtcggcgat ttttaaccgt
    48601 gactatccgg tgattcagtg ctttacgctg atgatggtgg tggtttttgt ggtctgtaat
    48661 ttgattgtcg atttgctcaa cgccgcgctg gacccgcgca ttcgtcgtca tgaaggagcg
    48721 cacgcgtgaa ctttttcctc tcttcccgct ggtcggtacg cctggcgctg atcattatcg
    48781 ccctgctggc gctgattgcg ctcaccagcc agtggtggca gccgtacgat ccacaggcaa
    48841 ttgatttgcc gtcgtgcctg ctttcgccgg atgcgcagca ctggctgggc accgatcact
    48901 taggtcgcga tattttctcg cggctgatgg cagcgacccg cgtgtcgctc ggttcggtaa
    48961 tggcctgcct gctgctggtg ctgacattag ggctggttat tggcggcagc gccgggttga
    49021 ttggcgggca cgttgatcag gccaccatgc gcgtcgccga tatgtttatg accttcccga
    49081 cctcgattct gtcgttcttt atggttggcg tgctcggcac cgggctgacc aacgtaatta
    49141 tcgccatcgc cctgtcgcac tgggcgtggt atgcacgcat ggtgcgcagc ttggtgattt
    49201 cactacgcca acgcgagttt gtgctggcgt cacggctttc cggtgcgggc catgtgcggg
    49261 tgtttgtcga tcatctggca ggcgcggtga tcccttcgct gctggtgctg gcaacgctgg
    49321 atatcggcca tatgatgctg cacgtcgcgg ggatgtcttt ccttggcctc ggtgtgaccg
    49381 cgccgaccgc cgaatggggc gtgatgatta acgacgcgcg ccagtatatc tggacccagc
    49441 cgctgcaaat gttctggccg gggctggcgc tgtttatcag cgtgatggcc tttaacctgg
    49501 tgggtgacgc actgcgcgat catctggatc ctcatctggt gacggagcac gcacactaat
    49561 gccgcaacag attgaactac gtaatatcac gctacaggcc gcgcagccgc tggtacacgg
    49621 tgtatcgtta accctgcaac gcgggcgcgt gctggcgtta gtcggcggta gcggcagcgg
    49681 gaaatcatta acctgcgccg cgacgctggg cattttgccc gctggcgttc gccagacggc
    49741 gggggaaatt ttagccgatg gcaaaccggt ttcgccttac gccctgcgcg gcatcaaaat
    49801 tgccaccatc atgcagaacc cgcgcagcgc ctttaatcca ctgcacacca tgcacaccca
    49861 cgcgcgggaa acctgcctgg cgttagggaa acccgccgat gacgctacgc ttaccgctgc
    49921 catagaagcg gtggggctgg aaaacgccgc gcgcgtgctg aagctgtacc cgttcgagat
    49981 gagcggcggc atgttgcagc gcatgatgat tgcgatggcg gtgctgtgtg aatcaccgtt
    50041 tatcatcgcc gatgaaccga ccaccgacct cgacgtggta gcacaggcgc gcatcctcga
    50101 tctgctggaa agcattatgc aaaaacaagc gccgggaatg ctgctggtca cccatgatat
    50161 gggcgttgtg gcgcgtctgg cggatgacgt ggcggtgatg tcacaaggta aaattgtcga
    50221 acagggcgat gtagaaacgc tgtttaacgc ccccaaacat acggtgacgc gcagcctggt
    50281 ttccgctcat ctcgccctct acggtatgga gctggcatca tgaccttact taacgtctgc
    50341 ggcctttccc atcactatgc ccacggtgga tttagcggaa aacatcaaca tcaggcggtg
    50401 ctgaataacg tttccctgac cctgaaaagc ggcgaaaccg tcgccctgct gggacgcagc
    50461 ggctgcggaa aaagcaccct cgcgcggctg ctggtaggct tagagtctcc ctcacaaggg
    50521 aatattagct ggcacggcga atcgctggcg aaacttaatc gcgcccaacg taaagccttc
    50581 cgccgtgata ttcagatggt gtttcaggac tccatcagtg ccgtgaatcc gcgcaaaacc
    50641 gtgcgcgaga tcctgcgtga accgatgcgc cacctgctat cactgaaaaa atccgaacaa
    50701 ctggcgcgcg ccagcgaaat gctgaaggcg gtcgatctcg atgacagcgt tctcgacaaa
    50761 cgcccaccgc agttaagcgg cggccagctc cagctcgtct gcctggcacg cgcgctggcg
    50821 gtcgaaccga aattgctgat tctggatgaa gccgtttcta accttgatct cgtgttacag
    50881 gcgggcgtca ttcgcctgct gaaaaagcta caacaacagt ttggcactgc ctgcctgttc
    50941 atcactcatg acttacgcct ggtggaacgc ttttgccagc gagtgatggt gatggacaac
    51001 ggacaaatcg ccgaaaccca ggcggtggga gataaattaa ccttttcctc tgacgccgga
    51061 cgtgtgctac aaaacgcggt attacccgca ttccccgtgc gccgtcgcac ctcagaaaag
    51121 gtttaacgcc aatgcaacga gtcaccatca cgcttgatga cgatttactg gagacgctgg
    51181 acagcctgag ccagcgtcgt ggttataaca accgttccga agctatccgc gatattctgc
    51241 gtagtgccct ggcgcaggaa gctacccagc agcacggcac acaaggtttc gcggtgctgt
    51301 cgtatgtgta tgaacacgaa aaacgcgact tagccagccg tattgtttcc acccagcatc
    51361 atcaccacga cctctccgtc gccacgctac atgtgcacat caaccacgac gactgtctgg
    51421 aaatcgccgt gttgaaaggt gacatgggtg acgtgcagca ttttgccgat gacgttatcg
    51481 cccagcgcgg cgtgcggcac gggcatttgc agtgcctgcc gaaggaagat tgagttattt
    51541 ctaaaacaag gaaag
//
LOCUS       DAZXUK010000005        49830 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZXUK010000005 DAZXUK010000000
VERSION     DAZXUK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49830
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.005"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1224
                     /locus_tag="ACH3L8_000212"
     CDS             <1..1224
                     /locus_tag="ACH3L8_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFT4083507.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="glpX"
                     /locus_tag="ACH3L8_000213"
     CDS             1221..2186
                     /gene="glpX"
                     /locus_tag="ACH3L8_000213"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFT4083508.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="ACH3L8_000214"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="ACH3L8_000214"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFT4083509.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="ACH3L8_000215"
     CDS             2714..3427
                     /locus_tag="ACH3L8_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFT4083510.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="ACH3L8_000216"
     CDS             complement(3399..4076)
                     /locus_tag="ACH3L8_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFT4083511.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="ACH3L8_000217"
     CDS             complement(4070..4747)
                     /locus_tag="ACH3L8_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFT4083512.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5442)
                     /locus_tag="ACH3L8_000218"
                     /pseudo
     CDS             complement(4735..5442)
                     /locus_tag="ACH3L8_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(5443..6021)
                     /locus_tag="ACH3L8_000219"
     CDS             complement(5443..6021)
                     /locus_tag="ACH3L8_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFT4083513.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="ACH3L8_000220"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="ACH3L8_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="ACH3L8_000221"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="ACH3L8_000221"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFT4083514.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="ACH3L8_000222"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="ACH3L8_000222"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFT4083515.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="ACH3L8_000223"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="ACH3L8_000223"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFT4083516.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="ACH3L8_000224"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="ACH3L8_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFT4083517.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="ACH3L8_000225"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="ACH3L8_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFT4083518.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="ACH3L8_000226"
     CDS             12291..13031
                     /locus_tag="ACH3L8_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFT4083519.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="ACH3L8_000227"
     CDS             13198..14148
                     /locus_tag="ACH3L8_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFT4083520.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACH3L8_000228"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACH3L8_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACH3L8_000229"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACH3L8_000229"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFT4083521.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACH3L8_000230"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACH3L8_000230"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFT4083522.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACH3L8_000231"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACH3L8_000231"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFT4083523.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACH3L8_000232"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACH3L8_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFT4083524.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="ACH3L8_000233"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="ACH3L8_000233"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFT4083525.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="ACH3L8_000234"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="ACH3L8_000234"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFT4083526.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="ACH3L8_000235"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="ACH3L8_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFT4083527.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="ACH3L8_000236"
     CDS             23768..23827
                     /locus_tag="ACH3L8_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFT4083528.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="ACH3L8_000237"
     CDS             complement(23928..24530)
                     /locus_tag="ACH3L8_000237"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFT4083529.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="ACH3L8_000238"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACH3L8_000238"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACH3L8_000239"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACH3L8_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFT4083530.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="ACH3L8_000240"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="ACH3L8_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFT4083531.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="ACH3L8_000241"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="ACH3L8_000241"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFT4083532.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="ACH3L8_000242"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="ACH3L8_000242"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFT4083533.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="ACH3L8_000243"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="ACH3L8_000243"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFT4083534.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..29759)
                     /locus_tag="ACH3L8_000244"
     CDS             complement(29676..29759)
                     /locus_tag="ACH3L8_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4083535.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30050..31144
                     /gene="gcvT"
                     /locus_tag="ACH3L8_000245"
     CDS             30050..31144
                     /gene="gcvT"
                     /locus_tag="ACH3L8_000245"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFT4083536.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31168..31557
                     /gene="gcvH"
                     /locus_tag="ACH3L8_000246"
     CDS             31168..31557
                     /gene="gcvH"
                     /locus_tag="ACH3L8_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFT4083537.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31676..34549
                     /gene="gcvP"
                     /locus_tag="ACH3L8_000247"
     CDS             31676..34549
                     /gene="gcvP"
                     /locus_tag="ACH3L8_000247"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFT4083538.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34816..35559
                     /locus_tag="ACH3L8_000248"
     CDS             34816..35559
                     /locus_tag="ACH3L8_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFT4083539.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="ACH3L8_000249"
     CDS             complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="ACH3L8_000249"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFT4083540.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37094..37404
                     /gene="yqfB"
                     /locus_tag="ACH3L8_000250"
                     /pseudo
     CDS             37094..37404
                     /gene="yqfB"
                     /locus_tag="ACH3L8_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
     gene            37568..38227
                     /locus_tag="ACH3L8_000251"
     CDS             37568..38227
                     /locus_tag="ACH3L8_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFT4083541.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="ACH3L8_000252"
     CDS             complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="ACH3L8_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFT4083542.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39646..39912
                     /gene="sdhE"
                     /locus_tag="ACH3L8_000253"
     CDS             39646..39912
                     /gene="sdhE"
                     /locus_tag="ACH3L8_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFT4083543.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39893..40300
                     /locus_tag="ACH3L8_000254"
     CDS             39893..40300
                     /locus_tag="ACH3L8_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFT4083544.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="ACH3L8_000255"
     CDS             complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="ACH3L8_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFT4083545.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40973..41869
                     /gene="xerD"
                     /locus_tag="ACH3L8_000256"
     CDS             40973..41869
                     /gene="xerD"
                     /locus_tag="ACH3L8_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFT4083546.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41894..42604
                     /gene="dsbC"
                     /locus_tag="ACH3L8_000257"
     CDS             41894..42604
                     /gene="dsbC"
                     /locus_tag="ACH3L8_000257"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFT4083547.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42610..44343
                     /gene="recJ"
                     /locus_tag="ACH3L8_000258"
     CDS             42610..44343
                     /gene="recJ"
                     /locus_tag="ACH3L8_000258"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFT4083548.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44434..45532
                     /gene="prfB"
                     /locus_tag="ACH3L8_000259"
     CDS             join(44434..44508,44510..45532)
                     /gene="prfB"
                     /locus_tag="ACH3L8_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFT4083549.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45542..47059
                     /gene="lysS"
                     /locus_tag="ACH3L8_000260"
     CDS             45542..47059
                     /gene="lysS"
                     /locus_tag="ACH3L8_000260"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFT4083550.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47102..47650)
                     /gene="idi"
                     /locus_tag="ACH3L8_000261"
     CDS             complement(47102..47650)
                     /gene="idi"
                     /locus_tag="ACH3L8_000261"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFT4083551.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47705..47776
                     /locus_tag="ACH3L8_000262"
     CDS             47705..47776
                     /locus_tag="ACH3L8_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4083552.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47773..47898)
                     /gene="yqfG"
                     /locus_tag="ACH3L8_000263"
     CDS             complement(47773..47898)
                     /gene="yqfG"
                     /locus_tag="ACH3L8_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFT4083553.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="ACH3L8_000264"
     CDS             complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="ACH3L8_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFT4083554.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12051 a        12452 c        13406 g        11921 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttagtgtt ttttgacatt
    29761 tttttagctc ttaatattgt cttattcaaa ttgactttct catcacatca tctttgtata
    29821 gaaattggtg tattttttgg ttttttattc tgtcgcgatt tttgcatttt ttaaccataa
    29881 gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg
    29941 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt ttgtggtgat
    30001 aatttcacca tgaaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    30061 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    30121 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    30181 cggaatgttt gatgtgtcgc atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    30241 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    30301 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt
    30361 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    30421 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    30481 gattgccgtg caagggccga atgctcaggc aaaagctgcc acactgttta atgatgctca
    30541 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggtgggcg atctgtttat
    30601 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    30661 ggccgatttc tggcgtgcgc tggtggaagc gggcgttaag ccctgcggtc tgggggcgcg
    30721 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    30781 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccgacag atcgtgactt
    30841 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    30901 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    30961 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    31021 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    31081 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    31141 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    31201 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    31261 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    31321 gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat
    31381 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg
    31441 gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc
    31501 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga tgagtaacgg
    31561 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    31621 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    31681 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    31741 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca caatcgttaa acgcgctgac
    31801 cggccagatt gtgccgaaag atattcagct tgcgacacca ccgcaggttg gcgcaccggc
    31861 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    31921 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    31981 gctggaaaat ccgggctggt ataccgcgta cactccgtat cagcctgaag tctcccaggg
    32041 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    32101 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    32161 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccaatg tgcatccgca
    32221 aacgctggat gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga
    32281 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    32341 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    32401 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    32461 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    32521 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    32581 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    32641 gcaacatatt cgtcgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    32701 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    32761 ccgcattcac cgtctgaccg atattctggc ggcgggcctg caacaaaaag gtctgaaact
    32821 gcgccatgcg cactatttcg acaccttgtg tgtggaagtg gccgacaaag cgggcgtact
    32881 gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg ccgtggggat
    32941 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagtg tgctgctggg
    33001 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    33061 tatccagcct gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta
    33121 tcacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    33181 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    33241 gatcccaatc acttggacgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    33301 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    33361 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    33421 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    33481 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    33541 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    33601 gggcgataac ctctcctgta tcatggtgac ttatccttct acccacggcg tgtatgaaga
    33661 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    33721 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    33781 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    33841 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    33901 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    33961 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    34021 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    34081 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    34141 gaaagaagaa accggcatca gcgagctgga cattgccaag cgcctgatcg actacggttt
    34201 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    34261 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    34321 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    34381 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    34441 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    34501 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc
    34561 gagtattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    34621 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    34681 gcgcgaatat ttaccctcac cccgaccctc tccctgaaag ggcgaggggg aaaagcgtgc
    34741 caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattaattt
    34801 ttctcggaaa aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg
    34861 gcaactgcat tactgttggc gcaagaaggg tatacggtgg cggttaatta tcagcaaaac
    34921 ctccacgcgg cgcaggaagt gatgaactta ataatgcaag ccggtggcaa agcattcgtg
    34981 ctccaggcgg atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag
    35041 cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc
    35101 gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat
    35161 tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc
    35221 gctatcgtca atgtctcttc ggtggcctca cggttgggtt cgccagggga atatgttgat
    35281 tacgcggcat cgaaaggggc gattgattcg ttaaccaccg gactatcgct ggaagtcgcc
    35341 gcgcagggga tccgcgttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc
    35401 agcggcggcg agcctggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt
    35461 ggacaggcag aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac
    35521 gtcacgggaa gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg
    35581 gatgcaacaa gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca
    35641 atcacctctt tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg
    35701 tcgtcatgtt tattcacata gataaagccg tagcgtttgc tgtactgccc ggtagtgaat
    35761 gacacgcagt cgatgcagcc ccacggtgtg tagcccatca gatccacacc atcgtaagtc
    35821 actgcttttt tcatctcttc aatatgggcg cgcaggtagt caatgcggta gtcgtcgttg
    35881 atgctgccat cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc
    35941 ggcctctgat aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc
    36001 tgccagcccc agtcagacgc tttaacatac gggttcggta cgctgccttc aaaaccagag
    36061 atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca
    36121 agataatcgc aggtgccttc gcgcagtaca tcgagatcgc cgtcttccat tttgatatta
    36181 aatccgcgac gttcccactc gttcaataca taggacgggt aatagccgca tagctgcaca
    36241 tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta
    36301 catgagtagg gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta
    36361 atgcgacgcg cggctttcac tgccagggcg ctggcgacaa actggtgatg cagcacctgg
    36421 tacatcgtct cttccgggtt gtcatgctcg gtatacacca cgccggagca gcagtaacca
    36481 aacagcggcg cacgccagtt acgctggttg tttatttcgt tgaaggtcat ccagtatttg
    36541 actttatgct tgtagcgttc gaataccact tccgcgaaac ggacaaagaa atcaacgaat
    36601 ttacggttgg tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag
    36661 agggttatca ccggttcgat gttgtatttc agcagttcat cgaacagaga gtcgtaaaac
    36721 ttcagccctt cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg
    36781 atggaagtcc gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag
    36841 tgaccataaa aatcaacggc ttcatggttt ggatagtatt ttcctggcaa gacttctttg
    36901 gtgatttcgc gcggcacgcc gtgtgcgcca caggtcagaa cgtcacaaat gctcggtcct
    36961 ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg caacggcgcc accccataag
    37021 aaatctttcg gtaaggtgag ttttttcact atcataggct ccagatcagt caattcttac
    37081 aggaaatagt accatgcagc caaacgacat cacttttttc aacgtttcca ggatgacatt
    37141 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    37201 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    37261 gcaacctcaa ccgtaacgct ggatacactg acggaaaaac atgcagagca ggaaaatatg
    37321 accctgcctg aactgaaaaa ggtcattgct gacatctatc ccggtcagac acaattttat
    37381 atgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttcgcattt
    37441 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    37501 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctctgga
    37561 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    37621 caacagcgtc agtcacggca ttgggctggt gtttggtatc gttgggctgg tgttgctact
    37681 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagttaca gcctctatgg
    37741 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    37801 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    37861 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    37921 tatctggagc ctagcattgc tggggattct gttcaaactg accatcgcgc accgattcaa
    37981 aattttatct ctggtgactt atctggcgat gggctggctg tcgctggtgg taatttatga
    38041 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    38101 ttcgctcggg gtgattttct acgtctgcaa acgcattcca tacaaccatg ccatctggca
    38161 cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg atctatttgt atattgggca
    38221 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgt cagcgtcgca tcaggcatcg
    38281 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    38341 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    38401 atgcctgatg cgatacagag atttactctt cgagcgaata cggcagcggc tcgatatgca
    38461 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    38521 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    38581 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    38641 cactacctgc cagcaaccag agcgcgcgtt tgttggcacc acggaatttt gctcgcgcca
    38701 ccatctcttg tccggtataa cagcctttct taaagctgat accgcccagc gcctggagat
    38761 tggtcgcctg tgggataaac tgcccgctgt tggcggcatc aatcaccggg aaaccagctt
    38821 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    38881 cggtcagcat attagcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    38941 caaaccatag caaagtggtc gcgccttctt tgactacctg tttttctttc gaaggcagtt
    39001 cgctaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    39061 gctcgtcgtc tggcgcgatg gtcactttag agaataccgc atattttttc agttcagtca
    39121 gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca
    39181 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    39241 cttctgccat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    39301 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    39361 cagaagccgt aggctgacgt ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    39421 cttaacggcg tattaatgac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    39481 tacgcgatct gtgccttctg cgaggcgact gccaaaaaaa tgaagccctt gaattgttgt
    39541 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    39601 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    39661 aaagcccgaa ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    39721 ccgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    39781 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    39841 gatgcagaac tggaaataat ggtccgactc atccagacac ggaaccggga acgtggtcct
    39901 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    39961 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    40021 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggtgta ttaatgctcg
    40081 ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    40141 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    40201 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagccg aatggcggga
    40261 tttacggcgg attttgttgc aacaagagac gcaaagataa agatgacggc aaagcatatg
    40321 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    40381 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    40441 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    40501 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    40561 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaataa
    40621 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    40681 tttcaccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    40741 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    40801 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    40861 taacgtaagc ttctcgattc tgctgtggct tttgcagcta gtgtatcaga atcgctttag
    40921 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    40981 ggagctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    41041 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    41101 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    41161 ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    41221 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    41281 gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    41341 ggcgccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    41401 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctgcg
    41461 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttagggga
    41521 agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgctgaatgg
    41581 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    41641 ctggcaccgc attaaacatt atgccgtgct ggcgggtatc gacagcgaaa agctgtcacc
    41701 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    41761 ggtgcagatg ctactgggcc acagcgatct ttccaccacg caaatttata cgcatgtcgc
    41821 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    41881 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    41941 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcatcaaaag
    42001 cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta acagcggtgt
    42061 gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    42121 cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg cgcttgaaaa
    42181 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    42241 tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg cgctggggat
    42301 caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag agaaagaaat
    42361 gaaagctatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggcaggtaa
    42421 aagcgtcgca ccagccagct gcgacgtgga tattgccgac cattacgcac ttggcgtcca
    42481 gcttggcgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg ttccgggtta
    42541 ccagccgccg aaagacatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    42601 ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    42661 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    42721 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    42781 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    42841 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    42901 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    42961 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    43021 aacggtattt cctcccatgc gggagttgag cacgctcgct cgttgggcat cccggttatt
    43081 gttaccgatc accatttgcc gggcgacaca ttacccgcag cggaagcgat cattaaccct
    43141 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    43201 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    43261 gcaattccta acctggcaga actgctggat ctggtagcac tggggacagt agcggacgtc
    43321 gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    43381 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaatcgcga tgcacaaaaa
    43441 ctagccgcca gcgatttagg ttttgcgctg ggaccgcgtc tcaatgctgc cgggcgactg
    43501 gatgatatgt ccgttggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    43561 ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag agatcgaaca aggaatgcag
    43621 gttgaagccc tgaccctgtg cgagaaactg gagcgcagcc gtgacacgct acccggcggg
    43681 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgtatc
    43741 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgacgg tacgctgaaa
    43801 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    43861 ctctaccccg gcatgatgct caagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    43921 gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac tgagtggctg
    43981 gccccttcgc tattgcaagg cgaagtggtg tcagacggtc cgttaagccc ggccgaaatg
    44041 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    44101 ccgctgtttg acggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    44161 aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg atggtattgc ttttaatgtc
    44221 gataccgctc tctggccgga taacggcgtg cgcgaagtgc aactggctta taagctcgat
    44281 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    44341 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    44401 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    44461 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    44521 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    44581 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    44641 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    44701 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    44761 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgtta
    44821 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    44881 gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    44941 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    45001 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc cgtttgactc
    45061 cggcggtcgt cgccacacgt cgttcagctc tgcgtttgtt tatccggaag ttgatgatga
    45121 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    45181 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    45241 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aacaaagatc aggccatgaa
    45301 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgccg agaaacaggc
    45361 gatggaagat aacaaatctg acatcggctg gggcagccag attcgttctt atgtccttga
    45421 tgactcccgc attaaagatc tgcgcaccgg ggtagaaacc cgcaacacgc aggccgtgct
    45481 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    45541 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    45601 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    45661 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    45721 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    45781 gggtaaagcg tctttcgtta ccctgcagga tgtgggtggc cgcattcagc tgtacgttgc
    45841 ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg acctcggcga
    45901 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    45961 taccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat tccacggctt
    46021 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    46081 ccgtaacacc tttaaagtac gctcgcagat cctctctggt attcgccagt tcatggtgaa
    46141 ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    46201 gcgtccgttt atcacccacc ataacgcgct ggatctcgac atgtacctgc gtatcgcgcc
    46261 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    46321 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    46381 ctacatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    46441 tctggcacag gatattctcg gtaagacgga agtgacctac ggcgacgtga cgctggactt
    46501 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    46561 cgacatggcg gatctggata acttcgactc tgcgaaagcg attgctgaat ctatcggtat
    46621 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    46681 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    46741 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    46801 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcaacgctt
    46861 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgatgaaga
    46921 ttacgttacc gcactggaac atggcttacc gccgacagca ggtctgggga ttggcatcga
    46981 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    47041 gatgcgtccg gtgaaataag cattacgtta tgctcacaac cccggcaaat gtcggggttt
    47101 tttatttaag ctgggtaaat gcagataatc gtattctggc ttcgcgattt gtcgcctgca
    47161 tcaccatcca cggactgaac gcccacggcg tggcatcaat accgcgtaat acatcagcta
    47221 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctggtggtgc
    47281 gtgcggcaaa taccggacac acttcatttt ccacaatgcc tctcggatcg gtggcgcggt
    47341 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    47401 aacggcggat caccgcgtct tcgctgcttt ctcccagttg tgggtgccca caaaccgagt
    47461 tagtccacac gccaggccac gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    47521 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    47581 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    47641 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    47701 tgaaatgatt aaccaagaga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    47761 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    47821 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacaacagac tgaatatagc
    47881 gcgcattaaa aaattcatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    47941 gccacatacg tactgtcctg acttctaaat ctttatcaga gacggattcc ttcaccaggt
    48001 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    48061 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    48121 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    48181 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    48241 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    48301 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    48361 tatgaacgcg catcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    48421 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg caaataatat
    48481 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    48541 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg
    48601 gcataccaaa cgacatcggg gtgacaatcg caaaccacga ggcatcatgt agcccggata
    48661 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    48721 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    48781 caaaggagat acctaaataa acgggattac catattgcgg attcccttta cctccagcgg
    48841 cccagtcaat acccacctga atgatgctaa gcccaataga agtaataacc acaccggtaa
    48901 ccagtggcgg gaataaaggc atcaagcgac caataagtgg cgctaataat gtggtgataa
    48961 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc gatatccggg ttcatcccaa
    49021 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    49081 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    49141 ccgagctaat gagcatcgca atagcatctt ttgagaggcc cagtcggtca ccaatcataa
    49201 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    49261 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    49321 agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagaataaaa
    49381 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    49441 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg cttaaaccac
    49501 gaaaatgaca agagaaactg acaatggaat tatctgtaat tttctcatat tgatatttca
    49561 ttaatcattc tggcatgtcg cactctcgca tttaatcgtt tttatctgga tagcgctctt
    49621 ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata atttgaactc
    49681 agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt tattgcatcg
    49741 cgaaatttga gaagacataa ctcattaaat gaaagggatg caaatgaata cgtttatcgc
    49801 tgctgaagct gcggaatgta taggctgtca
//
LOCUS       DAZXUK010000006        46752 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZXUK010000006 DAZXUK010000000
VERSION     DAZXUK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46752
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.006"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..371)
                     /locus_tag="ACH3L8_000265"
     CDS             complement(<1..371)
                     /locus_tag="ACH3L8_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFT4083555.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEV"
     gene            complement(476..1246)
                     /locus_tag="ACH3L8_000266"
     CDS             complement(476..1246)
                     /locus_tag="ACH3L8_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4083556.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(1243..2169)
                     /locus_tag="ACH3L8_000267"
     CDS             complement(1243..2169)
                     /locus_tag="ACH3L8_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFT4083557.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            2278..2940
                     /gene="can"
                     /locus_tag="ACH3L8_000268"
     CDS             2278..2940
                     /gene="can"
                     /locus_tag="ACH3L8_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFT4083558.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(2981..3517)
                     /gene="hpt"
                     /locus_tag="ACH3L8_000269"
     CDS             complement(2981..3517)
                     /gene="hpt"
                     /locus_tag="ACH3L8_000269"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFT4083559.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            3723..6113
                     /gene="gcd"
                     /locus_tag="ACH3L8_000270"
     CDS             3723..6113
                     /gene="gcd"
                     /locus_tag="ACH3L8_000270"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFT4083560.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(6160..7710)
                     /gene="cueO"
                     /locus_tag="ACH3L8_000271"
     CDS             complement(6160..7710)
                     /gene="cueO"
                     /locus_tag="ACH3L8_000271"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFT4083561.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            7876..8223
                     /locus_tag="ACH3L8_000272"
     CDS             7876..8223
                     /locus_tag="ACH3L8_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFT4083562.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            8329..9195
                     /gene="speE"
                     /locus_tag="ACH3L8_000273"
     CDS             8329..9195
                     /gene="speE"
                     /locus_tag="ACH3L8_000273"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFT4083563.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            9211..10005
                     /gene="speD"
                     /locus_tag="ACH3L8_000274"
     CDS             9211..10005
                     /gene="speD"
                     /locus_tag="ACH3L8_000274"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFT4083564.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(10043..10405)
                     /gene="yacL"
                     /locus_tag="ACH3L8_000275"
     CDS             complement(10043..10405)
                     /gene="yacL"
                     /locus_tag="ACH3L8_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFT4083565.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(10580..13177)
                     /gene="acnB"
                     /locus_tag="ACH3L8_000276"
     CDS             complement(10580..13177)
                     /gene="acnB"
                     /locus_tag="ACH3L8_000276"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFT4083566.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            13532..15385
                     /locus_tag="ACH3L8_000277"
                     /pseudo
     CDS             13532..15385
                     /locus_tag="ACH3L8_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(15456..16880)
                     /gene="lpdA"
                     /locus_tag="ACH3L8_000278"
     CDS             complement(15456..16880)
                     /gene="lpdA"
                     /locus_tag="ACH3L8_000278"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFT4083567.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(17205..19085)
                     /gene="aceF"
                     /locus_tag="ACH3L8_000279"
     CDS             complement(17205..19085)
                     /gene="aceF"
                     /locus_tag="ACH3L8_000279"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFT4083568.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(19100..21763)
                     /gene="aceE"
                     /locus_tag="ACH3L8_000280"
     CDS             complement(19100..21763)
                     /gene="aceE"
                     /locus_tag="ACH3L8_000280"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFT4083569.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(21924..22688)
                     /gene="pdhR"
                     /locus_tag="ACH3L8_000281"
     CDS             complement(21924..22688)
                     /gene="pdhR"
                     /locus_tag="ACH3L8_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFT4083570.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            23229..24602
                     /gene="aroP"
                     /locus_tag="ACH3L8_000282"
     CDS             23229..24602
                     /gene="aroP"
                     /locus_tag="ACH3L8_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFT4083571.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(24645..25499)
                     /gene="ampE"
                     /locus_tag="ACH3L8_000283"
     CDS             complement(24645..25499)
                     /gene="ampE"
                     /locus_tag="ACH3L8_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFT4083572.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(25496..26047)
                     /gene="ampD"
                     /locus_tag="ACH3L8_000284"
     CDS             complement(25496..26047)
                     /gene="ampD"
                     /locus_tag="ACH3L8_000284"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFT4083573.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            26135..27028
                     /gene="nadC"
                     /locus_tag="ACH3L8_000285"
     CDS             26135..27028
                     /gene="nadC"
                     /locus_tag="ACH3L8_000285"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFT4083574.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            27231..27671
                     /gene="ppdD"
                     /locus_tag="ACH3L8_000286"
     CDS             27231..27671
                     /gene="ppdD"
                     /locus_tag="ACH3L8_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFT4083575.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            27681..28828
                     /locus_tag="ACH3L8_000287"
                     /pseudo
     CDS             27681..28828
                     /locus_tag="ACH3L8_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            28818..30020
                     /gene="hofC"
                     /locus_tag="ACH3L8_000288"
     CDS             28818..30020
                     /gene="hofC"
                     /locus_tag="ACH3L8_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFT4083576.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(30055..31098)
                     /gene="guaC"
                     /locus_tag="ACH3L8_000289"
     CDS             complement(30055..31098)
                     /gene="guaC"
                     /locus_tag="ACH3L8_000289"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFT4083577.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(31260..31298)
                     /locus_tag="ACH3L8_000290"
                     /pseudo
     CDS             complement(31260..31298)
                     /locus_tag="ACH3L8_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            31323..31943
                     /gene="coaE"
                     /locus_tag="ACH3L8_000291"
     CDS             31323..31943
                     /gene="coaE"
                     /locus_tag="ACH3L8_000291"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFT4083578.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            31943..32686
                     /gene="zapD"
                     /locus_tag="ACH3L8_000292"
     CDS             31943..32686
                     /gene="zapD"
                     /locus_tag="ACH3L8_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFT4083579.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            32696..32893
                     /gene="yacG"
                     /locus_tag="ACH3L8_000293"
     CDS             32696..32893
                     /gene="yacG"
                     /locus_tag="ACH3L8_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFT4083580.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(32993..33382)
                     /gene="mutT"
                     /locus_tag="ACH3L8_000294"
     CDS             complement(32993..33382)
                     /gene="mutT"
                     /locus_tag="ACH3L8_000294"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFT4083581.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(33442..36147)
                     /gene="secA"
                     /locus_tag="ACH3L8_000295"
     CDS             complement(33442..36147)
                     /gene="secA"
                     /locus_tag="ACH3L8_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFT4083582.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(36209..36721)
                     /gene="secM"
                     /locus_tag="ACH3L8_000296"
     CDS             complement(36209..36721)
                     /gene="secM"
                     /locus_tag="ACH3L8_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFT4083583.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(36952..37869)
                     /gene="lpxC"
                     /locus_tag="ACH3L8_000297"
     CDS             complement(36952..37869)
                     /gene="lpxC"
                     /locus_tag="ACH3L8_000297"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFT4083584.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(37970..39121)
                     /gene="ftsZ"
                     /locus_tag="ACH3L8_000298"
     CDS             complement(37970..39121)
                     /gene="ftsZ"
                     /locus_tag="ACH3L8_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFT4083585.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(39182..40444)
                     /gene="ftsA"
                     /locus_tag="ACH3L8_000299"
     CDS             complement(39182..40444)
                     /gene="ftsA"
                     /locus_tag="ACH3L8_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFT4083586.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(40441..41271)
                     /gene="ftsQ"
                     /locus_tag="ACH3L8_000300"
     CDS             complement(40441..41271)
                     /gene="ftsQ"
                     /locus_tag="ACH3L8_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFT4083587.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(41273..42193)
                     /gene="ddlB"
                     /locus_tag="ACH3L8_000301"
     CDS             complement(41273..42193)
                     /gene="ddlB"
                     /locus_tag="ACH3L8_000301"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFT4083588.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(42186..43661)
                     /gene="murC"
                     /locus_tag="ACH3L8_000302"
     CDS             complement(42186..43661)
                     /gene="murC"
                     /locus_tag="ACH3L8_000302"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFT4083589.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(43715..44782)
                     /gene="murG"
                     /locus_tag="ACH3L8_000303"
     CDS             complement(43715..44782)
                     /gene="murG"
                     /locus_tag="ACH3L8_000303"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFT4083590.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(44779..46023)
                     /gene="ftsW"
                     /locus_tag="ACH3L8_000304"
     CDS             complement(44779..46023)
                     /gene="ftsW"
                     /locus_tag="ACH3L8_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFT4083591.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(46023..>46752)
                     /gene="murD"
                     /locus_tag="ACH3L8_000305"
     CDS             complement(46023..>46752)
                     /gene="murD"
                     /locus_tag="ACH3L8_000305"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFT4083592.1"
                     /translation="AAKLRIYENAKVCVVNADDALTMPIRGADERCVSFGVNMGDYHL
                     NHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVALALADAAGLPRASSLKALTTFT
                     GLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGLHVDGTLHLLLGGDGKSADFSPL
                     VRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETMEQAMRLLAPRVQPGDMVLLSPAC
                     ASLDQFKNFEQRGNEFARLAKELG"
BASE COUNT        11141 a        12966 c        11484 g        11161 t
ORIGIN      
        1 acctccggcc cacccagttc gacaatacac tcacgaaact ctgaactggt catggtttca
       61 cggcaagcca tcatctgttc aattaacggt aacgtgacac cagaaatcac ttcgcaacgg
      121 gagtgtttgt ggcttaataa tgaagccacg cgatacggtg gcgctccggc tatatcggtt
      181 aagaagatga caccctcacc agagtccgct tcatgcagag catcgcacat catgcggctg
      241 agcatattag agcttaatcc gcgccagaaa ttcacggccc ggcactgaag aagtgcccca
      301 tgttttttct ccagcgcatc cagtatctct tgcgcccggt catcgtgaca ggtaattacc
      361 caacctaaca tcgccttcct ccttagccga aaatcagcct accctgacca ttgttgataa
      421 agggtgatag ccgtcaaaac tcctctcccc cgaatccaga ggagaggaaa tagccttagc
      481 tacgcaaacc acgtccacgt tggatcagcg accaacagat caaataaaac gccacaataa
      541 agaccaccag tacgccaaag gtagtgacca gcggcacatc atttataccg aggaagccgt
      601 agcggaaacc actgatcata taaacgattg ggttcaggtg cgacagccct tgccagaacg
      661 gcggcaacaa agtcagtgag taaaagaccc cgcccaaata cgtgagtggc gttaacacaa
      721 aggttggcac caggctgatg tcatcgaacg ttttggcaaa cacgccgttc agcaaacccg
      781 caagggagaa tagcaccgcc gtgagcacca gggttaaggc aacgaatacc cacgaatgca
      841 cctgaaacgg cacaaaaaac agtgaaattg ccgtcacaag aatgccaaca aacagaccac
      901 gcgccacgcc accgccgaca tatccggcaa taatgacgtg agtcggaacc ggcgctacca
      961 gcagctcttc aatattacgc tggaacttgg caccaaaaaa tgatgacgcg acgttagcgt
     1021 aggcgttggt gatcaccgac atcatgatca gccccggtac gatgaactgc atatagctga
     1081 agccatgcat atcgccaata cgcgaaccta tcaggttacc gaagataata aagtaaaggg
     1141 tcatggtgat gactggcggc accagcgtct gcacccagat acgcataaag cgatggatct
     1201 ctttcgccca gatgcttttt agcgccaccc agtaaagatg catcatgcgc gatctccttg
     1261 tttttcatta accagtgaaa caaacagctc ttccagacgg ttagctttgt tacgcatact
     1321 taatacctga atgccctgct cacttaactg cgtaaatacg ctgttgatcc cctgctcacg
     1381 tagcacttca acttccagcg tcgcggtatc gaccagtcga tactgatagc catcgagctt
     1441 cggtaacgga cttttcggtg cgagatcgag aataaaggtt tccgatttca gcttcgccag
     1501 cagcgccttc atcgaggtat tttccaccag ctcaccatgt tgaataatgc cgatattgcg
     1561 gcacagcatt tctgcttctt ccaggtagtg tgtggtaaga atgatggtgg tgcctttgtc
     1621 gtttaaatcc ttcaaaaagc cccacattga gcggcgaagt tcaatatcca cgcctgcggt
     1681 cggttcgtcg agaatcagta gtttaggttc atgcattaac gcacgggcaa tcattaaacg
     1741 gcgcttcatc ccgccagata acatacgcgc acgttcgttg cgttttcccc ataaatcgag
     1801 ctgcttaaga tatttttcgc tgcggatgta cgcctctttg cgctccacac cgtagtaccc
     1861 tgcctgattc accacaattt gctgcacggt ttcaaacggg ttgaagttaa attcctgcgg
     1921 caccagtccc aactgacgtt tagcgttcac gacatctttc tcgagatcgt aaccaaatac
     1981 gctgacccgc ccggaggttt tatttaccag agagctgata ataccgatag tggtcgattt
     2041 cccggccccg ttcggcccga gaagcgcata aaaatcaccc gcttcgacct gcaaatctat
     2101 tccacgaagc gcctgaacgc cgcctggata gtttttttta agctgttgaa gttccagtgc
     2161 aatggtcata aatttttact taccttacgt tcttacactt tatctgtggt ttaaatcgtc
     2221 ccggagttgc cctatattag ccaaacgtaa ttatttggtt acaggtcgtt aacctccatg
     2281 aaagacatag atacactcat cagcaacaat gcactatggt caaaaatgct ggtggaagag
     2341 gatcccgggt tttttgagaa actggcacaa gcgcaaaaac cgcgctttct atggattgga
     2401 tgttccgaca gtcgcgttcc tgcagaacgt ttaaccggtc ttgagccggg cgaactcttt
     2461 gttcaccgta atgttgctaa cctagtcatt cacaccgacc tgaactgcct ttccgtggtt
     2521 cagtatgcag tggatgtact cgaagttgaa cacattatta tctgtggcca ctacggttgc
     2581 ggcggcgtac aagccgccgt tgaaaacccg gaactggggc ttatcaacaa ctggctgctg
     2641 catatccgcg atatctggtt caaacatagc tcattgctcg gcgaaatgcc gcaagagcgc
     2701 cgtctggata ccttgtgtga actgaacgtc atggaacagg tgtataacct gggccactcc
     2761 accattatgc aatcagcgtg gaaacgcggg cagaaagtta ccattcacgg ctgggcctac
     2821 ggcattcacg acggcttgct gcgtgatctg gatgttaccg ccaccaaccg cgaaaccctt
     2881 gagcaacgtt accgtcacgg gatttccaac ctcaagctga aacacgccaa ccacaaataa
     2941 aaatgccatg ccggatgcaa cacatccggc aacttcacac ttactcgtcc agcagaatca
     3001 ctttgccaat atacggcaga tgacggtaac gctgtgcgta atcaatgccg taacccacca
     3061 caaactcatc cgggatcgag aaaccgataa attcaaccgg gacgttcact tcacgacggg
     3121 acggtttatc cagcagcgta caaatcgcca acgacttcgg ttcgcgcagg cttaagatct
     3181 cacgcacttt cgacagtgta ttccccgagt cgatgatatc ttcaacaatc agcacgtcct
     3241 tgccacggat atcttcatcc agatctttga ggattttcac atcacgggtg gtggacatgc
     3301 cgctaccgta gctggaggcg gtcataaagt cgacttcatg agatacctga acttcacggc
     3361 acaggtccgc cataaacata aatgagccac gcagcagacc caccagcacc atatcgctgc
     3421 cgctgtcttt gtaacgctca gtaatctgac gacccagttc ggcgatacgc gctttaatct
     3481 ccgcttcggg gatcattact tctacagtat gtttcatatc tctaaccata tgatttaaaa
     3541 acaaatcact tcgcacgggc aggttttggc accgcaacaa agcgcaagac gcgcagtata
     3601 ccagtaaaac tattcattcg cggaatctgt taataaagct cgaattgtga tgacggtcac
     3661 acatgttaaa acctataatt aattgctact aaaatattaa tgaattgaaa tggtgtctct
     3721 ttatggcaat taacaataca gcctcgcgac gattactcgt cacgctaaca gccctttttg
     3781 cagcgctttg cgggctgtat ctactcattg gcggaggctg gctggtcgcg attggcggct
     3841 cctggtacta ccctatcgct ggccttgtga tgctcggcgt cgcctggatg ctgtggcgca
     3901 gtaaacgcgc cgcgctttgg ctatacgcag ccctgctgct cggcaccatg atttggggcg
     3961 tctgggaagt tggtttcgac ttctgggcgc tgactccgcg cagcgatatt ctggtcttct
     4021 tcggcatctg gctgatcctg ccgtttgtct ggcgtcgcct ggtcattcct gccagcggcg
     4081 cagttgccgc actggtggtc gcactgctga ttagcggtgg tatcctgacc tgggccggat
     4141 ttaacgatcc gcaggagatc aacggcacct taagcgccga tgccacacct gccgaagcta
     4201 tctcccccgt tgccgatcag gactggcctg cgtatggccg caatcaggaa ggtcaacgct
     4261 tttcaccact gaagcaaatt cacgccgata acgtccacaa gctgaaagaa gcctgggtgt
     4321 tccgtactgg cgatgtgaag cagccgaacg atccgggtga aatcaccaat gaagtgacgc
     4381 cgattaaagt gggcgatacc ctttacctgt gtaccgctca ccagcgcctg tttgcgcttg
     4441 atgccgccag cggcaaagag aaatggcatt acgatcctga gctgaaaacc aacgagtctt
     4501 tccagcacgt aacctgccgt ggcgtctctt atcatgaagc caaagcagaa acggcttcgc
     4561 cggaagtgat ggcggattgc ccgcgtcgta tcattcttcc agtgaatgat ggtcgcctga
     4621 ttgcgattaa cgctgaaaac ggcaaactgt gcgaaacctt cgccaataaa ggcgtgctca
     4681 atctgcaaag caatatgcca gacaccaaac cgggtctgta tgagccgact tctccgccaa
     4741 ttatcaccga taaaaccatc gtgatggccg gttcagtcac cgataacttc tcaacccgcg
     4801 aaacgtctgg cgtgatccgt ggttttgatg tcaacaccgg ggagctgctg tgggcttttg
     4861 atccgggcgc gaaagatccg aacgcaatcc cgtcagacga acacaccttt acctttaact
     4921 caccaaactc gtgggcacca gcggcctatg acgcgaagct ggatctggtc tatctgccga
     4981 tgggcgtgac cacgccggat atctggggcg gtaaccgcac accggaacag gaacgttatg
     5041 ccagctcgat tctggcgctg aatgccacta ccgggaaact ggcgtggagc taccagaccg
     5101 ttcaccacga cctgtgggac atggatcttc cggcacagcc gacgctggcg gacatcaccg
     5161 ttaatggtca gaaagtgccg attatttacg ctccggcgaa aaccggtaac atttttgtgc
     5221 tcgatcgtcg taatggcgaa ctggtggttc cggccccgga aaaaccggtt ccccaaggtg
     5281 ctgccaaagg cgattacgtt acccctactc aaccattctc ggaactgagc ttccgtccga
     5341 cgaaagattt gagcggtgcg gatatgtggg gagccaccat gtttgaccaa ctggtgtgcc
     5401 gcgtgatgtt ccaccagatg cgctatgaag gcattttcac gccgccatct gaacagggta
     5461 cgctggtctt cccgggtaac ctggggatgt tcgaatgggg cgggatttcc gttgatccaa
     5521 atcgtgaagt ggcaattgcc aacccaatgg cactgccgtt tgtttcgaaa ctgctcccgc
     5581 gtggtcctgg caacccgatg gagcagccga aagatgccaa aggcacgggt acggaatccg
     5641 gcattcagcc acagtacggt gtaccgtatg gtgtcacgct caacccgttc ctctcaccat
     5701 ttggtctgcc atgtaaacag ccagcatggg gttatatctc ggcgctggat ctgaaaacta
     5761 atgaagtggt gtggaagaaa cgtattggta cgccgcagga cagtatgccg ttcccgatgc
     5821 cggttccggt gccgttcaat atgggtatgc cgatgctggg cgggccaatc tccacggcgg
     5881 gtaacgtgct gtttatcgcc gctacggcag ataactacct gcgcgcttac aacatgagca
     5941 acggtgaaaa actgtggcag ggtcgtttac cggcgggtgg tcaggcaaca ccgatgacct
     6001 atgaagtgaa tggcaagcag tacgtggtga tctccgcagg cggtcacggt tcatttggta
     6061 cgaagatggg cgactatatt gtggcttatg ccctgccgga tgatgtgaaa taaccataat
     6121 gcccggagag atccgggcat atttccgaat acggtctttt tataccgtaa accctaacat
     6181 catccccgta tcttcatgct ccagcagatg gcagtgcgcc atataagcat gttctttcgg
     6241 tgcgttgtga ttaaacttca ccagcacttc actgacattg ccttctacct taacggtatc
     6301 tttccagccc gcgcgatgag ccgctggcgg tttgccattt tctgacaaga tacggaactg
     6361 cgtgccgtga atatggaacg gatgcagcat catgtcgccc acgccagaga taacccaacg
     6421 ttcgtactgc cctttcgccg ccgcaaacat cggcttattc atatcaaacg cctgaccgtt
     6481 gattttattg gcatggtgga aatcgaactt cccgccgtgg ttcatatgat tcatattgcc
     6541 gtgccccata tggcccatca tctggctgtg atccattccg gccatcgcct gatcgccata
     6601 tttctccatt agcatctgca tccccatcat atcgagcatc gggtccatag aaagttgcag
     6661 cttgcgtacc gtcagccctt ccagcgaagg taacgcaggc aggctactta atgtgtcagg
     6721 caaagcaccg gaggcactaa tagcaatcgg ctgaatccgc attaccggat gcggcttgtc
     6781 aaacggtgca atcgccatcc ccatctggct gaccggcagc gtcaccaggt caaagggttt
     6841 gttgtcgtta acctccacca gcacttcaaa acgctcgccc atcagcaccg gcagctcgct
     6901 caccttcacc ggttcaggta gcagaccacc gtcgctggca atcacataca gcgggcgatt
     6961 gtcgctggtg gcgaaattga gcgagcgggc attacagcca ttgagcaaac gcaggcgcag
     7021 ccaaccacgc ggggcagcgt gttgtgggta gattgcaccg ttggtcagca gcgtatcgcc
     7081 aaaccagccc acggcggcgg tcatcacatc cagttgataa tcaatctgcc cgtcggcgct
     7141 aaatttctta tcctgaacga tcaccggaac atcatcgata ccccactgtt ttggcagcat
     7201 taatttcagg atctcgtcat cttcaatcac caccagacca gccagcccca tcgccacctg
     7261 tcgcccggtc ttgccatgtt gatgtggatg gaaccagcag gtagcggcag gttgatcaac
     7321 gttcaacgtc accgagcgct tgccacctgg cggaataatt ccctgtgggc cgccgtcgac
     7381 gtcacccggt acttccagcc cgtgccagtg caacgtcgtc tcttccgcca gttggttgta
     7441 gatatcaacc gttaccgctt tgccgcgctg taatttcacc gccggcccca gcagattgcc
     7501 gttatagccc caggtagttg cagttttctc gccaaaggtg gactgacctg cgccaatagt
     7561 taactgaatg cgattacggg catcggtcgt gagcaaatca gggatcggta acgttgggcg
     7621 ttccgccgca aagactgcac ggctccacag tggcaaggct gaagccacac ccagtgcgac
     7681 ggaatatttc aagaaatcac gacgttgcat agttatttcc ttattcttaa gcaggcgaaa
     7741 caaaatcaaa cgttgagcat agtccttccc cttacgggaa ggtcaagccg cagacacaat
     7801 ttaatcgaca ataatggtcg tcgcctcgct cgcagtgtgc taacgtttat cttctttaag
     7861 cccctggtag aagcaatgaa gacgtttttc agaacagtgt tattcggcag cctgatcgcc
     7921 gtttgcgcaa acagttacgc gctcagcgag tctgaagccg aagatatggc cgatttaacg
     7981 gcagtttttg tctttctgaa gaacgattgt ggttaccaga acttatctaa cgggcaaatt
     8041 cgtcgcgcac tggtcttttt cgcccagcaa aaccagtggg atctcagtaa ttacgacacc
     8101 ttcgacatga aagctctcgg tgaagacagc taccgcgatc tcagcggcat tggcattccc
     8161 gtcgctaaaa aatgcaaagc cctggcccgc gattccttaa gcctgcttgc ctacgtcaaa
     8221 taatccctga tacctttttg tagaaatatt gaccgtgcat ccgcggtcaa tgttagctat
     8281 tatgttgcgc ccttttttta cgggtgttaa caaaggaggt atcaacccat ggccgaaaaa
     8341 aaacagtggc atgaaacgct acacgaccag tttgggcagt actttgcggt agataacgtt
     8401 ctgtatcatg aaaagaccga tcaccaggat ctgatcattt ttgagaacgc tgcatttggt
     8461 cgcgtaatgg cgctggatgg cgtagtacaa accaccgagc gcgacgagtt tatctatcat
     8521 gagatgatga cccatgttcc gctactggcc cacggtcacg cgaaacatgt gctgattatc
     8581 ggcggcggcg acggtgccat gctgcgtgaa gtaacccgac ataaaaacgt tgagtcaatc
     8641 acgatggtgg aaatcgatgc gggtgtcgtg tcgttctgcc gtcagtatct gcccaaccat
     8701 aacgccggta gctacgacga tccgcgcttt caactagtga tcgacgatgg cgtcaatttc
     8761 gttaatcaaa ccagccagac ctttgatgtc attatctccg actgcaccga tcctatcggt
     8821 cccggcgaaa gcctgttcac ctcagcattt tatgaaggct gcaaacgttg cctgaatcct
     8881 ggcggtatct tcgtcgcaca aaacggcgtc tgctttttac agcaggaaga agccatcgac
     8941 agccatcgca aactcagcca ttacttcagc gacgttggct tttatcaggc ggcgatcccg
     9001 acctattacg gcggtatcat gacttttgca tgggcgacag ataacgacgc cttacgccat
     9061 ctctcaaccg aaattattca ggcgcgtttt ctcgcttctg gcctgaaatg ccgttattac
     9121 aatccggcaa tccatacggc agcttttgcc ttacctcagt atctgcaaga cgcactggct
     9181 tcacagccgt cctaaggaga agataagaaa ttgaaaaaac tgaaactgca tggctttaat
     9241 aatctgacca aaagtctgag tttttgtatt tacgatatct gctacgccaa aactgccgaa
     9301 gagcgcgacg gttatattgc ttatatcgat gaactctata atgccaaccg tctgaccgaa
     9361 atcctgtcag aaacctgttc cattatcggg gccaatattc ttaacatcgc ccgccaggat
     9421 tacgaaccac agggtgccag cgtcactatt ctggtgagtg aagaaccggt tgacccgaaa
     9481 ctcatcgaca aaacagaaca ccccggccca ctgccagaaa cggtggttgc tcatctcgat
     9541 aaaagtcata tttgcgtaca tacctacccg gaaagtcatc ctgaaggcgg tttatgtacc
     9601 ttccgcgccg atattgaagt ctctacctgc ggcgtgattt ctccgctgaa ggcgctgaat
     9661 tacctgatcc accagcttga gtccgatatc gtaaccattg attatcgcgt gcgcggtttt
     9721 actcgcgaca ttaacggtat gaagcacttt atcgaccatg agattaattc gattcagaac
     9781 tttatgtctg acgatatgaa ggcgctgtat gacatggtgg atgtaaacgt ctatcaggaa
     9841 aatatcttcc ataccaagat gttgcttaaa gagttcgacc ttaagcacta catgttccac
     9901 accaaaccgg aagatttaac cgacagcgag cgccaggaaa ttaccgctgc gctgtggaaa
     9961 gaaatgcgcg agatttatta cgggcgcaat atgccagctg tttaacggct ctggcggagc
    10021 tcccaggctc cgccagattt atttacttct gctgcacgaa attgcggtaa gccgccacga
    10081 cctgcagaaa atcctcaacg ccgcatagcg acaggctttc ttcgtcgtag tagttcatcc
    10141 cctcttccat ttcatcgcca gcgaattcca gttgattggc gcgaaccatc acctcttcag
    10201 cgtccatcca cagggtgtat tcatgccctg cccgttgcca ggaccgttcg ctacctttca
    10261 gtgcgtgcgc tgcttgttcc acttcatcaa gcaaggccag gttttctttc acctcttcat
    10321 taaaccagtg cccgaccact tcatgcccca tggacatacg cacctttacc actccggtaa
    10381 tatcgcgcag aaattcgtaa tccatagtgt gttcctctgc tcccggcaat gcgccgaaat
    10441 cgcatcgcgc cattgatgta attatcgcag ccttaccgga gaaaaacagc ggaacggcga
    10501 tagtttagaa ataaaaaatg cccgggaatc tcattcaccg ggcattgtgt cgtttatgcg
    10561 cagcgcgtgc gcttacttat tacaccgcag tctggaaaat caccccatcg gctttttcgg
    10621 tgtactgaga aagctggttg aagttcagat aacgataagt atcaacggca gttttatcta
    10681 cttgcgcaac gtaggtctgg tactcttccg gcgtcggcag tttgccaatc agcgccgcaa
    10741 cagccgccag ttctgcagaa gccaggaaga cattcgcgcc agtacccaaa cggttcggga
    10801 agttacgggt agaggtggaa accaccgtcg caccgtctgc tacacgcgcc tggttaccca
    10861 tacacaggga acagcccggg atctcgatac gagccccgct cttaccgaag acgctgtagt
    10921 agccttcttc cgtcagttgc gcggcgtcca tacgggttgg cggtgccacc cacaggcggg
    10981 tcggcaactg acctttgtgc gcatccagca gtttaccagc ggcacggaag tgaccgatgt
    11041 tagtcatgca agaaccgata aatacttcgt cgatcttctc gccctgtacc gcagacagcg
    11101 gacgtgcgtc atccggatcg ttcggagcac acaggattgg ctctttaatc tccgccagat
    11161 cgatgtcgat cactgccgcg tattccgcat ctgcatcggc ttccagcagc tcaggattcg
    11221 ccagccattt ttccatgccc tgaatacggc gttccagggt acgacgatcg ccgtaacctt
    11281 ccgcgatcat ccacttcagc aggacgatgt tggagctcag gtattcgatg atcggttctt
    11341 tgttcagctt gatagtacaa ccagcggcag aacgctcggc ggacgcatcg gttagctcaa
    11401 aggcctgctc aactttcaga tccggcagac cttcaatttc cagaatgcga ccagagaaga
    11461 tgtttttctt gcctttcttc tcaacggtca gcagaccttg tttgatcgca tacagcggaa
    11521 tggcgtgtac cagatcgcgc agggtgatgc ccggctgcat tttgcctttg aagcgcacca
    11581 gaacggattc cggcatatcc agcggcatta cgccagttgc ggcagcaaac gccaccagac
    11641 cagaacccgc cgggaaagag ataccgatcg ggaaacgggt atgggagtca ccaccggtac
    11701 cgacggtatc cggcagcagc atacggttca gccaggagtg aatgacgccg tcacccggac
    11761 gcagcgacac accgccacgg ttcataatga agtccggcag cgtgtggtgc gtgttcacgt
    11821 caactggctt cggatacgcc gcggtgtggc agaaagactg catcaccagg tcagccgaga
    11881 agcccaggca cgccaggtct ttcagttcat cacgggtcat cgggccggtg gtgtcctgag
    11941 agcctacaga agtcattttc ggttcgcagt acgcgcccgg acgaatgcct ttcacgccac
    12001 aggcacggcc taccattttc tgcgccagcg agaagccgcg atcgctctca gcgacatctt
    12061 tcgcctgacg gaacacatca ctgtgcggca gaccaagtgc ttcacgcgct ttggtggtca
    12121 ggccacgccc gataatcagc ggaatacggc cgccagcacg cacttcatca atcagcacgt
    12181 cggttttcag ttcgaaggtc gccagcagtt cgcctgtttc gtggttacgc acttcacctt
    12241 tgtacgggta aacgtcaatc acgtcgccca tgttcaggtt agagacgttg acttcgattg
    12301 gcagtgcacc cgcgtcttcc atcgtgttaa agaagatggg tgcaatttta ccgccgaggc
    12361 acaaaccacc gccgcgtttg ttcggcacat gtggaatatc atcgcccata aaccacagaa
    12421 cggagttcgt ggcggattta cgcgatgaac ccgtacccac aacgtcaccg acgtacgcca
    12481 gcgggaaacc tttctgttgc agagcttcaa tctgtttgat cgggccaaca acacctggct
    12541 ggtctggctc aataccttca cgggcgtttt tcagcatcgc cagcgcgtgc agtgggatat
    12601 ccgggcgtga ccacgcatcc ggtgccggag aaaggtcatc ggtgttggtt tcgccagtga
    12661 ctttaaagac ggtaacggtc agtttttcag ccagcgccgg gcgattcagg aaccattcgg
    12721 catccgccca ggactgcatt acctgcttcg catattcgtt gcctgctttc gctttctctt
    12781 ctacgtcata gaagttatcg aacatcagca gcgtgtgaga aagtgctttg gcagcaatag
    12841 gtgccagttt ggcatcatcc agcgcgtcga tcagcggatg aatgttgtaa ccaccctgca
    12901 tggtgcccag cagttcgata gctttttccg gagtcagcag aggggattta gcttcgcctt
    12961 tagcgatagc agccaggaag cctgctttta cataggcggc ttcatcgacg cctgggggaa
    13021 cacggttggt taacagatct aacaggaatt cttcttcgcc cgcgggcggg tttttcagca
    13081 gctctacaag cgcggccatt tggtttgcat ccaggggttt gggcgcaatc ccctcagcgg
    13141 cacgctcagc tacgtgctta cggtattctt ctagcacgac ggttctcctc gctctcattg
    13201 tcatagtgcg gcaggcgatt ctcttcacgc tcctgtgaga cagcagtttg tagggtaaat
    13261 gcccagtacc gcaacgggca gaatagcagg attttgacga cgtgttaatc tgtttacaaa
    13321 aaagcaacat aaaaaagtgg ctgaatcgtt aaggatggtc tagagattgt ttccctccgt
    13381 aaaaacttcg ttaattcgca tggtgataat cacctttctc aacgaatccc aagattagtc
    13441 aaaatttaaa ctaccgcctc tttatactcg gattcacagc acctgcgggt ggcagttcgc
    13501 cgaccattgc gatttccttg agatccgaat tatgaaaatg actttgccgt ttaaacccca
    13561 tgtgctggcg ctaatttgca gtgccgggct ttgtgctgcc tctgccgggc tatatataaa
    13621 aagccgcaca gtggaagcgc ctgtagaacc gcaatcgaca caactggctg tgtctgacgc
    13681 tgccgctgtt acgcttcctg caacggtttc cgcacctccc gtaatacccg cagtcgtcaa
    13741 atccgcattc agcactgcac aaatagatca atgggtcgca cccgtcgcgc tgtatcccga
    13801 cgccctactt tcgcaggtgc tgatggcatc aacctatccg acaaacgttg ctcaagcagt
    13861 gcaatggtcg cacgataatc cacttaaaca aggcgatgct gctattcagg cggtatctga
    13921 ccagccgtgg gacgccagcg ttaaatcact ggtggccttt ccacaattga tggcattgat
    13981 gggcgaaaac ccgcaatggg tgcaaaacct gggcgatgct tttctggcgc agccgcagga
    14041 cgtgatggac tcggtacagc gattgcggca actggcgcaa caaaccggct cgctgaagtc
    14101 atcaaccgaa cagaaagtta ttaccacaac gaagaaagct gtaccggtaa aacagacagt
    14161 cacggcaccc gtcataccat ccaataccgt tttaactgcc aaccccgtca ttacagagcc
    14221 ggcaacaacc gtcatttcca ttgagcccgc caatcctgat gtggtctata ttcccaacta
    14281 caacccaacc gtggtttacg ggaactgggc caatactgcg tatccgccgg tttatctgcc
    14341 accaccagcc ggagaaccgt ttgttgacag ctttgtgcgc ggattcggct atagcatggg
    14401 cgttgctacc acgtacgcac tattcagcag catcgactga gatgacgacg atcatgacca
    14461 tcatcatcat gacgatgatg attatcatca ccacgatggc ggtcatcgtg acagtaatgg
    14521 ctggcaacac aacggcgaca acatcaatat cgacgtcaac aatttcaacc gtatcaccgg
    14581 tgagcatctt actgataaga atatggcatg gcggcacaat ccaaactacc gtaatggtgt
    14641 gccctatcat gatcaggata tggcaaagcg gtttcatcaa accgatgtca acggcggaat
    14701 gagtgccacg cagctacctg ctccaacacg cgacagccag cgtcaggcgg cagcaagtca
    14761 gtttcagcaa cgaacacacg ccgcccccgt cattacacga gatacccaac gtcaggcagc
    14821 ggcacagcgg tttaatgaag ctgaacacta tgggagctat gacgacttcc gcgacttcag
    14881 ccgtcgccaa ccactgaccc agcagcaaaa ggacgccgct cgtcagcgtt atcagtcggc
    14941 ctctcctgag cagcgccagg cagtccgcga gaaaatgcag actaacccgc agatccagca
    15001 gcgaagggag gcggcgcgcc agcgtattca gtccgcctcg cctgagcagc gccaggcggt
    15061 tcgcgagaaa atgcagacta acccgcagaa ccagcagcga agagaggcag cgcgtgagcg
    15121 tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac agcagcgccc
    15181 actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat cacctgaaca
    15241 acgtcaggtt tttcgggaga aagttcagga gagccgccca caacgtctaa acgacagtaa
    15301 ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc tctctgaacg
    15361 tggagcaaga agactggaaa ggtaaattgc agacgtaaaa aaagcggcgt ggttagccgc
    15421 ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc tttcgggttc
    15481 ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga ctcgtgcaga
    15541 gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat ttcgattgcc
    15601 aggccgattt cacccagcag ctcgccgccg ttggtaccaa caatcgcacc accgatcaca
    15661 cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc agaagcgata
    15721 gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc tttctctttc
    15781 gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc gatggacggg
    15841 ataactttcg gatcgaagta gtgtttctta ccggcgataa cttcagcggc aacgtgacct
    15901 tcgtgaacac ctttgtgtgc cagcatcggc tgaccgacga tatcgccgat agcaaagatg
    15961 tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc gtcaacttcc
    16021 acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat cgctaccagc
    16081 acggcgtcgt aacgctgcgg ttcagctggt gcttttttgc cttccatcgt cacataaata
    16141 ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt gaatttcttg
    16201 ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat aacctggtcg
    16261 aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt aaacggtgcc catttccaga
    16321 ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag ttccagcgcg
    16381 tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg gatcgggcga
    16441 gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt ctcaccttca
    16501 acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt gactttgcgg
    16561 cctttcgcca taccagccag accaccggtc agctgattga tcactttctc tttccaggta
    16621 cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc agccagcgct
    16681 ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat acagccgacg
    16741 ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc cagacctaaa
    16801 tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac cacgacctga
    16861 gttttgattt cagtactcat catgacctct atatatttat ctccggcggt catacccgtc
    16921 gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt acttatgtaa
    16981 gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata gggtatgtga
    17041 agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa aattgttaac
    17101 aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc aatacattca
    17161 tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat caccagacgg
    17221 cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc agcaccgtcg
    17281 atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac gaactcttta
    17341 ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac ttccggcgcg
    17401 ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat ggtgaagcaa
    17461 ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga aatagtcatc
    17521 agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac cggaacaacc
    17581 agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag ggtcagacgc
    17641 tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc tgcagcaacg
    17701 gctttcatga tgaagacaac cggggtgatc ttcacatcca gcttacgttt cgccgcttct
    17761 tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc gaagtgggta
    17821 acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt ctggatacgg
    17881 cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg ccacggcagc
    17941 atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt gatagcttct
    18001 ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacggccagt gcccttcact
    18061 ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggggt cgcgtgaacg
    18121 taagcgtcgt tttcagcaaa ttcagatttg ccttccgctt tcgcagccgg agcttcagct
    18181 tttgctgccg gagccggcgc tgccgcttcc tgtttcgcag gagctgccgc aggcgctgcg
    18241 ccttcaactt cgaagatcat aatcagcgaa ccagttttca ctttatcgcc aacgttgact
    18301 ttcagttcct tcacgacgcc tgcaaacgga gccggaactt ccatagaagc tttatcgcct
    18361 tctacggtaa tcagtgactg ttcagcggca actttgtcgc ccactttcac catcacttcg
    18421 gtcacttcaa cttcgtcacc gccgatatcc ggaacgttaa cttctttcac gccagctgct
    18481 ggtgcagggg tcgctgccgg agtcgcttcc tgtttagccg ccggagctgc cgcgcctgct
    18541 tcacccgcga cttcgaagac cataatcagc gagccggtag acactttgtc acccacgttc
    18601 actttgatct ctttcacggt acctgcaaac ggagccggaa cttccataga agccttgtcg
    18661 ccttctacgg tgatcagcga ctgttcagct tcaactttat cgccaacttt caccaggatt
    18721 tcggtcactt caacttcgtc gctgccgata tccggaacgt taacgtcttt tgccgccgca
    18781 gccgcgggtg ctgctgccgg agctgcttct ttcttctctt ctgcctgagc aggtgcagcg
    18841 tctgctgcac cgtcggcgga atcgaaaatc ataatcagtg cgccggtctg ggttttatcg
    18901 ccaacagaga ctttgatctc tttaacgata cccgcctgcg gagatggaac ttccatagag
    18961 gctttgtcgc cttctacggt gatcagcgac tgttcggctt caactttgtc gcccactttg
    19021 accaggatct cggtgatttc aacttcatca gccccgatgt ccggtacttt gatttcgata
    19081 gccattattc ttttacctct tacgccagac gcgggttaac tttatctgca tcgatgttga
    19141 atttggcgat tgcgtcagca accactttct tatcgatttc gccacgttta gccagttcgc
    19201 ccagcgccgc aaccacgaca taagaagcat caacttcgaa gtggtgacgc aggttctcac
    19261 ggctgtcgga acgaccgaag ccatcagtac ccagtacgcg atagtcgtca gccggtacgt
    19321 aagtacggac ctgctcagcg aacagtttca tatagtcggt agatgccact gccggcgcgt
    19381 cgttcatcac ctgagcgata tacggaacgc gcggagtttc cagcggatgc agcatgttcc
    19441 agcgttcaca atcctgacca tcacgcgcca gctcggtgaa ggaggtcacg ctataaacgt
    19501 cagaacctac gccgtaatct ttcgccagga tctctgccgc ttcacggacg tgacgcagga
    19561 tggaaccgga gcccagcagc tgaactttac ctttgctacc ttcaatggtt tcgagtttgt
    19621 agataccttt acggatacct tcctcagcac cttccggcat tgccggcatg tggtagtttt
    19681 cgttcagcgt agtgatgtag tagtaaacgt tctcttgttt ttcaccgtac atacgctcca
    19741 gaccgtcatg cataatgaca gcaacttcgt aagcgtaagc cgggtcgtaa gagatacagt
    19801 tcgggatagt cagcgactga atgtggctgt gaccatcttc gtgctgcaga ccttcaccgt
    19861 tcagggtggt acgaccggaa gtaccgccga tcaggaagcc acgcgcttgc tggtctccag
    19921 ccgcccagca cagatcgccg atacgctgga agccgaacat cgagtaatag atgtagaatg
    19981 ggatcatcgg cagattgttc gtgctgtaag aggtcgccgc tgccagccag gaacaccctg
    20041 cgcccagctc gttgatccct tcctgcagaa tctgaccttt ctcgtcttct ttatagtaag
    20101 caacctgctc gcggtcctgc ggggtgtact gctgaccgtt cgggctgtaa ataccaatct
    20161 gacggaacag accttccata ccgaaagtac gcgcttcgtc ggcgatgatc ggtaccagac
    20221 gatctttgat cgacttgttc ttcagcatca cgttcagagc acgaacgaaa gcgatagtgg
    20281 tagagagctc tttgctctgc tcttccagca gcgcgccgaa gtcttgcagg ctcggcagct
    20341 caagcttctc ggtgaagttc ggctgacggc ttggcagata accgtgcagt ttctgacgct
    20401 gagcgtgcag ataggtatgc tcttcagaac cttccgggaa ggtgatgtac ggcagttttt
    20461 cgatatctgc atcagacacc ggcacattga aacggtcgcg gatgtgacgc acgccgtcca
    20521 tgttcatttt cttaacctgg tgcgcgatgt ttttaccttc agccgcgtcg cccatgccgt
    20581 aacctttaat ggtatgagca aggattactg tcgctttgcc tttggtttcc tgcgctttct
    20641 tgaatgcagc gtagattttc ttcggatcgt gaccaccacg gttcagtgcc cagatctgct
    20701 cgtcagtcca gtctgcaacc agtgctgcgg tttcaggata tttaccgaag aagtgttcac
    20761 gaacgtacgc accatctttc gatttgaagg tctggtagtc gccgtcaacg gtttcgttca
    20821 tcagctggat cagtttaccg ctggtatctt tacgcagcag ttcatcccaa cggctacccc
    20881 acatcacttt gatcacgttc cagccagcac cttcgaagat gccttccagt tcgttgatga
    20941 tcttgccgtt accggtgacc gggccgtcaa gacgctgcag gttacagttg ataacgaaaa
    21001 ccaggttatc cagtttttca cgggtagcga tggtgatcgc acctttggat tccggttcgt
    21061 ccatttcacc gtcgccgagg aacgcgtaaa cggtctgttt agaggtatct ttcaggccac
    21121 ggtgttccag atatttcagg aatttagcct ggtaaatagc accaatcgga cccagaccca
    21181 tagatacggt cgggaactgc cagaattccg gcatcagttt cgggtgcgga taggaagaga
    21241 ggccattgcc gtgaacttcc tgacggaagt tatccagctg ctcctgagtc agacgacctt
    21301 ccaggaaagc acgggcgtaa acgcccgggg agatgtggcc ctggaagtaa accaggtcgc
    21361 cgccatcctg ctcgttgcgt gcacggaaga agtggttaaa gcacacatca taaatggttg
    21421 cggaagactg gaaggacgcc atgtggccgc ccagttcgag gtcttttttc gacgcacgca
    21481 gcaccgtcat gatggcgttc cagcggatag ctgaacgaat acggcgttcc agttccagat
    21541 tacccggata ctccggttgt tcttcaacgg ggatggtgtt gatgtagttg ctgatacctg
    21601 tgcctgcggc tacgtttaca ccgcctttgc gggcttcagc aagcagttgg tcgatcagat
    21661 actgagcacg ctcaacacct tcttcacgga tgaccgattc gatcgcctgg agccagtcgc
    21721 gagtttcgat cggatccacg tcatttggga aacgttctga catgggttat tccttatcta
    21781 tctaataacg ttgagttttc tggaacctgt cccattgaac tctcgccaga aagctcaata
    21841 agacaggttc tacgtttagt taccgcgctt ttatatgcgc ttgatttaca acatcttctg
    21901 gataattttt accagaaaaa tcactaattc tttcgttgct ccagacgacg cagagaacgc
    21961 tcacggcggc tctcttcacg gcttctgtcg agcaaaattt cttcgataaa ggccagatgg
    22021 cgatgcgatg cttcgcgcgc ttcttccggc ttaccgacca taatcgcttc aaatatgcgg
    22081 gtgcggtgac tactcaccag cggcagcatc tcgcgacgcg aatagagcaa ttcgaagttc
    22141 tggcggacat tctgggccaa catcggctcc atacacctta gcagatgaag cagaaccaca
    22201 ttgtgggccg cttcggtgac ggcaatctga tactggagta cggcgtttga ttccgcgtcc
    22261 agatcgccag actgctgcgc cagctctatg gcgtggtgga gttcacggat gcgttccttg
    22321 tcttcatcgg tactacgcag cgcggcgtaa taagcggcga taccttccag ggcgtgtcgt
    22381 gtttcgagca agtcatactg tgattcagga tggtcggaga gcagctccac cagcggatcg
    22441 ctgaagcttt gccataggct gctctggaca aaagtgccgc caccctggcg acgaagcaac
    22501 aagcccttcg cttcgagacg ttgaatcgcc tcacgcaagg agggacggga gacgtcgaac
    22561 tgttttgcca gttcgcgttc cggtgggagt ttttcgcccg ggcggagagt gccttcgagg
    22621 atcaaaaact ccagttgctg ctcaatcaca tcggagagtt ttggttggcg gattttgctg
    22681 taggccatga gttcctgtct taagccactt gccgaagtca attggtctta ccaatttcat
    22741 gtctgtgacg ctaaagtaac aaagtattca ccttatgtcc atacaggttt tgattgaaat
    22801 catgaaactg tgcacatttt aacaacttga catatataac gtttcaaagt tgtaactatg
    22861 cacaaatgta gactttacgt aggaaaggga ttttttaacg attattaaac tataaaaatc
    22921 cgaattgaac cgattcactt accaattttt tgatttttaa gtcaataaaa acgaatttaa
    22981 attcactctg catattgaga ggggttgagg ctgagcttta caaacggttt ctttttaagc
    23041 aactcatctt caaccatgca taaagcgggt gcattcgctg ccgcatacca ttattcttga
    23101 tctgacggaa gtctttttgt aacaattcaa acctccttga tgtaaacaaa tcaatacaac
    23161 aaacggaatt gcaaacttac acacgcatca ctgcgtagat caaaaaaaca atcaccgcac
    23221 gaggtttcat gatggaaggt caacagcacg gcgagcagct aaagcgcggc cttaaaaacc
    23281 gccatattca gcttatcgcg ctgggtggcg cgatagggac agggctattc ctaggtagcg
    23341 cctccgtaat acagtccgca gggccaggga ttatcctggg ttacgccatt gctggtttta
    23401 tcgcctttct gatcatgcgt cagctgggtg aaatggtggt cgaagaacct gtcgcaggct
    23461 cctttagcca ctttgcttat aaatactggg gcagttttgc cggtttcgcc tctggctgga
    23521 actactgggt actgtacgtt ttagttgcca tggcagagct gactgccgtg ggtaaataca
    23581 ttcagttctg gtatccggaa atcccaacct gggtttctgc cgccgtattc tttgtggtga
    23641 ttaacgccat caacctgacc aacgttaaag tgtttggcga gatggagttc tggtttgcca
    23701 ttatcaaagt tattgcggta gtagcgatga tcatcttcgg cggctggctg ctgttcagtg
    23761 gcaacggcgg cccgcaggcg agcgttagca acctgtggga tcaaggtggt ttcctgccgc
    23821 acggcttcac cggactggtg atgatgatgg cgattatcat gttctcgttc ggtggtctgg
    23881 aactggtggg gatcaccgca gcagaagctg ataacccgga gcaaagtatc ccgaaagcaa
    23941 ctaaccaggt tatctaccgc atcctgattt tctatattgg ttcgttagcc gttctgctct
    24001 cactgatgcc gtggacccgc gttaccgccg ataccagtcc gtttgtgctg atcttccacg
    24061 agttaggcga tacctttgtg gcgaatgcgc tgaacatcgt ggtactgact gcggcgctct
    24121 ccgtgtacaa cagctgcgta tattgcaaca gccgtatgct gtttggtctg gcacaacagg
    24181 ggaacgcgcc aaaagcgctg gcgtctgtcg ataaacgcgg cgtaccggtt aacaccattc
    24241 tggtgtctgc gctggttaca gcattgtgcg tattgattaa ctatcttgct ccggaatccg
    24301 catttggcct gttaatggca ctggtggtat ccgcactggt gatcaactgg gcgatgatca
    24361 gtctggcgca tatgaagttc cgtcgcgcca agcaggaaca aggcgtggta actcgcttcc
    24421 ctgctctgct ttatccgctg ggtaactgga tctgcctgct gtttatggcg gcggtactgg
    24481 tgattatgct gatgacccca ggaatggcga tttcggtata cctgatcccg gtatggctga
    24541 tcgtgttagg tatcggctat ctgtttaaag agaaaaccgc caaagccgta aaagcgcatt
    24601 aatctctcta cgccctcacc cgtacagggt gagggcaata atctctacac caacgccccg
    24661 taaatcgtca gtagcgcaat caccaccacg accacgaacg aggttttctt cgccattgaa
    24721 accgctgcct tcggcgtctc caccttatcg acatgcggtt cacgcgccag agagaactgc
    24781 gccagacgcg ttaatacctg atattgcgaa gtatggaaat cacccagcga agcaaaccag
    24841 gccggtaacg ctttctcacc atgaccgatc aaggcatata ccacacccgc aagacgaacc
    24901 ggcacccaat ccagtacatg aagcactgca tcaatgccgg actgtaaacg atgatgcggc
    24961 gtctggtatc gcgccagcca gtattgccat gcacgcaaga acgcataccc catcagcgta
    25021 acgggtcccc aggttccccc cacaatcagc cagaacagcg gtgcaagata aaaacgaaag
    25081 ttaatccaca gcaatgcatt ttgcagctca cgcaaatact cacgttcgtc gcagcctgcc
    25141 gggacgccgt gaatcatggt gagttcgcca gccatcgtgg cacgggcatg gctatcatta
    25201 cgtgaagcag ctgtcagata agcatgataa tgaagacgaa ctttacctgc gccaatacac
    25261 agcaaaccaa tcagcagcca caccagcagc gtgggaacgt tgaacaatac tccctgcaat
    25321 gcgcgtaaca gtaaaaaagt cacacccatc gcaataatgg tcatgcctaa cgtgcgcccg
    25381 agagaaaaat gtttcacccg ccgaaagaac gcttcaagac gatgatcaag ctgccagtgc
    25441 tcgcccaact taaacaggcg ctcgaaaatt aacaccagta aggttgtaaa tagcgtcatg
    25501 ttgtctcctt gctgaccagc gcacgaaacc gtgcccaatc aaatgcagga ccgggatcgg
    25561 ttttccgatc cggcgcaata tcacaatggc ccgtcatgtt attagcgata tcgggatagc
    25621 gatctatcag tgcccgcgta accgccgcaa gctgttgata ctgcgcatcg gtatacgcca
    25681 gagtatcggt gccttcaagc tcaatcccaa tagaaaaatc attgcagcgt tcacgcccct
    25741 gatactgaga gactcccgca tgccaggcac gtttatcgaa aggaacatac tggactattt
    25801 caccatcacg gcgaatcaaa cagtgagcgg agacgcgcaa atgggcgatc tcagcaaaga
    25861 aaggatgtgc ctgcggatca atagttccag tgaataatgc gtcgatccac ggaccgccaa
    25921 actcgcctgg cggcaggcta atattgtgca ccaccagcag ggtgggtgtt tcgtcatcca
    25981 ggcggcaatc gtaatgtggt gagggaacgc ggcgcgcgcc aaccagccac ccctgttcta
    26041 acaacatgca ggatctcctt atatgtggtg ctaatacccg gttcagagta gcatgtttct
    26101 accttatgat tcgttagcta tctggagttt taacatgccg cctcgccgct acaaccctga
    26161 cacccgacgt gacgagctgc tggaacgcat taatctcgat atccccggcg cggtggccca
    26221 ggcgctgcgg gaagatttag gcggaacagt cgatgccaac aatgacatta cggcaaaact
    26281 tttaccggaa aattctcgct ctcatgccac ggtgatcacc cgcgagaatg gcgtattttg
    26341 tggcaaacgc tgggttgaag aggtgtttat tcaactggca ggcgacgatg tcaccataat
    26401 ctggcatgtg gatgacggcg atgtcatcaa cgccaatcaa cccttgttcg aacttgaagg
    26461 cccatcccgc gtgctgttaa cgggcgaacg cactgcgctt aattttgtgc aaaccctttc
    26521 aggagttgcc agtaaggtac gccactatgt cgaattactg gaaggcacca acacgcagtt
    26581 gttggatacg cgcaaaacct tacctggcct gcgttcagct ctgaaatacg cggtactttg
    26641 cggcggcgga gcgaatcacc gtctggggct ttctgatgcc ttcctgatca aagaaaacca
    26701 tattattgcc tccggctcag tgcgccaggc ggtcgaaaaa gcgtcctggc tgcatccgga
    26761 tgcgccagta gaagtcgaag tagagaatct ggaagaactt gatgaggccc tgaaagccgg
    26821 agccgatatc atcatgctgg ataacttcga aacagaacag atgcgcgaag ccgtcaaacg
    26881 caccaacggc aaggcgctac tggaagtgtc tggcaacgtc actgacaaaa cactgcgcga
    26941 atttgccgaa acgggcgtgg actttatctc cgtcggtgcg ctaactaaac acgtacaagc
    27001 actcgacctt tcaatgcgtt ttcgctaatt gctgacagat ttatccgctc catcgcgggc
    27061 ggataatctc cccttcccca actttcttcg taacgcctcg caaatttctt gtctttcgct
    27121 gcaacgaaat aaaaaacctc ggaatctgct ttcccgtttc gttttttgcc cctcgcctgt
    27181 gctgatggcg tttgccaaag tagcaccaac caaatcaagg agcgaaacag atggacaagc
    27241 aacgcggttt tacacttatc gaactgatgg tggttattgg catcattgcc attttaagct
    27301 ccattggtat tcccgcttat caaaactacc tgcgcaaagc cgcactcacc gacatgctac
    27361 aaacctttgt gccttaccgt accgccgtag agttgtgcgc gctggaacat ggtggattag
    27421 atacctgcga cggtggcagc aatggcattc cctcgcctac caccacccgc tatgtttcag
    27481 ccatgagtgt ggcaaagggc gtggtgtcgc tgaccgggca agaaagtctc aatgggctaa
    27541 gcgtcgtcat gacgccaggt tgggataacg caaacggcgt caccggctgg gcgcgcaact
    27601 gcaatattca aagtgacagc gcattgcagc aagcctgcga agatgtcttc cgctttgatg
    27661 acgccaacta aggagcggca atgaatattc cacagctcac ggccctgtgc ctgcgttatc
    27721 agggagtctt gctggatgcc agcgaagaag tggttcatgt tgcggtggtc gatgccccct
    27781 cacatgagtt gctggacgca ttgcatttcg ctaccaccaa acgtattgag atcacctgct
    27841 ggacgcgcca acaaatggaa ggtcacgcca gtcgcacaca acagacatta cccgtagctg
    27901 ttcaggagaa gcatcagccc aaagcagagt tgctgactcg aacgttacaa tctgcgctgg
    27961 aacaacgcgc gtctgatatt catatcgaac cagcggacaa tgcctaccgc atccgcttgc
    28021 gtatcgacgg cgtattgcat cctttaccgg atgtttcacc ggatgccgga gtcgcattaa
    28081 cagccagatt aaaagtgctg ggaaacctgg atattgcgga acatcgcctg ccgcaggacg
    28141 ggcaattcac tgtcgaactg gcaggaaaca ccgtctcatt tcgtattgcg accttaccat
    28201 gtcggggtgg tgaaaaggtg gtattaaggt tgttacagca ggtgagccag gcactggatg
    28261 ttaacacgct gggaatgcag ccgttacaac tggcggactt tgctcatgcc ttgcaacaac
    28321 cacagggact ggtgctggta actggcccta caggcagtgg caaaacggtc acctggtgtt
    28381 gtccacgcta cacaccaatt ccaccactga aacgctggta cgtttacagc aaatgggggt
    28441 cgcccgctgg atgctatcat cggcgcttac gctggtaata gcccagcgtc tggtacgtaa
    28501 actttgccca cattgtcgcc gacagcaagg ggagcccatc catattccag acactgtatg
    28561 gccgtcgccg ctgccccact ggcaggcacc cggttgtgta cattgctacc acggttttta
    28621 tggtcgtacc gccttgtttg aagtcctgcc cattacacca gccatacgcc agcttatttc
    28681 cgctaatact gaagttgaat cgctggaaac gcacgcccga caggcgggta tgcgtacgct
    28741 ttttgaaaac ggctgcctgg ccgtggagca aggcttaacc acctttgaag agttaatccg
    28801 cgtactgggg atgccgcatg gcaagtaagc aactctggcg ctggcatggc ataaccggcg
    28861 acggcaatgc gcaagatggg atgctatggg cagagagccg tactttactg cttatggcac
    28921 tacagcaaca gatggttacc ccactaagcc tgaagcgaat cgccatcaat tctgcgcagt
    28981 ggcgaggaga taaaagcgcg gaagtcattc atcaactggc gacgctactt aaagccgggt
    29041 taacgctttc tgaagggctg gctctgctgg cggaacagca tcccagtaag caatggcaag
    29101 cgttgctgca atcgctggcg cacgatctcg aacagggcat tgctttttcc aatgccttat
    29161 taccctggtc agaggcattt ccgccgctct atcaggcgat gatccgcacg ggtgaactga
    29221 ccggtaagct ggatgaatgc tgctttgaac tggcgcgtca gcaaaaatcc cagcgtcagt
    29281 tgaccgacaa agtgaaatca gcgttgcgtt atcccatcat cattttagcg atggcaatca
    29341 tggtggttgt ggcaatgctg cattttgttc tgccggagtt tgccgctatc tataagacct
    29401 tcaacacccc actaccggca ctaacgcagg ggatcatgac gctggcagac tttagtggcg
    29461 aatggggctg gctgctggtg ttgttcggct ttctgctggc gatagccaat aagttgctga
    29521 tgcgccgccc gacctggctt atcgcgcggc agaaattgct gttacgcatc ccgattatgg
    29581 gttcactgat gaggggacaa aaactcacgc agatctttac gattctggcg ctgacacaaa
    29641 gtgcaggcat tactttttta cagggcgtag agagcgtcag agaaacaatg cgctgcccgt
    29701 actgggtgca acttctgaca caaatccagc acgatatcag taacggtcat cccatctggc
    29761 tggcgctaaa aaatgccggt gagtttagcc cgctctgttt gcaattagtg agaacaggag
    29821 aggcatccgg ctcgctggac ctcatgttag acaacctcgc ccatcatcat cgggataaca
    29881 caatggcgct ggcggataac ctcgcagcct tactggaacc ggcgttgctg atcataacgg
    29941 gaggaattat cggtacgctg gtggtggcaa tgtatctgcc aattttccat ttaggcgatg
    30001 cgatgagtgg gatgggataa cgctggcgtg ttgctccacg ccagcgttgg gagattacag
    30061 gttgttgaag atgcggtttt cttgttccag cacacgaata aacgtggtgc gcttggtcag
    30121 ctctttcagg cgtgaagccc caacgtatgt acaagctgaa cgcaggccgc ccaaaatatc
    30181 acgcgcggta ttttcaacct ggcctcgcag cggcagctta acggttttac cttctgctgc
    30241 gcggtattcc gcaacgccgc caacgtgacg tttcatcgca gactcggagc tcatgccgta
    30301 gaacagcata aatttctcgc cgttctcctc aacgatgcga ccgccgctct cttcgtggcc
    30361 cgccagcatg ccgccaagca tgacgaaatc ggcaccgccg ccaaaggctt tcgccacatc
    30421 gcccggcgtg gtacagccac cgtcgctgac gatcattccg cccagaccgt gcgccgcatc
    30481 ggcacattcg attaccgcag aaagctgcgg ataaccgacg cctgttttga cgcgagttgt
    30541 acaaacagaa cctgggccaa tgccaacttt aacgatatca gcaccggaga ggataagctc
    30601 ctcacacatt tcaccagtca ctacgttgcc agcacaaatg gttttggtcg gccacgcttc
    30661 acgcgctttc gcaacgaact gcacgaagtg ttcggaataa ccattcgcca catcaataca
    30721 aacgaagttt aatgccgggt tcaggtcgag aatctgttta gttttttcga aatccgcatc
    30781 agacgtgccg gtagaaacca tcacatgttt cagcacatca gcggaagaat tgttgataaa
    30841 cgcttgccac tcttcgacag aaaagtgttt atgcacagca gtcagaatat caaaagaagc
    30901 cagcgcagag gccatagaaa atgtgcctac ggtgtccata tttgcggcga taatcggcac
    30961 gccggaccag ctctgacctg aatgtttgaa ggtgaattga cgttccagtt caacatcgga
    31021 acggctttta agagtggagc gtttagggcg gatgagaacg tctttaaaac ctaacttcag
    31081 atcttcttca atacgcatgt gcggattcct ggggttaatg gctataaggg taagagcgca
    31141 actccagtga cgctatcata cgcactaatc aatgcggcgc aagactgcga aattgtcttt
    31201 ttttacgcta caatcccata aatttacctg gtaaatagtg agcgaacgtt tcatcaattt
    31261 cactttcgcg cgctcatttt taacgttttg ataatcatga tgaattcccg ggaatataaa
    31321 ttatggggta tatagttgcc ttaacgggag gcattggcag tggtaagagt accgttgcca
    31381 atgcgtttgc tgatctcgga attaacgtca ttgatgccga tattattgcg cgtcaggtgg
    31441 ttgaaccagg tgcacctgcg ctacatgcca ttgctgatca ctttggcgaa aacatgattg
    31501 ctgctgatgg aacattgcag cgccgggcct tgcgcgagcg gatcttcgcc aacccggaag
    31561 agaaaaactg gcttaacgac ctgctgcatc cgctgattca gcaagagacg caacaccaga
    31621 tccagcaagc aacttccccc tatgtactgt gggttgtgcc attgctggta gaaaactcac
    31681 tgtataaaaa agcgaatcga gtgctggtgg tggatgtcag cccagaaacg caacttaagc
    31741 gcaccatgca gcgcgatgat gtaactcgcg aacatgtcga acaaatcctt gctgctcagg
    31801 caacgcgcga agcccgcctt gccgtggcag atgacgtcat tgataataac ggcgcaccgg
    31861 atgctatcgc atcggatgtt gcccgcctgc acgcacacta tttgcagctt gcgtcgcagt
    31921 ttgtctcaca ggaaaaaccg taatgcagac ccaggtcctt tttgaacatc cactaaatga
    31981 aaaaatgcgt acatggctgc gcattgagtt tttgattcag caactcaccg ttaatttacc
    32041 catcgttgac cacgctggcg cgctgcattt cttccgtaat gtcagtgaat tactggatgt
    32101 tttcgagcgc ggcgaagtcc gcactgagct gttgaaagaa cttaaccggc agcaacgtaa
    32161 actccagacc tggattggcg tgcctggcgt ggaccagagc cgtattgaag cattaattca
    32221 gcagttaaaa gcggcggtga gcgtattaat ttccgcgccg cgtatcgggc aatttctgcg
    32281 tgaagatcgt ttgattgctc tggtgcgtca gcgactgagc atcccaggcg gctgttgcag
    32341 ctttgattta cctacattgc acatttggct gcatctaccc caggcgcagc gcgacagcca
    32401 ggtagaaacc tggattgcca gcctgaaccc gctcacccag gcacttacca tggtgctgga
    32461 tttaattcgc cagtcggccc ccttccgtaa acaaaccagc ctgaatggtt tttatcagga
    32521 taacggtggc gatgccgact tgctgcgcct gaatctgtcg ctcgattcac agctttatcc
    32581 gcaaatttcc ggacataaga gccgttttgc cattcgtttt atgccgctgg acactgaaaa
    32641 cggacaggta ccggaacgtc tggatttcga actggcctgt tgctaaggag taaaaatgtc
    32701 agaaactatt acggtgaatt gcccaacctg cgggaaaacg gtggtgtggg gtgaaatcag
    32761 cccgtttcgg ccattttgct ccaaacgttg tcagctgatc gacctcggag aatgggctgc
    32821 tgaagaaaaa cgaatcccca gcagtggcga tctttccgaa agcgatgact ggagcgaaga
    32881 gccaaagcag tgacatttgt ctgatgcctc acgtaggcct gataagacgc ggacagcgtc
    32941 gcatcaggca tcttgtgcaa atgtcggatg cggcgtgaac gccttatccg acctacagac
    33001 gcttaagctt cgcaattacc ggttcattgg ctggcggaaa atcatcggca ttaagaccga
    33061 ccagcgacat ccactcaccg ggttgccctt ctttacccca cggcacccct tcccaacttt
    33121 cgaccagcca gaaccacaga gttatatgcc tgtccgggaa ttcatattcc agtttttcaa
    33181 atagcgaaaa atgttgtggg gtaatcccga cctcttcctg aagttcacgc accaccgcct
    33241 gttccggcgt ttcgcccatt tcaattttac cgccgggaaa ctccagttta ttcgccatgt
    33301 gcgcatctgc tgcgcgacgt gttataaaga tttcattgtt ctcgttgcga ataataccta
    33361 ccgcaatttg cagctttttc attgtcttaa acctataaaa aaggcgcaga atcctgcgcc
    33421 ttttacttca actgttagct tttattgcag gcgaccatgg cactgcttgt attttttacc
    33481 agaaccgcac gggcaaggat cgttacgtcc tactttgcgt tcaccggttt gcgccgccag
    33541 tgcagctgcg gctgcagagt cgtcatcctg atggctaagc tgctgcattt gtgctaaacg
    33601 ctcggcttcc atacgacgct gttgttccag ctcctcaacc tcttcaggca tacgtacctg
    33661 aactttgctc agcgtactga taacttcata tttcaacgac tccagcatcg ctgcaaacat
    33721 ggagaacgat tcacgtttgt attcctgctt cggatctttc tgtgcatagc cacgcaggtg
    33781 gataccctga cgcagatagt ccatcgctgc caggtgctct ttccacaggg agtcaagcgt
    33841 ttgcagcatg acgcctttct cgaagtgacg catcatctca gcaccaacca cttcttcttt
    33901 acgctgatac acttcgatgg actgcgccag aatgcgctca cgcagcgtct cttcatgcag
    33961 ttctggttct ttatccagcc actcggtaat tggtaaatcg aggtcgaaat cgttcttcag
    34021 acgttcctgc agccccggaa tatcccacat ttcttccagc gactgtggcg gaatgtaggc
    34081 atcaatggtg gctttgaaca catcttcacg aatgctgttg atggtttcgc tcacatcgct
    34141 gacatccagc agttcgttac gctgggagta aatggcgcga cgctgatcgt tagccacgtc
    34201 atcatattcc agcagttgct tacgaatgtc gaagttacga ctttcaactt tacgctgggc
    34261 gttggcaatc gctttagtca cccacgggtg ttcaatggct tcgcctggct tcatacccag
    34321 tttacgcatc atgccggata ctcggtcgga agcaaaaata cgcatcagcg catcttccat
    34381 cgacaggtag aaacgggaag aaccagcatc cccctgacga ccagaacgac cgcgcaactg
    34441 gttatcgata cgacgggatt cgtgacgctc ggtaccgatg atatgcaggc cacctgcttc
    34501 cagtaccgca tcgtgacgta cctgccagtc ggctttaatt ttttcaattt gctctgcggt
    34561 cggattttcc agcgcggcaa cttctgcctg ccagctacct ccgagcacaa tatctgtacc
    34621 acgacccgcc atgttggtcg cgatagtcac cgcagccgga taacctgcct gagcaacaat
    34681 cgccgcttcg ttggcgtgga atttggcgtt caggacattg tgcttaatac cggctttggt
    34741 cagttcgttt gacaccagct ccgatttttc gatggagata gtacccacca gcaccggctg
    34801 gcctttcgca gtacgttctt tgatatcttc aatgatcgcc tgaatttttt ccgcttcagt
    34861 catgtagacc aggtccggca gatctttacg aatcattgga cggttggtcg gaacaacgac
    34921 ggtatccagt ttgtagatgg agctaaattc gaaagcttcg gtatcagcag taccggtcat
    34981 ccccgccagt ttttcataca gacggaagta gttctggaag gtgatcgaag ccagcgtttg
    35041 gttttcgttc tggatctgca caccttcttt cgcttccaca gcctggtgca gaccatcgga
    35101 ccagcgtcgg ccctgcatgg tacgaccggt gtgttcgtca acgatgataa cttcaccatc
    35161 tttaacgatg tagtcgacgt cacgggtaaa cagcgcatga gcgcgcagcg ccgccgttac
    35221 gtggtgcatc agcatgatgt tggccggaga gtacagagac tccccttcat ccatgatgcc
    35281 ctctttcacc agcagttctt caatcagcac cagaccacgt tcggtcaggt tcacctggcg
    35341 agatttttcg tccaccgaga agtggccttc gccctggaag gtttcggagt cttctttttc
    35401 ctgacggatc aggtgcggaa taattttatt cacgcgttta tacatttccg agctgtcttc
    35461 tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg atcaggatgg agtccacttc
    35521 gtccaccagc gcatagtgca gtttacgctg tacacgttct tcagggctga acgccatgtt
    35581 gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg taagtgatgt cagctgcgta
    35641 agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg ataccaacag tcaggccaag
    35701 gaattcaaac agcggacggt tgttttcggc gtcacgttgc gccaggtagt cgttgacggt
    35761 aactacgtgc acgcctttac cggttagtgc gttcaggtaa gcaggcagcg ttgcggtcag
    35821 ggttttacct tcaccggtac gcatttcggc gatgcagcgt tcgttaagaa ccataccgcc
    35881 gagtaactga acgtcgaagt gacgcatacc aaagacgcgc ttacttgcct cacgcaccac
    35941 ggcgaaagct tccgggatca gattttccag cacttcgcct ttttccagac gcgcacgaaa
    36001 ctctgcggtt ttccctttca gttcttcatc ggagagtttt tccatctccg gttccatggc
    36061 attgatgatg ttgaccactt tgcgcatccg gcgcagggtg cgatcgttac gactaccgaa
    36121 aactttagtt aacaatttga ttagcataat aaaatctcaa acgccccgcg ttacggagtt
    36181 aataaaatga agtaaaggtt tattgttgtt aggtgaggcg ttgagggcca gcacggatgc
    36241 cttgcgcctg gcttatccag acgggcgtgc tgaattttgc ttgtggggta aaatgcgcat
    36301 aatcaatgcg ataacccttt tcagacggcg tgccttcctg ggtcagcagc gcgctgagcg
    36361 tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga ttcttcagca acgggcagtg
    36421 tttgcggtgc cattgcgaaa gaaagatgac ggattaccgt acgaatagca tgttgatgcc
    36481 agtaatcaac ggaatagttc gaattcgggc ggcgcgtgtt cgcttccagc aaggccaatt
    36541 gaccaaagtt aactttggct gaaggctcgt ggttgcgggt tgtcgctttt gcgggcgcgt
    36601 ttggttcggc ggcgttgctg agcgcaggca aacctaaact cgccgcgacc atccctaata
    36661 agagatgcgg ccagaagtag cgtttaccaa actgtcgcca gcgcgtcagt attccactca
    36721 cgttattgcc atcccgatcg aattcaaacg tatttagcgc acaaatctta tcattaaagc
    36781 ctgaagtcca cagcacgaat gaagataagt tgtgcgtaaa agtgcttaag aaaacagcgt
    36841 tcgcaccgtt ttttatagct cttataaaat tcaaaaaaaa acgactggtt cacctggccg
    36901 gagagagtgc cagatttgcc agtcgaattt tatacgacag tataaatgtc gttatgccag
    36961 tacagctgaa ggcgctttga aggccaacgg cagttctgcg tcgtcctgga aggtcacata
    37021 ttcccaggct tcctgtttcg ccaggacagc ctgcagcagt ttgttattca gtgcatgacc
    37081 ggatttataa gcggtaaatg caccaataat attgtgacca cacatgaaca agtcaccgat
    37141 cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa cgcaggccgt cttcgttcag
    37201 tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg ccgcccaggc acaaaccacg
    37261 ggactgcaga tattcgatat cacgcatgaa accgaacgta cgcgcacggc tgatctggcg
    37321 cataaacgca tcagcggaga agttcatcgc atagcgctgg ttgctggaat caatagccgg
    37381 atggttaaaa tcgatggtga aatccagcga aaaaccattg tacggcttaa attcagccca
    37441 cttatcgcca tcttcgacac gaacagtctc tttgatgcga acaaattttt tggcgcagtt
    37501 caactcgtcg ataccggcgt caagcagcag gtatacaaac ggagcggcgc tgccgtccat
    37561 gatcgggatt tccggcgcgt taacttcgat aacaatgtta tcgatgccca ggcccgcgag
    37621 agcagcattg aggtgctcta cggttgaaat ccgtacatca tgctcgttga ccagacacgt
    37681 acagagcatg gtatcacgca cagatttggc atcggccggg aaatctaccg gtggattcaa
    37741 gtcggtgcga cgatagatga ccccggtgtt ggccggcgca gggcgtaacg tcagggtgac
    37801 tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg atacgtttaa gtgtcctttg
    37861 tttgatcatc gtattatctc gccaaattac ctatccaacc gaagtgtact atacattcgg
    37921 cgggccagtt tagcacaaag agcctcgaaa cccaaattcc agtcaattct taatcagctt
    37981 gcttacgtag gaatgctggg atatccagat aatccggctc tttcgcagtt tgcggcgcat
    38041 tgtcattcac tactttagca accggcttct gctcctgggt cagcggagcc atcccatgct
    38101 gctggtagcg atccatcact ggctgctgaa cctgcttatt ggtcaccaga gtgatttcag
    38161 gacgtttgtc catgccgata cctgtcgcaa caacggttac gcgcagctcg tcattcatat
    38221 ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc ggaagcaaat gcacggatgg
    38281 tgttacctac cgtttcgaac tcatccagac gcaggtcgaa gcccgccgtg atgttaacca
    38341 gcacgccgcg cgcgccagac aggtcgatat cttccagcag cggagaagag atagccattt
    38401 cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc agaacccatc attgcgtagc
    38461 ccatttcaga cattacggtg cgtacgtctg caaagtccac gttcatcaaa cccggacgag
    38521 taatcagttc ggcgatacct tgcacagcgc ctttcagtac atcgttcgct gcgccaaacg
    38581 catccagcag ggagataccg cggcccagaa ctttcagcag tttgtcgttc gggatagtga
    38641 tcagagagtc cacatgcttg gacagttcag tgatcccctg ctcggcgaat gccatacgct
    38701 tcttgccttc aaagttgaaa ggcttagtga cgacagcaac ggtcaggata cccaaatctt
    38761 ttgccacttc agcgacgact ggtgctgcac ctgtaccggt accaccaccc atacccgcag
    38821 caataaagac catgtctgca ccttccagcg ccgcacgcaa tgcatcgcga tcctcatcag
    38881 ccgcattgcg gccaacttct ggattagcgc cagcgcccag tcctttggtg ataccgctac
    38941 cgatttgaat cgtctgtcca accgctgttt tacgcagcgc ttgtgcatcg gtatttaccg
    39001 cgaagaattc aacaccttca atacgctcgc gcaccatgtg ttcaacagca ttaccgccgc
    39061 cgccgccgac gccgatgact ttaatcaccg cgtcattggt aagttccatt ggttcaaaca
    39121 tagtttctct ccgatttgtg cctgtcgcct gagtccgtaa tcatcgtcgg cctcataaaa
    39181 attaaaactc ttttcgcagc caactattga gtcgcttgat ccacgagcca actgatgctg
    39241 taacacgttt ttctacttca gcttcaccgt taagatgtga ctctttccca tagtgaagca
    39301 atcccaccgc cgtcgaataa tacggctcct gagcataatc cgttaaaccg gtaatgttca
    39361 gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg agcacaggct gcaagacctt
    39421 cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc caggtgatgt ttaacccctt
    39481 gttggcgaag cttttcctgc aactgcaata tctcttcgtt gaccaggttg agcaactcgg
    39541 tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg cagactccgc ggcggacgac
    39601 cgcctacgct cggcacttcc acgctctcat cttttccaac gatggaaccc agcgcacaac
    39661 cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt gccaaaggcg taagcgatat
    39721 cactggtcac gacattgcca gcataaggaa ttacctttgt gtggcgcaat gccccaccgg
    39781 tataaacggc gatatccatt gtaccaccac cgatatcgac gacgcagaca cccagttcac
    39841 gttcatcttc cgtcaatacc gaataacttg atgccagtcc ggcaaatatc agttggtcaa
    39901 ctttcagccc acaacgttca accgctttga cgatgttttt cgccatatcg ttgtgacatg
    39961 tgatcaggtg cacttttgcc tgcatccgca cacccgaaag tcctaccgga ttcttgatcc
    40021 cttcctgata gtcaatcgca tactcttgcg ggatcacatg cagcacacga tgctcatcgc
    40081 gcacacgcac cgatttcgcg gtatggacga cgttttccac atcttcttgc gtcacttctt
    40141 cttcagaaat aggcaccata ccaatttcat tctggcagct gatgtgctta ccagaaagcg
    40201 ccagatatac cgaagagatc tgacaatctg ccatcaattc tgcctggtca atggcgcgtt
    40261 gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc tttatccata ccacgtgacg
    40321 ggcagctgcc cacgccaatg atattgacca taccgtcggg cagaacttcc cctactaaag
    40381 cggcaacctt cgcggtacca atttccagtc ctactaccag ttttctgtcc gtcgccttga
    40441 tcattgttgt tctgcctgtg cctgattttg ttgctgagta gattcctctg gcggcaaggg
    40501 cgcccagcct actgccgctc cagagtcata acgcaaatca acgtagctaa tccgtttgcc
    40561 atcggtttgc gcctgctgct gtaaaaccgg ataaagttct acaaagcgaa ccaaacgttt
    40621 catcgtatcg ccccggccaa gattgagctt aatatcgtta ttcagcgtca actgccagga
    40681 acgccgcgcg gtcatcgccg cttccttcag agtaaatctg tcctttgcca gcatctgccc
    40741 catttcgcga tagccctgca acacttcatt agcgctgcct tccgggccat acagcattgg
    40801 aagcacctgc ttgctggtgc gatctggcgg cacgctgaag gtgtttcctt ccgcgtctac
    40861 catgtgttga tcattccacc gcgcaatcgg cacatattca accagatgaa tcttcaattc
    40921 atcaggccac tgctttctga cgctcacctg cttaatccac ggcaggcgtt gttctatttg
    40981 cgtctggatg atgttgacat cctgggtcat aaaggtaccc ggctcaccca atgccaggat
    41041 cgactgccgg atatcgtcat tacgcgtgta atggcgttca ccggtcaaca ccagctttga
    41101 gagcggcagg cgttgcgcat cttccatcca gctcaacacg acccagccgc tcaccaacac
    41161 tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga cgcgttccat tattgcggcg
    41221 agaagaaacc tcttcttcgc tgtttcgcgt gttcagagca gcctgcgaca tattagtccg
    41281 ccagttccag aattcgtact accaactgcg agaagctcat acctgcctga cgtgccgcca
    41341 tcggcaccag gctgtggctg gtcatacccg gtgaggtatt ggcttccagc agataaaact
    41401 gtccatcact gtccagcata acgtcaatac gtccccatcc tttgcaacct aacgtcgtcc
    41461 atgctttcag cactaatgcc tgcaaattgg cctcttgtga cgcttccaga cctgcggggc
    41521 agaaatactg tgtctcatca gagagatact tcgcctcata atcatagaag gttccggacg
    41581 gttgaatacg tattgacggt aaaatttctt caccgagtat cgcaaccgtg aactccggcc
    41641 cacttagcca tttttcaatc aatacttctt catcgtgctg aaatgccaat cttaatgcat
    41701 cttgtagagc attttctgct actacttttg acattcccac actggaacct tcgcggctcg
    41761 gcttaacgat aaccggcaaa cccagagcag aaatttctgc taactgctta tcgctcaggc
    41821 ctttttcaaa ctctgcgcgg gttaacgcta cccacggcgc gaccggtaaa ccggcacctt
    41881 gccacagaag tttgctgcgt agtttatcta ttgaaagcgc agatgccatc actccgctgc
    41941 cggtataagg caagcccatc agctcgagca tgccctgcag cgtaccatct tcaccgccgc
    42001 gaccgtgtag cgcgataaac actttctgaa aacccatcga cttcagttgc gtcacgtcga
    42061 cttctttcgg gtcgacagga tacgcatcaa taccgccttc acgcagcccg gctaacaccg
    42121 ctgcgccaga attcagagaa acttcccgct cagcggaagt cccgcccaac aggaccgcga
    42181 ttttatcagt catgttgttc ttcctccgga gtttgcggct tcagtttgat ttcagctaaa
    42241 gaacgggcaa tttttccgat attaccagcc ccctgaacga gaatcaggtc gttaccggtt
    42301 aataccggtg ccagcatctc ggctacctgc gcagggtccg gcaccagaat gggatcaatt
    42361 ttcccacgtc cacgaattgt gcgacacagc gaacggctgt ccgctcccgg aattggcgct
    42421 tcgccagccg gatacacttc cagcatcaac agggtatcaa cctgcgtcag cacattggcg
    42481 aaatcatcat acaggtcgcg cgtacgggta aaacggtgcg gctgaaacag cattaccagg
    42541 tttttatccg gccagcctgc gcgcgccgct ttaatggtgg cgtcaacttc cgtcgggtgg
    42601 tggccgtagt catcgaccag cattgccgta ccgcttttac cattcactgg ctccagcggg
    42661 aattcaccga ggaaatcaaa acggcgacca gtcccctgga agctctccag cgcacgcaaa
    42721 atagcctcgt cgtcaattcc ctcttccgta gcaaccgcaa ccgcagctgc ggcgttcagc
    42781 gcgttatgac ggcctggcgc attcagggtg acgcacatcg gctctttgtc ctgacgcagc
    42841 agcgtaaagt gcccctgcgg gccaatctgc tgataatctt ctacacgcac gtcggcatct
    42901 tcgctgaagc cgtaagttgt ggtctgacgt cccacacgcg gtaacaattc gcggatcacc
    42961 ggatcatcaa cacacatcac cgcacgaccg taaaaaggca ggttgtgcag aaaattaata
    43021 aaggtctgtt ttaaattctc aaagtcgccc tggtaggtat ccatgtggtc ggcttcgata
    43081 ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg atgcatcact ctcatctgct
    43141 tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa cccccgccgc ttttaccagc
    43201 ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt agatgctgga aaccatcgcg
    43261 gtagttgtcg ttttgccgtg cgttcctgca atggcgatgc catgacgaaa acgcattaac
    43321 tcagccagca tttcggcacg acggatcacc ggaatacgcg cttcatgagc tgcgacaatt
    43381 tccgggttgt cggcagaaat ggcgctggaa acaacgacca cgctggcatc acgtacgttt
    43441 tccgggcgat ggttgaaata aatcgtcgca cccagattca gtaactgctg cgtgaccgga
    43501 tttggcgcta aatcggaacc actgatctga taaccttcat tggccagaac ttcggcaata
    43561 ccgcccatac cggcaccacc aatgccgaca aaatgtatgt gccgaacgcg acgcatttcg
    43621 ggcacgatgg aacgcagttt tgccaattgt tgtgtattca ttctttacgc cattaacttc
    43681 ttaaattcat acgatgcaaa aggcatcgct acaattacgc ccgggcagcc cggctcactt
    43741 catttgccac tcgctcggtg gcatccggaa tggatgcagc gcgggcgcgt tctgccatgg
    43801 ttaataaggt ttctcgcgac cacccggcca gggtgttggc gacagcatcc acgctaagct
    43861 gtggctgctc gataattttg gctgcgcccg ctttttccag cggtagcgca ttccagtatt
    43921 gctggcggtc tttatgttga aacggcacaa acaacgccgg tagtcctgcc gcggcgattt
    43981 cactcacagt taaagcaccg gagcggcaaa cgacgacatc cgcccacgca tacgccgccg
    44041 ccatatcatc aataaattca gtcactttat gctgcggttg ccccgcttcg gcatacgcct
    44101 gttcaacgga ttgttgcgaa cctttgccgc tctgatgcca gatagtgact gaatcaccca
    44161 gtttcgcagc aacctgcggc attgtctggt taagaatgcg tgcgccctgg gaaccaccca
    44221 ctaccaacac acgaaccgga ccttcacgtc cagccaaacg ttgctgcggc aacggcagcg
    44281 ccagcacatc ggtacgcacc gggttaccca ccacttccgc attagggaaa gcgcctggaa
    44341 acgcctgcat cactttggta gcaatcttcg ccagccattt attggttaag cccgcaatac
    44401 cgttttgttc atgaagtaca accggaatgc ctaacgacca cgcggccaga ccacctggac
    44461 ctgatacata gccgcccata ccgagtacca cgtcaggttt gtacgctttc ataatcgccc
    44521 gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc tatcagtgct tttatacctt
    44581 ttccacgcaa accagagata cgaatgaaat caatttcgat gccatgtttt ggcactaagt
    44641 ccgcttccat acggtcggca gtccccagcc agcgaacttg ccaaccctga gccattagat
    44701 agtgcgcaac cgccagtccc gggaatacat gtccaccggt tccgcctgcc atcaccatta
    44761 atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac gcctgcgctt tctccagacg
    44821 cgtttcataa tcaatacgca acagcatcat gatggctgtc gacataatca gtaagctcga
    44881 accaccgtaa ctgattagcg gcaatgtcag acctttggtc ggtaacatcc ccgccgccgc
    44941 gcctacgtta accagcgcct ggaagctaaa ccagatgcca atagaacagg cgagaaaacc
    45001 ggaaaaacgg tggtcaattt ctaatgcttt gcggccaatc gacatcgcac gaaaagcgac
    45061 gaagaatacc attaaaagtg ctagtaccac accgacataa cctaattctt cgccgataat
    45121 ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc agtttttgta ccgaattacc
    45181 taaaccttgc ccccaaagtt cgccgcgacc aaacgccatc agcgattgcg ttaactgata
    45241 gccgctgcca aagggatctt cccacgggtt ccagaatgcg gtaacacggc ggatacggta
    45301 cggttcggcg aggatcagca acacaaccgc tgaaatgccc ataccgataa tggcaatgaa
    45361 ctgccacaat ttcgctcccg ccaggaacaa catcgccagc gtagtcacaa acaacaccac
    45421 caccgtacca aggtctggct gtgccagcag taacactgcc aacaccagaa tcacgcccat
    45481 cggtttcagg aagccgcgca ggttattacg tacttcgtcg cctttacgca ccagatagtt
    45541 ggcgatatag caaaacagcg acagttttgt cagctccgca ggctggatac gcagcaaacc
    45601 gagatcgatc caacgcgatg cccctttaac agcgctaccc actaccagca cgatcatcag
    45661 caggatgata gatccgagca gcatcgtggc actgtagcgt tgccagaact ccatcggcag
    45721 acgcagcgta atgatcgcca gaataaacgc caaaatcaga tagacaccat cacgcttcgc
    45781 gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc attgacgccg aggtcaccat
    45841 gataaagcca atcgccgcga ggccgaaggt cagccacagt aaggtgcgat cgtacatgat
    45901 caggctgtcg gtatcttttt cccgcgagcc catcacccag ccctttagcg ccgtggagat
    45961 ccagaccagg atactgaatc ctggcaggcg cggcattttc aggcgaggga gagataaacg
    46021 catcaaccta actccttcgc cagacgggca aactcattgc ctcgttgttc aaagttcttg
    46081 aactgatcaa ggctggcaca ggccggggag agcagaacca tatcgcccgg ctgaacacgc
    46141 ggagccagca agcgcatagc ctgttccata gtttcggttt gttctgccac ttccgggcgt
    46201 agcgccgcca gctgcgcgcc gtcacgaccg aaacaataca gacgtacgtt atcgccattc
    46261 aggtaacgca ccagtgggct aaagtccgcc gatttaccat cgccgcccag caacaaatgc
    46321 agcgtgccgt ctacgtgcag gccattcagc gccgcttccg tactgccgac gttggtcgct
    46381 tttgaatcgt taatccagcg cacaccgtta tgctccagca ccacttcaaa gcgatgcggc
    46441 agaccggtga aggtggttaa cgctttcagg ctgctggcgc gcggtaaccc tgcggcatct
    46501 gccagcgcca gcgccaccag cgcattggtg tagttatgct gcccggaaag tttcatctct
    46561 ttcacattca gcactttctc gcctttaacc cgcagccagg tttcgccctg ctgatgattc
    46621 aggtgatagt cacccatgtt gacgccaaag ctgacgcagc gttcatccgc accgcgaatc
    46681 ggcattgtta aggcatcatc agcattaacc acgcaaactt tcgcgttttc gtaaatgcgc
    46741 agttttgctg ca
//
LOCUS       DAZXUK010000007        42415 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZXUK010000007 DAZXUK010000000
VERSION     DAZXUK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42415
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.007"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..894)
                     /locus_tag="ACH3L8_000306"
     CDS             complement(<1..894)
                     /locus_tag="ACH3L8_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFT4083593.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAA"
     gene            1230..2432
                     /gene="nupC"
                     /locus_tag="ACH3L8_000307"
     CDS             1230..2432
                     /gene="nupC"
                     /locus_tag="ACH3L8_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFT4083594.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(2482..4594)
                     /locus_tag="ACH3L8_000308"
                     /pseudo
     CDS             complement(2482..4594)
                     /locus_tag="ACH3L8_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(4802..4877)
                     /locus_tag="ACH3L8_000309"
     tRNA            complement(4802..4877)
                     /locus_tag="ACH3L8_000309"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4842..4844),aa:Ala,seq:ggc)
     gene            complement(4917..4992)
                     /locus_tag="ACH3L8_000310"
     tRNA            complement(4917..4992)
                     /locus_tag="ACH3L8_000310"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4957..4959),aa:Ala,seq:ggc)
     gene            5213..5572
                     /locus_tag="ACH3L8_000311"
     CDS             5213..5572
                     /locus_tag="ACH3L8_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFT4083595.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            5574..5966
                     /locus_tag="ACH3L8_000312"
     CDS             5574..5966
                     /locus_tag="ACH3L8_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFT4083596.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(6018..7433)
                     /gene="gltX"
                     /locus_tag="ACH3L8_000313"
     CDS             complement(6018..7433)
                     /gene="gltX"
                     /locus_tag="ACH3L8_000313"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFT4083597.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            7692..7767
                     /locus_tag="ACH3L8_000314"
     tRNA            7692..7767
                     /locus_tag="ACH3L8_000314"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7725..7727,aa:Val,seq:tac)
     gene            7812..7887
                     /locus_tag="ACH3L8_000315"
     tRNA            7812..7887
                     /locus_tag="ACH3L8_000315"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7845..7847,aa:Val,seq:tac)
     gene            7892..7967
                     /locus_tag="ACH3L8_000316"
     tRNA            7892..7967
                     /locus_tag="ACH3L8_000316"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7925..7927,aa:Lys,seq:ttt)
     gene            7998..8123
                     /locus_tag="ACH3L8_000317"
     ncRNA           7998..8123
                     /ncRNA_class="other"
                     /locus_tag="ACH3L8_000317"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8243..8584
                     /locus_tag="ACH3L8_000318"
     CDS             8243..8584
                     /locus_tag="ACH3L8_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFT4083598.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(8575..9501)
                     /locus_tag="ACH3L8_000319"
     CDS             complement(8575..9501)
                     /locus_tag="ACH3L8_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFT4083599.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            9591..10589
                     /locus_tag="ACH3L8_000320"
     CDS             9591..10589
                     /locus_tag="ACH3L8_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFT4083600.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLVHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(10586..10804)
                     /locus_tag="ACH3L8_000321"
     CDS             complement(10586..10804)
                     /locus_tag="ACH3L8_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFT4083601.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(10806..12821)
                     /gene="ligA"
                     /locus_tag="ACH3L8_000322"
     CDS             complement(10806..12821)
                     /gene="ligA"
                     /locus_tag="ACH3L8_000322"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFT4083602.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(12892..13878)
                     /gene="zipA"
                     /locus_tag="ACH3L8_000323"
     CDS             complement(12892..13878)
                     /gene="zipA"
                     /locus_tag="ACH3L8_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFT4083603.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            14108..14869
                     /gene="cysZ"
                     /locus_tag="ACH3L8_000324"
     CDS             14108..14869
                     /gene="cysZ"
                     /locus_tag="ACH3L8_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFT4083604.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            15054..16025
                     /gene="cysK"
                     /locus_tag="ACH3L8_000325"
     CDS             15054..16025
                     /gene="cysK"
                     /locus_tag="ACH3L8_000325"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFT4083605.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            16409..16666
                     /gene="ptsH"
                     /locus_tag="ACH3L8_000326"
     CDS             16409..16666
                     /gene="ptsH"
                     /locus_tag="ACH3L8_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFT4083606.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            16711..18438
                     /gene="ptsI"
                     /locus_tag="ACH3L8_000327"
     CDS             16711..18438
                     /gene="ptsI"
                     /locus_tag="ACH3L8_000327"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFT4083607.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            18479..18988
                     /gene="crr"
                     /locus_tag="ACH3L8_000328"
     CDS             18479..18988
                     /gene="crr"
                     /locus_tag="ACH3L8_000328"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFT4083608.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(19030..19881)
                     /gene="pdxK"
                     /locus_tag="ACH3L8_000329"
                     /gene_synonym="thiJ"
     CDS             complement(19030..19881)
                     /gene="pdxK"
                     /locus_tag="ACH3L8_000329"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFT4083609.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            19986..20360
                     /locus_tag="ACH3L8_000330"
     CDS             19986..20360
                     /locus_tag="ACH3L8_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFT4083610.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            20393..21116
                     /locus_tag="ACH3L8_000331"
                     /pseudo
     CDS             20393..21116
                     /locus_tag="ACH3L8_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(21320..22231)
                     /gene="cysM"
                     /locus_tag="ACH3L8_000332"
     CDS             complement(21320..22231)
                     /gene="cysM"
                     /locus_tag="ACH3L8_000332"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFT4083611.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(22365..23462)
                     /gene="cysA"
                     /locus_tag="ACH3L8_000333"
     CDS             complement(22365..23462)
                     /gene="cysA"
                     /locus_tag="ACH3L8_000333"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFT4083612.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(23452..24327)
                     /gene="cysW"
                     /locus_tag="ACH3L8_000334"
     CDS             complement(23452..24327)
                     /gene="cysW"
                     /locus_tag="ACH3L8_000334"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFT4083613.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(24327..25160)
                     /gene="cysT"
                     /locus_tag="ACH3L8_000335"
     CDS             complement(24327..25160)
                     /gene="cysT"
                     /locus_tag="ACH3L8_000335"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFT4083614.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(25160..26176)
                     /gene="cysP"
                     /locus_tag="ACH3L8_000336"
     CDS             complement(25160..26176)
                     /gene="cysP"
                     /locus_tag="ACH3L8_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFT4083615.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(26480..27271)
                     /gene="ucpA"
                     /locus_tag="ACH3L8_000337"
     CDS             complement(26480..27271)
                     /gene="ucpA"
                     /locus_tag="ACH3L8_000337"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFT4083616.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(27400..28258)
                     /gene="murR"
                     /locus_tag="ACH3L8_000338"
                     /pseudo
     CDS             complement(27400..28258)
                     /gene="murR"
                     /locus_tag="ACH3L8_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
     gene            28422..29318
                     /gene="murQ"
                     /locus_tag="ACH3L8_000339"
     CDS             28422..29318
                     /gene="murQ"
                     /locus_tag="ACH3L8_000339"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFT4083617.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            29322..30746
                     /gene="murP"
                     /locus_tag="ACH3L8_000340"
     CDS             29322..30746
                     /gene="murP"
                     /locus_tag="ACH3L8_000340"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFT4083618.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            30751..30837
                     /locus_tag="ACH3L8_000341"
                     /pseudo
     CDS             30751..30837
                     /locus_tag="ACH3L8_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(30926..31825)
                     /gene="yfeX"
                     /locus_tag="ACH3L8_000342"
     CDS             complement(30926..31825)
                     /gene="yfeX"
                     /locus_tag="ACH3L8_000342"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFT4083619.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(31921..32496)
                     /locus_tag="ACH3L8_000343"
     CDS             complement(31921..32496)
                     /locus_tag="ACH3L8_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFT4083620.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(32557..33006)
                     /locus_tag="ACH3L8_000344"
     CDS             complement(32557..33006)
                     /locus_tag="ACH3L8_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFT4083621.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(32993..33418)
                     /locus_tag="ACH3L8_000345"
     CDS             complement(32993..33418)
                     /locus_tag="ACH3L8_000345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFT4083622.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            33632..34501
                     /gene="amiA"
                     /locus_tag="ACH3L8_000346"
     CDS             33632..34501
                     /gene="amiA"
                     /locus_tag="ACH3L8_000346"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFT4083623.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            34505..35404
                     /gene="hemF"
                     /locus_tag="ACH3L8_000347"
     CDS             34505..35404
                     /gene="hemF"
                     /locus_tag="ACH3L8_000347"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFT4083624.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(35410..36462)
                     /gene="eutR"
                     /locus_tag="ACH3L8_000348"
                     /pseudo
     CDS             complement(35410..36462)
                     /gene="eutR"
                     /locus_tag="ACH3L8_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(36508..37008)
                     /gene="eutK"
                     /locus_tag="ACH3L8_000349"
     CDS             complement(36508..37008)
                     /gene="eutK"
                     /locus_tag="ACH3L8_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFT4083625.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDIQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(37021..37680)
                     /gene="eutL"
                     /locus_tag="ACH3L8_000350"
     CDS             complement(37021..37680)
                     /gene="eutL"
                     /locus_tag="ACH3L8_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFT4083626.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(37690..37758)
                     /locus_tag="ACH3L8_000351"
                     /pseudo
     CDS             complement(37690..37758)
                     /locus_tag="ACH3L8_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            38862..39653
                     /locus_tag="ACH3L8_000352"
     CDS             38862..39653
                     /locus_tag="ACH3L8_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4083627.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            40373..40768
                     /locus_tag="ACH3L8_000353"
     CDS             40373..40768
                     /locus_tag="ACH3L8_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFT4083628.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            40786..41049
                     /locus_tag="ACH3L8_000354"
     CDS             40786..41049
                     /locus_tag="ACH3L8_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4083629.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            41072..41614
                     /locus_tag="ACH3L8_000355"
     CDS             41072..41614
                     /locus_tag="ACH3L8_000355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4083630.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            41753..42040
                     /locus_tag="ACH3L8_000356"
     CDS             41753..42040
                     /locus_tag="ACH3L8_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4083631.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            42079..42285
                     /locus_tag="ACH3L8_000357"
     CDS             42079..42285
                     /locus_tag="ACH3L8_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4083632.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
BASE COUNT        10743 a        11147 c        10383 g        10142 t
ORIGIN      
        1 ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca gcggttgcag
       61 cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt ggaacgccgc
      121 cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg cgatagccac
      181 atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag
      241 cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc ctgctgccag
      301 gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca gctgcgccag
      361 gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca acagtaaccc
      421 gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt
      481 cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga gtttaaaacc
      541 gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt ctgcctgaac
      601 ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg ccagattttt
      661 accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca tcaggttggc
      721 ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa tattggtcgc
      781 aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag
      841 cctcatctta cgcgccgccc gtccgctgct actctcaacg cgatagttcg tcatcttgtg
      901 cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg attatcaaat
      961 tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta cgaaaagatt
     1021 gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact ttcagcttcc
     1081 atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt cacaggacgt
     1141 cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa
     1201 atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt gtactggcac
     1261 ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc
     1321 gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc ctgaactccg
     1381 acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg
     1441 ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg gcattcttct
     1501 tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt ctccagcaca
     1561 ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa gtcaacggta
     1621 tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag tcagaaaact
     1681 ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac accatggctg
     1741 ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc atgctggaac
     1801 cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc gtgctgtcgc
     1861 tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc aacctgcacg
     1921 aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc aaagttgcca
     1981 ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg aacgcactgt
     2041 ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc ctgggctaca
     2101 tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg caagtgggca
     2161 gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat ctgcagaaaa
     2221 ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta gtttccttcg
     2281 ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat gaagagcaag
     2341 gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc
     2401 tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc cggggatcat
     2461 tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa tcaaataccc
     2521 ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa
     2581 ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc tcacaatcat
     2641 cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt tgatgatatc
     2701 agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc
     2761 aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt ctgcgttaga
     2821 aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt gataacgctt
     2881 aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg gcattaaatt
     2941 gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact tcaacaagga
     3001 ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa
     3061 cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct catcataacc
     3121 ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct gatctaacgc
     3181 cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa ccatctcctc
     3241 tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca gccagcttaa
     3301 ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc aggcagcacc
     3361 atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca ggatgttaac
     3421 catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca gttcactacc
     3481 cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac ggcaaattga
     3541 gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact caagattatc
     3601 aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt ccaacgcacg
     3661 aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc ggcgattaag
     3721 ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa aggaaatcag
     3781 caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa agttctgatt
     3841 gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag
     3901 attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac cggcaataaa
     3961 gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg ccagccagct
     4021 taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact gcggtgaaaa
     4081 ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtggatatct
     4141 ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg ccgatcggcg
     4201 tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat ccataacgcc
     4261 aacgcgtccc cagctgccag cgtaatattg cgcaagcaca caacacgaca aacgtctggc
     4321 tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa
     4381 acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag attgacagca
     4441 ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg gcaagtggcg
     4501 aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct
     4561 tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg
     4621 ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt
     4681 tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac gtagactgta
     4741 ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag ggttggaaat
     4801 ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa gcgctctccc
     4861 agctgagcta tagccccacg atgcgtttac gtaccaaatt tgctgggtgc aaaatttggt
     4921 ggagctaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg
     4981 agctatagcc ccgtcacgta aagcttgtcg agttgacggg cggcatcata tgaattccgc
     5041 ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat cgctgaaaaa tcaggcaaat
     5101 gaacaatttt ggaaaaccgc tcgcattcag tagttattca tgtcacggtt tcctgtaaag
     5161 tggtgttata aaatgaacta ctaatagacc cacatacatt cagggaattg ttatgttcaa
     5221 ggagaggatg acaccagatg aacttgccag actgaccggc tatagccgcc agaccattaa
     5281 taaatgggta cgcaaggaag gctggacaac gtcaccaaaa cctggcgtcc agggtggcaa
     5341 agcccgactg gttcacgtca atgaacaggt tcgtgaatat attcgcaatg ctgaacgtcc
     5401 agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg ccgcttgaag tattgctggt
     5461 gacactggcg aaagagatga cgccagttga acaaaaacag tttacatcct tgcttctgcg
     5521 ggaagggatt atcggattgt tacaacgctt agggattcgc gatagcaaat aatatgaaaa
     5581 gattacgcaa taaaatgacc accgaagaac tggctgaatg cctcggtgtg gctaaacaaa
     5641 ctgttaaccg ttggaccaga gaaaaaggct ggaaaacgga aaaatttccc ggcgtgaaag
     5701 gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg cgagtttatt cagaacacgc
     5761 cagccttcca taacacacca atgttgatgg aagctgaaga gagaattgct gaatacgctc
     5821 ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc gatagataat atgacagaca
     5881 ttgagcagga aaaagtcgcg caatttttgt cacgtgaagg aattcgcaat ttcctcgccc
     5941 gtctcgatat tgacgaatca gcataaataa aaaacggcag gatattatct cctgccgttt
     6001 atctttttac acgctaatta ctgctgattt tcgcgttcag caataaaatc cagcgctttg
     6061 ttgatacgct cgatactgcg ggtcttacca attgcgtgaa cggtaacatc cagtgctgga
     6121 gactgccccg caccggttac ggcgacacgc agcggcatac caactttacc catacccact
     6181 tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt tttcagcggt ccagtcagta
     6241 atcgcggcca gtttgtcacg aaccacttcc agcggctgac gcgctaccgg acgcagatgt
     6301 tttttcgccg catcggcatc gaactcagca aaatcttcgt agaaataacg gcagctctgt
     6361 gccatctctt tcagcgtctt gcagcgttcg cccagcagtt tcaccagatc agccagctgc
     6421 gggccgttac gggtatcgat attttcctgc tcaatgtgcc actgtaagtg agtagcaaca
     6481 tactccggcg gcagcgcgtt aatgtagtga tggttcagcc acagcagctt gtcggtgttg
     6541 aacgcactgg cagatttgct gacggcattc aaagtgaagt atttgatcat ctcttcacga
     6601 gtgaagattt cctgatcgcc gtgggaccag cccagacgca ccagatagtt cagcagtgct
     6661 tctggcaaat aaccgtcatc acgatactgc attacgctga ctgccccgtg acgtttggac
     6721 agttttttac cgtcatcgcc attgatcata gaaacgtgcg cgtaaaccgg caccggcgct
     6781 ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt tgatatggtc ttcgccacgg
     6841 ataacgtggg tgatttccat atcccagtca tcgacaacca cacagaagtt ataggttggg
     6901 gaaccatcgg tacggcggat aataagatcg tccagttcct ggttgctgaa ctcgatcgga
     6961 ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg ggttagcaaa acgtacaaca
     7021 cacggttcat catcagcatg atgctcatgg ctgtggcggc agcgaccgtc ataacgcggc
     7081 ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca ggcgctcttt agagcaatag
     7141 catttataag cagtgccctc ttccagcatc tgatcgatca ccgcgttgta gcgatcaaaa
     7201 cgtttggtct ggtagtacgg accttcatcc cactccaggc tcagccagtt catgccatcc
     7261 ataatggctt cgatagcttc cggcgtggaa cgctcaagat cggtgtcttc aatacgcagc
     7321 acgaactcac cgccgtggtt acgtgcaaaa agccaggagt aaagagcagt acgcgcgccg
     7381 ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag ttttgatttt catgaaatgg
     7441 ccttacgttt agaaagatgc cgacaaccgg caaatcctgg aaaaattgag tgggcgatat
     7501 tctatcactc ccgcctgatt cctcaatgta gttcgggttt ctaccttacg cattattgtt
     7561 ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca tcttgcttaa atttacgacg
     7621 aacgaacaat ttctttagaa aatgcgttga ctcattttga actctcccta taatgcgact
     7681 ccacacagcg ggggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
     7741 gcggttcgat cccgtcatca cccaccaact actttatgta gtctccgccg tgtagcaaga
     7801 aattgagaag tgggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
     7861 gcggttcgat cccgtcatca cccaccactt cgggtcgtta gctcagttgg tagagcagtt
     7921 gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatg taaaaaagcg
     7981 ccctaaaggc gcttttttgc tatctgcgat actcaaagat tcgaacctgc agcaggtttg
     8041 agttgagcgc agcgaaacaa cggagccgct cgcggcgacg gcccgaaggg cgagcgaagc
     8101 gagtcatcct gcacgaccca ccaatgcaaa aaagcgccct aaaggcgctt ttttgctatc
     8161 tgcgatttgt aagattgatt tttcttctca agttccttgt tcttctaccg atattacctc
     8221 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
     8281 aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat
     8341 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
     8401 tgctgaagac aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
     8461 acaactggcg cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt
     8521 gtctctgaat gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat
     8581 ctgatacaac ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
     8641 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
     8701 ctggcgttca accaccggcg taatgcgctt aaccaccagt gggctacctt caggcaatgg
     8761 tagcgccagt gcaggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
     8821 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
     8881 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
     8941 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
     9001 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
     9061 gatgccaaag tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
     9121 atgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
     9181 aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc
     9241 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
     9301 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
     9361 aacaccagta tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc
     9421 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
     9481 ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat
     9541 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
     9601 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
     9661 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
     9721 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc
     9781 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
     9841 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
     9901 acttgtgcat tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
     9961 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt
    10021 caccattgct ggttggtctg gtgatgaatg ttcacggtgc aaggggcagc cttgagcagg
    10081 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggtgcatctt tcccggccgt
    10141 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
    10201 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
    10261 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
    10321 ttgtagttaa cgtctttatg gcacgccgac tgagcttcaa taaggcagat gaaattacta
    10381 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
    10441 tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat
    10501 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
    10561 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
    10621 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
    10681 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtca attaagcgac
    10741 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
    10801 ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
    10861 tcctgcgcct tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc
    10921 ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca
    10981 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
    11041 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
    11101 ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca
    11161 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc
    11221 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
    11281 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
    11341 tgtgccaatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
    11401 agatctgccg gagtatggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
    11461 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
    11521 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
    11581 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
    11641 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
    11701 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
    11761 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
    11821 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
    11881 ttaaacgcaa ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
    11941 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
    12001 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
    12061 ctgaccggca ccccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
    12121 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
    12181 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
    12241 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
    12301 acttcaccac gcacttccag acgcgccggg atattctctc cgtgcagctt cagcggaatg
    12361 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
    12421 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
    12481 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
    12541 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
    12601 cgtatctggc tgaaagctgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
    12661 gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
    12721 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcaaagc
    12781 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
    12841 aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt
    12901 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
    12961 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
    13021 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt
    13081 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
    13141 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
    13201 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
    13261 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
    13321 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
    13381 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
    13441 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg
    13501 ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
    13561 ctggaccggt tgacgcggct gcgcagacgc ataaggcggt aggtactggt gttgcggcga
    13621 cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg
    13681 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
    13741 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt
    13801 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
    13861 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
    13921 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
    13981 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
    14041 ttgcgcaatt acccggtaaa atacgcagaa ttttcctggg attggtcaaa aggagctcat
    14101 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
    14161 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
    14221 caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat
    14281 cccgactttc atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc
    14341 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
    14401 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
    14461 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
    14521 cgaatggcaa aaatttgtct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt
    14581 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
    14641 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
    14701 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct
    14761 gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac
    14821 ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
    14881 ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt
    14941 gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc
    15001 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
    15061 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
    15121 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
    15181 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
    15241 ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg
    15301 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
    15361 tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg
    15421 caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
    15481 aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg
    15541 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
    15601 ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg
    15661 ttgagccaac cgattctcca gttatcgccc aggtgctggc aggtgaagag attaaacctg
    15721 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
    15781 tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa
    15841 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc gcagcgttga
    15901 aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg
    15961 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
    16021 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc
    16081 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
    16141 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
    16201 tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc
    16261 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa
    16321 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
    16381 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttaccgctcc
    16441 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
    16501 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
    16561 gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca
    16621 gaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
    16681 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
    16741 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
    16801 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
    16861 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
    16921 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
    16981 gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt
    17041 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
    17101 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
    17161 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
    17221 ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
    17281 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
    17341 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
    17401 ccaaccaacg aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa
    17461 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa
    17521 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
    17581 ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct gcccactgaa
    17641 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
    17701 gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
    17761 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg
    17821 cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
    17881 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
    17941 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
    18001 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
    18061 atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt
    18121 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    18181 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    18241 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    18301 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    18361 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    18421 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    18481 gggtttgttc gataaactga aatctctggt ttccgacgac aaaaaggata ccggaactat
    18541 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    18601 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    18661 cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga
    18721 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    18781 cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat
    18841 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat
    18901 ctccaacatg gacgaaatca aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga
    18961 aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgcccatcgg
    19021 cgccattttt tatgcttccg ccagcggcgg caaaatcaat tcatcgctct catgctgctg
    19081 ggtgtagcgc atcacttcca gtacgcgcaa ccccgcacgg tgcaccgcat cgtttaacgc
    19141 cttccctttc agcaaaccac tgataagctg agcacaaaac aggtcgccag tccctttcag
    19201 gtcggttttt acccgtgaat gggaaatgac attcacgctg tcggcactga ccaccaccac
    19261 ctgcatctcc tgattttctt cattaccgga ggcgctggta atcaccaccc attttaatgt
    19321 gtctgaaagc agactttttg cggcagcaat ggcactgtcg agatcgcggc aatttttacc
    19381 ggtcaggatt tccagctcaa agatattggg ggtaattccc tgcgccagcg gcagtaaata
    19441 ttgtcgatac gcctcgggaa ggtcaggttt gacataaatt ccgctatcaa tatcgccaat
    19501 caccggatcg accatgatca ataggtcagg atggtctttg cgtagcgcag tcagccactc
    19561 ggcaaggatt ttgatttgcg atgccgttcc catatagccc gtggttacag cacgaagttg
    19621 gcgcagcgca tcacgctcct gaagcgcacg caaatagccg ctaaaccatt cgtccggaat
    19681 cgcaccaccg tagaaagtgt cataatgcgg cgtattgctc agcaataccg tcggcacggc
    19741 aaagacattc aagccgttct gttttatagc aggcaccgca atgctgttgc ctacgcttcc
    19801 gtacactacc tgcgactgca cggcgacgat atccgcctgc agcgccctac tcttatcgtt
    19861 aaacaacaac aaactactca tttaattttt tctccttgcc gatgatcctc atcgtattcc
    19921 aaccgaaact ttacctgatt ctggcagtca aatcggctat tacaaaacaa agataaggta
    19981 attcagtgaa gaaaatcatt tgtctggcca ttacgctgct aatgacactc cccgcttacg
    20041 cgaagttaac tgttcatgaa gaagcccgca tcaacgccat gctggaggga ttagcacaga
    20101 aaaaggattt gatatttgtg cgcaacggtg atgaacatac ctgcgatgaa gcggtttccc
    20161 acctgcgtct gaagctcggc aatacccgta accgcattga cactgccgag cagtttattg
    20221 ataaggtagc ttcgtcgtca tcgattaccg ggaagccgta tatcgtgaag atccccggta
    20281 agagcgatga gaacgcacag ccttttttac atgcgttaat tgcgcagacg gataaaacgg
    20341 tgcctgcgga aggaaattaa tccgctttgg taaggcattt acaggaggta acatgaaaaa
    20401 atgctttatt tatcacgatg aaaaatcgaa taaattttgg tggatagatt acgaagggga
    20461 tagtttagct gtcaactatg gcaaggtagg tagtattggt aaattccata caaaagaatt
    20521 cgataatgaa gaacagtgtc tgaaagaagc cagtaaattg attgccgcaa aaatgaaaaa
    20581 aggatatcaa gaaactccag actttaactt catggatctc tactattttg atgatgaaga
    20641 gattgggtta catcttaaaa cgtcacaccc aaacttccag tgccatttta ctgatccact
    20701 ttatatgtgt tgctgggatg aagaatctcc ttttggtagc gatgaaggtg ctgatgctct
    20761 aaacgaactt gaaaatagcc tccgttctgg actgtgctga tttccctcaa atgttaattg
    20821 aaactatgtg gggtatgaaa tacatcgcta tggacagtat tcttgaagag gatgtgcgtg
    20881 cgcaattact agccgatgaa atgagctcta tccagagcaa tatgattacc tacgcgactg
    20941 cattcggtca gatcaaagtc atgggtaaaa tctcccataa acttaaaaag atgggactca
    21001 atgcactagc gcgtcatcag cttaccgcaa aaattcttca atggggtgac ggtcaggact
    21061 caccaatact tcaaaaaatg attgatgacc ttactgcttt tccacacgaa aattaaaaac
    21121 tgcatttgtc ggcagcaaca actgccgaca aatcagatta ttgacaaagt gcactttgtt
    21181 tatgccggat gcggcgtaaa cgcctgatcc ggcataaaaa tattgctaat tcaatatatt
    21241 gcagaaacat tgtaggcctg ataagcggag cgcatcaggc agttttgcgt ttgtcatcag
    21301 tctccaatgc tattaatcct taaatccctg ccccctgact aaaatgctct tccccaaaca
    21361 ctccggtgga gaggtagcga tcgccacgat cgcagatgat cgctaccacc accgcaccag
    21421 ggttagcttt tgccacccgc agtgctccag caaccgcgcc gcctgagctg acgccacaga
    21481 atattccttc ccgcaccgcc agttcgcgca tggtgttttc cgcatcgcgc tgatgaatat
    21541 ccagcacctc atccaccagc gcagcgttga aaatccccgg cagatattcc gcaggccagc
    21601 ggcgaatgcc cggaatactg ctgccttctt ccggttgcag gccaacaatg gtcaccggtt
    21661 tggattgttc gcgcataaag catgaaacgc cggtgatagt gccggtcgtc cccatgctgg
    21721 agacaaaatg agtgatgcgc ccgccggttt gctgccagat ttccggcccg gtggtggtgt
    21781 aatgcgcata agggttatcg ggattattga actgatcgag cagctttcct tcgccacgat
    21841 tcgccatctc cagcgccaga tcgcgcgcac cttccatgcc ctgctctttg gtgacaagaa
    21901 tcagttccgc accataagca cgcatcgccg cacggcgttc ctggctcatg ttgtcgggca
    21961 tcagcaattt catgcgatag cctttcagcg cggcaatcat tgccagcgca atgccggtgt
    22021 taccactggt ggcttcgatt aagacatcac ccggtttaat ttccccgcgc ttttccgcct
    22081 cgacgatcat cgaaagtgcc gcacgatctt tcaccgaacc tgccgggtta ttgccttcca
    22141 gttttaacca cacttcactg ccgttatccg gccccattcg ctgcaacttc accagaggcg
    22201 tattgcctat tgtttgttct aatgtactca caatctctat ccatacgtgg tgttgcctga
    22261 tgcgacgctt gcgcgtctta tcaggcctac aggttacaaa ccttgccatt aaaaagcccg
    22321 gatcgcggga agcgcctccg ggcgtttaac attcactcaa cctatcaggc gctttgtgcg
    22381 agagcaagtt cctcatcgcg ggtttcaata cgctcgtcgc cgttatacag ccgcgcatgt
    22441 tgcagaccaa cgaataaacg ctcgccatgc tgcggggcat cgtcgccatg catcacgacc
    22501 gtcagcggtt cgttgtacca ccccagcggc tgcaccacta attgggtgta gtgacctttc
    22561 gggctggctt ccagtacctg taccggcagc ggcgaatcga gactggtacg gcggctgata
    22621 tccacttccc acgggcgcag gaagagatcc accggcccct gatacgcagg tgtgtagccc
    22681 agcggccagc gatgcgcgcc aacatggaac tgcccgccgc gaatggttcc ctgcaggcgg
    22741 ttcacttcgc ccataaattc gagtacaaaa cgggtcgctg gttcgcgcca tacctgattc
    22801 ggcgcatcag cctgttcaat attcccctga ctcatcacca ctacacgatc ggctacttcg
    22861 gtcgcctctt cctggtcgtg ggtcacaaac acgctggtga atttcagctc ttcatgcagt
    22921 tgacgcagcc agcgacgcag ctctttacgc acctgcgcat ccagcgcgcc gaacggttca
    22981 tcaagcagca gaatttgcgg ttctacagcc agcgcgcgcg ccagcgccac acgctgtttc
    23041 tggccgccgg aaagctgcgc cggataacga tccgccagat gcgcaagctg gaccatttcc
    23101 agcaattttg tcactttcgc tttgatggct gcggcattcg ggcgctcgcg acgcggcagc
    23161 accgtcaggc caaaagtgat attgtcgaat accgtcatat ggcggaacag cgcgtaatgc
    23221 tggaacacga aaccgacttt acgatcgcgc gcgtgcaggc gactcacgtc ggtgccgtgg
    23281 aagcgaatat gcccgctggt ttgatgctcc agcccggcga taatccgcag cagcgtggtt
    23341 ttcccggaac cggacggccc cagcaacgcg accatctgac cagaaggaat atccagtgag
    23401 atatcgttca gcacctgggt gcgaccaaaa gacttcttga tattggcaat ctcaatgctc
    23461 atgatgctcc tcctgctgcg cgcgtttttc ctgattctcc aggcgccact gcaacatact
    23521 ctttaaaaac agggtgataa tcgccatcaa cgtcaacagc gcggcggcgg taaaggagcc
    23581 gacggtgttg tagtcctgct ccaacaattc aatctgtaac ggcagcgaaa gtgtttcgcc
    23641 gcgaatcgag ccggaaacca ccgacactgc accaaactcg ccaatcgcgc gggcgttggt
    23701 cagcaccacg ccgtaaagca gcgcccagcg aatattcggc aatgtgacgc gacggaacat
    23761 ctgccagccg gacgcgccaa gcaaaatcgc cgcttcgtct tcctggctgc cctggcttaa
    23821 catcaccggc accagctcgc gcaccacaaa cggacacgtc acaaagatgg tgaccagcac
    23881 cattccgggc caggagaaca taatttgcag attatgttca tccagccagc cgccgagcgg
    23941 gccgttggag ccgtagaaca gcagatacac cagaccggca accaccggcg atacagcaaa
    24001 cggaatgtcc agtagcgtca gcagtaactg gcgtcccggg aagttaaagc gcgtcaccag
    24061 ccaggccagc agaatgccga acaccaggtt taccggtacg gcaatcagcg cgatcatcac
    24121 cgtcagccag atggcgtgca gcatgtccgg atcggccaga ttctgtaaaa ccggcatcag
    24181 ccccttgctg aatgcctgca cgaagatgta aatcatcggc accagcagga tgaacgccga
    24241 aaccagcatc ccgatgccaa tcagaaacca tttgccccag ttaatcgggc gcgcgtcata
    24301 acgcttcaat tgggtaactt ccgccattaa tgacctacca cacgccgacc aaagcgactt
    24361 tgcagagtgt taattgagaa cagcagcagc agagatgccg cgaggatcac cgaagcaatc
    24421 gcgctcgctg ccgggtaatc aaactcctgt aagcgcacaa aaatcatcag cgacgtcact
    24481 tccgtcttcc acgcgatatt tccggcgata aaaatcaccg cgccaaattc accaagacta
    24541 cgggtaaacg acagcgccac gcccgccacc agcgccggag aaagctccgg cagcaccact
    24601 ttgcagaaac tctgccagcg cgttgcacca agcgtttccg ccgcttcttc atattccggg
    24661 cctaactctt ccagcaccgg ctgcacggta cgcaccacaa acggaatgct ggtaaaggcc
    24721 atcgccaccg caataccaag ccaggtatag gtgactttga tatcaaactt cgccagccat
    24781 tcaccgtaaa aaccgtttac ggaaaagagc gaggccagcg ttaaaccggc gactgccgtt
    24841 ggcagcgcaa agggtaaatc catcagcgca tcaagcagcg tgcggcccgg aaagcgataa
    24901 cgtgttaaaa tccacgccat cagcagaccg aaaacgccgt taaaaatcga tgccacaaac
    24961 gccgacagca gcgtcacttt gtaggccgcg accacctgcg ggttggtgat cacctcccag
    25021 tactgcgccc agctcatctc agaaagctgc atcaccagcg cagagagcgg cagcagcaat
    25081 atcaggcaca caaacagcag actggtgcca aggcttaaag taaagcccgg cagcacgcgt
    25141 cttgaagaaa ctgcaaacat tacttacgcc ccgccgctaa cagcttgtct aactcgccgc
    25201 cgctggtgaa gtgggttttc atcacttccg gccatgagcc aaatttgtct tccacgcgga
    25261 acagttcggt ctgcgggaat ttatctttca gtttgtccat gacttccggg ttattcacac
    25321 ggtagtaata gtcggtgatg atggtctgcg cctgtgggct gtagagccag ttcaggtagg
    25381 cttttgcggc tttttccgta ccgttggcct gcacattttt atcgacccac gccaccggga
    25441 attccgccag aatattggtt ttcgggatca ccacttcaaa gccctgtgct tcatactgtt
    25501 tacggatgtt gttcacttcc gattcaaagc taatcagcac atcgcccaag ccacgctcgg
    25561 caaaagtggt ggttgcgcca cgaccgccgg tatcgaacac ttcaacgttt ttcaggaact
    25621 gggtcataaa ctgttcggtt ttggctttat cgccaccgtc agctttatcc gctgcgcccc
    25681 atgccgccag ataggtataa cgcgcgttac ccgacgtttt cgggttcggg aaaatcagct
    25741 tcacgtcgga gcgcaccagg tcgttccagt cgtggatatt cttcgggttg cccttacgca
    25801 ccaggaagcc catggtggag tagaacggcg agctattatt cggcaggcgc gactgccagt
    25861 cagccgggat cagcttgcct ttgtcgtgca ggatttgtac gtcggtcacc tggttataag
    25921 tgacaacgtc tgcttttaag ccctgcaaaa tcgccagcgc ctgttttgat gacccggcat
    25981 gagattgttt tatcgtcagt ttgtcgccgc cgttatcttt tgcccattgc tgttcaaacg
    26041 gcggattcag ggcggcaaac agctcgcggg agacgtcata agaactgttc agcagttccg
    26101 ttgcctgtac atggcccgcc agcagcagag aagcgaccag cgcgagtgag ttctttttca
    26161 gtaagttaac ggccattgcg cacccttata aatttaatga ctttctaata gccatcatat
    26221 ttataacgga cgtgaaagga gtaacggttt tatataccgt ttggtgattt ggaagttaaa
    26281 aagggaataa gaacttggtt caacaggcgt aattgttgca ggcagtttgg acacggacag
    26341 cgcggagaaa ccggagcgta cacatagtac gtgaggattt cgagcactgc ccggggccaa
    26401 aatgacaaat gaaatagcct ggtgaactta gttcaagacg aaatcctccc cacacatgca
    26461 gggaggaaac agaggtgaat cagataccga cgctaaccgt ctccggcagt gtgctgccgc
    26521 catcaatcac attctgtgta ccggttaaat agctggattc atccgatgcg aggaaggccg
    26581 ccagttcgcc gacttccagc ggatcggcga ggcgacacag cgggattgct tttgccattt
    26641 cagtcagcac cgattctgga tcttccgggt tcgactggcg ggcaatgctt tccgccattg
    26701 gcgtgcggac gtatcccggg cagatggcgt taacgcgaat accggattgc gcgtactcca
    26761 ccgccagcga tttagtcagg ccaacaatcg ccgctttcgt taaggcatac gccgtttcgc
    26821 caggatcggc caccatatca ccagtgactg aagacatcat cacaatgcga ccatctttgc
    26881 gcgcaatcat ctccggcagc accgccttcg tgacgttcca tacgccttta atattgatat
    26941 caatatggaa atcgcgatct tcatcgctca tatcgaggaa actgcccaga cgacaaacgc
    27001 ctgcgttatt caccaggata tcaatgcgcc cttctttttc cttcgcgcgt ttgatagctg
    27061 cggctaccga cgccgggtca cgcacatcgg cgacaaccgc cgtacagcga tgaccacgac
    27121 cacacagttc gtccgctagc ttttcgatct caggggagat atccagcaag attaggttcg
    27181 cgccatgacg tgcaaaagtt ctggcaattc cttcgccaat tccctgcaat gcgcccgtaa
    27241 tcagtgctgt cttgcccgtg agtttaccca ttttaatgct ctccttgtta atacagagcg
    27301 ttacactcca ttccctctga aaatacagga taaacaaaat attgctgcgg atcagctcac
    27361 taattcagga aaatctcaat ccagtcgctg ctttatgcat catttcaggc gttgtgttag
    27421 ctcactgctg cgctgaatca ttttcaatga cgccacatca ttgatgttgc acaagaccaa
    27481 caaacagtaa atcggtaaca gaattttgcg ccgtgcgggt agacatcgac gagctacgcc
    27541 attcggtttc accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt
    27601 cagccagaga agtaatggca atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca
    27661 agacaatctc ttttttactg ccgctgtaag agatagcgat ttgtacatcg ccttttttca
    27721 acgcctggga aacggtggct tgtacgtgcg tgtcggcttc gcaggccacg cgatagccaa
    27781 ttttcatcag tttgaacgac agatcgcgcc ccaccagcgc cgatccgccc aggcccgtta
    27841 tctggataaa cggtgcttta ctgatgacct caatgatttt ttgcagccgc gcgtagtcga
    27901 acaacgcaca ggtttgctcc agcgccagct ctttttcgcg attcagtttg cgggcaatca
    27961 cttccagcga atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt
    28021 tctcccggct ggcactgtat tcgccaatta acgccatacg caattcagta aatccttgcg
    28081 cgccgagctt ctgggcaaat ttcacaatgc tcgactggct gataccgagc tgtttcgcca
    28141 tctgccgtga agagacggat ttcagctcgc tcactctggc ccgcagaaag tcagcaattt
    28201 tttgctcgtt cccggtaaat tcactttccg cattgcgtat tttcgtcagg tacaacatct
    28261 tccactctcc tgggctggca gcaaaaattc cgcttaagtt cttatgacgc tcttcacact
    28321 ctgcgagtgt agactcaatg attcctttaa gtctgtcaat tcgggatata tgattccata
    28381 catctgcaat tatttgatcg taaatagtaa ggtcaccacc gatgcaacta gaaaagatga
    28441 ttactgaagg ctcgaacacc gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt
    28501 gccggattat caacgatgaa gataaaaccg taccgcctgc cgttgatcgc gtcctgccgg
    28561 atatcgccgc ggcgatcgat gttatccacg cccaggtcag cggcggcggt cgtctgattt
    28621 atctcggtgc gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca
    28681 cctacggcgt gaaaccgggt ctggtggttg gtttgattgc tggaggcgaa tatgccattc
    28741 agcacgcggt ggaaggagct gaagatagcc gggaaggtgg tgttaacgat ctgaaaaata
    28801 ttaatttaac ggcacaggat gtggtggttg gcattgctgc cagcggtcgc acgccgtatg
    28861 tgattgccgg actggaatac gcacgccagc tcggctgccg cacagtggga atttcctgta
    28921 atccggggag cgccgtttca accaccgctg agtttgccat tacaccgatt gtaggtgccg
    28981 aagttgttac cggttcttcg cggatgaaag caggtacagc gcagaaactg gtgctcaata
    29041 tgctttccac cgggctgatg attaaatccg gcaaagtgtt cggcaacctg atggtcgatg
    29101 tggtcgccac caacgaaaaa ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg
    29161 gatgtaatgc cgaacaagcg gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg
    29221 ccattgtgat ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac
    29281 acggcggctt tattcgtcag gttttagaca aggaataacc catggccaaa gagatcagca
    29341 gtgaacttct gaacaccatt cttacccgtg tcggcggacc gggaaatatc gccagttgtg
    29401 gtaactgtat gacgcgcctg cgtctgggtg tacatgacag ttcactggtt gatcccaata
    29461 tcaaaacgct ggaaggcgtg aagggcgtca ttttgaccag cgaccaggtg caggtcgttt
    29521 ttgggcctgg taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg ggcgatgcac
    29581 cggtacagga tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa
    29641 cctccggcgt gcaacaattt ctcgccaaat tcgccaccat cttcacgccg ctgatccccg
    29701 gttttattgc cgccggtctg ctgctgggga tagcgacgtt aattgccacg gtgatgcacg
    29761 ttccggcaga tgctcagggc acgctacccg acgcgctgaa ttttatgaag gtgttcagca
    29821 aaggtttgtt caccttccta gtgattctgg tgggctataa cgccgcccag gcatttggcg
    29881 gcacgggcgt caatggcgca attattgccg cactcttttt gctcggttat aaccccgctg
    29941 caaccaccgg ttactacgcc ggttttcacg atttctttgg tctgcccatc gatccgcgcg
    30001 gcaatattat cggcgtgctg attgccgcgt gggcctgcgc ccgcattgaa ggcatggtgc
    30061 gccgctttat gccggacgat ctcgacatgc tgctgacctc gttaatcacc ctgctgatca
    30121 ccgctacgct tgcgtacctg atcattatgc cgctgggtgg ctggttattc gaaggtatgt
    30181 catggctgtt tatgcacctg aacagtaatc cgttcggttg tgcggtttta gccgggctgt
    30241 tcctgatcgc cgtggtattt ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa
    30301 tggacagcca gggattcaac agcttatttc ccatcctttc aatggcaggc gcgggccagg
    30361 tgggcgcggc gctggcactc tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg
    30421 tacgcggggc gattattccc ggcctgctgg gcgttggtga accgctgatt tacggtgtca
    30481 ccctgccccg catgaagccg tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt
    30541 ttatcggatt gatagcctgg tggggtctgc cgatgggctt aaacagcgcc tttgggccgt
    30601 ctggtctggt ggcgctgccg ctgatgactt ccgcacaagg catccttccg gcaatggcgg
    30661 tttatgctgg cgggattctg gtggcatggg tttgcgggtt tattttcacc acgctctttg
    30721 gctgccgtaa cgtcaatctg gactgatatt atgaaacgga caatgctcta tctttctctg
    30781 ctggctgtta gttgtagcgt cagcgccgca aaataccctg ttctgacaga aggtagttga
    30841 tcaagtatat gcggcgttaa agcagtaata aaaaggccag tcagtaatgt tactgcctgg
    30901 cctttaatca atgaatcaga aacgcttaca gcgccatcaa cttgtccagc gacggtgcga
    30961 aataatagcc gccggttacc ggtttggtga aacgcaacat cgcatcacgt ttgccatcgg
    31021 tatcgccaaa catgctcagc agttgctgct caatgttatg cagacgcgcg cagtaggcgc
    31081 agaagtacag accgtgagtg ccactggcgg tgccgtacgg caggctctgg cgaacaatct
    31141 tcagcccttt gccatcttct ttcagatcaa cgcgggtgag gtgagaggtt tccggacgtt
    31201 cgtcgccgtc aatctcttcg ttggcctctt tggtgcgccc gatcatcatc tcctgatcgt
    31261 gaacgctcat ccggttgagc tgcttcaggt tgtgctccca acgctgcaca aacacatagc
    31321 tgccgccgga atccacgccg tctttgataa ccgccacttc gcggcgcgtc tcttcacccg
    31381 ccgggttttc tgtaccgtca acaaagccgc tcaggtcacg ctcttccacc caacggaagc
    31441 cgtggatctc ttctttcact tcaatgcagt caccaaaggc ttccatcgcc gcctgagcga
    31501 cagagaagtt tacgtcgtga cgcagagaaa gaatgtggat caacacatcg aactgggtcg
    31561 tcggcgcaag gcctttaccg tagcccggaa aatctttcag ctcttctgcc ccaacgccgc
    31621 cgctcagagc gcgccaggtg ttgttaccaa aggcaaccac cgcaccaaga tgcgcgtccg
    31681 ggaatttcgc ttcaaaagtt gccagtttgt cggcaaatgt tttactggcc gcacgcaggg
    31741 cgtcaacttc ccctttcacg ttggcttcga tccaaatcgc cgcgcggcaa tgttctggca
    31801 aaatgccact ctgaacctga gacattgttc ctcctgaaaa taagaatgcc acgcaatccg
    31861 tggcattgtt gagcgctatt ttacgtgttt tttcgcgacg gcggtttgtt cagacgcaaa
    31921 ttaacgccgc cagataattt tactgacttt ccagtttttc agggtatcgt ccgaaggcat
    31981 taacccttca ggaccacgcc attccccgct gaactggtag ctaatatgtt gactgccttc
    32041 ggctttacat tcgacggcac gattatcatc accatcagct ttttggcaat tgccaaatgc
    32101 tttgctgtaa aggtcgctaa acggtgtacc gattttaaca ccggtgtcag caggaatatc
    32161 gctatccagc acgtcaatgc gactgatcgt gccttgatcg ccgttaatca ccatcgccac
    32221 gttgtcgcct ttcatcactt caaaaaagcg caccacgttg ccgttcgtgg ttttcattcc
    32281 gctgcgcagg cgataatcgc catcaagcgc atcagcaatg gcttgttctt gcagtggtgt
    32341 ggacgccgtt aattcaccca cgccctgctc gctcactttg gtggacgatc caaaccagtt
    32401 ccacgggtta gcggcagacc agttaactga cgacatcgtg gaacagccgg tcagcatcaa
    32461 cggcatagcg cataacatta aacgcagcga tttcatgtca cttcctttgg ttattcaata
    32521 acgttgcttg gagtgcaaat tcaccaaaaa gtgccgttat tctttttctt cattaaaaca
    32581 agcgcgtaaa cgtcgattgg tcagcagcca gattaacgcc acaatatccg ccactaccag
    32641 cgccagaccg ataccgctaa cggattcacc gttcagccac agccacggtt gccagcaaag
    32701 taaaaccacc tgcgccaaca gcaacagaaa atagagcaca tgccaggtgc gaggaaacga
    32761 agcccgccga ccgctcaaca aaaacgccag caccgccgga ataccgggaa tcagccccag
    32821 ccagaaatta tcgtgatcgg gataaaacag atttagcagc gcagtaccct gctcacgcga
    32881 cgcaccggca atgacaaaca gcacccaggt tcgcgcctga agcaatagca caagccagaa
    32941 gagcaagggt aaacgcaggc gtccgtgcgc atcataatgg acaggatgga actcagtact
    33001 cttcatcttc aatcaaacgc ttacccagac tcagcacgtc ggcgtgttca tatcccaggc
    33061 gttcatacat tccgagcacc atgtcgttat cttccggcac gttgatctga attttcgggc
    33121 agccgcgagc aatcagcttt ttctccagcc ggttaagcaa cgcattggca attccacgcc
    33181 cacgaaactc tggatgcacg ccaagataat aagcagaccc gcgatgcccg tcataaccgc
    33241 ccatcaccgt tccgaccact tcaccgttta cctcagcgac caaaaacaaa ctgacgtcat
    33301 ggttcatctt acgctcgatg tccatttccg gatcgttcca cggacgcagc aagtcgcaac
    33361 gctcccaaag ggtgatgacc tcttcgaaat cttcctggcg aaatacgcgt atctccatgg
    33421 tattcgttac ctttttgcgg gttaaaaggc tgattatggc gtgaacggtc gaattagcca
    33481 atatctgacg aaaatcggtt gaaaaagtgg cataatgggg agttgtcaac tattgaaatg
    33541 aaaagtaaaa caattctcaa cagcaaaccg tcgtaacgga ttacgcgata cgatataaca
    33601 tctggaactt tattattaca actcaggccg tatgagcact tttaaaccac taaaaacact
    33661 cacttcgcgc cgccaggtgc tgaaagccgg tttagctgcc ctgacgttgt caggaatgtc
    33721 gcaagccatt gccaaagacg aacctttaaa aaccagcaac ggacacagca agccgaaagc
    33781 caaaaaatct ggcggcaaac gtgtcgttgt tctcgatcca ggccacggcg gaattgatac
    33841 cggagcgatc ggacgcaacg gttcgaaaga aaaacatgtg gtgctggcga ttgctaaaaa
    33901 cgtccgttcc attttgcgta atcatgggat tgatgcgcgt ttaacgcgtt ctggcgatac
    33961 gtttatccca ctttacgatc gcgttgaaat cgcccataaa catggcgcag atctgtttat
    34021 gtcaattcat gccgatggct ttaccaaccc gaaagctgcc ggtgcttcgg tatttgccct
    34081 ctctaaccgt ggggcaagta gcgcaatggc gaaatacctg tctgaacgcg aaaaccgcgc
    34141 cgatgaagtt gccggtaaaa aggcgactga caaggatcac ctattgcagc aagtactgtt
    34201 tgatctggtg caaaccgata ctattaaaaa cagcctgacg cttggctcgc atattctgaa
    34261 aaagattaag ccggtgcata aactgcacag ccgcaacacc gaacaagccg cgtttgtggt
    34321 gttgaaatcg ccgtcggttc cttcggtgct ggtggaaacc tcgtttatca ccaacccgga
    34381 agaagagcgg ctgttaggca cggcggcgtt tcgtcagaaa atcgccacag cgattgctga
    34441 aggcgtgatc agttatttcc actggttcga caaccagaaa gcacattcga aaaagcgata
    34501 agttatgaaa cccgacgcac accaggttaa acagtttctg ctcaaccttc aggatacgat
    34561 ttgtcagcag ctgaccgccg tcgatggcgc agaatttgtc gaagatagtt ggcagcgcga
    34621 agctggcggc ggcgggcgca gtcgggtgtt gcgtaatggt ggtgttttcg aacaggcggg
    34681 cgtcaacttt tctcacgtcc acggtgaggc gatgcctgct tccgccaccg ctcatcgccc
    34741 ggaacttgcc gggcgcagtt tcgaggcgat gggcgtttca ctggtagtgc atccgcataa
    34801 cccgtatgtt cccaccagcc acgcgaatgt gcgttttttt attgccgaaa aaccgggtgc
    34861 agagcccgtt tggtggtttg gcggcggctt cgatttaacc cctttctatg gttttgaaga
    34921 agacgccatt cactggcacc gcaccgcccg tgacctgtgc cagccatttg gtgaagacgt
    34981 ttatccccgt tacaaaaagt ggtgcgacga ttacttctac ctcaaacatc gcaacgaaca
    35041 gcgcggcatt ggcgggctgt tctttgatga tctgaacacg ccagatttcg accactgttt
    35101 tgcctttatg caggcggtag gcaaaggcta caccgacgct tatttaccaa ttgtagagcg
    35161 acgtaaagcg atggcctacg gcgagcgcga gcgcaatttt cagctctacc gtcgcggtcg
    35221 ttatgtcgag ttcaatctgg tctgggatcg cggcacgctg tttggcctgc aaactggcgg
    35281 gcgcaccgag tcgatcctga tgtcaatgcc gccactggta cgctgggagt atgattatca
    35341 gccaaaagat ggcagcccag aagcggcgtt aagtgagttt attaaggtca gggattgggt
    35401 gtaactccct cacccccatt cccgcatccg ctgatgcaac gtcaacgacg gcttctcggc
    35461 aaacagctgc tggtaatccg tggcaaattg ccccagatgc cagaatcccc actgcatggc
    35521 ggcgtctttt accgttgtgc tttgcgacca cgggcttatc agttcgcggc gtacgacgtt
    35581 caggcgaatg cgtttcagcc acgcgtttgg gccaatgcct aaaatagcgt gaaacgcgtt
    35641 ttgtagcgtg cggcgactga catgcagttg attacacaaa tccagcaccg tcaccggttc
    35701 ggacatgttt tccagcacat attcacgggc gcgggaaagc aaccgacggt aactctgatg
    35761 actgatgctt tccgccgtca ccattggttg cgcttcttcc agcatcgccc ccatcgccat
    35821 tagcaaatta tcccccagca cttttcgcac tgcaggctga tggagatttt ccgggttctc
    35881 gcaaaacgtc gccagcgcct gttggacaaa gccccacagc gcggctttat gctgctcttt
    35941 cacttccagc gccgactggc tacgcagcat atgtaatacc cgatccggat tgtgcaaaaa
    36001 gttagcctga caggtgatga catcttcaga cagcactacg cccaggatcg tataatcgtc
    36061 cggcgtactt aattcaaact cggttccacc ggggcgcgtc gctatttctg cgcttcccag
    36121 acattgcgaa ccgataaatc cctgctcacc gcgcgtcgcc ggaatgccaa accagaacga
    36181 gttcggccag accaggcacg actgacgcag cgccagaccg gtgtattcac gaaaaacctg
    36241 aatatcatcg agtagaattt ccgtaaattc accatgaaac ttgcccggat gcagctgatc
    36301 gtaaatctgc tgctaggcgg taatcgtcaa agcatgttca tagacatccg ttgtctgtcg
    36361 ttgatgaaca ttatccactt cgaccttcgg cgtgagcttc aggttttcgg gtaagggttc
    36421 atgataaaga tggtgcaaat tggctgtacg ggtctttttc atgatgttaa tgccgggtgt
    36481 tgtaggacac ccggcacctc cgacaggtta atggggcttg agacgataac gactactgcg
    36541 tttacgtaac gtcccggcag aaaagagctg ttcgagcgca tttctggctt tttccagcgg
    36601 ccagccaaag tgggcggcca cttctcctgc cgtcattccc tgacatactg atgttaacag
    36661 cgccaacaac tcgtcagcag attccgcgac gataactggc gcgtcgggtt cctttacggg
    36721 ttgcttcggc tggcggttaa agccagtaac cagccactgg atgtcatcgt ctggccgacc
    36781 gatctcctta cggctgatga cacgcccggt acgcatcgcg gcagcacaac ctgcgtccag
    36841 cgccgcacga cacgccgcca gatcgccttc caccaccagc gttaagcgac cagggtcaag
    36901 cacttcgtga ctgagcagac gaacgttagc tgctttgagc atggcatccg ctgcatctat
    36961 cgcggcgacc atgccgtcca cttccagcaa tcccagtgca ttgatcatcg gcaacctccg
    37021 ttacgcacgc tggattggat tacgcgcgat ttccagcact gcatcggtaa aggcgttaca
    37081 ggctgcttta cacgcggcct ggctaccggt taaaaatgct gccgagtagt tggtttcaga
    37141 cggtggcggg acgtaggtca ccagctgtac gtcggcagat ttcaacgcgg catcaatgcc
    37201 gtaggtcgct tccagcggcg gtgccaccag atacgccatc ggatcgccaa gcgtgatccc
    37261 ggcggttgat gaaagataag aaccggtacg cgaaaccaca tgcgccagga aggcggtgtt
    37321 ttctgcgtca ttggcccact ggaaagctgc gccattttca atatgtgcaa ccatcgcatc
    37381 cagaccggca cgcacttccg ccgggtttgg cccaccaagc ataatcagca cttcaccggc
    37441 agtcggtgac gggccgtgtg ccgctcccgc atacagcgag cgaccatata ccacttcgac
    37501 catcgcttgt ttggtcgctt cgtcagccgc aatgtaggtt acgtcgtcag aatcggcaga
    37561 aatcagcccg aggctacgaa tatgcggcgg caattttagt tcacgcgcaa attcagcgtt
    37621 aacggaggca atcacccgca tggcggttac cgacggtcga atcaaatcta aagctggcat
    37681 gatgcctcct tatcgggtca tgttgatgcc ggatgctttc tgctccagca tacgtttcgc
    37741 caaatccaca atgacggcac agcagcacga aagagaagca aaagtcagct acctggtaca
    37801 ctaggcagcg ggtcagcaaa ggggggcaaa tgcccctttt ttcatgtcag ataatcagat
    37861 cattacctca ataaactaat aatcacatcg cggttacaac cagtttgata gtgatagctc
    37921 aaacccagac gtggcgctgg ctgcaagcaa aataagccgc aaaaatgcga ccccatgttt
    37981 actagccgca aaaatgcggt cctatttgcc agaacctttt attattcaat tggttagtgc
    38041 aagaaaaagc gtgattgctg ttcaagaagt atccagaaag gtcatgattt tgacgctgaa
    38101 aaaaaatagg tgggcatttt tgcccactca ctagccgcaa atttgcccac tcactagccg
    38161 catttttgcg gcccaaagta ataaatacag agaaataata aatacagatc agcgccaaga
    38221 tttttcgtct tttcggcgga atcatgagac tagtgcttac gtcctgataa cctccagagc
    38281 agaccttaac ttctgtgcag caggactacg ctcattgccg ctcggctagg tcaaatcctc
    38341 acgataaagc tgttcggatt gtgaccataa tgttaaagga ctctttaaat cattccttga
    38401 cgaattaact agctggctta agcggaatag catgcaggat aaaaacaaaa ccagcttagc
    38461 agtggggaga gcttcgcttc gctcgtctcc tggggccaat gctcaatgaa acgacgtaag
    38521 gcacggagcg ctcaccatag gtagacacat aaacatctga gagcgcacct tctatcgcgc
    38581 tacgcgctca ataaaaacca acgacaccgc cacactgcaa gaccaatggt ttttatcgcg
    38641 tatacagacc agacaggaaa cagcagcaaa gcagcgtata gccaggtgca gacagcacac
    38701 tcacacgacg cacagcaggc ttaagtgatc actcaggaaa ctttatcccc gccgccgatc
    38761 accgcgaact atcatgaccg cgccaaattc gaaaggaatg gcccatagaa cgcgatgaac
    38821 acacataagc gctacactta cttaacgtcg ccacagagcg attgagagcg cttaaaatcg
    38881 atgcgatttc attaaatttt tctcactcgc ctgaatggtg gcgatcagga ctgatcgcaa
    38941 cattgccccg cctcgatatt gagcgcataa tagatacata cattaagcgg aaaagagata
    39001 agtttattgt gaaaatcagg gggttacata tcatgcgcat atcagaacaa caacggcaga
    39061 tcctcgacta tctggcaaaa aacgaatcaa tcaccgcacg acaagccgca gatctcgttt
    39121 atggtggcaa tgttacccgc gtccaggttg acgccgcccg ccgctcactc atgacgatgg
    39181 ttagtaatgg gctaatcagg aaacaaggcc gcgctttcgt agctgaaaga catatcaatc
    39241 acgcttatag ccaggacgtt gaagaattgc gccgccagat cctttgggta ttcagcaaac
    39301 agggtcagga gtatttcctt tctgaacacc caaatttcgc cgccaaagag ttaacagtaa
    39361 gcggattgtc gtgcgcactg gattatcttg tcggtgaagg ttgcgttact tacaagcagc
    39421 tatggcacgc cctgaatacg cttgtagaag atggtttact cattgccacg cgtgagaaca
    39481 tcgaatactg gataatgccg cgttcaggtc gccgcaatca tgcacggtat atttcaggca
    39541 acagatacag aacgccggac tttaatccga cacaggaaga tcgggaccgt gccgaaatga
    39601 tgtgcaggga acaggagaaa agaattatgg ataagttatt ttcgatgcag tgaacacaag
    39661 cgggcgcaat gcccgttttt attgccttat tccacgcctg acgaatcaat ttgatcacac
    39721 ctgagaaaat gcatagccag aagttaaggc cgttatattg cgattgggag cgttttaaac
    39781 ggtatcaatt taattaattc tgatgtggtg atttgcaggc aaaaaaaacc cgccggagaa
    39841 gcgggaaata gagacatatc gttgtagtta ttaacagatc actttgaggt cgagggcggc
    39901 taaccctcgc taatattatc ccgctattac cttgcgggtg caaataatct tttattgctt
    39961 tgatgattta ataaatagca ctttttgcta acaaaaaccg ctgtcaatat atggccaaaa
    40021 atgtcaacag aaaaaataag gtttataata ttaagtatca catgggtaat gggttcaggg
    40081 aatcggaatc caccgcctca cggaaattca aaatttgctt cttctttcct ccgtgggcgg
    40141 cttctttttc gtatcgaggt atttatgaaa gcaataacat tagaacagtt cgcagcaatg
    40201 gcgaatgtat cgaccagaac gctgaggcgc tggctaaaaa aaccatcaac cggagtccct
    40261 ttgccattta ccccacgcgg tactcgccaa ttattctggg agcacgaatg caaagaatgg
    40321 ttgtacagaa cagaacgata ataatcacgc atcacatttt gaggtaaaca tcatgaccaa
    40381 tatcgaaaga ttagaacgct gtgccgatat tatgcgacgc agatggattt atgaccctga
    40441 agcgggttta ttgtttagcc gtgaaacaaa agacgttatc aaaggcagca tgaccaataa
    40501 agggcatttg gttgtaacgg tacatgaagg taaattcaaa gtgacactca cctatcagaa
    40561 agcctgctat gtatacacat acggcgctta tgacgagaca ttatatgaag tgcaccacgt
    40621 taatttgaac aaacaggata accgcatcaa gaatattcgc ctgatgccga aagaaaagca
    40681 ccgccggata cataaaaacg tccgcaaact tattcggctg ggccacacac aatggcttca
    40741 accgcaatta attgaacaat cactataaac acaggagtaa ataccatgca ttcattagac
    40801 agttattttc agcgcacgac agcccctaaa tcagccgccc aggaacgccg tgaggaattc
    40861 catgagaaag tgatgcgttc tgctgattat attgcggaca agttcgtgga aacggttcgt
    40921 ccacttgttg atgaagtagc tgataaactt cagtctgaaa tgccggaaga tatggaaggc
    40981 actgcaaagc ggcgactcat ttgcgaatta agtcgtcgtt ttggcgtgtc aataagcgct
    41041 tttaaataat cgaaaaaagg ggggaaagat aatggacgca tttgaggtac tggcaaataa
    41101 aggcgctgaa ctggtagcac agcgtgataa aaccgccaac gaaggggaac gcgcactgct
    41161 aaataaacag attaaagcga tccgtatggc ccagtttaaa ttaatcagta atgaagttat
    41221 cgaaacaatt aaaccgcaga tcccacaaat cgttgctgac gctattaaag ccgccggact
    41281 ggagaaacgc attgccgggc taaaaacgaa cgcagagaaa atggagatgt acaaacagag
    41341 cggtgctccg aatcccgatg agtacttctc accggatgtt gagtttatgg cgcaggttga
    41401 ggaacgtctg ggtagctgtc tcaccgagga acagcgtcgc tattttgatg gagtggatag
    41461 ttcggcaggt attgacctga actcgtattt cggtcgtgaa attgagcatt ttgatgccgc
    41521 cccgtctatg ccggagccag aaccagaaat ggccaccgat cctgaagctg agcaacggaa
    41581 acgcgtattc aatgcgatag ggattcggta ctgatcggat aaaagaaagc cgggaattac
    41641 cccggctgac ttaacaatgt gaccaatgaa caaatcggac aagacaaaca ggataagcag
    41701 tctcgaaaca atgttcggga actatattag cacaagtccg gagaaaatca tcatgttggc
    41761 actacgcatc atgcagggca tcgctaagac cctcgcagaa catgtcttag acctgaaaca
    41821 ttcgccactg agcaaacagg ccatgaagcg ccaaacgctt cgtctgtggg ctgaatattc
    41881 gcttggaact atcaataaaa tcatcgacat gaaatcgggg ccgagcaacc agagcgcgga
    41941 ggaaatggag ttcatcaggc gtttaattct cattcgcaga gacattcata gccagttaca
    42001 cagcgtggga atcgatatta atgatgggac gggcgattaa ttttcggaca agtactatgc
    42061 gcgcgcgagg agctgaacat gaagcaagat attgcagaca ggctggagat actcgaaggc
    42121 cagcgggcag aagcgaaaca actacgtaag caggccagaa gagcgcacag gaacaacgaa
    42181 gcagagttac taaccaaata catcagtttc accaattact gcatctacga gtgttacaag
    42241 gaagatgcag aggactggct ggattcattg ccagaacagt actgagcaga ataacaccac
    42301 gtcatgttgt tctgaggtga gcatatcacc tctgttcagg tggccaaatt tagtgtgcca
    42361 cttcagcatc accgttgaaa tcacaataac gatttttttg taaatatgct ctcac
//
LOCUS       DAZXUK010000008        39711 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZXUK010000008 DAZXUK010000000
VERSION     DAZXUK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39711
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.008"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            378..1595
                     /gene="alaA"
                     /locus_tag="ACH3L8_000358"
     CDS             378..1595
                     /gene="alaA"
                     /locus_tag="ACH3L8_000358"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFT4083633.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1679..2278
                     /gene="yfbR"
                     /locus_tag="ACH3L8_000359"
     CDS             1679..2278
                     /gene="yfbR"
                     /locus_tag="ACH3L8_000359"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFT4083634.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLMKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2337..4169)
                     /locus_tag="ACH3L8_000360"
     CDS             complement(2337..4169)
                     /locus_tag="ACH3L8_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFT4083635.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILTVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4256..4906)
                     /gene="hxpA"
                     /locus_tag="ACH3L8_000361"
     CDS             complement(4256..4906)
                     /gene="hxpA"
                     /locus_tag="ACH3L8_000361"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFT4083636.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEADLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4917..5411)
                     /locus_tag="ACH3L8_000362"
     CDS             complement(4917..5411)
                     /locus_tag="ACH3L8_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFT4083637.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5494..5949)
                     /gene="yfbV"
                     /locus_tag="ACH3L8_000363"
     CDS             complement(5494..5949)
                     /gene="yfbV"
                     /locus_tag="ACH3L8_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFT4083638.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6287..7489
                     /gene="ackA"
                     /locus_tag="ACH3L8_000364"
     CDS             6287..7489
                     /gene="ackA"
                     /locus_tag="ACH3L8_000364"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFT4083639.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7564..9708
                     /gene="pta"
                     /locus_tag="ACH3L8_000365"
     CDS             7564..9708
                     /gene="pta"
                     /locus_tag="ACH3L8_000365"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFT4083640.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9898..11418
                     /gene="yfcC"
                     /locus_tag="ACH3L8_000366"
     CDS             9898..11418
                     /gene="yfcC"
                     /locus_tag="ACH3L8_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFT4083641.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11451..11993)
                     /gene="yfcD"
                     /locus_tag="ACH3L8_000367"
     CDS             complement(11451..11993)
                     /gene="yfcD"
                     /locus_tag="ACH3L8_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFT4083642.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12051..12602)
                     /gene="yfcE"
                     /locus_tag="ACH3L8_000368"
     CDS             complement(12051..12602)
                     /gene="yfcE"
                     /locus_tag="ACH3L8_000368"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFT4083643.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12658..13302)
                     /gene="yfcF"
                     /locus_tag="ACH3L8_000369"
     CDS             complement(12658..13302)
                     /gene="yfcF"
                     /locus_tag="ACH3L8_000369"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFT4083644.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13438..14085
                     /gene="yfcG"
                     /locus_tag="ACH3L8_000370"
     CDS             13438..14085
                     /gene="yfcG"
                     /locus_tag="ACH3L8_000370"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFT4083645.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14142..14504
                     /gene="folX"
                     /locus_tag="ACH3L8_000371"
     CDS             14142..14504
                     /gene="folX"
                     /locus_tag="ACH3L8_000371"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFT4083646.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14525..15418
                     /locus_tag="ACH3L8_000372"
     CDS             14525..15418
                     /locus_tag="ACH3L8_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFT4083647.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15466..16356)
                     /gene="rpnB"
                     /locus_tag="ACH3L8_000373"
     CDS             complement(15466..16356)
                     /gene="rpnB"
                     /locus_tag="ACH3L8_000373"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFT4083648.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16553..17326)
                     /gene="hisP"
                     /locus_tag="ACH3L8_000374"
     CDS             complement(16553..17326)
                     /gene="hisP"
                     /locus_tag="ACH3L8_000374"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFT4083649.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17334..18050)
                     /locus_tag="ACH3L8_000375"
     CDS             complement(17334..18050)
                     /locus_tag="ACH3L8_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4083650.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18047..18733)
                     /gene="hisQ"
                     /locus_tag="ACH3L8_000376"
     CDS             complement(18047..18733)
                     /gene="hisQ"
                     /locus_tag="ACH3L8_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFT4083651.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18823..19605)
                     /gene="hisJ"
                     /locus_tag="ACH3L8_000377"
     CDS             complement(18823..19605)
                     /gene="hisJ"
                     /locus_tag="ACH3L8_000377"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFT4083652.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19826..20608)
                     /gene="argT"
                     /locus_tag="ACH3L8_000378"
     CDS             complement(19826..20608)
                     /gene="argT"
                     /locus_tag="ACH3L8_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFT4083653.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20874..21443)
                     /gene="ubiX"
                     /locus_tag="ACH3L8_000379"
     CDS             complement(20874..21443)
                     /gene="ubiX"
                     /locus_tag="ACH3L8_000379"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFT4083654.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21538..23055)
                     /gene="purF"
                     /locus_tag="ACH3L8_000380"
     CDS             complement(21538..23055)
                     /gene="purF"
                     /locus_tag="ACH3L8_000380"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFT4083655.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23092..23580)
                     /gene="cvpA"
                     /locus_tag="ACH3L8_000381"
     CDS             complement(23092..23580)
                     /gene="cvpA"
                     /locus_tag="ACH3L8_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFT4083656.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23839..24501)
                     /gene="dedD"
                     /locus_tag="ACH3L8_000382"
     CDS             complement(23839..24501)
                     /gene="dedD"
                     /locus_tag="ACH3L8_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFT4083657.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24491..25759)
                     /gene="folC"
                     /locus_tag="ACH3L8_000383"
     CDS             complement(24491..25759)
                     /gene="folC"
                     /locus_tag="ACH3L8_000383"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFT4083658.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25829..26743)
                     /gene="accD"
                     /locus_tag="ACH3L8_000384"
     CDS             complement(25829..26743)
                     /gene="accD"
                     /locus_tag="ACH3L8_000384"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFT4083659.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26899..27558)
                     /locus_tag="ACH3L8_000385"
     CDS             complement(26899..27558)
                     /locus_tag="ACH3L8_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFT4083660.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27641..28453)
                     /gene="truA"
                     /locus_tag="ACH3L8_000386"
     CDS             complement(27641..28453)
                     /gene="truA"
                     /locus_tag="ACH3L8_000386"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFT4083661.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28453..29466)
                     /locus_tag="ACH3L8_000387"
     CDS             complement(28453..29466)
                     /locus_tag="ACH3L8_000387"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFT4083662.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29532..30668)
                     /gene="pdxB"
                     /locus_tag="ACH3L8_000388"
     CDS             complement(29532..30668)
                     /gene="pdxB"
                     /locus_tag="ACH3L8_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFT4083663.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30767..31762
                     /gene="flk"
                     /locus_tag="ACH3L8_000389"
     CDS             30767..31762
                     /gene="flk"
                     /locus_tag="ACH3L8_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFT4083664.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31759..32937)
                     /locus_tag="ACH3L8_000390"
     CDS             complement(31759..32937)
                     /locus_tag="ACH3L8_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4083665.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33192..34433)
                     /gene="fabB"
                     /locus_tag="ACH3L8_000391"
     CDS             complement(33192..34433)
                     /gene="fabB"
                     /locus_tag="ACH3L8_000391"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFT4083666.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            34592..36598
                     /gene="mnmC"
                     /locus_tag="ACH3L8_000392"
     CDS             34592..36598
                     /gene="mnmC"
                     /locus_tag="ACH3L8_000392"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFT4083667.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36719..36997)
                     /locus_tag="ACH3L8_000393"
     CDS             complement(36719..36997)
                     /locus_tag="ACH3L8_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFT4083668.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(37031..37579)
                     /gene="epmC"
                     /locus_tag="ACH3L8_000394"
     CDS             complement(37031..37579)
                     /gene="epmC"
                     /locus_tag="ACH3L8_000394"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFT4083669.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37579..38388)
                     /locus_tag="ACH3L8_000395"
     CDS             complement(37579..38388)
                     /locus_tag="ACH3L8_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFT4083670.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38388..39212)
                     /gene="mepA"
                     /locus_tag="ACH3L8_000396"
     CDS             complement(38388..39212)
                     /gene="mepA"
                     /locus_tag="ACH3L8_000396"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFT4083671.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39216..>39711)
                     /locus_tag="ACH3L8_000397"
     CDS             complement(39216..>39711)
                     /locus_tag="ACH3L8_000397"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFT4083672.1"
                     /translation="KEGDSIGAKVTVVASGVPAGLGEPVFDRLDADIAHALMSINAVK
                     GVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGIFGGISSGQQIIAHMALKPTSSIT
                     VPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEAMLAIVLMDHLLRQRAQNADVKTD
                     IPRW"
BASE COUNT         9816 a        10817 c         9867 g         9211 t
ORIGIN      
        1 ttaacaatag tttcacacct tacccccatt gcgacacctc ggaaatcatc acgctgttaa
       61 actctccgta atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca
      121 ttttcacaca attatcttac agataaaaaa accagacttg caattaagaa ttagaacaat
      181 tcactatata acattgcatg taaagcatat aagcctcatt atttgtcatt attaagttat
      241 taacagcaca atcgagcctt cccctctggc aaaatcttat tctgcagacc ttcaaaacac
      301 cgtcctgggg gagtacattg ttctaagctg acttccacgg cagggagtgg cgataacagc
      361 aaaaaaggtc aagattcatg tcccccattg aaaaatccag caaattagag aatgtctgtt
      421 atgacatccg tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg
      481 tactgaaact gaacatcggc aacccagccc cattcggttt tgacgcgcca gatgaaatcc
      541 tcgttgacgt gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc aaagggcttt
      601 actccgcgcg taaagccatc atgcagcact accaggctcg tggcatgcgt gatgttaccg
      661 tggaagatat ttacatcggc aatggtgtat cggagcttat cgttcaggca atgcaggcat
      721 tgctgaacag cggggacgaa atgttggttc ctgcaccaga ttacccactc tggaccgcgg
      781 cggtttcgct ttccagcggt aaagcggtgc attatctttg cgatgaatcc tctgactggt
      841 tcccggacct cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg attgttatta
      901 tcaacccaaa taacccaacc ggcgcggtat attccaaaga gcttttaatg gagattgtgg
      961 agattgcacg tcagcataat ctcattatct tcgccgatga aatttatgac aaaattctct
     1021 acgacgatgc tgagcatcac tcaattgcgc cgctggcacc tgacctgctg accattacct
     1081 ttaacggact gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg atggtgttga
     1141 acgggccgaa aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc
     1201 gcctgtgtgc taacgttcct gcgcaacacg ccattcagac cgcgctgggt ggttatcaga
     1261 gcatcagtga atttattacc cctggcggtc gtctttatga gcagcgtaac cgcgcgtggg
     1321 aactgatcaa cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg ctgtatatgt
     1381 tcccgaaaat cgacgccaaa cgctttaaca ttcatgacga tcagaaaatg gtgctggatt
     1441 tcctgttgca ggaaaaagtt ctgttggtgc aagggacggc attcaactgg ccgtggccgg
     1501 atcacttccg cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct ttgagcaagt
     1561 tcgcgcgttt cctttctggt tatcatcagc tgtaatctta atttcactgc cggagattgc
     1621 atccggcagt gttatcccgc cacaatgacc tgatgatgtc atcatacgta aggtcactat
     1681 gaaacagagc catttttttg cccatctctc ccgcctgaaa ctcattaacc gctggccgct
     1741 aatgcgcaac gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag cgatggtcgc
     1801 ccatgcgctg gcagctatca aaaatcggaa atttggcggt aatgtcaacg ccgaacgtat
     1861 cgctttactg gcgatgtacc acgatgcctc agaagtgctc accggcgatc tccctactcc
     1921 ggtgaaatac ttcaatgcgc aaatcgctca ggagtacaag gctattgaaa aaatcgctca
     1981 gcagaaactg gtcgatatgg ttccggaaga actgcgggat atctttgcgc cgttaattga
     2041 cgagcatgca tatagcgatg aagaaaaatc gctgatgaaa caggcagatg cactgtgtgc
     2101 atatctgaaa tgtctggaag aactcgcggc cggaaataat gaattcttgc tggcaaaaac
     2161 gcgactggaa gcgacgcttg aagcgcgtcg cagccaggag atggactact tcatggaagt
     2221 atttgttccc agcttccatc tttcgctcga tgagattagc caggattcac cgctgtaagc
     2281 agccggagtc tgcgtcgcat cagtcaataa gcgccggatg cgacatcagg ctcttgtcaa
     2341 aacggaaaca gcatcgggat catcaccaca caaaccgcca tcacgataat ggtgaacggt
     2401 acccccaact tcacaaagtc actgaagctg taatttcccg gacctaaaac cagggtgtta
     2461 acaggtgaag acaccggtgt cataaaggcg gcggatgctg ccatcgccac gaccatcgca
     2521 aatggataag gcgacactcc catcgttttg gcagcagcca gcgcaatcgg agccatcaac
     2581 accgccgtcg cggtattaga gataaacagc ccaataaccg ccgacaagac aaacaaacac
     2641 cccagcatca tatgtggccc ataaccgccg ccaatatcca tcagcccttt caccgccagc
     2701 gcgacacctc ccgttttctg taatgccaca gcaaacggca tcatcccaac ggtcaaaata
     2761 atgctcggcc agtgaatgga tttataggcg ctttcagcat ctatacagcg gaatttcccc
     2821 atcagcaggc aggcgatgat agcggcaaca ggattaggaa tttcatttgt cagcattaac
     2881 gccaccatta gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc gggcgatgct
     2941 tcactcacct cttccggtaa gttcagcgct acgaagtcgc ggccctgttt ggccagcata
     3001 ccgatcagtt tccagttacc cacaaccagg atgatatcgc ccagcagcag aggctcatcc
     3061 gccagcgaac cttccagcgc cacgccattg cgcttgagcc ccaccacatt cagtccgtag
     3121 cgggtacgaa aaccaatttc gcgcaccgat ttaccaatca gttctgactc aggaattaat
     3181 gaaatctctg ccatgcccac atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc
     3241 atcggctcca gcaattgctc actacaaaat tgccggagat cgacatcagc cgcagacata
     3301 tcaataagca aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg
     3361 cgacgaaaac gccgccagcg ttcaacaccg atgacgttag cgccataacg ctcacgtaat
     3421 ttgagatcat ccagccgttg acctatcatt ggcgatccgg ggcgaatagc cagacgtcgc
     3481 gcacgcccgg tcagtcgata ttcacggata agatcgcgaa aggttcgacg cgtccagcct
     3541 tcgcgctgcg gggtctgggt atcccctttc agcatgaaac gcatcactaa catatacaaa
     3601 atacccagca ccagcacgac caggccaata ggtgttacgc taaagaaact gaagccgtga
     3661 tagccttcac gcagcaattc actgttgact accaggttcg gcggcgtcgc caccagcgtc
     3721 atcatgccgc taatcagccc ggcaaaactt aacggcatca tcagacgtga cggcgaagtt
     3781 tgcatacgca tggcaacgct taacaccacg ggaataaaga tagcgacaac gctggttgaa
     3841 ctcataaacg cgccaagccc cgcgacggtc agcatcaaca aaaccaacat tttgatttca
     3901 ctgttgcccg ccactttgac cagccatgtt cccattacgg tggcaacacc ggtacggacc
     3961 aaaccatcgc caataataaa caaggcggca atcaggacaa cgttaggatc agaaaagccg
     4021 gaaaatactt cggggaccgt cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa
     4081 gcgaccgcat ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac ggccagtaat
     4141 gaaagaaccc aaatcaattc accgttcaca acttatcctt gttaattgag ggggatgact
     4201 tgattctgcc ataaaaaagc cccgacgaga cggggctaaa tcatgatcag gtgtttcact
     4261 gaataataac atcgccattt ggctgtttgg tcacagtaat ttgctccaga ctgtggagga
     4321 ccaaatcggc ctcattcagg cgcggggtat ctgccggagc gttaaccgca atgacatggc
     4381 aacccgccgc caggccagaa agcacgccag cgggagcatc ttccaccacc acacactcct
     4441 gcggcgcaag ccccagcagc tgcgcaccta acagatacgc atcaggttct ggttttccgc
     4501 gcttcactcg ctcagcggtt acaaacacct ctggtgcggg aagccccgct attttatggc
     4561 gcgctcgcgc taccggcatg gagccagaag tcacaatggc ccacggaata cctgctttat
     4621 tcaaatgatt gagtaaggcg attgccccag gaagcgcggt aataccttcg gtttccgtgg
     4681 cctcgatttg ctccagacgc gtaaactcgg cggcaatatc agcctcggat ttgcccgcca
     4741 taaaatggcg caaagaggtg atcgcctgtt taccgtgaat gaaagccagc acctcttccg
     4801 gcgctaaccc atgacgtctg gcccagttgc tccacgcccg ttctaccgca ggcagggaat
     4861 ccaccagcgt tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg gcctcctcag
     4921 gcgttgataa tttgattaat ttcgttggcg ctcaaatggt actgacgcgg gcaggcatgc
     4981 cacacattaa gcatgcgctg atatttttcc cacattggcg tctgggcgtt aaaaccatga
     5041 gtcccagcgt caaaatgggt atagcgccct tccacattaa ccataaagcg gacataaccg
     5101 aggtaacgtg cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg ttcatcgatg
     5161 gattgctggt cctgcaaatt agaccaggaa acatacaacg catgatacat atccataatg
     5221 tcgatgatag tgcggcaggt ttcttctttc agctcgccaa actcgcgatc caattcacgc
     5281 atctgtaatc cgtaaccacg ctcaataatt gtttgcaggc gacggtaacg ttcagcattt
     5341 gccggatcga gcatagtcat catcttgtac tgattagaca aaataagacg ttgcgcgttg
     5401 gtcatttcca ttgttgactc ctgtatcact ctactacggt gaaaaaaaag aaggctgagt
     5461 atgccttctt ttatatgcgt aatcaggggt caattacaaa tcatcaagga aagttttatc
     5521 cagttgtttg aaggcgcgct taagcgtgtc agctaatgcc tggtaatcag gcttgccttc
     5581 aacgggtgcc aacacctgtc cagactcctg caatttaccg cgaacttcat aaaaccagtt
     5641 aaggattgca gggggtaatg gcgttacaga acgcttgccc agccaccaca atccctgcat
     5701 gggtaaactt aaggcgaaca gtgcagtggc aactgccggc ccaagcagac cgcccagggc
     5761 aatctgccag cagagagtaa atacggcgat cggcggcata aaacggatcg cataacgcgt
     5821 catcttgata acgcgatttt cgacaaagac cggagcaagg cgtttttcca gtggccacgt
     5881 ctttgagtaa tgctgtccgc ggcgaaacaa gctaaaaaaa ttaacagaac gattatccgg
     5941 cgttgacatg cttcacctca acttcacata taaagattca aaaatttgtg caaattcaca
     6001 actcagcggg acaacgttca aaacattttg tcttccatac ccactatcag gtatccttta
     6061 gcagcctgaa ggcctaagta gtacatattc attgagtcgt caaattcata tacattatgc
     6121 cattggctga aaattacgca aaatggcata gactcaagat atttcttcca tcatgcaaaa
     6181 aaaatttgca gtgcatgatg ttaatcataa atgtcggtgt catcatgcgc tacgctctat
     6241 ggctccctga cgttttttta gccacgtatc aattataggt acttccatgt cgagtaagtt
     6301 agtactggtt ctgaactgcg gtagttcttc actgaaattt gccatcatcg atgcagtaaa
     6361 tggtgaagag tacctttctg gtttagccga atgtttccac ctgcccgaag cacgtatcaa
     6421 atggaaaatg gacggcaata aacaggaagc ggctttaggt gcaggcgccg ctcacagcga
     6481 agcgctcaac tttatcgtta atactattct ggcacaaaaa ccagaactgt ctgcgcagct
     6541 gactgctatc ggtcaccgta tcgtacacgg cggcgaaaag tataccagct ccgtagtgat
     6601 cgatgagtct gttattcagg gtatcaaaga tgcagcttct tttgcaccgc tgcacaaccc
     6661 ggctcacctg atcggtatcg aagaagctct gaaatctttc ccacagctga aagacaaaaa
     6721 cgttgctgta tttgacaccg cgttccacca gactatgccg gaagagtctt acctctacgc
     6781 cctgccgtac aacctgtaca aagagcacgg catccgtcgt tacggcgcgc acggcaccag
     6841 ccacttctat gtaacccagg aagcggcaaa aatgctgaac aaaccggtag aagaactgaa
     6901 catcatcacc tgccacctgg gcaacggtgg ttccgtttct gctatccgca acggtaaatg
     6961 cgttgacacc tctatgggtc tgaccccgct ggaaggtctg gtcatgggta cccgttctgg
     7021 tgatatcgat ccggcgatca tcttccacct gcacgacacc ctgggcatga gcgttgacgc
     7081 aatcaacaaa ctgctgacca aagagtctgg cctgctgggt ctgaccgaag tgaccagcga
     7141 ctgccgctat gttgaagaca actacgcgac gaaagaagac gcgaagcgcg caatggacgt
     7201 ttactgccac cgcctggcga aatacatcgg tgcctacact gcgctgatgg atggtcgtct
     7261 ggacgctgtt gcattcactg gtggtatcgg tgaaaatgcc gcgatggttc gtgaactgtc
     7321 tctgggcaaa ctgggcgtgc tgggctttga agttgatcat gaacgcaacc tggctgcacg
     7381 tttcggcaaa tctggtttca tcaacaaaga aggtacccgt cctgcggtgg ttatcccaac
     7441 caacgaagaa ctggttatcg cgcaagacgc gagccgcctg actgcctgat ttcacaccgc
     7501 cagctcagct ggcggtgctg ttttgtaacc cgccaaatcg gcggtaacga aagaggataa
     7561 accgtgtccc gtattattat gctgatccct accggaacca gcgtcggtct gaccagcgtc
     7621 agccttggcg tgatccgtgc aatggaacgc aaaggcgttc gtctgagcgt tttcaaacct
     7681 atcgctcagc cgcgtaccgg tggcgatgcg cccgatcaga ctacgactat cgtgcgtgcg
     7741 aactcttcca ccacgacggc cgctgaaccg ctgaaaatga gctacgttga aggtctgctt
     7801 tccagcaatc agaaagatgt gctgatggaa gagatcgtcg caaactacca cgctaacacc
     7861 aaagacgctg aagtcgttct ggttgaaggt ctggtcccga cacgtaagca ccagtttgcc
     7921 cagtctctga actacgaaat cgctaaaacg ctgaatgcgg aaatcgtctt cgttatgtct
     7981 cagggcactg acaccccgga acagctgaaa gagcgtatcg aactgacccg caacagcttc
     8041 ggtggtgcca aaaacaccaa catcaccggc gttatcgtta acaaactgaa cgcaccggtt
     8101 gatgaacagg gtcgtactcg cccggatctg tccgagattt tcgacgactc ttccaaagct
     8161 aaagtaaaca atgttgatcc ggcgaagctg caagaatcta gcccgctgcc ggttctcggc
     8221 gctgtgccgt ggagctttga cctgatcgcg actcgtgcga tcgatatggc tcgccacctg
     8281 aatgcgacca tcatcaacga aggcgacatc aatactcgcc gcgttaaatc cgtcactttc
     8341 tgcgcacgca gcattccgca catgctggag cacttccgtg ccggttctct gctggtgact
     8401 tccgcagacc gtcctgacgt gctggtggcc gcttgcctgg cagccatgaa cggcgtagaa
     8461 atcggtgccc tgctgctgac tggcggctac gaaatggacg cgcgcatttc taaactgtgc
     8521 gaacgtgctt tcgctaccgg cctgccggta tttatggtga acaccaacac ctggcagacc
     8581 tctctgagcc tgcagagctt caacctggaa gttccggttg acgatcacga acgtatcgag
     8641 aaagttcagg aatacgttgc taactacatc aacgctgact ggatcgaatc tctgactgcc
     8701 acttctgagc gcagccgtcg tctgtctccg ccagcgttcc gttatcagct gactgaactg
     8761 gcgcgcaaag cgggcaaacg tatcgttctg ccggaaggtg acgaaccgcg taccgttaaa
     8821 gcagccgcta tctgtgctga acgtggtatc gcaacttgcg tactgctggg taatccggca
     8881 gagatcaacc gtgttgcagc gtctcagggt gtagaactag gtgcaggcat tgaaatcgtt
     8941 gatccagaag tggttcgcga aagctatgtt ggtcgtctgg tcgagctgcg taagaacaaa
     9001 ggcatgaccg aaaccgttgc ccgcgaacag ctggaagaca acgtggtgct cggtacgctg
     9061 atgctggaac aggatgaagt tgatggtctg gtttccggtg ctgttcacac taccgcaaac
     9121 accatccgtc cgccgctgca gctgatcaaa actgcaccgg gcagctccct ggtatcttcc
     9181 gtgttcttca tgctgctgcc ggaacaggtt tacgtttacg gtgactgtgc gatcaacccg
     9241 gatccgaccg ctgaacagct ggcagaaatc gcgattcagt ccgctgattc cgctgcggcc
     9301 ttcggtatcg aaccgcgcgt tgctatgctc tcctactcca ccggtacttc tggtgcaggt
     9361 agcgacgtag aaaaagttcg cgaagcaact cgtctggcgc aggaaaaacg tcctgacctg
     9421 atgatcgacg gtccgctgca gtacgacgct gcggtaatgg ctgacgttgc gaaatccaaa
     9481 gcgccgaact ctccggttgc aggtcgcgct accgtgttca tcttcccgga tctgaacacc
     9541 ggtaacacca cctacaaagc ggtacagcgt tctgccgacc tgatctccat cgggccgatg
     9601 ctgcagggta tgcgcaaacc ggttaacgac ctgtcccgtg gcgcactggt tgacgatatc
     9661 gtctacacca tcgcgctgac tgcgattcag tctgcacagc agcagtaatc tcgtcatcat
     9721 ccacagcttt gcgctgcgga tatctgaacc ggaaataatc actatttccg gttttttatt
     9781 ctcttaattt gcattaatcc tttctgatta tcttgcttaa ctgcgctgca tcaatgaatt
     9841 gcgccatctc actttgcata cttaccactt tgttttgtgc aagggaatat ttgcgctatg
     9901 tccgcaatca ctgaatccaa accaacaaga agatgggcaa tgcccgatac gttggtgatt
     9961 atcttttttg ttgctatttt aaccagtctt gccacctggg tagttccggt ggggatgttt
    10021 gacagtcagg aagtgcagta tcaggttgat ggtcaaacaa aaacacgcaa agtcgtagat
    10081 ccacactcat ttcgcattct gactaacgaa gcaggcgaac ctgagtatca ccgcgtacag
    10141 ctgttcacga cgggcgatga acgcccgggc ctgatgaact tcccgtttga agggttaacc
    10201 tcaggatcga aatacgggac agccgttggc atcatcatgt ttatgctggt gattggcggc
    10261 gcgtttggca ttgtgatgcg tacaggaacc attgataacg gtatcctggc gcttattcgc
    10321 catacccgcg ggaatgaaat tctctttatt cctgcgctgt ttattctgtt ttcacttggc
    10381 ggcgcggtat ttggtatggg agaagaggcc gtcgcctttg ccattatcat cgcaccgcta
    10441 atggtccggc tgggctatga cagtattacc accgtcctgg tgacttatat tgccacgcaa
    10501 atcggttttg ccagttcgtg gatgaacccg ttttgtgtgg ttgttgctca ggggattgcc
    10561 ggcgttccgg tgctttctgg ctccgggttg cgcatcgtgg tgtgggttat cgccactctg
    10621 attggcctga tctttaccat ggtgtacgcc tcacgagtga aaaagaatcc tcttctgtca
    10681 cgcgtgcatg agtccgaccg cttctttcgt gaaaagcagg cggatgttga acaacgtccg
    10741 tttacctttg gtgactggct ggtattgatt gtcctgacag ccgtaatggt ctgggtgatt
    10801 tggggcgtga tcgttaatgc ctggtttatt ccagaaattg ccagccagtt cttcaccatg
    10861 ggtctggtga ttggcatcat cggcgttgtt ttccgcctta acggcatgac ggttaatacc
    10921 atggcttcat cctttaccga aggggcgcga atgatgatcg cccctgccct gctggtgggt
    10981 ttcgccaaag ggattttgct gctggttggt aatggtgaag caggtaatgc cagcgtgtta
    11041 aatactatcc tcaacagcat tgccaatgcc attagcggtc tggacaacgc ggtcgcggcc
    11101 tggtttatgt tgctcttcca ggctgtattt aatttcttcg tgacgtccgg ttctggtcag
    11161 gcggcattaa ctatgccgtt actggcaccg cttggcgatc tggtcggtgt taaccgtcag
    11221 gttaccgtgc tggcgttcca gtttggtgat ggcttcagcc acatcattta cccaacctca
    11281 gcttcgttaa tggcaacgct cggtgtgtgc cgggtggact tccgtaactg gctgaaggtg
    11341 ggcgcgaccc tgcttggact gctgtttatt atgtccagcg tcgtagtgat cggcgctcag
    11401 ttgatgggct accactaaaa atgttaagag ccgcaattgc ggctcttttt tcattctgcc
    11461 gtttcagtct ctacggcttc atttttggca ttgcgcttca tccacaacgc cagcgctttc
    11521 agcgagtctg gagtgaactc atcgcagcgt gcggtgattt cttccggcgt cagccagcaa
    11581 actccactga cttcatcttc ctgtagggcg aagggaccgt gagagacgca actgaacaac
    11641 gcgccccaga cacggcaatt tttatcttcg aaatagaact gcccgtgctc ggcaaagggg
    11701 acaccggcaa tgcccaactc ttcttccgct tcgcgacgcg cggattccag cagttgctca
    11761 tcggcctgaa ctacaccgcc tgcggtcgca tctaacatgc cgggtaaaaa gtcttttgtc
    11821 tcggtacgac gctggaccag aattttgccc atgccatcat gcacgacgat gtaagttgca
    11881 cgatgacgca gacactgtgc ccgcatttgt tcccggctgg cttgtgcaat gacttcgttc
    11941 tcttcattga caatatccac ccattcagta cttgccaaac gacgctgttc cattatcagg
    12001 aaaccttctt tttctggcgc tcttacggcg cattttgagt tgtgggtaaa ttacggatta
    12061 atcgcgacct gcgcaatgat actttgatca ttgagtgcga ttacactaag tacgtcatta
    12121 tccagcatgc cataacccgc cggattaccg cctttcggaa tactcaccga gccggggttg
    12181 aagtggaaaa tctccccccg ctgttctgcc actggcagat gggtatgacc gtacaccagc
    12241 acatcgttct ggtttaaagc aggtagattt tccgggccaa aaagatgacc atgcgtcaaa
    12301 aacagacgtt gtttttccag taatacctgt tgccacggcg cggttatcgg gaaatgcagc
    12361 atcatttgat ccacttcgct gtcgcagttg ccacgcacag cgataacctt atgtgccact
    12421 tcattaagcc gttcagcgac tttggctggc gcgtaaccct ccggtaaagc attacgcggg
    12481 ccgtgattca acacgtcgcc aaggatcacc agccactggg caccgctttg gacaaacaac
    12541 tccacaacac gttccgtcgc cggtaacgac ccatgaatgt ccgatgcaaa catcagtttc
    12601 atcactcact cctcgtcgaa aaaacgtaag ctatgatact ggattctgtc gttgctatca
    12661 gccagattgc ttcgccgaaa gtgcaataaa acgctggaca gacgctcgct gccactggaa
    12721 tgtcgcataa tccaccaggc gttctggcac ctcatcgcca tgtagtacca ggcggttaat
    12781 cattagcgcc agatcagtat cagcaatgca ccattcacca aataaattcg gctgacccag
    12841 tgctaacaaa tgttctgcca tcgcgaacag tttctctgca ctggcttttc cctcggccgt
    12901 tagtggcgct tttttcgccc ccgcaaagac aacatccgtc ggacgctctt cgcggatggg
    12961 catcagatcg ctgcgcagcc aggcctgaat ctgtcgtgca cgcgcacgat tttctaaatc
    13021 aagcggataa atacgttccc aggtcggtgg cgcaaatcga tcttccagat actccgcaat
    13081 ggcagaagat tcactcaact caaaatcatc gatttgtaat aacggcacac ggcgtgtctg
    13141 accgtaacct tgccacgtcg gctgcaaatg ttcaccgctg tcgaggtcga tggtcttgat
    13201 atgaaacgac aggccttttt cctgcaacgc cacccaggcg gataacacat aaggggagaa
    13261 aaagtgggca tctgaccaaa gcgtgatagc gggtttactc attatgtcct cggcaataaa
    13321 gggggctggc tatcaaacta tagagccttt ttgtcactgt cacgtgacga atactcacgt
    13381 aagtcgccgc gctttctata cttgtggtgt tcagatgatt aaacaaccgg agctgcaatg
    13441 atcgatctct atttcgcccc gacacccaat ggtcacaaaa ttacgctgtt tctcgaagaa
    13501 gcagagcttg attatcgctt gattaaggta gacttgggga aaggcggtca gtttcgtccg
    13561 gaatttttgc gcatttcgcc caacaacaaa attccggcaa ttgttgatca ttctcctgcc
    13621 gatggcggcg aaccgttaag cctctttgag tctgctgcca ttttgttgta tctggctgag
    13681 aaaacaggac tctttttgag tcatgaaacg cgtgagcgcg ccgccacatt acagtggtta
    13741 ttctggcagg taggcggact ggggccgatg cttgggcaaa atcatcattt taatcacgca
    13801 gccccccaaa ccattcctta cgctattgaa cgttatcagg ttgaaactca gcgtctttac
    13861 catgtactaa acaagcggct ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt
    13921 gcggatattg cctgctggcc gtgggttaat gcctggactc gcctgcgaat tgacctcgca
    13981 atgtatccgg cagtcaagaa ctggcatgag cggatccgtt cgcgccctgc caccgggcag
    14041 gcactgctaa aagcacaact cggtgatgag cgttcggata gttaacagaa acaggttctc
    14101 gtgtattatt tcatcctaag taaaacaacg gagaacctgc aatggcacaa cctgccgcta
    14161 ttattcgtat aaagaacctt cgtttgcgta cgtttatcgg aattaaggaa gaagaaatta
    14221 acaaccgtca ggatattgtt atcaatgtga cgatccacta ccccgccgat aaagcgcgca
    14281 ctagcgaaga tatcaacgat gcgctgaatt atcgcaccgt aacgaaaaac attattcagc
    14341 atgtagagaa taaccgtttc tctttgctgg aaaaattaac tcaggatgtg ctcgatatcg
    14401 cacgtgaaca tcactgggtg acgtatgctg aagtggagat cgataaactg cacgcgctgc
    14461 gctacgccga ttcggtatcc atgaccttaa gctggcagcg ttaatcgcca tatcgggagg
    14521 ctgcatgaac atagtgatca ccggagggac gggattaatt ggtcgccatt tgattccgcg
    14581 tttgctggag ctgggccatc aaattacggt agtgacgcgt aacccgcaga aagccagttc
    14641 cgttctcggc cctcgggtga cactatggca agggcttgcc gatcaaagca acctcaacgg
    14701 cgttgatgcg gtaatcaacc tggccggaga accgattgct gataaacgct ggactcacaa
    14761 gcaaaaagag cgtctctgcc aaagccgctg gaatatcacg caaaaactgg tcgatttgat
    14821 taatgctagc gacacgccgc cgtcggtact catttccggc tcggcaacgg gctattatgg
    14881 cgacttaggt gaagtggtgg ttaccgaaga ggaaccgccg cataacgaat ttactcataa
    14941 actctgcgcc cgctgggaag agattgcctg ccgggcgcaa agtgacaaaa cgcgagtgtg
    15001 cctgctgcga accggcgtgg tactggcacc ggacggcggt attctcggta aaatgctgcc
    15061 accgtttcgt cttggcctgg gcgggccgat tggttccggt cggcagtatc tggcctggat
    15121 tcatattgat gatatggtca acggcattct ctggctgctg gataacgagc tgcgcgggcc
    15181 atttaatatg gtttcgccct acccggtacg caatgaacaa tttgcccatg cgctcggtca
    15241 tgcgctgcat cgcccggcta ttttgcgcgt ccccacaacc gccattcggc tgttaatggg
    15301 cgaatcttca gtactggtat taggtggaca acgcgcgctg cctaaacggc tggaagaagc
    15361 gggttttgcg tttcgctggt acgatttaga agaggcgctg gcggatgtcg ttcgctgatg
    15421 tggtttacag caaacatccg ccagttaact ccctgtgtta caggattagt ggctttgcgc
    15481 gataagatcg tctggtgaaa gtcgggtcac catcataact aactctctgt ctaaacctct
    15541 atccagcatc tcctgagcaa tacgcagggc ttcttcgtgt ttgccctgca ttgcgccttc
    15601 ttcacgtaat ctgtcagcaa tggtcatcag tttctccttt tcttgtggtg cgcgttctgt
    15661 tatctcacca ataaatgcac ggaaacgctg ggcatcccct gtttgtaata cgtaattaaa
    15721 cagggctttt agctgtctgt cattagtgtt ccctgtaact agcagcgaaa caatttggtc
    15781 gactaatccc aacagatcgc gctgacgaat atgtttctga attaactcca acagcgccat
    15841 tttgcggtgt tgcataatct cgtcatccgg caccacggta atatccacca acggaaacgc
    15901 tgatgaatat attttgcggg ctatagcagg ctcggcaaat tcatcaagcc agcagagtga
    15961 ataaggataa ggacttctgc aaccatgata aaacagcatc gggatcacca atggaagctc
    16021 tttatagccc gcatcaagat ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa
    16081 agccattaat tcttccggct tactttggtg ctctatcact acataaatat aacccactcc
    16141 ctcctgcgtt ttcacagacc acaagaggtc ggaataatat tgccgcaggt cttcatcaat
    16201 aaaactgttt ggttctagtt taagcgtcgt taaatcacac agtttgcgca gcggcgcggg
    16261 aagatgaata ttaataaaat tccgcgcggt gtctggatgg cgtaaaaaag atttaaatac
    16321 cgcatcatgc ggcgtggaag ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt
    16381 aagatgacgc gacaataacc acgattaaca cccaatgcag caggcagttt ttatctttgg
    16441 acagcgaatt cagaagtatt tttagcggac tgcaactttt acatttcagc ggaagctgac
    16501 tcgcaaattc cccttgaaaa tactccggca gcgttagcca ccggagagga aattatttca
    16561 gcgatccctt aaggaactgt tgcagacgag ggctttgcgg gttgccaaat aactgctccg
    16621 gcgcgccctc ttcttctatt ttcccctgat ggaggaaaat gacgtgagta gaaacatggc
    16681 gagcaaagcc catttcgtga gtcactacca ccatggtttt cccctcttct gccagttgct
    16741 gcataatacg taacacttcg cctaccagtt caggatcgag cgccgaggta ggttcatcaa
    16801 acagtaaaac ttctggttcc atcgccagcg cccgcgcgat agaaacacgc tgttgctgac
    16861 cgccggaaag atgcaccgga tatttcccct gcgcacgttc gtctatcccg actttcgcca
    16921 gatacttcac cgcccgctcg cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg
    16981 cttccatgac gttttccaac accgtcatat ggctccagag attgaagtgc tggaatacca
    17041 tcgtcaggcg tgtgcgcagt aagcgcagtt gatttttatc ggcgactttg agttgaccgt
    17101 ctttgtcgcg caccagattg atcgtctggc cattgaccac gatcgatcct tcactcggtt
    17161 tttcgaggaa gttaatgcag cgcagaaagg tacttttccc cgatcccgac gatccgatga
    17221 tgcttattac atctccggca ttcgcttgca gtgagacccc tttcagcact tcgtgttcgc
    17281 catagcgttt gtgcaaatcg ataacgttta atttattctc ggacatcgta ttctcagtgc
    17341 gttgaagaag gtttcacatg ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc
    17401 aggacataag agatgattaa atagagcacc gcggcaatgc cgaaggcggt aaaaggttga
    17461 tacgtggcgg cgttaatatc gcgggctatt ttcagcagat ccggcacagt ggccgtaaac
    17521 gccagcgcag tagagtgcag catcaggatc acttcgttgc tgtatgccgg taacgcaata
    17581 cgcagcgcag aaggcaaaat aatgcagcga tacattttaa aggtcgagaa gccataggct
    17641 ctggcggctt caatttcccc atgcggaacc gaacggattg ccccagcaaa aatctcggta
    17701 gtgtaagcgc aggtgttaag cgtcagcgcc agcacggtac agttcaggcc actgcggaag
    17761 aaagcgttaa ggaattcggt tcccttaaca atctcaagcg tgtacatgcc ggaatagaac
    17821 accagcaact gaacatacag cggcgtaccg cgaaaaatat aggtaaataa ccagattgga
    17881 aactggatgt atttattact ggagacacga ccaatcgcca gaaacagcgc caggactccg
    17941 cctatcacta ccgacaaaat aagcagccac agagtgatcg ccacaccagt aaagcgataa
    18001 ccgtcggtcc acagcagcgg tttccagtat tcatgtaaga tttcgatcac aggtcagccc
    18061 tcttcacacc cacggagtag cggcgctcaa ggaacagcag cacaccattg gaaacggtgg
    18121 tgaaaaccag gtaaatcacg ccacagacga tggcgaaata gaacggttcc caggtacttt
    18181 tgcctgccag ctgcgtggct ttgaccacat cttccaggcc gagtaacgaa accagtgcgg
    18241 tagatttgag gatcacctgc cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg
    18301 ccggaaacat gatccgccga aacacttgcc cgcgagtaaa accgaacgcc gtcgccgcct
    18361 ctatatgccc tttcggcact gccataaacg cgccacggaa cgtttcggta aaataagcac
    18421 cgtagataaa accgagagtg ataataccag cgaccatcgg atcgatatca atctgcccga
    18481 cgcccatcgc ctccgtcacc atgtttagcg caatctgcaa accatagaaa atcagcagca
    18541 tcaacaccaa gtccggcacg cctcgaatca gcgtggtgta cccttcgaaa atcagccccg
    18601 aaagccgatt ttgcgagagc ttaccgccag cgccaattaa accgatgatt acagcgagca
    18661 ctacagagct gatagccagc tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa
    18721 acccatacaa catgctgcct gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct
    18781 ccggttaccc gaagggagcg ggctttcttt caccgatggc gattaaccac cataaacatc
    18841 aaaatcgaag tactttttcg ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa
    18901 ggctttgttc agtgcctcgc gcagttcgtt atcttcttta cgcaggccca tgccggtccc
    18961 tacgccaaac agtttttcat ctttaacaga cggaccaccg aatttgtaat ctttaccgac
    19021 gggttgtttg aggaaacctt cgctggcagc gacctcatcc tggaacgcgg catcaatacg
    19081 tccggcagtc aggtcagaat aaatgttgtc ctgcccctga tacgagacga tttcaatgcc
    19141 ttttggtgcc cagtgttcat taccgaacgt ctcctgggtg gtgccctgca atacgcctac
    19201 ccgtttgcct ttcagcgact cgactgtcgg ctgaatgtca gaatttttcg ccaccaccag
    19261 acgagaatcg gcagcgtaca gtttgtcggt gaaggcaatt tcttgctgac gtttttccgt
    19321 aatggaaagc gatgacatga tggcgtcaat cttcttcgct tttaaggacg ggattaacgc
    19381 atccagcggg ttttcgacaa acgtacattg cgtattgatg cgcttgcaca actccttcgc
    19441 cagctcgata tcgaaaccaa ccagttcgcc ttgtgaattt tttgattcaa atggcgcata
    19501 ggtcgggtcg gtaccgatgc ggatgttttg cggaattgca gcaagtgccg cagttgcgct
    19561 ggagaaggcc agaaccagag agagcgatag caccagtttt ttcataatgt cctcaactga
    19621 cagtcttttt atagggaata tttacaggtt tgttacactt atcgtgccat aaaattgacc
    19681 atcaaggcaa aatatcccgc cccgttagac gtattatttt tcattaaaaa tgcttaagta
    19741 tgcatttcaa tgcaccacga tagtgcaaca aacctggcgt gcaccatgat gatcattcca
    19801 tcaggtacag cttcccagcg acgtatcagt caccgtagac attaaagtcg aaatactttt
    19861 tcgccatctt gtcgtaggtg ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag
    19921 ccgtcagttc agcataatct ttacgtagcc ctacaccggt gccatcaccg aagtattttt
    19981 tgtcttttac tgatgagcca gcaaaagcga aatctttacc agcaggttgc ttgaggaatc
    20041 cttcgctggc agcaacttca tcttgtaacg cagcatccag acgtcctgca gccagatcgg
    20101 aatagaccaa atcctggttg gcataggcca ccacatccac gcctttacta cgccaggtct
    20161 cgttagcgta agcttcctgg gttgatccct gcagcacacc aacatgttta cctttcagtg
    20221 aatccagcgt tggctgaatc ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt
    20281 acagcttgtc ggagaaggca atctcctgct gacgtttatc ggtaatggaa agcgacgaaa
    20341 taatagcgtc gatttttttc gctttcagtg aggggatcag cgcgtcaaag tcactggcaa
    20401 cccaggtaca tttcacctgc atccgtttgc acatctcgtt accgagatcg atatcaaagc
    20461 caacaaaatc acctttagca tctttcgacg agaacagcgc ataggtggta tcggttccga
    20521 tacgtaccgt ctccggtagc gccgcatagc tggaagccgc tgcggagaga ccgactaata
    20581 aagacagagc gagaatcgac ttcttcatac ataaccctca agtgaaaatg gcttttttat
    20641 gttgtgtatt gtgttgtgtg tttgcaggct ctttcatgca ggtcttatgc catcttgacg
    20701 acaacagtaa cattcaacgt taaatatgtt aataagacat tgcattattg tcctgaagtt
    20761 gaagatagca ggtatagcgg ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg
    20821 tcgaggattt gatcgcggtt gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg
    20881 ccctgccagc gggcaaagag atcttcagga agggttattg caaactggtc aagaacacga
    20941 ttaaccgtct gatttatcac atcatcaagg gattgcgggc gatgataaaa cgccggaacg
    21001 ggaggcataa tcaccgcacc gatttctgcc gcctgagtca ttaaacgcag atggcctaag
    21061 tgcaatggtg tttcacgcac gcagagcacc aacgggcgac gctctttcag caccacatct
    21121 gccgcacggg tcagtaagcc atcagtatag ctatggacaa tgccggacag ggttttgatt
    21181 gaacagggta aaatcaccat ccccagcgtc tggaaagaac cggaagagat gctggcggaa
    21241 atatcgcgcg catcgagcgt gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc
    21301 gtttcgaggg ataaggtctg gcgcgccgcc tggctcatca ccagatgcgt ttcgatatct
    21361 gtgacatcgc gcagaacctg taataagcgc acgccataaa tcgcgccgct ggcaccgctg
    21421 atgcctacaa tgagtcgttt cataaatagt tgccctgtca gacttgcagg cagactttgc
    21481 agtatttcgg cgggagttgc aagtcagggt gccagaccgg caccctcagc gaaggcatca
    21541 tccttcgtta tgcatttcga gattttccac ttcgttctga cgttgcactg ctttggcgtc
    21601 atcattacgt aacgtatcga ggaaatcgag gtagccctga tcaacatctt tggtgacgta
    21661 gacgccgttg aacaccgagc attcaaactg ctggatatcc ggattttcag cgcgaacggc
    21721 gtcgatcaga tcgttcagat cctggaaaat caacccgtca gcaccgatga tctggcgaat
    21781 ttcatcaact tcgcgaccgt gagcgatcag ttccgtggcg ctcggcatat caataccata
    21841 aacgttcggg aagcgaattt ccggtgccgc agaagcgagg tacactttct tcgctccggc
    21901 ttcgcgtgcc atctcgataa tctgctcaga agtggtgcca cggacgatgg agtcgtcgac
    21961 cagcaggacg tttttatcgc ggaactcggc gcggttggca ttcagtttac ggcgcacgga
    22021 cttacgacgc agctgctggc ccggcatgat aaaggtgcgg ccaacatagc ggtttttaac
    22081 gaagccctgg cggtacggtt tgcccagaat acgagcaatt tccagcgcga tatcacacga
    22141 ggtttctggg atcgggatca ccacgtcgat atccagatct tcccattcgc gggcaatttt
    22201 ctcgcccagt ttcgtgccca tattcacacg cgcgctgtaa acggaaattt tgtcgataaa
    22261 cgagtccggg cgggcaaagt atacatactc aaacaggcac ggattgctga ccggattgtc
    22321 agcacattga cgggtaaaca actgcccttc ttcagtgatg taaatcgctt cgcccggcgc
    22381 gacgtcacgc aggaaatcaa agcccagcgt atcgagcgct acgctttcgg aagcgaccat
    22441 atattctgta cggttctcgt caatatcacg ttttcccagt accagcggac gaatcccgtt
    22501 tggatcgcgg aaagcaacca taccgtggcc gataatcatc gccacacagg catacgcgcc
    22561 gcggattaag cggtttgtgg cagcaatggc agcgaaaata ttgtcggctt ccagcgggta
    22621 gtggcggaag ttgtccagct cgctggcgaa gatattaagc agaatttccg agtcggaagt
    22681 ggtgttgatg tggcggcgtt tttcttcaaa cagtttttta cgcaactcgt gagcgttggt
    22741 cagattgccg ttgtgggcaa gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc
    22801 ttcagaggcg ctggagctgc cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc
    22861 ctgcaaacgc tgcatatggc gagcttcaaa tacatcgctc accagcccgt tcgctttacg
    22921 caaacggaag cagttattgg catctatggt gatgatgccg gcggcatcct gaccgcgatg
    22981 ctgaagcacc gttaaggcat cataaatcga ctggttaacc ggcataacac cggcgatacc
    23041 gacaataccg cacatacgtc tttttcctcg ttaagccaca tctcagagca cttacgctct
    23101 gggcaagaaa cttgacgagc tttgcagata atcaaaaaac catctgatga taaaactgaa
    23161 ttgcgggatc agctgtgatt tgctccagtc ttcacttttc gacaccccgg taaaggagtc
    23221 gagaaagaag agaatggcgg caacaatcaa cacaccgcgc aacgcaccga aacagacgcc
    23281 cagcacccga tcggtgcctg acaaccccgt tttctccacc aactggccta tcacgaagtt
    23341 cacgatagca ccaacgatca gggtagcgat aaacagtacc gcgatggcaa tcccatttcg
    23401 aaccagttcg tcttcaaagc ccgtaaacca gactgacagg taagtgtagt aatgactggc
    23461 aacaaagaaa gcacaacccc atgtcaccag cgataacgct tcacgaacaa agccgtggat
    23521 caggctaacc agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa tccagaccat
    23581 atgtgtccca cgattttacg ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga
    23641 aaacgtttgc gtagggattt ccttcccgcg catcaataaa aatggcgctg aaaaaatatt
    23701 caacgccatc gactttttat gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta
    23761 aacgccttat ccgacctacg gttctaacac tgcccaagcc tgataagacg cgccagcgtc
    23821 gcatcaggca agactgtatt aattcggcgt atagcccatt accacgccac ttaagccaga
    23881 aagttgcttc aactcaccca gcgaaccttt cagcttatct ttcgaggcat ccggcccaac
    23941 cagaatacgg gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc
    24001 ggcaccgcgc agcttaccga caatctcatt cactttatcg gcatttttca gcgcacccag
    24061 ttgcacaaca taggctttac ccgttggcgc tgctttttct tccacgaccg gcttcggttc
    24121 tggcttcggc gcaggtggta cttcaacctt tggtttaggc ggctccaccg gtttcggctt
    24181 cggtggggcg accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggc
    24241 cggatcgagc gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg
    24301 cggctgcgtc ggcaacgcct gggtggcggc tggcatcata tcaggctcat cacgatcgcc
    24361 cgctttcggc accagcggga tagccgcgaa ctcatcctga taatgttttt tctgcccgtc
    24421 cagcagccct ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg
    24481 attctgaaac ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga
    24541 ccgtgtggaa agaaccacac accagcacgg tgtcttccgc tttagcgtcc gccattgcgg
    24601 catcccatgc ctgcgcaacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt
    24661 gttctgccgt ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc
    24721 ttttcaacca ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca
    24781 gcacgcgccc gtttttcggt agcgctttca tacgcccggt gagatattcc gccgcatgtg
    24841 gattatgcgc gacatcaaaa ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg
    24901 gcaaaattgc gctggcaatc ccgtcgcgaa tggcattttc actgacttcc agcccgctgg
    24961 cacgcagtgc cgccagcgcc gttgcggcat tcggttgcgg gacaagcggc aacggcagat
    25021 tttccagcgt gccgtgagca tcgctaaacg cccagtcatg atcggtgacg gaatagttcc
    25081 actcaacgcc ccgacgttgt aacagtgcac ctttttcctg cgccacatca gcaatggtag
    25141 aaggcatttc cggctcaccg acaattgccg gtttttcgct gcggaagatg cctgctttct
    25201 cgagaccaat actttcgcga tctggaccca gccagtcggt atgatccagc gcaatactgg
    25261 ttactaccgc gacatcggca tcgacaatat tggttgcgtc cagacgaccg cccagcccta
    25321 cttccagaat caccacgtca agttgtgcct gcttgaacag ccacaacgcc gacagcgtac
    25381 cgtactcgaa ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg
    25441 cggtgtgggc cgattccggc aattcctggc cctgcacacg tacgcgctcg gtataacgca
    25501 ccagatgagg cgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca
    25561 gcgtacggca ggtggtgcct ttgccattcg tacccgcaac ggtaaacaca aatggcgctg
    25621 gtttcaggac gccaagacgc gccgcgacct ggctcacgcg ctcaaggccg agatcgatag
    25681 ttttactgtg caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag
    25741 gagtgcgttt gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca
    25801 gagccagtgg ccctgccctt atcagttatc aggcctcagg ttcctgatcc ggtaccgggg
    25861 gtaccactac gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg
    25921 ccagaatgct cgccagtttc aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc
    25981 ctttctcgat caggaattca ctgcgctgga atctaggcgg cagtttttcg cgaacggtct
    26041 gttcgataac acgcggaccg gcaaagccga ttaacgcttt aggttcagcg atgttgagat
    26101 cgcccagcat ggcgaaactt gcagaaacac cgcccatcgt cgggtcggtc agcacggaga
    26161 tgtacggcaa gccgcgctcc tgcatttttg ccagtgccgc agaggttttc gccatctgca
    26221 tcagcgacat cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagcg
    26281 ggcagttatc ttcctgcgcc tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc
    26341 ccattgaacc gcccataaag gcgaactcga atgccgcagc gacaaccggc attccataca
    26401 gagtgccttt catcaccacc agcgcatctt tttcgccggt ttctttctgc gcagatgcca
    26461 gacggtcttt atacttcttg gagtcacgaa acttcagctc atctttcggc tcaagctcgc
    26521 tacccagctc cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca
    26581 tacgcatgtg atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt
    26641 ataaaacctg accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc
    26701 gggtgggagt aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct
    26761 gaacctggtt cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac
    26821 agaccatgaa tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc
    26881 gaaccgcgga gttacttttt attttgcggc gcgtgccgca gcgcgtttgt gacggattat
    26941 ttcgatgacg cccggcaaaa tagaaaccac aataatcccg acgatcagca gcttaaggtt
    27001 atcctgaacc atcgggattg taccgaagaa atagcctgcg taggtaaaaa gcagtaccca
    27061 caacagtgcg ccgatcacgt tataagcagc gaaatgacgg tacgacatgt ggcccattcc
    27121 cgcaacaaac ggggcgaacg ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt
    27181 gccgccatgt ttctcataaa actgatgggt tttgtcgaga taactgcgac ggaaaatttt
    27241 cgaattggga ttactgaata acttttcacc gaacaaccgc ccgatagtgt aattgaccgc
    27301 gtcacccaca atcgcggcga tcagcatcag cactaccatc atatggacgt tgagatcgtt
    27361 ggtttccagt gatgccaacg ctccggcaac aaacagcaat gaatcacccg gtaaaaacgg
    27421 cattaccacc agaccggttt cacagaacaa aattaagaac aaaatggcat aaacccagac
    27481 gccgtactcc gcgactagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag
    27541 gaaataaatc aggtccatat ttgcctatgc cttgtactcg tcatctttca ggctgtaact
    27601 acgttggcta cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg
    27661 ggcccattgg cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca
    27721 gcccttccgc ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg
    27781 ctatccagct ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc
    27841 tgaccatatg atgtacaaag gcattcgctt tgatatcaac caccacataa ggaccgtgac
    27901 gcgtgacatt aatatgcata acgttgcgcc acggggttcg ggactggcac tgcaccgcac
    27961 ggaacgaggt gaaatcattc tcgcccagca agcattgcgc agcccgatgc atccgttcag
    28021 cgtccagcgg ttcgtaaaaa tgggttaccc ctttactcag taccgccggg cgcagccgat
    28081 gattgtagat gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat
    28141 caggtacagc tttaacccaa cgcacggcga tgtcaccagg taaattcgca tttacgccca
    28201 aggtccacgc cgcgtctttg cgctgcgcgg ttgtttcgaa atgcaccact tgcccggtac
    28261 cgtgtacccc tgcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct
    28321 gggagagcgc cttttccagc ttctcctgca cactgcggac ttcattctgc cgttgccagc
    28381 cgtaatactt actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcagttgtt
    28441 gctggtcgga cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat
    28501 cagcgcgccg ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat
    28561 accgtagtca ttacgcacgc agccaacaga aagatgcggc gtacccgaag catcccctac
    28621 ctgagtcggg aattcgttct cttcagagag cacaatatct tcgccctgag caaacgcatc
    28681 acgcgcttct tctgccgcca gcggacgcag tgcttcaaag ttgaccatct gggcatgacc
    28741 gtagaatacc ggtgcctgga cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat
    28801 tttgcgtact tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa
    28861 caacggcagc atattgaacg ccagttgacg accgaagaaa tcttcttcgt caatcggaat
    28921 gccgttgagc aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc
    28981 ggaggctgaa atcaggctgg taacactgat acgcgacaaa ccgccctgat cgattaacgg
    29041 tttcagtgcc gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt
    29101 gcggtaatcg gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc
    29161 gagagcaaac agtccgctgc tgtcgatcac caggcaacct gagttggtcg cttcttcaac
    29221 ccaggcagcg gtagcttctt tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc
    29281 gaattcagcg gcatcctgca cggtgattgt tttaccacca aagcgcagtt gttcgcctgc
    29341 gctttcgtta cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt
    29401 ttcaagcagg gcttcgccca tagcgccagt tgcgcccagg acggcaatgt tccagccttc
    29461 agacatggtg gtttactcca gaaatagcaa agctccctgc caatgttaca gcagagagca
    29521 tgaagaagag attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat
    29581 gatgccgcac tggcgtcatc acaaattaca tacagagatg accattcacg gcgctcaaga
    29641 tagtttttgc gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg
    29701 tcatcgcggc gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc
    29761 ggaccatgca gcgtaatgcg gccaaactca ggcgcgggca gcaacgtatc cagcgcaacg
    29821 tgttgttcat gcccaataaa cttgctatag gcttcaaaca cttgcgtcgt gccacgcgct
    29881 ttaccctcca gggtatagcc tgcgatatgc ggcgtgccga tatccacttt tgtcagcaat
    29941 tccacattaa gttccggctc gccttcccag acatccagca ccacgcttaa cttctggcct
    30001 tcattcaggc aggtcagcag cgcggtatta tcgacgactg cgccacggca ggcgttaatc
    30061 agaatcgctc cgggcttcag gctacggatc agtttttcat ccgccagatg tagcgttttg
    30121 tacggaccat ctttaaagag tggcgtatgg aaagtcagaa tatccgcgcg ctggactaac
    30181 tcatccagcg agcggaaatc accctcatcc ccacggtcgg cgcgaggcgg atcgcaaagt
    30241 aaggttttaa tccctaacgc ttccagtcgc gcctgcaatc gacgtccaac gttaccaacg
    30301 cccacgatcc ccaccgtacg gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagc
    30361 agggaggaga aaacatattc caccaccgca atcgcattac agccaggtgc agcggaaaaa
    30421 ccaattcccg cctgcttcaa ccatgcttca tcgacatggt cggtccccgc agtggcagtt
    30481 ccaacaaatt taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc
    30541 accatcagcg catccgcgtc tgccagttga gcgacgggga ttggacgccc gggaaccgcg
    30601 atcacctcac ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg
    30661 attttcacgt ttgtgttacc tgtatgagac gagagttaac cggacaagta tgccataatc
    30721 tcgcggccag gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat
    30781 ttccggccct cctcctgggc agccaccagg tcagggagat aacctgccgt ctggcgcggg
    30841 caatcagcct ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac
    30901 ctcactgacg caacaacaaa gagcagaact gtgggcgggt atcaggcacg atattggtct
    30961 gtcgggagat tcaccgctgc tttcgcgtca cttccctgcc gctgagcata atctggcgca
    31021 acgtctgctg gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga
    31081 gtatttacgt ctggggaatc atcgtcaggc ggtcacggat tatatccgtc ataactttgg
    31141 tcagacgccg ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca
    31201 ggaagggaag atggttattc cgcaaccaca gcagcgcgag gcgaccgacc gtcctttatt
    31261 accggcggag cacaatgcgc taaaacagct ggtgaccaaa cttgcggcgg caacggggga
    31321 acccagcaaa cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt
    31381 aattccagcg aaactgttta accatctggt gacctggcta caggcgcgtc agacgctaag
    31441 ccagcaaaat acgccgacgc tggaatcact acagatggcg ctaaaacaac ctttagatgc
    31501 cagtgaactg gcggcgttat cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc
    31561 atcgctttct tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa
    31621 agggattgat ccgcgtgata tgcaaccgct gcttaatcct tttccaccga tgatggacac
    31681 gttgcaaaat atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat
    31741 gctggtctgg ctggttcgtt aaccccgacg aaatgacagt atcgtgacaa taatacccag
    31801 caccgcagag atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat
    31861 tcccgccagc ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag
    31921 tgcggtgccg cgaacttgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa
    31981 gataagcgaa catccggctc cggttaacgc cgcgcccgct aatgcgaccc atgcacctgg
    32041 ggcttgccag agcagcaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt
    32101 cacgccgcca aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc
    32161 gccaaacgcg gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga
    32221 aacgaaagtc ccgataaccg caaaaccaac gccttgtagt gccagcccta accctggttt
    32281 ccagataagc ccgacaacgc tccacagcga tggacgttct cccgccaggg ccggtacttt
    32341 gcgcactgtg ccgttacagg cccacgccag taagggtaat accattgtgg tgatcgccag
    32401 tgcggcaaaa ccgtaatggc tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc
    32461 accgtaaatc gccattccgt tccatgacat cactttgcca gagtgttttg gccctacgat
    32521 gcctaacccc caggtcagag cgcctgtcag taactggctt tcaccaaacc caagaatcaa
    32581 acgcccgatg accaacaggg caaatttgaa cggtgcggag acaggcaaaa tcgccgccag
    32641 cagcaacgcg ccgccagcca gaccacaagc taacatcccc tgaagcgccg aacgttttgc
    32701 gccatattga tcggccagtc gcccggcgta accacgcgtc agcaccgtag ccagaaactg
    32761 aatcccgacg gcaatgccga ccatggtatt gccatagccc agatcatgat gaacaaacag
    32821 cgggataacc ggcaacggca accctacggt catgtaggtg agaaaaaccg caaaagagat
    32881 gcggaagagc gaaaaactgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca
    32941 ttactccaga atgcagcgca aggcgaggag tatccccgtc tcatctctct ggtttcaggg
    33001 ttacggtgcg ttggcaggat ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg
    33061 gaagtttgcc tagaactggt ggcgattgtc aatgatgtga aaaagggaac catcaggttc
    33121 ccttttgcgt tagtgccgga ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg
    33181 tcttatccga cctactccgg cctactgcga attaatcttt tcagcttgcg cattaccagc
    33241 gtggcgttgg tgccgccgaa gccgaagctg ttagacataa cagtggtcag ttcgcgatcg
    33301 gtcgtttcgg tcacgatgtt cagacccgca gcctgctcgt ccagctcttc aatgttgatg
    33361 ctcggggcga taaagccgtg ttccagcatc agcagagagt agatagcttc ctgcacaccc
    33421 gcagcgccca gtgagtgacc ggtcatggct ttggttgcag aaatcgccgg gctcttatcg
    33481 ccgaacactt cacggatagc tgccagctct ttcacgtcgc caaccggagt cgaagtaccg
    33541 tgagagttca ggtaatcgat tggggtatca acgccatgca tcgccatctg catacaacgt
    33601 actgcgcctt cgccagacgg agcaaccatg tctgcaccat cagaggttgc gccgtagcca
    33661 acgatttcag catagatgta agcaccacgc gccagcgcgt gttccagctc ttcaaccact
    33721 accataccgc cgccgccagc gataacgaaa ccgtcacggt gagcgtcgta agtacgggag
    33781 gctttttccg gggtgtcgtt gtatttagta gatagcgcac ccatcgcgtc gaattcgcag
    33841 gccatttccc agcacagctc ttcgccgccg ccagcaaaca cgatgtcctg tttgcccagt
    33901 tggatctgct ctactgcgtt accgatacag tgtgcggaag tcgcacacgc ggagctgatg
    33961 gagtagttaa cgccatgaat tttaaacggc gtggcgagac aggcagaaac gccggatgcc
    34021 atcgctttag tgaccacata cgggccaacc gctttcaggc cgcgcgggcc gcgcattgcg
    34081 tcagcgccga acacctggaa acgcggggaa ccgccgccgg aacctgcaat caggccaacg
    34141 cgcgggttat tctggtaagc ttccggagag aggcccgcat cagcgattgc ctgctccata
    34201 gaaaggaatg cataaatgga tgcgtcgctc ataaagcgca caactttgcg gtcaatgagg
    34261 ccagtggtat ccagttttat gttgccccag acgtggctac gcatgccgga atccttcagc
    34321 tcctgagaga aagtgatccc tgaacgtcct tcacgcagag atgccaggac ttcctgctgg
    34381 ttattaccga tgctggaaac aatgcccagg ccagtaatca ctgcacgttt cattcaatac
    34441 ctctgtaagt cgcacataga gtaagtttcg aatgcacaat agcgtacact tgtacgccga
    34501 acaagtccga tcagccattt aatagagata tttgcgcagc cttacacaca tcgctaagat
    34561 cgagccaccg cctgtaagac gagtaactta cgtgaaacac tactccatac aacctgccaa
    34621 cctcgaattt aatgctgagg gtacacctgt ttcccgagat tttgacgatg tctatttttc
    34681 caacgataac gggctggaag agacgcgtta tgtttttctg ggaggtaacc aattagaggt
    34741 acgctttcct gagcatccac atcctctgtt tgtgatagca gagagcggct tcggcaccgg
    34801 attaaacttc ctgacgctat ggaaggcatt tgatcagttt cgcgaagcgc atccgcaagc
    34861 gcaattacaa cgcttacatt tcattagttt tgagaaattt cccctcaccc gtgcggattt
    34921 agccttagcg catcaacact ggccggaact ggctccgtgg gcagaacaac ttcaggcgca
    34981 gtggccaatg cccttgcccg gttgccatcg tttattgctc gatgaaagcc gcgtgacgct
    35041 ggatttatgg tttggcgata ttaacgaact gaccagccaa ctggacgatt cgctaaatca
    35101 aaaagtagat gcctggtttc tggacggctt tgcgccagct aaaaacccgg atatgtggac
    35161 gcaaaatctg tttaacgcca tggcaaggtt ggcgcgtcca ggcggcacgc tggcgacatt
    35221 tacgtctgcc ggttttgtcc gccgcggttt gcaggaagcc ggattcacga tgcaaaaacg
    35281 taagggcttt gggcgcaaac gggaaatgct ttgtggggtg atggaacaga cattaccact
    35341 cccctgctcc acgccgtggt ttaatcgtac gggcagcagc aaacgggaag tagcgattat
    35401 cggcggtggt attgccagcg cgttgttgtc gctggcgtta ttacggcgcg gctggcaggt
    35461 agcgctttat tgcgcggatg aagcccccgc gctgggtgct tccggcaatc gccagggggc
    35521 gctgtatccg ttattaagca aacacgatga ggcgctaaac cgctttttct ctaatgcgtt
    35581 tacttttgct cgtcggtttt acgaccaatt acccgttaaa tttgatcatg actggtgcgg
    35641 cgtcacgcag ttaggctggg atgagaaaag ccagcataaa atcgcacaga tgttgtcaat
    35701 ggatttaccc gcagaactgg ctgtagccgt tgaggcaaat gcggttgaac aaattacggg
    35761 cgttgcgaca aattgcagcg gcattactta tccgcaaggt ggttggctgt gcccagcaga
    35821 actgacccgt aatgtgctgg aactggcgca acagcagggt ttgcagattt attatcaata
    35881 tcagttacag aatttatccc gtaaggatga ctgttggttg ttgaattttg caggagatca
    35941 gcaagcaaca cacagcgtag tggtactggc gaacgggcat caaatcagcc gtttcagcca
    36001 aacgtcgact ctcccggtgt attcggttgc cgggcaggtc agccatattc cgacaacgcc
    36061 ggaactggca gagctgaagc aggtgctgtg ctatgacggt tatctcacgc cacaaaatcc
    36121 ggcgaatcaa catcattgta ttggtgccag ttatcatcgc ggcagcgaag atacggcgta
    36181 cagtgaggac gatcagcagc agaatcgcca gcggttgatt gattgtttcc cgcaggcaca
    36241 gtgggcaaaa gaggttgatg tcagtgataa agaggcgcgc tgcggtgtgc gttgtgccac
    36301 ccgcgatcat ctgccaatgg taggcaatgt tcccgattat gaggcaacac tcgtggaata
    36361 tgcgtcgttg gcggagcaaa aagatgaggc ggtaagcgcg ccggtttttg acgatctctt
    36421 tatgtttgcg gctttaggtt ctcgcggttt gtgttctgcc ccgctgtgtg ccgagattct
    36481 ggcggcgcag atgagcgacg aaccgattcc gatggatgcc agtacgctgg cggcgttaaa
    36541 cccgaatcgg ttatgggtgc ggaaattgtt gaagggtaaa gcggttaagg cggggtaatc
    36601 tgctctcgca gtgtttgccg gatgcggcgt gaacgcctta tccaacctac gtgtgacgtg
    36661 taggcctgat aagacgcgat aagcgtcgca tcaggcatgg tgctccagac gccgcaactt
    36721 acagctgtga cgcctgctga aacaggtttt cccacatatc ggtcaccagc gcctggtcac
    36781 gcggcgacaa ttcaccggca ccaatggctt tttccagact ctggctaacg gtcgtatgca
    36841 ccgcctgagc ggagtggtca tcaccacttt ccagttctgc gatggctaat gtcaggtggc
    36901 cacgcaaata cccactggca aacaactcat catcactggc atggtcaacc atgccgtcga
    36961 ttaatgccag aatgcgtgat tcaaactccg cgatcatctt ctttcctcat tgtaaaacgt
    37021 ggcgctggct tcagttgagc gcttccggcc acgggaactg ttccgccgta agttccggcg
    37081 tgtgataata attttgtagt gctttaatga agcgtgccgg gcgttccgga atgccatttg
    37141 tcagataatc catcacctgc gcatgtaccc gccgttgaaa caccacacga tccggttcga
    37201 aatccccttc cagattgtcg caactgacgt taaacggata tccagccgcc acgcagaata
    37261 accagtcgaa cgcctgcggc ttcacttcaa catcttcaaa ctggctttgc gtctgggcat
    37321 cgcgtccgtc cgggcaatac cagtagccga aatcaaccag ctcacggcgc gcttttccgg
    37381 caatacacca gtgcgaaatc tcatgaattg cgctggcata aaagccatga gcaaagacga
    37441 ttcggttata cggtacttcc gcatcagcag gaagatagat cggttcgtcg tcgcctttaa
    37501 tcagacgggt attaaattca tcggcaaagc agctattaaa aatttcaatc aactgctcgt
    37561 agtggtgtgt actgttcatt agttcatccc caaccagtgg aggatctcct gtccgtggct
    37621 atcgtaaagt aatttggcac tcatcaccgc cgagacgata acaatcatcg ggcggatcag
    37681 cttttgtcct ttgctcaaca ccaaccgtga acccatgcgc gcgccgagga actgccccac
    37741 cagcatgaca aagcccgtcg cccaaatcac tttgccgccg agaataaaca gcagcagacc
    37801 gccaatgttt gacgttgcgt tgagtaattt ggcgtgagcc gtggctttgg cgaggttgaa
    37861 tccgcacagc ataacgaagg ccagcgcgta aaacgatccg gcagccgggc caaagaatcc
    37921 gtcataaaaa ccgacgcagc caccggcaat taacgcgaag ggtaaaccgt acatccggcg
    37981 ctggcgatct tcttcaccca gctttggcat cagcaagaaa tagaggccga tacaaatcac
    38041 cagaatgggc aaaatctgcc gtaagacatc agcctgaacg tactgtacca acagtgcgcc
    38101 gctcattgag ccgacaaagg tcatggcaat attgagtttc tgatcgctta aactaaccac
    38161 tttgcggcga ataaagtaga tagtggcgga aatagagccg ccgcaggctt gcagtttatt
    38221 ggttgccagc gcgttagcgg gagacattcc cgcagccatc aatgccggaa tggtgagtaa
    38281 cccaccgcca ccggcaatcg agtcgataaa tcccgccagc atggcgacaa aaaagagaac
    38341 tcccagcaac agcggagaaa ccataaacag gctattaaac gtttccatta gatcacgtgc
    38401 tcatccagta gcgcctggca ggaaggcggc aacggaggcg gtgtcttctt ctcaggcttt
    38461 gttgttcccg gttttggagg ttcaaaccag ctttgcagtt ctgccccgca accatcgcct
    38521 gatggcggta aaggttgatc ttcacactcc agactatcgg cagggcaacg taatcgtaca
    38581 tgcatatgcg cgcgatgctg gaaccagggt cgcactttgc gcaaccagtc gcgatcggtg
    38641 cccgcatcaa ggcaaagttg ttgtttaatc gccggattaa caaaaatgcg cgtgacgtct
    38701 ttgtcctggg cggcgagttt gatcaagctg aaaatttctg gcttccacag cgtggagaca
    38761 acgtgtttac cgtcgcggga aactaagtcc agtgcttgcg ggcgcaagag ctgcgcggag
    38821 gtccagcgag ttttcggcag ttgcagaaag atatcgacat ccagtccggt ctggtggctg
    38881 gcatgaccgc cgttgaaacg cccaccagcg ggcatcccca tatcgccaat cagcaccgta
    38941 cccatgccca gattgctcac ctggctactc agacgctgga taaacatcac cagatccggg
    39001 tgaccgaaat agcgacgctg atcggtacgc atgacctgat aatgttcgga ctgtatcggc
    39061 aacgtgtcag cgccgacaat acagccatta gaaaaactgc ctatcgattg tgcgctaccc
    39121 ggcacaggtt gggttatttt ttgccacggc gtcgctgcca ggctagcact actggcaagc
    39181 agagccagca gcgcaatcgc ggttttgttc attttttacc agcgtggaat atcagtcttc
    39241 acatcggcat tttgcgcccg ctgccgtaac aggtgatcca ttaaaacgat cgccagcatc
    39301 gcttcagcga tcggcactgc acggatcccg acacagggat cgtgacggcc tttggtgatc
    39361 atctcaactt cttcgccaaa gcggttaata gtgcgccccg gcacggtaat gctggaggtc
    39421 ggtttcagcg ccatatgagc aatgatttgc tgcccgctgc tgataccgcc gaaaatgccg
    39481 cccgcatggt tgctctggaa accgtctttg gtgatttcgt cgcggttctg gctgccgcgc
    39541 agcgccacta cgtcaaaacc gtcgccaatt tccacgcctt tcaccgcgtt gatgctcatc
    39601 agcgcatggg cgatgtcggc atccaggcgg tcaaagaccg gctcgccaag tccggcgggg
    39661 acaccactgg caacaacggt gactttcgcg ccgatggagt cgccctcttt t
//
LOCUS       DAZXUK010000009        36996 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZXUK010000009 DAZXUK010000000
VERSION     DAZXUK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36996
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.009"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..74)
                     /locus_tag="ACH3L8_000398"
     CDS             complement(<1..74)
                     /locus_tag="ACH3L8_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HFT4083673.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="ACH3L8_000399"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="ACH3L8_000399"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFT4083674.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="ACH3L8_000400"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="ACH3L8_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFT4083675.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="ACH3L8_000401"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="ACH3L8_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFT4083676.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="ACH3L8_000402"
     CDS             2704..2940
                     /locus_tag="ACH3L8_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFT4083677.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="ACH3L8_000403"
     CDS             2943..3800
                     /locus_tag="ACH3L8_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFT4083678.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5812
                     /locus_tag="ACH3L8_000404"
                     /pseudo
     CDS             3800..5812
                     /locus_tag="ACH3L8_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="ACH3L8_000405"
     CDS             complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="ACH3L8_000405"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFT4083679.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6415..7311)
                     /locus_tag="ACH3L8_000406"
     CDS             complement(6415..7311)
                     /locus_tag="ACH3L8_000406"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFT4083680.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7360..7599)
                     /locus_tag="ACH3L8_000407"
     CDS             complement(7360..7599)
                     /locus_tag="ACH3L8_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFT4083681.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7702..8301
                     /gene="nemR"
                     /locus_tag="ACH3L8_000408"
     CDS             7702..8301
                     /gene="nemR"
                     /locus_tag="ACH3L8_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFT4083682.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8338..9435
                     /gene="nemA"
                     /locus_tag="ACH3L8_000409"
     CDS             8338..9435
                     /gene="nemA"
                     /locus_tag="ACH3L8_000409"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFT4083683.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9516..9923
                     /gene="gloA"
                     /locus_tag="ACH3L8_000410"
     CDS             9516..9923
                     /gene="gloA"
                     /locus_tag="ACH3L8_000410"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFT4083684.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10026..10673
                     /gene="rnt"
                     /locus_tag="ACH3L8_000411"
     CDS             10026..10673
                     /gene="rnt"
                     /locus_tag="ACH3L8_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFT4083685.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10766..15224
                     /locus_tag="ACH3L8_000412"
                     /pseudo
     CDS             10766..15224
                     /locus_tag="ACH3L8_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15268..16476)
                     /locus_tag="ACH3L8_000413"
     CDS             complement(15268..16476)
                     /locus_tag="ACH3L8_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFT4083686.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16499..16909
                     /gene="tnpA"
                     /locus_tag="ACH3L8_000414"
                     /pseudo
     CDS             16499..16909
                     /gene="tnpA"
                     /locus_tag="ACH3L8_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="ACH3L8_000415"
     CDS             complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="ACH3L8_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFT4083687.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17705..18532
                     /gene="mepH"
                     /locus_tag="ACH3L8_000416"
     CDS             17705..18532
                     /gene="mepH"
                     /locus_tag="ACH3L8_000416"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFT4083688.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18660..19241
                     /gene="sodB"
                     /locus_tag="ACH3L8_000417"
     CDS             18660..19241
                     /gene="sodB"
                     /locus_tag="ACH3L8_000417"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFT4083689.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19387..20556)
                     /locus_tag="ACH3L8_000418"
     CDS             complement(19387..20556)
                     /locus_tag="ACH3L8_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4083690.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20722..20811)
                     /gene="cydH"
                     /locus_tag="ACH3L8_000419"
                     /gene_synonym="ynhF"
     CDS             complement(20722..20811)
                     /gene="cydH"
                     /locus_tag="ACH3L8_000419"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFT4083691.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            21110..22135
                     /gene="purR"
                     /locus_tag="ACH3L8_000420"
     CDS             21110..22135
                     /gene="purR"
                     /locus_tag="ACH3L8_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFT4083692.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22132..23064)
                     /gene="punR"
                     /locus_tag="ACH3L8_000421"
     CDS             complement(22132..23064)
                     /gene="punR"
                     /locus_tag="ACH3L8_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFT4083693.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            23177..24388
                     /gene="punC"
                     /locus_tag="ACH3L8_000422"
     CDS             23177..24388
                     /gene="punC"
                     /locus_tag="ACH3L8_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFT4083694.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24679..25827
                     /gene="cfa"
                     /locus_tag="ACH3L8_000423"
     CDS             24679..25827
                     /gene="cfa"
                     /locus_tag="ACH3L8_000423"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFT4083695.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25867..26508)
                     /gene="ribE"
                     /locus_tag="ACH3L8_000424"
     CDS             complement(25867..26508)
                     /gene="ribE"
                     /locus_tag="ACH3L8_000424"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFT4083696.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26723..28096
                     /gene="mdtK"
                     /locus_tag="ACH3L8_000425"
     CDS             26723..28096
                     /gene="mdtK"
                     /locus_tag="ACH3L8_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFT4083697.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28137..29393)
                     /locus_tag="ACH3L8_000426"
     CDS             complement(28137..29393)
                     /locus_tag="ACH3L8_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFT4083698.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29701..29777
                     /locus_tag="ACH3L8_000427"
     tRNA            29701..29777
                     /locus_tag="ACH3L8_000427"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29735..29737,aa:Val,seq:gac)
     gene            29782..29858
                     /locus_tag="ACH3L8_000428"
     tRNA            29782..29858
                     /locus_tag="ACH3L8_000428"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29816..29818,aa:Val,seq:gac)
     gene            29966..30271
                     /locus_tag="ACH3L8_000429"
     CDS             29966..30271
                     /locus_tag="ACH3L8_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFT4083699.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30397..31819
                     /locus_tag="ACH3L8_000430"
                     /pseudo
     CDS             30397..31819
                     /locus_tag="ACH3L8_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(31831..32643)
                     /gene="ydhT"
                     /locus_tag="ACH3L8_000431"
     CDS             complement(31831..32643)
                     /gene="ydhT"
                     /locus_tag="ACH3L8_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFT4083700.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32647..33432)
                     /gene="phsC"
                     /locus_tag="ACH3L8_000432"
     CDS             complement(32647..33432)
                     /gene="phsC"
                     /locus_tag="ACH3L8_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFT4083701.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33429..34097)
                     /locus_tag="ACH3L8_000433"
     CDS             complement(33429..34097)
                     /locus_tag="ACH3L8_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFT4083702.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34161..34799)
                     /locus_tag="ACH3L8_000434"
     CDS             complement(34161..34799)
                     /locus_tag="ACH3L8_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFT4083703.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34812..36913)
                     /locus_tag="ACH3L8_000435"
                     /pseudo
     CDS             complement(34812..36913)
                     /locus_tag="ACH3L8_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(36934..>36996)
                     /locus_tag="ACH3L8_000436"
     CDS             complement(36934..>36996)
                     /locus_tag="ACH3L8_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4083704.1"
                     /translation="CANACPTGALKIIEWKDITV"
BASE COUNT         8853 a         9693 c         9128 g         9322 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccagct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgtaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg cagtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttactttcgc tattcagctt tgtggtcaaa tttggcctga tggtccagat
     5101 tagcgatctg tggcaatttt tactgtttct ctttccactg ctggcgacaa tgcagcttct
     5161 taaattgcag atgcccaaat ttgccgcact gtgggggcaa ctgattgttt ttatgggttc
     5221 gtttatcgct gtcactaatc ccccggtgta tgattttgct gattttctta actataatct
     5281 ggcaaaaatc gttggcgttg cgctggcttg gctagcgttc gccattctgc gtccaggatc
     5341 ggatgctcgt aaaagccgcc gccatattcg cgcgctgcgc cgggattttg tcgatcagct
     5401 aagccgccat ccaacactga gtgaaagcga atttgaatcg ctcacttatc atcacgtcag
     5461 tcagttgagt aacagccagg atgcgctggc tcgccgttgg ttattacgct ggggtgtagt
     5521 gctgctgaac tgttctcatg ttgtctggca attgcgcgac tgggaatcgc gttccgatcc
     5581 gttatcgcga gtacgggata actgtatttc actgttgcgg ggagtgatga gtgagcgtgg
     5641 cgttcagcaa aaatcactgg cggccacact tgaagaatta cagcggattt gcgacagcct
     5701 tgcccgtcat catcaacctg ccgcccgtga gctggcggca attgtctggc ggctgtactg
     5761 ctcgctttcg caacttgagc aagcaccacc gcaaggtacg ctggcctcgt aattacttaa
     5821 ttacaccaca ggcatagcgt tcaccgccac cgcccagcgg tttaggttga tcggacatat
     5881 tatcgccgcc aacgtggacc atcagcgcct tgtctttgat ttcatccagt gatttcagac
     5941 gaggcgcgat gacggcatcg gtagctttgc cgtcattatt gacgaccagt gcaggcagat
     6001 cgcctaaatg cccggtacct tctggccctt catgtttacc ggtattttgt ggatcaagat
     6061 gcccgcctgc ggattccgcg gcgctggctt tgccatcttt ggtggctggc tggcagcttc
     6121 ctttggcatg aatatggaag ccatgttcac cggggggtaa tgctttcaga tcgggtgaaa
     6181 actccagacc tttatcggtt tcagtaatgg tgacgctacc aattgactgc ccaacccctt
     6241 gcgacgtgac gaggttcatc tcgacttttt cactggcagc ttgtgcgccg gtcgcaacaa
     6301 ccagcgccag aatggccaga ctaaaacgtt tcataggacc tccgttcatt gtgcattcct
     6361 gaataagtgt aaaccagtga cacatttttg aacggcggct attcctaaaa gtgcttacgg
     6421 tacgtcgtac cccagtgcag ctttacggat acgaaaccat tgttgacggg tcattttcag
     6481 tgtttctgct tcgacagctg cccgtacgcg ctcaatttta cctgagccga taattggcag
     6541 cggctgcgac ggtaaacgta atacccaggc gtaaaccacc tgttcaatcg agcccgcgtt
     6601 taactcctcc gccaccacag ccagttcatc acgcagcggc tggaaataat catcattaaa
     6661 cagacgacca ccaccaaggc aggaccacgc catcggacga acacgcagtt gttgtagttg
     6721 gtcgagcgtg ccatccagca gtaacggctg atgcaccggg gatatttcca cctgattagt
     6781 ggcaagggta aacggcagac gtgattgcaa cagggcaaat tgcgcaggcg taaagttcga
     6841 tacgccaaaa tgacgcactt tgccgctctg atgcagatgt ttgaacgcgt ccgccacttc
     6901 atcggcatcc attaacgggt ctggtcggtg gattaacagc agatccagat gatcggtcgc
     6961 gagattaatt agcgactgtt cggcgctctt aatgatgtga ttgcggtcag tgatgtaatg
     7021 accaatgacg ttttcttcac gcgcggtcgt cgcgataccg catttactga cgatttccat
     7081 ccgttcacgc aggtgaggtg ccagtttcag tgcctcgcca aacgccgctt cgcactgata
     7141 gccaccataa atatcagcat ggtccacggt ggtcacgccg agatccagat gctcttcaat
     7201 aaaactgacc agctggcggg cggacatatt ccagtccatc aatcgccagt agcccatcac
     7261 aaaacgggaa aactccgggc cttccggcgc aatagtaata cgctgaacca taatcgcttc
     7321 ctcttatcag atataagagg agtatacgca agattaggtt caaaagagtg atggttgctc
     7381 cggttcgtct gatgacgctg gcttatttgc gcgtaatttg cgcattaatc gctgccgaca
     7441 aaggcgcagc acctcttgtt tttcgccatc gctcatttta ttccagttaa aacgctcatc
     7501 ccgactacga aaacagccgc gacaaaaccc gcgttcgtca gactggcaaa ttccccggca
     7561 cgggctctgg acgggaaaga actctaattg ctccgccacc tcgccctcct cagataagat
     7621 tattaccatt attgaagctg ttaatgtcca aagtagcaac tttgcttgca ctagaccaac
     7681 tggtctacta cactccaacg catgaacaaa cacaccgaac atgatactcg cgaacatctc
     7741 ttggcgacgg gcgagcaact ttgcctgcaa cgtggattca ccgggatggg gctaagcgaa
     7801 ttactaaaaa ccgctgaagt gccgaaaggg tccttctatc actactttcg ctctaaagaa
     7861 gcgtttggcg ttgccatgct tgagcgtcat tacgccgcat atcaccagcg actggctgag
     7921 ttgctgcaat ccggcgaagg taactaccgc gaccgcatac tggcttatta ccagcaaaca
     7981 ctgaaccagt tttgccaaca tggaaccatc agtggttgcc tgacagtaaa actctctgcc
     8041 gaagtgtgcg atctgtcaga agatatgcgc agcgcgatgg ataaaggtgc tcgcggcgtg
     8101 atcgccctgc tctcgcaggc tctggaaaat ggccgtgaga accattgttt aaccttttgt
     8161 ggcgaaccgc tacaacaggc acaagtgctt tacgcactat ggcttggcgc gaatctgcag
     8221 gccaaaattt cgcgcagttt cgagccactg gaaaacgcgc tggcccatgt aaaaaacatt
     8281 attgcgacgc ctgccgttta gcaggcattt tttatcacca gacgaccggg agcctttatg
     8341 tcatctgaaa aactgtattc cccactgaaa gtgggcgcga tcacggcggc aaaccgtatt
     8401 tttatggcac cgctgacgcg tctgcgcagt attgaaccgg gtgacattcc taccccgttg
     8461 atggcggaat actatcgcca acgtgccagt gccggtttga ttattagtga agccacgcaa
     8521 atttctgccc aggcaaaagg atatgcaggt gcgcctggca tccatagtcc ggagcaaatt
     8581 gccgcatgga aaaaaatcac cgctggcgtt catgctgaaa atggtcatat ggccttgcag
     8641 ctgtggcaca ccggacgcat ttctcacgcc agcctgcaac ctggcggtca ggcaccggta
     8701 gcgccttcag cacttagcgc gggaacacgt acttctctgc gcgatgaaaa tggtcaggcg
     8761 atccgcgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc gggtatcgtc
     8821 aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg cttttgatct ggtagagctc
     8881 cactctgctc acggttattt gctgcatcag ttcctttccc cttcttcaaa ccatcgtacc
     8941 gatcagtacg gcggcagcgt ggaaaatcgc gcacgtttgg tactggaagt ggtcgatgcc
     9001 gggattgaag aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat cggtactttc
     9061 cagaacacag ataacggccc gaatgaagaa gccgatgcac tgtatctgat tgaacaactg
     9121 ggtaaacgcg gcattgctta tctgcatatg tcagaaccag attgggcggg gggtgaaccg
     9181 tatactgatg cgttccgcga aaaagtacgc gcccgtttcc acggtccgat tatcggcgca
     9241 ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat tgatgcggtg
     9301 gcatttggtc gtgactggat tgcgaacccg gatctggtcg cccgcttgca gctcaaagct
     9361 gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg gcgcggaagg ctataccgat
     9421 tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc cgccgctata ctaaaacaac
     9481 attttgaatc tgttagccat tttgaggata aaaagatgcg tcttcttcat accatgctgc
     9541 gcgttggcga tttgcaacgc tccatcgatt tttataccaa agtgctgggc atgaaactgc
     9601 tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc tacggcccgg
     9661 aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa tacgaactcg
     9721 gcactgctta tggtcacatc gcgcttagcg tagataacgc cgctgaagcg tgcgaaaaaa
     9781 tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc ggtactacgg
     9841 ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa gagaaagacg
     9901 ccggtcgcgg tctaggcaac taatctcctg ccgggcgtga actcatcgcg cccgcatctt
     9961 tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg tattaagaga
    10021 atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt ggtttttatc
    10081 ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg ctgcttgaga
    10141 ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac accacattac
    10201 atttccacgt cgaaccattt gtcggcgcaa atttgcaacc agaagccctc gccttcaacg
    10261 gcattgaccc gaacgatccc gatcgcggcg cggtcagcga atacgaggcg ctgcacgaaa
    10321 tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc attatggtgg
    10381 cgcacaacgc caattttgat cacagcttta tgatggccgc cgcagaacgc gcctcgctga
    10441 aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc gggctggcac
    10501 tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc gacagcaccc
    10561 aggcgcactc cgcgctgtac gacaccgaac gcactgctgt gctgttttgt gaaatcgtca
    10621 accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg taatcgagtc
    10681 gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca cgcatcaagc
    10741 atgatattca cggggaatag cgttaatggc agataatcca gacccttcat cgctcctgcc
    10801 ggacgtgttt tcaccggcga cccgcgactg gtttcttcgc gcctttaaac agccgaccgc
    10861 tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa catgcgctgg tgattgcacc
    10921 gactggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc tcttccgcga
    10981 aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc tctatatttc
    11041 accgataaaa gccctgggca ccgacgttca gcgcaacttg cagatcccgt tgaagggtat
    11101 tgccgatgaa cggcggcggc gcggcgaaac ggtaattatt gatgaagtcc acgcggtagc
    11161 gggcagtaaa cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca
    11221 cacctcagca cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc
    11281 atttcttggt ggcgatcgcc cggttacggt agtcaacccg cccacaatgc gccatccgca
    11341 gatacgaatt gtcgtaccgg tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac
    11401 cggcgaagac agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat
    11461 ccttgatgaa gtgttgcgcc atcgctcgac cattgtcttt actaattcac gcgggctggc
    11521 ggaaaaactg acggcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc
    11581 tatcgccgtt gatgcggccc atttcgagtc gacctccggc gcaacctcta accgtgtaca
    11641 aagtagcgac gtttttattg cccgctcaca ccacggctcc gtctctaaag aacaacgagc
    11701 aatcaccgaa caggcgctga aatcgggtga attacgctgc gtggtcgcaa cctccagtct
    11761 tgaactgggg attgatatgg gcgcggtgga tctggtgatt caggtggcaa cgccgctttc
    11821 tgttgccagt gggttacaac gcattggtcg cgccggacat caggttggcg gtgtatctaa
    11881 agggctgttt ttcccccgta cccggcgtga tttagtcgat tccgcagtca ttgtagagtg
    11941 tatgttcgca ggcaggctgg aaaacctgac accaccgcat aatcctctcg acgtccttgc
    12001 gcagcaaacc gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg
    12061 cgtacgccgt accgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga
    12121 tatgctttcc gggcgctatc cctctggcga tttttctgct tttcgcccca aactggtctg
    12181 gaacagggag accgggatat tgaccgcccg acctggcgct caattgttgg cggttaccag
    12241 cggcggcacc attccggatc gtggcatgta tagcgtgtta ttacccgaag gtgaagaaaa
    12301 ggccggttcg cggcgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa
    12361 cgacattatc actctcggcg ctacctcatg gcggatccag caaatcaccc gcgatcaggt
    12421 gattgtgact cctgctccgg gtcgttctgc ccagctcccc ttctggcgtg gtgaaggtaa
    12481 cggacgtccg gctgaattag gcgagatgat cggcgatttt cttcatttac tggcggatgg
    12541 cgcgttcttt tccgggacta ttcccccgtg gctggcagaa gaaaatacga acgccaatat
    12601 tcaggggttg attgacgagc aacgcaacgc gacgggcatc gttccgggga gtcgccatct
    12661 ggtcctcgaa cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta
    12721 tggaagacgg gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgccctatg
    12781 gggcgctgac gcgtctgtgg tcgccagtga tgacagcatt gttgcgcgca ttcctgacac
    12841 tgatggtaaa ttgcccgacg ccgcgatttt tttgttttaa ccagaaaagt tgctgcaaat
    12901 tgtccgcgag gcggtaggca gctcggcact tttcgccgcc cgttttcgcg aatgcgccgc
    12961 gcgggcatta ttaatgccgg gccgcacgcc gggccatcgc accccgcttt ggcaacaacg
    13021 gctgcgcgcc agtcagttgc tggaaatcgc tcagggatat ccggattttc cggtcattct
    13081 cgaaacccta cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat
    13141 gcgtcgcctg aacggtggcg aaattcaaat atccgatgta acgaccacta cgccctcgcc
    13201 tttcgccaca agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc
    13261 gctggcagag cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact
    13321 cggacaggtc gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga
    13381 gttgcaacga ctggctcctg gtagaagagc gaaaggtgaa gaaggattgt tcgacctgct
    13441 gcgcgaactg gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga
    13501 agaggttgcg tcgtatctgg aaaatcttct tgcagtaaaa cgaatcttcc cagcgatgat
    13561 tagcggacag gagcgtcttg cctgtatgga tgatgccgcc aggctgcgtg atgccctcgg
    13621 cgtacggcta ccagagtcat tgccagagat ttatttacat agagtcagtt acccgcttcg
    13681 cgacctcttt ctgcgctatc tccgggctca tgctctggtc acggctgaac aactggctca
    13741 tgagtttagt ctcggtattg ccattgtcga agagcagctt cagcaactgc gtgaacaggg
    13801 tctggtgatg aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg
    13861 tttgcgctcg ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc
    13921 gcgattgctg ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt
    13981 tgcctcaaca tctccaggcg tttatgaggg cgtagatgga gtgatgcggg tgatcgaaca
    14041 gcttgccgga gtcggtttac ccgcctcact ctgggaaagc cagatcctgc ctgcccgcgt
    14101 acgcgactat tcgccagaaa tgctcgatga attactggca accggagcgg ttatctggtc
    14161 gggacaaaaa aaagctgggt gaagatgacg gcctggtggc actgcatcta cagaaatatg
    14221 ccgcagaatc gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg ctgcagcaag
    14281 cgataatcgc tgttctggct gacggaggag cctggtttgc acaacaaatc agccaacgga
    14341 tacgcgataa aatcgtcgaa tcggttgatt cctcggcact gcaagaggcg ttatgggcgc
    14401 tggtctggca aggcgtcatt accagcgata tttgggcacc gttacgcgcc ctcacccgca
    14461 gcagttccaa cgcacgcacc tcaactcgcc gcagtcaccg ggctcgtcgt gggcgtcctg
    14521 tctatgcaca acccgtctcg ccgcgggtat cttacaacac accaaatctg gctggacgct
    14581 ggtcgttatt gcaggtggag ccactaaacg ataccgaaag gatgctggcg ctggcggaaa
    14641 atatgctcga ccgctacggc atcatcagtc gtcaggcggt gatagccgaa aatatccctg
    14701 gcgggtttcc atcgatgcaa acactttgtc gaagtatgga agactccggg cgaattatgc
    14761 gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg actattgacc
    14821 gattgcgcga tctggcgaca caagccacgc aaacacgcca ctatacacca gtggcgctct
    14881 ccgccaacga tccggctaat gtgtggggaa atcttctgcc ctgggctgca catccggcaa
    14941 cgctggtacc aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc aaattgttac
    15001 tctatctggc gcaaggcggc aaaaaaatgc tggtctggca ggaaaaggag gaattactcg
    15061 ccccagagat tttccaggcg ctgaccaccg cactgcgtcg cgaaccaagg ctgcgcttta
    15121 cgctaacaga agtgaatgat cttccggtcc ggcaaacgcc gatgtttacg ctgctgcgtg
    15181 aggcgggatt ttcaagttcg ccacaagggc tggattgggg atagttgaca tcctccacgc
    15241 cctgaaagac gtggttccct gctacgttca agctgtcgcc tgaatcattt cggtgggttc
    15301 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    15361 cgctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac atacctggca
    15421 tctgaattta ctttgtgaca ggcggttttc tttcgctgta tgaccacagc acgcgcaacg
    15481 ctggcttgtg tacgctggcg gaacagcaag cacctgaccg ccactccaga gctgcttgta
    15541 ctcaagctgg cggcgcattt cataccagcc ctgatccagt atcgaacggt ttaaacctga
    15601 ttttgcccgg acattgcgcc ccggctggct gaccgtaccc gctgctgact ttgacatgtt
    15661 gctgaccttc aaatcctcaa tgactatcat tgcgtggttt ttgctgacgg tcgttgtgac
    15721 tttgtgaagg tagtccctgc ggatatttgc gatacaggaa tgcagtcgct gtattttgcg
    15781 tttctgcttc tgccagttgt tgctgaattt gaccttccgg cttaactgtc gctgaagtct
    15841 cgccagcgtc ttctggtttt tttgaaaact gtttacaggc tcaaagactg tgccatctga
    15901 cagcgtggcg agtttagcca cgccagcatc cagcccgatc attgatgctg aagggtgaac
    15961 cggagttgat acttcacttt ctgtctgaat actgatgtac cacgtaccgc aggactggct
    16021 gacagtgaca tttttcacaa ctcccgtgac ctgacggcta ttccggtagc gcatccagcc
    16081 aagtttcggc agaaaaatac ggctgttttc ctgatcgagc ttaacgtcct gcgggtagcg
    16141 gaatgcagca ttctgtcccc gctttttgaa tcggggaaaa gcagcccgct tctggaagaa
    16201 gtttttgtaa gcccgctcaa ggtctttcag tgactgttgc aatggctgtg agggcgaatc
    16261 tttaagccat tgcgtttcag tggcgttttt ccactcaacc agccaggaag ccattttccc
    16321 gtaagggatg tatttattac cggcctcatg attctcattc tgaagcgcca gagcacgatt
    16381 gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca cgctcctgtt gaccaccggg
    16441 tcttaactgg aatttaaatg cctgtaatcg tttcacaggt caaatgatac tttggtctgt
    16501 gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt ttcctgatgc atgttcacct
    16561 ggtctttgtc accaaatacc gacgccagat ttttgaccat gacacgacag aaaaactacg
    16621 cacttacttt tcaaatgtat gtgctgattt tgcagctgaa ctggttgaaa tggatggcga
    16681 accagatcac gtccatttgt taattaacta tcctcccaaa ctggcgatat ccagtctggt
    16741 aaacagcctc aaaggcatat cgaataggtt actgcgacga gatcgaccag atattgcagt
    16801 caggtattac tacaaaggcg ttttgtggag tcctggctat tttgccagta gctgcgaagg
    16861 tgcgccaata tccgtcatcc gccagtacat tgaacaacag caaacacctg gtcaggtgga
    16921 aaaccgcgcc ttatatcccc gccctgaagg acgaggtttt acggcgcacc ggataaagga
    16981 ctgacggatg cccgttcgca tccgtcagta ttgcaggacg gattattccg cgtccggctc
    17041 ttcagacttg tatttagcgg cagtttcttt gatcagctgc tgcagttcgc cacgctgata
    17101 catttcgatc acgatatcac aaccgccgac cagctcgccg tcaacccaca gttgcgggaa
    17161 ggtcggccag ttagcatatt tcggcagttc cgcacgaatg tccggattct gcagaatatc
    17221 aacataggca aaacgttcgc cacatgcggc aagcgcctgg actgcctggg cagagaaacc
    17281 gcagctcggc agtttcggtg aacctttcat gtacagcagg atcgggtttt cagcaatctg
    17341 gcgttggatt ttttcgatag tggtgctcat tgtcttgctt cctcaaactt ctttacggca
    17401 gtaatctgac attgtagcgg gtcagtgcgg catcggaaaa taacattttc atcacgcttt
    17461 tgctatttta tccctttgct ctatttgttg catttcaaat attcgtttct tctctgatgc
    17521 gcggcaaact ggcgtattta taaccatttt tagctttcac tgctgcattt ttttgcgctc
    17581 gccaacgaaa cgtatttttt aacaataaaa gctattaact ttctcttctt ctatgcatta
    17641 gaatcatcaa gttttgtaaa tcagacgcag gcatgataga cctgccttaa cagagggacg
    17701 ctcagtggcg cggataaacc gtatttcgat cacgctctgt gctttacttt ttaccaccct
    17761 gcctttaacg cctatggccc atgcttcaaa gcaagccagg gagagttctg ctaccactca
    17821 tatcaccaaa aaagcagata aaaagaaaag cacggcaacc accaaaaaga cccagaaagc
    17881 cagccagaaa acagcgaaaa aagccgccag taaaagtatg accaaaagca aaaccgcttc
    17941 ttctgttaaa aaatcttcca ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc
    18001 cgtcaataaa acggcctcag ccagctttac cgaaaagtgt accaaacgta agggctataa
    18061 atcgcattgt gtgaaagtca aaaatgccgc gtcaggaacc cttgccgacg cgcacaaagc
    18121 gaaggtgcaa aaagccacga aagtggcaat gaataaactg atgcagcaaa ttggtaagcc
    18181 atatcgttgg ggcggcagct caccgcgtac cggttttgat tgcagcggcc tggtttatta
    18241 cgcttataaa gatttggtga aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct
    18301 gcgtgatgca ggacctatcg aacgtagtga gctgaaaaac ggcgacctgg tctttttccg
    18361 tactcaggga cgcggtacag ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat
    18421 tcagtcaccg cgcacaggtc aggaaattca aatcacttct ctcagtgaag actactggca
    18481 gcgccactat gttggcgctc gtcgggtaat gaccccaaaa acacttcgct aaaactttac
    18541 cctgttgtta cggcaacagg gtaagttcat cttttgtctc accttttaat ttgctaccct
    18601 atccatacgc acaataaggc tattgtacgt atgcaaatta ataataaagg agagtagcaa
    18661 tgtcattcga attacctgca ctaccatatg ctaaagatgc tctggcaccg cacatttctg
    18721 cggaaactat cgagtatcac tacggcaagc atcatcagac ttatgtcact aacctgaaca
    18781 acctgattaa aggtaccgcg tttgaaggta aatcactgga agagattatt cgcagctctg
    18841 aaggtggcgt attcaacaac gcagctcagg tctggaacca tactttctac tggaactgcc
    18901 tggcaccgaa cgccggtggc gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt
    18961 ttggcagctt tgccgatttc aaagcgcagt ttactgatgc agcgatcaaa aactttggtt
    19021 ctggctggac ctggctggtg aaaaacagcg atggcaaact ggctatcgtt tcaacctcta
    19081 acgcgggtac tccgctgacc accgatgcga ctccgctgct gaccgttgat gtctgggaac
    19141 acgcttatta catcgactat cgcaatgcac gtcctggcta tctggagcac ttctgggcgc
    19201 tggtgaactg ggaattcgta gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag
    19261 cattgcctga tgcgcttcgc ttaccatgcc aacgcggatg aggcgttaac agcgcattcg
    19321 gcaagataaa tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt
    19381 gtttaattag ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac
    19441 cagcaataat gccagtcccg cgacaatcgc ccccgtcacc ggcacaaagc tgtatcccag
    19501 cccagcggaa attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc
    19561 accaatattg actgatgaag acagacctgg cgcttcactg gcgacatgca tcacgcgcat
    19621 ctgtaacgga ggtacaaccg caaaggttgc tgcgccccac accaccatgc taatagctgc
    19681 gccgaactca ttgcgggcca ggaacgggat tgccagcata atcaccatca gcagcaacaa
    19741 aaagcctttc aacgtgccgt taactgaacg atctgccagt ttgccgccga gatagttacc
    19801 gatagagaat ccgacaccaa tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg
    19861 ggtaatactt tgcagtaccg gagagatata ggtgtagagg gtaaacattg caccagctcc
    19921 cagtaccgtc gtcagcaatg cagacagcac ctgcggacgc attaataccg ccagctcttt
    19981 tttcacttca ggtcgtgccc ctgtaccacc tttaggtaat gagaagaaca gacttaccat
    20041 tgaaatcact cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc
    20101 caaccaggtc gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt
    20161 ggcaactgcg ctggcctgtt tatgttttgg caccacgctt gcggccacga ctgaacccaa
    20221 accaaaaaat gctccgtgat tcaggctggt caaaatacgt gaaagcatca gggtcatata
    20281 atccggcgcg atggcggaaa gaacgttgcc tagcgtgaaa attgccatca ggaaaatcaa
    20341 cgcactgcgg cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc ccaccattac
    20401 gccaactgca taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg
    20461 cgcaatgacg ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc
    20521 aatcgccagc gccagcaacg gatagttaat tttcatgcct tatctccacc tcttcgcgtc
    20581 attacgcgat attcattaaa gtggcgaaag catgacagca atcacaaaaa aatgaaaata
    20641 acaaaaagag aaaacacttt tgccattttg ctaacaaaca ggaaggagat gcgagggaga
    20701 acgcgctccc tcgagaggaa atcagtgcag cgcggcagtc aaacccacgg ctacgatcaa
    20761 accgaggacg ataaccgttg ttaccagtga aaatttaagg tcggtgctca tcaagttttc
    20821 tcctttttta ttaccacaca aaaagtgata ttacgcattt ttacacactg tgatgaaaaa
    20881 atctcccgtc atttataatg ataagtgttt ttaccacttc cccttttcgt caagatcggc
    20941 caaaattcca cgcttacact atttgcgtac tggccattga ccccttcctg acgctccgtg
    21001 tcgtttttcc ggcgtaccgc aacacttttg ttgtgcgtaa ggtgtgtaaa ggcaaacgtt
    21061 taccttgcga ttttgcagga gctgaagtta gggtctggag tgaaatggaa tggcaacaat
    21121 aaaagatgta gcgaaacgag caaacgtttc cactacaact gtgtcacacg tgatcaacaa
    21181 aacacgtttc gtcgctgaag aaacgcgcaa cgccgtgtgg gcagcgatta aagaattaca
    21241 ctactcccct agcgcggtgg cgcgtagcct gaaggttaac cacaccaagt ctatcggttt
    21301 gctggcgacc agcagcgaag cggcctattt tgccgagatc attgaagcag ttgaaaaaaa
    21361 ttgcttccag aaaggttaca ccctgattct gggtaatgcg tggaacaatc ttgagaaaca
    21421 gcgggcttat ctgtcgatga tggcgcaaaa acgcgtcgat ggtctgctgg tgatgtgttc
    21481 tgagtaccca gagccgttgc tggcgatgct ggaagagtat cgccatatcc caatggtggt
    21541 gatggactgg ggtgaagcaa aagctgactt caccgatgcg gtcattgata acgcgttcga
    21601 aggcggctac atggccgggc gttatctgat tgaacgcggt caccgcgaaa tcggcgtcat
    21661 ccccggcccg ctggaacgta acaccggcgc aggccgcctt gccggtttta tgaaggcgat
    21721 ggaagaagcg atgatcaagg tgccggaaag ctggattgtg cagggtgact ttgaacctga
    21781 atccggttat cgcgccatgc agcaaatcct gtcgcagccg catcgcccta ctgccgtctt
    21841 ctgtggtggc gatatcatgg caatgggcgc actttgtgct gctgatgaaa tgggcctgcg
    21901 cgtcccgcag gatgtttcgc tgatcggtta tgataacgtg cgcaacgcgc gctattttac
    21961 gccggcgctg accacgatcc atcagccaaa agattcgctg ggtgaaacag cgttcaacat
    22021 gctgttggat cgtatcgtca acaaacgtga agaaccgcag tccattgaag tgcatccgcg
    22081 tttgattgaa cgccgctccg tggctgacgg cccgttccgc gactatcgtc gttaatcacc
    22141 cgttgcggga gtctcttccg gctcccgcag ccactcctta ttcagcgtct cactatcgct
    22201 gagatactca agcagccagg ttaacgcagg cgacatatca ttttgctgcc atgtcagaca
    22261 acatgccgaa tccgggaagg ggttttccag ttcaagtgct acccacttcc cctcattaag
    22321 ccacggtttg gcgaaatgtg ttggcaccat ccctatgcat aatcctgccg agatacaggt
    22381 tgccgatgat tcccagtcag gtacgacaag tcttttttgg ttatccagca accaggtaat
    22441 acgtttaggt agcgttcgcg aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt
    22501 atcatcgctg aacgggccat ccatcaacgc cagcgggtgg tgactggcaa caacgcaact
    22561 ccagcttagc atccccatat cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt
    22621 tgcgccaatt gccagttcca cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac
    22681 ttcctgaaag acaagaagtt cgacatcatc aaaatggcga taaaaatcaa cgatcatctg
    22741 ccgtgtacgt tctggcctga caatattatc cactgcgata gctaactgac cgcgccagcc
    22801 gttcgctatc tgctgacatt gctggcgggt gatctgcatt tttttgacaa cagagcgccc
    22861 ttctttgaga aaccacgctc cagcagcggt cagctccaca tcacggtgcc gtcgttcaaa
    22921 gagcggtacc gccagccact cttccagctg acgcacggta tagctgaccg cagaaggaac
    22981 acgatgcagc tcctgtgccg cagcgctaaa actaccatta cgcgctaccg catcaacaac
    23041 ttcgagtgaa tattctgacc acatagtctg cctgcaaaat ttttgaaacc agtcatcaaa
    23101 tattaccgtt tcacaacact aatttcactc cctacacttt gcggcggtgt ttaattgaga
    23161 gatttagaga atatacatgc aacctgggaa aagattttta gtctggctgg cgggtttgag
    23221 cgtactcggt tttctggcaa ccgatatgta tctgcctgct ttcgccgcca tacaggccga
    23281 cctgcaaacg cctgcgtctg ctgtcagtgc cagccttagt ctgttccttg ccggttttgc
    23341 cgcagcccag cttctgtggg ggccactctc cgaccgttat ggtcgtaaac cggtgttatt
    23401 aatcggcctg acaatttttg cgttaggtag tctggggatg ctgtgggtag aaaacgccgc
    23461 cacgctgctg gtattgcgtt ttgtacaggc tgtgggggtc tgcgccgcgg cggttatctg
    23521 gcaagcatta gtgacagatt attatccttc acagaaagtc aaccgtattt ttgcgaccat
    23581 catgccgctg gtgggtctgt ctccggcact ggctcccctg ttaggaagct ggctgctggt
    23641 ccatttttcc tggcaggcga ttttcgccac cctgtttgcc attaccgtgg tgctgattct
    23701 gcctattttc tggctcaaac ccacgacgaa ggcccgtaac aatagtcagg atggtctgac
    23761 ctttaccgac ctgctacgtt ctaaaaccta tcgcggcaac gtgctgatat acgcagcctg
    23821 ttcagccagt ttttttgcat ggctgaccgg ttcaccgttc atccttagtg aaatgggcta
    23881 cagcccggca gttattggtt taagttatgt cccgcaaact atcgcgtttc tgattggtgg
    23941 ttatggctgt cgcgccgcac tgcagaaatg gcaaggcaag cagttattac cgtggttgct
    24001 ggtgctgttt gctgtcagcg tcattgcgac ctgggctgcg ggcttcatta gccatgtgtc
    24061 gctggtcgaa atcctgatcc cattctgtgt gatggcgatt gccaatggcg cgatctaccc
    24121 tattgttgtc gcccaggcgc tgcgtccctt cccacatgca accggtcgcg ccgcagcgtt
    24181 gcagaacact ctgcaactgg gtctgtgctt cctcgcaagt ctggtagttt cctggcttat
    24241 tagtatcagc acgccattgc tcaccaccac cagcgtgatg ttatcaacag tagtgctggt
    24301 cgcgctgggt tacatgatgc aacgttgtga agaagttgac tgcccgaatc atggcaatgc
    24361 cgaagtcgct catagcgaat cacactgatc tatatcgata tacttatact taggctgcta
    24421 acaaaatttt gttgtatgat tgaaattagc ggcctatact aatttcgagt tgttaaagct
    24481 acgataaata ttatgttttt acggggacag gatcgttccc gactcactat ggatggtcat
    24541 ttcggcaagg gttcctcctt tccctctgtt ctacgtcgga ttatagactc gcggtttttt
    24601 ctgcgagatt tctcacaaag cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg
    24661 accagtgatg gagaaactat gagttcatcg tgtatagaag aagtcagtgt accggatgac
    24721 aactggtacc gtatcgccaa cgaattactt agccgtgccg gtatagccat taacggttct
    24781 gccccggcgg atattcgtgt gaaaaacccc gattttttta aacgcgttct gcaggaaggc
    24841 tctttggggt taggcgaaag ttatatggat ggctggtggg aatgtgaccg actggatatg
    24901 ttttttagca aagtcttacg cgcaggtctc gagaaccaac tcccccatca tttcaaagac
    24961 acgctgcgta ttgccggcgc tcgtctcttc aatctgcaga gtaaaaaacg tgcctggata
    25021 gtcggcaagg agcattacga tttgggtaat gacttgttca gccgcatgct tgatcccttc
    25081 atgcaatatt cctgcgctta ctggaaagat gccgataatc tggaatctgc ccagcaggcg
    25141 aagctcaaaa tgatttgtga aaaattgcag ttaaaaccag ggatgcgcgt actggatatt
    25201 ggctgcggct ggggcggact agcgcactac atggcatcta actatgacgt aagcgtggtc
    25261 ggcgtcacca tttctgccga acagcaaaaa atggctcagg aacgctgtaa aggcctggat
    25321 gtcaccattt tgctgcaaga ttatcgtgac ctgaacgacc agtttgatcg tattgtttct
    25381 gtggggatgt tcgagcacgt cggaccgaaa aattacgata cctattttgc ggtggtggat
    25441 cgtaatttga aaccggaagg tatattcctg ctccatacta tcggttcgaa aaaaaccgat
    25501 ctgaatgttg atccctggat taataaatat atttttccga acggttgcct gccctctgta
    25561 cgccagattg ctcaatccag cgaaccccac tttgtgatgg aagactggca taacttcggt
    25621 gctgattacg atactacgtt gatggcgtgg tatgaacgat tcctcgccgc atggccagaa
    25681 attgcggata actacagtga acgctttaaa cggatgttta cctattatct gaatgcctgc
    25741 gcaggtgctt tccgcgcccg tgatattcag ctctggcagg tggtgttctc acgcggtgtt
    25801 gaaaacggcc ttcgagtcgc tcgctaaagg ctattctatc gccccctctt cgggggcgag
    25861 ttcagatcag gcttctgtac ctggttgatt catggcattt tctcgtgccg ccagcacacg
    25921 ttctaccgta tctaccactg cctgagtttg tggatcgatt tcaatgttga cgcgtgcgcc
    25981 aagttttttc ttcccaagag tcgtgcgttc cagtgtttcc ggaattaaat ggacgcaaaa
    26041 acgcgttggc gtgacttcgc cgacggtcag gctaataccg tcgatgccaa taaatccttt
    26101 gtacaggata tatttcatca actgactatc ctggacttta aaccagatct ggcgattatt
    26161 ttccgaggtt aatattttcg ccacttcagc ggtggtcata atatgacctg acattaagtg
    26221 tccgccaatt tcatcactga atttcgccgc acgctcaacg tttacccaat cccccacttt
    26281 taaatcgcca agattggtaa tgcgtaacgt ttctttcatc aggtcaaaac tgacatggtt
    26341 gccgttaatt tccgtcacgg tcaggcagca accgttatgc gccacggaag caccggtttc
    26401 caggccgtcc agcatgtggt cgggtaactc caccacatgc gtacgaaaat ttggtttctc
    26461 gtcaatcgac accagttttg cggtgccctg tacaatcccc gtaaacatac ttacaactcc
    26521 tgaaatcagt taagacattc tgttcagcac aatagcaggt ggaaaacgcc cttaccagtg
    26581 aaggggtaag aatggctatt ttttcactgg agaattaata aatcctcgct acaatagact
    26641 gaatttcccc tgcttcttct ttttgctgcc cattcaggcg gctttttagt ctctcatata
    26701 actacaaata aaaggtgttc acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc
    26761 attagcaatc ccggtgattc tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac
    26821 cgtgatggcg ggcggctata gtgccaccga catggcggcg gtcgctatcg gtacttctat
    26881 ctggcttccg gcgatcctct ttggtcacgg actgctgctg gcattaacgc cggttatcgc
    26941 gcaattaaat ggttccggtc gacgtgagcg cattgcgcat caggtgcgac aaggtttctg
    27001 gctggcaggt tttgtttccg ttctcattat gctggtgctg tggaatgcag gttacattat
    27061 ccgctacatg gaaaacatcg atccggctct ggcggacaaa gccgtgggtt atctgcgtgc
    27121 gttgttgtgg ggcgcgccgg gatatctgtt cttccaggtt gcccgtaacc aatgtgaagg
    27181 tctggcaaaa accaagccgg gtatggtaat gggctttatc ggcctgctgg tgaacatccc
    27241 ggtgaactat atctttattt atggtcattt cggtatgcct gagctcggtg gcgttggttg
    27301 tggcgtggct actgcggcgg tgtattgggt catgttcctt gccatggttt cttacattaa
    27361 acgcgcccgc tccatgcgcg atattcgtaa cgaaaaaggc accgctaagc ccgagcctgc
    27421 ggttatgaaa cgactgattc aactcggttt gccgattgcg ctggcactgt tctttgaagt
    27481 gacactgttt gccgtcgtgg ctctgttagt gtctccgctc ggtattgttg atgtcgcagg
    27541 acaccagatt gccctgaact ttagttcact gatgtttgtg cttccaatgt cgctggcggc
    27601 agcggtaact atccgcgtag gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac
    27661 cgctgcgcgg accgggctta tggtgggtgt ctgtatggca accctgacgg ccattttcac
    27721 ggtttcactg cgggagcaaa tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct
    27781 ggctgcgcat ttgatgttgc tggcggcggt atatcagatt tctgactcaa tccaggtgat
    27841 tggcagtggg attttgcgtg gttataaaga tacgcgttcc attttctata tcacctttac
    27901 agcttactgg gtgctgggct tgccaagcgg ctatattctg gcactgaccg atctggtcgt
    27961 tgaacctatg gggccagcag gcttctggat aggctttatt atcggcctga catcggcagc
    28021 cattatgatg atgctgcgta tgcggttcct gcaacgtatg ccgtcagcca tcattctgca
    28081 acgagcgtcc cgctaataaa gacaaggcgc aaccttcacg ggttgcgcct gtgtttttac
    28141 gcaggctgga gcgttgcgcc aatcccgtct tcgtctggct gtaatttcag agcgttacac
    28201 agagtttgcc gactggcatc tgttatctca acgcgtatat ccccgttaac ttcccctttc
    28261 acattgatga agtcaggtga aatttctttc gcactggttt gcaggcaaat cgctccctct
    28321 ccgcgcaggc gtttcacttt cagttgcaca tgcgttgccg caccagtgat cagccacagt
    28381 tttccgccat tcagattgat gttaccaaca atgaaagtga cgctgtcacc ttttaccgta
    28441 ctgccgtcga gaatcaacac accgccctgc tgtacagtaa tcgttgattg ttgcagctct
    28501 gcgccataac caataatcat tgaagcgccg tccagtaatg caacactccc ggtcagttca
    28561 accggtgcgc gatcttcctc tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg
    28621 atcactgttc caccttgtat taccgcattt tccagcgtac cggcatgcac ttcagcccgc
    28681 ccccccacat tcaccgacag gttttgcgtc ttaccggaac tgtagagctg aaggccatcc
    28741 gtccccagac gataaatgga tccattctca acaatagtgt cagagtcttc accaccgaga
    28801 ttattcatgg tgccaccagc aagcaacgta tcactggctt tcccggccag cataaaaacg
    28861 ccgccctgat taatctcaca attgctggct tctccacttg gatacaccaa caattcacca
    28921 ccttcatcta ccacgaccgc tgaggttgtc ccattaacca acaggccgcc ctcttgatcg
    28981 agaatgattt tttccgcgcg atgtccttcc agtacacgca ggttaccgcc attttccagc
    29041 aacaaaccgc aggcataacc ctgatcaacg ctgaattcac cttcgggatg gcggccatta
    29101 accgtagcga gcgtagagag actaattgcg cctccctcgg actgaacaat atctgtggca
    29161 atggcaccgt cgctgaccga tagcgtgcca ttgttaccgt actgtgtgca tttggcgatc
    29221 ccaccggttg ccactttcaa ccagccagcc cccgtgacca cggtattttc ggctcttccg
    29281 ccagcttcaa caatcaactc gccttcagta agttgcgtcg cgccaaggac ctcgccggtc
    29341 ttaacaattt gcacattccc gtcgctcatc aagtttttcg catcagatcc catgatttat
    29401 tcctttgctg catctgtgtg cctttattgc tacctaagtg taaaggctac ggaggattta
    29461 tccacgacag atttgagatg gtggcaaaca actctgttta aactctgata cacgaattat
    29521 tgggttgtat cagatgtaaa tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa
    29581 tatagccaat ttgcttaaaa gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt
    29641 gcctcttgcc acctttccac tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt
    29701 gcgttcatag ctcagttggt tagagcacca ccttgacatg gtgggggtcg ttggttcgag
    29761 tccaattgaa cgcaccatcc tgcgtccgta gctcagttgg ttagagcacc accttgacat
    29821 ggtgggggtc ggtggttcga gtccactcgg acgcaccaga ttttcttaat ctggtctttt
    29881 cctttttccc tctgtttctt ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt
    29941 atgcgttatc agaaggagaa tcgctatggc aactttgtta caacttcatt ttgcttttaa
    30001 tggcccattt ggcgacgcaa tggctgagca gcttaaacca cttgctgagt cgattaatca
    30061 ggaacctggt tttctgtgga aggtatggac agaaagtgaa aagaaccacg aagccggtgg
    30121 gatctacctt ttcactgatg aaaaaagcgc ccttgcctat ctggaaaaac atactgcccg
    30181 actgaaaaat ctcggcgttg aggaagttgt cgccaaagtt ttcgatgtca atgagccact
    30241 tagtcaaatc aatcaggcaa aactcgcctg acagaattta atcaagggcg gttagcgccc
    30301 ttttcatccc tgtctgaaat ttctcaaatt ctaaaaatct caaccaaact tatctgataa
    30361 cactaaattc gaaagaatgc gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt
    30421 gccgggccta cggggatcta caccttattc tcgcttctac agcaacaaac tccactttct
    30481 atttctatct tcgagcaggc tgacgaggcc ggtgtcggga tgccatacag tgatgaggaa
    30541 aactcaaaaa tgatgctggc aaatattgcc agtattgaaa taccgccgat taattgtacg
    30601 tatctcgaat ggctacaaaa gcaagaagcc agccatctcc agcgttatgg cgttaaaaaa
    30661 gaaaccttgc acgatcgtca gtttttaccg cgaattctgc tgggcgaata tttccgcgat
    30721 caatttttac gattagtaga ccaggcacga cagcaaaaat ttgcagtggc tgtttatgaa
    30781 tcatgccagg ttaccgatct gcaaattaca aatgctggcg tcatgctcgc tacaaatcag
    30841 gatttaccca gcgagacgtt tgatttagcg gtgattgcca cgggtcacgt ctggcctgat
    30901 gaagaagaag caaccagaac gtattttccc agcccgtggt caggcctgat ggaagcaaag
    30961 gtcgatgcgt gtaacgtggg tattatggga acatccttga gcggactgga tgcggcaatg
    31021 gcagtggcta ttcagcatgg ttcgttcatt gaagatgata aacaacacgt cgtttttcac
    31081 cgcgataacg caagtgaaaa gctaaatatt acgttgatgt cgcgcacggg tattttaccc
    31141 gaagccgatt tctattgccc tattccctac gagcccttac acatcgtcac cgatcaggca
    31201 ttaaatgctg agattcaaaa aggtgaagag ggccttttgg atcgggtatt tagattgata
    31261 gtagaggaaa tcaagtttgc tgatccagac tggagccaac gcatagcctt agagagcctg
    31321 aatgtcgatt cctttgctca agcctggttt gccgagcgca aacaacgcga cccatttgac
    31381 tgggcagaaa aaaatctcca ggaagtcgaa cgcaataaac gagaaaaaca tactgttccc
    31441 tggcgttatg tcattctgcg cctgcatgaa gccgtacagg aaattgttcc tcatctgaat
    31501 gaacacgacc ataaacggtt cagtaaaggc cttgccaggg ttttcatcga taattatgcg
    31561 gcaatccctt cagagtctat tcgtcgacta ctggccttac gtgaagcggg gatctgaaat
    31621 ccctgatgtt ggcgaagatt atacgttaca gcaacccgaa gatattcgtg gacgcgtagc
    31681 gttcggcgcg ttgccctggt tgatgcacga ccagcctttc gttcagggac ttacggcatg
    31741 tgcagaaatt ggtgaggcga tggctcgggc ggtcgtaaag cctgcatccc gtgctcgtcg
    31801 gcgtctttcg tttgattaaa ggtaaagcta tcagcactca acaaactgat gctgttcatt
    31861 aagacgatag cggactccgg ttcgaatatt gttttcacct tcaatggcga cggcgaagcg
    31921 aactctttcg ccgtcatgat atgccagtgc ggcgcttccc cctagcccga gaataattga
    31981 gtcgaccacg cccgtgctca ctaccgttga attttcaccg ctggcaatga tgtgtacatt
    32041 gtcgccgctg ttggcaattc tggcgtttgc gccaaagctg gcgatttgca ccaggtcacc
    32101 attactggcg acatgacacc gttcacccaa tgtacaaacg cgtactcgca ttccggtatt
    32161 cgcaatccga ctactatctc cagcgctgct tatccgcgaa gaattaccgg ttacagcaac
    32221 ccgtgcgcgt tcaccggagc tgccaatgtg actgttaaag ccgacacttc caatccgcac
    32281 gctataaccc atactggcaa tttgcgcggc atacccagcg gttgcgattc gggcgttatc
    32341 ctcacaacag ccgatgcgag catgatcgtc gctgcgggca aactgactgc aaaatggtat
    32401 ctctccgggg tctgtggcca gtttttgcat agaggaaact tcctgatgcg cgaagttttc
    32461 atccgccagc cattttttcc acgcatactc aaccagactc tccgcccagt ccgtatatcc
    32521 ttcctcaatc agtgcatgac gaatatcggc ataactaccg ccgcgtggga aatggcgcag
    32581 gaaccagcga tacatcaccg ctccgatacg ccattctcgc aggtctgctc gtgtaatgat
    32641 cattcgttag tgccggtgat agccatcgac catgctttta aaggtttcgt gtggcgtacg
    32701 ccccgtggtg caaagataaa gatgaccgaa gataaaaaag aggcttataa atgccagagc
    32761 aaaatgcgcc tgcaataacc agtatcttac gccaggaaac acatctccca cggcttgcgg
    32821 atagagacac aacagccccg tcaatagtaa caacggcagc aatccataca tgacaccaac
    32881 ataggcgacc tgctgtaagg gattaaattt agactgtgtt gttgccggga aaggatgttc
    32941 ttccccctgc ataatgccaa acaaataaaa tcgcgtttgt tttgccgctc gttccagcca
    33001 cccctgacga cgaatgcgat agtggtgacc attatcccca acggcattga tcagcacaaa
    33061 gccgagccag catgccagta acaaaaatcc gcaaacttca tgcaccgcaa ccagactttt
    33121 aaccgcagtt gcgcccacca tcgcaaagtg atttatcagc ccacttgcca gcaacaatac
    33181 aaagagtaac gcattcgacc agtgccataa tctgaccgct ttgctgtaaa gataaacctt
    33241 ctcaccatga tcggtggctg atttcttcac gccacgagca cgaagcaagg cgtgcagccc
    33301 cagcaccaac cacatcccaa ccagcaatac tccggcaatg atcaaccaca ccggccaaaa
    33361 ttcgggagtg aactgtggca catagttcgc taactggctc tgaaactgtt cagcatgttg
    33421 cgacgggttc atacattttc ctttttaatc aaatgttgac cgaaccggcg atacagatgc
    33481 ggttttccgg caccaggtag ctgatattga tagtatttat tatcctgcaa ccacgcctga
    33541 atttctgggc tatcttcgcg accaaagatc aatgcgtgtt ccggacaggc gctgacgcaa
    33601 atgggcggaa agccttttgc cagtcgggac tcagcgcaga aatcacattt atctgccact
    33661 ttagtgacag ggttaaggta gcgaaccagg tacggacaag cgccaatgca ataactgcag
    33721 ccgatgcact gcgatttttc tacccgcacg atcccctgct catcacgcca cgatgccccc
    33781 gtcgggcata cgtcgatgca cggagcatct tcgcagtgct gacatgactg gcgaaaaaag
    33841 tgatactgcg tttcgttgtc gttatcggtg actggaatgt gtgctatcga taaacggctt
    33901 ccctgagcgg ggacgtggtt agttttacgg catgcgcggg cgcaaatatt acagccattg
    33961 caccgtgact cgtcatgaat catggcatac cgaacttcct tttcttgcct cgtgttcgct
    34021 aacagagaag acgcagaacc agtaaaaaag attactgttc ccatccccag aacaaatttg
    34081 cgtcgagtga aggacatctg attatcccct aatgcagtgg ctgcaactgt ccaccctatc
    34141 catgacatct ccctgaaatt tcaggggttc tgaaactgct ctacctcgaa ccattcgcat
    34201 aagccataag ccttgccata ggtcataaat cgctcgctaa acaagtacaa caccgccgtc
    34261 gatgactcca cctgccgtat atcggcatat tcaggtgcaa catccatcgc ggcgatcgcg
    34321 gcttcaatct gcgcttcctg gaagtaatat ggcgcttgca tcagcatggc gactttgtaa
    34381 ggacgaggat acgtctggca ctcaaaacga acggcgtgtg caatggcgcg acaaatgtcc
    34441 tgctccacaa cttgcaaaga catcgccgcg tagttttcac tcatggcctg cgttgagtaa
    34501 tagtaatcat cctgacttcc cgtcatacaa gcgatatccg cgtagtctcc gttctgctta
    34561 atgctttcca gcaaaactga cagttcctct tctgcaaccg aatagggtgg cgagagaaac
    34621 acggtgcgcg ccaccagctg cccagccgca gaatgctggc gaatgaactc agccaggatt
    34681 tcagcaggtt ctggttcggt taccgtatca acggggtgac gcatcccctg caaccattgc
    34741 cgcttcgcct cgtcaacttc gctgactttc gctaacggca attcatcctg atggttcatc
    34801 ttacccattc cttacgcagg cagtagattg tgtgctgcca gatcggcggc aatatcttcc
    34861 agccccagac gctgcaatgt ttcgcgggtt ggacaaccaa gctgtgggtc ccagcccatt
    34921 tctttgtaga acatcgtgag cgaagcatgc atatcgtcac gatccatttt gtcagtacct
    34981 tcagtaaaga ccgggatctg cggatccttg tcgaaaaccc aggaacagat aagatcgtgt
    35041 tcattacgca tatctttggt ttgcatcggt tttaccgtgt aggcacgatg caacgtaaag
    35101 atacgctctg cagctaaatc taatttttcc tgagtcatct cttcgccggt gatcgctttg
    35161 aagaatttgg cttcaagcgc cagatcgccc cggtaattac ggcttttcag cggcgaaacg
    35221 gtcattggcc agacccagtt gcacagagtg acggcgttat gcaaacagac ccgcaacagc
    35281 gaccactttg catatttaat ttttgcgtca ttgattggcg tgtagttttt ggtttcatcg
    35341 taagcatctt cagaaccaaa aagttctttc gccacttcac gttgcagttt caatggcaag
    35401 ccggaaccaa taaagttgat atgggtatgc gtcatgcagt cacggttgaa catacactta
    35461 acaatggaac cgacctgcgc tgacgcttca ttggcatggt gaaccggata gccaaacggc
    35521 gaccagagtt tatttttcgc gtagccccag tactctttac ccaaattcca gcgttctgcg
    35581 atggcatatg aaccatcagc caggtgactc agctcaccca cacgatgcgc cagacggtag
    35641 taaaaatctt taatgaagtt aacgtcaccc gcttccagtt gatcccagcg aatttctgca
    35701 tactcttcag ctggcagaac acgcttgaac acacctttgc tgtaacaata agtaaagtcg
    35761 cgatgcaact gcccgtagtt acaccatagg ccgtagtcat cgaacaaatt cagaccaacc
    35821 aggttaccaa tcacacgacc atcgtcttta tcttcaaaat ctttcggccc gttcgggaag
    35881 atggtggtat ggacaaagtt tgctacacaa gtgttaccac ctgtgctggg cacgccaaac
    35941 tctttcaccc gaggaatatt catttgggtc atacaacgga tcgggcaaga gtggcagccg
    36001 ctcattttta ctgtgtattt ctctgccgcc ggtcctaagt caaaaacgga tttataggta
    36061 cgaaagccga ccgtattctg attgcctggc ggaatttcac ccgtttcaat cggaccacct
    36121 tcagccgcgc cccaaaacag ccctttacgt gctgtccagc gtgacttggg atctgaatac
    36181 tctgcccacg attgtggcgt acttggcacg acatggttgt tattcgcacc aataagttca
    36241 gtcatcatgt aatcattgag acgcttcatc tcctgacgat cggcaatgtt gacccctttc
    36301 gtcccttcaa ccgcaatcgc tttcaggttt ttcgaaccca ttattgcgcc agttcccgca
    36361 ccgccgctgt ggttacggct attcaacatg ccagagagag gaataaggtt ttccccagcc
    36421 tgaccaatag ccgccacaca ggtttccggg ctggtcaatc gacaaatttc ttccgtcgtt
    36481 gcgcgcgtcc cttttcccca taagaaatcg gctttttcca ggctaacttt gtcatcttta
    36541 atcttcagcc ataccggtga tttcgccttc ccttcgataa taatgacgtc gtatccagcg
    36601 aatttcattt gcgctgcaaa aaagccaccc atatgggcat cgcgactaaa tttcctttgg
    36661 taaaagtaga aagtgaggtg atatttacgc gagaactaca gggggcacca gatccaggta
    36721 atgggccggt agcaaagact aatttattcg cttcatcgaa aggtttcgtg cctagcggta
    36781 cttcgtcata cataattttg tagccgaagc ccatgccacc gacaaaactt ttaaacttac
    36841 tggaatcttc gagggtaata tttcctgtcg tgagattgac tcttaatata ttacctgtcc
    36901 aaccgttagc catgattttt tcctttgcaa gattcacaca gtaatatctt tccactcgat
    36961 aatttttaac gcccctgttg ggcaggcgtt tgcgca
//
LOCUS       DAZXUK010000010        36658 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZXUK010000010 DAZXUK010000000
VERSION     DAZXUK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36658
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.010"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            58..414
                     /locus_tag="ACH3L8_000437"
     CDS             58..414
                     /locus_tag="ACH3L8_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFT4083705.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            418..1545
                     /gene="dapE"
                     /locus_tag="ACH3L8_000438"
     CDS             418..1545
                     /gene="dapE"
                     /locus_tag="ACH3L8_000438"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFT4083706.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            1573..1773
                     /locus_tag="ACH3L8_000439"
     CDS             1573..1773
                     /locus_tag="ACH3L8_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFT4083707.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1883..2581)
                     /gene="ypfH"
                     /locus_tag="ACH3L8_000440"
     CDS             complement(1883..2581)
                     /gene="ypfH"
                     /locus_tag="ACH3L8_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFT4083708.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(2655..4670)
                     /gene="tmcA"
                     /locus_tag="ACH3L8_000441"
     CDS             complement(2655..4670)
                     /gene="tmcA"
                     /locus_tag="ACH3L8_000441"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFT4083709.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(4685..5548)
                     /locus_tag="ACH3L8_000442"
     CDS             complement(4685..5548)
                     /locus_tag="ACH3L8_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFT4083710.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(5716..6429)
                     /gene="purC"
                     /locus_tag="ACH3L8_000443"
     CDS             complement(5716..6429)
                     /gene="purC"
                     /locus_tag="ACH3L8_000443"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFT4083711.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(6642..7676)
                     /gene="bamC"
                     /locus_tag="ACH3L8_000444"
     CDS             complement(6642..7676)
                     /gene="bamC"
                     /locus_tag="ACH3L8_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFT4083712.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(7693..8571)
                     /gene="dapA"
                     /locus_tag="ACH3L8_000445"
     CDS             complement(7693..8571)
                     /gene="dapA"
                     /locus_tag="ACH3L8_000445"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFT4083713.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            8717..9289
                     /locus_tag="ACH3L8_000446"
     CDS             8717..9289
                     /locus_tag="ACH3L8_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFT4083714.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            9289..9759
                     /gene="bcp"
                     /locus_tag="ACH3L8_000447"
     CDS             9289..9759
                     /gene="bcp"
                     /locus_tag="ACH3L8_000447"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFT4083715.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            10012..10629
                     /gene="hyfA"
                     /locus_tag="ACH3L8_000448"
     CDS             10012..10629
                     /gene="hyfA"
                     /locus_tag="ACH3L8_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFT4083716.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            10629..12646
                     /gene="hyfB"
                     /locus_tag="ACH3L8_000449"
                     /pseudo
     CDS             10629..12646
                     /gene="hyfB"
                     /locus_tag="ACH3L8_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            12657..13604
                     /gene="hyfC"
                     /locus_tag="ACH3L8_000450"
     CDS             12657..13604
                     /gene="hyfC"
                     /locus_tag="ACH3L8_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFT4083717.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQNYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            13621..15060
                     /gene="hyfD"
                     /locus_tag="ACH3L8_000451"
     CDS             13621..15060
                     /gene="hyfD"
                     /locus_tag="ACH3L8_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFT4083718.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVDFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            15072..15722
                     /gene="hyfE"
                     /locus_tag="ACH3L8_000452"
     CDS             15072..15722
                     /gene="hyfE"
                     /locus_tag="ACH3L8_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFT4083719.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            15727..17313
                     /gene="hyfF"
                     /locus_tag="ACH3L8_000453"
     CDS             15727..17313
                     /gene="hyfF"
                     /locus_tag="ACH3L8_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFT4083720.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            17303..19017
                     /gene="hyfG"
                     /locus_tag="ACH3L8_000454"
                     /pseudo
     CDS             17303..19017
                     /gene="hyfG"
                     /locus_tag="ACH3L8_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            19027..19572
                     /gene="hyfH"
                     /locus_tag="ACH3L8_000455"
     CDS             19027..19572
                     /gene="hyfH"
                     /locus_tag="ACH3L8_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFT4083721.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            19569..20327
                     /gene="hyfI"
                     /locus_tag="ACH3L8_000456"
     CDS             19569..20327
                     /gene="hyfI"
                     /locus_tag="ACH3L8_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFT4083722.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            20320..20733
                     /gene="hyfJ"
                     /locus_tag="ACH3L8_000457"
     CDS             20320..20733
                     /gene="hyfJ"
                     /locus_tag="ACH3L8_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFT4083723.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            20763..22775
                     /gene="hyfR"
                     /locus_tag="ACH3L8_000458"
                     /pseudo
     CDS             20763..22775
                     /gene="hyfR"
                     /locus_tag="ACH3L8_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            22797..23645
                     /gene="focB"
                     /locus_tag="ACH3L8_000459"
                     /pseudo
     CDS             22797..23645
                     /gene="focB"
                     /locus_tag="ACH3L8_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(23683..24744)
                     /locus_tag="ACH3L8_000460"
     CDS             complement(23683..24744)
                     /locus_tag="ACH3L8_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFT4083724.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            24957..26420
                     /gene="bepA"
                     /locus_tag="ACH3L8_000461"
     CDS             24957..26420
                     /gene="bepA"
                     /locus_tag="ACH3L8_000461"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFT4083725.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            26441..26800
                     /gene="arsC"
                     /locus_tag="ACH3L8_000462"
     CDS             26441..26800
                     /gene="arsC"
                     /locus_tag="ACH3L8_000462"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFT4083726.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(26933..27634)
                     /locus_tag="ACH3L8_000463"
     CDS             complement(26933..27634)
                     /locus_tag="ACH3L8_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFT4083727.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(27729..29018)
                     /gene="uraA"
                     /locus_tag="ACH3L8_000464"
     CDS             complement(27729..29018)
                     /gene="uraA"
                     /locus_tag="ACH3L8_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFT4083728.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(29104..29730)
                     /gene="upp"
                     /locus_tag="ACH3L8_000465"
     CDS             complement(29104..29730)
                     /gene="upp"
                     /locus_tag="ACH3L8_000465"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFT4083729.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            30054..31091
                     /gene="purM"
                     /locus_tag="ACH3L8_000466"
     CDS             30054..31091
                     /gene="purM"
                     /locus_tag="ACH3L8_000466"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFT4083730.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            31091..31729
                     /gene="purN"
                     /locus_tag="ACH3L8_000467"
     CDS             31091..31729
                     /gene="purN"
                     /locus_tag="ACH3L8_000467"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFT4083731.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            31901..33967
                     /gene="ppk1"
                     /locus_tag="ACH3L8_000468"
     CDS             31901..33967
                     /gene="ppk1"
                     /locus_tag="ACH3L8_000468"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFT4083732.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            33972..35513
                     /gene="ppx"
                     /locus_tag="ACH3L8_000469"
     CDS             33972..35513
                     /gene="ppx"
                     /locus_tag="ACH3L8_000469"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFT4083733.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(35552..>36658)
                     /gene="pdeF"
                     /locus_tag="ACH3L8_000470"
     CDS             complement(35552..>36658)
                     /gene="pdeF"
                     /locus_tag="ACH3L8_000470"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFT4083734.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFISNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         8489 a         9697 c         9531 g         8941 t
ORIGIN      
        1 aaaatgtttt cacccttaaa tgggtattta ttctcataaa tcgaaaaagg attcattatg
       61 gttacacttt acggcatcaa aaattgtgac accattaaaa aggctcgccg ttggctggaa
      121 gccaataaca tcgactatcg ttttcatgat taccgcgtcg atgggctgga cagcgaatta
      181 ttgaacggtt ttatcaacga attaggctgg gaagcgttac tcaacacccg tggtacaacc
      241 tggcgtaaac tggacgaaac tacccgcaat aaaatcaccg atgcggcctc tgcggcggca
      301 ttaatgactg aaatgcctgc aattatcaaa cgtccattgc tctgcgcgcc tggtaagcct
      361 atgctgctgg gtttcagtga ttccagttat cagcaatttt tccatgaggt gtagtctatg
      421 tcgtgcccgg ttattgagct gacacaacag cttattcgcc gcccttccct gagtcctgat
      481 gacgcaggat gtcaggcttt gttgattgaa cgtttgcagg cgatcggttt taccgttgaa
      541 cgcatggatt ttgccgatac gcagaatttt tgggcatggc gtgggcaagg tgaaacgtta
      601 gcctttgccg ggcataccga cgtggtgccg cctggcgacg ccgatcgttg gatcaatccg
      661 ccgtttgaac ccaccattcg tgacggcatg ttattcgggc gcggtgcggc agatatgaaa
      721 ggctcgctgg cggcgatggt ggtagcagca gaacgttttg tcgcacaaca tcccaaccat
      781 acggggcgac tggcatttct gatcacttct gatgaagaag ccagtgccca caacggtacg
      841 gtaaaagtcg tcgaggcgtt aatggcacgt aatgagcgtc tcgattactg cctggtcggc
      901 gaaccgtcga gtatcgaagt ggtaggtgat gtggtgaaaa atggtcgtcg cggatcgtta
      961 acctgcaacc tcaccattca tggcgttcag gggcatgttg cctacccaca tctggctgac
     1021 aatccggtac atcgcgcagc acctttcctt aatgaattag tggctattga gtgggatcag
     1081 ggcaatgaat tcttcccggc gaccagtatg cagattgcca atattcaggc gggaacgggc
     1141 agtaacaatg ttattccggg tgaactgttt gtgcagttta acttccgctt cagcaccgaa
     1201 ctgactgatg agatgatcaa agcgcaggta cttgccctgc ttgaaaaaca tcaactgcgc
     1261 tatacggtgg attggtggct ttccgggcag ccatttttga ccgcgcgcgg taaactggtg
     1321 gatgcggtcg ttaacgcggt tgagcactat aatgaaatta aaccgcagct actgaccaca
     1381 ggcggaacgt ccgacgggcg ctttattgct cgcatggggg cgcaggtggt ggaactcggg
     1441 ccggtcaatg ccactattca taaaattaat gaatgtgtga acgctgccga cctgcagcta
     1501 cttgcccgta tgtatcaacg tatcatggaa cagctcgtcg cgtgatgagt ggttctgcaa
     1561 gaggaaataa gcatggactg gctggctaaa tattggtgga ttctggtgat tgtctttttg
     1621 gtaggcgtcc tgctgaacgt gattaaagat ctcaagcgcg tcgaccataa gaaatttctc
     1681 gccaacaagc cggagcttcc cccgcatcgt gatttcaacg ataagtggga cgatgacgac
     1741 gactggccga aaaaggatca accgaagaag taagctgagt gctcaaccgt aggccggata
     1801 aggcgttaac gccgcatccg gcaatggtga acgatccctg atgcgacgct cacgcgtctt
     1861 atcaggccag gtaatctgtc ttttacatca tttcaatcac atcatcatcg ccaggcttac
     1921 cgccacttag cgcttggtcg aaataatgtt tcggaatggt ataacgcaag tgatccaggg
     1981 caaattgcat actgcgattg tcgatcgcat ggcctaaatc ctccacgata tccagcgtca
     2041 cgtcaccacc ggcactgatt aacgcttcct gcgccgcaac tgcatgcgcc agatcgataa
     2101 ccggatcttc accaccatga atcaagtgaa ttgtcgtggc tgtagacgcg gtctccggca
     2161 ggctcgcata gcgtccgtta aaggcaatga cgcgcgaagc aaggcccggt tcggctttaa
     2221 tgctctccag cgccataatc gcgccttgtg agaaaccaat tagcgccgtg gcgttagccc
     2281 ctactccgct ctgtttctgc cagtagcgta ccgtttcaat aaacgtcggc atgatggcat
     2341 ccacacgcgc ctggcgatta tcttccgtaa taccctgcac tgaaaaccac tgacgccccg
     2401 ccggattacc gctcggctcc gcgccaccga cactcaccac cagcgcatca ggaaacagcg
     2461 gtgcaaacca gctgcctatt tcccccattg ccaccgggtt atccccgaca ccgtggaaaa
     2521 gcagtaacaa ctgttgtgca ggtttatccg ggctttgaac aacaaaatgg tcatgtttca
     2581 tggcgaactc cttaactgat gtcattaatt ttacgccgct gactcacaat gaccatggga
     2641 gaaaattgaa ggagtcagtg aaaaaattgc cattgcgtta tgcgatcgcg cagacgctca
     2701 gtgcgaacct catttagtgc gaacagcgcc tgcgcggcct cttcccgctg gcgaaccagt
     2761 aacatcttgc ggcctgaaag tttaagtgtg gtacataact gcgcgtcact ggcgtttttc
     2821 tgtaaacgcc cacgcagcgc cggtaatgcc agttcactgg tttgtagcag acgcaataag
     2881 caacctaacg atgttaatag cggacgatga gcgaaagcaa aaccggccag ttcaagccag
     2941 tcgtcgtcag aaaggatggc atcgtttagt ggatcaacag gaagcatttc gccattccac
     3001 tgcgcgagag cttgcgcatc gcgacgtaaa cggtaatgct cacgttcagc cagctgttta
     3061 cccgcatcac tcatcggtaa cagcgccatc gccgtatagc aaccgctgct ggcttcccga
     3121 tgattaccca tccgcaccag cacaaaaccg cagcgttgcc agaaacgcca taactcccca
     3181 gtgtaaccaa aactcaccga aagatagtcg aggtcatgcg tatattgcaa agctccagca
     3241 ataagttgtc gccctgtgcc ttcccgctga cgagccggat gaactgctat ccggctaacc
     3301 cgccttccgc gcaatgtcgc cgccagtgga ttgctgccgt gcgccgccag cgactgggcc
     3361 accagattac cccgcgggcg acgaaaacct gcccataccg cctgactgag ttcttgagat
     3421 aatccgccct catccaccag ccacaacgcc ccggcaatct cgttttcgcc agccgcctgt
     3481 aaaaaatgtt gccctggtgc atccatcatc cggcgtaaat ccagcggcga ggtccggtag
     3541 tgcgcaccag acaataactg ataaaccttt aacggcgttt ctggctcgct tcgccataac
     3601 gtttgttcaa atgcggaaat gacgatattg ccttgtggtg tatgggtgaa gttttcatcg
     3661 ttaaaaacca gtgcctcact aaccattttt tccagcgggc atccctgtgc ccagcggatc
     3721 ggctgttgca gttcaaaacg gtgtaaatgc ggaaagcgag cgcaaaattt cagcaaaaaa
     3781 ccacgtccgg taccttcgta gccctgcacc gtagtggtta acaacgttcg aggaaaacgc
     3841 gataccagtt gatgcaacaa cggcgcaggt atggctgcgg cttcgtcgac caccagccag
     3901 tcggcttgct catcgctggc taacaaggca tccggtgcaa taaagcgaaa cttctcgccc
     3961 gcaaattgtg ccagtacatc cgttgccgct tttgcgggcg cggtgacaat cgcactgccc
     4021 gcaatacgag aaatgagttg ccctgccaac gccgacttac cgcgcccacg cgcagccgtt
     4081 accgctgcca cgcccggcgg catggtcagt agctgctgta agagttgctg ttgttctggt
     4141 tgtggtgcgc cggtcgcggg gtgccagtca gtacggggag taaaatgcgc caacgagaac
     4201 ggctggtttt gccgccagag gatagcgtcg ttatccgccg taagtacgcg tttgagatgc
     4261 tggacaaaat gcggcgtcgc aatagggtca gggcaatcac tccagcgcag cgagtcggca
     4321 tcaggttggt tttcccactc ttcccataca gggagtaaca aaaccagcca gcttcccgct
     4381 ttcaacgttc cgctaagtgc tgcaaaggca gcggcatcaa agccgtggcg ggcgtcgaat
     4441 accgcatgcc ggaactcgcg cccaagtaaa gtttgtagcg ccgagggtga acagtggttt
     4501 tcagcatcta gctgcggcga aatccacagc cagtcgccag gtaaggcatc acgcaactta
     4561 agagcatgat caaaacacca accctcttcc ccgctcaaca ccagcaagcg gcagatccct
     4621 tcacgtttca tttgcgctgt taatgtgtaa agcgcagtca gttcagccat ccctgccccg
     4681 gaaattaaat gcttttacca aaagtattgc attgtgccgg atcgccgctg tcgaaaccac
     4741 gtttaaacca gctgtagcgt tgctgagaag tgccatgtgt gaaactgtct ggtactactc
     4801 gcccctgact ttgctgttgt aaacggtcat cgccgatggc ctgcgccgca ttcagcgcct
     4861 cttccagatc gccagtttcc agaacgcctt gctgctgcat actatgcccc cagacaccgg
     4921 caaaacagtc ggcctggagt tccatacgca cagataagcg gtttacttcc gcctgcgtcg
     4981 cgttttgttg cagttgacga actttcggct cgatgcctaa cagtttctgc acatgatgac
     5041 cgacttcatg ggcgataacg tacccctggg caaaatcgcc atccgcgcca agtttgtctt
     5101 tcatgtcatc atagaaggag agatcgatat aaactgtgcc atccgccggg caatagaacg
     5161 gccccataat ggactggccc gccccgcagc cggtgcgcgt cattccacgg tacatgacca
     5221 gcttcggttg ctgataggtc ttacccatct tttcgaactg ttgtccccag gtgtcttccg
     5281 tggttgcctg aatcaccgag gtgaattttg cggcttcgtc ttcattaggg ctaattgacc
     5341 gcgttgattg ttgttgagaa accggctgcc cggtcatcaa cccggttaaa tcaacgccat
     5401 agtagcctgc aaccagcacg actatcagta aaatcagccc gcctttaccg ctcggcagac
     5461 ggaaaccggg accgcccatt gacggaccgc cagagctgtt gcgcctgtct tcaacattgt
     5521 cactttcacg tcgcccttgc caacgcatag atacctcaac aatatattca ttatagaaat
     5581 gatcgtaggt ggttcagagg aagattacca caggaaagcg ggatgaaatg gcggaggaat
     5641 gagcgttcaa tgcatatatt ccgagagcgg ttgtcttgcc gtgccagctg cacggcaaga
     5701 tgatgaacag aaaaatcagt ccagctgtac acccaggcgg cgggcgacgg cttcataggc
     5761 ttcgatcagg ccaccgaggc tctggcggaa acggtctttg tccattttct ccagtgtttc
     5821 tttgtcccac aggcggctgc cgtccgggga gaactcatcg cccagtacca cttcaccttt
     5881 gtacagacca aactccagct tgaagtcgac cagaatcaga ccagcatcat cgaacagttt
     5941 tttcagcacg tcgttcgctt tgtaggtcag ctctttcata cgcgccaggt tctctttgct
     6001 cacccagcca aaggtttcgc agtaagattc gttgaccatc ggatcgtgca tggcatcgtt
     6061 tttcaggaac agatcgaaca gcggcgggtt cagctcaata ccttcttcga ttccaagacg
     6121 tttcactaga gagccagcag cacggttacg cacgacacac tcaaccggca ccatatccag
     6181 ctttttcacc agacattcgg tatcggagag cagacgctcc atttgagtcg ggatacccgc
     6241 ttcagccagt ttgctcataa tgaagtagtt gaacttgttg ttcaccatac ctttgcgatc
     6301 aaactgctca atgcgcgcgc catcccctgc tgacgtatca ttgcggaatt cgagcaccaa
     6361 caggtccggg ttttccgtgc tgtatacggt tttcgcttta ccacgataca actcagcttg
     6421 cttttgcatc tttatcactc ctgggtgtga attaacgttt taaaatcttt tgctgtctgg
     6481 tgtgccggat gttttgtcgg atgcggcgtg aacgccttat ccgaactacg tccgacaaac
     6541 aaatttcgtg cgaattaccg ctaaaatcgc gttttcctgc cgacgcacac gtttgcgtat
     6601 catatcagaa aaaagggccg gatgattcca gccctgtatt tttacttgct aaacgcagcc
     6661 tggaagacag ctaccagcgc gtcgttctga ctctgagtca gagtatgacc tttcggatcg
     6721 atgaactgta ggctgctgcg gttatctaaa tcgccaacct gcagtttata gtcaccggat
     6781 gccaggcctg gatcgctcgc gcccagttcc tgccagtcgc tgtcagacag cggcttataa
     6841 gttacggcca tgttgccctg cgaacgggtg ctgtcggtca ctttcatgcc cactttttcc
     6901 agcgccgctg gcagacgttg ccaaaccaca ttgaacggcc cgcgtacgac cagcattggt
     6961 aaaccggtgt catcagctgc actttgtacg tccatagtgg tggaggcacg attttgcgca
     7021 gcgttcgcgg cgtcagtggc agatttatcc agaccggcgg aaataacgtt catcatctcc
     7081 gtgctgtaac gctgcatgga agccgcgtct gcaaccggtt tgcccgcctg ttccaggttc
     7141 agcagtttaa ccgtaaccgc ctgctgataa ccctgcggct taacagagat ttgataacga
     7201 ccacgatact gctcgtcttc gtccagacgg ttccattgta cccaatcggt ggtcagtgtc
     7261 tgaccagcat catcacgttg ggtgatggtg tagtttttcg cctgcagcac gctaaccacc
     7321 tgcggccaca gagtattgcc acgaccattt tccaccagca atgaagcggt atcgcccgtg
     7381 aactgggtac gcgcgccaga aaccagtgcc aacggctggg ctggtggacg aatgtccagc
     7441 gccttaccga cagcaccact accgttggtc accgggattg cataatcacc ggaggtcacc
     7501 ggcaaaatca ttccagccgg ggcatgaagc tccgcaagcg gtgccgcttc caggtaggct
     7561 tcatcaccac tgacctgacg cttatagcgt gagtcagaac tacaggcagc gagtaataaa
     7621 acaagcgaaa cacccgcaac ctttgccagg cgcgactttt gaacagagta agccatcaaa
     7681 tctccctaaa ctttacagca aaccggcatg cttaagcgcc gctctgaccg tctcacgacc
     7741 actgtcggtg attggtgtca ttggcaggcg cagcgtatcg gtcgccacaa gacccagttc
     7801 cttacatgcc catttcaccg ggattggatt gggttcgaca aatagtttgt tgtgtaatgg
     7861 catcagacgc tgattaataa cgcgtgcctc ggcaaaatgc ccttctgctg ccagtttgca
     7921 catctgggcc atatcacgcg ctgcgacgtt agccgtaacg gaaataactc catgaccgcc
     7981 caattgcatg aagtccagcg cgctcgcatc atcgccgctc agcagaacaa aatcatctga
     8041 aaccagctct ttgatctggt ttacacgcgt taagttccct gttgcctctt tgattccgat
     8101 aatatttttt actttcgcca gacggcccac cgtttccggg agcagatcgc agccagtacg
     8161 ggacggcaca ttatacagaa tttgcggcag gtcagtatgc tcagcgatgg ctttgaaatg
     8221 ctgatacaaa ccttcttgcg acggacgatt gtagtaaggg gttaccgtca ggcagccgac
     8281 gataccactg tcattgaagc gctgcgtcag gctaatggct tccgcagtag cgttagcgcc
     8341 ggtcccggca attaccggaa tgcgcccatc agccagatcc agcgtcatca tcaccacatc
     8401 agcatgttcg tcatgattta aggtagcgga ctcgccagtg gtgccaacag aaacgatcgc
     8461 cgaagtaccg ctggcgacat gataatcaat cagttttttc aagctagccc gacagacatt
     8521 acctttttca tccatcggag taacaatcgc gacaatactt cccgtgaaca tgggccatcc
     8581 tctgtgcaaa caagtgtctc aatggtacgt ttggtatggc attaaaagca agcagacaga
     8641 accgttctga ttgttgtatg catgattttt ttatgctttc cttaagaaca actcacccct
     8701 taaaggaata accagtttga cactgtcatc gcaacattat ctggtgatca ctgcgttggg
     8761 tgccgatcgc cctggaattg tgaacaccat cacccgtcat gtcagtagtt gcggctgtaa
     8821 tattgaagac agtcgcctgg cgatgctggg agaagagttc acgtttatca tgctgctttc
     8881 cggttcatgg aatgccatca ctctgattga atcaacgtta ccgttgaaag gtgccgaact
     8941 ggatctttta atcgtgatga agcgcacgac ggcgcgtccg cgtccgccaa tgccagcatc
     9001 tgtctgggtt caggtcgatg ttgcagactc cccgcattta attgaacgct tcacagcact
     9061 tttcgacgcg catcatatga acattgcgga gctggtgtcg cgcacacaac ctgctgaaaa
     9121 tgaacgggct gcgcagttgc atattcagat aaccgcccac agccccgcat ctgcggacgc
     9181 agcaaatatt gagcaagcgt tcaaagccct atgtacagaa ctcaatgcac aaggcagtat
     9241 taacgtcgtc aattattccc aacatgatga acaggatgga gttaagtaat gaatccactg
     9301 aaagccggtg atatcgcacc gaaatttagc ttgccggatc aagacggaga acaagttaat
     9361 ttgaccgact tccagggaca gcgtgttctg gtttatttct acccgaaagc catgaccccc
     9421 ggctgtaccg tacaggcctg cggcttacgc gataacatgg atgagttgaa aaaagcgggc
     9481 gttgatgtgc tgggtatcag caccgataaa cccgaaaaac tctcccgttt tgcggaaaaa
     9541 gagctgctta actttacgct cctgtctgat gaggaccacc aggtgtgcga acaattcggc
     9601 gtctggggtg aaaagtcctt catgggcaaa acctacgatg gtattcatcg catcagcttc
     9661 ctgattgacg ctgatggcaa aatcgaacat gtctttgacg atttcaaaac cagcaatcac
     9721 cacgacgttg tgctgaactg gctgaaagaa cacgcctgat tactttgctc cattccgtgc
     9781 tggctgcgct tgcggccagc atacctcact tctcgtgatc aagatcacat tctcgctttc
     9841 ccctgcgaca cgggtgtcga atccattttt tgctgaacgt taatgaccat catttttgta
     9901 ccgttcagaa tccagttaat acataactta ttgaatagat tgagttaatc agaatggcat
     9961 cctttataca atatgaaatg caatgtttca tatcattttc aaggagccga catgaaccgc
    10021 tttgtggtgg ccgaaccact gtggtgtaca ggatgtaata cctgtctcgc tgcctgttcg
    10081 gacgtgcata aaacgcaagg tttacagcaa cacccgcgcc tggccctggc gaagacgtca
    10141 acaatcactg cccctgtcgt gtgtcatcac tgtgaggaag ccccttgcct gcaggtctgc
    10201 ccggtcaatg ccatctctca gagggatgat gcgatccaac tcaacgaaag cctctgtatt
    10261 ggctgcaagc tttgcgccgt ggtctgccca tttggcgcaa tcagcgcttc aggaagccgt
    10321 ccggtgaatg cccatgcgca atatgttttt caggctgaag gctcactcaa agacggcgaa
    10381 gaaaacgtgc cgccccaaca tgcgttgctg cgctgggaac cgggtgtcca gaccgtcgcg
    10441 gtgaaatgcg acctgtgtga tttcttgcca gaaggtccgg cctgcgttcg cgcttgcccg
    10501 aatcaggcgt tacggctgat caccgacgat agcctgcaac gtcagatgaa agaaaaacag
    10561 cgccttgccg caagctggtt tgccaatggc ggggagtctc ccctttccct cactcaggag
    10621 caacactaat ggatgccctg caattattaa cctggtcgct gatcctctat ctgtttgcca
    10681 gtctggcttc gctgttttta ctcggtctgg acagactggc tattaagctt tccggcatca
    10741 catcgctggt gggcggcgtg attggcatca tcagcggaat tacgcaatta catgctggtg
    10801 taactttagt cgcccatttt gccacccctt ttgactttgc cgatttaacc ctgcgaatgg
    10861 atagcctctc ggcatttatg gtgctagtta tctccttgct ggtggtggtt tgttcgctct
    10921 attcattgac ttatatgcgc gaatacgagg gcaaaggcgc ggcggcgatg ggcttcttta
    10981 tgaatctttt catcgcatcg atggttgccc tgctggtgat ggacaacgct ttttggttca
    11041 tcgtgctgtt tgaaatgatg tcgctgtctt cctggtttct ggtcattgcc aggcaggata
    11101 aaacgtcgat caacgctggc atgctctact tttttatcgc ccacgccgga tcggtgctga
    11161 ttatgatcgc cttcttgctg atggggcgcg aaagcggcag cctcgatttt gccagtttcg
    11221 cacgctttca ctttctccgg ggctggcgtc ggcggtgttt ctgctggcct ttttcggttt
    11281 tggcgcgaaa gccgggatga tgccgttgca cagctggttg ccgcgcgctc accctgccgc
    11341 accatcgcac gcttcggcgt tgatgtctgg cgtaatggtc aaaatcggta ttttcggcat
    11401 cctgaaagta gcgatggatc tgctggcgca aacgggtttg cctctgtggt ggggcattct
    11461 ggtgatggcg atcggcgcaa tctccgcgct cctgggcgtg ctatatgcgc tggcggaaca
    11521 ggatatcaaa cggctgctgg cctggagtac cgtcgaaaac gtcggcatta ttttgctggc
    11581 agtcggtgtg gcgatggacg gtctgtcact gcacgacccg ctgctcaccg tggttggact
    11641 gctcggcgca ctgtttcatc tgctcaacca tgcgctgttc aaagggctgc tatttctcgg
    11701 cgcggacgcg attatttcgc gtttgcatac ccacgacatg gaaaaaatgg gggcactggc
    11761 gaaacggatg ccgtggacag ccgcagcatg cctgattggt tgcctcgcga tatcagccat
    11821 tcctccgctg aatggtttta tcagcgaatg gtacacctgg cagtcgctgt tctcactaag
    11881 tcgtgtggaa gccgtagcgc tacaacttgc gggtcctatt gctatggtaa tgctggcagt
    11941 cactggtggg ctggcagtaa tgtgcttcgt caaaatgtac ggtattactt tctgtggtgc
    12001 gccgcgcagt acacacgctg aagaggcaca ggaagtgcca aatacgatga tcgtcgccat
    12061 gctactgctc gcggcactct gcgtattaat tgcgcttagt gccagttggc tggcaccgaa
    12121 gataatgcat attgcccatg cgtttaccaa tacccctccc gtcactgtcg ccagcggaat
    12181 agcacttgta cccggcacgt ttcatacacg ggtcactcct tcattactgt tgctgttact
    12241 actggcgatg cctttgctgc caggccttta ctggctgtgg tgtcgttcgc gccgcgcagc
    12301 gtttcgtcgc acaggagatg cctgggcatg cggctacggc tgggaaaacg cgatggctcc
    12361 gtcaggcaat ggcgtgatgc agccgctgcg tgtggtcttc tctgcgttat ttcgtctacg
    12421 acaacagctc gaccctacgc tgaggctaaa taaaggtctt gcgcacgtca ccgccagggc
    12481 tcagagcaca gaacccttct gggatgagcg ggtgatccgc cccatcgtga gcgccaccca
    12541 acggctggtc aaagaaatac agcatctgca aagcggcgac tttcgtctct attgcctgta
    12601 tgtggtcgcc gcactggttg tgctgctaat cgctattgcc gtctaaggaa atcaccatga
    12661 gacaaactct ttgcgacgga tatctggtca tttttgcgtt agcacaggcc gtgattctgc
    12721 tgatgctaac cccacttttt acgggtattt cccggcagat acgcgcgcgt atgcactccc
    12781 gccgcgggcc ggggatctgg cagaattatc gcgatatcca caaactgttt aaacgccagg
    12841 aagttgcgcc gacatcttca ggtctgatgt tccgcctgat gccgtgggta ttaatcagca
    12901 gcatgctggt gctggcgatg gccttaccac tatttattac cgtttcccct tttgcgggcg
    12961 gcggcgatct gatcaccctt atctatcttc ttgccctgtt tcgttttttc tttgctcttt
    13021 ccgggctgga taccggaagt ccgtttgcgg gagtcggtgc cagtcgcgag ttgacgctcg
    13081 gcattctggt cgaaccaatg cttattctct cactgctggt attggcgctg atagcaggtt
    13141 ccacgcatat cgagatgatc agcaatacgc tggctatggg ctggaactcg ccgctaacca
    13201 ccgtactggc gttactggcc tgtggttttg cctgcttcat tgagatggga aaaattccct
    13261 ttgatgttgc tgaagcagaa caggaattac aggaaggccc gctgaccgaa tattccggtg
    13321 ccgggctggc gctagcgaaa tgggggctgg ggctgaaaca ggtcgtgatg gcagcactgt
    13381 ttgtggccct gtttctgccc tttgggcgcg cgcaagaact ttctctcgcc tgcctgctga
    13441 cttcacttgt cgttacgctg ctcaaggttt tgctgatttt tgtactggcc tcaatcgcag
    13501 aaaacacgct ggcacgcggg cgttttttac tcattcacca tgtgacctgg cttggcttca
    13561 gccttgctgc gcttgcatgg gtcttttggt taaccggtct gtaaggagca ctgacggaat
    13621 atggaaaatc ttgctctgac gacgttaatg ctgcctttta tcggcgcact ggtcgtttcg
    13681 ttttcgccac aacgtcgggc cgccgaatgg ggggttttgt tcgccgcgct gaccacgctg
    13741 tgcatgttgt cgctgatctc cgcgttttat caggccgata aagttgccgt cacgttgacg
    13801 ttggtcaacg tgggggatgt ggcattattt ggcctggtca ttgatcgcgt gagtacgctg
    13861 attctgtttg tggtggtgtt tctcggtttg ctggtcacga tctactccac gggttatctg
    13921 acggataaaa atcgcgaaca cccgcataac ggcacgaatc gttattacgc atttttgctg
    13981 gtgtttatcg gcgcgatggc gggactggta ctctcctcga cgctgctcgg tcagttgttg
    14041 ttttttgaaa ttacgggcgg ctgctcctgg gcgttgatca gttattacca gagcgataaa
    14101 gcgcagcgtt cagcactaaa agcgttgctt atcacccata tcggttcgtt ggggttgtat
    14161 cttgccgccg ccacgctgtt tttgcagacc ggaacgtttg tgcttagcgc gatgagcgag
    14221 ttacatggcg acgcacgtta tctggtttat ggcggcatcc tgtttgccgc gtgggggaaa
    14281 tcggcccagc taccgatgca agcgtggcta ccggacgcaa tggaagcgcc aacaccgatc
    14341 agcgcctatc tccacgccgc atcgatggtg aaagtgggcg tttacatttt tgcccgcgcc
    14401 attatcgacg gcggcaatat cccgcatgtg attggcggcg ttggcatggt catgacactg
    14461 gtcaccattc tttatggctt tctgatgtat ttgccacagc aggatatgaa gcggttgctg
    14521 gcctggtcga ccatcactca acttggctgg atgttcttcg gcttgtcgct ctccatcttc
    14581 ggctcgcggc tggcgctgga gggtagcatc gcctacatcg tcaaccacgc gttcgctaaa
    14641 agcctgtttt tccttgtagc aggtgcgctg agttacagct gcggcacgcg cttgttgccg
    14701 cgtctgcgtg gcgtattgca caccctgccg ttgccaggcg tggatttctg cgtggcagcg
    14761 ctggcaatta ccggtgtgcc gccgttcaac ggcttcttca gtaaattccc gctgtttgcc
    14821 gccggttttg cgttgtcagt ggagtactgg atcctgctgc ccgccatgat tctgctgatg
    14881 attgaatcgg tcgccagttt cgcctggttt attcgctggt ttggtcgcgt cgtgccgggc
    14941 aaaccgagcg aggccgtcgc cgatgccgca ccgctgccag ggtcaatgcg cctggtgttg
    15001 attgtactga ttgtgatgtc gctgatttcc agcgtaatcg ccgcgacctg gttgcagtaa
    15061 ggagatgatg aatgaccggt tctatgatcg taaataatct ggcgggactg atgatgctga
    15121 catcgctgtt tgtgattagc gtcaaaagct atcgcctgtc atgcggattt tacgcctgcc
    15181 agtcgctggt gctggtgtct attttcgcca ctctctcgtg cctgttcgcc gcagagcaac
    15241 tgctgatctg gtccgccagc gcctttatca ccaaagtgct gctggtaccg ttaatcatga
    15301 cttacgctgc acgaaatatt ccccagaaca tcccggaaaa agcgttattc ggtccggcaa
    15361 tgatggcact gctcgcggcg ttaattgtcc tgctttgcgc atttgtcgtt cagcccgtga
    15421 agctaccgat ggctaccggg ctgaaaccgg cgctggcggt agcgttaggt cattttctgc
    15481 ttggcctgct gtgcattgtc agccaacgca atatcctgcg gcaaattttt ggttactgcc
    15541 tgatggaaaa cggctcccat ctggtgctgg cgcttcttgc ctggcgagca ccggaactgg
    15601 tggaaatagg tatcgctacc gacgccatct tcgccgtcat tgtgatggtg ttactggcaa
    15661 gaaaaatatg gcgtacccac ggcacgctgg acgtgaacaa cttgaccgcg ctgaagggat
    15721 aatgagatga gttattctgt gatgttcgct ttactcctgc tcacgccgct gcttttttcg
    15781 ctgctctgtt ttgcctgccg gaaacggggg ctctctgcga cttgtacggt gactgtatta
    15841 catagcttag ggatcacact gctgctgatt ctagcactct gggtggtcca aactgccgct
    15901 gatgcaggag aaatattcgc tgcgggactg tggcttcata ttgatggtct gggcggtttg
    15961 ttcctcgcca ttcttggtgt gattggcttt ctcaccggtg tttactcgat tggctacatg
    16021 cgtcatgaag tggcacacgg cgagctttca cccgttacac tgtgcgatta ctacggtttc
    16081 ttccatctgt ttttgttcac catgctgctg gttgttacca gcaataacct gattgtgatg
    16141 tgggcggcga tcgaagccac caccttaagc tcggcgtttc tggtaggcat ttacggtcag
    16201 cgttcatcgc tggaatcagc atggaagtac atcattattt gtactgttgg tgtcgctttt
    16261 ggtctgttcg gtaccgtgct ggtatacgcc aacgccgcca gcgttatgcc gcaggcagaa
    16321 atggcgatat tctggagcga ggttcttaag caatcgtcct tgcttgaccc aacattaatg
    16381 ctgttggcct ttgtgtttgt gctaattggc tttggtacca aaaccgggct atttcccatg
    16441 cacgcctggc tgccggatgc tcacagtgaa gcgccgagtc cggtcagcgc cctgctctcc
    16501 gccgtattgc tgaactgcgc gctgttggtg ctgattcgct attacatcat tatttgccag
    16561 gccatcggca gcgatttccc caaccggttg ttgctcatct tcggcatgtt gtcggttgcc
    16621 gtggcggcat ttttcattct ggtacagcgg gacattaagc gtctgctggc gtactccagc
    16681 gtggagaaca tggggctggt cgcggtggcg ctaggcattg gcgggccgct gggaattttt
    16741 gccgcgctgc tgcacacctt aaaccacagt ctggcaaaaa cgctgctgtt ctgcggttcc
    16801 ggcaatgtac tgctcaagta cggcacgcgc gatctcaacg tcgtctgtgg gatgctcaaa
    16861 atcatgccat ttaccgccgt gctgtttggt ggcggtgcgc tggcgctggc gctggcaggg
    16921 atgccgccct tcaacatttt tcttagcgaa tttatgaccg ttaccgcagg actggcacgt
    16981 aatcacctgc tgattatcgt cctgctgtta ttgctgttaa cgctggtgct ggcgggcctg
    17041 gtacggatgg ctgcgcgggt gttaatggcg aaaccgccgc aggccgttaa ccggggtgat
    17101 ctcggctggt tgaccacctc gccaatggtg attctgctgg tcatgatgct ggcgatggga
    17161 acgcatattc cacaacctgt catcaggatc ctggcgggcg cttccactat agtcctctca
    17221 gggacgcacg acctgcctgc acaacgtagc acctggcatg attttttgcc ttcaggcacc
    17281 gcatctgttt cggagaaaca cagtgaacgt taattcatcg tcaaatcgtg gcgaagcgat
    17341 tctcgccgcc ctgaaaacgc agttccccgg cgcggtgctg gatgaagagc gacaaacgcc
    17401 tgaacaggtc accattacgg tgaaaatcaa tctgctgcct gacgttgtac attatcttta
    17461 ttatcaacat gatggctggc ttccagtcct gtttggcaac gacgagcgga cacttaacgg
    17521 tcattacgcg gtttattatg ccctttcaat ggaaggggcc gaaaaatgct ggatcgtggt
    17581 gaaggcactg gtcgatgccg acagtcggga gtttccgtca gtcacaccgc gcgtccctgc
    17641 cgcggtctgg ggcgagcgag aaattcgcga tatgtacggg ctgattccgg ttggcctgcc
    17701 ggatcagcgt cgcctggtgt tgcccgatga ctggccggaa gatatgcatc cgctgcgcaa
    17761 agatgcgatg gattatcgac tgcgccctga accgacgaca gattccgaaa cgtatgcgtt
    17821 tatcaatgag ggcaacagcg atgcgcgggt gatccctgtc ggcccgctgc atatcacctc
    17881 ctatgaaccg ggtcacttcc gcttgtttgt ggatggcgag caaattgtcg atgctgatta
    17941 ccgcctgttt tatgtccatc gcggcatgga gaaactggca gaaacgcgga tgggctacaa
    18001 cgaagtgacc ttcttatcgg accgcgtgtg tgggatttgc ggttttgccc acagtgtggc
    18061 ctataccaac tcggttgaaa atgcactggg gattgaggtg ccgcaacgag cacatactat
    18121 tcgctcgatt ctgctggaag tcgagcggct acacagtcat ttgctcaacc ttggcctctc
    18181 ctgccatttt gttggttttg ataccggctt tatgcaattt ttccgcgtgc gggaaaagtc
    18241 gatgacgatg gcggaattgc tgaccgggtc gcgtaaaacc tacggtctga atctgattgg
    18301 tggtgttcgc cgcgatattc tcaaagagca acgtctgcaa acgctgaaac tggtgcgcga
    18361 gatgcgcgcc gacgtgtcgg agctggtaga gatgctgctt gctacgccga atatggaaca
    18421 acgcactcag ggcattggca ttctcgaccg acaaatcgcc cgtgattata gccctgtagg
    18481 gccgctgatc cgcgcagtgg ttttgcccgt gatttgcgct ttgatcaccc ctacgccgac
    18541 tacggcaata ttccaaaaac actgtttacc tttaccggcg gcgatgtttt ctcccgcgtg
    18601 atggtccgtg tcaaagagac gtttgattcg ctggcaatgc tggaatttgc cctcgacaac
    18661 atgccggata ccccactgct gaccgaaggc tttagctata aacctcacgc attcgcgctg
    18721 ggctttgttg aagcgccacg cggtgaagac gtgcactgga gcatgctcgg tgataaccaa
    18781 aaattgttcc gctggcgctg tcgtgccgcc acctacgcca actggccggt gttgcgttac
    18841 atgctgcgcg gcaataccgt ttctgacgca ccgctgatta tcggtagcct tgatccctgc
    18901 tactcctgta ccgaccgtgt gacgctggta gatgtgcgca agcgccagtc aaaaaccgtg
    18961 ctgtataaag agatcgaacg ctacggcatt gatcgtaacc gttcgccgct gaagtaagga
    19021 cagaagatgc tgaagttact gaaaactatt atgcgcgccg gaaccgcgac ggtgaaatat
    19081 cccttcgcgc cactggaggt cagcccaggc tttcgcggga aaccggacct gatgcccagc
    19141 caatgtattg cctgcggtgc ctgcgcctgt gcttgtccgg caaatgcgct gactatccag
    19201 accgacgacc agcaaaattc gcgcacctgg cagctctatc tggggcgttg tatttactgc
    19261 ggacgttgtg aagaagtgtg cccgaccaga gccatccagc ttaccaataa ctttgaactg
    19321 accgtcaccc ataaagccga tctctatacc cacgcgacgt tccatctaca acgttgcagc
    19381 cgttgcgaac gcccgtttgc cccgcaaaaa accgtcgcac tggctgctga attgttggca
    19441 cagcaacaaa atgctccaca aaaccgcgaa atgttatggg cgcaagcgag cgtctgcccg
    19501 gagtgcaaac aacgcgcaac gctgatcaac gacgatacag atgtaccgct ggtggctaag
    19561 gagcagctat gagtccagtg cttacgcaat atgtcagcca gcccatcacg ctggacgagc
    19621 aaacgcaaaa gatgaagcgg catttgctac aggatatccg tcgctcggct tacgtttatc
    19681 gcatcgattg cggcggctgc aacgcctgtg aaatcgaaat ttttgctgcc atcacccccg
    19741 tattcgacgc agaacgtttt ggcattaagg ttgtttcatc accgcgtcac gccgatattt
    19801 tgttatttac tggcgcagtc acccgggcga tgcgtatgcc tgcacttcgg gcgtatgagt
    19861 ctgcccccga tcataaaatc tgtgtttcct acggcgcgtg cggtgtcggc ggcggtattt
    19921 tccacgatct ctacagcgtc tggggcggca gcgacaccat tgtccccatt gatgtttgga
    19981 tccccggctg cccgccaaca ccggccgcca ccattcacgg tttcgccgtg gcgctcggtt
    20041 tgctgcaaca gaagattcac gcggtggatt atcgcgatcc caccggggtg actatgcaac
    20101 cgttgtggcc gcagatcccg ccatcacagc gtatcgccat tgagcgagaa gcgcggcggc
    20161 tggcgggcta tcgtcagggg cgagaaattt gcgatcggct cctgcgccat ttaagcgacg
    20221 atcctacagg aaatcgggtt aacacctggt tgcgcgatgc cgacgatcca cgtctcaata
    20281 gtatcgttca gcaactcttt cgcgtactcc gggggttaca tgactgaaga gtgcggggaa
    20341 attgttttct ggacgctgcg aaaaaagttt gtcgccagta gcgacgagat gccggaacac
    20401 agctctcagg taatgtatta ctcgctggct atcggtcatc acgttggcgt gattgattgt
    20461 ctgaatgtcg ccttccgctg cccactgacg gaatacgaag attggcttgc actggtcgaa
    20521 gaggagcaag cccgacgtaa gatgctgggg gtgatgactt ttggtgagat tgttattgac
    20581 gccagccaca ccgccctgtt gacccgggca ttcgcgccac tggcagatga cgcgacgtct
    20641 gtgtggcaag cgcgtagcat tcaattcatt catctgttgg atgaaattgt gctggaaccg
    20701 gctatctatc tgatggccag aaaaattgcg tgagaaggat ttctcattaa taaggactgt
    20761 tgatggctat gtcagacgaa gcgatgtttg ccccgccgca aggaataaca attgaagcgg
    20821 taaacggaat gctcgcggag cggttagcgc agaaacacgg caaggcgtct ttattacgcg
    20881 ccttcatccc gctgccgccg ccgttcagcc cggtacaact tattgaactg catgttctca
    20941 aaagcaactt ctattaccgc taccatgatg atggcagcga tgtgacggca acaacagagt
    21001 atcagggcga gatggtcgat tattcgcgtt acgccgtcct tctcggcagt agtggaatgg
    21061 cggagctacg ctttattcgt acccacggca gccgttttac tccccaggat tgcacactgt
    21121 ttaactggct ggcacgcatt atcaccccgg ttctgcaatc atggctcaat gatgaagcac
    21181 agcaggtggc gctgcgtttg ctggagaaag atcgcgatca tcatcgggta ctggtggata
    21241 tcactaatgc agtgctgtca catcttgatc tcgacgatct gatcgctgac gtcgctcgtg
    21301 agatccatca ttttttcggt ctggcttcag tcagtatggt actgggcgat catcgaaaga
    21361 acgagaagtt cagcctgtgg tgcagcgatc tttctgcctc acattgtgcg tgtctgccac
    21421 acaatatgcc tggcgacagt gtattgctga cacaaacgct acaaacccga caaccgacct
    21481 tgacgcaccg tgcagatgat ctgtttctct ggcaacgcga cccgttatta ctcttacttg
    21541 catctaacgg ctgcgaatct gcgctcctta taccgcttac ctttggcaac catacaccgg
    21601 gtgcattgtt gctggcgcat acctcttcca ctctctttag tgaggaaaac tgccagctac
    21661 tacaacacat agccgatcgc atcgctattg ccgttggcaa tgccgatgcc tgccgtagaa
    21721 tgaccgattt gcaggaaagt ttgcagcaag aaaaccacca gcttagcgag cagctccttt
    21781 cgaatctggg cgtcggtgac attatctatc aaagccaggc aatggaagac ctgctccagc
    21841 aggtagatat tgtggcgaag agcgacagta cggtgttgat ttgcggtgaa accggaaccg
    21901 gcaaagaggt gatcgccaga gcgatccatc aacttagccc gcgacacgac aagccgctgg
    21961 tcaaaatcaa ctgcgctgcc atccccgcca gtcttctgga aagtgagtta ttcggtcatg
    22021 acaaaggggc gtttactggt gcgattaata cccatcgtgg tcgttttgaa attgccgatg
    22081 gcggcacgtt gtttctcgat gaaattggcg atctgccgtt agaacttcag cctaaactgc
    22141 tgcgcgtatt gcaggaacgg gagattgagc gtctcggcgg gagtagaacg atcccggtaa
    22201 atgtcagagt cattgccgcc accaaccgtg atttgtggca aatggttgaa gatcgccagt
    22261 ttcgcagcga tctcttttat cgcctgaatg tcttcccact ggaattgcct ccgctgcgcg
    22321 accgtccgga agatatccct cttttagcaa agcatttcac gcaaaaaatg gcgcgccata
    22381 tgaatcgttc aattgacgcc atcccgaccg aggcgctacg ccagttgatg tcgtgggact
    22441 ggccgggcaa cgtgcgcgag ctggaaaacg tgattgagcg agcggtactg ctgactcgcg
    22501 gtaacagtct gaatttacat cttaatgtcc gacaaagccg tttactgccg acgctaaatg
    22561 aagattcagc gcttcgcagt tcaatggcgt agttgctgca cccgacgacg ccagagaatg
    22621 acgaagaaga acgtcagcgc attgttcagg tattgcgaga aaccaatggc attgttgccg
    22681 ggccccgtgg cgcggcgaca cgattaggga tgaagcgcac cacgctgctg tcacgaatgc
    22741 agcgtctggg aatctcggtt cgcgaggtgt tgtaatctgc ttttgcagga gtatgcatga
    22801 gaaacaaact ctctttcgac ttgcagttga gcgccagaaa agcggcaatc gctgaacgga
    22861 ttgccgccca taaaattgcc cgcagtaaag tgtcggtctt tttaatggcg atgtccgctg
    22921 gcgtgtttat ggcgatcgga tttacttttt acctttccgt tatcgccgat gccccgtctt
    22981 cataggcatt aacccatctg gtgggcggcc tttgctttac acttggcttt attttgctgg
    23041 cggtttgcgg caccagcctg ttcacctcgt cggtaatgac ggtaatggca aaaagtcggg
    23101 gcgttattag ttggcgaact tggctgatta acgcacttct ggtggcctgc ggtaatctgg
    23161 caggtattgc ctgtttcagt ttgttaatct ggttttccgg gctggtgatg agtgaaaacg
    23221 cgatgtgggg agtcgcgatt ttacactgcg ccgagggcaa aatgcatcat acatttactg
    23281 aatctgtcag cctcggcatt atgtgcaatc tgatggtttg cctggcgctg tggatgagtt
    23341 attgcggacg ttcgttatgc gacaaaatcg tcgccatgat tttgtccatc accctgtttg
    23401 tcgccagtgg ctttgagcac tgtatcgcca atttgtttgt gattccgttc gccattgcca
    23461 ttcgccattt cgcccctacc tccttctggc aactggcgca cagtagcgca gacaattttc
    23521 cggcactgac ggtcagccat tttattaccg ccaatctgct cccggtgatg ctgggtaata
    23581 ttatcggcgg tgcagtgctg gtgagtattt gttatcgggc tatttattta cgtcaggaat
    23641 cctgaaaaat cagcccggcg aaacagttcg tcgggctgaa gattattctt gcgcgatttg
    23701 cccatcgggc caggcgtgga tcacggcttt gatcagcgtc gccaatggaa tggcgaagaa
    23761 tacgccccag aatccccaca aaccaccgaa gatcaccacc gataaaataa tcaccagcgg
    23821 atgcaggtta accgcttcgg agaacaacac cggtactagc aggttgccgt ccagcgcctg
    23881 aataatcaga tacaccgcga agcagctcca gaattcagta cctgcgccaa actggaatag
    23941 cgccacgcca accaccggaa tggtcaccac aaatgcgcca atgtacggaa taagaaccga
    24001 gaagccgacc agcactgcca gcagcagcga atagttcagc ccgaagagca agaaccccag
    24061 ccaggtggcg atccccacca cgatcatctc cagcactttg ccgcggatat agttggtgat
    24121 ttgttgattc atctccttcc acacctgtcc tgccagccca cggttacgcg gcagcacccg
    24181 gcgaacggcg ttcagcatct gctctttgtc tttcagcagg aagaagacca tcaatggcac
    24241 cagcaccaga tagacggcta tggtcagcaa accgaccagc gaggcgaggg aaattttcac
    24301 caccgaatcg cccatggtca gcatccgact gcgcatattt tcggccattg catcaataat
    24361 gcccgcgtcc attaacaccg gatagcggcg cggcaacgtg gcggcaaagt cagaaagctt
    24421 attgagcatc cccggcatat cgcggattaa gtagatgccc tgttgccagg cgataggcag
    24481 taccacgaac gccatcagta gcaatatacc gacaaaaacc accaatacaa tcgacgtcgc
    24541 ccagcggcgg gagcagccaa tagattgcag gcgcacggtt ggccattcca gcaaataagc
    24601 cagcacaata gccaccagca acggagcaag caggccacta aagaaaaaga taatgccaaa
    24661 tccggcaact aaaataacca gcaaggcaat cgcttccggg tcgctaaaac ggcggcgata
    24721 ccattgcatc aacatttcga gcataaagac cttccctgaa cctcaagagc gggattgcga
    24781 tccgcaattg tatcgaaatg tcacaaaaaa gacttcgctt tttatgacgg attcaggaaa
    24841 ctgaaaagtc atttgagtgg gctaatcttc gccgttacac tcaaaggcgg cgaggtggga
    24901 acgatatttc acagtatcgg tcaaatgact atcttcagaa atacaggata gaggttatgt
    24961 tcaggcagtt gaaaaaaaac ctggttgcaa ccctcattgc tgctatgacc attggtcagg
    25021 tagccccggc gtttgccgac agcgcagaca ccttgccgga tatgggaacc tccgcaggaa
    25081 gcacgctttc cattggtcag gaaatgcaga tgggcgacta ttatgtccgc cagctacgcg
    25141 gcagcgcgcc gttaattaat gacccgctgt taacgcaata tattaattcg ctggggatgc
    25201 gtctggtttc gcatgccaat tcggttaaga caccgtttca tttttttctg atcaacaacg
    25261 acgaaattaa cgcctttgct ttctttggcg gcaacgtggt gctgcactct gccctgttcc
    25321 gttattccga taacgaaagt caactggctt cagttatggc gcacgaaatc tcccacgtca
    25381 cccaacgtca cctggcgcga gcgatggaag atcaacaacg cagcgcgccg ctgacctggg
    25441 tcggcgcgtt aggttctatt ttactggcga tggccagtcc gcaggcgggg atggcggcgc
    25501 tgaccggtac actggcggga acgcgccagg ggatgatcag tttcacccag caaaatgaac
    25561 aggaagcgga ccgcattggt attcaggtgc tgcaacgctc gggattcgat ccacaggcga
    25621 tgccgacctt cctggaaaaa ttgctcgatc aggcgcgtta ctcctcgcgc ccgccggaaa
    25681 ttttattgac tcacccgttg ccggaaagtc gtctggcaga tgcccgcaac cgtgctaatc
    25741 agatgcgccc gatggtggtg cagtcgtcgg aagatttcta tctggcgaaa gcgcgcacac
    25801 tggggatgta taattccgga cgtaaccagc tcaccagtga tttgctggat gaatgggcga
    25861 aaggaaacgt tcgtcagcaa cgagcggcgc aatatggtcg tgctttacag gcgatggaag
    25921 ccaataaata cgacgaggcg cgtaaaacgc tgcaaccgtt actggcggca gaacctggca
    25981 acgcatggta tctcgatctg gctaccgata tcgatcttgg gcaaaacaaa gccaatgagg
    26041 caatcaatcg cctgaaaaat gcccgcgatt tacgcaccaa tccggtgttg cagctcaacc
    26101 tggcgaacgc ttatctgcaa ggcggtcaac cacaagaagc ggccaatatt ctgaatcgct
    26161 acacctttaa taataaagat gacagcaacg gctgggattt gctggcacag gcggaagccg
    26221 cgctaaataa ccgcgatcag gagctggctg cgcgagcaga aggttatgcg ctcgccggac
    26281 gactcgatca ggccatttcg ctgttgagta gcgccagttc gcaggtgaaa ttaggcagcc
    26341 tgcaacaagc gcgttacgat gcgcgcatcg accagttgcg ccagctgcag aaacgcttta
    26401 agccttatac caagatgtaa ttcgcattaa aggaagaaca atgaccaaac aggtaaaaat
    26461 ctaccataac ccacgctgtt caaagagccg ggaaacgctg aacctgctga aagaaaacgg
    26521 cgtggagcca gaagtggtgc tctaccttga gacacccgcc gatgcggcaa cgctgcgcga
    26581 tttgctgaaa atgctgggga tgaatagcgc ccgtgaattg atgcgccaga aagaggatct
    26641 ttataaagaa ctaaacctgg ctgacagttc gcttagcgaa gaggcgctga ttcaggcaat
    26701 ggtggataat ccgaagctga tggagcgccc gattatggtc gcgaatggca aagcgcggat
    26761 tggtcgtcca ccggagcagg tactggaaat cgtgggttaa gatgtgctcc atcgctagtt
    26821 gaagcacatt gccggatgcg acgcttgcca cgtcttatcc ggcctacgaa tactatggat
    26881 taacttcgcg taggtcaggc gttcgcgccg catccgacaa taaacacctt atctacaact
    26941 tcagaatttc tttcacaaac ggaatggtca gcttacgttg cgcggtaatc gacgcacgat
    27001 ccaactgatc caacgtcata aatagcgtgc gcatttctct gtcgagccgc ttcagcaaga
    27061 aacgccccac atcttccggc agttcaaaac cacgcaaacg cgcgcgtaac tgtagcgcct
    27121 gcaacttatc ttcatcagaa agtggctgca atttgtagat ctgcccccag tcgagacgcg
    27181 acgcgagatc cggtaatccc agattcaact gccgcggtgg acgatcgccg gtgatcaaca
    27241 accgtgtttt gcccgattcc agaattcgat tgtagagatc gaaaatcgcc atctcccaca
    27301 actcatcgcc tgcaatacac tcaatgttgt cgatacagac cagcgacaaa tgctccatac
    27361 cgtcgagcac ttccggaaca aaccaggtgc gtttatccag cgggacatag cccaccgcat
    27421 cgccacgctg cgacaattcc gcgcaagccg cgtgcagcag atggctgcgc cccgcgcctt
    27481 cgcgtgccca gagatagatg taaccgctat gttcctgacg cagcacgttt tgcagcgcgg
    27541 ccagtaaaga ggagttatcc cccggccaga aacttgcaaa ggtttcgtcg tcaggaagat
    27601 aaagtggcaa agagagctgt gccggtgtgt tcagagatac ctcaaccagg atttcacaaa
    27661 atcgcgagaa gtttaccaca gaatcccatg atgtttgaac cgggcagcaa cactgcccgg
    27721 tcggtacatt atttgtctgt tatatccgcg tcttctgcgt ccagcaccac ttcttcaggg
    27781 cgcagcacac tgatcagttt gaaaatcaaa cttaagccga taccgacgat ggtcgccagc
    27841 gccatacctt ttaactccgc cgcgccgatg tttaccttcg cgccactgac gccgatgatc
    27901 aaaatcacgg aagtcaggat caggttctgt gctttgttgt aatccacttt cgattcgatc
    27961 aaaacacgaa taccggaagc accaatgaca ccataaagca gcagcgaaac gccgcccatc
    28021 accggcaatg ggatcatctg gatagcggca gccagtttac cgacgcagga aagcaggata
    28081 gcgaaaatcg ccgccccgcc gataacccag gtactgtaaa cacgggtgat cgccatcacg
    28141 ccaatgtttt ctccgtaagt ggtatttggc gtagagccaa agaagccgga gatcacggtc
    28201 gacaggccgt tagcaaacat cgaacggtgc agacctggat cgcgcagcag atcttttttg
    28261 acgatattag ccgttactac caggtgccct acgtgttcgg caataaccac taacgccgct
    28321 ggcagaatag tcagaatggc aaaccactcg aagcgcggcg tatagagggt tggcagtgca
    28381 aaccagtgag cattaataat cggcgtggta tcgacaattc ccattgcgaa agagagcgcg
    28441 taccccacca gcacgccaat taaaatcggg ataattgcca ggaaaccacg aaacagcacg
    28501 gaacctaaaa ccgtgaccgc cagggtggta atagagatga tgatggtttt ggagtttggc
    28561 gtttgccctt cagccgggag taaacccgcc ataccggcag ctacgcccgc cagctccaga
    28621 ccgatgacgg caacgattgc gcccattgcc gcaggtggaa acagcacgtc caaccagccg
    28681 gtccccgctt tcttcacgat aaaagaaacc aggcagaaca gcacgccgca cataataaag
    28741 ccgcccagcg cgacttcata ccctaacggc aacagtaaca ataccggtga aataaaggca
    28801 aagctggaac caagataagc cggaattttc cctttacaga tgaagagata cagcagcgtt
    28861 ccaataccgt taaataacag tacagtcgcc gggttaatat gaaataagac gggcaccagg
    28921 acggttgcac caaacatggc gaacaaatgt tgcaaactaa gcgggattgt ctgtaaaagt
    28981 ggcggtcttt cactcacccc gatagcacgg cgcgtcatag tattatcctc tgtattatgt
    29041 gttataggcg ctttactcaa aaaaaagccg actcttaaag tcggctttaa ttatttttat
    29101 tctttatttc gtaccaaaga ttttgtcacc ggcatcgccg aggcccggaa taatgtatcc
    29161 gtgctcgttc agtccctggt caatcgatgc agtatacagt tcgacgtccg ggtgcgcttt
    29221 ttccagcgca gcgatacctt ctggcgcagc gaccagcacc agaactttga tgctgctgca
    29281 gcccgctttt ttcagcaggt cgatggtcgc gataacggaa ccaccggttg ccagcattgg
    29341 gtcaacgatc agcgccatac gctcatcgat gttagaaacc agtttctgga agtacggtac
    29401 cggctccagc gtttcttcat tacggtacat accgacaacg ctgatgcgcg cgctcggaac
    29461 gttttccagc acaccgtcca tcataccaag acccgcacgc agaattggca caacggtaat
    29521 tttcttacct ttgatctggt cgatttctac cgggccgttc cagccttcga tagttacttt
    29581 ttccgtttcg aggtcggcgg tcgcttcgta agtcagcagg ctacccactt cggaagcgag
    29641 ttcgcgaaag cgcttggtgc tgatatcttg ctcacgcatc agtcccagct tgtgtttgac
    29701 gagtgggtgt ttgacttcca cgatcttcat actctttctc ctttgagggg cagccacaaa
    29761 aaaatcgacg gattatacct cctttcttca gggcggcaat attattttcg ttgactttag
    29821 tcaaaatgat aacagtttga gataaagtta ttttatattc aaatggttat gaaagaagat
    29881 tattccatcc gaaaactaac ctttgccctg gcacaagtct tctttcgccg cgcgcccggg
    29941 gaaaagacgt gcaaaaaggt tgtgtaaaac agtctcgcaa acgtttgctt tccctgttag
    30001 aattgcgccg aattttattt ttctaccgca agtaacgcgt ggggacccaa gcagtgaccg
    30061 ataaaacctc tcttagctac aaagatgccg gtgttgatat tgacgcgggt aatgctctgg
    30121 ttggaagaat caaaggcgta gtgaagaaaa cgcgtcgtcc ggaagtgatg ggcggtctgg
    30181 gcggcttcgg tgcgctgtgt gcattgccgc aaaaatatcg tgaacccgtg ctggtttccg
    30241 gcactgacgg cgtaggtacc aagctgcgtc tggcaatgga cttaaaacgt cacgacacca
    30301 ttggtattga tctggtcgcc atgtgcgtta atgacctggt ggtgcaaggt gcggaaccgc
    30361 tgtttttcct cgactattac gcaaccggaa aactggatgt tgataccgct tcagcggtga
    30421 tcagcggcat cgcggaaggt tgtctgcaat cgggctgttc tctggtgggt ggcgaaacgg
    30481 cagaaatgcc ggggatgtat cacggtgaag attacgatgt cgcgggtttc tgcgtgggcg
    30541 tggtagaaaa atcagaaatc atcgacggct ctaaagtcag cgacggcgat gtgctgattg
    30601 cactcggttc cagcggtccg cactcgaacg gctattcgct ggtgcgcaaa attcttgaag
    30661 tcagcggttg tgatccgcaa accaccgaac ttgatggtaa gccattagcc gatcatctgc
    30721 tggcaccgac ccgcatttac gtgaagtcag tgctggagtt gattgaaaag gtcgatgtgc
    30781 atgccattgc gcacctgacc ggcggcggct tctgggaaaa cattccgcgc gtattgccag
    30841 ataataccca ggcagtgatt gatgaatctt cctggcagtg gccggaagtg ttcaactggc
    30901 tgcaaacggc aggtaacgtt gagcgccatg aaatgtatcg caccttcaac tgcggcgtcg
    30961 ggatgattat cgccctgcct gctccggaag tggacaaagc cctcgccctg ctaaatgcca
    31021 acggtgaaaa cgcgtggaaa atcggtatca tcaaagcctc tgattccgaa caacgcgtgg
    31081 ttatcgaata atgaatattg tggtgcttat ttccggcaac ggaagtaatt tacaggcaat
    31141 tattgacgcc tgtaaaacca acaaaattaa aggcaccgta cgggcagttt tcagcaataa
    31201 ggccgacgcg ttcggccttg aacgcgcccg ccaggcgggt attgcaacgc atacgctcat
    31261 cgccagcgcg tttgacagtc gtgaagccta tgaccgggag ttgattcatg aaatcgacat
    31321 gtacgcaccc gatgtggtcg tgctggctgg ttttatgcgc attctcagcc cggcgtttgt
    31381 ctcccactat gccgggcgtt tgctgaacat tcacccttct ctgctgccga aatatcccgg
    31441 attacacacc catcgtcagg cgctggaaaa tggcgatgaa gagcacggta catcggtgca
    31501 tttcgtcacc gatgaactgg acggtggccc ggttatttta caggcgaaag tcccggtatt
    31561 tgctggtgat acggaagatg acatcaccgc ccgcgtgcaa acccaggaac acgctattta
    31621 tccactggtg attagctggt ttgccgatgg tcgtctgaaa atgcacgaaa acgccgcgtg
    31681 gctggatggt caacgtctgc cgccgcaggg ctacgctgcc gacgagtaat gcccccgtag
    31741 ttaaagcgcc agctctgccg ctggcgtttt tcaattcacc tgtaaatcgc aagctccagc
    31801 agtttttttc ccccttttct ggcatagttg gacatctgcc aatattgctc gccataatat
    31861 ccaggcagtg tcccgtgaat aaaacggagt aaaagtggta atgggtcagg aaaagctata
    31921 catcgaaaaa gagctcagtt ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga
    31981 caaatctaac ccgctgattg aaaggatgcg tttcctgggg atctattcca ataaccttga
    32041 tgagttctat aaagtccgct tcgctgaact gaagcgacgc atcattatta gcgaagaaca
    32101 aggctccaac gctcattccc gccatttact gggcaaaatt cagtcccggg tgctgaaagc
    32161 cgatcaggaa ttcgacggcc tctacaacga gctattgctg gagatggcgc gcaaccagat
    32221 cttcctgatt aatgaacgcc agctctccgt caatcaacaa aactggctgc gtcattattt
    32281 taagcagtat ctgcgtcagc acattacgcc gattttaatc aatcctgaca ctgacttagt
    32341 gcagttcctg aaagatgatt acacctatct ggcggtggaa attatccgta gcgatagcat
    32401 ccgttacgcg ctgctggaga tcccatcaga taaagtgccg cgctttgtga atttaccgcc
    32461 agaagcgccg cgtcgacgca agccgatgat tcttctggat aacattctgc gttactgcct
    32521 tgatgatatt ttcaaaggct tctttgatta tgacgcgctg aatgcctatt caatgaagat
    32581 gacccgcgat gccgaatacg atttagtgca tgagatggaa gccagcctga tggagttgat
    32641 gtcttccagt ctcaagcagc gtttaactgc tgagccggtg cgttttgttt atcagcgcga
    32701 tatgcccaat gcgctggttg aagtgttacg cgaaaaactg actatttccc gctacgactc
    32761 catcgtcccc ggcggtcgtt atcataattt taaagacttt attaatttcc ccaatgtcgg
    32821 caaagccaat ctggtgaaca aaccactgcc gcgtttacgc catatttggt ttgataaagc
    32881 ccagttccgc aatggttttg atgccattcg cgaacgcgat gtgttgctct attatcctta
    32941 tcacaccttt gagcatgtgc tggaactgct gcgtcaggct tcgttcgacc cgagcgtact
    33001 ggcgattaaa attaacattt accgcgtggc gaaagattca cgcatcatcg actcgatgat
    33061 ccacgccgca cataacggta agaaagtcac tgtggtggtt gagttacagg cgcgtttcga
    33121 cgaagaagcc aacattcact gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt
    33181 ctctgcgccg gggctgaaaa ttcacgccaa actgttcctg atttcacgta aagaaaacgg
    33241 tgaagtggtc cgttacgcac acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct
    33301 ttatactgac tattcgttgc tgaccgcaga tgcgcgtatc accaacgaag tacggcgggt
    33361 atttaacttt attgaaaacc cataccgccc ggtgacattt gattatttaa tggtgtcacc
    33421 gcaaaactct cgccgtctgt tatatgaaat ggtagaccgc gaaatcgcca acgcgcagca
    33481 agggctgccc agtggtatca ccctgaagct aaataacctt gtcgataaag gcctggttga
    33541 tcgtctgtat gcggcctcca gctccggcgt accggttaat ctgctggttc gcggaatgtg
    33601 ttcgctgatc cccaatctgg aaggcattag cgacaacatt cgtgccatca gtattgttga
    33661 ccgttacctt gaacatgacc gggtttatat ttttgaaaat ggcggcgata aaaaggtcta
    33721 cctttcttcc gccgactgga tgacgcgcaa tattgattat cgtattgaag tggcgacacc
    33781 gctgctcgat ccgcgcctga agcagcgggt gctggacatc atcgacatat tgttcagcga
    33841 tacggtcaaa gcacgttata tcgataaaga actcagtaat cgctacgttc cccgcggcaa
    33901 tcgccgcaaa gtacgggcgc agttggcgat ttacgactac atcaaatcac tcgaacaacc
    33961 tgaataaccc tatgccaata cacgataaat cccctcgtcc gcaggagttt gctgcggtcg
    34021 atcttggttc aaacagtttt cacatggtca tagcccgtgt ggtagatggt gccatgcaga
    34081 ttattggccg cctgaaacag cgggtgcatc tggcggacgg cctggggcca gataatatgt
    34141 tgagtgaaga ggcaatgacg cgcggtttga actgtctgtc gctgtttgcc gaacggctac
    34201 aagggttttc tcctgccagc gtctgtatag tcggcaccca tacgctgcgt caggcgctga
    34261 acgccactga ctttctgaaa cgcgcggaaa aggtcattcc ctacccgatt gaaattattt
    34321 ccggtaatga agaagcccgt ctgattttta tgggcgtgga acatacccaa ccggaaaaag
    34381 gtcgcaaact ggttattgat attggcggcg gatctacgga actggtgatt ggtgaaaatt
    34441 tcgaacctat tctcgttgaa agccgccgga tgggttgtgt cagctttgcc cagctttatt
    34501 ttcctggcgg ggtcatcaat aaagagaatt ttcagcgcgc tcgcatggcg gcagcacaaa
    34561 aactggaaac tttaacctgg caattccgta ttcagggctg gaacgttgca atgggcgctt
    34621 ccggtaccat aaaagccgcc catgaagtgt taatggaaat gggcgagaaa gacgggataa
    34681 ttaccccgga acgtctggaa aaactggtaa aagaaatttt acgtcaccgt aatttcgcat
    34741 cgctgagttt accgggtctt tccgaagagc ggaaaacagt cttcgttcct ggactggcga
    34801 ttttatgtgg tgtgtttgat gctttagcca tccgtgaact gcgcctttct gacggggcgt
    34861 tacgcgaagg cgtactgtat gaaatggaag gacgtttccg tcatcaggat gtgcgtagtc
    34921 gcaccgccag cagcctcgcc aaccagtatc acatagacag cgaacaggcc cgacgagtgc
    34981 tggataccac tatgcaaatg tacgaacagt ggcgggaaca gcaaccgaag ctggcgcatc
    35041 cgcaactgga ggcgctactg cgatgggccg ccatgctgca tgaggtcggg ttgaatatca
    35101 accacagcgg tttgcatcgc cactccgctt atattctgca aaacagtgac ttgccgggtt
    35161 ttaatcagga acagcagctg atgatggcga cactggtgcg ctatcaccgt aaagcgatta
    35221 agctcgacga tctgccgcgc tttaccttgt ttaagaagaa acagttcctg ccactgatac
    35281 agctattgcg ccttggcgta ttactaaaca atcaacgtca ggcaaccacc acaccgccaa
    35341 cattgacact gattactgat gacagtcact ggacactgcg tttcccgcat gactggttta
    35401 gtcagaatgc gctggtactg cttgatctgg aaaaggagca agaatactgg gaaggcgtgg
    35461 ctggctggcg gttgaaaatt gaagaagaaa gtaccccaga aatcgccgct taatgctttg
    35521 cgggccgacg agaaatgtcg gcccgcatta ttcaggcact ttcgcgaatg ggttcgattt
    35581 cattcagcgt atcaattaac ggttgcggct taccaataag ataaccctgc atataatcga
    35641 tccccaaaga gagcaccgcc tcgcggatct cttcgttttc aacgtactct gccactaccc
    35701 gcattttctt cattcgcgcc agatggcaaa tcgacgccac aatctgataa tccagactat
    35761 ttgacacaat attgctgata aagctgccgt caattttaag cagatcggca ttcacatttt
    35821 taagccgtgc atagctggcg tagccggtgc cgaaatcatc aatcgcaatc tggcagccta
    35881 attcctgaag atgctgcaag gtgatttgcg cctgcttaac attggtcaga gcattacttt
    35941 cggtgacttc aaaaataagt tgccacgctt caatctgata tttagccagc aactgactga
    36001 cttcaacagg aaaacgagcc tgacataccg aggttggaga cagattaata gcaaaacggt
    36061 gagcgggcat cttcgctctg ttttcagcca taaattgcag cgtatgctca atgacccaca
    36121 tgtcgatact cgacgataaa ccaaattcgt gcgcgaccgg caagaagcta ccggggccga
    36181 tcagttcatc attctcacct ttcatgcgca gaagaatttc atggtaaaca tcaccacgca
    36241 taccggtaat cggctgggcc atcaggaaaa aatggttgtg ttccagcgcc tgctgtagtc
    36301 gattcatcat cgcgacttta tctttcaatt cgcgttgcaa atacattgcc ccgcaacgct
    36361 gcatattttc cggggcgttg gtcacgatgg aaagttcggc gaccgtattt agctctccca
    36421 gcagcaggta gatatgattc actggcgagc gcacatagca gtaactgacg ccaatctgcg
    36481 gttgcatcgg catcccatcc caaaagaaac gaaattgctt gagatgacta tccagtgcgg
    36541 taatgcgctc ctggtgcgat tctgtattca gtcgcagcgc gagatcgtta cccgaaagct
    36601 gataaacatc ttcacccggt tccagcaagg gtgacagcca gtgagaaagt ttttgctt
//
LOCUS       DAZXUK010000011        36587 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZXUK010000011 DAZXUK010000000
VERSION     DAZXUK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36587
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.011"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..190
                     /locus_tag="ACH3L8_000471"
     CDS             <1..190
                     /locus_tag="ACH3L8_000471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4083735.1"
                     /translation="PPHVKALPDTITQTSIHRTLQFIEFARSCRPDKAFTPHPAINRR
                     RLPNGRFFTYSPPSNVPA"
     gene            complement(156..1391)
                     /locus_tag="ACH3L8_000472"
     CDS             complement(156..1391)
                     /locus_tag="ACH3L8_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFT4083736.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="ACH3L8_000473"
     tRNA            complement(1538..1614)
                     /locus_tag="ACH3L8_000473"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="ACH3L8_000474"
     tRNA            complement(1657..1743)
                     /locus_tag="ACH3L8_000474"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="ACH3L8_000475"
     tRNA            complement(1764..1839)
                     /locus_tag="ACH3L8_000475"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="ACH3L8_000476"
     tRNA            complement(1898..1974)
                     /locus_tag="ACH3L8_000476"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="ACH3L8_000477"
     CDS             complement(2077..3462)
                     /locus_tag="ACH3L8_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFT4083737.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACH3L8_000478"
     CDS             complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACH3L8_000478"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFT4083738.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACH3L8_000479"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACH3L8_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFT4083739.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACH3L8_000480"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACH3L8_000480"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFT4083740.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACH3L8_000481"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACH3L8_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFT4083741.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACH3L8_000482"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACH3L8_000482"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFT4083742.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACH3L8_000483"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACH3L8_000483"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFT4083743.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACH3L8_000484"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACH3L8_000484"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFT4083744.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACH3L8_000485"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACH3L8_000485"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFT4083745.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACH3L8_000486"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACH3L8_000486"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFT4083746.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACH3L8_000487"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACH3L8_000487"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFT4083747.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACH3L8_000488"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACH3L8_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFT4083748.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACH3L8_000489"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACH3L8_000489"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFT4083749.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACH3L8_000490"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACH3L8_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFT4083750.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACH3L8_000491"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACH3L8_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFT4083751.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACH3L8_000492"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACH3L8_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFT4083752.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="ACH3L8_000493"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="ACH3L8_000493"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFT4083753.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="ACH3L8_000494"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="ACH3L8_000494"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFT4083754.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACH3L8_000495"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACH3L8_000495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFT4083755.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="ACH3L8_000496"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="ACH3L8_000496"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFT4083756.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACH3L8_000497"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACH3L8_000497"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFT4083757.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="ACH3L8_000498"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="ACH3L8_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFT4083758.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACH3L8_000499"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACH3L8_000499"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFT4083759.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACH3L8_000500"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACH3L8_000500"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFT4083760.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACH3L8_000501"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACH3L8_000501"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFT4083761.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACH3L8_000502"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACH3L8_000502"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFT4083762.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACH3L8_000503"
     CDS             complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACH3L8_000503"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFT4083763.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACH3L8_000504"
     CDS             complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACH3L8_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFT4083764.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACH3L8_000505"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACH3L8_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFT4083765.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="ACH3L8_000506"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="ACH3L8_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACH3L8_000507"
     CDS             complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACH3L8_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFT4083766.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35463..36302
                     /gene="hdfR"
                     /locus_tag="ACH3L8_000508"
     CDS             35463..36302
                     /gene="hdfR"
                     /locus_tag="ACH3L8_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFT4083767.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36398..36473)
                     /locus_tag="ACH3L8_000509"
     tRNA            complement(36398..36473)
                     /locus_tag="ACH3L8_000509"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36438..36440),aa:Trp,seq:cca)
     gene            complement(36482..36558)
                     /locus_tag="ACH3L8_000510"
     tRNA            complement(36482..36558)
                     /locus_tag="ACH3L8_000510"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36522..36524),aa:Asp,seq:gtc)
BASE COUNT         8942 a        10012 c         9215 g         8418 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccagacag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    35041 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    35101 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    35161 ctacaaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    35221 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    35281 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    35341 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    35401 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    35461 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    35521 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    35581 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    35641 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
    35701 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcgttgt
    35761 gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat gcccatacag
    35821 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    35881 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    35941 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
    36001 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    36061 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    36121 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc
    36181 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata
    36241 gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat gaagtgtatt
    36301 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg attatttcag
    36361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    36421 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    36481 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    36541 cgactgaact accgctccac cgaattcttt tacaaccacc ggtttta
//
LOCUS       DAZXUK010000012        35483 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZXUK010000012 DAZXUK010000000
VERSION     DAZXUK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35483
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.012"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(118..3267)
                     /gene="acrB"
                     /locus_tag="ACH3L8_000511"
     CDS             complement(118..3267)
                     /gene="acrB"
                     /locus_tag="ACH3L8_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFT4083768.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3290..4483)
                     /gene="acrA"
                     /locus_tag="ACH3L8_000512"
     CDS             complement(3290..4483)
                     /gene="acrA"
                     /locus_tag="ACH3L8_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFT4083769.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4625..5272
                     /gene="acrR"
                     /locus_tag="ACH3L8_000513"
     CDS             4625..5272
                     /gene="acrR"
                     /locus_tag="ACH3L8_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFT4083770.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5400..8762
                     /gene="mscK"
                     /locus_tag="ACH3L8_000514"
     CDS             5400..8762
                     /gene="mscK"
                     /locus_tag="ACH3L8_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFT4083771.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8974..9135)
                     /gene="rsmS"
                     /locus_tag="ACH3L8_000515"
     CDS             complement(8974..9135)
                     /gene="rsmS"
                     /locus_tag="ACH3L8_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFT4083772.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(9149..9676)
                     /gene="priC"
                     /locus_tag="ACH3L8_000516"
     CDS             complement(9149..9676)
                     /gene="priC"
                     /locus_tag="ACH3L8_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFT4083773.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9746..10123
                     /locus_tag="ACH3L8_000517"
     CDS             9746..10123
                     /locus_tag="ACH3L8_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFT4083774.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10276..10827
                     /gene="apt"
                     /locus_tag="ACH3L8_000518"
     CDS             10276..10827
                     /gene="apt"
                     /locus_tag="ACH3L8_000518"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFT4083775.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10956..12881
                     /gene="dnaX"
                     /locus_tag="ACH3L8_000519"
     CDS             10956..12881
                     /gene="dnaX"
                     /locus_tag="ACH3L8_000519"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFT4083776.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    12214..12278
                     /gene="dnaX"
                     /locus_tag="ACH3L8_000519"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12934..13263
                     /locus_tag="ACH3L8_000520"
     CDS             12934..13263
                     /locus_tag="ACH3L8_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFT4083777.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13263..13868
                     /gene="recR"
                     /locus_tag="ACH3L8_000521"
     CDS             13263..13868
                     /gene="recR"
                     /locus_tag="ACH3L8_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFT4083778.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13978..15852
                     /gene="htpG"
                     /locus_tag="ACH3L8_000522"
     CDS             13978..15852
                     /gene="htpG"
                     /locus_tag="ACH3L8_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFT4083779.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16033..16677
                     /gene="adk"
                     /locus_tag="ACH3L8_000523"
     CDS             16033..16677
                     /gene="adk"
                     /locus_tag="ACH3L8_000523"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFT4083780.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            16809..17771
                     /gene="hemH"
                     /locus_tag="ACH3L8_000524"
     CDS             16809..17771
                     /gene="hemH"
                     /locus_tag="ACH3L8_000524"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFT4083781.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17768..18727)
                     /gene="aes"
                     /locus_tag="ACH3L8_000525"
                     /pseudo
     CDS             complement(17768..18727)
                     /gene="aes"
                     /locus_tag="ACH3L8_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            18879..20183
                     /gene="gsk"
                     /locus_tag="ACH3L8_000526"
     CDS             18879..20183
                     /gene="gsk"
                     /locus_tag="ACH3L8_000526"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFT4083782.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(20316..21992)
                     /gene="ybaL"
                     /locus_tag="ACH3L8_000527"
     CDS             complement(20316..21992)
                     /gene="ybaL"
                     /locus_tag="ACH3L8_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFT4083783.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22230..23450)
                     /gene="fsr"
                     /locus_tag="ACH3L8_000528"
     CDS             complement(22230..23450)
                     /gene="fsr"
                     /locus_tag="ACH3L8_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFT4083784.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23668..25320
                     /gene="ushA"
                     /locus_tag="ACH3L8_000529"
     CDS             23668..25320
                     /gene="ushA"
                     /locus_tag="ACH3L8_000529"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFT4083785.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25357..25836)
                     /gene="ybaK"
                     /locus_tag="ACH3L8_000530"
     CDS             complement(25357..25836)
                     /gene="ybaK"
                     /locus_tag="ACH3L8_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFT4083786.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26040..26834)
                     /locus_tag="ACH3L8_000531"
     CDS             complement(26040..26834)
                     /locus_tag="ACH3L8_000531"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFT4083787.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26972..27313
                     /locus_tag="ACH3L8_000532"
     CDS             26972..27313
                     /locus_tag="ACH3L8_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFT4083788.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27528..30032)
                     /gene="copA"
                     /locus_tag="ACH3L8_000533"
     CDS             complement(27528..30032)
                     /gene="copA"
                     /locus_tag="ACH3L8_000533"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFT4083789.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30294..31226
                     /gene="glsA"
                     /locus_tag="ACH3L8_000534"
     CDS             30294..31226
                     /gene="glsA"
                     /locus_tag="ACH3L8_000534"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFT4083790.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31229..32521
                     /locus_tag="ACH3L8_000535"
     CDS             31229..32521
                     /locus_tag="ACH3L8_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFT4083791.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            32552..32649
                     /locus_tag="ACH3L8_000536"
                     /pseudo
     CDS             32552..32649
                     /locus_tag="ACH3L8_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32646..33053
                     /gene="cueR"
                     /locus_tag="ACH3L8_000537"
     CDS             32646..33053
                     /gene="cueR"
                     /locus_tag="ACH3L8_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFT4083792.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33054..33512)
                     /locus_tag="ACH3L8_000538"
     CDS             complement(33054..33512)
                     /locus_tag="ACH3L8_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFT4083793.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(33509..34426)
                     /locus_tag="ACH3L8_000539"
     CDS             complement(33509..34426)
                     /locus_tag="ACH3L8_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFT4083794.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            34572..35249
                     /gene="fetA"
                     /locus_tag="ACH3L8_000540"
     CDS             34572..35249
                     /gene="fetA"
                     /locus_tag="ACH3L8_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFT4083795.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            35236..>35483
                     /locus_tag="ACH3L8_000541"
     CDS             35236..>35483
                     /locus_tag="ACH3L8_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4083796.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQ"
BASE COUNT         8620 a         9323 c         9316 g         8224 t
ORIGIN      
        1 cgtggttaat actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg
       61 ttcgtaggtt atgcataaaa aaggccgctt acgcggcctt agtgattaca tgttgtatca
      121 atgatgatcg acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg
      181 aaccaccaca aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc
      241 gcccattaca ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac
      301 cagcggcata acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat
      361 ccgcaccgca tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt
      421 ggcgaattcg acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcagtcc
      481 tacttggaag taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat
      541 aacccccagc ggaacgacca gcataacgga gaacggagtc gaccagctct cgtacagcgc
      601 cgccagacac aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc
      661 ggagagacgt tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt
      721 gctcgccagt tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg
      781 gcctaagatt tccatggatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc
      841 ccaacgagaa gaggagaacg ccgagaatgg caccatctga ccatcagcag cacgaacata
      901 ccagtcgccg atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt
      961 cttcacacga ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag
     1021 agtggtgtta atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc
     1081 aatcttaaac tgcggggtat cttccagacc gtttggacgt acgctggtca gcatatcagg
     1141 gtgcttcgct gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag
     1201 gccagcctgg tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc
     1261 gggcaggtta aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg
     1321 catggtaatc gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga
     1381 aacgaacgca ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa
     1441 caccgactca acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac
     1501 tttctgcgta cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc
     1561 ctggtcctca tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac
     1621 cacgatgatc agatacagca ctaggtaacg ccccgtactg cgcagaatac cgcctacgct
     1681 gtcggtgtag tggtgcgtgc tcttctcgaa catgcggttc aaccagccga agaagccttt
     1741 tttaccttcc ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg
     1801 agtcaggatc aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa
     1861 ctgacgatag atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga
     1921 cagtaccatc gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc
     1981 ttcttttggc ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat
     2041 agcgtcatcc accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat
     2101 cgagaagcca aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat
     2161 ggtcggaatc aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag
     2221 gatgatcgct tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt
     2281 ggtgtcgtac gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag
     2341 ttcagcacgg attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc
     2401 cagaccggaa gcaggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag
     2461 ctcaatcttc gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag
     2521 gattttgccg aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag
     2581 ctgttggcct ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg
     2641 cgctttgatg gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg
     2701 gttcatccag atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga
     2761 cgtacggctg atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt
     2821 catggtgcca tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc
     2881 aacgctcacc ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt
     2941 gttctgcacc tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat
     3001 ctgcacggta cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt
     3061 catattctgt tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg
     3121 gtaggaggcg gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag
     3181 tttgaggatc gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat
     3241 cgggcgatcg ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg
     3301 actgttcagg ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg
     3361 cttttacctg gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg
     3421 ctttcagacc ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac
     3481 gggtttccac tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac
     3541 gggttacgcc ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg
     3601 cgcgcacgaa catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg
     3661 tgatagaccc agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg
     3721 ggaacttaat gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca
     3781 gcgtgccatt cgccagttcc tgtttcaggc gaaggaagtc gttgctggac tgggtcacat
     3841 caacgtagat cggatcaagt tgctgcacgg ttgccagcac agtcgcctga ccgttctgta
     3901 ccaatgcgcc ttccgtcacg ttcgacttac caatgcgacc gctaatcgga gaggtgactt
     3961 tggtgtaagc cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag
     4021 cattcgcctg ttgcgcatca gccagagcct gatcgtaccc ttgcttactg atgtactgag
     4081 taccgagcag tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg
     4141 ctttcgccag atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct
     4201 gatagagaga gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa
     4261 tcccgctaac ttgaggacga acttctgcga tccggtaggc actggtgcga cctggaagct
     4321 cggttgtgat ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct
     4381 ggccaccttg ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca
     4441 tcagaacgac cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt
     4501 ccgatttcaa attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc
     4561 gtcgtgctat ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga
     4621 acatatggca cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt
     4681 ggctctacgt cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa
     4741 agcagctggc gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt
     4801 cagtgagatc tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc
     4861 aaaattccct ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc
     4921 cacggtgaca gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt
     4981 tgtcggagaa atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga
     5041 ccgtatagaa caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat
     5101 gacgcgtcgg gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct
     5161 ctttgccccg caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact
     5221 ggagatgtat ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat
     5281 ctgactccag gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg
     5341 atattcttgc ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca
     5401 tgactatgtt ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt
     5461 ttgtttttgt cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc
     5521 tgccgacaaa agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt
     5581 ctgctcagga caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa
     5641 tcgatcgcat aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccggaaa
     5701 aaatgcgcca ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa
     5761 cgcgcaaaat tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc
     5821 tggacgattt gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt
     5881 tacagacgca gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac
     5941 aaattcgcag tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga
     6001 aagtgttaat gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa
     6061 gcctggaagg gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg
     6121 cgaacagcgc ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc
     6181 gcctgacttt aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta
     6241 ttcaggctaa tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc
     6301 tgattaccgc gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact
     6361 ggctggagcg ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg
     6421 gcagcctgct gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg
     6481 aactggaaaa catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc
     6541 agcagcgtga tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca
     6601 ccaacgaagt caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg
     6661 aattgctgga tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc
     6721 aaatcaacca gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc
     6781 aaatcttttg ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc
     6841 aaagcctgaa agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc
     6901 ctgccgtttt tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc
     6961 actggcgtct gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc
     7021 tgcgtaacga tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc
     7081 tgccggtgtg cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca
     7141 tcagcgaact gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc
     7201 tgtgctggaa ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc
     7261 agcagaccag ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc
     7321 atttctggtc tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc
     7381 aggcgatgat tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg
     7441 aaagctggcg tgataaagag tcgcacacca tgcgactagt caccattacc gtgctgtcga
     7501 ttatcccgat tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc
     7561 tctccgggcg ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga
     7621 cggtattgcg cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc
     7681 gtcggcagaa tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca
     7741 ttgcactgga gcaagttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc
     7801 tgttcggtgt catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg
     7861 acagcatcac gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg
     7921 tcaccatggg cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc
     7981 gcaacctgcc tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgccagggcg
     8041 cgtcgtatgc cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg
     8101 tgttcggatc gctgggcgtc tcttgggata aactccagtg gctggccgca gcattatccg
     8161 taggtcttgg ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc
     8221 tgttcgaacg tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg
     8281 taagtaagat ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca
     8341 tcccgaacaa agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta
     8401 cgcgtctggt gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag
     8461 tgttactgaa ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct
     8521 tctttacggc atttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac
     8581 tgcgtgaccg tagccgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg
     8641 aaaacgacat caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg
     8701 atgaggtgac ggaagtaaaa cgcgactaca aaggcgacga tccgacacca gcggttgggt
     8761 aataaccaaa gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc
     8821 atgccggatg cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatatatt
     8881 gcaggagcgg tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccgcaa
     8941 tcttagggga catttagcgg ccccatttat ttctcacttt tccgcctcat catcgtgcgt
     9001 taatttcttt tcataatcac gctttacaat atccagcgcg cgcagcacgg tactggcagg
     9061 gatctgattt tcctccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc
     9121 attttcaagt gacatactct ttccattgct agcgggttaa gcgcgctagc ctgttttcga
     9181 ttttttccag cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat
     9241 gcagcgtttg ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa
     9301 ccatctcacg tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg
     9361 aaattttcgg tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga
     9421 tggcttccag ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct
     9481 gctcaactgc atgacgaagc gcagccagat tatcgcccgc ctcgtcgaga caagactgta
     9541 gtgttgtcgc acgagtctga aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact
     9601 gtgacaccgg ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca
     9661 gcagggcggt tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc
     9721 actatgtaag ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct
     9781 ggcggtagtg ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat
     9841 cctgctggcg gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta
     9901 ccgctcatgg tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg
     9961 cgtcaaaccg cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt
    10021 ccagatgcca tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat
    10081 gtggcgaatt ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt
    10141 tgcaattatt gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca
    10201 aaggttaaac aacagttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca
    10261 agcaggcata cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc
    10321 aaaagcattc aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg
    10381 gaagacccga aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg
    10441 ggcattacca aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct
    10501 ctgggtctgg gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc
    10561 atcagtgaaa attacgacct ggaatacggc accgatcagc tggaaatcca cgttgatgcc
    10621 atcaaaccgg gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc
    10681 gaagcgaccg ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt
    10741 atcaacctgt ttgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac
    10801 agccttgtcc cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga
    10861 cagcacaaga tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt
    10921 cgtgaatcca ccttccagcg tttcagagcc tgccaatgag ttatcaggtt ttagcccgaa
    10981 aatggcgccc acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg
    11041 cgaacggctt gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg
    11101 tcggaaaaac ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta
    11161 ccgcgacgcc gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg
    11221 atctgattga aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg
    11281 ataacgtcca gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc
    11341 atatgctgtc gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc
    11401 acgttaagtt tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcac
    11461 gctgtctgca atttcatctc aaggcgctgg atgtcgagca aattcgccat cagcttgagc
    11521 acatcctcaa tgaagaacat atcgctcacg agccgcgggc gctgcaattg ttggcgcgcg
    11581 ccgctgaagg cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg
    11641 acggccaggt ttcaacccag gcggtcagtg cgatgctggg tacgcttgac gacgatcagg
    11701 cgctgtcgct ggttgaagcg atggtcgagg gcaacggcga gcgcgtaatg gcactgatta
    11761 atgaagccgc tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcttgt
    11821 tgcatcgtat tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca
    11881 tcgagctgcg gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact
    11941 atcagacgct gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg
    12001 ttgagatgac gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg cctgagccag
    12061 aagtgccacg ccagtccttt gcacccgtcg cgccaacggc agtaatgacg ccaaccctgg
    12121 tgccgccgca atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc
    12181 aggtgctggc ggcgcgccag cagttgcagc gcgtgcaggg agcaaccaaa gcaaaaaaga
    12241 gtgaaccggc agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg
    12301 cttcggtcac cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgccag
    12361 ccaaaaaaga agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg
    12421 tcgccacgcc gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg
    12481 tgaagctagc ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt
    12541 cgctaccaaa actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg
    12601 cagtatgtct gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa
    12661 aactggctga agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag
    12721 atgataatcc cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac
    12781 ttgcgcaggc gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggtttt
    12841 tcgatgcgga gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt
    12901 tcgtcatcct taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac
    12961 ctgatgaagc aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg
    13021 cagctggaag ttaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca
    13081 cacaactgcc gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg
    13141 gaagacctgg tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa
    13201 gaaaaaatgg cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc
    13261 tgatgcaaac cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg
    13321 ttggcccgaa gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg
    13381 ggatgcgtct ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt
    13441 gccgcacttt caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa
    13501 acggtcaaat ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc
    13561 agttttctgg tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc
    13621 cggatgatat cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag
    13681 tgatcctcgc caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc
    13741 tttgcgcgca atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg
    13801 aactggaaat ggtcgatggc accacgctgt cacactccct cgccgggcgt cataagattc
    13861 gtttttaagc aaacgagagc aggagcacct gctctcgctt gaaattattc tcccttgtcc
    13921 ccatctctcc ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg
    13981 aaaggacaag aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc
    14041 cattctctct attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat
    14101 gcggcggaca agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc
    14161 gaactgcgcg ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctccgataac
    14221 ggcgtgggga tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt
    14281 accaaatcat tcctcgaatc cctgggttct gaccaagcga aagacagcca gctgatcggt
    14341 cagtttggtg ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact
    14401 cgcgcggcag gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa
    14461 tataccgttg ccgatatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt
    14521 gaaggcgaag acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc
    14581 gaccatatcg cgctgccggt agagatcgaa aaacgcgaag agaaagatgg cgaaaccgtt
    14641 atctcctggg caaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc
    14701 accgatgaag agtataaaga gttctacaaa cacatcgccc acgactttaa cgatccgctg
    14761 acctggagcc acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacatcccg
    14821 tcccaggctc cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtt
    14881 caacgcgtgt ttatcatgga cgacgctgaa cagttcatgc cgaactatct gcgcttcgtg
    14941 cgtggtctga ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac
    15001 agcacggtaa cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa
    15061 aaactggcga aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta
    15121 ctgaaagaag gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt
    15181 tttgcttcta cttataccga ttcttctgcg cagaccgtgt ctctggaaga ctacgtttcc
    15241 cgcatgaaag aagggcagga gaaaatctac tacatcactg cagacagcta tgcggcagcg
    15301 aagagcagcc cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc
    15361 gaccgcatcg atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag
    15421 tcggtgtcta aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg
    15481 aaagaagcgg agaaagcact gactccgttc atcgaccgcg tgaaagctct gctcggcgag
    15541 cgcgtgaaag atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc
    15601 gacgcggacg aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg
    15661 ccagaagtga aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca
    15721 gatactgaag atgaagccaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg
    15781 ctggcagaac gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaatcagctg
    15841 ctggtttcct gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca
    15901 tctgtacttt ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga
    15961 ggcaatcgcc tgttggtggt atcgtttatc gctttttcaa aaaaatcgac acattttaag
    16021 gggattttcg caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag
    16081 gctcagttca tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt
    16141 gctgcggtca aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc
    16201 aaactggtca ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac
    16261 tgccgtaatg gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg
    16321 aaagaagcgg gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt
    16381 gttgatcgta tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa
    16441 ttcaatccgc cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt
    16501 aaagacgatc aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca
    16561 ccgctgatcg gctactactc caaagaagcg gaagcgggta acaccaaata cgcgaaagtt
    16621 gacggtacca agcaggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca
    16681 agccgcagca gatctgcttt cgggcaggtc tgcttatcca cctcaggcaa tacctctcac
    16741 agcaattagt tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag
    16801 aggcggtaat gcgtcagact aaaaccggta tcctgctggc aaacctgggt acgcccgatg
    16861 cccccacacc tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg
    16921 ttgatacctc acggttgtta tggtggccgt tgctgcgcgg cgtgattttg ccgctgcgct
    16981 cgccgcgtgt ggcgaagctg tacgcctctg tctggatgga agacggctcg ccgctgatgg
    17041 tttacagccg ccagcaacag caggcgttgg cacaacgttt accggatacg ccagtggcgc
    17101 tgggcatgag ctacggctcg ccatcactgg aaagctccgt cgatgaactc ctggcagagc
    17161 atgtagatca tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg
    17221 cggtatggga tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt
    17281 ttattcgtga ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg
    17341 cttcttttgc caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc
    17401 agcgttatgc agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac
    17461 tggcttccgc actggggatg gtaccggaaa aagtgatgat gacctttcag tcgcgctttg
    17521 gccgggaacc ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag
    17581 gcgtaggtca tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg
    17641 aagagattgc cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat
    17701 atattccagc gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg
    17761 cgtatcgcta aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg
    17821 ttttcatcat ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact
    17881 cacagggctg ctgatgcgcc gctaacgtct agtaaagcag acggctgtca tccagcagcg
    17941 gatcgaactc cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa
    18001 acagacagta atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca
    18061 tctgcaaatc ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg
    18121 aatcccgtaa tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga
    18181 tctgtttatc acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac
    18241 cggcaaagcc aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac
    18301 aacaagcagc cacaatttcc tctatcgctt gtggaaaacg cgcttcaggt gaaagggtgt
    18361 aattaatacc aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt
    18421 gggtatcgag attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc
    18481 tatctggctg cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt
    18541 aagctctggt tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat
    18601 actgtcgttg ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag
    18661 tattcacaac ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg
    18721 gcttcataca aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg
    18781 cgatcccgcc tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt
    18841 aacattcgcc gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta
    18901 aatccaaaca ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag
    18961 aaaacgaaac cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag
    19021 cgaaagtgga tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga
    19081 ttgaggatga cgtagccgaa gtgctttatc aggagctaaa acagaaaaac ctgattaccc
    19141 atcagtttgc gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg
    19201 accgttcggt gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc
    19261 gttacctgtg taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggtc
    19321 cgattggtcg ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc
    19381 caggccacat gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct
    19441 cggcactggt tctcacctca tatctggtgc gttgcaagcc gggtgaaccc atgccggaag
    19501 caaccatgaa agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg
    19561 gcaccaagtt tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagaccacg
    19621 tttctatcct tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt
    19681 tgttggcatc tgacaaggcg ctggactggg tagagctggt gctgtgcacc gccgggccaa
    19741 tcggcttgta tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc
    19801 tgctgccggg cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca
    19861 aggattgcca gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg
    19921 aaaaaatcat gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca
    19981 ttaccgccaa cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct
    20041 ggttaactta ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg
    20101 tactgaacca gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagccagg
    20161 aagagtctta ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg
    20221 ccgcatctgg catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta
    20281 catatttttc atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc
    20341 ggtggcgttt ccagcagttc cagcatagta cgggcaattt cacgttcacc catcactacc
    20401 tgattcgcgc cacgttcggt gatatacgtc acttcatcgt cataatgggc gcgggcgata
    20461 atctcaatat ccgggttttt cgcgcgggca gacgccacaa tctcacccgc ttcataaccg
    20521 ttggggatcg tcaggatcag ccattttgca cattccagat gtgccagttg cataatttct
    20581 tcgttcgctg cattgcccaa tacagcgcgg accccacgct ctcgcagctc atcaacacgg
    20641 gttcgtgacg tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc
    20701 aggctgccta cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc
    20761 tgtttctctt cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc
    20821 agatatttct ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct
    20881 gccagtacca ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc
    20941 aggataaacg cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt
    21001 tgggagtgac caaacagacg gacaaggaac agggccgcga ccgatttacc aaacagaata
    21061 attgccagcg tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac
    21121 atcccgactg agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcaa cgtatcgtgg
    21181 gcggcacggt gactcaattc agactcgttc agtaccatcc cggcaaagaa cgcaccgagt
    21241 gcaaaggaga catcaaacag ctctaccgca ccaaaggcaa tccctaacgc cagcgccagc
    21301 actgacaggg taaacagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc
    21361 accagacggc gacctaccag catcataatg gcgataaatg cgatcacctt gccgatggtg
    21421 atccccatat cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc
    21481 actgcgggca gcaacaccag cgtcagaacc atcaccaggt cttccacaat caaccaaccg
    21541 atggcgattt gcccacgctg actgtctatt aattgccgtt cttcaagtgc gcgcagtaac
    21601 accacggtac tggcggtgga aagacataag ccgaacacga taccggtcat taacgaccag
    21661 cccagcacgg cggagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgcg
    21721 ccgggaatgg cgatggactt taccgccatc aaatccttga gcgaaaaatg caaaccgacg
    21781 ccgaacatca gcaaaataac gcccagttca gccagttccg gcgcaagctt ggtatcggca
    21841 acaaagcccg gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga
    21901 gaaatacgta gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca
    21961 atggtggtga taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt
    22021 ccatttttgg ctgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga
    22081 tgtttatgaa taattgttga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg
    22141 ttcggaaaac agaaggcggt ttaacagagg aaaggcttat ggcgactgta aaacgatgca
    22201 gccaaagttt gcctttggct gcaatgaaat cagtctttat gccggttatc aggcaggaat
    22261 atggtcaaca tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatgctg
    22321 gtgtgatcgg cgataagccc cagaactgcc gctcccagac ctcccatgcc gaaagcaaaa
    22381 ccgaaaaaga gtccagaaac cataccgata cgtcccggaa gtagctcctg agcgtagacc
    22441 agaatggcag agaatgccga agcgaggata aatccaataa tcaccgttaa aacccccgtc
    22501 cagtgcaggc gggcgtaggg taaaatcagc gtaaacggcg caacgccgag gatagagccc
    22561 caaatcacat atttccgtcc aattttatcc cctacaggcc cgccgatcac cgtacctgcc
    22621 gcaacggcaa acaggaaggc aaacagatga agctgggcat tctggataga taatccgaat
    22681 ttttgcatca gataaaaggt gtaatagctg ctgatgctcg ccatatagaa atatttcgag
    22741 aaaatgagga ttaacagaat gctgactgcc agtaccactt tgttgcgcgg cagtggattg
    22801 attatcgtcg ctttgggttt tcctttattc attcggtgct gtgccgagta ccaacggcta
    22861 atttgcgcca acaccacgat cgccagcagt gccgcaagca caaaccaggc aacgttgcct
    22921 ttgccataag gcgcgataat caccgccgcc agcaagggtc ccagggaact gccaaagttg
    22981 ccgccgacct gaaagataga ttgcgccagg ccatgccgcc cgccggaagc catacgggct
    23041 acgcgagaag attccggatg aaagaccgat gaaccggtac cgaccagcgc cgctgccagc
    23101 agaactgcgc caaaactgcc cgccagcgca agcagcacca gaccacttaa ggtaaagcac
    23161 atgccaattg gcaacgacca cggcatcgga tatttatcgg tccagtagcc gaccactggt
    23221 tgcagtagcg aagaggcgag ctggaaggtg agggttatca tgccaatctg cataaatgtc
    23281 agagaaaatt ctgactgaag cagcggataa atcgccagaa tcagcgattg gatcatgtcg
    23341 ttcagcagat gtgagaggct gatagcacct aaaataccaa acgatgttcg ggccttggtc
    23401 gttgacgcag ccgcgcccgc cacaggctgg gtttgttcac tcattgccat aggaaagtca
    23461 ctttttcagg gttgcgatgt aaagaatgat cttatttgtg attattaccc gactaacata
    23521 cctgtatgcg tcgtctgaag gaagtctcaa cgccgaatac agaatttcta atctggatgc
    23581 agatttatct tcaccggacg cagacttgtc tatgatgttg cgtcatacta tttttcaaca
    23641 cgttgaaatc aggtcaggga gagaagtatg aaattattgc agcggggcgt ggcgttagcg
    23701 ctgttaacca catttacact ggcgagtgaa actgctctgg cgtatgagca ggataaaacc
    23761 tacaaaatta cagttctgca taccaatgat catcatgggc atttttggcg caatgaatat
    23821 ggcgaatatg gtctggcggc gcaaaaaacg ctggtggatg gtatccgcaa agaggttgcg
    23881 gctgaaggcg gtagcgtgct gctactttcc ggtggcgaca ttaacactgg cgtgcccgag
    23941 tctgacttac aggatgccga acctgatttt cgcggtatga atctggtggg ctatgacgcg
    24001 atggcgatcg gtaatcatga atttgataat ccgctcaccg tattacgcca gcaggaaaag
    24061 tgggccaagt tcccgttgct ttccgcgaat atctaccaga aaagtactgg cgagcgcctg
    24121 tttaaaccgt gggcgctgtt taagcgtcag gatctgaaaa ttgccgttat tgggctgaca
    24181 accgatgaca cagcaaaaat tggtaacctg gaatacttca ctgatatcga atttcgtaag
    24241 cccgccgatg aagcgaagct ggtgattcag gagctgcaac agacagaaaa gccagacatt
    24301 attatcgcgg cgacccatat ggggcattac gataatggtg agcacggctc taacgcaccg
    24361 ggcgatgtgg agatggcacg cgcgctgcct gccggatcgc tggcgatgat cgtcggtggt
    24421 cactcgcaag atccggtctg catggcggca gaaaacaaaa aacaggtcga ttacgtgccg
    24481 ggtacgccat gcaaaccaga tcaacaaaac ggcatctgga ttgtgcaggc gcatgagtgg
    24541 ggcaaatacg tgggacgggc tgattttgag tttcgtaatg gcgaaatgaa aatggttaac
    24601 taccagctga ttccggtgaa cctgaagaag aaagtgacct gggaagacgg gaaaagcgag
    24661 cgcgtgcttt acactcctga aatcgctgaa aaccagcaaa tgatctcgct gttatcaccg
    24721 ttccagaaca aaggcaaagc gcagctggaa gtgaaaatag gcaaaaccaa tggtcgtctg
    24781 gaaggcgatc gtgacaaagt gcgttttgta cagaccaata tggggcggtt gattctggca
    24841 gcccaaatgg atcgcactgg tgccgacttt gcggtgatga gcggaggcgg aattcgtgat
    24901 tctatcgaag caggcgatat cagctataaa aacgtgctga aagtgcagcc attcggcaat
    24961 gtggtggtgt atgccgacat gaccggtaaa gaggtgattg attacctgac cgccgtcgcg
    25021 cagatgaagc cagattcagg tgcctacccg caatttgcca acgttagctt tgtggcgaaa
    25081 gacggcaaac tgaacgacct taaaatcaaa ggcgaaccgg tcgatccggc gaaaacttac
    25141 cgtatggcga cattaaactt caatgccacc ggcggtgatg gatatccgcg ccttgataac
    25201 aaaccgggct atgtgaatac cggctttatt gatgccgaag tgctgaaagc gtatatccag
    25261 aaaagctcgc cgctggatgt gagtgtttat gaaccgaaag gtgaggtgag ctggcagtaa
    25321 tccgaaagtg ccggatgttt gcatccggca caatgcttaa tcgcggcggg cgatatcagc
    25381 aaatttggca tcgaggatct ttgccagatc gcctgccgcc agttcgatat ccagtccgcg
    25441 cttgccgccg gaaacataaa tagtggcaaa ttcttgtgcg ggggcgtcga taatcgttgg
    25501 cagacgtttt ttctgcccca gtgggctaat ccccccaacc aggtatcccg tcgaacgctg
    25561 cgcgaccatc ggatcggcca tctcaacttt cttggcaccc agcgcttttg ctactttttt
    25621 aagatccagt tgacctgcga ccggcgtaac ggccacggca aggtgtttca tatcaccgtt
    25681 cactgccacc agcagcgttt tgtagacctg atccggattc aaacctaatt ttttgacgac
    25741 ttcatcgcca aaattggttt cagccggatc gtgctcgtag gtatggattt gaaacgaaat
    25801 cttgtttttt tcgagtaatt taactgcggg tgtcatggta attttcctgc cttaaacaaa
    25861 aaaacgaagc aagcatacgc ctgatggcgg tccaaaaaaa agagtcatct tgcctaagag
    25921 tattggcagg atggtgagat tgagcgacaa tcgagttaca ccgtcgctta aagtgacggc
    25981 ataataataa aaaaatgaaa ttcctctttg acgggccaat agcgatattg gccatttttt
    26041 tagcgcaaca tttgcggcaa attcccttct ccatacaggt gtagtgcacc gaccgcgacc
    26101 acatatcgcc ccggcggcat ggcgcgtaat ttatcccgcc aggcgagatt tcgctgatgc
    26161 atcagcacat cgtacagcga ctgactgaac gtattgggca gcgttatatc attattttgc
    26221 ggcggtgcat tcagccacca gctcatcatt tgttgcagca accgtgcgtt ggtatgccag
    26281 tgggtcagcg tatcgtccag cagcgccagt cctttgtcag ggagctggag caacatggca
    26341 atctggtttt cagccccttc cagttcaatc acgggtttat gttgttgctt cgccgcctgc
    26401 aatagctggt aatcgatacc gtattctgcc cgcagcccca gtttttgtgc ctgcgtcgcc
    26461 tgaagaacca tcgcgatttg ccacagcggt tgggtagaaa agagtgaggg agaaatgccc
    26521 atctcctggc taatgtgctg caggttttgg agttgctcct cgctaatgcg ctcttccagc
    26581 gcctcgcagg caggcaaatt agcaaaaggc gtatcgctgg tggaaacatc cgcctcgacg
    26641 atcagcgcat cggcgttttt gagctttttg agcaaacggg tgggcagggg agccatatcg
    26701 tggctaccca tatgaatact gccaatcaga tgaaaatggc gattgccggg gagggtgata
    26761 tcgatggcag gccaggtgta atgattaccg cgcagcgcgg cccaaagcgt ttttacccgg
    26821 tacaacagat ccatacgacc tccctttgtg aaatatcatg ctagcgcgcg gtgagggatg
    26881 gcgcaaatgg agttggggac ttgtttaatg tgtttgtatg attcagtatg ttcttgcatc
    26941 gctattcaca gggaagcaac agttaaaaac catgaaacag gcaacaagaa aaccgacgac
    27001 acccggagat attcttctct atgaatatct ggaaccgctc gatttgaaaa tcaatgagtt
    27061 agcagagttg ctgcatgttc atcgtaatcg cgtcagtgca ctgatcaata acaatcgtaa
    27121 actcaccact gagatggcat tccgtctggc gaaagttttt gataccacag tcgattttag
    27181 gctaaacctc caggcggcag ttgatctttg ggaagttgaa aacaacatgc gcacccagga
    27241 agaattggga cggattgaaa cagtggctga atatttggca cgccgtgaag agcgtgcaaa
    27301 aaaggtcgcg taaggccaaa acatcgcttc atctcgtagg ccggataagg cgttcacgcc
    27361 gcatccggca tccgagcacc attgcctgat gcgacgctta cgcgtcttat caggcctaca
    27421 aacctgtgcc tgaaccgtag gccggataag gcgtttacgc cgcatccggc agtcatgcgt
    27481 cgatgccaaa tgcgccgccc taaagcagcg catccgcaat gacgtactta ttccttcggt
    27541 ttaaaccgca gcaaccggtt ggcgttactt actacggtaa ttgacgagag cgccattgcc
    27601 gctccggcaa ctaccgggtt aagcagtgtt ccagtaaacg gccacaaaat accggcggcg
    27661 accggaatac cgatgctgtt gtagataaag gcaccgagca ggttctgctt catgttgcgc
    27721 agcgttgcgc gggaaatcgc gagcgcatcc gcaacgccca tcaggctatg gcgcatcagg
    27781 gtaatcgccg cggtttcaat ggcaacatca ctgccgccac ccatcgcaat gccgacatcc
    27841 gcctgggcca gcgctggcgc gtcgttaatg ccgtcgccca ccattgccac ctgacgtcct
    27901 tcactttgca gacgtttgat cgcttcggct ttaccgtccg gcagcactcc ggcaatcact
    27961 tcatcaattc cagcttcttt ggcgatcgca ttggcggtgg ttgggttatc cccggtcaac
    28021 atcaccagac gatatcccgc tttatgcagg cgttgcagcg ccgccacgct atcactacgc
    28081 aacggatcgc gtacagccag cagggctact gctttcccgt caatcgccag cagcacaggc
    28141 gttgctcctt gcgatgcctg agctgaaata tcggcttcaa tcgctttagt atcgacttgc
    28201 tgatcattta acaacgcctg attacccagc aataacgcat gaccttctgc ttcaccgctc
    28261 acgcccagcc cgcgcaatgt gcggaaaccg ttgacctgcg gtagctgcat atcgcctgct
    28321 ttatcaagga tcgctcgtgc caatggatgg ctggaacctt gctccagcgc cgccgccaga
    28381 cgcagcgcct gcgcttcgtc aacatcagca aatgttttca ctgcgacaac ctgcggcttc
    28441 ccttcggtca gcgtcccggt tttatcgaac acgacggtat cgagtgtact ggcgcgttgc
    28501 agcgcgtcgg cgtcccgcac cagcacgcca aactcagccg cccgcccgac gccggaaata
    28561 atcgacatcg gcgtcgccag tcccagcgca cacgggcagg cgataatcag caccgtagtg
    28621 gcaatcacca gggtatagac aatctgcggt gccggaccaa agaaatacca gattgccgca
    28681 ctgacaagcg caataaccac tactaccggc acaaatatgg ctgagatttt atccgccagc
    28741 tgaccgattt ctggcttgct gctctgggcc tggcgcacca tgcgaatgat ccttgatagc
    28801 gtggtatggc tgccaaccgc actggcacga aacagcacac tgccgtcctg taccactgtc
    28861 ccggcatgga cgctatcgcc ttcgcctttt tgctgcggga ttggttcgcc cgtcagcatc
    28921 gcttcatcca gccatgcttc gccctgggta atctcgccat ctaccggcac gcgatcgccg
    28981 gtcgtcaggc gcagcaacat acctggctgc acttctgcca gaggcacgct tttttcacct
    29041 tcgtcagtaa ccaggcgcgc cgtcggtggg gttaaatcga gtaatttctc cagtgcctta
    29101 gaagaacgct gacgtgcgcg tgcttccagc atatggccga gattgatcag accgataatc
    29161 atcgcactgg cttcatagta aagatgtcgc gcttccatcg ggaaccactg cggccacagg
    29221 ttgacgctca tcgaatagag ccacgccacg cccgttccca gcgccaccag cgtatccatc
    29281 gtcgctgcac cgttcagcag gcttttccat gcactgcggt aaaaatggcc gctggcgaaa
    29341 accatcaccg ccagggttat caggccgata accaaccaca ggctgcggtt gtcggcggtg
    29401 actatcatgt tatcgccgat catcccccag accatcaccg ggatacccac cgccagtgcg
    29461 acaattgcct gccagcggaa gcgcttcatc gtagcgacgg cggtttcttg ctggcgttcg
    29521 cggcgtttcg cgtcatcttc aatcgcttcc gcgccgtagc ccgctttttc caccgcctgc
    29581 actaaatctt gtggggaggc actgcccatc accagcgcag tacgctccgc caggtttacc
    29641 cgtgcctgag tgacgcccgg tacgctttgc agcgcatttt gtacgcgggt gacacagctg
    29701 gcgcagctca tgccgctcag cagcaactgc tggctgtcat cgtcatcggc ggtcgctgcc
    29761 ggaagcgcct cagaaaccgc tgtcagtgct tccgacggga ttgatgactc cgccagcggt
    29821 ttagcctttg ggtggcttac agatgcgtca taacccgctt gtttgatggt ttcgatcagc
    29881 tgttctgcac tggcagtccc ggtaacgtgc gcttcagtga tagacacatc cgcttgttcc
    29941 acatccgggc gctgttcaag actttctttc acgcgtttaa cgcagtgacc gcaggacagg
    30001 ccgtccaggt tcaggtcgat agtttgtgac ataaaacact cctttaagac agttttgact
    30061 ggctgtgata aaggttaaac cttccagcaa ggggaaggtc aagaaattaa taaaccaggc
    30121 gggtaaaagt ccgtaaagat taaaaaatcg gctcgatttg catcaggatt agacatttat
    30181 ttctttgttt tcctgtagtt aagttgcggg tgctaagtta aatcaggatg cctgaaaatc
    30241 ggcactgggg tgaggaatta cctcccgcat ctataaaaag gagttaacaa aagatgttag
    30301 atgcaaacaa attacagcag gcagtggatc aggcttacac ccaatttcac tcacttaacg
    30361 gcggacaaaa tgccgattac attccctttc tggcgaatgt accaggtcaa ctggcggcag
    30421 tggctatcgt gacctgcgat ggcaacgtct atagtgcggg tgacagtgat taccgctttg
    30481 cactggaatc catctctaaa gtctgtacgt tagcccttgc gttagaagat gtcggcccgc
    30541 aggcggtaca ggacaaaatt ggcgctgacc cgaccggatt gccctttaac tcagttatcg
    30601 ccttagagtt gcatggcggc aaaccgcttt cgccactggt aaatgctggc gctattgcca
    30661 ccaccagcct gattaacgct gaaaacgccg aacaacgctg gcagcgaatt ttacatatcc
    30721 aacagcaact ggctggtgag caggtagcgc tctctgacga agtcaaccag tcggaacaaa
    30781 caaccaactt ccataatcgg gccattgcct ggctgctgta ctccgccgga tatctctatt
    30841 gtgatgcaat ggaagcctgt gacgtgtaca cccgtcagtg ctctacgctc atcaatactg
    30901 ttgaactggc aacgcttggc gcgacgctgg cggcgggtgg tgtgaatccg ttgacgcata
    30961 aacgcgttct tcaggccgac aacgtgccgt acattctggc cgaaatgatg atggaagggc
    31021 tgtatggtcg ctccggtgac tgggcgtatc gcgttggttt accgggcaaa agcggtgtag
    31081 gtggcggtat tctggcggtc gtccctggcg tgatgggaat tgccgcgttc tcaccaccgc
    31141 tggacgaaga aggcaatagt gttcgcggtc aaaaaatggt ggcatcggtc gctaagcaac
    31201 tcggctataa cgtgtttaag ggctgatcat gatgaacacg gaaggtaata acggtaacaa
    31261 acccctcggt ctatggaacg tcgtttccat cggtattggg gcaatggtgg gggcggggat
    31321 cttcgcgctg ctggggcagg ctgcgttgct aatggaagcc tcgacctggg gcgcctttgc
    31381 ttttggcggt attgtggcta tgttttccgg ttatgcctat gcgcgtctgg gggcgagcta
    31441 tcccagcaat ggcggtatta tcgacttttt tcgtcgcgga ttaggcaacg gcgtcttttc
    31501 gctagcgctc tcgttgctat acctgttgac gctggcggtg agcatcgcca tggttgcccg
    31561 tgcttttggc gcttatgccg tgcagttttt gcatgaaggc agccaggagg agcaccttat
    31621 tttgctctac gcgttgggga tcattgcggt gatgacgctt ttcaactcct taagcaacca
    31681 tgcggtaggg cggctggaag tgatcctcgt cggcattaaa atgatgatcc tgttattgct
    31741 gattattgcc ggtgtctggt cgctgcaacc ggcgcatatt tccgtctctg cgccccccag
    31801 ctccggtgcg ttcttctcct gtattgggat aaccttcctt gcctatgcgg gctttggcat
    31861 gatggcgaac gcggcggata aagtgaaaga tccgcaggtc attatgccac gggcgtttct
    31921 ggtggcgatt ggcgttacca cgttgcttta tatctcgctg gcgctggttt tgcttagcga
    31981 tgtatcggca ttagagttag aaaaatatgc tgataccgcc gtagcgcagg ctgcttctcc
    32041 gctgctcggg catgtgggtt atgtgatcgt cgtcatcggc gctttactgg cgacggcttc
    32101 aggcattaac gcgaacctgt tcgccgtgtt taacatcatg gacaacatgg gcagcgaacg
    32161 cgaactgccg aagctaatga ataaatccct gtggcggcag agtacctggg gcaatatcat
    32221 cgtcgtggtg ttgataatgc tgatgacggc ggcgctaaat ttagactcac tcgccagcgt
    32281 tgccagcgcc acctttttga tctgctatct ggcggtgttt gtggtggcga tccgcctgcg
    32341 tcatgatatt cacgcctcgt tgccgattct tatcgttggt acgttggtga tgttgttggt
    32401 gatcgttggc tttatctaca gcctgtggtc ccagggcagc cgtgcgttga tatggattat
    32461 tggtgcaatc ttactcagcc ttattgtggc aatggtcatg aagcgtaata aaaccgtata
    32521 acacctctct gtgcgcagta cttcctgtat tattgtggtg gcggtcgata ttcgcactgg
    32581 caaaaaaacg tgcttgaata tctgttgaaa ccctttaaca aagcacagga ggcgttgcgc
    32641 gaacgatgaa catcagcgat gtagcaaaaa ttaccggcct gaccagcaaa gccattcgct
    32701 tctatgaaga gaaggggctg gtgacgccgc cgatgcgcag cgaaaacggt tatcgcacct
    32761 acacgcagca gcatctcaac gaactgacct tactgcgcca ggcacggcag gtgggcttta
    32821 acctggaaga gagcggcgag ctggtgaatc tgtttaacga cccgcagcgg cacagcgccg
    32881 acgtcaaacg gcgcacgctg gagaaggtgg cggagatcga acgacacatt gaggagctgc
    32941 aatccatgcg cgaccagctg ctggcactgg cgaatgcctg ccctggcgat gacagcgccg
    33001 actgcccgat tatcgaaaat ctctccggct gctgtcatca tcgggcaggg tgattaagac
    33061 gagacggctc ggatatgtag ggttatccct tctatcgcaa tgacttcaac atgcgtacct
    33121 gcaccgagat cctcgctggc gctgacaggc catgaactgt caccgacgcg catatgaccg
    33181 cgcccgttga ccagcggaga ttccagcaca aaacgtcggc caatcagctg ctgcccgcgc
    33241 tggtttaaat gactatcgct gtgcttttgt tcgcgcaccc gccgcgacaa ccatttccac
    33301 cacagccagg cggcgagcag cgtcaggatg gcaaacatta ccccttgcca ctcccaaccc
    33361 agcggcacca gccagaccac caggccggta atcaccgctg ccacgccgct ccacaacaaa
    33421 taaccatttc cgcccagcat ctcggctgcc agcagcaacc cgccgagact gagccagaaa
    33481 atatgtggat gaacgaccat taactccatc atggctgagt ccgtttgttg gcgctgtctt
    33541 tcaccagctc ggcaatcccg gcaatcgacc ccatcaggct gctggcctct aatggcatca
    33601 tcactacttt gctgttactg gaggaaccga tctgctgtaa cgcttcggtg tatttttgcg
    33661 ctacgaagta gttcaccgcc tgaatatcac cggaggcgat agcttcagac accattttgg
    33721 tggcgcgggc ttctgcttcg gcggaacgtt cacgcgcttc agcctgtaaa aacgccgact
    33781 gacgttcgcc ttccgctttc aggatttgcg actgtttttc accttcggct ttgaggattt
    33841 ccgcctgacg gatcccttcc gcttcaagaa tgtaagcgcg tttggtacgt tccgctttca
    33901 tctgcgcgtt cattgaagag ataagctctg ccggtgggcg cacgtcgcga atttcaatac
    33961 gggtgacttt aatcccccac gggttggtgg cttcatcgac aatacgcagc aggcgtgagt
    34021 tgatgctgtc gcgctgagag agcatttcgt caagttccat tgaacccaac acggtacgga
    34081 tgttagtcat ggtcaggttg atgatcgcca gctccagatt gctgacttca taggcagcgc
    34141 gtggcgcgtc aatcacctga ataaaacaga ctgcgtcgat ggtaacgttg gcgttatctt
    34201 tcgagataac ttcctgggaa gggatatcga gcacttgctc catcatattg atcttgcgac
    34261 caatgcgatc cataaacggc accaccagac tgagccccgg ctgtaacgtt ttggtatagc
    34321 gaccaaaacg ttctaccgtc cattgatagc cctgcggcac gattttgacg cccgcgccga
    34381 caatgaccag cgcgacaaaa atgagaatcg ggataaagat aagcatcgga aaaacctcct
    34441 gttgtaccgt ccataatcag caaaattgct gcttgattaa acaaattata cctgattact
    34501 gaaagagagt tcccccttat tcctgcgaag gataaactgt ttttagtaaa aatcagaaaa
    34561 agggaacagc gatgcaggaa aatagtcctt tgcttcagct acaaaacgta ggatatctgg
    34621 cgggtgatac gaagattctt aataacatca atttttcgct gcgtgctggc gaatttaagt
    34681 taattaccgg tccttctggt tgtggcaaaa gtacgctgct aaaaatagtt gcttcattga
    34741 tcagcccaac cagcggaacg ttactgtttg aaggtgagga tgtcagcaca ctaaaaccag
    34801 aaatctaccg ccagcaagtc tcttactgcg cccagacgcc gacgctgttt ggcgatacgg
    34861 tatacgataa tctgatcttt ccctggcaga tccgtgacca gcagcctgac ccggccattt
    34921 ttctcgattt tctcgaacgc ttcgctttgc cggacagcat tttgacgaag aatatcgccg
    34981 agctatctgg tggtgaaaaa caacgcatct cattgattcg taacctgcaa tttatgccga
    35041 aggttttatt gctggatgaa ataaccagtg cgctggatga aagtaataaa cataacgtca
    35101 atgagatgat ccatcgttat gtgcgcgagc aaaatattgc cgtgctgtgg gtgacacacg
    35161 ataaagacga aattaatcat gcggataaag tgattacact gcaaccgcat gccggagaaa
    35221 tgcaggaagc acgctatgaa ctcgcataat attactaacg aatcattagc actggcatta
    35281 atgctggtgg tggtggcaat cttaattagc cataaagaaa aactggcgct ggagaaagat
    35341 attctatgga gcgtcgggcg agcgataatt cagctgatta ttgtcggcta tgtgctgaag
    35401 tatattttca gcgtggatga tgccagcctg acattattga tggtgttatt tatctgcttt
    35461 aatgcggcgt ggaacgcgca aaa
//
LOCUS       DAZXUK010000013        35476 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZXUK010000013 DAZXUK010000000
VERSION     DAZXUK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35476
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.013"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..610)
                     /gene="mltG"
                     /locus_tag="ACH3L8_000542"
     CDS             complement(<1..610)
                     /gene="mltG"
                     /locus_tag="ACH3L8_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFT4083797.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYK"
     gene            complement(613..1422)
                     /gene="pabC"
                     /locus_tag="ACH3L8_000543"
     CDS             complement(613..1422)
                     /gene="pabC"
                     /locus_tag="ACH3L8_000543"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFT4083798.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(1542..2783)
                     /gene="fabF"
                     /locus_tag="ACH3L8_000544"
     CDS             complement(1542..2783)
                     /gene="fabF"
                     /locus_tag="ACH3L8_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFT4083799.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(2870..3106)
                     /gene="acpP"
                     /locus_tag="ACH3L8_000545"
     CDS             complement(2870..3106)
                     /gene="acpP"
                     /locus_tag="ACH3L8_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFT4083800.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(3317..4051)
                     /gene="fabG"
                     /locus_tag="ACH3L8_000546"
     CDS             complement(3317..4051)
                     /gene="fabG"
                     /locus_tag="ACH3L8_000546"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFT4083801.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(4064..4993)
                     /gene="fabD"
                     /locus_tag="ACH3L8_000547"
     CDS             complement(4064..4993)
                     /gene="fabD"
                     /locus_tag="ACH3L8_000547"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFT4083802.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(5009..5962)
                     /gene="fabH"
                     /locus_tag="ACH3L8_000548"
     CDS             complement(5009..5962)
                     /gene="fabH"
                     /locus_tag="ACH3L8_000548"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFT4083803.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(6030..7100)
                     /gene="plsX"
                     /locus_tag="ACH3L8_000549"
     CDS             complement(6030..7100)
                     /gene="plsX"
                     /locus_tag="ACH3L8_000549"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFT4083804.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(7181..7354)
                     /gene="rpmF"
                     /locus_tag="ACH3L8_000550"
     CDS             complement(7181..7354)
                     /gene="rpmF"
                     /locus_tag="ACH3L8_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFT4083805.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(7406..7927)
                     /gene="yceD"
                     /locus_tag="ACH3L8_000551"
     CDS             complement(7406..7927)
                     /gene="yceD"
                     /locus_tag="ACH3L8_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFT4083806.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            8126..8710
                     /gene="yceF"
                     /locus_tag="ACH3L8_000552"
     CDS             8126..8710
                     /gene="yceF"
                     /locus_tag="ACH3L8_000552"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFT4083807.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(8822..9781)
                     /gene="rluC"
                     /locus_tag="ACH3L8_000553"
     CDS             complement(8822..9781)
                     /gene="rluC"
                     /locus_tag="ACH3L8_000553"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFT4083808.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            10354..13545
                     /gene="rne"
                     /locus_tag="ACH3L8_000554"
     CDS             10354..13545
                     /gene="rne"
                     /locus_tag="ACH3L8_000554"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFT4083809.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(13742..14684)
                     /gene="flgL"
                     /locus_tag="ACH3L8_000555"
                     /pseudo
     CDS             complement(13742..14684)
                     /gene="flgL"
                     /locus_tag="ACH3L8_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(14696..16338)
                     /gene="flgK"
                     /locus_tag="ACH3L8_000556"
                     /pseudo
     CDS             complement(14696..16338)
                     /gene="flgK"
                     /locus_tag="ACH3L8_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(16404..17345)
                     /gene="flgJ"
                     /locus_tag="ACH3L8_000557"
     CDS             complement(16404..17345)
                     /gene="flgJ"
                     /locus_tag="ACH3L8_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFT4083810.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(17345..18442)
                     /gene="flgI"
                     /locus_tag="ACH3L8_000558"
     CDS             complement(17345..18442)
                     /gene="flgI"
                     /locus_tag="ACH3L8_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFT4083811.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(18454..19152)
                     /gene="flgH"
                     /locus_tag="ACH3L8_000559"
     CDS             complement(18454..19152)
                     /gene="flgH"
                     /locus_tag="ACH3L8_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFT4083812.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(19205..19987)
                     /gene="flgG"
                     /locus_tag="ACH3L8_000560"
     CDS             complement(19205..19987)
                     /gene="flgG"
                     /locus_tag="ACH3L8_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFT4083813.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(20125..20712)
                     /gene="flgF"
                     /locus_tag="ACH3L8_000561"
                     /pseudo
     CDS             complement(20125..20712)
                     /gene="flgF"
                     /locus_tag="ACH3L8_000561"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(20814..20876)
                     /locus_tag="ACH3L8_000562"
                     /pseudo
     CDS             complement(20814..20876)
                     /locus_tag="ACH3L8_000562"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20896..22104)
                     /gene="flgE"
                     /locus_tag="ACH3L8_000563"
     CDS             complement(20896..22104)
                     /gene="flgE"
                     /locus_tag="ACH3L8_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFT4083814.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(22129..22824)
                     /gene="flgD"
                     /locus_tag="ACH3L8_000564"
     CDS             complement(22129..22824)
                     /gene="flgD"
                     /locus_tag="ACH3L8_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFT4083815.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(22836..23239)
                     /gene="flgC"
                     /locus_tag="ACH3L8_000565"
                     /pseudo
     CDS             complement(22836..23239)
                     /gene="flgC"
                     /locus_tag="ACH3L8_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(23243..23659)
                     /gene="flgB"
                     /locus_tag="ACH3L8_000566"
     CDS             complement(23243..23659)
                     /gene="flgB"
                     /locus_tag="ACH3L8_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFT4083816.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            23814..24473
                     /gene="flgA"
                     /locus_tag="ACH3L8_000567"
     CDS             23814..24473
                     /gene="flgA"
                     /locus_tag="ACH3L8_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFT4083817.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            24549..24842
                     /gene="flgM"
                     /locus_tag="ACH3L8_000568"
     CDS             24549..24842
                     /gene="flgM"
                     /locus_tag="ACH3L8_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFT4083818.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            24847..25263
                     /gene="flgN"
                     /locus_tag="ACH3L8_000569"
     CDS             24847..25263
                     /gene="flgN"
                     /locus_tag="ACH3L8_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFT4083819.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(25303..26838)
                     /gene="murJ"
                     /locus_tag="ACH3L8_000570"
     CDS             complement(25303..26838)
                     /gene="murJ"
                     /locus_tag="ACH3L8_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFT4083820.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(27005..27871)
                     /locus_tag="ACH3L8_000571"
                     /pseudo
     CDS             complement(27005..27871)
                     /locus_tag="ACH3L8_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(27873..28520)
                     /locus_tag="ACH3L8_000572"
     CDS             complement(27873..28520)
                     /locus_tag="ACH3L8_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HFT4083821.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(28531..29115)
                     /gene="rimJ"
                     /locus_tag="ACH3L8_000573"
     CDS             complement(28531..29115)
                     /gene="rimJ"
                     /locus_tag="ACH3L8_000573"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFT4083822.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            29351..30559
                     /gene="mdtH"
                     /locus_tag="ACH3L8_000574"
     CDS             29351..30559
                     /gene="mdtH"
                     /locus_tag="ACH3L8_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFT4083823.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            30623..31270
                     /gene="grxB"
                     /locus_tag="ACH3L8_000575"
     CDS             30623..31270
                     /gene="grxB"
                     /locus_tag="ACH3L8_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFT4083824.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            31404..31964
                     /locus_tag="ACH3L8_000576"
     CDS             31404..31964
                     /locus_tag="ACH3L8_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFT4083825.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            32070..33116
                     /gene="pyrC"
                     /locus_tag="ACH3L8_000577"
     CDS             32070..33116
                     /gene="pyrC"
                     /locus_tag="ACH3L8_000577"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFT4083826.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            33190..33435
                     /gene="dinI"
                     /locus_tag="ACH3L8_000578"
     CDS             33190..33435
                     /gene="dinI"
                     /locus_tag="ACH3L8_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFT4083827.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            33725..33979
                     /gene="bssS"
                     /locus_tag="ACH3L8_000579"
     CDS             33725..33979
                     /gene="bssS"
                     /locus_tag="ACH3L8_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFT4083828.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            34094..35212
                     /gene="solA"
                     /locus_tag="ACH3L8_000580"
     CDS             34094..35212
                     /gene="solA"
                     /locus_tag="ACH3L8_000580"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFT4083829.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            35260..35373
                     /locus_tag="ACH3L8_000581"
     CDS             35260..35373
                     /locus_tag="ACH3L8_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFT4083830.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT         8513 a         9582 c         8822 g         8559 t
ORIGIN      
        1 ctttataagg cagaccgtcc gcacgccctt cccaggcgct atcgaccgct ttcaccattt
       61 tcttgtgaac tcgcttgagt aacgcgacat cggtggtatt ggcggtatac atcaaggtgt
      121 ctggccagaa ccaaccttca atccactccg ggttttccag ttcaagtgcc tgcgctacgg
      181 tggcgtactt atcgtcactc agcgtgtgct tgatatacgg agcctcacgc aattgcttga
      241 ggtagtcgct cagacgcatc ccttctacca gtcgcagcgg gaactgtgct tctttaccgc
      301 tttccaacaa tttcagcatc tcgcgcacgg tcatctgcgg tgtaaagcgg taagtcccgg
      361 ctttaaagtg agaaagatcc ggttcgatac gcagcagcca ttgaaaaacc cgtgggcgat
      421 tgatgatctt atcagcataa agctgttcac cgagcgccag acgtccggtc cctggcttca
      481 gggtaaatat cgtctcttct ttgataagca atttgctgtc ggcaagatgg cgaaccttcc
      541 agacgcccac accagcggcg atacccagta ccaccagcaa taacaagata atcaataaca
      601 cttttttcat gactaattcg ggcgctcaca aagtggggct aaatattcat ataacgttgc
      661 tgacgaaaag gtgacatcgc cacaggcacg tacgggcatc actggcatta acgcattaca
      721 aataaccatc tcatctgcct gcaacgcctc ctccagcgag gcttgcactt cgacaagctg
      781 ataagaggat tgtgccagta aacggataca gaattgtcgc ataatgccgt taacacctgc
      841 ctgatccagt cgcggcgtat aaactacatt gcccttccgc cagaacaaat tagccgcaca
      901 gcattccgta acccaccctt cgctgtcaag gaccagcgcc tcatcagcgt ttgtctgctc
      961 aagatgagag cgaatcaata cttgctcaag ccgattaaga tgtttaatac ctgcaagatg
     1021 aggattgcgc cccagccgca ccgggcttag cgccaacgtc atcccttcgt tacgcaaacg
     1081 gtcgtaatgt gcaggataag ccgtaacgga gagaatccgc gttgctggtc cgctgttcaa
     1141 tgtgctgtac cctcgcccgc cactaccgcg actgatcacg actttcagca caccattttg
     1201 ctgttctgct gccagcgttt tcatctcttg ttcaagctga ggccagaagt cacaggaaat
     1261 catcaaccgc tgacaagcat cctgtagtcg caggatatgc gccgataaca aactgacttt
     1321 accgtcgata actctggcgg tggtaaaaca accatcacca aactgcgttg cccgatcgct
     1381 tactgccagc gattcctgct tataaccgtt aattaagaac ataccggctc cttatcagag
     1441 aattgttagt gtggcagcat gttcactacg gaacaagtcg gaataaaagc taagaaaaaa
     1501 ggcccgcaag cggacctttt atatgggtgg aaaatgacaa cttagatctt tttaaagatc
     1561 aaagaaccat tagtgccacc gaagccgaag gagttacaca gcgtgtattc cattccgctg
     1621 acctgacgtg cttcatgcgg tacgaaatcc agatcgcaac cttcatccgg gttatccagg
     1681 ttgatggttg gcggaacagc ctgatcgcgc agcgccagga tggagtagat agattctact
     1741 gcacccgccg cacctaacag gtgaccggtc atagatttcg tggagcttac caacacacgg
     1801 cttgcagctt caccgaagat ggttttcacc gcctgcgctt cagctttatc gccagccggc
     1861 gtagaagtac cgtgcgcgtt aacgtagcca atctgactcg cttcaatgcc tgcatcacgc
     1921 agagcatttg ccattgccag agctgcgcct gcgccatttt ctggcggtga cgtcatatga
     1981 taagcatcgc tgctcatacc aaagccgacg agttcagcgt aaattttcgc accgcgtttt
     2041 ttcgcgtgtt cgtactcttc aagtaccagc ataccggcac catcgcccag tacgaagcca
     2101 tcacgctctt tatcccacgg gcggctcgcc gcttgcgggt tatcattgcg ggtagataat
     2161 gcacgtgccg cgccaaaacc accaacgccc agcggcgtac tggctttctc tgcgccacct
     2221 gcaaccatca cgtcagcatc gccatacgcg ataatacgcg cagcatggcc aatgttgtgc
     2281 acgccggaag tacaggcggt cgcgatagag atgcttgggc cacgcaggcc atacatgata
     2341 gtcagatgac ctgccaccat gttcacaatc gttgacggaa cgaagaatgg gctgatctta
     2401 cgtggaccac cgttcatcag agatgtgtgg ttttcttcga tcagtccgag gccgccaatc
     2461 ccggagccaa ttgcggcacc aatgcgggtt gcgttctctt ccgttatttc aaggccagaa
     2521 tcctgcatgg cctgaacgcc agcgacaatt ccatattgaa tgaaggcatc catcttgcgc
     2581 tgttctttgc gcgagataat gtcctcacag ttaaaatcct ttactaagcc agcaaatttc
     2641 gttgcatagg cgctagtatc gaaatggtcg attaggctga tgccactctg accggcaagc
     2701 agagctttcc aggtagactc tacggtattg ccgacaggag acaacatgcc cagtccggtc
     2761 acaactacac gacgcttaga cacgtttgtc ctccaaggag ggaaaaatga ttctagtggg
     2821 acaaaaagat aaaactcagg cggtcgaacg accgcctgga gatgttcact tacgcctggt
     2881 ggccgttgat gtaatcaatg gcagcctgaa cggtggtgat tttctcagct tcttcgtccg
     2941 gaatctcagt atcaaactct tcttccagag ccattaccag ctcaacggtg tcaagagaat
     3001 ccgcgcccag gtcttcaacg aaagaagcat tgttggtaac ttcttcctgc ttaacgccca
     3061 gctgttcgcc gataattttc ttaacgcgtt cttcgatagt gctcatactc ttaaatttcc
     3121 tatcaaaact cgctttcgcg atggttttcg tagtgtataa aatgttgaaa aatttgcaac
     3181 taaatcccgg caggtcttac cacgatttta cgttattttg aggccttacc ccccaataac
     3241 gcaaatcatt ttgcactaat tattactcat aaccacgcag actgcgcgca acatgagcat
     3301 tttgtgcaaa tcgcggtcag accatgtaca tcccgccgtt cacatgcaaa gtttcacccg
     3361 tgatgtaagc tgcttcgtcg gatgccagga atgcaaccgc gttggcgatt tcctgtgcgc
     3421 cgccgaggcg acccgcagga acctgcgcca ggatacccgc acgctggtca tcgctcagcg
     3481 cacgtgtcat gtccgtttca ataaagcccg gagcaacaac gtttacagta ataccgcgtg
     3541 acgcaacttc gcgcgccagt gatttactga agccgatcaa gcccgctttc gccgcagcgt
     3601 agttggcctg accgccattt cccatggtac caaccacaga accgatagtg ataatacgac
     3661 catgacgctt tttcatcata gcgcgcatta ccgcttttga cagacggaaa acagatgaaa
     3721 ggttagtttc gataatatcg ttccactctt catctttcat tcgcattaac aggttatcac
     3781 gagtgatacc ggcattattg accaggatat ccacttcacc aaattctgcg cgaatttttt
     3841 ccagaacaga ttcgatagat gccgggtcgg tcacattcaa catcagacct ttgccgttgg
     3901 cacctaaata atcactgatc gcctgagcgc cattttcact ggtcgcagtg ccaataactt
     3961 tcgcgccacg ggctgcgagc gtttcagcaa ttgcgcggcc aattccgcgg cttgcaccgg
     4021 ttaccagtgc gatttttcct tcaaaattca tgattttcct cttttaaagc tcgagcgccg
     4081 ctgccatcgc tgaaggttcg ttcagcgccg aggcggtcag ggtgtcgaca atgcgtttcg
     4141 tcaggccagt aagcaccttg cccgggccga cttcatagag atgttctacg ccttgcgctg
     4201 ccatgtactc aacagacttc gtccactgaa ccgggttata caactgacgt accagtgcgt
     4261 cacggatggc atcaccattg gtttcgcatt tcacatcaac gttattcaca acagaaactg
     4321 ttggtgcgtt aaaggtgatt ttcgctaatt ctactgccag tttgtcggct gctggtttca
     4381 tcagcgcaca gtgagacggt acgctcactg gtaacggcag cgcgcgtttt gcgccagcag
     4441 ctttacaggc agcgccagca cgctcaaccg cttctttatg accggcaata accacctgtc
     4501 ccggagagtt aaagtttacc ggagaaacga cctgaccttc tgcagcttct tcacacgctt
     4561 tcgcaatagg cgcatcatcc agaccgatga ttgccgccat agcgcccgtg ccttccggta
     4621 cggcttcttg catgaactta ccgcgcatct caaccagacg caccgcatca gcgaaatcaa
     4681 tcacgccagc gcaaaccagc gcggagtatt cccccaggct gtgaccggcc atcattgccg
     4741 gtgctttacc gccctgctgc tgccatacgc gatacagcgc aacagatgca gtcaacagcg
     4801 ctggctgggt ttgccaggtt ttattcagtt cttcagctga cccctgctgg gtcagcgccc
     4861 acaggtcgta gcccagcgcc gcagaagctt cagcaaacgt ttcttcgaca attggatagc
     4921 tcgccgccat atcagccagc attccaacgg tttgagaacc ctgtccaggg aacacaaatg
     4981 caaattgcgt catgttttaa tccttatcct agaaacgaac cagcgcagag ccccaagtga
     5041 atccaccgcc aaaggcttca agcagaacca actgccccgg cttaatgcgc ccgtcgcgta
     5101 cagcttcatc cagcgcgcac gggacagagg ccgcagaggt attaccgtgg cgatccagcg
     5161 tcaccacgac attgtccatc gacataccga gttttttcgc cgttgcactg ataatacgca
     5221 ggttagcctg atgcggaacc agccagtcca gttgagaacg gtcaagatta ttcgccgcca
     5281 gcgtctcatc aacgatgtgc gccagttccg ttaccgcaac cttgaagact tcgttgcccg
     5341 ccatcgtcag atgaattgaa ttctctggat tcacgcggtc ggcgtttggc agcgtcagca
     5401 attcaccata actaccgtcg gcatgcagat gggtggaaat gattcccggc tcttcagagg
     5461 cagccagcac cgcagcgccc gcgccatcgc caaaaataat aatagtccca cgatctgttg
     5521 gatcgcaggt gcgcgccagt acatcggaac cgacgaccag agcatacttc accgccccag
     5581 atttcacgta ttgatcggct acgcttaatg cataggtgaa acctgcacag gctgctgcaa
     5641 cgtcaaatgc cgggcaacct ttaatgtcca acatgctttg aatctgacaa gctgcgctcg
     5701 ggaaagcgtg cgtagcagaa gtcgttgcca caacgatcag gccaatctgg tctttctcaa
     5761 tgcccgccat ctcaattgcg cgtgtcgccg cttcaaagcc catggttgaa acggtttcgt
     5821 ttggcgcggc aatgtggcgt tcgcggatac cggtacgagt gacaatccac tcgtcagagg
     5881 tgtccaccat tttttccaaa tcggcgtttg tccgcacttg ttcgggcaga tagctgccag
     5941 taccaataat cttcgtatac atgtacgctc agtcactttt cggttatata ccgtcacttg
     6001 caaactgcga gttcgctggc agcgtcctgc taccgcagag ttccgctttt gccaccgtcc
     6061 agcagctcaa aaccagctgg gtatacagat tccaggcgag cggcaattcg ctgaggaact
     6121 tgtcgctgca ccgcctgcac tgcctgttca atcgcgaccg caaaagctcg ctgattggct
     6181 gcaccatgac tttttatcac cgtgccgcgc aatcctaaca gacaggcgcc gttatactgg
     6241 tcggggttga ggtgactgaa tcgcctcgtc aggctctttt gtagccaacg ctttaataac
     6301 agtagccacc acgaccgttt tttcccttca ccctgagatt tcagcagaga aaggaacatc
     6361 ctgacaacac cttccatcgt ctttaacgtg acatttcctg taaagccgtc acaaaccagc
     6421 acatctgtct tgccagttaa caactcattg gcttcaagat agccgatata attgatagaa
     6481 gggattgttt taagcaccgc tgaggcatcc cgaatactgt caagaccctt tacttcttct
     6541 tcaccaatat tgagcaacgc cacgcgagga ttgggaattt ccaccacctc ttcagccaga
     6601 actgagccca taatggcaaa ttgcaccaac attgtgctgt cacaatcgac gttggcccct
     6661 aaatcgagga ccaccgtttt gcccttttgc tgatgtggta ataccgtcac cagcgccgga
     6721 cgctcaatcc cctccagggg cttgagtaat aattttgcca gccccatcag tgccccggta
     6781 ttaccggcac tgacacaggc ttgcgctcga ccttctttca ccagctccag ggccatacgc
     6841 attgaactcc cacggctggc gcggatagct tgcgaaggcc gggcatcact ggcgataact
     6901 gactgcgcag gaataatctg cagacgcgaa cgttgttcaa agtcagcttt agcaagtaat
     6961 ggcgtgatgg cgtcgggatt gccgactaaa agaagagtga gttgcgaatt agaattcagt
     7021 gcctgcaatg ctgcaggcac tgtcacggaa gggccaaaat cccctcccat gacatctaac
     7081 gccagggtta gacgtgtcaa ggtatcgtcg ccgcccggtt tggtctttcc ccagttgcct
     7141 ggggaaaatc ctcactaagc ttcatcacgc agacgcgtga ttacttagcg atgaccttgc
     7201 ggccgcggta gtaaccgtcg gcagtgatgt ggtgacgcag gtgtttttca ccagaagttt
     7261 tgtctacaga caggctggtg actgcggtca gcgcgtcatg ggaacgacgc atgccacgtt
     7321 tggaacgggt tggtttattc tgttgtacgg ccatggacct tactcctcaa ttacggttta
     7381 tccccgatcc aacggggagc accaattact tacgctttaa gctggctaat acggcaaatg
     7441 ggtttggctt ttgcacttct tcaggcagtt caccaaagac catgtccgct tcggacactt
     7501 cacagtgttc agaatcatgc accggaacta ccggcaaggc gaggatgatt tcatcttcaa
     7561 ccattgcaag cagatcgatt tcaccgaatt cgttaacctc aatcggttca tacgcttccg
     7621 gcagtgcttc agcctgttcg tctgaacgca caggactaaa acaatacgtt gtgtagacct
     7681 gatgagtaaa cggcttcccg caacgctgac actcgagcgt taccgtcacc ttcgcatcgc
     7741 cgtttaacac tgcgagacgt tggttatcga tagcgaacga catggagcat tccacatcac
     7801 tgtccacact gactacggat tcggcgacgc gctcaacctg atcaggggta tagataccct
     7861 ggtaatcaag gcgtttttga gccgtacgaa ccggatcgag agtcaggggt aattttacct
     7921 tttgcatagg gcgcgcatat taactttgta acgtcataga gtcaaagaaa aaggcagcct
     7981 gtggttgcct tttgccaata attcgcacac attgcgggtt actgctttat tttcgttcag
     8041 tgctgataac gaacatcaac ctgcaaaacg aagcgcatag tttaaaatgg ctatcatcaa
     8101 tacgctatat ctgatggaaa aaaatatgcc taaacttatt ttagcctcca cctcgccatg
     8161 gcgtcgcgct cttctggaaa aactgcaaat ctcttttgaa tgtgcagcac ctgaggtcga
     8221 cgaaacccca cgcagcgacg aatcaccgcg acagttggtg cttcgactgg cacaagaaaa
     8281 atcgcaatct ctggcgtcac gttatccgga tcatttaatt attggttcag accaggtatg
     8341 tgttcttgat ggcgaaatca ccggtaaacc gttaacggaa gaaaatgccc gtctgcaatt
     8401 acgcaaagcc agcggcaata tcgtcacctt ctatactgga ctggcgttgt ttaattcggc
     8461 gaatgggcat ctacaaacag aagtggagcc ttttgacgtc catttccgtc acctaagcga
     8521 ggcggagatt gataattacg tgcgtaagga gcatcccctg cactgcgcgg gtagctttaa
     8581 gagtgaagga tttggcatta cgctgtttga gcgtttagag ggacgtgacc ctaacacgct
     8641 ggttggttta ccgctaatcg cgctgtgtca gatgttacgt cgggaaggga aaaacccgct
     8701 gatgggatag ttgtttgcgc cggatgcggt tacgtcgcat ccggcaatcg gtgcaggcgg
     8761 cctgatgcgg cgtaaacgcc ttatcaggcc aaccgcccat tatcaggctt atatgcttgt
     8821 attagcgcgc gttacgcagc ttttgcaaac aacgcttcaa accttcatcc atcggcgctt
     8881 cgatacgcat cacctcaccg gtccccggat gggtaaactt caacgctgcg gcgtgcagga
     8941 acagacgatt taatcccgtg cctgcttcag tgagctgtct gtcaaattca cggtcaccgt
     9001 agcgatcgtc aaaggcaatc ggatgacccg catactgtgt atgcacacgg atctgatgag
     9061 tacgtccggt taccgggcta caacgcacca gggtggcaaa tgcatagcgt tcttccactt
     9121 taaagcgtgt ttccgacggt ttgccttcct gactgacacg cacgatacgt tcgccgcttt
     9181 gcagaatatt tttcaataac ggcgcttgaa cgctcttcac atgcgactgc cactgaccgc
     9241 gcaccagcgc caggtaatct ttttgcattc ctttttcacg taattgctca tgcagagaac
     9301 gcaacgccga gcgttttttc gctaccagca acacacctga agtgtcccgg tcgagacgat
     9361 gaaccagttc aaggaaccgc gcttccgggc gcaacgcccg caaaccttcg ataacgccga
     9421 agcttaaacc actgccgcca tgtaccgccg taccggaagg tttattcagc accaggatat
     9481 gatcatcttc atataagatg acgtccgcca gcgccgccac cttttgcaga tgtggcgaaa
     9541 ccgcctcttc ttcccgctca gcaacgcgaa ccggtgggat acgcacctca tcacccgctt
     9601 cgagtttata ttcaggctta atacgttttt tgttcacccg cacttcacct ttacgcaaaa
     9661 tacggtaaat catacttttt ggtacgcctt tcaattgggt acgcaaaaag ttatcgatac
     9721 gttgccccgc ttcgtcagcg gtgatagcaa caatttttac ggatggagtc tctgttttca
     9781 tggtgggcga ttctaattag ccaacaggat tcgcgccact catttttcta tgcttatatt
     9841 tagttttgca ccttattact tcactacgtg atcactttat tgatggttat taaaccaatc
     9901 accagcaaga agtgaaaaaa ctgtgagtaa gcgggtgata aatggtaaaa gtcatcttgc
     9961 tataacaagg cttgcagtgg aataatgagg ccgtttccgt gtccatcctt gttaaaacaa
    10021 gaaattttac ggaataaccc attttgcccg accgatcatc cacgcagcaa tggcgtaaga
    10081 cgtattgatc tttcaggcag ttagcgggct gcgggttgca gtccttaccg gtagatggaa
    10141 atatttctgg agagtaatac ccagtctgtt tctttgataa ttgcgctgtt tttccgcatg
    10201 aaaaacgggc aaccgacact ctgcgcctct ttgagctgac gataaccgtg aggttggcga
    10261 cgcgactaga cacgaggcca tcggttcaca cccggaaagg cgttactttg cccgcagctt
    10321 agtcgtcaat gtaagaataa tgagtaagtt acgatgaaaa gaatgttaat caacgcaact
    10381 cagcaggaag agttgcgcgt tgcccttgta gatgggcagc gtctgtatga cctggatatc
    10441 gaaagtccag ggcacgagca gaaaaaggca aacatctaca aaggtaaaat cacccgcatt
    10501 gaaccgagtc tggaagctgc ttttgttgat tacggcgctg aacgtcacgg tttcctccca
    10561 ctaaaagaaa ttgcccgcga atatttccct gctaactaca gtgctcatgg tcgtcccaac
    10621 attaaagatg tgttgcgtga aggtcaggaa gtcattgttc agatcgataa agaagagcgc
    10681 ggcaacaaag gcgcggcatt aaccaccttt atcagtctgg cgggtagcta tctggttctg
    10741 atgccaaata acccgcgcgc aggtggcatt tctcgccgta tcgaaggcga tgaccgtacc
    10801 gaattaaaag aagcactggc aagccttgaa ctgccggaag gcatggggct tatcgtgcgc
    10861 accgctggcg taggcaaatc tgctgaggcg ctgcaatggg atttaagctt ccgcctgaaa
    10921 cactgggaag ccatcaaaaa agccgctgaa agccgcccgg ctccgttcct gattcatcag
    10981 gagagcaacg taatcgttcg cgccttccgc gattatttac gtcaggatat cggcgaaatc
    11041 cttatcgata acccgaaagt cctcgaactg gcacgtcagc atatcgctgc attaggtcgc
    11101 ccggatttca gcagcaaaat caaactgtac accggcgaga tcccgctgtt cagccactac
    11161 cagatcgagt cgcagatcga gtccgccttc cagcgtgaag tccgtctgcc atccggtggt
    11221 tccattgtta tcgacagcac cgaagcgtta acagccatcg acatcaactc cgcacgcgcg
    11281 acccgcggcg gcgatatcga agaaactgcg tttaacacca acctcgaagc cgccgatgag
    11341 attgctcgtc agctgcgcct gcgtgacctc ggcggcctga ttgttatcga cttcatcgac
    11401 atgacgccag tacgccacca gcgtgcggta gaaaaccgtc tgcgtgaagc ggtgcgtcag
    11461 gaccgtgcgc gtattcaaat cagccatatt tctcgctttg gcctgctgga aatgtcccgc
    11521 cagcgcctga gcccatcact gggtgaatcc agccatcacg tctgcccgcg ctgctccggt
    11581 accggtaccg tgcgtgacaa cgaatcgctg tcgctctcta ttctgcgttt gatcgaagaa
    11641 gaagcgctga aagagaacac ccaggaagtt cacgccattg ttcctgtgcc aatcgcttct
    11701 tatctgctga atgaaaaacg ttctgcggtg aatgcgattg aaacgcgtca ggacggtgtt
    11761 cgctgcgtga ttgtgccaaa cgatcagatg gaaaccccgc actaccacgt gttgcgcgtg
    11821 cgcaaagggg aagaaacgcc gaccttaagc tacatgctgc cgaagctgca tgaagaagcg
    11881 atggcgctgc cgtctgaaga agagttcgct gaacgtaagc gtcctgaaca acctgcactg
    11941 gcaacctttg ccatgccgga tgtaccgcca gcgccaaccc cagctgaacc tgccgcgact
    12001 gtcgtagccc cagcaccgaa agctgcaacg gcaacaccag cagctcctgc acaacctggg
    12061 ctgttaagcc gcttcttcgg cgcactgaaa gcgctgttca gcggtggtga agaagccaaa
    12121 ccgaccgagc aaccaacacc gaaagcagaa gcgaaaccgg aacgtcaaca ggatcgtcgc
    12181 aaacctcgtc agagcaaccg ccgtgaccgt aatgagcgcc gcgacacccg tagtgaacgt
    12241 actgaaggca gcgataatcg cgaagaaaac cgtcgtaatc gtcgccaggc acagcagcaa
    12301 actgccgaga cgcgtgagag ccgtcagcag gctgaggtaa cggaaaaagc acgtaccacc
    12361 gacgagcagc aagcgccgcg tcgtgaacgt agccgccgcc gtaacgatga taaacgtcag
    12421 gcgcaacaag aagcgaaggc gctgaatgtt gaagagcaat ctgttcagga aaccgaacag
    12481 gaagaacgtg tacgtccggt tcagccgcgt cgtaaacagc gtcagctcaa tcagaaagtg
    12541 cgttacgagc aaagcgtagc cgaagaagcg gtagtcgcac cggtggttga agaaactgcc
    12601 gctgccgaac caattgttca ggaagctcca gctccacgca cagaactggt gaaagtcccg
    12661 ctgccagtcg tagcgcaaac tgcaccagaa cagcaagaag agaacaatgc tgataaccgt
    12721 gacaacggtg gcatgccgcg tcgttctcgc cgctcgcctc gtcacctgcg cgtgagtggt
    12781 cagcgtcgtc gtcgctatcg tgacgagcgt tatccaaccc agtcgccaat gccgttgacc
    12841 gtagcgtgcg cgtctccgga actggcctct ggcaaagtct ggatccgcta tccaattgta
    12901 cgtccgcaag atgtacaagt tgaagagcag cgcgaacagg aagaagtaca agtgcagccg
    12961 atggtgactg aggtccctgt cgccgccgct gtcgaacctg ttgttagcgc gccagttgtt
    13021 gaagaaatgg ctgaagtcgt agaagccccc gtaccggttg ccgaaccgca accggaagtg
    13081 gttgaaacga cgcatcctga agtgattgcc gccgcggtaa ctgaacagcc gcaggtgatt
    13141 accgagtctg atgttgccgt agcccaggaa gtcgcagaac acgcagaacc gatggttgaa
    13201 ccgcaggaag agacggcaga tgcagatatt gaagaagttg ccgaaactgc tgaggttgtg
    13261 gttgctgaac ctgaagttgt tgctcaacct gccgcgccag tcgtcgctga agtcgcagca
    13321 gaagttgaaa cggtggccgc ggttgaacca gagatcaccg ttgagcataa ccacgctacc
    13381 gcgccaatga cgcgcgctcc ggcaccggaa tatgttccgg aggcaccgcg tcacagtgac
    13441 tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg caggtggtca tacggcaacg
    13501 catcatgcct ctgccgctcc tgcgcgtccg caacctgttg agtaataatt agctcaaagt
    13561 aatcaagccc tggtaactgc cagggctttt ttatttcatg tttgaatcaa catattaaaa
    13621 ttcatcaaat ggcatccttg ctaaccaaca atgcaaaata ggcaaaaaaa atgcccgccc
    13681 cggcgatccc agagcgggca gacaaaacat acccagtttc atgatatgtt ttaaagcgag
    13741 cttatttgtt gagctggaag agcgacaatc cctgcatatc ggtaaatgct ttatacgatg
    13801 cctgcaatgc cgtttgctgc atgatgtaag atgaaatagt tgcattccag tccacatcaa
    13861 ccagatcgct catctgctgc gtttgcccta aagcgcgatc gctacctaag gaatccagcg
    13921 actccagttt gttcagctgc gtgcctaatt ccgcgcgaac ggtaaggaca ttattcagcg
    13981 agtttttcag tccacggttg gttttatcca atgccgcagc ggcgatttct ttatccgctt
    14041 cgctatccgc gaccggcgtt ttcagggctg cgatggcgct atccagcatg gcaaaaagat
    14101 tggtttcaga agcgctaccg tctggttccg ctaccgcgtt actggtaata ctgtcgaaaa
    14161 ttttgtcgcc cgtatgtcct atcaccatcg aacgcgaagc atcgacctgt tgcttaatac
    14221 tttctgttcc gccaacataa tcaccgttag cctcactaaa cggcgcagtc tctgttttat
    14281 atgtagcgcc cattaccgtc agtggtgttc gccagattca gcaactggtc acgaagcccc
    14341 tgaatatccg tagccagcga ggcccggtca tcgtcactca aggtgccatt gctggcgtag
    14401 acaatttttt cctgagcatt ctggatagca gtggtgacct ggctaagtac actctcttcc
    14461 agtgacactt tttgagtggc gaaagtacgt gccagcgtgt actggctgtt ttgcgcctgt
    14521 gcctgggaga gaacaacagc ttgtgatgca gcaatgggat cgtctgaagg gttaacgact
    14581 cgcttacccg tcgacatctg ctcgccgtac ttcatccatt ctgcctgaga attggtgatg
    14641 ccgcgcatgt tttgctggta catcatctgg gtactgaaac gcattttatc tccccttagc
    14701 gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg cagaacctgc gcattcgcca
    14761 ggtaatactg ctgaaaacgt tgcagatttc cgtactcctc atcgagattg acaccggaaa
    14821 tcgactgctg ctgattggaa agctgcgtca ccacattacc ttgcgtggtg ctactggttt
    14881 tcaacgtcgc ggttttatta ccgatatcac tcactaacga ggcataagcg tcgttaaagg
    14941 atttcgcacc gcccaccgtt ttactgttgc tttgcagatc cagcagggcc tgaccgttgc
    15001 ggttgtcgct atcacccgca tcttcttcgc tcgccatcgc gattttcgct tcgtcggtga
    15061 ttaatacatc catgttgacg atggcgtcac ttactggttt cagcgtgaag ctgtcgttaa
    15121 cggcaggcgt tcctgtaaac gtcaactcca gaccatcaaa tgccacttta ccgttggcat
    15181 ctggtgtcgc cgtaaaagtg gtattgctgg caaggcgggt gacctgccac tgattattat
    15241 cgaacgagat tttgtaatct gtcgccagta ccacggaggc atcagttacc gtggcaccga
    15301 tcgcaacgtc acctttgttt ttcgtgtttt gcagaaccgc gggcttaccg atagtaaaga
    15361 aatcttcacc agcatcgccg ttagcatcaa atccggcttt gtgttggctg ttgaaagcct
    15421 cggcaaatgc cagcgccagt tgtccaagcg tattacgcgt ctggtccaga tcctgagaac
    15481 ggaatgtcag aatgccgccc agcgacccgg tattcagtaa tttctccggg atctcaatat
    15541 tgcctgccgt cccatcaacg taagcgacag tcgtacgaga agggtcggcg ctggaaggaa
    15601 ctgccgccag ttgccgcgcc gtacttccct gaaccagtga gtaaccattg gcatcgtgat
    15661 gttataagta ccgccatcct gaacgctgac ttctacgcca acaatctggt ttaattcgct
    15721 caccagttga tcgcgttgat ccagcagatt gttaggtgac gcccctgccc ccacgcctgt
    15781 caggcgcgag atttgatcgt tcaggctggc aatttgttta gcgtaattgt tgatctgatc
    15841 aacgctggca cctatcgcga tattgacctg tttgtcctgg tcgcgcagat attgatcggt
    15901 ggttttaaac tgattcacca acccttctga tttcccaatc agcgcctggc gcgccgccgg
    15961 gtcttccgcg ttactcacca gcgtttgcag gctggtgaag aaatcctgca tctgtgttgc
    16021 cagcgaagag gtactggtgg agagcatatt gtcgattttc gacatctgct catagcgggc
    16081 ggtcaggccg ctactttgcg tctgcgcagc acgtaactgg ttggtaataa acgtatcata
    16141 ctcacgctgc acaccagaaa cgtagacgcc attgccaacc cagccgccag cgcccaacgt
    16201 gctattggcc tgcgccataa tagtggtttg gcgggtatat ccggcaacgt tatagctgga
    16261 gatattatta cttgccgtat ttaacgccgc ctgggccgcg ttcagtccgc tcatggcgtt
    16321 attgatcaag ctggacatgg aggttcctta taagccttca attacagagt attatcggca
    16381 gcgacccgcc ggacttgagt tattcagaac agattatcaa tgttcatgct gtaggttttg
    16441 ctcaccttgt cgcttatcga tttcatctgc tggatcatgt tggtgagttt acgggcatag
    16501 tgaggatcgg tagcatagcc cgcgtcctgt aggacctgcg ccccctgttc cgcactcgcg
    16561 gcggtcgtca cggcggcgta gcgcgggtta cgtgttaaca gcccaacgta atccgacaat
    16621 gcttccagat acgagctgta gacccgaaac ttcgctttta ctttcttcgc ttcgccgttt
    16681 tcatattcag tcgtggtgat ttcagtgact tgccctttcc agttgccaga ggctttgaca
    16741 ccaaacaggt tatagcttgg ctcgccgttt tcgcggcgga tttgccgttg cccccaacca
    16801 gattcaagcg cagcctgagc gaggatcaaa tgatgtggca caccgctttg ctggcttgcc
    16861 agttgggcgg gcagcgagag ttgcgcgagg aatgctctac tgtcacccgg cagcgaatca
    16921 tcgtagttac gtggtacggc cttttgcacc agctgcgaaa gcgtctggtt ttgataacgc
    16981 accacggttt cgagcgggaa tttcatcggt gctgctggcg tggactcctc tggcaatggt
    17041 tgttctggcg tcatctgttt aaccatcatc tctgcaagcc ccagaccttt gcccgccgtc
    17101 atctgttggg caatttgctg gtcatacata ctggtataca gacgagtgtg ctcgctgctg
    17161 aacaggccat cttttggtaa agcgtcgcgc atgcttttca acatcatctg cacgaacatc
    17221 ccttccacct gacgggccac cggacggata tttgccgccg gatcttcgct cgctttcgcc
    17281 tttagttcgt tgagtgattg cgcatcccag gccgcacttg ccagtagttt gctgtcgctg
    17341 atcatcagat gatttccagt tttgcccgca gacatcccgc actttgcatt gattgcaaaa
    17401 tagacatcag atccatcggc gtagcgccca gcgcattgag cgcacgcacc acgttattga
    17461 ggctggcgct ggaacgtacg ctttgcagcg aaccgccgct ctggcgtaaa tcgatctgcg
    17521 tttgtggagt aacaacagtc tgtccaccac caaacggtgt atctggctgg ctgacattgg
    17581 cctgacgatt aactgttact gagagatttc cctgcgctac cgcgcagctg tcgagggtca
    17641 cttcgcgatt catcaccacc gaaccggtgc gcgagttaat cactacttta gcgtcctgcg
    17701 gggtgacatt aacctgcata ttctggatat cggcaaggaa acggacctgg gaactgttgc
    17761 cacttggtac gcgcacctga atagtccgcg catctaacgc ggtggcgctg ccatatccac
    17821 gcacgcggtt gatggtgtca gcgatttgct gcgccatgct gaaatcttcg tcgttaagtt
    17881 gcaaattaag ggtattcccg acgccaaact ggctgggcaa ttcacgttca ataaccgcac
    17941 cattggtgat ccgtccaccg ttcagttggt taacctgaac actgctaccg cctgcggagg
    18001 ctcctgcgcc gccaaccaga atattgccct gcgccagcgc atacacctga ctgtcaacgc
    18061 ccttaagcgg agtcatcaac aacgtacctc cacgcaagct tttggcatta cccatggaag
    18121 aaaccaccac atcgatggtt tgcccctgac gtccaaacgg aggaagtgac actgtcacca
    18181 ttaccgcagc gacgtttttt agctgcatat tggtgcccgt cggaacggta attcccagct
    18241 gtgagagcat gttattaagc gtttgtgtgg taaacggcgt ctgggttgtc tggtcaccag
    18301 tgccatccag ccccactacc aagccatagc caatcagtga gttttgcctt accccctgaa
    18361 cactggtgag atcgcgaata cgctcagcct gagccgccgt cgtaaccagt agaagaatta
    18421 atgcagagag aaatttaatc accacagcct cacttacatt ggcgacaggt taaggaagaa
    18481 acgctgcaac cagcccatat tttgcgcttc gttaatgtag ccattgccta cgtattcaat
    18541 acgcgcatcc gccacctgag tagacggtac ggtattgctg ccgctgatag tgcgtggatt
    18601 aaccacgccc gagaagcgaa taaattcggt accctgatta atggcaatct gtttttcacc
    18661 caccacatgc aggttgccgt tgaccagtac ctggtcaacc gtcaccgtca acgtgccgct
    18721 aaaggtattg ctggcattgg ccccgccctt tccgttgaac gtgttaccac cggaggcttc
    18781 gacatcggca cgagcgttac caaacagccc ctgcaaatag cgcggcacag tatcaaagcc
    18841 aaaattagtt ttaccgtcac ggctggcatt cgcagaggag cttttgctgg cgctgacgtt
    18901 ctcctgcaac acgatggtca gcgtatcgcc aatattgcgt ggtcgacgat cttcaaacag
    18961 cggttgatag ccatagttaa tcggctgagc agactggaaa atagaaccgt tggcgacggg
    19021 cgtcggaccg ggaaccggtt gtgcactggt cgccccctgc accagcggcg tggagggtat
    19081 ccaggcgcag ccggttagtg aaagcaccaa caagctggaa atggcataag tatgcgcagc
    19141 gtttttttgc atggctatca tcttcaaaaa tcagtaaacc ggtgaacctg ccaccggtta
    19201 tgccttagag ttgcgtcagt ttttgcagca tctgatcggt ggtggacacc gctttactgt
    19261 tgatttcgta agcgcgttgc acctgaatca tattgaccag ttcttccgcc acgttgacgt
    19321 tagacgtttc aacataccct tgatacagca gtcccgcgcc gttcaggccc ggcgtgcttt
    19381 cgttcggtgc accggaggat tgcgtttcgg tgtagaggtt ttcgccaatg ctctccagtc
    19441 cggtatcatt cataaaggtg gtgagattga gctgcccaac ctgaaccgga gctgcctggc
    19501 cttgttgggt tacgctgacc acgccatcac gaccgatggt gatacttaac gcattcgccg
    19561 gaatggtgat cgctggctgc acctgaaaac caccagccgt caccagctgc ccgttctgat
    19621 ccacctggaa agagccgtca cggatatagg ctgatgagcc atctggcaac attacctgga
    19681 aaaagccctg ccctttaatc gcgacatctt tgctgttatt ggtctgcgac agattgccct
    19741 ggctgtgtaa gcgttcagtg gcgaccgggc gtacccccgt gccgatttgt aatccggagg
    19801 gtaaggtggt ttgttcggaa gactgtgccc ccggctggcg aatggtttga taaagcagat
    19861 cttcaaacac cgcgcgctga cgcttaaaac cgttagtact gacgtttgcc aggttgttgg
    19921 caatgacgtc catattggtt tgctgggcgt caaggcccgt tttggcgatc cataatgaac
    19981 tgatcatata atgtcctgta gtggattgct gacaagcaga gcagaagcac gcgacagtgt
    20041 gaatttcccc tcaccctaac cctctcccca aaggggcgag ggaatagtcc gcgcgctttt
    20101 attgcaatgt catcaatcgt tcaattaact catcgacagc agttggttgg cacggcctgc
    20161 gttatcatcg acgctgctga tcaccttcat ctgcatttca aaacgccgcg cgctggcaat
    20221 catgtcgctc attgccgcaa cggcattgac gttactgcct tccagaaccc ccgacatcac
    20281 acgcaaggtt ggatctgcct gcagtaccgg ccctcgcgtg gcctgggttt ctgcgcttaa
    20341 acgaaaaatg ccgtcgtcac cgcgctgcac ttcgctgccc gtggctttca ccagtttcag
    20401 acgccctact ggcgcaactg tatttgccgg atcgcccgga ttgagcgccg agattgtgcc
    20461 atcggcagca atcgtgattt ccgccccttc cggcacagca attggccccg cctcgcctat
    20521 caccggatgc ccctgaattg tcagttgccc ggtgggatca acctgaatgc tgccattacg
    20581 cgtataccct tcgctgccgt cagcggtctg cacggccagc catcctgctg caacgcaacg
    20641 tccagcgggc gagaggtgta atccatcttg ccgggcgtca tgtctgcacc cggtgttgac
    20701 gccgttacca gcgtgcgcgt gggcagtgaa agcccttcca ccggcactgc gcgcaaggcg
    20761 tttaactgcg cgcgaaaacc gggcgtagag gcattggcca gattactggc ggttaccgcc
    20821 tgttgattca gcgtctggct ggctgctccc atcgcagtat aaattgcgtg atccattgag
    20881 ctatcccgtc agcgattagc gtaagttaac ccgcgtgttg aggatctggt cctgggtttt
    20941 gatggtctgg gcgttagact tatagttacg ctgggcaacg atcatattga ccagttcttt
    21001 actgagatcg acgttggacg cttccagcgc accgttggtc agagtgccaa agtttcccgt
    21061 cccggctgtc cccaacagcg ccacgccaga agattgcgtc gcagaccaga cgttgtcgcc
    21121 ttcggatgcc agaccttcgt tgttggcaaa gttcgccagt acaatctgcc ccagcagttg
    21181 ggtttgttcg ttggaattgt tgccgacaac cgtaccgtca tcattgattt gataactcac
    21241 cagatcgccc ggtttgtagc cattctgggt ggttgccaca atgttgttag cgccggtatt
    21301 ttgctgcatg gagttgagga agctcagact aaacgtggcg ggttctgcac cgttaattgc
    21361 gccggttgcg atattattcg ccatcgcacc atccactaat gtgccattag cattaaattc
    21421 cagtgttgtc gctgtcttcg caatgctgtt tggatcactg ctatcctggg tgtagacctg
    21481 ccagttatta tccccggtct tcacaaaata gacgctcatg tcatgagcat taccctgact
    21541 gtcgaaaaca gtcaccgaac cttttttgtt atagctatcc gcattgctgg cgctgaatgg
    21601 cgtaacagta ggaagcggat cactggaatt caggttgatc tgcatcgacg ccgtggtggt
    21661 agttttcgct gccatcaggg tattcgggat cgaaatattg gtcggattcg ccccttgctg
    21721 aatagtcggc ggcgtaccgg ttaccgggta acccgtcagc tgtaaacctt gcgtattcaa
    21781 caggttacgg ttttcatcca gcttaaattg tccgttacgg ctgtagaaca ccgaaccgtt
    21841 gctgtctacc agacggaaaa aaccgttctg gctgatagca acgtccagac ctcgcccggt
    21901 gttggtggtc gtgccatcgg taaagtcctg agtgataccg gcaactttta cccccagtcc
    21961 cactttcgaa ccggcaaaca tatcggcaaa agaggccgtg cctgatttaa agccgtaggt
    22021 ggcggagttg gcgatattgt tgccaataac atcgaggttg gtggcggcag cgtttaatcc
    22081 gctaaccgct tgagaaaagg ccatgactga ctcctgaaat gtgaaggctt aaattatctg
    22141 ccgtacttcg tcgagggtgg tggtgccgta agtgccgaga tccagcgtat taccgctgtt
    22201 gccgcggatc acaccctgca ccagagcaaa ctgcagcggc tgggcaacca gttgtgtacc
    22261 accgttactg gcgctaatcg ctacattgta agaaccgttc ggcgcagtgc tgccatcagt
    22321 caacgaaccg tcccaggtga aactgtgaac tccggcggtc agttcaccaa tatcaatggt
    22381 gcgcacaacc gcgccatttt tatcggtgat ggtggccgta actttgtctg ccgcctgttg
    22441 cagctcaaca ccaaacggcg tggtcgtggt cacagcccct tcttcactgc cggttcccgc
    22501 aagaacagtt gtgccgggga tcatcacgcc gtgaccgatc aggttactgg cctgtaacga
    22561 ctggctgtta tcaatctgtc cggaaataga tccgagcgtg gtattgagtt tttcaatccc
    22621 gctgaccgtg ctgatttgtg ccaattgcga cgtcagctcg ttgttttcca ttggattggt
    22681 cgggtcctgg tttttcagct gcgccaccag caaagtcaga aaactgcttt gtaaatctgc
    22741 ggcgttgctg cccgtgagcg aactactgct ggtggtactg acgccggtat ttgtcggatc
    22801 ggtggtggtt accgcaatgg acatagcttt ctcctttatt gaccgagcgt aagggttttc
    22861 agcatcatgc ttttcaccgt gttgagcact tcaacattgg cctgatagct gcgtgacgcc
    22921 gacatggtgt taaccatctc cccgacgaca tcaacgttcg gcatttttat gtagcccttt
    22981 gcatctgcca gcggattacc tggttcataa accagtttgt ccggggcctg actttcttaa
    23041 catcggcaac ctttacgccg cctgtcgcag cacctggtgc agcgttaacc tggaatacca
    23101 cctgttttgc ccgatatggc tgtccatcag gaccggtcac gctatcagca tttgccagat
    23161 tactggccgc cacgttcagg cgctgggact gggcagttaa cgccgacccg gcgatatcaa
    23221 aaatattcag cagtgccatc cgttaatttc cgctctgtaa aacgttcatc atgcctttga
    23281 tttgcccgct caacgcgcta aggctcatct ggtattgcag gctgttatcg gcaaactggg
    23341 tgcgttcgcg atccatatcg acggtattac cgtcaagcga aggctggtcc ggaatacggt
    23401 attgcagttc tgcggtagga ggcgtcagcg cctgcgccgg gatgtgctgc gttgaggtca
    23461 tcgtcagtgc aaccacactg gttgcatccc gtccacgttg catgactttt ttaagttcac
    23521 tggcaaaatc gatatcgcgc gcctgataac caggggtatc ggcattggcg atgtttgctg
    23581 ccagcacttc ctgacgctgg gcgcgcagat tgagcgcctc ttgttgaaaa cgtaaggcgg
    23641 cgtcgagctt atcgagcata tctcctccgc aggtatcaaa attctgccat cagcttaaat
    23701 gcctttacgc gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt tatgccgttg
    23761 atggtcattg cggacaggta caattcacgt tgtagaaatg gctgggggcg aaaatgctga
    23821 caataaaacg tagcgtggcg atcatcgcga tactgttcag tccgttaagt acggcgagca
    23881 atctcacatc gcaattgcac aactttttta gcgcccaact cgcgggggta agtgatgagg
    23941 ttcgtgtttc tattcgtaca gcgcccaatc tactaccgcc atgcgagcag ccattgcttt
    24001 cgatgagcaa taattcccgc ctgtggggca atgtgaatgt gttggcacgc tgcggtaacg
    24061 acaaacgata tttacaggtt aatgtgcagg ccacaggaaa ttatgtggtt gccgcgatgc
    24121 ctattgtgcg gggaggaaag ctggaagctg gcaatgtcaa actgaaacgt ggacgcctgg
    24181 ataccctgcc accgcgtacg gtgctggata tcaatcaact tgttgatgcc gttagcctgc
    24241 gcgatctatc acccgatcaa cctatccagt taacccagtt tcgccaggca tggcgggtaa
    24301 aagcgggaca acgcgtcaat gtgatcgcca gcggtgatgg gtttagcgcc aacgcagaag
    24361 gtcaggcgct gaacaatgca gccgtcgcac agaatgcgcg ggtgcgcatg gtatcggaac
    24421 aggtagtcag cggcgttgtt gatgcagatg ggaatattct tataaacctg taagctgtta
    24481 aagattaccc gtcccttgcc gataaataag caacacatga taaaagcgcc ctcaatgagg
    24541 aataaaccat gagtattgat cgcacttcgc ctctgaagcc tgtaagcacc gttcaaccgc
    24601 gcgaaaccac cgacgcgccg gtaacgaaca gccgggcggc aaaaacaacc gcctccacca
    24661 gcaccagtgt gacgttaagc gacgcgcaag caaaactgat gcaacccggc agcagtgata
    24721 tcaatcttga acgtgtcgaa gcgttaaaac tggcgattcg taacggtgaa ctaaaaatgg
    24781 acaccggcaa aattgccgat gcgctgatca acgaagcgca gcaagacttg cagagtaact
    24841 gatcgtatga cacgtcttgc agagatcctc gaccagatgt ccgctgtgct taacgatctc
    24901 aaaacggtaa tggatcaaga gcagcaacat ctctctatgg ggcagatcaa cggcagccag
    24961 ttgcaatgga ttacagaaca aaaaagctca ctgctggcga cgctggatta cctcgaacag
    25021 ttacgcaaga aagaacccaa tacagcaaat agcgttgata ttagtcaacg ctggcaggaa
    25081 attactgtga aaacgcagca actacgccaa atgaatcaac ataacggctg gttactggaa
    25141 ggacagattg agcgcaatca acaggcgctg gaaatgttga aaccgcatca ggaaccgacg
    25201 ctatatgggg cgaacggtca gacctcaaca ccccatcgcg gcagtaaaaa gatttcgatc
    25261 tgaatattat ctccggcctg cactgcaggc cggaatgcat tgttacaccg tccggcgggc
    25321 aaattcttta actttgaagc ccagtaccgc cagtgcagcg aagtacgcgg caatccccgc
    25381 cagcacgacc gccattaaac gcagtaagcg ccagggcatg gtacccaatg accactccgg
    25441 catgatatgt aacataccta aaagcacgcc agacatcacc agtaccgcca ccaccagacg
    25501 caacagaaac gccatccagc cgggttgcgg agtaaagatt ttctgcttac gcaactgcca
    25561 gtaaagcagc gaagcattca gacacgccgc cagaccaata gaaagtgaca gcccggcatg
    25621 tttcaacgga ccaataaacg ccaggttcat caattgcgtt aaaatcagcg taacgatggc
    25681 aattttcact ggcgttttaa tgtcctggcg ggaataaaag ccaggagcca acactttcac
    25741 tacaatcagg ccaatcaaac ccaccgagta ggcaattaac gcccgctggg tcatcagcgc
    25801 atcaaacgcg gtaaatttac cgtactggaa cagcgaaacg gttaacggac ctgaaagaat
    25861 gcccaacgca accgcactcg gcagtgccaa caggaaacaa agacgcaacc cccagtccat
    25921 caaacggttg tattcatcat gattgccact ggcaaaactt ttcgacagcg acggcagcaa
    25981 aatggtgcca agcgccacgc ccagcacacc ggagggaaac tccattaagc ggtcggcgta
    26041 atacatccaa gacaccgaac cggaagcaag aaacgaggca aaaatggtgt tgatgattaa
    26101 ggagatctgg ctcacagaga cgccaaggat cgccggtccc atctgtttca ccacgcgcat
    26161 tgctccagca tcgtggaagt taatgcgcgg caggaccagc atgccgatct tcttcaggtg
    26221 cggtagctga tacaccagct gcagaatgcc gccgaccgta acagcccacg ccagcgccag
    26281 caccggtggg ttaaagtacg gtgcggcaaa cagcgcgaaa ccaatcatgc tgatgttaag
    26341 cagtgttggc gcaaacgccg gaatcgagaa gcggttccac gtattcagaa tcgctcccac
    26401 cagcgacgcc agggagatca gcaagatata gggaaaggta atctttagta gctggctggt
    26461 cagggcaaat ttgtcagctg tgtcagcgaa gcctggcgcg gtcaccatga tcacccacgg
    26521 tgcggcgagc atgccagcga ccgtcacaac cgccagcgca agtgtcagca ggccagaaac
    26581 ataagagaca aagacccgcg tggcgtcttc accctgctta cttttatatt ccgccagaat
    26641 cggtacaaat gcctgggaaa atgccccttc ggcaaagata cggcgtaaca agttaggaag
    26701 tttaaaagcg acgaaaaagg cgtcggttgc catccctgcg ccaaagattc tggcgacaat
    26761 tgcgtctcgt gcgaagccaa gcacacgcga aaacatggtc atcgagctga cggcggccag
    26821 cgattttaat aaattcatcg gtgttctaat ccggacccac gggcaaaacg cctgcaaggc
    26881 aggcgctgaa agaggcgaat agtctaccta gattatggtg aattgctacc gccagatgtt
    26941 acagggttta ttcactcatc gcatcgcgcc agatcttgtc aacgatacgt tgcgccagca
    27001 ctgcctattc gccggcggtt tgcggaactg tctgatttta cacgcattca atgaagtgcc
    27061 gcgcacagcc gacaaatcca cgttgctcaa gagtgctctg ccaaccagga atcggtttat
    27121 tcactacacc ctgcccgcgc tcctcacgcc attcgcgcat atccgtaatg tcgatgagcg
    27181 caccgtcagt cacggcctgc acggtttcac gctgacttcc ggcacggcga tgcatacaag
    27241 tggtgatctg caaaggaccg gccgaaaaat ggtgctcggc aaacagcatt tcgccagcgt
    27301 cgttagtcag tagcgtaccg ccatccagag aggctttgcc gcccgacaac cacagcgcgg
    27361 tatccaccac atgcagataa tcatccagca acgtgaaata aagatcgtgt ggcccgacgc
    27421 tattgctacg atgtttatcc attcttagcg aggctgcggt ggcgagttgc gtttttaact
    27481 caccgtagag tggtgcgaaa cgacggttaa aaccgaccat caacgtcagt tttttccgcg
    27541 ccgccagttc caccagcctt tcagcatcgc gcagattttc tgccagcggt ttatcgacac
    27601 agacatgtac ccccgcattg agtaacgtac tgaccacgtc aaagtggctg gcggtgctgg
    27661 agtgcacaaa aaccgcatca caactggcgg caaggctgga taacgaatcg gcataaggaa
    27721 tgcgccagct gtcacaaatt ggcagggctt tggcgcgcgt aggcgaccag gctccttgta
    27781 acgtccagtc agacgctgcc gccagcaccg gtaaccacgc tttttgcaca atgccgccta
    27841 atcccactac gccgatacgt aattttttca ctttaatctc ccagatgggc cagcaacgaa
    27901 tcaagacgtt gtttcagttc tgccacttcg atttccaggg cttcgacgcg ggtctgtaaa
    27961 tcaccgtcaa ccatgttcga catagccgtt accgccggct gatcttcaac ctcaccactg
    28021 aaaagatgca tgtagcggct ttcgcgttta cccggttcgc gggccagacg caccacaaaa
    28081 ggaccatctt cgcgattcgc cagttgttcc agcgtcgact ccacttccgc catatcgctg
    28141 aattcataca ttcgcgcggc gcggctgcgc agttcacccg gcgtctgggc accacgcaat
    28201 aacaacgtgg tgattaacgc cacttctgcg gcgctcagtt tcagatcgcc aaattctgaa
    28261 ttacaaaaac gttgctcata tttggtgacc cgattaccaa aaccgctcac tgtgcgtaga
    28321 taatgacgtt tgaccagatt atccagctgt tcctgcactt cggattcact cagattcatg
    28381 accggttcac ggttcgtttt ctgattacag gccgtgacta caccattgac tgagagcggg
    28441 tattgctccg gcgtggtcac ctgcttttcc agcaagcaac caattacgcg agcttccagg
    28501 gcagtaagtt gatatttcat gagattctcc ttagcggccg ggcgtccagt ctggggtagt
    28561 taatgccgtc agtacgtgat cgcgccattg tccatcaatc aacagatagt ctttcgcata
    28621 gccttctttt tcaaaaccca gtcgcgccag taaatcaccg ctgcgtttat tgtgcggcat
    28681 ataattagcc ataatgcgat gaatatgttg ggtgcgctgc atataacgaa tggctgcggt
    28741 cagggcttca aacatgagtc ctttgccctg ccatttttgc ccaatcgaat aaccgagata
    28801 gcaggcatga aaagagccgc gaacaacatt ggaaaaattg gcaacgccaa taatctcttt
    28861 ttcgtccggg tcgaataagc caaagtagaa agctgaacct tgtttatgaa attcgttaat
    28921 catccctagc ctggcctgcc agcctgatgg ataacagtgg ctttcgtcgc gcactggctc
    28981 ccagggcttg aggaaatggc gattctctgc gtaataatcc gccagacgcc aggcatcacg
    29041 atcatgcacc agacgcacga ccagtcggtc tgtggttaag cgcacttttg gcacgttact
    29101 gcgatagcca aacattctat acctactcct tcccgtaacg tctttaatac gctgtaagta
    29161 ttactatacc tgcgcaacca gaccctgtga aaagtccaaa atgccatttt ctgcattatt
    29221 cacatatttg ataaaaactc tctatcaaag cgacgttttg ataaaaaaat actgtccccg
    29281 aagtgatggg aaaagtctta gcggcaatgc agaatagaaa gtgctcatct tttatttccc
    29341 gggaggggaa atgtcccgcg tatcgcaggc gaggaacctg ggtaaatatt tcctgctcat
    29401 cgataatatg ctggtcgtgc tggggttctt tgttgtcttc ccgctgatct ctatccgctt
    29461 cgttgatcaa atgggctggg ccgccgtcat ggtcggtatt gctctcggtc tacgccagtt
    29521 tattcagcaa ggtctgggta ttttcggcgg cgcaattgcc gaccgctttg gtgccaaacc
    29581 gatgattgtt accggtatgc tgatgcgcgc cgccggattc gccacaatgg gtatcgccca
    29641 cgaaccgtgg ctattgtggt tttcatgcct gctctcggga ctcggcggca cgttgtttga
    29701 tccgccgcgt tcggcgctgg tggtgaaact aatccgtcca caacaacgtg gtcgtttttt
    29761 ctcgctgttg atgatgcagg acagtgccag tgcggtcatt ggcgcattgt tggggagctg
    29821 gctgttgcaa tacgactttc gcctggtctg cgccacaggg gccgttttgt ttgtgctatg
    29881 tgcggcgttc aatgcgtggt tgttaccggc atggaaactc tccaccgtac gcacgcccgt
    29941 tcgcgaaggc atgacccgcg tgatgcgtga caagcgtttt gtcacctatg tcctgacgct
    30001 ggcgggttac tacatgctgg ctgtacaagt gatgctgatg ctgccaatta tggtcaacga
    30061 cgtggctggc gcgccctctg ccgttaaatg gatgtatgcc attgaagcgt gtctgtcgtt
    30121 aacgttgctc taccctatcg cccgctggag tgaaaagcat tttcgtctgg aacaccggtt
    30181 gatggctggg ctgttgataa tgtcattaag catgatgccg gtgggcatgg tcagcggcct
    30241 acaacaactt ttcaccctga tttgtctgtt ttatatcggg tcgatcattg ccgagcctgc
    30301 gcgtgaaacc ttaagtgctt cgctggcgga cgcaagagct cgcggcagct atatggggtt
    30361 tagccgtctg ggtctggcga ttggcggcgc tattggttat atcggtggtg gctggctgtt
    30421 tgacctgggc aaatcggcgc accagccaga gcttccgtgg atgatgctgg gaattattgg
    30481 catcttcact ttccttgcgc tgggttggca gtttagtcag aaacgcgccg cgcgtcgttt
    30541 gctggaacgc gacgcctgat ttgatactcc cccctgactc ctccatactg aaagtgaact
    30601 gacagccaaa tggaggagtc acgtgaagct atacatttac gatcactgcc cttactgcct
    30661 caaagcccgc atgattttcg gcctgaaaaa tatccccgtc gaattacatg ttctgctcaa
    30721 cgacgacgca gaaacaccca cccggatggt cggtcaaaaa caggttccca ttctgcaaaa
    30781 agatgacagc cgctatatgc cagaaagcat ggatatcgtt cactatgtcg ataaactcga
    30841 cggcaaaccg ttactgaccg gcaaacgttc ccctgccatt gaagagtggc tgcgcaaggt
    30901 caatggctac gtcaacaaac tgctgttgcc gcgttttgcc aaatcggcat ttgatgagtt
    30961 ttctactccc gccgcgcgca aatatttcgt cgacaagaaa gaggccagcg cgggtaattt
    31021 tgccgacctg ctggcccact ctgacggtct gattaagaat atcagcgatg atttgcgtgc
    31081 gctggacaaa ctgatcgtca aaccgaacgc cgtgaatggc gaactttcgg aagatgatat
    31141 tcagctattt ccgctactgc gtaatctgac gctggtagcc ggaatcaact ggccaagccg
    31201 cgttgctaat taccgcgata atatggcgaa acagacacaa atcaatttgt tatcatcaat
    31261 ggcgatttaa tcctgacccg ccgcggcagc ctgtattcgc gggttgccgc cgttttcagt
    31321 tttttctgct ggcgagttta tcgctggtga cacatgctgc gttgtgtcag gatattgacg
    31381 cagatttcat gtcgaggact accatgaaca agtttttatt tgccgctgca ttgatcgtca
    31441 gtggcctgct cgttggctgt aatcaactca cccaatacac catcaccgaa caagaaatta
    31501 accagtcgct tgcgaaacat aataatttct caaaagatat cggtttaccc ggcgtggctg
    31561 acgcccatat tgttctgaca aacttgacca gccaaattgg tcgcgaagag ccgaataagg
    31621 ttaccctaac cggagacgcc aatctggaca tgaactccct gttcggtagt cagaaagcga
    31681 ccatgaaact gaagctgaaa gcgctgcccg tgtttgataa agaaaaaggt gcgatcttcc
    31741 tgaaagagat ggaagtggtc gatgcgacgg tacaaccgga aaaaatgcaa acggtgatgc
    31801 aaacgttgct tccctatttg aaccaggcat tacgcaatta ctttaaccag caacctgctt
    31861 acgtcctgcg cgaagatggc agtcagggcg aagcaatggc gaaaaaactg gcgaaaggca
    31921 ttgaagtgaa gccaggcgaa attgtcattc cgtttacgga ttaatgacga gggcgcattc
    31981 gcgcccttta tttttcgtgc aaaggaaaac gtttccgctt atcctttgtg tccggcaaaa
    32041 acatcccttc agccggagca tagagattaa tgactgcacc atcccaggta ttaaagatcc
    32101 gccgcccaga cgactggcac cttcacctcc gcgatggcga catgttaaaa actgtcgtgc
    32161 cgtataccag cgaaatttat ggacgggcta tcgtaatgcc caatctggct ccgcccgtga
    32221 ccacagttga ggctgccgtg gcgtatcgcc aacgtattct tgacgccgta cctgccgggc
    32281 acgatttcac cccattgatg acctgttatt taacagattc gctggatcct aatgagctgg
    32341 agcgcggatt taatgaagcc gtgttcaccg ctgcaaaact ttacccggca aacgcaacca
    32401 ctaactccag ccacggcgtg acgtcaattg acgcaatcat gccggtactt gagcgcatgg
    32461 aaaaaatcgg tatgccgcta ctggtgcatg gtgaagtgac acatgcagat atcgacattt
    32521 ttgatcgtga agcgcgcttt atagaaagcg tgatggaacc tctgcgccag cgcctgactg
    32581 cgctgaaagt cgtttttgag cacatcacca ccaaagatgc tgccgactat gtccgtgacg
    32641 gaaatgaacg gctggctgcc accatcactc cgcagcatct gatgtttaac cgcaaccata
    32701 tgctggttgg tggcgtgcgt ccgcacctgt attgtttacc catcctcaaa cgcaatattc
    32761 accaacaggc attgcgtgaa ctggtcgcca gcggttttaa tcgagtattc ctcggtacgg
    32821 attctgcgcc acatgcacgt catcgcaaag agagcagttg cggctgcgcg ggctgcttca
    32881 acgccccaac cgcgctgggc agttacgcta ccgtctttga agagatgaat gctctgcagc
    32941 actttgaagc attctgttct gtaaacggcc cgcagttcta tggcttgccg gtcaacgaca
    33001 cattcatcga actggtacgt gaagagcaac aggttgctga aagcatcgca ctgactgatg
    33061 acaccctggt gccattcctc gccggggaaa cggtacgctg gtccgttaaa caataaaaat
    33121 cgtagcttcc tgttgtcatt aggttatttt acctgtataa ataaccagta tattcaacag
    33181 ggggctatta tgcgaattga agtcaccata gcgaaaactt ctccattgcc agctggggct
    33241 attgacgccc tggctggcga actttcccgc cgtattcagt atgcgtttcc tgataatgaa
    33301 ggccacgtat cggtacgtta tgccgcagcg aataatttat cggttattgg cgcaacaaaa
    33361 gaagataaac agcgcattag cgaaattctc caggaaacgt gggaaagcgc cgatgactgg
    33421 tttgtcagcg aataatatgc agtgattttt gtttttgccg gatcgccccg gcttttttat
    33481 tttacttacg aattttgagt acaaaatttc tacacttcct caaaataatt gcacaatatg
    33541 ctaaaaaaat atgaatgaca tgctgtttta ttgttcatat aaatcttaaa agcagaaatt
    33601 tatctgttcc cccctttaaa aattcggtga atacccttac ttattggtat actgaagttg
    33661 cttcagataa aaacgcattg aacctcgaat aacgttgtct agtaacacga attagggggc
    33721 catgatggaa aaaaataatg aagtcattca gactcatccg ctcgtagggt gggacatcag
    33781 caccgttgat agctatgatg cgctgatgtt gcgtttgcac taccagaccc caaataagtc
    33841 cgagcaggaa gggactgaag ttggtcagac gctctggtta accactgatg ttgccagaca
    33901 atttatttcg atattagaag caggaatcgc caaaattgaa tccggtgatt tcccggtaaa
    33961 cgagtatcgg cgtcattaac atgacatgac catccaccca aaaggcacct caccggtgcc
    34021 tttaccattt ctgggttgta tatctgcatt cagttaatta ctctgcttaa accatgagag
    34081 cagagagaaa aaaatgaaat acgatctcat cattattggc agcggttccg taggcgctgc
    34141 cgccgggtat tatgcaaccc gcgccggttt aaacgtgcta atgaccgacg cccatatgcc
    34201 accgcatcaa cacggcagcc accacggcga tacgcgatta attcgccatg cttatggtga
    34261 aggcgaaaag tatgtcccgt tggtcctccg cgcgcaaacg ctgtgggatg aactttcccg
    34321 ccacaacgaa gatgatccca tttttgtacg ctctggtgtc attaacctcg gcccggctga
    34381 ctccgcattt ctcgccaacg tcgcccacag cgccgaacag tggcaactca acgttgaaaa
    34441 gctcgatgcg caagggatta tggcccgctg gccagaaata cgcgtcccgg acaactacat
    34501 cggcttattt gagactgatt ccggtttttt gcgcagcgaa ctggcgatta aaacctggat
    34561 ccaactggcg aaggaagcgg gctgtgcgca actgttcaac tgcccggtca ccgcaattcg
    34621 tcatgacgat gatggcgtaa ctattgaaac ggttgacggt gagtatcagg cgaaaaaagc
    34681 gattgtctgc gcgggaacat gggtaaaaga cctgctcccg gagctgcctg tccagcccgt
    34741 acgtaaagta tttgcctggt atcaggccga tggccgctat agcgtgaaga ataaattccc
    34801 ggcgtttacc ggtgaactgc ccaatggcga tcaatattat ggttttccgg cagaaaacga
    34861 cgcgttgaag attggcaaac ataacggagg ccaggttatc cattcagcgg atgaacgtgt
    34921 tccgtttgcg gaagtggcca gtgatgggtc agaagccttc ccgttcttgc gcaatgtatt
    34981 gccgggtatc ggttgctgcc tgtacggcgc tgcctgcacc tatgataatt cgcctgacga
    35041 agattttatt atcgataccc tacccggcca cgataataca ctgctcatta ccggcctgag
    35101 tgggcacggt tttaaatttg cgtcagtttt aggggaaata gctgccgatt ttgcgcaaga
    35161 ccaaaaaagc gattttgatt tgacgccatt caggctttcc cgcttccaat aatcatcatt
    35221 acggcctcca gaatgaggcc gtttttacag gagtatttaa tgcgtcggct gttgcactat
    35281 ctcatcaata atattcgcga gcatctgatg ctttatcttt ttctctgggg attgttggca
    35341 attatggatc tgatctatgt gttttatttt tagttttccg cactgacata aactgacaaa
    35401 tatttaatct ccctttcatt tagcgtgaaa aattgaatga gattattcct gcttctacag
    35461 cactcaccag acatct
//
LOCUS       DAZXUK010000014        34971 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZXUK010000014 DAZXUK010000000
VERSION     DAZXUK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34971
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.014"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1316
                     /gene="argA"
                     /locus_tag="ACH3L8_000582"
     CDS             <1..1316
                     /gene="argA"
                     /locus_tag="ACH3L8_000582"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFT4083831.1"
                     /translation="TELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIG
                     LLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAGTLQLDI
                     TARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDALHRQLE
                     SGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSE
                     LFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFS
                     RDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEIDKFTII
                     QRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQSGLSKLF
                     VLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(1378..3204)
                     /gene="recD"
                     /locus_tag="ACH3L8_000583"
     CDS             complement(1378..3204)
                     /gene="recD"
                     /locus_tag="ACH3L8_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFT4083832.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(3204..6746)
                     /gene="recB"
                     /locus_tag="ACH3L8_000584"
     CDS             complement(3204..6746)
                     /gene="recB"
                     /locus_tag="ACH3L8_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFT4083833.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(6739..9627)
                     /gene="ptrA"
                     /locus_tag="ACH3L8_000585"
     CDS             complement(6739..9627)
                     /gene="ptrA"
                     /locus_tag="ACH3L8_000585"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFT4083834.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(9803..13171)
                     /gene="recC"
                     /locus_tag="ACH3L8_000586"
     CDS             complement(9803..13171)
                     /gene="recC"
                     /locus_tag="ACH3L8_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFT4083835.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(13184..13534)
                     /locus_tag="ACH3L8_000587"
     CDS             complement(13184..13534)
                     /locus_tag="ACH3L8_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFT4083836.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(13519..13926)
                     /locus_tag="ACH3L8_000588"
     CDS             complement(13519..13926)
                     /locus_tag="ACH3L8_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFT4083837.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(13923..14486)
                     /locus_tag="ACH3L8_000589"
     CDS             complement(13923..14486)
                     /locus_tag="ACH3L8_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFT4083838.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(14477..14947)
                     /locus_tag="ACH3L8_000590"
                     /pseudo
     CDS             complement(14477..14947)
                     /locus_tag="ACH3L8_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(15131..15925)
                     /gene="thyA"
                     /locus_tag="ACH3L8_000591"
     CDS             complement(15131..15925)
                     /gene="thyA"
                     /locus_tag="ACH3L8_000591"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFT4083839.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(15932..16807)
                     /gene="lgt"
                     /locus_tag="ACH3L8_000592"
     CDS             complement(15932..16807)
                     /gene="lgt"
                     /locus_tag="ACH3L8_000592"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFT4083840.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(16958..19204)
                     /gene="ptsP"
                     /locus_tag="ACH3L8_000593"
     CDS             complement(16958..19204)
                     /gene="ptsP"
                     /locus_tag="ACH3L8_000593"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFT4083841.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(19217..19747)
                     /gene="rppH"
                     /locus_tag="ACH3L8_000594"
     CDS             complement(19217..19747)
                     /gene="rppH"
                     /locus_tag="ACH3L8_000594"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFT4083842.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(20100..20246)
                     /gene="ygdT"
                     /locus_tag="ACH3L8_000595"
     CDS             complement(20100..20246)
                     /gene="ygdT"
                     /locus_tag="ACH3L8_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFT4083843.1"
                     /translation="MLSTESWDNCEKPPLLFSFTALTCDETPVFSGSVLNLVAHSVDK
                     HGIG"
     gene            20432..21121
                     /gene="mutH"
                     /locus_tag="ACH3L8_000596"
     CDS             20432..21121
                     /gene="mutH"
                     /locus_tag="ACH3L8_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFT4083844.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            21190..21903
                     /locus_tag="ACH3L8_000597"
     CDS             21190..21903
                     /locus_tag="ACH3L8_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFT4083845.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            22041..22259
                     /gene="ygdR"
                     /locus_tag="ACH3L8_000598"
     CDS             22041..22259
                     /gene="ygdR"
                     /locus_tag="ACH3L8_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFT4083846.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            22367..23407
                     /locus_tag="ACH3L8_000599"
     CDS             22367..23407
                     /locus_tag="ACH3L8_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFT4083847.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(23440..24630)
                     /gene="lplT"
                     /locus_tag="ACH3L8_000600"
     CDS             complement(23440..24630)
                     /gene="lplT"
                     /locus_tag="ACH3L8_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFT4083848.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(24623..26782)
                     /gene="aas"
                     /locus_tag="ACH3L8_000601"
     CDS             complement(24623..26782)
                     /gene="aas"
                     /locus_tag="ACH3L8_000601"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFT4083849.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            27368..28399
                     /gene="galR"
                     /locus_tag="ACH3L8_000602"
     CDS             27368..28399
                     /gene="galR"
                     /locus_tag="ACH3L8_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFT4083850.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(28406..29668)
                     /gene="lysA"
                     /locus_tag="ACH3L8_000603"
     CDS             complement(28406..29668)
                     /gene="lysA"
                     /locus_tag="ACH3L8_000603"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFT4083851.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            29790..30725
                     /gene="lysR"
                     /locus_tag="ACH3L8_000604"
     CDS             29790..30725
                     /gene="lysR"
                     /locus_tag="ACH3L8_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFT4083852.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(30712..31404)
                     /gene="ygeA"
                     /locus_tag="ACH3L8_000605"
     CDS             complement(30712..31404)
                     /gene="ygeA"
                     /locus_tag="ACH3L8_000605"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFT4083853.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(31533..32951)
                     /gene="araE"
                     /locus_tag="ACH3L8_000606"
     CDS             complement(31533..32951)
                     /gene="araE"
                     /locus_tag="ACH3L8_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFT4083854.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(33266..34027)
                     /gene="kduD"
                     /locus_tag="ACH3L8_000607"
     CDS             complement(33266..34027)
                     /gene="kduD"
                     /locus_tag="ACH3L8_000607"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFT4083855.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(34057..34893)
                     /gene="kduI"
                     /locus_tag="ACH3L8_000608"
     CDS             complement(34057..34893)
                     /gene="kduI"
                     /locus_tag="ACH3L8_000608"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFT4083856.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
BASE COUNT         8599 a         9569 c         8629 g         8174 t
ORIGIN      
        1 aaaccgagct ggtcgaggga ttccgccatt cggttcccta tatcaatacc caccggggaa
       61 aaacgtttgt catcatgctc ggcggtgaag ccattgagca tgagaatttc tccagtatcg
      121 ttaatgatat cgggttgttg cacagcctcg gcatccgtct ggtggtggtc tatggcgcac
      181 gtccgcagat cgacgcaaat ctggctgcgc atcaccacga accgctgtat cacaagaata
      241 tacgtgtgac cgacgccaaa acactggaac tggtgaagca ggctgcggga acattgcaac
      301 tggatattac tgctcgcctg tcaatgagtc tcaataacac gccgctgcag ggcgcgcata
      361 tcaacgtcgt tagtggcaat tttattattg cccagccgct gggcgtcgat gacggcgtgg
      421 attactgcca tagcgggcgt atccggcgga ttgatgaaga cgcgctccat cgtcaactgg
      481 aaagcggtgc aatagtgcta atggggccgg tcgctgtttc agtcactggc gagagcttta
      541 atctgacctc ggaagagatt gccactcaac tagccatcaa actgaaagct gaaaagatga
      601 ttggtttttg ctcttcccag ggcgttacta atgacgacgg tgatattgtc tccgaacttt
      661 tccctaacga agcgcaagca cgggtagaag cccaggaaga gaaaggcgat tacaactccg
      721 gtacggtgcg ctttttgcgt ggcgcagtga aagcctgccg cagcggcgtg cgccgctgtc
      781 atttaatcag ttatcaggaa gatggcgcgc tgttgcaaga gttgttctca cgcgacggta
      841 tcggtacgca gattgtgatg gaaagcgccg agcagattcg tcgcgcaaca atcaacgata
      901 ttggcggtat tctggagttg attcgcccgc tggagcagca aggtattctg gtgcgccgtt
      961 ctcgcgagca gctggagatg gaaatcgaca aattcaccat tattcagcgc gataacacga
     1021 ctattgcctg cgccgcgctc tatccgttcc cggaagagaa gattggggaa atggcctgtg
     1081 tggcagttca cccggattac cgcagttcat cacggggcga ggttctgctg gaacgcattg
     1141 ccgctcaggc gaagcagagc ggcttaagca aattgtttgt gctgaccacg cgcagtattc
     1201 actggttcca ggaacgtgga tttaccccgg tggatattga tttactgccc gagagcaaaa
     1261 agcagttgta caactaccag cgtaaatcca aagtgttgat ggcggattta gggtaagcga
     1321 tgaaaatcac cggatgcggc aggcgtaaca ctcgtgcgtc gcatccggca attacgttta
     1381 ttcccgcgaa ctaaacaacg ccgccagacc actgcgccgc tcagtacgag tggcgattgc
     1441 cgcacttaat atacgctcat cggcatacag cgacagacga cgacgcgcgc gggtcaccgc
     1501 ggtgtatacc agctctcgcg ttactaccgg cgtgcgttgg ctcggtaaaa tcaacgccgc
     1561 atggtcgaac tccgatccct gcgatttatg taccgtcatc gcccacgtag tttcgtgctc
     1621 tggcaagcga ctcggttgca cagacttaat attgccgtcc ggcatcgcaa accagacgcg
     1681 cgtcccctgc ccgcgatcca gcgcaatacc gatatcgcca ttaaacaacc caagcgcgct
     1741 gtcattacgg gcaatcatca ccggtcggcc ttcgtaccaa cgagagtgcg gatgacgatg
     1801 aattttgcgc ttctgttgca taaactgttc aattcgctca ttcagtccag ccacgccaaa
     1861 cggcccttcc cgcagggcgc acaaaagctg gtactcattg aacgcctgaa tgattaaatc
     1921 cggctcggca cgcgcttgca gcagatccag ataacgtccg taacccgcaa gagcttcctc
     1981 aagcatcgca atataatctt cgccgctctg taaaagtcgt ttttcgatat cagtaaaatc
     2041 ctgctgaaaa acggttttca ctgccgtttt atcaccacgg ttgatcgccg cagctaactg
     2101 accaatgcca gaatcgctgc cgaaacgata gcttttttgc agcaggcaga gactgtcgcg
     2161 caaagatgcc gcttctgtgc cggttcctgc cggaacgtga gtccccgtca ggcggcttag
     2221 ctgcctggca cgctcggcgg taaagcccgc gttggcataa gcgcagatat cgcccagcac
     2281 agccccagcc tcaaccgagg ccagttgatc acgatcgccg agaaagatca ctcgcgcatg
     2341 atctggcaag gcgtcgatca gtctcgacat cataggcaga tcgatcattg acgcttcatc
     2401 taccaccagc acatcaagat gcagcgggtt accggcatga tgacgtaaac gctggctacc
     2461 cggctgcgcg cccagcaatc ggtgcaaagt gctggcatct tccggaatgc gtttcttttg
     2521 ttcatcggtc agcggtaact gtcgcaaagc cttgccgaga gattcggtta agcgcgcggc
     2581 agctttaccc gttggtgcag ccagacggat acggcagcgt tcgccgtcgg ccatttgaat
     2641 taacgctgcc agcaacttcg ccacggtggt cgttttaccg gtgcctgggc cgccggaaat
     2701 caccgagatc cgccgcgtca gcgccactgc cgctgcaact ttttgccagt taatttcatc
     2761 gcttactgga aaaagtttgt ccagggtttg cgccagtaga gcttcatcaa cctcaatggt
     2821 atgattcacc tcattgaaga agcgcgctac cgtccgctcg ttacaccaca tgcgattcaa
     2881 ataaagacga tcgccacaga ggatcatcgg cgtgggttca tctcccctgc tgaccgcttg
     2941 agaagccagc aagcattctt cccaattttg tagctcaccg atttcactga cacaggtcgc
     3001 caacagcgga ttcgacgcct cgttattttc cagtcgtgaa agcggcaaac agacgtgtcc
     3061 ctctccggca tcatgactta acagtgccgc cgcgagggtg acggcaggat gttcatctcc
     3121 cgccacggtc agggcaaatt gcacatccag cgggcgtagc tgtttgtgct ccacagcttc
     3181 cagtaattgc ttttgcaatt tcattacgcc tcctccaggg tcataccggc aaacatctca
     3241 tccatcaggg caatcaaccc ggcgttgggt cgggttgtgt aaatcccttg ttgcggatgt
     3301 tctttatcaa cgccacgcag gaacagatag ataacgccgc caaagtgaag atcatagtcg
     3361 taatcagcaa tgcgatggcg cagataacga tgcagcgcca gggtataaag ctgatattgc
     3421 agatcatagc ggtgtgcctg catcgctgct gccatagcct gttgggtgta agccgaactg
     3481 tcttcaccca gccagttgga tttatagtcg agcaggtaat aacgcccttc gtggcggaat
     3541 accaggtcga taaagccttt taacatgccg cgtacctgca tgaactccag cggcgggcag
     3601 ccagcggata gcgggtcaaa ctggcgaatt agtgcatcaa gctggctggc gatgagcggt
     3661 tcgctaatcg gcaggtaaaa ctccatctcc acctgtttat cgcggtcgga aagctgactc
     3721 aggctaacac ccgtttcatt gagaggtgcc tggaggacag ccgtgatcca ctcggtcaac
     3781 accggttccc actgcgattc aaagccgccg agttccagtt tttcctgtac ccagttcggg
     3841 tcaaccggct gggtaaaatc gaggtcttca aacaaactgt gcaagaatgt ccccggtgac
     3901 gcaccgcgcg ggaactgatg cggtgttaac gtcggttctt caacgacgct gaccacgcct
     3961 gcggcatcaa catccagccg tggcatcaga tcctgagcga taccgtgacc acgctgctgc
     4021 aagccggagt agctggtgac acgccagtta tcgccgggca atcgttgtag tgtcctcgca
     4081 ttcagttctg cagttaaagc atcattaacc tgccagggct ggttatcacc agtttgtgcc
     4141 gtttgccagg caatatcatc atcgcataac gcttcaatac aggtgcgaag ccctgccgca
     4201 tcttgcggtt cccctttttg cagcaaacgc ccgagcgcac tttggtggac gtcggtgtca
     4261 ccttttttat cgccacgacg gcgcaccagc ggtgcaacgc cgagactgca atgccaaacc
     4321 gaacgcgtca gcgccacgta aagcaaacgc agatcttccg ccagacgttc gacctccgcg
     4381 aggtcgacgc tttctggcgc agcattaaga tccaaaactg cctcaaacga gtggcgatcg
     4441 tgataaaacg cctgatcctg gacgcggaaa ttggtaataa atggcaacca gaccagcgga
     4501 tattccagcc ctttcgattt atggatcgtg acaatctgca ccagatgttt atcactttcg
     4561 aggcgcattt gttggctgga ggcattactg tctggctcga ggatatgttg cgataaccag
     4621 cgtaccagcg catgttcact ttccagctgc gttccggctt cttgaagcag ttcgctgata
     4681 tgcaagatat cggtaagacg ccgctcaccg cctgccgttg ccagcaagtt ttctgcaatg
     4741 ttacgcgccg acatcagcgc ccgcagcatc ggcataacgc cacgtttgcg ccagatttgc
     4801 cgataaccat cgaactcttc gactaccgca tcccacgcat gttcgtcatt attcagcgtt
     4861 tcaatatcca gcgcattcag ccccatcatt gacgttgcca gcgcactgcg cagggtgttc
     4921 tcacgttcgg gcgtcatcac cgcctgcaac aaccaaagca tttcctgcgc ttccagagtt
     4981 tcaaaaacac tgtcgcggtt cgaaaggtaa acggaaggga tttccagcaa cgttaaggca
     5041 tcgcgcacct gggcggcctc ctggcggctg cgcaccagca cgctgatgtc cgaagcacgc
     5101 accggacgcg cgtcgtcgcc gttcatcagc aacgcttcgc cccgctgtcc ggcttgtagc
     5161 cagtcgcgga tttgcgcagc acatacctgc gccatggtac tttgatagtc gccaacgccg
     5221 cagctttcgc cttccatcag ccacattttc atcgcaggct gcgtttcacc tttaaataca
     5281 aaacgtaacg cctgattttt cccggctgat ttcactggaa taaacggtat ttcgcgaaac
     5341 atgaacgtgt catcagtctg gctgaaaagc ttgttcacac tgttcaccat tccaggtgcg
     5401 gagcgccagt tggtgtctaa agtgtagtgg gcatgaactt cactacgcgc cttcatataa
     5461 gtgaagatat ccgcaccccg gaatgcatat atggcctgct tcgggtcgcc aatcagcaac
     5521 aatgcggttt ccggctgatg gtgccagata cggcgaaaaa ttcggtactg ctgggggtcg
     5581 gtatcctgaa attcatcgat cattgccacc gggaatcgcg tacggatcgc cgctgccaac
     5641 acctcgccgc tttcgctacg cagcgcggaa tcgagccgac ttaacatgtc atcaaaaccc
     5701 aattcgccac ggcggcgttt ttcacgcgct actgtttcgc ggatctcagc caatgcgcgg
     5761 gtgatcaaca gatcgcggat cgacaatggt tctgcaagca gttgatcgat cgcctcaaac
     5821 agtggatgtc gcggggtttc ccccccggcc ttcgtgcgat cttctaagaa acgctgggag
     5881 aatttttcca gcgactccgg caactgataa ctgtttgtct cttcttctgc ccaggcgctg
     5941 atcttttcga tccatttagc ctgattgcta cggttaaact tgcgtcgatc aataccagaa
     6001 gattcgatca gcgcatccag ttcacccact gcgtcgcgcc actgctgttt taccgtatca
     6061 atacgcgcca caatttgcgc gtggcgggaa gccagcgttt catcatcggg cggcggtgct
     6121 ttgataaccg gcggttcgcc ttgcagataa cgattaatat cgcgcagcaa cgcctgcggc
     6181 cctttccagg tttcaaagac gacctgggct atttcacgcg gcagcgggta gcagtggcga
     6241 cgccagaaat cggcgcaggc ctggtagcgt agcagagact catcttcaat cagctgctgc
     6301 tcaaacagca tgccggattc aaaggcattc aggttgagca tgcgctggca aaagccgtga
     6361 atagtaaaga ctgccgcttc atccatctgc cgttcggcta acaacaacca ctgcgcggct
     6421 tgcgctttat cgtcgatctc ttccagcagg cgttcgtaca gtggattgtc ggtggtttca
     6481 cgcagacagg cgatgcgcaa ctcgtggata ttgctacgga tacgaccgcg caattctgcc
     6541 gtggcagcct cggtaaaggt gaccaccagc agttcttcaa cgatcagcgg gcggggaaag
     6601 gcggcggaac cgcctagtcc aagtaacagg cgcaaataga gcgccgcaat cgtaaaggtt
     6661 ttgcctgtgc cggcagaggc ttcaatcagg cgctcgccct gtaagggcaa gcgcaaagga
     6721 tctagtgtct cggcgacatc actcattctt ttcactcatc aggggcattg tttgctgcaa
     6781 cgcgctgacg ttctcccaca ctttccagcc ttcaggatgc acatattcgg ctttcccgtt
     6841 ctggctgccg gaaatctgcg acagaatagc catgccttgc ggctcgacca ccgcctgatg
     6901 gaagaaatca gcaagttttt gcggcgtcag cagttttatc tgggccacga ttttatcacg
     6961 cgaatcgaag cgcatattgc cgcgatcgaa atctttactt aacttcgatg cttcttcgcc
     7021 gagcgtttgc ggtgcctgca gcatctgggt aattaccgcc tgctggattt gcgcaaactc
     7081 atctggcttc atcgctcgca attttgcctc tgcggttggg aaaaacgcct tgtaacgctc
     7141 ccacaagaat gaaggctgtt tatcattgct ttgcaaaagg aagcccatgc cccactgacg
     7201 ccccacgctc attggaaacg caaacacggc atagcccaat tgttcttcgg tacgcaactg
     7261 attgtagaac cacggctgta cgatctgccc caacagagag ctataggctg agctggtgta
     7321 ttcatcgtag ccagtcggta caaataccgc tgccagtgcg gagtcggtgc tgttaccggc
     7381 tttttcaaag atgacggatt gttttttatc gaccactaca tctttgtttc gacaccactc
     7441 tgaaccatca gcgcccaact gtttttgcac atcgcgtgcc agcgttgttg cctgggcctc
     7501 ggtcatgttg ccgataacca taaactctgg tcgagcccct gattttaagg cgtcgcgata
     7561 ggccagcacc tctttcaacg taatggaggg caaaatttta cgccgttcat ctcgcgagaa
     7621 gtacggcact tgcgagagca tctgcgcggg cataatcgcc tgctcaaacg ctttaccctt
     7681 ttctgcggaa tccatcatct ggttatacca ggacttcgcc tgctcaagct gctcttccgt
     7741 agcggtatag ctaaagtacc cctcgagcaa tgcctggaac agctgcggca gacgctgggt
     7801 gtaaccatta gcattaatca taaggccgtt gttagcgttg gtggaaaaac ttatgccacc
     7861 aaccgacgcc tggttgctta actgatcaag cgccagccct gcgagataat cattgagcgc
     7921 aaacatcacc tgattgcggg cgctgtccat ggctttcgga ttacgcaaaa tcaggctgac
     7981 atcagctttg ggctcgctgg caaaataacg gcttggcgca tacaccacgc gcagattcga
     8041 ctcatcaaca atcagctctg gatggtcgta tttcttctct gacttaatca gcgagaaatc
     8101 atcaggaata taagggttaa gctctggcaa agagagcgca atgtcggcgg cttttttctg
     8161 ccagtcggcg aaagtttgtg cgctgatttt atcgacctga tacggcgcat cgacaaagta
     8221 agccgttttg ttgtgcggct ctttcgggct gatataccag atacgcgcat tctgcggcgt
     8281 catcatcgcc agacgttcct ttactgcttt agcatcgtac cgatcggcaa tattgactgc
     8341 atccagcgta tgctcaacag gaacgcgaat catggtatct gccagccatt cgacgtaatc
     8401 catatcacgg gtgatcgacg gataacggaa gtcgatatcc agcacattcg ccagttcatc
     8461 gaagtattgt ttatcaatgc ctttttcacg taacagattg agatagctaa aaattgccgc
     8521 cacaacctga tcgcgattag ccaggccttt atcagttaaa gacgcagaga tcgctaatac
     8581 gccgctgttg ccgttgacga taggatcgga gttggcgcta atgccctcaa ctaatccctg
     8641 cttttgcagc cagtcagaaa gtgtacctgg gctgcgattg ccaatcagat aggtaatcaa
     8701 ttcatcggtt ttactacgga acttcgctga gttgttatcg atgcgaaact caacgcgcaa
     8761 cactttacgc ggcagcgcag ggacgtaatg aatgataatg cccttttgcg cgtcggtgac
     8821 taccggcacg gtgatttccg gttttttgct ctctttgttc ggcacgcgac caaaggtgtc
     8881 cgccgccatt tttgccaact ccggcagcgg tttattactg taaataaccg ccttcatcaa
     8941 attggcggaa tagtacttct cgtggaaatc tttcagcgcc tgctgcaccg gattaccagg
     9001 tttgtcgctt aaagtttcga ggttaccacc agaaaacttt gaaccggggt gtgccgggtt
     9061 aatggtttct gcgctgacct gtgccatgcg catcccgtca cgcgtacgcg ccatggttaa
     9121 ttcagcgttc actgcattac gctcacgttc ggcgtatttc ttgtcgagca agggttctgc
     9181 aatagcatcg gccaggcggt ctaccgcacc gggcaaggcg tcgttctcaa cttccagata
     9241 gaaagccgtg cgatacggcg cagtgctggc attgtgacta ccgccgtgca ttttgagata
     9301 ttcggccaga ctgtcagcct gcgggtactt tttcgacccc atcagactca tatgttcaag
     9361 gtaatgtgcc agcccctggt acgcctcggg atcttccagc gacccaacgg gcactaccag
     9421 cgccgagagc gatttaactg cctgcggatc agaaaccagc aagaccacca taccgttatc
     9481 cagacgtata gcctgatact ggcggttatc tttatcactt ttacggatgg tttcctgaat
     9541 cggctgccat cccgtttctg cctgacttaa tggtgcccag agggcaacaa acaacaataa
     9601 tgctttgaac caggtgctgc ggggcattca cggacctcat aatcaacttt attttctgga
     9661 cagattcaac aggttaacgc aaatcatctg ccagaattta atcttgtgct gcacgagtca
     9721 gcctatgttt atataaccat cagtccgtga ctggtgcgca tcataaagta agcagataga
     9781 ttgcgcaatt tttatacaac actcatgact gattaaagcg aaacagcggt aacaggaaac
     9841 gttgcgactg ttcaacgatg gcctccattg tctctggtgt taattgccgc cagagccgtt
     9901 gataccagat atcatcacct tcgccacgca ccatcatgtt gccttcgtaa gcctgaagga
     9961 atttcgtacg ggctttttgc aacgtggaat cgtcatccag catggcatcg ttttgcgcgt
    10021 cataacaggt ttttagccac gcgccgccac tttcaggtaa caccagcaat ggcgcggaca
    10081 ttccttcacg atacccctca atcagttgtg agaggtaatg caaagcctgt tcggctgcaa
    10141 gcggcggaaa acgccactca ccgtctttgc gtagaaaaag gcgactttcg ccattaccac
    10201 cgctggcaca gtagacaagg tgttccagcc aaagttgcat tccctgcgcc acacttaata
    10261 aagagggacg ccagcgcaac aggccatccg gctgcacctg cggcaaccag ccagttatct
    10321 gcacaccgtt gcaggcgaga tcaatttcca tactctgccc cggctggcga caggcaatga
    10381 ctctgtcggc aagctgctgc atctcctggc actgtgtttc ccagaaaatt tcaccaaaag
    10441 cgccatacgg taaatcccct gccgcccgga agcggcggaa caagcgttcg gcatcatcct
    10501 gctcaaccag tgcattcaat aactgctgat tgatttgata acggctaagt ccttccagaa
    10561 taaatggctc ggtgtctggg atttcgctgt cttcagtatg gaagttcacc tgcaaacgca
    10621 tctggaagaa cgcccgcacc ggatgtgccc agaatcgttg tagcgtttcc agcggcacgg
    10681 tttccggtaa ggtaaacggc agcggctgaa caaattcaga atgtgcttta ccagcctggc
    10741 tggccgcagg tagccattca cgagcatagc tttgtcgttc gcctggctgg tagttttgcg
    10801 gatcaaacgg catccgggta tggaggcaag taagatgcgc ttttaccctt gcctcgcttt
    10861 catcacagtt gagcgcttca tcgcccggta gataatgact ttgcccgatg tagtcgatca
    10921 gttcctgcac cagtaccgac gggaaacgtt cactgttatc ctgaatggaa cgcccgatat
    10981 agctgatata gagtttttgc tgcgcggaaa ttaacgcttc caggaacagg tagcggtcgt
    11041 catcgcgacg gctacggtcg ccacgcttcg gtttctggct catcaggtca aagcccaatg
    11101 gcgcaagctg acgtggataa acgccgtcgt tcattcccag caggcaaacc actttgaacg
    11161 gaattgaacg cattggcatc agagtacaaa tgttaaccgg tccggcgaga aaacgctggc
    11221 tgatacgttc ttgatccaga cgctgtgcca gttcatcacg caatagtgac agcggcaccg
    11281 cgtcgccata ctgcgcacct aaaccttcgg cgataatcgc ctgccattgt tgttcgatca
    11341 gcgtcatcgc cgcttcggtt tccgcatccg gcaggaagaa ggcgttgagc atatcgcgac
    11401 aaaccggcaa ccactcttct agcggacgct cctgtgccag cccgcgacgc cagatgttta
    11461 gctgcattag cagtgaagcc agatgcccca ccagttctgc aattaagccg ctcgattcat
    11521 cataaggtag aaccgattgc cactcgccct gcgcgctctc catcgcgtag cccagcaaca
    11581 tacgcgtcag gccaaatcgc caggtgtgtt gtccggtggc ggggagttcc agctcgcgaa
    11641 cgttgtcgtc atctatcccc caacgaatgc cggattcgtt gacccactgg cgtaaataac
    11701 gcagcccttc ttcggtgatg tcaaaccgcg ccgccagcac cggcacatcc agcagcgcca
    11761 gcacatcctc tgacacaaag cgactgtcag gcagtgataa caggctgata aacgcttcca
    11821 gtaccggatg tgactgccgc gcacgacggt cggaaatggc gtaaggcagg taacgatccg
    11881 caggtgcgct accaaacact gcctgaataa acggactgta gctgtcgata tcagccacca
    11941 tcacgatgat gtcgcgcgga gtaagtgtcg gggcttcctc cagcatcgcc agcaagcgat
    12001 cgtgtaaaac ttcaacttca cgctgcgggc tatggcaaac gtggaatgtg atactgctat
    12061 ccagtggatc aagcgggcgt ttgttatcgc tacgggaaaa ctcttcgatg ttcacacccg
    12121 caacggcgcg gttttccagt tccagaatgt cagactgaat gttatgcagc aggttatctg
    12181 gcgtcacatc gacaaaagcg tccagctcct ggctgctctc caggtcagaa aggagataaa
    12241 tgtagtcgcg cccaagcttc ccccatgagg ccagcagcga gttgccgaca tcctgttcac
    12301 catcgctgtt aaagagctgt ccggcatttt cgctatcgcg aaatagcggc aattcgcgat
    12361 cttcaaaact gtgtcgacgc tgacgagtca gcagtttcgc cagataagca ggatctttaa
    12421 tatcgcccca gtaataacgg caggggttgg taaacaggag atggatttca atatgtttac
    12481 ccagcgcctg gagcgcctgg agataaacag gcggtaacgc ggaaataccg catataaaga
    12541 cgcgcgaagg taaccccggc gggcaggtcg tcgcggactc cagcgtttcg ataaagcgct
    12601 gatagagatt cgcgcggtgc cagcgcggtt gtccgagttc atgggtatat tccaccagcg
    12661 ccttccacaa cggggcttgc caggcctgtg cttctcccag cccttcaacc aaatgtcctg
    12721 tttcccactg tgccagccag tccggacgat agaccagata ctggtcaaac aggtccgccg
    12781 cttttgagga aagctggaac agttttcgct tgtcgctatc gtcagtcaga taatgccgca
    12841 acagggtaaa gtcttcgcgc tccagcaatt gcggcagcag agtcatcagt ttccagctca
    12901 tgctctgttt gttaaaggcg ctctctttgg ggatttccgg taacacccgg acgaacatat
    12961 cccagataaa gctcgctggc agcggaaaat caatgtttgc cgcaatacca aacttttgcg
    13021 acagggtcat ttgcagccac tgcgccatac cggtactttg caccagaatc atctctggtt
    13081 cgaaaggatc gtccagccgt tcgcgttcga caataaactc catcaacgct tccagcacgt
    13141 ccagacgatt ggaatggtag acccttaaca tagcggctcc tgactactga caattcgggc
    13201 aatgcaggcg ggtcatctcg ccttctctgc ccccgggtga aactagcgta acgctgatgc
    13261 tgacacatcc cgcctgcgat gtctgcattc ggttgacctg ccagttggca ggtggcgaaa
    13321 tcgcgcgcag ttgcgtttgc tgccaaccat gccgccagag cttttggtac tggtttctgc
    13381 tggcgagact gttcattgat gttcgctgga tacccgataa cgcagtgaca atcatcacca
    13441 tcagcaccat cagcaccatc agcaccatca gcaccatcgc aaccattacc tccggcaggc
    13501 taaagccttg ttgattcttc agggaagctg acataatgcc ccctctttca gcggacaaaa
    13561 atcgctccag ccgcgtggcg aaaaaacaat ttttccatca atgacttcgc ctgttcgcca
    13621 caacgaaacg ccttcataac cggcaatcaa taaggcttca ttatctgcca gtaaacgcaa
    13681 acaaacctgg gcatcggttc cagcgtactg cgagcactga actgctggct gcgtctgcca
    13741 ggagtgcatt tttccccacg ccagcgccga ctgaacgatg gcctggcggc gcaatgactg
    13801 actttccatg ctcacgcgag aggcaaaact gcgatcctgc tgactcattc cttgcaatag
    13861 caagctaccc aaaaccagca gcatcaggac cagagccagt gacgaaacac ctttttcgcg
    13921 gttcacaggt tgaatcctgt cacgctatag ctggcatcca ccacggtttg cggttcagac
    13981 ttactggcag cacgcatatt aaccgtcaac accggcgaga agccgctgac atcctgacgt
    14041 acgacctgaa aagtgtcgat aatgatggca tccggattag tgactttatc ccagccctta
    14101 ccttcacagg atgtcgcacc gcgtagcgtt tccagcacat gctccttcag acgaaatcca
    14161 atctggtcgg actcttttac cggttcgcga tcccagatac cgttactgtt cgcatcccac
    14221 tgcacaatga tacagtcacc ctgtccgaca atttccagcc cttcgccggt acagctgcca
    14281 tgacaataac ccgccctctg gagatgcttc gcgacggtaa atacccgcag ccagatttca
    14341 tcttccagtg ccagcttacg agtgctcgtt aaactttcac gctgtaacgc aggcagaaag
    14401 cgtgcagccc ccagcaacaa tacgctactg atcgccatag caatcaacac ttccagcaga
    14461 gaaaaacctt gctcttttac aggcatcctt ctgtttctcc ttgctgataa agccggagtc
    14521 ttccccacgg cgaaaccacc agccaccact cgcccgttga gtttttgaag cgaatatgcc
    14581 cggcccatgc ggtattgcgc aggccaaaga aagcaagcga aggtgtcagg tcgctcattt
    14641 cgacttcggg ccagcgtggc acaaagacca atggtgaact gccatgacag gtattggccc
    14701 cagcagcgga actcacaagg caccataacg tcccctccct gataacgctg atactgtggt
    14761 cgcggttatg ccagttggca tcttcacgta aatagagcaa atagtcccgc gcctagctgg
    14821 cggtttgcca aagccgttgc gactgctgcc agtattgcca gccatagagt ccacttgcgc
    14881 ttagcatgac caaaatcagc atcgcgacca gcgtttcaat cagcgtataa ccacgttgtg
    14941 ttttcatgcc ggcagtatgg agcgaggaga aaaaaagacg agggccagtt tctatttctt
    15001 cggcgcatct tccggactat ttacgccgtt gcaggacgtt gcaaaatttc gggaaggcgt
    15061 ctcgaagaat ttaacggagg gtaaaaaaac cgacgcacac tggcgtcggc tctggcagga
    15121 tgtttcgtaa ttagatagcc accggcgctt taacgcccgg atgtggatcg tagccttcaa
    15181 tctcaaagtc ttcgaaacgg tagtcgaaga tggattcggg tttacgtttg ataatcaact
    15241 tcggcagcgg acgcggttcg cggcttaatt gcagatgagt ttgatccata tggttgctgt
    15301 acaggtgcgt atcgccaccg gtccagacaa aatcacccac ttccagatcg cactgctgcg
    15361 ccatcatatg caccagtaac gcgtagctgg caatgttgaa cggcaggccg aggaagacgt
    15421 cacaggagcg ctgataaagc tggcaagaga gtttgccgtc tgccacatag aactggaaga
    15481 atgcatggca cggtgccagc gccattttat ccagttcgcc tacgttccac gctgaaacaa
    15541 taatgcggcg cgaatccggg tcgtttttca gctggttcag taccgtagta atctggtcaa
    15601 tatgacgacc atctggcgtt ggccaggcgc gccactgttt accatacact ggcccgaggt
    15661 cgccattttc atcggcccat tcgtcccaga tggtgacatt gttttcgtgt agataagcaa
    15721 tgttagtatc gccctgcagg aaccacagca gttcgtggat gatggaacgc aggtggcaac
    15781 gtttggttgt caccagcggg aatccatcct gcaggttaaa acgcatctga tgaccaaaaa
    15841 tggaaagcgt tccggttccg gtacggtcgt ttttctgtgt gccttcgtcg agcacttttt
    15901 gcatcagttc taaatactgt ttcatggttc ctcaggaaac gtgttgctgt gggctgcgac
    15961 gatatgccca gaccatcatg atcacacccg cgacaatcat cgggatggaa agaatttgcc
    16021 ccatgctgat gtactgcacc caggcaccgg taaactgcgc gtcgggctgg cggaaaaact
    16081 caacaatgat gcgaaacgcg ccgtagccaa tcaggaacaa gcctgagaca gctcccattg
    16141 ggcgcggttt acgaatatac aggttgagga taataaacag caccacacct tccagcagca
    16201 gttcgtaaag ctgtgatggg tggcgcggca gcacaccgta agtgtcgaaa atggattgcc
    16261 actgcgggtt ggtttgcagc agcaaaatat cttctgtacg ggagcctggg aacagcatgg
    16321 caaacgggaa gttcgggtca acgcggcccc acaattcacc gttaataaag ttgcccagac
    16381 gcccggcacc aagaccaaac ggaatgagtg gtgcgataaa atcagagacc tggaagaagg
    16441 aacgtttagt acggcgggcg aagataatca tcaccacgat aacgccaatc aggccgccgt
    16501 ggaaagacat gccgccgtcc cagacacgga acagatacag cggatcggcc ataaactgcg
    16561 ggaaattgta gaacagaaca taaccaatac gtcccccgag gaagacgccg aggaagcccg
    16621 catagagtaa gttttcaact tcatttttgg tccagccact gcccggacga ttcgcccgac
    16681 gtgttgccag ccacattgca aaaatgaacc ccaccagata catcaggccg taccagtgaa
    16741 gcgccacggg tcctattgag aaaatgaccg gatcaaactc cggaaaatgc agatagctac
    16801 tggtcatctg tcaccacaag ttcttgttat ttcgctgaaa gagaacagcg attgaaatgc
    16861 gcgccgcagg tttcaggcgc tccaaaggtg cgaataatag cacaagggga cctggctggt
    16921 tgccggatac cgttaaaaga tatgtatatg atccgcgcta taaccctccg cgaatcagcc
    16981 cgcccatgcc gcgacgctcc ataaaggctg caacctgatg acgaacttcg gtcgccagtt
    17041 gcgcttccag actacgctgc gcaagatttt ctgcttcggc aaaatcaatg cgccgcagca
    17101 ggtattttac ccgcgctaca gaacgtccgt tcatagacag atggcgatac ccaagcccaa
    17161 tgaggattgc cacgcacatg ggatcgcccg ccatttcacc gcacaaacga agatcgatac
    17221 catgtatttc cgcttcccgg gcgatcatcg ccagagctcg taacattgca ggatgaagac
    17281 tgtcgtaaat gttcgccacc cgggtattgt tgcgatcaac ggccagaatg tattgagtca
    17341 gatcgttggt gccaacagag atgaaatcga ccctctttgc cagatgcggc agcataaata
    17401 ccattgacgg cacttccagc atgatgccga tacgtggttt gggaatttcg taaccgatca
    17461 tctcctcgac ttcacgtccg gcacgttcaa tcaggcggcg tgcttcgtca acttcatcga
    17521 ggctggtgac catcggcaac agaatattca ggttgcccgt agcggcatta gcacgcagca
    17581 tcgcccgcac ctggatcaag aagatctccg gctgatcgag cgtaatgcga atcccacgcc
    17641 aacccaggca tggattctct tcgctgatcg gcatgtaagg cagctgctta tctgctccga
    17701 catccagcgt acgcaaggtg acgggtttat cattgaacat ttgcagcatc ccctgatact
    17761 gcgccacctg ttcttcttcc gacgggaaac cactttgcag catgaatggg atttcagtgc
    17821 gataaagacc gatgccatca atacagctgc ccagtttttc ttcatgttcc gggcttaaac
    17881 cagcattgag catgactttt atacgctcac cgctttttaa ctgcgcgggt aaattgacgt
    17941 catcttccgc cagacggcta agctcaatct cttcactaat tagccgctga tattcttgca
    18001 gcagtaccgg ctccggatcg accagcaatt caccgcgata gccatcaacg atcagcgtcc
    18061 tacgatgcag caccgaaggc tgaatatccg cgcccatcac ggtagggatc cccagcgcac
    18121 gtaccatgat cgcagcatgg gagttggctg cgccatctcg cacgacaaca ccgactaagc
    18181 gatcctgggg cagctcagca agagttgtcg ctgacagttc atctgccacc agaatgaaac
    18241 gttccggcca ggcgttcggc ccttgattag cgtcatcaag atgaaacagc aatcgctgac
    18301 ccagcgcacg taaatcacca gcccgctctt tgagatagtt atcgcttagc gcggcaaact
    18361 gttcggcaaa tttttcaatg accgttttta ccgcccactc tgccaccgag cctttatcaa
    18421 cctcggcaaa caattcgcga cgcagccggg tgtccgaaag caggtgcgag taaagatcga
    18481 aaatagccgc cgtttctttt tgtgctccgg cggcaaagcg tttgctgtag cggcgaaact
    18541 cgtttgccgc ttcttccagc gccccggtca gtcgttcgcg ttccagagcc ggatccagcg
    18601 ttgatgcctg atacacctgt tccattaaag gtaacgtggc atcctgccag ccttcggcaa
    18661 tcgccacacc aggtgctgcc ggtaatgcgc ggattcgcgt ctggcgatat tgcccaaaca
    18721 gggcagtcaa ctgcgactga gaaagaatag ctgccatctg ggtggcaagc gtcaccagga
    18781 aggattcttc actttcgtca tactggcgca actctcgttg ctgtaccacc agtacaccaa
    18841 gcaactggcg acgttgaata attggtacgc ctaaaaacgc gcggaaacgt tcttctttta
    18901 cggaggggat gtatttgaag ctggggtgct tttgcgcatc tgcaaggttt atcggttccg
    18961 ccagcctgcc aaccaggccg acgatccctt catcaaacgc gagcgttaca gtgcgaccgc
    19021 gtggtttttt cagcccccgg gtcgccatca ggtagtaaca acgtcgatca tgatcggcca
    19081 ggtagaccga acagacctcg gtatccatcg caagacagat gtcggtaacc agaatattta
    19141 acgcctcatt caggcgtggt gcgctggcta ccttttcgac tatttcgcgc aggcgagtga
    19201 gcataattgg cgtgacttaa cctcttttac gtcgataagc agatgcgttt tgtggttttg
    19261 gcgtattttc ctgcagtgac atcaccacac tcgcgaactc tttcattacc ctacggtaga
    19321 catcacgttt aaatgacacc acctgtctga ccggatacca gtaacttacc catcgccagc
    19381 cgtcaaactc tggtgtactg ctggtttgca tattgatttc tgcatcgccg cttaccagct
    19441 gcaagagaaa ccatttttgt ttttggccga tacaaaccgg cttcgtgtcc caacgcacca
    19501 aacgtttcgg taatttgtag cgcaaccagt tacgcgttga agcaaggatt cgaacgtctt
    19561 tgcggcttaa tcctacttct tcaaacaatt cacggtacat cgcctgctct gcggattctc
    19621 cggggttgat tccgccttgc ggaaattgcc aggagtgctg accaaatcgc cgggcccaca
    19681 ttacctgccc ctggcgatta caaatcacga tacctacgtt tgggcggtag ccatcgtcat
    19741 caatcaccgg actacctcaa aataaagctt tatatacgaa tgattgtttc atactccagg
    19801 aagacggtaa accactctct gcagggcatt acacactaat aacaattgaa taactcacag
    19861 ttatgtgcag agttataaac agaggaaggg gtggatagcc gtttttattc accttttctg
    19921 tggatagagt tgtgaagaac tacggaatta ctacgggaaa acccggagca ttctgaataa
    19981 gcccgatata gacattttaa aatattcatt attagtcaat gcattacatg taatttgagt
    20041 aatgaaaaag agtaattcgt gacccaggtc acacctctca tttacgggtt ggcgaaagat
    20101 caaccaatgc cgtgtttatc cacagaatgt gccactaagt taagcactga accactaaaa
    20161 actggagttt cgtcgcacgt caaggctgta aatgaaaaca gtagtggagg tttttcacag
    20221 ttatcccagc tttctgtgga taacatggtg taagatcctg tttattttca gtgaccagat
    20281 ttggaaaacc tattgcagtg ttgcgcaact cgattaccgg cagcctaaaa agcaatataa
    20341 atcagagaat tgaacaacac atgtggaaaa agttacactg cgaatattcg gcacataatt
    20401 gctgtttgtt ttttaaccaa ggtatcatga catgtcccaa cctcgcccac tgctctctcc
    20461 tcccgaaact gaagaacagt tgttagcgca agcacagcaa ctttctggtt atacattggg
    20521 agaactggcg gcacttgacg ggctggttac gccagagaat ttaaaacgcg ataagggctg
    20581 gattggcgtg ttactggaga tctggctagg tgccagcgca gggagtaaac ctgagcaaga
    20641 ttttgctgct ctgggcgtgg aacttaaaac tatccctgtg gatagtcttg gtcgtccgct
    20701 ggaaacaaca ttcgtttgtg ttgcccggtt aacgggtaat agcggggtga cctgggaaac
    20761 cagccatgtg cgccacaagc tcaaacgcgt actgtggata ccggttgaag gcgagcgcag
    20821 catcccgctg gcgcagcgtc gcgtaggatc accgttgctg tggagcccga atgaagagga
    20881 agaccgacag ctacgcgaag actgggaaga attaatggat atgattgttc tcggtcaggt
    20941 tgagcggatc accgctcgtc acggggaata tttacagata agaccgaaag cagcgaatgc
    21001 gaaagcgctt accgaagcca ttggtgcccg gggcgaacgg attctgacgc tgccacgcgg
    21061 cttttatttg aagaagaatt tcaccagtgc actactggcc cgtcattttc tgatccagta
    21121 gccatcgctt tgacctgccg ctttccgggc atataattac cgcttcattt ttttggcagg
    21181 gctttttaga tgttatttgc atggataacc gatcctaacg cttggcttgc gctcggtacg
    21241 ctgacgctgc tggagatcgt tcttgggatc gacaatatta ttttcctttc tctggtggtg
    21301 gcaaagcttc ccacagcaca acgtgctcat gcgcgccgtc tggggttggc gggagccatg
    21361 gttatgcgtc tggcgctgct ggcatcaatc tcttgggtta cgcgcctgac aaatccgctt
    21421 tttacaatat tcagtcagga aatttctgcc cgtgatttga ttctgcttct gggtggcttg
    21481 ttccttatct ggaaagccag caaggaaatc cacgaatcca ttgaaggtga agaagaaggg
    21541 ctgaaaacac gcgtttcatc attcctcggc gctatcgtgc agattatgct gctggatatt
    21601 atcttcagcc tcgactcggt gattaccgct gtgggtctgt cagatcacct gtttattatg
    21661 atggcagccg tggtgattgc cgtaggcgtg atgatgttcg ccgcgcgctc gattggtgat
    21721 tttgtcgaac gccatccttc ggtaaaaatg ctggcgctct ctttcctgat tctggtgggc
    21781 tttaccctga ttctggaaag tttcgacatc cacgtaccga aaggttacat ctacttcgcg
    21841 atgttcttct ctattgcggt tgaaagcctc aacttgattc gcaacaaaaa gaatccgctc
    21901 tgatactccg tacgctctcc tgcgggaggg cgtactcact ccttttaccc tcacagttta
    21961 agattttcct gctttcaaga tatataacgt cggtttataa acagactatt atcataggta
    22021 ggccaaatga ggataaccgg atgaaaaaat gggcagtaat aatttctgca gtcggactgg
    22081 cgtttgctgt ttccgggtgt tccagtgatt acgtcatggc gaccaaagat ggccgtatga
    22141 ttttgaccga tggaaaacct gaaattgatg atgataccgg gctggtgagt tatcacgatc
    22201 agcaaggtaa cgcgatgcaa attaaccgtg atgatgtttc gcaaattatt gaacgttaac
    22261 aaataaggtc agcatccggc tggccttaag atttttctct ttcccttttc ccttccctct
    22321 gccattttta tattccttat gtcgtgatta taaaaaggaa acggctatgc aatatcaccg
    22381 tataccccac agttcgctgg aagtcagcac gctggggctt ggcacgatga cgtttggtga
    22441 acagaacagc gaagccgacg cccacgcaca actcgactat gccgtcgctc agggcattaa
    22501 ccttatcgac gttgccgaaa tgtacccagt acctccgcgc cccgaaacgc aagggttaac
    22561 cgaaacctac gtcggcaact ggctggcgaa acatggcagc cgcgaaaagt taattatcgc
    22621 ctccaaagtg agcggaccgt cgcgcaataa tgacaagggc atccgcccgg atcaggcgct
    22681 ggatcggaag aatatccgcg aagcgctgca tgacagcctc aagcgcctac agactgatta
    22741 cctcgatctt tatcaggtgc actggccgca gcgcccgacc aactgcttcg gcaaactcgg
    22801 ttatagctgg acggattctg cgcctgcggt ttcgctgctg gatacgctgg acgcactggc
    22861 agagtaccaa cgcgcgggaa aaattcgtta tatcggcgtg tcgaacgaaa ctgcatttgg
    22921 cgtaatgcgc tacctgcatc tggcggacaa acacgatctg ccgcgtattg tcaccattca
    22981 gaacccttac agtctgttaa accgcagttt tgaagtaggt ctggcagaag tcagccagta
    23041 tgaaggggtc gaactgctgg cctattcgtg cctgggtttc ggcacgctga ccgggaaata
    23101 tctcaatggt gcaaaacccg ctggcgcacg taatacgctc tttagtcggt tcacccgcta
    23161 tagcggtgag caaacgcaaa aagccgtcgc ggcgtatgtt gatatcgcca gacgtcatgg
    23221 ccttgatcct gctcagatgg cgctcgcgtt tgtacgccgt caaccgtttg ttgccagcac
    23281 tctgctgggc gcaaccacga tggatcagct gaaaactaac atcgaaagtt tgcatctgga
    23341 gttaagcgaa gacgtattag ctgaaattga agcggtacat caggtttata cttatcctgc
    23401 accataaaga ttaacgccgg ttaaaaccgg cgttaaatat taatgacggc gctgccagat
    23461 ccacagcgcc gttattgcca gcgcaaatag cgcaccgaag ccaatgccaa tgggcacgac
    23521 cgggatgcct accattaccg ccagcgagta aatgcccagc atcaacaaca tggcgctgtt
    23581 ttcgccaagg ttttgtactg caatcgcatt ccccgccccg acgctttttt taccccgctc
    23641 ctgtagcaac gcattgagcg gaacgacaaa aaagcccccc agcacgccaa tcagcatcaa
    23701 caaggcatag gctggcagca gctcgtgttg cagggagaaa atcggtacca ccacgccaat
    23761 caaaatcccg gctggcatac agcgtgacac ggtttccagc gtaaccagct tcgctgccgc
    23821 acctgcgcca accacgatac caatcgctac catcgcgttg agataggtcg gcgtagcgtt
    23881 atcggtaatg cccagcgcca ccggtaccca cagcaccaac aggaaacgca gcgtgacacc
    23941 cgctccccag aataaactgg tgcccaccag cgaaaaacgc gtttcaccat tgcaccatag
    24001 cgaggtgcag gcattcagga aactgcgggt catgttgatg agattccagg actgccccgg
    24061 acgcgccgcc agtttgggaa tgtagatatt ggcaacgacc gcaccaccgt aggccagtgc
    24121 gcatgcggcc agagcgacca ggacatgcca gtcagccagc acaccaccgg ctacggaacc
    24181 gagtaaaatc gccgctatgg cagaagcttc cattaaaccg ttagctttta ctaacttact
    24241 acccgtggtt aattcgccga gaataccgta tttcgccggt gaataggctg cagcaccaac
    24301 acccaccagc gtatagccga gaaacggatt gataccaaag cagatactgg ctgcgcccag
    24361 cagcttcagg ccgttggcaa acatcatcac ccggcctttg gcgaagctat ccgccacctg
    24421 cccgacaaac ggcgccaaaa gaatgtaagc acctacaaac accatttgca ggatgggctg
    24481 gctccactcc ggatagaact gcgctttcag taacgccaga gtggcaaaca gtagcgcgtt
    24541 atcgccaaac gcagagagaa actgcgccac gataaccgct ttcatcccct tcgaccacaa
    24601 cgaagtgtta gtgtgcactg actcactcat cgtgttgttc cgcttcgtct acccagcttt
    24661 tcaacgtgac aaagtcaggt ttgccgctgc caagtaatgg catctgtttc agatagcgaa
    24721 tatcgcgcgg tacagcaagc tccggcacgc cgtgctcgcg ggcatactgt tgcaacttat
    24781 cgcgcgtcag ttcgttatct gtggtgaaaa gcaccagtgc ctcgcctttg ctggcatcgc
    24841 tcttaatcgc agtggcatgg actttatctg gcgaaacacc aagtgccagt tgttccacca
    24901 tttccagcga caccatttcg cctgcaattt tggcaaagcg ttttgcgcgg ccctgaatct
    24961 gcacaaagcc ctgctcgtca aaacgcacaa tatcgccagt gtcataccag ccgcgctcca
    25021 tttcgccgcg aacattctcg gcggtgggca cttccagtac acctggcttc tccacccgca
    25081 gatagccgtt cattatgttc ggccctttca gttgcaggcg tccgccctct tcgataccag
    25141 ggaccgacaa caggcgtgca tccattcctg gtagaatacg ccctaccgta ccgggtttcg
    25201 ccgccatcgg tacgttgata gaaacgacag gcgcgcattc ggtcacaccg tagccttcaa
    25261 ggatgcgcag gccaaattta tcctgccaaa gctgtttggt actttcttgt agtttttctg
    25321 cgcctgccac cacatagcgt agacgataga agtcatacgg gttggcgaaa cgcgcgtagt
    25381 gaccgaggaa agtcgaggta ccgaacaaca cggtgcaact gcggtcatac accaagtccg
    25441 gcacaatgcg gtaatgcagc gggcttggat aaaggaacac ttctgcacct gtaagcagtg
    25501 gcgtaaacag gcctaccgtc agcccaaagg agtgaaacag cggtaatgcc gacataaagc
    25561 gatcgttggt ggtgaagtcg gcaatcgttt taatctgctc gacgttcgcc agaatgcttt
    25621 tatggctatg gacgacgcct tttggatggc cttcagaacc ggaggtaaag aggatcagcg
    25681 cctcttcttc cggctgctgt tttacctgcg ccaggcgcgg catcagcaaa tgagcgaaga
    25741 tccatacttt gtcggcagtg gtgacatctg cttttaaatc ttccagatag acccagcgca
    25801 cctgagtaag ttgctccggc agatgccaga gtttgccttt atcgagaaac tggcgggaag
    25861 tgaagatggt tttgatttca gccgccgtaa tagcactggt cagccctttt accccggcag
    25921 tgtagttcat cattgcggga atgcggcgac gcgcgatggc cccaaaaatc actgccgcac
    25981 tgatgcctgc attgggcagc attaagccga tgcgttcgcc ttcaacactg tatttttcaa
    26041 ggatgcgtcc aacaaacagc gttttcgtaa gcaatttgcg ataggagtct ggggtaaagt
    26101 tgacgtcttc gacacatttc ttcccggctc cgaagcggta cattgcactc agtaaagatt
    26161 cgtacagagt ttcacgcggg cgcaccgcca tacgcgcttc catcattatt tgatgcagca
    26221 tttcgccagc gattttgcga cggtcacggg cacgcggcgc atccggcatc gccacctgcg
    26281 ttggaggcaa aatatgcaga gtaatttgcg ggaacaagcg acgtttaacc agacctttca
    26341 ggcggctgaa gtgcgtaagt tccgcccctt caatacgcac aggaataacc gttgcaccag
    26401 acttcgccgc gacaaaaccc gcgccatcgt agattttcat cagcgagcct gtagtggtga
    26461 tgcgtccttc agggaaaatc accactggtc ggccctgttc caccagacgt accagatgtt
    26521 taatagccat aggttgcgtc gggtcgagag gaacaaagtc gataaatgat ttcagccaac
    26581 gcatatacca ctgttggctt attgaggtgt aaacggcaaa cactggacgc acaggtaaaa
    26641 acagtccaag caaaatgcca tcaataaaag agacgtgatt aggcgtaatt agtacgcgct
    26701 cgcccttcag tgcctgggtg tcacccgtaa cgcgaacgcg atacaaaaca cggcacaaat
    26761 ttcgaaaaaa gctaaaaagc attccaactc cctttgctct gattcagtaa aagcgaatgg
    26821 agggagatta cacgagataa agaacgcgag cgacagtaaa ttaggtgcga aaaaaaacct
    26881 gcgcatccgc gcaggttggt gcaagagaca gggtacgaag agcgtaccga ataatctcac
    26941 caatcaatac ctctgggatc ttgattgtgg tctgcacgac gactcttcgc cagcgagaaa
    27001 acgcaaagga atgaagggaa atgcaacgag gtgtgtaaat tgtcggttac tgttacagat
    27061 tgatgaccgg caaaaaaaaa cctgcgcatc tgcgcaggct ggtgtaattc atgtgctcaa
    27121 cacgaagttg acttcaccta tcaatacctc tgggatcacc actttagcaa cctgaagcca
    27181 aacgccacca gcggtcaatc gcaacagcgt ttcgcaaagt gtaaccaaag gtttgcaatc
    27241 tctttttttg tcttacttat ttgcgtttgg ctcacattcc cacgatgaaa acacgccacc
    27301 ccttgaacca acgggcgttt tccgtaacac tgaaacaatg taagcgttta cccactaagg
    27361 tattttcatg gcgaccataa aggatgtagc ccgactggca ggcgtttcag tcgccaccgt
    27421 ttcccgcgtc attaataatt cacccaaagc cagcgaagct tcccggctgg ctgtgcatag
    27481 tgcaatggag tctcttagct atcacccgaa cgccaacgcc cgtgcgctgg cgcagcagac
    27541 cactgaaacg gtcggtctga tcgttggtga tgtttccgat ccgtttttcg gtgcaatggt
    27601 gaaagcggtc gaacaggtgg cttatcacac cggtaatttc ttattgattg gcaacggtta
    27661 ccacaacgaa caaaaagagc gtcaggctat tgagcaactg atccgccatc gctgtgctgc
    27721 gttggtcgtc catgccaaaa tgatcccaga tgccgattta gcctcattaa tgaaacaaat
    27781 gcccggtatg gtgctgatca accgtatcct gcctggcttt gaaaaccgtt gtattgctct
    27841 tgacgatcgt tacggtgcct ggctggcaac gcgtcattta attcagcaag gtcatacccg
    27901 cattggttat ctgtgctcta accactctat ttctgacgcc gaagatcgtc tgcaagggta
    27961 ttacgatgcc cttgctgaaa gtggtattcc ggccaatgat cggctggtga catttggcga
    28021 accagacgaa agcggcggcg aacaggcaat gaccgagctt ttgggacgag gaagaaattt
    28081 cactgcggta gcctgttata acgattcaat ggcggcgggc gcgatgggcg ttctcaatga
    28141 taatggtatt gatgtaccgg gtgagatttc gttaattggc tttgatgatg tgctggtgtc
    28201 acgctatgtg cgtccgcgcc tgaccaccgt gcgttaccca atcgtaacga tggcgaccca
    28261 ggctgccgaa ctggctttgg cactggcgga taatcgccct ctcccggaaa ttactaatgt
    28321 atttagtccg acgctggtac gtcgtcattc agtgtcaact ccgtcgctgg aggcaagtca
    28381 tcatgcaacc agcgactaac cgcagttaaa gcaattccag cgccagtaat tcttcgatgg
    28441 tctggcgacg gcgaatcaac cgcgcctgac cattatcaaa cagaacttct ggtaacagcg
    28501 gacggctatt gtagttggat gacattgatg cgccatatgc ccctgtatca tgcagtacca
    28561 gataatcacc tgccttcact tccggcaagg cgcgggtttc aacatttccc ccttcctgct
    28621 gggtaaagac atcgcccgat tcacataacg gcccggcgac gacggtttcc accgttggcg
    28681 cgtgttccag agaacgacca tcagctgcca gggcactgat atggtggtaa ctaccgtaca
    28741 ttgccgggcg catcagatcg ttgaacccgg catcaaccag cacaaagtgg cggctaccca
    28801 tttgtttgac gctccgcacc tgagtaatta atacgccaga ctgcgctacc aggaagcgac
    28861 ccggttcaat ttccagtttc acagggtggc ccaaatggcg agcgatttgc tcacgcgcgg
    28921 cattccacag accataataa tgttcggtat caaccgcctc ttcaccctgt tgataaggaa
    28981 tagaaagccc accgcccgca gaaatagcct gtaaatcctg accgaattcg ataacctgac
    29041 gcaccatagc accacacacc tgctccagat gggcataatc aacgccagaa ccaatgtgca
    29101 tgtgaatgcc gactagctgc agatgatgac gttgtatcac gtccagtgcg gcgggcagat
    29161 cggtgtacca gataccgtgc ttgctgtttt cgccaccggt attggttttt tggctatgtc
    29221 cgtgaccaaa ccccggatta acgcgcagcc atacccgatg ccctggcgaa acctggccca
    29281 gttggtcgag cgtatcaaca gaacccgcat tcaccggaat ttgcaattca ctgacgcgtt
    29341 caagcgtcgc ctgatcgata acatctgccg taaaaacaat atcatcgggg tgcgtttgcg
    29401 gattgtaacc cgccgccaac gcacgctcta tttcgcctaa cgagacggaa tccactttca
    29461 cgccctgctc acgcattaag cgcaaaatat gaatattgga acaggctttc tgtgcaaagc
    29521 gcaccacttc aaactgtttc agcgctgcaa tctgccgacg aataatttgc gcatcgtaga
    29581 cccacaccgg gcagccaaat tcagcgggca aacgcagcag attttcggcg gtgagatcgg
    29641 tatcggtgct gaacagtgaa tgtggcataa caaactccag atgagtgctt ttttatgatt
    29701 acgccacatc ataaaaagaa taaaaaatat cgatttatgt cgagtctatg caaaaatgat
    29761 atgaattacc ggattgcgag agagcgctaa tggccgccgt taacttacgt catattgaaa
    29821 tttttcatgc ggtaatgacc gccggaagcc tgactgaggc agcacacctg ctacacacct
    29881 cacagccaac cgtcagccgc gaactggcgc gctttgagaa ggtgatcggg ctgaaattgt
    29941 ttgagcgcgt acgtggacga ttacatccta ccgtgcaagg actgcgtctg tttgaagaag
    30001 tgcaacgatc ctggtacgga ctggatcgca ttgtcagcgc cgcagaaagt ctgcgcgagt
    30061 ttcgccaggg agaactgtct attgcctgcc tgccggtctt ttcgcaatct tttttaccgc
    30121 agctcctgca accctttctg gcacgttatc ccgatgtcag cttaaatatc gtgccccagg
    30181 aatcaccgct acttgaagag tggctctcgg cccagcgtca tgatttagga ctcactgaga
    30241 cactccatac gcctacggga acagaacgta ccgaattact ctctttagat gaagtgtgtg
    30301 tgttacctcc gggtcatccg ctggcggtaa aaaaggtatt aacgccggat gattttcagg
    30361 gtgagaatta catcagcctt tcccgtactg acagctatcg ccagttgctg gatcagctat
    30421 ttactgaaca tcaggttaaa cgacgcatga tcgtagaaac ccacagcgcc gcgtcagtct
    30481 gcgcaatggt acgggcgggg gtaggtattt cggtggttaa cccgctcacc gcactagatt
    30541 atgcggcaag cggtttagtg gtgcggcggt tcagtattgc ggttccgttc accgtcagcc
    30601 tgatccgccc cctgcaccgc ccgtcatcag cgctggttca ggcgtttagt gggcatttac
    30661 aagcggggtt accgaaactg gtcacttctc ttgacgctat tttgtcgtca gctacgacag
    30721 cataaaagcg acagcatcct cggcatggat cgccgcggta tcaaacacag gcaaaacact
    30781 gcgctcttct ggcaccagta aaccaatttc tgtgcagcca aaaatgacgc cctgtgcgcc
    30841 ctgttctgca aggcgagcaa tcacttgcgc ataataagcg cgtgacgctt cggtaaattg
    30901 ccccagacac agttcttcaa aaataatctg attaattttc gcccgttcat ccgcttcagg
    30961 aataagacaa ttgatggaaa attgttccgt cagccgcccg cgataaaaat cctgttccat
    31021 ggtgtaacgc gtacccagca gcgccacacg agtcattccg gccccggtaa ttgcacgtcc
    31081 ggtggcatcc gcaatgtgta agaaaggcag agtgcaacgt gactcaatgg catccgccac
    31141 tttatgcatc gtattggtac ataacacaat accttctgcg cccgcccgct gtaagccaag
    31201 cgccgcctca gccagaatgt ccccggtttt atcccattcc ccgcgacgct ggcactcttc
    31261 tatttcatga aaatcgacgc tatgtagcag catttgcgca gagtgaagcc caccaagccg
    31321 ctgtttaatg ccttcattta tcagacgata gtaaggaatg gtggattccc agctcattcc
    31381 tcccagcaaa ccaattgttt tcattcctct ctcctgatgt gtgttaccgc aacaaggctg
    31441 aataaggaac gagacaaacg cctcaacggc caggtgccca atctctatta acgaaaaaag
    31501 ggccggatgt acagcacatc cggcccgtga aatcagacgc cgatatttct caacttctcg
    31561 cctgccatca gtttgcgttc gatatgttcc agcgtgacat ttttggtttc cggaatgagc
    31621 cagaaagtaa tacccacaaa cgcaatgttc agcgcagtgt agagccagaa cgtaccggca
    31681 gcgccaatgc tatcaagcag tgtcaggaag gccgcgccga taatcatatt cgacacccag
    31741 tttgtcgtcg tcgaacaggt aataccgaaa tcgcggcatt tcagcggctg aatttcagag
    31801 cacaggatcc acaccactgg cgcggcgctc atcgcataac cggcaataca catcatcgtc
    31861 atgccaacag agagccagga caagccactg gaagccgtac cgttatcaaa ctgcatcagg
    31921 caatagccca gcaccagagt gcctaacgcc atcacgctga aaccaatttt cagagccggt
    31981 ttacgacctg ctttatctac cgtaaagacc gcaatgaagg tcgcgaacat aaaggtcagt
    32041 cccacgacca gagtcgcaat catctgttgt tctgtggtcg taaagcccgc cattttgaag
    32101 atacgcggcg cgtagtacat gatgatgttc ataccggtaa actgctgcat cgcctgcaac
    32161 aacataccga gaaacacagc acgacggacg ttacggttga tcttaaacag tgcccaaccg
    32221 ccctgtttta acttcaggct ttcacgaatt tcgttgagtt cttctcgcgc tttttccgac
    32281 gtatcgcgca gcatacgcaa cacttcttcc gcctcaatat gacgcccctt ttccgccagc
    32341 cagcgcgggc tatttggcag gaagaccacc agaataatca gcagaactgc tggtaaagca
    32401 agaaccccca acattgcgcg ccagttaccg ctataactga acgctgtatc ggataaaaac
    32461 gccagcacga tgccgagtgt gaccatcaac tggtacatac tgatcatctt accgcgaacg
    32521 ttttcacttg ccatttcaga aagatacaga ggagcggtgt aagacgcgat cccgacagca
    32581 atgcccagca ccacacgagc ggcgattaac atctctacgc tggtcgcaaa agcggaccct
    32641 atagaaccga gtacaaacag gatggccccc gccatcaggc tgtatttacg ccccaggcgg
    32701 aacgacagcc aaccattaaa cagcgcacca attgctgcgc cgagcatcat gctactaacc
    32761 acccattcct gcaaacgact ggtcagcaca aagtgatcgg taatgaacgg caacgctccg
    32821 gcgattacgc cgatatcaag accaaataac aatcctgcga ccgcagcagc taccgaaaca
    32881 aacatattca tacgccgcgt atcacgcaaa gaacgtggcg ttaaagcaga ttccgtattg
    32941 atagtaacca ttttttcctg ccagcagaga gtaagacatt gtgaaaaaat acgtgaacaa
    33001 ttcacacagg tgtcaggtcg gaaacagcat aaatatggat taaattgctg cgacatgccg
    33061 ttatgtgatg gatattccaa ttttaaaatt aagttgaatt attgagatta ttattaacct
    33121 cctaatttta aagcagataa aattcataaa gttcattaat tgataattga tatggattat
    33181 ttcataacca tgatatggat tatgatgatc tagagatata aaaaaccctg ccatgcggca
    33241 gggtcataaa agtaagagga atgaattaac gcgccagcca accgccatcc acagcaatgg
    33301 tataaccatt tacataatct gaagcgctgg aggcaagaaa cactaccggc cccatcaggt
    33361 cactcggcag tccccaacgg ccagctggaa tgcgatcgag aatttccgcg ctacgttgtt
    33421 catctgcccg cagttgttgg gtattgttgg tcgccatgta acccggagct atcgcattaa
    33481 cattaatgtt gtgtttagcc cattcgtttg ccatcaatcg cgtcacaccc atcacgccgc
    33541 tttttgatgc ggtataagaa ggcacacgga tcccgccctg gaaggagagc attgacgcga
    33601 tattgataat cttgccgcca ttgccttgcg cgataaagtg tttcgccgct gcctgagaca
    33661 tgaagaatac gctcttgata ttcaggttca tgacatcgtc ccagtctttt tcgctgaact
    33721 cgagagcgtc ttcgcggcga atcaatccgg cgttattcac caggatatca atatgaccaa
    33781 attcagctac cgcgcgatcc agcagtccag gaatgccatc aatctttcgc agatcggcgg
    33841 tcaggcttaa aaaacgacgc cccagcgccg tgacctgctc gatggtttca gtcggttcaa
    33901 cgatgttaat gccaacaatg tcacagcccg cttgcgccag ccccaacgcc atcccctggc
    33961 ccagcccagt atcacaacca gtgacgaccg caactttacc ttggagagaa aatgcactta
    34021 aaatcataac aacaccttat tcgtttatgc ccacaactag cgcaaatctt taacgcccac
    34081 atggtccata tcattaaaga cctggttttc accgaccatg ccccagataa aggtataagc
    34141 tttggttccg acgccggaat ggatcgacca gctcggggag atcaccgcct gctcgttatg
    34201 catcacaatg tgacgcgttt cttgcggctg ccccatcatg tggaaaacgc aggcgtcatc
    34261 atccatattg aaatagaaat aaacttccat ccggcgctcg tgggtgtgac acggcatggt
    34321 gttccacaag ttacctggag ccagctccgt cagccccata ctcaattgac aggtttccag
    34381 tacatccggg acaaaatatt tgttaatcgt gcgacggtta ctggtgaggt tatcgcctaa
    34441 cgtgactgga gatacttcgt ccggtgtgac ttttttggtg ggatacgtcg tatgcgcggg
    34501 tgcgcaattg taataaaact tcgccggagt ggcggtatca atactggcaa agacaacttc
    34561 ttttgcacct ttaccaacat acagggcgtc gcggtgaccg atttcatagc attgaccatc
    34621 gacagtaatc gtaccggcac cgccaatatt gataacacct aactcgcgac gttcaaggaa
    34681 atagcttacg ccgagttgtt taccaacttc cccgccaacg gaaaccgttt tagttatcgg
    34741 cataatgccg ccaacaataa ttcggtcaat gtggctgtaa accatggtgt actcatcggc
    34801 gacaaatacc ttttcaacca aaaattcatt gcgcagccct tgggtatcca gcgtttttgc
    34861 gtgcgcaccg tggatgctct gtcttacgtc cacatcaacc tccgataatc agtgaagcgt
    34921 gaaaatagaa aacgatagtg aaacactgtt tcgttttctc tataaaggga t
//
LOCUS       DAZXUK010000015        34051 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZXUK010000015 DAZXUK010000000
VERSION     DAZXUK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34051
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.015"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1342)
                     /gene="mnmE"
                     /locus_tag="ACH3L8_000609"
     CDS             complement(<1..1342)
                     /gene="mnmE"
                     /locus_tag="ACH3L8_000609"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFT4083857.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIF"
     gene            complement(1448..3094)
                     /gene="yidC"
                     /locus_tag="ACH3L8_000610"
     CDS             complement(1448..3094)
                     /gene="yidC"
                     /locus_tag="ACH3L8_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFT4083858.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3097..3354)
                     /gene="yidD"
                     /locus_tag="ACH3L8_000611"
     CDS             complement(3097..3354)
                     /gene="yidD"
                     /locus_tag="ACH3L8_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFT4083859.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3318..3677)
                     /gene="rnpA"
                     /locus_tag="ACH3L8_000612"
     CDS             complement(3318..3677)
                     /gene="rnpA"
                     /locus_tag="ACH3L8_000612"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFT4083860.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3694..3834)
                     /gene="rpmH"
                     /locus_tag="ACH3L8_000613"
     CDS             complement(3694..3834)
                     /gene="rpmH"
                     /locus_tag="ACH3L8_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFT4083861.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4064..4144)
                     /locus_tag="ACH3L8_000614"
     CDS             complement(4064..4144)
                     /locus_tag="ACH3L8_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4083862.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4441..5844
                     /gene="dnaA"
                     /locus_tag="ACH3L8_000615"
     CDS             4441..5844
                     /gene="dnaA"
                     /locus_tag="ACH3L8_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFT4083863.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5849..6949
                     /gene="dnaN"
                     /locus_tag="ACH3L8_000616"
     CDS             5849..6949
                     /gene="dnaN"
                     /locus_tag="ACH3L8_000616"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFT4083864.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            6949..8022
                     /gene="recF"
                     /locus_tag="ACH3L8_000617"
     CDS             6949..8022
                     /gene="recF"
                     /locus_tag="ACH3L8_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFT4083865.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8051..10465
                     /gene="gyrB"
                     /locus_tag="ACH3L8_000618"
     CDS             8051..10465
                     /gene="gyrB"
                     /locus_tag="ACH3L8_000618"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFT4083866.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10705..11103
                     /locus_tag="ACH3L8_000619"
     CDS             10705..11103
                     /locus_tag="ACH3L8_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFT4083867.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            11217..12029
                     /gene="yidA"
                     /locus_tag="ACH3L8_000620"
     CDS             11217..12029
                     /gene="yidA"
                     /locus_tag="ACH3L8_000620"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFT4083868.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12075..12731)
                     /gene="yidX"
                     /locus_tag="ACH3L8_000621"
     CDS             complement(12075..12731)
                     /gene="yidX"
                     /locus_tag="ACH3L8_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFT4083869.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13009..13698
                     /gene="dgoR"
                     /locus_tag="ACH3L8_000622"
     CDS             13009..13698
                     /gene="dgoR"
                     /locus_tag="ACH3L8_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFT4083870.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13695..14573
                     /gene="dgoK"
                     /locus_tag="ACH3L8_000623"
     CDS             13695..14573
                     /gene="dgoK"
                     /locus_tag="ACH3L8_000623"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFT4083871.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14557..15174
                     /gene="dgoA"
                     /locus_tag="ACH3L8_000624"
     CDS             14557..15174
                     /gene="dgoA"
                     /locus_tag="ACH3L8_000624"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFT4083872.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15171..16319
                     /gene="dgoD"
                     /locus_tag="ACH3L8_000625"
                     /pseudo
     CDS             15171..16319
                     /gene="dgoD"
                     /locus_tag="ACH3L8_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            16394..17731
                     /locus_tag="ACH3L8_000626"
     CDS             16394..17731
                     /locus_tag="ACH3L8_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4083873.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALCFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17692..18792)
                     /gene="cbrA"
                     /locus_tag="ACH3L8_000627"
     CDS             complement(17692..18792)
                     /gene="cbrA"
                     /locus_tag="ACH3L8_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFT4083874.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            18893..20131
                     /locus_tag="ACH3L8_000628"
     CDS             18893..20131
                     /locus_tag="ACH3L8_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFT4083875.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTNTWTPDSQWLVFDVRPSGAS
                     FTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFH
                     HRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLD
                     PALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGW
                     VGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVV
                     QRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKL
                     THNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSP
                     DGQWLAWMEGGQLWITETDR"
     gene            complement(20133..20465)
                     /locus_tag="ACH3L8_000629"
     CDS             complement(20133..20465)
                     /locus_tag="ACH3L8_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFT4083876.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20704..20776
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20770..21183
                     /gene="ibpA"
                     /locus_tag="ACH3L8_000630"
     CDS             20770..21183
                     /gene="ibpA"
                     /locus_tag="ACH3L8_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFT4083877.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21295..21723
                     /gene="ibpB"
                     /locus_tag="ACH3L8_000631"
     CDS             21295..21723
                     /gene="ibpB"
                     /locus_tag="ACH3L8_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFT4083878.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            21919..23580
                     /locus_tag="ACH3L8_000632"
     CDS             21919..23580
                     /locus_tag="ACH3L8_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFT4083879.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23619..24293)
                     /locus_tag="ACH3L8_000633"
     CDS             complement(23619..24293)
                     /locus_tag="ACH3L8_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFT4083880.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            24588..26204
                     /locus_tag="ACH3L8_000634"
     CDS             24588..26204
                     /locus_tag="ACH3L8_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFT4083881.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFLEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26204..27018
                     /locus_tag="ACH3L8_000635"
                     /pseudo
     CDS             26204..27018
                     /locus_tag="ACH3L8_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27015..27908)
                     /locus_tag="ACH3L8_000636"
     CDS             complement(27015..27908)
                     /locus_tag="ACH3L8_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFT4083882.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            28075..29790
                     /locus_tag="ACH3L8_000637"
     CDS             28075..29790
                     /locus_tag="ACH3L8_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFT4083883.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            29825..31267
                     /locus_tag="ACH3L8_000638"
                     /pseudo
     CDS             29825..31267
                     /locus_tag="ACH3L8_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(31314..31727)
                     /gene="yidI"
                     /locus_tag="ACH3L8_000639"
     CDS             complement(31314..31727)
                     /gene="yidI"
                     /locus_tag="ACH3L8_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFT4083884.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            31836..32183
                     /locus_tag="ACH3L8_000640"
     CDS             31836..32183
                     /locus_tag="ACH3L8_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFT4083885.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32173..32535
                     /locus_tag="ACH3L8_000641"
     CDS             32173..32535
                     /locus_tag="ACH3L8_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFT4083886.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32532..33029
                     /locus_tag="ACH3L8_000642"
     CDS             32532..33029
                     /locus_tag="ACH3L8_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFT4083887.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33037..>34051)
                     /gene="emrD"
                     /locus_tag="ACH3L8_000643"
     CDS             complement(33037..>34051)
                     /gene="emrD"
                     /locus_tag="ACH3L8_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFT4083888.1"
                     /translation="VSHSCFGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIA
                     ASAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMW
                     NWRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLI
                     GGLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMW
                     QSVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAG
                     TAGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQG
                     QPV"
BASE COUNT         8000 a         8697 c         9126 g         8228 t
ORIGIN      
        1 agaaaatccg ccccagcagg tcgtctgaag taaattcccc ggtgatttcg cttaagttct
       61 gctgtgccag gcgcaactct tccgccagca gttcacctgc ccaggctccc aacagttgcg
      121 ctttaccttg ctgcaaatgc tccgctgcct gttccagcgc ctgtaagtgg cgacggcgcg
      181 ccaggaagcc gccttccata ttggtgtcaa agcccatgct ctgtttgaga tggttacgca
      241 gcacgtccac gccttcacca gtccttgccg agagacgaat taacgcgtga ccgttcactt
      301 cactcattcc cagcgtttcg ccggtgatat cggctttatt gcgtaccacg gtgatcggca
      361 gtttcgcggg cagacgggcg ataaattccg gccagatctc tgccggatca acggcgtctg
      421 tcgtggtgcc atcgaccata aacagcacgc ggtcggcctg ttcaatttcc tgccacgcgc
      481 gctcgatacc aatacgttcc acttcgtcac tggcctcacg tagcccggca gtgtcgatga
      541 tatgcagcgg cattccgtca atgtggatat gctcacgcag cacgtcacgc gtggttccgg
      601 cgatatcggt tacgattgcc gcttcacggc ccgccagcgc gtttaacagg ctcgatttac
      661 cggcattagg acgtccggca atcactactt tcatcccttc gcgcaacaaa ctaccctgac
      721 gtgcttcagc acgcactgca tcgagatcgg ccatcacgtc attaagctgg gcttcaattt
      781 tgccgtcgga gaggaaatcg atctcttcat cagggaaatc aattgccgct tcgacgtaaa
      841 tgcgcaagtg ggtgagggct tctaccagat gattaacccg tgcggagaat gcgccttgca
      901 gcgagttaag tgccgaacgg gccgcctgtt ccgaactggc gtcgataaga tcggcaatcg
      961 cctcggcctg ggctaagtca agtttatcgt taagaaacgc gcgttcggaa aactcaccag
     1021 ggcgagcaat ccgcaggccg ggaatggtca gaatgcgttt taacagcagg tcgaggatca
     1081 ccggaccgcc atgaccttgc agttccagca catcttcgcc ggtgaacgag ttcgggccag
     1141 ggaaccatag cgcaatcccc tgatcgagca cgctgccgtc ggcgtcttta aacggaagat
     1201 aatcggcgta gcgcggctta ggcagtttac ccagcacggt ttcggcaact tcacgggctt
     1261 tgaagccgga gatgcgcagg atgccaacgc cgccacgtcc cggaggcgtg gcctgggcta
     1321 cgatagtgtc attatcgctc atgatgttcc tgttgctttg tgtggcggat gcgcggtgct
     1381 tatccgccct acgaaaagaa aaaaggcggt caactgaccg cccttatttt agcgaaaact
     1441 caccgaatca ggattttttc ttctcgcggc tatgcaggcc acgtttttcc agaccacggt
     1501 aaatcagctg ctgctgaata atggttacca ggttgctgac gatatagtac agtaccagac
     1561 ctgacgggaa ccacaggaag aacacggtga agatgaccgg cataaaggtc atgatcttct
     1621 gctgcatcgg gtcggtcaca gtggtcggcg acatcttctg aatgaagaac atcgttacgc
     1681 ccatcaggat cggcaggatg tagtacgggt cctgtgccga caggtcgtgg atccacagtg
     1741 caaacggtgc ctgacgcagt tcaacggaac ccatcagcat gtagtacaac gccaggaaga
     1801 ttggcatctg gatcagcagc gggaagcagc cgcccagcgg gttaaccttc tcagctttgt
     1861 acagcgccat catttcctga ctgatacgct gtttgtcatc gcccagacgc tcacgcattg
     1921 cctgaatctt cggctgcagc atacgcatct tcgccatgga ggtgtactgc gctttggtca
     1981 gcgggtacat gatgccacga acgataaagg tgatgatgat aatggagaag ccccagttac
     2041 ccacaaagct atggatccat ttcagcagtt tgaacagcgg ctgagagatg aaccacaacc
     2101 aaccgtaatc aacggtcaga tccaggtgcg gagcaacagc tgccattttg tcctggattt
     2161 ccgggccaac ccacagggtg ctgttcatcg cgccagtctg accaggctga accagtaccg
     2221 gctgagattt atagccgata gcgacgatgc cgttacccag attagcggta tagaagttgt
     2281 tggtaccgtc gttatgcggg atccacgccg tcgcgaaata ctgttgcagc atcgccaccc
     2341 aaccaccttt cgaagagatg ttcaggtttt cgttatcggc aatggtatcg aacttgtatt
     2401 tctcatactt ctcgtcaggc gtggagtacg ccgcgccacg gaaggtgtgc agtgcgaagt
     2461 tgctgcttcc ggtatcgaga tgcggtggca gagtgatgga ttgcttcaac tgaccaaacg
     2521 aggagatttc cagcggtttc tcgccagcgt tctgcacgtt gtagttgacg ttgacagcgt
     2581 aatcaccacg tttcaggaca aacgttttgg taaacgtgtt gcctgccgcg tcggtatacg
     2641 tcatcggcac ctgcagttcg ttttgacctt cagccagcac ataagcgtct ttttcaacgt
     2701 tatacagcgg acgcgggccg ttagccgggt tatccgggcc atcacgaccg gtcagaccgc
     2761 tctgtgcctg ataaataaac tgcggtgaag tttccaacag ctggaacggc tgggtagagt
     2821 tcagctcttt cgggtaagca ggcagcagag cttgctcaac atcaccacca cgggtgttga
     2881 tggtcagatc aagcacgtcg gtcttaaccg agatcagttt cccctggcca ctggccggta
     2941 cgccctggtc ggcggcgcta cccgctgcgg tggtcgttgt ctgcgtggtc tgttgggcct
     3001 gaggttgcgg gtttttatcc tgctcccagg cttgccagat catgaaagac acgaacagca
     3061 aagcgatgac taaaagattg cgttgcgaat ccatcgttag tgttctctgg tatcaaatgg
     3121 tccgggcggg acgggatcgt caccaccagg gtgtaaaggg tggcatttta atacgcgttt
     3181 caccgtcaac caactgcctt ttatcactcc aaacctgcgc aatgcctcaa ttccgtagct
     3241 tgaacaggtt ggagtgaaac gacaatgcgg cccgagtagc ggactaatca ggcgttgata
     3301 gacccgaatg agggctatca ggacccgcga gccaggcgac agtggcggcg ccataatttt
     3361 tccaacgctt ccgagagagc acggttatcg aggtcggcaa cccctttttt cgccaccacc
     3421 acgaaatcca tagccgggag ttcatgttgg cgcagacgga agctttcacg cgtcaggcgt
     3481 ttaatccgat tgcgttcatg ggcgcgtcga acgtttttct tggcgactgt aagaccgata
     3541 cggggatgcc ccagcgaatt caggcggccg agaatggtaa tttgcggcgt gccagcccgt
     3601 tgtggctgct ggaagacgaa tgtgaattga ctgggagtta acaagcgtaa ctccctggga
     3661 aatgcgagct taaccactca ggggttagct ttattactta gaaacggtca gacgagcgcg
     3721 gcctttagca cgacgacgtg ccagaacctg acgaccattt ttagtagcca tacgagcacg
     3781 gaagccgtga gaacggttgc gcttcagtac agacggttga aaagtgcgtt tcatggcgat
     3841 ttctacctaa acttgaataa attcaatggc tttattggat atccgccgaa aaatgaaacg
     3901 atggacaccg aagccatggg tgattaaaga ggccggattg taataattgt acacaccgga
     3961 gtcaattctc tttccttatt taccgcgctt ttccgcacct tttcgcaggg aaaatgtacg
     4021 acctcacacc agtggaaacc agcatggcgc gccgggtgga ggattatacg ggctgatggg
     4081 taaagcgcaa ggatcgtcct ggatctttat tagatcgatt aagccaattt ttgtctatgg
     4141 tcattaaatt ttccaatatg cggcgtaaat cgtgcccgcc tcgcggcagg atcgtttaca
     4201 cttagcgagt tctggaaagt cctgtggata aatagggaaa atctgtgaga aacagaagat
     4261 ctcttgcgca gtttaggcta tgatccgcgg tcccgatcgt tttgcaggat cttgatcggg
     4321 catataaccg cagacagcgg ttcgtgcgtc accctcaagc agggtctttt cgacgtacgt
     4381 caacaatcat gaatgtttca gccttagtca ttatcgactt ttgttcgagt ggagtccgcc
     4441 gtgtcacttt cgctttggca gcagtgtctt gcccgattgc aggatgagtt accagccaca
     4501 gaattcagta tgtggatacg cccattgcag gcggaactga gcgataacac gctggccctg
     4561 tacgcgccaa accgttttgt cctcgattgg gtacgggaca agtaccttaa taatatcaat
     4621 ggactgctaa ccagtttctg cggagcggat gccccacagc tgcgttttga agtcggcacc
     4681 aaaccggtga cgcaaacgcc acaagcggca gtgacgagca acgtcgcggc ccctgcacag
     4741 gtggcgcaaa cgcagccgca acgtgctgcg ccttctacgc gctcaggttg ggataacgtc
     4801 ccggccccgg cagaaccgac ctatcgttct aacgtaaacg tcaaacacac gtttgataac
     4861 ttcgttgaag gtaaatctaa ccaactggcg cgcgcggcgg cttgccaggt ggcggataac
     4921 cctggcggtg cctataaccc gttgttcctt tatggcggca cgggtctggg taaaactcac
     4981 ctgctgcatg cggtgggtaa cggcattatg gcgcgcaagc cgaatgccaa agtggtttat
     5041 atgcactccg agcgctttgt tcaggacatg gttaaagccc tgcaaaacaa cgcgatcgaa
     5101 gagtttaaac gctactaccg ttccgtagat gcactgctga tcgacgatat tcagtttttt
     5161 gctaataaag aacgatctca ggaagagttt ttccacacct tcaacgccct gctggaaggt
     5221 aatcaacaga tcattctcac ctcggatcgc tatccgaaag agatcaacgg cgttgaggat
     5281 cgtttgaaat cccgcttcgg ttggggactg actgtggcga tcgaaccgcc agagctggaa
     5341 acccgtgtgg cgatcctgat gaaaaaggcc gacgaaaatg acattcgttt gccgggtgaa
     5401 gtggcgttct ttatcgccaa gcgtctacga tctaacgtac gtgagctgga aggggcgctg
     5461 aaccgcgtta ttgctaacgc caactttacc ggacgggcga tcaccatcga cttcgtgcgt
     5521 gaggcgctgc gcgacttgct ggcattgcag gaaaaactgg tcaccatcga caatattcag
     5581 aagacggtgg cggagtacta caagatcaaa gtcgcggatc tcctttccaa gcgtcgatcc
     5641 cgctcggtgg cgcgtccgcg ccagatggcg atggcgctag cgaaagagct gactaaccac
     5701 agtctgccag agattggcga tgcgtttggt gggcgtgacc acacgacggt gcttcatgcc
     5761 tgccgtaaga tcgagcagtt gcgtgaagag agccacgata tcaaagaaga tttttcaaat
     5821 ttaatcagaa cattgtcatc gtaaacctat gaaatttacc gtagaacgtg agcatttatt
     5881 aaaaccgcta caacaggtga gcggtccgtt aggtggtcgt cctacgctac cgattctcgg
     5941 taatctgctg ttacaggttg ctgacggtac gttgtcgctg accggtactg atctcgagat
     6001 ggaaatggtg gcacgcgttg cgctggttca gccacacgag ccaggagcga cgaccgttcc
     6061 ggcgcgcaaa ttctttgata tctgccgtgg tctgcctgaa ggcgcggaaa ttgccgtgca
     6121 gctggaaggt gaacggatgc tggtgcgctc cgggcgtagc cgtttttcgc tgtctaccct
     6181 gccagcggcg gatttcccga acctcgatga ctggcagagc gaagtcgaat ttaccctgcc
     6241 gcaggcgacg atgaagcgtc tgattgaagc aacccagttt tcgatggcgc atcaggatgt
     6301 tcgctattac ttaaacggta tgctgtttga aaccgaaggt gaagaactgc gcaccgtggc
     6361 aaccgacggc caccgtctgg cggtctgttc aatgccaatt ggtcaatctt tgccaagcca
     6421 ttcggtgatc gtaccgcgta aaggcgtgat tgaactgatg cgtatgctcg acggcggcga
     6481 caatctgctg cgtgtgcaga ttggcagcaa taacattcgt gcccacgttg gcgactttat
     6541 tttcacctcg aaactggtgg atggtcgctt cccggattac cgtcgcgttc tgccgaagaa
     6601 cccggacaaa catctggaag caggctgcga tctgctcaag caggcgtttg ctcgcgcggc
     6661 gattctctct aacgagaaat tccgcggtgt gcgcctgtat gtcagcgaaa accagctgaa
     6721 aattactgcc aacaacccgg aacaggaaga agcggaagag atcctcgacg ttacctatag
     6781 cggtgcggag atggaaatcg gcttcaacgt cagctatgtg ctggatgttc tgaacgcgct
     6841 gaaatgcgaa aacgtccgca tgatgctgac cgattcggtt tccagcgtgc agattgaaga
     6901 tgccgcatca cagtcggctg cctatgttgt catgccaatg agactgtaat gtccctcacc
     6961 cgcttgttga tccgcgattt ccgcaatatt gaaaccgcgg atctcgcttt atctcccggc
     7021 tttaactttc tggtaggtgc caacggcagt ggcaaaacca gcgtgctgga agccatctat
     7081 acgctcggcc atggtcgggc gtttcgcagt ttgcagattg gtcgcgtcat tcgccatgag
     7141 caggaggcat ttgttctcca cgggcgatta cagggcgaag agcgcgagac ggcgattggc
     7201 ttaaccaaag acaaacaggg cgacagcaaa gtccgtattg acggtacgga cgggcataaa
     7261 gtcgcggaac tggcgcacct gatgccgatg cagttgataa cgccagaagg gtttacttta
     7321 ctcaacggcg gccccaaata cagaagagca ttcctcgact ggggatgctt tcacaacgaa
     7381 cctggatttt tcaccgcctg gagcaatctc aagcgattgc tcaagcagcg caatgcggcg
     7441 ctgcgccagg tgactcgtta cgaacagcta cgcccgtggg ataaagaact gatcccgctg
     7501 gcggagcaaa tcagcacctg gcgcgcggag tatagcgccg gtatcgcggc cgatatggat
     7561 gatacctgta agcaatttct ccctgagttt tctctgactt tctctttcca gcgcggctgg
     7621 gagaaagaga cagagtacgc tgaggtgctg gaacgtaatt ttgaacgcga tcgccagcta
     7681 acctacaccg cgcatggccc gcataaagcg gacttacgca ttcgcgccga cggtgcgccg
     7741 gtggaagata ccttatcgcg tgggcagctt aagctgttga tgtgcgcctt acgtctggcg
     7801 caaggagagt tcctcactcg tgaaagcggg cggcggtgtc tctacctgat agatgatttt
     7861 gcctctgagc ttgatgatga gcgtcgcggg ttgcttgcca gccgcttaaa agcgacgcaa
     7921 tcacaggtct ttgtcagcgc gatcagtgct gaacacgtta tagacatgtc ggacgaaaat
     7981 tcgaagatgt ttaccgtgga aaagggtaaa ataacggatt aacccaagta taaatgagcg
     8041 agaaacgttg atgtcgaatt cttatgactc ctccagtatc aaagtcctga aagggctgga
     8101 tgcggtgcgt aagcgcccgg gtatgtatat cggcgacacg gatgacggca ccggtctgca
     8161 ccacatggta ttcgaggtgg tagataacgc tatcgacgaa gcgctcgcgg gtcactgtaa
     8221 agaaattatc gtcaccattc acgccgataa ctctgtctct gtacaggatg acgggcgcgg
     8281 cattccgacc ggtattcacc cggaagaggg cgtatcggcg gcggaagtga tcatgaccgt
     8341 tctgcacgca ggcggtaaat ttgacgataa ctcctataaa gtgtccggcg gtctgcacgg
     8401 cgttggtgtt tcggtagtaa acgccctgtc gcaaaaactg gagctggtta ttcagcgcga
     8461 gggtaaaatt caccgtcaga tctacgaaca cggtgtaccg caggctccgc tggcggttac
     8521 cggcgagact gaaaaaaccg gcaccatggt gcgtttctgg cccagcctcg aaaccttcac
     8581 caatgtgacc gagttcgaat atgaaattct ggcgaaacgt ctgcgtgagt tgtcgttcct
     8641 caactccggc gtttccattc gtctgcgcga caagcgtgac ggcaaagaag accacttcca
     8701 ctatgaaggc ggcatcaaag cgttcgttga atatctgaac aagaacaaaa cgccgatcca
     8761 cccaaatatc ttctacttct ccaccgaaaa agacggtatt ggcgttgaag tggcgctgca
     8821 gtggaacgat ggcttccagg aaaacatcta ctgctttacc aacaacattc cgcagcgtga
     8881 cggcggtact cacctggcag gcttccgtgc ggcgatgacc cgtaccctga atgcctacat
     8941 ggacaaagaa ggctacagca aaaaagccaa agtcagcgcc accggtgacg atgcgcgtga
     9001 aggcctgatt gcggtcgttt ccgtaaaagt gccggacccg aaattctcct cccagaccaa
     9061 agacaaactg gtttcttctg aggtgaaatc ggcggttgaa cagcagatga acgaactgct
     9121 ggcggaatac ctgctggaaa acccaactga tgcgaaaatc gtggttggca aaattatcga
     9181 tgctgcccgt gcccgtgaag cggcgcgtcg cgcgcgtgaa atgacccgcc gtaaaggtgc
     9241 gctcgactta gctggcctgc cgggcaaact ggcagactgc caggaacgcg atccggcgct
     9301 ttccgaactg tacctggtgg aaggggactc cgcgggcggc tctgcgaagc aggggcgtaa
     9361 ccgcaagaac caggcgattc tgccgctgaa gggtaaaatc cttaacgtcg agaaagcgcg
     9421 cttcgataag atgctctctt ctcaggaagt ggcgacgctt atcaccgcgc ttggctgtgg
     9481 tatcggtcgt gacgagtaca acccggacaa actgcgttat cacagcatca tcatcatgac
     9541 cgatgcggac gtcgacggct cgcacattcg tacgctgctg ttgaccttct tctatcgtca
     9601 gatgccggaa atcgttgaac gcggtcacgt ctacatcgct cagccgccgc tgtacaaagt
     9661 gaagaaaggc aagcaggaac agtacattaa agacgacgaa gcgatggatc agtaccagat
     9721 ctctatcgcg ctggacggcg caacgctgca caccaacgcc agtgcaccgg cattggctgg
     9781 cgaagcgtta gagaaactgg tatctgagta caacgcgacg cagaaaatga tcaaccgcat
     9841 ggagcgtcgt tatccgaaag caatgctgaa agagcttatc tatcagccga cgttgacgga
     9901 agctgacctt tctgatgagc agaccgttac ccgctgggtg aacgcgctgg tcagcgaact
     9961 gaacgacaaa gaacagcacg gcagccagtg gaagtttgat gtccacacca atgccgaaca
    10021 aaacctgttc gagccgattg ttcgcgtgcg tacccacggt gtggatactg actatccgct
    10081 ggatcacgag tttatcaccg gtggcgaata tcgtcgtatc tgcacgctgg gtgagaaact
    10141 gcgtggcttg ctggaagaag atgcgtttat cgaacgtggc gagcgtcgtc agccggtagc
    10201 cagcttcgag caggcgctgg actggctggt gaaagagtcc cgtcgtggcc tctccatcca
    10261 gcgttataaa ggtctgggcg agatgaaccc ggaacagctg tgggaaacca ccatggaccc
    10321 ggaaagccgt cgtatgctgc gcgttaccgt taaggatgcg attgccgctg accagttgtt
    10381 caccacgctg atgggcgacg ccgtagaacc gcgtcgtgcg tttattgaag agaacgccct
    10441 gaaagcggcg aatattgata tttaatggcg ttaaccatgc gagcgtgcct gatgcgctac
    10501 gcttattagt cctacgagac tcctgcaata tattgatttt gcatgatttt gtaggccggg
    10561 taaggcgttc acgccgcatc cggcaagaag ctataagaaa agggcggaga tcatctccgc
    10621 cctttttatt tctgcaatcc gcaattatca tcccgcagat ctatactttt tgggtcaaca
    10681 cgtaacgctt tacaggtgat tatcatgggg ctttttgatg aagttgtcgg tgcctttctg
    10741 aaaggcgatg cggggaaata tcaggcgatt ttaagttggg ttgaggagca gggtggcatt
    10801 caggtgctgc tggaaaaact gcaaagtggc ggcttagggg ccattctctc aacctggttg
    10861 agtaatcaac agagaaatca atcagttagc ggcgagcaac tggagtcggc gctcggcaca
    10921 aatgcggtgt ccgatctcgg gcaaaaactt ggcgtggata ccagtacggc ttccagttta
    10981 ctggcagagc aattgccgaa gattattgat gcgctctcac cgcaaggtga agtgtcagca
    11041 caagccaata acgatctgct ttccgcaggc atggaactgc tgaaagggaa actcttccgc
    11101 taagcaaatg gggagcatgc aacccgcgtt gcatcccctt ttccctcggg tgttgtactg
    11161 atttttgagc ggaatcgcgt tagcatgggt caggaactaa tctaccttgg gaactcatgg
    11221 ctatcaaact cattgctatc gatatggatg gcacccttct gctgcccgat cacaccattt
    11281 cacccgccgt taaaaatgcg attgccgcag ctcgcgcccg tggcgtgaat gtcgtgctaa
    11341 cgacgggtcg tccgtatgca ggtgtgcaca actacctgaa agagctgcat atggaacagc
    11401 cgggcgacta ctgcattact tataacggcg cgctggtaca gaaggccgct gatggtagca
    11461 ccgtggcgca aaccgctctc agctatgacg actatcgttt cctggaaaaa ctctctcgcg
    11521 aagtcggttc tcatttccac gccctggacc gcaccacgct gtacaccgct aaccgcgata
    11581 tcagctacta cacggtgcat gaatccttcg ttgccacaat tccgctggtg ttctgcgaag
    11641 cggagaaaat ggaccccaat acccagttcc tgaaagtgat gatgattgat gaacccgcca
    11701 tcctcgacca ggctatcgcg cgtattccgc aggaagtgaa agagaaatat accgtgctga
    11761 aaagtgcgcc gtacttcctc gaaatcctcg ataaacgcgt taacaaaggt acgggtgtga
    11821 aatcactggc cgacgtgtta ggtattaaac cggaagaaat catggcgatt ggcgatcagg
    11881 aaaacgacat cgcaatgatt gaatatgcag gcgtcggtgt ggcgatggat aacgctattc
    11941 cttcggtgaa agaagtggcg aactttgtca caaaatccaa ccttgaagat ggcgtggcgt
    12001 ttgctattga gaagtatgtg ctgaattaat ctgtgggcgg gcaaacagca taaacgcgtt
    12061 tgcccgctta ctgattattg gtttactgca tatacaacca gaaattgcag cggtgcagta
    12121 atgatgtcca gtgtaacagt cactggcagt aatgctgcgg aaacaccatc cggaatgcct
    12181 ctgggtgaat agtattcata aaatcgcact ttaaatggat ggtagaacgc catcaccttt
    12241 gagtaatcca tgtttttatt tttctcatgg attgtgcctt ttaggttttc cagtgacctg
    12301 gtacagtgtt gaacaccaca ggcaaaacca taatgcgttg ccagtgcctt ttcttcttcg
    12361 ttattccagt agtagtaaag cgatattgtg ccggtaaatt tctttttccc cgcatttaaa
    12421 acaaatcttg cgtcatcggc aacctgaata ttgtgccggt taagtgctgg atcattgagc
    12481 atcttaacga tgtcatcggc accatcagtg ataaggtaat ccagcgattc ccctacaaaa
    12541 acatagcctt ttgttccatt actatctttt gcctgcgata gcccagtgat ggtgtctgac
    12601 tgcgattgat actttcctga actacccttt gcggtatggg aaaccagagc atacgagata
    12661 cagcctgaaa gcatcagcgc cagtgggaat aaaccggcag ccttaaaaaa tcccttaaaa
    12721 ttcaacttca tgattaatcc tttaaacgag tagctaagcc gttgaaagca tactcttctt
    12781 tccctcctgt ttagcgcctg atatcccttt tcagatttct gcccgacgca tgtcattttt
    12841 ttatgtgttg ttcttttttt gatctaaatt gtagcgcaat aatataagtt tgtactacat
    12901 tacgcgcgcg gcaaacgcga acgtcatcac gctggtacta caaagttgcc gcgttatgca
    12961 tcgatcgggg taaagtagag aagaacatac agagcacaag gactctccat gactctcaat
    13021 aaaaccgatc gcattgtcat tacgctgggt aaacagatcg ttcacggcaa atacgtacct
    13081 ggctcgccac ttccggctga ggcggagctc tgtgaagagt ttgcaacctc gcgcaacatc
    13141 atccgtgagg tgttccgttc gctgatggcg aagcggctga ttgaaatgaa acgttatcgc
    13201 ggggcgtttg tggcaccgcg taaccagtgg aattacctcg acactgacgt actgcaatgg
    13261 gtgctggaaa acgactacga cccacggctt atcagtgcca tgagcgaagt gcgaaatctg
    13321 gtggaaccgg cgattgcccg ttgggcagca gagcgcgcga cctccagcga tctggcgcag
    13381 attgaatcgg cgctgaacga gatgattgcc aacaatcagg accgcgaagc gtttaacgaa
    13441 gcggatattc gctaccacga ggcggtgctg cagtcggtgc ataacccggt gttacagcaa
    13501 cttagcattg cgatcagttc gctgcagcgg gcggtttttg aacgaacctg gatgggcgat
    13561 gaggccaaca tgccgcaaac gctccaggaa cataaggcgc tgttcgatgc gatacggcat
    13621 caggacggcg atgcggcaga gcaggcggcg cttaccatga ttgccagctc gacacgaagg
    13681 ttaaaggaaa tcacatgaca gctcgctaca tcgcaattga ctggggatcg accaatctgc
    13741 gcgcctggct ttatcagggc gaccactgcc tggagagcag gcaatcagaa gcaggcgtca
    13801 cgcgcctgaa cggaaaatct ccggctgcag tgttagcaga agtcacgacc gactggcgtg
    13861 aagaaaatac gccagtggtg atggcaggaa tggtcggcag caatgtcggc tggaaagttg
    13921 ctccgtattt atctgttcct gcccgttttt cgtctattgg cgaacaatta acgtctgttg
    13981 gcgacaatat ctggattatt cccggattat gcgtctctca tgacgataac cacaatgtga
    14041 tgcgcggcga agaaacacaa ttgatcggcg cgcgagctct ggctccttcc tctctttatg
    14101 ttatgcccgg tacgcattgc aaatgggtgc aggccgatag ccagcaaatc aacgattttc
    14161 gcaccgtgat gaccggtgaa ttacatcatt tactgttaaa tcactcattg attggcgcag
    14221 gtttgccgcc gcaggaaaac tctgccgatg ccttcgcggc tggccttgag cgcggcctta
    14281 atgcgcccgc catattgccg cagctttttg aagttcgcgc ctcgcatgtg ctgggaacac
    14341 ttccccgcga acaggtcagc gaatttctct ctggtttgtt gattggcgca gaggttgcca
    14401 gtatgcgcga ctatgtgacc catcaacacg ccatcaccct tgtcgccgga acatcgctga
    14461 ccgcgcgcta ccagcaagcc tttcaggcga tgggttgcga cgtggcggcg gtggcgggcg
    14521 acacggcatt tcaggctggt ataaggagca tcgctcatgc agtggcaaac taaactcccg
    14581 ctgatcgcca ttttgcgcgg cattacgccc gacgaggcgc tggcgcatgt tggcgcggtg
    14641 attgacgccg ggttcgacgc ggttgaaatc ccgctgaatt ccccacaatg ggagcagagc
    14701 attcccgcca tcgttgatgc gtatggcgac aaggcgttga ttggcgcagg tacggtactg
    14761 aaacctgaac aggtcgatgc gctcgccagg atgggctgtc agctcatcgt tacgcccaat
    14821 atccatagtg aagtgatccg ccgcgcggtg ggctacggca tgaccgtttg ccccggctgc
    14881 gcgacggcga ccgaagcctt taccgcgctc gaagcgggcg cgcaggcgct gaaaatattt
    14941 ccgtcatcgg cttttggtcc gcaatacatc aaagcgttaa aagcggtatt accaccggac
    15001 atcgcagtct ttgccgttgg cggcgtgacg ccagaaaacc tggcgcagtg gatagacgca
    15061 ggttgtgttg gggcgggctt aggcagcgat ctttatcgcg ccgggcagtc cgtagaacgc
    15121 accgcgcagc aggcagcagc atttgttaag gcgtatcgag aggcagtgca atgaaaatca
    15181 ccaaaattac cacgtatcgt ttacctcccc gctggatgtt cctgaaaatt gaaaccgatg
    15241 aaggcgtggt cggttggggc gagcccgtga ttgaaggccg cgcccgtacg gtggaagcgg
    15301 cagttcacga gctgggtgac tatttgattg gtcaggatcc ttcgcgcatc aacgacttat
    15361 ggcaagtgat gtatcgcgcc ggattttatc gtggcggtcc aatcctgatg agcgccatcg
    15421 ccgggatcga ccaggcgtta tgggatatca aaggcaaagt gctgaatgcg ccagtctggc
    15481 aactgatggg cggcctggtt cgcgacaaaa ttaaagccta cagttgggtc ggcggcgatc
    15541 gtccggcgga tgttatcgac ggcattaaaa ccctgcgcga aatcggcttc gataccttca
    15601 aactgaacgg ttgtgaagaa ctggggctaa ttgataactc ccgcgcggta gatgcggcag
    15661 tcaacaccgt ggcacaaatt cgtgaagctt ttggcaatca gattgagttt ggtcttgatt
    15721 tccacggtcg cgtcagcgcg ccaatggcga aagtgctgat taaagaactg gagccgtatc
    15781 gcccgctgtt tattgaagag ccggtgctgg cggaacaagc cgaatactac ccgaaattgg
    15841 cggcacaaac gcatattcca ctggcggcgg gtgagcgcat gttctcacgt ttcgatttta
    15901 aacgcgtgct ggaggcaggc ggtatttcga ttctgcaacc ggatctctcc catgcaggcg
    15961 gtattaccga atgctacaaa attgctggaa tggcagaagc ctatgatgtg acccttgcgc
    16021 cgcactgtcc gttcggaccg attgcactgg cggcttgcct gcatatcgac tttgtttcct
    16081 ataacgcggt acttcagtaa caaagtatgg gcattcatta caacaaaggc gcggagttac
    16141 tcgactttgt gaaaaacaaa gaagacttca gcatggttgg cggcttcttt aaaccgttaa
    16201 cgaaaccggg cttaggtgtg gaaatcgacg aagctaaagt tattgagttc agtaaaaatg
    16261 ccccggactg gcgtaatccg ctctggcgtc atgaagataa cagcgtagca gagtggtaat
    16321 tcctgccacg taagcccctc atcgggcact aaaacagcaa tacaaaaata taaccctctg
    16381 taaattacag ggcatggtga gcggcttcgc tatgcccaaa atctggagaa agctcgctat
    16441 ggatattccc gttaatgcag caaagccggg gcgtcggcgt tatctgacgc tggtgatgat
    16501 ctttattacg gtggtcattt gttatgttga ccgcgctaac ctggccgtgg cttccgccca
    16561 tattcaggaa gagttcggca ttacgaaagc ggaaatgggc tatgtatttt cggccttcgc
    16621 ctggctttat acgctatgcc agatccccgg cggttggttt ttagatcgcg taggttctcg
    16681 cgtgacttat tttattgcga tatttggttg gtcagtggcg actttattcc aaggctttgc
    16741 cacgggatta atgtcattaa ttggtctgcg tgcgataacc ggtattttcg aagcgcccgc
    16801 tttcccgacc aataaccgga tggtgaccag ctggttcccg gaacatgaac gcgcttccgc
    16861 cgttggtttt tatacgtctg gtcagtttgt cggtctggcg tttttgactc cgctgctgat
    16921 ctggattcag gagatgttga gctggcactg ggtgttcatt gtcactggtg gtatcggcat
    16981 tatctggtcg ctgatttggt ttaaggttta tcagccgccg cgcctgacca aaggtatcag
    17041 caaagctgaa ctggattaca ttcgtgatgg cggcggtctg gtggatggtg atgcgccggt
    17101 gaagaaagag gcgcgtcagc cgttaacagc caaagactgg aaactggtgt tccatcgtaa
    17161 actgatcggc gtttatcttg ggcaatttgc ggtggcttct gcactgtgtt ttttcttaac
    17221 ctggttcccg aactatttaa cccaggaaaa aggaatcacg gcgctgaaag caggctttat
    17281 gaccacggtg ccattcctcg cggcgtttgt cggcgtcctg ctctctggct gggtcgcgga
    17341 tctgctggta cgtaagggct tttcactggg ctttgcgcgt aaaacgccga ttatctgcgg
    17401 cttgctgatc tccacctgca ttatgggcgc taactacacc aacgatccga tgatgattat
    17461 gtgcctgatg gcgctggcat tcttcggcaa cggttttgct tcgattacct ggtcgctggt
    17521 ctcttctctg gcaccgatgc gcctgattgg tttaaccggc ggcgtgttta acttcgccgg
    17581 tggtctgggc ggcatcaccg ttccgctggt ggtggggtac ctggcgcagg gttacggttt
    17641 cgcgcctgca ctggtttata tctccgccgt cgcgttgatt ggcgcgctct cttacatcct
    17701 gctggtgggt gatgtgaagc gcgttggata atctttcaac tgtggaatat gcgccacacc
    17761 actgcgcata atccactttc ttaaagccgg tgcggtcagg catcggcttt ttactatctt
    17821 gccgaagagt tttaaacgca gtttgtgtgt ggcgcgccag taagcggcgt tgctcttctc
    17881 cggctgtttc aggaacaccg cacgcagaat ctctgcgcta tccagcgcat agctaatccc
    17941 ctccagcgag ctggcgctga taaatcccgc cgcttcacca atcagaaagg cgttgtcctt
    18001 accacagaca aaatcctgcc agcgtgaggg aaacaacacc gtgcatttct cacttttcac
    18061 cgctttgcca aactggaact gaaaggcgct cattttctct ttcagcgtcg tgaaacgcgt
    18121 ctgaccgtct ttcattggat aggcaccgcc aaagataaaa taaccatctt tgctgatact
    18181 ccatgagtaa cagtcagtta tcgcattatc aaagatgcag gagtagaacg gcaccggatg
    18241 tttctctgcg aaccactgct ggatagcgac atatttacgg atttgatgat ccgggtagag
    18301 atgtcgccgc accatcgagt ttgcgccatc ggcaccgacc agatagcggg cggtaatatg
    18361 ttgctcccag ccgtctgcac gaaaaatgac atgccattta tcatcctcac gccagatttt
    18421 tcggcacagg ctatcgtgat aaacctcaac gctggcgggg atcagcgatt tcatccacaa
    18481 gtcgaaagca tggcgattaa tattgatata gcttcgctgg tagttacgcg tgagcgatgc
    18541 ggcgacgtcg acagttttga cgctgaaaat ctgtggattg gcgatcacat cgacaggaag
    18601 cgtcagtcca tcgcgaataa aagatcgctg cgcgtccggt gccagcagac cgccgcaagg
    18661 tttgctgaaa ccttcagtac cgcactggtg ctttttatcc agcgcgatca cctgcatttt
    18721 gccggctaac tttcgtgcca acgccgaccc tgccgggccg aggccaataa tcgccacgtc
    18781 gaaatgttcc atcttttcac tcacattcat cacgatggca aaagactatt gtctgaaagt
    18841 gaagtccttg tgaagtatga tggcgggaac cgttttgtat caggaccgcg ccatgaaaca
    18901 gataaccttt gctccccgta atcacctgct caccaatacc aataccaata ccaataccaa
    18961 taccaatacc tggacgcccg acagccagtg gctggtattt gacgtgcgtc cttctggggc
    19021 gtcgtttacc ggcgagacca ttgagcgtgt gaatatccat acaggcgagg tcgaggttat
    19081 ctatcgcgcg tcacagggcg cacacgtcgg cgtggtgacc gttcatccga agtcagagaa
    19141 atatgtcttt attcacggcc ctgaaaatcc tgatgaaaca tggcattacg acttccatca
    19201 tcgacgcgga gtcattgctg aaggcggcaa ggtgagcaat ctcgatgcaa tggatattac
    19261 cgcgccgtat acgccaggag cgctgcgcgg tggcagccat gtgcatgtct ttagcccgaa
    19321 cggtgaaagg gggagcttta cctataacga ccatgtaatg catcaactcg atccggcgct
    19381 ggatttgcga aacgtcgggg ttgctgcacc gtttggcccg gtcaacgtac aaaagcaaca
    19441 tccgcgtgaa tacagcggta gccactggtg tgtgctggtg agtaaaacca cgcccacgcc
    19501 gcagcctggc agtgatgaaa tcaatcgtgc ttatgaagaa ggatgggtag gaaatcacgc
    19561 gctggcgttt attggcgaca cactttcgcc aaagggcgag aaagtgccgg agctatttat
    19621 cgttgagtta ccgcaagatg aagctagctg gaaatcggca ggtgatgcgc cgttaagtgg
    19681 aacggaaaca accctacccg cgccaccgcg tggcgtcgtg cagcgacgtt taacctttac
    19741 ccatcatcgg gcttatccgg ggttagtcaa cgtcccgcgc cactgggtgc gctgtaatcc
    19801 gcagggtacg caaatcgcgt ttttaatgcg tgatgataac ggcattgtgc aactgtggct
    19861 tatctcgcca cagggcggcg aaccgcgcaa gttaacccat aacaaaacgg atattcagtc
    19921 tgcatttaac tggcatccgt caggagaatg gttgggcttt gtgctggata atcgaattgt
    19981 ttgtgcccat gcgcaaagcg gcgaggttga gtatttaacc gaaaaccacg ccaatccacc
    20041 ttctgcggac gccgtggtct tctcgccgga tggtcaatgg ctggcgtgga tggaaggtgg
    20101 ccagctgtgg atcaccgaaa ctgatcgcta aatcagttat caggcattct ggccggtgga
    20161 ataaccgcat tggtggcttg cgcgttggct tcgctttttt caacgcgcga gcgcaccgag
    20221 ctgtctttgc ggaacacatc ccacggcagt aaaagcgtgt ccattacagc ggtaaacggc
    20281 atatcgagaa tcgccagcga cttagtgccc cagttggttt catcgtcgcc aatcatggtg
    20341 gcgctggcgc gggtgccggg atacgttcct tctttccccc cggtatggga catcacgctg
    20401 gagcatcctc cgagtaatgt cattccgctg catatcatga acgctaacaa cacatttctt
    20461 atcataataa attcatctgt tgatcgtggg tgttggcctg atgagttata gcgatccctt
    20521 gctgaaaata acatcatcat tacgtcgcac tgtggcggct atcgcacttt aacgtttcgt
    20581 gctgccccct cagtctatgc aatagaccat aaactgcaaa aaaaagtccg ctgataaggc
    20641 ttgaaaagtt catttccaga cccattttac atcgtagtcg atgaggacgc gcctgatggg
    20701 tgttctggct acctgacctg tccattgtgg aaggtcttac attctcgctg atttcaggag
    20761 ctattgatta tgcgtaactt tgatttatcc ccgctttacc gttctgctat tggatttgac
    20821 cgtttgttta accacttaga aaacaaccag agccagagta atggcggcta ccctccgtat
    20881 aacgttgaac tggtagacga aaaccattac cgcattgcta tcgctgtggc tggttttgct
    20941 gagagcgaac tggaaattac cgcccaggat aatctgctgg tggtgaaagg tgctcacgcc
    21001 gacgaacaaa aagagcgcac ctatctgtac cagggcatcg ctgaacgcaa ctttgaacgc
    21061 aaattccagt tagctgagaa cattcatgtt cgtggggcta acctggtaaa tggtttgctg
    21121 tatatcgatc tcgaacgcgt gattccggaa gcgaaaaaac cgcgccgtat cgaaatcaac
    21181 taattcccta aggccgcctg gcgcggcctg acatctccat gctcgccgtc agggagcata
    21241 tgcgaatctt cggatttgca ggtacttact cgcttcttag aaggagaaat gactatgcgt
    21301 aacttcgatt tatccccact gatgcgtcaa tggatcggtt ttgacaaact ggccaacgca
    21361 ctgcaaaacg ccggtgaaag ccagagcttc ccgccgtaca acattgagaa aagcgacgat
    21421 aaccactacc gcattaccct tgcgctggca ggtttccgtc aggaagattt agagattcaa
    21481 ctggaaggca cgcgcctgag cgtaaaaggc acgccggagc agccaaaaga agagaaaaaa
    21541 tggctgcatc aagggcttat gaatcagcca tttagcctga gttttacgct ggctgaaaat
    21601 atggaagtct ctggcgcaac cttcgtaaac ggtttactgc atattgattt aattcgtaat
    21661 gagcctgaac ccatcgcagc gcagcgtatc gctatcagcg aacgtcccgc gttaaatagc
    21721 taactagcta ttctctttgc cccgccattc tggtggggct ttttttgtct ttcttcttca
    21781 acaatgcgtt aaatttcgac tgtttaagat atttcggcac gttctttccc gtgataatgt
    21841 gcgctataag gcaaatctgc ttcaccgatt cagccataat ccgtagcaat taaaatgtta
    21901 tctcaagaga aatggacgat gagtgatata gcattaacgg tcagtattct ggctttggtg
    21961 gcagtcgtcg gtttgtttat cggcaacgtc aaatttcgcg gcataggatt aggtattggc
    22021 ggcgtgctgt ttggtgggat catcgtcggc cattttgttt ctcaggcggg gatgacatta
    22081 agtagcgata tgctgcatgt tattcaggaa tttggcctga tcctgttcgt ttataccatc
    22141 gggattcagg tagggccggg cttctttgcc tcattgcgcg tctccggatt acgcctcaac
    22201 ctgtttgctg ttctgatcgt catcatcggt ggtctggtta ccgccatcct gcataaactg
    22261 tttgatattc cactgccggt agtgctgggg attttctccg gtgcggtaac caatacgcca
    22321 gcgctggggg cagggcagca gatcttgcgc gacctgggta caccaatgga aatggtcgat
    22381 cagatgggga tgagttacgc gatggcgtat ccattcggca tttgcgggat tttgttcacc
    22441 atgtggatgt tgcgggttat tttccgcgtc aatgtcgaga cagaagctca gcagcacgag
    22501 tcttcacgca ccaatggcgg cgcgctgatc aagactatca atattcgcgt tgagaaccct
    22561 aacctgcatg atttagccat taaagatgta ccgattctca acggcgacaa aattatctgc
    22621 tcgcgtctga aacgagaaga aaccctaaaa gtaccttcgc cagataccat tatccaactg
    22681 ggcgatttgc tgcatctggt ggggcagcca gcggatttac ataatgcgca actggtgatt
    22741 ggtcaggagg tcgatacctc gctgtctacg aaaggcactg atttgcgcgt cgagcgtgtg
    22801 gtggtcacca atgaaaacgt gctcggaaaa cgtattcgcg acctgcactt taaagaacgc
    22861 tatgacgttg ttatctcgcg cctgaaccgt gccggggtcg aactggtcgc cagtggcgat
    22921 atcagcctgc agttcggcga tatcctcaac ctggtggggc gtccgtccgc aattgatgcc
    22981 gttgccaatg tgctggggaa tgcgcagcaa aaactgcaac aggttcagat gctgccagtg
    23041 tttattggca tcgggcttgg cgtattgtta ggttctattc ccgtctttgt gccaggattc
    23101 ccggccgcgt tgaaactggg gctggcgggc ggtccgctga ttatggcgtt gatcctcggg
    23161 cgtatcggca gtatcggcaa gctgtactgg tttatgccgc caagtgccaa cctcgcgctg
    23221 cgggagctgg ggatcgtgct gttcctctcg gtcgttggtc tgaaatctgg tggggatttt
    23281 gtgaataccc tggtcaatgg cgaagggcta agctggattg gttatggtgc cctgatcacc
    23341 gccgttccgc tgattactgt tggcattctg gcgcggatgt tagccaaaat gaattacctg
    23401 accatgtgcg ggatgctggc aggttccatg accgatcctc cggcgctggc gtttgctaat
    23461 aatcttcatc caaccagtgg tgcggcggcg ctctcttacg ccactgtcta tccgttagtg
    23521 atgttcctgc gcattatcac cccccaatta ctggcggtgc tcttctggag tatcggttaa
    23581 cttttttccg ggtggagtcg ccgtaaatgg tactccacct agtattcgct ggcatttctg
    23641 aacatcactg aataattcaa aaactccccg ctctcgctat atgacaacga ggtgatccgc
    23701 agcagtggcg tgtgttccgc taccttcatt tgccgcgcca gcagtctatc ggcgagcacc
    23761 ggcatcaggc tttcataatt accgccaatc aaaatcccgc actcttgttc aatatattca
    23821 aacttcgacc cttccagatg ttgcagcgaa aggttacgga acagttttac cggcatatag
    23881 ctgtcttcca gcattagcgg tttcccttcc acaaaacgaa cgcgacggga gaagtagatc
    23941 tgctcgttga tttgaatccg taactggctg gcaatggccg gaggcgcagg catgatttca
    24001 aaaatcagca cctggctggt gaccgttttt ccctgccgtt ttaacacctc caccagtccg
    24061 gtcaggctgg cggtttgatg cagcacatct ttgcgcacca ggtaagtgcc gctgccgtgg
    24121 cggcggacca ccagccccca cgctaccagc aggtcaatgg ctttacggat agtcatccgc
    24181 gataccgcaa actcttccgc cagctttttt tcaccgggaa gaaggctgtt tagcgtgaaa
    24241 tccgcggaat taagtcgaat tcttaaccgc tccgcaatgc ttttgtagat catctctagc
    24301 tctctttttt gcctgcaagc acgggtaatg cgcccacaag ttctgcttaa tcgattgaaa
    24361 aaaatctttt ttttaaagat gtgttcgatg ctgtgtacct gtgctcacag atgtctactt
    24421 tttcgcgaaa acgtagatct ctaccgccca acgaaaagca tgaaagcgat cacgaatccc
    24481 attgggtcgt catgttctgc cgatcgcatc ttcctatcct cgctccaggt ctgccgcata
    24541 accaatcagg cttcctactt acagaattga gaaaagagga tgtggaaatg ctcagtcaaa
    24601 ttcaacgctt tggcggcgcg atgttcacgc cagtgctgct gtttcccttc gccgggattg
    24661 tggtgggtct tgccatcttg ctgcaaaacc cgatgtttgt cggggaatca ctgaccgatc
    24721 cgaacagttt attcgcgcaa atcgtacaca ttattgaaga gggcggttgg acggtattcc
    24781 gtaatatgcc gctgattttt gctgtcggtt tacccattgg ccttgctaag caagcgcagg
    24841 gacgtgcttg tctggcggtg atggtgagtt tcctgacctg gaactatttc atcaatgcga
    24901 tgggaatgac ctggggaagc tacttcggcg tcgatttcac tcaggacgcg gtggcaggta
    24961 gcggtctgac aatgatggcc gggattaaaa ccctcgatac cagcattatc ggcgcaatta
    25021 tcatttccgg cattgtgacg gcgctgcata accgtctgtt cgataaaaaa ctgccggttt
    25081 ttctcggcat tttccagggg acgtcttatg tggtgattat cgccttcctg gtgatgatcc
    25141 cctgtgcctg gctgacattg ctcggctggc caaaagtaca aatggggatt gaatctctgc
    25201 aagcattcct gcgttcggcg ggtgcgcttg gggtgtgggt ttacaccttc ctcgaacgta
    25261 ttctgatccc aaccggttta caccacttca tctacggacc gtttatcttt ggtccggcag
    25321 ctgttgaagg cggcattcag atgtactggg cgcagcatct gcaagagttc agtctgagcg
    25381 ccgagccact gaaatcgttg ttcctggaag gcggttttgc cctgcacggt aactcaaaaa
    25441 tctttggtgc cgtgggcatt tctttagcga tgtacttcac tgccgcaccg gaaaatcggg
    25501 taaaagtggc gggcttgctg attcccgcaa ccttaaccgc catgctggtg ggaattaccg
    25561 aaccgctgga atttaccttc ctgttcattt caccgttgct gtttgcggta cacgccgtgc
    25621 tggcggcctc aatgtcgacc gtaatgtatc tctttggtgt ggtgggcaac atgggcggag
    25681 gtctgattga ccaggtttta ccgcaaaact ggatcccgat gttcagcaac cacgcggata
    25741 tgatgctgac ccaaatcgcc attgggttgt gctttaccct gctgtacttc gtggttttcc
    25801 gcaccctgat tctgcagttc aacatgtgca cgccgggacg tgaagatgcg gaagtgaaac
    25861 tctactcaaa agccgaatac aaagcctcgc gaggccaaac caccgctgca gagccaaaaa
    25921 aagagctgga tcaggctgcc ggtatcctgc aagccctggg cggggtcggc aatatctcca
    25981 gcattaacaa ttgcgcgacg cgtctacgta ttgcactgca tgacatgtca caaacgctgg
    26041 atgacgaagt ctttaaaaag ctgggagcgc acggcgtctt ccgtagtggc gatgccattc
    26101 aggtgatcat tggtctgcat gtatcccaat tgcgtgaaca gctcgatagc ttaattaatt
    26161 ctcatcaatc agcagaaaat gttgccatta cggaggcagt ataatgacca aattctcagt
    26221 ggttgtcgca ggcggtggaa gcacctttac gccaggcatc gtgttgatgc tcctggcgaa
    26281 tcaggaccgt ttcccgcttc gtgcactgaa attttatgat aacgatggcg cgcggcagga
    26341 agtgattgcc gaagcctgta aagtcatcct taaagaaaaa gcgccggaca ttgcgtttag
    26401 ttacaccacc gatcctgaag tggcgttcag cgacgttgat tttgtcatgg cgcacatccg
    26461 cgtcggcaag tacccgatgc gcgaactgga tgaaaaaatc ccactgcgcc acggtgttgt
    26521 tggtcaggaa acttgcggac ccggcggaat agcgtacggc atgcgttcca ttggcggcgt
    26581 cctggaactg gtggattata tggaaaaata ttcaccaaac gcctagatgc tcaactactc
    26641 caacccggca gcaattgtcg cagaagccac gcgtcgtctg cgcccgaatg cgaaaatcct
    26701 caacatctgt gacatgccaa tcggtattga aagccggatg gcgcaaattg ttgggctgca
    26761 agatcgcaaa cagatgcgcg tgcgctacta cggcctgaac cactggtggt cggcgatatc
    26821 ccgcagtttc agaaagggtt aatgagtcag caagtggcgg tggaaaaact ggtcgtggac
    26881 gcctgggaac agcgttcata tcagcacctg tggcaggcga ttacgttgtc gaaaacggta
    26941 ccgagcgcct cggtcgccaa agctattctg gatgagttgc tggaggccaa caaagcgtac
    27001 tggccagagt tacgttaatt gtgtggactg gcatccgttg ggtgctggtc cgtattcttt
    27061 agcacggctg acggcgcata accaaattta tgtttaaagg ctttgctgaa atgaaaggag
    27121 tcgaagaagt tgagcgtatc agcgacttcg ccaaccgagt ttccttgttg ctttaatagc
    27181 gatagcgcca gatccagacg cgcatcgagg taatattctt tcggcgtctt gccggtatag
    27241 cgtaaaaaca gacgacgcaa ccaggcttcg ctacagggga tcgtggcagc catatcagct
    27301 acgctccagc gttgttgcag actggcatgt aaagtggcaa ttaatttttc aatttgccgc
    27361 cgttgtggat cttttttacc atctgcggac ataagacaaa tccactgata aataattttc
    27421 gtcagaaaag cgactgccag attatttttt attgcttctg gtgaagttat taactcagca
    27481 acttcggtga gttcctgatt ataaatttcg ccattataaa taacgctttg ctgaccaacg
    27541 gaaatatcca tcatactggt gggggtaaat tccatccagt actgttccca gactaaacct
    27601 tcacagtgat aagagtgaat atccattggc tttaaaaata taatacagtt gctatgcaga
    27661 gttatttgtt cgccattctt taaaagaatt ttcccacatc cttgtacggt atacaccgca
    27721 acccatgagt tggcgataag cggtttcttc ttgtcccgcg gccagatcac ccggtaatgc
    27781 tcgtcggcgt aggtatgcca gagcgagatc agagaaattc cgctggagat cacattttcg
    27841 ttaatcaata agggaatatt gtacggagtt tcgtttttta catctgaact ttgcaatttt
    27901 ccattcattt taatatccct ccggcgaatt atgctcacaa cctgaactta acagaaaaca
    27961 atccgttcag ggatacgacg tttgtatttt aagaatctga ctgcctgacc cgacgcattt
    28021 tacctctccc tatattcatg cgtccgggac ataaacataa ataagggcta tgagatgaat
    28081 tcgttacaaa tcttgagttt tgtcggtttt acgctgctgg tggcggtgat cacctggtgg
    28141 aaggttcgca aaacagatac cggatcgcaa caaggctatt ttcttgccgg acgttcacta
    28201 aaagcgccgg ttattgccgc ttcgttaatg ttaaccaacc tttccacgga acaactggtt
    28261 ggtctttccg ggcaggccta caaaagcggc atgtcggtga tgggctggga agtgacttca
    28321 gcggtgacgc tgatcttcct cgcgctaatc tttttaccgc gctatctgaa gcgcggcatt
    28381 gccaccatcc ccgattttct ggaggaacgt tatgataaaa cgacgcgtat tatcatcgac
    28441 ttctgcttcc tcattgccac cggcgtctgc tttctgccga ttgttctcta ctccggcgcg
    28501 ttggcgctca acagcctgtt tcacgtcggg gaatcgctac agatttctca cggtgcggct
    28561 atctggctac tggtaatttt gcttggtctg gcgggaattt tgtatgcggt gatcggcgga
    28621 ctgcgcgcaa tggcagtggc ggactccatc aacggtattg ggctggttat cggcgggttg
    28681 atggtgccgg tatttggcct gatcgcgata ggcaagggca gctttatgca gggcattgag
    28741 caaattacca ccgttcacgc cgagaaatta aactcagtcg gtggcccgac cgatcccttg
    28801 ccgattggcg cggcatttac cggtttaatt ctggtgaaca ccttttactg gtgtacaaat
    28861 cagggcatcg tgcaacgcac gctggcgtca aaaagcctgg cggaagggca aaagggggcg
    28921 ctgttaacgg cggtgctgaa aatgctcgac ccgctggtac tggtgctgcc agggttgatt
    28981 gcgtttcatc tgtatcagga tctaccgaaa gccgacatgg cctacccgac gctggtcaat
    29041 aacgttctgc cagtgccact ggagggtttc ttcggcgcgg tgttatttgg tgcggtgatc
    29101 agtaccttca acggctttct gaatagcgcc agtacattat tcagtatggg tatttaccgt
    29161 cgcatcatta accagaatgc cgagccgcag cagctggtca ccgtcgggcg caaatttggt
    29221 ttctttatcg ctatcgtttc ggttttggta gcgccgtgga tcgcgaacgc gccgcagggg
    29281 ctgtatagct ggatgaaaca gctcaacggc atttacaacg tgccgctggt taccatcatc
    29341 attatgggct ttttctttcc gcgcatcccg gcgctggcgg caaaagtggc gatggggatt
    29401 ggcataatca gctacatcac catcaactat ctggtgaagt tcgacttcca tttcctctat
    29461 gtgctggcct gtacgttctg catcaacgtg gtcgtgatgc tggtgatcgg ttttatcaaa
    29521 ccgcgcgcca cgccgttcac cttcaaagat gcgtttgcgg tggacatgaa accgtggaaa
    29581 aacgtcaaga tcgcgtcaat tggtatcctg ttcgcgatga ttggcgtcta tgtcgggctg
    29641 gctgaattcg gcggctacgg tacgcgctgg ttagcgatga tcagttattt catcgctgcc
    29701 gtagtgattg tctacctgat ttttgacagc tggtggcatc gtcacgaccc agccgtaacc
    29761 tttactcccg acgcgaagga tagcctatga aacgccccaa ttgaccgata cccaggccac
    29821 caatatggtc ggttgctata gcggtaaacc gctgaatacg caaaatattg atagtctggc
    29881 ggcggaaggt attcgcttta attccgccta cacttgttca ccggtttgta cgcctgcacg
    29941 ggccggacta ttcaccggta tctacgctaa ccagtccggc ccgtggacca acaacgtcgc
    30001 gccaggcaaa aacatctcca ctatggggcg ctactttaag gatgccggct atcacacctg
    30061 ttacatcggc aaatggcatc tcgacggtca tgactatttc ggcactggcg agtgtccgcc
    30121 ggagtgggat gctgattact ggtttgatgg ggcgaactat cttagcgaac tgacggaaaa
    30181 agagatcagc ctgtggcgca atggcctaaa cagcgtcgaa gatttacagg cgaaccatat
    30241 tgacgaaacc ttcacctggg cgcaccgcat cagcaatcga gcggtagatt ttctgcaata
    30301 gcccgtgcgc gccgaggaac ccttcctgat ggtggtttcg tatgatgagc cgcatcaccc
    30361 gttcacctgt ccggtggagt atttagagaa atacgctgat ttttactacg aacttggcga
    30421 gaaatcacag gatgacctgg cgaacaaacc ggaacatcac cgcttatggg cgcaggcgat
    30481 gccatcgcca gtcggtgatg acgggcttta tcaccatccg ctctattttg cctgcaatga
    30541 ctttgttgat gaccaaatcg gacgtgtcat caatgcctta acgccagagc aacgtgaaaa
    30601 tacgtgggtc atttatacct ccgatcacgg tgaaatgatg ggcgcacata agctgatcag
    30661 taaaggagcg gcgatgtatg acgacatcac ccgtattccg ctgatcatcc gttcgccgca
    30721 aggggagcgg cgacaggtcg atacgccagt cagtcatatt gatttactgc cgacaatgat
    30781 ggcgctggca gatattgaaa aaccagagat tctgccgggg gaaaatatcc ttgccgtgaa
    30841 agagccacgc ggcgtgatgg tggaatttaa ccgctacgag attgagcatg acagctttgg
    30901 cggttttatt ccggtgcgtt gctgggtgac ggatgacttt aaactggtgc tcaacctctt
    30961 caccagtgat gaactttacg atcgccgtaa tgacccaaat gaaatgcata acctgatcga
    31021 tgatatccgt tttgcagacg ttcgcagcaa aatgcatgac gccttattgg attacatgga
    31081 caaaattcgc gatccgttcc gcagttacca atggagtctg cgtccgtggc gtaaagatgc
    31141 acgaccgcgc tggatggggg cgtttcgccc acgcccacaa gatggctatt cgccagtggt
    31201 acgcgactat gacaccggcc taccgacgca aggagtgaag gtggaggaga aaaaacagaa
    31261 attctgacga taatgcccgc tgatgatcac ccggcgggca ttattcaggc tgcttaaacc
    31321 agcgaaccgc cggtcaggaa ggaaaaaatc aggaatatca gcaaaatgct gcacacgatg
    31381 ccgataccaa aaaccagcgc gccgcgaatg ccccagcgat tttccttacg cataccgcca
    31441 ataaatgcgc aaagtagcgc agcaatggcc agtgcgatac cgctttgatc gaggatttta
    31501 tcgatatcca tgtcctcttt tttctccaca gtggtaattt tttcgccttt tattccggcg
    31561 agaagacctc ttttaatttc agccgttttg tccgccacga tgctttcaag cgttggcggt
    31621 ggtgcggaaa aagggccgaa tgaaaagtgg ataatcccca tcattaaagc gatggcaccg
    31681 aacagcatgc ccaatgaact gattttattt tgcgctatga tacccatctg acctccttgt
    31741 agcggatggc aaaagcatat caggaaacgg gcggacgaaa ttcttaaaaa aaaccgctaa
    31801 agatagagcg ttagtgaaat tgcaaaggag caacaatgaa aatttcccgc cttggagaag
    31861 cgcccgatta ccgcttctcg ctggctaatg agcgtacttt tctggcgtgg atccgtaccg
    31921 cactaggatt tctggcggcg ggggttgggc ttgatcaact tgcgccagat ttcgccacgc
    31981 ccgtcattcg tgaactgctg gcgttgttgt tgtgtctgtt ttccggcggg ttagcaatgt
    32041 atggctacct gcgctggtta cgcaatgaaa aagcgatgcg tctgaaagag gacctgccct
    32101 acaccaacag cttattaatc atcagcttaa tattgatggt tgtggcggtg atcgttatgg
    32161 gactggtgtt gtatgccgga tagccgtaaa gccagacgca ttgccgaccc agggctgcag
    32221 ccggagcgca catcactggc gtggtttcgt accatgctgg gctacggcgc gttgatggcg
    32281 ttggctatca aacacaactg gcaccaggcg ggcatgttat tctggatttc gattggcatc
    32341 ctcgccatcg tggcgctgat cctctggcac tacacccgta atcgcaattt aatggatgtc
    32401 acgaatagcg atttttccca atttcacgta gtccgtgaca aatttttgat ctccctcgcg
    32461 gtgttatctc tcgcaatact gtttgctgta acgcatatac atcaacttat cgtatttatt
    32521 gagagagtcg catgacagga agtcaggtca ttgatgctga agaggacagg cataagctgg
    32581 ttgtagagta caaggatgct ctgcaacctg ccgattttta tcacaatttc aaacagcggg
    32641 gcatccgctc cgtacagctc attccctatc ttgaatttga tgaccggggc gatctgacgg
    32701 ccgcttcggt gacagcagag ctgtggggga aatttttaat tgccctgttt gagtgttggg
    32761 tgcgggcaga tatcagtcgt atctcgattg agctttttga cgccacctta caaaaatggt
    32821 gtggcagcga aaacccgcaa ccgcgccgtg actgccaggc gtgcgactgg catcgcctgt
    32881 gcccgcacgc tcggcaggag acgccagaca gcgtgctttg tgcgggctat caggcgtttt
    32941 attcttactc cgcaccgcat atgcgggtga tgcgtgatct gataaagcag catcgttctc
    33001 ccatggagct gatgacgatg ctgcggtgac gtgcgcttaa acgggctgcc cctgatgcga
    33061 catccgcgtc gccagcggca gccagcacag cacgatcaac aatcccatta aggtcatcaa
    33121 caaccccagg ctgccctgac cggtttgcgg caacatcgca gagagcgacg ccagcacgcc
    33181 ggaaccaatg ttttgcagac cgccgaccag cgcaccagcc gtgcccgcca ggaaggggaa
    33241 cggctccatc gcgccgctgg tcgccagcgg aaacagcatc ccggcaccga aaaagaacag
    33301 cgcggcggga acgagcagcg tccagacatt catcacgcca aaccagtcgg ggatccacat
    33361 cagcaagcca gccagcaggc agcagataac cgactgccac atcaacgttg agaagcgttt
    33421 attaggacgc ccggcaaacc atgccccaaa aaatgccgcc ggaatcggca gaataaacaa
    33481 aatactgacc gtcatactgc tcagccctaa caccgcgccc atcagcacgc cggagcaggc
    33541 ttcaaaggca gcaatcccgg ccagaccgcc aatcagcatc agcaaataac agttaaaacc
    33601 gctgttaccg aaaagcgttt tataactggt aagcaggcgc gtgcgcggtg catcgaccgg
    33661 acgcgtttcc ggcatccagc gggccatact gaaggtcaca ccagcacaaa gaaccaacaa
    33721 aaagagataa caggcgcgcc agttccacat cgtatccagc agaccgccga ttagcggtgc
    33781 gagcaacgga ctgacgagaa tccccatgtt taacaggctg ttagcatggc gcaactgtgt
    33841 ccgttcatat aaatcacgcg gcaaagtacg cgccattacg ccgccaacgc cggtgcccat
    33901 cccctgcatc gcgctggcgg caatcaatac cgtcaaactg gaggtcgtga ccgcgaccag
    33961 cgttgccagc ataaaaatgg acattccgac gaggatcact ggtcggcgac cgacacggtc
    34021 ggatatcggg ccaaaacagc tgtgagacac c
//
LOCUS       DAZXUK010000016        33531 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZXUK010000016 DAZXUK010000000
VERSION     DAZXUK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33531
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.016"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            376..815
                     /gene="ycgY"
                     /locus_tag="ACH3L8_000644"
                     /pseudo
     CDS             376..815
                     /gene="ycgY"
                     /locus_tag="ACH3L8_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
     gene            complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACH3L8_000645"
     CDS             complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACH3L8_000645"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFT4083889.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACH3L8_000646"
     CDS             complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACH3L8_000646"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFT4083890.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACH3L8_000647"
     CDS             complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACH3L8_000647"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFT4083891.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACH3L8_000648"
     CDS             complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACH3L8_000648"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFT4083892.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6281..8200
                     /gene="dhaR"
                     /locus_tag="ACH3L8_000649"
     CDS             6281..8200
                     /gene="dhaR"
                     /locus_tag="ACH3L8_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFT4083893.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(8300..11167)
                     /locus_tag="ACH3L8_000650"
     CDS             complement(8300..11167)
                     /locus_tag="ACH3L8_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFT4083894.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(12012..13103)
                     /gene="ychF"
                     /locus_tag="ACH3L8_000651"
     CDS             complement(12012..13103)
                     /gene="ychF"
                     /locus_tag="ACH3L8_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFT4083895.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(13220..13804)
                     /gene="pth"
                     /locus_tag="ACH3L8_000652"
     CDS             complement(13220..13804)
                     /gene="pth"
                     /locus_tag="ACH3L8_000652"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFT4083896.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            14082..14360
                     /gene="ychH"
                     /locus_tag="ACH3L8_000653"
     CDS             14082..14360
                     /gene="ychH"
                     /locus_tag="ACH3L8_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFT4083897.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(14415..16094)
                     /gene="dauA"
                     /locus_tag="ACH3L8_000654"
     CDS             complement(14415..16094)
                     /gene="dauA"
                     /locus_tag="ACH3L8_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFT4083898.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(16219..17166)
                     /gene="prs"
                     /locus_tag="ACH3L8_000655"
     CDS             complement(16219..17166)
                     /gene="prs"
                     /locus_tag="ACH3L8_000655"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFT4083899.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(17317..18168)
                     /gene="ispE"
                     /locus_tag="ACH3L8_000656"
     CDS             complement(17317..18168)
                     /gene="ispE"
                     /locus_tag="ACH3L8_000656"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFT4083900.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(18168..18791)
                     /gene="lolB"
                     /locus_tag="ACH3L8_000657"
     CDS             complement(18168..18791)
                     /gene="lolB"
                     /locus_tag="ACH3L8_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFT4083901.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            19005..20261
                     /gene="hemA"
                     /locus_tag="ACH3L8_000658"
     CDS             19005..20261
                     /gene="hemA"
                     /locus_tag="ACH3L8_000658"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFT4083902.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            20303..21385
                     /gene="prfA"
                     /locus_tag="ACH3L8_000659"
     CDS             20303..21385
                     /gene="prfA"
                     /locus_tag="ACH3L8_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFT4083903.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            21385..22218
                     /gene="prmC"
                     /locus_tag="ACH3L8_000660"
     CDS             21385..22218
                     /gene="prmC"
                     /locus_tag="ACH3L8_000660"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFT4083904.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            22215..22607
                     /gene="sirB2"
                     /locus_tag="ACH3L8_000661"
     CDS             22215..22607
                     /gene="sirB2"
                     /locus_tag="ACH3L8_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFT4083905.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            22611..23420
                     /gene="sirB1"
                     /locus_tag="ACH3L8_000662"
     CDS             22611..23420
                     /gene="sirB1"
                     /locus_tag="ACH3L8_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFT4083906.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            23456..24310
                     /gene="kdsA"
                     /locus_tag="ACH3L8_000663"
     CDS             23456..24310
                     /gene="kdsA"
                     /locus_tag="ACH3L8_000663"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFT4083907.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24459..24566)
                     /locus_tag="ACH3L8_000664"
     CDS             complement(24459..24566)
                     /locus_tag="ACH3L8_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFT4083908.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(24971..26071)
                     /gene="chaA"
                     /locus_tag="ACH3L8_000665"
     CDS             complement(24971..26071)
                     /gene="chaA"
                     /locus_tag="ACH3L8_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFT4083909.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            26341..26571
                     /gene="chaB"
                     /locus_tag="ACH3L8_000666"
     CDS             26341..26571
                     /gene="chaB"
                     /locus_tag="ACH3L8_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFT4083910.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            26729..27424
                     /gene="chaC"
                     /locus_tag="ACH3L8_000667"
     CDS             26729..27424
                     /gene="chaC"
                     /locus_tag="ACH3L8_000667"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFT4083911.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(27468..27821)
                     /gene="ychN"
                     /locus_tag="ACH3L8_000668"
     CDS             complement(27468..27821)
                     /gene="ychN"
                     /locus_tag="ACH3L8_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFT4083912.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            28006..29400
                     /gene="ychO"
                     /locus_tag="ACH3L8_000669"
                     /gene_synonym="iatA"
     CDS             28006..29400
                     /gene="ychO"
                     /locus_tag="ACH3L8_000669"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFT4083913.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(29401..30051)
                     /gene="narL"
                     /locus_tag="ACH3L8_000670"
     CDS             complement(29401..30051)
                     /gene="narL"
                     /locus_tag="ACH3L8_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFT4083914.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(30044..31840)
                     /gene="narX"
                     /locus_tag="ACH3L8_000671"
     CDS             complement(30044..31840)
                     /gene="narX"
                     /locus_tag="ACH3L8_000671"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFT4083915.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            32179..>33531
                     /gene="narK"
                     /locus_tag="ACH3L8_000672"
     CDS             32179..>33531
                     /gene="narK"
                     /locus_tag="ACH3L8_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFT4083916.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     V"
BASE COUNT         8232 a         8301 c         8552 g         8446 t
ORIGIN      
        1 cattttatta atgcatttct cattcgggtg aaatttccgc tacctggacg cataaaaccg
       61 gaactctcgc caaaagtcgg gcactccgaa ggaattgacg cgttgcgctg ctctgtaacg
      121 gcatttaccg caagccattt atttcgccag cgacaatccg cagagaacca aacgcatcag
      181 gaaacgactg aacacaataa atactgtatt gatcaacata ttatactggc acctccagca
      241 cgcagtgagg atacactgta tcatcagcga atcttatcac tgcggagctg gttgaacgac
      301 tttctgagcg aaataagtcg gttcccggta acaacaacag aatccttttt taattattgt
      361 ttcgttgttt ttggtgtgat tcagaaaata aatattttag acagtagcca gtcaggcgtt
      421 aaaaatgggg cggatagtga ggcggttttg gctgcgctta ttgaacattt agaattgatc
      481 aatccttctg gcagattaag ccaaaatacc cgttcggcaa tgcttcagtt acgcgaagag
      541 gaatggtctg aattcttttt ttggttgcta aattctctgg agtgtctgga ttatgtgatt
      601 attaatttaa ctccagaaag caaaaaaacg ttgatgagtg aacaccgtaa caatattcgg
      661 tagcaattga tgctttatat agccaacgtc ggcgtaaatc gccgggtgat gaaagtgaaa
      721 cacttacccg tagaaatgac gctatcttcg gcaaccacgt atggcaaaca tttgcgcaat
      781 acttcccgcc aggacttgaa aaaccgtctg tgtagaaaaa accatagata tggtgttatc
      841 tgccgacaac gccatccgga gaagccgaat gacgttgtcg gagtaagcgc tggttaaggt
      901 gtgggttgtg cctctttggt tgagggttgc gtcgttgctg acttaacggt cggatgcgtc
      961 gccggaacat tgtcacacgg ttgctctttc gggcagatca aatccagcat tttcagcgtc
     1021 acgccattgg tccagccaaa gccatcctgt aatggatatt cgccaccgcc gccccccgtt
     1081 ccggtggtgc tgacatcata tttttccacc agctttttct cccggtcata ggtgtgctga
     1141 acattggtca ggaagtgcca gctaatgtcc atcgccacct ctttttgccc gtagttttgt
     1201 aatccttctg tcgcgaccca ctgtaacggt gcccagccat ttggcgcatc ccattgttgc
     1261 ccacttttca ccgacgtggt gttcaggccg ccgggttgca gcagatgtgt tttcgtcgcc
     1321 gtcgccattt tgttggcgcg atctttcgct gccgcattga cgtacagcgg gaacagggcg
     1381 gccgcggtta actgattgcg cactttatga cttttcaggt cgtaatcggc ataccagcct
     1441 tgttgatcgt tccacaggta tttttcgatc cctttttgac gggcattcgc cagcgtttcg
     1501 tactggtttg ccatcgcgtt atctcctgca gctttgctgg cgcgggcgag gattttttcc
     1561 attttaaaca tcaggctgtt cagatcgacc ggtacgatgc tggtggtgcg taaggtattt
     1621 aactgctgcg ggttgtccat ccagcgcgag ctgaaatccc agccagacgc agcggcagag
     1681 cgcaggtcgc ggtaaatttc agtggcaggt cgattcggat tgcttttggc ggtggcaata
     1741 tcttccaccc atgactctgg tcgtggcgta tcgcgatcgt cccagtagcg gttgagaagg
     1801 gtactatcct gaagttttac aacgcgtttt tcctgttgtc cggcttgcag gttttcaaca
     1861 ccgtccatcc agtaagcata ttctttttgc atttgcggca ggtattgctt caacgcggca
     1921 tcgccttcat gctgcgccag taactctacc atcagggcaa agaagggcgg ttgcgagcgg
     1981 cttaaatagt aactgcggtt gccgttggga atatgaccgt aagtgtttat ttcatgagca
     2041 aaattggcca ccatatccgc gattttatcc cagtgaccac tttcggcaag tcctaacatg
     2101 gtgaagtaat tgtcccagta atatacctcg cgaaaacgtc cgcccggcac gacataaggt
     2161 tccggcagcg gtaacagaga atcccatttt tcggtgtttt cggtagaacg cgttaatacc
     2221 ggccaaagtc cgtcaatatg ttcgcgcagt gactgcccct ctggcggaac atatttctcg
     2281 ccttctttcg gcagggtgaa attgacgtta acgaaatggc gcagatcaaa tccgctctgg
     2341 ttttgctgca tccgataatc agcaaggatc atcagcggat cgctgttagg cacggcatcg
     2401 gcaaaggttt tttggtccgg aaaaagtttg acgttttgca catcattaaa cagcggccct
     2461 aataaaatat caggcggctg tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca
     2521 gcgaaacaca aaaagataca ggctggaatt aacgccattt tttgcgggcg agaaggtgcg
     2581 ggggatttca tcaatcattc tcctttggcg aaaccgaata aaacggtatt caagtatcag
     2641 aaaaccttag ttcaggatcg cactagctgc atgatcgaaa tctcattctg cgaacggtca
     2701 ggcattttag aaaagggaat taaagggata aataacggcg aaatagagtg atcaaataac
     2761 gtctgtttat tagccagcca gcgacatttg gttcgcgatt agcgaagaaa aatcatcata
     2821 aattggtaaa aaatataaca ggtagttaaa aaacatcagt gctgggtaaa ttgccggatg
     2881 acatcagaac gatgccatcc gaacggtggc ttaaccctga cggttgaaac gttgcgtttt
     2941 aacgtccagc gttagcgttt cttctggttg tatcgcatac agtttctcac cctgctggca
     3001 aatccagcca atccccagtt cacgggcgat tagggcgctg tgggataccg gacttccggc
     3061 gctaaggcag atacctttta caaccgccgg atccagttgc agtactgtgg aaggataaat
     3121 gttctccgcc agtagaatag ttggcgagtt aaactgcggg agttcttctt tcgtttgggt
     3181 caggtggacc agggtgcgat gcagaagatc gtccacatca atatagcgag cttgtagata
     3241 ttcatcatcc agttgctggt attgctggct aagttcttta agaacttgct gccaggcata
     3301 ttctgccgtg caatgttcat gctgaaggag ttcgcttgcc gccgccagca gttccggatc
     3361 acctaacagt gtatggtgac cagaaaagat tgcggcaata tcgtcaagcc cgctggcttc
     3421 tgctttcgct gttaacgtca tcagatctaa taacgtgaag tcaatagcct ggcgtaatcg
     3481 atcttgttct tcttccgcgg tcagggttga ttttgcctgt accgtacata aaactggttg
     3541 ataataaaag gctttacccg aaacaggcag aacgggacgc agagtaggtg gagcgacttc
     3601 ctccgtttca ccaaagttat cttcagccag gtgacggaaa gcgatcagtg cctcttcagc
     3661 ttctggccct ttcgcaatca ggcgcagcgt atcgttatag cgaacttgta gtaacgcaat
     3721 ctggttaata ctctctggtg tgacgcattt gccgtttttt tccagcaaca tatcggcatt
     3781 aaatgtcgat aaggtataaa ccagccggga ggccggacgt acatgcaggc cgttacggtt
     3841 ttttatgacc accgccagag aacgggcttc ttcatcgtac ggaggacatg tgtcagagat
     3901 ttcagtgtcg gaggacggta aacccagttg ttcacgtttg gcttccagcg catgcatagc
     3961 gtcaaagata actttgtcga tatccgcccc cgaggccgcg ctgaccgttg ctgccagtgt
     4021 accttcgacc aacggcgcag cacacaaacg tacttttgcg gcgatctcgg gagccagcaa
     4081 ttccagcgca gtttcagcac tcaataatgc gctacccata tccatcatga ccagcacatg
     4141 gtcggcatca gcaacagatt cgatggcctc catcactttg acggcatcgg taccaatggg
     4201 attttgtgga tcgtcaattc ccgcggcaat ggcgatttta caactatcac tcattaacat
     4261 ctgacgggct aattcaccga caccttctcc cagtcggctg ctatgtgaaa ctatgaccag
     4321 gtttaccatc accaattcct tactcttttg cggctaacgc caacatttgc atcataaaca
     4381 tcaccgaggt cgcgccggga tcctggtgac caatactgcg ttcaccgaga taactggcgc
     4441 ggcctttgcg ggcttgcatc gtaatcgtac tttgtgcagc ggattcggcg atgctactgg
     4501 cagcttcgag cgccaccgga acagagagat tttgctcgct ggactgacgt aacgattcca
     4561 ccaccggcac ccacacatca cacatggttt tatcgccagg ttcggctttc ccgcgactga
     4621 ttacgccgtc cgcgccatcg cggaacatct gataaagctc ttccagtgtc aggctttgcc
     4681 gtgcctgggt cgcctgtgcg gcgcggataa agaaggtacc gaacagcgga ccactggcac
     4741 caccgacgct ggaaagcagc gtcataccgg tatttttgag aatgaaaccg atatctttat
     4801 ctgcgatagc agggagtttt tccaccactt tgctaaagcc tcggttcata tttagcccgt
     4861 ggtcagcgtc gccaatctcg cgatccagcc cggtaagata ctcgctctcg gtgttgaaaa
     4921 tatcgccaca acgagtgagc cagttaacaa tttgagttct gctcagtgac attgctttct
     4981 ccttatttac cccagttaag ggccggggtg tggaccgggg cgtcccagag agccagcgtt
     5041 tcgtcatcaa ctttcagtaa ggtgattgag aaaccggtca tatccagtga ggtgcagtac
     5101 gcgccaatta aattacgttc gatagtcaat cccgcttgct ggcaacgtgt ggtcaggcgg
     5161 ttatagacgc cgtacagctc agaaagcgga gttgcgccaa gattgttaac cagtgcaatc
     5221 acccgatcgc cagactggag cggttgtttg gtttgtggtt cttcctgcca gctgccttgt
     5281 tgataatccc agaaacgcaa agtgcgatgg tatgagccat gttccagcag ggtgtcgaac
     5341 atttcatcga cggtttgatc aagggaagag aaagggcggc ggtcaatacc tggctcacca
     5401 tgaatgccta cgccaaactc catctcatta tccgcaaggg taaaagaagg tttgcccgcg
     5461 gcaggaacgg tacaggcacc gagagcgata cctattgagt ggccttgatt attcaactta
     5521 cgccccagtt ccgcacaggc gtccagtgag tcgccacgct ccgccgctgc gcctacgagt
     5581 ttttcaatta atacggtgtt ggcaacgccg cgtcgcccgg cggtataaag actgtctttt
     5641 accgcaacgt cgtcatcaat gaccacagtg gtcactttta cgccgctatc gtgcagtaac
     5701 tcggtcgctg tttcaaagtt aagaatatcg ccggtgtaat ttttgataat caacagtaca
     5761 ccttcgccgc catcaatttg catggagcat tcaaagattt tatcgggcgt cggtgaggtg
     5821 aaaatttcgc ccggacaggc ccccgaaagc atcccctgac cgatataacc acagtgcatc
     5881 ggctcgtgtc cgctgccgcc acccgacagc agggcgactt ttcctgcaac aggggcatca
     5941 gctcgggtga catacaccgg atcctgatgc agtgtcagcg atggatgcgc tttcgccagt
     6001 cctgccagtt gttcgtccag tacgtcttgc acatcattga tcaatttttt cattattttg
     6061 ctccagcaat tacggtaggg catggatgat gttcaacgac acggcgacca ctgactgccg
     6121 atgaatccat tgtgcatcag gcaagggaaa agaaaattag cccaaattat gtttcatagt
     6181 gaaacatatg ctttaatgaa tgttccatat tgaaactttt acgtgtatta atacttaaaa
     6241 ttgcgagccg gaacaccttt tgtcataagg gatgcgggat atgagtggcg cttttaacaa
     6301 cgatggtcgg ggcatatctc ccttaattgc aacctcctgg gagcgatgca ataagctgat
     6361 gaaacgggag acatggaacg taccacatca ggcccagggc gtgacatttg cttctattta
     6421 tcggcgtaag aaagcgatgc tgacgctcgg gcaggctgcg ctggaagatg cctgggaata
     6481 tatggcaccg cgagagtgtg cgctgtttat cctcgatgaa accgcctgca ttctcagccg
     6541 taatggcgat ccgcaaacct tgcagcagct aagtgcactg ggattcaatg acggcacgta
     6601 ttgcgccgag ggaattattg gtacttgtgc gctatcgtta gcggctatct ctggtcaggc
     6661 cgtgaaaacg atggccgatc aacatttcaa acaggcactc tggaactggg ccttttgtgc
     6721 aacgccgttg tttgacagca agggccgatt gacgggaaca atagcgctgg cgtgtccggt
     6781 tgagcaaact accgcagctg atttgccgtt gacgttggca atcgcccgcg aggtcggtaa
     6841 tttactgctg acggacagtt tgctcgctga aactaaccgt catttaaatc aacttaatgc
     6901 cctgttagaa agtatggatg atggcgtgat tagctgggac gagcagggta atttgcaatt
     6961 tattaatgcc caggcggcgc gggtcttgcg ccttgacgcg acggcaagtc agggacgggc
     7021 aatcactgaa ctcttaacgt tacccgccgt attgcaacaa gcaataaaac tggcacatcc
     7081 gctcaaacac gtagaagcaa cctttgaaag ccagcaccag tttattgatg cggtgataac
     7141 ccttaaaccg ataatagaaa cgcagggaac cagctttatt ttgttgctcc atcctgtgga
     7201 acagatgcgg cagttaatga ccagtcaatt aggaaaagtc agccatacct tcgctcatat
     7261 gccacaggac gatccgcaaa cccgccgctt gattcatttt ggtcgccagg cggcgcgcag
     7321 tagctttcct gtcctgcttt gtggagaaga gggcgtgggc aaggcactgc taagtcaggc
     7381 aattcataat gaaagcgagc gtgctgcagg tccttatatc gccgtcaatt gtgagttata
     7441 tggtgatgct gcgctggcgg aagaatttat tggtggcgat cgcacggaca atgaaaatgg
     7501 ccgtctgagt cggctggaac tggcacacgg cggcacgctg tttcttgaaa agattgaata
     7561 tctggcggtg gagttacagt ctgctttgct gcaggttatc aagcaggggg ttatcacgcg
     7621 actggatgcg cggcgtttaa taccaattga tgtcaaagtg attgcaacaa cgaccgcgga
     7681 cctcgcaatg ctggtggaac aaaatcgttt tagtcgccag ctgtattacg cgctgcatgc
     7741 atttgaaatt accatcccgc ctctgcgtat gcggcgtggc agcattccgg cgctggtgaa
     7801 taacaaatta cgcagtcttg aaaaacgctt ctctacgcgg ctgaaaattg atgacgatgc
     7861 cctcgctcgc ctagtttctt gtgcatggcc aggcaacgat tttgaacttt acagcgtcat
     7921 cgagaatctt gctctgagta gtgataacgg gcgcattcgc gtcagtgatt tacctgaaca
     7981 tctgtttacc gagcaggcga cagatgatgt cagcgccacc cgcctttcca ccagtctgtc
     8041 atttgcggaa gttgaaaaag aggcaattat taacgcagcc caggtcacag gcggtcgcat
     8101 tcaggaaatg tcggctttac ttgggatcgg ccgcactacg ctgtggcgga aaatgaagca
     8161 acatggcatt gatgcagggc agtttaagcg ccgggtatga aagacagaaa cgatttctga
     8221 tacatcagag tgatctgtat ttcattccgg cgcacgctaa caattttcag catcgtttaa
     8281 gggcttgtct atcccgcact taaaagctga agcgatatcc tccgttgact tgcttctgat
     8341 caaagaggtt accctgcgtg taatccgctt cgagatagaa tgtgtgctgt ttgttatact
     8401 gtgcactgac tcccacgccg ttattccagc cattaccttt gaaactgtac ttctcccggg
     8461 agtcgttcaa cagatattcg gtatcccctg aaaactcgcg gatagcgcca gtcttcacat
     8521 agacattcag ctggctgttg cctgcggtga tgtcataccc cagtatcatg ctggcacgcc
     8581 ccagcagcga ttcgtagtga ttcagatgta tattgaggcc attactcgcc ttcatagcca
     8641 tctcattctg gtggctgtat gtaagctggg tttgcggctc tatatagaac ccataaccag
     8701 taggggacag gttgaacctc tgcccggctt ccagggagat gctcattcca ttcgcagtgc
     8761 cgttggcgtt aacgccgttg ttctgactgt ccagtacgtg gaaactattt ttctggcgcg
     8821 atgcttttat aacgagatcg ctgtaaaaac cgttttgtgc catgtaactg gcgtacattc
     8881 ccatgtagtc tgaacgtgcg gtaccgtcgc ctccgctata gtccggcgat gcatgtgttg
     8941 agtcaatata cagaccgaca tacaacggca ttacatcaga gagacgttta tccccaccaa
     9001 actggatacc gctgtaaccc atgtcaaagc cgctcagttt gccactggca aaggagtcca
     9061 ggcttccccc ataactgcgc aaccagatat taccgtcttt actctgattt cgcaagtcac
     9121 ccatccgttg catcagcgta cggttttcaa catagttcaa taagtaaccg acattgagat
     9181 aattgccgcc agcatctgcg gtcgtggtgg gtttaggcgt gggagcgggt tcaggcgtag
     9241 gatcggggtt gactatggga ggctgagcgg gagctggtgt gggttcagga ttaggagtcg
     9301 gttccggaac tgtcccggaa gcgtaaagct cccagttagt gccattttta cgcacatcgt
     9361 acagatatcc ccccaactca acctgagaag acgcgctgaa cgaggccgcg ccgtcagtgg
     9421 tttttaccac tgtcagaact tcatttcccg ttgtggcctc gctgccctgg ttgcggatag
     9481 ccaatacgtg attaccagca ctactcccgc tgatattcaa taaatcccct ctattattaa
     9541 cgccattacc ctcgccaaca acatcagcac gcataataaa ggtactgtta ccgctcaggt
     9601 tctctacgtt taatgtggta aatgtgccgg cagttgaccc gtggctggca aaatcgacag
     9661 ttgaatggct cagcgccagc gtgtcgagat tagaattact tgttacgttc cagacgctgt
     9721 tattcattgc aacgtccagt ttcccgccat tgacattatc gctgagggag gaacctgtcc
     9781 aaaccgaccc agggtgcata tccagattga ttagcccacc tttggaaaga acgctaccgt
     9841 tgataagcat acgacccgag gcgttgatgc ggctggcggc ataaccatcg tcatgttgcg
     9901 ttgcgatggc catctggtcg ggtgtactca tatcaatgac cagatcgctc gtgaggtcga
     9961 tctggctgtt ggtcatggca taaatccctc tggctcctgc cgcgccggtg atagccaaac
    10021 tgtctccggt tattctaccg ccgctgagcg cccacaaacc cagcgccaga ctgccattac
    10081 gatcaaccgt aatatcggta ttttgcatgt tgataacagc cgttgccgtc tgggccgagg
    10141 caccataaga accgccggaa aagatgctgt ttcgttgcgc tgccgtgcca gaaaaattga
    10201 ttgtcgcgtc tgaactactg gtgacgaggc caccgccctg cgcggaagaa atatggctat
    10261 ctgcaccgat agtggttgta ccaccacgaa cttcgacgcc attggccgcg gctccagtta
    10321 catcaacagt gagtgcattc gcgctaacct ggccaaagct ccagaggccg tgagcattat
    10381 cgccattggt tttaatggta ctgtttgttc cgagatcgac aacagagttt ttctgtacgt
    10441 ttatccccat ggcgctgtag ccctgaacat cgattgtcag gtctgtcgcc gtaaaacgcg
    10501 cagcaccatt ggcgttattg ccgttgagac caccgatata aacacctgta cttccatcgg
    10561 tcttgatttt acttccgctt ccaagatcga cactggtgcc atagtcattg atggttaggc
    10621 ctataccgtt cgagttttca atggtgaatt gagtggctgt tagtgttgag ctatgcccaa
    10681 taatgatgcc gtcatcgtta ctcttaatgg tgctgcctgt gcctaagtca gcatgggtat
    10741 agtcaccaat taagttaatg ccgatggcag aggtttgacc aacaacatct actgtgagtc
    10801 ggttagctgt cagggagctg tttttgccct gaataatgat tccttttgca tatccagagg
    10861 cgtcgttgac attgacggtg acatcattac ccaggttgat attgccaggc gtgtttcctg
    10921 ctggggtcat tacaccatag aggtttcctg atggatcggc ggtatctcct gtaattctgt
    10981 caccatcatt aatagtaata ttggtgccat attgacttcc tgtatcagca gctatcagca
    11041 ggggggaaaa gataattgca ttcaaaccaa tagcgccaaa ttttatcagt tgaattgagg
    11101 gcgaacggat ttttaattca gcgagacgat cggctttggt attcccattg tgttgtttga
    11161 tgcccatacc actcctatat agtacccagg ctatgggtct atggattttg tcattttgtt
    11221 taagtcaatg aataatatcc tcttatcatc atgatatctg tatggtgcaa tcatgatgag
    11281 cttctctgag aataattctc tattaccggg caaaatcagg aacacaggaa ttaatatata
    11341 tttaaccccc ccccttacaa aggtgagttt atatcgagtt attccctaag ttgtcgttac
    11401 ttattccaat tcgttactat gaatatggca aaaatattac aacaaacagc ggttaaaaaa
    11461 tttggtgaag attttttgta ataatcctaa ttagttatgg gattttcgct tgatgtatct
    11521 gcataacaaa aacaatatgc gccatttttg cattactaat aagaaaaagc aaaaaacatc
    11581 gatattcatt tttgttcaat ggattttctg gcctatcgtt attattgtgc tactggttat
    11641 cattttcatc ttgccaggat cgcacgtaat cacgttattc ggggacttta tctgcgggat
    11701 attacccttc aggtaagtcc ttttctcgcc agcatattgg agcgagatca atattgatca
    11761 gctgtgaaga gggctgatgt gtagtggaac aaaaactgac gttatatcac tgctaatgag
    11821 atatcagtca tctgaacact tatgtcttta attattctcg tggttcactg taggcaataa
    11881 gcacaaaagt gtaggatgtt acaagaatga ttaggactcg gtgaaatgaa aaatccacgc
    11941 aattgcgtgg attttatata cttttgcgct cttcatgaga tttagcgaaa cctcatgaga
    12001 caataaatta attagacgtt gaaaaggaag ttcatcacat cgccatcttt cacgatgtaa
    12061 tctttacctt ctgcacgcat tttgcctgct tctttcgcgc cttgttcacc tttgtaagta
    12121 atgaaatctt caaacgagat ggtttgtgca cggataaagc ctttttcaaa atcagtatgg
    12181 attttgcccg ctgcctgcgg cgcggttgct ccaaccggaa tggtccatgc acgcacttct
    12241 ttcaccccag cggtgaagta agtttgcagg ttcagcagct tataaccggc acggatcaca
    12301 cggttcaggc ccggttcttc cagcccaagc tcctgcataa actcgtcacg ttcttcgtcg
    12361 tccagttcgg caatgtctgc ttcaacagca gcacaaaccg gaaccacaac agaaccttct
    12421 ttcgccgcga tttcacgcac ctggtcaaga tatgggttgt tttcaaaacc atcttcgttg
    12481 acgttggcta tgtacattgt tggtttcagc gtcaggaagc tcaggtaacg aatagccgct
    12541 ttctcttcag cgcttaaatc cagcgcgcgc agcatacctg cgttttccag ctggggcagg
    12601 catttttcca ggaccgccag ctcagctttc gcgtctttat cgccaccttt ggctttcttc
    12661 tgtacgcgat gaatcgcacg ttcgcaggtg tcgaggtctg ccagcgccag ttcggtgttg
    12721 ataacttcaa tatcgtcagc cgggttaact ttgccggaaa cgtgaatgat gttgtcattt
    12781 tcaaagcagc gaacaacgtg accgatcgct tcggtttcac ggatgttggt caggaactgg
    12841 ttacccagac cttcgccttt cgatgcgcct tttaccagac cggcgatatc gacaaattcc
    12901 atggtcgtgg gaagcgtacg ttgcggtttt acgatttcag ccagttgatc caggcgaggg
    12961 tcaggcattg gtacgacgcc tgtgttcggc tcaatggtgc agaatggaaa gttggccgct
    13021 tcaataccgg ctttggtcag cgcgttgaac agggtagatt tcccgacgtt gggcaaaccg
    13081 acgataccgc atttgaatcc catgatttaa atcaccttaa tatcttaata atcaacctgt
    13141 tattgctaac agattgcaga aatggaaata actttgccta ttatacacgg cactcggcaa
    13201 aaatgccgca gacaacgact tattgcgctt taaaggcgtg caatcggttc gttgctttgg
    13261 tcaagccatc tgtaaaccac atttcagtac aacgcgccgc ttcgtcgatg gcttcatcaa
    13321 ttaacttctg ttcactaaca ggcggtttgc ctaacacaaa accgacaact ttatttttat
    13381 cgcccggatg accgattccg atgcgtaaac ggtgaaagtt agggttatta cccaatttac
    13441 tgatgatgtc tttcagtcca ttgtgaccac catggccacc gcccaactta aatttggcga
    13501 cgccaggagg cagatccagt tcgtcgtggg ccaccagaat ttcgtccgga ttaatgcgga
    13561 aaaaactggc catcgccgca acggctttgc cgctgagatt cataaatgta gtcgggacta
    13621 acaggcggac atcttcgctt ccaagggtga ctcgcgaagt ataaccaaag aatttagcct
    13681 cttcgcgcag cggagcgcgc aaacgctctg ccagtaagtc aacgaaccag gcaccagcat
    13741 tatgtcgcgt tgcggcgtat tcagcaccgg ggttcgccag gccgacaatc aatttaatcg
    13801 tcacgttttt ttgtcctgag tgtgtacata actggcgcgt agtttactgg ttgcggcccc
    13861 gcttgacaaa aaactgcgta tcaaatgcag ataacgtaat aattgcctga gtggactatt
    13921 agaaagtcaa ggtgttcagg cgtttatttg taaagttttg ttgaaataag ggttgtaatt
    13981 gtgattacgc ccgcacataa cccacagggt gttgtctata ctttacacat aaggaagagg
    14041 ggtattccct gttacaaccc agaaagttcc ggaggtgaca tatgaaacgc aaaaacgctt
    14101 cgatactcgg taacgtgctc atggggttgg gtctggtggt aatggtggtc ggcgtggggt
    14161 attcaatcct caaccagtta ccacagttta atatgcccca gtatttcgca catggtgcag
    14221 tgctaagtat tttcgtcggt gccattctct ggctggcggg tgcccgtgtt ggcgggcatg
    14281 aacaggtgtg cgaccgttac tggtgggttc gtcactatga caaacgttgc cgccgtagcg
    14341 ataatcgccg tcatagctaa caaataatgc agtttgctga ccagtcagtt tgcgctgact
    14401 ggtcaatctc gtacttataa atccgccatc gccgcgcgac gattcgggaa gaacgcgagg
    14461 cgtcccggga tcggttgaat gcctgcgcga gccatagttc gcagtggctg gaattccacg
    14521 ttgcacacgc gcagttcaca tccttcgggc agacgcttca caaaacgctg gaacgcatca
    14581 agaccaccag catcaagtac cggaacggca tcccacttca gaatcacaat ccgtttgcct
    14641 tcaagacgtg actccaggtc cgtgaataag ccttcagcag cagcaaaaaa cagcgggcca
    14701 ataacgcgca gaaccaggac atcgtctgga acatctacga ctaccggtgc caggcgagtc
    14761 atacgtgcga tacgacgcat aaacagcagc gatgccagca cgatccccac gctgatggca
    14821 ataaccatat caaacagcac ggtcagtgac atacacagca gcatgacgat gatgtcatct
    14881 ttcggcgcat gacgcagcaa gtcgaccact ttgtgcgctt cactcatgtt ccacgccacc
    14941 atcaacagca gggctgccat ggcggaaagc ggcagccagg agagcagtgg tgccagtacc
    15001 agcagggcga gaataaccag aatagagtgg atcaccgccg agatagggga agttgccccg
    15061 gcacggacgt tagcggcaga acgcgcgatg gcagcagtag cggtaatacc accaaagaat
    15121 ggagcgatga tattccccag cccctgaccg accagttcac tattcgcctt gtgcttagtc
    15181 ccggtcatac catccagtac cacagcgcag agcagagatt cgatcgcccc gagcattgcc
    15241 attgagaatg ccgcaggcag cagggtgcga atagaatccc aggttagcgt gaattctgaa
    15301 ttaggcagat cccacggcag caccagttgc ggcagcagtt gcggaatacc gttaccctga
    15361 gaaccatcgg ccaggacgta gtggaattgc gaaccgatgg tagcaacatg tccgccgagc
    15421 aggttaacaa tccccatcac cgcgcaacca gccagcaagg ccggaaggtg accgggtaaa
    15481 cgaatgccca gacgcggcca gaaaaccaga atgccaagcg tcacaatgcc aatggcagca
    15541 tcacccacat taatggtcgg cagcgccata aataatgcgc cgactttttg tagataatgt
    15601 tccgggacat gggccatttg cagaccgaga aaatctttaa tctgcatggt accgatggtg
    15661 atcccgatac ccgaggtgaa acctaaggtg acggaaaccg gaatatactc aatcagacga
    15721 ccaaagcgtg ccagccccat cagaatcaaa aagattcccg acaacaaggt cgcaaccagc
    15781 agtcctgcca gtccaaactg ttgcgaaacg ggatagagaa ttaccacaaa tgccgcagtc
    15841 ggaccggaaa cgctaaagcg tgacccaccc gttagagcaa tgacaatccc cgcaacagct
    15901 gcggtatata aaccgtactg gggggccaca ccactaccaa tagccaacgc catcgccagc
    15961 gggatagcaa taatcccgac ggttatcccg gcaatcaggt cacgggtaaa ccgtgcggca
    16021 gtatattttt ctttccagca aacgtcgatc agagcgcgga aaggcatcac atgtgaggaa
    16081 aatattttgt tcacaataat gtttcatccg tgagcgcatc atctgtcaac taaatggcag
    16141 gtgaaggagg cataggtcat acaaatggat attacagaca aaaaaacccg ccgcagcggg
    16201 tctttgagcc gggttcgatt agtgttcgaa catggcagag atcgattctt cgttgctgat
    16261 acgacgaatc gcttcggcca gcatacctga cagggtcaga gtacgcacgt tcggcagtga
    16321 tttgatttca tcgctcagcg gaatggtatc gcagacaacg acttcatcaa ttacagagtt
    16381 acgcaggttg ttcgccgcgt tgccagagaa gatcgggtga gtcgcgtacg caaatacacg
    16441 tttagcacca cgttctttca gcgcttcagc agctttacac agcgtaccgc cagtgtcgat
    16501 catatcatcg accagtacgc agtcacgacc tgcaacgtca ccgatgatat gcatcacctg
    16561 ggaaacgttc gcacgcggac gacgtttgtc gatgattgcc atatcggtat cgttcagcag
    16621 cttagcgata gcgcgggcac gcacaacgcc gccgatgtcc ggagaaacca caattgggtt
    16681 atccagattc agctgcagca tgtcttccag caggatcggg ctaccaaata cgttatcaac
    16741 cggaacgtcg aagaaaccct gaatctgttc agcgtgcaga tccactgtca gcacacggtc
    16801 aacaccgacg ctggagagga agtctgcaac cactttcgca gtgattggta cacgagcgga
    16861 acggacgcga cggtcctggc gcgcatagcc aaagtagggg ataacagcgg tgatacgacc
    16921 tgcggaagca cgacgcaggg catcaaccat aacgactaat tccatcaggt tgtcgttagt
    16981 aggggcacaa gtggactgga tgatgaaaat atcaccaccg cgtacatttt cgttaatttg
    17041 tacgctgact tcgccgtcgc taaagcgacc tacagcggcg tcgccgagtg aagtgtacag
    17101 gcggttggca atacgttgtg ctagtaccgg ggtggcgtta ccagcaaaaa gcttcatatc
    17161 aggcacgaga agaacctcag gcatgcgtcc attggtggaa agaatctgcc gaaaactgtg
    17221 cgggccaggc atgatccttt ccaggcggtg tattaaagag cgcgatgcaa cgtctggaac
    17281 aaggtgacgt tgtcaccgaa actcagcttg cccggcttaa agcatggctc tgtgcaatgg
    17341 ggaaagatta acgcctttcg ccacaaagcc attgagccat tccggggctt gctctagcac
    17401 ctggcgggct tcagactctg tatcaaattc agcaaagaca caggcccctg tcccagtcag
    17461 gcgtgacggg gcgtattcta acagccagga aagcaccgca tcaacctcgc gaaaacgttt
    17521 tcttgcgata acctcgcaat cattgctgaa ttcacatttt agcaacgttt ctattgacct
    17581 ttttggcgta ttgcgcggga gttcaggatc tttaaaaatc accggagtcg gaatacttac
    17641 accagggtgc gccaccagat accacttctc tggcggatcc accggcgtta gtatttcacc
    17701 aacgccttcg gcaaacgcgg catgcccccg aacaaagaca ggaacatctg cgcccagcgt
    17761 cagccccatt tccgccagct catccatgct tagcccgcat tgccagagat gatttaatgc
    17821 caccaggacc gtcgcggcat tggatgaacc accgccgaga ccgccgccca tcggcaaacg
    17881 cttgtcaatg ctgatattcg caccgcttcc cgtcggaaga cgcccgctgt ctgccgcagt
    17941 tttcatcaac aaccgcgctg cgcgaacgat caggttatct tcatgttcca cgccttcaac
    18001 gggcgttaac agacgaatat ccccatcgtc acgaagctca atgctgatgg tgtcgccgta
    18061 atcaagaaac tgaaacagcg tttgcagcgt gtggtaacca tccgcacgct gaccggtaat
    18121 gtataaaaac agattaagtt ttgccggaga gggccactgt gtccgcatta tttcactatc
    18181 cagttatcca tttttaactt gatgcgttga ccaccgtcgg tgagttccat attggctggc
    18241 atcgcaggtt gcgttttggt gtcataacca ccataaacaa ccttccagtt tttgccattc
    18301 tggctgtagg taatttcgct caggcggtac tggtcgtcca gtttgtagtc ggttgcatca
    18361 cccggtaaac ctaaaatcca ctggcgcaag ctgttgagcg gaattggcat tccggtcaat
    18421 ttgccaatca tctcttcggc gtcatcggcg gtataacgct gacctttatt gtcgactaac
    18481 tgcacgttac ccggttgagc attcagctcc agttccgtgc tgcccaatgg gttagtgagc
    18541 agcagacggt agcgatcctg gccggtttgc tgccagaaaa agcgggcgta cactttttgt
    18601 tggtcagaaa tataagcgaa cgcgccgcga gtctgatact gattaagatt gcgcacgtct
    18661 tgctgatgct gacgccattg tggcgaatcc gggcttttgc caggaccttt gggcgtggta
    18721 acggaacagg cagtgagcac aagagcagcc agcggtagca ggcggataag acgaaaatcg
    18781 ggcaggggca tagtgatgac aagtccttga gatacgttgc agttataacc cttaatgcta
    18841 gcgttaccgt ccgctatcgt ctatgttcaa gttgtcttaa ttgccagaat ctaacggctt
    18901 tcggcaatta ctccaaaagg gggcgctctc ttttattgat cttacgcatc ctgtatgatg
    18961 caagcagact aaccctatca acgttggtat tatttcccgc agacatgacc cttttagcac
    19021 tcggtatcaa ccataaaacg gcacctgtat cgctgcgaga acgtgtatcg ttttcgccgg
    19081 ataagctcga tcaggcgctt gacagcctgc ttgcgcagcc gatggtgcag ggcggcgtgg
    19141 tgctgtcgac gtgcaaccgc acggaacttt atcttagcgt tgaagagcag gataacctgc
    19201 aagaggcgtt aatccgctgg ctttgcgatt atcacaatct taatgaagaa gatctgcgta
    19261 aaagcctcta ctggcatcag gataacgacg cggttagcca tttaatgcgt gttgccagcg
    19321 gcctggattc actggttctt ggggagccgc agatcctcgg tcaggttaaa aaagcgtttg
    19381 tcgattcgca aaaaggtcat atgaaggcca gcgaactgga acgcatgttc cagaaatctt
    19441 tctctgtagc gaaacgcgtt cgcactgaaa cagatatcgg tgccagtgct gtgtctgtcg
    19501 cttttgcggc ttgtacgctg gcgcggcaga tctttgaatc gctctctacg gtcacagtgt
    19561 tgctggtagg cgcgggcgaa accatcgagc tggtagcgcg tcatctgcgc gaacataaag
    19621 tacagaagat gattatcgcc aaccgcactc gcgaacgtgc ccaaattctg gcagatgaag
    19681 ttggcgcgga agtgattgcc ctgagtgaga tcgacgaacg tctgcgcgaa gccgatatca
    19741 tcatcagttc caccgccagc ccgttaccga ttatcgggaa aggcatggtg gagcgcgcat
    19801 taaaaagtcg tcgtaaccaa ccaatgctgt tggtggatat tgccgttccg cgcgatgttg
    19861 agccggaagt tggcaaactg gcgaatgctt atctttatag tgtggacgat ctgcaaagca
    19921 tcatttcgca caacctggcg cagcgtaaag ccgcagcggt tgaggcggaa actattgtcg
    19981 ctcaggaaac cagcgaattt atggcgtggc tgcgagcaca aagcgccagc gaaaccattc
    20041 gcgagtatcg cagccaggca gagcacgttc gcgatgagtt aaccgccaaa gcgttagcgg
    20101 cccttgagca gggcggcgac gcgcaagcca ttatgcagga tctggcatgg aaactgacta
    20161 accgcttgat ccatgcgcca acgaaatcac ttcaacaggc cgcccgtgac ggggataacg
    20221 aacgcctgaa tattctgcgc gacagcctcg ggctggagta gcagtacatc attttctttt
    20281 tttacagggt gcatttacgc ctatgaagcc ttctatcgtt gccaaactgg aagccctgca
    20341 tgaacgccat gaagaagttc aggcgttgct gggtgacgcg caaactatcg ccgaccagga
    20401 acgttttcgc gcattatcac gcgaatatgc gcagttaagt gatgtttcgc gctgttttac
    20461 cgactggcaa caggttcagg aagatatcga aaccgcacag atgatgctcg acgatcctga
    20521 aatgcgtgag atggcgcagg atgaactgcg cgaagctaaa gaaaaaagcg agcaactgga
    20581 acagcaatta caggttctat tactaccaaa agatcctgat gacgaacgta acgccttcct
    20641 cgaagtccgt gccggaaccg gcggcgacga agcagcgctg tttgcaggcg atctgttccg
    20701 tatgtacagc cgttacgccg aagcccgccg ctggcgggta gaaatcatga gcgccagcga
    20761 gggtgaacat ggtggttata aagagatcat cgccaaaatt agcggtgatg gtgtgtatgg
    20821 tcgtctgaaa tttgaatccg gcggtcatcg cgtgcaacgt gttcctgcta cggaatcgca
    20881 gggtcgtatt catacttctg cttgtaccgt tgcggtaatg ccagaactgc ctgacgcaga
    20941 actgccggac atcaacccag cagatttacg cattgatact ttccgctcgt caggggcggg
    21001 tggtcagcac gttaacacca ccgattcggc aattcgtatt actcacttgc cgaccgggat
    21061 tgttgttgaa tgtcaggacg aacgttcaca acataaaaac aaagcgaagg cgctttcagt
    21121 gctcggtgct cgcatccacg ctgctgaaat ggcaaaacgc caacaggccg aagcgtctac
    21181 ccgtcgtaac ctgctgggga gtggcgatcg cagcgaccgt aaccgtactt acaatttccc
    21241 gcaggggcgc gttaccgatc accgcatcaa cctgacgctc taccgcctgg atgaagtgat
    21301 ggaaggtaag ctggatatgc tgattgaacc gattatccag gaacatcagg ccgaccaact
    21361 ggcggcgttg tccgagcagg aataatggaa tatcaacact ggttacgtga agcaataagc
    21421 caacttcagg cgagcgaaag cccgcggcgt gatgctgaaa tcctgctgga gcatgttacc
    21481 ggcagagggc gtacttttat tctcgccttt ggtgaaacgc agctgactga cgaacaatgt
    21541 cagcaacttg atgcgctact gacacgtcgt cgcgatggtg aacccattgc tcatttaacc
    21601 ggggtgcgag aattctggtc gttgccgtta tttgtttcgc cagcgacctt aattccgcgc
    21661 ccggatacgg agtgtctggt ggagcaggca ctggcgcggt tgcctgaaca accttgccgt
    21721 attctcgatc tcgggacggg taccggggcg attgcgcttg cgctggctag cgagcgcccg
    21781 gactgcgaaa ttatcgctgt agatcgtatg cctgatgctg tctccctggc acaacgtaat
    21841 gcccagcatc tggcgatcaa aaatatccac attctgcaaa gcgactggtt tagcgcgcta
    21901 gccgggcagc agtttgcgat gattgtcagc aatccgccgt atattgacga gcaggaccca
    21961 catcttcaac aaggcgatgt ccgctttgag ccgctcactg cgctggttgc ggcagacagt
    22021 ggaatggcag acatcgtgca tatcatcgaa cagtcgcgta acgcgctggt atccggcggc
    22081 tttctgcttc tggaacatgg ctggcagcag ggcgaagcgg tgcgacaagc atttatcctc
    22141 gcggggtatc atgacgtcga aacctgccgt gactatggtg ataacgagcg cgtaacgctc
    22201 ggccgctatt atcaatgaca agtttttcta cactgcttag tgttcatctt attagtatcg
    22261 cgctttctgt tgggctatta accttacgtt tctggctacg ttatcagaag catccacagg
    22321 catttgctcg ctggacgcgc attgtgccgc cggttgtcga tacggtgtta ttgttaagcg
    22381 gcattgcgtt gatggctaaa gcgcacatcc tgccattttc cgggcaggca caatggctga
    22441 ctgaaaagct gtttggagtt atcatttata tcgttttggg ttttattgca ctcgattatc
    22501 gtcgtatgca cagtcagcag gcgcgcatta ttgccttccc gctggcgttg gtggtgctgt
    22561 acatcatcat taaactcgcc accacaaaag taccgttact ggggtaagtc atgagatcgt
    22621 tagctgattt cgaatttaat aaagcgccat tgtgcgaagg catgatcctg gcttgcgaag
    22681 caatccgccg cgattttccc tcgcaagatg tttacgacga actggagcgt ctcgttagtc
    22741 tggcgaagga agaaatcagc cagcttctgc ctttagaaga gcagctggaa aaactgatcg
    22801 cgctgtttta cggcgactgg ggatttaaag cctcacgcgg cgtttatcgt ctttccgatg
    22861 cattatggct ggaccaggtg ttaaagaatc gacagggcag tgcggtatca ttaggtgcgg
    22921 ttttattatg ggtcgcgaat cgtctcgatt tgccgctgct gccggtgatt ttccctacgc
    22981 agctgatatt gcgcattgaa tgtccggatg gcgaaatttg gctgattaat ccttttaacg
    23041 gtgaatcgtt aagcgaacat atgctggacg gatggttaaa gggaaatatc agcccgtcgg
    23101 cggaactgtt ttatgaagac cttgatgaag ctgataacat tgaggtaatc cgcaaattgc
    23161 tggatacact caaagcctcg ttgatggaag aaaatcagat ggagctggcg ttacgcacca
    23221 gcgaagcttt attacaattc aaccctgaag atccctatga aattcgcgat cgtgggttga
    23281 tttatgcgca actggattgc gaacacgttg cgttgaacga tttaagttat ttcgttgaac
    23341 ggtgtccgga agacccgatc agcgaaatga tccgtgcgca aataaataac atcgcgcata
    23401 aacatattgt gctgcattaa ttaatcgaca ttttactcaa gattaaggcg atcctatgaa
    23461 acaaaaagtg gttagcattg gcgacatcaa cgtagcaaat gacctgccgt tcgtactgtt
    23521 tggcggtatg aacgtgctgg aatctcgcga tctggcgatg cgcatttgcg agcactacgt
    23581 aactgtgacc cagaaactgg gtatccctta cgtgttcaaa gcctcttttg acaaagccaa
    23641 ccgctcctcc atccactctt atcgtggacc gggcctggaa gaagggatga aaatcttcca
    23701 ggagttgaag cagacttttg gcgtgaaaat tatcaccgac gttcacgaac caagtcaggc
    23761 acagcccgtt gctgatgtcg tggatgtgat tcagttgccg gcgtttcttg ctcgccagac
    23821 tgacctggtt gaagccatgg cgaaaaccgg tgcggtaatt aacgtcaaga aaccacagtt
    23881 tgtcagcccg ggacagatgg gtaatatcgt tgataaattc aaagaaggcg gcaacgaaaa
    23941 agtgattctt tgcgatcgtg gtgctaactt cggctatgac aacctggttg tcgatatgct
    24001 gggcttcagc attatgaaga aagtgtctgg taactcgccg gtgattttcg acgtgaccca
    24061 cgcactgcaa tgccgcgatc cgtttggcgc agcttccggt ggtcgtcgtg ctcaggtggc
    24121 tgagctggca cgagccggta tggcggtagg tctggcgggg ctgtttattg aagcgcatcc
    24181 ggatccggaa catgcgaaat gtgatggtcc atccgcgctg ccgctggcta aactggaacc
    24241 gttcctcaag cagatgaaag cgattgatga tctggtgaaa ggtttcgaag aactggatac
    24301 cagcaagtaa tcttttttgc ttgaaaaata aagtcttagc gttctgcgtt aagacttttt
    24361 tcatgggtgc cagatacaaa aaaggccgca ggctgttacc cctgcggccg gtttcgggcg
    24421 catattgcca tcacggcagc ctgacgcccg ttttcacctt acttccggtt acgccaccag
    24481 ctgacaatcg ctgcggcaat aatccccgcc aggatcggtg ctgccaggtc gtgccagaaa
    24541 gtcatggcaa actgcgcgag cgtcatatag ccgccttgtt gtaatgacaa cattttgcgg
    24601 ctattcttga agtgtctggt ttcaagatta gcccccgttt tgttgtcagg ttttacctct
    24661 caacgtgcgg gggttttctc tttccagcaa ccaatgccac cagggataaa gcccccgcaa
    24721 cattgcgcct caccggataa ttccggcttg gtgtggatac tacgtctcaa ttcatcttca
    24781 cttcatccct gaaatgtttg caataaagag tacattccgg cttttcaaca gctgttgcag
    24841 tggtttcgtg caggttgtgg tacaggctcg caattctgat taacgagctg tccaggtagt
    24901 atgaagaaat acaataagca ggaatttatc ttatctctcc cgatttccgt catcggagga
    24961 gatataactg tcaggcaaat atcgtcatca aataggcggc aaacagtgcc agatgcgctg
    25021 cgccattgag cacgttagta cgtccggtgg agaaggagat atggcacagc actaaagagg
    25081 ccaccatcac caccatttct ggcgcaccaa gtgcaaactg caattcgtta cccgtcataa
    25141 aggcaattag cgtgacgaca ggtacggtaa gcgaaattgt tgctaacact gaaccaaaga
    25201 acagattcat cgcgcgctga acctggttgt tcaacactgc ttttaacgca cctaaacctt
    25261 ccggcgacag aatcaacagt gccaccagga agccagtaaa ggcaacaggg gcattcatgc
    25321 tgtcgagcaa tgtctccagc gagctggcgt tcattttggt gactgcaata acggcaatca
    25381 gatggataat caaccagata gcatgccaca ggctgctatg ggcagacgtt ttaccgtggt
    25441 gcgggtcgtc gtcatcactg tcatcttcgt gctcgtagac aaacaaactt tgatgcgttt
    25501 tggcctggat cagcaaaaat acgccataca ttgccgcaga aattaatgct accagtaacg
    25561 cctgaccggt tgaaaaattc gccgcaggca gagccattgg aaataccagt acgattatcg
    25621 ccagggggaa cagcgcaatt aaatactgct tgataccaaa cagattcata tattgggtgg
    25681 caaacttacg accgcccaac aataatgaaa agccaaccag cccaccggta acaatcataa
    25741 tgattgaata gagcgtatca cgcattagcg ttggcgcggc gtcgccggtt gccattaaag
    25801 ctgaaatcaa actgacttca agaataacca ctgaaaggct aagaataagc gaaccgtaag
    25861 gttctcccag gcgatgggct aatacgtccg catgacggac aacactaaag gcgctactta
    25921 aaataccaat aagcgcaaga agattgatgg caatgaccac tggtagtgtc tggctacttc
    25981 cccacaggaa cagcactacc agcaccagaa ccgggaaaat aagcgaagtc tccttgtggc
    26041 gggtttttac cgcctcttga gcatttgaca ttatggttat ccctttgcag atgaatttat
    26101 cgaaaatgta aaaaataggt aggaaaaata acagaaagtg tctggatatc ggtaacattt
    26161 tacgaatttt taccccgctg tcgatttttt actcattggg gcataaaaat aagtattacg
    26221 tttagacaat gtttgtttta gcgtttctta agaacagtct gacatgaaaa ttcttatgtt
    26281 ttggcaagag tagatattgt tgaccacact taatgttcaa ctttgtaaaa ggagtcaacg
    26341 atgccgtata aaacgaaaag cgatctgccg gaaagcgtaa agcacgttct accgtctcat
    26401 gcccaggata tctataaaga agcgttcaac agcgcatggg atcaatataa agataaagaa
    26461 gatcggcgtg atgacgccag tcgcgaagaa accgcgcata aagtggcctg ggctgctgtg
    26521 aagcatgaat atgccaaagg ggatgatgat aaatggcata aaaaatcgta aaaccggtag
    26581 cttagttaaa gctattcgtg cggtgttgcc ttgcaagtgg tccgtggatt gcatattgtt
    26641 ccgttagtgg tttcaaaatg agcggtaaaa atgtccggaa gacaccaaaa agttgtcgca
    26701 gggaagtatg cagtggcgga ggtgtaaggt gataacgcgt gatttcttga tgaatgccga
    26761 ttgtaaaacg gcatttggtg ccattgaaga atcactctta tggtcagcag aacaacgggc
    26821 ggcttcgctg gcggcgacgc tggcttgtcg acctgatgag ggaccggtgt ggatcttcgg
    26881 ttatggatcg ttgatgtgga atccggcact ggagtttacc gaatcatgca ccggtacact
    26941 ggttggatgg catcgcgcgt tttgcctgcg cctgaccgcc gggcgcggga ctgcgcacca
    27001 gccgggacgg atgcttgcac tgaaagaggg cggacgcacc acaggcgtcg cctatcgact
    27061 accagaagag acgctggagc aggaactaac cctgttgtgg aagcgcgaga tgattaccgg
    27121 ctgttatctg ccaacctggt gtcagcttga tcttgatgat ggacgcacag taaacgccat
    27181 tgtgtttatt atggacccgc gacatccaga atatgaatct gacactcgcg ctcaggtcat
    27241 cgcgccgttg attgcggcgg cgagcggtcc gctgggaacc aatgcacaat acctgttttc
    27301 actggaacag gagcttatca aactgggaat gcaggacgat gggctgaatg atttgctggt
    27361 atcggtaaaa aaactactgg cggagaatta tccggatggt gtgttaaggc cgggattcgc
    27421 ctgagtaaac ttccctcata gtggggcgtc agacgccccc ttaaacatta aaatgtgagc
    27481 actttatcgg ctgacagcgt ccattgcgcc agttccacaa gagtaccgat ttccacccca
    27541 tcaatcagag gaagtgtgct aatcccgcgc ccgtcggtac aggttttgca caatttcacc
    27601 ggtacattct gagcggtaag gatctccagc atttgctgaa tgttgtagcc ttcccctggt
    27661 ttttgcccgc gcaacccggc tgtgaccgca tcagacatca ggaacagacg cagatccaga
    27721 ttgctctcct gctctcgtaa cgcaatggcc agccgcaagc tgttaaacaa ggattcgctc
    27781 ccgtaaggtg cgccattggc aacgatcact attttttgca ttatttactc ctgtattcag
    27841 ggaattagac actcatcttc tatcttactg cttctgcagc gtctgaccaa tcggtcacat
    27901 ttttaaggat tttcctgaaa gcgcgagaaa atacgacaaa agttgccagt aatcgttatt
    27961 ctttaaggct atggtttttc attattaccg gaagttaccg acgttttgag ccgtttcgtt
    28021 cctcgcatta tcccgtttta tttactcttg cttgtagcag gcggtacagc taacgcacaa
    28081 tctaccttcg agcaaaaagc ggcaaatccc tttgataata acaatgatgg tctgccggat
    28141 ttaggcatgg ctcccgaaaa tcatgatggg gaaaaacact ttgctgaaat tgtgaaagat
    28201 ttcggcgaaa ccagtatgaa tgataacggg ctggatactg gcgagcaggc aaaagctttc
    28261 gcattgggaa aagtccgcga cgcgcttagt caacaggtta atcagcacgt agagtcctgg
    28321 ctatcaccgt ggggaaatgc cagtgttgac gtcaaagtgg ataacgaagg acatttcacc
    28381 ggcagtcgtg gaagctggtt tgtgccgtta caagataatg atcgttatct cacctggagc
    28441 cagcttggtc ttactcagca ggataatggg ttggtgagca atgtgggcgt tgggcaacgc
    28501 tgggcgcgcg gcaactggct ggtgggttat aacacttttt atgacaactt gcaggacgaa
    28561 aatcttcagc gagcgggctt tggtgccgaa gcgtggggcg aatatttgcg attatcggca
    28621 aacttttatc agccgtttgc tgcatggcat gaacagacag ccacgcagga acaacggatg
    28681 gcgcgcgggt acgacctgac agctcggatg cgcatgccgt tctatcaaca cctcaatacc
    28741 agtgtcagcc tagaacagta ttttggtgat cgtgttgatt tgtttaactc tggtacgggt
    28801 tatcacaatc ccgtcgcgtt gagtctggga ttaaattaca cccctgtgcc attagtcact
    28861 gtgacggccc agcataaaca gggggaaagt ggcgaaaatc aaaataacct cgggctgaat
    28921 cttaattacc gctttggtgt accgctcaaa aaacaacttt ctgcgggcga ggttgccgaa
    28981 agtcagtcgt tacgtggtag tcgctatgac aatccgcagc gaaataatct accgactctt
    29041 gagtaccgac agcgaaaaac gttaacggtg tttctggcga caccgccgtg ggatctaaaa
    29101 cctggcgaaa cagtgccgct gaaattacaa atccgcagtc gttacggtat tcggcaactg
    29161 atttggcagg gcgatacgca gatattaagt ttgacgccgg gcgcacaagc caacagcgcg
    29221 gagggctgga cgctgatcat gcctgactgg cagaacgggg aaggggcaag caatcactgg
    29281 cgattgtctg tggtggtgga agataaccag gggcagcgtg tctcctccaa tgagatcacg
    29341 ctaacgcttg tcgaaccgtt cgatgcattg tcaaacgacg aactgcgctg ggaaccgtaa
    29401 tcagaaaatg cgctcctgat gcacccatac cgctgcttcc acgcgagact tgagcttcat
    29461 tttcttcagc atgtgcttga cgtgcacttt tactgtgctt tcggtgatat ccaggcggcg
    29521 ggcaatcatc ttgttcggca aaccctgggc aatcagcttg agaatatcgc gctcgcgtgg
    29581 ggttaactgg ttaacatcgc gctcagtagt ggcacggtta gcgcgcaagc tggcggccag
    29641 aacaggcgtt aatgcttcgc ttaataccat ttcgccagca gctgcctgat gcaatgcttt
    29701 cagcagatct tccggttcca tatcttttaa cagatagcca tccgcgccgc gtttcagtgc
    29761 ggtgaccaca tcttcttcat ggttagagac gctgaatacc acaatgcgcc ctgagaggga
    29821 cttttcgcgc agtttatcca gcgtttccag accgttcatg ccgggcatat tgagatctaa
    29881 caggatcaga tcgggatcaa gagattccgc cagttcaata ccctgttcgc cattactcgc
    29941 ttcgccaacc acggtgatat ctggtgccat actgataagc tgttttacgc cagttcgcag
    30001 catcgggtga tcgtcaatca gcaggatagt agccggttcc tgattactca tgggtatctc
    30061 cttggacgtc tgtgaaagtt ttttcgggaa taaaggtgac caccacttcg gtgccacctg
    30121 attcacgacg gcggacgcgg caatcgcctc gtaaactttg cgcacggtcg cgcattatta
    30181 tcatgccgta gtgattgctg cggatggcat tttcaggcac gccacagccg ttatcctgga
    30241 cggtcagttt gacctgatta tcgttttgcg ccaccgtcac cacgacctca ctcgcttgcg
    30301 aatgtttgag ggcgttactt aatgcctcac gggcaatttg caacaagtgg attgcctgat
    30361 gtgaaggcac cagacgaggc ggcaattgat aatccagctt caccgggaag ccaaatttgg
    30421 cgctgtactc ttcgcaactc gcttccagcg ccggacgtaa tccaggctcg gtgagctgca
    30481 agcggaatgt ggtgagcaat tcacgcaact gcgcccagga tgcattcagt tcgttacgga
    30541 tctgacttaa cagttcgcgg ctgctttctg gcagcgcatc gccctgcatc tgtaaacaac
    30601 tcacctgcat cttcatgcaa gagagagatt gggcaataga atcatgcagt tcgcgcgcaa
    30661 tggtggcacg ctcttccatc acgatcaact gttgctgacg ttcctgatgg cgatccagcg
    30721 ccagcgtggc ggtaagttgt tcaaccaggg tatccaccag ttgttgttgg tcatggctaa
    30781 gatgacgccc ctgcggcagg gtcgccagca aaataccgta ctgcgtatga gagtcagcca
    30841 gccgccactt cagggtcgtg ccgcgatcac caacgggtaa tacgccgcgc gggcagagct
    30901 ggcagccttt atcatcacaa gtcatatctg gctggcaggt aaactcctga tgattctctt
    30961 catcatcagt gtcatacacc cgcagttcga tatcacgtag cagggttaaa ttctgtaagc
    31021 cgttgagtac cggtgacagg cgttcacaca gcggggcgcg ggaatgcaaa cggcggttag
    31081 cctgccataa aaaagagagg atctgatttt tatgctccag cccggcggtt ttctcctgaa
    31141 cccgctgctc aagtacggca taactttcgg ccagttctgc agacatattg ttcaacgcag
    31201 ttccaagcat cgccatttcg ttgcgcccgc tgatgtttgc gcgttgggta aaatcgcgat
    31261 ggctgacggc actcgccatt gccagcagtt gccgccacgg ttgtagcagt cgcgcccgca
    31321 accagataat agtgaacacc agtaaaagtg ccataaatac cgccattacc cgatggacca
    31381 gtaccactgt ctcaatacgc atttccgtgg tgcggtcaaa accagatacc agctgatcaa
    31441 gcccggcaac aaactggctg acatccgctg acaccgtttc tcggttttgt gcacgcatca
    31501 gcgcagggat cagttcatta cgccagtaat cttgtaagcc ctgtaattgc gccagttgtc
    31561 cgtctcgttc tgctgctcga gtcaactcgg cgctaaatgc cgtttgttcc atctctttaa
    31621 ttaagggctt gtctttctcg cttaatggca ctgccgccaa cagacggtaa ctttgcatgc
    31681 gcagcgatcc cgctttgttg atcgcatggg cgctgccctg aacgccttgc accagccagc
    31741 cagaaaccgc catccctgcc agtccaatag cagtagaaag caacacaata agcgcaacct
    31801 gattaaccag ggtgagcgga gagagacaac gtttaagcat gtaaacccct tccttcaggc
    31861 tttaaatgag caataacctt aatgaatgtg acgatatatt ctggaatggc agtattctcg
    31921 gctattggct gaagtatacc catagccgga aagagttact ccttatttgc cgtgtggtta
    31981 gtcgctttac atcggtaagg gtagggattc tacagcaccg tgaaaaatct cataattttt
    32041 atgaaatcac tgtactcact atgggtaatg ataaatatca atgatagata aagttatctt
    32101 atcgtttgat ttacatcaaa ttgcctttag ctccagacac taaggtggca gacatcgaaa
    32161 cgagtatcag aggtgtctat gagtcactca tctgcctccg aaagggctac tggagctgtc
    32221 ataacagatt ggcgaccgga agatcctgcg ttctggcaac aacgcggtca acgtattgcc
    32281 agccgcaacc tgtggatttc cgttccctgt ctgctgctgg cgttttgcgt atggatgttg
    32341 ttcagcgctg ttgcggtgaa cttaccgaaa gtcggtttta attttacgac cgatcagcta
    32401 tttatgttga ctgcgctgcc ttcggtttct ggcgcgttat tacgtgttcc atactccttt
    32461 atggttccta tcttcggtgg tcgtcgctgg acggcgttca gcaccggtat tctgattatt
    32521 ccttgcgtct ggctgggttt tgccgtgcag gatacctcca cgccttatag cgtcttcatc
    32581 atcatctctc tgctatgcgg ctttgctggc gcgaacttcg catccagtat ggcaaacatc
    32641 agcttcttct ttccgaaaca gaagcagggt ggcgcgctgg gtctgaatgg tggtctggga
    32701 aacatgggcg tcagcgtcat gcagttggtt gctccgctgg tggtatcact gtcgattttc
    32761 gcagtatttg gtagccaggg cgtcaaacag ccggatggga ctgagctgta tctggcgaat
    32821 gcgtcctgga tatgggtgcc gttccttgcc atcttcacca ttgcggcgtg gtttggcatg
    32881 aacgatcttg ctacctcgaa agcctccatc aaggagcagt tgccggtact caaacggggt
    32941 catctgtgga ttatgagcct gctgtatctg gcaaccttcg gctccttcat cggcttctcc
    33001 gcgggctttg cgatgctgtc aaaaacgcag ttcccggatg ttcagattct gcaatacgct
    33061 ttcttcgggc cgtttattgg tgcgctggcg cgttctgcag gtggtgcatt atctgaccgt
    33121 ctgggcggaa ctcgtgtcac gctggtgaac tttattctga tggcgatttt cagcggcctg
    33181 ctgttcctga ccttaccgac tgacgggcag ggcggaagct tcatggcgtt cttcgcggtc
    33241 ttcctggcgc tgttcctgac agctgggctg ggtagtggtt ccactttcca gatgatttca
    33301 gtgatcttcc gtaaactgac aatggatcgc gtgaaagcag aagggggttc tgacgaacgt
    33361 gcgatgcgtg aagcggcaac cggcacggca gcggcgctgg gtttcatctc tgcgattggc
    33421 gcgattggtg gcttctttat cccgaaagcg tttggtagct cgctggcatt aacgggttcg
    33481 ccagtaggtg caatgaaagt atttttgatt ttctatatcg cctgcgtagt g
//
LOCUS       DAZXUK010000017        33102 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZXUK010000017 DAZXUK010000000
VERSION     DAZXUK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33102
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.017"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(56..418)
                     /locus_tag="ACH3L8_000673"
     CDS             complement(56..418)
                     /locus_tag="ACH3L8_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFT4083917.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(435..1340)
                     /gene="yhfZ"
                     /locus_tag="ACH3L8_000674"
     CDS             complement(435..1340)
                     /gene="yhfZ"
                     /locus_tag="ACH3L8_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFT4083918.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(1610..2614)
                     /gene="trpS"
                     /locus_tag="ACH3L8_000675"
     CDS             complement(1610..2614)
                     /gene="trpS"
                     /locus_tag="ACH3L8_000675"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFT4083919.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(2607..3365)
                     /gene="gph"
                     /locus_tag="ACH3L8_000676"
     CDS             complement(2607..3365)
                     /gene="gph"
                     /locus_tag="ACH3L8_000676"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFT4083920.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(3358..4035)
                     /gene="rpe"
                     /locus_tag="ACH3L8_000677"
     CDS             complement(3358..4035)
                     /gene="rpe"
                     /locus_tag="ACH3L8_000677"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFT4083921.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(4053..4889)
                     /gene="dam"
                     /locus_tag="ACH3L8_000678"
     CDS             complement(4053..4889)
                     /gene="dam"
                     /locus_tag="ACH3L8_000678"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFT4083922.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(4996..6288)
                     /gene="damX"
                     /locus_tag="ACH3L8_000679"
     CDS             complement(4996..6288)
                     /gene="damX"
                     /locus_tag="ACH3L8_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFT4083923.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(6380..7468)
                     /gene="aroB"
                     /locus_tag="ACH3L8_000680"
     CDS             complement(6380..7468)
                     /gene="aroB"
                     /locus_tag="ACH3L8_000680"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFT4083924.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(7525..8046)
                     /gene="aroK"
                     /locus_tag="ACH3L8_000681"
     CDS             complement(7525..8046)
                     /gene="aroK"
                     /locus_tag="ACH3L8_000681"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFT4083925.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(8447..9685)
                     /gene="hofQ"
                     /locus_tag="ACH3L8_000682"
     CDS             complement(8447..9685)
                     /gene="hofQ"
                     /locus_tag="ACH3L8_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFT4083926.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(9597..10003)
                     /gene="hofP"
                     /locus_tag="ACH3L8_000683"
                     /pseudo
     CDS             complement(9597..10003)
                     /gene="hofP"
                     /locus_tag="ACH3L8_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(9993..10433)
                     /gene="hofO"
                     /locus_tag="ACH3L8_000684"
     CDS             complement(9993..10433)
                     /gene="hofO"
                     /locus_tag="ACH3L8_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFT4083927.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(10417..10956)
                     /gene="hofN"
                     /locus_tag="ACH3L8_000685"
     CDS             complement(10417..10956)
                     /gene="hofN"
                     /locus_tag="ACH3L8_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFT4083928.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(10956..11735)
                     /gene="hofM"
                     /locus_tag="ACH3L8_000686"
     CDS             complement(10956..11735)
                     /gene="hofM"
                     /locus_tag="ACH3L8_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFT4083929.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            11855..14407
                     /gene="mrcA"
                     /locus_tag="ACH3L8_000687"
     CDS             11855..14407
                     /gene="mrcA"
                     /locus_tag="ACH3L8_000687"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFT4083930.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(14572..15132)
                     /gene="nudE"
                     /locus_tag="ACH3L8_000688"
     CDS             complement(14572..15132)
                     /gene="nudE"
                     /locus_tag="ACH3L8_000688"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFT4083931.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            15452..17587
                     /gene="igaA"
                     /locus_tag="ACH3L8_000689"
     CDS             15452..17587
                     /gene="igaA"
                     /locus_tag="ACH3L8_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFT4083932.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            17652..18320
                     /gene="yrfG"
                     /locus_tag="ACH3L8_000690"
     CDS             17652..18320
                     /gene="yrfG"
                     /locus_tag="ACH3L8_000690"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFT4083933.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            18331..18732
                     /gene="hslR"
                     /locus_tag="ACH3L8_000691"
     CDS             18331..18732
                     /gene="hslR"
                     /locus_tag="ACH3L8_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFT4083934.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            18757..19626
                     /gene="hslO"
                     /locus_tag="ACH3L8_000692"
     CDS             18757..19626
                     /gene="hslO"
                     /locus_tag="ACH3L8_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFT4083935.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(19770..21521)
                     /locus_tag="ACH3L8_000693"
     CDS             complement(19770..21521)
                     /locus_tag="ACH3L8_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFT4083936.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            21900..23522
                     /gene="pckA"
                     /locus_tag="ACH3L8_000694"
     CDS             21900..23522
                     /gene="pckA"
                     /locus_tag="ACH3L8_000694"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFT4083937.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(23598..24950)
                     /gene="envZ"
                     /locus_tag="ACH3L8_000695"
     CDS             complement(23598..24950)
                     /gene="envZ"
                     /locus_tag="ACH3L8_000695"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFT4083938.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(24947..25666)
                     /gene="ompR"
                     /locus_tag="ACH3L8_000696"
     CDS             complement(24947..25666)
                     /gene="ompR"
                     /locus_tag="ACH3L8_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFT4083939.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            25894..26370
                     /gene="greB"
                     /locus_tag="ACH3L8_000697"
     CDS             25894..26370
                     /gene="greB"
                     /locus_tag="ACH3L8_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFT4083940.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            26467..28788
                     /locus_tag="ACH3L8_000698"
     CDS             26467..28788
                     /locus_tag="ACH3L8_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFT4083941.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            29226..29453
                     /gene="feoA"
                     /locus_tag="ACH3L8_000699"
     CDS             29226..29453
                     /gene="feoA"
                     /locus_tag="ACH3L8_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFT4083942.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            29470..31791
                     /gene="feoB"
                     /locus_tag="ACH3L8_000700"
     CDS             29470..31791
                     /gene="feoB"
                     /locus_tag="ACH3L8_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFT4083943.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            31791..32027
                     /gene="feoC"
                     /locus_tag="ACH3L8_000701"
     CDS             31791..32027
                     /gene="feoC"
                     /locus_tag="ACH3L8_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFT4083944.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            32230..>33102
                     /gene="rpnA"
                     /locus_tag="ACH3L8_000702"
     CDS             32230..>33102
                     /gene="rpnA"
                     /locus_tag="ACH3L8_000702"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFT4083945.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAAS"
BASE COUNT         7966 a         8961 c         8393 g         7782 t
ORIGIN      
        1 taaatattca tttttttgaa tatttaggtt gtgcgacgtg gatttgtcgg gagcttcaaa
       61 cctcttcgtt agcagccatc cataatccat aaagattggc cagaaaatag ccttcttcac
      121 acgggttaac ttccagagcg aattccttca acaactcctg atgcaattgc acaacggctt
      181 gccagtggct ggattgcgcc agttccgcca gcaactcgtt atccagcggc tctatttctt
      241 caccacggcg actgcgcatc agtgcactcg ccatatgtgt catcgccatc gttccttgct
      301 cactgcgcac cggcagatgg cactcttttt ccagtacgtt gacgacctgc atcatgcctt
      361 tgcagacgtc cttatcaata acgcctgcct cgcaaagcag gttgagtctg gtttccatat
      421 cagatgcctt tcgtttaata actgggttcc tgttccccac tcaccacccg ttgttgatgg
      481 gcaagcaggg cgtgtttatc tacaacagca cgcagcaatt gttgcattgg gtaatcatcg
      541 actcgcgtca ggaccacggc ttcggtggcc tgtaaaaatc gcggatcgtc tgttagcggc
      601 gtcgcctcta atcccagcat ggtcagttcg ttttccgcca ccacgttcca gatcaccgca
      661 tcgacatcgc ctttaacaat gcgttgtaaa ctctcgtgat aagagagatc gactcgttcc
      721 acatcactat cgccaaaaaa gacatcggtc atgattttct gatccgccga acgactatcg
      781 agacctactc gcttaacatt tccagactca cctttgcggc atatcagttg atgttcacca
      841 acatacgtat gcgggccaag ctcgagggcg atgcacaaat tgttttggct gagataactt
      901 tccgccgcca ggcgggaaac tactgccatg tcatagacac cgttaagtaa acactccacg
      961 cgaatatccg cgccgcgcat atgcgcatag taaaaaggaa tgccatcaaa ctgggctttc
     1021 aatccgctcg ctaaaccttc gtacaagcgg gtatagggca aaggcatcgc acataccacg
     1081 ttgttgatat ccacatgact cagcaatgct ttgttatcca tctcgacaag ataactgcca
     1141 ttgcgcccac ggcgttcaat ccgtatcgct ccgcttgatt ccagcgtttt caacgcggcc
     1201 tgcgtcaggc caaccgatga acggcattca gttgccagct catctatggt tttcagtcga
     1261 ttaccgcatt tttcacccaa caaataacgg gccagtgtcg tgatgacgac gccttctttt
     1321 ttgataaacg ttcgacgcat aatatatttt caataaaatg aatatttata tcttcattaa
     1381 aatgaagata agtggcgacg acgagatgtg gaaacggcga ggcgcttcac attttttcgg
     1441 gttgttgcga aaagaggata aaaaaaccgg gtttccccgg tttcagagtg atgacgaagt
     1501 gatttattcg acaatgcgag caaagggtaa ttcccactgg cgcttgcctg ctcccctcac
     1561 cctaaccctc tccccaaagg ggcgagggga ccgtccgagc gttttgatat tacggcttcg
     1621 ccacaaaacc aatcgcttcg tacaccgctt tcagcgtacg ggaagcgtgt acactggctt
     1681 tttctgcgcc gtctttcatc acctgttgca ggaaggcttc atcgttgcgg aaacggtgat
     1741 agcgttcctg caattcagtc agcataccgg aaacggcatc agccacttca cctttcagat
     1801 gaccatacat cttgccttcg aactgttttt ccagttctgg gatgctctgg cccgttaccg
     1861 cagaaaggat atccagcagg ttggaaacgc ccgctttgtt ctgcacatcg tagcgaacta
     1921 ccggcggctc gtcggagtca gtgaccgcgc gtttgatttt cttcactacc gatttcggat
     1981 cttccagcag gccgataacg ttattgcggt tatcgtcaga cttggacatc ttcttggtcg
     2041 gctccagcag cgacattacg cgcgcgccag atttcggaat aaacggctcc ggcaccttaa
     2101 agatctcgcc atacagcgcg ttgaaacgct gggcgatatc gcgactcagt tccaggtgct
     2161 gtttctggtc ttcacccacc ggtaccagat tagtttgata cagcaggatg tccgcagcca
     2221 tcagcaccgg atagccaaac agaccagcgt tgatgttctc ggcataacgc gcagatttat
     2281 ctttaaactg cgtcatgcga ctcagttcgc cgaagtaggt atagcagttc agtgcccagc
     2341 ctaactgcgc atgttctggt acgtgggact gaacaaaaat ggtgcttttc tcaggatcga
     2401 taccacaagc cagatacaag gccagcgtat ccaacgtcgc tttacgcagc ttctgtgcat
     2461 cctggcgcac ggtgatcgcg tgttggtcaa cgatacagta aatgcaatgg tagtcatcct
     2521 gcatgtttac ccactgacgc agcgcaccca tgtagttacc aatggtcaat tcacctgagg
     2581 gctgtgcgcc actaaaaacg atgggcttag tcatttttcg attcctgatt ttcgctatgc
     2641 ggaagcccta atgcgggcag aaggtcattt atagactgat aaattacatc aggctggctg
     2701 agatcgatag cctcgccgta gttgtatccg taggttaagc caactgatgg gcaacctgcc
     2761 gcttttgccg cctgaatatc attccgtgag tcgccgacaa acagcatctg ttgtggggca
     2821 attcccatcc gctcagccac cagtaacagc gggtccggat gcggtttttt gttttgcaca
     2881 tcatcgccgc cgataaccac gctaaagtat ttggcaatat ctaaggcttc gagcagcggc
     2941 gcgacgaacg gcgtcggttt gttggtgacc aagcctagcg gcaggccttt agcctgcaac
     3001 gcgcccaacg tatcggcaac gtgcgggaac aaaaacgtcc cctcttcggc aacctcgcca
     3061 tagtagcgat cgaacagttt acgcagaata cgtacctgtt cttctgccgg aatgtcgtca
     3121 tcaacgggcg gtttccccat tgttttacgc agagtcgcac gttcctgacg cgcccaggtc
     3181 aatgcgcgct ccatcagaac atctgcgccg ttaccaatcc aggtaataac gcgttcttca
     3241 cctgcgacgg gcaactccag cgcatacagc gccatatcta ccgcagcagc aagaccagga
     3301 gcactgtcga ccagcgtacc atcaagatca aaagcgacgc cgcgaatatc ttcaaactta
     3361 ttcatgactt acctttgcca gttcactgcg catttcatca atgacttttt tatagtctgg
     3421 ctggtcgaag attgccgaac cggcgacgaa catatccgcg cccgccgcag cgatttcgcc
     3481 aatgttgttc accttcacgc caccgtccac ttccagacga atgtcaaagc cagactcgtc
     3541 gatacggcga cgtacttcgc gcagtttatc cagtgtttga ggaatgaaag actgaccgcc
     3601 gaaaccaggg ttgacggaca tcagcaggat cacatccagc ttatccatca cgtaatccag
     3661 atagctcaga ggtgtcgccg ggttaaatac cagacccgct ttacagccat tttctttaat
     3721 cagttgcagc gtgcggtcaa catgctcgga ggcttctgga tgaaaggtaa tgatgctggc
     3781 accagcggca gcgaaatcag gcacaatgcg atcgacgggt ttcaccatca gatgtacgtc
     3841 gataggggcg gtaatgccgt agttacgcaa ggatttcagc accattggcc caatcgtcag
     3901 attgggaaca tagtggttat ccatgacgtc aaaatgcacg acatcagcgc cagctgccag
     3961 ggcttttgcg gtatcttcac ccaggcgggc aaaatcagcc gacagaattg agggggcaat
     4021 caaatactgt ttcatccgct tctccttgag aattattttt tcgcgggtga aacgactcct
     4081 ggtttgtaca aagccagcag ttcgtccacc tttttacgtg tgccgccgtt gctgcttata
     4141 ctgcgtcgaa ctttgacgac atgcaatttt gcgcgctgat accactcacg cgttaacatc
     4201 gtatcgtgat tggagatcag cactggaata tggcgctcaa ccagaccttc ggcgatctcc
     4261 gccagatgcg cttgttgttc aagcgtaaaa ctgtttgtgt gatacgccgt aaagttggcg
     4321 gtcgcagaca gcggtgcata aggcggatcg caatagacga cggatgcatc atctgcgcgc
     4381 tccatgctat cggcgtaaga ctcacaatag aaaaaggcat tctgcgcttt ttcagcgaag
     4441 tgatacaact ctgcttccgg gaaatagggt tttttgtagc ggccaaacgg cacgttaaac
     4501 tcaccgcgca gattgtaacg acacaggccg ttgtaaccgt agcggttcaa atataaaaac
     4561 agtaccgccc gacggaacgg atcctggctt ttgttgaact cttcgcggaa ctgatagtaa
     4621 acctcggcgc aatttgtttc gggaacaaac agctcgcgtg cggcctgtac gtactcatca
     4681 gtacgcatct tcacaatgtt atagagactg atcaggtcgc tattgatatc ggcaaggata
     4741 taacgagaaa agtcggtgtt gagaaacacc gacccggcac ctacaaaagg ctcaaccaga
     4801 cattcgccct tgggcaaatg ccgtttaata tcatcaagca ggggatactt gccccctgcc
     4861 cacttcaaaa aagcgcgatt tttcttcatg ccgattaact aattacacct tctccggctg
     4921 tggagaaagc tccgacagca tccagcgctt caggcagctc ccgcaacgat aaaccattgc
     4981 gggagataac cttgattact tcagatcggc ctgtacctga cgcagcggtt tcgcccacgg
     5041 gtttttggcc tgaacatctg ctggcaatgt agataccgct tttttcgcct cttctttcga
     5101 agcgtacacg ccagaaacca ggacatacca cggctgacca ttacgcgtcg tttcatagac
     5161 aacgtagttt ttcagattct ctttcttcgc ccaaccgttc aggttgtcgt agttagagga
     5221 actgctcagc tgcagagtgt aatggctgga cggtgccgat ttcaacgaac caacattacc
     5281 tgcggtcttc cctccagcgg ctggtgttgc cgtggtttgc gccggggatg ccgtctgtac
     5341 tggagccgta gtcgccgtct cttttggtgc tggcgtagaa gtcgcagccg gtgccttcgt
     5401 gctcgccaca ggggcagctg gttcaggttc agtacgcttc ggcgtctgtg ctaccggctt
     5461 cggctccgtt ttcacggttg cttgcggttt tttcggttca atcaccgcct gctgacgaac
     5521 tgggcgcgta gtggacggac gttcagcggt ttgcgttttc gccgtgtcac gcgatgcatt
     5581 gccattgcga acaggcgcaa ccgttgcggg ttcagtcggc aacgtggaat tgaccgccac
     5641 attgttcagc tgttgctgat tttgtggctg ggtcagcgca ttgttcaggt caccctgcac
     5701 ttcaacacgt tgttgaccat ccgttgccac cggggtttgc ccttgagtcg gcgtagaaga
     5761 gatcggtggc agagaaacat cctgctgagt attttccgca gacgtggttc ccggcgctgg
     5821 ctgcacgcca ttcgcctgat cggtcgcatt gccagcaaga tcaatactct tctcgccaga
     5881 cgcggtttga tcgctggaag tggtcgaggg ggcttttagc gcagaaccga taccgatgat
     5941 caacagcagt agaaccagaa tgccgacgcc catcatcata tactgacgag aagcgggttt
     6001 actggctgct tttttgcgct tacgcggacg acgctctacg cgctcttcat ccacggtttc
     6061 atcttcggat tcgtcaattt cttcttcgat ttccggttcc tcattgcgct ctttttgcgc
     6121 acgagtcgga cggcgatcgt cagtgtcatc aagttcaata tcatcaaaat tgatctgcgg
     6181 ttcgccacgt tcagtacgct cagaacgttc agaagattga cgagaacgac cagtacgacg
     6241 atcgctggga tcgggtttca gctcgtcttc tggtttgaat tcatccattt aacaccccac
     6301 taaaaggtta atgcttacca cgttgcaatt aacctgaagc taatagaccg cttgataagc
     6361 ggcctgacct ttcttgttgt tacgctgatt gacaatcggc aatggcgtta agaacaagct
     6421 cgtgcgaaac gccgctgcga acttcactct taccaattgc caacggaaga attaagcgca
     6481 tctctcccgc aaggactttc ttgtcacgca gcatatgcgg taaatacgcc tgcgcggaca
     6541 tttcgcgcgg cccattgacc ggtaacccag cccgcgtgag cagggttata atacgctgcg
     6601 tttcggcaga actaaactgc ccgagacgtt ccgacgtccg cgccgccatc accatacccg
     6661 cagcgaccgc ttcaccatgt aaccaattgc cataccccat ttcagcttca atggcatgac
     6721 caaaggtgtg tcccagattc agtaaagcac gtaacccggt ttcgcgctcg tcggcggcga
     6781 caacttctgc cttcagttca caacaacggc gaatacagta cgccattgcc ggaccgtcca
     6841 gacgcaacaa cgcatccaga ttctcttcca gccagttaaa aaacgcaccg tcaagaataa
     6901 tgccgtattt gatgacttct gccagccccg acgctaactc acgcggggga agcgttttca
     6961 gacagtcgag atccaccacc actgaagcag gctggtagaa cgcgccaatc atgtttttac
     7021 cgaggggatg gttgaccgca gttttgccgc caacggagga atcgacctgc gacagtaacg
     7081 tcgtcgggac ttgaatgaaa cgaacaccgc gctgataact cgccgccgcg aagccggtca
     7141 gatcgcccac tacgccgccg ccaagcgcca ccagcgtagt atcgcgacca tgcggctttt
     7201 gtaacaacgc cgtaaagacg gtatcgagta cagctaggct tttatactgc tcgccgtcag
     7261 ggaggataac gctatcgacg ttaacacccg cctgttcaag tacgccgcgg accttatcga
     7321 gatacagagg agccagggtt tcgctggtga ccaacatgac ctgctcgccc gatttcagcg
     7381 gtaagaatga agctggttca ttaaacaaac cagatgcgat ggtaattggg taactacgtt
     7441 ccccgagagt aacgacaatc ctctccataa cgcgacatcc accttaatta ctgtacccgc
     7501 agacgagtgt atataaagcc agaattagtt gctttccagc atgtgaataa tctggtttgc
     7561 aaccacttta gcgctttgat catcagtacg aatggtcacg tcggcaatct cttcatacag
     7621 cggattgcgt tcattggcca acgcttccag aacttcacgc ggcggtgttt caacgtgcag
     7681 caacgggcgt tttttatcac gctgcgtgcg tgcaagttgc ttttcgatgg tcgtttcaag
     7741 ataaacgaca acgccacgag cggaaagacg gttacgcgtt tcacgggatt tcacagagcc
     7801 gccgccagta gccagcacaa taccctgttt ctcggtcaac tcattgatga ccttttcttc
     7861 gcgatcgcgg aagccttctt cgccttctaa atcgaaaacc cagcccacat cagctccggt
     7921 tcgtttctca atctcttgat cggaatcgta aaattccata ttgagttgtt gagctaactg
     7981 gcgcccaata gtgctttttc cggcacccat aggcccaacc agaaagatat tgcgtttctc
     8041 tgccattttt tcggtactac taagactatt cgttaatgat aaacccgctt cgctcagaga
     8101 gcgccgcagg acatgaactg aaacctcata agatattgcg agagtcagac tgaaaattat
     8161 ctcaatactc cagcgggttt ggcaactgaa taaatcaccc atccctctgc atatctggtc
     8221 gctgcaagcg cgctgccttg ctaccaccgc tctggcgata aatcaccggg taagattagc
     8281 gtaaaaaaga cagcaaaatg ccgcctgaat gataaatcat catcatggaa cgcaggcagc
     8341 gaagagcacc aactctcaaa tcggtactcc ttgtatgcta aatacctgcg cgcgtcaaat
     8401 agatgaaaca cgttcagcat aaaaacatta ccgcttttac ggctgtttac tcactggaaa
     8461 ccagtcgtgg cgtgataaac accactaact cgcgtcgttc atcttctttt ccgtcatgac
     8521 gaaataattg cccgaaccag ggaatgtcgc caagcaacgg tacgctatcc tgacccgatt
     8581 tatttttacg ggtaaaaatg ccgcccagcg ccaacgtttc tccgcttttt acctcgacct
     8641 gcgtttcgat ctcctgctta tcaatcgcca gcacttcgcc atcggcctgc tgtagcacct
     8701 gccccggaac gttctggctg atgtgtaatt tcagccggat gcgacctttt tgtaacaccg
     8761 tgggcgtgac ctccatcccc aggacggcct ctttaaattc caccgacgtc gcgccacttt
     8821 ccccgctgga aacctgatat ggaatttcgc tctcctgttt aatgctggca ggctgaagat
     8881 gtgaggccag cagacgcgga ctggcgataa tatccagctg ctgtttttgt tcgagcgcgg
     8941 aaagctcaag atccagcaag cgtccgttga tgcgcccaat gttaaaaccg acatgcgttg
     9001 tcgccgtcgc tacggagagg tcgctaccaa gcgtggtgac ttgcccaacg ccaccagcgt
     9061 gttgcgcatc ggccagcgtc catttcacgc ctaactcacg caaacttttt tcattaatgg
     9121 tgacaatatg cgccgacagc tcaacctgcc cgaccggcag atccatttgc gctacccact
     9181 gttcaagcgc gcttaacgcc gttttgttat ctcgtagcaa aaggcgattg gtgcgtttat
     9241 cgacggtcat actccctttg gcactcagta gcttctcccc cgctttcgcc agttctcccg
     9301 cgtcggcgta ttgcagggtt atactgcgat tttccagcgg cagatttgcc tgcgcccgcg
     9361 cctgctccgc ctcctggcgg gcgatattgt cattctgcca ggcaatggaa tgcactgaga
     9421 gaatgttgcc ttcctgccgc gttatcagtc cggcgctttt cactacagtt tgtagtgcct
     9481 gcttccaggg aacatctgtt agatgtaacg acaccgtacc gctgacgtct ggcgacacga
     9541 ccaggttcaa cttctcctgt tcagccagcg cctgcaacac ctgagctacc ggaacgtcat
     9601 ccaccatcag cgtcactttt tgcggctttg ccgcctgtac gccgggtatc agcatcaaca
     9661 gtagtgcggc tatccattgc ttcatttgta tctccttgcc gttgccacaa ccattgtggc
     9721 ggttcgcagt ttgtcccggt acccagcgtt agtacgtctg gcgtcagctg taaaattgtc
     9781 cagccgtttt ccagcacatc gttttgctgc acccgtcgcc atttcttttg cccgtctttt
     9841 attacaccga tgatgcgctc gcctcgccct accatcccct gatagcgcca ctggctaagt
     9901 tcgctaatcc ggcatataga tcttccggcg atttaaaagg gtcacgcata ccggttaaaa
     9961 ggcacaatgc aatacctgcc aacaaccagc gtttaaccct catgcggcat ctccagttgt
    10021 agcgtgaaca aaagatcatc accttccacg cttaacgaaa aacggctcac gctgacgttg
    10081 cgctctgcca gccgtgtaaa tgccgacggc actgcttccc acagcgtttt caacgccaac
    10141 tcacctccct gcgcggatgg atgccaggaa accagttgcg cgccggataa ctgaaaatcc
    10201 agtggcgaaa agggcagcgt tttttcctcg ctgaagggag tggtgtctac caggcgatac
    10261 agtgcggccc actgctggtg atgacttgcc cgtagtcgaa ttaatgcgtc gcgctcttca
    10321 tggtgtgtcg atgatagaaa aatgagcgta actaacatca gcaaccagaa tgcccagcaa
    10381 aactggcgga ggcggggtga tgtggcgaac caccagtcaa agaacatgtt catcgctaac
    10441 cttccttgtt aactgatact caaattgcca gcgtccctgc gcatcctgct gcgtggctcc
    10501 ccgctgattg agatgaaaag acgcatcctg gcggagtgac gtttctagtg cgtttaacgc
    10561 ggtaatgctt gttgtcagcc ccttgatctc cagcgttccc tgctgccagc ttatcgttgt
    10621 cagccaggcg tgctcgggta aaagcgccgc cagtgcttcc agcgcagatt gccagtcgcg
    10681 ggtaaattgt cgctggcgct gccgttgcga acgctgttcg cgtaactgct gttgctccag
    10741 caaacgtggc ttcgttatct gtaagctgcg ggcgagttgt tgttccgcct gaagcaaaac
    10801 tgcgtctatg cgcgcttcgg cgctgcctgt cagacgcagt attagcgtta tcccgacggc
    10861 cagcagcaga ggcgcaacga acatcagcaa ccagaaacgc agaaaagcgg tccggcgttg
    10921 ctgtcgccag ggcaaaaaat taattggcgg attcatcagt acgccttccc aagcgccagc
    10981 cccagcgcga tggcaaagtc tccaccaggc ggcggtagcg gcggctgacg aacagaaacg
    11041 gcctcccagg ggtcaaatcc gccttcgcca catatcgcga cgctgtcggg atcaacggat
    11101 aacgctgccg ccagctcctt cgcgctagtc atccccaccg ccagtttgcg cccccagcta
    11161 tagcgcgtcg cccacagcca ctgttcgtta tcacgccagg ccagacattg ctgatgagaa
    11221 ggtaaaaaag gcaggaatcg ctgtaatgca ctggcatccg gggtgatcgc actaacatga
    11281 acacgcaacc tttctgccag cgtaagcagc gttgccagct ctttgctttg cgcggcagtc
    11341 acgttataag cggggctgag tgagtcttcg ctgtaatcga agcgcaggga gtccggatcc
    11401 atatccagct cgcgggccat cgtccctgac agccaggccg tttgctcccg ctcaccaagg
    11461 gacatcgacg ggcgcggaaa tgaccgctgt aatgtgcgac tggcgggaaa cgccaacata
    11521 atgtgatgac gctgcggcag ttcgcgactc cacggtaaca acgttttagc cagctgctgc
    11581 gcatcaacaa tccgcccatc tttgataatg tcgtgcgcca gcggcaaccg ccaccagcgt
    11641 tgcaaaaagc attcttttgc gccccgcacg atcgcaaccg ctgccgcttc ttgctgttgt
    11701 aaatgcaaac caatttgcca gatcttaaat gtcattgtga tgatctcctt atcacccgtc
    11761 actctgacgg gtatatcaat gcgtctggct tgcctttata ctaccgcgcg tttgtttata
    11821 aactgcccaa atgaaactaa atgggaaatt tccagtgaag ttcgtaaagt attttttgat
    11881 ccttgcagtc tgttgcattc tgctgggagc aggctcgatt tatggcctat accgctacat
    11941 cgagccacaa ctgccggatg tggtgacatt aaaagatgtt cgcctgcaaa ttccgatgca
    12001 gatttacagc gccgatggcg agctgattgc tcaatacggt gagaaacgtc gtattccggt
    12061 tacgttggat caaatcccac cggagatggt gaaagccttt atcgcgacgg aagacagccg
    12121 cttctacgag catcacggcg ttgacccggt ggggatcttc cgtgcggcaa gtgtggcgct
    12181 gttctccggc cacgcgtcac aaggggcgag taccattacc cagcagctgg cgagaaattt
    12241 cttcctcagt ccagaacgca cgatgatgcg taagattaag gaagtcttcc tcgcgattcg
    12301 cattgaacag ttgctgacga aagacgaaat cctcgagctt tatctgaaca agatttacct
    12361 tggttaccgc gcctatggtg tcggtgctgc ggcacaagtc tatttcggta aaacggtcga
    12421 ccaactgacg ctgaacgaaa tggcggtgat agccgggttg ccgaaagcgc cttccacctt
    12481 taatccgctc tactcaatgg atcgtgccgt cgcgcggcgt aacgtcgtac tgtcgcggat
    12541 gctggatgaa gggtatatca cccagcaaca gttcgatcag acacgcactg aggcgattaa
    12601 cgctaattat cacgcgccgg agattgcttt ctctgcgccg tacctgagcg aaatggtgcg
    12661 ccaggagatg tataaccgtt atggcgaaag tgcctatgaa gacggttatc gcatttacac
    12721 caccatcacc cgcaaagtgc agcaggccgc gcagcaggcg gtacgtaata acgtgctgga
    12781 ctacgacatg cgccacggct atcgcggccc ggcaaatgtg ctgtggaaag tgggcgagtc
    12841 ggcgtgggat aacaacaaga ttaccgatac gctgaaggcg ctgccgacct atggtccgct
    12901 gctgcctgcc gcagtcacca gcgccaatcc tcagggagcg acggcgatgc tggcggacgg
    12961 gtcgaccgtc gcattgagta tggaaggcgt tcgctgggcg cgtccttacc gttcggatac
    13021 tcagcaggga ccgacgccgc gtaaagtgac cgatgttctg caaacgggtc agcaaatctg
    13081 ggttcgtcag gttggcgatg catggtggct ggcacaagtg ccggaagtga actcggcgct
    13141 ggtgtcgatc aatccgcaaa atggtgccgt tatggcgctg gtcggtggct ttgatttcaa
    13201 tcagagcaag tttaaccgcg ccacccaggc actgcgtcag gtgggttcca acatcaaacc
    13261 gttcctctac accgcggcga tggataaagg tctgacgctg gcaagtatgt tgaacgatgt
    13321 gccaatttct cgctgggatg caggtgccgg ttctgactgg cagccgaaga actcaccacc
    13381 gcagtatgct ggtccaattc gcttacgtca ggggctgggt cagtcgaaaa acgtggtgat
    13441 ggtacgtgca atgcgggcga tgggcgtcga ctacgctgca gaatatctgc aacgcttcgg
    13501 cttcccggcg caaaacattg tccacaccga atcgctggcg ctgggttcag cgtccttcac
    13561 cccaatgcag gtggcgcgcg gctacgcggt catggcgaac ggcggcttcc tagtggaccc
    13621 gtggtttatc agcaaaattg aaaacgatca gggcggcgtg attttcgaag cgaaaccgaa
    13681 agtagcctgc ccggaatgcg atattccagt gatttacggt gatacgcaga aatcgaacgt
    13741 gctggaaaat aacgatgttg aagatgtcgc tatctcccgc gagcagcaga atgtttctgt
    13801 accaatgccg cagctggagc aggcaaatca ggcgttagtg gcgaagactg gcgcgcagga
    13861 gtacgcaccg cacgtcatca acactccgct ggcattcctg attaagagtg ctttgaacac
    13921 caatatcttt ggtgaaccag gctggcaggg taccggctgg cgtgcaggtc gtgatttgca
    13981 gcgtcgcgat atcggcggga aaaccggtac cactaacagt tcgaaagatg cgtggttctc
    14041 gggttacggt ccgggcgttg tgacctcggt ctggattggc tttgatgatc accgtcgtaa
    14101 tctcggtcat acaacggctt ccggagcgat taaagatcag atctcaggtt acgaaggcgg
    14161 tgccaagagt gcccagcctg catgggacgc ttatatgaaa gccgttcttg aaggtgtgcc
    14221 ggagcagccg ctgacgccgc ctccgggtat tgtgacggtg aatatcgatc gcagcaccgg
    14281 gcagttagct aatggtggca acagccgcga agagtatttc atcgaaggta cgcagccgac
    14341 acaacaggcg gtgcacgagg tgggaacgac cattatcgat aatggtgagg cacaggaatt
    14401 gttctgatta aaaaggcgct tcggcgcctt ttcagttggc tgacaaagtg catttgttta
    14461 tgccggatgc gggtgaacgc gttatccggc taacaaaatc gtgaaaaatc aatatattgc
    14521 aggaacccca taagcctgat aagcgttgtg catcaggcaa acttcacgca tttacactcg
    14581 cccctgccct ttcaaccatt cgcgcacgag gaacagcgcg ctgacattac gcgcttcgtt
    14641 gaagtcaggg tcttccagca aatccatcat atgcgccagc ggccagcgca cctgtggtag
    14701 cggctctggc tcatcgcctt ccagcgattc cgggtagaga tcttgcgcga ccacgatatt
    14761 cattttgctg gaaaagtaag acggtgccat gctgagcttc ttcaaaaaag tcagatcgtt
    14821 cgcaccaaat ccaacctctt cttttagctc gcggttagcg gcttcgtaga cgctttcacc
    14881 cggatcaatt aatcctttcg aaaaacctaa ttcgtaggat tcagttccca ctgcgtattc
    14941 gcggatcagg atcaggtgat cgtccacaat cggcacaatc atcactgctt cccggttggt
    15001 tggacgcatt cgttcataaa cacgccgcac gccattgctg aactccagat ccacgctctc
    15061 gacggtaaac agtcgggaac gggctacagt ttcaacattc agaatggtgg gtttttgtaa
    15121 tgatttgttc atcgtgggat ctatgctgtg aaatcagcgg ttattgtgcg atatcggaca
    15181 cgctttcggc aatgtgaatt gcatgttatt tacatttatg taacttaata aataatcgtc
    15241 ctcaaatcaa attaaaagtc aataggttga aataactcca ggaatttgct gatattccgc
    15301 cttcagaggg tttgctatga tcagcggtta ctgtgatgtg cttaatgatg ctcaagttaa
    15361 gctccacgct tgccgatagc caaccgcaga atcatgtatt gtgtccggtg cgactgacca
    15421 cgcctgacag actaagtaag atggggaaag catgagcacc attgtgattt ttttagctgc
    15481 tttgctggcc tgctcactac ttgcgggatg gctgataaaa gtgcgatcca gacggcgtca
    15541 gctgccctgg accaacgcct tcgcggatgc gcaaacgcgt aaactcacac ctgaagaacg
    15601 tagcgccgtt gaaaattatc ttgagagcct gacgcaggta ttacaggtgc ctggcccaac
    15661 ggaagctagc gcggcaccga tctctctggc gctgaatgcc gaaagcaaca acgtcatgat
    15721 gctgacacac gctatcacac gttacggcat ctctaccgac gatccgaata aatggcgtta
    15781 ctacctcgat tcggtagaag tccacctgcc ccccttctgg gaacagtaca tcaacgatga
    15841 gaataccgtt gaactgattc ataccgattc gctgccgttg gttatttcac tcaacggtca
    15901 tacgctgcag gagtacatgc aggaaacgcg cggctatgcc ttgcaacctg ttccgtccac
    15961 gcaggcgtcg attcgcgggg aagagagtga gcaaatcgag ctactcaata ttcgcaaaga
    16021 aacgcatgaa gaatatgcgc tgagtcgtcc gcgcgggctg cgtgaagcgt tgctgatcgt
    16081 cgcctccttc ctgatgttct ttttctgcct gattaccccg gatgtatttg ttccgtggct
    16141 ggcaggcggc gcattactgc tgctgggcgc aggtctgtgg gggctattcg cgcccccggc
    16201 aaaatcctcc ctgcgagaaa ttcattgtct gcgcggtaca ccccgtcgtt ggggattgtt
    16261 tggcgaaaac gatcaggaac agatcaacaa tatttcactc ggtattatcg acctggtcta
    16321 tcccgcacac tggcagccat acattgctca ggatctcggt caacaaaccg atattgatat
    16381 ctatctcgac cgccacgtag tgcgtcaggg gcgatatctt tcgctgcatg atgaagtaaa
    16441 aaactttccg ttacagcact ggctgcgcag tacgattatc gctgcgggct cgctgctagt
    16501 gctgtttatg ctgttattct ggatcccgct ggatatgccg ctgaaattca ctctctcatg
    16561 gatgaaaggc gcgcagacca ttgaagccac cagcgtaaaa caactggctg acgctggcgt
    16621 gcgggtaggc gatacattgc gtattagcgg tacgggaatg tgtaatattc gcacttccgg
    16681 aacctggagc gcgaaaacca attcaccttt tttaccgttt gactgctcgc agatcatctg
    16741 gaatgacgcc cgctcattac cattaccaga atctgaactg gtcaacaaag cgacggcatt
    16801 gactgaagca gttaatcgcc agctgcaccc taaaccggaa gatgaatctc gcgtcagtgc
    16861 ctcattacgt tcagcgattc aaaaatccgg catggtattg cttgatgatt ttggcgacat
    16921 tgtactgaag acagcggatt tatgttctgc caaagatgac tgtgtgcgac tgaaaaatgc
    16981 gctggtcaat ctcggcaaca gtaaagactg ggacgcgctg gtaaaacgcg ccaacgccgg
    17041 gaagctcgat ggcgtgaatg tgttattacg cccggtgagt gctgaatcgc tggataacct
    17101 ggtggcaacc tccaccgcgc cgtttatcac gcatgaaacg gcgcgagcgg cacaatcact
    17161 aaacagtccg gcccccggcg gattcctgat tgtcagcgac gaaggcagcg attttgttga
    17221 tcagccctgg ccttcggcat cactttacga ctacccgccg caagaacagt ggaacgcttt
    17281 ccagaaactg gcacaaatgc tgatgcatac gccgtttaac gccgaaggta tcgtcacaaa
    17341 aatcttcact gacgccaatg gtacgcagca tattggcctt catccgatcc cggatcgttc
    17401 cggcctgtgg cgctatctca gcaccacatt gctgctactg acgatgctgg gtagcgccat
    17461 ttacaatggc gtacaggcct ggcgtcgtta ccagcgtcat cgcactcgca tgatggagat
    17521 tcaggcctat tatgaaagct gcctgaaccc gcaactgatc accccttcag aaagccttat
    17581 cgaataacac gtttgcgcgg caggttatgc taccctgtcg cgcaaattgc ttcactctgg
    17641 agatttccct catgcatatc aacattgcct ggcaggacgt agataccgtt ctgctggata
    17701 tggacggcac gttgctcgac ctcgccttcg ataactattt ctggcaaaag ctggtgcctg
    17761 aaacatgggg cgcgaaaaac ggggttacgc cacaggaagc gatggaatat atgcgccagc
    17821 aatatcacga cgtacagcat acgctaaact ggtactgtct tgattactgg agtgagcaac
    17881 tgggtctgga tatctgtgcg atgaccaccg agatgggacc gcgcgccgta ctgcgtgaag
    17941 ataccattcc gtttcttgag gcactgaaag ccagcggtaa gcagcgaatt ttgctcacca
    18001 atgctcatcc gcacaacctg gcggtaaaac ttgagcatac gggtctggac gcacaccttg
    18061 atttattact ttccacccac acatttggtt atccgaaaga ggatcagcgg ttatggcatg
    18121 cggtggccga agctacgggt ctgaaagctg aaagaacgct gtttattgat gacagcgaag
    18181 cgattctcga tgctgccgcg caatttggta ttcgttactg cctcggcgtg actaatcctg
    18241 attccgggat tgccgagaaa cagtatcaac gccatccgtc actgaatgac taccgccgcc
    18301 tgatcccctc gctaatgtga aggagacgcc atgaaagaga aacctgctgt tgaggttcga
    18361 ctggataaat ggctatgggc tgcccgtttt tataaaaccc gcgcgctggc ccgtgaaatg
    18421 attgaaggcg gtaaggtgca ttacaacggg cagcgcagca agccgagcaa aatcgtcgag
    18481 ctgaatgcca cgctcactct gcgccaggga aatgacgaac gcacggtgat tgtaaaggcg
    18541 attactgaac agcgtcgccc cgccagcgag gcagccttgc tgtatgaaga gactgcggaa
    18601 agtgtagaga aacgcgaaaa aatggcgatg gcacgtaaac ttaatgcctt aaccatgccg
    18661 cacccggacc gacgcccgga caaaaaagag cgccgcgacc tgttacgatt taaacacggc
    18721 gacagtgaat aactgtcacc tgcaagagag atgattatgc cgcaacatga ccaattacat
    18781 cgctatctgt ttgaaaattt tgccgttcgc ggcgaactgg taaccgtttc ggaaaccctg
    18841 caacagatcc ttgagaacca cgattatccg cagcccgtta aaaacgtgct ggcagaactg
    18901 ctggttgcga ccagcctgtt aaccgctacg ctgaagtttg atggtgatat caccgtacag
    18961 ctgcagggcg acggtccgat gaatctggcg gttattaacg gtaacaataa ccagcaaatg
    19021 cgcggtgtgg cgcgcgtgca gggcgaaatt ccagaaaatg ccgacctgaa aacgctggtc
    19081 ggcaatggtt acgtggtgat caccattacc ccgagcgaag gcgaacgcta tcagggcgta
    19141 gttggtctgg aaggtgatac cctggcggcc tgcctagaag attactttat gcgttctgaa
    19201 cagctgccga cgcgcctgtt tattcgcacc ggcgacgtag acggcaaacc ggctgcaggc
    19261 ggtatgttgt tgcaggtaat gcctgcgcaa aatacccaac aggacgactt tgaccacctg
    19321 gcgacgctaa ccgaaaccat caaaaccgaa gaactgctga ccttaccggc aaacgaagtg
    19381 ttgtggcgtt tgtatcacga agaagaggtg acggtttacg atccgcagga tgtggagttc
    19441 aaatgcacct gctcgcgtga acgttgcgcc gatgcgctga aaacgctgcc tgatgaagaa
    19501 gttgatagca tcctggcgga agatggcgaa attgacatgc attgtgatta ctgcggtaac
    19561 cactatctgt tcaatgcgat ggatattgct gaaatccgca acaacgcgtc tccggcagat
    19621 ccgtaagttc attaatgagt atgtccggca gagagggctc tgccggattt tacgacttaa
    19681 tggcctgatg tgctgcgctt accagcgcct atgaaataaa tcgcaaatta ttttggtcag
    19741 atacagcgtc gtatccgata aaagcgttgt cacttactta agtttatttc cagcgtttca
    19801 ccatctaccg tcagatcgcg ccacgctttc ggcctcgtcc ccagttttcc atccttcgca
    19861 gctgtgagta atttttcttt cgttatttcg ttcggaagta aactcatatc aagcgcgtcc
    19921 cattcttttc tgtctgagtc aaagccatag acaatgactc tgtcatcatt aaaagcaaac
    19981 aggatccgct ccgcctgacc atcactattc agatcttgct cgaccagtac gcaggcgtct
    20041 ttttcaatac aggtcatcac gttatagcga tcctggatta aggccgacca gaatgtcgca
    20101 tccggtttaa cagaaccagg ggcaattaac acattttcgg cgaatacttt ttccgatacc
    20161 tgttcctgaa gatgttgctc tccatctaac gccatcagca gatcccgcgc gcgtttcggg
    20221 tctttcataa acccggcatc gcttttcagc gactcaagcg ccgcacgtcc atagcgaccg
    20281 ctctgttcga gcatgtagat agttacctgg tctggcgtgt ttttgccgct ctgataacgc
    20341 gccatatgac tgttcacgct aatacgcata ctgtccagca ccggcgaatt aagaagcacc
    20401 agtatcacca acaccaataa cgaaaccgca aggttcactt tgccctgaag aacaagggga
    20461 ttttgcccat tacgccagac gatactgaca aaatacccca gcgaccacac cagtaacacc
    20521 agctccgcca gcgcgccctg caagcggtcg acagtccagc cgtactgagc gacccgcagc
    20581 cataaccccc aggtggcgac gaacacatac agcggagcaa ccagcaaagc ggttttaatc
    20641 aggcaacgca acggccccgt ccagggaagt gacgcttttt gcggatcgcg gacaatagcc
    20701 attaagatca attgcaaaaa ggccagcgtt aacagcaacc cggcggcgga gatgtggcga
    20761 gaaatcgcgc tcaggcccgt aaacggcagg gtgatgataa acatcagggt tagcaatgat
    20821 actaacggca gcaaccccgt ggcgattagc gtgaacaact tttgaataga gtcgattaaa
    20881 cgtgactgtg tacgcgcgag gatcaccgcc agtgcggtaa ccagaccaaa tgtaagatac
    20941 ataaaccagt cggttgcaaa aaagagcgta ttgaaaaacg tgatgccaac aagtttaaac
    21001 aactcacccc acagtaaaag caccagccac gtcaggccat tggagagaaa aatcaccagt
    21061 aatataagta cgttatgcca tactgactgg taaaagtagc tatagcggct actgccattg
    21121 cggatacgaa ggctttgttg tatccacggt agcaacaaca ttgccatcaa cagcagatag
    21181 cagccaaaat cccaaatcgc cttttcagct ctccaggggt tcatgccatc agtttgccac
    21241 ttcagccaac cgctcatccc tgtcgtggca ataaacacca gcgccagcca tccccaaagg
    21301 cgcttctgtt taaaagagat cacggagaag agtaaaacgg atgaaaacgc gacggtcgca
    21361 ggcacgccat aaacaatcca actgtgattt tttccggcaa gccaggcgat aagcaggtag
    21421 caaaccagcc cctgaagtaa tccggtagca atcatcccct gaagtaatcc ggtagcaatc
    21481 atcccccagc gtgttgccgg tgaaagttca acgttatcca tgttggttat ccagaatcaa
    21541 aaggtgggtt aattatcgca tccgggcagt agtattttgc ttttttcaga aaataatcaa
    21601 aaaaagttag cgtggtgaat cgatacttta ccggttgaat ttgcatcaat ttcattcagg
    21661 aatgcgattc cactcacaat attcccgcca tataaaccaa gatttaacct tttgagaaca
    21721 ttttccacac ctaaaatgct atttctgcga taatagcaac cgtttcgtga caggaatcac
    21781 ggagtttttt gtcaaatatg aatttctcca gatacgtaaa tctatgagcc ttgtcgcggt
    21841 taacaccccc aaaaagactt tactattcag gcaatacata ttggctaagg agcagtgaaa
    21901 tgcgcgttaa caatggtttg cccccgcaag aactcgaggc ttatggtatc agtgacgtac
    21961 atgatatcgt ttacaaccca agctacgacc tgctgtatca ggaagagctc gatccgagcc
    22021 tgacaggtta tgagcgcggg gtgttaacta atctgggtgc cgttgccgtc gataccggga
    22081 tcttcaccgg tcgttcacca aaagataagt atatcgtccg tgacgatacc actcgcgata
    22141 ctttctggtg ggcagacaaa ggcaaaggta agaacgacaa caaacctctc tctccggaaa
    22201 cctggcagca tctgaaaggc ctggtgacca ggcagctttc cggcaaacgt ctgttcgttg
    22261 tcgacgcttt ctgtggtgcg aacccggata ctcgtctttc cgtccgtttc atcaccgaag
    22321 tggcctggca ggcgcatttt gtcaaaaaca tgtttattcg cccgagcgat gaagagctgg
    22381 caggtttcaa accagacttt atcgttatga acggcgcgaa gtgcactaac ccgcagtgga
    22441 aagaacaggg tctgaactcc gaaaacttcg tggcgtttaa cctgaccgag cgcatgcagc
    22501 tgattggcgg cacctggtac ggcggcgaaa tgaagaaagg gatgttctcg atgatgaact
    22561 acctgctgcc gctgaaaggt atcgcttcta tgcactgctc cgccaacgtt ggcgagaaag
    22621 gcgatgttgc ggtgttcttc ggcctttccg gcaccggtaa aaccaccctt tccaccgacc
    22681 cgaaacgccg cctgattggc gatgacgaac acggctggga cgatgacggc gtgtttaatt
    22741 tcgaaggcgg ctgctacgca aaaactatca agctgtcgaa agaagcggaa cctgaaatct
    22801 acaacgctat ccgtcgtgat gcgctgctgg aaaacgtcac cgtgcgtgaa gatggcacta
    22861 tcgactttga tgatggttca aaaaccgaga acacccgcgt ttcttatcca atctatcaca
    22921 tcgataacat cgttaagccg gtttccaaag cgggccacgc gactaaggtt atcttcctga
    22981 ctgctgatgc tttcggcgtg ttgccgccgg tttctcgcct gactgccgat caaacccagt
    23041 atcacttcct ctctggcttc accgccaaac tggccggtac tgagcgtggc atcaccgaac
    23101 cgacgccaac cttctccgct tgcttcggcg cggcgttcct gtcgctgcac ccgactcagt
    23161 acgcagaagt gctggtgaaa cgtatgcagg cggcgggcgc gcaggcttat ctggttaaca
    23221 ctggctggaa cggcactggc aaacgtatct cgattaaaga tacccgcgcc attatcgacg
    23281 ccatcctcaa cggttcgctg gataatgcag aaaccttcac tctgccgatg tttaacctgg
    23341 cgatcccaac cgaactgccg ggcgtagaca cgaagattct cgatccgcgt aacacctacg
    23401 cttctccgga acagtggcag gaaaaagccg aaaccctggc gaaactgttt atcgacaact
    23461 tcgataaata caccgacacc cctgcgggtg ccgcgctggt agcggctggt ccgaaactgt
    23521 aatgatttga agctggagaa tatctatcca gtatcttata gaaagcaaaa cgggaggcac
    23581 cttcgcctcc cgtttattta cccttctttt gtcgtgccct gcgcccgcgt taccggcact
    23641 ggcagccagg cgcgaatgga aagcccgccc cgctcgctgg tgccaagctc cagcatcccg
    23701 ttatggttat ccacgatacg ctgcacaatt gccagcccta atcccgtgcc gctaatggtg
    23761 cgcgcactgt cgccgcggac aaacggctgg aacaggtgct tacgttgttc cggcgcaatt
    23821 cccggaccgt catcttccac ctggaaccag gcgcgattcg gctccgttcc gctgctgact
    23881 ttgatccagc cattgccata acgggcggcg ttgaccacca tattcgccac cgcgcgtttg
    23941 atcgacagcg ggtgcatttt cacttcaatg ctgccggggt aaagcgcggt ttcaatttcc
    24001 cgctcatagc cactttcggc agcaatcacc tcaccgagca ctgcattaag atccgccatt
    24061 tccatcggca tctcctgccc ggtgcgcagg tagtcgataa actgctcaat gatggcgttg
    24121 cactcttcga tatctttatt gatcgattct gccagatagc catcctgctc gctcatcatc
    24181 tcagtcgcca ggcgaatacg cgtcagcggc gtgcgcaagt cgtgacttac ccccgccatc
    24241 agcagcgtgc ggtcatccgc cagttgctta acaccagccg ccatatggtt aaaggcacgg
    24301 gtaacggaac gcacctccga agcgccatat tcacgcagcg gcggcggaat aatcccttta
    24361 ccaacctgca aggctgcgtg ttcgagatcg accaacggtc ggttctggat acgaataaac
    24421 agccacgccc cgcctatcgc caatagcata atcgccagcg tatagcggaa cagcggagag
    24481 aaatcgccct gatgaatttc ggtcagcggc acgcgtaccc agatattggg cgacagccag
    24541 gttttcagcc agacgacagg cgaacttttg ttgacctcaa cgcgcacttc cgtcgggccg
    24601 cccagttgct gtgccatctg atggcttaag aattcatagt gttgcgccca acgcagacct
    24661 gcctcttcgg cagcctcgtt ggagtagaga gagatcccca gctcacggta gatctcccga
    24721 cggaaagcgg gaggcacaac caactgcgtg ccgtcctcca gttgcagttt gtcggtcatc
    24781 aacatacgca cttcgtacgc gaggacttta ttaaactgct ggaggctcgg caaaatcgcg
    24841 aagttcagca ccaccagata agtcgtcacc aggctggcga acagcaaggt gacgatgagc
    24901 aataacgtac gggcaaatga acttcgtggc gagaagcgca atcgcctcat gctttagagc
    24961 cgtccggtac aaagacgtag cccaggcccc aaacggtctg aatgtaacgc ggatgcgctg
    25021 gatcttcttc taccatgcgg cgcagacgcg aaatctgcac gtcgatggag cgttccattg
    25081 cggaatattc acgaccacgg gcaaggttca tcagcttatc gcgggagagc ggctcacgcg
    25141 gatggctgac cagtgccttc agtaccgcaa actcaccgct ggtgagcggc atcggctcat
    25201 cttcgcggaa catttcgcgc gtaccgaggt taagtttgaa cttaccgaaa gcaattaccg
    25261 cctcttcctg tgacggtgcg cctggcagtt cgttcgcctg acgacgcagc accgcacgaa
    25321 tacgggccag cagttcacgc gggttaaacg gttttggaat gtagtcgtca gcgccaatct
    25381 ccaggcctac gatacggtcc acttcttccc ctttcgccgt caccataatg atcggcatcg
    25441 ggttgctctg actacgaaga cgtcggcaaa tcgacaagcc atcttcacca ggtaacatta
    25501 aatccagtac cataagatgg aaagattcac gagtcagcag gcgatccatc tgttctgcat
    25561 tagcgacgct tcgaacttgg aagccttgtt cggtgagata acgttccagc agcgcacgca
    25621 ggcgcatgtc gtcatcgacc accagaatct tgtagttctc ttgcattgtt tgtactccca
    25681 aaggttcgca acaatttgta agcgtgtatt cttaaaaaag ctcacgttcg tcaccagcta
    25741 aatctggtat gaatttcagc ctaaattgtt acaaagcata ttaaacagca gcttaagtat
    25801 acaatttatt cggcgaaaca ttattgattc tgttgatatg atcacgttat acccaatgtg
    25861 cgcattatca aacagacaaa gggaatcaac gagatgaaaa cgcccctggt tacccgggaa
    25921 gggtatgaaa aactcaaaca agagcttaat tatctctggc gtgaagaacg cccggaggtc
    25981 acaaaaaagg tgacctgggc cgcaagtctg ggcgaccgca gcgaaaatgc tgactatcag
    26041 tataataaaa agcgtctgcg tgaaatcgac cgtcgcgtgc gctatctcac taaatgcctg
    26101 gaaaatctca aaatcgtcga ttactcccct cagcaggaag gcaaagtctt ttttggcgcg
    26161 tgggtggaga ttgaaaacga cgatggcgtg actcaccgtt tccgtattgt cggctacgat
    26221 gaaatttttg gccgtaaaga ttacatctct atcgattccc cgatggcccg cgcattgctg
    26281 aaaaaagaag tcggcgatct ggcggtggtg aatacccctg ccggggaagc gaactggtat
    26341 gttaatgcta tcgagtacgt gaaaccgtaa ggaagagtct taacctcctg ccgatggctg
    26401 gcatttttgc cagccagtcc gtataactat cccctgattt ttgatccgaa aagatgaact
    26461 caaaccatga tgaatgattc gttctgccgc attattgcgg gtgaaattca ggcgcgcccg
    26521 gaacaggttg acgctgccgt tcgcctgctt gacgaaggga ataccgtgcc gtttatcgca
    26581 cgttatcgta aggaaatcac cggcggtctg gatgacacgc agctgcgtaa tctggaaacg
    26641 cgtctgagct atctgcgcga gctggaagag agacgtcagg cgatcctcaa atccatttcc
    26701 gagcaaggca aactcaccga tgatctggcg aaggccatca acgccaccct aagcaaaacc
    26761 gaactcgaag acctctacct gccctacaaa cctaaacgcc gcacccgcgg gcaaatcgcc
    26821 attgaagcag ggcttgagcc gttggctgac ctgctgtgga gcgatccgtc acacacgcca
    26881 gaagtcgccg ctgcacaata tgttgatgcc gataaaggcg tggcagatac caaagccgcg
    26941 ctggatggcg cgcgctatat cctgatggaa cggtttgccg aagatgccgc gctgctggcg
    27001 aaagtgcgtg attatctgtg gaagaacgcg catttggttt ctacggtggt gagcggtaaa
    27061 gaagaggaag gggcgaaatt ccgcgactat ttcgatcatc acgaaccgtt gtccacggtg
    27121 ccttctcacc gcgcgctggc gatgttccgt gggcgtaacg aaggcgtact ccagctttcg
    27181 ctgaatgccg atccgcagtt cgatgagccg cccaaagaga gctattgcga gcaaatcatc
    27241 atggatcacc ttggcctgcg cctgaacaat gccccggcgg atagctggcg caaaagcgta
    27301 gtgagctgga cctggcgcat caaggtgctg atgcatctgg aaaccgaact gatgggtacc
    27361 gtgcgcgaac gcgcggaaga tgaagcaatc aacgtctttg cccgtaacct gcacgatctg
    27421 ctgatggcgg cccctgccgg actgcgtgca acgatgggcc tcgatccggg tctgcgtacc
    27481 ggggtaaaag tggcggtggt cgatgccact ggcaaactgg tggcgaccga taccatttac
    27541 ccgcacaccg gacaagccgc aaaagcagcg atgaccgttg ctgccttgtg tgaaaaacat
    27601 aacgttgaac tggtggcgat cggcaacggt acagcttccc gcgaaactga acgtttctat
    27661 ctcgacgtgc agaagcagtt cccgaaagtg accgcacaga aagtgatcgt cagcgaagct
    27721 ggcgcgtcgg tttactcagc ttccgagctg gcagcgcagg agttcccgga tctcgacgtt
    27781 tcgctgcgtg gtgcggtatc tatcgcccgc cgtttgcagg atccgctggc ggagctggtg
    27841 aaaatcgatc cgaaatctat cggcgtaggt cagtatcagc atgacgtcag ccagacgcaa
    27901 ctggcccgca aactggatgc agtagtagaa gactgcgtaa acgccgttgg cgtcgatctc
    27961 aacaccgctt ctgttccgct attaacccgc gtggcgggcc tgacgcgcat gatggcgcaa
    28021 aacatcgtgg cctggcgcga tgagaacggc cagttccaga accgtcagca actgctgaaa
    28081 gtcagccgtc tggggccgaa agccttcgag cagtgcgcgg gcttcctgcg cattaaccac
    28141 ggtgataacc cgctggacgc ctctaccgtt catccggaag cctatccggt ggtggaacgc
    28201 attctggcag caacacagca ggcactgaaa gatctgatgg gtaacagcag cgaactgcgt
    28261 aacctgaaag cgtctgactt tactgatgaa aaattcggtg tgccgacagt aactgacatc
    28321 atcaaagagc tggaaaaacc gggtcgcgat ccgcgtccgg aatttaaaac cgctcagttt
    28381 gccgatggcg tcgagacaat gaacgacctg caaccgggta tgatcctcga aggcgcagtg
    28441 accaacgtca ccaactttgg cgcgtttgtc gatattggcg tgcatcagga cggcctggtt
    28501 cacatctctt cattgtcgaa caagtttgtg gaagatccgc ataccgtggt gaaagcgggc
    28561 gacattgtga aggtgaaagt gctggaagtg gatcttcagc gtaaacgtat cgccctgact
    28621 atgcgtctgg acgagcagcc tggcgaaacc aactctcgtc gcggcggcgg taatgaacgc
    28681 ccgcaaaaca accgcccggc agccaaacca cgcggtcgtg aagcgcagcc tgccggtaat
    28741 agcgcgatga tggatgcgct ggcggcagcg atgggtaaaa aacgttaaac gcccggactg
    28801 gcccctaaac ctgcgcgata aggcgttcac gccgcatctg gcaacgaacg ctttgcctga
    28861 cataaaagtg ccggagaata tctccggcac ttttattcca cagccaaact cacaatatac
    28921 tccgttaata tttatcactt cattaacaac tgaaacctta attaaacatt agccagtccg
    28981 ggtaactcac tattcgaatt atattttcgc tgcgatataa ccttgagcca catcaacatt
    29041 gagtcagatt attattcaaa ccaacattcg cacacatttt aagtattgct gatagaaacc
    29101 attctcatta tcattgtgtt gttgattatt taatctctcc tttgttggca aatcatctgg
    29161 tctcatgtcg cggtcaaacg ccccatgagg tagttatcca gttaatgaga aacaagtagg
    29221 cacctatgca atacactcca gatactgcgt ggaaaatcac tggcttttcc cgtgaaatca
    29281 gcccggcata tcgccaaaaa ctgctttctc taggcatgtt acctggctcc tcttttgatg
    29341 tggtgcgcgt cgctccactc ggcgacccca ttcatatcga aacccgtcgt gtgagcctgg
    29401 tattacgcaa aaaagatctg gccttattag aagtggaagc ggtttcctgt taatacggtg
    29461 ataacaacaa tgaaaaaatt aaccattggc ttaattggta atccaaattc tggcaagaca
    29521 accttattta accagctcac tggcgcacgt cagcgtgtag gtaactgggc tggcgttacc
    29581 gtcgaacgta aagaagggca attctccacc accgatcatc aggtcacgct ggtggacctg
    29641 cccggcacct attctctgac caccatctca tcgcagacct cgctcgatga gcaaatcgcc
    29701 tgtcactaca ttttgagtgg cgacgccgac ctgctgatta acgtggtgga tgcgtctaac
    29761 cttgagcgta acctgtacct gacgctgcaa ctgctggaac tcggcattcc ctgcatcgtg
    29821 gcgctgaaca tgctcgacat tgccgagaag caaaatattc gtattgaaat tgatgctcta
    29881 tcggcacgtc ttggctgccc ggtgatcccg ctggtttcaa cccgtggtcg cggtattgaa
    29941 gcgctcaagc tggcgattga tcgctataaa gctaacgaga atgtggaact ggtgcattac
    30001 gcacagccgc tactcaacga agcagattca ctggcaaaag tgatgccttc cgacatcccg
    30061 ctgaaacaac gtcgctggct gggcctgcaa atgctggaag gcgatatcta cagccgcgcc
    30121 tacgccggtg aagcgtcgca gcatctggat gccaccctcg cccgtctgcg taatgaaatg
    30181 gatgatccgg cgctgcacat tgccgatgcg cgttaccagt gcattgctgc catctgtgat
    30241 gtggtaagca acaccctgac ggcagaaccc agccgtttca ccactgcggt agataaaatc
    30301 gtgctcaacc gtttcctcgg tctgccgatt ttcctctttg tgatgtacct gatgttcctg
    30361 ctggctatca acatcggcgg ggcgttacag ccgctgttta acgtcggctc cgtggcgcta
    30421 tttgtgcatg gtattcaatg gattggctac acgctccact tcccggactg gctgactatc
    30481 ttcctcgccc agggcctggg tggcggtatt aacaccgtgc tgccactggt gccgcagatc
    30541 ggcatgatgt acctgttcct ctccttcctt gaggactccg gctatatggc gcgtgcagcg
    30601 tttgtgatgg accgcctgat gcaggcgctg ggcttgccgg ggaaatcctt tgtgccgctg
    30661 atcgtcggtt tcggttgtaa cgtaccgtcg gtaatgggcg cacgtacgct tgatgcaccg
    30721 cgtgaacgtc tgatgaccat catgatggca ccgtttatgt cctgcggcgc gcgtctggct
    30781 atcttcgcag tattcgcggc tgccttcttc gggcagaacg gtgccctggc ggttttctcg
    30841 ctgtatatgc tcggtatcgt gatggcggtg ctgactggcc tgatgctcaa gtacaccatc
    30901 atgcgcggtg aagcgacgcc gtttgtcatg gagctgccgg tctaccatgt accacacgtt
    30961 aaaagcctga ttatccagac ctggcagcgt ctgaagggct tcgttctgcg tgctggtaaa
    31021 gtgattatca tcgtcagcat tttcctgagc gctttcaaca gcttctcgtt gagcgggaaa
    31081 atcgtcgata acatcaacga ctcggcgctg gcttccgtca gccgggtgat aacccctgtc
    31141 ttcaagccga ttggcgtaca tgaagataac tggcaggcaa cggttggcct gtttacgggc
    31201 gcaatggcaa aagaagtggt ggtgggtacg ctcaacaccc tctacaccgc agaaaatatt
    31261 caggacgaag agttcaatcc ggcggaattc aacctcggtg aagagctgtt cagtgcggta
    31321 gatgaaacct ggcagagcct gaaagacacc ttcagcctta gcgtactgat gaaccccatt
    31381 gaagctagca aaggcaacgg tgaaatgggt accggggcga tgggcgtgat ggatcagaaa
    31441 ttcggtagcg cagctgccgc atacagctac ctgattttcg tcctgctgta tgtaccatgt
    31501 atctcggtga tgggagccat cgcccgtgaa tcaagccgtg gctggatggg cttctccatc
    31561 ctgtgggggc tgaatatcgc ttactcactg gcaacattgt tctatcaggt cgccagctac
    31621 agtcagcacc caacttacag cctggtgtgc attctggcgg taatcctgtt taacatcgta
    31681 gctatcggtc tgctgcgccg cgcgcgtagc cgggtggata tcgaactgct ggcaacccgc
    31741 aagtcggtaa gcagttgctg cgcagccagc accaccggtg attgccatta atggcttcac
    31801 ttattcaggt acgtgatttg ctggcgttac ggggccgtat ggaagcgacc cagataagcc
    31861 agacattgaa cactccgcag ccaatgatta acgccatgct gcaacagctg gaaagcatgg
    31921 gcaaagccgt gcggattcag gaagaacctg acggctgcct ctctggcagt tgtaaaagct
    31981 gcccggaagg caaagcctgt ctgcgcgagt ggtgggcgct gcgttaacca tactccatcg
    32041 cctgttttga aaagcccggt atgcgtttgc atccgggctt ttttgcgtgc ggctttccat
    32101 aaaaatgcaa ttcttgcagc gcaacgtaag ttcctctgaa agcgtctcgc agggatgaaa
    32161 actcgctaat gcacagatgt ggagtggcgc gtagagtcgc ggcattcaaa caacaggtga
    32221 aggaacgcca tgagtaaaaa gcagagttcc accccacatg atgcgctgtt caaactcttt
    32281 ttacgccaac cggagacggc acgcgatttt cttgcgtttc atttaccggc acccattcac
    32341 gcgctctgtg atatgaaaac cctcaagctg gagtcgagga gctttattga tgacgatctg
    32401 cgtgaaagct attccgatgt gctgtggtcg gtgaaaacgg aacaaggacc aggatacatc
    32461 tattgtctga ttgaacatca aagcacctca aacaaactga tcgcatttcg catgatgcgt
    32521 tacgctattg ccgcaatgca aaatcacctt gatgctggat acaaaacgtt gccgatggtg
    32581 gtgccattgt tgttttacca cggtattgaa agcccctatc cctattcgct gtgttggctg
    32641 gattgtttcg ccgatcccaa actggcaagg cagctttatg cctccgcatt tccgctgatt
    32701 gatgtcaccg tcatgcctga tgatgaaatc atgcagcacc gacgtatggc gctgctggag
    32761 ttaattcaaa aacatattcg tcaacgcgat ctgatggggc tggtagagca aatggcctgc
    32821 ttattaagta gtggatacgc taatgacaga caaatcaaag ggctgtttaa ttacatactg
    32881 caaactggcg acgctgtacg ttttaacgat tttatcgacg gcgttgccga acgttcaccg
    32941 aaacacaagg agagtttaat gactattgcg gaaagattgc ggcaggaggg ggaacaatcc
    33001 aaagccctgc atatagccaa aataatgctt gaatccggag tccctcttgc agacatcatg
    33061 cgatttaccg ggctgtcaga agaagagttg gctgcggcga gt
//
LOCUS       DAZXUK010000018        31186 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZXUK010000018 DAZXUK010000000
VERSION     DAZXUK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31186
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.018"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            389..2047
                     /locus_tag="ACH3L8_000703"
     CDS             389..2047
                     /locus_tag="ACH3L8_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFT4083946.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="ACH3L8_000704"
     CDS             complement(2044..3000)
                     /locus_tag="ACH3L8_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFT4083947.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="ACH3L8_000705"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="ACH3L8_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFT4083948.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="ACH3L8_000706"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="ACH3L8_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFT4083949.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACH3L8_000707"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACH3L8_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFT4083950.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6656..6730
                     /gene="yljB"
                     /locus_tag="ACH3L8_000708"
     CDS             6656..6730
                     /gene="yljB"
                     /locus_tag="ACH3L8_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFT4083951.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="ACH3L8_000709"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="ACH3L8_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFT4083952.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="ACH3L8_000710"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="ACH3L8_000710"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFT4083953.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="ACH3L8_000711"
     tRNA            complement(9859..9946)
                     /locus_tag="ACH3L8_000711"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACH3L8_000712"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACH3L8_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFT4083954.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACH3L8_000713"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACH3L8_000713"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFT4083955.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPDDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACH3L8_000714"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACH3L8_000714"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFT4083956.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACH3L8_000715"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACH3L8_000715"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFT4083957.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACH3L8_000716"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACH3L8_000716"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFT4083958.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="ACH3L8_000717"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="ACH3L8_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFT4083959.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="ACH3L8_000718"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="ACH3L8_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFT4083960.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="ACH3L8_000719"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="ACH3L8_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFT4083961.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="ACH3L8_000720"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="ACH3L8_000720"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFT4083962.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="ACH3L8_000721"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="ACH3L8_000721"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFT4083963.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27412
                     /gene="dmsA"
                     /locus_tag="ACH3L8_000722"
                     /pseudo
     CDS             24969..27412
                     /gene="dmsA"
                     /locus_tag="ACH3L8_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27423..28040
                     /gene="dmsB"
                     /locus_tag="ACH3L8_000723"
     CDS             27423..28040
                     /gene="dmsB"
                     /locus_tag="ACH3L8_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFT4083964.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28042..28833
                     /locus_tag="ACH3L8_000724"
     CDS             28042..28833
                     /locus_tag="ACH3L8_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009390 - dimethyl sulfoxide reductase
                     complex [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFT4083965.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACH3L8_000725"
     CDS             complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACH3L8_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFT4083966.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29810..30958
                     /locus_tag="ACH3L8_000726"
     CDS             29810..30958
                     /locus_tag="ACH3L8_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4083967.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30976..>31186
                     /locus_tag="ACH3L8_000727"
     CDS             30976..>31186
                     /locus_tag="ACH3L8_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFT4083968.1"
                     /translation="MNLLFHTLLFCFFYLTCFFPNFIRLKIKCAAGYNFAFRYFRTSY
                     LPRIIPRFTDAPLILAKDLSMAGNVQ"
BASE COUNT         7689 a         7644 c         8374 g         7479 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgt ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctacctat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacga caataccggc gatgacaatt acgacggcct
    25261 acactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga
    25321 ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat
    25381 tagctgggaa gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta
    25441 cggcaacgag tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg
    25501 ctcctggccg ccgggaaata ccctagtcgc gcgcctgatg aactgctgcg gcggttatct
    25561 taaccattac ggtgactact cttccgcgca aattgcggaa ggtttgaacc atacctacgg
    25621 cggctgggca gatggtaaca gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt
    25681 tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca
    25741 ggcacgccag aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg
    25801 tgccgggcgc gaagatgagt ggatccctat tcgtccagga acagatgccg cactggttaa
    25861 cggtctggcg tacgtcatga tcactaaaaa cctggtggat caggcattcc tcgataaata
    25921 ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa
    25981 agcttatatt ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcgca
    26041 aatcaccggt gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa
    26101 accggcgttt atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac
    26161 ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt ggtattaatg gaggggacag
    26221 cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc
    26281 gatccagacc agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat
    26341 gacggcgctg cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat
    26401 ctggaactat gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga
    26461 aatcctccag gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc
    26521 atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt
    26581 tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa
    26641 accgcgcttt gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg
    26701 cgttgagcaa cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc
    26761 ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat
    26821 ctttaaaaag cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc
    26881 gcaggcaaac ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc
    26941 tgacattgcc gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta
    27001 cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac
    27061 aggtttccac tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc
    27121 ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa
    27181 cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac
    27241 gccacgaatg atgccgggcg tggtcgcact gggtgaaggc gcctggtatg acccggatgc
    27301 aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct
    27361 cgctaagggg aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac
    27421 cgatgacaac ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct
    27481 gcgagctggc ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt
    27541 atgaatatgc tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg
    27601 cctactatct gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc
    27661 cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca
    27721 ttggctgccg ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga
    27781 aaggccatat gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga
    27841 tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta
    27901 aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga
    27961 atattgtgat caaacccaat gccaatagcc gcccgaccgg ggataccact ggctatctgg
    28021 caaacccgaa ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc
    28081 acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc gctgctcaaa
    28141 ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggta
    28201 ctgatgggca ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt
    28261 aactcgctca accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc
    28321 ttttttgctg taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca
    28381 ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg
    28441 gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg
    28501 ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg
    28561 atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca
    28621 gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc
    28681 ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc
    28741 gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg
    28801 catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg
    28861 gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca
    28921 ggaatatggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc
    28981 agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc
    29041 cacgccgaat gccgggtaat ttcattaaag gtccctgatg catcagtcac gacaaagaca
    29101 tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca
    29161 ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta
    29221 tcccaggcgt taatattgcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc
    29281 agttctagaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt
    29341 aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt
    29401 tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca
    29461 tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg
    29521 gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg ggaaatatga
    29581 gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca
    29641 ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata
    29701 taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg
    29761 gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta
    29821 tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt
    29881 gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg
    29941 cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt
    30001 cattaaacgc attggcttta accgcagcta ttatctgtcc tccttcattt ttgctgctgg
    30061 ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc
    30121 gggcgtcggc tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac
    30181 gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt
    30241 tttaggccag ttactggtca gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt
    30301 tacaggtttg acattggcag ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca
    30361 ggctgaaaac catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct
    30421 tggcgtgaat ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc
    30481 tctgtacctc aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact
    30541 ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg
    30601 actgctggtt ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg cgatgcttag
    30661 ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc
    30721 ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca
    30781 agccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta gcgctatgct
    30841 aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta
    30901 tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat
    30961 gaattcaagc aaagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg
    31021 acttgctttt ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt
    31081 gcatttcgct atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc
    31141 cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcagg
//
LOCUS       DAZXUK010000019        30736 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZXUK010000019 DAZXUK010000000
VERSION     DAZXUK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30736
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.019"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACH3L8_000728"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACH3L8_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFT4083969.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="ACH3L8_000729"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="ACH3L8_000729"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFT4083970.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="ACH3L8_000730"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="ACH3L8_000730"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFT4083971.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="ACH3L8_000731"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="ACH3L8_000731"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFT4083972.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="ACH3L8_000732"
     CDS             5718..6659
                     /locus_tag="ACH3L8_000732"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFT4083973.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="ACH3L8_000733"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="ACH3L8_000733"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFT4083974.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="ACH3L8_000734"
     CDS             7679..8929
                     /locus_tag="ACH3L8_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFT4083975.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /locus_tag="ACH3L8_000735"
                     /pseudo
     CDS             complement(9000..9694)
                     /locus_tag="ACH3L8_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="ACH3L8_000736"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="ACH3L8_000736"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFT4083976.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="ACH3L8_000737"
     CDS             10866..12116
                     /locus_tag="ACH3L8_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFT4083977.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="ACH3L8_000738"
     CDS             complement(12172..13260)
                     /locus_tag="ACH3L8_000738"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFT4083978.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACH3L8_000739"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACH3L8_000739"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFT4083979.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="ACH3L8_000740"
     CDS             complement(14194..15243)
                     /locus_tag="ACH3L8_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFT4083980.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /gene="yieE"
                     /locus_tag="ACH3L8_000741"
     CDS             15342..16223
                     /gene="yieE"
                     /locus_tag="ACH3L8_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFT4083981.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="ACH3L8_000742"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="ACH3L8_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFT4083982.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="ACH3L8_000743"
                     /pseudo
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="ACH3L8_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="ACH3L8_000744"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="ACH3L8_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="ACH3L8_000745"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="ACH3L8_000745"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFT4083983.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /gene="yeiB"
                     /locus_tag="ACH3L8_000746"
     CDS             21991..23067
                     /gene="yeiB"
                     /locus_tag="ACH3L8_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFT4083984.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="ACH3L8_000747"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="ACH3L8_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFT4083985.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="ACH3L8_000748"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="ACH3L8_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFT4083986.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="ACH3L8_000749"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="ACH3L8_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFT4083987.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..28134
                     /gene="mglC"
                     /locus_tag="ACH3L8_000750"
     CDS             27124..28134
                     /gene="mglC"
                     /locus_tag="ACH3L8_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFT4083988.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28170..29383)
                     /gene="preA"
                     /locus_tag="ACH3L8_000751"
                     /pseudo
     CDS             complement(28170..29383)
                     /gene="preA"
                     /locus_tag="ACH3L8_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29377..30615)
                     /gene="preT"
                     /locus_tag="ACH3L8_000752"
     CDS             complement(29377..30615)
                     /gene="preT"
                     /locus_tag="ACH3L8_000752"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFT4083989.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT         7450 a         7471 c         8145 g         7670 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacgaatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggcga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatttcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttattcat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggt gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt tggattgata
    18001 attgttatcg tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctagac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggggggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggtggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agacgctgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    27601 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    27661 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    27721 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    27781 gcaaaaatat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc
    27841 gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    27901 ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    27961 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    28021 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    28081 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat
    28141 tgcggatatt gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28201 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28261 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28321 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28381 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28441 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28501 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28561 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28621 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28681 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28741 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28801 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28861 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    28921 gcccgacaat atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga
    28981 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt
    29041 gctgctcatt ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca
    29101 gccgacgcag ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt
    29161 tgaagccgat aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt
    29221 tggcgataaa aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt
    29281 tggcacacat ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa
    29341 agttcacgcc gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcggatct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg
    29941 tagcccggaa ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag
    30001 ttgctccagg gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt
    30061 ttcaatacgg gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc
    30121 gttacgcaac cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg
    30181 gtttgtcagt gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt
    30241 gccgagcgtt ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt
    30301 aacaaaacgc tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc
    30361 gctttggcat aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt
    30421 ttcgcgaatt gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt
    30481 ccccggatcg gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca
    30541 gcgagaggct tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata
    30601 attttgttgc ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata
    30661 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc
    30721 acaaatagct gcggtt
//
LOCUS       DAZXUK010000020        30129 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZXUK010000020 DAZXUK010000000
VERSION     DAZXUK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30129
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.020"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..135
                     /gene="tuf"
                     /locus_tag="ACH3L8_000753"
     CDS             <1..135
                     /gene="tuf"
                     /locus_tag="ACH3L8_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFT4083990.1"
                     /translation="VMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS
                     "
     gene            365..748
                     /gene="secE"
                     /locus_tag="ACH3L8_000754"
     CDS             365..748
                     /gene="secE"
                     /locus_tag="ACH3L8_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFT4083991.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            750..1295
                     /gene="nusG"
                     /locus_tag="ACH3L8_000755"
     CDS             750..1295
                     /gene="nusG"
                     /locus_tag="ACH3L8_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFT4083992.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1454..1882
                     /gene="rplK"
                     /locus_tag="ACH3L8_000756"
     CDS             1454..1882
                     /gene="rplK"
                     /locus_tag="ACH3L8_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFT4083993.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1886..2590
                     /gene="rplA"
                     /locus_tag="ACH3L8_000757"
     CDS             1886..2590
                     /gene="rplA"
                     /locus_tag="ACH3L8_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFT4083994.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3003..3500
                     /gene="rplJ"
                     /locus_tag="ACH3L8_000758"
     CDS             3003..3500
                     /gene="rplJ"
                     /locus_tag="ACH3L8_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFT4083995.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3567..3932
                     /gene="rplL"
                     /locus_tag="ACH3L8_000759"
     CDS             3567..3932
                     /gene="rplL"
                     /locus_tag="ACH3L8_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFT4083996.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4252..8280
                     /gene="rpoB"
                     /locus_tag="ACH3L8_000760"
     CDS             4252..8280
                     /gene="rpoB"
                     /locus_tag="ACH3L8_000760"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFT4083997.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8357..12580
                     /gene="rpoC"
                     /locus_tag="ACH3L8_000761"
     CDS             8357..12580
                     /gene="rpoC"
                     /locus_tag="ACH3L8_000761"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFT4083998.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12822..13955)
                     /gene="thiH"
                     /locus_tag="ACH3L8_000762"
     CDS             complement(12822..13955)
                     /gene="thiH"
                     /locus_tag="ACH3L8_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFT4083999.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13952..14722)
                     /gene="thiG"
                     /locus_tag="ACH3L8_000763"
     CDS             complement(13952..14722)
                     /gene="thiG"
                     /locus_tag="ACH3L8_000763"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFT4084000.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14724..14924)
                     /gene="thiS"
                     /locus_tag="ACH3L8_000764"
     CDS             complement(14724..14924)
                     /gene="thiS"
                     /locus_tag="ACH3L8_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFT4084001.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14908..15663)
                     /gene="thiF"
                     /locus_tag="ACH3L8_000765"
     CDS             complement(14908..15663)
                     /gene="thiF"
                     /locus_tag="ACH3L8_000765"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFT4084002.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15656..16291)
                     /gene="thiE"
                     /locus_tag="ACH3L8_000766"
     CDS             complement(15656..16291)
                     /gene="thiE"
                     /locus_tag="ACH3L8_000766"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFT4084003.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16291..18186)
                     /gene="thiC"
                     /locus_tag="ACH3L8_000767"
     CDS             complement(16291..18186)
                     /gene="thiC"
                     /locus_tag="ACH3L8_000767"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFT4084004.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18267..18367)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18420..18896)
                     /gene="rsd"
                     /locus_tag="ACH3L8_000768"
     CDS             complement(18420..18896)
                     /gene="rsd"
                     /locus_tag="ACH3L8_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFT4084005.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18991..19764
                     /gene="nudC"
                     /locus_tag="ACH3L8_000769"
     CDS             18991..19764
                     /gene="nudC"
                     /locus_tag="ACH3L8_000769"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFT4084006.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19804..20868
                     /gene="hemE"
                     /locus_tag="ACH3L8_000770"
     CDS             19804..20868
                     /gene="hemE"
                     /locus_tag="ACH3L8_000770"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFT4084007.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21025..21696
                     /gene="nfi"
                     /locus_tag="ACH3L8_000771"
     CDS             21025..21696
                     /gene="nfi"
                     /locus_tag="ACH3L8_000771"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFT4084008.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21739..22329
                     /locus_tag="ACH3L8_000772"
     CDS             21739..22329
                     /locus_tag="ACH3L8_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFT4084009.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22516..22788
                     /gene="hupA"
                     /locus_tag="ACH3L8_000773"
     CDS             22516..22788
                     /gene="hupA"
                     /locus_tag="ACH3L8_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFT4084010.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22801..23496
                     /locus_tag="ACH3L8_000774"
     CDS             22801..23496
                     /locus_tag="ACH3L8_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFT4084011.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23498..23923)
                     /gene="zraP"
                     /locus_tag="ACH3L8_000775"
     CDS             complement(23498..23923)
                     /gene="zraP"
                     /locus_tag="ACH3L8_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFT4084012.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24161..25537
                     /gene="zraS"
                     /locus_tag="ACH3L8_000776"
     CDS             24161..25537
                     /gene="zraS"
                     /locus_tag="ACH3L8_000776"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFT4084013.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25534..26859
                     /gene="zraR"
                     /locus_tag="ACH3L8_000777"
     CDS             25534..26859
                     /gene="zraR"
                     /locus_tag="ACH3L8_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFT4084014.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26856..28145)
                     /gene="purD"
                     /locus_tag="ACH3L8_000778"
     CDS             complement(26856..28145)
                     /gene="purD"
                     /locus_tag="ACH3L8_000778"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFT4084015.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28157..29746)
                     /gene="purH"
                     /locus_tag="ACH3L8_000779"
     CDS             complement(28157..29746)
                     /gene="purH"
                     /locus_tag="ACH3L8_000779"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFT4084016.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7052 a         7855 c         8292 g         6930 t
ORIGIN      
        1 gtaatgccgg gcgacaacat caaaatggtt gttaccctga tccacccgat cgcgatggac
       61 gacggtctgc gtttcgcaat ccgtgaaggc ggccgtaccg ttggcgcggg cgttgtagca
      121 aaagttctga gctaattgcc gataacattt gacgcaatgc gcactaaaag ggcatcattt
      181 gatgcccttt ttgcacgctt tcgtaccaga acctggctca tcagtgattt tctttgtcat
      241 aatcattgct gagacaggct ctgttgaggg cgtataatcc gaaaagctaa tacgcgtttc
      301 gatttggttt gcctcgcgat cgcggggtga aaatgtttgt agaaaacttc tgacaggttg
      361 gtttatgagt gcgaataccg aagctcaagg aagcgggcgc ggcctggaag cgatgaagtg
      421 ggtcgttgtg gtggcattgc tcctggcggc gattgtcggc aactaccttt atcgcgacat
      481 tatgctgccg ctgcgtgcgc tggccgtagt aattctgatt gctgcagcgg gtggtgtcgc
      541 gctgttaacg acaaaaggta aagctaccat tgcttttgcc cgtgaagcgc gtaccgaagt
      601 ccgtaaggtc atttggccga ctcgccagga aacattgcac accacgctga ttgtggctgc
      661 ggttaccgca gtaatgtcac tgatcctgtg gggactggat ggtattctgg ttcgcctggt
      721 atcctttatc actggcctga ggttctgaga tgtctgaagc tcctaaaaag cgctggtacg
      781 tcgttcaggc gttttccggt tttgaaggcc gcgtagcaac gtcgctgcgt gagcatatca
      841 aattacacaa catggaagat ttgtttggtg aagtcatggt accaaccgaa gaagtggttg
      901 aaatccgtgg cggtcagcgt cgcaaaagcg aacgtaaatt cttccctggc tacgtcctcg
      961 ttcagatggt gatgaacgac gcgagctggc acttggtgcg cagcgtaccg cgtgtgatgg
     1021 gcttcatcgg cggtacttcc gatcgtcctg cgccaatcag cgataaagaa gtcgatgcga
     1081 ttatgaaccg cctgcagcag gttggtgata agccgcgtcc gaaaacgctg tttgaaccgg
     1141 gtgaaatggt ccgtgttaat gatggtccgt tcgctgactt caacggtgtt gttgaagaag
     1201 tggattacga gaaatctcgt ctgaaagtgt ctgtttctat cttcggtcgt gcgaccccgg
     1261 tagagctgga cttcagccag gttgaaaaag cctaacccag cgatcaaaaa agcggcgatt
     1321 taatcgttgc acaaggcgtg agattggaat acaatttcgc gccttttgtt tttatgggcc
     1381 ttgcccgtaa aacgattttt tatatcacgg ggagcctctc agaggcgtta ttacccaact
     1441 tgaggaattt ataatggcta agaaagtaca agcctatgtc aagctgcagg ttgcagctgg
     1501 tatggctaac ccgagtccgc cagtaggtcc ggctctgggt cagcagggcg taaacatcat
     1561 ggaattctgc aaagcgttca acgcaaaaac tgattccatc gaaaaaggtc tgccgattcc
     1621 ggtagtaatc accgtttacg ctgaccgttc tttcactttc gttaccaaga ccccgccggc
     1681 agcagttctg ctgaaaaaag cggcaggtat caagtctggt tccggtaagc cgaacaaaga
     1741 caaagtgggt aaaatttccc gcgctcagct gcaggaaatc gcgcagacca aagctgccga
     1801 catgactggt gccgacattg aagcgatgac tcgctccatc gaaggtactg cacgttccat
     1861 gggcctggta gtggaggact aagaaatggc taaactgacc aagcgcatgc gtgttatccg
     1921 tgagaaagtt gatgcaacca aacagtacga catcaacgaa gctatcgcac tgctgaaaga
     1981 gctggcgact gctaaattcg tagaaagcgt ggacgtagct gttaacctcg gcatcgacgc
     2041 tcgtaaatct gaccagaacg tacgtggtgc aactgtactg ccgcacggta ctggccgttc
     2101 cgttcgcgta gccgtattta cccaaggtgc aaacgctgaa gctgctaaag ctgcaggcgc
     2161 agaactggta ggtatggaag atctggctga ccagatcaag aaaggcgaaa tgaactttga
     2221 cgttgttatt gcttctccgg atgcaatgcg cgttgttggc cagctgggcc aggttctggg
     2281 tccgcgtggc ctgatgccaa acccgaaagt gggtactgta acaccgaacg ttgctgaagc
     2341 ggttaaaaac gctaaagctg gccaggttcg ttaccgtaac gacaaaaacg gcatcatcca
     2401 caccaccatc ggtaaagtgg actttgacgc tgacaaactg aaagaaaacc tggaagctct
     2461 gctggttgcg ctgaaaaaag caaaaccgac tcaggcgaaa ggcgtgtaca tcaagaaagt
     2521 tagcatctcc accaccatgg gtgcaggtgt tgcagttgac caggctggcc tgagcgcttc
     2581 tgtaaactaa tgcctttacg tgggcggtga ttttgtctac aatcttaccc ccacgtataa
     2641 tgcttaatgc agacgtatat ccgagatatt cgggttgtgg caaggcggca actgagtgag
     2701 tcgccaagag catagctaac tatgtgactg gtgcgaatga aggaagccaa cgccgccaca
     2761 agttgaatag cgacggatag aaaagatttg ttcgttggag cctggcctat ccaggcctcc
     2821 gtcgaagacc gcaggagttt cgcaagaaac ttaatcccct gcgtagacgg tgacagaacg
     2881 ctaagattat tcttttatat tctggcttgt ttctgctcac cgtaattaag acgctctctc
     2941 cgtttggagg agtgaagtga gttccagaga ttttctctgg caaacatcca ggagcaaagc
     3001 taatggcttt aaatcttcaa gacaaacaag cgattgttgc tgaagtcagc gaagtagcca
     3061 aaggcgcgct gtctgcagta gttgcggatt cccgtggcgt aactgtagat aaaatgactg
     3121 aactgcgtaa agcaggtcgc gaagctggcg tatacatgcg tgttgttcgt aacaccctgc
     3181 tgcgccgtgc tgttgaaggt actccgttcg agtgcctgaa agacgcgttt gttggtccga
     3241 ccctgattgc atactctatg gaacacccgg gcgctgctgc tcgtctgttc aaagagttcg
     3301 cgaaagcgaa tgcaaaattt gaggtcaaag ccgctgcctt tgaaggtgag ctgatcccgg
     3361 cgtctcagat cgaccgcctg gcaactctgc cgacctacga agaagcaatt gcacgcctga
     3421 tggcaaccat gaaagaagct tcggctggca aactggttcg tactctggct gctgtacgcg
     3481 atgcgaaaga agctgcttaa tcgcagttat ctttttaacg cattcgctta cgtataaact
     3541 tattctgata ttcaggaaca atttaaatgt ctatcactaa agatcaaatc attgaagcag
     3601 ttgcagctat gtctgtaatg gacgttgtag aactgatctc tgcaatggaa gaaaaattcg
     3661 gtgtttccgc tgctgctgct gtagctgtag ctgctggccc ggttgaagct gctgaagaaa
     3721 aaactgaatt cgacgtaatt ctgaaagctg ctggcgctaa caaagttgct gttatcaaag
     3781 cagtacgtgg cgcaactggc ctgggtctga aagaagctaa agacctggta gaatctgcac
     3841 cggctgctct gaaagaaggc gtgagcaaag acgacgcaga agcactgaaa aaagctctgg
     3901 aagaagctgg cgctgaagtt gaagttaaat aagccaaccc ttccggttgc agcctgagaa
     3961 atcaggctga tggctggtga ctttttagtc accagccttt ttgcgctgta aggcgccagt
     4021 agcgtttcac actgtttgac tactgctgtg cctttcaatg cttgtttcta tcgacgactt
     4081 aatatactgc gacaggacgt ccgttctgtg taaatcgcaa tgaaatggtt taagcgtgat
     4141 agcaacaggc attgcggaaa gtgttccatt ttccggtcaa caaaatagtg ttgcacaaac
     4201 tgtccgctca atggacagat gggtcgactt gtcagcgagc tgaggaaccc tatggtttac
     4261 tcctataccg agaaaaaacg tattcgtaag gattttggta aacgtccaca agttctggat
     4321 gtaccttatc tcctttctat ccagcttgac tcgtttcaga aatttatcga gcaagatcct
     4381 gaagggcagt atggtctgga agctgctttc cgttccgtat tcccgattca gagctacagc
     4441 ggtaattccg agctgcaata cgtcagctac cgccttggcg aaccggtgtt tgacgtccag
     4501 gaatgtcaaa tccgtggcgt gacctattcc gcaccgctgc gcgttaaact gcgtctggtg
     4561 atctatgagc gcgaagcgcc ggaaggcacc gtaaaagaca ttaaagaaca agaagtctac
     4621 atgggcgaaa ttccgctcat gacagacaac ggtacctttg ttatcaacgg tactgagcgt
     4681 gttatcgttt cccagctgca ccgtagtccg ggcgtcttct ttgactccga caaaggtaaa
     4741 acccactctt cgggtaaagt gctgtataac gcgcgcatca tcccttaccg tggttcctgg
     4801 ctggacttcg aattcgatcc gaaggacaac ctgttcgtac gtatcgaccg tcgccgtaaa
     4861 ctgcctgcga ccatcattct gcgcgccctg aactacacca cagagcagat cctcgacctg
     4921 ttctttgaaa aagttatctt tgaaatccgt gataacaagc tgcagatgga actggtgccg
     4981 gaacgcctgc gtggtgaaac cgcatctttt gacatcgaag ctaacggtaa agtgtacgta
     5041 gaaaaaggcc gccgtatcac tgcgcgccac attcgccagc tggaaaaaga cgacgtcaaa
     5101 ctgatcgaag tcccggttga gtacatcgca ggtaaagtgg ttgctaaaga ctatattgat
     5161 gagtctaccg gcgagctgat ctgcgcagcg aacatggagc tgagcctgga tctgctggct
     5221 aagctgagcc agtctggtca caagcgtatc gaaacgctgt tcaccaacga tctggatcac
     5281 ggcccatata tctctgaaac cttacgtgtc gacccaacta acgaccgtct gagcgcactg
     5341 gtagaaatct accgcatgat gcgccctggc gagccgccga ctcgtgaagc agctgaaagc
     5401 ctgttcgaga acctgttctt ctccgaagac cgttatgact tgtctgcggt tggtcgtatg
     5461 aagttcaacc gttctctgct gcgcgaagaa atcgaaggtt ccggtattct gagcaaagac
     5521 gacatcattg atgttatgaa aaagctcatc gatatccgta acggtaaagg cgaagtcgat
     5581 gatatcgacc acctcggcaa ccgtcgtatc cgttccgttg gcgaaatggc ggaaaaccag
     5641 ttccgcgttg gcctggtacg tgtagagcgt gcggtgaaag agcgtctgtc tctgggcgat
     5701 ctggataccc tgatgcctca ggatatgatc aacgccaagc cgatttccgc agcagtgaaa
     5761 gagttcttcg gttccagcca gctgtctcag tttatggacc agaacaaccc gctgtctgag
     5821 attacgcaca aacgtcgtat ctccgcactc ggcccaggcg gtctgacccg tgaacgtgca
     5881 ggcttcgaag ttcgagacgt acacccgact cactacggtc gcgtatgtcc aatcgaaacc
     5941 cctgaaggtc cgaacatcgg tctgatcaac tctctgtccg tgtacgcaca gactaacgaa
     6001 tacggcttcc ttgagactcc gtatcgtaaa gtgaccgacg gtgttgtaac tgacgaaatt
     6061 cactacctgt ctgctatcga agaaggcaac tacgttatcg cccaggcgaa ctccaacctg
     6121 gatgaagaag gccacttcgt agaagacctg gtaacttgcc gtagcaaagg cgaatccagc
     6181 ttgttcagcc gcgaccaggt tgactacatg gacgtatcca cccagcaggt ggtatccgtc
     6241 ggtgcgtccc tgatcccgtt cctggaacac gatgacgcca accgtgcatt gatgggtgcg
     6301 aatatgcaac gtcaggccgt tccgactctg cgcgctgata agccgctggt tggtactggt
     6361 atggaacgtg ctgttgccgt tgactccggt gtaactgcgg tagctaaacg tggtggtgtc
     6421 gttcagtacg tggatgcttc ccgtatcgtt atcaaagtta acgaagacga gatgtatccg
     6481 ggtgaagcag gtatcgacat ctacaacctg accaaataca cccgttctaa ccagaacacc
     6541 tgtatcaacc agatgccgtg tgtgtctctg ggtgaaccgg ttgaacgtgg cgacgtgctg
     6601 gcagacggtc cgtccaccga cctcggtgaa ctggcgcttg gtcagaacat gcgcgtagcg
     6661 ttcatgccgt ggaatggtta caacttcgaa gactccatcc tcgtatccga gcgtgttgtt
     6721 caggaagacc gtttcaccac catccacatt caggaactgg cgtgtgtgtc ccgtgacacc
     6781 aagctgggtc cggaagagat caccgctgac atcccgaacg tgggtgaagc tgcgctctcc
     6841 aaactggatg aatccggtat cgtttacatt ggtgcggaag tgaccggtgg cgacattctg
     6901 gttggtaagg taacgccgaa aggtgaaact cagctgaccc cagaagaaaa actgctgcgt
     6961 gcgatcttcg gtgagaaagc ctctgacgtt aaagactctt ctctgcgcgt accaaacggt
     7021 gtatccggta cggttataga cgttcaggtc tttactcgcg atggcgtaga aaaagacaaa
     7081 cgtgcgctgg aaatcgaaga aatgcagctc aaacaggcga agaaagacct gtctgaagaa
     7141 ctgcagatcc tcgaagcggg tctgttcagc cgtatccgtg ctgtgctggt agccggtggc
     7201 gttgaagctg agaagctcga caaactgccg cgcgatcgct ggctggagct gggcctgaca
     7261 gacgaagaga aacaaaatca gctggaacag ctggctgagc agtatgacga actgaaacac
     7321 gagttcgaga agaaactcga agcgaaacgc cgcaaaatca cccagggcga cgatctggca
     7381 ccgggcgtgc tgaagattgt taaggtatat ctggcggtta aacgccgtat ccagcctggt
     7441 gacaagatgg caggtcgtca cggtaacaag ggtgtaattt ctaagatcaa cccgatcgaa
     7501 gatatgcctt acgatgaaaa cggtacgccg gtagacatcg tactgaaccc gctgggcgta
     7561 ccgtctcgta tgaacatcgg tcagatcctc gaaacccacc tgggtatggc tgcgaaaggt
     7621 atcggcgaca agatcaacgc catgctgaaa cagcagcaag aagtcgcgaa actgcgcgaa
     7681 ttcatccagc gtgcgtacga tctgggcgct gacgttcgtc agaaagttga cctgagtacc
     7741 ttcagcgatg aagaagttat gcgtctggct gaaaacctgc gcaaaggtat gccaatcgca
     7801 acgccggtgt tcgacggtgc gaaagaagca gaaattaaag agctgctgaa acttggcgac
     7861 ctgccgactt ccggtcagat ccgcctgtac gatggtcgca ctggtgaaca gttcgagcgt
     7921 ccggtaaccg ttggttacat gtacatgctg aaactgaacc acctggtcga cgacaagatg
     7981 cacgcgcgtt ccaccggttc ttacagcctg gttactcagc agccgctggg tggtaaggca
     8041 cagttcggtg gtcagcgttt cggggagatg gaagtgtggg cgctggaagc atacggcgca
     8101 gcatacaccc tgcaggaaat gctcactgtt aagtctgatg acgtgaacgg tcgtaccaag
     8161 atgtataaaa acattgtgga cggcaaccat cagatggagc cgggcatgcc agaatccttc
     8221 aacgtattgt tgaaagagat tcgttcgctg ggtatcaaca tcgaactgga agacgagtaa
     8281 ttctcgctca aacaggtcac tgctgtcggg gtaaaacccg gcagcggatt gtgctaactc
     8341 cgacgggagc aaatccgtga aagatttatt aaagtttctg aaagcgcaga ctaaaaccga
     8401 agagtttgat gcgatcaaaa ttgctctggc ttcgccagac atgatccgtt catggtcttt
     8461 cggtgaagtt aaaaagccgg aaaccatcaa ctaccgtacg ttcaaaccag aacgtgacgg
     8521 ccttttctgc gcccgtatct ttgggccggt aaaagattac gagtgcctgt gcggtaagta
     8581 caagcgcctg aaacaccgtg gcgtcatctg tgagaagtgc ggcgttgaag tgacccagac
     8641 taaagtacgc cgtgagcgta tgggccacat cgaactggct tccccgactg cgcacatctg
     8701 gttcctgaaa tcgctgccgt cccgtatcgg tctgctgctc gatatgccgc tgcgcgatat
     8761 cgaacgcgta ctgtactttg aatcctatgt ggttatcgaa ggcggtatga ccaacctgga
     8821 acgtcagcag atcctgactg aagagcagta tctggacgcg ctggaagagt tcggtgacga
     8881 attcgacgcg aagatgggtg cggaagcaat ccaggctctg ctgaagagca tggatctgga
     8941 gcaagagtgc gaacagctgc gtgaagagct gaacgaaacc aactccgaaa ccaagcgtaa
     9001 aaagctgacc aagcgtatca aactgctgga agcgttcgtt cagtctggta acaaaccaga
     9061 gtggatgatc ctgaccgttc tgccggtact gccgccagat ctgcgtccgc tggttccgct
     9121 ggatggtggt cgtttcgcga cttctgacct gaacgatctg tatcgtcgcg tcattaaccg
     9181 taacaaccgt ctgaaacgtc tgctggatct ggctgcgccg gacatcatcg tacgtaacga
     9241 aaaacgtatg ctgcaggaag cggtagacgc cctgctggat aacggtcgtc gcggtcgtgc
     9301 gatcaccggt tctaacaagc gtcctctgaa atctttggcc gacatgatca aaggtaaaca
     9361 gggtcgtttc cgtcagaacc tgctcggtaa gcgtgttgac tactccggtc gttctgtaat
     9421 caccgtaggt ccatacctgc gtctgcatca gtgcggtctg ccgaagaaaa tggcgctgga
     9481 gctgttcaaa ccgttcatct acggcaagct ggaactgcgt ggtcttgcta ccaccattaa
     9541 agctgcgaag aaaatggttg agcgcgaaga agctgtcgtt tgggatatcc tggacgaagt
     9601 tatccgcgaa cacccggtac tgctgaaccg tgcaccgact ctgcaccgtc tgggtatcca
     9661 ggcatttgaa ccggtactga tcgaaggtaa agctatccag ctgcacccgc tggtttgtgc
     9721 ggcatataac gccgacttcg atggtgacca gatggctgtt cacgtaccgc tgacgctgga
     9781 agcccagctg gaagcgcgtg cgctgatgat gtctaccaac aacatcctgt ccccggcgaa
     9841 cggcgaacca atcatcgttc cgtctcagga cgttgtactg ggtctgtact acatgacccg
     9901 tgactgtgtt aacgccaaag gcgaaggcat ggtgctgact ggcccgaaag aagcagaacg
     9961 tctgtatcgc tctggtctgg cttctctgca tgcgcgcgtt aaagtgcgta tcaccgagta
    10021 tgaaaaagat gctaacggtg aattagtagc gaaaaccagc ctgaaagaca cgactgttgg
    10081 ccgtgccatt ctgtggatga ttgtaccgaa aggtctgcct tactccatcg tcaaccaggc
    10141 gctgggtaaa aaagcaatct ccaaaatgct gaacacctgc taccgcattc tcggtctgaa
    10201 accgaccgtt atttttgcgg accagatcat gtacaccggc ttcgcctatg cagcgcgttc
    10261 tggtgcatct gttggcatcg atgacatggt catcccggag aagaaacacg aaatcatctc
    10321 cgaggcagaa gcagaagttg ctgaaattca ggagcagttc cagtctggtc tggtaactgc
    10381 gggcgaacgc tacaacaaag ttatcgatat ctgggctgcg gcgaacgatc gtgtatccaa
    10441 agcgatgatg gataacctgc aaactgaaac cgttattaac cgtgacggtc aggaagagaa
    10501 gcaggtttcc ttcaacagca tctacatgat ggccgactcc ggtgcgcgtg gttctgcggc
    10561 acagattcgt cagcttgctg gtatgcgtgg tctgatggca aagccggatg gctccatcat
    10621 cgaaacgcca atcaccgcga acttccgtga aggtctgaac gtactccagt acttcatctc
    10681 cacccacggt gctcgtaaag gtctggcgga taccgcactg aaaactgcga actccggtta
    10741 cctgactcgt cgtctggttg acgtggcgca ggacctggtg gttaccgaag acgattgtgg
    10801 tacccatgaa ggtatcatga tgactccggt tatcgagggg ggtgacgtta aagagccgct
    10861 gcgcgatcgc gtactgggtc gtgtaactgc tgaagacgtt ctgaagccgg gtactgctga
    10921 tattctcgtt ccgcgcaaca cgctgctgca cgaacagtgg tgtgacctgc tggaagagaa
    10981 ctctgtcgac gcggtaaaag tacgttctgt tgtatcttgt gacaccgact ttggtgtatg
    11041 tgcgcactgc tacggtcgtg acctggcgcg tggccacatc atcaacaaag gtgaagcaat
    11101 cggtgttatc gcggcacagt ccatcggtga accgggtaca cagctgacca tgcgtacgtt
    11161 ccacatcggt ggtgcggcat ctcgtgcggc tgctgaatcc agcatccagg tgaaaaacaa
    11221 aggtagcatc aagctcagca acgtgaagtc ggttgtgaac tccagcggta aactggttat
    11281 cacttcccgt aacaccgaac tgaaactgat cgacgaattc ggtcgtacca aagaaagcta
    11341 caaagtgcct tacggtgcgg tactggcgaa aggcgatggc gaacaggttg ctggcggcga
    11401 aaccgttgca aactgggacc cgcacaccat gccggttatc accgaagtaa gcggttttgt
    11461 acgctttact gacatgatcg acggccagac cattactcgt cagaccgacg aactgaccgg
    11521 tctgtcttcg ctggtggttc tggattccgc agaacgtacc gcaggtggta aagatctgcg
    11581 tccggcactg aaaatcgttg atgctcaggg taacgacgtt ctgatcccag gtaccgatat
    11641 gcctgcgcag tacttcctgc cgggtaaagc gattgttcag ctggaagatg gcgtacagat
    11701 cagctctggt gacaccttgg cgcgtattcc gcaggaatcc ggcggtacca aggacatcac
    11761 cggtggtctg ccgcgcgttg cggacctgtt cgaagctcgt cgtccgaaag agccggcaat
    11821 cctggctgaa atcagcggta tcgtttcctt cggtaaagaa accaaaggta aacgtcgtct
    11881 ggttatcacc ccggtagacg gtagcgatcc gtacgaagag atgattccga aatggcgtca
    11941 gctcaacgtg ttcgaaggtg aacgtgtaga acgtggtgac gtaatttccg acggtccgga
    12001 agcgccgcac gacattctgc gtctgcgtgg tgttcatgct gtgactcgtt acatcgttaa
    12061 cgaagtacag gacgtatacc gtctgcaggg cgtaaagatt aacgataaac acatcgaagt
    12121 tatcgttcgt cagatgctgc gtaaagctac catcgttaac gcgggcagct ccgacttcct
    12181 ggaaggcgaa caggttgaat actctcgcgt caagatcgca aaccgcgaac tggaagcgaa
    12241 cggcaaagtg ggtgcaactt actcccgcga tctgctgggt atcaccaaag cgtctctggc
    12301 aaccgagtcc ttcatctccg cggcatcgtt ccaggagacc actcgcgtgc tgaccgaagc
    12361 agccgttgcg ggcaaacgcg acgaactgcg cggcctgaaa gagaacgtta tcgtgggtcg
    12421 tctgatcccg gcaggtaccg gttacgcgta ccaccaggat cgtatgcgtc gccgtgctgc
    12481 gggtgaagct ccggctgcac cgcaggtgac tgcagaagac gcatctgcca gcctggcaga
    12541 actgctgaac gcaggtctgg gcggttctga taacgagtaa tcgttaatcc gcaaataacg
    12601 taaaaacccg cttcggcggg tttttttatg ggtgttattc agagggcaaa ttcttcacgg
    12661 aaaatattaa gaaaggcagg gcgaaacgag gaagaagctt ttcgccctgg acaacattcc
    12721 tgctgacggc actaccataa aaaaacacaa cgctttcaac aaccttgcgt ttttcatctt
    12781 ccgtagcggc tcggaataaa tttacgttct tacattccgt ctcatagtct ttgcgaggcg
    12841 cgtcccagat agctgtccca gtctttccat accggctgca aaccctgagc ggttaacacg
    12901 gcagcaaccg cttccggtct gcgatcgtcg tgcggtgaga actgttccaa ctcggggtga
    12961 ttatcggcat agccacctgg ctgcgttttc gagaaggcgc tgacgttatt gatcgccagc
    13021 ggaataacgc gatcgcgaaa ccacggtgat tcccgcgtgg agagtgacag ttcaatctcc
    13081 ggtgcaagca gtcggaaggc gcagatggtt tgcactaact ggcgttcatc cataatcgac
    13141 gcaggctcaa tgccgccagt acacgggcgc aggcgcggaa aggagacaga gtaacggctt
    13201 tgccagtaat gctgttgcag ccatagcaaa tgttcggcaa ccatatagca gtcaacgcgc
    13261 cagttgtcgg aaaggccaat tagcgcgccg aggcctatct tatcaatccc cgcacgcccc
    13321 agccgatccg gcgtttccag ccgccagaag aagtcctgtt ttttgccttt caaatgatgg
    13381 cgggtataag tcgcctcgtg atatgtctcc tgataaacca tcaccccatc cagaccaagt
    13441 tgctttaact cggcgtattc cgtctccgcc agcggttgca cttccatttg tagtgaagag
    13501 aactgttcac gcagggcggg gagatgacga cgaaagtaat ccatccccac tttcgcctga
    13561 tgttcaccag tgactaatag cagatgttca aagcccatct cccgtatagc ggcgctttcc
    13621 ctggcaatat ccgcttcatc cagcgttttg cgcttgatgc gattactcat ggaaaatccg
    13681 cagtacgtgc agtcgttagc gcaaagattg gaaagataaa gcgggacgta gaaactaact
    13741 gtgttgccaa atcgctgacg ggtcagacgc tgcgcctgtt gggccagttg ttccagatag
    13801 ccactggcgg caggcgataa cagcgccatc atgtcgtcgc gggtgagttg cgaggcattt
    13861 agcgcccgct ctacgtcagc agccgttttg ccgttgatac gcaggcggat gtcgtcccag
    13921 tccagttgtc gccagcgatt gctgaaggtt ttcatgccga tgcctccaga aatccggtca
    13981 gcgggctggt ggcatgagca aaataactgc ggctgcccgg tccggactga cgtgccagca
    14041 ggcctacttc taccgccaga cgaaatgcct tcgccatgtt gacgggatcg tccgcgacgg
    14101 caatcgccgt attcactaac accgcgtcgg cccccatttc cagcgcctgc gtggcatggc
    14161 tgggaacgcc gatgccagca tcgacaacca ccggaactgt ggcctgctgg ataataatct
    14221 ccagcatggc acgggtttcc agtccctgat tcgagccaat cggcgcgccg agcggcatca
    14281 ccgctgcaca gccgacttct tccagacgtt tacacaatac cggatcggcc ccgcagtaag
    14341 gcagcacgac aaatccctgt tgtaccagcg tttcggcggc tttcagggtt tcgatgggat
    14401 cgggcaacag ccagcgggcg tcagggtgaa tctccaattt tagccagttt gtgcctaacg
    14461 cttcacgtgc cagatgggcg gcgaaaatgg cttcttccgc tgttttcgcc ccggaagtat
    14521 tcggcagcag ggccacaccc gccgcgataa gcggttcgag gatagcgtcg ttgtgctggc
    14581 gcaagtcgac acgtttcatc gccagtgtca ccagctggct gccggaagcg cggatcgcct
    14641 ccaccatcag ttgtgaagaa gcgaatttcc ctgtgccggt aaacagatgt gaatcaaacg
    14701 ttttgtccgc aatacgtaac atctcaaccc cctgcaataa cctgaaaaag caggatctgg
    14761 tcgccatcct gcacgatatg ttgcgtccac tgctcacgcg ggacgatttg ctgattaatc
    14821 gccagagccg cgcccgcttg tcgttggtcg agttgctcca gtagttcgtg aacagtttgc
    14881 ccggcggcgc actgcatcgg ttgatcgtta aacaggatct gcattgcttc ctccgcatac
    14941 cgggcaacca ctggcgcggc gcaacgccag gctgcgccac tggctcgact taccgtcgaa
    15001 cagtcggagt tctcccgcag gcgtctctat accgcttaat aacttaatgg cttccagtgc
    15061 ctgcaaagtg cccatcaccc cgaccaccgg gccaaccacg cccgccgtgc ggcagttgcg
    15121 ttctggctgc tggttatctg gccacaggca gcggtaacac ccctgctccc agggcggtgt
    15181 cagtaccatc aactgaccgc caaatccgac cgcgctggcg gtgataagcg gcgtgttgag
    15241 tgccacgcag gcggcattaa tctcctggcg agtcgccata ttgtcggtac agtcgagcac
    15301 cacatcggct tgtgcaaccg catctttcaa cgcctcaccc gttaaccgtt gttgtaatgc
    15361 tgtcagttga atgtcgggat tcaactgtgt cagtcgctgt tggctgacct gcgatttcgg
    15421 gctgttgata tcttcagtgg taaagaggat ttgtcgttgc agattgctta aatgcacatc
    15481 gtcgtcatct gccagtacca gcgtcccgac gccagcccct gccaggtata gcgcagcagg
    15541 tgtacccagc ccgccaagac caataatcag cacctggctg tcgagcagtt tttgctgccc
    15601 gtcaagagcg atatcgtcga gcagaatttg gcggctataa cgcataaagt cacggtcatt
    15661 catcgccaac tcctgcaatt tccagcaact gtgccgttgc caaacgccag tctgcggctt
    15721 gagtaatggc gctgacgacg gcgatactgc cgacacccgt tgctatcacc gcaggcgcgc
    15781 gtgccagact gataccgcca atcgccacgg tgggataatc cgccaggcgc tcaacatgcc
    15841 gtgccaactg ttccagcccc tgcggtgcag aaggcatctg tttggtttgc gtcgggaaca
    15901 catgtcccag cgcgatataa gaggggcgtg ctgccagcgc gacgtcgatt tccatatcgt
    15961 catgtgtcga aacgcccagc cgcaggcctg ccgcgcggat ggcattgaga tcggtggctt
    16021 gcaaatcttc ctgccccaaa tggacgccat acgcctgatg cttgatcgcg agccgccagt
    16081 aatcgttgat aaacaatcgc gcgttatagc ggcggcccag cgcaattgcc gccacgacat
    16141 cggcttccac ctcttcatcg cgccgatctt tgatgcgtag ctggagagta cgtacgcctg
    16201 catccaacag acgttcgatc cactgtacgc tgtccaccac cgggtacagt cctaaacgaa
    16261 aaggtacagg aggaaaatca ggctgataca tcacgcttcc tccttatgca ggtagatttc
    16321 tccgcctctg gcacggaagt tctccgacat atccgccatt cccacttcaa tggtttgcgc
    16381 ggcggcgtaa tcgcgtactt cctggctgat tttcatcgag cagaattttg gcccacacat
    16441 ggagcaaaaa tgggcgactt tgccggactc ttggggcagg gtttcatcgt gataagcgcg
    16501 ggcggtaaac gggtcgaggg ccagattaaa ctggtcttcc cagcgaaatt cgaagcgggc
    16561 tttcgacatg gcgttatcgc gaatttgcgc gcccggatgc ccttttgcca ggtcagcagc
    16621 gtgggcggca atcttataag tgataagccc ctgcttaaca tcttctttat tgggcagacc
    16681 cagatgctct tttggcgtta cgtaacagag catcgcgcag ccaaaccagc caatcatcgc
    16741 cgcaccaatc cccgacgtga agtggtcata gcccggtgca atatcggtag ttagcggccc
    16801 cagagtgtaa aacggcgctt cgtggcagtg ctctaactcc tcggtcatat tgcggcggat
    16861 catctgcatc ggcacatggc ctgggccttc aatcatcacc tgtacgtcat attcccaggc
    16921 gattttggtc agttcgccca gcgtatgcaa ttcggcaaac tgcgcttcat cgttggcgtc
    16981 ctgaatagaa ccggggcgca gaccgtcgcc cagcgacagc gaaacgtcat aagcggcaca
    17041 gatttcacaa atttcgcgga agtgttgata gaggaaattt tcctgatgat gggagaggca
    17101 ccatttcgcc ataatcgaac cgccgcgaga gacgataccg gtcaggcgtt tcgcggtcat
    17161 cggcacatag cgcagcagta cgcccgcatg gatagtgaag taatccacac cttgctcggc
    17221 ctgttccagc agcgtgtcgc ggaacgcttc ccaggtaaga tcttcggcga tcccgttaac
    17281 cttctccagc gcctggtaga tcggcactgt accgatcggc accggactgt tacgcaaaat
    17341 ccactcgcgg gtttcgtgaa tatagcgacc ggtggagaga tccatcaccg tatccgctcc
    17401 ccagcgcgtg gaccatacca gcttttccac ttcttcttcg atggaagagg tgaccgccga
    17461 gttgccgata ttggcgttaa cttttaccag gaagttacgg ccaataatca tcggctccga
    17521 ttccggatga ttaatgttgg ccgggataat cgcacgtccg gcagcaactt catcacggac
    17581 aaattccgca gtgatatttt ccggcagatg tgcgccaaag ctcattcccg gatgctggtg
    17641 gcgtaaaacc tcgctacgga tgcgctcgcg gcccatattc tcgcggatgg cgatgaattc
    17701 catttccggc gtgatgatgc cctggcgggc gtagtgcagt tgggtgacac ggcgtcctgt
    17761 tttggcgcgt tttggcgtta ataagccgct aaaacgtagc tcgtcgagac catcatctgc
    17821 cagccgcgtt ctggtgtaat cggaactgcg cacggtaagt tcttcggtat cggcgcgcgc
    17881 atcgatccac ggctggcgta gttttgccag cccttgctgc acgttaatgg cgatctgcgg
    17941 atcaccatac gggccggagg tgtcgtagac cggaatcgct tcgttttctt cgtactgcgg
    18001 ctgttctttg ctaccgccaa ttagcgtcgg gctaagctgg atctcacgca tcggcacgcg
    18061 cacgccgggg tgtgtgccag tgatataaat gcgttttgag ttgggaaagg cggtgccttc
    18121 cagggtgtcg ataaaatgtt gggcccgggc gcgttgttcg cggcgggtca gttttgttgc
    18181 agacatagct cattccaaaa agttaaggac gtggcttgtc agacgacgga tgaagcaaga
    18241 gacgatcgcc gcaaaggcga tgcgatagca gattaactct tgttcccttc gcaggtatta
    18301 gcctgatcag gttccgcgga tcccgaataa acggtctcag ccagttaagg cactccgaca
    18361 agaaattagc ccccacaaac ggggcgttga atgtaaatta cgcgttaaca gcgcagaact
    18421 caagcaggat gtttgacgcg ggcggcgtca agcaccagca gaatcagctt atcttccagc
    18481 acaaagcgcg cttccagcgc ctcgccgatg tcagataaaa cctgttgaaa ctcaaggtaa
    18541 ttatcatgat cgatggcggt ttccaggctg gaatcgtagt aatccataat ctgttgggta
    18601 ttggcttcga gttgcggcca aatcttcgcg gcgcgcgcga gttgcccgtt gccttccagc
    18661 ttatgaagaa tgcgctcata aatactgaaa tgtccggcag acaagtaatc gaccaggctc
    18721 tgacaaaaat catcaagggc tttttcgttt agcctcatgt acgattcttt gccaggctta
    18781 atgccaacca gattgtagta agccacgagc agatgcttac gtacatgtag ccagcgatca
    18841 accagtttgt tacttcctct gacgcgctcc gtcaggttat cgagctggtt aagcatgatt
    18901 gactccgcaa gtttgtattc aaaaactgct cagtgagaaa tgtaaaaacc atgttaaaca
    18961 tgccagtgat gcaaaggtag tgcaagagct atggatcgta taattgaaaa attagatcac
    19021 ggctggtggg tcgtcagcca tgaacaaaaa ttatggttgc cgaagggaga attgccatat
    19081 ggcgaagcgg caaatttcga tcttgtgggt cagcgcgcac tacaaatcgg cgaatggcag
    19141 ggggaacctg tttggttaat acaacagcag cggcgtcacg atatggggtc ggtacgtcag
    19201 gtcattgatc tcgatgttgg gctgtttcaa ctggccggac gtggcgtgca actggcggag
    19261 ttttaccgat cgcataaata ctgtggttac tgcgggcatg aaatgtatcc gagcaaaacc
    19321 gaatgggcga tgctgtgtag ccattgccgt gagcgttact acccgcaaat cgccccctgc
    19381 attattgttg ccatccgtcg cgatgattcg ctcctcctcg cccagcatac ccgccatcgt
    19441 aacggtgtcc atacagtact tgccggattc gtcgaagtgg gcgaaaccct cgagcaggca
    19501 gtcgcgcggg aagtgatgga agagagcgga attaaagtta aaaacttgcg ttacgtgact
    19561 tctcagccgt ggccgtttcc tcagtcttta atgaccgcgt ttatggcgga atatgacagc
    19621 ggcgacatcg tgatcgaccc gaaagaattg ctcgaggcga actggtatcg ctatgacgat
    19681 ttgccgttac tcccgccgcc cggcaccgta gcgcgccgtc tgatagaaga tactgtggcg
    19741 atgtgtcggg cagagtatga gtgatgatac actgaccgcc tgacgcacta aggaacagcc
    19801 aaaatgaccg aacttaaaaa cgatcgttat ctgcgggcgc tgctgcgcca gcccgttgat
    19861 gtcactccag tatggatgat gcgccaggcg ggtcgctatc taccggaata taaagccacg
    19921 cgcgcccagg cgggcgattt tatgtcgctg tgcaaaaacg ccgagctggc gtgcgaagtg
    19981 actttgcaac cgctgcgtcg ctacccgctg gatgcggcga tcctcttttc cgatatcctc
    20041 accgtgccgg acgcgatggg gttagggctc tattttgaag ccggagaagg tccgcgtttt
    20101 acctcgccag tcacctgcaa agccgacgtc gataaactgc caattccgga cccggaagat
    20161 gagctgggtt acgtgatgaa cgcggtgcgt accattcgtc acgaactgaa aggcgaagtg
    20221 ccgctgattg gtttttccgg cagcccgtgg acgctggcaa cctacatggt ggaaggcggc
    20281 agcagcaaag ccttcaccgt gatcaaaaaa atgatgtatg ccgatccgca ggcgctgcac
    20341 gctctgctcg ataaactggc gaaaagcgtc actttgtatc tgaatgcgca gattaaagcc
    20401 ggtgctcagg cagtgatgat tttcgacacc tggggcggcg tgcttaccgg gcgcgattat
    20461 caacagttct cgctctatta catgcataaa attgttgatg gtttactgcg tgaaaacgac
    20521 ggtcgccgcg tgccagtcac gctgtttacc aaaggcggcg gacagtggct ggaagccatg
    20581 gctgaaaccg gttgcgatgc gctgggcctc gactggacaa cggacatcgc cgatgcgcgc
    20641 cgccgtgtgg gcaataaagt cgcgttgcag ggtaatatgg atccgtcgat gctgtacgcg
    20701 ccgcctgccc gcattgaaga agaagtagcg actatacttg caggtttcgg tcacggcgaa
    20761 ggtcatgtct ttaaccttgg tcacggcatt catcaggatg tgccgccaga acatgctggc
    20821 gtgttcgtgg aggcagtgca tcgactgtct gaacagtatc accgctaaga gcctgatcca
    20881 tcaggctatt ttatttgcca ttttgaggcc gggcagtgct caaaatcctc acgtactacg
    20941 tgtacgctcc ggtttttccg cgctgtccgg cctcaaactg ccttcaacaa ttacgcctgt
    21001 tgaaccaggc tctaaggagc gattatggat ctcgcgtcat tacgcgctca acaaatcgaa
    21061 ctggcttctt ctgtgatctg cgaggatcga ctcgataaag atccaccgga tctgatcgcc
    21121 ggagccgatg tcgggtttga gcagggcgga gaagtgacgc gagcggcgat ggtgctgctg
    21181 aaatatccct cgcttgagct ggtggagtat aaagttgccc gcatcgccac caccatgcct
    21241 tacattccag gttttctttc cttccgcgaa tatcctgcgc tgctggcagc gtgggagatg
    21301 ctgtcgcaaa agccggattt agtgtttgtc gatggtcatg ggatctcgca tcctcgccgt
    21361 cttggcgtcg ccagccattt tggcttattg gtggatgtgc cgaccattgg cgtggcgaaa
    21421 aaacggctct gcggtaaatt cgaaccgctc tccagcaaac cgggcgcgct ggccccactg
    21481 atggataaag gcgagcagct ggcctgggtc tggcgcagca aagcgcgctg taacccgttg
    21541 tttatcgcta ccggccatcg ggtcagcgtg gacagcgcgc tggcgtgggt acaacgctgc
    21601 atgaaaggct atcgtctgcc ggagccaacg cgctgggcgg acgcggtggc ctcggaacgt
    21661 ccggcgttcg tgcgctatac agcaaatcag ccctaattca ggtaaactgc ggataatttc
    21721 cgtatttgag aactcaacat gttacaaaac ccaattcatc tgcgtctgga gcgcctggaa
    21781 agctggcagc atgtcacttt catggcttgc ttatgcgaac gcatgtaccc caattacgcc
    21841 atgttctgcc agcaaaccgg ttttggtgat gggcaaattt accgtcgtat tctcgatctc
    21901 atctgggaaa cgctgaccgt taaagacgca aaagtaaatt tcgacagcca actggagaaa
    21961 tttgaagaag cgattccttc agccgacgat ttcgatctgt acggcgttta tccggcaatc
    22021 gatgcctgcg tggcgttaag tgaactggtc cattcgcgtt tgagtggtga aacgctcgaa
    22081 cacgcggtgg aagtgagtaa gacctccatc acgaccgttg cgatgctgga aatgactcag
    22141 gctggtcgcg aaatgggcga tgaagagctt aaagaaaacc cagctgtaga gcaagaatgg
    22201 gacattcagt gggaaatatt ccgactttta gctgagtgcg aagaacgcga tatcgagctg
    22261 ataaaaggcc ttagggcaga cctgcgtgag tcgggtgaga gcaatattgg tataattttt
    22321 cagcaataag accagaaaac gtgatttaac gcctgatttg tcgtacctgg agtcttccct
    22381 ttcgcccccc gtctggtcta catttggggg gcgaaaaaaa gtggctatcg gtgcgtgtat
    22441 gcaggagagt gctattctgg catttccgtc gcactcgatg cttagcaagc gataaacaca
    22501 ttgtaaggat aacttatgaa caagactcaa ctgattgatg taattgcaga gaaagcagaa
    22561 ctgtccaaaa cccaggctaa agctgctctg gagtccactc tggctgcaat tactgagtct
    22621 ctgaaagaag gcgatgctgt acaactggtt ggtttcggta ccttcaaagt gaaccatcgc
    22681 gctgagcgta ctggccgcaa cccgcagacc ggtaaagaaa tcaaaattgc cgcagctaac
    22741 gtaccggcat ttgtttctgg caaggcactg aaagacgcag ttaagtaaga ttgcgtggca
    22801 gtgaacagtt ttaacgaagg ggtggtttca ccccttttgt ctttctggcg tcgatcattg
    22861 atgctggcag gtgcgctgtt tctcactgcc tgtagccaca actcttcact tcctcccttt
    22921 accgccagtg gatttgctga agaccagggc gcggtacgca tctggcgaaa agacagcggc
    22981 gataatgtgc atctgcttgc cgtgtttagc ccgtggcgca gtggcgatac cacgacgcga
    23041 gagtatcgct ggcagggcga taacctcacg ctcatcaata tcaatgttta cagcaaaccg
    23101 ccggtgaaca ttcgcgcgcg ttttgacgat cgcggcgatc tgagctttat gcaacgtgaa
    23161 tccgatgggg aaaagcagca gctttctaac gaccaaatcg atttataccg ttatcgtgct
    23221 gatcagatcc gccagattag cgatgcctta cgtcagggga gagtcgtgct gcgccagggg
    23281 cgctggcatg cgatggaaca gaccgtgacc acctgcgaag ggcaaaccat taaacctgat
    23341 ttagattcgc aggcgatagc gcatatcgag cgccgccaga gccgctcttc tgttgatgtc
    23401 agcgtggcat ggctggaagc gcccgaaggt tcgcaattac tgttagtggc aaactctgat
    23461 ttctgtcgct ggcaacccaa cgagaaaacg ttctgattta ccagtggccc atacccatat
    23521 gaccgccacc accgcagccg ccgtagccca tgcccattcc ggctccgcgc ggaatacccg
    23581 cttcagccat cgcgatatct cgtttcaccc gtaactcatc taacgactga cgcaaattct
    23641 ccatctcttt ggcgaccgcg ttaattttgc tgctatccgg tgggttcgcg gctaacaggg
    23701 cattgtattc ataacgcttc gtcaccagtt gctgttgcag tgcgctgctt tgagcgtaaa
    23761 agtcattatg gattttctgc cacgctgtct gttgttcgct ggtcaaaggc gcggcatttt
    23821 gctgccacat accgtgtccg ccgtgagcaa atgcagatgt cgatcccatc gccattgctg
    23881 aaagcgccat cattaccagg gcaattttcg tgttccgttt catggttaat cctccagtgg
    23941 ttgtcttact tcgggtattg catcttccgt gccaacgatg aaacgctgat atgacgggta
    24001 atctggcatg ataaacgagt aaaaatgact cgcctgctgc gggtagcgag tcatttttac
    24061 tcattgaaac ttaagccttt gtgttacagc gcagggtaag cgctgataaa agatggcatg
    24121 atttctgctg tcagaaaggg atgagcaggc aaagaagaag atgcgtttta tgcaacgttc
    24181 taaagactcc ttagctaaat ggttaagcgc gatcctcccc gtggtcattg ttgggctggt
    24241 agggttgttt gcggtgacgg tgattcgcga ttatgggcgc gagactgccg ccgccagaca
    24301 aacgctgctg gaaaaaggca gtgtacttat tcgcgctctt gaatccggct cgcgcgtcgg
    24361 catggggatg cgcatgcatc atgcgcagca gcaggcctta ctggaagaaa tggccgggca
    24421 gcctggagta cgttggtttg cggtcacgga tgaacaagga acaatcgtga tgcatagcaa
    24481 ctccggcatg gtgggaaaac agctttattc ccctcaggaa atgcagcagt tacatccggg
    24541 agatgaagaa gcgtggcggc ggatcgatag cgcagacggc gagcctgttc tggaaattta
    24601 tcgccagttt caaccgatgt ttgctgctgg aatgtaccgg atgcgccata tgcagcagta
    24661 tgccgcgaca ccacaagcaa ttttcatcgc tttcgacgcc agtaatattg tgagtgccga
    24721 agatcgtgag cagagaaaca ccctgattat tctcttcgcc ctggcgacgg tcttgctggc
    24781 aagcgtattg tcattcttct ggtatcgccg ctatctgcgc tcgcgccagc ttctacaaga
    24841 tgaaatgaag cgcaaagaga agctggtggc gctggggcat cttgcggcag gcgttgccca
    24901 cgaaatccgt aacccacttt cctcgattaa aggactggcg aaatactttg ccgagcgcgc
    24961 gcctgcaggg ggagaagcgc atcaactggc gcaggtgatg gcgaaagagg ccgaccgttt
    25021 aaaccgcgtg gtaagcgagt tgctggaact ggttaagcca acgcatctgg ctttgcaggc
    25081 ggtggatctc aacacgctga ttaaccactc attacagctg gtaagccagg atgcaaacag
    25141 ccgggagatc cagttacgct ttaccgccaa cgacacatta ccggaaattc aggccgatcc
    25201 ggacaggctg actcaggtcc tgttgaatct ctatctcaat gctattcagg cgattggtca
    25261 gcatggcgtg attagcgtga cggccagcga aagcggcgcg ggcgtgaaaa tcagcgttac
    25321 cgacagcggt aagggaattg cggcagatca gcttgaagcc atcttcactc cgtacttcac
    25381 caccaaagcc gaaggcaccg gattggggct ggcggtcgtg cataatattg ttgaacaaca
    25441 cggtggtaca attcaggtcg caagccagga gggaaaaggc tcaacgttca ccctctggct
    25501 tccggtcaat attacgcgta aggacccaca aggatgacgc acgataatat cgatattctg
    25561 gtggtggatg atgacattag ccactgcact attttgcagg ctttactgcg cggctggggc
    25621 tataacgtcg cgctggcgaa cagcgggcga caggcgctgg agcaggtgcg ggaacaggtt
    25681 tttgatcttg tgctttgcga tgtgcgaatg gcggagatgg acggcatcgc cacgctgaaa
    25741 gagatcaaag cgttaaaccc ggcaattccg gtgctgatta tgactgcgta ctccagcgtc
    25801 gagacggcgg tagaggcact gaaaactggg gcgcaggatt atctcatcaa gccgctggat
    25861 ttcgataacc tacaggcgac gctggaaaag gcgctcgcgc atacgcacag tattgatgct
    25921 gaaacacctg cggtgactgc cagccagttc ggtatggtcg gtaaaagccc ggcgatgcaa
    25981 cacctgctca gtgaaatcgc cctcgtcgcg ccatcggaag ccacggtgct gatccacggc
    26041 gattccggca ccggtaaaga gctggtcgcc agggcgattc acgccagtag cgcacgtagc
    26101 gaaaaaccgc tggtaacgct caactgtgcg gcactcaacg aatccttgct ggaatctgaa
    26161 ttgttcggtc acgaaaaagg ggcgtttact ggagccgata aacgccggga agggcgcttt
    26221 gttgaggcgg acggcggcac gctgtttctc gatgaaattg gcgatatctc gccgatgatg
    26281 caggtgcgtc tgctgcgtgc gattcaggag cgcgaagttc agcgtgtcgg tagcaaccag
    26341 actatctcgg ttgatgtccg gctgattgcg gcgacccatc gcgatcttgc cgcagaggtg
    26401 aatgccgggc gttttcgcca ggatctctac tatcgcctga atgtggtggc gattgaagta
    26461 ccatcgctgc ggcaacggcg ggaagatatc cctctgctgg ctggccattt tcttcagcgc
    26521 tttgccgagc gtaatcgcaa ggcggtaaaa ggctttacgc cacaggcgat ggatttgctg
    26581 atccactacg actggccggg aaatattcgt gaactggaaa acgcggtgga acgggcggtg
    26641 gtgctgctga ccggggaata tatttccgaa cgcgagctgc cgctggcgat tgccagtacg
    26701 ccgatcccgc tggggcaaag tcaggatatt cagccgctgg tggaagtgga aaaagaggtg
    26761 attctggcgg cgctggagaa aacgggcggc aacaaaaccg aagccgcccg tcagttaggg
    26821 atcacgcgca aaacgctatt ggcaaaactg tcgcgttagt tctgctcgcg ttcgatggcg
    26881 cgccaaccga tatctttccg gcagaagcaa tcatcccagt ggatatcggt cattaaggcg
    26941 taggcgcgtt tctgcgcttc tgccacggta tgacccagcg cggtgacgca cagtacgcgc
    27001 ccgccgctgg ttaccacctg ctcgtcatcc gccagttttg tgcccgcgtg gaacactttg
    27061 ccgtcagcca cttcttccag cggcaggccg tggatcacat cgccggtgcg gtaatcgccc
    27121 ggatatccac ccgcagccat caccacgctg agagaagcac gttcatccca ctctgacgtt
    27181 ttctcgtcca gtttgccttc acaggccgcc aggcagagct caaccagatc ggacttcatg
    27241 cgcagcataa tcggctgggt ttccggatcg ccaaagcggc agttaaattc aataaccttc
    27301 ggattgccct gtttgtcgat catcaggccc gcatagagaa aaccggtgta ggtgttgcct
    27361 tccgccgcca tgcctttcac ggttggccag atgatacgtt ccatggtgcg ctgatgaact
    27421 tcatcggtta ctaccggcgc gggggagtaa gcgcccatcc cgccggtgtt tggtccgtta
    27481 tctttatcgc ctacgcgttt gtgatcctgg ctggtagcca tcggcagcac atgctcgccg
    27541 tccaccatca cgataaagct cgcttcttcg ccatcgagga actcttcgat aacgatgcga
    27601 tgacccgcgt cgccaaaagc gttgcccgcc agcatatcgt gaacagccgc ttccgcttct
    27661 tccagcgtca tcgccacgat aacgcctttc ccggcagcca gaccgtccgc tttaatgacg
    27721 attggcgcgc ctttctcacg cagatacacc agcgcaggtt ctacctcggt gaagttctgg
    27781 tattccgccg tagggatctt atggcgggcc aggaaatctt tggtaaacgc ttttgagcct
    27841 tccagttggg ccgcacctgc ggttgggccg aagattttca gcccggcggc gcggaaggta
    27901 tcgaccacgc ctttcaccag cggcgcttcc gggccgacga tggtcagatc aatcttttcg
    27961 ttttgtgcga aatccaacag cgccgggata tcggtcacgc cgatagcaac gttttgcagc
    28021 gcgggttcca gcgcagtgcc tgcattaccc ggagcagcaa aaacagtctc aaccagcggc
    28081 gactgggccg ctttccaggc cagcgcgtgc tcgcgcccgc cgttaccaat cactaatact
    28141 ttcatctatt gctccattaa tggcggaagt ggcgcatgtc ggtgaagagc atcgcaatac
    28201 cgtgctcgtc ggcggcggca atcacttcgt catcacggat ggaaccgcca ggctggatta
    28261 cgcaggtcac gcccgcagcg gcggcggcat caataccgtc gcggaacggg aagaacgcgt
    28321 cagaagccat cgaggaacct ttcacttcca ggccttcatc ggcagcttta ataccggcga
    28381 ttttcgcgga gtacacgcgg ctcatctggc ccgcgccaat accgatggtc atattgtttt
    28441 tggcatagac gatagcattg gatttcacga atttcgccac tttccagcag aacagcgcat
    28501 cacgcagttc ctgttcggtc ggctgacgtt gggtgacgac gcgcagttct tccgcaccga
    28561 ccatccccag gtcacgatcc tgaaccagca gaccgccgtt cacgcgtttg aaatcaagac
    28621 ccggaacacg ctcgccccac tgaccgcagg tcagaacgcg tacgttctgc ttggcggcgg
    28681 tgattttcag ggcttcttcg ctggcggaag gcgcaataat cacttcgaca aactgacgag
    28741 aaatgatggc ctgtgcggtt tccgcatcca gctcgcggtt aaaggcaatg atgccgccga
    28801 atgcggaggt tgggtcggtt ttgtacgcgc gatcgtaagc atcaagaatg gaattgccga
    28861 tagctacacc gcaagggttg gcgtgcttca caatcacaca tgccggctcg gcgaactctt
    28921 tcacgcactc cagcgccgca tcggtatcgg cgatgttgtt ataagagagg gctttaccct
    28981 gaacctgggt tgcggtagca acggaggctt ctttcacatt ctcttctata tagaaggcag
    29041 cctgctggtg gctgttctcg ccgtaacgca tatcctgctt cttaatgaag ttcaggttca
    29101 gcgtgcgtgg gaagcgaccg gcggcttctt tgctttcacc gtggtacgcc ggaaccatgc
    29161 tgccgaagta gttggcaatc atgctgtcgt aggcggcagt gtgttcgaag gctttgatgg
    29221 cgaggtcgaa acgggttgca agcgtcagcg atccttcgtt gtcatccatc tctttaataa
    29281 tggcgtcgta gtcgctgctc ttcaccacga ttgcgacatc tttatggttc ttagcggcgg
    29341 agcgcaccat ggtcgggccg ccgatatcga tgttctcaac cgcatcttcc agcgagcagc
    29401 cttcacgagc aacagtctgg gcgaacggat acaggttaac aaccaccata tcgataggct
    29461 ggatctgatg ttcttccata atggcatcgt cctggccgcg acggcccaga atgccaccat
    29521 gtactttcgg atgcagggtc ttcacgcgtc catccatcat ctccgggaaa ccggtgtaat
    29581 cggaaacttc ggttaccggc agaccttttt ctgctaacag acgggcagtg ccccctgtag
    29641 acagcagctc cacaccgcgt gcggaaagtg cctgggcgaa ttcgacgata ccggctttgt
    29701 cagaaacact gagcagagcg cggcggactg gacgacgttg ttgcatggta aatcccctgg
    29761 gtttgactat tacagagagc gttagctgaa tttttcgcga aaaactcagc taacgcccct
    29821 aacggggcat ccttattttt cgcccgcatt gtaacgaaaa cgtttgcgca acgctcgcga
    29881 atttttctct ttcaatggtg atcacaattt tgacagtggt taccgtggga aaaatacaga
    29941 aattacattg atgattgtgg ataactctgt gcgtaaaaag gtataaagcg gggttttgct
    30001 ggggaatgca gcagtcagtc atttttctgc aatttttcta ttgcggcctg cggagaactc
    30061 cctataatgc gccttcatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc
    30121 ggttgaaga
//
LOCUS       DAZXUK010000021        29063 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZXUK010000021 DAZXUK010000000
VERSION     DAZXUK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29063
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.021"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..143)
                     /gene="hybE"
                     /locus_tag="ACH3L8_000780"
     CDS             complement(<1..143)
                     /gene="hybE"
                     /locus_tag="ACH3L8_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[NiFe]-hydrogenase assembly chaperone HybE"
                     /protein_id="HFT4084017.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTL"
     gene            complement(136..630)
                     /locus_tag="ACH3L8_000781"
     CDS             complement(136..630)
                     /locus_tag="ACH3L8_000781"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFT4084018.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(630..2333)
                     /gene="hybC"
                     /locus_tag="ACH3L8_000782"
     CDS             complement(630..2333)
                     /gene="hybC"
                     /locus_tag="ACH3L8_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFT4084019.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(2330..3508)
                     /gene="hybB"
                     /locus_tag="ACH3L8_000783"
     CDS             complement(2330..3508)
                     /gene="hybB"
                     /locus_tag="ACH3L8_000783"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFT4084020.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(3498..4484)
                     /gene="hybA"
                     /locus_tag="ACH3L8_000784"
     CDS             complement(3498..4484)
                     /gene="hybA"
                     /locus_tag="ACH3L8_000784"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFT4084021.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(4487..5605)
                     /gene="hybO"
                     /locus_tag="ACH3L8_000785"
     CDS             complement(4487..5605)
                     /gene="hybO"
                     /locus_tag="ACH3L8_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFT4084022.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(5794..6081)
                     /gene="yghW"
                     /locus_tag="ACH3L8_000786"
     CDS             complement(5794..6081)
                     /gene="yghW"
                     /locus_tag="ACH3L8_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFT4084023.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(6200..7087)
                     /gene="yghX"
                     /locus_tag="ACH3L8_000787"
     CDS             complement(6200..7087)
                     /gene="yghX"
                     /locus_tag="ACH3L8_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFT4084024.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            7244..8284
                     /gene="gpr"
                     /locus_tag="ACH3L8_000788"
     CDS             7244..8284
                     /gene="gpr"
                     /locus_tag="ACH3L8_000788"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFT4084025.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(8324..8818)
                     /locus_tag="ACH3L8_000789"
     CDS             complement(8324..8818)
                     /locus_tag="ACH3L8_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFT4084026.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9009..9893
                     /gene="yghA"
                     /locus_tag="ACH3L8_000790"
     CDS             9009..9893
                     /gene="yghA"
                     /locus_tag="ACH3L8_000790"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFT4084027.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPSIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(10165..10590)
                     /gene="exbD"
                     /locus_tag="ACH3L8_000791"
     CDS             complement(10165..10590)
                     /gene="exbD"
                     /locus_tag="ACH3L8_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFT4084028.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(10597..11331)
                     /gene="exbB"
                     /locus_tag="ACH3L8_000792"
     CDS             complement(10597..11331)
                     /gene="exbB"
                     /locus_tag="ACH3L8_000792"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFT4084029.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            11583..12770
                     /gene="metC"
                     /locus_tag="ACH3L8_000793"
     CDS             11583..12770
                     /gene="metC"
                     /locus_tag="ACH3L8_000793"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFT4084030.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            12910..13569
                     /gene="yghB"
                     /locus_tag="ACH3L8_000794"
     CDS             12910..13569
                     /gene="yghB"
                     /locus_tag="ACH3L8_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFT4084031.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(13609..14508)
                     /gene="yqhC"
                     /locus_tag="ACH3L8_000795"
     CDS             complement(13609..14508)
                     /gene="yqhC"
                     /locus_tag="ACH3L8_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFT4084032.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            14702..15865
                     /gene="yqhD"
                     /locus_tag="ACH3L8_000796"
     CDS             14702..15865
                     /gene="yqhD"
                     /locus_tag="ACH3L8_000796"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFT4084033.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            15970..16797
                     /gene="dkgA"
                     /locus_tag="ACH3L8_000797"
     CDS             15970..16797
                     /gene="dkgA"
                     /locus_tag="ACH3L8_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFT4084034.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            16997..17923
                     /locus_tag="ACH3L8_000798"
     CDS             16997..17923
                     /locus_tag="ACH3L8_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFT4084035.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            17974..18231
                     /gene="yqhH"
                     /locus_tag="ACH3L8_000799"
     CDS             17974..18231
                     /gene="yqhH"
                     /locus_tag="ACH3L8_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFT4084036.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(18274..20493)
                     /locus_tag="ACH3L8_000800"
     CDS             complement(18274..20493)
                     /locus_tag="ACH3L8_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFT4084037.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(20604..22016)
                     /gene="ftsP"
                     /locus_tag="ACH3L8_000801"
     CDS             complement(20604..22016)
                     /gene="ftsP"
                     /locus_tag="ACH3L8_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFT4084038.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(22091..22828)
                     /gene="plsC"
                     /locus_tag="ACH3L8_000802"
                     /gene_synonym="parF"
     CDS             complement(22091..22828)
                     /gene="plsC"
                     /locus_tag="ACH3L8_000802"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFT4084039.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(23062..25320)
                     /gene="parC"
                     /locus_tag="ACH3L8_000803"
     CDS             complement(23062..25320)
                     /gene="parC"
                     /locus_tag="ACH3L8_000803"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFT4084040.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(25866..26348)
                     /locus_tag="ACH3L8_000804"
     CDS             complement(25866..26348)
                     /locus_tag="ACH3L8_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFT4084041.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSELIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(26401..26793)
                     /gene="ygiW"
                     /locus_tag="ACH3L8_000805"
     CDS             complement(26401..26793)
                     /gene="ygiW"
                     /locus_tag="ACH3L8_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFT4084042.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            26945..27604
                     /gene="qseB"
                     /locus_tag="ACH3L8_000806"
     CDS             26945..27604
                     /gene="qseB"
                     /locus_tag="ACH3L8_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFT4084043.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            27601..28875
                     /gene="qseC"
                     /locus_tag="ACH3L8_000807"
     CDS             27601..28875
                     /gene="qseC"
                     /locus_tag="ACH3L8_000807"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFT4084044.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(28921..>29063)
                     /locus_tag="ACH3L8_000808"
     CDS             complement(28921..>29063)
                     /locus_tag="ACH3L8_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2645 domain-containing protein"
                     /protein_id="HFT4084045.1"
                     /translation="LTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLFWGRFIF
                     CY"
BASE COUNT         7198 a         7732 c         7331 g         6802 t
ORIGIN      
        1 aacagcgtaa aatcagaaac atacaccggc attgaaggat gcagaaaaga gagatcgtgc
       61 atcgaacgcc gggcaatttc ttcaaacgct gcctgtactt gcgccttcgg ggaggtctgg
      121 aaacctgcta tctcttcagt catgaatcgc ctcccgtggg atggcttcca cgcctgattc
      181 gcgcagcgca gccagaacct gctcaagcgc aggttcaatc attgcttcaa ccgtcggcgt
      241 taagccgatg tgtggctcca gcgattccgg gatcacgccg accagggtca gctttttcgg
      301 aaactcaccg gtgaagcgca gggccgacaa tacgtcggcc aggccaagct gatgcggaga
      361 gattttgttg gtaaacaacg ccggaacttc ttcatcccgc aggatcatca tcgttcccgg
      421 agtgttcttt ttcgacacaa tggcatccgc aataatcaga tgatcgcgat ttgccatgtc
      481 gccaagcagc tccattcccg ccgtgccgcc atcgaggatc tcaacataat ccggcagaat
      541 gtatcgttgc tctaacgctt cgacaatccg cacgccgatg gcttcatcgg tcagcaaaat
      601 attgccgacc cctaagacta aaatacgcat tacagaacct tcactgaaac cacttcgttg
      661 ccgtcggcat ccactacgtg taccgcacag gccatgcacg ggtcgaagga gtgaatggta
      721 cgcaccactt ccagcggttt attcggatcg gcaaccggtg tacccaccag cgactgctcg
      781 taaggaccga cgtcatcatt gaagttacgc ggaccagagt tccaggttga tggaacaacc
      841 gcctggtagt tgctgatgat accgtcttta ataaccatcc agtgagagag cataccgcgc
      901 ggcgcttcga ggaagccaac acctttgaac tcacccgttg ccggaatgtt cggcttcaca
      961 aaggtggtgt gatcgccttt gccgatattg gtgatcagtg cactgtattg gttttgcagg
     1021 atatcctgca attcacagca gtgaacggta cgaccaataa tacggcccag cgtggagtgc
     1081 agctgtgcca cttccagcgt gttgccagtc agtttctgat aaatcgcaac gatttcattc
     1141 agtttgttct gggtagattc gcgacctgcc gccagtttca ccagcatatt agccagcgga
     1201 cccacttcta ccgttttgcc gtagaaagtc ggtgatttca cccaggaata tttcccgtcg
     1261 tcagaccaac catcataagc cggaatggtg gtaccttccc acggcgcctg tggcgcttcg
     1321 tctttatacc aggagtgctt cgcgctttcc tgaatgcctt tgatcaggta ttcatcggaa
     1381 tgagaagtga tcggacgata cgaggacaga tccgcattct caatgtagcc tcccgggaac
     1441 aggaagctgc cgtttttgct gtcggtcggg aattccggca cgctcaggta gttcaccgca
     1501 cctttaccgc gcgtcagcca ttccgggtag aacgcggcga taaccgcggt atcaacctta
     1561 taaacctgct caacaaagtc gctcagtttg tcgatgaaag acttgatgta catcaggcgc
     1621 tcaaggttca acacgcccaa cccgtcgagg ttgattgggt tcgcgacacc acccacagcc
     1681 aggttctgaa tgtgcggcgt tttaccgccc agcagcgcca cgacgcggtt agcgtcacgc
     1741 tggcactcca gcgcctgcag gtagtgcgct accgcaatca ggttcacttc cggcggcagt
     1801 ttcatcgccg ggtgacccca gtagccattg gcgaaaatac ccaactgacc gctggcaacc
     1861 agatctttga tcttgttctg aactttagtg aactcttccg gactgttcag atgccaggtc
     1921 gaaacgcctt tcagcatttc ggaggctttg gttgggtcag cttgcagtgc agaagtgatg
     1981 tccacccagt ccagcgccga aagctgatag aaatgaacga tatggtcatg cgtggtgtgc
     2041 gcagccagaa tgatgttacg gatgtattgc gcgttaaccg gaacgtcgat attcagcgca
     2101 ctttctgccg cacgaacgga agacagcgcg tgagtggtag tacatacgcc acagatacgt
     2161 tgcacaatca tccatgcatc gcgcggatcg cggtttttca cgatctcttc catgccgcgc
     2221 cacatggtac cggaagccca tgctttcgaa acgacgccat tttcgatttc gcaatcgatg
     2281 cgtaaatgcc cctcaatacg ggttaccgga tcaatagtaa ttctctggct catgctttgc
     2341 tcgcctcatg acgattatga tcgttttgtt ttaaaggagg aagtatcggc agtagacgaa
     2401 tgagtacgat gtaagcgcaa atctcaatag ccacaaaacc aatagaaatc aacagttctt
     2461 cccaggtcgg gaagtaggcg taaccgccgc ccgggttgaa tgccaccaac gaataggtca
     2521 gacgccaggt tgcacaacct aacagtgcgc tcagtgctga caggaacagc atacgggaat
     2581 cattacgcag cttcgccaca cgcagaacga ccagcgggaa gagcatcagc aggacttcaa
     2641 tccagaacat cacggagtag aagtcaccgg caaacgctaa cgacagcttg tcgcgataga
     2701 taagctcgcc aaagcgcagc ccgatgaaaa tcgccagcaa cacactgatg gtgttggtca
     2761 gcttgacgaa caggctcttt tcatccggac cgttgccacg cagacccgcc tgcaccagtg
     2821 aaccttcaaa gatgacaatc gagaagccca tgatgaacgc cgtcagcagc gagaacagcg
     2881 gcaacatttc atagctctgc cagagcggat gcaccttgta gcccgccgag atcatcagcg
     2941 accccattga agactggtgc atggtcggca gcagcgcacc gagcgcgatg atgaagaaca
     3001 tcaccttgtt cagacgttgt agcgacacct tccagcccag acgttcaaac agtgccggag
     3061 caaactccag agccatcacg ccgatgtaga tggtcataca gaccgccgtc tcgaacagta
     3121 ccgagttcac gttgaagtga cccggaatgt agaagtacgg caggttccag tagcgaccca
     3181 cgtcgatagt gatcgacaag ccacccagtg agtaaccaaa cagactcgcc aacagcgccg
     3241 gacgcaccag cggatggtat tgcccacggt taaagacgta taccgcccac gccagcgccc
     3301 agccgccaca ggcaaagccg gtgccaatca gcaggtcaaa cgcgatccac acgccccacg
     3361 ggaagccgcc gttcaggtca gagacagagc ccagaccgaa caccagacgc ttcacaatca
     3421 ggagcataca gatgacgatt aacggtccaa aaatcatgac cggtttactg atgattttgc
     3481 cgcccagcgg ttgtggatca tgactcatga tcgtctcctc cgtcgtgatg gtcgtttttg
     3541 gtgttgcgac gaaccagcac ggttaagccc gccagcacag ccagtggtag catcatgcct
     3601 ttatacaggg tgtgttgaat attttcggaa cgcgcaccgg tagaaagatc gtccagtttc
     3661 ggcaggtcga gattttcata aggcacaccc gtcagtacca gaacctgagt accgccgccc
     3721 tctttctcgc cgtacagatg cggataatat ttcggcaccg tatgcaggta agtgtcgcca
     3781 gatttcagcg tctgacgcgg atagtggtat tcgctgccag gcttcagcgc cagacgtttt
     3841 ttcgcctccg ccatcagctc ttcacgcgta ccgaaaatca ccgcgcccgc cgggcacact
     3901 tctacgcagc caggcagacc gcctttatcg agacgttcca cacctttctg gttgcacagc
     3961 tcgcacttat gcagcgcacc aaacgggttg ttgtagtcgt acttcggcac gttgtacgga
     4021 caggcgacca tgcagtaacg gcaaccggtg cacacgtctt tgtcgtaatg gacaatgccg
     4081 gttttcggat cttttttcag tgcagagacc gggcacacag agacacagtt cggatcgacg
     4141 cagtgcatac actgtttctt aatgtacgcg tagccgttct cctcctggtc tttgttgacc
     4201 cctgtgccgc tggtccacac ctgaatgatg ttattggtat acggcgacag tttgtcgttg
     4261 ttcgaccagg tctgttcccc ttgcgggtta cgttcaggga agttgatatc ctgacacttg
     4321 gtgacgcaag cctggcagcc tacgcacaag gtcgagtcgt acaacatccc cagcgatccc
     4381 ggaattggcg ggcggttttc agcagccgca tgactgacag acggcagcgc gcccgtcagc
     4441 aatgccccgc aggaggctgc tttaataaaa ttacgtctgt tcacggttat tctccccgtg
     4501 agtcagcgtt atctttcttt tgctgacgac ccagttcacg caccgccatc acgctgacac
     4561 cggcaaccag cccaaccaca ccgccgagca aaccaatagc gcctgcagag acgttgccgc
     4621 cctctttagc gttaacatcc ggtttctgtg aacgcggagt ctgattttcg acgttggcaa
     4681 gctgatggat gcctttatgg aagccgatac ctttttcgtt acagccatag cacgggtgac
     4741 caatcgccac cggccacaca ccgccaacat cgcagaattg cagcgttgag cagttgccgt
     4801 aagtttctgg ccctttacag ccgaggtggt acaagcacca gccttcgcgg tggccttcat
     4861 caccgaactc tttggcaaaa cgaccagcat cgaagtgcgg gcgacgttcg cagtgttcgt
     4921 gaatcagacg gccataggcg aaggtcggac ggtttttgtc atccagtttc ggcggtttgc
     4981 cgtaagtgat gatgtgcgca acggtcgcga ggaagttgtg cgggttcggc gggcagcccg
     5041 gaatattgat aacggttttg cctggcagaa cttcttgcaa gctgactgcg ccagttgggt
     5101 taactccagc tgcggcaaca ccgccccacg cggagcagga accaatagcg ataatggctg
     5161 ctgagccttc cgccgctttg cggatgtgat ccacaatcgg ctcaccggca accatgcaat
     5221 aaataccgtt atcttttaat gggatggaac catccaccac taacacatac tgccctttgt
     5281 acttctcgag agcgttatgt ttgttctctt cgacctgatg accgaaggcg gcggaaagca
     5341 cttcgtgata ttccagagag atagtctcca gcacgaggtt ttctaccgtt ggatgcgttg
     5401 cacgaagcag agattccgta caaccggtgc actcctgcgc gccaatccag ataactggcg
     5461 gacgctgcgg gttagtaacc gattcggcca tctctgcagc ggctttgcta cttaacccca
     5521 tggtggcggc taatgctgca caaagcttca tgaaatcacg acggttaatg ccgtgagaat
     5581 ggatgagggt gttatctcca gtcatttata gttattccgt tgcgagaacc tggcatatat
     5641 tttgcctcaa tcgcaaaatc aataatgcga tcgatgcgcc atttaccaca catttattat
     5701 ggttatcgat atcatgatac tgcggcgggg cgctaaaacg aagggaagaa gcattagtgt
     5761 aattaattaa agcaagataa tacgtatgtt tgatcaattt tcgaggcgat aaccggccat
     5821 aaaaaagcgc aacgtagaac aggaattatt ctcacgaaag aaatccatta ccatctcttt
     5881 atccagtcca tagcggcgcg tcagaatacc cgcttcccag gcgctaagct ggcgatctcc
     5941 ggtcttttcg tacatcctgt gtgagtagcc taatacaaaa ccgcgtttat aatcggcaca
     6001 gaattttgtc acattaaccg cactgtcggc atgcgttgct tttaatcccg ccattaagcc
     6061 tttaccaaaa tggttattca tcgttcacct cattcattat atatcgccat atataacgat
     6121 attttaggca aagaacgact tttgcgcacg ctattttttc atacttgttt aagacgtaat
     6181 cccgtaagag ataaacctat caggagagat atttatcgaa ccatttcagt gtcctttgcc
     6241 aggcaagatc ggcggcagat ttgtcataac ggggcgtgga atcattatgg aatccgtgat
     6301 taacccccgg atagatataa gcctcataaa ccttattatt gtctttcaac gccgcctcgt
     6361 aaggaggcca gccctcgttg attcgggtgt ccagttccgc gaagtggagt agtaaaggcg
     6421 cttcaatctt cgccacatcg gcagtgggtg cctgacgacc ataaaacggc accgcgcagg
     6481 ccagttccgg atacgcgaca gccgccgcgt tcgatacgcc accgccatag caaaatccgg
     6541 taatacccac tttacctgtc gcttgcggat agcgttgcat aaactcaatt gcggcaaaga
     6601 aatcattcat cagtttggtt ggatcaacct gctgttgcag ctcacgacct ttatcatcat
     6661 ttcccggata acctccaacg gaacttaatc cgtcaggtgc cagggcgata taccccgcct
     6721 tcgccactcg ccgtgccaca tcttcgatat acggattcag tccacgattc tcatgcacca
     6781 cccccacggc tggcgttttg ccgctcattt ttgcgggttt caccagataa ccccgtacct
     6841 cgccgtgacc atttggcgaa ggatacgtga tgtactcagc aacaatctcc gggtcggtaa
     6901 actctacctg agtcgccagc gcataatttg gcttgagcaa atcaaacaac gctaatgccg
     6961 tcatcccgcc caccgcatac ttcgccgcaa ggttgaggaa ctcacgtttc gaaattttcc
     7021 cgtgaacgta atagtcgtag taatccaaca actcttgtgg gaaatctttg gcggttaaac
     7081 gcggcatcgt tgcattcctc aactggtgtt tttttaagca aagcataagc gcgtgttttt
     7141 gcccagtttt tcgtcactct gtgagccaga ctacgggata cgcgctggcg aatcgctaaa
     7201 ctagaaacat tgtttcgaaa ttgaacggtg gaaaggagag gtcatggtct ggttagcgaa
     7261 tcccgaacgt tacgggcaga tgcagtaccg ctattgcgga aaaagtggtt tacgcctgcc
     7321 cgcattatcg ctcggtttat ggcacaattt cggtcacgtt aacgcgctgg aatcacagcg
     7381 tgcaatcctg cgtaaagcgt ttgatttagg cattacgcac tttgatttag ccaacaatta
     7441 cgggccgcct ccaggaagcg cagaagagaa ctttggtcgc ctgctgcgcg aggattttgc
     7501 tgcttatcgc gatgaactga ttatctctac caaggctggc tacgatatgt ggcccggccc
     7561 gtacggctct ggcggttcac gtaaatacct gctcgccagc ctcgaccaaa gcctgaagcg
     7621 tatggggctt gagtatgtcg atatctttta ctctcatcgc gtcgatgaaa atacgccgat
     7681 ggaagaaacc gcctctgcgc tggctcatgc ggtacaaagc ggtaaggcgc tgtatgtcgg
     7741 gatctcctct tactcgccag agcggacgca aaaaatggtc gagttgctgc acgagtggaa
     7801 aattccgctt ttaattcatc aaccttcgta caatttactg aaccgctggg tggataaaag
     7861 cggcctgctg gataccctgc aaaataacgg cgtgggctgt attgccttta ctcctctggc
     7921 tcagggattg ctgaccggaa aatatctcaa cggcattccg caagattcac ggatgcatcg
     7981 tgaagggaat aaagttcgtg gtctgacgcc gaaaatgctt accgaagcca acctcaacag
     8041 cctgcgctta ttgaatgaaa tggcacagca gcgtggacaa tcaatggcgc aaatggcgtt
     8101 aagctggttg ctgaaagatg atcgcgtgac gtcggtattg attggtgcca gccgcgcgga
     8161 gcaactagag gagaacgtgc aggcgctgaa taatctgaca tttagcaccg aggagctggc
     8221 gcagattgat cagcatatcg ccgatggcga gctgaatctg tggcaggcgt cgtccgataa
     8281 atgacctgtt aataacgggc gcgcgaaccg cgcccgaatc agatcagtga ttatgacgag
     8341 tcagtcggtc aagatagccc atcacaaatg cagaaagcac aaacgtcaga tggataatga
     8401 cgtaccacat cagtttgtta tccgggacat ttttcgcatc cataaagacg cgcagtaagt
     8461 gaatggaaga aattgccaca atcgacgctg ctactttgtt tttcagcgac gttgcgtcca
     8521 ttttccccag ccacttcagc ttctctttgt tctcggagat atccagctgt gagacgaaat
     8581 tctcataacc ggaaaacatc accatcacca gcaaaccgcc aaccagcgtc atatccacca
     8641 gcgacagcaa cacgaggatc aaatctgatt ccgccatcga gaagatattc ggcagtacgt
     8701 gaataatctc ctggaagaac ttcagcgcca gggcaactaa cgccagcgaa aggccaaagt
     8761 acacgggggc aagcagccag cgagaagcat acattgcatt ttcaagaaaa cgttccatac
     8821 attcctgtcg tgtagttaaa acgggcaaca gtatatcgca attgagtaaa cagcacgcaa
     8881 cagcaaatca acatatacgt taaacctttg tcgcataatt ctgaggttta gggatcttcc
     8941 tggttccctt atcaacgcat cgagagcgtc tacccttaaa ggacgttgaa cctgaaggga
     9001 gaaaaacgat gtctcattta aaagacccga ccacgcagta ttacactggt gaatatccca
     9061 aacagaaaca accgacgcca agcatccagg cgaagatgac accgataccg gattgcggcg
     9121 agaaaaccta tgttggtagc ggtcgcctga aagatcgtaa agcactggtg acagggggcg
     9181 attccggaat aggtcgcgct gccgccatcg cttacgcgcg tgaaggggct gacgtggcga
     9241 tcagttatct tcccgtggaa gaagaagacg ctcaggatgt gaaaaagatc attgaagaat
     9301 gcggacgcaa agccgttctg ctgccaggcg atttaagcga tgagaaattc gcccgttcgc
     9361 tggttcacga agcgcacaag gcgttaggcg ggctggatat tatggcgctg gtcgccggga
     9421 aacaggttgc cattccggat attgcagacc tcaccagcga acagtttcaa aagacctttg
     9481 ccattaacgt tttcgcgctg ttctggctaa cccaggaagc gatccccctg ctaccgaaag
     9541 gtgcaagtgt catcaccact tcgtcaatcc aggcatacca gccaagtccg catttactgg
     9601 actatgcggc tacgaaggcg gcgattctga actacagccg tggcctggca aaacaggtcg
     9661 cggagaaagg tattcgggtg aatattgtcg cgccaggccc tatctggacg gcactgcaaa
     9721 tttccggcgg acaaacgcag gataagatcc cgcagtttgg tcagaaaacg ccgatgaagc
     9781 gtgcggggca accggcggaa ctggcccctg tatatgttta tctggcaagt caggagtcga
     9841 gctacgtcac cgcagaagtg cacggcgtgt gcggcggcga gcatttaggt taaaaaaatg
     9901 cccggttgtg aaaagcaacc gggcatcatt gtgaattcac tcttacctga tgtggcgaaa
     9961 acgccttatc cgcctacaaa cgcatgaaga ttcaacatgt tgcaatagat tttgtaggcc
    10021 tgataagcgt agcgcatcag gcattgagca cctaatgccg gatgcgtcgt gaacgcctta
    10081 tccggcctac aaacgcacga agattcaaca tgttgcaata gattttgtag gcctgatgag
    10141 cgtagcgcat caggcaattc tactttactt cgctttggcg gtttcttcgc cgaccagacc
    10201 tatcttcagg taacccgcct gatgcagcgt atccattacc ttcatcaacg tctcgtaatc
    10261 gacggttttg tccgctcgga agaagatggt ggtgtctttc ttgccttcgg ttaacgcatt
    10321 caacgccgta atcattgttt catcggtgac cggatcgtta ccgataaaca tcgagttgtc
    10381 tgccttcacc gacagataaa ccggtttttc cgggcgcggc tgcggcgtgc tggtagaagc
    10441 aggcaagttc accttcacat ctaccgtcgc taacggtgcc gccaccataa agataatcag
    10501 cagaaccaac atcacgtcga taaacggcgt cacgttgata tcatgcattt cgccgttatc
    10561 gtcgaggttt tcgttaagat gcattgccat cggacattat cctgcgcgta atttttgtgc
    10621 gacacgaacc ggatgcgcag cggcgctggc ttccagatcc aggtcacggc tttgcagcaa
    10681 caatacctgc gctgcaacat cacccagcat cgctttaaag ccgccaatct ggcgtgcaaa
    10741 tacgttatag ataacgaccg caggaatcgc tgccacgagg ccgattgccg ttgctaacag
    10801 agcttctgcg atacccggcg caacgactgc caggttagtg gtctgcgttt gcgcgatacc
    10861 aataaagctg ttcatgatgc cccagaccgt accaaacaga ccaacgaacg gagaaatcgc
    10921 gccgatggtt gccagatagc cgttaccgcg gcccatttga cgacccactg cggcgacccg
    10981 acgctccaga cggaagctgg tacgttcttt aataccttcg ttatcgtcgc tgccttctga
    11041 cagttccagc tcattctgcg cttcattgag caaatgcagg cttaagcttt tgctaccaaa
    11101 atcagcggcg atatcgttgg cctggtttaa ggaacgtgct tcagccagca gttgctgctc
    11161 gcgcttaagg cgacgcttct gattgaagaa ctctacgctc ttactgaaga agattgccca
    11221 ggtgactaca gaggccaaaa taagcccaat catcacgcac ttaacgacaa tatcggcgta
    11281 ctgatacata ccccagacgg aaaggtccgt ctgcattaaa ttattaccca ctgtgtatct
    11341 ccaggacgca agtcacaaaa tctgcgcata ataatatcaa aacgacgtcg aattgatagt
    11401 cgttctcatt actatttgca tactgccgta cctttgcttt cttttccttg cgtttacgca
    11461 gtaaaaaagt caccagcacg ccatttgcga aaattttctg ctttatgcca attcttcagg
    11521 atgcgcccgc gaatattcat gctagtttag acatccagac gtataaaaac aggaatcccg
    11581 acatggcgga caaaaagctt gatactcaac tggtgaatgc aggacgcagc aaaaaataca
    11641 ctcttggcgc ggtaaatagc gtgattcagc gcgcttcttc gctggtattt gagagtatgg
    11701 aagccaaaaa gcacgcgacg cgcaatcgcg ccaatggcga gttgttctat ggacggcgcg
    11761 gaacgttaac ccatttttcc ttacaacaag cgatgtgtga actggaaggt ggcgcaggct
    11821 gcgcgctatt tccctgcggg gcggcagcgg ttgctaattc gattcttgct tttgtcgaac
    11881 agggcgatca tgtgttgatg accaacaccg cctatgaacc gagtcaggat ttctgtagca
    11941 aaatcctcag caaactgggc gtaacgacat cgtggtttga tccgctgatt ggtgccgata
    12001 tcgttaagca tctgcagcca aacactaaaa tcgtgtttct ggaatcgccg ggttccatca
    12061 ctatggaagt ccacgacgtt ccggcgattg ttgccgccgt gcgcagtgtg gtgccggatg
    12121 ccatcattat gatcgacaac acctgggcag ccggtgtgct gtttaaggcg ctggattttg
    12181 gcatcgatgt ttctattcag gccgccacca aatatctggt tgggcattca gatgcgatga
    12241 ttggcactgc cgtgtgcaat gcccgttgct gggagcagct acgggaaaat gcctatctga
    12301 tgggccagat ggtcgatgcc gataccgcct atataaccag ccgtggcctg cgcacattag
    12361 gtgtgcgttt gcgtcaacat catgaaagca gtctgaaagt ggccgaatgg ctggcagaac
    12421 atccgcaagt tgcgcgagtt aaccaccctg ccctgcctgg cagtaaagga cacgaattct
    12481 ggaaacgaga ctttacaggc agcagcgggc tattttcctt tgtgcttaag aaaaaactca
    12541 atgatgaaga gctggcgaac tatctggata acttcagttt attcagcatg gcctactcgt
    12601 ggggcgggta tgaatcgttg atcctggcaa atcaaccaga acatatcgcc gccattcgcc
    12661 cacaaggcga gatcgatttt agcgggacct tgattcgcct gcatattggt ctggaagatg
    12721 tcgacgatct gattgccgat ctggacgccg gttttgcacg gattgtataa cattgccact
    12781 tttggacaat tttgcagaca ttttattgtg aaaagtctta aattgttgcg tccgggatca
    12841 aggcgtcccg gacgattcag gagtacaata ggcagataaa ggcttaaacg ctgttccaca
    12901 ggaaagtcca tggctgttat tcaagatatc atcgctgcgc tctggcaaca cgactttgcc
    12961 gcgctggcgg atcctcatat tgttagcgtt gtttactttg tcatgtttgc cacgctgttt
    13021 ttagaaaacg gcctgctgcc cgcctcattt ttgccaggcg acagcttgtt gatactggca
    13081 ggcgcattga ttgcccaagg ggttatggat tttctgccta cgattgcgat tctgaccgcc
    13141 gcagcaagtc tgggctgctg gctaagttat attcagggga gctggttagg gaataccaaa
    13201 acggtgaaag gctggctggc acagcttcct gctaaatatc accagcgcgc cacctgcatg
    13261 tttgaccgcc acggtctgct ggcgctgctg gctggacgtt ttcttgcatt tgtccgtacg
    13321 ctgctgccaa ccatggcggg aatttccggt ctgccaaacc gccgcttcca gtttttcaac
    13381 tggttaagtg gattgctgtg ggtcagcgtg gtaaccagtt ttggctatgc cttaagtatg
    13441 attccgttcg ttaaacgcca tgaagatcag gtaatgacgt tcctgatgat cctgccaatt
    13501 gccttgttaa ccgctggctt gttaggcacg ctgtttgtgg tgattaaaaa aaaatactgt
    13561 aacgcctgac gattttcccc gttcccggct gctgtaccgg gaacgtattt aattcccctg
    13621 catcgcccgc attcttgccg catcttcccc cggcgtcaca ccgaagtaac gtttaaactc
    13681 acggctaaat tgcgatgcgc tttcatagcc gacgcgcatc gctgctgcgc tggccttcat
    13741 gccgtcatgg atgatcatca tccgcgcctt atgcagacgg taattcttca aatactgcaa
    13801 cggcgaggtg ctggtgacag acttaaaatt atggtggaac gccgatacgc tcatgttggc
    13861 ttctgccgcc agttgctcga cgctcaggtt ttcggtgtat ttattctcaa ttcgtttcag
    13921 cacgcggcta atcagactga agtgagtctg gcgactgacc agcgccagta acgcgccgcc
    13981 gcaaggtccg gtcagcacgt agtacagaat ttcgcggatg atctgtttgc cgagaatacg
    14041 cgcatccagt ggtcgctcca tcacgtcgag taaccgctcc gccgcgcata aaatctcttc
    14101 tgataacgtg gcggagttaa tcccgctggc tgccatcgac ggctggaaat gctcatcttc
    14161 gccaatgtcc atcaacagtt cctgtaactg caaaatatcg acattgagac gcaaccctgc
    14221 cagcggcacc tctgacgtgg cataggtttc gcactcaaac ggcaacggca ccgtcagcag
    14281 caggtattca ttggcatcat aacgaaacac gcgttcattg atataaccga ttttatgccc
    14341 ggaaaagaga attatgatgc caggctcgta catcaccggt gtacgtgcga aaggcgtctc
    14401 gccatacaac aaacgcacat cgggcaacag ttctgacaaa ctattttctt tatttttcag
    14461 tttattaact ttatccgcca gcaagcgaca aatctcttca cgtttcatat cgcgtaattt
    14521 cttaggaata atgcggcaat ttgattgtgc gcaattttgt agcatttctc cagcactctg
    14581 gagaaatagg caagacattg gcagaaatga gcattgagag ccagggcgct ggcgatcaca
    14641 atgaaaaaca tcaggcagat cgttctctgc cctcatattg gcccagcaaa gggagcaagt
    14701 aatgaacaac tttaatctgc acaccccaac ccgcattctg tttggtaaag gcgcaatcgc
    14761 tggtttacgc gaacaaattc ctcacgatgc tcgcgtattg attacctacg gtggcggcag
    14821 cgtgaaaaaa accggcgttc tcgatcaagt tctggatgcc ctgaaaggca tggacgtgct
    14881 ggaatttggc ggtattgagc caaacccggc ttatgaaacg ctgatgaacg ccgtgaaact
    14941 ggttcgcgaa cagaaagtga ctttcctgct ggcggttggc ggcggttctg tactggacgg
    15001 caccaaattt atcgccgcag cggctaacta tccggaaaat atcgatccgt ggcacattct
    15061 gcaaacgggc ggtaaagaga ttaaaagcgc catcccgatg ggctgtgtgc tgacgctgcc
    15121 agcaaccggt tcagaatcca acgcaggcgc ggtgatctcc cgtaaaacca caggcgacaa
    15181 gcaggcgttc cattctgcac atgttcagcc ggtatttgcc gtgctcgatc cggtttatac
    15241 ctacaccctg ccgccgcgtc aggtggctaa cggcgtagtg gacgcctttg tacacaccgt
    15301 ggaacagtat gttaccaaac cggttgatgc caaaattcag gaccgtttcg cagaaggcat
    15361 tttgctgacg ctaatcgaag atggtccgaa agccctgaaa gagccagaaa actacgatgt
    15421 gcgcgccaac gtcatgtggg cggcgactca ggcgctgaac ggtttgattg gcactggcgt
    15481 accgcaggac tgggcaacgc atatgctggg ccacgaactg actgcgatgc acggtctgga
    15541 tcacgcgcaa acactggcta tcgtcctgcc tgcactgtgg aatgaaaaac gcgagaccaa
    15601 gcgcgctaag ctgctgcaat atgctgaacg cgtctggaac atcactgaag gttccgacga
    15661 tgagcgtatt gacgccgcga ttgccgcaac ccgcaatttc tttgagcaat taggcgtgcc
    15721 gacccacctc tccgactacg gtctggacgg cagctccatc ccggctttgc tgaaaaaact
    15781 ggaagagcac ggcatgaccc aactgggcga aaatcatgac attacgttgg atgtcagccg
    15841 ccgtatatac gaagccgccc gctaagcttt ttacgcctca aactttcgtt ttcgggcatt
    15901 tcgtccagac ttaagttcac aacacctcac cggagcctgc tccggtgagt tcatataaag
    15961 gaggaacgta tggctaatcc aaccgttatt aagctacagg atggcaatgt catgccccag
    16021 ctgggactgg gcgtctggca agcaagtaat gaggaagtaa tcaccgccat tcaaaaagcg
    16081 ttagaagtgg gttatcgctc gattgatacc gccgcggcct acaagaacga agaaggtgtc
    16141 ggcaaagccc tgaaaaatgc ctcagtcaac agagaagaac tgttcatcac cactaagctg
    16201 tggaacgacg accacaagcg cccccgcgaa gccctgctcg acagcctgaa aaaactccag
    16261 cttgattata tcgacctcta cttaatgcac tggcccgttc ccgctatcga ccattatgtc
    16321 gaagcatgga aaggcatgat cgaattgcaa aaagagggat taatcaaaag catcggcgtg
    16381 tgcaacttcc agatccatca cctgcaacgc ctgattgatg aaactggcgt gacgcctgtg
    16441 ataaaccaga tcgaacttca tccgctgatg caacaacgcc agctacacgc ttggaacgcg
    16501 acacacaaaa tccagaccga atcctggagc ccattagcgc aaggagggaa aggcgttttc
    16561 gatcagaaag tcattcgcga tctggcagat aaatacggca aaaccccggc gcagattgtt
    16621 atccgctggc atctggatag cggcctggtg gtgatcccga aatcggtcac accttcacgt
    16681 attgccgaaa actttgatgt ctgggatttc cgtctcgaca aagacgaact cggcaaaatt
    16741 gcaaaactcg atcagggcaa gcgtctcggt cccgatcctg accagttcgg cggctaacgt
    16801 gcaaattctc ccggtggcgg taatgttccg ctaccggact tttcagaaat catttattcc
    16861 cctcgcgtcc cgcccgttgt tactcttcct tgttcaggaa tgccaaatat aaggacatca
    16921 tcatgcagag ccggaagctc ttaaaagaac aactcatcta tatccgggat aaacgcaacg
    16981 gagaggtgaa aaacagatga aaataatact tctgttttta gcagccctgg caagttttac
    17041 cgtacacgcg cagcctcctt cacagaccgt agaacaaaca gtccggcaga tttatcagaa
    17101 ctataaatca gatgccagta cgccttattt cggtgaaacc ggagagcggg cgataacttc
    17161 agcgcgtatt caacaggcgc ttaccctgaa cgacaatctt acactaccgg gcaatattgg
    17221 ctggctggat tatgatccgg tttgtgattg tcaggatttt ggcgatctgg tgttagaaag
    17281 cgtagcgata acccaaactg acgtcgatca tgccgatgcc gttgtgcgct ttcgtatctt
    17341 taaagatgat aaagaaaaga ccacacagac actgaaaatg gtggcggaaa atggtcgttg
    17401 ggtcattgat gatattgtca gcaatcatgg cagcgtctta caagcagtta atagcgagaa
    17461 tgaaaaaacg ctggccgctt tagcttcgtt gcagaaagaa cagccagaat cctttgttgc
    17521 tgaactcttt gaacatattg ctgattatag ctggccgtgg acgtgggtgg tttccgactc
    17581 ttaccgccag gcggttaatg ccttctataa aaccaccttc aagacggcca ataatcccga
    17641 tgaagatatg caaatagaac ggcaatttat ttacgacaat ccgatctgtt ttggcgaaga
    17701 gtcgctattt tcacgcgttg atgaaattcg agtcctggag aaaaccgccg attccgcccg
    17761 cattcatgtt cgttttacgc tgaccaatgg caacaatgaa gagcaagagc tggttttaca
    17821 gcggcgcgaa ggtaagtggg aaatcgctga ttttatccgc ccgaacagcg gcagcctact
    17881 taagcagatt gaggcaaaaa ccgccgccag attaaagcaa tgagctgaat taaataacaa
    17941 ttagccggaa caataaataa aagggaacac tatatgaaaa cgattttcac cgtgggagct
    18001 gttgttctgg caacctgctt gctcagtggc tgcgtcaatg agcaaaaggt caatcaactg
    18061 gcgagcaatg tacaaacatt aaatgccaaa atcgcccggc ttgagcagga tatgaaagca
    18121 ctacgcccac aaatctatgc tgccaaatcc gaagctaaca gagccaatac gcgtcttgat
    18181 gcacaggact attttgattg cctgcgctgc ttgcgtatgt acgcagaatg ataaaaaaat
    18241 ccccggcagc atgtcagttg ccggggattt tttttaacgt ccaaccgccg ctttagggcg
    18301 tttcttcgca ccagcattca ccggacgaga ttgcgtagac gacgcttttt ttgccgtagc
    18361 aggcgtctga cgctgagtcg ccatcggcgt atgtttagtc aacgccggac gggtattgcg
    18421 gttctggcga cgagcttcac gcatctcttc aatggttggc gcaggcacta agcaatcgcg
    18481 acggctgcca atcagatgct ttttgcccat cgcttccagc gcctggcgga ttaacggcca
    18541 gtttgccgga tcgtggtaac gcaacaacgc tttatgcaaa cgacgctgtt tgtcgccctt
    18601 cggtacgaag acgccttcgc tcttataacc aatcttcgcc agcggttttt tcccggtgta
    18661 atacatggtg gttgagttcg ccagcggcga tgggtagaag ttctgtacct ggtcgagacg
    18721 gaaacgatgc tttttcagcc acagcgccaa attcaccata tcttcatcac gcgtaccggg
    18781 gtgcgcagag atgaaatacg ggatcagata ctgctcttta cctgcctgtt tcgagtaggt
    18841 atcgaacagc tctttaaagc ggtcatagct gcccatgccc ggcttcatca tcttcgacaa
    18901 cggcccttct tcggtatgtt ccggagcaat cttcagataa ccgccgacgt gatgggtcgc
    18961 caactctttg atatagcgcg gatcttccac ggcgatgtca taacgtacgc ctgaggcgat
    19021 aaggattttt ttaatgcctt tcagatcacg cgcacggcga tagaggttga tcgtcggttc
    19081 gtggttagtg tccatgtgcg gacaaatatc cggataaacg cacgacaaac ggcgacaagt
    19141 ttgttcagcg cgtggcgatt tgcagcgcaa catatacatg ttggcagttg gcccaccgag
    19201 atcggaaatc acgcccgtaa aacctggaac ggtgtcgcgg atcgcttcga tctcattaat
    19261 gatcgaatct tccgaacggc tctgaataat gcgcccttcg tgctcggtaa tagaacagaa
    19321 agaacagccg ccaaagcagc cgcgcataat gttgaccgaa aaacggatca tttcgtaagc
    19381 cggaatacgg gcattgccat aggccggatg tggcacgcgc ttgtacggca gcgcaaaaac
    19441 gctgtccatc tcttcggtag aaagcgggat agcaggcggg ttgatccaca catagcggtc
    19501 accgtgtttt tgcatcaatg cgcgggcgca gcctgggttg gtttcgtggt gcagaatgcg
    19561 cgaagcatgg gcgtacagca ctttatcgcc cttcactttc tcgaaagaag gcagcaatac
    19621 gtaggttttt tcccacggtt tctggcgcgg tggctgcacg attacagctt tcgcttcctg
    19681 ctttttcggc gcaacaggtt tgttatccgc acacggcaaa tcttcgccat acggatgcgg
    19741 gattgggtcg attttcccta gggtatcaag acgggtggaa tccacaccgc tccagccagg
    19801 cagcgcctct tttacgataa ttgcggtatt acgcacatcg cggatttcac taatcggctc
    19861 gcccatcgcc agacgatgcg ctacttccac cagcggacgt tcaccgttac caaacatcag
    19921 catgtcggct ttcgaatcta ccagaacgga acggcgcacg gtatcggacc agtaatcata
    19981 atgcgcggta cggcgcaggc tagcctcaat accgccgagg atcaccggca catctttcca
    20041 cgcctcttta caacgctggg tataaaccag cgtggcgcga tccggtcgct tacccgcgac
    20101 attatctggc gtgtaggcat catcatgacg taaacggcga tcggcggtat agcggttgat
    20161 catcgaatcc atgttgccag cagtaacacc aaaaaacaga ttcggtttac ccagacgcat
    20221 aaagtcgtct ttgctgctcc agtccggctg ggcgatgatc ccgacgcgaa agccctgcgc
    20281 ttccagcata cgaccgcaaa tcgccatccc gaagcttggg tgatcgacat acgcgtcgcc
    20341 agtaaccaaa atgatgtcgc agctatccca gccaagttga tccatctctt cacgagacat
    20401 cggcaaaaac ggtgccggtc caaaacaggc ggcccagtac tgcggccagg agaacaggtc
    20461 gcgatccggt tggatcaggg agatagagct cattttgctt ccagaaatga taaaaaaata
    20521 atcaaaggcc ggggattata agccggaacg aaagagaaat cgaaaggtat tccatactcg
    20581 ccctcctcgg gcgagtatga agattacggt accggattga ccaacagttg cccaatcgag
    20641 ccacggtccg ccatttccag cgtctgactg ttgaagtaga acgggaagtg cgcccaggaa
    20701 ggctgaccga aataaacaag cagctccact tgtccgtcaa cccaaacggt atctttccag
    20761 cctctgtctt ccgggaacgg catcgcgcca ttcacgttac ggatctggaa cattacgcct
    20821 tcaatatgga acgcttgcgg ctcgtccgcg cgtaccgtcc agcgttccca cgttccttgc
    20881 tgcgcggtga catcaatacg gttgacgtcc cacagctgtc cattaatacc cgggtcatca
    20941 cccagactga tatcgcgact gcgaattggc gaaccagcca tgatttcagt tggcagcaag
    21001 cgcatcggaa gactgtctgt gaccagcggc agaaggccgg ttgggcgcag cgttagcacc
    21061 aaggtagaaa ccagaatact ggatggctca aagaagccgc gaatacgatc aacaatgctc
    21121 gccgcttcac cacaggtgat cgacacttca tcgccgttgc tcatatccac cagaatctcg
    21181 cggcgctcgc ccggtgccag cgaaagttgc ttcaccgata caggagcagg caggaatccc
    21241 tgatcgccag aaatcacatg taacgggcga ccatcgctca tctgtagttg atagcgacga
    21301 gagttcgacg cgttcagcag tcgcaagcgc acccagccac gcgagacttc aacgtacggg
    21361 ctttgtacac cgttaaccag cagcgtatca ccaacaaagc cgccgcttcc cggttcgttg
    21421 tattctggcg taccaaagtt atccagccgt ttatcctgga taatgaccgg aaaatcatcc
    21481 acaccataat ggttggggat aggcagcgac ttgctgactt catcttccac cagccacatt
    21541 ccggcaaggc cgttatagac ctgctgagcc gtgcggttgg gagtattggc gtgataccac
    21601 agagtagctg cgttctggcg aataggcagt acgggtgccc agtcagcgtt tggcgacatc
    21661 atccgtgccg gaccgcccat cagcgggcct ggtacctgta gcccggccac cgtcattgag
    21721 acattttctg tcaggcggtt gctgtaaata agcttaacat cgtcgccctt ccagacgcgg
    21781 atagtcggcc ccaggtaacg accattgatt ccccagaccg acgcgcgtgt ccctggcgta
    21841 aatgaccagt gcgcacgttg tacagtcata aacagcggtt gcccacggcg agattcaagt
    21901 agcggcggaa cgggtagcgg ttgctgttgc ccggctgcgc tggccttcag gggaacagcg
    21961 cctgcacaaa gtgcaatccc cgatgcctga atgaactgac gccgactgag tgacatattt
    22021 gctccatgta aaactggcta aagtaatacc gtgcaggaaa gtgttccccg caaaaataca
    22081 gattgccgac ttaaactttt ccggcggctt cgcgttctgc gacttcttta tcgagctcgg
    22141 cgattttttg ttccattatc gaacgacaat gggcagccag ctcacgaacc tgatctttgc
    22201 catactgact gacgtcaatt ggcggcagca tttcgacaat caccagaccg ttgtgcagtc
    22261 gattaagatt aatcttattc gaagttgtag agacgcacac gggaataatc gggacgcccg
    22321 ccgcaattgc cgcgtgaaat gctccagtct tgaacggtag caggccgcga ccacggctgc
    22381 gggttccttc cgggaacatc cagatggaaa tacggcgttt tttgaaatga ttcacgactt
    22441 ccgcaatggt gccgtgagct ttagtgcgat tgtttctgtc gatcaataag ttgccggtta
    22501 accagtacaa ctgcccgaag aaggggatcc acagcaagct ctttttacct accgtcaccg
    22561 tcggcggttg cacaatgttc gatgctgtca ccatgtcata gttgttctgg tggttagcga
    22621 tatagatagc attgccgtag ctttcagcat ctgcaggttt gcggcactca actttcaggc
    22681 cgaacagtgg cgcaagacgg ccaaacatat gcccaaaggt ggccacatgt ttcgggttac
    22741 gcgggctgaa aaggcagtaa atggagccga atacacagac taagatgctg taaatcacgg
    22801 taataataag acgaaagata tatagcataa caccctctga aggtctgaga gactggcatt
    22861 gtacgtcacc tgaaatcagg ttagggcatt gttaaaagcc gctctctgga aaagcgtatt
    22921 gttaatcgca acgcatgaat aaacaacggt ttcaagggaa tttttatcgt ttccgggggt
    22981 tgaatacgct gccggatgac gacttaacgt ttcatccggc gttccttgct agcgggagga
    23041 aacagcgccc tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga
    23101 gtcgatctca acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg
    23161 ttcgccagtg actttctgta gctcttccgg acgcagttta attttgcgtt tcccaacatg
    23221 aatggtcagc gtgctttgcg gcggcagaac gtacagttgc gccagaccat cctcgccacg
    23281 cgcggcttct gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg
    23341 cagatcgctt accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc
    23401 ggaagcatct tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa
    23461 agccttaccc gcacggttac gcgctaccag atcgttaaag gtgcagacga aaccgtaacc
    23521 cgcatcggaa gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt
    23581 cgccccaggc ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg
    23641 cagcgtaatc gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg
    23701 gttactctta cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg
    23761 cgcgtcgata tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt
    23821 gacaggttca gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg
    23881 caacggcgaa cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag
    23941 caggttattc attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc
    24001 cagctcactc tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt
    24061 cagttcgagg atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg
    24121 tttcggttca tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac
    24181 cagcaaacct tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg
    24241 gcggcgcacg gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt
    24301 caccgccgga cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag
    24361 atcggtggta gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg
    24421 cacaatcacc agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat
    24481 cggcagcttt ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac
    24541 ctgatgcggt aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat
    24601 acgcactgaa ccacgtccgt tctcgtagat tttacgaatc tcggcgcgcg aagtgataat
    24661 ttccgcttca gtcggataat ccggcccctg cacgatatcc agcagctgat cgagcgtagt
    24721 tttcggctgg tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg
    24781 aatatcggtc gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag
    24841 acgggcaggt agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc
    24901 cgtcccctgc cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt
    24961 gtaacgcatt gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc
    25021 aaccagcgga taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc
    25081 gctatcgccg tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt
    25141 aaatttggcg ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac
    25201 aggtttcaga ccatcaccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta
    25261 gtttaagtag gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat
    25321 taattctgat tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact
    25381 acattatctg ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc
    25441 cgacggcgat aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg
    25501 gcatagaggg cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc
    25561 gggcgattcg agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc
    25621 gactgtaagt atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc
    25681 tgccataagt gcggcttgct catggatatc cccgagacgg cttacggcgc accggataat
    25741 aaatttcgcg tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc
    25801 ctgtgacatt gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt
    25861 ccccatcacg ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg
    25921 gcaagattgg tgtagtgaaa cagaatcggt gctttacgca ttttctcgcc gcttgcaggc
    25981 agccagtggc gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg
    26041 cgggccacgg cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg
    26101 ggaatcagtt cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg
    26161 gcaggggcgt cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc
    26221 tctttacgcc acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg
    26281 tgcggcaaca tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg
    26341 tttgtcattt tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa
    26401 ttacggattt actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc
    26461 gacttcaccc tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt
    26521 gtgatcgata tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga
    26581 gatgcgttca acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc
    26641 gctttctaca gtcgttacgc tgccgttcgg cccctgaaat cctccggcct gactttgcgt
    26701 taccgatggg ccagaaaaac cgccctgctc tgctgccatc accggtgcac tgcacagggc
    26761 cattactgcg attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt
    26821 ccgagcattt aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttaatg
    26881 ctgtacagag cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga
    26941 aaaaatgcga attttactga tagaagatga catgctgatt ggtgacggca tcaaaactgg
    27001 ccttagtaaa atgggtttta gtgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc
    27061 gctttatagc gcaccttatg atgcggtgat cctggattta accttaccag gcatggatgg
    27121 tcgcgatatt ttgcgcgaat ggcgagagaa aggtcagcgt gagccggtac tgaacctgac
    27181 cgcgcgtgat gcgttagcgg aacgtgtaga agggctgcgt ctgggagctg acgattatct
    27241 gtgtaaacct tttgcgttga tagaagtcgc cgccaggctg gaggctctga tgcgccgaac
    27301 caacggccag gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg
    27361 tatcgccacg ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga
    27421 attactgatg cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta
    27481 tacctgggac gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg
    27541 caaactcggt agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa
    27601 atgaaattta cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg
    27661 gcctcggtga cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc
    27721 gatgaattgt tcgacaccca actgatgctg tttgccaaac ggttaagtac gctcgatctc
    27781 aacgaaatca acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat
    27841 gttgatgacg atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaat
    27901 gatggcgata acggagaaga tattccctat agctatcaac gggaaggttt tgctgacggg
    27961 caactggtcg gtgaagacga tccttggcgt tttgtctgga tgacctcacc tgatggcaaa
    28021 tatcgcatcg ttgttggcca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt
    28081 gccgggcaat tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta
    28141 ctactgggtc gtgaactcgc gccgctgaac aaactggcgc tggcactacg tatgcgtgac
    28201 cctgactcgg aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg gccactggtt
    28261 gagtcgctaa atcaactgtt cgcccgcaca catgcgatga tggttcgaga acgacgcttt
    28321 acctccgatg cagctcacga actacgtagc ccgttaacag cgctgaaagt gcaaaccgaa
    28381 gttgcgcagc tctctgacga tgatccgcag gcgcggaaaa aagcactgct ccaattacat
    28441 tccgggatcg atcgcgctac tcgtctggtt gatcaactgc tcacgctatc gcggctggac
    28501 tcactggata accttcagga cgtcgcggag atcccgcttg aagatctcct acaatcgtcg
    28561 gtgatggata tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat
    28621 gcccacggca tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg
    28681 ctggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga acgcttctat
    28741 cgcccacccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt ccagcgaatc
    28801 gccaagctgc atgacatgaa tgttgagttt gggaatgcgg aacaaggtgg atttgaggcg
    28861 aaggtaagct ggtaacttct ctgtctatga gagccgttaa aacgactctc atagatttta
    28921 ctaatagcaa aatataaacc gtccccaaaa aagccaccaa ccacaaacca gacaaataac
    28981 gagcgagaat acatatagcc aaaactcgaa catttcccgc ataaagagtt ttcttaagat
    29041 aagaataata agtggcgtaa gaa
//
LOCUS       DAZXUK010000022        28221 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZXUK010000022 DAZXUK010000000
VERSION     DAZXUK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28221
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.022"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..621
                     /gene="nfeR"
                     /locus_tag="ACH3L8_000809"
                     /gene_synonym="yqjI"
     CDS             <1..621
                     /gene="nfeR"
                     /locus_tag="ACH3L8_000809"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFT4084046.1"
                     /translation="SHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCGG
                     RHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYPT
                     LDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQN
                     PQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(672..2192)
                     /gene="aer"
                     /locus_tag="ACH3L8_000810"
                     /pseudo
     CDS             complement(672..2192)
                     /gene="aer"
                     /locus_tag="ACH3L8_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            2610..3989
                     /gene="ygjG"
                     /locus_tag="ACH3L8_000811"
     CDS             2610..3989
                     /gene="ygjG"
                     /locus_tag="ACH3L8_000811"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFT4084047.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4031..4362)
                     /locus_tag="ACH3L8_000812"
                     /pseudo
     CDS             complement(4031..4362)
                     /locus_tag="ACH3L8_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            4580..5563
                     /gene="ebgR"
                     /locus_tag="ACH3L8_000813"
     CDS             4580..5563
                     /gene="ebgR"
                     /locus_tag="ACH3L8_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFT4084048.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            5746..8832
                     /gene="ebgA"
                     /locus_tag="ACH3L8_000814"
     CDS             5746..8832
                     /gene="ebgA"
                     /locus_tag="ACH3L8_000814"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFT4084049.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            8829..9278
                     /locus_tag="ACH3L8_000815"
     CDS             8829..9278
                     /locus_tag="ACH3L8_000815"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFT4084050.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            9341..10774
                     /locus_tag="ACH3L8_000816"
     CDS             9341..10774
                     /locus_tag="ACH3L8_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFT4084051.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            10908..11979
                     /gene="ygjJ"
                     /locus_tag="ACH3L8_000817"
                     /pseudo
     CDS             10908..11979
                     /gene="ygjJ"
                     /locus_tag="ACH3L8_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            11996..14347
                     /gene="ygjK"
                     /locus_tag="ACH3L8_000818"
     CDS             11996..14347
                     /gene="ygjK"
                     /locus_tag="ACH3L8_000818"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFT4084052.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            14573..16591
                     /gene="fadH"
                     /locus_tag="ACH3L8_000819"
     CDS             14573..16591
                     /gene="fadH"
                     /locus_tag="ACH3L8_000819"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFT4084053.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(16636..17052)
                     /gene="higA"
                     /locus_tag="ACH3L8_000820"
     CDS             complement(16636..17052)
                     /gene="higA"
                     /locus_tag="ACH3L8_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFT4084054.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17049..17363)
                     /gene="higB"
                     /locus_tag="ACH3L8_000821"
     CDS             complement(17049..17363)
                     /gene="higB"
                     /locus_tag="ACH3L8_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFT4084055.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(17648..18784)
                     /gene="rlmG"
                     /locus_tag="ACH3L8_000822"
     CDS             complement(17648..18784)
                     /gene="rlmG"
                     /locus_tag="ACH3L8_000822"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFT4084056.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18869..19372
                     /locus_tag="ACH3L8_000823"
     CDS             18869..19372
                     /locus_tag="ACH3L8_000823"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFT4084057.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            19449..20141
                     /locus_tag="ACH3L8_000824"
     CDS             19449..20141
                     /locus_tag="ACH3L8_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFT4084058.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            20220..21218
                     /locus_tag="ACH3L8_000825"
     CDS             20220..21218
                     /locus_tag="ACH3L8_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFT4084059.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            21489..22454
                     /gene="alx"
                     /locus_tag="ACH3L8_000826"
     CDS             21489..22454
                     /gene="alx"
                     /locus_tag="ACH3L8_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFT4084060.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            23050..24294
                     /gene="sstT"
                     /locus_tag="ACH3L8_000827"
     CDS             23050..24294
                     /gene="sstT"
                     /locus_tag="ACH3L8_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFT4084061.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(24299..24850)
                     /locus_tag="ACH3L8_000828"
     CDS             complement(24299..24850)
                     /locus_tag="ACH3L8_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFT4084062.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(24933..26420)
                     /gene="uxaA"
                     /locus_tag="ACH3L8_000829"
     CDS             complement(24933..26420)
                     /gene="uxaA"
                     /locus_tag="ACH3L8_000829"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFT4084063.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(26435..27847)
                     /gene="uxaC"
                     /locus_tag="ACH3L8_000830"
     CDS             complement(26435..27847)
                     /gene="uxaC"
                     /locus_tag="ACH3L8_000830"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFT4084064.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
BASE COUNT         6647 a         7452 c         7353 g         6769 t
ORIGIN      
        1 agccatcatc acgaagggtg ttgtaaacat gaaggccagc cacgccatga gggctgctgc
       61 aaaggtgaga agtcagaaca cgagcactgc ggacacggtc accagcatga acacggtcaa
      121 tgttgcggtg gtcgccacgg tcgcggcggc ggtcgtcggc aacgtttctt tggtcacggt
      181 gaattgcgtc tggtgattct ggatattctc tcgcgcgatg acagccacgg ttacgaattg
      241 attaaagcga ttgagaatct aacccagggg aattacaccc caagcccggg cgtcatctac
      301 ccgacgctgg attttctgca ggagcagtcg ctgattacca tccgcgaaga ggaaggtggt
      361 aagaagcaga ttgcgctgac cgaacaaggc gcgcagtggc tggaagaaaa ccgcgaacaa
      421 gtggagatga ttgaagaacg catcaaagcg cgttgcgttg gcgcggcgct gcgccagaac
      481 ccgcaaatga agcgggcgct ggataatttt aaagcggtgc tggatttacg cgtcaaccag
      541 agcgatatca gtgatgcaca aataaaaaag atcattgcgg tgatcgaccg cgccgctttt
      601 gatattacgc aactggatta atcgccgcat ccgccagtgg cgcggtgcaa ttgccggatc
      661 cggcataccg attaatgcag caccgtcacc gcgtcttcca gtcggctggc gcggtgtttc
      721 accatcgccg acacctgcgc actctcttcc accagctcgg catttttctg ggtgatcagg
      781 ttaagctcat ccactgcacg ggtcaggctg gaaagcccat cggcctgttc cagcgttgaa
      841 tggctaatct gggcgatcaa ctgggtgacg tttttcacct gtgccacaat atcttccatc
      901 gtccgtccgg cggcgtgtac ctgctgcgaa ccggattgca ccttatcagc actggcatca
      961 atcagcttgc gaatatcgtt ggcagcatta gcgctgcggc tggctaaatg acgcacttcc
     1021 cctgccacca ctgcaaaacc tttgccctgt tcgccggcac gcgccgcttc caccgccgca
     1081 ttcagggcca gaatattggt ctgaaacgca atatcgttaa tcagcgaagt aatggtgcca
     1141 atgcgctagg tactgtcggc gatatcgtcc attgtcttga tcaccgtggt catcgcctcc
     1201 ccaccctgca ctgccgcatt actggcggtg atagaaagtt tatcggcagc cgacgccgtg
     1261 gcagagttct gtttcaccga cgccgccatt tggttcatgg tcgccaccgt ttgctgaacg
     1321 ttatcaactg tctgctgggt atgttcgttc agttcatcgg tgcctttcgc cagcgtctca
     1381 ctgccatttc tgacactgga cacctggctt gagacatcgt taattagcca acggcacatc
     1441 aggccaagct accctaccgc gcgtaatgtc agccccagct catcgctgcg attcaggtgc
     1501 tcaacactat tacgctctcc ggtcgccacc ttcagtgcct gacgggcgac attttctatc
     1561 gggcgcacaa tctgccattc aaaacaagcg cttgccaaca ataccactaa cacacagagg
     1621 aaatacgtca ccaccggggc agcaacaaac caaagcatgg ccgccagcaa gataaacatc
     1681 agggtcatca ctccacgcgt ccgccagcga agcggtaatg aaggcagttt acccagccag
     1741 cctttacgca ccaccaggcc tttatgaata cgcttactgg tacgtccgga gttcaacgct
     1801 ttgtacagcg gctccaccgc cgcaatctct tcatccgtcg cccgggtacg aatcgacata
     1861 tagccactga tttttccctc gcgcaccatc ggtaccgcat tggcccgcac ccaataatgg
     1921 tcgccatttt tgcggcgatt tttcacgatg ccgctccagg gctccccttt tttcagggtg
     1981 aaccacatat ccgcaaacgc cgcttttggc atatccgggt gacgcaccat gttgtgcggc
     2041 tgcccttgta actcttgcaa ggtaaagccg ctcacctgca caaaagtgtc attagcatga
     2101 gtgatatagc tttgcagatc ggtagtggac atcagagtgg tatcgtccgc cagcggggta
     2161 ttttgctggg tgacatacgg atgagaagac atgttcgcgt cctgtgcagg ttatatggtt
     2221 gttaacttct tgtcagagtt tatgtcggcc ccgctgcggt tatctttaac cgattaattt
     2281 gatttagatc gcaatttgcg attaaagcac aaatctaatt ctttgattta aaatactttc
     2341 actctgttac tatacgaaaa cgttaattat cttgcccaaa aatcaggcaa ttattgccct
     2401 gaaaacgtgc atttgcgcag caatcatcaa atccataccc gacaaaaacc gtgcaaaata
     2461 acaacaaatg ttaacggata gcattaaata ttgcacaaat gataaccgca tttgtgttta
     2521 tcccgatttt cgcgatcgca gccggagtgg cgcaatccct gcaatactta aatcggtatc
     2581 atgtgatacg cgagcctccg gagcatattt tgaacaggtt accttcgagc gcatcggctt
     2641 tagcgtgcag cgcccacgcc ctgaatctca ttgagaagcg aacgctggat catgaggaga
     2701 tgaaagcact taaccgagag gtgattgaat acttcaaaga gcatgtcaat ccggggtttt
     2761 tagagtatcg caaatctgtt accgccggcg gggattacgg agccgtagag tggcaagcgg
     2821 gaggtttaaa tacgcttgtc gacacccagg gacaggagtt tatcgactgc ctgggaggtt
     2881 ttggaatttt caacgtgggg caccgtaatc cagttgtggt ttccgccgta cagaatcaac
     2941 ttgcgaaaca accactgcac agccaggagc tactcgatcc gttacgggct atgctggcga
     3001 aaacccttgc tgcgctaacg cccggtaaac tgaaatacag cttcttctgt aatagcggca
     3061 ccgagtccgt tgaagcggcg ctgaagctgg cgaaggctta tcagtcgccg cgcggcaaat
     3121 ttacttttat tgccaccagc ggcgtgttcc acggtaaatc acttggcgcg ctgtcggcca
     3181 cggcgaaatc gaccttccgc aaaccgttta tgccactgct gccagggttc cgtcatgtgc
     3241 cgtttggcaa tatcgaagcc atgcgcacgg ctcttaatga gtgcaaaaaa accggtgatg
     3301 atgtggctgc ggtgatcctc gaaccgattc agggcgaagg tggcgtaatt ctgccgccgc
     3361 cgggctatct caccgccgtg cgtaagctgt gcgatgagtt cggcgcactg atgatcctcg
     3421 atgaagtaca aacgggcatg gggcgcacgg gcaagatgtt tgcctgcgaa catgagaacg
     3481 ttcagccgga cattctgtgt ctggcaaaag ccctcggcgg cggcgtgatg ccgattggcg
     3541 cgaccatcgc cactgaagag gtgttttcag ttctgttcga caacccattc ctgcatacca
     3601 ccacctttgg cggcaacccg ctggcctgtg cggcggcgct ggcgaccatc aatgtgttgc
     3661 tggagcagaa cttaccggct caggctgagc aaaaaggcga tatgttgctg gacggtttcc
     3721 gtcaactggc gcgggaatat cccgatctgg tacaggaagc gcgtggtaaa gggatgttga
     3781 tggcgattga gtttgttgat aacgaaatcg gctataactt tgccagcgag atgttccgcc
     3841 agcgcgtact ggtggccgga acgctcaata acgccaaaac gatccgcatt gaaccgccac
     3901 tgacactgac cattgaacag tgtgaactgg tgatcaaagc ggcgcgtaag gcgctggcgg
     3961 ccatgcgagt aagtgtcgaa gaagcgtaat atcaaatcgg atggcgatac gacgtcgcca
     4021 tccgatcttt ttatacgacg cgtacgcccg caggcatcat ccgctccggc gttaacaaca
     4081 cgctttcact gccgtcatcg gtttccgcac acaacagcat gcattccgac gtttcaccac
     4141 gcattttcgc tttttgcaga ttgcataaga ccaccaccgt tttccccatc agctcctctt
     4201 cgctgtagta cggcaccagg ctggtcacgg tttgcagtgt tttttcccca catcaacctg
     4261 tacgatgtac agcttgtcgg cgttttcatg gcgtttcact tccacaatct ttccgacgcg
     4321 catttccaga cgtgcaaaat cagcgtaagc cacggtttcc attgcttcct cccttcgagt
     4381 aaaattttac taaactatag aaaagttttt ctcaatcctg taggctaaaa atggagaatg
     4441 caggcgtgat cacattccta agccgctgtg ttaccgttac agcgtcaaag aaacgcgctt
     4501 tatttactga aaacaggtga cccgataagc acttcctcta caatgggggc gcacatcagg
     4561 gaaagtaaaa aaggtaaaca tggcaacatt aaaagacatc gcaatcgaag ctggcgtatc
     4621 cctggcgaca gtatccaggg tcttaaatga cgatccgaca ttgaatgtga aagaagagac
     4681 gaaacatcgc attctcgaga tcgccgaaaa gctggagtac aagaccagta gcgcccgtaa
     4741 actacagaca ggcgcagtca accaacacca tattctggct atctacagct accagcagga
     4801 gctggagatc aacgatcctt actatctggc gatccgccac ggcattgaaa ctcagtgcga
     4861 aaagctggcg atcgaactga ccaactgtta tgaacacaac ggattaccag acattaaaaa
     4921 cgtcaccggt attttaattg tcggcaaacc cacgcccgcc ctgcgcgccg ctgccagcgc
     4981 gttgaccgac aatatctgtt ttatcgactt tcacgaaccc ggcagcggtt acgatgcggt
     5041 ggatatcgat ctggcacgca tcagtaaaga aatcatcgac ttctatatca accagggcgt
     5101 taatcgtatt ggttttattg gcggtgaaga tgagcctggc aaggcggata ttcgtgaggt
     5161 cgcctttgcg gaatatggcc gactgaaaca agtggtacgc gaagaggata tctggcgcgg
     5221 cggtttttcc agttcgtcgg gttatgaact ggcaaaacaa atgctggcgc gggaagacta
     5281 tccgaaggca ctgtttgttg cttccgattc cattgctatc ggcgtactgc gggcaattca
     5341 tgaacgtggc ctgaacatcc cacaagatat ttcgcttatc agcgttaacg atatccccac
     5401 cgcgcgattt acctttccgc cgctctccac cgtgcgcatc cattccgaga tgatgggaag
     5461 tcagggcgtt aacctggtgt atgaaaaagc ccgcgatggt cgcgcgctgc cgctgctagt
     5521 cttcgttccc agcaaattaa aactgcgcgg cacgacccgt taaatcccct tacacactgt
     5581 tcggcaatca tttttgccgg acagtgctgc cgtttatttt cgtgatccag ttaaagtaaa
     5641 tgcatttacc tgctgctttt tagtaaaaat tttactaaac ccccagcaat tacacaaact
     5701 accatcacca tgaatggttc cgatttctct ctaccgggag gccctatgaa tcgctgggaa
     5761 aacattcagc tcacccacga aaaccgactt gcgccgcgtg cgtacttttt ttcatatgat
     5821 tctgttgcgc aagcgcgtac ctttgcccgc gaaaccagca gcctgtttct gcccttaagc
     5881 agtcagtgga atttccactt ttttgaccat ccgctgcaag taccagaagc cttcacctct
     5941 gagttaatgg ctgactgggg gcatattacc gtccccgcca tgtggcaaat ggaaggtcac
     6001 ggcaaactgc aatataccga cgaaggtttt ccgttcccca tcgatgtgcc gtttgtcccc
     6061 agcgataacc caaccggtgc ctatcaacgt attttcaccc tcagcgacgg ctggcagggt
     6121 aaacagacgc tgattaaatt tgacggcgtc gaaacctatt ttgaagtcta cgttaacggt
     6181 cagtatgtgg gtttcagcaa gggcagtcgc ctgaccgcag agtttgacat cagcgcgatg
     6241 gttaaaaccg gcgacaacct gttgtgtgtg cgcgtgatgc agtgggcgga ctctacctac
     6301 gtggaagacc aggatatgtg gtggtcggcg gggatcttcc gcgatgttta tctggtcgga
     6361 aaacacctaa cgcatattaa cgatttcact gtgcgtaccg actttgacga agcctattgc
     6421 gatgccacgc tttcctgcga agtggtgctg gaaaatctcg ccgcctcccc tgtcgtcacg
     6481 acgctggaat ataccctgtt tgatggcgaa cgcgtggtgc acagcagcgc cattgatcat
     6541 ttggcaattg aaaaactgac cagcgccagc tttgctttta ctgtcgaaca gccgcagcaa
     6601 tggtcagcag aatcccctta tctttaccat ctggtcatga cgctgaaaga cgccaacggc
     6661 aacgttctgg aagtggtgcc acaacgcgtt ggcttccgtg atatcaaagt gcgcgacggt
     6721 ctgttctgga tcaataaccg ttatgtgatg ctgcacggcg tcaaccgtca cgacaacgat
     6781 catcgcaaag gccgcgccgt tggaatggat cgcgtcgaga aagatctcca gttgatgaag
     6841 cagcacaata tcaactccgt gcgtaccgct cactacccga acgatccgcg tttttacgaa
     6901 ctgtgtgata tctacggcct gtttgtgatg gcggaaaccg acgtcgaatc gcacggcttt
     6961 gctaatgtcg gcgatatcag ccgtattacc gacgatccgc agtgggaaaa agtctacgtc
     7021 gagcgcattg ttcgccatat ccacgcacag aaaaaccatc cgtcgatcat catctggtcg
     7081 ctgggcaatg aatccggcta tggctgtaac atccgcgcga tgtaccatgc ggcgaaagcg
     7141 ctggatgaca cgcgactggt tcattacgaa gaagatcgcg atgctgaagt ggtcgatatt
     7201 atttccacca tgtacacccg cgtgccgctg atgaatgagt ttggtgaata cccgcatccg
     7261 aagccgcgca tcatctgtga atatgctcat gcgatgggga acggaccggg cgggctgacg
     7321 gagtaccaga acgtcttcta taagcacgat tgcattcagg gtcattatgt ctgggagtgg
     7381 tgcgaccacg ggatccaggc acaggacgac cacggcaatg tctggtataa attcggcggc
     7441 gactacggcg actatcccaa caactataac ttctgtcttg atggtttgat ctattccgat
     7501 cagacgccgg gatcgggcct gaaagagtac aaacaggtta tcgcgccggt aaaaatccac
     7561 gcgctgactc gcggcgagtt gaaagtcgaa aataaactgt ggtttaccac gcttgatgac
     7621 tacaccctgc acgcagaggt gcgcgccgaa ggtgaaacgc tcgcgacgca gcagattaaa
     7681 ctgcgcgacg ttgcgccgaa cagcgaagcc cccttgcaga tcacgctgcc gcagctggac
     7741 gcccgcgaag cgttcctcaa cattacggtg accaaagatt cccgcacccg ctacagcgaa
     7801 gccgggcatt ctatcgccac ttatcagttc ccgctgaagg aaaacaccgc gcagccagtg
     7861 cctttcgcac caaataatgc gcgtccgctg acgctggaag acgatcgttt gagctgcacc
     7921 gttcgcggct acaacttcgc gatcaccttc tcaaaaatga gtggcaaacc gacatcctgg
     7981 caggtaaatg gcgagtcgct gctgacccgc gagccaaaga tcaacttctt caagccgatg
     8041 atcgacaacc acaagcagga gtacgaaggg ttgtggcagc cgaatcattt gcagatcatg
     8101 caggaacatc tgcgcgactt tgccgtagaa cagagcgatg gtgaagtgtt gatcatcagc
     8161 cgcacagtta ttgcaccgcc ggtgtttgac ttcgggatgc gctgtaccta catctggcgc
     8221 atcgctgccg atggccaggt taacgtggcg ctttccggcg agcgttacgg cgactatccg
     8281 cacatcattc cgtgcatcgg tttcaccatg gggattaacg gcgaatacga tcaggtggcg
     8341 tattacggtc gtggaccggg cgaaaactac gccgacagcc agcaggctaa catcatcgat
     8401 atctggcgca gcaccgtcga tgccatgttc gagaactatc ccttcccgca gaacaacggc
     8461 aaccgtcagc atgtccgctg gacggcactg actaaccgcc acggcaacgg tctgctggtg
     8521 gttccgcagc gcccaattaa cttcagcgcc tggcactata cccaggaaaa catccacgct
     8581 gcccagcact gtaacgagct gcagcgcagt gatgacatca ccctgaacct cgaccaccag
     8641 ctacttggcc tcggctccaa ctcctggggc agcgaggtgc tggactcctg gcgcgtctgg
     8701 ttccgtgact tcagctacgg ctttacgttg ctgccggttt ctggcggaga agctaccgcg
     8761 caaagcctgg cgtcgtatga gttcggcgca gggttctttt ccacgaattt gcacagcgag
     8821 aataagcaat gaggatcatc gataacttag aacagttccg ccagatttac gcctctggca
     8881 agaagtggca acgctgcgtt gaagcgattg aaaatatcga caacattcag cctggcgtcg
     8941 cccactccat cggtgattca ttgacctacc gtgtggaaaa tgactccgcg accgatgcgc
     9001 tatttaccgg gcatcgacgc tattttgaag tgcattacta cctgcaaggg cagcaaaaaa
     9061 ttgaatatgc gccgaaagag acattacagg tagtggaata ttatcgtgat gaaactgacc
     9121 gtgaatattt aaaaggctgc ggagaaaccg tagaggtcca cgaagggcaa atcgttattt
     9181 gcgatatcca tgaagcgtat cggtttatct gcaataacgc ggtcaaaaaa gtggttctca
     9241 aagtcaccat cgaagatggt tatttccata acaaataaca actacggcgg caaaaggagt
     9301 ttgccgccac cgctacccta cacattttcg gagatgtgtt atgtctgata ccaaacgtaa
     9361 tacaatcggc aaattcggct tgctctcgct gacttttgcc gccgtttaca gctttaacaa
     9421 cgttatcaat aataatattg agcttggact ggcctcggca ccgatgtttt tcctcgcgac
     9481 gattttttat tttattccct tctgtctgat catcgcagaa tttgtttcgt taaataaaaa
     9541 ctcagaagcc ggtgtctacg cgtgggtaaa aagttcgctg ggcggacgtt gggcatttat
     9601 tactgcctac acttactggt tcgtaaatct gttctttttc acctcgctgt tgccgcgcgt
     9661 tattgcttat gcttcgtatg ccttcctcgg ctacgaatat attatgacgc cggttgccac
     9721 caccgttatc agtatggtgc tgttcgcctt ctccacctgg gtttccacca acggggcgaa
     9781 aatgctgggg ccaattacct ccgtcacttc aacgctgatg ctgctgttaa cgctctccta
     9841 cattttactg gcaggtacgg cactggttgg cggcgtacag cctgccgatc cgatcaccgt
     9901 tgacgcgatg atcccgaact tcaactgggc gttcctcggc gttaccacct ggatctttat
     9961 ggccgcaggt ggcgcggagt ccgtcgcggt gtacgttaac gacgtcaaag gcggttcgaa
    10021 atcgttcgtt aaagtgatca tcctcgccgg gatttttatc ggcgtactgt attccgtctc
    10081 ctcggtgctg attaacgtct tcgtcagcag caaagagttg aaatttaccg gcggatcggt
    10141 gcaggtattc cacggcatgg cggcgtattt tggtctgccg gaagcgttga tgaatcgctt
    10201 tgtcggtctg gtgtccttta ccgcaatgtt cggttccctg ctgatgtgga ccgcaacgcc
    10261 ggtgaaaatt ttcttctccg aaatcccgga aggcatcttt ggtaagaaaa ccgtcgaact
    10321 taacgaaaac ggcgttccgg cgcgcgcagc atggatccag tttctgatcg tcatcccgct
    10381 gatgattatc ccgatgctcg gttccaatac cgtgcaggat ctgatgaata ccattattaa
    10441 tatgaccgcc gcagcgtcca tgcttccgcc gttattcatc atgctggctt acctgaattt
    10501 acgcgccaaa ttagatcacc tgccacgcga tttccgtatg ggctcccgcc gcaccggtat
    10561 tatcgttgtt tcaatgctga ttgcgatatt tgccgtaggg tttgtcgctt cgacattccc
    10621 gactggcgcg aatattctga ccatcatttt ttataacgtc ggcggtattg ttatcttcct
    10681 cggctttgcg tggtggaaat acagcaaata tataaaggga ttaacggctg aagagcgcca
    10741 tattgaagcg acgccagcca gcaatgttga ttaagcagac taataaaaaa tgagcaggca
    10801 ttttccctct tcctggatga ggagagggaa aatagtttct gcctttatat ttttataatt
    10861 acaacgataa aaggctgtac tttttcttta gctcatggat taacacaatg aaattaatca
    10921 ctgcaccatg cagagcatta cttgctctgc cgttttgcta cgccttttct gcggcaggag
    10981 aagaagcacg tccggcagaa catgacgaca caaaaacacc cgcaattacc tcgacatctt
    11041 ctccttcatt tcgtttttac ggcgaattag ggcttggtgg atatatggat ttagagggtg
    11101 agaataaaca taaatacagc gacggcacct atattgaagg tggcctggag atgaagtacg
    11161 gctcctggtt cggcctgatt tacggcgaag gctggaccgt gcaggccgac cacgacggta
    11221 atgcctaggt gcctgaccat agctggggtg gtttcgaagg tgggattaac cgtttctatg
    11281 gcggttatcg taccaatgat ggcaccgaaa tcatgctcag tctgcgtcag gattcctcgc
    11341 tggatgacct gcaatggtgg ggcgacttca cacccgattt gggctacgtc attcccaata
    11401 cccgcgacat tatgaccgcg ctgaaggtac agaacttaag cggcaacttt cgttatagcg
    11461 tcaccgccac acctgccgga catcatgacg aaagcaaagc ctggctacat tttggcaagt
    11521 acgatcgcta cgacgacaaa tacacctatc cggcaatgga tgaacggtta catccagtat
    11581 gaccttgccg aaggcatcac ctggatgaat ggtctggaaa ttaccgacgg cacaggacaa
    11641 ctctatctca cgggcctgtt tacccctaac tttgccgctc gcgcctggca ccataccgga
    11701 cgtgccgacg ggctggacgt accgggaagt gaaagcggga tgatggtgag cgccatgtat
    11761 gaagcgttaa aaggcgttta tctctccacc gcttacacct acgccaaaca tcgccctgac
    11821 cacgctgacg atgaaaccac ctctttcatg cagtttggta tctggtacga atatggcggc
    11881 ggacgtttcg ccacggcttt tgatagccgc ttctacatga aaaatgcctc taacgatccc
    11941 agcgaccaaa tcttcctgat gcaatatttc tactggtaat aaggactgta atctcatgaa
    12001 aatcaaaact attttaacgc cagtaacctg cgctctgctg ataagttttt ccgctcatgc
    12061 cgctaacgcc gacaattata aaaacgtgat taaccgtact ggtgcgccgc agtacatgaa
    12121 ggattacgat tacgacgatc accagcgttt taatccgttt ttcgatctcg gagcctggca
    12181 tggtcatctg ttgccagacg gcccgaacac catgggcggc tttccgggcg ttgcgctgct
    12241 gacggaagaa tacatcaact ttatggccag caatttcgac cgcctgactg tctggcaaga
    12301 cggcaagaaa gtcaacttca cgctggaggc atacagtatt cccggcgcgc tggtgcaaaa
    12361 actgacagca aaagatgtgc aggtcgaaat gactctgcgc ttcgccacgc cgcgcacgtc
    12421 actactggaa actaaaatca tcagcaataa accgctggat ctggtgtggg atggcgaact
    12481 gctggaaaaa ctggaagcga aagaagggaa accgctttcc gataaaacca ttgctggcga
    12541 ataccctgac tatcagcgca aaatcagcgc cacccgtgat ggcctgaaag tcacctttgg
    12601 caaagtgcgc gccacctggg atctgctgac ctccggcgaa tcggaatatc aggtgcataa
    12661 atccctgccg gtgcagactg aaatcaacgg taatcgcttt accagtaagg cgcatatcaa
    12721 cggttcgacc acgctctaca ccacctattc ccatctgctg accgctcagg aagttagcaa
    12781 agagcaaatg cagatccgcg atattctggc gcgtccggcg ttttatctca ccgcctcgca
    12841 gcaacgctgg gaagaatacc tgaagaaagg gttaaccaat ccggatgcga cgccggaaca
    12901 gacgcgcgtc gcggtgaaag cgatcgaaac gctcaacggt aactggcgct caccgggcgg
    12961 tgcggtgaat tataacaccg tcacgccgtc ggtgaccgga cgctggttct ccggcaatca
    13021 gacctggccg tgggatacct ggaagcaggc gtttgcgatg gcgcatttca atccggatat
    13081 cgccaaagag aatatccgcg cggtcttctc ctggcagatc cagcctggcg acagcgtgcg
    13141 tccgcaggat gtgggctttg tccccgacct gatagcgtgg aaccttagcc ccgagcgtgg
    13201 tggcgatggc ggcaactgga acgaacgtaa taccaaaccc agccttgccg cctggtcggt
    13261 gatggaagta tacaacgtca cccaggataa agcctggttg gcagagatgt acccgaaact
    13321 ggtggcctat cacgactggt ggttacgtaa ccgcgatcat aacggcaacg gggtgccgga
    13381 atatggcgcg acccgcgaca aagcccacaa caccgagagc ggcgagatgc tgtttacggt
    13441 gaaaaaaggc gataaagaag agacgcagtc tgggctgaac aactacgccc gcgtggtgga
    13501 gaaaggccag tacgacagtc tggaaattcc ggcacaggtc gctgcgtcgt gggaatcggg
    13561 gcgtgatgat gccgccgtct ttggttttat cgacaaagaa cagttggata aatatgtcgc
    13621 taatggcggc aaacgtagcg actggacggt gaaattcgcc gaaaaccgca gtcaggacgg
    13681 aacgttgctg ggctactcgc tattgcagga gtcggtggat caggccagct atatgtacag
    13741 cgataaccat tatctggcgg agatggcaac catcctcggt aagccagaag aggccaaacg
    13801 ctatcgccag ttggcacagc agctcgcgga ctacatcaac acctgtatgt tcgacccgac
    13861 tacccagttc tactatgacg tacgtattga agataaacca ctggcgaacg gctgcgcggg
    13921 caaaccgatt gttgagcgcg gtaaagggcc ggaaggctgg tcgccgctgt ttaacggtgc
    13981 ggcaacgcag gccaatgccg acgcggtggt gaaggtgatg ctcgatccta aagagttcaa
    14041 cacatttgtt ccgctgggaa cggcggcgtt aaccaatccg gcttttggcg ctgatatcta
    14101 ctggcgcggg cgcgtatggg tggatcagtt ctggtttggt ctgaaaggga tggagcgtta
    14161 cggttatcgt gatgatgccc tgaagctggc ggatacgttc ttcaggcacg ccaaaggatt
    14221 aaccgccgat ggcccaattc aggagaatta caacccgctg acaggcgcgc agcaaggcgc
    14281 accaaatttc tcctggagtg ccgctcattt gtatatgttg tataacgatt ttttccgtaa
    14341 gcagtaattt gcaacggctg gcggatgcgg cgtgaacgcc ttatccgccc tacatgtgca
    14401 atcccccaag ccggataaaa cacggcaagc gtcgcatccg gcgttaccac cgggcgtatt
    14461 ctttttgaat cccatcacaa accccgcact ccccttttcc cttttctcca tcgacggcta
    14521 aattagaact catccgacta cataacaatt attttacata ctggacaatt ttatgagcta
    14581 cccgtcgctg ttcgccccgc tggatttagg ttttaccacg ttaaaaaacc gcgtgttgat
    14641 gggttcaatg cacaccgggc tggaggaata cccggacggt gctgaacggc tggctgcgtt
    14701 ttatgccgaa cgcgcccgtc acggcgtggc gctgattgtc agcggcggca ttgcaccaga
    14761 tttaacaggc gttggcatgg aaggtggcgc aatgctcaac gacgccagcc agatcccaca
    14821 ccatcgcacc attaccgaag ccgtacatca ggaaggcggc aaaatcgccc tgcaaatttt
    14881 gcataccggg cgctacagct accaaccgca tctggtcgca ccgtccgcat tgcaggcccc
    14941 catcaaccgt ttcgttcctc acgaactcac ccatgaagag atcctgcaac tgatcgacga
    15001 tttcgcccgc tgcgcgcaac tggcgcggga ggccggatac gatggcgtcg aagtgatggg
    15061 ctctgaaggg tatttgatca acgaatttct gacgctgcgc accaatcagc gtagtgacca
    15121 gtggggcggc gattaccgca accggatgcg atttgccgta gaagtagtgc gtacggtgcg
    15181 cgaacgcgtc ggcaacgact tcattattat ttaccggttg tcgatgctcg acctagtcga
    15241 aggcggcggc acttttgccg aaacggtaga actggcgcag gccattgaag cggcgggcgc
    15301 gaccattatc aacaccggaa taggctggca cgaagcgcgg attccgacca tcgccacgcc
    15361 cgtgccgcgc ggcgcattta gctgggtcac gcgcaaactg aaaggccacg tctcgctgcc
    15421 gctggtaacc accaaccgga ttaacgatcc gcaggttgcc gacgatattc tctcgcgcgg
    15481 cgatgccgat atagtatcga tggcgcgacc gtttctcgcg gatgcggaac tgctgtcaaa
    15541 agcgcaatca gggcgagccg atgagatcaa cacttgtatt ggctgcaacc agggctgcct
    15601 cgatcagatc ttcgttggca aagtcacctc gtgcctggtg aatcctcgcg cctgccacga
    15661 aaccaaaatg ccaatccttc ccgccgtgca gaaaaaaaat ctggcggtgg tcggcgctgg
    15721 acctgctggg ctggcgtttg ccattaacgc ggcggcgcgt gggcatcagg taacattgtt
    15781 tgatgcgcac agcgagatcg gcgggcagtt taatatcgcc aaacagatcc ccggcaaaga
    15841 ggagttttac gaaacgctgc gctactaccg ccggatgatc gaagtgacgg gcgtgacgct
    15901 gaaactcaat cacaccgtga cggcggatca gttacaggcg ttcgatgaaa cgatcctcgc
    15961 cagtgggatc gtgccgcgtg ttccgcctat cgacgggatc gatcatccga aggtattgag
    16021 ttatctcgat gtactgcgcg acaaagcgcc ggtaggcaac aaggttgcca tcatcggttg
    16081 tggcgggatt ggttttgata cggcgatgta tttaagtcag ccgggcgaat ccaccagcca
    16141 gaatatcgcc aggttctgta atgagtgggg gatcgacagc agcctacaac aggctggtgg
    16201 cttaagcccg cagggaatgc agatcccgcg tagcccacgg caaattgtga tgctccagcg
    16261 caaagccagc aaaccgggac aggggttagg caaaaccacc ggctggatcc atcgcaccac
    16321 cctgctctcg cggggtgtga aaatgatccc aggcgtaagt tatcagaaga ttgacgatga
    16381 cgggctgcat gtggtgatca acggcgaaac gcaggtatta gcagtggaca atgtggtgat
    16441 ctgcgcaggg caagagccaa accgcgcgct ggcgcaaccg ctgattgata gcgggaaaac
    16501 tgtgcattta attggcggct gcgatgtggc tatggagctg gacgcacgac gggcaattgc
    16561 ccagggaaca cggctggcgc tggagattta aatcgttatt tgaagcgccg gatgcaacgc
    16621 attcggcacg tcgcgttaat caataaacaa ggcgggagaa atgccgaatc gcgttgccaa
    16681 ttttttagcg tgttccagcg ttaatttcct cttcccgctc aaaacgcgtg acaccataga
    16741 tttactgcca atttccggca gatcggaaag ggttaaacca tattgatcca taagggtacg
    16801 aatcacggct ataccgccag gcatggcttg agccatggca ttaaattccg caaattcggg
    16861 cgctgattct tcccacgcgg ttattttggc acacaccaga tccagcaagg ggttttcagg
    16921 atcgttgagc agcagatgat ctaccagttc cagcgcctgg gtgtattgtt cctcgttctg
    16981 aatacccgcc agaaaaggtg ccacagcagt tagcttttct cctgcttgca agatgtcggc
    17041 aatcgcaatc attttttccc cttagtacga tgaacagcgg taaagaaatc gtattcttta
    17101 tgcgtcataa cttcacgtat gtagcacttt tgcgattcaa aaaagaccat tgctacaaca
    17161 cgtaattcat tgcccccaac attgaaaaca taatgcttat ccagatattt gaagttatcc
    17221 agagatggga atactgcttt taatgactca ggttttttga aatatccctt agcaatcgtg
    17281 ttccccagag ccaccaactc cgttttatgt tgcgggtatt tttccgcagc atctttcaat
    17341 gctttttgag ttatcaggtg cattcttcat cacgtccgtt gacaaattgg caatatgata
    17401 acatccgttg acagattggc aacggatgaa tttatctgtg tgacggaaga ataaacgtag
    17461 aaaaacgcta aacaagagaa tgtagaggat ggtgacaggg attttttgtt ttatggaagc
    17521 gcgattccag aactgagcag acattgccgg atgcgacgct tgccgcgtct tatccggcct
    17581 gggggtaacg tgctgaacgt aggccggata aggcgtttac gccgcatccg gcaggtaact
    17641 cccaatctta ccgacgacgt cccagcttca ccgctttcag caccacaaat ttattattcg
    17701 tcgcaatagt ggtgcagttg ccgaaaattt tcttcagttt atggaagtaa tccagatggc
    17761 ggttggcaac gatatacagc tcgccgttga ttttcaggca gcggcgggcg tagtggaaca
    17821 tctcccaggc gacgttatcg gtcagcgcat gttgttggtg aaacggaggg ttgcagagca
    17881 cagcattaaa gcggaaaggc tccacgccgg agagcgcgtt gttgatcata aactcgctgc
    17941 gatccaacgc ctctggcatg ttggtttcaa cgttcaaacg gctggaagca accgccatcg
    18001 gcgattcatc gacaaacacc actttcgcct gcgggttttt atcaagcagc gtcagaccaa
    18061 taacgccatt accgcaaccg agatcgacaa tctccccttc gagattctct ggcagatgtt
    18121 gcataaagaa gcgcgcgccg atatcaagcc cggtgcggga gaagacattc gcatggttat
    18181 ggatagtcca gtcagtacct tccagcttcc agctaacggt ctgcggcgca tcggccagcg
    18241 gcggttcatt gaacgtgcaa ttaatcaggc gcgctttttt ccatgccagc gtggtggtgg
    18301 tcgggccgag cactttttcg aacagttcca gcgtggaagt gtgaatgtca cgggctttgg
    18361 caccggcaat aatacgtgta tccgacgtga ccactttgcg cagcgcacgc agttgctgtt
    18421 ccagcaatgc cagtgttttc ggcactttga tcagcaccac acccggctgt tgcgggtagt
    18481 cggcggtgct gtcgagaaac ttcacgctcg actcatcaat cccgtttagg cgtaaattct
    18541 cgcgcgtcgc cagttcactg atgtatgagt cgccaatgct gtacggctta tgttccgcca
    18601 gcgcgcagct taacgcacca aaggcatcat tcaggatcaa caccgggccg aggatttctg
    18661 tgtcgtccaa ctgttgcagc aaatattcat ctgccgcttc ccacgcctgt agcgggttaa
    18721 cgtcatccgt cgccggaaaa cgttgtagtg tcagtgaacg gaaaccgttg tctaagtggc
    18781 tcatcggccc tcctgaatga taaaatttcg acgttgcccc tgaaaagggt gcgtgagtat
    18841 accctttttc tctttttcgt gggcggttat gagcaatctt acttatcttc agggttatcc
    18901 cgagcagctg ctttcgcagg tgcgaacact gattaacgaa cagcgtctgg gcgatgtgct
    18961 ggcaaaacgc tatccgggaa cacacgacta cgccaccgat aaagctctct ggcaatatac
    19021 tcaggatctg aaaaatcagt ttctgcgtaa tgccccgccg atcaataaag tgatgtatga
    19081 caataagatc catgtgctaa aaaacgcgct cgggttacat accgctgttt ctcgtgtgca
    19141 gggcagcaag ctgaaagcaa aagcagagat ccgcgtcgct accgtgtttc gcaacgcgcc
    19201 ggaaccgttt ttgcgcatga tcgtggtgca cgagctggcg cacctgaagg agaaagagca
    19261 taacaaagcg ttctatcaat tgtgctgcca tatggagccg cagtaccacc agcttgagtt
    19321 cgacacccgg ctgtggctaa cgcagttgtc gcttgggcag gacaaaatct gaaaatgatg
    19381 tcaggcgtta tcttttcttt ctttagcgcc taaaatcgac ctcccccctt tcgttaagga
    19441 taacgaccat gctgcgcgca tttgcccgcc ttcttctccg tatttgtttt tctcgccgga
    19501 ctctaaagat tgcctgctta ctgttacttg tggcaggagc gaccattttt atcgccgatc
    19561 gcgttatggt caacgccagc aagcagctga cctggggcga tgtcaacgcc gtcccggcgc
    19621 gtaacgtggg tttattgctg ggggcgaggc cgggtaatcg ctactttacc cgacgtattg
    19681 ataccgcagc agcgttgtac cacgccggaa aagtgaaatg gctactggtg agtggcgata
    19741 acggacgtaa aaattacgat gaagcatcag gtatgcagca ggctttgatc gctaaaggcg
    19801 taccggcgaa agtgatcttt tgcgactatg ccggattctc aacgctggat tcggtagtgc
    19861 gtgccaaaaa ggtgtttggg gaaaaccata tcaccattat ctcgcaagaa tttcataacc
    19921 aacgcaccat ctggctggca aaacagtacg gcatcgacgc tatcgggttt aacgcgccag
    19981 acctcaatat gaagcacggt ttttatactc aactgcggga gaaactcgct cgtgtcagcg
    20041 cggtgattga cgcaaaaatc ctccatcgcc agccgaaata tctcgggcca tcggtcatga
    20101 ttgggccgtt tagtgagcat ggctgtccgg cgaaagaata atgcgtatct gcgcacgtcg
    20161 aagatgaaaa aggcgtgcta cattgacgac agaatccctt tatggagtat ccacgcgtta
    20221 tgatacgttt cgctgtgatt ggtacgaact ggatcactcg ccagttcgtc gaggccgccc
    20281 atgagagcgg taaatacaag ttaaccgccg tatattcccg cagccttgag caggctcagc
    20341 acttcgccaa tgatttttct gtcgagcatc tgtttacgtc gctggaagcg atggcggaaa
    20401 gcgatgccat tgacgcggtg tatattgcca gcccgaattc cctgcacttt tcccagacac
    20461 aacttttcct tagccataaa attcatgtga tttgcgaaaa accgctggcg tcgaatctgg
    20521 cggaagtgga tgccgccatt gcctgtgcgc gcgaaaatca ggtggtgctg tttgaggcat
    20581 ttaaaaccgc ctgcctgccg aactttcatt tgttgcgcca ggcgctgccg aaagcaggca
    20641 aactgcgtaa agtctttttc aactattgcc agtattcctc gcggtatcaa cgttacctgg
    20701 acggtgagaa tcccaacacc tttaatccgg cattctctaa cggttcaatt atggatatcg
    20761 gcttttactg cctggcgtcg gcggtggcgt tatttggtga gccgaaaagc gtgcaggcaa
    20821 cggcgagttt gctggcaagc ggcgttgacg cccacggcgt ggtggtgatg gattacggtg
    20881 atttcagcgt caccttgcag cactcaaagg tcagtgattc tgtcctggga agcgagattc
    20941 agggcgaagc gggatcgctg gtgattgaaa aactgtctga atgccagaaa gtctgctttg
    21001 tgccgcgtgg cagccagatg caggatctca cccagccgca gcatattaat accatgctct
    21061 acgaagcaga gctgttcgct accctggtgg atgagcatct ggtggatcat ccagggctgg
    21121 cggtcagtcg catcacagcc aaactgctga ccgagatccg ccgccaaact ggggtgattt
    21181 ttccggcaga tagcgtaaaa ctatacttgc caaagtaaaa cagtgtaaaa ggtatgtaac
    21241 agaccattga ctgactgaat ggtctgtcat actttgttac ctgcaaaggg gagtaacttc
    21301 attgccggtc gatcgtcatt acgatgagtg aaaaaccaca tccggtcacc gggcaacccg
    21361 aaaggaatac gcagacgtat tccttttttg ttgtaagtga gaccttgccg gaaggcgagg
    21421 tctatgcata aaaagcagcg gctgacgtct tccgacgttg gccgtttttt tatgtgtaag
    21481 gaacttctat gaatactgtc ggcacgccgt tgctatgggg cggattcgct gttgttgtcg
    21541 ccattatgct ggctatcgac ctgttgttgc aggggcgtcg tggggcacat gccatgacca
    21601 tgaaacaggc tgcggcctgg tcgctggtct gggtgacgct gtcgttactg tttaacgccg
    21661 ctttctggtg gtatctggtg caaaccgaag gtcgcgccgt tgccgatcca caggccctgg
    21721 cctttctcac gggttatctg attgagaaat cgctggcggt cgataacgtc tttgtctggc
    21781 tgatgttgtt cagctatttc tccgttccgg cggcattaca acgtcgcgtg ctggtgtatg
    21841 gcgtgctcgg ggcgattgtt ctgcgtacca tcatgatctt cacaggcagc tggctgattt
    21901 cgcagttcga ctggatcctg tatatcttcg gtgccttcct gctgtttacc ggcgtgaaga
    21961 tggcgctcgc ccatgaagat gaatcaggca ttggcgacaa gccgctggtg cgctggctac
    22021 gcggtcattt gcgcatgacc gacaccatcg acaacgagca tttctttgtg cgtaagaatg
    22081 gattgctgta cgccacaccg ctgatgctgg tactgattct ggtggaattg agcgacgtga
    22141 tttttgccgt ggatagcatt ccggctatct tcgccgtgac cactgacccg ttcattgtgc
    22201 tgacctcaaa cctgtttgca atcctcggcc tgcgtgcgat gtatttcctg ctggcgggcg
    22261 tagcagagcg tttctcgatg ctcaaatatg gcctggcggt gattctggtg tttatcggta
    22321 tcaagatgct gattgtcgac ttctaccata ttccaatcgc cgtctcgctg ggcgtggtgt
    22381 ttggcattct ggtgatgacg tttattatca acgcctgggt gaattatcgg catgataagc
    22441 agcggggaga atgattcgct gtatattaag ccattcctag agataactca ttccagacaa
    22501 aggtgagcta ttaattatgt aatcggctta tagctcttta caaaaagacc gcacgggtgt
    22561 tggtggcgtc aaaatcgccg aagcgttccc tggaggccgg ggcgagggcg catcttacag
    22621 ggacgttacc ttgcgcccga cccggtagcc gcaagggata ggctgaggga accgcgcagc
    22681 ggcgattttg ttcgccagag cctgggggtg cagggggcgg cggcgattgg ccgccccctg
    22741 cgcgctccct gcgccagtga ctttatatgg cataacacgt taaaggagcg caaccgttct
    22801 catattccaa atagttttgc ccatatgtta tctaatcagc cttagtagcc agtccttccc
    22861 tctgtttatg ttaaataaat gcaaccatta agttgcaaaa catgacctct ctttaaaatc
    22921 cagcattttt cgcttcccga agctgtaact ttccttatac tcgaccttgc aaacactttg
    22981 ttacatcctg aaagatgcgt cgacagaacg caccagggat gtgcgacaac acaatgaaag
    23041 gatcgaaaaa tgactacgca acgttcaccg gggctattcc ggcgtctggc tcatggcagc
    23101 ctggtaaaac aaatcctggt cggccttgtt ctggggattc ttctggcatg gatctcaaaa
    23161 cccgcggcgg aagctgttgg tctgttaggt actttgttcg tcggcgcact gaaagctgtt
    23221 gcccccatcc tggtgttgat gctggtaatg gcatctattg ctaaccacca gcacgggcag
    23281 aaaaccaata tccgtcctat tttgttcctc tatctgctgg gcaccttctc tgcagctctg
    23341 gccgcagtag tcttcagctt tgccttccct tctaccctgc acttgtccag tagcgcgggt
    23401 gatatttcgc cgccgtcagg cattgtcgaa gtgatgcgcg gactggtaat gagcatggtt
    23461 tccaacccca tcgacgccct gctgaaaggt aactacatcg ggattctggt gtgggcgatc
    23521 ggcctcggct tcgcactgcg tcacggtaac gagaccacca aaaacctggt caatgatatg
    23581 tcgaatgccg ttacctttat ggtgaaactg gtcattcgct tcgcaccgtt cggtattttt
    23641 gggctggttt cttctaccct ggcaaccacc ggtttctcca cactgtgggg ctacgcgcaa
    23701 ctgctggtcg tgctggttgg ctgtatgtta ctggtggcgc tggtggttaa cccattgctg
    23761 gtgtggtgga aaattcgtcg taacccgttc ccgctggtgc tgctgtgcct gcgcgaaagc
    23821 ggtgtgtatg ccttcttcac ccgcagctct gcagcgaaca ttccggtgaa tatggcgctg
    23881 tgtgaaaagc tgaatctgga tcgcgatacc tattcagttt ctattccgct gggagccacc
    23941 atcaatatgg cgggcgcagc aatcaccatt accgtgttga cgctggctgc ggttaatacg
    24001 ctgggtattc cggtcgatct gcccacagcg ctgctgttga gcgtagtggc ttctctgtgt
    24061 gcctgtggcg catccggcgt ggcggggggt tctttgctgc tgatcccact ggcctgtaat
    24121 atgttcggta tttcgaacga tatcgccatg caggtggttg ccgtcggctt tattatcggc
    24181 gtattgcagg actcttgcga aaccgcgctg aactcttcaa ctgacgtgct gttcactgcg
    24241 gcagcttgcc aggcggaaga cgatcgtctg gcaaatagcg ccctgcgtaa ttaattgttt
    24301 aacccctttc gtctatggcg ggaggggttt tctctacttt aaacggatca attccccttt
    24361 tctgcatccg ccagaaacga atgatattca ggccattcat aagcagaaaa ctaccctcaa
    24421 tcatcgtgcc gcctatcgac cccgcccaga agttgtgaat cacccagcaa cacgttgaaa
    24481 accacatcac gcagcgcatg gtgagccctt tacagcggaa cagcgcccag gtactgacaa
    24541 tcgtaccgat aaccggcaat agttcgacag gatgatggaa cttcgcgagg ccaattccgc
    24601 cagtcagcac aataaaaatc gccattaccc ataagctgcg cgtgcgtaag gtaatcaatg
    24661 tacgaatggc attaaggatg gcactggcac cagcgggata ggtgcccaga agaaaaaaat
    24721 gtacgccaat aacggcgcta tagaccgaaa gctgcttttt gaagcgacgt tcgtcacgat
    24781 tgaaaaatgt tgtgatacca atcagaaagg cgatgacacc cacgccctgg gccagccaat
    24841 acgcggtcat gataaatcct tagcaggtat ggaaaagcaa acggcgcttc acattatgaa
    24901 acgccgtttt ttattaacaa ctcatttcga ctttatagcg ttacgccgct tttgaagatc
    24961 gccagttcgc ggaagtcgtt acgctcgttg caggtttgct taccgttggc aaactcaacg
    25021 atggtgtcga taaattcttc cagcaactgc ggcatcgctt taccgtggat cagctgaccc
    25081 gcgtcaaagt cgatccagtg ttttttcttc gccgccagtt cactgttggt ggcgattttc
    25141 accgtcggca caaatccacc gtatggcgtg ccgcgaccag tactgaacag caccatatgg
    25201 cagcccgcac ccgccagggc gctggtcgct acggcatcgt tacccggcgc acttaacaag
    25261 ttcagccctg gcgttttcag acgctcgccg taacgcagca cgtcaaccac gacactggaa
    25321 cccgctttct gggtacagcc aagtgatttg tcttccagcg tggtgatacc gcccgctttg
    25381 ttccccggtg atgggttttc gtagatcggc tgatcatggg caataaagta ctgtttgaag
    25441 tcgttgacca tggtgaccag tttttcaaac gttgcttcgt cgcggcaatg gtccatcagc
    25501 aactgctctg cgccaaacat ctccggcact tcggtcagta cggtagtacc gccgttagca
    25561 atcacgtagt cagagaaacg ccccagcatc gggttagcag taataccaga aagaccgtca
    25621 gaaccaccgc actccagacc aaacttcagt tcgctgagtt tgcctggctc gcgtttatcg
    25681 ttgcgcatca cgttatacag ctgatgcaaa tgctcgatcc cggcttcgat ctcatcatcc
    25741 tgctgttggc agatcatgaa atgaacgcgt tcaggatcga tatcgcccaa cgtttcacgg
    25801 aatgcggcaa cctggttgtt ttcacagccc agaccaatca ccagcactgc gcccgcgttc
    25861 gggtggcgca ccatgttttg cagcatggtg cgggtgttga tgtgatcgtc gcccagttgt
    25921 gagcagccat aggtgtggct gaagaggaac acgccgtcgg taccttcggc gttgttggtc
    25981 tctttcagga aacggttctg gatttgccgc gcgatgccgt tgacacagcc cacggttggc
    26041 aggatccaca gctcgttacg caccccgaca tcgccgttag cgcgacgata gatctgcact
    26101 tcaggatctg ccgcttgcgc aggcagatcc tgaaaatcaa gttgatagcg atactgatcc
    26161 agatcgctca gattcgtgcg cgtattgtgg gcgtggacgt gttcccccgc cgcaatatcc
    26221 gccaatgcat aaccaatcgg caggccatat ttgatgacgt tggccccttt tgcgatatcc
    26281 gttaacgcaa atttatgtcc acgagcaaca tcctggcgca gcgtaacagt ctggttatcg
    26341 acactgactt ctgtgccttc agccaaatct gctaaagcga ccgcgacgtt atccagcgca
    26401 tggatcttga tgtattgcat atcaacccca gaccttagtt cagttcaatg gcgaagtagt
    26461 cacgcgcatt gttaaagcaa atatttttca ccatctcgcc cagcaggttg atgtccgccg
    26521 gtgcttcgcc cgcttccacc cagcgaccga tcatctggca cagaatgcgg cggaagtatt
    26581 cgtgacgggt gtatgacagg aagctacggc tgtcagtcag cataccgaca aagcggctca
    26641 gcagaccgag ctgcgccagt tgggtcatct gacgttccat accgtctttc tgatcgttaa
    26701 accaccagcc ggaaccgaac tgcattttgc ccggcatacc ttcgccctgg aagttaccga
    26761 tcatggtgcc cagcacttcg ttatcgcgcg ggttcaggca gtagagaatg gttttcggca
    26821 gcaggttttc ttcgttctgc ttgctcagca gcttagacaa ctcttccgcc atcggacggt
    26881 cgttgatgga gtcaaagcct acatccggcc ccagcagttt gaactgacgc aggttgttat
    26941 tacgcagcgc gccaatgtgg tactgctgta cccagccgcg acgtgcatat tcggcaccaa
    27001 ggaacaccag taccgcagtt ttgaactgtg ccacttcgtg ctcgctcagg gtttcgccag
    27061 ccagacggcg cgccagaatg ctgtccagtt ccgcttcgtt cgcttcagca aacatcacca
    27121 cgtccagcgc atggtcagac actttacagc cgtgagcggc gaagtgatcc agacgtttgg
    27181 tcaaggcagt ttgcaggtca gcaaagcggc gaatgtcggt atcggaaact tcgcccagct
    27241 tcgccatgta gtcgttaaag gtcgcctgtt cgatattgaa ggctttgtcc ggacgccagc
    27301 tcggcagcac tttgatggtg aaagagccgt ctttggcgat ctctgcgtga tgctccagag
    27361 aatcgatcgg gtcatcggtg gtgccgacca ttttcacgtt catctgctgc atgatgccgc
    27421 gcgcggagaa gttatcctgc gccagcagtt cgttacattc gttccagatt tcatcggcag
    27481 ttgacggaga aagcaatttg ccagtgatac cgaacggacg acgcagttcg aggtgcgtcc
    27541 agtggtataa cgggttgccg atagtgtgcg gaacagtcgc cgcccaggcg tcaaattttt
    27601 cacggtcaga cgcatcacca gtacacagac gctcagccac accgttggta cgcatagcgc
    27661 gccatttgta gtgatcgccc ttcaaccaga tgtcatacag gtttttaaaa cgatagtctt
    27721 ccgcaatctg ctgcggcggc aaatggcaat ggtaatcgaa aatcggctgg tcttttgcgt
    27781 agtcgtgata cagacggcgg gcaaattcgg tatctaacag gaaatcttca gtcataaacg
    27841 gagtcatttt cgtcttcctc tcaacgagtg agctagcttg cttatggtgc gatgctgaca
    27901 aagttatcac accaatttcc agagtccgaa gatattttcg tgagttagat caataaacgt
    27961 agttaaaaaa attactctca aagtggtaaa tctcgctgca ggccgcgcca gtactggcct
    28021 cgctgtcgtc aggtaatgtc cctacaaata ttcgcacatt tgtgatggct ctcacctttt
    28081 aaggttgtat gacaagttat ctttctgccg tcgcaaatca taagtcgacg gaatgcaaat
    28141 tgccgattca ttcatttgtt agatgaatcg ggttaaccgg tacggaagcc gaattagcac
    28201 gaaactttca tggcaacgtt c
//
LOCUS       DAZXUK010000023        28168 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZXUK010000023 DAZXUK010000000
VERSION     DAZXUK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28168
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.023"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACH3L8_000831"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACH3L8_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFT4084065.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="ACH3L8_000832"
     CDS             complement(2138..2584)
                     /locus_tag="ACH3L8_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFT4084066.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="ACH3L8_000833"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="ACH3L8_000833"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFT4084067.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACH3L8_000834"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACH3L8_000834"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFT4084068.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="ACH3L8_000835"
     CDS             complement(5347..6009)
                     /locus_tag="ACH3L8_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFT4084069.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="ACH3L8_000836"
     CDS             6182..6595
                     /locus_tag="ACH3L8_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFT4084070.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACH3L8_000837"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACH3L8_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFT4084071.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACH3L8_000838"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACH3L8_000838"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFT4084072.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="ACH3L8_000839"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="ACH3L8_000839"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFT4084073.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACH3L8_000840"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACH3L8_000840"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFT4084074.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACH3L8_000841"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACH3L8_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFT4084075.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="ACH3L8_000842"
     tRNA            complement(9861..9948)
                     /locus_tag="ACH3L8_000842"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="ACH3L8_000843"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="ACH3L8_000843"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFT4084076.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="ACH3L8_000844"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="ACH3L8_000844"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFT4084077.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="ACH3L8_000845"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="ACH3L8_000845"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFT4084078.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /gene="hyaD"
                     /locus_tag="ACH3L8_000846"
     CDS             14005..14592
                     /gene="hyaD"
                     /locus_tag="ACH3L8_000846"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFT4084079.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="ACH3L8_000847"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="ACH3L8_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFT4084080.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="ACH3L8_000848"
     CDS             14984..15841
                     /locus_tag="ACH3L8_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFT4084081.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="ACH3L8_000849"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="ACH3L8_000849"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFT4084082.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="ACH3L8_000850"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="ACH3L8_000850"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFT4084083.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="ACH3L8_000851"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="ACH3L8_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFT4084084.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="ACH3L8_000852"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="ACH3L8_000852"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFT4084085.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACH3L8_000853"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACH3L8_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACH3L8_000854"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACH3L8_000854"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFT4084086.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="ACH3L8_000855"
     CDS             complement(22884..24023)
                     /locus_tag="ACH3L8_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFT4084087.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="ACH3L8_000856"
     CDS             complement(24069..26165)
                     /locus_tag="ACH3L8_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFT4084088.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="ACH3L8_000857"
     CDS             complement(26165..26911)
                     /locus_tag="ACH3L8_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFT4084089.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACH3L8_000858"
     CDS             complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACH3L8_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFT4084090.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="ACH3L8_000859"
     CDS             complement(27659..27964)
                     /locus_tag="ACH3L8_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084091.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT         6765 a         7189 c         7448 g         6766 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagacaaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatctta
//
LOCUS       DAZXUK010000024        27362 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZXUK010000024 DAZXUK010000000
VERSION     DAZXUK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27362
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.024"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..576
                     /locus_tag="ACH3L8_000860"
     CDS             <1..576
                     /locus_tag="ACH3L8_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4084092.1"
                     /translation="LAEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNEWFGLC
                     MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLD
                     NLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEK
                     GSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            812..1630
                     /gene="ybhA"
                     /locus_tag="ACH3L8_000861"
     CDS             812..1630
                     /gene="ybhA"
                     /locus_tag="ACH3L8_000861"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFT4084093.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACH3L8_000862"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACH3L8_000862"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFT4084094.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACH3L8_000863"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACH3L8_000863"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFT4084095.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACH3L8_000864"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACH3L8_000864"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFT4084096.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACH3L8_000865"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACH3L8_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFT4084097.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="ACH3L8_000866"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="ACH3L8_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFT4084098.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="ACH3L8_000867"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="ACH3L8_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFT4084099.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="ACH3L8_000868"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="ACH3L8_000868"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFT4084100.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="ACH3L8_000869"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="ACH3L8_000869"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFT4084101.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="ACH3L8_000870"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="ACH3L8_000870"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFT4084102.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="ACH3L8_000871"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="ACH3L8_000871"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFT4084103.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="ACH3L8_000872"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="ACH3L8_000872"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFT4084104.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="ACH3L8_000873"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="ACH3L8_000873"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFT4084105.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13936..14316
                     /locus_tag="ACH3L8_000874"
     CDS             13936..14316
                     /locus_tag="ACH3L8_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFT4084106.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="ACH3L8_000875"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="ACH3L8_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFT4084107.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="ACH3L8_000876"
     CDS             complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="ACH3L8_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFT4084108.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="ACH3L8_000877"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="ACH3L8_000877"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFT4084109.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDAFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17417..17492)
                     /locus_tag="ACH3L8_000878"
     tRNA            complement(17417..17492)
                     /locus_tag="ACH3L8_000878"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17457..17459),aa:Lys,seq:ttt)
     gene            complement(17496..17571)
                     /locus_tag="ACH3L8_000879"
     tRNA            complement(17496..17571)
                     /locus_tag="ACH3L8_000879"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17536..17538),aa:Val,seq:tac)
     gene            complement(17623..17698)
                     /locus_tag="ACH3L8_000880"
     tRNA            complement(17623..17698)
                     /locus_tag="ACH3L8_000880"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17663..17665),aa:Lys,seq:ttt)
     gene            complement(17701..17776)
                     /locus_tag="ACH3L8_000881"
     tRNA            complement(17701..17776)
                     /locus_tag="ACH3L8_000881"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17741..17743),aa:Val,seq:tac)
     gene            complement(17912..17987)
                     /locus_tag="ACH3L8_000882"
     tRNA            complement(17912..17987)
                     /locus_tag="ACH3L8_000882"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17952..17954),aa:Lys,seq:ttt)
     gene            complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACH3L8_000883"
     CDS             complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACH3L8_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFT4084110.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACH3L8_000884"
     CDS             complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACH3L8_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFT4084111.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACH3L8_000885"
     CDS             complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACH3L8_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFT4084112.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACH3L8_000886"
     CDS             complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACH3L8_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFT4084113.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACH3L8_000887"
     CDS             complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACH3L8_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFT4084114.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACH3L8_000888"
     CDS             complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACH3L8_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFT4084115.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACH3L8_000889"
     CDS             complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACH3L8_000889"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFT4084116.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="ACH3L8_000890"
     CDS             complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="ACH3L8_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFT4084117.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="ACH3L8_000891"
     CDS             complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="ACH3L8_000891"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFT4084118.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="ACH3L8_000892"
     CDS             complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="ACH3L8_000892"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFT4084119.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="ACH3L8_000893"
     CDS             complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="ACH3L8_000893"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFT4084120.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         6887 a         7255 c         6704 g         6516 t
ORIGIN      
        1 cttgccgaag aagatccagc ttctgcaacc aggccattca gaactgaagt gaagcgcagt
       61 cgactgttaa ttgacgagta tctgttaatc aggaaaatag cagacgtcca gaacgaatgg
      121 ttcggccttt gcatggatct tgcactggta acaggacagc gcaaaggaga tctggcggct
      181 atgagatggg aggatataag agatagcagg ctatacgtgg aacaacaaaa aacaggggca
      241 aaaattcgca tttctctccc caccacgatt tccagattga atttaacact tgccgatgta
      301 ctggataact tgaaaaaaat aaacggtaaa aacgaaaagt tactcggagg gaagacagcc
      361 aggacaattg cagcacagtt caggattgcc cgagacacat ccggattgaa atgggaaggt
      421 gacccaccgc catttcatga aatccgaagt ttatccggtc gcctgcactc agcagaaaaa
      481 ggcagtgatt tcactcaggc gctccttgga catcgttcat ccagcatgac ggataaatac
      541 cgtgacggac gagggaggga atggaaagac atttagaatt aatttcgaat gatttcgaaa
      601 tgccacttgt aattaattga ttataaagaa ttaattccta aagagcattt gcttcatgaa
      661 tgctcctttg catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa
      721 tgctgaatta aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac
      781 gaaaattctt aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt
      841 agacggcacc ttattgaccc cgaaaaagac cttgcttcca tcatcgatag aagccctggc
      901 ccgcgctcgc gaagcaggct atcaattaat catcgtcaca ggtcgccatc acgtcgctat
      961 tcatcctttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattgccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc actcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag attacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggtc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgttt ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgcactct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagtgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggccga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tatgccaacc gcatcgccaa tagtcgctat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagcatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcaccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatgg gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgttgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctgga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctctcctaca atttctaact gtaactcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca caccaccatt cagatggaat
    15301 atcaaccttg tcataggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 gccgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggtca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaatgcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgcttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgtaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcga attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttgcattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17461 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17521 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17581 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    17641 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg
    17701 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    17761 gctgagctaa tcacccgaca ctacgctgga tactacaact aactatgtta gtgggggtcg
    17821 tgcaggatga ctcgacttcg tctcgccctt cggcgccgtt gctgatgcaa cgttatcctt
    17881 cacgttttac taccgcttcc accttagcga ttggtgggtc gtgcaggatt cgaacctgcg
    17941 accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccact ttttcgttgc
    18001 tttcggtttg tttgatatcc cgtggcaacg gcggcatata ttactgattt cagacttgag
    18061 cgcaacaaaa atttcgatgc agatcactca actgcttatg attcgcacga cacgaccaga
    18121 aataatgcga cttctggtcg tgtgttatgc attacatcgc gttcagacgt ttttgtgcct
    18181 gtttagcgcc atcagtacca gggtatttac tgataacctg ctggtatacg gctttcgctt
    18241 ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt aaacatcgca tcagcagcct
    18301 ttggtgactt cggatagttt ttcactaccg aagcaaaata gtacgccgca tcatcttttt
    18361 tacccttgtt gtagtttaac tgacccagcc aataattggc gtttggcagg taagttgaat
    18421 cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc gtcatcctgg cgggatttat
    18481 cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc atcgccgctt ttcaccggcg
    18541 cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt tgatgccgtc gcaccgcttt
    18601 gatcgctgct ggttgattgc gccgctgcac caccgctgct gaggctgtcg atctgcaaca
    18661 ggatctgctt ttgccgctcc acgacctgat tcagttgata ctgattttcc tgaatctgac
    18721 cacgcaggga atcaatatcg gattgattat cagaaagttg ttgctggagt tgggttaaaa
    18781 gctggctgtg agcattagaa atacgctcaa gttgagtgac gcggtcttcg accgagcctg
    18841 agctgacact actgattggt gcctgagcaa aagcggccca gggggccgct ataccaacca
    18901 gtaacgacag actcaatagt tgatgtctga agttactgct catgcaattc tcttagtaaa
    18961 ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc atgacccagt actgcaggtt
    19021 tttctttacc gtaagaaacg atggagatct ggtctgcaga aacgccttta ccctgcaggt
    19081 acatcttaac ggcgttcgca cgacgttcac ccagggagat gttgtattcc ggagtaccac
    19141 gttcgtccgc gtgaccttct acggtgactt tgtaagacgg gttgctacgc aggaagtttg
    19201 catgtgcatc cagcatttga gcgaagtcag aacggatatc gtacttgtcc agatcgaagt
    19261 aaacgatgtt gttctgctgc agctgttgca tttgcagacg agcctgctct tcggaagaca
    19321 tgttgccgtt gccgccgttc gcatccatac cagtgccggc acccagcatg ccttcgctgc
    19381 cgtcattgct ggcgttcttg ttggaagaac atgccgcaat tgccataaca ggcagagcaa
    19441 tcatcagccc tttcagcact ttgttcagtt gcatttcaat gattccttta ctattcaatt
    19501 aattattatc acagatacgg cgaccaggca gggaatttga cctgtccatc agttgccgga
    19561 agacgcgctt tgaaacgccc atctgtagaa accaaattca gcacggatcc catcccctga
    19621 gaagagctgt agattaccat agtgccgtta ggtgccagac ttggcgtttc atccaggaac
    19681 gtggacgaca gaacttgtac gcctcccgtt gccagatctt gtttggcaat gtgctgctgc
    19741 ccaccattgg agctgaccat taccataaat ttaccgtcgc tgctgacatc cgcatcctgg
    19801 ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc cgttgatatt cactttataa
    19861 acctgcggac gaccggcctg gtcagaagta aatgccaggt tctggctatc cgggaaccag
    19921 gtcggttcgg tattgttact gcgaccatca gtcacctggc ggatctgacc agaagccaaa
    19981 tccattacgt acaggttcag actaccggtt ttcgacaagg cgaatgccag tttgctgccg
    20041 tctggcgaga atgcaggtgc accgttgtga cgcgggaatg aagccacctg acgtacagcg
    20101 ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc tttcgaaggt cacataagcc
    20161 agtttagaac cgtctggtga ccacgccgga gacatcagcg gctgcggtga acggtgaacg
    20221 acaaactggt tgtaaccgtc atagtcagat acgcgcagtt catacgggaa ctgaccgccg
    20281 ttggtctgaa caacgtaggc aatacgggta cggaacgcac ctttaatgcc ggtcagtttt
    20341 tcaaacactt catcactggc ggtatgacca gcataacgca gccactgctt gttcactttg
    20401 tacgagttct gagcaagtac agtacccggt gcgccgccag tgtcaacaag ttgataagca
    20461 acattgtaag aaccatccgg attcggagtg acttgaccga caactacagc gtcaatgccc
    20521 agtgcggacc atgcagctgg ttgtacttcc tgcgcactac ccggctgctg tggcagacga
    20581 gcgcgatcta acggattaaa tttaccgctg ttacgcaagt cagcagcaac gatgccgcca
    20641 atatcttcag gtgccgcacc aggccccgcc cactggaaag gaacaacacc aataggacga
    20701 ccggaatcta caccgctgtc gatcacaatg cggacttcag catgcagaac tgatgcccac
    20761 agtatgagaa aaccaaatgc tactcgtaat gcctgcttca tcatatctcc cttatctgga
    20821 ccgatggccc acgataattt agcagaatgt taacaaacta aaatacacaa aactaccaga
    20881 accccgtggc aaccggtgcc tgatgttgac cgtccgaaca gtcaacatcg cgattacggt
    20941 ttgaagtcca atggcgcgtt tttgaacact tcatatactg cctggcttgg tggtttcggg
    21001 atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa gtgcgggatc gccaccttca
    21061 ggtttgatat ccagtaacat accatcgggt gccagtttta tgcgcagcgt acaggttttg
    21121 cctgcatagg acgatgcgtc atagaactta ctttcgatag cagatttaat ctgcccggca
    21181 tagttattga tatcggcccc tgatgcgcca ttgtttttag tattaccact cccggcaggc
    21241 gaagcattgt tccctttcgc ccctcccccc gttttcggtg cattcttacc agagcttagc
    21301 tcaccgaaaa tatcatctgc ctctgcggca gcttttgctg cagcggcttt ttcagctgct
    21361 gcttttgccg ctgccgcttt tttgtctgcg gcggcttttt ctgccgctgc tttcttatca
    21421 gctgctgcct tatcagcagc cgctttttct gctgcttttt tgtcggctgc agctttctct
    21481 gctgccgctt tcttatcagc tgcagccttt tcagcagccg ctttcttctc agcttctgcc
    21541 ttggcttttt cagcagcttc agttgccgct ttctttcttg cttcagctgc agctgcttct
    21601 gccgcttccg ctttcttctt cagtgccgca gcggctacct cggctttttt ctgcgcttcg
    21661 gctgcggctt tggcggcttc tgcttctgct tttttcttcg cgtctgcagc cgctttcttc
    21721 gctgcttctt ccgcagcttt agcatctgcc gccgctttcg ctgccgccac ttcagcttgc
    21781 ttctgcttta actcggcctg ttttgcggct tcttcagcct gctttttctg ctcctgagcc
    21841 gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt cagccgcttg tttctcacgc
    21901 agttcttcag cagcctgctg ttccttcatc ttgcgctgtt catcagaacg cttcgcgctt
    21961 gattcctggc tttgcatgcg tttgtactgc tcaactaccg cacctgaatc aaccatgaca
    22021 gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt ctatattctc atcgaacgaa
    22081 ctccagatca gcgccgcaaa taagatgaca tgcagcactg ctgaaataat tatcgcccgc
    22141 ttgagcttgt cgttttgttc ggttgccttt gacactctcg gtttccaaaa actgttcgcc
    22201 tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt tagataggct gcgtcattaa
    22261 accaaccgat ttcacacccg cactatgtaa caagttcagt gctttaatta tttcatcgta
    22321 aggcacatct tttgcgccac cgatcagaaa gaccgttttc gggttggcct tgaaacggct
    22381 ggacacttcc gccaccacct gctctggtgg taaacgctcc aggcgatctt tctcaaccac
    22441 cacggtgtac tgaccaatac cagacacttc aacaatcact ggcggattat cgttactgct
    22501 caccgcctgt gattcagtag cgtctggcag atcgacctcc acgctctggg tgatgatggg
    22561 cgctgtcgcc ataaagatca gcaacagcac cagcagtacg tccagcaacg gaacaatgtt
    22621 gatttcggac ttgagatcgc gacgacctcg tccacgcgct ctggccatgg cttacccctt
    22681 gttgctctcg ctaacggtaa acgcctggcg gtgcagaatc gcggtaaact cttccataaa
    22741 gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg agacggttat aagccattac
    22801 cgccggaatt gcggcgaaca gaccaattgc cgtcgcaatc aacgcttctg cgataccggg
    22861 cgcaaccatt tgcagtgttg cttgttttac cgccccgagg gcgataaagg cgtgcatgat
    22921 cccccagacc gtaccaaaca gaccaatata cgggctgatg gagccaaccg taccgaggaa
    22981 cggaatgtgc gtttccagat tttcaagttc acggttcatg gagatacgca tagcacgcga
    23041 cgccccttcc actacggctt ccggcgcatg gctattggca cgatgcaggc gcacaaactc
    23101 tttgaacccg ctgtaaaaga tttgttccga accagtcaga ttatcccgtt tcccctgact
    23161 ctcttgatag aggcgagaga gttcgattcc agaccagaat ttatcttcaa acgcttcggc
    23221 ttcacgcgcc gctgcgttaa gaatacgggt tcgctggata ataatggccc aggatgcgat
    23281 tgaaaaacca atcaaaatca acatgataag tttaaccaga aggctagcct tcaggaacaa
    23341 atcaaggata ttcatgtcag tcactgctta aactccgcga caatagactt gggaagcgca
    23401 cgaggcttca ttttgagtgg gtcaacgcaa acaaccagaa cctctgcttc attcagcaaa
    23461 gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag aggtgccacg cattgatgtt
    23521 atttcagtct gtatttcgag catatcgtcg agccgcgcag gtgcgtaata ttccaccgtc
    23581 attttacgta ccacaaaggc aacgcgttca gccatcagcg cctgctgact gaagtgatga
    23641 tgacgcagca tctctgtgcg tgctctttca taaaaagcga cgtaactggc gtggtacacc
    23701 acaccaccgg catcggtatc ttcatagtag acgcgaaccg gccatcgaaa cagcgttgta
    23761 ttcactttac atcccggtaa tgcaacaaaa gttagagctt ttaaacgctg ctactatacg
    23821 cgcgcgattg aggtttggga atgagggcaa gttaagggcg cgtaaaaatt tatgggccta
    23881 gagcggccca taatttttta aagagaattc tgatttaaaa gaagaagaaa atcagcccga
    23941 caatgagaac aatatcggca agcaatgggc aaaaaatccc ttgccaaaga accttctgcg
    24001 gacgaaagcc cacgccgtga atcacaccag cacagacggc ccacatcagc aataaaccat
    24061 gccagatttc cagttcactg gtcttcgcgg caaaacgaga tgggtcccaa aacatacatc
    24121 ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg tccattaccg
    24181 catacaaggt cgcgataatc ttactcatca gatgtcttct tgaccggctt tgcctgattc
    24241 gacgtgttca agcgccagcg cggtgattac cccaaacgaa caggcaagaa gcgttcccag
    24301 aatccatgcg aaataccaca tttttagctc cttacttagt acagagagtg ggtgttacgt
    24361 tcaatatctt ctttggtgat acgaccgaac attttccagt aacaccaggc ggtgtagagc
    24421 agaatgatcg gtaccagaac caccgcaacc caggtcatga cgttaagcgt cagctggctg
    24481 gaagttgcat cccacattgt cagacttgcg ttcatcatgg tgctggacgg catcacaaac
    24541 gggaacattg cgataccggc tgtcaggatg atgcaggcca gcgtcaggga ggagaacaca
    24601 aacgcccacg cggctttatc catacgtgca gtcaggatgg tcagcagcag cagaaccaca
    24661 cccagtgccg gaatagccca cagaattggc gtgttgttga agttaaccag ccatgcgcca
    24721 gcttcacgaa ccacttcttt attcagtggg ttagaggctg cgtaatggtc cattgtcgat
    24781 ttcacgacat aaccatcgat accgtacatc acccatacgc cagccagtgc gaaacagacc
    24841 agtgtcacca gcgcagccac ctgagccgtt gcacgggtac gcaggtgcag ttcgcccacg
    24901 gtacgcattt gcagataggt tgcgccctga gtaatgatca tccctacgct caccacgcct
    24961 gccagcaggc cgaacgggtt aagcaactgg aagaagttac cggtgtagta cagacgcaga
    25021 tattcatcaa cgttgaacgg tacgccctgc aacaggttac cgaacgctac accaattacc
    25081 agcggcggaa cgaagctacc aatgaagatg ccccagtccc acatgttacg ccagcgggtt
    25141 tcttcaatct tggagcggta gtcaaaaccg accggacgga agaacaaaga cgccagcacg
    25201 aggatcatcg ccacatagaa gccggagaac gcagcggcat agaccatcgg ccaggcagca
    25261 aagagtgcgc cgcccgcggt gatcagccaa acctggttac cgtcccagtg tggtgcaatg
    25321 gagttaatca taattcgacg ctcggtgtcg ttacgaccga ggaaacgggt gagcatgccc
    25381 acccccatgt cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc aaccagcagc
    25441 caccagataa aacgcaatac ttcataatcg atcatttgac gactcctgtc ttagcgtgcc
    25501 ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac cggttttcag gctgcttggg
    25561 ccgaggcgtg caaacttgaa cattaagaac aattctgcca ccaggaacag ggtatacagg
    25621 ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg tcagtgacga attcgccaca
    25681 gctgtcggca gcacttcacc gatagcccac ggttggcggc catattcagc cacgaaccag
    25741 cccgcttcta cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg cagaagccat
    25801 tttttctcgc caatgcggtt gcggatgaca ctccagaaag agagcgcgat gattgccagc
    25861 agcaggaagc cacacgccac catgatacgg aacgcaaagt acagcggcgc tacacgcggg
    25921 atggagtctt tggttgcctg ttgaatctgc gcttcagtcg catcagccac gtttggcgta
    25981 tagcgtttca gcagcagacc gtaaccgagg tctttcttca tgctattgaa ctggtcacga
    26041 accgtctggt cggtagaacc agaacgcagt tgttcgagca gagagtacgc cttcatcccg
    26101 ttacgaatgc gttcttcatg ctgcaccatc agctctttca ggccgataac cggggtatcc
    26161 acggaacgcg ttgcaatgat gcccagtgcg taagggatct gaatcgcaaa tttgttcgtc
    26221 tcctcttcct gatcaggaat gccgaacaaa gtaaaggcag caggcgcagg ttgcgtttcc
    26281 cactcggctt caatagcagc cagtttggtt ttctgcacgt cgcccatttc gtagccggat
    26341 tcatcaccca gaacaataac agacagaaca gcagccatac cgaagctggc agcgatagca
    26401 aaggagcgtt tagcgaaggc gaagtcacga cctttcagca tataccatgc gctgataccg
    26461 aggatgaaca tcgcgccagt cacataacca gacgctacag tgtgaacgaa tttcacctga
    26521 gcaaccgggt taagcaccag ctcggagaag ctcaccatct ccatacgcat agtttcaaag
    26581 ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg caaccagaat ccacagtgcg
    26641 gacaggtttg aaccgagcgc caccagccag gtgacacaca tatgctgaac tttacccaga
    26701 cgatcccaac cgaagaagaa cagacctaca aaggtggatt cgaggaagaa ggccatcaga
    26761 ccttcgattg ccagcggcgc accgaagata tcccctacat agtgggaata gtaagaccag
    26821 ttagtcccga actggaactc catggtcaga ccggtagcca cacccagagc gaagttgata
    26881 ccaaacaact tgccccagaa cttggtcata tctttataaa tctgtttgcc ggagaggacg
    26941 tagaccgttt ccataatggc caggaggaac gccataccga gcgtcagtgg cacaaaaagg
    27001 aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat atctaacatc
    27061 atgactcctt gctcatcgca tgaagactcc gagagtgaac cccgttagaa agggtcacac
    27121 gcatgcccca atagaaattt gtttgaacac tttcatcata ccatgtgaat gcacacaaaa
    27181 acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc aaattgatcc cacgtatata
    27241 ttacgccgca aaatccttac aataaacagg tttttgttga agcaaatttg catttttcga
    27301 cggtgatcaa tttatagcta aattgccgcc tttcagccaa tttgatcgag aacaatttat
    27361 ct
//
LOCUS       DAZXUK010000025        27024 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZXUK010000025 DAZXUK010000000
VERSION     DAZXUK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27024
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.025"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..817)
                     /gene="wcaD"
                     /locus_tag="ACH3L8_000894"
     CDS             complement(<1..817)
                     /gene="wcaD"
                     /locus_tag="ACH3L8_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HFT4084121.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYILIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(792..2009)
                     /gene="wcaC"
                     /locus_tag="ACH3L8_000895"
     CDS             complement(792..2009)
                     /gene="wcaC"
                     /locus_tag="ACH3L8_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFT4084122.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2006..2494)
                     /gene="wcaB"
                     /locus_tag="ACH3L8_000896"
     CDS             complement(2006..2494)
                     /gene="wcaB"
                     /locus_tag="ACH3L8_000896"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFT4084123.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2497..3336)
                     /gene="wcaA"
                     /locus_tag="ACH3L8_000897"
                     /pseudo
     CDS             complement(2497..3336)
                     /gene="wcaA"
                     /locus_tag="ACH3L8_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3429..5591)
                     /gene="wzc"
                     /locus_tag="ACH3L8_000898"
     CDS             complement(3429..5591)
                     /gene="wzc"
                     /locus_tag="ACH3L8_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFT4084124.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5594..6037)
                     /gene="wzb"
                     /locus_tag="ACH3L8_000899"
     CDS             complement(5594..6037)
                     /gene="wzb"
                     /locus_tag="ACH3L8_000899"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFT4084125.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6043..7182)
                     /gene="wza"
                     /locus_tag="ACH3L8_000900"
     CDS             complement(6043..7182)
                     /gene="wza"
                     /locus_tag="ACH3L8_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFT4084126.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7491..7640
                     /locus_tag="ACH3L8_000901"
     CDS             7491..7640
                     /locus_tag="ACH3L8_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084127.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7841..9424
                     /locus_tag="ACH3L8_000902"
     CDS             7841..9424
                     /locus_tag="ACH3L8_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFT4084128.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9876..11729)
                     /gene="asmA"
                     /locus_tag="ACH3L8_000903"
     CDS             complement(9876..11729)
                     /gene="asmA"
                     /locus_tag="ACH3L8_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFT4084129.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11751..12332)
                     /gene="dcd"
                     /locus_tag="ACH3L8_000904"
     CDS             complement(11751..12332)
                     /gene="dcd"
                     /locus_tag="ACH3L8_000904"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFT4084130.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12424..13065)
                     /gene="udk"
                     /locus_tag="ACH3L8_000905"
     CDS             complement(12424..13065)
                     /gene="udk"
                     /locus_tag="ACH3L8_000905"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFT4084131.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13383..13625
                     /locus_tag="ACH3L8_000906"
                     /pseudo
     CDS             13383..13625
                     /locus_tag="ACH3L8_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13624..14565
                     /gene="mdtA"
                     /locus_tag="ACH3L8_000907"
                     /pseudo
     CDS             13624..14565
                     /gene="mdtA"
                     /locus_tag="ACH3L8_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14565..17687
                     /gene="mdtB"
                     /locus_tag="ACH3L8_000908"
                     /pseudo
     CDS             14565..17687
                     /gene="mdtB"
                     /locus_tag="ACH3L8_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17688..20753
                     /gene="mdtC"
                     /locus_tag="ACH3L8_000909"
     CDS             17688..20753
                     /gene="mdtC"
                     /locus_tag="ACH3L8_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFT4084132.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20754..22169
                     /locus_tag="ACH3L8_000910"
     CDS             20754..22169
                     /locus_tag="ACH3L8_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4084133.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22166..23569
                     /gene="baeS"
                     /locus_tag="ACH3L8_000911"
     CDS             22166..23569
                     /gene="baeS"
                     /locus_tag="ACH3L8_000911"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFT4084134.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPSPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23566..24288
                     /gene="baeR"
                     /locus_tag="ACH3L8_000912"
     CDS             23566..24288
                     /gene="baeR"
                     /locus_tag="ACH3L8_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFT4084135.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24479..24811
                     /locus_tag="ACH3L8_000913"
     CDS             24479..24811
                     /locus_tag="ACH3L8_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFT4084136.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24958..26319
                     /gene="yegQ"
                     /locus_tag="ACH3L8_000914"
     CDS             24958..26319
                     /gene="yegQ"
                     /locus_tag="ACH3L8_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFT4084137.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26661..26978)
                     /gene="yegR"
                     /locus_tag="ACH3L8_000915"
     CDS             complement(26661..26978)
                     /gene="yegR"
                     /locus_tag="ACH3L8_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFT4084138.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         6151 a         7235 c         7103 g         6535 t
ORIGIN      
        1 tacctaaatc gcccagacgg gtggaaatat aaggaaacgc gattaccaca ccaacccgga
       61 atacagccag agaaatcaat gctaacggta attttttctt aatcgcctct ttattcagat
      121 attgaaatgc ccactccagc aaataaaaca ggataaaggt cataacccct gaaaacgatc
      181 cggataatat tatccctgcg agaatcaggg catctgtttt aggcgttttg ataccaaact
      241 gtttgatgct gagccaaatt gagattaatg ccagagcgaa aaatgccggt tcgaaataaa
      301 gtgctgtcgt gcgcttgccg ccgaatttaa tgaaattcag cacatagctg ttgctgtaaa
      361 tcagatattt cgaaatcgac tccataatac tgctgccacc ggtgaggata atttgcgcca
      421 tctccaccgc cgccagcgcc accaccagtc cgaccaccag ataaaagaaa cgtaatatct
      481 tacgatggtt gtgtggcgaa attgttttaa aacgaatact ccacaccatg ccaataatga
      541 tcaaaatata gacaaacagc atagttgaag tgacgtattt accggcatcc aacgactgac
      601 caaacaggta gttaaacgcc gtgagtcccg cgccaatccc taaggcaatc atcaattttt
      661 taatgctgat gcgttctaaa aacagcagta acaagacagg taaaaaagtg acgatggtga
      721 tggggaaact ttcgccaagc tgggcgattt tgacgttaac cagcaggtag atcagtggca
      781 gcagcaggta gctacagatt ctgatagaag ttgacatact cctccagcat ctgttgtcca
      841 ctgtaggcgg cgcgactgcg ttggctgaac tcagccagcg tggtaccaaa tatcgcctgc
      901 gcgatttccg gtttgcttaa ctgtaccagt tgcagcacct cttcttcact gacggtttta
      961 ccaccggatt tttgcagcac ttcccgcgcc gcatcgctat gggtggcaat caccggcacg
     1021 ccaatcgata gcgcctcaca caaaatcagc gggtagttat cgacacgaga actgaacacc
     1081 agcgcatcca tctgattgag cgcgctcatc aacttgcgct tgtcagtttc aaagccgtga
     1141 ttaaccacgt tgccagcggt gaacggcgag aacttaccaa aggtatgcag ttcgattttg
     1201 tcgcccagcg ccatcatctc acgcaccagt tgctggttag ttttgccgtc gtaacgcagg
     1261 tcatgcgcca ccactgcgat tttcggcttg ccctgagttt cgcgcaccgg aggcagatct
     1321 gccagaatcg cttcggttgc catatcaatg ccattattga taatccggca acgccctggc
     1381 ccgtacagac tattgaaagc gtcagccaca tgctggctgg gggaaataaa ctgacagccc
     1441 agcgccagca tctcgcggaa taactggcgt ttgcccgcca ccagctggtg cgcgcgatca
     1501 atcttcaccg gcggatagtt atttaaggtc gggcatttct ggcagcccgt tttccagcct
     1561 tcgcaaccgt cggtaaaggc gcagcgtccg gttacactcc agtggtcgtg cagcgtccag
     1621 accagggtga catccggttt atggtttttc actttttcgc aaaagcgcac cacgctgtta
     1681 agattcaacc agtagctgtg cagcacatga aaatgcagga ccaccggacc cggtgtgcga
     1741 gtaatggtgc gatataactc attgaaattg ccgaacagat cgcgattaaa cagacgaaac
     1801 aacgcaatgt tcgccatcgc ggtcatccgc ggcgtatgtt tgatgacctg cggatagttc
     1861 tgatggctga cgctctcttt gccgcctttg ccgtagccgt agacaaaatg tgacgccagc
     1921 ccctgttgca gcgcacgctg gtggagatct aacgccacac ctgctgcccc gccttccgcc
     1981 agtcgcacat taaattgcag aatattcatt taatcacctt cactcgcgct ttttctccca
     2041 ccaccagcgc gttgtccggg acagagtcga gcaccacgct gcccgcaccc acggtgacgt
     2101 tgttaccaag cgtgatatca ccaagaataa tgacgttggc accgagttcg acgccgttgc
     2161 caatgtgtgg acatgccatg ttatcggcac cccgattgcc gatagtgacg ccgtggcgaa
     2221 tggtgaaatc atcccccgcc accacgtttt tattgatcac tacggcgtaa ccgtgatgga
     2281 tagtaaagcg gcggccaatg gtcgcggcgg cctggatttc ataaccgaaa aagcattcgg
     2341 tgataatgcg atacagcacc agcagcgggg ccgcccacag attgttgagg acgttctttt
     2401 tgcgccacac cgaacaaaaa tgagcgacgc gataggcaag taccatgcag cacgggcgta
     2461 aactccagct gttggcgcgc agatcttcca gcatctttag cgcccccgaa taccatcagc
     2521 cagacgtttg ccgttacgca ccgacagcag cgtcaacaag gtgcgccagg tcatacgttt
     2581 attgcggatc tggtagaggg taaacagctg atatttttta ctggcgcggt cgaatttgtc
     2641 tttgtgcttg cggtaaaagt ggaagtatcc ggagaatttt ttcggtgacg aggtgatctg
     2701 catttcgccg tgattgatat gcaggatctg cgtcgcctct tccactttcc acggttcgcc
     2761 gtactccacc accatccgca ggaatatgtc gtaatcctgt gctgctttca gttcggtatc
     2821 gaacaaacac tctttgaaac gccatgccca ggtaaagacc tgattgccaa tgatattacg
     2881 tttatagaac aggcggcgtg agtacggcga tttgggatac agtggtaagc tcgccggttg
     2941 ggaataaact tctccctagc aaacgtagtc gttagcgtac aaaaaggcgt gtgtgaccag
     3001 ttgctgttta tgggcgagga agacgctcag gcggttgggc gtccattcat catcatcatc
     3061 gatcccggtg atgtattccc cttgcgccag cataatcgcc tggttacgca ccgcgcacgc
     3121 cccgctgtta atgtcgttgt ggatgtaagt gatacgcgga tcgttgaggg cagtgacgta
     3181 ctgttgcagt tgttcccagg aggtggagca atcatccacg atgatcatct cccagttgct
     3241 gtagtcctgg cgcaggaccg attttatcgc ccgaatcgcc agttgttggc ggttccaggt
     3301 tggcatatag attgagatca gcggattgtc tttcataggt tgttctcccg gcttatgagc
     3361 tggtttgctt gccggatgcg gcgtgagcgc cttatccggc ctacggggcg gtgcgaatgc
     3421 aggcgtcgtt atttcgcatc cgacttatat tcgtattcgt aatagccata atcctgatac
     3481 gcgctggcgc ggcggaagat ggagttcaga atcacccctt tcaccggaat accgttttgc
     3541 tcaaagcggc tcagactggt ttccacttct ttcaatgtgt tgaccgcata acgcgccacc
     3601 attaacgtgg ttccgacatg acgaccaaca attgccgcat cggtcactgc cagaatcggc
     3661 ggcgtatcaa tcaacaccag gtcatagttt ttactcgccc agttcaccag ttcggcaaag
     3721 cgttcgctca ttaacagttc agaaggattt ggcggcacct gaccgcgcgg gatcaggtca
     3781 aatttggcaa tagaggtcgg tttagcagcg gtagtaatat cgccctgacc aatcagaatc
     3841 tccgacagcc cattgacatt attggtgccc aacagctcgt gggtgtagcc tttgcgcata
     3901 tcgcagtcga tcaacaacac gcgtttattg gtctggctga tcaccgccgc caggttggcg
     3961 cagacaaagg ttttaccgat tgacgggcta accccggtca tcatcaacac attgttctgc
     4021 gcctgcatca tcgcgaagtg cagactggta cgcagactac ggatggcttc aatcgccaga
     4081 tcggttggat tccccactgc cagtagctgg ctctgtttat agcgtttaac ccctttgatg
     4141 gttttgacgc tatcgcgcgc tttctgccac tccgacagcg ggatgctggc atagacgctg
     4201 attccgtgtt cttccagcac ttgcgggctt tcgatgccgc gattaaacaa cgagcgcagc
     4261 agcacaccca cgatagagag catcaggcca aggataatcg cgccgaggat aatcagccct
     4321 ttcttcggtt ttagcacgcc aggctgggtg attgccgggt caataatgcg cacatcgccg
     4381 acggtgctgg cttcggtgat tttcagctcc tgctctttat tcagcagttg catatagacc
     4441 tgctgaccag actcgacatc gcgggtcaga cggacaattt cctgctggtt tttcggcatc
     4501 gccgttacgc gaccgttaag tttggctttc tcttcttcca gcgcctgacg tttctccagc
     4561 agcgtgcggt acgccgggtg aactttggtg tacagcttgg agatttccgc ctctttaaag
     4621 gtcagttcgt tcaactgcgc gtcgatgttc accatcgaat cgagcaccgc tttcgcttca
     4681 agcggcagat caacagaatc tttatcctga cggaaggcat tcagtttgtt ttcggcaaca
     4741 tcgaggcggc tacgtacttc cggtaactgt tgcgcgagga aagcgaggct tttcgacgct
     4801 tccgccgatt tgcgctcaat attttgttcc tgatagttac gagcgatgct gttaagaatg
     4861 tcgcggatct gttcgcgatc ttcaccggta taagtcaggc tcagtacgcc tgcgtctttg
     4921 ccgttctccg ttaccgtcag gctgttttgc agttggttga tcatccccaa cgtggagtat
     4981 ttggtgacgg taaactcact gcccgggcgg gcgtgaatgg cttcaaccat cagcgtgacg
     5041 ccttcttttt tcagcatctg gcccgcttgc ccacgggcgc taaagccgcc atcactgctc
     5101 agggtgtagt ttttgtcatc cagcacatta agcgtaaaca cctgatccgc catctctttc
     5161 gggcggttaa aggtagtcac tttcaccgtc tcgttctgac gccccatcag gcgatcccag
     5221 cccgccccga aaatcgggaa cgtgtttttg ctcaccgcaa tatcgaggtc gagatcgtcc
     5281 accgttttac caagcaccag acgcgaacga atcaactgga tctcggcgtc cgatgcaggc
     5341 ggtttgttgg ctaatgccga gccgatatcc tgcaccagcg aattgccgct gttttgctcg
     5401 atttgtacca gtgcgtcggc actataaatc ggcgtggcga agaaagtgta aaccacggca
     5461 cagagggcaa atacgacggt gatgccaatc acccaccagc gcgcttcaat gacggtgccg
     5521 accaggcgac caatatcgat ttcatcactg cccgttaccg gagcggcatg ttgttttact
     5581 ttttctgtca ttcttatacc tgctctgcgt tcaatgcctg cgcccactgg cgggcagacc
     5641 gttcaagtaa tgtgtacacc gctgcaaacg tttcccggct tttgtgatac ggatcgggga
     5701 tttcacattc gttatcccag tgaccaaaca gcatcacttt gccgcgcatc tccggtgcca
     5761 tctcgcataa gcgttcgata tggcgcttat ccatggtcaa aatcaggtca tagttgcgac
     5821 acaggcggcg gctgatttgt cgtgcacagt ggccttccag agacagttga tgttctgcgg
     5881 cgacgctgat agcggtagga tcagcgccct taccgaccag cgcgccgagt ccagcggact
     5941 ccactttcag ttccgggtga taacgttgca gtaagcgttc cgccatcggg gaacggcaaa
     6001 tattgccgac acagacaact aagatgttgt taaacatgac gattaccagt tatgaatgtc
     6061 gctggctgta tccgtcatgt aacggacacc gctaatagtt ggcagcaact gattgatcag
     6121 acggttccag cgggaaaccg gggcggtggt gacatacacc acgtcataag gttgcaggcg
     6181 gaattctgtc gccatcacca gcgacgtcgc atcggacata tccagctggt agatattggc
     6241 aatcttgccg ttacgcccgc cctcgccttt cagcggacga atgacaaaga tgccgctggc
     6301 gttggaggtg gtcatgtcga taccttcggc attggccaga gcttcagtca gggtcatgcc
     6361 gctaaagtcc attttcaggg tgctctgttt cttcacttca cccatcacaa atactttcag
     6421 atcatcatta cgcggcacgt agagaatatc gccgggataa agcagtcggt tctgattgag
     6481 gtcgccgttt tgcatcagcg cctgtaaaga aatgcgttct tcacgaccat tgtgtgttag
     6541 caccacgttg cgccagtcag cggtgtcggt caggccacct gcggcgttga tggcgtcgag
     6601 aatggtcagt ggtacgttgg tgatcgcctg ttgaccggat ttattcacct gacctgagat
     6661 ataggccttt tgtgagcgga aggcggcgat attaacgtcc acctgcgggt cagcgatgta
     6721 cgtcgctaag cgcccggtaa tatcactgcg gatttcagcg agcgttttcc cgactacgtg
     6781 aaccttgccg atatacgggt aaaacatagt gccgtcaggc tgtacccagt tgccggtgtc
     6841 gctggagctg cggtactgac cagctggcgt ggtgagttcc gggtgatccc agacggtgac
     6901 attaagaacg tcccccggcc cgacgcgata ctggtaattc gcgatctcgc tttccagcgt
     6961 catattgggg cgcgctacat tcgggcgtgg gcgtaattgg tcaatcaggc gcggggtcag
     7021 cggataaaca ttcaccattt tgtcgagatc gaaatcagcg tcctgctgtt tgatgacgtc
     7081 tttgcccatc gtcgacatat tgctgcccgg aagtactgtg caaccgctta tcaaggttac
     7141 tgacaccaat aatggcatca atttcatttt ggatttcatc attgtttatt tatcactttg
     7201 gcagagtaat tatcctgtgc actattaata gcaatgtcgc catgcacatt taccttgcag
     7261 ttaattgaat aaaaatttaa ctggcatcag tcctaaaaaa attgatttca tccgcaggct
     7321 attgacagaa taattcagac tggtctttca ggcatccaga cacgctaccg cccctggctt
     7381 tttagctacc aatacactga ttgagcttaa tttttcaaac cctctctgca tacaatcgtt
     7441 gatgagaaag ggttattgcg gaaattaact tccgaatata aggtgacatt atggtaattg
     7501 aatattggct cgccaataat gcaggaggaa gtgttacagc taacggaata gcaggcaagg
     7561 taacaattcg gcaattggct atttttaaga attatattaa gtatcattca atatggtttt
     7621 taggagtttc tttaggttga caatatttaa tattgcgtct ccacatgcga tatttcttaa
     7681 ataatgtttt attattaccg cttttaattc agggataatg tgaggttatt accctcaaat
     7741 aatatgagat aaatagtgct gcaacattgc atttttgccc cgatttatcc atgatcgaat
     7801 tgtgacattt gtcatacaac gaataggttt tgtacttact atggaatgga ttgccgatcc
     7861 gtctatctgg gccgggttaa tcacgctgat tgtgatcgaa ctggtcctcg gcattgataa
     7921 cctggtcttt attgccatcc tcgccgaaaa actaccgccg aagcagcgcg accgcgcacg
     7981 ggttaccggg ctgctgctgg cgatgttaat gcgcctgtta ctgctagcgt caatctcctg
     8041 gctggtcacc ctgactcaac cgctgttcag cttccgctcg tttaccttta gcgcccgcga
     8101 cttaatcatg ctgtttggtg gtttcttcct gttgttcaaa gccacgatgg agctgaacga
     8161 acggctggaa gggaaagaca gcaataatcc cacacaacgc aaaggtgcga agttctgggg
     8221 cgtggtgacg caaattgtgg tactggacgc cattttctca ctcgactcag tgattaccgc
     8281 cgtcgggatg gtcgaccatt tactggtcat gatggccgcc gtggttatcg ctatcagcct
     8341 gatgttgatg gccagcaagc cgttaacgca atttgttaac agtcacccga ccatcgttat
     8401 tctctgctta agcttcctgt tgatgattgg ctttagcctg gtggcagaag gtttcggctt
     8461 cgtcattccg aaaggctacc tgtacgctgc cattggtttc tcggtgatga tcgaggcact
     8521 caatcagttg gctatcttta accggcgacg ttttctttcc gctaatcaga cgctacgcca
     8581 gcggactacc gaagcggtaa tgcgcctgct tagcgggcaa aaagaagatg cggaactgga
     8641 cgccaaaacc gcgtccatgc tggtggatca tggaaaccag cagatcttta atccgcagga
     8701 acggcggatg attgagcggg tacttaatct taaccagcgt accgtaagca gcattatgac
     8761 gtcgcgccac gatattgagc atatcgatct caacgcgccg gaagaagaaa tccgccagtt
     8821 gctggagcga aatcagcata cgcggctggt ggttaccgac ggtgatgacg cagaagattt
     8881 gctcggtgtt gttcacgtta tcgacctgct acaacagtca ctgcgcggcg aaccgctcaa
     8941 cctgcgggtg ttgattcgcc agccgctggt gttcccggaa accttgccgt tgttaccagc
     9001 cctggagcag ttccgtaatg cccgcacgca ctttgctttt gtggtggatg agtttggctc
     9061 ggtggaaggg attgtgacat taagtgacgt cactgaaacc attgccggta acttaccgaa
     9121 cgaagtggaa gagatcgacg cccgccatga tattcagaag aatgcggacg gttcctggac
     9181 ggcgaatggt catatgccgc tggaggatct ggtgcaatat gtgccgctgc cgctggatga
     9241 aaaacgtgaa tatcacacca ttgccgggct gttgatggag tatttgcagc gtattccaaa
     9301 acctggcgaa gaagttcagg tgggggatta tttgcttaaa acgttgcagg tggaaagcca
     9361 tcgcgtgcag aaggtgcaga ttataccgct gcgtaaggat ggcgagatgg agtacgaggt
     9421 gtgatggtat cggccatttt acccaatgct gcgtaacgcc ttataacttc agcaatgaag
     9481 cgttgtctgt ggtaaacgga catcacagcg ggggtgtggg tccggctgta aatcgccgaa
     9541 tcgtttcctg taggctgggg cagcccacat ggatgtgggc tgagggcgcg ttttacaggg
     9601 atgttacctc gcgcccgtac ccgatagccg aaaggaataa gatgagggaa ccgcgccagc
     9661 ggcgatttac cgccgggagc ccgggttgcc agggtggtgg cgattgagcc accctggcac
     9721 gttcaccggc ttgagcgttc catagtagcg aggaacatca agtgaacgga acagccttcg
     9781 atcttacata ttcagaatat aatagtgccg ccgcgaacgc ctgatgcgac gctttacgcg
     9841 tcttatcagg cctacaggac aggtcacctg gtaacctaca tcttctccag caacttcttc
     9901 acatctttgc cattgcggga atctttattc cgctcggccc agtcattcag gcgacgtttc
     9961 gcttcgtcct gtagatgttt gcgcagtaac tgatccactt gcaggctgta attgagttgc
    10021 tgccagttgc cataaacccg cagcggtact ggcgtttctt tgaggaaatc aatcagtttg
    10081 ctttccccgt tccagccacc cacgacccga atatcaaact gggtgtcgca ggtttgatct
    10141 gccagattca acatgccttc ccctgtcagc gccagcacag gcgattgacc ttgcatgtcg
    10201 tttaacgtca cgacgccatc cttcaacgtc aaatcggtgg taaagcggtc aagccgcgtc
    10261 acgttatcga agttttcagc ggccttcaca tcaccgccat tacgctctac cgcttgctga
    10321 atcatctgct ggaagttcat cccttccata cgtgtgtcgg tcatctcaac atgcgcctgt
    10381 ccttgccagt tgtggcggaa tgcgtcggca tctatgtcag caccggagaa atcaccagcc
    10441 agtgacattt ttccggtcaa cgaaatcgga tagttaaacg ccttcagaat ggtaccaatc
    10501 tcaacgttct ccagccgtgg ctggaagttt atccgcggat ttattgatgt cgcgtccagc
    10561 gtgcccggca gtgaaacctt tccaccgtta agcttgccct gcagttgagt aatttccagc
    10621 aaacccgact tgttggtcat ttgcgtggca acatctgtaa aattcattcc gcgccagcgc
    10681 acgttactgg cctgcaacaa aatatcagct gtaaagcctt gcagtccctg ataggccggt
    10741 tcatcaatac gcgaagaaat gaccgggcgc ggcaacgttg attggctctg cccctgctgc
    10801 gcggcaccgt tttcaccatt cgcggtttca ttaagcggga tgaggttgtc aagattcagt
    10861 tgcgggaatt gcagtctcag ctgccattcc ggtttctctg tcagcgtgac ctgtgcttgt
    10921 ccgctcagcg tactgtcatt ggcggtcaaa ctaatttggt taaatgaaag gcgtttatgt
    10981 gactcctgcc actgggactg gaaactcccc tgcccctgaa taccttgttt tggtaaatcg
    11041 gcaccctgca actgccagtt aatttgttca atagccgctg ttaaatcatg cggataatca
    11101 gaagcatcta ccgtaccgtt aagggatatc gtgagatcgc gctgatcgcg attaacccgc
    11161 ccggagaact caaatgagcc acgatgttgg ggatcttgtt ccatttgcag gcggatattg
    11221 cggattgtca cctgctcgtc atcttcatgc tggaacacca gaacgctgtc cgccacctta
    11281 agactggata tatcaaacga ccatccgcga tcgtctgaca gatccggcaa ggtattgtcg
    11341 cgcggtgcaa ccggagcgtc ttcactgcgc accgcttccg tctgcggcgt cagttggatc
    11401 actgcccctt ttagcatcac ctgcttaacg ctcagttgat gactcagtag tggtaaaagc
    11461 gccacgtcca gacgcatgtt gtcggcgcga accagtggct ggcttgcgcc ctgggcggtg
    11521 agagacattc gcccggagag gatactaagc tgcggccaga cgtgccaacg cagtggcccg
    11581 tcgagctgca attgataacc gctacgtgca gcaacttgct tgaccatata gtcgcggaaa
    11641 tcattcggat tcaccagcaa cactaacgca gataacccgg ccaccagcac gaccaagagt
    11701 atcatcagcg tcgtcagaaa tcgtctcatg gtatcctcaa tgggcctgaa ttagtcttta
    11761 tcgattcggc tggctaccgc gccctgctgg ttgcgatatt tcgcatcttc acggcggttg
    11821 taaggtcgcg ccgccgggcc ggaaagcggc tcaaagctca gcgcaccaat taacatgccc
    11881 ggacgcagcg ccagcggcag cttaccggag ttgtagaact ccagcacaat gcaaccagac
    11941 cagcccggat cgatacggtg cgcggtgacg tgcaccatca gccccagacg cgccaaagag
    12001 gaacgcccgt ccagccagcc caccagatcg gctggcagcg tcaccgactc cagtgtcacc
    12061 gccagcgcca gctctcctgg gtgaagatag aacgcctcgc cctcgtcgag aacgatctca
    12121 tcgctcatca cgcggtcaag cgcggcgctc acttcatctt tgggaccgct cagatcgata
    12181 aacgctgccg tgtgaccacg gaaggtacga aatttattgc ccaggcgtac atccaccgtc
    12241 gcgccgttaa tacgctccac tggcggacgt gggttgatcg acaaacggcc ttcatcaagc
    12301 caggcttcaa tatctcggtc acacagacgc atggcatttt ctcctttcgc acatcactcc
    12361 cttaacgcca attacgtcaa gggcatacca acttatcact gaacggtaca caatttatca
    12421 agcttattca aagaactgac ttattttcgc tttcaatata tcgatcgcga tgcggttttt
    12481 cccgccgcgc ggcacgataa tgtccgcata ttgtttagaa ggctcaatga attgcaggaa
    12541 catcgggcgc acggtttttt gatattgcgc catcacggaa tccattgaac gcccacgctc
    12601 gttaacgtca cgcttgatgc ggcgcatcag gcagatatcc agcggggtat caacgaaaat
    12661 ggagaagtta agttcgtcac gcaaacgcgc atccgtcagc aacaaaatgc cttcgagaat
    12721 gatgaccttc ttcggctcaa ccgtcaccgt ttctttcata cgcgtatgtt caacatagct
    12781 gtaaaccggc aggtcaattg ccgagccgcg tttcaacgct tgtaaatgct caagcaacag
    12841 gctgtgatcc atcgcgctgg gatggtcgta gttggtctta acgcgttctt ccatcgacag
    12901 atggctttga tctttgtaat agcagtcttc gggaattacg ccgatgtgtt catcaccgac
    12961 ttgctcacgt aattcacgat aaagggtact ggcaataaga ctcttgccgg aagccgatgc
    13021 gccagcgata ccgataatga cgcactgatg agactgatca gtcatatatt tagcgacctg
    13081 attaacctgg atgttaggaa gggggcgacg aagcgccaaa cgcggcaatt atagggattt
    13141 catccgtctg ataccagtcg aatagcgttg ccgcgcgctc agagttaatt gttgacaaag
    13201 aattcccggt ggcaaattac gttgatcagt tttatgtaag gtaaaaaatg ttatacgcag
    13261 ttgcgcaaat tatccgcctt tacgtcactt tatgagcaat tcgcatataa aatgtaaaac
    13321 ttttgtacta gcataaacac agaaacgaat actggcgacc tggccttgcg gataaagcgg
    13381 taataagcaa acaatcacag catgtattaa ttgccctgcc ccacccgctg cttcacctgg
    13441 tcagtttagg tttagtctcg tttatcttta cccttgtctc gcttgagctt tcgcagtttg
    13501 gcacccaact cgccccactg tggttcccga cgtccatcat gatggtggcg ttttatcgcc
    13561 atgccgggcg catgtggccg ggaattgcgc tgagctgttc gctgggaaat atcgccgcat
    13621 ccattccagg aaggccagca ggtcaaagca ggcgatttac tggcagaaat tgaccccagc
    13681 cagttcaaag ttgcattagc acaaacccag ggccaactgg caaaagataa agccacgctt
    13741 gctaacgccc gccgtgatct ggcgcgttat cagcaactgg caaaaaccaa tctcgtctcc
    13801 cgtcaggagc tggatgccca acaggcgctg gtcagtgaaa ccgaaggcac cattaaggct
    13861 gatgaagcaa gcgtcgccag cgcgcagttg caactcgact ggagccgcat caccgcacca
    13921 gtcgatggtc gcgttggtct caagcaagtt gatgttggta accaaatctc cagtggtgat
    13981 accaccggaa ttgtggtgat cacccagacg catcctatcg atttagtctt taccctgccg
    14041 gaaagcgata tcgctactgt agtgcaggcg caaaaagccg gaaaaccgct gatggtagaa
    14101 gcctgggatc gcaccaattc gaagaaatta agtgaaggca cgctgttaag cctcgataac
    14161 caaatcgatg ccacaaccgg tacgattaaa gtgaaagcac gctttaataa tcaggatgat
    14221 gcgctgtttc ccaatcagtt tgttaacgcg cgcatgttag tcgacaccga acaaaacgcc
    14281 gtagtgatcc caacagccgc cctgcaaatg ggcaatgaag gccattttgt ctgggtgctg
    14341 aatagcgaaa acaaggtcag caaacatctg gtgacgccgg gcattcagga cagtcagaaa
    14401 gtggtgatcc gtgcaggtat ttctgcgggc gatcgcgtgg tgacagacgg cattgatcgc
    14461 ctgaccgaag gggcgaaagt ggaagtggtg gaagcccaga gcgccaccac tccggaagag
    14521 aaagccacca gccgcgaata cgcgaaaaaa ggagcacgct cctgatgcag gtgttacccc
    14581 cgagcagcac aggcggcccg tcgcgcctgt ttattatgcg tcctgtcgcc accacgctgc
    14641 tgatggtggc gatcttactc gccgggatta tcggttatcg cgccctgccc gtttcggcgc
    14701 tgccggaagt ggactatccg accattcagg tggtcacgct ctacccaggt gccagcccgg
    14761 atgtcatgac ctctgccgtt accgcgccgc tcgaacgcca gttcgggcag atgtctggcc
    14821 tgaaacagat gtcgtcgcaa agttccggcg gtgcgtcagt tatcactttg cagttccagc
    14881 taacattacc gctcaatgtc gccgagcagg aagtgcaggc cgcgattaac gctgcgacca
    14941 acttgttgcc gagcgatctg cctaacccgc cggtttacag caaagtgaac ccggcagatc
    15001 cgccgatcat gacgctcgcc gtcacctcaa ccgccatgcc gatgacgcaa gtggaagata
    15061 tggtggaaac ccgcgtcgcg cagaaaatct cgcagatttc cggcgtcggc ctggtgacgc
    15121 tttccggcgg tcagcgtccg gctgttcgcg tcaaacttaa cgctcaggcg attgccgccc
    15181 tcggcctgac cagcgaaacc gtgcgcaccg ccattaccgg cgctaacgtt aactcggcaa
    15241 aaggtagcct cgacggccct tcccgtgcgg tcacgctttc cgcgaacgac cagatgcaat
    15301 ccgccgaaga gtatcgccag ctaatcatcg cctaccagaa cggcgcgcca attcgtctgg
    15361 gcgatgtcgc aactgtagag caaggtgcag aaaacagctg gctcggcgcg tgggcgaaca
    15421 aagaacaggc cattgtgatg aatgttcagc gccagcccgg tgctaacatt atctccaccg
    15481 ccgacagcat tcggcagatg ctgccacagc tcactgagag tctgccgaaa tcggtgaagg
    15541 tgacagtgct ttccgatcgc accaccaata tccgcgcatc cgtcgatgat actcagtttg
    15601 aattgatgat ggctatcgcg ctggtagtca tgattatcta cctgtttttg cgcaatattc
    15661 cggcgaccat cattcccggt gttgctgtac cgctgtcgtt aatcggcact ttcgcggtta
    15721 tggtgtttct cgatttttca atcaataacc tgacactgat ggctttaact atcgccaccg
    15781 gattcgtggt cgatgacgcc atcgtggtga tcgaaaacat ttcccgctat atcgaaaaag
    15841 gcgaaaaacc gttggcggcg gcgctcaagg gcgcaggtga aatcggcttt accattatct
    15901 cgctgacctt ctcactgatt gcggtgttga tcccactgct gtttatgggc gatatcgtcg
    15961 ggcgactgtt ccgcgaattt gctattaccc tggcggtagc gattttgatc tcagcggtgg
    16021 tgtcgctgac cctgacaccg atgatgtgcg cgcggatgct cagccaggag tcgttgcgta
    16081 aacagaacag cttctcccgt gcctcggaaa aaatgttcga caggataatc gccgcctatg
    16141 gtcgtggact ggcgaaagtg ctgaatcatc cgtggctgac cttaagcgtg gcactcagca
    16201 cgctgctgct tagcgtgctg ctgtgggtgt tcattccgaa aggtttcttc ccggtacagg
    16261 acaacggcat tattcagggc actttgcagg caccgcaatc cagctccttt gccaatatgg
    16321 cccagcgaca acgccaggtc gcggacgtga ttttgcagga tccggcagtg caaagcctga
    16381 cctcatttgt tggcgttgat ggcactaacc cgtcgctgaa tagtgcacgt ttacaaatca
    16441 acctcaaacc gttggatgaa cgtgatgatc gggtgcaaaa agtcatcgcc cgtctgcaaa
    16501 cggcggtaga taaagtgccg ggcgtcgatc tcttcctgca accaacgcag gacctgacca
    16561 tcgatactca ggtcagccgc acccagtacc agtttacctt gcaggccacg tcactggatg
    16621 cgctcagtac ctgggtgcca cagttgatgg aaaaactcca gcaactgcca cagctttctg
    16681 atgtctccag cgactggcag gacaaagggc tggtggcgta tgtcaatgtt gatcgcgaca
    16741 gcgccagccg tctggggatc agcatggcgg acgtcgataa cgccctgtac aacgcgtttg
    16801 gtcagcgact gatttccact atttatactc aggctaacca gtatcgcgtg gtgctggagc
    16861 acaataccga aaatacccca ggcctcgcgg cgctggatac cattcgcctg accagcagcg
    16921 acggtggcgt ggtgccgtta agctcaattg ccaaaattga gcagcgtttt gcgccgcttt
    16981 ccatcaacca tctggatcag ttcccggtaa caaccatctc ctttaacgtg ccggataact
    17041 attcgctggg cgatgcggtg caggcgatta tggacaccga aaagacgctg aatctgccgg
    17101 tggatatcac cacgcaattc tagggcagca ccctcgcctt ccagtcggcg ctgggcagca
    17161 ctgtctggct gattgtcgcg gcggtggtgg cgatgtatat cgtgctcggc attctgtacg
    17221 agagctttat tcacccgatc accattctct cgacgctacc caccgcaggg gtcggcgcgc
    17281 tgctggcatt aatgattgcc ggtagcgaac tggatgtgat tgcgattatc ggcattattt
    17341 tgctgatcgg tattgtgaag aagaacgcca tcatgatgat cgacttcgcg ctggctgctg
    17401 agcgcgagca aggcatgtcg ccgcgcgatg caatctacca ggcttgtctg ttgcgttttc
    17461 gtccgatcct gatgaccacc ctggcggctc tgcttggcgc gctgccgctg atgttgagta
    17521 ccggggtcgg cgcggaactg cgtcgtccgt taggtatcgg catggtcggc ggtctgattg
    17581 tcagccaggt gctgacgctg ttcaccacgc cggtgattta tctgctgttc gaccgcctgg
    17641 cattgtggac caaaagtcgc tttgctcgcc atgaagagga ggcgtaagtg aagttttttg
    17701 ccctattcat ttaccgcccg gtggcgacga ttttactgtc ggtcgccatt accctgtgcg
    17761 gcatactggg tttccgtatg ctgccggtcg ccccgctgcc gcaggtcgat tttccggtga
    17821 ttatggtcag cgcctcgctg cccggtgcgt cgccggagac aatggcgtct tccgttgcca
    17881 cgccgctgga gcgctcactt gggcgcattg ccggggttag tgaaatgacc tccagcagtt
    17941 cgctcggcag cacgcgcatt attttgcagt ttgattttga ccgggatatc aacggcgcag
    18001 cgcgtgatgt gcaggcggcg atcaacgctg cacaaagttt gctgcccagc gggatgccca
    18061 gccgcccgac ctatcgcaaa gcgaacccgt cggatgcgcc aattatgatc ctcacgctga
    18121 catccgatac ttattcgcag ggtgaactgt acgatttcgc ctcgacgcag ctggctccga
    18181 cgatttcgca aatcgacggt gtcggtgatg tcgatgtcgg tggcagctcg ctgcccgccg
    18241 tgcgcgtcgg gctgaccccg caggcgctgt ttaatcaggg cgtgtcgctg gacgacgtac
    18301 gcactgccat cagcaatgcc aacgtgcgta aaccgcaggg ggcgctggaa gatggcactc
    18361 accgctggca gatccagacc aacgatgaac tgaaaaccgc cgctgaatat cagccgctga
    18421 ttattcacta caacaacggc ggcgcggttc gtctgggcga tgtggcgacg gtgactgact
    18481 cagtgcagga tgtgcgcaac gccgggatga ccaacgccaa accggctatt ttactgatga
    18541 tccgcaaact gccggaagcc aatattatcc agacggtaga cagcatccgg gcaaaattac
    18601 cagagttgca ggagaccatt ccggcggcga ttgatctgca aattgcccag gatcgctccc
    18661 ccaccattcg cgcctcgctg gaagaagtcg agcaaacgct gattatctcg gtggcgctgg
    18721 tgattctggt ggtcttttta ttcctgcgct cgggtcgcgc cactattatt cccgccgttg
    18781 cggtgccggt ttcgctgatt ggtacgtttg cggcgatgta cctgtgcgga ttcagtctca
    18841 ataatctttc gctgatggcg ctcaccattg ctactggttt cgtggtggat gacgccattg
    18901 tggtgctgga aaacattgcc cgtcatctgg aagcggggat gaaaccacta caggccgcgc
    18961 tgcaaggtac tcgcgaagtc ggctttacgg tgctgtcgat gagtctgtca ctggtggcgg
    19021 tgttcctgcc gctgctgttg atgggcggat tgccgggccg actgttacgc gagtttgccg
    19081 tgacgctttc tgtcgccatt ggtatttcgt tgctggtttc cctgacgcta acgccaatga
    19141 tgtgcggctg gatgctaaaa gccagcaagc cgcgtgagca aaagcgactg cgtggttttg
    19201 gccgcatgtt ggtagccctg caacaaggct acggcaagtc gctgaaatgg gtgctcaatc
    19261 atacccgtct ggtgggcgtg gtgctgcttg gcaccattgc gctgaatatc tcgatcccga
    19321 aaaccttctt cccggagcag gacaccggcg tactgatggg tgggatacag gcggatcaga
    19381 gtatttcgtt tcaggcgatg cgcggcaaat tgcaggattt catgaaaatt atccgtgacg
    19441 atccggcagt ggataatgtc accggcttta ccggcggttc acgagttaac agtgggatga
    19501 tgtttatcac cctcaagcca cgcgacgaac gcagcgaaac ggcgcagcaa attatcgacc
    19561 gtctgcgcgt gaagctggcg aaagaaccgg gggcgaatct gttcctgatg gcggtacaag
    19621 atattcgcgt tggtgggcgt cagtcgaacg ccagctacca gtacacgttg ttatccgacg
    19681 acctggcggc actgcgcgaa tgggagccga aaatccgcaa aaaactggcg acgttgccgg
    19741 aactggcgga cgtgaactcc gatcagcagg ataacggcgc ggagatgaat ctggtttacg
    19801 accgcgacac catggcacgg ctgggaatcg acgtacaggc cgccaacagt ctgttaaata
    19861 acgccttcgg tcagcggcaa atctcgacca tttaccagcc gatgaaccag tataaagtgg
    19921 tgatggaagt ggatccgcgc tatacccagg acatcagtgc gctggaaaaa atgttcgtta
    19981 tcaataacga aggcaaagcg atcccgctgt cgtatttcgc taaatggcaa ccggcgaatg
    20041 ccccactatc ggtgaatcat cagggattat cggcggcctc gaccatctcg ttcaacctgc
    20101 cgaccggaaa atcgctctcg gacgccagtg cggcgatcga tcgcgcaatg acccagcttg
    20161 gtgtgccttc gacggtgcgc ggcagttttg ccggcacggc gcaggttttc caggagacga
    20221 tgaactcgca ggtgatcctg attatcgccg ccatcgccac ggtgtatatc gtgctgggta
    20281 tcctttacga gagttacgta catccgctga cgattctctc caccctgccc tcggcgggcg
    20341 ttggagcgct gttggcgctg gagctgttca atgccccgtt cagcctaatc gccctgatag
    20401 ggatcatgct attaatcggc atcgtgaaga aaaacgccat tatgatggtc gattttgcgc
    20461 ttgaagccca acggcacggt aacctgacgc cgcaggaagc tattttccag gcctgtctgc
    20521 tgcgttttcg cccgattatg atgaccaccc tggcggcgct gtttggcgcg ctgccgctgg
    20581 tattgtcggg cggtgacggc tcggagctgc ggcaacccct ggagatcacc attgtcggcg
    20641 gtctggtgat gagccagctc ctgacgctgt ataccacgcc ggtggtgtat ctctttttcg
    20701 accgtctgcg gctgcgtttt tcgcgtaaac ctaaacaaac ggtaaccgag taaatgacag
    20761 atcttcccga cagcacccgc tggcaattat ggattgtggc tttcggcttc tttatgcagt
    20821 cgctggacac caccatcgta aacaccgccc ttccctcaat ggcgcaaagc ctcggggaaa
    20881 gtccgttgca tatgcacatg gtcattgtct cttatgtgct gaccgtggcg gtgatgctgc
    20941 ccgccagcgg ctggctggcg gacaaagtcg gcgtgcgcaa tattttcttt accgccatcg
    21001 tgctgtttac tctcggttca ctgttttgcg cgctttccgg cacgctgaac gaactgttgc
    21061 tggcacgcgc gttacagggc gttggcggtg cgatgatggt gccggtcggc aggttgacgg
    21121 tgatgaaaat cgtaccgcgc gagcaatata tggcggcgat gacctttgtc acgttacccg
    21181 gtcaggtcgg tccgctgctc ggtccggcgc tcggcggtct gctggtagaa tacgcgtcgt
    21241 ggcactggat ctttttgatc aacattccgg tggggattat cggtgcgatc gccacattga
    21301 tgttaatgcc gaactacacc atgcagacgc ggcgctttga tctttccggc tttttattgc
    21361 tggcggttgg catggcggta ttaaccctgg cgctggacgg cagtaaaggt acaggtttat
    21421 cgccgctggc gattgctggc ctggtcgcag ttggcgtggt ggcactggtg ctttatctgc
    21481 tgcacgccag aaataacaac cgcgccctgt tcagtctgaa actgttccgc actcgtacct
    21541 tttcgctggg cctggcgggg agctttgccg gacgtattgg cagtggcatg ttgcccttta
    21601 tgacaccggt tttcctgcaa attggcctcg gtttctcgcc gtttcatgcc ggactgatga
    21661 tgatcccgat ggtgcttggc agcatgggaa tgaagcgaat tgtggtacag gtggtgaatc
    21721 gctttggtta tcgtcgggta ctggtagcga ccacgctggg tctgtcgctg gtcaccctgt
    21781 tgtttatgac taccgctctg ttgggctggt actacgtttt gccgttcgtc ctgtttttac
    21841 aagggatggt caactcgacc cgtttctcct ccatgaacac cctgacgctg aaagatctcc
    21901 cggacaatct ggcgagcagc ggaaacagcc tgctgtcgat gattatgcaa ttgtcgatga
    21961 gtatcggcgt caccatcgcc gggctgttgc tggggctttt tggttcgcag catatcagcg
    22021 tcgacagcgg caccacacaa accgtcttta tgtacacctg gcttagcatg gcgtttatca
    22081 tcgcccttcc ggcgttcatc tttgccagag tgccgaacga tacgcatcaa aatgtagcta
    22141 tttcgcggcg aaaaaggagc gcgcaatgaa gttctggcga cccggtatta ccggcaaact
    22201 gtttctggcg attttcgcca cctgcattgt cttgctgatc agtatgcact gggcggtgcg
    22261 tatcagtttt gagcgtggct ttattgatta catcaaacac ggcaatgaac agcgattaca
    22321 gctgttaagt gatgcgcttg gcgagcagta tgcgcagcat ggtaactggc gcttcctgcg
    22381 caacaacgat cgctttgtct ttcagatcct gcgttcattt gaacacgata attcggaaga
    22441 taaacccagc ccgggtatgc cgccgcacgg ctggcgtacc cagttctggg tggtcgatca
    22501 aaacaacaaa gtactggttg gtccgcgagc gccgattcca cctgacggta cacggcgacc
    22561 cattctggtc aacggtgcgg aagtgggcgc ggtgattgcc tcccctgttg agcggctgac
    22621 ccgcaatact gatatcaatt tcgataaaca acagcggcaa accagctggc tgatcgtcgc
    22681 cctggcaacg ttactcgcgg cgctcgccac ctttctgctg gcgcgcggtt tgctggcacc
    22741 ggtaaaacga cttgtcgatg gcacgcacaa actggcggcg ggcgatttca ctacccgcgt
    22801 aacgcccacc agtgaagatg aactgggcaa actggcgcaa gacttcaacc agctcgccag
    22861 cacgctggag aaaaaccagc aaatgcggcg cgattttatg gccgatattt ctcacgaact
    22921 gcgtacgcca ttagcggtgc tgcgcggtga actggaagcc attcaggatg gcgtgcgcaa
    22981 attcacgccg gagacggtgg cgtctttaca ggcggaggtc ggtacactga ccaaactggt
    23041 tgacgatctt catcagttgt cgatgtctga tgaaggcgct ctcgcctatc aaaaagcacc
    23101 ggtagatttg atcccactgc tggaagtggc gggcggcgca tttcgcgaac gattcgccag
    23161 ccgtggcctg aaactgcaat tttccctgcc agacagtatc accgtatttg gcgatcgcga
    23221 ccgtttaatg cagttattca ataacttact ggaaaacagc ctgcgctaca ctgacagcgg
    23281 cggcagcctg aaaatctctg ccgagcagca cgacaaaacg gtgcgcctga cctttgccga
    23341 cagtgcgcca ggtgtcagtg acgatcagct acaaaaattg tttgaacgtt tttatcgcac
    23401 cgaaggttcc cgcaaccgcg ccagcggcgg ttccgggctg gggctggcga tttgcctgaa
    23461 cattgttgaa gcccataatg gccgcattat tgccgcccat tcgccttttg gcggggtaag
    23521 cattacagta gagttaccgc tggaacggga tttacagaga gaagtatgac cgagttacca
    23581 atcgacgaaa acacaccgcg tattttgatc gtggaagatg aaccgaagct ggggcagttg
    23641 ctcattgatt atctgcgtgc tgcgagctat gcgccgacgc ttatcagcca cggcgatcag
    23701 gtactggcgt atgtgcgcca gacaccaccg gatctgatcc tgttggatct gatgctccct
    23761 ggcaccgatg gcctgacatt atgccgggaa attcgtcgtt tttccgatgt tccgatcgtg
    23821 atggtgacgg caaaaatcga agagatcgac cgcctgctgg ggctggagat tggcgccgat
    23881 gactacatct gcaagccgta cagcccacgg gaagtggtag cgcgtgtcaa aaccattttg
    23941 cgccgttgta aaccgcaacg cgaacttcag caacaggatg ccgaaagccc gttgattatc
    24001 gacgaaggtc gttttcaggc ttcatggcgc ggtaaaatgc ttgacctgac gcctgcggaa
    24061 tttcgtctgc ttaaaacgct ctctcacgaa ccaggaaaag tgttctcccg cgagcaattg
    24121 ctcaatcatc tttatgacga ctaccgcgta gtaaccgacc gcaccattga cagccacatt
    24181 aaaaatctgc gccgcaagct ggaatctctc gacgccgaac agtcatttat ccgcgccgtt
    24241 tacggcgttg gttaccgctg ggaagccgac gcctgccgca tcgtttagtt ttagcgacat
    24301 tactttgtta gccggagatg gcgttcagac ccgatccggc aacattattc cacgcatggt
    24361 cagcaaactg acattatctc tccgccctgg caggcatcag aaaatcgctc atactttaat
    24421 cagtaaacag cacctttaga tgctgttttc gataagcaat ttcaatcaag gagtcattat
    24481 ggctggttgg tttgaactca gtaagagcag tgataatcag ttccggtttg tgctaaaagc
    24541 gggcaatggt gagactatcc tcaccagcga gctgtatacc tcaaaagcct ctgcggaaaa
    24601 gggcatcgcg tcggtgcgta gcaacagccc gcaagaagag cgctatgaga aaaaaacggc
    24661 aagcaacggc aaattctatt tcaatctgaa agccgctaat catcaaatta tcggctccag
    24721 ccagatgtac gccaccgcgc aatctcgtga aaccggaatt gcctccgtta aagccaatgg
    24781 cacaagccag acggtgaaag acaatacgta attacgatgc cgggcgtggg ttgttggcgt
    24841 ccggcgcttt accagatccc ccctacagcg ctacaatgcc cgcccttaaa gtgggggcct
    24901 cccctaaccg cttcatcagg tgaagcggat ctgacctgtc atcagaacga gagaattatg
    24961 tttaaaccgg aactcctttc cccggcggga acgctgaaaa atatgcgtta cgctttcgct
    25021 tatggcgcag atgctgttta tgcgggccag ccgcgttact ccctacgtgt gcgcaacaac
    25081 gaattcaacc acgaaaatct tcagctcggc atcaatgaag cccacgcgct ggggaaaaag
    25141 ttttatgtcg tggtcaacat tgcaccgcac aacgccaagc tgaaaacctt tatccgtgac
    25201 ctgaaaccgg tggtggaaat ggggccggat gcgctgatta tgtccgatcc agggctgatt
    25261 atgctggtgc gtgagcactt ccctgaaatg ccgatccacc tctcggtgca ggctaacgcc
    25321 gtgaactggg cgacggtgaa attctggcag caaatgggac tgacccgcgt gatcctctct
    25381 cgcgagctgt cgctggaaga gattgaagag atccataatc aggtgccgga tatggagatc
    25441 gagatcttcg ttcacggcgc actgtgcatg gcctactccg gtcgctgcct gctctctggc
    25501 tatatcaaca agcgcgaccc gaaccagggc acctgcacca acgcctgccg ctgggagtac
    25561 aacgtccagg aagggaaaga agatgacgtt ggcaacatcg tacacaagta cgagccgatt
    25621 ccggtgcaaa atgttgagcc gacgctgggt atcggcgcgc caaccgacaa agtgtttatg
    25681 atcgaagaag cccagcgtcc gggcgagtat atgaccgcgt ttgaagatga gcacggcact
    25741 tacatcatga actcgaaaga tctgcgcgcc atcgcccatg tagaacgcct gaccaaaatg
    25801 ggcgtgcatt cgctgaaaat cgaaggtcgt accaaatctt tctactattg cgcacgcacc
    25861 gcacaggttt accgcaaagc tatcgatgac gccgctgcgg gaaaaccgtt cgataccagc
    25921 ctgctggaaa ctctggaagg tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt
    25981 catactcacg acgattatca gaactacgaa tacggttatt cagtttctga ccgccagcag
    26041 tttgttggtg agtttaccgg tgagcgcaag ggggacctcg cagcggtagc ggtgaaaaat
    26101 aaattctccg ttggcgacag tcttgagctg atgacgccgc aaggcaacat taactttacc
    26161 cttgagcaca tggaaaacgc caaaggcgaa gctatgccgg tcgcaccagg cgatggttat
    26221 actgtgtggc tcccggtccc gcaggatctt gagctaaatt acgcgctgct aatgcgtaat
    26281 ttctccgggg aaaccacgcg taacccccac ggtaagtgat taatttcgat tatttttccc
    26341 ggatggaaaa ttcttagaaa ccgatcacat acagctgcat ttattaaggt tatcatccgt
    26401 ttcgctgaaa aacataatcc ataaaatgct agctgtacca ggaaccacct ccttagcctg
    26461 tgtaatctcc cttacacggg cttatttttt gtacataagc tgtaaagaag gattgatccc
    26521 tgataaccgt cttccattga ccacatcgat agaatccccc ttcatagcac aatgcctttc
    26581 acgtaacggt atcgtgctcg cccaggttcc ggctacgcac agccagaacg cgcatgtttg
    26641 acgcttacaa aaaaatattc tcactctcca catttgaatg tcagacgagc gacgccatgt
    26701 aatcctgcac cttctgtctt caggtcaact atctgcattt ttttgccctg agtaacacag
    26761 aaatgggctg catcattttt tactatattt tctgcaccag atattctgcc cctggctaaa
    26821 gaagcttcgg cttcggtgta gtattggtta tcgagtttac gctgaatatt acttttatat
    26881 gcaagaccaa atttaccgat acttgtttca tcattatgca cagcacaacc agatataaga
    26941 aaaatactaa ttaatgatat agcagctatc tttttcatct caccttcccc cattaaatac
    27001 caacgacact ctctagtgtt taaa
//
LOCUS       DAZXUK010000026        26476 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZXUK010000026 DAZXUK010000000
VERSION     DAZXUK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26476
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.026"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(50..1240)
                     /gene="bcr"
                     /locus_tag="ACH3L8_000916"
     CDS             complement(50..1240)
                     /gene="bcr"
                     /locus_tag="ACH3L8_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFT4084139.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(1268..1963)
                     /gene="rsuA"
                     /locus_tag="ACH3L8_000917"
     CDS             complement(1268..1963)
                     /gene="rsuA"
                     /locus_tag="ACH3L8_000917"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFT4084140.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            2113..3873
                     /locus_tag="ACH3L8_000918"
     CDS             2113..3873
                     /locus_tag="ACH3L8_000918"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFT4084141.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            3998..4282
                     /gene="rplY"
                     /locus_tag="ACH3L8_000919"
     CDS             3998..4282
                     /gene="rplY"
                     /locus_tag="ACH3L8_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFT4084142.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(4421..5428)
                     /gene="yejK"
                     /locus_tag="ACH3L8_000920"
     CDS             complement(4421..5428)
                     /gene="yejK"
                     /locus_tag="ACH3L8_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFT4084143.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            5610..5837
                     /locus_tag="ACH3L8_000921"
     CDS             5610..5837
                     /locus_tag="ACH3L8_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFT4084144.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            5857..7617
                     /gene="yejM"
                     /locus_tag="ACH3L8_000922"
     CDS             5857..7617
                     /gene="yejM"
                     /locus_tag="ACH3L8_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFT4084145.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            7692..7768
                     /locus_tag="ACH3L8_000923"
     tRNA            7692..7768
                     /locus_tag="ACH3L8_000923"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7726..7728,aa:Pro,seq:ggg)
     gene            complement(7871..10462)
                     /gene="yejO"
                     /locus_tag="ACH3L8_000924"
     CDS             complement(7871..10462)
                     /gene="yejO"
                     /locus_tag="ACH3L8_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFT4084146.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            10782..11429
                     /gene="narP"
                     /locus_tag="ACH3L8_000925"
     CDS             10782..11429
                     /gene="narP"
                     /locus_tag="ACH3L8_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFT4084147.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(11640..12692)
                     /gene="ccmH"
                     /locus_tag="ACH3L8_000926"
     CDS             complement(11640..12692)
                     /gene="ccmH"
                     /locus_tag="ACH3L8_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFT4084148.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12689..13246)
                     /gene="dsbE"
                     /locus_tag="ACH3L8_000927"
     CDS             complement(12689..13246)
                     /gene="dsbE"
                     /locus_tag="ACH3L8_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFT4084149.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(13243..15186)
                     /gene="ccmF"
                     /locus_tag="ACH3L8_000928"
     CDS             complement(13243..15186)
                     /gene="ccmF"
                     /locus_tag="ACH3L8_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFT4084150.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(15183..15662)
                     /gene="ccmE"
                     /locus_tag="ACH3L8_000929"
     CDS             complement(15183..15662)
                     /gene="ccmE"
                     /locus_tag="ACH3L8_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFT4084151.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(15659..15868)
                     /gene="ccmD"
                     /locus_tag="ACH3L8_000930"
     CDS             complement(15659..15868)
                     /gene="ccmD"
                     /locus_tag="ACH3L8_000930"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFT4084152.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(15865..16602)
                     /gene="ccmC"
                     /locus_tag="ACH3L8_000931"
     CDS             complement(15865..16602)
                     /gene="ccmC"
                     /locus_tag="ACH3L8_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFT4084153.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(16644..17306)
                     /gene="ccmB"
                     /locus_tag="ACH3L8_000932"
     CDS             complement(16644..17306)
                     /gene="ccmB"
                     /locus_tag="ACH3L8_000932"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFT4084154.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(17303..17926)
                     /gene="ccmA"
                     /locus_tag="ACH3L8_000933"
     CDS             complement(17303..17926)
                     /gene="ccmA"
                     /locus_tag="ACH3L8_000933"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFT4084155.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(17939..18541)
                     /gene="napC"
                     /locus_tag="ACH3L8_000934"
     CDS             complement(17939..18541)
                     /gene="napC"
                     /locus_tag="ACH3L8_000934"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFT4084156.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(18551..19000)
                     /gene="napB"
                     /locus_tag="ACH3L8_000935"
     CDS             complement(18551..19000)
                     /gene="napB"
                     /locus_tag="ACH3L8_000935"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFT4084157.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(18997..19860)
                     /gene="napH"
                     /locus_tag="ACH3L8_000936"
     CDS             complement(18997..19860)
                     /gene="napH"
                     /locus_tag="ACH3L8_000936"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFT4084158.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(19847..20542)
                     /gene="napG"
                     /locus_tag="ACH3L8_000937"
     CDS             complement(19847..20542)
                     /gene="napG"
                     /locus_tag="ACH3L8_000937"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFT4084159.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(20549..23035)
                     /gene="napA"
                     /locus_tag="ACH3L8_000938"
     CDS             complement(20549..23035)
                     /gene="napA"
                     /locus_tag="ACH3L8_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFT4084160.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(23032..23295)
                     /gene="napD"
                     /locus_tag="ACH3L8_000939"
     CDS             complement(23032..23295)
                     /gene="napD"
                     /locus_tag="ACH3L8_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFT4084161.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(23285..23779)
                     /gene="napF"
                     /locus_tag="ACH3L8_000940"
     CDS             complement(23285..23779)
                     /gene="napF"
                     /locus_tag="ACH3L8_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFT4084162.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            23888..24052
                     /gene="yojO"
                     /locus_tag="ACH3L8_000941"
     CDS             23888..24052
                     /gene="yojO"
                     /locus_tag="ACH3L8_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFT4084163.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            24166..24675
                     /gene="eco"
                     /locus_tag="ACH3L8_000942"
     CDS             24166..24675
                     /gene="eco"
                     /locus_tag="ACH3L8_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFT4084164.1"
                     /translation="MKKESKKMKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     TGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAEVR"
     gene            complement(24797..26421)
                     /gene="mqo"
                     /locus_tag="ACH3L8_000943"
                     /pseudo
     CDS             complement(24797..26421)
                     /gene="mqo"
                     /locus_tag="ACH3L8_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
BASE COUNT         6491 a         7476 c         6506 g         6003 t
ORIGIN      
        1 gacatgcatc aacaaaggaa gccttttacc ttcctcgttg tgcaatagat caccgttttt
       61 tcggccgact ggcgtacaga cagaagagaa tggagctggt tgcgcagaat gcaattgacc
      121 aaatcatcgg ccatgcagag ttaaaggtcg caagagaaag caatgcgcca acaattgccc
      181 ctatgccaaa acggaaggtt cctgccagcg aagatgccgt tcccgccata tggggaaact
      241 catcaagaat gaccgccatc gcattggatg acaccatcga cacgcagccc acaaacgccg
      301 caacgccaac caccagcgac cagaatccca gccccagcgg cgcactgatg accatccacg
      361 cagccataat aaattgtatc cacaaccccg agcggaacat atttaacgcg ccaatgcggc
      421 ggacgaagcg gctgttaaag atggtcatca cgaacagaaa aacaatgttt agcgcaaagt
      481 aataaccaaa gttttccggc gcgacgtggt taatttcaat ataaacaaac ggtccggcgc
      541 ttaagaatga gaacatcccg gcaaagctga aaccactggc aagcatgtag ctcaggacac
      601 gtttatggcg gaacagcgcc gcaaagttac caatagtggt acgaatgtga aatggctgac
      661 gacgctccgg tggtaaggtt tctttaatca ggaagaaaat cattgccgaa gccagaatcg
      721 ccgctaatgc caggatccag aagatgtaat gccagctcag ccacaccagc acccagccgc
      781 caactatcgg tgccatcagc agtgcaatgg tcgtcaccag catgacaaac gacatcatcc
      841 gcgagaactc ttctttcggg taaatatcac gcatcaaagc gttgataacc acgctggccg
      901 cagccgcagc cagcccgtgg aagaaacgca tcacaatcag ctgatcgatg gtttgtgcca
      961 acgcacacgc caccgcggcg gcggcaaaca ccagcgtccc gccaagaacc accggcttac
     1021 gcccgaagct gtctgccatc ggcccgtaga ttaactgccc caacgcaaag cccagaatat
     1081 aagtactgag ggtcatctgc gtactgcccg ccgatacgcc aaactgcgct gaaattaccg
     1141 gtagcgcggg cagatacata tcaatcgaca gcggcatcaa catggccagc aggccaagga
     1201 taaaaacaat agcaaacgac gaatgctgtc gggtggtcac aacgggctcc tgaaagtcat
     1261 tgaaaagtta gacgacgctg gcaatttctt cttcagttaa cggacgatat tcaccggggg
     1321 ctaaatcagc atccagcgta ataccgccaa tacgttcacg atgcagctca accacgtggt
     1381 tacccacggc ggcgaacatg cgtttcacct gatgataacg cccttcgctg atggtcagac
     1441 gaacctgcgt tggggtaatc acttcaagca ccgccggctt agtgagatct ttttcgttat
     1501 gcagctgcac gcctttagca aattgctctg ccgtatcgtc agctacaggt gattccagtg
     1561 tcaccagata ggtcttctcg caatgatggc gcggagaagt aatgcggtgc gaccactgac
     1621 catcatcagt catcagcacc agaccggtgg tatcgatatc cagacgtcct gcggcgtgca
     1681 gtttccacgc taccggttca tccagaaaat agagcaccgt cgggtgatcc gggtcgtccg
     1741 tggagcaaac atagccctga ggcttattga gcatgaagta acgtggaccg tgttgctgcg
     1801 ccagcgggtt gccatcgtaa gcgacatcat gttcagggag cagtttgaac gctgcattac
     1861 gaacgatttc gccatcgacg gtgacacgat tgccgcggat ttcacgcccg gcaatagcac
     1921 ggctaacgcc gagttgctgt gcgataaatt tatcaagtcg catgtgtgtg attttgcctg
     1981 taaaaaacgg aggtcgggta atgacccgaa aatctgaact gttgtctgcc cagtatagcg
     2041 gtctaataac ttccctcaag ggaaaaagaa ccgtggcata ctattagcag aataatctac
     2101 ctacgcgaaa ccatgatttt tacactccgc ccatatcagc aagaagccgt ggatgccacg
     2161 ctcaaccatt ttcgtcgtca taaaacccct gccgttatcg tgctgcccac cggcgcaggt
     2221 aaaagcctgg tgatagcgga actggcacgg ctggcgcgtg gtcgcgtact ggtgctggca
     2281 cacgttaaag aactggtggc gcaaaaccat gcaaagtatc aggcgctggg gctggaagcc
     2341 gatatttttg ccgccgggct aaagcgcaaa gagagccacg gtaaagtggt atttggcagc
     2401 gttcagtcgg tcgcccgtaa tcttgatgcc tttcagggtg aattttcgct gttgattgtc
     2461 gatgaatgcc accgtattgg tgacgatgaa gagagccagt atcagcaaat cctcactcac
     2521 ctgactaaag tgaatcccca cttacgcctg ctggggctga ctgccacgcc ttttcgattg
     2581 ggcaaaggct ggatctacca gttccattat cacggcatgg tacgcggcga tgagaaagcc
     2641 cttttccgtg actgcattta tgagctgccg ctgcgttata tgattaaaca cggctatctg
     2701 acgccgccag aacgactgga tatgccagta gtgcaatacg atttcagccg cttgcaggca
     2761 cagagtaacg ggctgttcag cgaagctgat ctcaaccgtg agctgaaaaa acagcaacgc
     2821 attacgccac atatcatcag ccagatcatg gagttcgccg aaaagcgcaa aggggtaatg
     2881 attttcgccg ccaccgttga acacgcaaaa gagattgtgg gattactgcc tgccgaagat
     2941 gctgcactga ttactggcga caaccccggc gctgagcgcg atgtgttaat tgaagatttt
     3001 aaagcccagc gttttcgcta tctggtcaac gtcgcagtac tgaccaccgg atttgacgcc
     3061 ccgcacgtcg atcttatcgc cattctgcgc cctaccgaat cagtgagtct ttaccaacaa
     3121 attgtcgggc gcggtctgcg tctcgctccg ggcaagactg attgtttaat tcttgattat
     3181 gcgggtaatc ctcacgatct ctacgcgccg gaagttggta caccgaaagg caaaagtgac
     3241 aacgttccgg tacaggtttt ctgccctgcc tgcggttttg ccaacacctt ttgggggaaa
     3301 acgaccgccg acgggacatt gattgaacac tttggtcgcc gctgtcaggg atggtttgaa
     3361 gatgacgacg gtcatcgcga acagtgtgac ttccgtttcc gttttaaaaa ttgcccgcaa
     3421 tgtaacgcag aaaatgatat tgccgctcgc cgctgccgcg aatgtgacac ggtactggtt
     3481 gatccggatg atatgttaaa agcggcgcta cgactgaaag atgcgctggt attacgctgt
     3541 agcggcatgt ctttgcagca tgggcacgac gagaaaggcg aatggttgaa aatcacctat
     3601 tacgatgaag acggcgcgga tgtgagtgag cgtttccgtc tgcaaacgcc cgcccagcgt
     3661 accgccttcg agcagctttt tatccgcccg catacgcgca caccgggcat ccctctgcgc
     3721 tggatcaccg ccgccgatat cctcgcccag caagccttac tgcgacatcc ggattttgtc
     3781 gtcgcccgca tgaaaggtca gtactggcaa gtgcgtgaaa aagtgttcga ttacgaaggt
     3841 cgttttcgtc gggcgcacga attacgcggt taatggtagt tttgattgat gtataaggcg
     3901 attgagtata gaatctcgcc cgcttttgca tacgcaaagc agatcactta cctgttgctg
     3961 ggtcgcctgt agcaggatta atttaagaga gaaagaaatg tttactatca acgcagaagt
     4021 acgtaaagag cagggtaagg gtgcgagccg ccgcctgcgt gccgctaaca agttcccggc
     4081 aatcatctac ggtggcaaag aagcaccgct ggctatcgag ctggatcacg acaaagtcat
     4141 gaacatgcaa gctaaagctg aattctacag cgaagttcta accatcgttg ttgacggtaa
     4201 agaaatcaaa gttaaagctc aggacgtaca gcgtcacccg tacaaaccga agctgcagca
     4261 catcgacttc gttcgcgctt aattgctgaa taagttgtaa aaaaccccgc tccggcgggg
     4321 ttttttgtat ctgcagatta tgcctgatta cggtattgct attttttgca ggccagataa
     4381 ggcgttcacg ccgcatctgg cattcagaac acaaaaccga ttaattgccg ccagacgtcc
     4441 ggcgctgcaa ttggtcgcgc aaattcggcg gcgtgccttt aatggtcaag gtatcggttg
     4501 ctggatccca gaagatgcgt tcacctaaca gcatcgcatc aaagttaata gtcaagccac
     4561 cgccgctacc ggcaaacttc gtcaactgac gcagtgtgct acggtctgcc gggaagctct
     4621 cttccagttc gtagcctttt tcagccgcaa actccgtgaa gctcacttcg cttacgcctg
     4681 ccagctcttt cgacagcgat tccagttcga tctcttcgcc tgcctgcagc tgctcattac
     4741 agtagctata aacctgctgg cgcacgttct gtcgctctgc tttatccagt tgtgcttcgg
     4801 cagtgaaatc atcaaccgct tgcaacagcc cacggttttg cgctttcgcg ttcaatcctt
     4861 cgctggcccc gaggaaatcc ataaagaaat ccgccacttt gcgccctact cgccctttca
     4921 ggaaagtgag atagcgggtg gactctggat tggtttccca ttcggttaaa tcgatacgcg
     4981 caacaatatc cgcatgattg atatcaagat aatgggttgg gttgatgtcg agattttcgt
     5041 taacgcgcat actgctcagg ttgctcagaa ctgccaccag caaatactcc accgccagat
     5101 aacggtagtg gcaaaacagc acgaatccac cgtcagcaaa aggatatttc gctaattcgt
     5161 cacgcaggcg accggttgcc gcgcggctaa atgccagaaa gtcctcttcg ccctgacgct
     5221 gtaagcgcag cgtctgcgcc agttcactct cttcgctaaa caagccgtaa gctttatttt
     5281 tggcgctata gacccgatgc agttctgcca ccatctcaac gacggtttct gtcggttcca
     5341 gcaatgaatc gcgcaacacc agctcaaggt tctgctcatc acgcttgata agctggtgca
     5401 gagcaatctg gttgatatcc agactcatga taaactctcc tttaagaccg ggcggtattc
     5461 aaccaccgcc agttaagcga cgcaataaaa gatggcggtc attttacaga aggcagcgta
     5521 acgagaatga tttaacgcat aaaaaagcag aaaaaaaacc gttgctacgg taatatgttg
     5581 ccctttcatc aactaactga tttcgattta tgccacaaat ttcccgctac agtaatgaac
     5641 aggttgaaca attgctcgca gagctgctca acgtactgga aaaacataag gctccgaccg
     5701 acctttccct gatggtgtta ggcaacatgg tcactaacct tatcaacacc agcattgccc
     5761 cggcccaacg ccaggcaatt gccaactctt ttgccagcgc cttacagtcc tctatcaacg
     5821 aagacaaagc gcactaaggg aaacagataa caggttatgg taactcatcg tcagcgctac
     5881 cgtgaaaaag tctcccagat ggtcagttgg gggcactggt ttgcactgtt caatattctg
     5941 ctttcgctcg tcattggcag ccgttacctg tttatcgccg actggccgac aacgcttgct
     6001 ggtcgcattt attcctacgt aagcattatc ggccatttca gcttcctagt gttcgccacc
     6061 tacttgctga tcctcttccc gctgaccttt atcgtcggct cccagaggct gatgaggttt
     6121 ttgtccgtca ttctggcgac ggcgggaatg acgctattac tgatcgatag cgaagtcttt
     6181 actcgtttcc atctccatct taatcccatc gtctggcagt tggttatcaa cccagacgaa
     6241 aatgagatgg cgcgcgactg gcagctgatg ttcatcagcg tgccggttat tttattgctt
     6301 gaactggtgt ttgcgacgtg gagctggcaa aagctgcgca gcctgacgcg tcgtcgacgc
     6361 ttcgcgcgcc cgctggccgc attcttattt atcgccttta tcgcctcgca tgtggtgtat
     6421 atctgggccg atgccaactt ctatcgcccg atcaccatgc agcgcgctaa cctgccgctt
     6481 tcgtacccga tgacggcgcg acgtttcctt gagaagcatg gcctgcttga tgcgcaggag
     6541 tatcaacgcc gtcttattga gcaaggtaat ccagacgccg tttccgtaca gtatccgtta
     6601 agcgaactgc gctatcgcga tatgggcacc gggcagaatg tgctgttgat tactgtcgat
     6661 ggcctgaact actcacgctt cgagaagcag atgcctgcgc tggcaggttt tgctgagcaa
     6721 aatatttcgt tcacgcgcca tatgagctcc ggcaacacta cagacaacgg catctttggc
     6781 ctgttctatg gcatctcgcc gagctatatg gacggcattc tgtcgacccg tacgcctgcg
     6841 gcattaatta ctgcgcttaa tcagcaaggc tatcagctgg ggttattctc atcagatggc
     6901 tttaccagcc cgctgtatcg ccaggcattg ttgtcagatt tctcgatgcc gagcgtacgc
     6961 acccaatctg acgatcagac cgccacgcag tggatcaact ggctgggccg ctacgcacaa
     7021 gaagataacc gctggttctc gtgggtttct ttcaatggca ctaacattga cgacagcaat
     7081 cagcaggcat ttgcacggaa atatagccgg gcggcaggca atgtcgatga ccagatcaaa
     7141 cgcgtgctca atgcactgcg tgattctggc aaactggaca atacggtggt gattatcact
     7201 gccggtcggg gtattccact gagcgaagag gaagaaacct ttgactggtc ccacggtcat
     7261 ctgcaggtgc cattagtgat tcactggcca ggcacgccgg cgcagcgtat taatgctctg
     7321 actgatcata ccgatctgat gacgacgctg atgcaacgcc tgctgcatgt cagcacacct
     7381 gccagcgaat attcgcaagg tcaggatttg ttcaaccctc aacgccgtca ttactgggtt
     7441 actgctgcgg ataacgatac gctggcaatt accaccccga aaaagacgct ggtgctgaac
     7501 aataacggta aataccgcac ttacaactta cgtggtgaaa gagtgaaaga tgaaaaacca
     7561 cagttaagtt tgttattgca agtgctgaca gacgagaagc gttttatcgc taactgatta
     7621 attataaatc agttagcgaa atatcttact tgcaatcggt gtggaaaacg gtagtattag
     7681 cagccacgag tcggcacgta gcgcagcctg gtagcgcacc gtcatggggt gtcgggggtc
     7741 ggaggttcaa atcctctcgt gccgaccaaa aatcccaaga aaaaaccaac ccttacggtt
     7801 ggttttttta tatctgcaat taattcgata aacagaccgt gacacatcac agcctgttta
     7861 ttttctgtta tcagaacgtc cagaccacac ccgcctgagt attccacggc gattctaccc
     7921 ctgcgccatt gccatagctg actgacaaat gaccgcttaa cgtcggggta aacgatgacc
     7981 ttatcccagc ctgataaacg ccactggttc cagccacatc attattaaat ttgccatcgt
     8041 cattaacttt cacctggtta gaatcggcgt attcctgacg cacggcggct ttcagccagg
     8101 gttccagcgt cgtaccgttt tgcaggtcca tgtgatagct taccgccgtt cccgcttcag
     8161 cgcgtaatat ccgggtattt cccacatccg cgcgcatgcc gtttgataac gtgtagtcct
     8221 gaccatctgt ggtaaagccg gtaaaggcca gatagggtct aacactccac aatccgtcaa
     8281 cccaacggaa cccgctctca acatgagcac ccgcgccgtt actattgtaa tcgccaaacg
     8341 ctgttgcccc attactcatc ttgccatgga tggtgttggc aaaacggtca actttcacca
     8401 ccccatcaac ataggcaccg ttctgatgct cccaaccggc ataagccccc agggtatagc
     8461 tatcgatatt accttttccg ccgcgatcaa aaccaatatc agaatgagag taaccaaaga
     8521 acaagccgcg aattgtactg ctttcttcac gggagaaacg gctatcgata ccgagcgtca
     8581 ggcccgtcaa tgtttgctca aaaccagctc ccgcatcagt ggtcacgttg ttgcgggtgt
     8641 taattgccga actccacatc accgtatcgt aactaacgcc ttttacgcta ccaagacgct
     8701 cacgcacggt gtccagttct gcatcaaata ccagcggttg tgcggctgcc atgttcagca
     8761 catcagtggt tgaaggggta atttgcgcgc gattctctgc gaggctccag ctatggttgc
     8821 cattatccag caaggtatat tcatacgtac cgatatcaac aacgcctccg gcattgccca
     8881 acgtaaatgc agcatcaccg ccgcccgttg ttaccagtgt aaggctatct cctgctgccg
     8941 ggctggcacc ggtgtccgtc acgaatattt tgaaatcacc tgttgcctga ccggtgacgt
     9001 tgagctgatc gctctgatga ttggccatat cggtacgcat ataaaaattg ccgtttcccg
     9061 acagggtatt ggtggtcaac gtaatgaaat tttccgcact tgccgtcaca gatgaacgag
     9121 tcactggctc agccatcata atcacatttg cgccggacat cgcaaggttg gctaagttga
     9181 ggcgagtgtt ttggttcgtc gtagtgatat cggcaatgat ggtccatgcg ctattttcga
     9241 gcgaaacgtc cgctttgctg gtatccacgg caccatgcgt tctaaaagaa gcgccctcag
     9301 aaaccactac cttacccacc aacatagcgg gtgcataatt tatttgcggc ttcatgacct
     9361 caagaatgcc atcattccct ctgactgaca cgttaaccat tgtgccagcc cagacattag
     9421 cgcggccatt gttgattacc atattttcag agatggcttt tgaggaatac gtgatacttc
     9481 cattctggtc tgatcgaccg agatcatata ccgcatttgc ctgcactttc gtaccagtat
     9541 cctttcccag cgattgcata gtggcatgct catcaaggat agtgtcaatg gcctgcgtgt
     9601 cctccataac aatgagcccg gaaccatcat ccagttcata attttttgac acaccatcct
     9661 ttatactaaa ttggccttta ctgtttgtac cactcacttc cagcgcattc gttgagacaa
     9721 ttaatttccc gcctgctttt ttatcaacgc cagttgcagt cccgccatcc ataacctcca
     9781 gtaagccgcc actatccaca gtagtattat aagcgaagtc attttcttca actcgcaaac
     9841 ttccgccgtt ttccaacaac atattattag caataccatt cttgatatcg aactgaccga
     9901 gacggtttgt tccgaatacc tccatggccc gcgtggttgt tttaatagca ccgcctgttt
     9961 tctgatctac cgcaagcgca aatcccccct ctttaacctc cagtagtcct cccttttcga
    10021 tagcggcaaa aacggccccc ccatcttttt caacgattaa gcgcccgcct ttaatcgtgg
    10081 tatcattcgc tgggccataa acctgtagca caccattatt gagggtagtc tggtttgccg
    10141 cgccacccat ttgaacaatt tgataaccat catttaatac tgagtattct gcggtaccat
    10201 tcatttcaat gtattgatac cctccgttaa tttcagtatt actacttaca ccaaattctt
    10261 ttatatgctg ttctccacca ggattgattt tcgtattatt ggcggtccca taaaccagct
    10321 ggatatcttt ttccaggata acaccatcaa ctgtctcacc atactcaact gttgatgcca
    10381 atgctatggg tgaatataac gctgtagcca ccagaacaga tattgcggaa cgacaaagag
    10441 aaacagaacc agattgatgc attgagcttt catcctatga aattaattgc agttaaaagc
    10501 attgggtaca gaaaataccc atagctccat acccggagtc agtttttaaa aactgtttaa
    10561 agaaatgcac aagtattgtg attgattttt tagttgtttt tcttgatgag aagctgatgc
    10621 aaaattccgt ctttataata aaaatgatgc caaagcgaac gacaaggttg tagttttcac
    10681 tacatgtcca tacataaaat ggggtaacat tcacgcgcct ggtagcgtta ccaacgctac
    10741 gctcaaacat aatgattcta ataaaacctc aggagactac tatgcctgaa gcaacgcctt
    10801 ttcaggtgat gattgtggat gatcatccac ttatgcgacg cggtgttcgt cagttactgg
    10861 agcttgatcc tggctttgaa gtggtcgccg aagcgggcga aggcgcgagc gcgatcgatc
    10921 tggcgaatag actggatatc gacgtgatct tgctggatct caatatgaaa ggtatgagtg
    10981 gcctggatac cctcaatgcc ttgcgcagag atggcgttac cgcgcaaatt attatcctga
    11041 ccgtatccga tgcctccagc gatgtctttg cgctgataga cgcaggcgca gacggttatc
    11101 tgttgaaaga cagcgacccg gaagtattgc tggaagcgat tcgtaccgga gcgaaaggca
    11161 gcaaagtctt tagcgaacgc gtcaatcagt acttacgtga acgtgaaatg tttggcgcgg
    11221 aagaagatcc cttcagcgtg ctgacggagc gcgagctgga tgttctgcac gagctggcac
    11281 aggggctgtc aaataaacag attgcatcgg tgttgaatat ttccgagcag acagtaaaag
    11341 tacatattcg caatctgctg cgtaaactca atgtccgctc acgcgtggcg gccaccattc
    11401 tgttcctgca acaacgcggg gcacaataaa aatagcccga tggatttatc atcgggctga
    11461 gatttatgac aaacgcaaaa ctgcctgatg cgctacgctt atcaggccta cgtggatcga
    11521 tcaatttatt gaatttacac aattttgtag gccggataag gcgttcacgc cgcatccggc
    11581 ataaactaag cgcactttgt catcagtcta gcccgatggc atcaccatcg ggcctctttt
    11641 tatttactct cctgcggcga caaatgttgc atcgcctgcg cgatactacg ttcaatcacc
    11701 gcacggcgag tatcgttggc aggtaagagt ttcaacatca tctcccacgc ggcaacggct
    11761 tcgccaaatc gctgctgctc aaaggcatta aacgcataca tgcttagcac acggatattg
    11821 ctatggtccg ttctcaccag ctgacgtagc agttcaccgc cgaggcggtt gtcgttggga
    11881 tcagatgaac gagtcaacgc ttcagcgtat cccagtgcgg catcgctgtt cttcggatcg
    11941 aggcgatacg cagtagcgta tgcatcggtg gcgatactgg cgttacccag cgccatgcca
    12001 acgcggccca acataatcca gccttctata tctcccggat ttttttgcag ttgagtacgc
    12061 atccccagcg caaggcgcga catctcttct tcgttgagcg gatcggcttt cgggtccagc
    12121 gccctgtcca gcaacgccgg agcctgtgcc gtggcctgct gccagatttt cacctgctga
    12181 taattgccag tctggtagta gctgacgcca gccacaatta acgccaccac aatacccggc
    12241 agataaacaa catatccggc acgcttacct tccggcacgc tttgttcagg aaacgcttcc
    12301 ggcaccacgc gtacccgacg ccgcgagcgg gcgtatatga cccaaccgcc aatgccaata
    12361 gccactactg gcagcaccca cagcagcacg gtcagcggcg ttaacggcgg atcgtaagtg
    12421 acgaagttgc cgtaacgcgc caccatataa tcgacaatct cttttttact tttaccttcc
    12481 tgcatcagtt catacacttt ctgacgcagg tcggtggcaa tcatcgagtt ggaatcggca
    12541 atgctgttgt tctggcattt cgggcagcgc agttcttcag tgagctgacg gaactgttgt
    12601 tcctgtgctt catctttaaa ctgcaacacg tcgatggtcg ccagcgctga gccggagatc
    12661 atcagcatca gcacgcccaa taaaaacctc attgtgcggc ctccttactg tatttctccc
    12721 acagcggctt gatctcttct tcccagacgc gaggattgag atcgcccgca tggcgatagc
    12781 gaatgatacc gttgccgtca ataaggaagg tttctggcgc gccatagaca ccgagatcca
    12841 gccctaacat gccgtcgcca tcaaacaggc ttagcgcgta aggattgccc agctctttca
    12901 gccagctgat tgccttctgg cgatcgtctt tatagttcat gccgaccacg cgaatgccct
    12961 gcgctgaaag ctgattcaga tattgatgtt ccgcacggca ggtcggacac caagtcgccc
    13021 agacgttaag cagtactggt ttgccctgag tcagcacatc cgcctgataa aactgccccg
    13081 gattgtccag tgattcgaga cgaaacttcg gcacaggctt gccaatgagc gccgattcca
    13141 gattggtcgg atcatcccct tcggcattac gcgccagctg ccacagcagc gccgcggcaa
    13201 tcgccaggaa gataatcaac ggaattaaca atactttgcg cttcatacgg cctccggcgc
    13261 agttttttgc ggactcacgc gcttacgata gcgaggatca aacagacaca gcagtccgcc
    13321 caacgccatc atcagcccgc ccgcccaaat ccagcgaaca aatggtttgt agtaaagacg
    13381 cacggcccac gcgccgtttt ccagctcttc accgagcgcg gcgtacaggt cacgcgtgat
    13441 gccgccgtca attgccgctt cggtcatcat cgacccggca gtgttgtaat aacgtttttc
    13501 cgcatacagc accgtttccg gtttaccctc tcgcgttacg ccaatagtcg ccacaccgcc
    13561 gcgccagttc gggccagtca cctctttgac atcacggaag gtgaagcgat attcatgaat
    13621 atcgacgcta tcgccggact tcatgcgcac atcacgctca acgctataat tctggctaaa
    13681 ggcaatgcca acaattgtca ctgccagccc caggtgagcc gccaccatcc cccaataact
    13741 gaaggtggtt ttcgtgccgc gtgaaatacg cagcgcagct tccgcagttg ccagcaccgc
    13801 aatccagcag gccattgcca ggccgagcac cgtcatcgcc acaactttgc tttcgaacag
    13861 ccacggcaac agcagagaca gcaccagcgt agagatgaag gcgataatca ataaattgcg
    13921 gattttacgt gggcgatcgc gcccccagcg caccagagga ccgacaccaa gcagtagcgc
    13981 aaacggcacc atcagccagg taaacatggt gttgaagaac ggttcgccaa tcgaaatact
    14041 gcccagtcca agttgcttgt gcaccagcgg cagcagcgtc cccagcaaca ccaccagcat
    14101 cgcggcgacc agcaaaacgt tgttcgctaa cagcaaagat tcccgcgacc acagcgcatt
    14161 gtttacgcgt gagcgaactt tgtgtccacg cgcggcaaac agcagcagcg aaccgccaat
    14221 caccagcacc ataaaggcga ggataaacat accgcgcgcc ggatcagacg caaacgcgtg
    14281 taccgatacc agcacgccgg aacgcacgag gaaagtcccc aacagacaca acgagaaggc
    14341 actgattgcc agcagtaatg tccacgcttt gaagctggcg cgttgttcag tgaccgccag
    14401 tgagtgcatc agtgcagtcc ccaccagcca cggcataaac gaggcgtttt ctaccggatc
    14461 ccagaaccac cagccacccc agccgagttc gtaataggcc catgcggaac cgagcacaat
    14521 gccgagcgtt aggaaaatcc acgccgccag cgtccacggg cgagtaaaac gcgcataagt
    14581 gctgtccaga cgcccgctca gcaaagaagc aatggcaaaa gcaaacgcca ccgagaaacc
    14641 cacgtacccc atataaagca gaggcggatg gaagatcagc cccggatcct gtaacagcgg
    14701 gttaagatcg cgcccttcaa tcgggaagtt cggcagcgtg cgtgagaacg ggttagaggt
    14761 aaagagaatg aacagcaaaa agccgacgct gaccatcccc attatcgcca gtacacgggc
    14821 cacaatatcc agcggaatac gctgactaaa aatcgccacc gcaaaggtcc agccgctcat
    14881 cagcagcacc cacagcagaa gcgaaccttc atgcgcgccc caagtagccg ccacgcgata
    14941 ccacaccgga agctgggtat tggagttgct ggcaacatag gtgacggtaa agtcgttgac
    15001 cacgaaagca ttgaccagca ccagaaatgc gccagccaca gacataaaca gcagccaggc
    15061 aaacaagcgg gaagacgcca tcatgcgcgc atctccgcgc gccacgcccc atagcggata
    15121 cacggacagc agcagcgcaa tccctaacgc caagcacagc agcccgtttc caatttccgg
    15181 catcatgatg ctgggtcctt ataaacactc gccgggcgac ggtggttagc ttccatcgct
    15241 ttctcaactt ctggcggcgt gtagttttcg tcgtgtttcg ccagcacttc tttcgcgagg
    15301 atatgattgc ctttttccag ctcgccctgc accacaacgc cctgcccttc acggaacaga
    15361 tccggcaaaa tgccttcgta agagacatcc actgagcctt cagcatcgta aatggtgaag
    15421 gtcactttca gcgaattggg atcgcgctgc acgctacccg gcatcaccat cccgccaacg
    15481 cgcagacgct gaccgacttc cggcatctgc tgagtttcac gcttgccgta gagaatttcc
    15541 cccggcgtat aaaagagatc gatattcgag cgcagcgcat atagcaccag accgatagtc
    15601 agcgctagcc ctgccaacac ggcacaggca atccacaagc ggtttttacg gcgaatattc
    15661 atgcagcctc ctgctgttgc gcagcacgta aacgcgcctc acgcgcccgc tgttgcgcca
    15721 caccacgcag aattgcgcga tgttgcatca ccgagtgcac gaccaaaacc accagcggaa
    15781 taacggtcat caccaccgcc agccagacaa aaaaggcgta accgcccatt gcgaaaaatt
    15841 cattccagga agcaaatgca ggggtcattt acggcctctt ttcagtatca gttcactcac
    15901 ccacggacgg cgtttttcca tcagcaaaat caaattacgc atccgcatca gcgtcagcgt
    15961 ggcagacagg agcaggaagc caaaaatcga ccagcgcagc ggcgaacgca tcgccggatc
    16021 gatactttgc tgcatccgcg ttgatccctg atgcagggtg ttccaccact ccacggagta
    16081 atgaataatc ggcagattca ccacgccaat cagcaccagg atacctgccg cacggcccgc
    16141 cagacggcgg tcgtcgaagg cgtgccacag ggcaatcaca cccacataca aaaacagcag
    16201 caccagttca gaagtcagac gtgcatccca tacccaccag gtgccccaca tcggttttcc
    16261 ccatgcagag ccggtaacca gggcaataaa ggtaaacacg gcaccaatgg gggccatcgc
    16321 cgccaccgcc aggttggcca ttttcatctg ccagacaagg ccaataaacg ccgccaccgc
    16381 cattgatgca taaatgccca tcgaccagat cgccgcaggc acatgcaggt agataatgcg
    16441 gtagctattt ccctgctgat aatcagccgg agcaaagccg aatccccaga tccagccgac
    16501 ggtaagcacg accacactgg caattgccag ccacggtata aaccagccac agatttgata
    16561 cagccgtggt gggatcgcca gttgatgcag tgttttccac atagtttcga taccagactc
    16621 gaacaaaaat cagtaatcca gcgttattga atgctgattc gtaacgctgc cgccgtcgca
    16681 aaaggactta atgtcgcggt gcctgccagc aacgcgccta aaattgccag atacccatca
    16741 acgggcaaat gcatagaagc cgcgtccatc gcggcggtgg caaagatgag taatgggata
    16801 gtcagcggta acaccagtat gctgagcagc acaccaccgc gcttaagtcc cactgtcagc
    16861 gccacgcccg gtgcaccgag aaagccaagc gtaggcgttc ccagcagcag cgtcagcgcc
    16921 atcacttgcc agccataaac atccattccc agtagcattg ctaccagtgg cgaaaggatg
    16981 agtaacggca gaccggttac catccagtgc gccatcacct tcgccagcac aacggcgggc
    17041 aagggtaacg gcaacaacat caattgttca agactgccgt cctgcaaatc gtcacggaac
    17101 agtcgttcca gcgccagcaa ggatgaaagc agcgcagcaa cccagataat gcccggtgca
    17161 atacgcgcca gcagttgcgg ctccggaccg atactgagcg gaaaaagggt aattacaatc
    17221 aggaagaacc acagcgggtt ggcgatttcg gcgctatggc gaaacgctac gcgcagttca
    17281 agacggaaaa tacgccagaa catcatgcgg ccctcgtttg cgtcagtgaa atgcggcgaa
    17341 ttttactttc agcaacgttg agcggctggt gggtagtcag aatcacaatc cccccctgct
    17401 ccgtatgctg cgccatacgc tgggtcagac gatcgacgcc gttaacgtca atcgcggtaa
    17461 aaggctcgtc gaggatccat aatgtggcac gggtcaacca cagacgcgct aaagcgacgc
    17521 ggcgttgttg cccggcagaa agttgattta ccggaatatc ttcgaatccg gcaagcccgg
    17581 cctgcgccaa ggcttccaga cattgtgcgg catcgccatc gcgatgataa aagtgcagat
    17641 tttctaacgc cgtcagccgg gttttgatcc ccggctgatg gcctatccat aacagggttt
    17701 gatggtagct gtcgcgtacc tgatgcaagg gctgcccttg ccagagaacc tcgccagcgt
    17761 cagggcgaga caaccccgtc agcaaacgga gaagcgttgt cttccccgcg ccgttgctac
    17821 cggtgatttg tacccactct cctgcgttca gcgtaaatga caagccacta aataaggttc
    17881 gttcatcccg ctcacaaagt aactctctgg cttcaagcat acccacgcaa taaccctgtt
    17941 aaaaacctgg ctcgacttca cgcatatcgg gcagcttgtg cgcgatccct ttatggcaat
    18001 caatacaggt ttgcccatct ttcaccgcct ggtcatgcat cttcgcggca accgatttct
    18061 gggcggttgt atccatatac tcgaagttgt gacagttacg gcactcctgc gagttattgt
    18121 ccttcatgcg ccgccactca ttctgtgcca tcgtcagacg atgagcttca aatttctgcg
    18181 gcgtgtcaat aacgccaaaa attttaccat acagctcttt acttgctttg agcttgcgta
    18241 tcatcttcgg cacaaactcg tgcggaacgt gacaatccgg acaggtcgca cggacgccgc
    18301 tacggttgtt gtagtgcacg gaatccatgt attcctgata caccgtgttg cgcatttcgt
    18361 ggcagctaat gcagaactct tcggtattgg ctttttccat cccggtgtta aagccccccc
    18421 agaagacgat gccgccaaca aaaccgatca acagcagcgt ccccagcgcc agatggctgg
    18481 gggtacgcca ccatttccac aggcgcttaa tcagaccagg cttacggtca gaatttccca
    18541 taataacctc ttatttcccg taaccttttg atggggtaaa ggtattcccc acgattggcg
    18601 cggtatcggc ctgcggtacg tgacattgca gacagaaata acgacgcgga gccacttccg
    18661 cgcccacttt gccgtcgctg tccataaagt gagtaggact gatacgcggc gcgccagtgg
    18721 tgcgatagct ttcgacaccg tggcactgca agcagcgatt ggtattggtc gttacctgat
    18781 aaccttcaac gctatgcggg atcattggcg gctgattcac atagttcagc ggcatccgat
    18841 cctgctcttt tggcatccga atggcccctt cctgcgtccc ggatacttcc ggcgactggc
    18901 taaaatcgac gccattagcc gcccaaaccg cgccgcttac caccagggcc agcatcgccg
    18961 tccattgaca cagcgctttc ttcaggtcat ggcttttcat gatttcgctc ccgaactcca
    19021 tcgtgtagtt attgtaaata catcctcaga acagacatcc acgcagcgac cgcacgtcat
    19081 gcaatcgcgg ctggtgacct gcaccgggct ttgttcatcc agcaccgggg cacgtagcac
    19141 atgcggttcc gggcaaacat gaaaacaatc catacagcgg ttacatttct gccgatcgct
    19201 ggccgcaacg gtaatcacac ctttgctacc cagcacgcca tacagcgcgc caaccggaca
    19261 aatgtgcccg caccagccgt gttcaacgac cagtagatca aataaaaaca gcgcgagaat
    19321 aagcagcgcg ccgctgccga agcccattac caggctacgc cccatcagag aaacggggtt
    19381 gatccattcc caaatgagcg tgccagttaa ggctgatccc accagaatga ccaccaacag
    19441 cacgtagcga atgtggcgag ggatcgtcgc agactgattg aggtcaaagc gcctgcgtaa
    19501 ccagttcgct aagtcggtaa tcgggttcag cgggcagacc cagctgcaaa ataatcgctt
    19561 tcccgccagg gcgtagagca cggtgataat caccgcaccg gtcaacgcca ccgtggcagg
    19621 cagatgacca ctggcaagac tttgcagcgt catcagcgga tcggtcagcg gaacggtgtc
    19681 gaataacagg ctactgctgt agttgccgtg caagatccac acaccaaacc atggaccgct
    19741 caaaaacatc cccagcacga agaactgaca aaggcgacgt aacaccagcc aacggtgact
    19801 gcgccaccag ccttttttct ccagcgcctc gcgcccggcg tcacgtttac gatttgccat
    19861 tgttcccctc cagccagccg aagcggtaat ggtgacctaa ctcccctttc gccagtgaca
    19921 gcggtaacac cttgattgcc ggttgttcca gcacgcatac tttttcgcac ttaccgcaac
    19981 cggtacaggc gtcgctgtga accgtcggca gaaagcgggc gtgcttaccg gtacgcgtgt
    20041 tgcgctccag ctccaaggtg atggcctcat caattttcgg gcattcgcga taacaaacat
    20101 cgcagcgcag cccctgaaag ttgagacagt tttcctggtc caccagtacc gccagcccca
    20161 tccgcgcgtc gtcgatcgat tcaatctcac gatccagcgc accgcttggg cacactttgg
    20221 cgcacggaat gtcctcacac atttcgcaag gaatatcccg tgcgacaaaa tatggcgtgc
    20281 ccgccgacag accagaggcc agcgtcgcca gttttaaggt gtcgtaaggg caagcctgaa
    20341 cacactgacc gcaacgcaca caggcactgg caaaggcgtt ctcgtttatg gctccgggcg
    20401 ggcgcaaccg cacgccagat gcgcgtgcgg tttgctgttg taaccccagc gccacaccca
    20461 cggcagccag cccgcctgct gtgcgaacga catcgcgcag aaagcggcgg cgaccatttt
    20521 gaggtttcgc tgaccgggac ataatggctt acaccttctc cagtttgacc gcgcacttct
    20581 tgaagtccgt ctctttcgag agcggatcgg tcgcatccag cgtcagtttg ttaaccagct
    20641 gtgcggcgtc gaagaacggc atgtacacca ggccctgtgg cggacggtta cgaccgcgcg
    20701 tttcaacaat cgagatcact tcgccacggc gagaaaccac tttcactttg tcgccacggc
    20761 gcagatcgcg cgctttcgca tccagcgggt gaataaacaa gaccgcttcc gggaaggcgc
    20821 ggtgcagttc cggcacacgg cgagtcatac tgccggtgtg ccagtgctcc agaacgcgtc
    20881 cggtagagag ccacaggtcg tactcttcat ccggtgcttc cgccgccggt tcgaacggca
    20941 gtgcgaagat caccgctttg ccatccggtt taccgtagaa cttatagcct tcgcccgctt
    21001 tcacgtacgg gtcgttacct tcgctgtaac gccactgtgt ttctttacca ttcaccaccg
    21061 gccagcgcag accgcgcgct ttgtggtagt catcgaacgg tgccagatcg tgaccgtgac
    21121 cgcgaccaaa ccatgcgtac tcttcgaaca gccctttttg cagatagaag cccagctcgc
    21181 gggattcatc gttcagctga tcttccgcca gttcggatac cgggaatttg ctcacttcgg
    21241 gggtggcata cagaacttcg tacagcgttt tgccacgcag ttccggtttc ttcgccagca
    21301 gctcttccgg ccatacttct tcagttttga agcggcggga gaactggact aactgccaga
    21361 gatccgattt cgcttcgcct ggcgcctgta cctgctgacg ccagaactga gtacggcgtt
    21421 cggcgttacc gtaagcgccc tctttctcta cccacattgc ggtcggcagg atcaagtcgg
    21481 cggccagcgc actgactgtc ggatacggat cggagacgat gatgaagttg cgcggatcgc
    21541 gccagcccgg catacgctct tcattgatgt tcggcccggc ctgcatgttg ttggtacaca
    21601 tggtccagta aacattcagc ttgccgtctt tcagcgcacg gtcttgtgcc accgcatgaa
    21661 gaccgatttt cgccggaatg gtgccgctcg ggatattcca cttcttctca cagatatcac
    21721 gatgtttctc gttagttacc accatgtccg caggcagacg gtgagcaaag gtgcccactt
    21781 cacgcgcagt accacacgca gaaggttgcc cggtcaggga gaacggaccg caacccggct
    21841 gggaaatttt gccggtcagc aggtgcaggt tgtagaccag gttgttagcc cacacaccac
    21901 gggtatgctg gttgaagccc atcgtccagt aggagatgac tttcttgttc ggatcggcat
    21961 acagctgcgc cagttgttct aactggtctt tcggcacgcc ggtcatttca gcggtttttt
    22021 ccagcgtata ctcagcaacg aaggctttgt aatcttcaaa gctcatcggt tcggaggcgt
    22081 cagaacccgg attcttcgct gctttttcca gcggatgggt cggacgtaaa ccgtagccga
    22141 tgtccgtcgc ccctttgcgc aggttaacgt gcttgctgaa gaagtcctga tttatcgcat
    22201 tgttttgaat gatatagttg gcgatgtagt tcaggatcac caggtcagat tgcggcgtaa
    22261 agatgatgcc gttatccgcc agctcgaagc tacgatgctg gtaggtagaa agcaccgcca
    22321 cggtgacgtt ctggttagag agacgactgt tagtgatgcg tgaccagagg atcgggtgca
    22381 tctccgccat gttagcgccc cacagcacaa acgcgtcagc ctgctcgatg tcgtcatagc
    22441 agcccatcgg ctcatccata ccaaaggtac gcataaagcc tactactgcc gacgccatac
    22501 agtgacgtgc gttcgggtcg atgttgttcg aacggaagcc cgctttgaac agcttggacg
    22561 cggcataacc ttcccagata gtccactgac cagaaccgaa cataccgatc gattccggcc
    22621 ctttttcttt cagggcggtt ttgaatttct cttccatcac atcgaaggcc tgatcccagg
    22681 tgattggggt aaattcgcct tctttgtcat atttaccgtt tttcatacgc agcagcggct
    22741 gcgtcaaacg gtctttaccg tacatgatct tgggcaggaa atagccctta atgcagttca
    22801 ggccacggtt aaccggtgcg tccgggtcgc cctgacaggc caccacacgc ccctgctgcg
    22861 ttccgaccag aacgccgcaa ccagtaccgc agaaacggca cggcgcttta tcccatttaa
    22921 tggcttcctg ctgaccaaca acggcgcggg caacgcccgg cacgctgaga ccggcagccg
    22981 ccgcagcggc cgcaacggcg ttagctttca taaagctacg acgactgagt ttcatggtgt
    23041 ttcctcacct tgctcttcct gctggtgata aaccagcgac accgccagca cgccctctac
    23101 gttgcgtact gactcaatgg tttggatcag cgtttcgctg tcttctgctt ccaccaccac
    23161 aatcaactga ccgctcggcg cgtcgctgac agcaacttca cagccgggaa aggcgttcag
    23221 ttgggtgctg atgtctgaaa ttcgttcgct tttggcctgc acgaccaggc tgcaaacttg
    23281 ccagttagtg tgcatggaga tactccgcag ttatggctga taccggacag ctggctgcac
    23341 atgctccaca tccgttgcaa agttggctat taagttgcgc ctggtagatc ccggagagcg
    23401 tcgggcgaaa gataattgcc attggctcac agctatcctg acagcggcgg cattcaactg
    23461 actgatacgc caggcacgcg tccccgatgg tgaactgcaa atcccaggcc ctggtgtggc
    23521 gcggagaaaa tagcgattcg gggcaagcct gcgcgcaggc gtaacagaag ctgcactcgt
    23581 tatttttgaa attaacgctc ggatagccac ccgtgccgcg ttgcagaatg ttgttttcac
    23641 aagcattaat acaggcgtcg cagcgggtgc aatgggtcag aaaatgagat tcatcaccgg
    23701 accagggcgg acggataccg ttactggctt tgcgccagca accagtgagt atgccccgac
    23761 gggatgcatc aatcttcaca ttgaccttcc atcattaacg cgctctgaaa attgagagcg
    23821 accaaataga ccgcataatt aataagccat ttttatagcc gctaagatat taaaggatgt
    23881 gtcaaagatg cataccccga tcggggtaaa acctgtagca ggatcaaaag agtggcggga
    23941 agcgtggcaa aaacgggctt ttgctcacat ttcaaatggt tataaacata tttatatagc
    24001 gattaattca ccagagatat ttctgctggt ttgctccctc atcagaattt aacactaaaa
    24061 gagcaggtaa aattgtctga atgttcttta agttattcat aaagcaaatt aataaatctg
    24121 atgaatatgt taaccttcag cgacatcatc ggtgaaaacc tataaatgaa gaaggaaagc
    24181 aaaaaaatga agaccattct acctgaagta ttgtttgccg ctttcgctac cacttccgcc
    24241 tgggcggcag aaagcgtcca gccactggaa aaaatcgcgc cttatccaca agctgaaaca
    24301 ggcatgaagc gtcaggtgat tcagttaacc ccgcaagaag atgaatctac cctgaaagta
    24361 gaactgttaa tcggtcagac gctggaagtc gattgcaatt tgcatcgtct cggcgggaag
    24421 ctggaaagca aaacgctgga aggctggggc tatgattatt atgtctttga taaagttagc
    24481 tcgccggttt ccacgatgat ggcctgcccg gatggcaaga aagagaagaa atttgtcacc
    24541 gcgtatctgg gcgatgctgg aatgctgcgt tacaacagca agctgccgat cgtggtgtat
    24601 acgccagaca atgtagatgt gaaataccgc gtctggaagg cggaagagaa aattgacaac
    24661 gcggaagttc gctaaactgt tgtgaagtac ggcaccccgt tggttggata aggcggttac
    24721 gccgcatcca acatctaacg cccgagccgg ttgcctgatg cgacgctggc gcgtcttatc
    24781 agtatacgcc acatcattac aacgcaatat ccgccacttc tttttgaacg ggttgcggtt
    24841 tcaactgcgg tttcggcgta ctatctgctg cttgcggttt gtcgtatttc agccccagca
    24901 cttcgctggt gtactgcaac tcgcgttctg ttgccgctac atcaccgttc agcttgcgtc
    24961 cataagacgg aacgatcgct ttcaacgtag cctgccattg cgggctggaa acgcgatcgc
    25021 caaatacttt ttccagtaga ttcaacataa tcggcgcggc ggttgacgcc cctggcgatg
    25081 cccccagcag cgccgcaatg gtcccttgct ggtcactgac cacttcggta cccagacgca
    25141 gtacgccacc tttctcggca tcacgcttga taatctgcac acgctgcccc gcttgccaca
    25201 aacgccagtc ctcttttttc gcttgcggat agtactcttt caacgcttca aaacgatcct
    25261 cttcactcaa catcacctga ctcaccagat atttcaccag atcgaaatta tccagcccga
    25321 cgtgcatcat cggcatcacg ttagaggtgg tggtggaact catcagatcc cacaatgaac
    25381 cgtttttgag gaatttggtt gagaaggtgg caaatggccc aaacagcact acgcgtttac
    25441 cgtccagaac acgggtatcg atatgcggaa ccgacatcgg tggtgcgcca acggatgctt
    25501 taccgtaaac tttcgccaga tggcgattaa ccacgtctgg attttccgaa acaaggaact
    25561 gtccgcccac cgggaaaccg gcgtagtctt tcgcttccgg aatccccgat tcctgtaaca
    25621 gcttcagcgc cgcaccgccc gcgccgataa agacaaattt ggcgcggatg ttctgtgcag
    25681 tgccgttttt cagatcggca acggtaacgg tccaggtgtt atcgtcatta cgctttaggg
    25741 cgcggacttc gctgctgagt tgcagcgaga agttagattt cttctgtaag gaagcaatta
    25801 actggcgggt gatctcacca tagttcacat cagtgccaat ttctgttgcg gatcgcgccc
    25861 ttccatcact aacggtgccc actctttgat ctgcgcgtga tcttcagagt aacgcatacc
    25921 gcgaaacagc gagctttgtt gcaacgcggc gtaacgggcg cgcaggaaat taacgttatc
    25981 ctcgccccag acaaagctca tatgcggaac ggtattgata aatgaacgcg gagtacgcag
    26041 cacgccgcgc tcgacctggt gcgcccagaa ctggcgggaa atctgaaatg cttcgttaat
    26101 ggcgactgct ttttcaatac tgatgctgcc atcggcgttt tgcggggtgt agttcagttc
    26161 catcagtgca gaatgcccgg ttccggcgtt gttctagccg ttcgaactct cctgcgcgcc
    26221 cccctccagg cgctccacca tggtcatcga ccattcaggc tccagctcgc gtcaataggt
    26281 ccccaacgtg gcgctcataa tgccgccgcc aatcaacagt acatcagttt cctgctcctc
    26341 ggacgctttc gcttttgccg ccatcgaaac ggcattaagc cccacggcca tcgagaagag
    26401 catggcagtc acttttttca tcttgttaat gccttacttt tagtcgcttt attgcaggtg
    26461 agatttgcgc ggcatc
//
LOCUS       DAZXUK010000027        26426 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZXUK010000027 DAZXUK010000000
VERSION     DAZXUK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26426
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.027"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            41..1023
                     /locus_tag="ACH3L8_000944"
                     /pseudo
     CDS             41..1023
                     /locus_tag="ACH3L8_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            1046..2557
                     /locus_tag="ACH3L8_000945"
     CDS             1046..2557
                     /locus_tag="ACH3L8_000945"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFT4084165.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2582..3580
                     /locus_tag="ACH3L8_000946"
     CDS             2582..3580
                     /locus_tag="ACH3L8_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4084166.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3613..4707
                     /locus_tag="ACH3L8_000947"
     CDS             3613..4707
                     /locus_tag="ACH3L8_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFT4084167.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4719..5591
                     /locus_tag="ACH3L8_000948"
     CDS             4719..5591
                     /locus_tag="ACH3L8_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFT4084168.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5639..6061)
                     /locus_tag="ACH3L8_000949"
     CDS             complement(5639..6061)
                     /locus_tag="ACH3L8_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFT4084169.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6158..7365)
                     /gene="hcaD"
                     /locus_tag="ACH3L8_000950"
                     /pseudo
     CDS             complement(6158..7365)
                     /gene="hcaD"
                     /locus_tag="ACH3L8_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(7375..8187)
                     /gene="hcaB"
                     /locus_tag="ACH3L8_000951"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(7375..8187)
                     /gene="hcaB"
                     /locus_tag="ACH3L8_000951"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFT4084170.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(8184..8504)
                     /locus_tag="ACH3L8_000952"
     CDS             complement(8184..8504)
                     /locus_tag="ACH3L8_000952"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFT4084171.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(8504..9022)
                     /gene="hcaF"
                     /locus_tag="ACH3L8_000953"
     CDS             complement(8504..9022)
                     /gene="hcaF"
                     /locus_tag="ACH3L8_000953"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFT4084172.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(9019..10380)
                     /gene="hcaE"
                     /locus_tag="ACH3L8_000954"
     CDS             complement(9019..10380)
                     /gene="hcaE"
                     /locus_tag="ACH3L8_000954"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFT4084173.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            10516..11406
                     /gene="hcaR"
                     /locus_tag="ACH3L8_000955"
     CDS             10516..11406
                     /gene="hcaR"
                     /locus_tag="ACH3L8_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFT4084174.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            11566..12705
                     /locus_tag="ACH3L8_000956"
     CDS             11566..12705
                     /locus_tag="ACH3L8_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFT4084175.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12697..13977)
                     /gene="csiE"
                     /locus_tag="ACH3L8_000957"
     CDS             complement(12697..13977)
                     /gene="csiE"
                     /locus_tag="ACH3L8_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFT4084176.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(14168..15022)
                     /locus_tag="ACH3L8_000958"
     CDS             complement(14168..15022)
                     /locus_tag="ACH3L8_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFT4084177.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(15167..15970)
                     /gene="suhB"
                     /locus_tag="ACH3L8_000959"
     CDS             complement(15167..15970)
                     /gene="suhB"
                     /locus_tag="ACH3L8_000959"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFT4084178.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            16089..16829
                     /gene="trmJ"
                     /locus_tag="ACH3L8_000960"
     CDS             16089..16829
                     /gene="trmJ"
                     /locus_tag="ACH3L8_000960"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFT4084179.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            17099..17587
                     /gene="iscR"
                     /locus_tag="ACH3L8_000961"
     CDS             17099..17587
                     /gene="iscR"
                     /locus_tag="ACH3L8_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFT4084180.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            17699..18913
                     /gene="iscS"
                     /locus_tag="ACH3L8_000962"
     CDS             17699..18913
                     /gene="iscS"
                     /locus_tag="ACH3L8_000962"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFT4084181.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            18941..19327
                     /gene="iscU"
                     /locus_tag="ACH3L8_000963"
     CDS             18941..19327
                     /gene="iscU"
                     /locus_tag="ACH3L8_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFT4084182.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            19344..19667
                     /gene="iscA"
                     /locus_tag="ACH3L8_000964"
     CDS             19344..19667
                     /gene="iscA"
                     /locus_tag="ACH3L8_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFT4084183.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19763..20278
                     /gene="hscB"
                     /locus_tag="ACH3L8_000965"
     CDS             19763..20278
                     /gene="hscB"
                     /locus_tag="ACH3L8_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFT4084184.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            20295..22145
                     /gene="hscA"
                     /locus_tag="ACH3L8_000966"
     CDS             20295..22145
                     /gene="hscA"
                     /locus_tag="ACH3L8_000966"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFT4084185.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            22147..22482
                     /gene="fdx"
                     /locus_tag="ACH3L8_000967"
     CDS             22147..22482
                     /gene="fdx"
                     /locus_tag="ACH3L8_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFT4084186.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            22494..22694
                     /gene="iscX"
                     /locus_tag="ACH3L8_000968"
     CDS             22494..22694
                     /gene="iscX"
                     /locus_tag="ACH3L8_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFT4084187.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22753..24036
                     /gene="pepB"
                     /locus_tag="ACH3L8_000969"
     CDS             22753..24036
                     /gene="pepB"
                     /locus_tag="ACH3L8_000969"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFT4084188.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            24178..24954
                     /gene="sseB"
                     /locus_tag="ACH3L8_000970"
     CDS             24178..24954
                     /gene="sseB"
                     /locus_tag="ACH3L8_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFT4084189.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(24986..25111)
                     /gene="timP"
                     /locus_tag="ACH3L8_000971"
     CDS             complement(24986..25111)
                     /gene="timP"
                     /locus_tag="ACH3L8_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFT4084190.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(25773..26255)
                     /gene="sseA"
                     /locus_tag="ACH3L8_000972"
                     /pseudo
     CDS             complement(25773..26255)
                     /gene="sseA"
                     /locus_tag="ACH3L8_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT         6248 a         6585 c         7330 g         6263 t
ORIGIN      
        1 agaataagcc gttgttaata aaaaccctac aggaggcact atgcctaaaa aaatgagaac
       61 aacccgtaat ctattattaa tggctacgct attaggtagc gcactatttg ccagggctgc
      121 ggataaagaa atgaccattg gtgcaatata ccttgatacc cagggatatt acgctggagt
      181 gcgtcagggc gttcaggatg cggcaaaaga ttcttcagta caggtacagt taattgaaac
      241 taacgcccag ggggatattt cgaaagaaag tacctttgtt gataccctcg tggcgcgtaa
      301 tgtcgatgcc attattttat cggcagtgtc tgaaaatggc agtagccgta ccgttcgtcg
      361 cgccagtgaa gcgggtattc cggtgatttg ctacaacacc tgtattaatc aaaagggtgt
      421 cgataaatat gtctcggcgt atctggtcgg cgatccactg gaatttggta aaaactgggt
      481 aacgctgccg ccgattattt tattgccaat aaaattgacc agccgaaaat tgccgtcatc
      541 aattgcgaag cctttgaagt ttgtgtgcag cgacgtaaag gatttgaaga agtattaaaa
      601 tcccgcgttc ccggcgcgca aattgtcgct aaccaggaag ggactgtttt agataaagcg
      661 atttccgttg gtgaaaaact gattatctcc acgccggatc tcaacgccat tatgggggag
      721 tcgggcggtg cgacactcgg cgcggtaaaa gcggtacgta atcaaaatca ggccggaaaa
      781 attgctgttt tcggttcgga tatgacaacc gaaattgctc aggagctgga aaacaatcag
      841 gtgctgaaag cggtagtgga tatttccggt aagaaaatgg gcaatgctgt tttcgcgcaa
      901 acattgaagg ttatcaataa acaagccgac ggtgaaaaag tgattcaggt gccaatcgat
      961 ctctatacca aaacggaaga tggtaaacag tggctggcaa cgcacgttga tggtctgccc
     1021 taatcgctga ttcgcagagg tccctatgtt cacggcaaca gaggcagtcc cggtagcaaa
     1081 agtggtggca ggaaataagc gttaccccgg cgtcgttgcg ctggataacg ttaacttcac
     1141 gctcaataaa ggcgaagttc gtgcgctgtt aggtaaaaac ggcgcgggca aatcgactct
     1201 cattcgaatg cttaccggca gcgaacgccc ggatagcggt gatatctgga ttggcgagac
     1261 gcgactggaa ggtgacgaag ctacgttgac tcgccgtgcc gctgaactgg gggttcgtgc
     1321 ggtttatcag gaattaagtc tggtggaagg gctgacggtg gcagaaaacc tctgccttgg
     1381 tcaatggcct cgccgcaacg gcatgattga ttacctgcaa atggcgcagg atgcccaacg
     1441 ttgcttacag gcgctgggcg ttgacgttag tcctgaacaa cttgtttcaa cgctaagccc
     1501 ggcgcaaaag caactggtgg aaattgcacg ggtgatgaag ggcgagccgc gcgtggtcat
     1561 tcttgatgaa cccaccagct cgcttgccag tgcagaagtt gaactggtga tcagtgcggt
     1621 gaaaaagatg tcggcactgg gcgtggcggt gatttatgtc agccaccgga tggaagaaat
     1681 ccgccgcatt gcctcctatg ccaccgttat gcgcgatggt caggtggcgg gcgatgtgat
     1741 gctcgaaaac acctccacgc atcatattgt gtcgctaatg ctagggcgtg atcacgttga
     1801 tattgcgccg gttgcccctc aggaaattgt ggatcaggcc gtactggaag tccgtgcgtt
     1861 acgccataag cccaagctgg aggatatcag ctttacgcta cgtcgtggcg aagtgcttgg
     1921 cattgctggc ctgctggggg cagggcgcag tgaattgctg aaggcgattg ttgggctgga
     1981 gacgtatgaa cagggcgaaa ttgttatcaa cggcgagaaa atcatgcgcc ccgattacgg
     2041 cgacatgctg aaacgcggca ttggctatac gccagaaaac cgcaaagaag cggggatcat
     2101 tccctggcta ggcgttgacg aaaatacagt gctgaccaat cggcaaaaaa tcagcaccaa
     2161 cggtgtgctg caatggtcca ccatccgccg cctgaccgaa gaggtgatgc agcggatgac
     2221 ggtcaaggct gccagtagcg aaacacccat cggcacgctt tctggtggca atcagcaaaa
     2281 agtggtgatc ggtcgttggg tctatgccgc cagccagatt ttgttgctcg acgagccaac
     2341 gcgcggcgtc gatatcgaag ccaaacagca gatttaccgt attgtccgtg agctggctgc
     2401 cgaaggaaaa agcgtggtgt ttatctccag tgaagtggag gaattgccgc tggtgtgtga
     2461 ccgcatcctg ttattacagc acggcacgtt ctcgcaggag tttcactcgc cggtcaatgt
     2521 ggatgagctg atgtccgcca ttctgtctgt gcactgatga attttaccga ggaacctgaa
     2581 gatgtatgct tcgtcattac cattgccgca gggcaagagc gtctcgctca aacaatttgt
     2641 cagtcgccat attaatgaga tcggtttgct ggtggtgatt gccattcttt atctggtctt
     2701 ctccctgaac gcgcctggct ttatctcatt gaataaccag atgaacgtgc tgcgcgatgc
     2761 cgccaccatt gggattgccg cctgggcgat gacgctgatt attatctccg gtgaaattga
     2821 tgtcagcgtt gggccgatgg tggcttttgt ctcggtgtgc ctggcatttt tgctgcaatt
     2881 tgaagttccg ttggcgattg cttgtctgct ggtgttgctg ttaggcgcgc tgatgggaac
     2941 gctcgccggg gtgctgcgcg gcgtgtttaa cgtaccaagt ttcgttgcca cactggggct
     3001 gtggagcgct ctgcgcggaa tggggctgtt tatgaccaac gcactgccag tgccgattga
     3061 cgaaaacgag gtgctggact ggctgggcgg acaatttctc ggtgtgccag tatccgcgct
     3121 gatcatgatg gtgttgtttg cgctgtttgt gttcattagc cgaaaaaccg ccttcgggcg
     3181 ctcggttttt gccgttggcg gtaatgccac ggcggcgcag ttgtgcggta ttaacgttcg
     3241 tcgagtgcgc attcttatct ttaccctttc gggattatta gcggcggtga ccggcatttt
     3301 gttggcggca cgcctcggtt ccggtaacgc aggtgccgca aacggtctgg agtttgacgt
     3361 catcgccgcg gtcgtggtcg gcggtacggc actttccggc ggtcgcggtt ccctgttcgg
     3421 tacattgctt ggtgtgctgg tgattacgct aatcggtaac ggtctggtgc tgctcggtat
     3481 taactccttt ttccagcagg tggtacgcgg cgtcatcatc gtggtggcgg tgctggcgaa
     3541 tatcttgctg acccagcgaa gcagtaaagc gaaacgctaa accttaacgc ccctggcgcg
     3601 ggtgctgcag ggatgaaaat taatcgtatt gttaatgagg gatttatgaa aacgatgctg
     3661 gctgcttatt tactaggaaa ttcaaccgtc gatctgcggg aagttgcggt gccgacgccg
     3721 gggattaacc aggtactgat caaaatgaaa tcctccggga tttgcggaag cgatgtccac
     3781 tatatctatc atcaacaccg tgccacggcg gcggcacccg ataaaccgtt ataccaggga
     3841 tttatcaacg gtcatgaacc gtgcgggcag attgtggcga tggggcaagg ctgccgccat
     3901 tttaaagagg gcgaccgcgt gctggtgtat cacatttctg gctgtggttt ttgcccgaac
     3961 tgccgccgcg gctttcctat ctcttgtact ggcaaaggaa aagcggctta cggctggcag
     4021 cgtgacggcg gtcatgccga atatctgctg gcggaagaaa aagatctgat cctcctgccg
     4081 gatgcgctga gctacgaaga tggtgcgttt atcagttgcg gcgttggcac ggcctatgaa
     4141 gggattttgc gcggcgaagt ttccggcagc gataacgtgc tggtggtcgg tctggggccg
     4201 gtcggcatga tggcgatgat gctggcgaaa ggtcgcggtg caaaacggat catcggcgtt
     4261 gatatgctgc cggaacgtct ggcgatggca aaacagttag gggggatgga tcacggctat
     4321 ttagcaacca ccgaaggtct gccgcagata attgctgaac ttacccacgg tggcgcggat
     4381 gttgcgctcg attgttccgg taatgccgca ggtcgcttgc tggcactgca atccactgct
     4441 gactgggggc gggtggttta cattggtgaa accggaaaag tggaattcga ggtcagcgct
     4501 gatctgatgc accatcaacg gcggattatc ggctcctggg tgaccagtct gttccatatg
     4561 gaaaaatgcg cccacgattt gactgactgg aagctgtggc cgcgtaacgc cattacccat
     4621 cgcttctcgc tagaacaggc aggtgatgcc tatgtgctga tggcgagcgg caaatgcggg
     4681 aaagttgtga ttaacttccc ggattaagga gttagcggat gaccatctat accttatccc
     4741 acgggtcgct aaagctggac gtttctgacc agggcggcgt aatcgaaggc ttctggcgcg
     4801 atacgacgcc gctcttacgt cctggtaaaa aaagcggtgt ggcaaccgat gcctcatgtt
     4861 ttccgctggt gccatttgca aaccgggtga gcggcaaccg ttttgtctgg caggggcgtg
     4921 agtatcaact gcaaccgaat gttgagtggg atgcgcacta tctacacggc gatggctggc
     4981 tgggcgagtg gcaatgtgtc tcgcacagcg atgatagttt gtgtctggtg tatgaacatc
     5041 gcagcggtgt ctatcactat cgggtaagtc aggcgtttca tttaacggcg gatacgctga
     5101 cggtgacgct ctctgtcacc aatcaagggg cagagacgct gccatttggt accggctggc
     5161 atccttattt tccgttgtca ccacaaacgc ggattcaggc gcaggcgagc ggttactggc
     5221 tggaacggga gcagtggctg gcgggtgagt tttgcgagca gctaccgcag gaactggatt
     5281 ttaaccagct cgcgccgttg ccgcgtcagt gggtgaacaa tggttttgcc ggatggaatg
     5341 gtcaggcccg catcgagcag ccgcaggaag gttatgccat catcatggaa acgacgccac
     5401 ctgcaccgtg ttatttcatc tttgtttccg accctgcgtt tgataaagga tatgcgtttg
     5461 atttcttctg tctggaaccg atgagccatg cgccggatga gcatcatcgc ccggaagggg
     5521 gtgatctcat tgcgctagca ccaggggaat caacaacctc agagatgtcg ttgcgggtag
     5581 agtggctgta atttataaaa tgccggataa ggcgttcacg ccgcatccgg catctttcct
     5641 aacgccgatc gagagaaatt gcccccggtc cggtaattgc caatagcaag aacgcgccag
     5701 cgatactgac attcttccag aaattaatca tatttggccc aaccgcatcg ccggtcatat
     5761 cccagtaatg gtgaccaatc accgccgtac ccagcgtgta gaaaataaac agcaccgcca
     5821 gcggacgggt gaaaaagcca agcacgatta atatcgcggc gggcacttcc ataactaccg
     5881 caataatcgc tgccagcatc ggcattggcg cgcccaacga ggccatatat tggaccgtac
     5941 cgtcaaagcc catcattttg ggaaaaccaa aaataatgaa aattaagacc acggcgatac
     6001 gggcaattaa taacaaaacg gggcgggcag ctccaaaatc aaaataacgt agtgtgttca
     6061 taaccaatcc ctttgcaaga taatgtgatt taaacgtaat acacactctt gcacctcgcc
     6121 acgccccagg cattgagata ttcgctaatt atcctgatta cagtgattta agcgcgatgt
     6181 tctcatctat cagcagtttc gcatcaaacg ttttgccgct ctggatccat ttgcgaattg
     6241 ggcgaatctc acggccctga ttcagcgtga ccgcaccgat aagcacgccg ttttgcagat
     6301 taaaccaaat cgccttctga gtttccgggt tgccacgaca aagccagtca tcgccacgca
     6361 tatcgccaat aaactgtaag ttatcactgt actgatcgct ccagaaccac ggcggcggca
     6421 gtagcggtag cggtagccct aacattgcgg cagcggcaat ttgcgcctgg ttattggcgt
     6481 tttcccagct ttcgcagcgg tgtagtgcac cattatcaag acgagtgatt gccacatcgc
     6541 caccggcaaa gatcgcggga tcgcaggtgc ggcaagcctc atcaatgaca atgccattgg
     6601 cagtatcaag gttggcctcg cgagccagtt gctcgttggc gctgatacca ataccgtaaa
     6661 tcaccacatc agcctgaagc gtttccccac tttgcagcgt cagttctact ttttcaccat
     6721 cgaccacatg ttcaatggca ttattgagca gaatgcgcac accagcctgc tgatggcgct
     6781 gtaaaagata gcgttgcacg ggcggtggtg cattacggcc catgacggtt gccgccagtt
     6841 caatcactgt caccttacat ctgcgctgcg ctgcgtggcg ctggcagcca gttccagacc
     6901 aatagttccg gcaccgacaa tcacgactga ccgttcgggc tgcagaactt ctcgcagtct
     6961 ggcggcatca ccggcatggt gtagggtaaa gcagcgttct cccagtgcat caagcaacgg
     7021 cagcggtcga gctgccgcgc cggttgctat aaaaagctga tcccagtgcc agctttcgcc
     7081 gttggttaac actaactctc gtgtgtcgcg acccagcgtt ttgatggtta caccggaatg
     7141 cagatgaaca ttgttttcct gccaccagtt agcgggtaac acctgctgta actgcgggga
     7201 atcttccagc aacatggatt tcgagagtgg aggtcgttca taaggaagat gtcgctcatc
     7261 ggaaaacaga tgcagctcac cggtgaaccc ttgctggcgt agcgaggccg cagccattgc
     7321 cgccgcttgc ccgccaccga caataatgat cgttttttct ttcatcgtgc ttccttaaag
     7381 atccagccca gccgctacgt ggcgaatgcc gcgaatcgct aaacccgcat cagcgttgat
     7441 catcacaccg cttaatgcgc gattattgcg ccgcgatgcc aacatcacat acggccccgt
     7501 aaaatccgcc ggttgcggga aaaattgcag cggtaaaatg gcggcaattt tctccggcgt
     7561 cagagactgc attatcgagg tttcactttg cccgagcgcc tgtgggccgc gcaggtcgct
     7621 ggccatacca cacgggccga cgccattcac ccgcactttc ggtgccagtt cataagccag
     7681 ttggcgaata agtccggttg cggcatgttt actggtggtg tacagcgggc caccgccgcc
     7741 aggataccag gcggcatttg acagtgtgaa aatcatgctg ccttcactgg cgattaacgc
     7801 cggagcgcag gcttttgcgc ccagcaggta accgagtacg ttgacgttaa acagctcgtg
     7861 gaagccggtt tcgagcgtct ctgcgggagt attaaccagt gaggcattgt gatcccagat
     7921 gcctgcattg ccgataaaac aatccagctt gccggaacga gtcaggatct gatcgaccgc
     7981 gcgttgataa tcggcataac aggtcacgtt accttccacc gccagaatat gttcgccaaa
     8041 tcgctgacgc agactggcga cttttgccgc cgacagttcc agcgtggcaa cttgcgcgcc
     8101 ttcttcgata aatcgctcga ccagcgccag ccctaatccc gatccgccgc cggtaataaa
     8161 aatggactcg ttatgcagat cgctcatggc tgcgcctccg gtaagtcgat gaaaatgtca
     8221 ccaccttcaa cgtgtactgg ataagtggtg agcggatcgg tggcgggcag gcataacgct
     8281 ttccccgttt tcaggcaaaa actggcggcg tgtagcgggc actccaccgt ggcgtcatct
     8341 tccagatacc cttctgacat cgacgcatta ccatggctgc aacgatcgtt aatggcataa
     8401 aactcgccgc ccacgttgaa caaggcgatg acgggcgagg tatctatccg gagagcctca
     8461 ccctccggca catccgcaac gggacacgca taaattcgat tcatcagaac agtacactca
     8521 ggttatggga agtgatcacc gcttgatcca ggacgatatc ccgttccagc aagcgccagt
     8581 tgtcatcttc cagacggcga actttgtcga aacgcgttcc aacatagaat gtttcatcgc
     8641 gctctttttg tgcccgataa agtagataat tcaccctcac ggcaaatacg tttgggatgt
     8701 cggtttcgct tacctggcag ttgctgatta agtgacgagt gcgtgacggc ggctcttctg
     8761 cccaggccat gcccgtttcc agacgggcga ttcgccgctc cagctggtct ttggtgtcat
     8821 tgaaaatcca ggtcgtcggc ggctggacgc ctttgcgacg gtcgcgtgtt tgcgcgttaa
     8881 ccgtggtgcg catggtgtaa cgaatctctt cgtcgagctg tgccaaccag tcacgaaatt
     8941 tccagtcatc cagcagactg gcttcgtgaa agagaaactg gctaatgcgg tgatgtaact
     9001 ccagtgaaac ttgcgcactc atttcatcac ctcctgctgg taagcggcgg ttttatcgag
     9061 cacttcctgc cagctttcgc tactcagaag atcggcccag cgttggtaca ttccacgagc
     9121 ggcagtttct gaaaagatat agttagtaat gccaggaatg ccgtcgtcgc ggcgcttttc
     9181 ctgaccaagc cccatttcca gacacagttt gctgttgcgg gcgcggtggc ctttaagcaa
     9241 tttctggatt tcacaccagt tctccgagtc atcctgctcg agaaaaccag caggaccaaa
     9301 agcacgagtg gcgctgtttt caaaagcggc tttaacttca tcggaggcgg ctttgtcagt
     9361 aatacagaac gcccacactt caacttgatc agggccgcgc ggatgccaga cgcggagtgt
     9421 ggcagtgccg tttagccatg aaagcgtggg gaatatattg ttatgacccg ccaggcgcag
     9481 ggcgcgaact tcacctaaac gttgttctgc ttcggcatag gtttcgcggt aatagcttga
     9541 aactgcgcca tcgacccaga cattagcatc cggtttttca gtaaagaaga aaccgctacc
     9601 gtgaccgtcc tgaccaaatt gcagcgcatc tttggcggtt tcccacaccg ggcgggcggt
     9661 ttgtccatca ccgaggcgct tatcgctgcc atcatctttc gcccctaata cctgaacggc
     9721 agaagcatgg ctgaacagag catgatactg gtcactggcg aactgctctg ccgggaattt
     9781 ccagttacag ttgatcaccc acttttgtac gccgccgaca atttcggtgc cgccttcgcg
     9841 acgatccagc atgccatcca gataccaggc aatgtcaccc aggtaatcat gcaggcccgg
     9901 tgcgctggta tcccagttgc caaaaatcag tcctttataa ctctccacac aaggaacttc
     9961 gtttagcccc cagtgggatt tacacaaccc ttgtgggtag gcgcgaggtt ccagcggtac
    10021 atcgatcaac tcgccgttaa tgccataaga ccagccgtga tacgggcagg taaaggcgcg
    10081 actgttgcca caatctgcat aactcacacg catggcccgg tggcggcatt ggttgagaaa
    10141 cgccttgatg ctgccgtctt tctggcgcac cacgacaact gcatcttctc ccatgtaggt
    10201 gttaaagaaa tcaccgggtt ttgggatctg gctttcgtgg gcgagaaata gccagcaacg
    10261 accgaaaata cgctcaagct ccagttggta aatgtccggg tcggtataaa tacgcggagt
    10321 gacccgaccg ttttgggtgt caatcagttg gtaaatgttc aaatctgagg gtgtggtcat
    10381 attttttaat cctgaaagat aagatgtatt gaccttctat ttttgtgcca taaggatgtt
    10441 gctggttggt tgctaatatg tgaaatagtt aatttctacc agctgagacg ttttttcgat
    10501 aacaagggga aggtgatgga actacggcat ttacgctatt tcgtcgcagt ggcgcaggca
    10561 ctgaacttta cccgtgcggc ggaaaaactg catacctcac agccttcgtt aagcagccag
    10621 atccgcgatc ttgaaaactg tgtcggtgtt ccgttactgg tgagggataa gcgcaaagtc
    10681 gcgttgacgg cggcgggaga atgttttctc caggacgcgc tggcaatcct cgaacaagcg
    10741 gaaaatgcca aattacgggc gcggaaaatt gttcaggaag acagacaatt aaccattggc
    10801 tttgtgccat cggcggaagt gaatttattg ccaaaagtat taccgatgtt tcgtctcaga
    10861 cagccagaca ccttgattga gctggtgagt ttaatcacca cgcaacagga ggaaaaaatt
    10921 cgccgtggtg aactcgacgt cggcttaatg cgccatcctg tttatagccc ggagattgat
    10981 tatctggagc tttttgacga accattagtg gtggtgttac cggttgatca ccctttagca
    11041 catgaaaaag agatcaccgc cgcgcaactg gatggcgtga atttcgtcag taccgatccg
    11101 gcgtattccg gttcgcttgc gccgatcgtt aaagcgtggt ttgcgcaaga aaacagccag
    11161 ccaaatatcg tccaggtggc aacgaatatt ctggtgacca tgaatctggt gggcatgggg
    11221 ctgggcgtca ctttgatacc cggttatatg aataatttta ataccgggca ggttgttttt
    11281 cgtcccattg ccggaaatgt gccttccatt gctttactga tggcgtggaa gaaaggggag
    11341 atgaagctgg cattacgcga tttcatcgcc attgtgcagg aacgtttggc aagcgtaacg
    11401 gcataattac gcattatttt tgcaacatgc gtcgcggatc gcagtttttt agtgcttgtg
    11461 aactggcgtt acagacatcg ggaagcaggc tcgaatattc ttatttttca acttgcacca
    11521 acattcgatt aaccccagac taacgcctcc tgacgggagg gactcatggt tttgcaatcc
    11581 acgcgctggt tggcgctcgg ctatttcaca tactttttta gttacggcat ttttttacct
    11641 ttctggagcg tctggcttaa agggattggt ttaacgccag aaaccatcgg cctgttattg
    11701 ggggcaggtc tggttgcccg ttttctcggg agtttgctca tcgcgccccg cgtcagcgat
    11761 ccttcccgcc tgatttccgc cttgcgcgtg ctggcactgc tgccacttct ctttgctgtc
    11821 gccttctggg cgggggcgca cgtagcgtgg ctgatgctgg tgatgattgg ctttaacctc
    11881 tttttctcac cgctggtacc gttgaccgat gcactggcga atacgtggca aaagcagttc
    11941 ccgcttgatt acggcaaagt gcgactgtgg ggctcggtgg cgtttgtcat tggctcggcg
    12001 ctgacgggca aactggtcac tatgtttgat tatcgggtga tcctcgtgct gttgacgttg
    12061 ggcgtggcat ccatgctgct cggctttctc atccgtccga cgattcagcc acaaggggca
    12121 agccgccagc aggagagcac cggttggtct gcgtggttgg cgctggttcg ccagaactgg
    12181 cgctttctgg cctgcgtttg tttattgcag ggggcacatg cggcctatta cggttttagc
    12241 gccatttact ggcaggcagc tggctactcg gcctcggcgg tggggtattt gtggtcgctg
    12301 ggcgtggtgg cggaagtcat tatctttgcg ctgagtaata aacttttccg ccgttgtagt
    12361 gctcgcgata tgctgttgat ctcggcgatt tgcggcgtag tgcgctgggg cattatggga
    12421 gcaactacgg cgttgccgtg gttgatagtg gtgcaaattc tgcattgcgg caccttcacg
    12481 gtctgccacc tggccgccat gcgttatatt gctgctcgcc aggttagcga agtcatccgt
    12541 ttacaggcgg tttactctgc cgtcgcgatg ggcggcagta tcgctatcat gaccgttttc
    12601 gccggtttcc tgtatcaata tctgggccac ggcgtgttct gggtaatggc gctggtggcg
    12661 cttccggcaa tgtttttgcg cccgaaagtt gttccctcat gctgattcga gcattttgcg
    12721 gatctgctcc tgttgatgtg ttgtcagcgt caggtccgta tagatcagcg gtggtgaaaa
    12781 gagcggtaac ggcatggtat aaggcgcaat aatcagtgcc gcgccacgcg gagcgcctgt
    12841 ctgcaaaaat gcctttaccg acatatgctt aatgttgagc ggcagtaacg ttagttcgcg
    12901 taactgctgc tcaatctgcg cttctcgctc gctatcattc ccggtcagta gaataatctg
    12961 tttttcatgc agatcgtttt cctgcattag ccaggcaccg aaaatcaccg cgaccagacc
    13021 actttcctca tcagaaagat ggacgccgta ttcactttca aatccggcca gcgccgcgcg
    13081 ggtggtgcgt accaggcgtg gatacagtct ggcgaactct tccggcaggg tattatcaat
    13141 accgatggca aacaaactgc gatttaacgc ctgggcgagg tggatataaa gctgatcaca
    13201 taacccctgt tcatcataaa aacgaacatt tcccagctca cgaaaatggt ttaccagacg
    13261 tttgatagac tgacgcaatt gtctgtcccg ctgatgcgca tcccgcagtg gatcgggaac
    13321 ccgcaacatg gaaaaaagta atgccataaa cagtggctca tcaggcggta catgctggag
    13381 cgcccgacgc tgccagtggc gtccaatttc ctgcgctacc tgaaattcaa ggcaggattc
    13441 cgcccagcga cgttggagcg gattaaactg aggcgtaatt ccagcgtggt gctgcagcaa
    13501 acaatattgc agatacagac acaggaaatg aatatcgcgc gattcaaagc gtttttgcag
    13561 ccgtcgggag cagagattca ctaacgcttg tagattggta tcgtcgtaaa agttacgcgc
    13621 aattccgcgc cgcttcagtt cactcttcag ggcgggggtg aattggctgg taataaatga
    13681 cggacacaga cggaaaccac gtcgtagcca gtgaaataaa cacaaacgtt ggttaagcac
    13741 tgtaccttca acccggtagc taccgtcata accagtcgtc agtgtgagtt gatgatagcg
    13801 caggatctcc cgtcctgttt cgctgatatc aagactggca atatcatcat ccacaccatt
    13861 aagctcgctg aaggttgcca tggtggcggt caaccccggc tgaaagagcg tcagcaagat
    13921 ctggcagcgg cgctggggag ccgaaaggac agatggtgga gcaagcgtag gcatcatctg
    13981 tagatctccc ataagcataa ttgataagaa tagctaaagt ttgctcaggg aaatggcgaa
    14041 gggaatgcta atcatcagaa atgttggcaa gcgtcacaga atttttctca ttttaaactg
    14101 ttctgaccag tggattagca tgtcttaagg tggttttgtg aatgattatc gtgctagcga
    14161 tcatgggtta gttctgcgga ttcaacgcgc taaggataaa gtccaccatc tgttcgcgat
    14221 aaacattgcc gtgacgatcg gaaaaagcat caatgtgttc gccatccggg attaggatca
    14281 accgttttgg ctcttttgcc aggctataca acttttcgct gtgctgccat gggataacgt
    14341 gatcggcttt accgtgaatg agtaaaagcg ggatcgggct gacgctggcg atgtaatttt
    14401 cgccgctgta actctcatca agtaagtagc cactgccggg gatcatctgg ttggcgatgg
    14461 ttgcataaga ggcaaatgtg gagtcgagga tcaccgcacg tatgccttca cgatcaccct
    14521 gaccaataac agcaagaata ttcgccccgc caatactctg cccgaacagc accagctgtt
    14581 gtgggtttac atcactgcga tggcgcacca cattgatggc gctttgcgta tcgtccagca
    14641 atccggcctg ggacggagtg ccttttgatt taccaaaccc gcgataatca aacataaaaa
    14701 cgttgaaatt acgctcaggt aaccaactga ccagcggcca gtgggcggac atatttccgg
    14761 cattgccgtg agcatgaatg atggttgcga tggcgttgtc agcagggccc gtcgaagaag
    14821 gaataaacca gccttgcaga cgagtaccgt ccttagccgt aaattcgacg gattccgccg
    14881 accaggggtc gggaccgtaa attttatcgt caggataata gaagaagttg atggcaacgc
    14941 gcggcactaa ataaacgcag aaggcaacaa caaagagaat aaacagaatt ttgggaacgc
    15001 gtttgttcac tggcagtgcc ataacctttc cttgcgactt ctttatcatc cattaatcgg
    15061 ggaaagatgg tgggagagtg acgaaagaaa gtaaagcggt gaggaagtat ttcggaatag
    15121 cgcctgaaag gcgagggcgg gtgagtgata tcacccgcct gagtcattaa tgcttcagag
    15181 cgtcgcttaa ctcgtcacgc atgttcgcca gtatggcttt cacaacacgc gggttaccag
    15241 caacgatgtt accggtcagc atatagttat gaccaccggt gaagtcgctg acgatgccgc
    15301 ccgcttcacg aaccagcagc tcgcctgccg cgaaatccca cggacgcaga ccgatttcaa
    15361 agaaaccgtc aacacgaccc gcagcgacgt aagccagatc cagcgccgca gaaccggtac
    15421 gacggaagtc tgcacattcg ttgaacagct tgccgacgat gttgatgtag gtagtggcgt
    15481 actgttttgc tttgaacggg aagccggtcg ccagaatggt accgtcgaga tcgcgagcgg
    15541 tgctaccgcg cagacggtag ccgttcagct gtgcgccttg accgcgagtg gcggtgaaca
    15601 gttcgttacg cataggatcg tataccacag caacttcggt gcggcctttg atacgaacgg
    15661 cgatagatac cgcgaagtgc ggcagacgtt tgataaagtt ggtagtacca tccagtggat
    15721 cgataaccca ttgaacatcc tgatcagtac cttcaagttc accgctttct tcggtgatga
    15781 tggtgtgctg tgggtaagat ttacgaatcg tgtcgataat caccgcttcg gcagctttat
    15841 ctacgttggt cacgaaatcg ttactgcctt tctggctcgc ttctacagcg tccggggttt
    15901 catagttttt ggcaattaaa ttacccgcct tgcgcgctgc gcgcacggcg atgttcagca
    15961 tcggatgcat cggtctctct cactggatgt taaagaacgg gaaaacggcg cagagtatag
    16021 cagcgagatc ggcatctgtc ttcgttttat gataagatgc gtgaataatc tttagatgaa
    16081 gaaagacaat gctgcaaaat attcgaattg tgctggtgga gacgtcacac accggcaata
    16141 tgggttctgt tgcccgtgcc atgaaaacaa tgggattaac caatctgtgg ctggttaatc
    16201 cactggtgaa acccgactcc caggcgattg ccctggctgc aggggccagc gatgtgattg
    16261 gtaatgctca catcgtcgat acgctcgacg aagcgttagc cggttgtagc ctggtggtgg
    16321 gcaccagcgc acgctcccgc acgctgccgt ggccgatgct cgacccgcgc gaatgcggtc
    16381 tgaaaagcgt cgctgaagcg gcaaataccc cggtggcgct ggtgtttggt cgtgagcgcg
    16441 tcggcttgac caatgaagag ttgcagaaat gccattatca tgtcgcgatt gcggcaaacc
    16501 cggaatacag ttcgctgaac ctggcaatgg cggttcaggt tattgcctat gaagtgcgta
    16561 tggcctggct ggcaactcag gaaaatggcg agcaggtcga gcatgaagag acgccgtatc
    16621 cgctggtcga tgatctggag cgtttttatg gtcatctgga acaaacgctg ctggcaaccg
    16681 gttttatccg tgaaaaccat ccggggcagg tgatgaataa attgcgccgt ctgtttaccc
    16741 gtgcgcgccc ggaaagccag gaattgaata tcctgcgcgg gattctggct tctattgagc
    16801 agcagaataa aggtaacaag gccgaataac agtggttgcc tgatgcgacg cttaacgcgt
    16861 cttatcaggc ctacaatgta cagaatcgta ggtcggataa ggtgttcaca ccgcatccga
    16921 caacaggtac aaacgccacg ataaaaaaat ggcactgaag gttaaatacc cgactaaatc
    16981 agtcaagtaa atagttgacc aatttactcg ggaatgtcag acttgaccct gctatgcaat
    17041 acccccactt ttacaataaa aaaccccggg caggggcgag tttgaggtga agtaagacat
    17101 gagactgaca tctaaagggc gctatgccgt gaccgcaatg cttgacgttg cgctcaactc
    17161 tgaagcgggc ccggtaccgt tggctgatat ttccgaacgt cagggaattt ccctttctta
    17221 tctggaacaa ctgttttccc gtctgcgtaa aaatggtctg gtttccagcg tacgtggacc
    17281 aggcggtggt tatctgttag gcaaagatgc cagcagcatc gccgttggcg aagtgattag
    17341 cgccgttgac gaatctgtag atgccacccg ttgtcagggt aaaggcggct gccagggcgg
    17401 cgataaatgc ctgacccacg cgctgtggcg tgatttgagc gaccgtctca ccggttttct
    17461 caacaacatt actttaggcg aactggttaa taaccaggaa gtgctggatg tgtctggtcg
    17521 tcagcatact cacgacgcgc cacgcacccg cacacaagac gcgatcgacg ttaagttacg
    17581 cgcttaataa aaagaattca gaatcaggcc ggagtgctaa atactccgta aacacggtcg
    17641 tacatccagc cggtagcctg attccttgca ttgagtgatg tacggagttt atagagcaat
    17701 gaaattaccg atttatctcg actactccgc aaccacgccg gtggacccgc gtgttgccga
    17761 gaaaatgatg cagttcatga cgatggacgg aacctttggt aacccggcct ctcgttctca
    17821 ccgtttcggc tggcaggctg aagaagcggt agatatcgcc cgtaatcaga ttgccgatct
    17881 ggtcggcgct gatccgcgtg aaatcgtctt tacctctggt gcaaccgaat ctgacaacct
    17941 ggcgatcaaa ggtgcagcca acttttatca gaaaaaaggc aagcacatca tcaccagcaa
    18001 aaccgaacac aaagcggtac tggatacttg ccgtcagctg gagcgcgaag gtttcgaagt
    18061 cacctacctg gcaccgcagc gtaacggtat tatcgacctg aaagaactcg aagcggcgat
    18121 gcgtgacgac accatccttg tttccatcat gcacgtaaat aacgaaatcg gcgtggtgca
    18181 ggatatcgcg gctatcggcg aaatgtgccg tgctcgtggc attatctatc acgttgatgc
    18241 aacccagagc gtgggcaaac tgccaatcga cctgagccag ttgaaagttg acctgatgtc
    18301 tttctccggt cacaaaatct atggcccgaa aggtatcggt gcgctgtatg tgcgtcgtaa
    18361 accgcgtgta cgcatcgaag cgcaaatgca cggcggcggt cacgagcgcg gtatgcgttc
    18421 cggcactctg cctgttcacc agatcgtcgg aatgggcgaa gcctatcgca tcgcaaaaga
    18481 agagatggcg accgagatgg aacgtctgcg cggcctgcgt aaccgtctgt ggaacggcat
    18541 caaagatatc gaagaagttt acctgaacgg tgacctggaa cacggtgcgc cgaacattct
    18601 caacgtcagc ttcaactacg ttgaaggtga gtcgctgatt atggcgctga aagacctcgc
    18661 agtttcttca ggttccgcct gtacgtcagc aagcctcgaa ccgtcctacg tgctgcgcgc
    18721 gctggggctg aacgacgagc tggcacatag ctctatccgt ttctctttag gtcgttttac
    18781 tactgaagaa gagatcgact acaccatcga gttagttcgt aaatccatcg gtcgtctgcg
    18841 tgacctttct ccgctgtggg aaatgtacaa gcagggcgtg gatctgaaca gcatcgaatg
    18901 ggctcatcat taatcggtat cggaatcagg agaatttata atggcttaca gcgaaaaagt
    18961 tatcgaccat tacgagaatc cgcgtaacgt gggttccttt gataacaacg acgagaacgt
    19021 cggcagcggc atggtgggtg caccggcctg tggcgacgta atgaagttgc agattaaagt
    19081 caacgatgaa ggtatcattg aagacgcgcg ttttaaaact tacggctgcg gttccgctat
    19141 cgcttccagc tccctggtca ccgaatgggt gaaagggaag tctctcgacg aagcgcaggc
    19201 gatcaaaaac accgatattg ctgaagaact tgaactgccg ccggtgaaaa ttcactgttc
    19261 tattctggca gaagacgcga tcaaagccgc cattgcggac tataaaagca aacgtgaagc
    19321 aaaataagag ttgaggtttg gttatgtcga ttacactgag cgacagtgca gcagcgcgag
    19381 taaatacctt tctggctaac cgcggcaaag ggtttggcct gcgtctgggc gtgagaacct
    19441 ccggatgttc aggtatggct tatgtactgg aatttgttga cgaaccgacg ccggaagaca
    19501 tcgtgtttga agacaaaggc gtgaaagtcg tggtcgatgg caaaagcctg caatttctgg
    19561 acggtacgca gctggacttc gtaaaagaag gcctgaatga agggtttaaa ttcaccaacc
    19621 cgaacgttaa agatgagtgt ggttgcggcg aaagcttcca cgtttgatgc gcataccgat
    19681 aaccccaccg tggtcgcctg cgcgtggggt ttgttttacc tgattcgccg ttatcgcggc
    19741 ggatcgcagc cctgagaatg ttatggatta cttcaccctc tttggcttgc ctgcccgtta
    19801 tcaactcgat acccaggcac tgagcctgcg ttttcaggat ctacaacgtc agtatcatcc
    19861 tgataaattc gccagcggaa gcctggcgga acaactcgcc gccgtacagc aatctgcaac
    19921 cattaaccag gcctggcaaa cgctgcgtca tccgttaatg cgcgcggaat atttgctttc
    19981 tttacacggt tttgatctcg ccagcgagca gcatactgtg cgcgacaccg cgttcctgat
    20041 ggaacagttg gagctgcgcg aagagctgga cgagatcgaa caggcgaaag atgaagcgcg
    20101 gctggaaagc tttatcaaac gtgtgaaaaa gatgtttgat acccgccatc agttgatggt
    20161 tgaacagtta gacaacgagg cgtgggacgc ggcggcggat acggtgcgta agctgcgttt
    20221 tctcgataaa ctgcggagca gtgccgaaca actcgaagaa aaactgctcg atttttaatt
    20281 tctggaagct aaacatggcc ttattacaaa ttagtgaacc tggtttgagt gctgcgccgc
    20341 atcagcgtcg tctggcggct ggtattgacc tgggcacaac caactcgctg gtggcgacag
    20401 tgcgcagcgg tcaggccgaa acgttagccg accatgaagg ccgtcacctg ctgccatctg
    20461 ttgttcacta tcaacagcaa gggcattcgg tgggttatga cgcgcgtact aacgcagcgc
    20521 ttgacaccgc caacaccatt agttctgtta aacgcctgat ggggcgctcg ctggctgata
    20581 tccagcaacg ctatccgcat ctgccttatc aattccaggc cagcgaaaac ggcctgccga
    20641 tgattgaaac ggcggcgggg ctactgaacc cggtgcgcgt ttctgcggac atcctcaaag
    20701 cactggcggc gcgggcaact gaagccctgg caggcgagct ggatggtgta gttatcaccg
    20761 ttccggcgta ctttgacgat gcccagcgtc agggcaccaa agacgcggcg cgtctggcgg
    20821 ggttgcatgt tctgcgctta cttaacgaac cgactgctgc ggctatcgcc tacgggctgg
    20881 attccggtca ggaaggcgtg atcgctgttt atgacctcgg tggcgggacg tttgatattt
    20941 ccattctgcg cttaagtcgc ggcgtgtttg aagtgctggc aaccggcggt gattccgcgc
    21001 tcggcggcga tgatttcgac catctgctgg cggattacat tcgcgagcag gcggacattc
    21061 ctgatcgtag cgataaccgc gttcagcgtg aactgctgga tgccaccatt gcagccaaaa
    21121 tcgcgctgag cgatgcggac tccgtgaccg ttaacgttgc gggctggcag ggcgaaatca
    21181 gccgtgaaca attcaatgaa ctgatcgcgc cactggtaaa acgaacctta ctggcttgtc
    21241 gtcgcgcgct gaaagacgcg ggtgtagaag ctgacgaagt gctggaagtg gtgatggtgg
    21301 gcggttctac tcgcgtgccg ctggtgcgtg aacgggtagg cgaatttttc ggtcgtccac
    21361 cgctgacttc catcgacccg gataaagtcg tcgctattgg cgcggcgatt caggcggata
    21421 ttctggtggg taacaagcca gacagcgaaa tgctgctgct tgatgtgatc ccactgtcgc
    21481 tgggcctcga aacgatgggc ggcctggtgg agaaagtgat tccgcgtaat accactattc
    21541 cggtggcccg cgctcaggat ttcaccacct ttaaagatgg tcagacggcg atgtctatcc
    21601 atgtaatgca gggtgagcgc gaactggtgc aggactgccg ctcactggcg cgttttgcgc
    21661 tgcgtggtat tccggcgcta ccggctggcg gtgcgcatat tcgcgtgacg ttccaggtcg
    21721 atgccgacgg tcttttgagc gtgacggcga tggagaaatc caccggcgtt gaggcgtcta
    21781 ttcaggtcaa accgtcttac ggtctgaccg atagcgaaat cgcttcgatg atcaaagact
    21841 caatgagcta tgccgagcag gacgtaaaag cccgtatgct ggcagaacaa aaagtagaag
    21901 cggcgcgtgt gctggaaagt ctgcacggcg cgctggctgc tgatgccgcg ctgttaagcg
    21961 ccgcagagcg tcaggtcatt gacaatgctg ccgctcacct gagtgaagtg gcgcagggcg
    22021 atgatgttga cgccatcgaa caagcgatta aaaacgtaga caaacaaacc caggatttcg
    22081 ccgctcgccg catggaccag tcggttcgtc gtgcgctgaa aggccattcc gtggacgagg
    22141 tttaatatgc caaagattgt tattttgcct catcaggacc tctgccctga tggcgctgtt
    22201 ctggaagcta atagcggtga aaccattctc gacgcagcgc tgcgtaacgg tattgagatt
    22261 gaacacgcct gtgaaaaatc ctgtgcttgc accacctgcc actgcatcgt tcgtgaaggt
    22321 tttgactcac tgccggaaag ctcagagcag gaagacgaca tgctggacaa agcctgggga
    22381 ctggagccgg aaagccgttt aagctgccag gcgcgcgtca ccgacgaaga tttagtggtc
    22441 gaaatcccgc gttacactat caaccatgcg cgtgagcatt aacagaggtt agtatgggac
    22501 ttaagtggac cgatagccgc gaaattggcg aagcactgta cgatgcgtat cccgatcttg
    22561 atccgaaaac ggttcgattc accgatatgc atcagtggat ttgcgatctg gaagatttcg
    22621 acgacgaccc gcaggcatcc aacgagaaaa tcctcgaagc gattttgtta gtctggctgg
    22681 acgaagccga gtgaatgaac gggctgcctg ggggtagccc gttttgctgt taaacgaata
    22741 aggataacta aaatgacaga agcgatgaag attaccctct ctacccaacc tgccgacgcg
    22801 cgctggggag aaaaagcaac ttacagcatt aataatgacg gcattaccct gcatttgaac
    22861 ggggcagacg atctggggct gatccagcgt gcggcccgca agattgacgg tctgggcatc
    22921 aagcatgttc agttaagcgg tgaaggctgg gatgcggatc gctgctgggc attctggcaa
    22981 ggttataaag gcccgaaagg cacgcgtaaa gtggagtggc cggatctgga cgatgcccag
    23041 cgccaggaac tggataaccg cctgatgatc atcgactggg tgcgtgacac catcaacgca
    23101 ccggcagaag aattgggacc atcgcaactg gcacagcgtg ctgttgatct gatcagcaac
    23161 gtcgcgggcg atcgtgtgac ttatcggatc accaaaggcg aagatctgcg tgagcaaggt
    23221 tatatggggc tgcacactgt cgggcgcggt tcagaacgtt ctccggtatt gctggcgctg
    23281 gattacaacc caactggcga taaagaagca ccggtgtacg cgtgcctggt aggtaaaggt
    23341 atcacttttg actccggcgg ctacagcatc aaacagactg cgtttatgga ctcgatgaag
    23401 tcggacatgg gcggcgcggc aacggttacc ggggcgctgg catttgccat tatgcgcgga
    23461 ctgaacaagc gcgtgaagct gttcctctgc tgtgcggata acctgattag cggcaatgcg
    23521 ttcaagctgg gcgatatcat cacctatcgc aacggtaaaa aagttgaagt gatgaacact
    23581 gatgccgaag ggcgtctggt gcttgccgat ggtctgattg atgccagtgc gcagaaaccg
    23641 gaaatgatca ttgatgcggc gaccctcacc ggggcggcga aaactgcgct gggtaatgat
    23701 tatcacgcgc tgttcagttt tgacgatgcg cttgccggtc gtttactggc gagtgcctca
    23761 caagagaacg aaccattctg gcgtctgccg ctggcggaat tccaccgcag ccagctgccg
    23821 tctaactttg ccgaactgaa caataccgga agcgcggcgt atccggcagg cgcgagcacg
    23881 gcggcgggct tcctgtcgca ctttgttgag aactatcagc aaggctggct gcatatcgac
    23941 tgctcggcga cttaccgtaa agcgccggtt gaacagtggt ctgcgggcgc tacgggactt
    24001 ggtgtgcgca cgatagctaa tctgttaacg gcgtaaacta atatgccgga tgcggcgtaa
    24061 acgccttatc cggcctacaa attctgcaaa ttcaacaaat tgcgaatccc ttgtaggcct
    24121 gataagcgta gcgcatcagg catttgcgcc ccgaacgttg aatgaattgt ggttcttatg
    24181 tccgaaacaa aaaacgaact tgaagacctg ctggaaaaag cagcaaccga accggcgcac
    24241 cgcccggcct ttttccgtac tctactggaa tccaccgtct gggtgcctgg tacggcggcg
    24301 cagggcgagg ctgtggttga agatagtgcg cttgatttac agcactggga aaaagaagac
    24361 ggcaccagcg tcattccttt tttcacctcg ttagaagcgc ttcaacaggc ggttgaagac
    24421 gaacaggcat ttgtcgtaat gcccgttcgc acattgtttg agatgaccct tggcgaaacg
    24481 ctcttcctta atgccaaact gccgaccggt aaagaattta tgccgcgtga aatcagtttg
    24541 ttgattggtg aagagggaaa tccgctgagc agccaggaaa tcctggaagg cggtgaatcg
    24601 ctgatattat cggaagtcgc agagccgcca gcacaaatga ttgattcact caccacctta
    24661 tttaaaacca ttaagccggt gaagcgtgct tttatttgtt caattaaaga gaacgaagag
    24721 gcacagccta atttacttat tggcattgaa gccgatggtg atatcgaaga aattattcag
    24781 gcgacgggaa gtgtagcgac cgatacatta cctggcgatg aaccaatcga tatttgtcag
    24841 gtgaaaaaag gggaaaaagg aattagccac tttattaccg aacatattgc gccattctat
    24901 gaacgtcgct ggggtggttt tttgcgtgac tttaaacaaa accggataat ctaaaatatg
    24961 ccgccctaaa gggcggcatt cgggcttact tgctgctctg aaggttgtta accaccagta
    25021 aattgtcgcc ggagagactc cggttattat ttacttggga aaacagcgca ccagaaacaa
    25081 taagtacgat acagaagcac cgtatcttca ttgtgcggtc catgcgaaga ccgcatcacg
    25141 acagcaccac tggcgatatt gccgcgatag caagcgctga gacgcgcttg ctctaacggt
    25201 cgcggaacat cctttcctta agccagcgcc cgcttgcgag acggcggaaa gggccgcagt
    25261 ggataataca ctaacagaac aataataact ctcttttgct tgacaaaaag agagttactg
    25321 gcgagtattg tctccgctga gacgcgcttg cattactacg gtaatacaac ctgctggaaa
    25381 ggccgcgaac cggaccagca acaaataacc gcctgatttt ggcggttttt tgttttttaa
    25441 aggaaagtaa atttacatgt ttaagtaata tcttttaccg gttagaattt ttaattttga
    25501 gcaggatttg tttatatttg tctgaattcg gactaataag ccaggttatt ttattaccga
    25561 acacggcgga aagggccggt gtcgataata cgctaaataa acaataatta ctctcttttg
    25621 cttgacaaaa agagagttac tggtgagtat tgttttgctg tgaagcacct gcgttgctca
    25681 tgtaacacaa gtcaacctgc tggaaaggcc gcgaaccaga ccagcaataa aaaaacgcca
    25741 aattcggcgg ttttttattg cctgtaatat acttatttca ctggctcaac cggtaaatct
    25801 gcccgcgcgc cccattcact ccatgcgccg tcgtacagtt tcacgtttgg cacatccagc
    25861 gtcgcgagtg ctaacaaaac cacggctgcc gttacaccag agccgcagct gacgataatc
    25921 ggtttgtcat agctgacgcc gcgaccaaaa aatatcgcat ccagttcatc ggtcgttttt
    25981 agttcgcctt cgcgcaccag ttccgtccac ggaacgttca atgcaccggg aatatgtccg
    26041 cgacgtaaac ctgggcgagg ttcatcaact tctgcgttaa aacgtgcagc cgggcgggca
    26101 tcaataattt gcgccgtatt ttcatggctt gccaataaca catcggttac tttcaccact
    26161 gcttcaggat taaacgcggc gttaaactct ccttccggca gctctactgc accttcttct
    26221 aatagcaggt catcgcgctg ccagcctgca agtccccgtt actttattcg taccccttat
    26281 aatggggtgt tagccagcca gacccggcat gattactgcc cccagtcgtc catgatccgg
    26341 ggggggatgt caccgggtct ggtggggcgc tggtaaccgc taataggggt caggtcaggc
    26401 acttttgccg ggaccgtctg taacgt
//
LOCUS       DAZXUK010000028        25663 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZXUK010000028 DAZXUK010000000
VERSION     DAZXUK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25663
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.028"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(218..949)
                     /gene="traT"
                     /locus_tag="ACH3L8_000973"
     CDS             complement(218..949)
                     /gene="traT"
                     /locus_tag="ACH3L8_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFT4084191.1"
                     /translation="MKTKKMMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDAAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(963..1472)
                     /gene="traS"
                     /locus_tag="ACH3L8_000974"
     CDS             complement(963..1472)
                     /gene="traS"
                     /locus_tag="ACH3L8_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFT4084192.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITLFVIISFIYTIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            complement(1469..4294)
                     /gene="traG"
                     /locus_tag="ACH3L8_000975"
     CDS             complement(1469..4294)
                     /gene="traG"
                     /locus_tag="ACH3L8_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFT4084193.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAERRRAMAWFFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            complement(4291..5664)
                     /gene="traH"
                     /locus_tag="ACH3L8_000976"
     CDS             complement(4291..5664)
                     /gene="traH"
                     /locus_tag="ACH3L8_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFT4084194.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMNYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            complement(5651..6043)
                     /gene="trbF"
                     /locus_tag="ACH3L8_000977"
     CDS             complement(5651..6043)
                     /gene="trbF"
                     /locus_tag="ACH3L8_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFT4084195.1"
                     /translation="MKEKINDTEPGIKQIEREIERGCDNAKKYFWLFVVFFAAGLIVR
                     NVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLAAASLSPLCEII
                     FGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            complement(6024..6371)
                     /gene="trbJ"
                     /locus_tag="ACH3L8_000978"
     CDS             complement(6024..6371)
                     /gene="trbJ"
                     /locus_tag="ACH3L8_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFT4084196.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            complement(6301..6846)
                     /gene="trbB"
                     /locus_tag="ACH3L8_000979"
     CDS             complement(6301..6846)
                     /gene="trbB"
                     /locus_tag="ACH3L8_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFT4084197.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            complement(6833..7117)
                     /gene="traQ"
                     /locus_tag="ACH3L8_000980"
     CDS             complement(6833..7117)
                     /gene="traQ"
                     /locus_tag="ACH3L8_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFT4084198.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            7198..7533
                     /locus_tag="ACH3L8_000981"
     CDS             7198..7533
                     /locus_tag="ACH3L8_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFT4084199.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYIYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            complement(7514..7855)
                     /gene="trbA"
                     /locus_tag="ACH3L8_000982"
     CDS             complement(7514..7855)
                     /gene="trbA"
                     /locus_tag="ACH3L8_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFT4084200.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(7869..8612)
                     /gene="traF"
                     /locus_tag="ACH3L8_000983"
     CDS             complement(7869..8612)
                     /gene="traF"
                     /locus_tag="ACH3L8_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFT4084201.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(8605..8862)
                     /locus_tag="ACH3L8_000984"
     CDS             complement(8605..8862)
                     /locus_tag="ACH3L8_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFT4084202.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGIL
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(8889..10793)
                     /gene="traN"
                     /locus_tag="ACH3L8_000985"
     CDS             complement(8889..10793)
                     /gene="traN"
                     /locus_tag="ACH3L8_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFT4084203.1"
                     /translation="MKEKINTFFRSLNFNYSVKPCNLCAIVFFLSFSVFADSNSDYRA
                     GSDFAHQIKGQGSSSIQGFKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWA
                     TGETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEF
                     TNYTCERDTMVEEYCTRTASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPV
                     TGTVESASLSVYAAFFFLNSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLT
                     GSGCTANGNCLPHGNGDRKVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     ADGVLTGTECSEPGGTKTGVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLASRQCAFYSDEGTCLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLA
                     KCNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKHPDCRGITVDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQ
                     IADQLKQAGQ"
     gene            complement(11029..11409)
                     /locus_tag="ACH3L8_000986"
     CDS             complement(11029..11409)
                     /locus_tag="ACH3L8_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084204.1"
                     /translation="MTLPAGYYQIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLAWGWSVKGAFNSKFQLCFRLQPDTPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            complement(11406..12044)
                     /gene="trbC"
                     /locus_tag="ACH3L8_000987"
     CDS             complement(11406..12044)
                     /gene="trbC"
                     /locus_tag="ACH3L8_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFT4084205.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVARALLNNPGDKQK"
     gene            complement(12053..12358)
                     /locus_tag="ACH3L8_000988"
     CDS             complement(12053..12358)
                     /locus_tag="ACH3L8_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084206.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(12388..13380)
                     /gene="traU"
                     /locus_tag="ACH3L8_000989"
     CDS             complement(12388..13380)
                     /gene="traU"
                     /locus_tag="ACH3L8_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFT4084207.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(13377..14009)
                     /gene="traW"
                     /locus_tag="ACH3L8_000990"
     CDS             complement(13377..14009)
                     /gene="traW"
                     /locus_tag="ACH3L8_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFT4084208.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(14006..14392)
                     /gene="trbI"
                     /locus_tag="ACH3L8_000991"
     CDS             complement(14006..14392)
                     /gene="trbI"
                     /locus_tag="ACH3L8_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFT4084209.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(14389..17019)
                     /gene="traC"
                     /locus_tag="ACH3L8_000992"
     CDS             complement(14389..17019)
                     /gene="traC"
                     /locus_tag="ACH3L8_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFT4084210.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(17145..17492)
                     /locus_tag="ACH3L8_000993"
     CDS             complement(17145..17492)
                     /locus_tag="ACH3L8_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084211.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(17520..17738)
                     /locus_tag="ACH3L8_000994"
     CDS             complement(17520..17738)
                     /locus_tag="ACH3L8_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084212.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPFNSVMETLNGGGQ"
     gene            complement(17837..18310)
                     /locus_tag="ACH3L8_000995"
     CDS             complement(17837..18310)
                     /locus_tag="ACH3L8_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084213.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKVC"
     gene            complement(18303..18716)
                     /locus_tag="ACH3L8_000996"
     CDS             complement(18303..18716)
                     /locus_tag="ACH3L8_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084214.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(18709..18930)
                     /gene="traR"
                     /locus_tag="ACH3L8_000997"
     CDS             complement(18709..18930)
                     /gene="traR"
                     /locus_tag="ACH3L8_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFT4084215.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(19065..19580)
                     /gene="traV"
                     /locus_tag="ACH3L8_000998"
     CDS             complement(19065..19580)
                     /gene="traV"
                     /locus_tag="ACH3L8_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFT4084216.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(19577..19897)
                     /locus_tag="ACH3L8_000999"
     CDS             complement(19577..19897)
                     /locus_tag="ACH3L8_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFT4084217.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(19884..20474)
                     /gene="traP"
                     /locus_tag="ACH3L8_001000"
     CDS             complement(19884..20474)
                     /gene="traP"
                     /locus_tag="ACH3L8_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFT4084218.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(20464..21891)
                     /gene="traB"
                     /locus_tag="ACH3L8_001001"
     CDS             complement(20464..21891)
                     /gene="traB"
                     /locus_tag="ACH3L8_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFT4084219.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGTSKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(21891..22619)
                     /gene="traK"
                     /locus_tag="ACH3L8_001002"
     CDS             complement(21891..22619)
                     /gene="traK"
                     /locus_tag="ACH3L8_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFT4084220.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(22606..23172)
                     /gene="traE"
                     /locus_tag="ACH3L8_001003"
     CDS             complement(22606..23172)
                     /gene="traE"
                     /locus_tag="ACH3L8_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFT4084221.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(23194..23505)
                     /gene="traL"
                     /locus_tag="ACH3L8_001004"
     CDS             complement(23194..23505)
                     /gene="traL"
                     /locus_tag="ACH3L8_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFT4084222.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(23520..23879)
                     /gene="traA"
                     /locus_tag="ACH3L8_001005"
     CDS             complement(23520..23879)
                     /gene="traA"
                     /locus_tag="ACH3L8_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFT4084223.1"
                     /translation="MNTVLSVQGASAPVENKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(23913..24140)
                     /gene="traY"
                     /locus_tag="ACH3L8_001006"
     CDS             complement(23913..24140)
                     /gene="traY"
                     /locus_tag="ACH3L8_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFT4084224.1"
                     /translation="MIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVIEENESTFKEL"
     gene            complement(24260..24907)
                     /locus_tag="ACH3L8_001007"
     CDS             complement(24260..24907)
                     /locus_tag="ACH3L8_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TraJ family protein"
                     /protein_id="HFT4084225.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFSCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
     gene            complement(25099..25482)
                     /gene="traM"
                     /locus_tag="ACH3L8_001008"
     CDS             complement(25099..25482)
                     /gene="traM"
                     /locus_tag="ACH3L8_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFT4084226.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
BASE COUNT         6422 a         6883 c         5772 g         6586 t
ORIGIN      
        1 attgtctgat gtcataatga ctttctccgg aagatcccgg cttcgccggc aacacttcac
       61 ggtcgcacaa agaatttgca caaccgctca gtgttttcat atttatttcg gaaacgccgg
      121 gaggtgacaa aatttatttt gttacatccc ggggttggac aacgagcaac gcgaggcgtc
      181 agcaaggagg ccggtcagat cagcctcctg acgaggttca gagaatattt gcgattgatt
      241 tggccagctg gtcttcgaga acaggcttcg cctcttcaaa tttcaggtta accttgttcg
      301 catttgagac cacacgggtc tggtatttat gctggttacc tgtttcagta ctggtctgaa
      361 ttttcgcacc ggatgtgccc tgacgcaggg cggcaacatt atccgttgtc accgttgcct
      421 tagtacgctc tgcaatctgc acatccgtga tcatggtata gttcacatct tccaccatcg
      481 catctgcagc catacccacc agaccagcag caaggcccac accgagtgtg gcaccggcag
      541 aatttgagtt atagccggta ataccggcac ctaacgctgc accaacagct gcgccttcat
      601 aaccacggtt cagccatccc tgagactccc gcagatccat cttatcggcc ttcagcacat
      661 tcgcctgaat ccagtagtag gctgcatccg gagaagtcac cacctgatat ccttttgctt
      721 tcacagcatc agcaattttg ccctgcagcc cactcatgtc tttatcagac gtgtttttga
      781 tctgcagaaa taccgtgcgt tcgctggcgg gtttaagcca gatggtctca ctcatctgag
      841 tcttcacctc aaggttacgc ttcttgattg ctgtgctcat cgcaccacac cctgaaaggg
      901 ccagagtgga actgaccagt gcaaccatca tcattttttt tgttttcatc cagaatatcc
      961 ttttaactaa cagactcttt tttgaacaga cctattactg agactattgc agagagatta
     1021 taacgattta tatctatagt ataaataaag gaaataatta caaacagagt tattgataaa
     1081 aacccaaata agaacgtatg agcaaaggaa actattgaaa aaacaatcac aaacgacaag
     1141 atataagttt tgttcttatc atacataaaa gatataactt tcgactcatc tctgaatttt
     1201 ttagggatgg caagatacaa catcattcca ttagtaatgg caacaaaaag gataaaaccc
     1261 attacgccac taaaacatat atcaaatcca gcatctcgga tatcaacttt atctgccagc
     1321 aaacgaagaa atgtcggcca taataaaacc cataaaaaca catacacccc tggttttatg
     1381 acttgtaata atggcggaat attaactccc tctacagaga gtacatcctt tagtttctgt
     1441 gtctccgttt ttatatcata gtgagtgatc ataatggccc cttcagtcta tcctttttat
     1501 catcaaccat ttttttcaca gcctcttctg atggctcagg tattatcttt tgactttctc
     1561 gctcttcatt atatttatta ttctgagata atgcctctgt tttatgatga ttttgcagtt
     1621 cagaatactg ccctcttaca atattttcct cgccacggat actgttctga gtatctccga
     1681 tattgccacg atattctgtg accatattat caacctgatg ttttacatca ttacggatat
     1741 tactgtcctg cgttctttgc tcaataatgg cctgatgtcc ctgatggtca gcaataatat
     1801 cctgactgcc accacccgaa ggtacgctct ccattccttt accaatatcg ccacgggatt
     1861 cccgccaggc attatcaaca ccaggctgta cctgttcctg cacaaaaaac caggccatcg
     1921 cccgacgacg ttctgcaatc tccggcgaac tggtatttgt cagaatagct tctgcatcct
     1981 gcggtgcgcg tttcatcaca tactgtgcaa actgttgcga caggtcttca ctcatctgcc
     2041 cgctcatgct ttcagtacgg gaggccattt ccgcatattc atggctgcgg gtcatattcg
     2101 tcgtgtactg gtcgtaactc tgtttcgctg agttcagggc tgcagacaac tggtccaccc
     2161 gggagtcagc attattgtca gtatggctgc cggactcact gaccttgcga ctggtaaagt
     2221 aatcgctggc ttctttaaag tccttactgg ctttagcgtc aatatcatgg cgagcatcat
     2281 gggtagctct gctgccacta cttgcagaat gtgaatcatc atccgttcga tcaagacttc
     2341 ccttaaaccc gcctctgaca ccaaccccac caccaacacc aagaattcca ggtttaatcc
     2401 cccattcagc atgagcatct gcatatccgc catacgagaa cttagaacct gttgatgcca
     2461 gttcctgagt cgcctgctca ttactgatat tatgcgcctt cgcatagctt tccactgcac
     2521 tgcgcatacg gctggccatc atggagtcct gtgcactcat ggtgctgtca gcgccaccgg
     2581 tgacagaatc actgctcccc cggttagaac caaactgact gagcgtgttc caggcactgg
     2641 caatactgct gctgaaacca tgcagggcac tttctgctct gttcgacgcc tcccgggcca
     2701 tttcctgttg cgccgctgca atctgacgcg ttgcattgat accgaccggt aaacgggaca
     2761 tcgctccgct tgcatccatc accatattac cgtcacgggt ctgtgtggca gttgcgccac
     2821 tgccggtctg gtacatcatc tgaccaaacg acgtggtgct gttggtgctc cagctgaagc
     2881 cgttcacgtt ttccgtctgc atgttgccgt aggagtaatt accgtcaacc acactgcctg
     2941 ctgcactggc cgtcggactg atagaggaag aggcgaagtg gctgtacacg ctggaaaaac
     3001 ctgcgccaag gccttttacc atcatccatg acaacggggg gatcatggct gaaagatacc
     3061 cggcagtaga ggccaggtca gagtatttca gctgtatctg agagagttca gacagcacga
     3121 ccggcgcacc attcatcttc gcatagaatg tcatggcact gttcagaatg gcatacaaca
     3181 acggccaggt ctgcagccac atcagggcaa acacatagcc tttcaggacg gacagtgtca
     3241 gtttgttgaa gacagctgcc agtaccagaa gcgggaaaat accaatggca attcccgtca
     3301 ggatagtctg taccattggc aggtttcgca ttgtcacatg accgatagag acatgcgcca
     3361 gacgttgctt ctccatcgat gacgtggtgg ccagattcac caggctggcg gtgtcaccat
     3421 tacgggcagc attactggtg ataccctctt tcagggcatt tattgtgacg ttctggcgca
     3481 taatctggct ggcagactga ctggagccgt agaaataact gtaactgtca ctgaccagtt
     3541 gtctgaataa caggtccggg tccggtctgc cgccaaatat ctgctgcaca taataatgcc
     3601 aggtcttgcc tcccgtcttt gtatcgagat tcagcctgtc tttcagcgtg accgacgcat
     3661 ccttacaggt tatgaaatta ttattgttgt cataaacgcc ccgtagcggg ctgggtcggg
     3721 aaaaaatcag cgtgtagggg tcagcggagg ccatcagatc ttccagcgtg tatttatgat
     3781 tcaggtaaat atcacccagc acgcagttct ggacgtagtc ctggaacaga ttgatgattt
     3841 ccggattccg ggacaggaaa tcggtgcttt tcacaatcag attcgccccg aacagcatgc
     3901 ctgtcttgct gtaggtgacg ctgtccggtt gggtgaatat catctcgtag ctgaccacca
     3961 tcgcatgccc gatacgggtc gtaagagaaa gtggcatcgc cagaccgacc ggaacattat
     4021 ccacccgatg gactttgacc aggtcactgt tatcaataat ctgcaccgat gtgcggacat
     4081 taaccagcag gctgataagc acaaacacgg ccacccagcc cagcaaatcc atcacgttat
     4141 gccgctgtac ccacataaaa gccacagcaa caacagacag ggtgagcgcg attttttcaa
     4201 ttgaatccca ggtccgggta ctcataaatg cagcaatggc gttcaggtta ttccgcaacc
     4261 actcaccacc ggcaatcaca taaacttcat tcacagtgaa ttccctccga agtgatagtt
     4321 gttctggtaa cggctgagca tacgggcgga aagctgctga cgcatgtagt tcatctgacg
     4381 ttcgacaacc agcagcgcat cctgctggac ctgtacctga ctctggaaaa tggcaatctg
     4441 ttgctgtgcc tgattcagat tctccagaac attgtccatc acagcctgcg gatagttccc
     4501 ggtggcaatc atggcccggg cctgctgaag cagttcctga atgtactgga gcatgatgtc
     4561 gtagccgata tagtccgtca gctggtaaac gacgctgttg gacacaccga gcatctgcgg
     4621 gtcaatcaga tatttaaaaa cgggaatggt ggtgctggaa ataaacccct gttcccgggc
     4681 gtcaagcgga gtgtcactca ctgctttatt ctgaatactg gtgagcagtt ttgtaatctg
     4741 tgacttcagg gctttatccc gggcgatggt cacatttgca tccgccacca ccttcagaca
     4801 cttgtccgaa tcgttgcagt ggtaaatttt tgccgttccc ccctccataa tcgcccggag
     4861 gatatcgcgg tcagtggtac gcgccggcag gggggtaatc tcgctgtctt ccccaaaaat
     4921 cagcgtaccg gtgagcgtca tgataaattc tttcaactct ttgttgccgt caaacattct
     4981 gttttttttc agcgcctccc atatgatatt ctggtttttc agcacccgct ccttgtcctt
     5041 gtcgctggct ttgtcacgaa cagatgagga ctgccctccg accgtgcacc cctgacgaga
     5101 tgcggcccag tcggcgaaga tattgctttc actgccaatg tcctgacaaa ctttctgctg
     5161 agattcctgc gtctgaggga acagcccccc cacaagcccc tgtgccacct gacaggaact
     5221 gaggttcata ctgttaatgt cacttgccat tttctgcagg aagtcttttg cggtttttat
     5281 ttccggcacc gttgtctgca gggcaaggtc aaaaaagtaa ccggcagcat tgctcataat
     5341 ctgcttaaca aaacgctgca actgatcgcc attaataaaa ctgaacgagc cgagataggc
     5401 gtcgatgccg ccacatccgg cattgatatc cggcagcgtc atggaaatca gctggatatt
     5461 tttgacctgg gtgcgggcat acagggagcc accataggca tacccggcgg cctgcccctg
     5521 ccagacgccc ggctgtgtgg tattggaggc gaagcccagc ttgttaaaga actgattcat
     5581 atcgctgttc acatccgctg acgctgcggg tgtgaccgtc agtaaagcgg cacaaagaac
     5641 cagaagaggt ttaatgcgaa gcattattgc tgccctccct taatgtgttc tcccggcgca
     5701 tacggcgttt cagcaggaat atccggacac ccccaaatat tatttcgcac aacggcgaca
     5761 gagaagccgc agcaagaaaa ccagctgcaa gcgcatataa cataatcgga atgacataca
     5821 tcagggtgtt ccacatataa cgaaaattat ttaattccgg gtcagctttc cacgaatcaa
     5881 taccggcact gaaaaaatca tgcataacgt tccttacaat caatccggca gcaaagaaaa
     5941 ccacaaaaag ccagaagtat ttttttgcat tatcacaacc acgttcaatc tccctttcaa
     6001 tttgtttgat tcccggttct gtatcattta ttttttcttt catcataagt tccctctgat
     6061 atttctgatg attcatgcag acaggtcatg cggctgtaat atatttcaga caccgaaatc
     6121 catcccacaa ttgcccccac aaaaaacaca acagaaacag aaaaggtaag acacagcgaa
     6181 acgacaattt ccacagggct ggcgggataa aaaagcccct gacggataaa ggggatattc
     6241 aggctgacga ttattcccgt cgcgatgccg gatattccgg caatgagtaa caccacctgc
     6301 ttatttcgca cctttttcct ctccgtatat ctgcagaacg gtatccatcc gcgccataaa
     6361 actggcggca tccgttgccc cctgcaaaag cggtaatgcc tcaagtgtgt tgacgttgac
     6421 caggaaggtg gtcggtgtgg ccaccgggat attcgggaag aaggtctgca tcacgtccgg
     6481 tggcaccggt aatgcttccg gaaaggcagt atccccctga ccatccaggg tgtagggaaa
     6541 aacggaaaag ccgtactgct gtgccagctg cttcagtacc gggtcaaact ggtgacagta
     6601 agggcaatgc ccctgcataa acagcaccac cttccagtcg gccaggttaa cctgcctgcc
     6661 gttgctgagg cggaaccagc ggggagccgg cttttctgtt ctggctgatg tgtctgctgc
     6721 cggttgtgca ggctgcacgg ccattccctg cggattccag agtcgttcaa tttcatcccg
     6781 ggtggaggcc tgcacacctg tggccatcat caacaaaatg accggcagta atttagtgag
     6841 agacatgtcc gccctcctgc tgcccttcca tcatggcctg ttgcctggct tccagtgcct
     6901 cacgctcttc caggctgaca cgtgcaatcc atgcatccag cacctggtat gcgccgaaca
     6961 gcacaaggat ggccgcaatc aactccgcca gaaaaaaagc catccagggt ttgctgtaaa
     7021 caagacgggc gacgatatga aaccagacac ccagggaaaa tacccacatt cccgtaatat
     7081 caagccgggg taaagagaat ctgcgtttac ttatcatgtt aaactccggt tattatttac
     7141 atgagagtat tcatgtctgt aatattcgcg tttgtgtgat attaatcaga ggtacaaatg
     7201 aaaaagttat cttttaaaga aaaactggaa atagtgagaa acatcataag agagtcatta
     7261 tcaggtaatg cggcttttgc tttactggtg tattcattat tgcactccct ccccattaaa
     7321 acctttgctg atttatttgt gacgatactg ttatttatag cgattatcgc tgttgtgata
     7381 tggctatttc taatcatcta tatttatttt tgtgagttat tcagaagtca ctggatagca
     7441 gtctggttta tcctctggtc tgtggtaatc aacctcacta ttggatttgg catttatgac
     7501 aaattagcac cagtcatttc actaatgaaa taataaaaaa ccacatcact ccagaccata
     7561 tggctgacaa cataatacaa acaaacatcc ttctgaaact gtcaaacttc gtgataaccg
     7621 gtatggtact gactgccatt gaaacaaccg gtacggcaca ctgattcata accgcaccac
     7681 aaagaaacaa gaaattatct ggcagcatac atacacttat gacatttact acaaagaaga
     7741 caaacgagct cagcagataa actcctttat tatttggtcc tgtaaagaaa taaccaacaa
     7801 cccccacgat aaacaggctg gcacctgtaa aaaaacacaa atcccctgta tgcatatata
     7861 acctttggtt aaaaattcgg tttaaaatct tcagaaacgt tcaggaactg ttttgccagg
     7921 tcgtcctgtg aaataaagcc gtatgataac gggcgaacac tgccctgttt cgggtcaacc
     7981 agcatcatgg ccgggaaata tttcacaccg aggcgctgcg cctgcccctg gtcagtccgg
     8041 gaatccggca acagcggatt aatcacgcca tccacggaaa cgggaataac actcagacca
     8101 tacgtatccc ggaagccatt aatgacctgc gccagttgcc cgtcgatggg gtcctgcccc
     8161 cggtaaaaaa acatgatgcc gtagtgttca gccagtttcg caatggcctg tcgctgctgc
     8221 gcctggtctg ccgccagctg gttccgtacc gtgccgttgt aatggctgta ctgcaggtta
     8281 tagtccagtt cgggatgtgc cagcatggcc tttttagcac tcatggtgaa aagcccggcc
     8341 tgctgagtcc agtaattctg cagccggaaa tatttaacaa aattatccac gccgggatac
     8401 agaatggctt cgtacagcgc ccgctttgtt gccgtctgca gcgcggccag tttctgcata
     8461 atatccggct cctgacgggg ggctgcaggt acaggtttat tttctttttc cttcggattt
     8521 attttctcgt tataccactg ccagcctgca tctttccctg acgccggaaa aataaagcag
     8581 cagagtaaca gtggcagtaa tgctttattc atcttcattc ctttcatcat tttttctttc
     8641 atcatctttc tgcaggccag tcagggcaga aaaacaaaaa tacagaataa acaggagcac
     8701 accggagtaa aggataacga aagcagcagc aagtatcccg tctgccgtag tttcttttac
     8761 cgtatcccac gaccagataa ttaccggaga aatgacaatc gctgtaatcg aaagccggat
     8821 aagaaaatca acaagacgat tagaagtgaa tataactttc atcatttccc ccggttattt
     8881 tccatacctt actgtcctgc ctgtttcagc tggtcagcaa tctgctcttt cactttctgc
     8941 gtcagaacgc cactgtccgg gattttctgg ttattcatca ggtcttcgta gaagttagtg
     9001 aaatccagtc tgtcgaactg aattttctgc agctcatcaa ccgtaatccc ccgacagtca
     9061 ggatgcttcg cactgccaaa cccgatacgc aactgcccgt tacgcccctg ctgctggact
     9121 atctgcgcca gcttcgaatc aaactggcag tagctgcgct ttttctgcag acagacaccc
     9181 agcactttcc gggagcagaa ctcgcccaca ctcaccgtca gtttgttgtc ctttgccttt
     9241 cccagcgcct tttcttcact gttgcacttc gccagcccca catcctgacc ccagccgcca
     9301 tccttacagc agttactgaa gccggctgcg gccttacggc agaatttcgc ctcacccgtg
     9361 aaagccctga catccacacc attcagggcg gcaacatcct tacctgcggc ggcaagcgca
     9421 gccagctgtg atactgcttc accaaaatca ctgttttttc cgctctgtgc cttatcgcat
     9481 tcaccgtcaa ggcagaagac atcaccacca cagaccatca cttttcctga tgttctggac
     9541 tcgcaggagt acgtggcgta ctcatgcaga caggttccct catcggaata aaacgcgcac
     9601 tgacgtgagg ccagcgtaca ggccggatta tcgacatatt tctgacaggt gccgttatct
     9661 gcggactgag tgacatattt atcccggtat gcccagcatg cctgtgtgat gttccagggt
     9721 ttaccttcca tcactccggt tttcgtacca cccggctctg aacactcagt gcctgtcagc
     9781 acgccatccg ctttattaaa cggacaactc tccacccact ccacccgggg aacccattct
     9841 ttatcacgga ccttcatcct gagtttcagc gtaaaggtgg aagcaccact gacaagcgat
     9901 tcatagactt tcctgtcacc attcccgtgc gggaggcaat taccgttagc agtacagcca
     9961 ctaccggtca gaacctgccc ctgtgtcacc tgcaaacctg tcgccccgga cagggggtaa
    10021 gtatccgact gcccgtttgc caggccgaca ttaaaggttg tattcatgaa ggtataacgg
    10081 gagttcagga agaaaaaagc ggcgtataca gacaatgatg cactttccac tgtaccggtt
    10141 accggagccg tgacactgaa caccagtttc agcccgttca tactgaaccg gaactgtgag
    10201 tgcggtatcg tcacttcacg atattcatcc gtgtatttcc agtccccggt gatactggca
    10261 gtccgtgtgc agtactcctc caccatcgtg tcccgctcac aggtatagtt tgtgaattca
    10321 ctgcggttta tctcctgcgc actgcactgc tgtccggtat ttcccacaat gctgtcagcc
    10381 ctgttcacca catccctgcc ggtctgaatg aacggtgcat ccggtgacag aatgtctttc
    10441 ggcttgttca tgaaggactc tgtgatggtt tttccggttt caccggtcgc ccattccgtg
    10501 gtgccgtcat ttttcaggcc gccatccccg ccggcagtca cgccaccgta atattttgtc
    10561 tcgtccggat tcgcgttata gccggggata ctctcctgtg gcttaaagcc ctgaatactg
    10621 ctgcttccct gccctttgat ctggtgagca aaatcagaac cggcccggta atcactgttg
    10681 ctgtcagcaa acacagaaaa gctcagaaaa aaaacaattg cacaaagatt gcacggcttt
    10741 acggaataat taaaattcag agatctgaaa aaggtattta tcttttcttt cataacctga
    10801 accatactgg agaatgaagt gatgtcatct caacttcaga actgtgttga ttgtggtaaa
    10861 gcattaagtc gaacagccac aacgtgcggg aattgcagct caacggaccc ttttggaaaa
    10921 aaacggcgca gcgatcgctt ttaccagtgg tttgtagggc tgtttattac ggctattatt
    10981 attgctggcg gattatggta catggggctt cttgacgtac cgggattact actcagataa
    11041 cgacttcagt aacgatatca gtgttctgaa atcggcacat aacgagtgac gacaataccg
    11101 gttataccag taatgagggg tgtcgggctg aagcctgaaa cagagctgaa acttactgtt
    11161 aaaagccccc ttcaccgacc atccccatgc cagacgtggt attgctgatt cagcatcctg
    11221 ccgcagtcgc tgctcaagaa gggcagccat ttttaccgga caggcaaagg caatataagg
    11281 aggcagcttt gtaacaggga aaccatgtcc ctgacaggaa gcatatgtgc ggaagccgtg
    11341 aatgttcatc gcggccacca gggcacggat ctccggatca atctgataat acccggcagg
    11401 taaagtcatt tttgtttatc tcccgggtta ttcagcaacg cccgagccac ctgccggcag
    11461 tcccccgtgg cggccacttt ctccagcgcc tggccgaccc gcaggttacc tcggataatg
    11521 tcgtatccct gactgcagaa caccaccagt gccggtacac tgcgaatgcc gtactgtgaa
    11581 aacagcgtcg ggtcgatctg aacaccatca gtcgcgccgt ctttcaccag ggacagcacg
    11641 gcttcagcgg tggtcttcag gtcattattc accatgcccc gcagtgtggc gggtataccg
    11701 tagtgccggg tttcacccag catccgtttc agcccctctt cgggaatgga aaacgacaca
    11761 aaatacaggg caccctgccg gggtttcccg tcctgcgaag cctgctgttt acgaaccagc
    11821 tcatccagaa aatggttatc tgaacgctga agggggtttt ccagcacctg tttctccgcc
    11881 caggctttca gctgatggtc aggtttctct cgcagttgtc tgcttaaatt ttcctgctgc
    11941 ttcaggaact ggcggttttc aggtgtgttc acattttctg aagcatgaac agcaagatgc
    12001 atccctgctg tcagagcaaa aagtgcaaat attttccggt tcatgatatt tctcactttc
    12061 tgctatttac aggcatgaac tcatcagaaa aaagataatt gagaaaaaca gccataaacg
    12121 cacatgtaaa tgtcaccttc agatataatc tgacaagtgt cacttgttct gatatatctg
    12181 ccttcttcca ttcatgaata atttcagcga gaaaactgtc aggtgcaaaa taaaataata
    12241 taacgggcac cattacaaga ccaaaaggaa ataattttaa tgttctgctt atcagcctga
    12301 gcagcacagc acttattttc ctgaggtgct gaatatattt actgtccatt gttgccattt
    12361 tcatttcctc ttattcatta aaatgattta caggaataca cagttacgtt tacgccacat
    12421 caggtagccg aagtttttct ttgtgttggg cggatttttt cccgtctccc agcgcatcac
    12481 actgcgcccg aacggatggc actgcccgct gtccggatac atattcacca tctgataacg
    12541 ccagcgttct ttgggcagga ttggggacgg atattcatta cagacggcgt tatttttccc
    12601 gatggtttcc ataatcatgc cctgacggtg tagtttgaac gccatgcgtt cactgaccag
    12661 cagggaggac tgcaacggac tggactcatt actcacccag ccattgaacg ggtacatact
    12721 tccctgcgaa ccggcacacc agaacagaac atcgagaggc atattaaagg cgctggcaat
    12781 ggcatctgct gcacaggccc cctgtgctat cggattggca aagatgacag cttccggatt
    12841 gagaatggtg gtcaggctgc tgtccgtcca ggtggggtca atttcagaaa gataagcgat
    12901 atccaggtca ccaccttcca gacagcccag cgatgtgatg atgttcagcc agtacgtcag
    12961 cgggtatttg taccagtgaa cgtgatagaa cgccccgttc acctgcttct catccttttt
    13021 cgccgttccc tgtgccgttt taccaaaagc cggcaggctg aagcccaggt taaccatgca
    13081 tcccggtgac cgggtgacgt ccgtcagcgc catcggctcc cagtaaccaa tggccagccc
    13141 gatacgcctg aacagcggcg gcggtgccgg acaaatctga atgggcatcg acgggttcgc
    13201 cgtgtcgggg accttaccct gactgacttt gacactgccc agcgagagcg ggaaaataca
    13261 gctccagcag atatccgtga tcgggtttac aaaacgcccc tcacaggcag aatccgcaga
    13321 cgctgcaggg acatgaccgg cgagaaggaa taacatcagc agccacaggc ttcgcttcat
    13381 tttctgccct cctctgccgg aataaattcc accttcagga agcgatcgcc gggaacggcg
    13441 ctcacccgtg ccgggacctg atcaatgccg aggcgctggc agagcacacc gttctggtca
    13501 aagtaaacac ggctgtccag agacttctgc atctccggga tactgccctg cacgaggata
    13561 attttgctct ccagtgtggg cggcgtctgg cgtttcatcc aggccacctg cgccggatca
    13621 tcgccattga tgaaatacag cgtctggtta aaaggcacat actgcagcgg attcatcact
    13681 tcaccctgac gggcaaacac ccgcccttcg ttatcccgga tatccgcagc cagtctgacg
    13741 gacgggtcaa acaaccggct gccgtatttc tccgtgcgtc cgataccggg cacggcaggg
    13801 ggccgcagac tgttgcggat cacccgctcc ttaaaggcat ccattttccg gcccatctca
    13861 ccggactgct ccagtgccgt caggcgctgc atgatcaccg tcagcatgtc cggctccttt
    13921 accggccaca gatcgcccca ggtgccaaga tcggcggcgg ccacactctg cccccatatc
    13981 agcagggcaa tcagcccccg gcatctcatg gttccgctct cattcgccgg gcaatatcct
    14041 gctggatttc ccgggtgata tccggagccc cctgtaccac ggcaggcgac accagaatga
    14101 ccgcgtggtg tttctgctgc cacgcctgca ggctggcttc cagtgccgtg ttaaaacgcg
    14161 ctgaaagggc ttttgactgt gcttctgaca gttgtttctg actggcgctg tcaaaaaatg
    14221 catccacagt ctgtttcatg ttgaaggcgg cagtcaccgg tgcgttcagt ctgacaatgc
    14281 cgtaactgac ggctgcgttc agtaacacca ttgccaggca tcccggcacc gtccaccacc
    14341 agtggctgcg tcggggggct gtaacatcag tcgttttttg cgttgtggtc atgcaacact
    14401 cctgtatttc tcatgttctt ccagccaggc ttccagcgaa gccatctccg gccctgactt
    14461 cttccacgcc agctgccaca ccgcctcatg aatactcagg ccctccctgc gtttctgctg
    14521 cacaaactca aaatccgggc cgtcagagct gtacatggcg cggcttaacg ggtccacaaa
    14581 cagacggtgc caggaggaat ggttttccac ctgcagcagg aaggaactga accactggtc
    14641 tttggccgca ccaaacttgc caatcatgtc gcgctgcagt ggctggaact gatccgggaa
    14701 taactggtta tatttcgcga actccttcgc actctgtttg aggataattt tgtaggagga
    14761 gttaccccat gccgcacggg ccgcactgga ggccttgtca gagtcaaagt cgacgatgtt
    14821 ctgtgtgatg gtgatatagg caccggtatg gcggcgacag gtgcggtaac ctttttgaat
    14881 aaactcaccg accttgcgat ttttgaagtc cagcagacgc cagccttcat caatgacgtt
    14941 cagtttcttg agagtacgcg gcgtgcggta catcctgttc tcgatgtaga taatcaggga
    15001 gaacatcacc gcaaccagca gtgacggacg gtcttccagt ccgcccagct ccagcaccac
    15061 cattttcgca tcatcccgca gggacggctc atcggagtta aaatactggc cgtaagtgcc
    15121 gttggcagtg tactggtcaa gcagcacaat catttcgtcc agacggctgc ggatggtcgg
    15181 tgacccggca tactgctcgc tgtcactggc gtttttcagg aaatccacca cgtcatcaat
    15241 acgtgccctg ttctctttgg ccagccagga ggccctgacc gcctgcagca gcagaccttc
    15301 atgcacttca tccaggttac cgttggggct ggccatcact gacaactggt cacggacacg
    15361 ctccgctgac tggtcaatat cggtgatgtt cgcaaacggg ttaaaacgca gggtttcacc
    15421 gtccagatat acgcccccca tgttctcaca cagggacttg tagccatccc ccatgtcgaa
    15481 caccacggca aagcccccgg agtccagcac gctgcggata agtggctgta tcagtccggt
    15541 tttaccggcc ccggaggtgc cacagaccgc catgttgtag ttggtgttat tcatcccctt
    15601 aaagaagata tcgataaacg ccagctggtt acggtaggtg ggcgccagca gacctgccgg
    15661 tgtcaggggg ttatccgcca ctaacggcat caggttggcc acattaaagc tctctgcgcg
    15721 ctgaaccaca ccggcctctt tcagctgttt aaacagccct ttcccggcca taaagggcag
    15781 gcaggtcagg aaattgcgca tgtggttaaa gcgcggtgaa atcagctcaa aaccgttttt
    15841 acggaagctg ttcaggatgt cctgctccac ttccagtgcc gtttcattat tgtccttgca
    15901 gaaggctgtg atgttgagga agtaggacac gacagaagac tggccggagc ccagccgctg
    15961 gcgcagttcc ccccactcct tcgcctcttt ctccacggac ggaaaccatt tggcatatga
    16021 ggtcttcgac ttcttctcca ggtccatgta cttcaggttg gcttcgctgt gggttttcac
    16081 ctggtcttcc accaccagag tcagcgtcag gatgaacggg caggaaatgg acatttccgg
    16141 gttcagcaga ttgctgtagt tgtcggccat gttccacagg aaggcgattt ccgggttacg
    16201 ggccagatgg aaattcagga tgcgggccgt gctgttcctg ccgttctcac gcaggcccag
    16261 cgtcaggtaa tcagcccgga cattcaggtc aaaactgtcc tccacacact gataattcag
    16321 atcagaatac gggtccagct gacgtctttt cgggtacaga gagtccggat tatggttaat
    16381 catctccccg acaatctcga tgaaagcctg tgcatccact gtctgtgtgg tgatttttgc
    16441 gccgtgaaaa gaggcacgga tgattttcac cagattttcc atttccagaa tgtcagcccg
    16501 gctttttttc ttcgagggag aacagtacga gataaacacc cggtaatgac gcagggtcag
    16561 gggcagattc atcccctccg gcagcgggaa ctgtgtcgct gccgctttca tataataggc
    16621 acgggtaatg gcgttaaacc gttcagcctg ttcacctgac caggagaact cacgcagccc
    16681 gtattcaatc ctgtcaccaa ccagctgact ggacatcaga tggatacaca acggaatacc
    16741 gcgcggcagt ttggtgcgca gcatgtgatc gagtgcctcc acaatggact tattcgcccc
    16801 attgatggga atggcttcca gcataaagcc catcgtggtg tcattcatga acagaccgga
    16861 ttcctggttg taatcacggt acggcagcag acggctgaac tgcgggaagc tcatttcgcc
    16921 cagaacttca ttggcctttg cggattcgtc aggcagtttc agtgctgtga cgagggagtt
    16981 aaccgcctga gtgacggcct caagtgggtt attactcata tgagtattcc ttagtgcgtt
    17041 taaaaaagga aaatatcagt ctgtggccgt ggatatatca cgcagtgcca ttcctgaaac
    17101 cacagcacaa ataagagatg gaggctttta acaggaaacc aggattaacg ttcctttact
    17161 gaatgagcat catttaatgc ctgaatttgt ttttcgcgaa tcttattgcg ctcattttct
    17221 cttacccgtt tcacctctgc catttcgttt ctttttctgc attctgatgc gattttattt
    17281 gccttaccaa gtgttaaagg ttcagactga taattaagtg tctcctgaaa tgcttctgct
    17341 gcacagggag tttcctgtaa aatctctgtc agttgttctg gtgttgcagg ttgttgcgca
    17401 aggtaaacga tgtaaaaagg aatacataca atcatagcca taacgccaac aaatataaaa
    17461 gccagaacag attttaaata tatcgacttc atttcatttt ccttcgaagc agtaagcact
    17521 cattgcccac caccatttag tgtttccatc acgctgttga aaggttcatt tgttttaact
    17581 tcaatacact cccctgtatt tccaaagccc ctgccgccat gtgcttcgat tgtccgaaga
    17641 acctgcacgc atacgtccgt atttggcatg ggtgtaatat tccagcttcc gccaccgtta
    17701 atatttttta tcagcacaag aaaaataaag gtatgcattc tttattatct ccgcttgtat
    17761 attaatcact gaatatccct tcagttaact tatgaggtca taaaacactg cagattttca
    17821 ttcagattta ctctcttcag caaactttac tctcttcagc aaacttccgg atggtatcct
    17881 gttcacgctg acgggtggca gactcctgca ttgcatccac tttctcctgc cagagacgcc
    17941 ggatatcctt ttcatcccga ccagtcagtt ttttaccgga cagcagacgg ttaagcagtt
    18001 cctgtctcgc atccggtacg tcttcagtca gacgcataag ctgaagcagg ctctgacggc
    18061 tgagaaccat gagtgattcc ggcaggaggg tgaagaagaa aataaaaaag cataccgcac
    18121 cacagcaggc aaggaccgca aacaggattt tcagatatat accatcaacg ccgaacgcag
    18181 caataataca gggaatccat gcaatacacg cacaatacgc caggatgtta tctattcggt
    18241 tatgtctgcg atatatctgt tgatgttcat ccagaataga atctattttt tcagcttctg
    18301 atttatgcat agtgtttcct ctgcgtttcc tgccagagac gccggatatc ctgttcatcc
    18361 cgaccagtca gttttttacc ggacaacaaa cggttaagca gttcctgtct cacatctggt
    18421 acgtcttcag tcagacgcat aagccggagt aattcctgac gactgagtac catgcgtgat
    18481 tcctccggta cgctgtaata attaaaaata cgactgaata ccccacaaca gcaggcaacg
    18541 gacaggcaca gggatttcag atacaggcca gaaacacaga atgtggcaac accacaaaga
    18601 atagctataa ctaaaaatcc cttacccagt gccatacaca aagaaccctg ttttctggat
    18661 atccgctcat gctcatccag aataaaatct attttttcat tttctgattt atgcatagtg
    18721 tttcctctgc ctttcctgat acgcctgaca ttcaacgcac aacgtcacac cgggaaatat
    18781 tttccgccgg gcttccggaa taggatttcc gcatgcttca cagagataaa caggaatacc
    18841 atgaacattt atttcctggc gtatccggtt tattcctgtc atggtcagtt gttctgtcac
    18901 tgaatatgct tcatcggctt catcactcac ggaatattcc tcccgacatg ttctgacacg
    18961 ctaccgccca tcccttttca cgcacagggc actcgtttta caggcggatt ttgaatacag
    19021 aaagcccgcc ggcattgccg gctgatatac aacttcaggg ccagttaatt aatacgtggt
    19081 tttccccacg cagacggttt aataacaaaa aacacgctgg atggctgatg ataaacatcc
    19141 tggttatcaa tataaggcgc tatccataat gctgccgtct gctcacccgt tcttagcgga
    19201 cgaggaggtg ttaccggcgt aactgaactg accggaacaa ccgttttcac ttcccgggca
    19261 gcggtaaaca gcgggcgtgg tgccagcagt ttctgctccg gatgtgctgt caccgcaggt
    19321 ctgctgccgg acggagtggt cgcggtgacg gtctgcaccg gcattgtccg gaagttccct
    19381 tcagccaggc gcggcagtga tgccgcaacc ggctttgctt ctgaggactg ctccagtttt
    19441 ttggccttct cattggcctg ctccatcgtc atacaggtat cggatgtggt ggcgttacac
    19501 tcaaattccg tatttgttcc tgcgcagccg gaaagtaata aggttcccag cagaggaata
    19561 aataaagaaa tctgtttcat tgcccttctc cttttttatt actccccaca acagcagaaa
    19621 ttaactctgc tgtcagattt gttttccctg cacagggatg tccggagacc actgtatttc
    19681 ccggagaaga aaataagctc atcgacattc ccttcttcat cacaccgaca gcagccatat
    19741 taaatttgtg cggcaatgat tcagaactga acaccttcgg agcaggaaga actatttttg
    19801 tggtgcagtt actgagaaca gcatgcataa aatcatctga tacgttttta tccggcacaa
    19861 cgcgggctgt aataacatta atattacgca tgttcatttt tatctccgtt gcttctgttt
    19921 gttcggtcat cagggaaata aacatggcat ttcctgattt ttcccggcag ggaagcgtct
    19981 tccgtataaa cgtccttcag atattcatct gcggttacca cccggataca tttcattttt
    20041 ccgggcagca ccggatattt cccggtgatc gctgccagcg agacatacat cagtgccagt
    20101 gttgcccata atgcggtgac cagttgcatc agtgaccggt cagtctccct gacataatct
    20161 gcagcatcag ccgcgcggta atggcagata tcctgtattt ctgatctgcc tgaagaatca
    20221 gaggaagaaa accagcattc actgaccggg aactgtccgg caggtgctgt ttgtcttacg
    20281 aagttaatct ccttaaccag taatttcccc ggcgtggtgt tgtccttcca taaaacaaac
    20341 agagccatca gggccattgt ggccagtggc agaatgacgg tgtacttcag acaccagaac
    20401 agtcctcgca gaacccgggc gacaacatac cgggcggcat ggcacgcctg acgggacgac
    20461 atattatttg ccatcgttgc ctcctccctg gctgccggac ataaactgct gcggattcag
    20521 gttgcccagc tgcttcagca tctggtcagt attaaagccg ttaagatgac tttcagctga
    20581 tggcggaaca ggaaccggac tttccggatt atcttcttcc gctctgttct gcgtgcgctc
    20641 cagcgccatc tcttccacgg ttttcagctg gaagccgtcc tggaacacca cggtcacttc
    20701 gttgcccgca ccaatcggta tcaccgggtg atactgttcg gcacgtttga tgtagtagtc
    20761 actgagcgtc tgtgcggctt tcgatgtgcc gccaccgata cccattttca ggacatcacc
    20821 agccccgtaa gcggctgtgg cacccagccc gacagccggc tgggaggcgc gctccattcc
    20881 ctgaccgata ccgtcaacaa atcccgcgcc ccatgcccag ccgaggattt tgccgttacg
    20941 catcaccact tcgcccttga taccgttttt accccggaag ctgacatgcc ccttaatcgg
    21001 catatcaata gttttgccgt ccttcaggca actgatattg cgggtgcgta caatggcacg
    21061 ctcactggac acatcccccc aggcttccag ccccacaaaa catcccgttg cgtcatacgt
    21121 cttgctgttc ggcatttcca ccaggccggt gatacgcaac tgcatcggca ccgtggattc
    21181 attaccggtg actgaggcat tggcatccgc cccttcaatc agcatggctt tcgcaaaact
    21241 tcctgacgga atgtacggca gcgatggtcc ctgttttccc tcattacggg taaacacctt
    21301 acgctgtatc cggttaggca ccggcacaga ctggtacgtc acctgtggtg gtggcgtgac
    21361 accattcccg ggataaaacg ccgtcggcgg tggaacagca acgctgccct gcggcgggaa
    21421 ggatacgggg gtgtttcctg gctgtggttc gccttccggc ccgggcggtg aggcaggcat
    21481 ctgaggtaca ggctcaccgg tcgccgtgac gggattagca cccagggctt ttacctgctc
    21541 tgccagggcg gcattgtcct gtcccagctt ttcaatacga cgctggtcat caccgcgctg
    21601 tttgttcagg acatccagtt cacgacggat ttcctcatac tgcttctgca tctgcgctgc
    21661 cgtcacctgc atctctgtgg tggcatgctg acgcaccttg tcatcaaagg tcgtatccac
    21721 cacacctgtc atatccggca caggctcctg ttcagccacc gcctcaccgt taccggacat
    21781 gtccacatca gacagataca gtaccccacc aatcgcggag gctgcaccga caaccacaat
    21841 ccccagccac aggtactgct tgcgtttcac aatggtattg atactggcca tcagttgccc
    21901 tccccgtcac ggatgacata cacatccatg cgcgcacctg ccagtaactg gctggcaggc
    21961 tggctgaaca tcacggcacg ggttcccggc tgccagaagt cactttcccg gatattcagg
    22021 gcagacagcg ttttattttc cacggcaaag cggaccatct tcaggtgatt ccccgtccat
    22081 acggcatctg ccactgaaga cagccccgcc ggggcctgca gggtttcctt tgtcacagga
    22141 acctgatacc agcctgcagg taattttccg ccacggacag cctggctgat ggtcacaagc
    22201 agggattcgt aaggcgtgga cgtttcccac gctccggctt cttctccggt tccgcgcagg
    22261 tcactgacca gctgaatggt acgccccgcg ccttcacggg gaacagcctg aatggaaagg
    22321 ttcagaccac gctctgtttc cagaatgaac gtaaagggct ttttgttgac ggtggccacc
    22381 accacaccgc cggaggcggt ctgctcatta ttggtcaggg caccatccag actgttcacg
    22441 gcgataatcc ggtcaccggg aacggtaaac agattcgggc tggtattgct gatggcaata
    22501 ctggcctgac caccgttcac cattggcacc acagtggggg ccagcgtacc gtttgcggcc
    22561 atcacgctgc aggaaaaaaa caggccgccg aatattattg ccgtattatt ttttctcatc
    22621 gtctgtttcc ccgaaattat ccagccaggt caccccgttt tcccgcttca gaataaggat
    22681 gtaatgtttg atatcagtaa aaggttcgga gttaccaatc catgttttcc gaattccacg
    22741 gatttccaca cgaccatact gaggccagac acggacactg gtctggaaaa aagcagaatt
    22801 cacgttatct tttttaatga gtttcgcttc ttcagccaga ataactttca tctggttctg
    22861 tgcacccggg cggatatatt gcagaagcgc ctgatgtgaa gcatcgacga tttccggtga
    22921 gacattcaga cggagggcaa taaatgacag cgccatctgc tgcaaataag aggcatcggc
    22981 actgttctgt gacacggcaa agggcacatt aaatcccatt ggggtcacgg cagtgcgttg
    23041 ctcattctga agacggtagt tattcacccc ctgaatgagg ttaacagaga ggctgagaac
    23101 aataagcact gacataaata taaaggcgat ggccattaca cgactggtac ttaaacgggc
    23161 accgtgttcc atataacaat cctggtatca gttctattta atccactgcc ggaaacacga
    23221 atcgggaaca ttatgaaaaa taccgcgcag cagggctgtt ggcatatacc agtaaatcag
    23281 gtcacgtaac caggaactgc cccgcccttt tttcagtttt ttaatcccga aataaaccag
    23341 aaccgctgca ccaataccga acagatattt cgatgttgtg ataccccagc caacacagat
    23401 tgctgcgggg atcagttcat ccagtggcag gccaaaccag cggctctggt tggtcagtgt
    23461 ttccgggaaa cgatatttct taagtttatt ctcgtctccc gacatcgttt tatttcctgt
    23521 cagaggccaa cgacgctcat acccacagca ataaatacag agatgatggc aaagccgacc
    23581 aggaacttga cgtttttggt catcatgtac atgaccgcac cgaccagcac ctcagccaga
    23641 acaacccatt taacgatact ggagtcctta ccgaaggtcg ccttaaccgt atcgttacct
    23701 ttagccatca ggtcgccttt accagcagcc atagctaact cagggaacaa catcatcaga
    23761 acagcaaccg ggatcactgc acgaaccagg cgaagcatat tcagacgagt gaatttagaa
    23821 aaaaacgatt tattttcaac gggcgcagaa gcaccctgaa cacttaaaac agtattcata
    23881 tgattcctct gattaatatt gacagatgaa aactaaagtt ctttaaatgt tgactcgttc
    23941 tcttcgatca cctccctgaa tatttcttca ttataaaaat ctggataacg ttgcaaatga
    24001 tcctgcaacc tcaccaatgc ttcgtttgtt ttcgttcttc cactacgctc tctggctcca
    24061 agtaataatt tattcgtttc ctcatcaagg gtaagtaaaa cttttctact tggcgcagat
    24121 cttaaagctc ctttaatcac cctccctccc ataaataaaa gagatttagt caaaatattc
    24181 acataagacg cacacaaaaa taaacaacaa aatagtaaat tacgttaaca aaaaccggca
    24241 cttttagtgc cgggaatgct tacatttctt tacagtgaag gctgttaata ttactagcga
    24301 agtattgata tagataaaat aacacatcag agattatagc atatcgcaac cactcatccc
    24361 tgtctgagaa accatagtgt tttttaaaat tcataaatat ttttttagat tggtcaacag
    24421 agatatccat tctctgagaa gaatactcat gactataacc caaaagatac ggtattatct
    24481 tattaaagcg atatttgtca ggttcacaac taaactcctt gccctttata aaaaagccac
    24541 taactacgta tctttcaaag aatctccaga caaacaaatt caagttattc acttccgtac
    24601 tttcaaccac cacatcccat gatacatcat tcaatattac accattaata atactcactc
    24661 cattcgagga cgagaaagca tcaacttcag cagtatgaaa taaaatttgc gtatcaatat
    24721 caataccaga aaaccatatt tcagaatcac tattctgaca ctttattaag tgtaaaaaaa
    24781 cagagtttga gtctatgaat tttcctgact ctgtgcggac acagactgga tattttgcgg
    24841 ataaaataat ttccggcagg cctgattgac gtatgagtgc tctttctcta gtatccatcg
    24901 cacacatagg aacctcctca aaggattcta tggacagtcg atgcagggag ggacaagctc
    24961 cctgcatcgg cgattttcaa gttcaaattt taacgtggca ttaaccacgt aattaatata
    25021 ctaattattc tgattattgt catcagtttg aaccattaaa aagtctgata tttgaacaaa
    25081 gtcaaaattt atttcccttt attcatcatc attttttgga aagaaccttt ccatctcagc
    25141 cgacaccttc tctctgatat cttcaaccat attggcatat tcaaactttg gatttcctga
    25201 gacatgagga ctgagagact caatacccaa aattttcgct actgatgact gtgtttttac
    25261 aacgcattca agaagtagtt tattaaactc ggcctgatta aatgcagact ctttgcgctc
    25321 catctgagcc tcatatacac gaagacccag ttcaagaagc attgaagccg ttgctgaaaa
    25381 actgacatct ttttctctcg ccccctcctg tcgacgtttt tcaataatcg cgtttatttt
    25441 ttcataagca tcattgctga tatacagatt caccttagcc attagatatt acctttttta
    25501 aacataaaac aaagttaatt tcgaaacact agaattcata gtgcaaacag ggacgcaccg
    25561 ctagcagcgc ccctgacggt atcctataaa aaaacacacc gcgccgctag cagcacccct
    25621 aatataaaat aatgtttttt ataaaaatag tcagtaccac ccc
//
LOCUS       DAZXUK010000029        25661 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZXUK010000029 DAZXUK010000000
VERSION     DAZXUK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25661
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.029"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACH3L8_001009"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACH3L8_001009"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFT4084227.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="ACH3L8_001010"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="ACH3L8_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFT4084228.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="ACH3L8_001011"
     CDS             2630..3391
                     /locus_tag="ACH3L8_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFT4084229.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="ACH3L8_001012"
     CDS             complement(3442..4326)
                     /locus_tag="ACH3L8_001012"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFT4084230.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACH3L8_001013"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACH3L8_001013"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFT4084231.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="ACH3L8_001014"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="ACH3L8_001014"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFT4084232.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="ACH3L8_001015"
     CDS             6202..6474
                     /locus_tag="ACH3L8_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFT4084233.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="ACH3L8_001016"
     CDS             6844..7920
                     /locus_tag="ACH3L8_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFT4084234.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="ACH3L8_001017"
     CDS             7947..9326
                     /locus_tag="ACH3L8_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4084235.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="ACH3L8_001018"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="ACH3L8_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="ACH3L8_001019"
     CDS             10409..11245
                     /locus_tag="ACH3L8_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFT4084236.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="ACH3L8_001020"
     CDS             11250..12197
                     /locus_tag="ACH3L8_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFT4084237.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="ACH3L8_001021"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="ACH3L8_001021"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFT4084238.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="ACH3L8_001022"
     CDS             13324..14082
                     /locus_tag="ACH3L8_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFT4084239.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="ACH3L8_001023"
     CDS             14200..15558
                     /locus_tag="ACH3L8_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4084240.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACH3L8_001024"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACH3L8_001024"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFT4084241.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACH3L8_001025"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACH3L8_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFT4084242.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACH3L8_001026"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACH3L8_001026"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFT4084243.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="ACH3L8_001027"
     CDS             19503..20054
                     /locus_tag="ACH3L8_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFT4084244.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="ACH3L8_001028"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="ACH3L8_001028"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFT4084245.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="ACH3L8_001029"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="ACH3L8_001029"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFT4084246.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="ACH3L8_001030"
     CDS             23307..24347
                     /locus_tag="ACH3L8_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFT4084247.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..>25661)
                     /gene="gdhA"
                     /locus_tag="ACH3L8_001031"
     CDS             complement(24464..>25661)
                     /gene="gdhA"
                     /locus_tag="ACH3L8_001031"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFT4084248.1"
                     /translation="LLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKG
                     GMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLM
                     TELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPE
                     ATGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGT
                     VVDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQN
                     ELDVDAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLE
                     MAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAM
                     LAQGVI"
BASE COUNT         6518 a         5971 c         6624 g         6548 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctagctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatttgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagtaa t
//
LOCUS       DAZXUK010000030        25639 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZXUK010000030 DAZXUK010000000
VERSION     DAZXUK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25639
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.030"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(32..166)
                     /locus_tag="ACH3L8_001032"
     CDS             complement(32..166)
                     /locus_tag="ACH3L8_001032"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084249.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            242..1132
                     /gene="metF"
                     /locus_tag="ACH3L8_001033"
     CDS             242..1132
                     /gene="metF"
                     /locus_tag="ACH3L8_001033"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFT4084250.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            1461..3641
                     /gene="katG"
                     /locus_tag="ACH3L8_001034"
     CDS             1461..3641
                     /gene="katG"
                     /locus_tag="ACH3L8_001034"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFT4084251.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            3735..4640
                     /gene="yijE"
                     /locus_tag="ACH3L8_001035"
     CDS             3735..4640
                     /gene="yijE"
                     /locus_tag="ACH3L8_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFT4084252.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(4667..5274)
                     /locus_tag="ACH3L8_001036"
                     /pseudo
     CDS             complement(4667..5274)
                     /locus_tag="ACH3L8_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(5549..6650)
                     /gene="gldA"
                     /locus_tag="ACH3L8_001037"
                     /pseudo
     CDS             complement(5549..6650)
                     /gene="gldA"
                     /locus_tag="ACH3L8_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            complement(6661..7323)
                     /gene="fsa"
                     /locus_tag="ACH3L8_001038"
                     /pseudo
     CDS             complement(6661..7323)
                     /gene="fsa"
                     /locus_tag="ACH3L8_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(7335..9836)
                     /gene="ptsP"
                     /locus_tag="ACH3L8_001039"
                     /pseudo
     CDS             complement(7335..9836)
                     /gene="ptsP"
                     /locus_tag="ACH3L8_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            10145..11224
                     /locus_tag="ACH3L8_001040"
     CDS             10145..11224
                     /locus_tag="ACH3L8_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFT4084253.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            11239..11559
                     /locus_tag="ACH3L8_001041"
     CDS             11239..11559
                     /locus_tag="ACH3L8_001041"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFT4084254.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            11610..13907
                     /locus_tag="ACH3L8_001042"
     CDS             11610..13907
                     /locus_tag="ACH3L8_001042"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFT4084255.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            13873..14751
                     /locus_tag="ACH3L8_001043"
     CDS             13873..14751
                     /locus_tag="ACH3L8_001043"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFT4084256.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            14753..15094
                     /locus_tag="ACH3L8_001044"
     CDS             14753..15094
                     /locus_tag="ACH3L8_001044"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFT4084257.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(15081..15932)
                     /locus_tag="ACH3L8_001045"
     CDS             complement(15081..15932)
                     /locus_tag="ACH3L8_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFT4084258.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(16081..17814)
                     /locus_tag="ACH3L8_001046"
     CDS             complement(16081..17814)
                     /locus_tag="ACH3L8_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFT4084259.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(17997..20648)
                     /gene="ppc"
                     /locus_tag="ACH3L8_001047"
     CDS             complement(17997..20648)
                     /gene="ppc"
                     /locus_tag="ACH3L8_001047"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFT4084260.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(21000..22151)
                     /gene="argE"
                     /locus_tag="ACH3L8_001048"
     CDS             complement(21000..22151)
                     /gene="argE"
                     /locus_tag="ACH3L8_001048"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFT4084261.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            22305..23309
                     /gene="argC"
                     /locus_tag="ACH3L8_001049"
     CDS             22305..23309
                     /gene="argC"
                     /locus_tag="ACH3L8_001049"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFT4084262.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            23317..24093
                     /gene="argB"
                     /locus_tag="ACH3L8_001050"
     CDS             23317..24093
                     /gene="argB"
                     /locus_tag="ACH3L8_001050"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFT4084263.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            24154..25527
                     /gene="argH"
                     /locus_tag="ACH3L8_001051"
     CDS             24154..25527
                     /gene="argH"
                     /locus_tag="ACH3L8_001051"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFT4084264.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         5826 a         6471 c         7134 g         6208 t
ORIGIN      
        1 tccggcaaga agcaataagt acatggttag tttatatttg cagtccggtt tgctttgcat
       61 atcggatttt ctttttctta ccatcctgaa gttttttcat cttccctgat ttttcctcac
      121 catcattggt catttttcgg ttgacgccct tcggcttttc cttcatcttt acatctggac
      181 gtctaaatgg atagatgtgc acaacacaac atataactac aagcgattga tgaggtaagg
      241 tatgagcttt tttcacgcca gccagcggga tgccctgaat cagagcctgg cagaagtcca
      301 ggggcagatt aacgtttcgt tcgagttttt cccgccgcgt accagtgaaa tggagcagac
      361 cctgtggaac tccatcgatc gccttagcag cctgaaaccg aagtttgtat cggtgaccta
      421 tggcgcgaac tccggcgagc gcgaccgtac gcacagcatt attaaaggca ttaaagatcg
      481 cactggtctg gaagcggcac cgcatcttac ttgcattgat gcgacgcccg acgagctgcg
      541 caccattgca cgcgactact ggaataacgg tattcgtcat atcgtggcac tgcgtggcga
      601 tctgccgccg ggaagtggta agccagaaat gtatgcttct gacctggtga cgctgttaaa
      661 agaagtggca gatttcgata tatccgtggc ggcgtatccg gaagttcacc cggaagcaaa
      721 aagcgctcag gcggatttgc ttaatctgaa acgcaaagtg gatgccggag ccaaccgcgc
      781 gattactcag ttcttcttcg atgtcgaaag ctacctgcgt tttcgtgacc gctgtgtatc
      841 ggcgggcatt gatgtggaaa tcatcccggg cattttgccg gtatctaact ttaaacaggc
      901 gaagaaattt gccgacatga ccaacgtgcg tattccggcg tggatggcgc aaatgtttga
      961 cgggctggat gacgatgccg aaacccgcaa actggtgggc gcgaatattg ccatggatat
     1021 ggtgaagatt ttaagccgtg aaggagtgaa agatttccac ttctatacgc ttaaccgcgc
     1081 tgaaatgagt tacgcgattt gccatacgct gggtgttcga cctggtttat aaatagtgtg
     1141 gcttttgtga aaatcacaca gtgatcacaa attttaaaca gagcacaaaa tgctgcctcg
     1201 aaatgagggc gggaaaataa ggttgtcagc cttgttttct ccctcattac ttgaaggata
     1261 tgaagctaaa accctttttt ataaagcatt tgtccgaatt cggacataat caaaaaagct
     1321 taattacgat caatttgatc tacatctctt taaccaacaa tatgtaagat ctcaactatc
     1381 gcatccgtgg attaattcaa ttataacttc tctctaacgc tgtgtatcgt aacggtaaca
     1441 ctgtagaggg gagcacattg atgagcacgt cagacgatat ccataacacc acagccactg
     1501 gcaaatgccc gttccatcag ggcggtcacg accagagcgc gggggcgggc acaaccactc
     1561 gcgactggtg gccaaatcaa ctccgtgttg acctgttaaa ccaacattct aatcgttcta
     1621 acccgctggg tgaggacttt gactaccgca aagaattcag caaattagat tactacggcc
     1681 tgaaaaaaga tctgaaagcc ctgctgacag aatctcagcc gtggtggcca gccgactggg
     1741 gcagctacgc cggtctgttt attcgtatgg cttggcacgg cgcaggtact taccgttcaa
     1801 tcgatggacg cggcggcgcg ggtcgtggtc agcaacgttt tgcaccgctg aactcctggc
     1861 cggataacgt aagcctcgat aaagcgcgtc gcctgttgtg gccaatcaaa cagaaatatg
     1921 gtcagaaaat ctcctgggcc gacctgttta tcctcgcagg taacgtggcg ctggaaaact
     1981 ccggcttccg taccttcggt tttggtgccg gtcgtgaaga cgtctgggaa ccggatctgg
     2041 atgttaactg gggtgatgaa aaagcctggc tgactcaccg tcatccggaa gcgctggcga
     2101 aagcaccgct gggtgcaacc gagatgggcc tgatttacgt taacccggaa ggcccggatc
     2161 acagcggcga accgctttct gcggcagcag ctatccgcgc gaccttcggc aacatgggca
     2221 tgaacgacga agaaaccgtg gcgctgattg cgggtggtca tacgctgggt aaaacccacg
     2281 gtgccggtcc gacatcaaac gtaggtcctg atccagaagc tgcaccgatt gaagaacaag
     2341 gtttaggctg ggcgagcact tacggcagcg gcgttggcgc agatgccatt acctctggtc
     2401 tggaagtagt ctggacccag acgccgatcc agtggagcaa ctatttcttc gagaatctgt
     2461 tcaagtatga gtgggtacag acccgcagtc cggctggagc aatccagttc gaagcggtag
     2521 acgcaccgga aattatcccg gatccgttcg atccgtcgaa gaaacgtaaa ccgacaatgc
     2581 tggtgaccga cctgacgctg cgttttgatc ctgagttcga gaagatctct cgtcgtttcc
     2641 tcaacgatcc gcaggcgttc aacgaagcct ttgcccgtgc ctggttcaaa ctgacgcaca
     2701 gggatatggg gccgaaatct cgctacatcg ggccggaagt gccgaaagaa gatctgatct
     2761 ggcaagatcc gctgccgcag ccgatctaca acccgaccga gcaggacatt atcgatctga
     2821 aattcgcgat tgcggattct ggtctgtctg ttagtgagct ggtatcggtg gcctgggcat
     2881 ctgcttctac cttccgtggt ggcgacaaac gcggtggtgc caacggtgcg cgtctggcat
     2941 taatgccgca gcgcgactgg gatgtgaacg ccgcagccgt tcgtgctctg cctgttctgg
     3001 agaaaatcca gaaagagtct ggtaaagcct cgctggcgga tatcatcgtg ctggctggtg
     3061 tggttggtgt tgagaaagcc gcaagcgccg caggtttgag cattcatgta ccgtttgcgc
     3121 cgggtcgcgt tgatgcgcgt caggatcaga ctgacattga gatgtttgag ctgctggagc
     3181 caattgctga cggtttccgt aactatcgcg ctcgtctgga cgtttccacc accgagtcac
     3241 tgctgatcga caaagcacag caactgacgc tgaccgcgcc ggaaatgact gcgctggtgg
     3301 gcgggatgcg tgtactgggt gccaacttcg atggcagcaa aaacggcgtc ttcactgacc
     3361 gcgttggcgt attgagcaat gacttcttcg tgaacttgct ggatatgcgt tacgagtgga
     3421 aagcgactga cgaatcgaaa gagctgttcg aaggacgtga ccgcgaaacc ggcgaagtga
     3481 aatacacagc cagccgtgcg gatctggtat ttggttctaa ctctgtcctg cgtgcagtag
     3541 cggaagttta cgccagcagc gatgcccacg agaagtttgt taaagacttc gtagcggcat
     3601 gggtgaaagt gatgaacctc gaccgtttcg acctgctgta atctgacctt cttcagcgac
     3661 tgtctttcag gcagtcgctg aagtttcttt accggcgtat agtgtccaca ggaaaactac
     3721 acactggatc tctcatgtct gccgcaggaa agagcaaccc actggcaatc agtggcttgg
     3781 ttgtgctcac acttatctgg agttatagct ggattttcat gaagcaagtc accagttaca
     3841 tcggtgcctt cgactttacc gccttacgct gcattttcgg cgctctcgtt ttattcatcg
     3901 tccttttatt acgtggtcgc ggaatgcgcc cgacgccgtt taaatacacc ttagccattg
     3961 ccctgttaca aacctgcggg atggttggtc tggcgcagtg ggcgttggtc agcggaggtg
     4021 cggggaaggt ggcgatcctg agctatacca tgccgttctg ggtggtgatt ttcgccgcgt
     4081 tgtttctcgg tgaacgcctg cgacgtgggc aatatttcgc gattctgatt gccgctttcg
     4141 gcttattttt ggtgttgcag ccgtggcaac tcgatttctc ttcgatgaaa agtgccatgc
     4201 tggcaatcct ctccggtgtt agttgggggg cgagcgcgat tgttgctaaa cgcctatacg
     4261 cccgtcatcc gcgcgtggat ttattatcgt taacatcctg gcagatgctg tatgcggcgc
     4321 tggtgatgag tgtggtcgct ttactggtgc cgcaacgtga aattgactgg cagcccaccg
     4381 tgttctgggc gctggcctac agtgcgattc tggcgacggc gctagcgtgg agcttatggc
     4441 tgtttgtgct gaaaaacttg cctgccagta ttgccagctt aagcacactg gccgttcccg
     4501 tttgtggcgt actcttttcc tggtggctgc tcggtgagaa tccgggggcc gttgaaggta
     4561 gcggtattgt gctgattgtg ctggcactgg cgctggtgag ccgtaagaaa aaagaagctg
     4621 tcagtgtaaa aagcatctga attttttctt catgtggggc gatctcttat ttaacaaaat
     4681 aacgataatg ccccaccatc cgccagctaa acagcacatc ttcttcctgc gcgcctgcgc
     4741 caatgttatg tatcaccagc ggcgtaccgt cgcgggcgaa gccatctgac gccaccccaa
     4801 tatgcgccag cccgttatct aatcgccagg agacaatatc gcccgcttga tagtcaccgg
     4861 catttttact gatggggcgt gttttgttgt ggcggctaaa ccaggtttcc agattaggca
     4921 cccgacggtg atcgatgttg ctgtccgggc gctttaactt ccatttttgc gggtactcag
     4981 caaaattctt cgccatatct tcgtgaacca gtttctgcaa atcgaccttc tggctgcgca
     5041 atgcgcggat caccacatcg gaacatacac cgcgttcttg cggaacatca ccgccaggat
     5101 aggtaagctg cgatcgtaaa atagcgtgct gccaatttgc tgtctggcac cgtctgcgat
     5161 ggcaaggttg gtattggcct ggatttgtac cacggttggt ggaacggcgg gagattttaa
     5221 ggagtggctg gtaaatgccg ttagcaggct gagcagcgcc agtgaagctt tcatctggtt
     5281 cgtccgtgaa aataagagtc acagattcga ccttccgggc taacgattgt tctgtggaga
     5341 aaatgtccag aagcggataa aaaactcctg aacaacgtgg gagtttcaga ttactggcac
     5401 agagtgccgg atgcggagtg aacgccttat ccggcctaca aaagcacgca aattcaatat
     5461 attgcagaga ttgcgtaggc ctgataagcg tagcgcatca ggcaattttg cgttcaaact
     5521 cccggacaag ccgggagttt ggagtaggtt attcccactc ttgcaggaaa cgctgaccgt
     5581 actggtcagc taccagcagt gcggcgtaaa cctgatctgg cgtcgcgccg ccaggcatgt
     5641 tgtggatggt ttcaccttct gcacaagatg cttttgcaac aatgcgcatt ttcgccggga
     5701 catcttcttt aatatccagt tgagcgagag ttattggcaa acctaccgca tggctaagtg
     5761 cagctacggt ttcgatttcc tccaccggcg cgttttccag taccagctgc gtcagcgtac
     5821 caaatgccac ttttcaccgt gataatagtg atgcgcgtcc gggatagcgg tcaggccgtt
     5881 atgcaccgcg tgagccgccg ccagccgccg ctttcaaaac caacgccgct caaataggtg
     5941 ttcgcttcaa tcacgcgctc cagcgccggg gttactacat gctgttcggc ggcaagcatc
     6001 gctttttcgc cttcttccag cagggtgttg tagcacagtt cagccagtgc cagcgccgcc
     6061 tgggtgcact tgccgcccgc catggtggtc gcgccgctac gagagcaagc acgcgcttca
     6121 aaccaggttg ccagcgcatc gccgataccc gctgctaaca gacgtgcagg tgcgcccgcg
     6181 acgattttgg tgtcgacaat gaccatattc gggttatttg gcaacagcag atagcggtca
     6241 aactcaccct catcggtgta gataacagac aatgcgctgc acggtgcatc ggtcgaggcg
     6301 atagtcggtg cgatcgctac cggaacaccc atgaaatgtg ccagtgcttt ggcagtatcg
     6361 agggtttttc cgccaccgat accgagaatt gcgccacact gcgcagtctc cgcgatgcca
     6421 cgcagacggt cgatctcatt ttgcgaacat tcaccgccaa acggcgtaat ttctactacc
     6481 agtccagcat ctttaaagct tttctcgaca gtggattgag caaaaactaa aacaaatttg
     6541 tcacccacca ctaaccagcg ttctgccagc ggcttcaggt attcgcccag acgattaatc
     6601 acatcagcgc cctggatgta tttacccggt gattgaataa tgcggtccat aattgctcct
     6661 ttagagatga gtagtgccaa atgcggcatt ccagtcgtgt tcgaacttct ctatagctga
     6721 ctctaccgca ggggtgttga gcatttgttg cgctacatct aagggcaggg tgatggattc
     6781 acatcctgcc agtaaacagt ccagcgcctg acgcggcgtt ttaaagctgg ctgccagcac
     6841 catgctttct ggcgcgtgca tttctaacag cgtttgcagc tcctgaaccg tacgaatgcc
     6901 gtccccgccc tgggcatcta cgcggttaac atacggagca acgtattttg cccctgccag
     6961 tgcggctaat aacccttctg cggcgctata tacagcggtg ccaagtgtag taatacccta
     7021 ttttttcagt attttaattg ctgccagacc ttcggaagtc accgggattt tcaccacaat
     7081 acccggaata gcgtcgcgca ggcgcttcgc ttcttccacc atcccctgcg cgtcgcggct
     7141 catggtctga gcaaacagaa tgccctcatc accaatcgct ttttgcagac gcggcagcac
     7201 ttcccagatg gactccttgc tggcagcgat aatgctcggg ttagttgtca ccccggcaat
     7261 cgggaatatg cgtgccagac gttcgacttc tgcgacgtta gcggtgtcca gatacagttc
     7321 catgatgttt ccttttacag ttccagttca tgttgcagca ggctggcgat agcgtcggca
     7381 gaggcggcgt taaccagcgc gttacggaat tcttcgtgca tgatgcggcg agcgaggcgc
     7441 gagaaaatgc gcatgtgctg atcgcccgca gcgtgtttgt tcagggttaa cataatgatg
     7501 aattgcgctt catcatcgcc ccagcgcacc ggcgcttgca gacgcgccac gctgatggtg
     7561 gattgctcaa tgtgttctga tttgctgtgt ggaatggcaa aactaaagcc cagaccggta
     7621 gagaaaacgg cctcgcgcgc ccataagtcg gcttccagtt tacgcggata gcggcagcgg
     7681 cccgccagca gcaggttatc ggtcatgcct ttgagcactt cttctttgct gcgccagtca
     7741 ctttccagcg tgatgcactc ggcggtgacc agcggtgcgt cctgttgggt catgcggaat
     7801 tgcgccagca ggtgttccac ttccagcgaa gtacggcagg ccattgcctg gttgagcaac
     7861 ttgcggcact cacggctatc aagttgcgcc atccgcgctt tcgccgccgg aattgatggt
     7921 gcgctcatgc tgagttcatc cagccctaag ccgaccagca acggcagcac ggaacctttc
     7981 gctcccagct caccgcacag accaatccat ttaccctggc gatgcaccgc ctgcacgtcg
     8041 taatcgagcg cccgcaagaa tgccggattc aggctgttgt agtgacgagt aaccttagcg
     8101 ttatcgcgat ccactgccag cagatattgc gtcaggtcat tactaccaat actaaagaaa
     8161 tcaatctctt cgcagcattg atcgatgatg aacatcaccg acggcacttc cagcataatg
     8221 ccgagctgga tcttctcatc aaacggaatg tgttcgttac gtagttgctg tttggcttct
     8281 gccagttttt ctttcaccca taagatctct tccattgagg agatcatcgg gatcatgatt
     8341 ttcaggctgc cgtgagcgga ggcgcgcagg atcgagcgta gttgtgtggt aaataacgac
     8401 gcgtattctt cataaatacg cacggcgcga taaccgagga acgggtttgc ctctgcggga
     8461 atgtttagat aatcaacggg tttgtcgccg ccaatgtcca tagtgcgcac aataatgctg
     8521 cgtccgttgg cagactccag cgcctgacaa aaaatgttgt acagctcgct ttcgcccggt
     8581 gcactggtgc gatccatata gagcatttca gtgcggaaca aaccaacgcc ttccgcacca
     8641 ttgccgaatg cggcctgcgc ttccacggag tgagcgatgt tagcggcaat ttcaatgcgg
     8701 ataccgtcag cggtacgggc ttgttgggtc agctagacac gctgttgctc acgcagggcg
     8761 tcctgtacgc gggcttcttg ctgataataa cgagctacgg cttctcctgg ctcaaccaca
     8821 atcgccccgg cgttgccgtc gatataaatc gtttgatgct gccacggagt gagggcatca
     8881 atatccacac caaccagcgt cggaatgttg aacgaacggg caaggatcac cgtatgtgag
     8941 gtggtgccgc cgcttttcag taacaatcct ttgaggtgat ttttatccag ttcgaggaac
     9001 tggctggggg tcagttcatc agccatacaa atggcggact gcgtcagttt gcccggtgcc
     9061 gggaagcgtt gctcaccgta gatttgctgg agtaactgga agcagacgtc gcgtacgtcc
     9121 agggcacgtt cttgcaggta tctgctgctg gaacgggcaa actcttcgca aaagtgattc
     9181 gcactggcga caatcgcttc ggcgcagctt aatccggcgc tgacgcctgc cagtaaatgt
     9241 tcgtgcaggg aagtatcgcc agccagggat cggtgcgctt ccagaatcgc gctggtagca
     9301 ccgtcgctat ccagcagacg aaactcaatg tttttcagta ccagcgtcag gccgttttcc
     9361 agtgcggatt gctcggcgtc aacgtctttg gctgcgggaa gattactcag cgcattgaga
     9421 tctaaagaag agatcggcgt cagaatgccg cctgcactac cgctgcacac ggtgcgggcg
     9481 cggataattt gcggattcag attggtcagt gaaaccggca gtggttccag ttcgtcagat
     9541 ttaacttccg ccagcggcgc atcgcagtgg gggaattcat cgcgcagcca ttggcttaaa
     9601 cgctggtgcc cttcctgttc gtcggccccg gaaatcagta gctggcagtt atcacccacc
     9661 agcgtatcgg cgccaatcag cgccagagcg cttttggcgt tgcctttacg gtcagtacgc
     9721 aggttatgcc actcaatttg agatgaaaaa gtattacaca gcgtttcaac gtggcttgcc
     9781 ggacgcgcat gtacgccgtt aggtagctca caaataaatt ccacaatcag ggccattgcc
     9841 tttctcccgg tacggttttc tgtttacagg ataagagcgc acggtaacgg cctgccatgt
     9901 gacaaatctg ccaaaagctg gacaaatgta atgtaaccgt caatttgcga cgcgtctcac
     9961 aagacgccgt tttgcggcat gcttccggat tatcgcaagt tatgaggcgg atcgcatttt
    10021 tgtactgata ttacaaaagt ccagtaaatg gcctttttat ccactgtttg ctccgcctgc
    10081 gattgcctat tgtttcctcc atcaaaaata tggacatggg cttttgcaag cccgggagca
    10141 gcttatgaat gagttggtgc agatcctgaa aaatacccgt cagcatttaa tgacgggcgt
    10201 ttcacacatg attcccttcg tggtatcggg cggtattttg ctggcggttt ccgtcatgtt
    10261 gtatggcaaa ggcgcagtgc cggatgccgt agccgatccg aatttgaaaa aactgtttga
    10321 tatcggcgtt gcaggtttga cgctgatggt gcctttcctc gccgcttaca ttggttactc
    10381 cattgcagag cgttctgcgc tggctccgtg cgctatcggt gcctgggttg gtaacagctt
    10441 tggtgcgggc ttctttggtg cgctgatcgc cgggattatc ggcggcatcg tggtgcatta
    10501 cctgaagaaa attccggtgc ataaagttct gcgctcggtg atgcccatct tcattattcc
    10561 tatcatcggc acactgatta ccgcaggcat catgatgtgg ggcttgggcg agcctgtagg
    10621 ggcgttgacc aacagcctga ctcagtggct tcaggggatg cagcagggca gcattgttat
    10681 gctggcggtg atcatggggc tgatgctggc gttcgatatg ggcggtccgg ttaacaaagt
    10741 ggcctatgcc ttcatgctga tttgcgttgc tcagggtgtt tataccgtgg tggccattgc
    10801 tgccgtgggt atttgtatcc cgccgctggg gatggggctg gcgacgctga ttggtcgtaa
    10861 aaatttctcc gcagaagagc gcgaaaccgg caaagcggca ctggtgatgg gctgtgttgg
    10921 cgttactgaa ggggcgattc ctttcgccgc tgccgatccg ctgcgtgtca ttccttccat
    10981 tatggtcggt tcagtttgtg gtgcagtaac tgcggcgctg gtcggtgcgc agtgctatgc
    11041 aggctggggt ggtctgattg tgctgccggt ggttgaaggt aagttgggtt atatcgcagc
    11101 agtggctgtc ggagcagtgg tgacggctgt ttgtgtgaac gtgctgaaaa gtctggcgcg
    11161 taaaaatggg tcttcgactg atgaaaaaga agacgacctg gatttggatt ttgaaattaa
    11221 ttaattgagg aacgagccat gacgaaaatt attgcagtaa ccgcatgtcc ttcaggtgtt
    11281 gcccatacct atatggcagc agaggcgctg gaaagtgccg cgaaagcgaa aggctgggaa
    11341 gtgaaagtag aaacccaggg gtcaattggt ctggaaaatg aactgactgc ggaagacgtg
    11401 gcgagcgctg atatggtgat tctgaccaaa gatatcggca tcaagtttga agagcgtttt
    11461 gctggcaaga ccatcgtgcg cgtcaacatc agcgatgcag taaaacgtgc tgacgccatt
    11521 atgagcaaaa ttgaagctca cctggcgcaa accgcttaag ttcttctccc gctcgcaagg
    11581 gcgggttcgc tttcccacag gagttcctca tgacgaatcg tatctctcgc ctcaaaactg
    11641 cactgtttgc caatacccgt gaaatctcgc tggagcgggc gctgctttat accgccagcc
    11701 atcggcaaac cgaaggcgaa ccggtgatat tgcgccgggc gaaagcaaca gcgtatatcc
    11761 ttgaacatgt tgaaatttcg attcgtgatg aagaactgat tgccggtaac cgcaccgtaa
    11821 aaccgcgcgc cgggattatg tcgccggaaa tggaccctta ctggctgctg aaagagctgg
    11881 atcaattccc gacgcgtccg caggaccgct ttgctatcag cgaagaagat aaacgtatct
    11941 accgtgaaga gttgttcccg tactgggaaa aacgttcgat gaaagatttc atcaacgggc
    12001 agatgacaga tgaagtaaaa gccgcgacca acacgcagat tttcagcatc aaccagacgg
    12061 ataaaggcca ggggcacatt attattgatt acccacgcct gttgaatcac ggtctgggtg
    12121 agctggtggc acagatgcag caacattgtc agcaacagcc ggagaatcac ttttatcagg
    12181 ccgcgttgtt actgctggaa gcctcgcaga aacacatttt gcgttacgcc gaactggcgg
    12241 aaacgatggc ggcaaactgc acagatgccc agcgtcgcga agagctgctg actattgcgg
    12301 agatctcccg ccataacgcg caacataagc cgcagacgtt ctggcaggcg tgccagttat
    12361 tctggtacat gaacatcatt ctgcaatacg aatccaacgc cagttcgctg tctttagggc
    12421 gcttcgacca gtatatgttg ccgttctatc aggcatcttt aacccagggc gaagatccgg
    12481 cgttcctgaa agaactgctc gaatctttat gggtgaaatg caacgacatc gtgctgttgc
    12541 gctccaccag tagcgcgcgt tatttcgccg gtttcccgac cggctatacc gcactgctcg
    12601 gcgggttaac cgagaacgga cgtagcgcgg tgaacgtgct ttcgttcctt tgccttgacg
    12661 cctatcaaag cgtgcaatta ccgcaaccga acctcggcgt gcgcactaac gccttgatcg
    12721 acacgccgtt cctgatgaaa accgccgaaa ccattcgcct cggcaccggt attccgcaaa
    12781 tctttaacga tgaagtggtg gtgccagcgt tcctcaaccg tggcgtttcg ctggaagatg
    12841 cgcgcgacta ttccgtagtg ggctgtgtgg aattatctat tcccggcaga acctacggct
    12901 tgcatgacat cgcgatgttt aatctgctga aagtgatgga aatctgcctg catgaaaatg
    12961 aaggcaatgc tgcgctgact tatgaaggtt tactggagca gatccgcgcc aagatcagcc
    13021 actacatcac cctgatggtt gagggcagca atatttgtga tatcggccat cgcgactggg
    13081 cacctgtacc gctgctctcg tcttttatca gcgattgtct ggaaaaaggt cgcgatatta
    13141 ccgatggcgg cgcgcgttat aacttctccg gcgtacaggg aattggtatc gccaacctga
    13201 gcgattctct ccatgcgttg aaagggatgg tttttgagca acagcgttta agttttgacg
    13261 aattgctgtc ggtattaaaa gccaactttg caacgccaga aggcgaaaaa gtccgcgctc
    13321 gcttaattaa ccgctttgag aaatacggta acgatatcga cgaggtggat aacattagcg
    13381 ccgaactgtt gcgccactac tgcaaagaag tggaaaaata ccagaacccg cgcggcggtt
    13441 acttcacgcc gggatcgtat accgtttctg ctcacgttcc gttgggatcg gtggttggcg
    13501 cgacgccaga cggtcgtttt gccggggaac aactggcaga cggcggcttg tcacctatgc
    13561 tgggtcagga cgcacaaggg ccaacggcgg tactgaagtc agtcagtaag ctcgataata
    13621 cgctgctgtc taacggtacg ttgctgaacg tgaaattcac tccggcgacc ctggaaggtg
    13681 aagcgggatt acgcaaactg gccgacttct tacgggtgtt tacccagctt aagttgcagc
    13741 atattcagtt taacgtggtg aacgccgaca cgttgcggga agcgcaacag cgcccacaag
    13801 attatgccgg gctggtggtg cgcgttgccg gatacagcgc cttctttgtc gaactgtcga
    13861 aggagatcca ggatgacatc atccgccgga cagcgcatca gctgtaacgt tgtggaaacg
    13921 cgccgcgatg atgtggcgcg cattttcaac attcagcgtt attcactgaa tgacggcgag
    13981 ggcattcgta cggtggtctt ttttaaaggc tgtccgcatc tttgcccgtg gtgtgctaat
    14041 ccggagtcga tctccggcaa aatccagacg gtacgcagag aggcgaaatg tctgcactgt
    14101 gcgaaatgtt tgcgtgatgc ggatgaatgc ccctccgggg cgtttgaacg gattggtcgc
    14161 gatatcagcc ttgacgctct ggaacgggaa gtgatgaaag atgacatttt ctttcgcacg
    14221 tccggcggcg gcgtcacgct ttctggcggc gaagtgttaa tgcaggcgga gtttgctacc
    14281 cgttttttac agcgactgcg gctgtggggt gtctcatgcg ccattgaaac tgccggagac
    14341 gcgccagcaa gcaagctgtt accgctggcg aaattgtgcg atgaagtgct gttcgattta
    14401 aaaattatgg acgcgactca ggcgcgggat gtggtgaaga tgaacctgcc acgcgtgctg
    14461 gagaatctgc gtttgctggt gagtgagggt gtcaacgtga tcccgcgttt accgttgatc
    14521 cctggtttca cgctcagccg ggagaatatg cagcaggcgc tggatgtact gatcccgctg
    14581 aaaatcaggc agatccatct gttaccgttt catcagtacg gcgaaccgaa ataccgcctg
    14641 ctggggaaaa catggtcgat gaaagaggtg cctgcgccgt cgtcagccga tgtgacaacg
    14701 atgcgcgaaa tggcagaacg ggccggattt caggttaccg tgggaggtta aaatggcata
    14761 cctggtggca gtaaccgcct gcgtaagcgg cgtggcgcat acttatatgg cggcggaacg
    14821 gctggaaaag ttgtgccagt tagagaagtg gggcgtcagc attgaaactc agggcgcgct
    14881 gggaacggag aatcgtttag cggacgagga tatccgtcgg gcggatgttg ctctgttgat
    14941 tacggatatc gagcttgccg gtgccgagcg atttgaacat tgccgctatg tgcaatgcag
    15001 catctacgca ttcctgcgtg agccgcagcg ggtaatgagc gcggtgcgca aagtgctttc
    15061 tgcgccgcag caaacccatc ttattctgga gtagtcggtt tttctgtcag ttggctgtgg
    15121 tactgccggc gatattccga cggcgagtgt tcggtgtttt tacgaaacag acggcagaag
    15181 tagttgctgt cgacaaagcc gcaggcgtgc gccacttctt ttaccttcag gtcatagcct
    15241 ttcaataacg tcttagcgtg ctccagtcgc gtgtgattca ggtattcgtt aaaaccaatg
    15301 gcccccgttt tttgaaacag gtgcgagaga tagtttggtg aaatatagaa tgcctgtgcg
    15361 acagattcgc gggtaagcgc ggaggcatag cgttcgtcga tataatcgcg aatagcttca
    15421 aatagtgcct ggctgcgtga ggcggtctgg atttggctgc cgagcaaatc gcggcagtgg
    15481 ctaagcaggc tggctacgat aagccttgcc gtttgctgct cctgcggctg catttgcatt
    15541 tcattgagcg tttgtagcag aaaagaacct atgcgtgggc cgcgtcgggc gacgtgttgc
    15601 ttcgccagat tttgatattg tttgccatcc cattgcacga cgctgaagcc gagttgctgt
    15661 ttgccaaaca gcacgctaaa ggtggtagcg ggtgttttcc attgtgggaa attccagcca
    15721 ccagcggcaa cgtacagcac atcgccggga actaacgttg ctccagcgcc ggtatcaaca
    15781 aactcacctt ccagcacaat ttccagccgt ggaaaatcga cctgatacgc aagatcgggc
    15841 accgggccgt tactgctggc aaagtaaatc tggcgcaggg acagcgggcc gttgatcagg
    15901 cgggagagca gatagctgac gtcgtgatac atgttattcc cttaggtatg tagacgggaa
    15961 aacagtaact ggcattatta aaaaaggcca cctgaaaagt gtggcctgag gggagttcga
    16021 tgtaaacatt gcggcagtat cgcatccggc aaatagcgcc agacgcgata caaaggtcat
    16081 ctactgatta cccacctgat cgccatacgg cagagtgtcg taatcgatca gcgcgttttt
    16141 cttgtacggg ttaccaatcc agcgggtagt ttctttgaac tgcggattca ccactgaacg
    16201 ggttggatcg taaccgtcgt aagataagcc cgccaaatct gaccaggtgt ggatcagttc
    16261 cgccaggctg tatttacgat cgacatcctg tgagaaatca cggggatgag tcgcttgcca
    16321 tttttccgac gtccacagca ggaacggaat ggtgtacatg tggcgcgtcg ggttgtcttc
    16381 gttacgcccc tgagttttgt gcggcggcgt gtcgtaaacc tcttcaccgt ggtcagagaa
    16441 gtaaaccagg aatccgttcg ggtctgccgc tttaaagtct ttaatcaggc tggcaaccac
    16501 atgatcgtta tacaggttag cgttgtcata atcgttatat gattccagct cttctgcgct
    16561 taatcctggc ggaacatgat cggtattgcc atcaaacttg ccctgatttt ccgggtagcg
    16621 gtatttgtat ttgatatgcg tacccagcag atgaacgatg atcagtttct tcggcgcagg
    16681 gtcattcagc acatcctgga acggcttcag cacgttggtg tcgtattcac gcgcactctg
    16741 cgtacgttgc tggttcatgt agtactgctt gtcggtctgg cgcgaaaata ccgtcagcat
    16801 ggtattgcgg gcggtcatcg tctgctggtt ggtgatccag aaggttttat aacccgcctg
    16861 tttcatcatg ttcatcagcg acggctgcgt cagatacaga tccgggttct tttcattggc
    16921 aaaggtcagc gcctgttgca ggatttcaat ggtgtacgga cgagacgtaa ctacgttatt
    16981 aaacacggtc agattcggat cggttttatg cagcgcatcc agctccggcg tggtttcacg
    17041 cggataaccg tacagactca tgcgtccgcg ctgggtcgac tcgccaatca ccagcactaa
    17101 agtgcgcggt tcgttacccg attcatcttt gaaattagcc agtggcggca aggcattatt
    17161 ttcattcagt aactttgtta gcgagtttag ttgctgacga tactgataat agccggtcag
    17221 gaattgccac ggtgcggcag gctccatacg cgaggctagg ttatccaacg ttttctcaaa
    17281 cggcttgttt ttgataaacg tattcatggc gatcggatgc agaatcaagc cataaagcag
    17341 ggcaaaagag acaacataac gccacggctt tggaatatag accgggcgca ggcgtgtcca
    17401 cagcagaact gccaccgccg tataggccag cgcgataagc acaattttca ggctgaaata
    17461 ctggcttaaa tactcgctgg cttcgttggt gttggtttcg aacatcacaa acagaacgct
    17521 ctgcgagaac tcctgcccgt agatgacgta gtagcacagc gccgccagag aggccgtcca
    17581 tagcaccacg ccgatcactg cggcaataat tttaatccgc ttcggaaaga ggaataccgg
    17641 gatcaaccac agcgagctga ataacagcga gtcgcgaatg ccgttggtgc cactataacc
    17701 actgatgtaa ataatggcct gtagcagagt agagaaaaac caaaagtaga gcagtgccca
    17761 acccagggct ttccagctag aaaggggttt agcctggact tctgtggaat gcatagtaag
    17821 aacctgtctt gaaaaaaata tcgccgaata taacgacaat tccttaagga tatctgaagg
    17881 tatattcaga atttgaataa aatgcagaca gaaatatatt gaaaacgagg gtgttagaac
    17941 agaagtattt cagaaaaccc tcgcgcaaaa gcacgagggt ttgcagaaga ggaagattag
    18001 ccggtattac gcatacctgc cgcaatcccg gcaatagtga ccattaacgc ttgttcgacg
    18061 cgaggatccg gttcctggcc ttctttttct gcctggcggg agcggtgcag caactcggcc
    18121 tgcaatacgt tcagcgggtc ggtgtaaata ttccgtagct gaatagactc tgcaatccac
    18181 ggcagatcgg ccatcagatg ggaatcgttg gcaatcgcca gcaccacttt gatgtcttct
    18241 tcttgcaggt tgcgtaactc tttacctaac ggccacagtg ctttgtctac cagacgttga
    18301 tcatagtatt ccgccaacca caggtctgct ttggcgaaga ccatctccag catgccgaga
    18361 cgcgtcgaga agaatggcca atcgcggcac atggcttcca gctcgctctg tttgccatct
    18421 tcgaccactt tttgcagcgc cgtacctgca cccagccagg cggggagcat caggcggttt
    18481 tgcgtccagg caaaaatcca cggtatggcg cgcagcgact cgacgccgcc ggttgggcga
    18541 cgtttcgccg gacgtgaacc caacggcagt ttgcccaatt cttgttccgg cgtagcggag
    18601 cggaagtaag gcacaaaatc tttgttttca cgtacgtagc cacggtagag atcgcaggag
    18661 atgactgaca gttcatccat aatgcgacgc cagctctctt tcggctcagg tggtggcagc
    18721 aggttggctt ccagaatcgc cccggtgtaa agcgacaggc tgctgacggt gatttctggc
    18781 agaccatatt taaagcggat catctcgccc tgttcagtca cgcgcaggcc acctttcagg
    18841 cttcctggcg gttgtgacag cagcgccgca tgggcaggtg cgccgccgcg accaatggaa
    18901 ccgccgcgac cgtggaacaa cgtcagctca atacccgctt tttcgcaggt tttgattaat
    18961 gcatcctgtg cctgatattg cgcccaggaa gctgccatca cgcccgcatc ttttgctgag
    19021 tcggaatagc caatcatcac catctgtttg ccctgaatca ggccgcgata ccagtcgata
    19081 ttgagcagct gggtcatgac atcgttggcg ttgttcaggt catcgagggt ttcaaacagc
    19141 ggagcaaccg gcatcgcaaa cccgataccc gcttctttca gcagcaggtg gacagccagt
    19201 acgtcggacg gcgttttcgc catcgagatc acgtaggcgg caatggagcc ttgcggcgct
    19261 tcggcaatca cctggcaggt atcgagcact tcgcgcgttt cggcgcttgg ttgccagttg
    19321 cgcggcagaa gcggacgttt ggagttcagt tcgcggatca ggaacgcctg tttgtcggcc
    19381 tctgaccagc tttcgtagtc gccgataccg aggtagcggg tcagctcgcc cagcgcttcg
    19441 gtatggcggg tgctctcctg acggatatca atacggacca gcggtacgcc gaaacatttc
    19501 acgcggcgca gggtgtcgag cagatcgccg ttggcgataa tacccatgcc acacgcctga
    19561 agtgactggt agcaagcgta gagcggttcc cacagttctt cgttttgtgt cagcagccct
    19621 tctggttttg gcagttcttc gcctttcagg cgcgcttcca gccatgcctg tgtcgccatc
    19681 aggcgagaac gcaggttttt catcagatag cgatacggtt ctgcggcacc ttcttcgcca
    19741 accagcgcca gcagttcagg ggtcgcttca accatcgaca gttcagaaac cagaacctga
    19801 atatctttca ggaacagatc ggtggctttc cagcggctga gcagcaggac gtggcgggtg
    19861 atatcggcag tgacgttcgg gttgccgtcg cggtcaccgc ccatccacga ggtaaaacgt
    19921 accggaacaa attcgacggg cagtttgtag ccgagattct cttccagttg ttcgttcagt
    19981 tcgcgcaggt aattcggtac gccttgccac aggctgtttt ctactacggc aaagccccat
    20041 ttggcttcat ctaccgggct tggacgcagc ttacggattt catcggtatg ccatgactgg
    20101 gcgatcaact ggcgcaggcg acgcatcagc tggttgcgtt cgtagtcagc gatatctttg
    20161 ttatcgagct gttttaaaca ggcgttcact tccaccattt tgtggatcag tgtacgacgg
    20221 gtaatttcgg ttgggtgagc cgtgaggacc agttccagcg acagcgattc cactgctttt
    20281 ttgatggtgt cttcgctcag ttccggctgg tttttcagtt tacgcagggt gcgggcgatc
    20341 acttccgggt tgctggcagc ttcgcctttc ggcgaaatgc tgtggtattg ctcggcggtg
    20401 ttggccaggt tcaggaactg actaaacgca cgcgcaacgg gcagcagctc gtcgttcgac
    20461 aaattttgta aggtggtgag caactcctgg cggttagcat cattgccagc gcgtgaagat
    20521 ttcgacaact tacggatagt ttctacgcgt tcaagaatgt gttctcccaa cgcatccttg
    20581 atggtttctc ccagcacttt gccgagcata ctgacattac tacgcaatgc ggaatattgt
    20641 tcgttcatat taccccagac accccatctt atcgtttgat agccctgtat ccttcacgtc
    20701 gcattggcgc gaatatgctc gggctttgct tttcgtcgtc ttttataaag ccacgtaaaa
    20761 gcggtgacgt caaatgctgc gaaatcgctt cagcaaacga ataaatagca ggaatttacg
    20821 ccattaaatt cacgacgctt taaataagcg taacttatgg aaatgttaaa aaatcgccat
    20881 acataacacc aaaggtgtag gtcggataag atgcgcaagc atcgcatccg acattattgc
    20941 ggcaccggag tttggcaacg gtgccggatg cggcgcgagc gccttatccg gcctacgttt
    21001 taatgccagc aaaaatggtg aattacctgg ataatcaact cacgggtggg tttaataaac
    21061 cgcgtctcca gatattcatc aggctgatga gcttgattga tggaacccgg accgagcacc
    21121 agcgtcgggc ataacgtttg aataaacggc gcttcggtac agtaattcac cacttcggtt
    21181 tttgctccga gcaatttctc aaccacttca accagttgat gattcggtgg gcattcatag
    21241 ccagggatcg gcggatgcag ctcgtcgacc gtcagacgac ccggccagcg ttcgctcacc
    21301 ggagccaatg catcgttgag caaaccatta agttcattga gtgtcatgcc aggctgcgga
    21361 cgaatatcca tatgcaactc acagcaagcg caaatacggt tagaagcgtc gccaccgtga
    21421 atatgcccga ggttgagcgt agggtatggc acggtaaatg cttcgtagtg ataacgttct
    21481 ttcaggttat cgcgcaattg caaaatatgc ccgatggcgt cgtgcattag ttcgatagcg
    21541 ttaattccgc gtgctggatc gctggagtgc cccgactggc cctgaatacg gatggcgtta
    21601 gagatatgac ctttatgtgc gcgtaccggt tgtagtgacg tcggttcgcc aatgatggcg
    21661 caatccgggc gcagggcggt agtttcggca aaataacgcg ctccggccat actggtttct
    21721 tcatcagcag tcgccagaat gtagagcggt tttttcagtt tcgtgacgtc gacatcgcgt
    21781 agcgcatcaa ggataaacgc aaaaaagcct ttcatgtcgg cggtgcctaa gccgtaaagc
    21841 ttgccgtcat gctccgtcag tgtaaacgga tcgcgcgtcc agcgaccgtc atcaaatggc
    21901 accgtatcgg tatgccccgc cagcaacaag ccgccagccc cctgtccgta actggccagc
    21961 atattaaatt tgttgcgagt tcctggaaca ggctgcactt ccacattgaa gcccaaatct
    22021 ttaaaccagt ccgccagcag agtgattaaa tctgcattgc tttgatcgag tgcctcttcc
    22081 gtagcgctta ttgaaggtgt ggcaatcaga gcgcggtaaa tctcgataaa tggcggtaat
    22141 ttgtttttca ttgttgacac acctctggtc atgatagtat caatattcat gcagtattta
    22201 tgaataaaaa tacactaacg ttgagcgtaa taaaacccac cagccgtaag gtgaatgttt
    22261 tacgtttaac ctggcaacca gacataagaa ggtgaatagc cccgatgttg aatacgctga
    22321 ttgtgggtgc cagcggctac gctggcgcag agctagtgac ctatgtaaat cgccatccgc
    22381 atatgaacat aaccgctttg actgtttcag cgcaaagcaa tgatgcggga aagttaatct
    22441 ccgatttgca tccgcagcta aaaggcatcg ttgagctgcc gttgcagccg atgtcggata
    22501 tcagcgagtt tagcccaggg gtggacgtag tgtttctcgc caccgcccat gaagttagcc
    22561 acgatttagc gccgcagttt cttgaagcgg gctgcgtggt gttcgacctt tccggcgcgt
    22621 ttcgtgttaa cgacgccacc ttctatgaaa aatattacgg ctttacccat caatacccgg
    22681 aactgctgga acaggcagcc tacggtctgg cggagtggtg cggtaataaa ttaaaagaag
    22741 cgaatttgat tgcggtgcca ggctgttatc cgacggcggc acagctggcg ctgaaaccgt
    22801 tgattgatgc cgatcttctt gacctcaatc agtggccggt gatcaacgcc accagcggcg
    22861 tgagcggtgc aggacgtaag gcagcgattt caaatagttt ttgtgaagtt agcctgcaac
    22921 cgtatggcgt ctttactcat cgccatcaac cagagatcgc cacacacctc ggtgctgacg
    22981 ttatcttcac cccacatctg ggcaatttcc cgcgcggcat tctcgaaacc attacctgcc
    23041 gcctgaaatc gggtgtgacc caggcgcaag tcgcgcaagc gttacagcag gcgtatgccc
    23101 ataaaccgct ggtgcggctg tatgacaaag gcgttccggc gctgaaaaat gtcgttgggc
    23161 tgccattttg cgatatcggg tttgccgttc agggcgagca tctgattatt gtggcgaccg
    23221 aagacaactt actgaaaggc gcggcggcac aagcggtaca gtgcgccaat attcgtttcg
    23281 gctatgcgga aacgcagtct cttatttaag ggtgcaatga tgaatccatt aattatcaaa
    23341 ctgggcggcg tactgctgga tagtgaagag gcgctggaac gtctgtttag cgcattggtg
    23401 aattatcgtg agtcacatca gcgtccgctg gtgattgtgc acggcggcgg ttgcgtggtg
    23461 gatgagctga tgaaagggct gaatctgccg gtgaaaaaga aaaacggcct gcgggtgacg
    23521 cctgctgatc agatagacat tatcaccgga gcactggcgg gaacggcaaa taaaaccctg
    23581 ttggcatggg cgaagaaaca tcagattgcg gccgtaggtt tgtttctcgg tgacggcgac
    23641 agcgtcaaag tgacccagct tgatgaagag ttaggtcatg ttggactggc gcagccaggt
    23701 tcgcctaagc ttatcaactc cttgctggag aacggttatc tgccggtggt cagctccatt
    23761 ggcgtaacag acgaagggca actgatgaac gtcaatgccg accaggcggc aacggcgctg
    23821 gcggcaacgc tgggcgcgga tctgattttg ctctccgacg tcagcggcat tctcgacggc
    23881 aaagggcaac gcattgccga aatgaccgcc gcgaaagcag aacaactgat tgagcagggc
    23941 attattactg acggcatgat agtgaaagtg aacgcggcgc tggatgcggc ccgcacgctg
    24001 ggccgtccgg tagatatcgc ttcttggcgt tatgcggagc agcttccggc actgtttaac
    24061 ggtatgccga tgggtacgcg gattttagct taagttttgt tagccggagg cgcagctttc
    24121 cggcattgaa tttcaaaata aggaaacaga gttatggcac tttggggcgg gcgttttacc
    24181 caggcagcag atcaacggtt caaacaattc aacgactcac tgcgctttga ttaccgtctg
    24241 gcggagcagg atattgttgg ctctgtggcc tggtccaaag ccttggtaac ggtcggcgtg
    24301 ttaaccgcag aagagcaggc gcaactggaa gaggcgctga acgtgttgct ggaagatgtt
    24361 cgcgccaggc cacaacaaat ccttgaaagc gacgccgaag atatccatag ctgggtggaa
    24421 ggcaaactga tcgacaaagt gggccagtta ggcaaaaagc tgcataccgg gcgtagccgt
    24481 aatgatcagg tagcgactga cctgaaactg tggtgcaaag ataccgttag cgagttactg
    24541 acggctaacc ggcagctgca atcggcgctg gtggaaaccg cacaaaacaa tcaggacgcg
    24601 gtaatgccag gttacactca cctgcaacgc gcccagccgg tgacgttcgc gcactggtgc
    24661 ctggcctatg ttgagatgct ggcgcgtgat gaaagccgtt tgcaggatgc gcttaagcgt
    24721 ctggatgtca gcccattagg ctgtggcgcg ctggcgggaa cggcctatga aatcgaccgt
    24781 gaacagttag caggctggct gggctttgct tcggcgaccc gtaacagtct ggacagcgtt
    24841 tctgaccgtg accatgtgtt ggaactgctt tctgctgccg ctatcggcat ggtgcatctg
    24901 tcgcgttttg ctgaagatct gattttcttt aacaccggcg aagcggggtt tgtggagctt
    24961 tcggaccgcg tgacttccgg ttcatcatta atgccgcaga agaaaaaccc ggatgcgctg
    25021 gagctgattc gcggtaaatg cggccgggtg cagggtgcgt taaccggcat gatgatgacg
    25081 ctgaaaggtt tgccgctggc ttacaacaaa gatatgcagg aagacaaaga aggtctgttc
    25141 gacgcgctcg atacctggct ggactgcctg catatggcgg cgctggtact ggacggcatt
    25201 caggtgaaac gtccgcgttg tcaggaagcg gcgcagcagg ggtatgcgaa cgccactgaa
    25261 ctggcggatt acctggtggc gaaaggcgta cctttccgcg aggcgcacca tattgtgggt
    25321 gaagcggtgg tggaagccat tcgtcagggc aaaccgctgg aagatctgcc actcagtgag
    25381 ttgcagaaat tcagtcaggt gattggcgaa gatgtctatc cgattctgtc gctacaatcg
    25441 tgcctcgaaa agcgtgcggc aaaaggcggc gtctcaccgc agcaggtggc gcaggcgatt
    25501 gcttttgcgc aggctcggtt agagtaattt gtttatattg ctcccctgaa aatggggagt
    25561 ttaccacgcc tacaaagtgc gacacttcgt aggcggataa ggcgtttacg ccgcatcagg
    25621 cagaaatttg tcagcattc
//
LOCUS       DAZXUK010000031        24653 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZXUK010000031 DAZXUK010000000
VERSION     DAZXUK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24653
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.031"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..386)
                     /locus_tag="ACH3L8_001052"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="ACH3L8_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="ACH3L8_001053"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="ACH3L8_001053"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFT4084265.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="ACH3L8_001054"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="ACH3L8_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFT4084266.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="ACH3L8_001055"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="ACH3L8_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFT4084267.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="ACH3L8_001056"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="ACH3L8_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFT4084268.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="ACH3L8_001057"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="ACH3L8_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFT4084269.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="ACH3L8_001058"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="ACH3L8_001058"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFT4084270.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="ACH3L8_001059"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="ACH3L8_001059"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFT4084271.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="ACH3L8_001060"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="ACH3L8_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFT4084272.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="ACH3L8_001061"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="ACH3L8_001061"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFT4084273.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="ACH3L8_001062"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="ACH3L8_001062"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFT4084274.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="ACH3L8_001063"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="ACH3L8_001063"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFT4084275.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="ACH3L8_001064"
     CDS             10748..11212
                     /locus_tag="ACH3L8_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFT4084276.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="ACH3L8_001065"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="ACH3L8_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFT4084277.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFVWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /gene="selO"
                     /locus_tag="ACH3L8_001066"
     CDS             12236..13672
                     /gene="selO"
                     /locus_tag="ACH3L8_001066"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFT4084278.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACH3L8_001067"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACH3L8_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFT4084279.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACH3L8_001068"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACH3L8_001068"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFT4084280.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACH3L8_001069"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACH3L8_001069"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFT4084281.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="ACH3L8_001070"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="ACH3L8_001070"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFT4084282.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACH3L8_001071"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACH3L8_001071"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFT4084283.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="ACH3L8_001072"
     CDS             complement(20505..20798)
                     /locus_tag="ACH3L8_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFT4084284.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="ACH3L8_001073"
     CDS             complement(20795..22084)
                     /locus_tag="ACH3L8_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFT4084285.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="ACH3L8_001074"
     CDS             complement(22140..23078)
                     /locus_tag="ACH3L8_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFT4084286.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="ACH3L8_001075"
     CDS             complement(23098..23862)
                     /locus_tag="ACH3L8_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFT4084287.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24181..>24653
                     /locus_tag="ACH3L8_001076"
     CDS             24181..>24653
                     /locus_tag="ACH3L8_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFT4084288.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT         6103 a         5949 c         6484 g         6117 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgtctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcgggggggg gggcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg
    24001 cgattgctaa aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct
    24061 tgcattcagt gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata
    24121 cacgccgtat tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc
    24181 atgtatcaac gctgttttga taatgccagt gaaacgctgt ttgtcgccgg taaaacgcca
    24241 cggctttcac gttttgcatt tagcgatgat ccaaaatggg agtctggaca tcacgttcat
    24301 gacaatgaaa ccgagctgat ttacgtcaag aaaggggttg caaggtttac catcgattct
    24361 tcgttatatg tcgcacatgc ggatgacatt gtggtgatag aacgcggcag gctgcatgcg
    24421 gtggcctctg acgttaacga tccggcaacg acgtgtacct gtgcgctgta cggctttcag
    24481 tttcaggggg ctgaggaaaa tcagttactg caaccgcatt cttgtccggt aattgccgcg
    24541 gggcagggaa aagaagtcat aaaaacctta tttaatgagc taagtgtgat tttgccgcaa
    24601 agtaaaaata gccaaacatc ttcgttatgg gatgcattag cctatacatt agc
//
LOCUS       DAZXUK010000032        24629 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZXUK010000032 DAZXUK010000000
VERSION     DAZXUK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24629
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.032"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..1934)
                     /locus_tag="ACH3L8_001077"
                     /pseudo
     CDS             complement(1..1934)
                     /locus_tag="ACH3L8_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
     gene            complement(2112..3023)
                     /gene="gsiD"
                     /locus_tag="ACH3L8_001078"
     CDS             complement(2112..3023)
                     /gene="gsiD"
                     /locus_tag="ACH3L8_001078"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFT4084289.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(3026..3946)
                     /gene="gsiC"
                     /locus_tag="ACH3L8_001079"
     CDS             complement(3026..3946)
                     /gene="gsiC"
                     /locus_tag="ACH3L8_001079"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFT4084290.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(3964..5502)
                     /gene="gsiB"
                     /locus_tag="ACH3L8_001080"
     CDS             complement(3964..5502)
                     /gene="gsiB"
                     /locus_tag="ACH3L8_001080"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFT4084291.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(5522..7393)
                     /gene="gsiA"
                     /locus_tag="ACH3L8_001081"
     CDS             complement(5522..7393)
                     /gene="gsiA"
                     /locus_tag="ACH3L8_001081"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFT4084292.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(7380..8345)
                     /gene="iaaA"
                     /locus_tag="ACH3L8_001082"
     CDS             complement(7380..8345)
                     /gene="iaaA"
                     /locus_tag="ACH3L8_001082"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFT4084293.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            8549..9784
                     /gene="moeA"
                     /locus_tag="ACH3L8_001083"
     CDS             8549..9784
                     /gene="moeA"
                     /locus_tag="ACH3L8_001083"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFT4084294.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            9784..10533
                     /gene="moeB"
                     /locus_tag="ACH3L8_001084"
     CDS             9784..10533
                     /gene="moeB"
                     /locus_tag="ACH3L8_001084"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFT4084295.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(10712..11374)
                     /gene="fsa"
                     /locus_tag="ACH3L8_001085"
     CDS             complement(10712..11374)
                     /gene="fsa"
                     /locus_tag="ACH3L8_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFT4084296.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            11505..12404
                     /locus_tag="ACH3L8_001086"
     CDS             11505..12404
                     /locus_tag="ACH3L8_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFT4084297.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            12410..14842
                     /locus_tag="ACH3L8_001087"
     CDS             12410..14842
                     /locus_tag="ACH3L8_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFT4084298.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            14988..15803
                     /gene="ybiV"
                     /locus_tag="ACH3L8_001088"
     CDS             14988..15803
                     /gene="ybiV"
                     /locus_tag="ACH3L8_001088"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFT4084299.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            15955..17220
                     /locus_tag="ACH3L8_001089"
     CDS             15955..17220
                     /locus_tag="ACH3L8_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFT4084300.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRLLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(17461..19053)
                     /locus_tag="ACH3L8_001090"
     CDS             complement(17461..19053)
                     /locus_tag="ACH3L8_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFT4084301.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            19272..20192
                     /gene="ldtB"
                     /locus_tag="ACH3L8_001091"
     CDS             19272..20192
                     /gene="ldtB"
                     /locus_tag="ACH3L8_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFT4084302.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(20250..21367)
                     /locus_tag="ACH3L8_001092"
                     /pseudo
     CDS             complement(20250..21367)
                     /locus_tag="ACH3L8_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
     gene            complement(21364..21831)
                     /gene="mntR"
                     /locus_tag="ACH3L8_001093"
     CDS             complement(21364..21831)
                     /gene="mntR"
                     /locus_tag="ACH3L8_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFT4084303.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            22017..22145
                     /gene="mntS"
                     /locus_tag="ACH3L8_001094"
     CDS             22017..22145
                     /gene="mntS"
                     /locus_tag="ACH3L8_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFT4084304.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            22417..24000
                     /gene="opgE"
                     /locus_tag="ACH3L8_001095"
     CDS             22417..24000
                     /gene="opgE"
                     /locus_tag="ACH3L8_001095"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFT4084305.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(24048..24563)
                     /gene="ompX"
                     /locus_tag="ACH3L8_001096"
     CDS             complement(24048..24563)
                     /gene="ompX"
                     /locus_tag="ACH3L8_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFT4084306.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
BASE COUNT         6144 a         6664 c         6022 g         5799 t
ORIGIN      
        1 aggccatttt ctttcatgat ggtggtaaaa cgctcatgaa agtgcggttc gtttaactgc
       61 cagggcgaaa cattaatatg cagtaagaaa tcatccttca gcgacgcacg ttgactaaaa
      121 tgtctgaatt aacccattgc cgttttaata atctgataac ccagctcatt gataaacccg
      181 atatcttcag caatggtaat gaacttcagc ggtgaaatga tcccccgctc cgcagaaacc
      241 caacggcaca acgcctccgc gctcagaatg gcgcgggtgt taatatcaac gatcggttga
      301 tagacgagga atagttcgcc aatttccatt gcatggcgaa tattacgcgc aatccagata
      361 ttctcttcta tttcgttgag tttcgcgtca tcacagaaaa taaactcgga gaacatatgg
      421 tcacggatac tggaaatcgc cagtttcagt ttttctgcgt aactccaggc gttttccccc
      481 tgcaaaggtt cacaaatggc agacttatgg atgcgcagat tgcggtagcc aaagctgctg
      541 tcaaaatcgt ttaccatcgc tattttacgg gtaaatgtct gtacattttc ccgacacaga
      601 agtaaataga gatcggcatt gaatgtcacg atatcgcagc attgcgcata tttctcggca
      661 attcgctgac acaatttcct gacgatttta ttgcgttcat caacaccaaa caccgcctcc
      721 acggcttcca gaccacacag tttgatgatc cccacagcca gatactgatt attgttggac
      781 tcaataatcg ccatattttt aataaaaccg ctttcctgat cgtaagagat cagtgagagc
      841 aatttatttg accgttcaat ttcggcatct ttcatttccg cgatcgaatt tttgatgctg
      901 ataatctctt tcgcatcgcc ttcgcgaatt ttcagagcct cgatctgatc cgttgagtcg
      961 ggcaaaaacg ccagcgtatt aaccagctcg gtcatttcag aaaattgccg acggaacaca
     1021 cgagtggtca tgaaatatac caccagcaac acgataataa tgaacgcaaa cggtagataa
     1081 aaaagtttat ctgatgcgtt caataaatta ccgccaggat aataaaaata gatttcgaac
     1141 aatgcattat ttggcactgc tgatgtcttt gcaatatact ccgtatcgac agaatcaata
     1201 atattataat taccttcgct attataaata atcagttttt cacgagcgag ttgattcttg
     1261 gcaatgatct catgaccttg tttcaaaaat agaacttcat cattagcgct gatttgatca
     1321 agatactcgt tcagataccg ccagtttaaa tcgacatgaa ttttatctac cgctaacaaa
     1381 tcgtgatctt tgccgttgat acgtagcgta aaagagatac attttttacc cggacgatca
     1441 aagcaattgt atgccggatt ccagctgttg ttagagtcat ctgaagccac cgaccacggt
     1501 cgtttcgatg ggttaaaatc ggcaaaaata acccgccctg actcgtcgac aagcggtcca
     1561 tttacttgtt cctgatgata aacaacgatt ttatcattcg ccctccgcga aaagagataa
     1621 tatttattat catcaagtac cagtcccacc gagtctaaaa atggcatcat ttccattttc
     1681 agaatcacgg cgcgttgaat ctcctctgta ttttttaata aatacagtga gtcctttaat
     1741 gtcagggccg aacgttcaat atctttaaag aaatagccaa cgtttttctg aataagttca
     1801 aagcggctat cggcacgttt taccgcgcgt tccgatacca gttgatagtt aatcatgaaa
     1861 aaaagaccaa taaatgacag tgctgccaat aaaaataaaa ttatcagcct tatcttttcg
     1921 tataaactca gcatgttaat acatttaaca aaataactat ctgaacgctg tgccgctgca
     1981 aaaatagtcc cttgctgcgg ctttttaacc aaattacccg attctacaaa atcagcagaa
     2041 agttacaaca cttgcttagg gcaataattt cctgtgaggt gattaccctt tcaagcaata
     2101 ttcaaacgta actatccttt aattttcgga tccagcgcat cgcgtaaacc atcgcccaac
     2161 aaattgaacg ccagtacggt cagaaaaata gccagggccg gaaaaacagc gacatgcggc
     2221 gcgataacca tatccgctcg tgcctcattg agcattgctc cccactctgg tgtcggcggc
     2281 tgcgcaccga ggccgagaaa tgacaggctg gcggcagaga taatcgaggt gccaatgcgc
     2341 atggtgaaaa acaccacgat agaagagacg gtcccaggca ggatatgacg caataaaatg
     2401 gtcatatcgc tggcaccaat actgcgtgct gactcaataa aggtttgctg tttcaacacc
     2461 agcgtgttgc cgcgcaccag gcgggcaaac gcggggatgg aaaaaatggc gactgcgata
     2521 atcacgttag caatgccgct tcccaacacc gcaacaacag cgatcgccag taaaataccc
     2581 gggaaggcaa acagcacatc gcaaatgcgc atgatcagcc gatcccacca gccttcataa
     2641 tatccagcca acaagcccag caacgtccct atcgccacac cgataaacac ggcaaacacg
     2701 cccgccgcca gcgagatttg cgcaccaacc aggacacggc tgaaaatgtc gcgccccagt
     2761 gaatcgacgc caaaccagtg ctgcaaagaa ggtccgttat tcagattgtc ataatcaaaa
     2821 taattttcgg cgtcataggg agcgatccag cgtgcaaaaa tggccaccac aatcaataaa
     2881 ataacgaata atgcggcggt catcgccata tgctgacggc gaaatcgtcg ccagaattca
     2941 tgccacggtg tacgtacctg gtcaggtttg accagtggca tggcgtttaa caccgcctga
     3001 cgtcgccagt taaatagtcg catccttact tgtacctgat agccgggtta atggcggcgt
     3061 aaagcacatc caccactaag ttgataagaa taaattccag cgagaaaagc agaatttccg
     3121 cctgaatcac cgggtaatcg cgcatttcta cagagtcaac gagtaagcgt ccaagtccgg
     3181 gccagttgaa aactttctcc accacgatag aaccaccgag caaaaagcca aactgtaagc
     3241 ccatcatggt cactaccggg atcatcgcgt tgcgtagccc gtgtttgagg acaacccagg
     3301 tttcgctcac ccctttcgcc ctcgcggtac gcatataatc ttcgcttaaa acgtcgacaa
     3361 atgacgcacg ggtaaagcgc gccatcacgg cggccactgc cgcgccgagc gtcagggagg
     3421 gtaaaatgta gtgctgccaa ctgtctgctc ccacagtagg cagccagccc agttcaacgg
     3481 agaatacctg aattaaaaac atccccagcg caaatgccgg aaacgagatc cccgacaccg
     3541 caatggtcat actcaatcga tccggccaac gattacgcca gacggcggcg ataattcccg
     3601 ccgccatacc aaatatcacc gcccagacca tactggttat ggtcagccat agcgttggca
     3661 taaagcggct ggcaatctca tcggcaaccg gacgacgcga caccatcgac aggccaaaat
     3721 ccccctgcac agcattgctg atatagtgcc agaactggtg atacagcggc tgatccaacc
     3781 ccagctgctg acgcaccagt tctataacct gcgcatcagc ttcgggcccg gcaatcaatc
     3841 gcgccggatc gccgggcagc atgtggacaa ataaaaacac cagcaccgag acgataaaca
     3901 gcgtcggaat caaccccagt aatcgtttga taacgtaata aagcattcca ctccctgcgt
     3961 tgcttattgc aaatccgcgt cttcaaagct gaagccggta tctggcatga tccaaaaacc
     4021 ggtcaggttt ttactgtgtg ccgacaccag tttttctacc accagcggga tccacggcga
     4081 ttcttgccag atgatatcct gcgccgcctt atataagcgg gtcttttccg ccggatcatt
     4141 cgttttcagt gcctgagcca ggaagtcatc cacctgttta ttgctgtaaa acgcggtatt
     4201 aaacagcgtc ggtggccagt tttgcgaggc aaacagcggc gatagtgccc agtcggcttc
     4261 gccggttgaa gccgaccagc cagtgtagaa catccgcacg ccactctctt tttgcccttt
     4321 accttcaact tctgccgccc gctgtccggc atccatcgcg gtcacctggg ctttaatccc
     4381 gacctgcgct aactgctgct gggtaaattg cagcactttc tgcgcggtgc tgtggttatg
     4441 tgacgaccac agcgtggtac tgaaaccgtt gggatatccc gcctctttca gtaattcgcg
     4501 cgctttcact ggatcgtaag gccacggttt ataactttgc gcgtaggcga tacttggcgg
     4561 taccacacca gtagctggag ttgcatagcc cgcaaaggca actttcacca gcgccggacg
     4621 gttaatggcg taattcagcg cctcacggac cttcgggtta tcgaacggct tttgcgtcac
     4681 gttcatactg atataacgct gcataattga cggactggcc atcaactcga tatttttgtt
     4741 tttctccagc agtgtggcct gctcgtaggg aatggggaaa gcaaactgcg cttcaccggt
     4801 ttgcagcatt gccgcgcggg tgttgttatc cgccaccgga cgccaggtta tgctgtccag
     4861 tttgggcaat cctggctgcc agtaacccgc gaattttttc accttcacaa aatcggtctg
     4921 attccaggta tccagttcat acggtccggt tcccaccgga tgaaaaccaa tctccttgcc
     4981 atatttttcc agcgctgtcg gtgaaatcat cgcggtcgcc ggatgggcaa gaatattaat
     5041 aaacgctgag aacggctgtt tgagggtaat ctttaccgtt gtcggatcga tcgcctccgt
     5101 tttggcaata ttcttataca ggttatagcg tttaagatga ttcgccggat cgctggcccg
     5161 gtccagattc gcttttaccg ccactgcgtt gaaatcggtg ccatcctgga atttaattcc
     5221 ttcccgcaat ttcacggtgt aagtaatgcc gtcatcggaa acggtataac tctccgccag
     5281 cacgtttttc agtttcatct ctttatccag accgaacagc ccctggtaaa acgatttcgc
     5341 tacggcctga gataacgtgt cattcgcgtc atacggatcg agcgtggtga aattcgatcc
     5401 taccgccacc accacatctt tggcagcgaa tgcacaagat gccatcaacg ctgtcgcaat
     5461 gcccagcgcc actaacccac tacggtgtac agctcttgcc attttattct ccgcctgaat
     5521 gttatctacg catgaatgcg tattctgatt gtggttgtgc gacgtaatgc cccggcccga
     5581 cgcattgcaa cgagacggct gccacctctt cgccacgcag atgaatattg ctgggaagat
     5641 cgtccgacag cagtacacgc tgcggtcgtt gtcgggacgg ttcagcgacc ggaactgccg
     5701 ccagtaattt acgcgtataa ggatgctgcg ggttttcgaa gaccgcgcac cgtgaaccaa
     5761 tttcaacaat ttgcccgaga tacatcaccg ccacacgatg actaatccgc tctaccacag
     5821 ccatatcgtg ggagataaac agatacgcaa tgccgaaatc acgctggaga tcgagcaaca
     5881 agttgataat ctgcccgcga atagaaacat ccagcgccga aacggcttcg tcggcaatga
     5941 tcacttttgg attcaatgcc aacgcgcgag caatgcagat gcgctggcgc tgaccgccgg
     6001 aaaactcatg cgggtaacgc caggcatgtt caggtaacag gcccacgcgc tccagcaacc
     6061 acgcaacgcg tgcagccgcg tctttacctg gcaataaacc gtgtacacgc agcggttcga
     6121 taatcgaatc accgatggtc tgacgtgggt ccagcgaagc gtaagggtcc tgaaaaataa
     6181 actgaatatc ccggcgcaat gcctgaagtt tgccaggtga caaggtatcg attcgctgac
     6241 cgttaaagat aatttcgccg ccctgcgatt cgaccaggcg cagcaacgcc cgcccggtag
     6301 tggatttacc gctgccagac tcgcccacca gcgatagcgt ttcgccaggc cagagatcaa
     6361 aactgacttt ctcaacggca tgcacttccc gcgttacgcg attcaacaaa ccgctgcgca
     6421 aagggaaacg ggtgacaaga ttacgcaccc gtaaaacagg ttcgccatcc accaccgttt
     6481 tctgctcgat ggggggttcc tgtttcgctg gatgttcaag cgatatcaat gggaaacgtc
     6541 ggggataatc taaccctttc atcgcaccaa gttgcggaac agcagctaac agcgcacggg
     6601 tgtaaggatg ttgcggtgca tgaaaaatct gttcgacggt acccgtttcc accgcctcgc
     6661 cctgatacat caccagtacc cgatcggcaa tctctgccac cacgcccata tcgtgagtga
     6721 taaagataac gcccatcgac atctcttttt gcaatacttt gattaattgc aggatctgcg
     6781 cctgaatagt gacatccagc gcggtggttg gctcatcggc aatcagcacc gccgggcggc
     6841 atgacagcgc catcgcaatc atcactcgct ggcgcatccc gccagagagt tgatgcgaat
     6901 aacgtgaaag aatggtttgt gcctccggaa tgcgtacctg atccagcatc cgcttcgcct
     6961 cgaccatcgc ttcttcacga ctggcgttct gatgcagacg aattgattcg gcaatctgtt
     7021 cacccacagt aaatactggg ttcagcgatg tcatcggctc ctgaaatatc atcgccatat
     7081 ccgcaccgcg cacatggcgc atttgtgcag cgctctgctc gctaagttca atcacatcac
     7141 gactgcgccg ccgcaacagc attttatcgc actgtactaa accgcccgcc tgttccaaca
     7201 gacgcatcaa tgccagcgca gtcactgact taccggagcc ggattcgcct acaattgcca
     7261 gcgtctcacc gcgttgcaga ctaaaagaga gattgcggac cgcagctatt ttctgctggt
     7321 cctgcataaa ggcaatatta agattttcaa ccgccagcac attaccggca tcaagttcat
     7381 cactgtgtgg caacggtgtc ccctttttca cggtagatac cggtggttgg cgtatcgcct
     7441 gcgtagcccc aggcgcgata cattccttcg gtgttaaacg gtagcgcgac attcccttca
     7501 tggtcgatag cgattaagcc accgctaccg ccaagcgcag ggagtttttc cattactacc
     7561 cgctcgcagg cttccgcgag acttaatccg ccgtaatcca ttaacgcggc gatgtcatat
     7621 gccgccagcg cgcggatgaa gacttcgcct gtgccggtac aagaaaccgc cacactggcg
     7681 ttattggcgt agcatccggc acccactaag ggactatcgc caactcgtcc gggtaattta
     7741 ttggtcattc cgcctgtgga cgtggctgcc gccagattgc cgtctaaatc caacgccacg
     7801 gcccccacgg tgcccatttt ctgtttttca tccagtggcg caccgctatg gtcgaggact
     7861 gttgcccctt cctcgcgcgc tgccagtagt tgttcataac gcaaaggcgt ggagaaaatc
     7921 tccggtgaga cgcgctccat gccatgagca aacgcaaaat tttctgcccc ttcgccaatc
     7981 atcatcacat gcgggctttg ctccatcacc agccgggcgg caagaaccgg attacgcaga
     8041 tgactaacgc ccgccaccgc accggctttc agggtgttac catccatcac acaggcgtcc
     8101 agttcatggg tttcatcacg cgtaaagaca gcgccaattc cggcgttaaa cagtggacac
     8161 tcttccagca gacgcaccgc ttccgtcacc acatccagcg cactttcgcc ggcttccagc
     8221 attttctgcc cggtttcaac aatggcagac aacgcctcga tgtagcgtaa ttcctgttgc
     8281 agactcatct gcgcgcggct aattgcacct gcgccaccat gaattgcaat gactgctttg
     8341 cccatagtat tcgtccatta agggtggctc gccacttttg ctataaatat cagaattttt
     8401 atgaattact cattcaattt ttgaatatag aaagatggat atggcatgta aaggcaggag
     8461 ccaacgctac gcccgaggtg gcatacagtc tatggcgttt ttctgacata ataggcaaat
     8521 tcgattttgc ctccgcagga gtgttttcat ggaatttacc accggattga tgtcgctcga
     8581 caccgcgctt aatgagatgc tttctcgcgt taccccactg accacccagg aaacgctgcc
     8641 actggttcag tgttttggtc gtattctggc gagcgatgtc gtttcgccac tggatgtccc
     8701 ggggtttgat aactccgcta tggacggcta cgcggtgcgt ttagccgata ttgcctccgg
     8761 gcaaccgctg cccgttgccg gtaaatcctt tgccggtcag ccataccatg gtgaatggcc
     8821 tgcgggtacc tgcattcgta ttatgaccgg tgcgccggtg ccggaaggct gcgaagcggt
     8881 ggtgatgcag gagcagactg aacaaacgga caatggcgtg cgttttactg ctgaagtgcg
     8941 tagcgggcaa aatattcgcc gtcgcggtga agatatctct gcaggtgcgg ttgttttccc
     9001 ggcgggaact cgcctgacta ccgcagagct gccagtaatt gcttcactgg ggattgccga
     9061 agttccggtg attcgtaaag tgcgtgtagc gcttttttct accggtgatg aactccagtt
     9121 gcccggtcag ccgctgggcg acggccaaat ctacgacacc aaccgtctcg ccgtacacct
     9181 gatgttagaa cagctgggtt gcgaggtaat caacttaggg attatccgcg acgatccaca
     9241 tgccctgcgc gccgcattta ttgaagccga cagccaggcg gatgtagtga tcagttccgg
     9301 cggtgtttca gtgggtgaag cggattacac caaaatgatt cttgaagagc tgggggagat
     9361 cgccttctgg aagctggcga ttaaaccagg taaaccgttc gcgttcggta aactcagcaa
     9421 tagctggttc tgcggcctgc cgggcaaccc ggtttcagcg acgctgacct tctatcaact
     9481 ggtacagcct ttgctggcaa aactaagcgg caacaccgcc agcggcctgc ccgcgcgcca
     9541 gcgcgtacgc acagcgtccc gcctgaaaaa aacgccagga cgtcttgatt tccagcgcgg
     9601 cgtgctgcaa cgcaacgccg acggtgaact tgaagtgacg accaccggac atcagggttc
     9661 acatatattt agctccttta gtctcggcaa ctgctttatc gtgctggaac gcgatcgcgg
     9721 caatgtggac gtgcgcgaat gggtggaagt agaaccgttt aacgcgttgt tcggaggcct
     9781 gtaatggcgg aactcagcga tcaggagatg ctgcgctaca accggcaaat cattctgcgc
     9841 ggttttgatt ttgacggtca ggaggcgcta aaagattctc gtgtgctggt agtcggcctc
     9901 ggtggactcg gctgcgcagc ttcgcagtat ctggcaagcg ccggtgtcgg caacctgacg
     9961 ctgctcgact tcgacacggt ttcgctctcg aatctgcaac gccagacact gcacagtgat
    10021 gccacggtcg ggcaaccgaa ggtggaatcc gcccgtgacg ccctgacgcg gatcaaccca
    10081 catatcgcga ttacgccagt caatgcactg ctagatgacg cagaacttgc agcattgatt
    10141 gctgaacacg atctggtgct cgactgtacg gataacgttg cggtacgtaa tcaactgaac
    10201 gcaggctgtt ttgccgcgaa ggtgccgctg gtttccggcg cggcaattcg tatggaaggg
    10261 caaatcacgg tcttcactta tcaggacggc gaaccgtgct atcgctgcct tagccgtttg
    10321 tttggcgaga acgcgttaac ctgcgtagaa gcaggcgtga tggcaccgtt gataggcgta
    10381 attggttcgt tgcaggcaat ggaagcgatc aaactgctgg caggttatgg caaaccggcc
    10441 agcgggaaaa tcgtcatgta cgatgcgatg acctgtcagt ttcgcgaaat gaaactgatg
    10501 cgcaatccgg ggtgtgaggt gtgcgggcag taattgccac ctccaagcct gataagacgc
    10561 gacaagcgtc gcatcaggca tttgctccag acgccagatg cggcgtgaac gccttatctg
    10621 gcctacggtt cgggcacaga tttccaagcg cggcaggcat cggtgcactc cgccacgtca
    10681 tcagcaattt atgaggatgc agaacgtccg gttaaatcga cgttctgcca aacgctccct
    10741 gccagtcctg ctcaaacttc gtcacagcgg cttccaccgc cggatagcta atcatctgtt
    10801 gtgccacatc cagtggcaga gtaattgatt cacatccggc cagtaagcag tccagcgcct
    10861 gacgcggggt tttgaaactc gctgccagca ctttcgcctg cggcgcatgc attttcaata
    10921 actggtgtaa gtcggtcaca gtctgaatgc cgctaccgcc ctgagcatca atacgattaa
    10981 cgtaaggcgc aacatattcc gcacctgcca gcgccgacag cagcccttgt gccgcgccat
    11041 ataccgcggt tcccagcgtc ggaatccctt ccgcttttaa catcttaatc gctgccagcc
    11101 cctcggctgt caccggaact ttcaccacga tatccgcaat aatagaacgc agcttaagcg
    11161 cgtcattcac catcccttca gcagtggtag ccattacctg ggcaaacaga cgcccctgac
    11221 cgcccatcgc ttcatgaagt tgcggaagca caacatccag cggtttttta cccgcggcga
    11281 taatgcttgg gttagtggtc acacccgcca gcggaaaaat acgtgacagc gccttcaccg
    11341 caacaacgtc tgaagtatcc agatacagtt ccataaccat cctcaaaatg tactttttgt
    11401 ctgaatgtag actatcacgg gaaaactctc tgaacagttg acgcacatca atctaacttt
    11461 cattcgaaag tattttaatg tttgaacgaa acagagaggc aactatgatt ttcaatattc
    11521 agcgctactc gacccatgat ggccccggta tccgcacggt cgtatttctt aaaggctgtt
    11581 cgctgggctg ccgctggtgt cagaacccgg aaagccgcgc ccgtacgcag gatctgctgt
    11641 atgacgcacg actgtgtctg gaaggctgcg agctgtgcgc taaggccgcg ccggaagtga
    11701 ttgagcgcgc gctgaatggt ttgcttattc atcgggaaaa gttaaccccg gagcatctga
    11761 cggcgttaac cgactgctgt ccgacacagg cgttaaccgt gtgtggtgaa gtgaaaagcg
    11821 ttgaggagat catggcgacc gttctgcgcg ataaaccgtt ttacgatcgc agcggcggcg
    11881 gcttaacgct ttcgggtggt gaacccttca tgcagccgga aatggcgacg gcgctgctgc
    11941 aagcctgcca cgaggcaggc attcatactg cggtagaaac ctgtctgcat gtgccgtgga
    12001 aatatatcgc cccttctctg ccctatatcg atctgtttct tgccgattta aaacacgttg
    12061 ccgacgcgcc gtttaaacag tggaccgacg gtaacgccgc cagggtgctg gataacctga
    12121 aaaaactcgc cgcagcgggc aaaaaaatca ttatccgcgt gccgctgatt cagggcttta
    12181 atgccgacga aacctctgta aaagccatta ccgattttgc cgccgacgag ctgcacgttg
    12241 gcgaaattca ttttctgccc taccacacgc tgggcatcaa caaatatcac ttacttaatc
    12301 tgccctatga cgccccggaa aaaccgcttg atgcgccaga gctgctcgat tttgcccagc
    12361 agtatgcctg ccagaaaggg ttaaccgcga ccttacgagg ataacaacca tgaccacact
    12421 gaaactggac acgctcagcg accgcattaa agcgcacaaa aatgcgctgg tgcatattgt
    12481 gaagccgcca gtctgtaccg agcgcgcgca gcactatacc gagatgtatc aacaacatct
    12541 cgataagccg atcccggtac gtcgcgcgct ggcactggcg catcacctgg cgaatcgcac
    12601 catctggatc aaacacgatg agttgatcat tggcaaccag gcaagcgaag ttcgcgccgc
    12661 gccgatcttc ccggaatata ctgtctcgtg gatcgaaaaa gagattgatg atctggcaga
    12721 tcgtcccggt gctggctttg cggtgagcga agagaacaaa cgtgttctgc atgaagtgtg
    12781 cccgtggtgg cgcggtcaga cggtacagga tcgctgctat ggcatgttta ccgatgagca
    12841 aaaaggtctg ctggcgaccg gcatcattaa agcggaaggc aatatgacct ccggcgatgc
    12901 gcacctggcg gtcaatttcc cgctgctgct ggaaaaaggg cttgatggtc tgcgtgagaa
    12961 agtagcggaa cgtcgctcgc gcatcaatct gacggtgctg gaagatttgc acggcgaaca
    13021 gttcctgaaa gcgattgata tcatgctggt ggcagtcagt gaacacattg aacgtttcgc
    13081 tgccctggcg cgtgcaatgg ccgcgaccga aacccgcgaa agccgtcgcg atgaactgct
    13141 ggcgatggca gaaaactgcg atcttatcgc ccaccagccg ccgcagactt tctggcaggc
    13201 gctgcaactg tgttacttca tccagttgat tttgcagatt gaatctaacg gtcactcggt
    13261 gtcgtttggt cgtatggatc agtatctcta cccgtactat cgccgcgacg ttgaactgaa
    13321 ccagacgctg gatcgcgaac acgccatcga attgctgcat agctgctggt tgaaattgct
    13381 ggaagtgaac aaaatccgct ccggctccca ctcaaaagcc tctgcgggaa gtccgctgta
    13441 tcagaacgtc accattggcg gacaaaatct ggttgatggt caaccaatgg acgcggtgaa
    13501 tccactctct tacgcgatcc tcgaatcctg cggtcgcctg cgttccactc agcctaacct
    13561 cagcgtgcgt taccatgcag gaatgagtaa cgatttcctc gacgcctgcg tacatgtgat
    13621 ccgctgcggc ttcgggatgc cggcattcaa caacgacgaa atcgtgatcc cggaatttat
    13681 taaactcggt attgaaccgc aggacgctta tgactacgca gcgattggtt gtatagaaac
    13741 cgccgtcggt ggcaaatggg gctatcgctg taccggcatg agctttatca acttcgcccg
    13801 cgtgatgctg gcggcgctgg aaggcgggcg tgatgccacc agcggcaaag tgttcctgcc
    13861 acaagaaaaa gcgttgtcgg caggtaactt caacaacttc gatgaagtga tggacgcgtg
    13921 ggatacgcaa atccgttact acacgcgtaa atcaatcgaa atcgaatatg tcgtcgacac
    13981 catgctggaa gagaacgtgc acgatattct ctgctcggcg ctggtggatg actgtattga
    14041 gcgagcgaaa agtatcaagc aaggcggcgc gaaatatgac tgggtttctg gcctgcaggt
    14101 cggtattgcc aacctcggca acagcctggc ggcagtgaag aaactggtgt ttgaacaggg
    14161 cgcgattggt cagcaacagc tggctgccgc actggcggat gacttcgatg gcctgactca
    14221 tgagcaactg cgtcagcggc tgattaacgg tgcgccgaag tacggcaacg acgatgatac
    14281 cgtcgatacg ctgctggctc gcgcttatca gacctatatc gacgaactga aacagtacca
    14341 taacccacgc tacggtcgtg gtccggttgg cggcaactat tacgcgggta cgtcgtctat
    14401 ctccgctaac gtaccgtttg gcgcgcaaac tatggcaacg ccggacgggc gtaaagccca
    14461 caccccgctg gcagaaggcg ctagtccggc ctctggtact gaccatctcg gccctactgc
    14521 ggtcattggc tcagtgggta aactgcctac ggcagcgatt ctcggcggcg tgttgctcaa
    14581 ccagaaactg aatccggcaa cgctggagaa cgaatctgac aagcagaaac tgatgatcct
    14641 gctgcgtacc ttctttgaag tgcataaagg ctggcatatt cagtacaaca tcgtttcccg
    14701 cgaaacgctg ctggaggcga aaaaacatcc ggatcagtac cgcgatctgg tagtgcgtgt
    14761 cgcgggttat tccgccttct tcaccgcgct ctctccagac gctcaggacg atatcatcgc
    14821 ccgtactgaa catatgctgt aatctcttcg gcccggctgc ggcgtcgccg ggctaaatca
    14881 ttcacatcat caatttcatc cttactttca ttcgaaatat aatttgtgct ctgcgtcaca
    14941 ttgttattac actgttacgg tttcattttc gagccaggag cacacctatg agcgtaaaag
    15001 ttatcgtcac agatatggac ggtacttttc ttaacgacgc caaaacgtac aaccaaccac
    15061 gttttatggc gcaatatcag gaactgaaaa agcgcggcat taagttcgtt gtcgccagcg
    15121 gtaatcagta ttaccagctt atttcgttct ttcctgagct aaaggatgag atctcttttg
    15181 tcgcggaaaa cggcgcactg gtttacgaac atggcaagca actgttccac ggcgaactga
    15241 cccgacatga atcgcggatt gttattggcg agttgctaaa agataagcaa ctcaattttg
    15301 tcgcctgcgg tctgcaaagt gcatatgtca gcgaaaatgc ccccgaagca tttgtcgcac
    15361 tgatggcaaa acactaccat cgcctgaaac ctgtaaaaga ttatcaggag attgacgacg
    15421 tactgttcaa gttttcgctc aacctgccgg atgaacaaat cccgttagtg atcgacaaac
    15481 tacacatagc gctggatggc attatgaaac ccgtcaccag tggttttggc tttatcgacc
    15541 tgattattcc cggtctacat aaagcaaacg gtatttcgcg gttactgaaa cgctgggatc
    15601 tgtcaccgca aaatgtggta gcgattggcg acagcggtaa cgatgcggag atgctgaaaa
    15661 tggcgcgtta ttcctttgcg atgggcaatg ctgcggaaaa cattaaacaa atcgcccgtt
    15721 acgctaccga tgataataat catgaaggcg cgctgaatgt gattcaggct gtgctggata
    15781 acacatcccc ttttaacagc tgacctctct accggagcca tgcgctccgg ttttcccttc
    15841 tttttacatc ctgtacatca catcaaatca ttaaacttgc attaacttat ttataacttg
    15901 tattgtcaca tgcaaattag catagtttcg aatggaagtt aatgcaaggt ggttatggct
    15961 tctactttta ccagcgacac attgcctgcc gatcacaaag cagctatccg tcagatgaag
    16021 cacgcgctgc gggcgcagct tggcgatgtc cagcagatct ttaatcagct aagcgatgac
    16081 attgccacgc gagtggctga aatcaacgca ctcaaagcac agggcgatgc cgtctggccg
    16141 gtgctgtctt atgccgatat caaagcaggt catgttactg cagagcagcg cgaacagatt
    16201 aaacgtcgcg gttgtgcggt gataaaaggc catttccccc gcgaacaagc gctaggctgg
    16261 gatcagtcga tgctggacta tctgaaccgc aaccgctttg acgaggtcta caaaggcccc
    16321 ggcgataatt tcttcgggac gctcagcgct tcacgtcccg agatttaccc catctactgg
    16381 tcgcaggcgc aaatgcaggc ccgccagagt gaagaaatgg cgaatgcgca gtcgtttctc
    16441 aatcgtctgt ggacatttga aagtgatgga aagcaatggt ttaacccgga tgtgagcgtc
    16501 atctaccctg accgtatccg cctccttccg cccggaacga cctccaaagg tcttggagcg
    16561 cataccgact ccggggcgct ggaacgctgg ctgcttccag cgtatcagca cgttttcgcc
    16621 aacgtcttta atggcaatct ggcgaaatac gatccctggc atgcggcaca tcgtacggaa
    16681 gttgaagagt acacggtgga caacaccacc aaatgttccg tgtttcggac attccagggc
    16741 tggactgcgc tctcggatat gctgcctggt caggggttgc tgcacgtcgt gcccattcct
    16801 gaagccatgg cgtacgtact gttacgtccg ctgcttgatg atgtgccgga ggatgaactg
    16861 tgcggcgtag cgcccggaag agtgttgccg gtatcagagc aatggcatcc actgttaatt
    16921 gaggcgttaa ccagcattcc aaaactcgag gccggagact ccgtctggtg gcactgcgac
    16981 gtcatccatt ccgttgcccc cgttgaaaat caacagggtt ggggcaacgt gatgtacatt
    17041 cctgcggcac cgatgtgcga gaaaaatctt gcctacgcgc acaaggtgaa ggccgcactg
    17101 gaaaaaggcg catcgccggg cgacttcccg cgcgaggact atgaaacaaa ctgggaagga
    17161 cgctttacgc ttgccgacct caacattcac ggtaagcgag cgttgggcat ggatgtttga
    17221 gagttaatgg aagtgaattg atgtgggatt aagaaaatcg attcgacgtt actcagattg
    17281 ctgacaagtg cgctttctta atgcctgatg cggcgttaac gccttatccg gcctacaaag
    17341 tcctgcagat tcaatatatt acaggtactg cgtaggcctg ataagcgtag cgcatcaggc
    17401 tattttgcat ttgtcatcag ccgtcgtcac cccgacagta tcggggtgac ggataatcac
    17461 ttactcgatc cctttactac gcaggtaatc ttcgtaatta ccgctaaagt cgatcacgcg
    17521 ttccggggtg atttccagaa tgcgggtcgc cagggagctt acgaactcac ggtcgtgtga
    17581 aacaaagatc agcgtgccct gatacagttc cagcgccatg ttcagtgact cgatggattc
    17641 catatccagg tggttggtcg gttcgtccat gatcagaatg ttcggcttct gcatcattaa
    17701 cttaccaaac agcatccgcc ctttttcccc accggaaagc actttagctg gctttttgat
    17761 gtcgtcctgg ctgaacagca aacgaccgag aatgctgcgt accgcctgct cgtcatcgcc
    17821 ttcctgcttc cactgactca tccattcgaa cacggtcaga tcattttcaa actcatattc
    17881 gtggtcctgg gcgtagtaac caatgcgcgc gttttcagac cattttacgg tgccgctgtc
    17941 cggttgcaga tcgcccacca gcgttttcag cagcgttgat ttaccgacgc cgttggtacc
    18001 cagtaccgca agtttttcac cgacctccag cagcaggttg agatttttaa acagtggacc
    18061 gttatcaaac cctttggtca gaccttccac ttccagcgcg ttacggaaca gtttcttatc
    18121 ctgttcaaaa cggataaacg ggttctgacg gctggaggct ttcacctctt ccagtttgat
    18181 tttatcaatc tggcgcgcgc gcgaagttgc ctggcgagat ttcgaggcgt tggcgctaaa
    18241 gcggctaacg aaagattgca actcagcaat ctgcgctttc ttcttggcgt tatcggccag
    18301 cagacgttca cgcgcctggg tcgccgccgt catgtactca tcgtagttac ccggataaac
    18361 gcgcagctcg ccgtaatcca gatccgccat gtgggtacag accatgttaa ggaagtgacg
    18421 gtcgtgcgag atgatgatca tggtgctgtc acgctcgttc agcacctgtt ccagccagcg
    18481 aatggtgtcg atgtcgaggt tgttggtcgg ttcgtcgagc aggagaatat ccggctcagc
    18541 aaacagcgcc tgcgccagaa gcacacgcag cttccagcca ggagcaactt cactcatcgg
    18601 gccgtagtgc tgttccactg gaattcccac gccaagcaac agttcaccgg cgcgagcttc
    18661 cgcagagtaa ccgtccattt cgccgtattt cacttccaga tcggccactt tatagccgtc
    18721 ttcttcactc atttccggca aagcatagat gcggtcgcgc tcctgcttca cttcccacaa
    18781 ctctttatgc cccatgatca ccgtatccag cacagtgaac tcttcaaagg caaactgatc
    18841 ctgacgcagc ttaccaatgc gctcgttggg atcgagggaa acgttaccca gcgtcggctc
    18901 aaggtcgccg ccgagaatct tcataaaggt ggatttacca ctaccgttcg cgccaatcag
    18961 gccgtaacgg ttgccgccgc cgaatttgac ggaaatgttt tcaaacaacg gcttactgcc
    19021 gaactgcatg gtgacgttac tggaaactaa cacaggcgta tcctgaaaag agatatgaca
    19081 aaccgcgcat tatgccataa gtctgaaaga agatcctgct cctcgcttaa ggtctacact
    19141 caagccaaag gcaaaaaatg tgatttcgta cacatctgat ttcactgtga gctggaatga
    19201 acttataatg cgcttccaat actctaatat tctcaaccca atggcctgcc aggcacaaaa
    19261 tctcgcttaa catgaatatg aaattgaaaa cattattcgc agcggccttc gctgttgtcg
    19321 gcttttgcag taccgcctct gcggtaactt atcctctgcc aaccgacggg agtcgcctgg
    19381 ttggtcagaa tcaggtgatc accattcctg aaggtaacac tcagccgctg gagtattttg
    19441 ccgcggagta ccagatgggg ctttccaata tgatggaagc gaacccgggt gtggatacct
    19501 tcctgccgaa aggcggtact gtactgaaca ttccgcagca gctgatcctg ccggataccg
    19561 ttcatgaagg catcgtcatt aacagtgcag agatgcgtct gtattactat ccgaaaggga
    19621 ccaacaccgt tatcgtgctg ccgatcggca ttggtcagtt aggcaaagat acgcctatca
    19681 actggaccac caaagttgag cataagaaag caggcccgac ctggacgccg accgccaaaa
    19741 tgcacgcaga gtaccgcgct gcgggcgaac cgctaccggc tgtcgttccg gcaggtccgg
    19801 ataacccgat gggtctgtat gcactctaca tcggtcgcct gtatgctatc catggcacca
    19861 acgccaactt cggtatcggc ctgcgtgtaa gtcatggttg tgtgcgtctg cgtaacgaag
    19921 acatcaaatt cctgttcgag aaagtaccgg tcggtacccg cgtacagttt attgatgagc
    19981 cggtaaaagc gaccaccgag ccagacggca gccgttatat tgaagtccat aacccgctgt
    20041 ctaccaccga agcccagttt gaaggtcagg aaatcgtgcc aattaccctg acgaagagcg
    20101 tgcagacagt gaccggtcag ccagatgttg accaggttgt tcttgatgaa gcgatcaaaa
    20161 atcgttccgg gatgccggtt cgcctgaatt aatcgtcaaa acgtaaaaca aaggcggact
    20221 cataatccgc cttttttatt tgccagacct taccaggccg ggatcataac taacaaaacg
    20281 tatcccacca gcgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
    20341 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc tggtaataaa
    20401 ccaaagcccc ccacgtttac cgcccatgcc agtaataaag acggaggtac ataattgagc
    20461 agcaaaatgg tactcggcac gttactgatc acctgcgata aaccgactgc cgttaaccat
    20521 aacccgggtt cagataaatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
    20581 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcaacgt ccagtccaca
    20641 ctcaacacca cgcgacgcgc cagcagcaca aagcccgccg ccacaatcac cagtccccac
    20701 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaattccac
    20761 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
    20821 gggaaacaac accagcacaa cagcaggagt gtcagcatca ttgcgccagc catcggtgcc
    20881 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
    20941 gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
    21001 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
    21061 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
    21121 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
    21181 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
    21241 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
    21301 ataccaacaa gaattaataa ctgaaaaaac gatcgccttg cagcgtgcgt aaaaaaggca
    21361 ggctcatttg gcaccgtgtt tctgggtaaa caaacgaaaa gcgtcgagcg tctcttcact
    21421 aacatggtgc tccatgcctt ccgcgtcgcg acgggcgatt tccggactga cgcccaacac
    21481 caacaagaaa ttttcgacta tctgatgtcg ctcgcggctt tcctgcgcca gcttctctcc
    21541 ttctgccgtt aaaaacacgc cacgccaggg gatcatttca atcagcccca tggttgccag
    21601 ccgcttaagc attttagcca ccgtcggttg cgaaactccc agacgagcag ccatgtccac
    21661 ctgacgagct tcccccactt ccctgatcaa gtcagaaatc agctcaacgt aatcatcaat
    21721 aagctcgcgc cgatgcgcct ctctgacctg gcggaaccct tcaacgtgct cttccacgtt
    21781 cactaactgc gtcacttttt ttgctattgg cgtacctgcg cgacgactca ttgtgcttcc
    21841 tcattatggt gacgcataca tagcgttctc atttcgtgcg cacattgtaa accagagttg
    21901 cgaaggtaca aaaaattaac gttttagcaa tagctatata atatagcctg tgctatatct
    21961 gtatgtaatg caatcatccc tcaaggatcg acgggattag caagtcagga ggtcttatga
    22021 atgagttcaa gaggtgtatg cgcgtgttta gtcattctcc ctttaaagta cggttaatgc
    22081 tgctctctat gttgtgcgat atggtcaaca acaaaccgca gcaagataaa ccttcagata
    22141 aatagcggcg tcgcggtacg ccgcttcact cctgctttta tgcaggcata acgcgttttg
    22201 gtctgaaaaa aaccactttt tgtcggattt gcaatcccct tcgcaaaaga tttgttcgtc
    22261 agtagttgac ctgaacggcg gctcgctcta tcttcttgca gccctgcgta tattgcggct
    22321 cgcggatgcg gacccctttc ccctcttcac gcactcttgc aggtattgac ccttgacgcc
    22381 agggtaagca catggcgttt gttacgatag tggcatatga atttaaccct caaagaatcg
    22441 cttgttaccc gtagccgggt atttagcccg tggactgcgt tctacttttt acagtcgcta
    22501 ttaattaacc tcggcttagg ttaccccttc agtttgctct acaccgctgc gtttacggct
    22561 attttgcttt tgctatggcg aacattgcct cgcgtacaaa aagttctggt cggtgtcagt
    22621 tcgctggtgg cggcttgtta tttccctttt gctcaggcct acggcgcgcc taatttcaat
    22681 acattgctgg cattgcactc caccaatatg gaagagtcga ccgaaatcct gacgattttt
    22741 ccgtggtaca gctacctggt cggcttattt atttttgcgc tcggcgtaat agcaatcagg
    22801 cgaaaaaaag agaatgaaaa agcgcgctgg aataccttcg acagcctgtg tctggtattc
    22861 agtgtggcga catttttcgt tgctcccgtg caaaacctgg cctggggtgg cgtatttaaa
    22921 ctgaaagata ctggctatcc ggtatttcgt tttgctaagg atgtcatcgt caataataac
    22981 gaggtgattg aagagcaaga acggatggca aaactttccg gaatgaaaga tacctggacg
    23041 gtcactgccg ttaagccgaa gtatcagacc tatgtggtag tgatcggtga aagcgcgcgt
    23101 cgcgatgccc tcggtgcctt tggcggtcac tgggacaata ccccgtttgc cagcagcgtt
    23161 aacggtttga tatttgctga ctacattgct gccagtggct ccacgcagaa atcgcttggc
    23221 ttaacgctca atcgcgtagt cgatggcaaa ccacagtttc aggataactt tgtcaccctg
    23281 gcaaatcgcg cgggcttcca gacctggtgg ttttccaacc agggccaaat cggcgaatac
    23341 gatactgcta tcgccagcat cgccaaacgt gcagatgaag tgtacttcct gaaagaaggt
    23401 aattttgaag cagataaaaa cacgaaagac gaagcgttac tggatatgac cgctcaagtg
    23461 ctggcgcaag agcactcgca accgcagctg attgttctgc atctgatggg ctcgcatccg
    23521 caggcctgcg acaggacaca aggcaaatac gaaacctttg tgcaatcgaa agaaacgtcg
    23581 tgctatctct ataccatgac gcaaacggac gatttactgc gcaagctgta cgatcagtta
    23641 cgcaacagcg gcagcagctt ctctctggtt tacttttctg accacggtct ggcctttaaa
    23701 gagcgcggca aagacgtgca ataccttgcc catgatgata aatatcagca aaatttccag
    23761 gtgcctttta tggtcatttc cagcggcgat aaagcgcatc gggtgattaa agcccgccgc
    23821 tcagccaatg acttcttagg ctttttctcg cagtggacgg gaattaaagc gaaggaaata
    23881 aatatcaaat acccgtttat atctgagaag aaagccgggc cgatatacat caccaacttc
    23941 cagttacaga aggtggatta caaccatctc ggaaccgata ttttcgaccc gaaaccttaa
    24001 aaacaaaaat ccgccccgag aggcggattt ttatatcacc aaagtgatta gaagcggtaa
    24061 ccaacaccgg caatccaggt gcctacgtca acgctacgaa tacggctctg ctcgtaagag
    24121 aagtccagag caacgttttc catcgggttg aactgcagac cagcaccgta ggagaaaccg
    24181 tagtcgctgg tgtcgtgttt gtaggtcggg tattcagtgg tctggaattt accataaccc
    24241 acacccacta caccgtagat gcttgcccag tcgttaatgc ggtaagccgg accagcagtg
    24301 atgccgtagt actggttttt gttgtagtca ccagagcttg cagtacggct tttctcggtg
    24361 taagtgaaag aaccgatcac acccagcggg ctgttgtctt cttcatagcg gtatttcagg
    24421 ttgaaaccgc ccattttgtt catttggccc tgagcgtcgc tctgtgcgta accgccagtt
    24481 acggtagaag tcgcagctac ggaagtacct gcggtgaaag ccagaactgc ggccagtgct
    24541 gaaagacatg caattttttt cataaccacc tcaaatgtga ttcaaataag tcctaagttt
    24601 taaatatatc aaaaattaat gggaaactc
//
LOCUS       DAZXUK010000033        24199 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZXUK010000033 DAZXUK010000000
VERSION     DAZXUK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24199
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.033"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            27..1109
                     /locus_tag="ACH3L8_001097"
     CDS             27..1109
                     /locus_tag="ACH3L8_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFT4084307.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(1598..2396)
                     /locus_tag="ACH3L8_001098"
                     /pseudo
     CDS             complement(1598..2396)
                     /locus_tag="ACH3L8_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(2406..2957)
                     /gene="ybcL"
                     /locus_tag="ACH3L8_001099"
     CDS             complement(2406..2957)
                     /gene="ybcL"
                     /locus_tag="ACH3L8_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFT4084308.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(3126..3458)
                     /gene="emrE"
                     /locus_tag="ACH3L8_001100"
     CDS             complement(3126..3458)
                     /gene="emrE"
                     /locus_tag="ACH3L8_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFT4084309.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(3803..4117)
                     /gene="fliE"
                     /locus_tag="ACH3L8_001101"
     CDS             complement(3803..4117)
                     /gene="fliE"
                     /locus_tag="ACH3L8_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFT4084310.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            4331..5990
                     /gene="fliF"
                     /locus_tag="ACH3L8_001102"
                     /pseudo
     CDS             4331..5990
                     /gene="fliF"
                     /locus_tag="ACH3L8_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            5983..6978
                     /gene="fliG"
                     /locus_tag="ACH3L8_001103"
     CDS             5983..6978
                     /gene="fliG"
                     /locus_tag="ACH3L8_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFT4084311.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            6971..7657
                     /gene="fliH"
                     /locus_tag="ACH3L8_001104"
     CDS             6971..7657
                     /gene="fliH"
                     /locus_tag="ACH3L8_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFT4084312.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            7657..9030
                     /gene="fliI"
                     /locus_tag="ACH3L8_001105"
     CDS             7657..9030
                     /gene="fliI"
                     /locus_tag="ACH3L8_001105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFT4084313.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            9052..9393
                     /gene="fliJ"
                     /locus_tag="ACH3L8_001106"
                     /pseudo
     CDS             9052..9393
                     /gene="fliJ"
                     /locus_tag="ACH3L8_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
     gene            9390..10517
                     /locus_tag="ACH3L8_001107"
     CDS             9390..10517
                     /locus_tag="ACH3L8_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFT4084314.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            10622..11086
                     /gene="fliL"
                     /locus_tag="ACH3L8_001108"
     CDS             10622..11086
                     /gene="fliL"
                     /locus_tag="ACH3L8_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFT4084315.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11091..12095
                     /gene="fliM"
                     /locus_tag="ACH3L8_001109"
     CDS             11091..12095
                     /gene="fliM"
                     /locus_tag="ACH3L8_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFT4084316.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12092..12505
                     /gene="fliN"
                     /locus_tag="ACH3L8_001110"
     CDS             12092..12505
                     /gene="fliN"
                     /locus_tag="ACH3L8_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFT4084317.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            12508..12873
                     /gene="fliO"
                     /locus_tag="ACH3L8_001111"
     CDS             12508..12873
                     /gene="fliO"
                     /locus_tag="ACH3L8_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFT4084318.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            12873..13610
                     /gene="fliP"
                     /locus_tag="ACH3L8_001112"
     CDS             12873..13610
                     /gene="fliP"
                     /locus_tag="ACH3L8_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFT4084319.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            13620..13889
                     /gene="fliQ"
                     /locus_tag="ACH3L8_001113"
     CDS             13620..13889
                     /gene="fliQ"
                     /locus_tag="ACH3L8_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFT4084320.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            13898..14683
                     /gene="fliR"
                     /locus_tag="ACH3L8_001114"
     CDS             13898..14683
                     /gene="fliR"
                     /locus_tag="ACH3L8_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFT4084321.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            14968..15591
                     /gene="rcsA"
                     /locus_tag="ACH3L8_001115"
     CDS             14968..15591
                     /gene="rcsA"
                     /locus_tag="ACH3L8_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFT4084322.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(15635..15823)
                     /gene="dsrB"
                     /locus_tag="ACH3L8_001116"
     CDS             complement(15635..15823)
                     /gene="dsrB"
                     /locus_tag="ACH3L8_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFT4084323.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            15986..16213
                     /gene="yodD"
                     /locus_tag="ACH3L8_001117"
     CDS             15986..16213
                     /gene="yodD"
                     /locus_tag="ACH3L8_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFT4084324.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            16511..17326
                     /locus_tag="ACH3L8_001118"
                     /pseudo
     CDS             16511..17326
                     /locus_tag="ACH3L8_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(17323..19017)
                     /gene="dgcQ"
                     /locus_tag="ACH3L8_001119"
                     /pseudo
     CDS             complement(17323..19017)
                     /gene="dgcQ"
                     /locus_tag="ACH3L8_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(19188..19370)
                     /locus_tag="ACH3L8_001120"
     CDS             complement(19188..19370)
                     /locus_tag="ACH3L8_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFT4084325.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(19449..20366)
                     /locus_tag="ACH3L8_001121"
     CDS             complement(19449..20366)
                     /locus_tag="ACH3L8_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFT4084326.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            20539..21459
                     /gene="yedA"
                     /locus_tag="ACH3L8_001122"
     CDS             20539..21459
                     /gene="yedA"
                     /locus_tag="ACH3L8_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFT4084327.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(21448..21918)
                     /gene="vsr"
                     /locus_tag="ACH3L8_001123"
     CDS             complement(21448..21918)
                     /gene="vsr"
                     /locus_tag="ACH3L8_001123"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFT4084328.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(21899..23317)
                     /gene="dcm"
                     /locus_tag="ACH3L8_001124"
     CDS             complement(21899..23317)
                     /gene="dcm"
                     /locus_tag="ACH3L8_001124"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFT4084329.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(23384..23885)
                     /locus_tag="ACH3L8_001125"
                     /pseudo
     CDS             complement(23384..23885)
                     /locus_tag="ACH3L8_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(23964..>24199)
                     /gene="drpB"
                     /locus_tag="ACH3L8_001126"
     CDS             complement(23964..>24199)
                     /gene="drpB"
                     /locus_tag="ACH3L8_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFT4084330.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6043 a         6247 c         6030 g         5879 t
ORIGIN      
        1 taatagttca aataaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
       61 ctgcatcagt actgatggcg atgtctgctc aggcagcaga gatttacaat aaagacagta
      121 acaagctgga tctgtacgga aaagttaatg ctaagcacta cttctcttct aacgatgcag
      181 atgatggcga cacaacttat gttcgtctgg gcttcaaagg cgaaactcaa atcaacgatc
      241 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgtgct gaatctcaag
      301 gttcttccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
      361 ccatcgatta tggccgtaac tacggtgtag catacgatat cggtgcatgg accgacgttc
      421 tgccagaatt cggtggcgat acctggaccc aaacagatgt gttcatgact ggtcgcacca
      481 caggtgttgc aacctatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      541 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaac ggtgatggtt
      601 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
      661 ctgaccgcac taatgatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
      721 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
      781 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
      841 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
      901 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg attgaatata
      961 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1021 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1081 ttgctgtagg attggtttac cagttctaat ctgattactc aaagatatgt tgtgggaggc
     1141 tttgcctccc caacatataa gtggcgctct caagccactt tccttagaag cactatcttg
     1201 cttctgacta tataaattac cctttatttt ctgggacgtt tcaacgcccc atttttaata
     1261 atactcagtg aacaattaac gtatcaatta gatttaataa caacgatatc cgtacctaac
     1321 ctgatatcta atgtcataag catcccttta attatcccct caacctcttg taaatttccc
     1381 actcttgagc tccacatagc ccaaacgccg gatgcaaaaa aaatcatacg tgcatctctg
     1441 aaattgtgat ggaggaagtg agtatcaatt atatgtgctt ataaacgttc ccgattggca
     1501 cagaagtcac cggattgttc aggctccaat aggttgagta tgtcgtattt attttccaaa
     1561 ataaaaatcg atgctgttgc tccttaataa aatactttta ctctctggac gcttttctta
     1621 gctctcttgg tgttatatta aaacgattat ggaacctttc agtaaaacga gaaggacact
     1681 tgtaaccatt ttccctggca atctcgctga tgggttttac tgtcgtttgt atagcaggac
     1741 aacgcattat ttaacctcac gtcgtccaat atactttgaa aacttattcc ctcgcttgcc
     1801 agacggcgat gcaatgtgga aacagaaatg tagagatatc tcgctacctt gtttgctgtc
     1861 cattttgttc taggttcgga tagcattagg ttataacaac gacttattaa tgattgttta
     1921 ctatctgata aaagtaaaca attaacatga tgtgctccta atgaaagtag aacgcccatt
     1981 gctaagtgtt cctgaatttg cgttgagaag cctcgggaaa tagatgtttt tagttgctcc
     2041 caacaatata ttaactcggg gttctgaggt aagcagaaac ctgtcgtgtt acgaatttga
     2101 tcagttatcg cataaaattt ttgaaacttt tcaattagat caatgggata ataaagcatt
     2161 tctgcaagat aaagacctgc ttctggataa ttcacaatat aaaattcata tccacaggga
     2221 aataatatta tctgattatt atcaacagtt agactatgcg tctcccaatt gataactttt
     2281 tttccctgac ggatacgaca taaagctggc ataagaggct taaccctgtg aatctcatga
     2341 tgtttatgca tccgtatttc ttcaatcttt aggttagact tatctcttgc cagcatactc
     2401 ttaccctatt ttatctcata aactggtgtt atctcagcgg ttgcaatttt attggcatta
     2461 agcatataac caactaacgc tccgctggaa ttagaatcca caggaatctt atcagttttt
     2521 agagcccata ctttaaactg gtaatgatgg ggtttatctc ctttgggagg acatgctcca
     2581 ccaaatcctg cataaccaaa atcatttcgg ccttgaacag caccagtcgg aagttttgta
     2641 ccatcacgtc ttcctgcatc agtgggcaaa tatgttacag ttgctggaat attagcaaca
     2701 gtccagtgcc accatccact gcctgtagga gcatctggat catacacagt tacggcaaag
     2761 cttttggtgc cttcaggagc accagaccag gttaatgagg gcgatgtatt accaccttca
     2821 cacccaaatc cagaaaatac atgagacgtt gtaagttgct ctcctgtttt tatttcatta
     2881 ctagtgacct gaaatgctgc ggcctgcgca gaaaatgtta tgaatgccaa tacacttgaa
     2941 acgataagtt ttttcataaa aacctcattg ttgtgaccta tcgttatttt atttgatgtg
     3001 ccttgatctc attatgcata aaggcgcaat gatcgcgcaa atgcaatcat aattataaag
     3061 gtgccccgaa cattagccat aatatgcaca aatcagattc ctgttatctc aaggagaatt
     3121 attttttaat gcggtgcact tcgtgacaac aagttaatga ctaacacacc ggcacaaatc
     3181 aacatcatgc ctatgatagc tggcaggtcc agtcgttgac caaaaaatcc ccaggaaagc
     3241 aaactaatca gaacgatacc gactcctgac cagatagcat aagcaatccc tgtaggtata
     3301 taagccagcg tctgagctaa taaccagaat gatgcacaat agcaaataat tgtgccaaca
     3361 gagggccata accgtgtaaa cccctctgaa aacttcatta aggttgtacc aatgacctct
     3421 gcaagtattg cgccaccaag ataaatataa gggttcatag catattcttt cctgttcata
     3481 ctggagagaa ttgtactaca gtttgaacac aactcaccgt tttcatcctg gtgtatgtgt
     3541 atccaagggg ctctggcgtg gcaagagata aaggcaggaa caataatgat cagaaggaac
     3601 cccaggctaa cgaatgagtt cactcttcct gggagcaaag cgaagacaaa acatgttatc
     3661 gcgtttacgg atcgtggatg gcagaaaaca accaggatca ggtatccatt ttaaaccaga
     3721 aattgagtga gtttgcccca tccatgcccc acgctgtggg atcggatata attaaacaag
     3781 catagtcaac aggttacaca acctacacct gcatggacat cacttcctga tacgccgcca
     3841 ccagcttatt acgcacctga atccccattt gcatagaaac tgaggctttt tgcatatcgg
     3901 tcatcacatc gtttagcgcc acgccgggtt caccgagggt gaatttttct gcctgcgtgc
     3961 gggcgactgt ttgtgtatcg cttatgcgat cgagcgcggc gtgcagctgc ccggcaaaac
     4021 taatggtcgg ttgcggcagt gattcctgcg cacgcgcact catcgccgta gcctgtaact
     4081 ggctgataac cccttcaatc ccctgtatcg ctgacatttt cgtctcccgg ataatttctg
     4141 gtaacaaagc ctaccagtaa gtcaataaga caaaggcgct aaatagcaac aaaaaaactg
     4201 gtttattggc ggatagaaaa aacgaaagca caaataatgg gagcgtcaat ttttcgagtg
     4261 tgctgacccg agagtgagtc ttgttccact ttgccaataa cgccgtccat aatcagccac
     4321 gaagtgcgcg atgaatgcga ctgcagccca gacaaaatct cttgagtggc ttaatcgcct
     4381 gcgtgcgaat ccgaaaattc cattgattgt taccggttcc gcggcagtgg cggtcatggt
     4441 cgcactgatc ctgtgggcga aagcccccga ctaccgcaca ttattcagca atctttccga
     4501 tcaggatggt ggcgcaattg tcagccaact gacgcaaatg aatattcctt accgcttcag
     4561 cgaagccagc ggcgctattg aggttccggc agataaagtt cacgaactgc gcctggcgca
     4621 acaaggtttg cccaaaggcg gcgcggtcgg tttcgaactg ctcgatcagg aaaagtttgg
     4681 tatcagccag ttcagcgaac aggtgaatta tcagcgggcg ctggaaggcg agctttctcg
     4741 taccatcgaa actatcggcc cggtaaaagg ggcgcgcgta catctggcaa tgccgaaacc
     4801 gtctttattc gtccgtgaac aaaaatcccc ttctgcatcg gtgacggtaa atctgttacc
     4861 cggccgcgca ctcgatgaag ggcaaattag cgctattgta catctggttt ccagcgcggt
     4921 tgctggtctg ccgcctggaa acgtcacgct ggtggatcag ggcgggcatc tgttaaccca
     4981 gtccaatacc agcgggcgcg atcttaatga cgctcagttg aaatatgcca gcgatgtcga
     5041 aggccgtatt cagcagcgta ttgaagcgat cctgtcacct attgttggta acggtaatat
     5101 tcacgcctag gtcacggcgc aactggactt cgccagtaaa gaacaaacgg aagaacagta
     5161 tcgccctaac ggtgatgaat ctcatgcggc gcttcgttca cgccagctta atgagagcga
     5221 gcaaagcggt tccggttatc cgggcggcgt accgggggcg ttgtcgaatc aaccggcacc
     5281 tgcgaataac gcgccaatca gcacgcctcc gacaaatcaa aataaccgcc agcagcaggc
     5341 gagcaccacc agcaatagtg ggccgcgtag cacacagcgg aatgaaacca gtaactacga
     5401 agtcgatcgc accattcgtc ataccaaaat gaacgtgggc gatgtgcaac gtctgtcagt
     5461 cgcggtagtg gtgaattaca aaaccttgcc agacggtaaa ccattgcctc tcagcaacga
     5521 acagatgaag caaattgaag ctctgacccg cgaggcgatg ggcttttctg aaaaacgcgg
     5581 tgattcgctc aatgttgtta actcgccgtt caatagcagt gacgaaagcg gcggagcact
     5641 gccgttttgg caacagcaag tgtttatcga tcagttgctg gctgccggtc gctggttgct
     5701 ggttgctggt actgctggtg gcgtggttgc tgtggcggaa agcggtacgc ccgcagctaa
     5761 cacgtcgcgc cgaggcggtg aagactgtgc agcaacaggc gcaggcccgc gaggaagtgg
     5821 aagatgcggt ggaagtccgt ctgagcaaag acgaacaact ccaacaacgg cgcgctaacc
     5881 aacgactggg ggcagaagtc atgagccagc gtatccgtga aatgtctgat aacgatccgc
     5941 gcgtggtggc gctggtcatt cgccagtgga taaataacga tcatgagtaa cctgacaggc
     6001 accgataaaa gcgtcatcct gctgatgacc attggcgaag accgggcggc agaggtgttc
     6061 aagcacctct cccagcgcga agtgcaaacc ctgagcgctg caatggcgaa cgtcacgcag
     6121 atctccaaca agcagctaac cgatgtgctg gcggagtttg agcaagaagc tgaacagttt
     6181 gctgcactga atatcaacgc caacgattat ctgcgttcgg tattggtcaa agctctgggt
     6241 gaagaacgtg ccgccagcct gctggaagat attctcgaaa cccgcgatac cgccagcggt
     6301 attgaaacgc tcaactttat ggagccgcag agcgccgccg atctgattcg cgatgagcat
     6361 ccgcaaatta tcgccaccat tctggtgcat ctgaggcgcg cccaggccgc cgatattctg
     6421 gcgttgttcg atgaacgtct gcgccacgac gtgatgttgc gtatcgccac ctttggcggc
     6481 gtgcagccag ccgcgctggc cgaactgacc gaagtactga atggcttgct cgacggtcag
     6541 aatctcaagc gcagcaaaat gggcggcgtg agaacggcag ccgaaattat caacctgatg
     6601 aaaacgcagc aggaagaagc cgttattacc gccgtgcgtg aattcgacgg cgagctggcg
     6661 cagaaaatca tcgacgagat gttcctgttc gagaatctgg tggatgtcga tgatcgcagc
     6721 attcagcgtc tgttgcagga agtggattcc gaatcgctgc tgatcgcgct gaaaggggcc
     6781 gagcagccac tgcgcgagaa attcctgcgc aatatgtcgc agcgtgccgc cgatattctg
     6841 cgcgacgatc tcgccaagcg tggtccggtg cgtctgtcgc aggtcgaaaa cgaacagaaa
     6901 gcgattctgc tgattgtgcg ccgtcttgcc gaaactggcg agatggtgat tggcagcggc
     6961 gaggatacct atgtctgata atctgccgtg gaaaacctgg acgccggacg atctcgcgcc
     7021 accaccggca gagtttgtgc caatggtcga gtcggaagaa accattattg aagaggccga
     7081 acccagcctt gagcagcaac tggcgcaact gcaaatgcag gcccacgagc agggttatca
     7141 ggcggggatt gccgaaggtc gccagcaagg tcacgaacag ggctatcagg aaggactggc
     7201 ccaggggctg gaacaaggtc tggcagaggc gaaggctcaa caagcgccaa ttcatgcccg
     7261 gatgcagcaa ctggtcagcg aatttcaaac tacccttgat gcgcttgata gtgtgatagc
     7321 ttcgcgcctg atgcagatgg cgctggaggc agcacgtcag gtgatcggtc agacaccaac
     7381 ggtggataac tcggcactga tcaaacagat ccaacagttg ttgcagcaag aaccgttatt
     7441 cagcgggaaa ccacagctgc gcgtacaccc ggatgatctg caacgtgtgg atgatatgct
     7501 cggcgcaact ttaagtttgc atggctggcg cttgcggggc gatcccaccc tccaccctgg
     7561 cggatgtaaa gtctccgccg atgaaggcga tctcgacgcc agtgtcgcca ctcgctggca
     7621 agaactctgc cgtctggcag caccaggagt ggtgtaatga ccacgcgcct gactcgctgg
     7681 ctaaccacgc tggataactt tgaagccaaa atggcgcagt tgcctgcggt acgtcgctac
     7741 gggcgattaa cccgcgctac cgggctggtg ctggaagcca ccggattaca attgccgctc
     7801 ggcgcaacct gtgtcattga gcgccagaac ggcagcgaaa cgcacgaagt agaaagcgaa
     7861 gtcgttggct ttaacggtca acggctgttt ttaatgccgc tggaggaagt ggaaggtgtc
     7921 ctgcccggcg cgcgtgtata tgccaaaaac atttcggcag aagggctgca aagcggcaag
     7981 cagttgccgc tcggtccggc gttattaggt cgtgttctgg acggtagcgg taaaccgctc
     8041 gatggcctgc ccttccccga tacgacggaa accggtgcgc tgattacccc gccatttaac
     8101 ccgttgcaac gtacaccgat tgaacatgtg ctggacaccg gcgtgcgccc aatcaatgcc
     8161 ctgcttaccg tcggacgcgg gcagcgtatg gggctgtttg ccgggtcggg cgttggtaaa
     8221 agtgtgctgc tggggatgat ggcacgctac acccgcgccg atgtcattgt cgtgggtttg
     8281 attggtgaac gtgggcgcga agtaaaagat tttattgaga atatcctcgg tgccgaagga
     8341 cgtgcacgct cagtggtgat tgccgctccg gcggatgttt ctccgctcct gcgaatgcaa
     8401 ggtgccgcct atgccacgcg cattgccgaa gattttcgcg atcgtggtca gcatgtgttg
     8461 ctgattatgg actccctcac ccgctacgcg atggcccagc gtgaaattgc gctggcgatt
     8521 ggcgaaccac ctgccactaa aggttatcca ccgtcggtgt ttgccaaatt accggcactg
     8581 gtcgagcgtg ccggtaatgg cattagcggc ggcggctcga ttaccgcgtt ttataccgtg
     8641 ctcaccgaag gcgatgacca gcaggacccc attgctgact ccgcgcgggc aatcctcgac
     8701 ggtcacattg tgctgtctcg ccgactggcg gaagccgggc actatccggc tatcgatatt
     8761 gaagcgtcga tcagtcgcgc aatgacggcg ttgatcagtg agcaacatta cgcgcgagtg
     8821 cgcaccttca aacagctgtt gtcgagtttt cagcgtaacc gcgatctggt tagcgtcggc
     8881 gcgtatgcca aaggcagcga tccgatgctc gataaagcca tcgccctgtg gccgcagctg
     8941 gagggctatt tgcaacaagg catttttgaa cgcgcggact gggaagcgtc tctccagggg
     9001 ctggagcgta ttttcccgac agtgtcataa cccaggagat aacggcagat ggcggaagaa
     9061 cagctcaaaa tgttgatcga ttatcagaat gaatatcgca ataacctcaa tagcgatatg
     9121 agtgccggga tgaccagcaa ccgctggatc aactatcagc agtttatcca gacgctggaa
     9181 aaagccatta ctcagcatcg ccagcaactt aatcagtgga cgcagaaagt tgacattgcc
     9241 ctgaacagct ggcgagaaaa gaaacaacgt ttgcaggcct ggcagacact gcaggaacgg
     9301 caatccacgg cggcactgct tgcagaaaac cgcctcgatc agaaaaagat ggatgagttc
     9361 gcccagcgcg ccgccatgag gaaacctgaa tgattcgctt agcgcccttg attaccgccg
     9421 acgttgacac caccacattg cctggcggca aagccagcga tgctgcacaa gattttctca
     9481 cgttgttgag cgaagcatta gcaggcgaga caacaactga caaagcggcc ccccagttgc
     9541 tggtagcaac agataagccc acgacaaaag gcgagccgct ggtcagcgat attcttgccg
     9601 atgcgcaaca agcggattta ctgatccctg tggatgaaac acttcctgtc atcaacgacg
     9661 aacaatccac atcaacaccg ttaaccaccg ctcagacgat gacgttggct gcggtggctg
     9721 acaaaaatac gacaaaagac gaaaaagcgg atgatcttaa tgaagacgtc accgcaagtc
     9781 tgagcgccct ttttgcgatg ttgccgggtt ttgacaatac gcccaaagtg actgatgcac
     9841 cgtcaaccgt gttaccggca gagaaaccaa cgctcttcac aaaactgact tctgcgcaac
     9901 tcactacagc acagcctgat gacgcccccg gcacaccagc tcagccatta acaccgctgg
     9961 tagcagaagc ccagagtaaa gcggaagtca tcagcacgcc ttcgccggtg accgctgacg
    10021 ccagcccgct aatcactcca caccagacac agccactgcc caccgtcgcc gcgcctgttt
    10081 tgagtgcacc gctgggttct cacgaatggc aacaatcatt gagccagcat atttcgctgt
    10141 tcacccgcca ggggcaacaa agtgcagagt tgcgtctgca cccacaggat ttaggtgaag
    10201 tgcaaatttc cctcaaagtg gatgataacc aggcgcagat ccagatgatt tcaccgcatc
    10261 agcacgtacg cgccgccctg gaagcagcgc tgccggtact gcgtacgcag ctggccgaaa
    10321 gtggcattca gttagggcaa agcaacatta gtggcgaaag ctttagtggt cagcagcagg
    10381 ccgcttccca gcaacagcaa agccaacgca cagcaaacca tgaacctctg gcgggggaag
    10441 acgacgatac gcttccggtt cccgtctctt tacaagggcg tgtaacaggt aacagcggcg
    10501 ttgatatttt cgcctaacgt cagaggtagc accgtaatcc gcgtcttttc cccgctttgt
    10561 tgcgctcaag acgcaggata attagccgat aagcagtagc gacacaggaa gaccgcaaca
    10621 catgactgat tacgcgataa gcaagaaaag caagcgatcg ctttggatcc cgattctggt
    10681 attcattacc ctcgcggcct gtgccagcgc aggttacagc tactggcatt cgcatcaggt
    10741 tgccgctgac gacaaagcgc agcaacgcgt cgtgccctcc ccggtcttct atgcgctgga
    10801 tacctttacg gtcaatttgg gcgatgcgga tcgcgtgctt tatatcggca taaccctgcg
    10861 actgaaagat gaagcaaccc gctcgcggct gagtgagtat ttgccggaag tccgtagtcg
    10921 cttgctgtta ctgttttcac gtcaggatgc tgccgtactg gcgacagaag aaggcaagaa
    10981 aaacctgatt gccgagatta aaaccacact ttccaccccg cttgttgccg ggcaaccgaa
    11041 acaggatgtc accgacgtgc tgtataccgc ttttattctg cgataacgac atgggcgata
    11101 gtattctttc tcaagctgaa attgatgcgc tgttgaatgg tgacagcgaa gtcaaagacg
    11161 aaccgacagc cagtattagc ggcgaaagtg acattcgtcc gtacgatccg aatacccaac
    11221 gacgggttgt gcgcgaacgt ttgcaggcgc tggaaatcat taatgagcgc tttgcccgcc
    11281 attttcgtat ggggctgttc aacctgctgc gccgtagccc ggacattacc gtcggggcca
    11341 tccgcattca gccgtaccat gaatttgccc gcaacctgcc ggtgccgacc aacctgaacc
    11401 taatccacct gaaaccgcta cgcggcacag ggctggtagt gttctcgccg agtctggtgt
    11461 ttatcgccgt ggataacctg tttggcggcg atggacgctt cccgaccaaa gtggaaggtc
    11521 gcgagtttac ccatactgaa cagcgcgtca tcaaccgtat gttgaagctg gcgctggaag
    11581 gctatagcga cgcctggaaa gcgattaatc cgctggaagt tgagtacgtg cgttcggaaa
    11641 tgcaggtgga atttaccaat atcaccacct cgccgaacga cattgtggtt aacacgccgt
    11701 tccatgtgga gattggcaac ctgaccggcg aatttaatat ctgcctgcca ttcagcatga
    11761 tcgagccgct acgggaattg ttggttaacc cgccgctgga aaactcgcgt aatgaagatc
    11821 agaactggcg cgataacctg gtgcgccagg tgcagcattc acagctggaa ctggtggcca
    11881 actttgccga tatctcgcta cgcctgtcgc agattttaaa actgaaaccc ggcgacgtcc
    11941 tgccgataga aaaacccgat cgcatcatcg cccatgttga cggcgtcccg gttctgacca
    12001 gtcagtacgg tactctcaac ggtcagtatg cgttacggat agaacatttg attaacccga
    12061 ttttaaattc tctgaacgag gaacagccca aatgagtgac atgaataatc cggccgatga
    12121 caacaacggc gcaatggacg atctgtgggc tgaagcgttg agcgagcaaa aatcaaccag
    12181 cgaaaaaagc gctgccgacg cggtgttcca gcaatttggc ggtggtgatg tcagcggaac
    12241 gttgcaggat atcgacctga ttatggatat tccggtcaag ctgaccgtcg agctgggccg
    12301 tacgcggatg accatcaaag agctgttgcg tctgacgcaa gggtcagtcg tggcgctgga
    12361 cggtctggcg ggcgaaccgc tggatattct gatcaacggt tatttaatcg cccagggcga
    12421 agtggtggtt gttgccgata aatatggcgt gcggatcacc gatatcatta ccccgtccga
    12481 gcggatgcgc cgcctgagcc gttagtgatg aataaccatg ctactgtgca atcttccgcg
    12541 ccggtttctg ctgagccact gctgcaggtg agcggcgcac tcatcgccat tattgccctg
    12601 atcctcgctg ctgcctggct ggtaaaacgg ctgggatttg cccctaaacg cactggcgtt
    12661 aacggtctga aaattagcgc cagtgcttcg ctgggcgcgc gtgaacgggt cgtggtggtc
    12721 gatgtggaag atgcacggct ggtgctcggc gttaccgcag ggcaaatcaa tctgctgcat
    12781 aaacttcccc cttcagcacc aacggaagag ataccgcaga ccgattttca gtcggtcatg
    12841 aaaaatttgc ttaagcgtgg cgggagatcc tgatgcgtcg tttgttttct gtcgcaccgg
    12901 tccttctctg gctgattacg cccctcgcct tcgcgcaact gccgggtatc accagccagc
    12961 cgctacctgg cggtggacaa agctggtcgc tcccggtgca gacgctggtg ttcatcacct
    13021 cgttgacgtt cattccagcg attttactga tgatgaccag tttcacccgc atcattattg
    13081 tttttggttt attgcgtaac gcgctgggaa cgccctccgc gccacctaac caggtattgc
    13141 tggggctggc actgtttttg acctttttta ttatgtcgcc agtgatcgac aaaatttatg
    13201 tcgatgcgta ccagccattc agcgaagaga aaatctcaat gcaggaggcg ctggaaaaag
    13261 gggcgcagcc gctgcgtgag tttatgctgc gtcagacccg cgaggcagat ttagggttgt
    13321 ttgccagact ggcgaatacc ggcccgttgc aggggcctga agctgtgccg atgcgcattt
    13381 tgctcccggc ctacgtgacc agcgagttga aaaccgcatt tcagataggc ttcacgattt
    13441 tcatcccttt tttgattatc gacctggtga ttgccagcgt gttgatggca ttggggatga
    13501 tgatggttcc ccccgccacc attgctctgc cctttaaact gatgctgttt gtactggtgg
    13561 atggctggca attgctggtc ggttcgctgg cgcagagctt ttacagctag agaggcaaaa
    13621 tgacacctga atcggtcatg atgatgggaa ctgaagcgat gaaagtcgcg ctggcactgg
    13681 cagccccgct gttgctggta gcgctggtca cgggccttat catcagtatt ttgcaggcag
    13741 ccacgcagat taacgaaatg acgctgtcgt ttattccgaa aatcatcgcc gtatttatcg
    13801 ccatcattat tgccggaccg tggatgctca atctgttgct ggattacgtc cgcaccttgt
    13861 tcactaacct gccgtatatc atcgggtagc ccgtacaatg atgcaggaga caagcgatca
    13921 atggctttcc tggttaagcc tgtacttctg gccgttactg cgcgtgctgg cgctcatttc
    13981 taccgcgcca attctgagcg aacgcagcgt gccgaaacgg gtaaaactgg gtctggcaat
    14041 gatgatcacg ttcgccattg ccccatcatt acctgccaac gatgttcctg ttttttcgtt
    14101 ctttgctctg tggctggccg tacagcagat cctgatcggc attgcgcttg gttttaccat
    14161 gcaatttgcc tttgccgctg tgcgaaccgc tggcgaaatt atcggtctgc aaatggggct
    14221 gtcatttgcg acatttgtcg atcctggcag ccatctcaat atgcccgttt tagcgcgcat
    14281 catggatatg ctggcattac tgctgttcct gacatttaac ggtcatttat ggttgatttc
    14341 actgctggtc gatacctttc acaccctgcc gattggtagc gaaccgttga acagcaatgc
    14401 gtttctggca cccaccaaag cagggagttt gattttcctt aacgggctga tgctggcgtt
    14461 accgctcatt actctgttgc tgaccctgaa tctggcatta ggtttactga atcgtatggc
    14521 cccgcaatta tccatttttg ttattggatt tccattaact ctgactgtcg gcatctcttt
    14581 aatggcggca ttaatgccgt taattgcgcc tttttgcgaa catttattca gtgaaatttt
    14641 taatttgctg gctgatatta ttagtgaatt accattaata taattccgta acgtttatca
    14701 tgctacccta aggattatcc gaaaaataat atctgcgaac atcttccagg atactcctgt
    14761 agcgaaatat ttgttttaag ctcactcaca tatcgcaaca tttactttaa gacaattcca
    14821 ggcaaattat acaacacttt acgggatagt aagtccgcct gaaaaattgc gagagtggcg
    14881 cattaggtga cccatgttgt tccgtttagt catgatgaaa tattcaggta aggggaatta
    14941 tcgttacgca ttgagtgagg gtatgccatg tcaacgatta ttatggattt atgtagttac
    15001 acccgactag gtttaaccgg gtatctgttg agtagagggg ttaaaaaaag agaaatcaac
    15061 gacattgaaa ccgttgatga ccttgccata gcttgtgatt cacagcgtcc ttcagtggtg
    15121 tttattaatg aggactgttt catccacgat gcttctaaca gtcagcgcat caagcacatc
    15181 attaatcaac atcccaatac gttatttatc gtttttatgg caattgccaa tgttcatttt
    15241 gatgaatatc tattggtcag aaaaaattta ttgatcagtt ctaaatcgat taaaccggaa
    15301 tctctcgacg atatccttgg cgatattctg aaaaaagaga caacgataac ctcgttttta
    15361 aatatgccga cgttatcatt gagccgaacc gaatcgagta tgttgcgaat gtggatggca
    15421 ggtcagggaa ccattcaaat ctccgaccaa atgaatatca aagccaagac cgtttcatcg
    15481 cataaaggta atattaaacg taagatcaaa acgcataata aacaggttat ctaccatgtc
    15541 gtccgactga cggataatgt gactaatggt atttttgtca acatgcgcta acacattctg
    15601 actggtggtt tcccaccagt caggctgaat aagattactc tgctttctca acaaagatac
    15661 cgtcctgatg ccctgcttca ttaaagaacc agatgccgag cgggtagtct tccagcgcaa
    15721 ccaggtacat tgtgccttca ctaaactcct caactgccag taccactcca ggacgacgcg
    15781 gaccgccatc cgttttgact gttacccgat cattcacctt catcgttttc ctcctgtggc
    15841 tttgtgccag tgtagaacaa tttcgttttt ctggcagcgc caagcgcgcg cgagtgctga
    15901 ttttctcgac ggtctatact taagagatgc caggcggact taacgactgg cggcaacaac
    15961 agagtaacgg ttgcgaggaa agatgatgaa aaccgcaaaa gtgtacagcg ataccgcaaa
    16021 acgtgaggtc agcgtcgatg tcgatgccct gctggcggcg atcaatgaaa ttagcgaaag
    16081 cgaagttcat cgcagccaga acgattctga acacgttagc gtcgatggac gtgaatatca
    16141 tacatggcgt gaattggcgg atgccttcga actggatatt catgacttca gcgtctctga
    16201 agtgaatcgt tgaatgcaca ataaaaaaat cccgaccctg agggtgtcgg gatgaaactt
    16261 gcttaagcaa gaagcactta aaaaattcgt tacaccagga aatctgatgt gttcatcacc
    16321 ttatctgcaa tttttttcgc tgacaagaaa atattcgcgc tatgaatgat taacgctact
    16381 tatgaataga aatgtgacgc gcgtcacccc ttggtggctc tggtgtcaac gggatcgctc
    16441 ccgaaaaaat catctttgat tatttgtgct gttttgagaa tcatcctggt aaggggagta
    16501 ctgcgcgacc atgttttcaa ttcaacaacc actactggtt tttagcgatc ttgatggcac
    16561 cctgctggac agtcatagtt atgactggca accggcagcc ccctggctca gccgtttaca
    16621 cgaagccaat attcccgtca ttctctgtag cagtaaaact ttagcggaaa tgctgtactt
    16681 gcaaaaaatg ttggggctac aaggcttacc gctgattgca gagaatggcg cagtgatcca
    16741 gcttgctgaa caatggcagg atatagacgg ttttccacgc atcatctcag gtattagcca
    16801 tggcgaaatc tgccaggttt taaatacgct acgcgagaaa gaacatttta aattcacgac
    16861 ttttgatgat gtcgacgatg caaccattgc cgaatggacg ggattaagcc gtagctaggc
    16921 ggcgctgacg cagctgcatg aggcgtcggt aacgctaatc tggcgcgaca gtgacgagcg
    16981 tatggcacaa tttatcgctc gtctgaacga actgggcttg cagtttatgc agggtgcgcg
    17041 cttctggcac gtactggatg cctctgccgg aaaagatcag gctgccaact ggattatcgc
    17101 gacctatcaa caattgtcag gcagacgccc aaccacactt ggcctgggcg atgggccaaa
    17161 cgatgcgcct ttactggagg taatggatta cgcggtgatt gtgaaagggc taaaccgtga
    17221 aggggtacat ctgcatgatg aggatccggc ccgcgtctgg cgaacgcagc gtgaaggacc
    17281 ggaaggctgg cgtgaagggc tggaccattt tttctccgcc cgttaagcgt tatcgctcgc
    17341 gaatacccga ttgcgcccgg cctgttttgc gaggtaaagc cgacggtcgg ccaatgactg
    17401 gagttgttca aaatcataat caccggtttc ctcgctgcta cttaccccca gcgaggcact
    17461 gatgcgtatc gttgtactct tggcgatcag catctctttt tcatttaact tcaggcgaat
    17521 acgttctgcg acctccgcag cctgcgtcag attcgcgcct ggcagaatca cacaaaactc
    17581 ctcaccaccg acccgcccgg caacgtcctg cgcacgcaag gaactgctaa ttaatccggc
    17641 agcatgagaa agaacacggt cgcccgcctg atgaccaaag cggtcattaa tcgctttaaa
    17701 atggtcaaga tcgacctgaa tgacagaaaa aggatgttgg tgcgtctgac acaatttagc
    17761 gagcggacgg gctttttcga acagtgcgcc acggttatat aaacgcgtta aggtgtcgtg
    17821 ccacgcctgc cactgcaacg agctttgcag aacatacatg ttgctgacca tccggcgaat
    17881 cacataccag gagaggagta acatggtggt aaagagcgcc cacagcaggg ttaatgcaat
    17941 gctgatactg ccgaaatcgc cgcgcacgcc ttcgcttagc gtatggacgc gcgccagcac
    18001 accgtcgaaa tggtccagac gttcccagct aacatagcga ctgtccatac gaatgccgcc
    18061 ccgcgtgtca tgttccattg cctgcgccag caaggccagt tcacgaggat caaaaatatt
    18121 ccctgttgga tggtcaggat tggaagaggt caaaaatctc agcttactgt catagagctg
    18181 atactcacca tcgaggtttt tatcgatggc gtttcttaaa aattgctgca tagtacgcac
    18241 gggaatactc atccccagca cgccatacca gtagttatta ctgtctaccg gaacactgac
    18301 ggtaacctgc ggttcagtat tgctggcgtg ttccggttgc gaagtgaacc agcgtaccgc
    18361 gcggtgacga ttttctcgtt gcgaatggcc gataaaccag ggttgggtga catagccgta
    18421 ataacgcgtt ggtacattgc gcgtaaacaa ggtcggctgc gtcgaaacgt aaaatccagc
    18481 acgcgagaca tacatcgcct gttcaaccat cgacgaggag ttgtgcgcca gtcgcagcaa
    18541 gtaaccaact tccagtgcag cggtaatttc attgtcgagg ctttcatttt cgcgagacag
    18601 gaaattcccc tcgctgacta atgcatccga tacgccattg actggcaggg tgcgccgtcg
    18661 gttgagttcg atttgccagg cgtgctcgtc gcgatgctgt tcgaactcgg ttacggcatt
    18721 acgcagagag gtgaaatcca gtggcgccac gagagcttcg cgcatgccat tacgcaaaaa
    18781 gatcagtttg tcgacgttat actgcaaatg cttatcgagc gcgttggcta cgttttccag
    18841 atgattacgc tagctggaga tataggcatc ttccagcacc accacttcac gccaggtgag
    18901 caaggtggaa aaaagcaata ccacgataaa gcagagatta acgacatgac cgggccccag
    18961 gcggcgtgcg agttttttca accagctctg gttttccatt tttgtctcgt gctgcaccct
    19021 gacgactccc aacctgcttt tttatgattc tggcataacc cggcaatagc gtaccgcaaa
    19081 acagcgataa ttttataatg gtggcatgat aagacgcgac tatcattcat tgcaggcatt
    19141 gggacaccag gccggatgtg atccggcctg gtgttaatga taccctttca aacttcttcc
    19201 gcagaacgac tcccctcccc cggaacaaga tcggtttcat gaaaagcttc ccgcttgacc
    19261 ccgtaaccgt cataccaacg acactcaacc ataccgctgg agtatccagt gacaatcatc
    19321 cgcgggccgc cctctttaac cgtaacttcc tcactaacca taaagctcat actcgcctcc
    19381 ttttttcgag tgaaacttgt tcaccttagt tgaagatggc gaattttgca taatagccag
    19441 tgcgagtatt aatgtgcctg accgcgcatt ttcgctactg ctttcactcc cagcaccacg
    19501 atgccgccga tgataaatcc aagaatcaga tttaaaacag tcggtaatat catcgccacc
    19561 actgcacttt gctgcccggc gatatgttca atagcgtgat gcagcggcgc aataccatgt
    19621 accacaatcc cgccgccgac gaggaacatc gccagcgtgc cgacaatcga taatgctttc
    19681 atcagccaag gcgcgataat caataaccct ttacctaatg cctgcatcag cgcgctagat
    19741 ttttccgcca gccaataccc taggtcatca atcttaacga taacccctac cagaccgtag
    19801 acgcccacgg tcactaccag cgcgatgcct gaaagcacca gcacctgatt aagcaacggc
    19861 gcctctgcca caatccccag cgtgatggcg acgatttccg cagacaaaat aaaatcggta
    19921 cgaatcgccc ctttaatttt gtccttttca aacttcagcg gatcctgcgc cgccagcttc
    19981 tccagacgct gctggctctg cgctggatct tctttatgtt tacgcgcctc cagcatatgc
    20041 agcactttct ctaccccttc aaagcagaga aacgcgccac caatcatcaa cagtggcgta
    20101 atcgcccacg ggataaacgc actgatgatc agcgccagcg gcaccagaat cactttattg
    20161 atcagcgatc ctttcgccac gccccagacc acgggaagtt cccggttggc ccgcacgcct
    20221 gaaacttgtt gcgcattgag cgataaatca tcccctaata caccggcggt tttcttcgcc
    20281 gccagtttgc ccatcacaga gatatcgtcc agcagtgtgg cgatatcatc gagcaacgtc
    20341 agtaaactac tcccggccaa aagcattccc tctcattttt attgttgaag ggtgtaagta
    20401 tgaagcaaaa acggcagctc tgatacttgc ttcacaggtc aacatttatt taacaatcaa
    20461 gtgcaattat atctctcgcc agcagaatga ttttcacgtt tactatgaac caccttttta
    20521 tttccgccgt gagggattat gcgtttccgc cagttgttac cgctttttgg cgcgctgttt
    20581 gcgttgtata tcatttgggg ctcaacctat tttgtcattc ggattggcgt gggaagctgg
    20641 cctccgttaa tgatggcggg cgttcgattc caggcagccg gtattttatt gctggcattt
    20701 ttgctactgc gcggacacaa acttcccccg ctacgtccgc tgctcaatgc cgcgctgatt
    20761 ggcctgttat tgctggctgt cggtaatggc atggtgacgg ttgccgaaca tcaaaatgtt
    20821 ccttccggca tcgccgccgt agtgattgca accgtgcccc tctttaccct gtgcttcagt
    20881 cgcctgtttg gcattaaaac gcgcaaactg gaatgggtgg gtattgccat tgggcttgcc
    20941 ggaatcatca tgctcaatag cggtggaaat ttaagcggca atccgtgggg cgcgattctg
    21001 attttaatcg gctcgattag ctgggcgttt ggctcagttt atggctcgcg cattacctta
    21061 cctgtaggga tgatggcggg tgcgattgag atgctggcgg caggcgtggt gttaatgatc
    21121 gcatcgatga ttgcgggtga aaaactgacg gcgctccctt ccctgtcagg cttccttgcg
    21181 gtcggctatc tggcgctgtt tggttcgatt atcgccatta acgcttatat gtatttaatc
    21241 cgtaatgtca gtccggctct cgccaccagc tacgcctacg ttaacccggt ggtcgcggtc
    21301 ttgctgggta cgggactggg tggcgaaacg ctgtcgaaga ttgaatggct ggcgctcggc
    21361 gtaattgttt tcgcggtggt actggtcacg ttgggaaaat atctcttccc ggccaaaccc
    21421 gttgtcgcgc cagttattca ggatgcatca agcgagtaaa tgaatcccct gcgtgtcgat
    21481 ctgcgcgctg gcaccttcgc cgcagatcca ctcttccaga cgctcggtaa gcgccgcatc
    21541 cgtcagcttc tcgcgcctac gtaacgcaca ctcccagaca atcagtacgc gccagccgag
    21601 ctcctgcaag cgactgatat cgcggcgatc gcgttcaaca tttttaccta tcttctccag
    21661 ccagaactca gttcgagtcg caggcacttt aaacagatag cagtgatgat gatgccagaa
    21721 gcagccgtgg gtaaatatca cgcagcgata ttcatcaacg acaaaatccg ggcgtccggg
    21781 cagactggcg tcctgaacgc gaaacgcaag gccctgcccg gttaacaaac tggcgaggcg
    21841 cttctcaatc gccgtatcac gcgtggcaat cgcgcgcata tttttgctgc gggtggcctt
    21901 atcgtgaacg tcgaccatgt tgtgcctctt gctgacgcaa cgccaccgcc tgtttgattt
    21961 ttggctcaag cagttttgcc accgcggcaa agaccggcac gaccaccgag ttgccgaact
    22021 ggcgataggc ctgagtgtcc gaaaccggaa tgcggaattt cgcttctccc ggcgcttcaa
    22081 aacccattaa gcgcgcgcat tcacgaggcg ttaaccgacg tggacgatgt tgctgattca
    22141 gcggatcgtc aaagtctttc tcacccgtgg ccatatccca accgcgatcg attaaaattt
    22201 ccgcgccatc tttgtaataa cgagcagaca gcgtacgggt gacgctttgc ggattgttcg
    22261 gataaaccat tccataaccg aagccgttac cgcgcgcctg atgttttttc gcatatcgat
    22321 agaggtactt ccacagcacc ggcgtcagga tatatttcgc ctcaaccatc gggtccagca
    22381 gctgcgccag cgtcactcgc tgtgcaggga aacattcgct gatatcacgc agagtaaaat
    22441 cggctttcag attcagatcg cgacgaaaac ccaccagcac gatgcgttca cggtgctgcg
    22501 gcaggaaatg tttaccatcg atgattttcg gatcgtccgg cccgttatct tctgcatcgg
    22561 caacgtcata gcccagttcg tccagcgtct gcatgatgat gcggaacgtt tttcccttat
    22621 cgtgactctt caggtttttg acgttttcga gcacaaacat cgccggacga cgcgcgtcga
    22681 taatgcgcac cacatcaaag aacagcgtgc cctgggtatc gcaggcaaaa ccgtgcgccc
    22741 gcccgagcga gttctttttc gatacgccag ccagcgaaaa tggctgacaa gggaaaccgg
    22801 ccagtaaaac atcgtgttca ggaatgtgtt gacgaatatg ttccgccgcc gcctcatcac
    22861 tcacgccttc ttgatgactg agggtgatgt cgcggatatc ttcattaaaa tgatgcgtcg
    22921 ccgggtcgca ataatggttg gctttataag tgcgtaccgc atgtttgttc cattcgctgg
    22981 taaacacgca ctgtccacca atcgattcaa aaccacgacg gatgccgcca attccggcaa
    23041 atagatcgat aaagcgaaac gcataatgcg gatgatgttc cggtggtttg ggtaacaacg
    23101 tttgcagatg ggcaaactct ttctcactta aacggtgcca tgccgagtca ttcgccagcg
    23161 cacgttttaa aattgccgcg ctccagtgat tctcacccac accattaagc tgcgccacca
    23221 gcattttaac atcataaatt tgcagcagtt tctccagcat tgcctgtgcg gcattcccgg
    23281 tgctgtatga atcggttact gatatatttt cctgcataga tttaaccggc catctgagat
    23341 ggccgacagg ttaacataat tactgccgtt tagggccacg cgctcagcca gcgggtgaaa
    23401 acgcatctat caccttgtga tcgatacctt cattctcccc cgccagttcg gcaccgagct
    23461 tcgccatcaa acagcgccaa gctcaatgcc cctgaaacgg caaatacgcg cgccaggcca
    23521 atcgccccca gggcttccag ccgatccgca tcctggacga ttttagcttc cgttgttaag
    23581 ggggcgattt gcgcgctgaa actgtgagcg gcaatggcat gacaaacggc ctcaattttc
    23641 tccgccggaa attgcacgaa ctcttcacgg agcaggcgac gcgtctcttc tgccgccagg
    23701 attgaagaac gctgccgttg cggatgattt ttcgccaggc tcacgatatc gtgaaaataa
    23761 caggcggtta aaatcaccag catatcgaca tcgtcatctg ccgccagttt ctgtgccgta
    23821 gcccagacac ggcgaaaatg acacaggtca tgcgccgcgt cctgatgttg atgatggttt
    23881 ttcaaccagt tctcgaacta tgcctgccag tgttgtaact ccatacgcgc tccgttgcca
    23941 aagagtctca accttagcag tttttattca tagcgtctgc aacgtgcggg acgaggtcgg
    24001 gtgtaccatg caatgccgcc taacaaggca ccaaccaggg ccataaacag aaatccgacc
    24061 aatgctccta accacttccc cgcggtagat cctaaatcac tttcaaatcc cgacacgggg
    24121 gcatcgggga tccggctcat cacccagatg tatcgcgcca ttgcccagac gaaatagcca
    24181 caccatgtat agaaagccc
//
LOCUS       DAZXUK010000034        23232 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZXUK010000034 DAZXUK010000000
VERSION     DAZXUK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23232
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.034"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..343)
                     /locus_tag="ACH3L8_001127"
     CDS             complement(<1..343)
                     /locus_tag="ACH3L8_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084331.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
     gene            693..1715
                     /locus_tag="ACH3L8_001128"
     CDS             693..1715
                     /locus_tag="ACH3L8_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFT4084332.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1715..2695
                     /locus_tag="ACH3L8_001129"
     CDS             1715..2695
                     /locus_tag="ACH3L8_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFT4084333.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2692..3450
                     /locus_tag="ACH3L8_001130"
     CDS             2692..3450
                     /locus_tag="ACH3L8_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFT4084334.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            3460..4269
                     /locus_tag="ACH3L8_001131"
     CDS             3460..4269
                     /locus_tag="ACH3L8_001131"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFT4084335.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            4269..5123
                     /gene="modD"
                     /locus_tag="ACH3L8_001132"
     CDS             4269..5123
                     /gene="modD"
                     /locus_tag="ACH3L8_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFT4084336.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            5149..7103
                     /locus_tag="ACH3L8_001133"
                     /pseudo
     CDS             5149..7103
                     /locus_tag="ACH3L8_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(7153..7407)
                     /locus_tag="ACH3L8_001134"
     CDS             complement(7153..7407)
                     /locus_tag="ACH3L8_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFT4084337.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7608..8342
                     /locus_tag="ACH3L8_001135"
     CDS             7608..8342
                     /locus_tag="ACH3L8_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFT4084338.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(8344..8955)
                     /gene="emtA"
                     /locus_tag="ACH3L8_001136"
     CDS             complement(8344..8955)
                     /gene="emtA"
                     /locus_tag="ACH3L8_001136"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFT4084339.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            9055..9969
                     /gene="ldcA"
                     /locus_tag="ACH3L8_001137"
     CDS             9055..9969
                     /gene="ldcA"
                     /locus_tag="ACH3L8_001137"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFT4084340.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            10064..11800
                     /locus_tag="ACH3L8_001138"
     CDS             10064..11800
                     /locus_tag="ACH3L8_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFT4084341.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            12411..13825
                     /locus_tag="ACH3L8_001139"
                     /pseudo
     CDS             12411..13825
                     /locus_tag="ACH3L8_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13993..15063)
                     /gene="dadX"
                     /locus_tag="ACH3L8_001140"
     CDS             complement(13993..15063)
                     /gene="dadX"
                     /locus_tag="ACH3L8_001140"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFT4084342.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(15073..16371)
                     /gene="dadA"
                     /locus_tag="ACH3L8_001141"
     CDS             complement(15073..16371)
                     /gene="dadA"
                     /locus_tag="ACH3L8_001141"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFT4084343.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16701..18234
                     /locus_tag="ACH3L8_001142"
                     /pseudo
     CDS             16701..18234
                     /locus_tag="ACH3L8_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
     gene            complement(18286..19005)
                     /gene="fadR"
                     /locus_tag="ACH3L8_001143"
     CDS             complement(18286..19005)
                     /gene="fadR"
                     /locus_tag="ACH3L8_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFT4084344.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            19226..20767
                     /gene="nhaB"
                     /locus_tag="ACH3L8_001144"
     CDS             19226..20767
                     /gene="nhaB"
                     /locus_tag="ACH3L8_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFT4084345.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            20913..21443
                     /gene="dsbB"
                     /locus_tag="ACH3L8_001145"
     CDS             20913..21443
                     /gene="dsbB"
                     /locus_tag="ACH3L8_001145"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFT4084346.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(21489..22757)
                     /gene="umuC"
                     /locus_tag="ACH3L8_001146"
     CDS             complement(21489..22757)
                     /gene="umuC"
                     /locus_tag="ACH3L8_001146"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFT4084347.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(22757..23176)
                     /locus_tag="ACH3L8_001147"
     CDS             complement(22757..23176)
                     /locus_tag="ACH3L8_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFT4084348.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         5596 a         6079 c         5767 g         5790 t
ORIGIN      
        1 ggagaatgtt atcattgcta caccctgtgc ttaataactg caactctccg ccgttcagcg
       61 atgctttgat aattctgttc ccatagatat cgttatcaaa ataaagcgta aatatcatat
      121 ttccgtaact gttatcatct ctttttttgc acgtccgaat aaacgcattc agtaaattac
      181 tctctctaag aaaataaata tcttcattgt tgttaatgac attaataacg tcgttcttaa
      241 aatcctcaaa gtcttgttgc ggtaacttct ggttacgctg cttactatca gcaagcggtg
      301 aatccggacg acaacaacat aggttttgta taacccccag cattcttttc acctccgtta
      361 acagtatatt cacgacactg aaacgagaga atgctgataa ttttctcggc gagatatcaa
      421 aaatcgctca ttataaaaca agtaacttca acgtcgcgta accgtggacg ataaaaaaac
      481 aataatgtca ttcatattta aataatttag ccttaaatga tttaaataat attagccacc
      541 tgataaatac atttttaatg atagagaggg tcaacaggct tgcggaagtc gtcacatctt
      601 catgttttca atagctaaac tttgatattg aacatgattt ttatcgatat atatgaaagt
      661 atatatcgat tttacttttc aggatgttca ccatgcccat aaccagacgg acgtttgcgc
      721 aggcacttgc atctaccctg ctcctgcagt ctctaccgtc cttttcacaa acagttaatc
      781 gttttgcttc gcaatctctt cccgaagccc aaaacattac acgcatcgtc agcgcaggcg
      841 cgcccgccga tttgctcttg ctggctgtcg cgccagaaaa aatggtcggt ttttcctctt
      901 ttgattttgc ccgtcaggca ttaattccat tgccagagca cattcgccag ttacccaggc
      961 tgggacgact cgccgggcgc gccagcacac tatcgctgga agggcttatg gcgttacatc
     1021 ccgatttggt cgttgattgc ggcaatacgg atgaaacctt gatctcccag gcacggcagg
     1081 ttagcgaaca gacacaaatc ccctggttat tgcttaacgg aaaactggcg caatcagcag
     1141 aacagttaac aacgcttggc aaaacgttag gcgaagagca ccgcgccgca gaacaagcca
     1201 atctcgccag ccacttcgtt ggtgaagctc aggcattcgc cacctcaccc gccgctaacc
     1261 tccgctttta tgctgcgcgc ggtccccgag ggctggaaac gggcttacag ggttcgttgc
     1321 ataccgaggc ggcggaatta ttaggtttgc acaacgtcgc gcaaatagcc gatcgccacg
     1381 gcctgacaca ggtttccatg gaaaatctcc tgcgctggca gccggatatt attctggttc
     1441 aggaggccgt tactgcagat tttattcgtc gtgatcctct ctggcagggc gtgaaagccg
     1501 ttgcggaaca acgcatctta tttttaagtg gcttgccctt tggctggctc gatgccccgc
     1561 cgggaatcaa ccgtcttctg ggattacgca gacttcacgc ctggctggat cccgccatca
     1621 atcgccagtt taaaagtgac atgcagcatt acgcccaact gttctggcat tgttcactca
     1681 gtgatgccga ctatcaaaaa ttggtggcga gctaatgaga atcgtaaacg gatgtatttt
     1741 gctgctggca gctatcagta tcacttttgc cgccgtaagt ggggcttatc atctcgatat
     1801 gcagcaactg ctggcgctaa ttcttcgtca agaaaatgag tctgtgcagg aacaaattgt
     1861 cttctggcaa atccgcgtgc cgcgtattct tgctgcgctg ttactgggcg cagcgctggc
     1921 aggagccgga accacctatc agggaatgtt gcgtaatccc ttagtttcac cggatatttt
     1981 aggcgtctcc gccggtgccg gacttggtgc ctgcgcggca atcctgtggg gattacccat
     2041 tgtttacatc cagctgtacg cattttgcgg cgggctgatg gtggtcgcgg gcgtctggtt
     2101 gattacgcgt cgggtgaccc gccacgatcc gatcttaacc ctggtgctgg taggcattgc
     2161 gttaggaacg ttgtgcggag cggggatatc gctaattaaa acgctggccg atccttacac
     2221 ccagttaccg tcaattacct tctggttgct ggggggactg tccaccgtga ccttacgcga
     2281 tctctgctat gctgcaccga tcattctcat aggctccttg cccctgtttt ttctccgctg
     2341 gcgcatgaat ttactcacgc tttccgacga cgaagcccgt tcgctgggac tgaacgtgac
     2401 gcgtctgcgc tttggattga ttgtctgcgc cacactaatc accgccagca cggtagcaat
     2461 agcgggcata attggttgga tcggtctggt cgttccgcac attgcccgct tgttgaccgg
     2521 tcataaccat cagcagctgt tgcctatggc aatgtgtact ggcgctattc tgctcctgct
     2581 taccgatacg ctggcgcgca gcattggaac aacagaaatc ccacttggca ttctgaccgc
     2641 ttttgttggc gcaccgtttt tcctgctttt gttgttacgc ggaggtcgcc agtgactttg
     2701 cttgccgttc gtcatgcgtc tctgggttac tcccgccatc cagtgctacg cgacgtgtcc
     2761 ttcaccctgt cgcaaggaac aatttgttgt ctgcttggcg cgaatggttg cggcaaaacc
     2821 acactcatgc gctccattct tggcgttata ccgttattaa aaggcgagat cctactggat
     2881 agcctctctg tgcagacact cagccatcgc cagcgcgcac aggctattgc ctgggtgccg
     2941 caggcgcacg acggtatttt tgctttcagt gtgctggata tggtgttgat gggcctggcc
     3001 cccaccatag gcgctttttc cgttcccggt aagcaagaac gcctgaaagc cacggagcaa
     3061 ctggaaaagc tgggcatttt gcatctggcg acacgacgct ggaatacgct tagcggcggt
     3121 gaacgtcagc tggctttaat cgcccgtgca ctcgtccaac aaccgcgctt gttgttactg
     3181 gatgagcccg cctccagcct cgatttcggt catcagatcc aacttctgga cacgctggcg
     3241 cagctcaaaa ataacggcat gacaatgtta atgtcgactc accatacgct ccatgccaac
     3301 gccattgccg acagcattat ccaagtagag ccagatggac gagtaacaca aggcttaccg
     3361 accgaacagt taacaacgaa caaactggcg gccctgtatc gggtttctgc cgaccaaatt
     3421 catcatcacc tctctgcaat tagccgctaa ggaacaatca tgctcattga tgatattgat
     3481 tttgcagacc tttatctgca gcaactcaaa ctggctcacc gcacagaaaa aacgcccgat
     3541 cactgggatc aacgggcgga gaaaatggcg gaaaattgcg cctcgccaac ggacagctac
     3601 ctgcaacaac tgatcgctaa aattgacctg caaggcgctc agaccttatt cgatatgggc
     3661 tgcggaccag gcaccgtatc gctggcgctg gctgacaaac tcactacggt ttatggcgtt
     3721 gattacagtc aggggatgct gaacgttgcc gcccgccgcg ccgccgcact gaaggctgac
     3781 aatgtccact ggatccagcg cgcctgggaa gaggactgga gtgacctgcc acgctgcgat
     3841 attgccgtgg cttcccgctc cactctggtg gctgatatgc gccatgcaat gagcaaactg
     3901 aataatcagg cacggcttcg cgtctataca actcatctgg tcagcacctc gttcgtctct
     3961 ccagccattc agcgggcggc tggtcgggaa gtgatcgaat tacccaacta tatctttgcg
     4021 ctcaacgtcc tgtatcagat gggaatttat gcccacgttg attttatccg tggacaaaat
     4081 tgccaacagg ataacagcac ctgggaacgt tttgagcaga acgtcagctg gagtcttggc
     4141 gcgcttaacg acgatgagcg tgagcggtta tatcactggt atcaacaaca agatgctcgt
     4201 gccctcgccc ctgccagccg cgactgggcg ctcatctggt gggatagcgt accgcaggag
     4261 gcactgtgat gattttcctt tctcaggcac aaatcgatgc gttactgctg gaagatatcc
     4321 agggaggtga cctgaccacg cgagcattaa atattggaca ccagcctggc tatatagcgt
     4381 tttttcaccg tcagggcggc tgcgtcagcg gcgtttctgt cgcgtgtaag atgttaacta
     4441 cgctgggatt aactattgat gacgcggtca gcgacggttc acaagcgaac gcgggtcaac
     4501 ggctaatccg tgcgcagggt aatgccgcag cacttcatca gggatggaag gcagtccaga
     4561 atgtgctgga gtggagttgc ggtgtttctg attatctcgc tcaaatgctg gcattgcttc
     4621 gtgaacgtta tcctgacggc aatatcgcct gcacccgaaa agcaattccg ggcacccgct
     4681 tactggcttc gcaggcaatt ctggctgccg gaggactaat tcatcgtgcc ggatgtgcgg
     4741 aaaccatatt attgtttgcc aaccaccgcc attttcttca tgacaatcag ggctggtcag
     4801 gcgcaatcgc tcagttacgt cgccacgcac ccgagaagaa aattgttgtt gaagccgaca
     4861 cgccgaaaga ggctatcgcc gcgttacgcg cacaaccaga cgtacttcag ctcgacaaat
     4921 ttagtccgca acaggcaact gaaattgcgc aaatagcgcc gtcgctggct cctcactgca
     4981 cgctggctct gaccggcgga attaatctga cgacactcaa aaattacctc gactgcggca
     5041 ttcgcctttt tattacctcc gcgccttatt acgcggcacc agctgacatt aaagtcagtc
     5101 tgcaacccac agcctctatt taattctttg ttaaacggaa tttattctat gagactcaaa
     5161 aaacgttatc tctgcacagc attatcactt gcctttaccc agcaggccgt agcggctcag
     5221 gagagcgaca cgctgaccgt atggtccagt ccggtatcat cgacgacgac caccgttctc
     5281 gatcaaccca ccatgaaggc cttggataaa cagaatgtcg ctcaggcatt aagtgtcgtc
     5341 cccggcgtgg tgctgcaaaa gtcaggcagc cgcaacgaag aacaggtaaa agttcgtggc
     5401 tttgatagtc ggcaggtgcc ggtctatttc gacggtgtgc ccatttatgt cccctatgac
     5461 ggcaacctcg atctggcgcg gattctgacc aacaatctgg gggcagtcga agtttccaaa
     5521 gggtattcgt cgctgcttca tgggcctaat cagatgggcg gagccattaa tatcaccacc
     5581 ctgaagaagc aagtctggga tatcgccagg gatggagccg tagtcaggac aatgcctacg
     5641 atatgcatgc ttcatttgcc gccagcagcg atcttgggta tttgcaagtc agcggtagcc
     5701 agctaaagca ggattttctc ggcctgccgc atggtgtaaa taatgatatt gcaggcaaac
     5761 acggcaagat gattaattca tcggctgatg ataaacgcgg cattgtgaag ctcggtttta
     5821 caccgcgcga aaacgatgaa tacacgctga cttacattaa gcaggatggt gaaaaagata
     5881 acccgccata cagcggaaat tgtggtcaaa aatcacgcta ctggcagtgg ccagagtatg
     5941 acaaagaaag tttttattat cagggagcga cccaactaaa cgaccgtttt accctgaaaa
     6001 gtcggctgta tcgcgacacc tttgaaaata cgctgatgat gtacaactcg ctggctgatt
     6061 tgaaaaataa aaaaggcagc tacagccatt attccgatta cagcgacggt gccggattac
     6121 aactggcagc cgatgtgcgt gaaaacgatc tgctgtcgtt tgccgttaac tggaaagatg
     6181 acgtacaccg ggaaaaaggt gcgccgcacg ccgcttacga tcgctatgaa gatcgtacct
     6241 ggtcgctcgc cagtgaatat caatgggctg ctgctgataa tgtcgatgtc gtggcgggaa
     6301 tcagctatga ctggcgcgat agcgtagaag cgaaaaaaca cgagaaagat ggcagcatca
     6361 cccattatga cgacaacaat cagtcagctt ttaactggca ggtgatgggg aaataccact
     6421 ttgccaatga agacactctg gcgctttcgt actatgaccg cacacgcttt ccgacgctga
     6481 aagaacgcta taccacatcc aaacctgcgt ataaccagat agcgattgtt aacccgcagc
     6541 tcaagccgga acgcgctcgc ggggtggatt taacctggaa tggtgccttc acgcgcgact
     6601 ggggatttga ggtcagcgtt tactataacc gggtgagtga tgccatcctc tcgcacaata
     6661 tcaatgccga taccattcaa aatcagaaca gcggcacggt ggattacagc ggtctggatg
     6721 ccggtattaa ggggaaaatc agcaatatac tggatgtagg attgagctac gctctgatcc
     6781 acgctgacgc caaacgcaaa gacatcggca agataaccga tcttccaacg cagacaatga
     6841 ccgcatggat gacactcaaa ccatgggagc cgttaagcgt aacgctgtcg gaagaggcgc
     6901 gttcctccag ctacagcaac agtgacggtt cacaaaaagc cgccggtttt gcggtgaccc
     6961 acattcgagc cgattacacc ttaggtcatg gcttcagcgt caatgcgtcg gtcaataacc
     7021 tgtttgatac ccaatatgcc tacagtgaag ggtttattga agaaggtcgc aatttctggg
     7081 caggtgtcga gtatacgttc tgatgtgaaa gcggatagcg gcatctgtat cgctatccgc
     7141 cttatgaaaa tcttattctc ttcgcaggag gcggaatacg cccagcacca ggatagcgcc
     7201 caccaccgcc accaggaagc tgtgcaaatt aaaaccgctg atggagccgt caatgccaaa
     7261 catggtcgcc agccagccgc cgaccaccgc accgactatc ccgagaatac aggtcaggaa
     7321 aaatccacca ccatcacgcc ccggcatgat tagcttgccg ataatgccgg ctatcaggcc
     7381 aaaaataatc caggcaataa ttcccatgtt caggccctcg tttcgatgaa tcggtcgcaa
     7441 gaaatgctta cgtcaacgct ctaagtatag gcaagggcgg gatcgatgct gggcgtttgt
     7501 cggctgattt aagaacaaca tgtaaaaaat attcacgcat ttgtattaag ttttgttaac
     7561 tatgaccgat aaaccaaaga cagtttgtca gtcaggagtt tttccgcgtg agtcattacc
     7621 atgagcagtt cctgaaacaa aatccgttag ccgtcctggg cgtgttacgc gatttgcaca
     7681 aagccgcaat tcctttgcgt ctcagttgga atggcgggca gctgatcagc aaaatactgg
     7741 caataacccc ggataaactg gtgctggatt tcggcagtca agccgaagac aacatcgccg
     7801 tgctaaaggc acagcacatt accattaccg ccgaaactca gggtgcgaaa gtcgagttta
     7861 ctgttgaaca actacagcag agtgaatact tgcagcttcc ggcatttatt accgtaccgc
     7921 ctcccacctt atggtttgta caacgacgcc gatatttccg catctccgcc ccactccatc
     7981 cgccttattt ttgccagacc aaactggcgg ataacagtac gttacgtttc cgcttgtatg
     8041 atttgtcgtt aggcggcatg ggcgcattac tggaaacagc aaagcctgcc gaattacaag
     8101 aaggtatgcg cttcgctcaa attgaagtca acatggggca atggggtgtt tttcactttg
     8161 acgcccagtt aatctccacc agcgagcgca aagtgattga tggcaagaat gaaaccatca
     8221 ccactccccg tctgagcttc cgttttctta acgtcagccc gacggtggag cggcaattac
     8281 agcggattat tttctctctc gagcgagaag cccgggaaaa agcggacaaa gtgcgcgact
     8341 gatttacatc gcgtccagtg cctgctcaag tttgtagata tagcgcggag cctgcggcgc
     8401 aggatgattt cgcgctacgt gatcgaggaa ctcgtcagca tccaaatcgt tgattttgct
     8461 gatcgccttt ttccgatctg acgagaaagt ccgtagcagc gcacctgccc cgttagcgta
     8521 tgacaccacc agcgcatatt gcagtacctt cggatcttca atgcctgcca gcgggccggt
     8581 ttccagaata ttcaggtaag ccgcccccat tgaaatatta cgctctggat ttttcagctc
     8641 gctggtcgtc ggctcaccac tccagcccat acggcgataa acatcacgtc cggaggttga
     8701 agcttttaac tgcatcaaac caatggcatt cgatttactc accgcgttcg gattaccacc
     8761 cgattcgata gcgataatcg ccgtgatcaa ttgtggatcg acgccccagg ctgcaccggc
     8821 tttctggctt attggcatcc actgcatcgc acgttgcacc ggaactttcg cgttccacgg
     8881 cgggttcgta tagtcatgct ttgatgaaca acccgctaat aacacaatca aaaaggcaaa
     8941 ccatctcaat ttcacttctt ctatccttat agcctggaat cgccgttgag gtgacaacgg
     9001 cacacctaag cggcatgata tacatttcgt ttctgttgtc agcaaggaat tgccatgtct
     9061 ctgtttcact taattgcccc atcgggttac agcattaaac agcacgccgc gctgcgtggt
     9121 atccagcgcc ttaccgacgc ggggcatcaa gtcaataacg tcgaggttat tgcccgtcgt
     9181 tgtgagcgtt ttgcgggtac ggaaacggag cgtctggaag atctcaattc ccttgccaga
     9241 ctgactaccc ccaacaccat cgtgctggct gtacgcggcg gttacggtgc cagtcgttta
     9301 ctggcagata ttgactggca ggcactggtg gctcgccaac aacatgaccc gttactcatt
     9361 tgcggacata gcgattttac cgccattcag tgcggtcttc tggcgcaagg caatgtcatc
     9421 acctttagcg gccccatgct ggtagcaaac tttggtgcgg atgaactgaa cgcctttacc
     9481 gagcaccatt tctggctggc gttacgcaat gaaaccttca ccattgaatg gcaaggggaa
     9541 ggcccgacat gtcagaccga aggcacgttg tggggaggca atcttgcgat gctgatttca
     9601 ctgattggta caccgtggat gccaaaaatt gagaacggta ttctggtgct ggaagatatt
     9661 aacgtgcacc ctttccgcgt cgaacgtatg ctgttgcagc tctatcatgc gggaattttg
     9721 ccacgtcaga aggcgattat tctcggtagc tttagcggca gcacgcccaa tgattatgac
     9781 gcaggttaca acctggagtc agtgtacgcg tttttgcgtt cccgcctgtc gattccactc
     9841 attaccggcc tcgattttgg tcatgaacaa cgcacggtta cgctgccgtt gggcgcacat
     9901 gccattctga ataacacccg ggaaggcgct cagttgacga ttagcggtca tcctgttctt
     9961 aaaatgtaaa aaaatcacgc ttcagggcta agtaaaacgc tgtctctgcc gctaatatgg
    10021 tagggtttat ataagagaca gcgtaatcag gagtaaccga ccattggatg ccacaacaat
    10081 aattagcctt ttcatcttag gatccatcct tgtcaccagc agtatattac ttagttcatt
    10141 ttcttcccgt cttggcattc ctattctggt tatttttctg gcgatcggca tgctggcggg
    10201 agtcgatggc gtcggcggta tcccgtttga taattacccc ttcgcctaca tggtaagtaa
    10261 cctggcgctg gcgattattt tgctcgacgg cgggatgcgc actcaggcca gttcctttcg
    10321 tgtggcgtta ggaccggcgc tgtcgctggc gacgctgggc gtacttatca cctctggttt
    10381 aaccggcatg atggcggcgt ggctgtttaa tcttgatttg attgaaggct tattaatcgg
    10441 cgctatcgtc ggctccaccg atgctgcagc ggtcttttct ctgctgggtg gtaaggggct
    10501 taacgaacgt gttggctcga cgctggaaat tgaatccggc agtaatgatc caatggcggt
    10561 ctttctgacg attaccctga ttgcaatgat ccagcagcat gaaagcagtg tcagttggat
    10621 gttcgtggtc gatattctgc agcaatttgg cctcgggatt gtcatcggtc ttggcggtgg
    10681 ttatttactg ctgcaaatga ttaatcgcat cgccctgcct gccggattat atccattgct
    10741 ggcattaagc ggcggtattt taatttttgc tttaaccacg gcgctggaag gcagcggtat
    10801 tctggctgtt tatctgtgcg gttttctgct gggtaatcgg ccgattcgca accgctacgg
    10861 catcctgcaa aatttcgacg gcctcgcctg gctggcgcaa atcgccatgt tcctggtgct
    10921 ggggctattg gttaacccaa gcgatctgct gcctattgcc attccggcgc tcattttgtc
    10981 ggcatggatg atattcttcg cccgtcccct ttcggtattt gctggattgc tccctttccg
    11041 cggtttcaat ctgcgtgagc gcgtgtttat cagctgggtg ggattacgcg gcgcggtgcc
    11101 aatcatccta gccgtgttcc cgatgatggc ggggctggag aatgcgcgac tgttctttaa
    11161 tgtcgccttc tttgtggttc tggtttcact gctcttgcag ggaacatcac tctcgtgggc
    11221 ggcgaaaaaa gccaaagtgg tggttccgcc cgtcggacgt ccggtgtcac gcgttggcct
    11281 ggatattcat ccggaaaatc cgtgggagca gtttgtttat caattgagtg ctgataaatg
    11341 gtgtgtaggt gcggcactgc gcgatttgca tatgccaaaa gagacgcgta ttgcagccct
    11401 gtttcgtgat aaccagttgc ttcatcctac cggcagcacc cgactgcgcg aaggcgatgt
    11461 attgtgtgta attggtcggg aacgcgatct cccggcgctc ggtaaactgt tcagccagtc
    11521 gccaccggtc gcgctggatc aacgcttctt tggtgacttc attctcgaag ccagcgccaa
    11581 atatgctgat gttgcgctga tatatggcct ggaagacggg cgagaatatc gtgataaaca
    11641 gcaaacgctg ggtgaaattg tccagcagtt gctaggcgcg gcaccggttg tcggtgacca
    11701 ggtagagttt gccgggatga tctggacggt ggccgagaaa gaagataatg aagtgttgaa
    11761 gattggcgtt cgggtggctg aagaggaagc tgaatcttaa atttaatgca gcatctatcg
    11821 ccggatgcga tgctttactg aacgcagcct gagcctgtaa taaacttgtc atttcctgat
    11881 tttgatatgc tcatcccaac atcagaagaa ggttaaatta tggatgacaa accattgcag
    11941 gctctggcta atgaactcaa aacaccggct caagaaagtc agcgtaaggg ctttttccga
    12001 ctgtaacagg taataaaaac cagccccgca ttaccggtca accatcaggg gcccctgcct
    12061 gacatcgcct gaacatattc ccccacgcaa cctgccgcac tgacatagcc cacacaatag
    12121 gcgtttcatc cgcctgtagc accggatgca tgagcgattt cttatagtta ttcctttgct
    12181 taataacgat tatttccggg tcgttcactc tcgtattttc tttaatacgt cagagctacc
    12241 taggccgtaa tacagcatgt accagacccg acacggcatt tctgaccagg aaaaaagtca
    12301 acgatgagta gtgatatcag cgcaaatgac gtcctgtggc tcctttgcgg tgaccacatt
    12361 cacgtctccg gcgctgccag aggatattta tttcaaggaa acgtagcatt atggctagct
    12421 ttaaagacat ctgcccattc gcccccgcca ttcccccatc ttccgttaac ccaggcgtac
    12481 gacgcccgcc agggcggctc ttctcaagaa tcaaattttg cagaacatga agtatctcag
    12541 cccgcaaaaa ttcagctggg gtgatgcccc ctggcagatc attgacctca gtatcgcggg
    12601 caaagtgaac atacaggtgg acaacaatac catcatcacg ttggggactc gtctgaatca
    12661 gcaacataac gagttcatga tggtcgccaa atggtgcgaa tgggctatac agcaggatgg
    12721 tttgcaagaa aacctgcaaa aaaacctgta cgaaatcctg gaagaaaacc agcaaaacaa
    12781 acagtcagaa attccccaag aagacctgaa agaaagtctg gaagaaatca aggaaaatat
    12841 cctggaagaa aacctgccag caagccggat tgaaaaccgg gcagaagcat tgcgcagaat
    12901 gaaggaatgt ctcattacaa gacggagtat gctcaatctt agcaacctgg gattgacttc
    12961 actccctgag aatttgcctc cacatctgat tgaattttac tgcagtaaaa acgtgttgac
    13021 cgcgttaccg aaggtgatgc caaagtggct gctggtgctt gactgtacag ataacgtctt
    13081 gatcttgtta ccgaaggtgc agccctcaaa actgatggta ctgaagtgct atgaaaacag
    13141 tattatctgg ctgcctgagc tgtcgactaa cctgagagtg attaactgtt ctgaaaactt
    13201 cttgcaattt ttaccgccgt cgatgcccca gtacctgtat aaactatcct gtgccggcaa
    13261 caatattaac tcaatacctg atgagatgct ggagaacttg actcgcctga aagtatttga
    13321 ctgttctagt aacgatttga tctcttcacc acggctgccg cccaaactaa taatttacta
    13381 ctgtggagaa aacaagttta aaacggtaca ggtgccgcag ccccagagcc tgaaggtgtt
    13441 tgactgtaat ggtaacccgt gggacaaaga caatttaccg acgctgctca aagccgtcga
    13501 gggcctgaaa aaacagcagg gcctgaagga ccttttggac tttttgcaca aggaaggcta
    13561 ggttgacctg gaaggactcg aggagctgga ggaccttatg gatctggagt tcctggatga
    13621 cccggaactc ctggagcgcg tgaaggtaca ggaggacctg gagctcctgg atcaacagtt
    13681 gggcctgttg agtctggaaa gacaacagga ctcgcagcct gttaatcaac aatctgaaca
    13741 tgaacccgaa tctgcatcaa gggtgaagcg tgatttatct gaagtcgact ccgagtcaac
    13801 aatgaagcgt aagcgtttta tgtagaacgc ctgtgcggcg agttcaggtt ccggggaaat
    13861 agtgtccatt cttttaatgg acactatcat gggcaatggc tctgtcacat taacaactac
    13921 agttgctgac cagccggatg gatgattttt tcgcaaccag aagacgctgc ctccgatccg
    13981 gcttacaaca agttacactg tcacaaccgg gacgcgtaac gccagtgcgc acatcaactc
    14041 atagcccacc gttccggcag cggcggcgac atcatcaatt ttgatctcct tgccccacag
    14101 ctcaaccggc gtaccaatcc ccgcctgcgg gcaaggcgtt aaatcaaccg ccagcatatc
    14161 catcgagacg gtccccaccg tcatggtgcg cacgccgtcc actaaaacag gagcaccggt
    14221 aggcgcatgg cgcggataac cgtcggcgta tcctgcggcg acaatgccga ttcgctgttc
    14281 atcgcgcgca gtatagcgac cgccgtagcc cacacgctcg cccgctttta gcgtctggac
    14341 accaataatc tcactgctta gcgtcatcac cggacgtaat ccggtattgg cgatatcacg
    14401 ccactgaccg gacggcgaag cgccatacaa aataatgcca ggccgaaccc agtcaaaatg
    14461 cgcttccggg tgccacagag tcgccgccga attggacaac gaacgccgac actccagccc
    14521 ctccgccgcc tgctcaatac gcgccatcgc gccggaaatt ccatcaggat gttccgcttc
    14581 agcaaaatgc gacatcaggg tcatttcgcc aacattcgcc attgcccgca actgctgcca
    14641 gacggtaagc acgcgatcgg actggaagcc caaccgattc atcccactgt tcactttaag
    14701 ataaatatcc aacggtgctt ttagccgcgc attttgcagt gctttgagct gccagttgct
    14761 gtgtacgcag gtggtcaggc ggtgctggtc ataaatctcc agatcctgag catggaaaaa
    14821 tccttccagc atcaggatcg gccccttcca gccgcgctca cgtaacgtta ttgcctcttc
    14881 caggttaagt aatgcaaagc catcggtggc cccgagcgcg ctccagatac gctcaatacc
    14941 atgcccgtaa gcgttcgctt ttaccaccga ccagacgcgc gcgtgcggcg cggcctggcg
    15001 gacaatggac agattctgtt ttaatgcctg cagatcgagg ctggcctgta tcggacgggt
    15061 catctcgttt ccttagctgt gtgcgccatg taaatggccc ggacgtgatg gcgtaaatcc
    15121 acggctgtag cgcgctacgc ttagatcctc atatgggatc gctggcgtgc gaccagagag
    15181 cagatcgctt aacaactgac cggaaccgca agccatcgtc cagccgagcg tgccgtgacc
    15241 ggtattcagc cacagatttt taaagcgtgt actcccgaca accggcgtac cgtctggcgt
    15301 catcgggcgc aaacccgtcc agaaagtcgc ctgctcgaca tgaccgccgc gtggatagag
    15361 atcgcgaacc accatttcca gcgtttcacg acgcggttgc aacagctcgg tgttaaaacc
    15421 aacaatttcc gccattccgc caacacgaat gcggttatcg aaacgggtaa tggcgatttt
    15481 gtaggtttca tcaagaatgg tggatactgg cgcaccatct tcctgcgcaa ttggaatagt
    15541 cagcgagtag cctttcagcg gatacaccgg aatatcaaca atgcctttga gcatcgccgt
    15601 cgagtaagaa ccaaacgcca tcacatacgc atcggcctta atcacttcat cgccaaactt
    15661 cacgccgtag atttgctcgc cgtcgcaaag cagttggtca acgggcgtat taaagcggaa
    15721 tttaaccccc gcctgctctg ccatccgcgc cagattctgg gtaaacagct ggcagtctcc
    15781 ggtttcatca ttgggtaact gcaggccgcc cgtcagtttg tgcgctactt ctgccagcgc
    15841 gggatccact tccgccaggc ggctggattc cagcagctga tacggtacgc cggcatcttc
    15901 cagcacggcg atatcgcggg tcgcattttc atactgttgt tcggtacgga acagttgcag
    15961 cgtcccaccc tgacgccctt catactgaat attggtttcg gcgcgcaatg ctttcaggca
    16021 atcacggctg tattccgcca gacgcaccat ccgccctttg ttttccatgt agtggctggt
    16081 gtcgcagtta cgtaacattt gccacatcca tttcaactga aactgcgtac cgtcgagacg
    16141 aaccgccagc ggcgcatggc gctggaacat ccatttaatc gcttttaaag gcacacctgg
    16201 tgctgcccac ggtgcagcat atccggggga gatttgcccg gcattcgcgg cactggtttc
    16261 cagggcggcc cccggctccc gatcaatgac ggtgacctca tgtcctgcct gatttaagta
    16321 ccaggcgctg gcaacgccta ccacaccact tcccagtatg acaactcgca tagccactcc
    16381 gataacggta gatacagtaa aagaataata atctaattac atcttgataa ctcagttgaa
    16441 aatattattc aacatacggc ttttttatgg tgagctgact cacatctccc tgactctgcg
    16501 gcaatatggc gtctttagta tctcctgctg aacgcccttt ttatccagca taaaattccg
    16561 ttcagaagcg gattagtggc actctgataa taagaaatcg caaagaaaaa aattctccgg
    16621 cgacgcggtt tttaacaggt gttctatgct tgaaatgagg tgctccacac agagagcgcc
    16681 gacaacaatg aggtgcgcgt atggcgacga tcgattctat gaataaggac accacacgtt
    16741 tgagcgatgg acccgactgg acgttcgacc tgctggatgt ttatctggca gagatagacc
    16801 gggtggcgaa actctaccgg ctggatacct acccgcacca gattgaagtg ataacctcag
    16861 aacagatgat ggatgcctat tccagcgtcg gcatgccaat taactatccg cactggtcat
    16921 tcggtaaaaa gtttatcgag actgaacggc tgtataagca cggtcagcaa gggctggcct
    16981 atgaaatcgt cattaactct aacccgtgta tcgcttactt gatggaagag aacaccatta
    17041 ctatgcaggc gctggtgatg gcacacgcct gctatggaca taactctttc tttaaaaaca
    17101 attgcttatt ccgcagttgg accgacgcca gttcgattgt cgattatctg atttttgccc
    17161 gtaaatatat taccgagtgc gaagaacgtt atggcgttga tgaagtagaa cggcttctgg
    17221 actcgtgcca cgcgctgatg aactacggcg tggaccggta caaacgcccg caaaaaatct
    17281 cgctgcaaga agagaaagcc cggcagaaaa gtcgcgaaga gtatctacaa agtcaggtca
    17341 atatgctctg gcgtaccctg ccgaagcgcg aggaagagaa aacggttgct gaagcgcgcc
    17401 gctatccgtc cgaaccacaa gaaaacctgc tctattttat ggagaaaaat gcgccactgc
    17461 tggaatcatg gcagcgtgaa atcctgcgta ttgtgcgtaa ggtgagccag tatttttatc
    17521 cgcaaaaaca gactcaggtg atgaacgaag gctgtgcgac cttctggcac tataccatcc
    17581 ttaaccatct gtatgatgaa gggaaagtaa cggaacgttt tatgctggag tttttgcaca
    17641 gccacaccaa tgtggtcttc cagccccccc tataacagcc cgtggtacag cggcatcaac
    17701 ccgtatgccc tcgggttcgc catgttccag gatattaaac ggatttgtca gtcgccaacg
    17761 gaagaagaca aatactggtt cccggatatc gccggttccg actggctgga aacgctgcat
    17821 ttcgcgatgc gtgatttcaa agatgagagt tttatcagcc agtttctgtc accgaaagtg
    17881 atgcgtgatt tccgcttctt caccgtgctg gatgatgatc ggcataatta tctggagatt
    17941 tccgctattc ataatgaaga aggttatcgg gagatccgta accggttatc gtcgcaatat
    18001 aacttaagta atctggagcc gaatattcag atctggaacg tggatttgcg cggcgaccgt
    18061 tcgctgacgc tgcgttatat tccacataat cgcgcaccgc tggatcgggg gcgcaaagaa
    18121 gtcctgaagc atgtgcatcg cctgtgggga tttgatgtga tgctcgaaca gcaaaacgaa
    18181 gacggcagca tcgagttgct ggaacgttgc ccgccaagaa tgggaaatct gtaaaaacaa
    18241 caaaaaaccc ctcgtttgag ggggttgctc tttaaacgga agggattatc gcccctgaat
    18301 ggctaaatca cccggcagat ttttctgcat ccggtgccaa atctcgccac tctcatgccc
    18361 atagcgacgc actgtttcgt acacctgatc gtgcgcgcct tcactgcaca acgccgacag
    18421 tttgtggtag aagcccagcg ccagactgcg cgcttccgga ttggcgaaat agtgacgacc
    18481 aatgcgcgta tacagccctt tcatcccgtt aaggatcaga ccgtaaatcg ggttgccgga
    18541 agcaaacgcc aggccgcgga atatgttgta atccagctcg gcaaaggcat cggcgtgatc
    18601 ggccacttca ttagcggtag ccagcacttc ctgcgcttta tcgggatgct gacgaaacgc
    18661 ggtgcgaata aaaatagtgg aaatattggt acgcaccgac agcaaattat caataagctg
    18721 cggcacactt tcgtgatcca gtcgcgccag tgtttcaagg atatttaaac cggaagtttc
    18781 ccagaaatta ttcaccttcg tcggcttgcc atgttgaatg gtcaaccagc catctcgtgc
    18841 cagacgctgt aacacttcac gtaacgtagt acgcgttacg ccaattaatt ctgaaagttc
    18901 acgttctgcg ggcaaaatag tcccgggagg gaagcggtta ttccagatac tttcaataat
    18961 gtactcttcc gcgaaacccg ccgggctttg cgccttaatg accatagtga gatttccata
    19021 acacagcaaa acaaagttgg actcatcata ccagagggct aactacgctg atagcagaca
    19081 aaaagaagaa aaagggatca aggcttcatt ttccgcatct ctttcccctt gactatcaag
    19141 acttgcctca ctcctgcctg accgttaagc tttgcgttta aacataaaaa catcattgat
    19201 tatttgtaag gtaagggaaa ccatcatgga gatctcctgg ggccgcgcgc tatggcgcaa
    19261 ttttttgggc cagtcccccg actggtacaa actcgccctc atcattttct taatcgtaaa
    19321 cccgttaatt ttcctcatca gccctttcgt cgcgggctgg ttgctggtag cggaatttat
    19381 tttcactctg gcgatggccc tgaaatgcta cccgctactc cccggtggtc tgttggctat
    19441 cgaagcggta ttcatcggca tgaccagcgc ggaacacgtc cgtgaagagg tggcggcaaa
    19501 tcttgaagtc ttgctgttat tgatgtttat ggtggcgggt atctatttta tgaaacagct
    19561 gttgctgttc atatttaccc gtttgctgtt aagcattcgc tccaaaatgc tgctgtcgct
    19621 ctctttttgc gtggcggcag cgttcctctc cgcgttcctc gatgccttaa ccgtcgtggc
    19681 ggtggtgatc agcgttgcag tcggttttta tggtatttat catcgcgtag cctcttcccg
    19741 taccgaagac accgacctgc aagacgatag tcatatcgac aagcattaca acgtggttct
    19801 ggaacagttc cgtggctttc tgcgtagcct gatgatgcat gccggtgtcg gcaccgcatt
    19861 aggcggcgta atgaccatgg tgggcgaacc acagaacctg atcatcgcta aagcggctgg
    19921 ctggcatttt ggcgatttct tcctgcgcat gtcgccggtg accgttccgg ttctgatttg
    19981 tggcctgtta acctgcctgc tggtagagaa gctgcgttgg tttggctacg gtgaaacgct
    20041 gccggagaaa gtccgcgaag tgttgcaaca gtttgacgat caaagccgcc accagcgtac
    20101 ccgtcaggat aaaatccgtc tgattgtcca ggcgattatt ggcgtctggc tggtgactgc
    20161 gctggcgttg catctggcgg aagttggctt gattggtttg tcagtcatta ttctggcaac
    20221 atcattgacc ggtgtcaccg atgaacatgc tatcggtaaa gccttcaccg aatctctgcc
    20281 attcaccgca ctgttgacgg tgtttttctc ggtagtcgcg gtgattatcg accaacaact
    20341 gttttcgccg attattcagt ttgtgttgca ggcatctgaa catgctcagc tgtcgctgtt
    20401 ctatattttc aacggtctgc tgtcatccat ttcggataac gtcttcgtgg ggacgattta
    20461 tatcaacgaa gcgaaagcgg caatggaaag tggcgctatc acgttgaagc aatacgagct
    20521 gctggcggtc gccattaata ccggtaccaa tctgccctct gtcgctacgc cgaacggtca
    20581 ggctgcgttc ctgttcctgc tgacctctgc actcgcgcca ttgattcgcc tctcttatgg
    20641 ccgcatggtg tggatggccc tgccttacac cctcgtactg acactcgtcg gcttgctctg
    20701 cgtcgagttt acgcttgccc ctgtaaccga atggtttatg caaatgggct ggatagcaac
    20761 gctttgataa caacttaccg ggcatataaa tgcccggttt gccttttcgc ccgataattg
    20821 tccaattgcg tcctattttt gctgcctcct ggtggcgcag cgaatgaatt ggtttaaact
    20881 gcgcactcta tgcatattgc agggaaatga ttatgttgcg atttttgaac caatgttcac
    20941 aaggccgggg cgcgtggctg ttgatggcgt ttactgctct ggcactggaa ctgacggcgc
    21001 tgtggttcca gcatgtgatg ttactgaaac cttgcgtgct ctgtatttat gaacgctgcg
    21061 cgttattcgg cgttctgggt gctgcgctga ttggcgcgat cgccccgaaa actccgctgc
    21121 gttatgtagc gatggttatc tggttgtata gtgcgttccg cggtgtgcag ttaacttacg
    21181 agcacaccat gcttcagctc tatccttcgc catttgccac ctgtgatttt atggttcgtt
    21241 tcccggaatg gctgccgctg gataaatggg tgccgcaagt gtttgtcgcc tctggcgatt
    21301 gcgccgagcg tcagtgggat tttttaggta tggaaatgcc gcagtggctg ctcggtattt
    21361 ttatcgctta cctgattgtc gcggtgctgg tggtgatttc ccagccgttt aaagcgaaaa
    21421 aacgtgatct gttcggtcgc taatccattc ggcgctcctg cgggagcgct tttttcctgc
    21481 cgctatattt atttgacccg cagtaaatca gaacttcgcg ttgtataacg tggtgaaagc
    21541 atagctcgct tcatctgcca ttgttgttgg atcccctgcc cggcaaaata gagtgttcct
    21601 ctgccctctt tagcattcag agtatccatt accgccatca attgctcact cccggggcgc
    21661 ggtgcgttgt catcgaataa attgagctgc gcgactccct gactgaagaa atcccccagc
    21721 atcacgcccg ctttttggta gcgatggccc gcttgccaga tggcatccag agatcgcgta
    21781 gcagcgttaa tgatatccct gctgtcctga gtgggcgtca gcagttttac cgacgcgcta
    21841 ttgccgtaat aaggttcatt gagcgcaaat ggtgacgtct taataaatgt ggagataaac
    21901 cgacaatatt gatgctcact gcgaagtttt tccgccgccc gggcagcgta actacaaatg
    21961 gcctgccgca tcgacgtata atccgtgatg cgttcaccaa acgagcggga acagataatt
    22021 tcctgcttcg tcggtgcaaa ctcttccagt tgcaaacagg gttcgccgcg cagttcacgc
    22081 accgttcttt cgagcatgac attaaaatgt ttacggataa accggatatc tgtatccgcc
    22141 aaatcgagaa cggttttgat ccccatcgcg tccagttttt tgctgatccg ccgtccaatc
    22201 ccccagacgt catccacggg gagagcagac attaatttac gctggcgttc cagatttgat
    22261 aaatccacca ccccacccgt ctgccgctgc catttttttg ccgcatgatt ggcaagctta
    22321 gccaacgttt tggtctgggc gatccccaca ccaacagtaa gatgggtacg ttgtagcacc
    22381 gttgcgcgaa tttctctgcc aaaatcagtc agatcgcgac aattacgcac acctgtcaga
    22441 tcgcagaatg cttcatcaat actgtaaatc tcgacgcggg gcgatagttc ttccagcgtc
    22501 gacatcaccc gattgctcat gtctgcgtaa agctcataat tgctgctaaa gcaaaccaca
    22561 ccacagcgac gaaacagatc tttttgtttg aaccagggat cgcccatttt aacgcccagc
    22621 gcctttgcct cagcgtttcg ggcgataacg caaccgtcat tattcgatag cacaaccacc
    22681 ggtttacccc ataaatcagg gcgaaacacc gtctcacagc tggcataaaa cgcgtttaca
    22741 tcacagaggg caaacatcag cgcatcgcct taacgacgtg gatcaccaca ccaaagacat
    22801 ccagcgtatc ttcgctactg atggtaatgg gcgagtacgc actgttcatg ggaataagct
    22861 gtaccgtcgg gcgtagttgc aattttttca ccgtaaactc gccgtcaaca gcagcgatga
    22921 caatatcacc atggctggcg gtaatagcac tatcgacaat cagtaaatca ccgtcactaa
    22981 ttccaccatc aatcatagaa tcaccacttg ctttgacgaa gtaagtcgcg ctgggatgct
    23041 ggatcaacag ttgattcaga tcgatgcgct gttcaacgta atctgctgcc ggtgaaggaa
    23101 agccacactg aacaagatcg ctaaatagcg gaaaagtcac aatttcgcgg agatccgcag
    23161 gcttgataaa caacataata atctgcctga agttatactg tttttatata cagtggtctg
    23221 ttcttgccag ca
//
LOCUS       DAZXUK010000035        22954 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZXUK010000035 DAZXUK010000000
VERSION     DAZXUK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22954
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.035"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1602)
                     /gene="glnS"
                     /locus_tag="ACH3L8_001148"
     CDS             complement(<1..1602)
                     /gene="glnS"
                     /locus_tag="ACH3L8_001148"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFT4084349.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTA"
     gene            complement(1805..3751)
                     /gene="nagE"
                     /locus_tag="ACH3L8_001149"
     CDS             complement(1805..3751)
                     /gene="nagE"
                     /locus_tag="ACH3L8_001149"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFT4084350.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            4084..4884
                     /gene="nagB"
                     /locus_tag="ACH3L8_001150"
     CDS             4084..4884
                     /gene="nagB"
                     /locus_tag="ACH3L8_001150"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFT4084351.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            4944..6092
                     /gene="nagA"
                     /locus_tag="ACH3L8_001151"
     CDS             4944..6092
                     /gene="nagA"
                     /locus_tag="ACH3L8_001151"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFT4084352.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6101..7321
                     /gene="nagC"
                     /locus_tag="ACH3L8_001152"
                     /gene_synonym="nagR"
     CDS             6101..7321
                     /gene="nagC"
                     /locus_tag="ACH3L8_001152"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFT4084353.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7369..8121
                     /gene="nagD"
                     /locus_tag="ACH3L8_001153"
                     /pseudo
     CDS             7369..8121
                     /gene="nagD"
                     /locus_tag="ACH3L8_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            complement(8172..8270)
                     /locus_tag="ACH3L8_001154"
                     /pseudo
     CDS             complement(8172..8270)
                     /locus_tag="ACH3L8_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8518..10182
                     /gene="asnB"
                     /locus_tag="ACH3L8_001155"
     CDS             8518..10182
                     /gene="asnB"
                     /locus_tag="ACH3L8_001155"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFT4084354.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10561..10637
                     /locus_tag="ACH3L8_001156"
     tRNA            10561..10637
                     /locus_tag="ACH3L8_001156"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10595..10597,aa:Met,seq:cat)
     gene            10646..10730
                     /locus_tag="ACH3L8_001157"
     tRNA            10646..10730
                     /locus_tag="ACH3L8_001157"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10680..10682,aa:Leu,seq:tag)
     gene            10754..10828
                     /locus_tag="ACH3L8_001158"
     tRNA            10754..10828
                     /locus_tag="ACH3L8_001158"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10786..10788,aa:Gln,seq:ttg)
     gene            10863..10937
                     /locus_tag="ACH3L8_001159"
     tRNA            10863..10937
                     /locus_tag="ACH3L8_001159"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10895..10897,aa:Gln,seq:ttg)
     gene            10953..11029
                     /locus_tag="ACH3L8_001160"
     tRNA            10953..11029
                     /locus_tag="ACH3L8_001160"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10987..10989,aa:Met,seq:cat)
     gene            11078..11152
                     /locus_tag="ACH3L8_001161"
     tRNA            11078..11152
                     /locus_tag="ACH3L8_001161"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11110..11112,aa:Gln,seq:ctg)
     gene            11190..11264
                     /locus_tag="ACH3L8_001162"
     tRNA            11190..11264
                     /locus_tag="ACH3L8_001162"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11222..11224,aa:Gln,seq:ctg)
     gene            complement(11418..12593)
                     /gene="ubiF"
                     /locus_tag="ACH3L8_001163"
     CDS             complement(11418..12593)
                     /gene="ubiF"
                     /locus_tag="ACH3L8_001163"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFT4084355.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12739..14163
                     /gene="miaB"
                     /locus_tag="ACH3L8_001164"
     CDS             12739..14163
                     /gene="miaB"
                     /locus_tag="ACH3L8_001164"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFT4084356.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDASHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14316..15356
                     /locus_tag="ACH3L8_001165"
     CDS             14316..15356
                     /locus_tag="ACH3L8_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFT4084357.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15353..15820
                     /gene="ybeY"
                     /locus_tag="ACH3L8_001166"
     CDS             15353..15820
                     /gene="ybeY"
                     /locus_tag="ACH3L8_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFT4084358.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15910..16788
                     /gene="corC"
                     /locus_tag="ACH3L8_001167"
     CDS             15910..16788
                     /gene="corC"
                     /locus_tag="ACH3L8_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFT4084359.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16813..18351
                     /gene="lnt"
                     /locus_tag="ACH3L8_001168"
     CDS             16813..18351
                     /gene="lnt"
                     /locus_tag="ACH3L8_001168"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFT4084360.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18748..19656
                     /gene="gltI"
                     /locus_tag="ACH3L8_001169"
     CDS             18748..19656
                     /gene="gltI"
                     /locus_tag="ACH3L8_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFT4084361.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19826..20566
                     /gene="gltJ"
                     /locus_tag="ACH3L8_001170"
     CDS             19826..20566
                     /gene="gltJ"
                     /locus_tag="ACH3L8_001170"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFT4084362.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20566..21240
                     /gene="gltK"
                     /locus_tag="ACH3L8_001171"
     CDS             20566..21240
                     /gene="gltK"
                     /locus_tag="ACH3L8_001171"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFT4084363.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21240..21965
                     /gene="gltL"
                     /locus_tag="ACH3L8_001172"
     CDS             21240..21965
                     /gene="gltL"
                     /locus_tag="ACH3L8_001172"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFT4084364.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22083..>22954
                     /gene="rihA"
                     /locus_tag="ACH3L8_001173"
     CDS             22083..>22954
                     /gene="rihA"
                     /locus_tag="ACH3L8_001173"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFT4084365.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATV"
BASE COUNT         5577 a         5990 c         5919 g         5468 t
ORIGIN      
        1 ggccgtggaa tggcggctgt cgaggcagaa gtaaccttca cgctcaaact ggaatgcttt
       61 acccgcaacc gcatctttca gcgacggttc agcaaagccc tgtttgatca ccagcgattc
      121 cgggttaatc accgacagga aatcatccgc agcacctggg ttaggcacgc tgaacagacg
      181 atcatacaaa cggatttcaa ccggcagcgc atgtgccgcg ctcacccagt gaataacacc
      241 tttgacttta cgaccatctg ccggatcttt gcttaaggtg tcggcgtcat aagtacagaa
      301 gatggtggtg atattacctt cggcatcttt ctccacgcgt tcagccttaa tgacgtaagc
      361 attacgcaga cgcacttctt tacccagcac cagacgtttg tactgcttgt tagcttcttc
      421 gcggaaatcg gcgcgatcga tccaaatttc accgctaaac ggcacctgac ggctccccat
      481 ttccggtttg ttcggatggt tcggcatggt gaccatttcg ccttcgccct gatagttttc
      541 gataaccagt ttcaccggat cgataaccgc cattgcgcgc ggcgcatttt cgttgagatc
      601 ttcacggatg caggattcca gcgacgccat ctcaatggtg ttgtcctgct tggtcacgcc
      661 gatgcgtttg cagaactcac gaatagaagc cgcagtgtaa ccacgacgac gcagaccgga
      721 aatggtcggc atacgcgggt catcccagcc ttcaacgtgc ttgtcggtca ccagcaggtt
      781 cagcttacgc ttggacatca cagtgtattc cagattcagg cgcgagaatt cgtactggcg
      841 cggatgaaca ggaatggtga tgttatccag tacccagtcg tacagacgac ggttgtcctg
      901 gaactcaagc gtacacagag agtgcgtaat accttccagc gcatcgctga tgcaatgggt
      961 gaagtcgtac atcgggtaga tgcaccactt gttgccagtc tggtggtgtt cagcaaattt
     1021 aatgcggtac agcaccggat cgcgcattac gataaatggc gacgccatgt cgattttcgc
     1081 acgcaggcag gctttacctt cttcaaaacc accggcacgc attttttcga acagcgccaa
     1141 gttctcttca acgctgcggt cgcggtacgg gctgttttta cccggctgcg tcagggtacc
     1201 gcggtattcg cggatctgtt ccggtgtcag ttcatcaacg tacgccaggc ctttattgat
     1261 cagttcgatc gcataggcgt ggagctgatc aaaataatcg gaggagtaac ggacgttacc
     1321 agaccagtga aaacctaacc actctacgtc gtttttgatc gaatcgacat actcgatatc
     1381 ttcttttacc ggattagtgt cgtcgaaacg caggttgcac tggcctttat agtcctgggc
     1441 gatcccgaag ttcaggcaga tagatttcgc atggccaata tgcagatagc cattcggctc
     1501 cggcgggaaa cgggtgtgta ctgtgatgtg cttaccactg gccagatctt catcgatgat
     1561 ctgacggata aagttagtcg ggcgggcttc tgcctcactc atcgtggatt cctcaaagcg
     1621 taaacaacgt ataacggcgt atgatcttat aagcgggaca ggctgacaac tgttagtttt
     1681 ttaaaattga taatctaaca aggattatgg ggatgattga tgaaaaaaaa agcggcagag
     1741 aagatctcca ccgcttgtca ttgttggatg cgacgctgcc gtgtcgcatc aggcataaag
     1801 cagattactt tttgatttca tacagcggtg tttgacccgc cacaacatgg ccctgagctt
     1861 taatgagcaa gccactgaaa tcgtcgatat tgctgcaaac caccgggcta atcatcgagc
     1921 gggcgttagc gttcaggtaa tccagatcca tttccagaat cggttgccct gcgcttacct
     1981 gcgcaccctc ttccaccaga cgtttaaagc ctttaccttc cagcgctacg gtgtcgatac
     2041 ccatatggac gacgatctcc gcgccttttt cggtttccag gcagaacgcg tggttggtgt
     2101 tgaagatttt cacgattgtc cctgcggctg gtgatacgac gattttatct gtcggtttca
     2161 ccgccacacc gtcacccacc gctttgctgg cgaatgcttc gtcaggaacc tgatccagtg
     2221 ccacgacatc accggtaatc ggcgatacca gctccgcgat agataccgcg tttggtacag
     2281 cctgcggttt tgctacaggc gcggcagttg ccggagttgc ttcagctgac gcagcggcta
     2341 ccggaccacg ggcaacgact ttcttcatcg catcgccgat ggattctgct ttcgcgccaa
     2401 caatcacctg aatagtctgt ttgttcagtt tcactacacc agaagcaccc agacgtttac
     2461 acatcgtatc gttgacgcgg gcagagtcag ccacggtcag gcgcagacgg gtaatacagg
     2521 catcaatcgc tttcaggttg tcagtaccgc caaccgcagc aatatagttg gttgccagtt
     2581 gagtcagacc ttcttcagta ttgctgttgg cttcttcagt aacgatctcg tcttctttat
     2641 cttcacgacc cggcgttttc aggttgaaca tgcggataac caaactgaac accacgaagt
     2701 agatagcgaa gaagataacg cccatcacca gcagcatcca gacgttctgg ctggcggcag
     2761 gcaggttata catcaacgcg tagtcgatag cacccgcaga gaaagagaag ccggcatgga
     2821 tacccagcag cgttgccaca aacaggctga taccggtcag cagtgcgtgc aggaggtaca
     2881 gcagcggcgc aaggaacatg aacaggaatt ccagcggctc agtcacaccg gtcaggaacg
     2941 cagtaacagc aacagaaagc agcatcccgc caaccatcgg acgacgctct ttcggtgctg
     3001 cgaagtacat cgccagcgcc gcacccggca gaccgaacat catgatcggg aagaagccgg
     3061 acatgaacat ccccgcggtg ccgtcaccag cgtagaaacg gttgatgtcg ccgtggaaaa
     3121 ccgtacccgc cgcgttggtg aattcaccaa tctggaacca ggcgatggtg ttcagcacct
     3181 gatgcagacc ggttgggatc agcagacggt tgatgaaacc aaagataccg gaacccagcg
     3241 cgcccgcaga aacgatccac tcgccgcctg catggatagc gtgctgtacc ggcggccaga
     3301 cgtaaccaaa aatggccgcc agcaccagac agaagaagcc ggtggcgatc ggcacaaagc
     3361 gtttgccgcc gaagaagctc aggaagtccg gcagtttaat atcggaccaa cggttatagg
     3421 ctgcgccacc aaccagaccg gtaatgatac ccgccagtac acccatgtta atttctgggt
     3481 tgatggtcac catcgctttg gttaacacaa agtaacctac cgcacccgcc agcgccgccg
     3541 cacctgcgct gtctttcgac cagctggatg ccacaccgat ggcgaagatt aatgcgaggt
     3601 tatcaaaaat tgcaccgccc gcctgggcaa taaacgcaac gttaagtaaa tctggctgac
     3661 cgaatcgcag caacagcgcc gccaccggca gcaccgcgat agggagctgt aacgccctac
     3721 cgagtcgctg gaaaaaacct aaaatattca tcttattccc cctacgagaa ccctatttgg
     3781 ctcgtttaaa gccgtatttt tattttgctg caaattttac tgccgatgtt ctgtaatcag
     3841 attgttagat catctgctac agagtgtgtg aaaatttaat tcgcatcgca aattaaacgc
     3901 gtgtcttttg tgagttttgt caccaaatat cgttatcatc actccctttt actggctaaa
     3961 ccagaaaact tattttatca ttcaaaaaat caagtcggat tgacgcctgt ctgcgcaaat
     4021 ccaggttacg cttaaagatg tctaatccgc caacggctta cattttactt attgaggtga
     4081 ataatgagac tgatccccct gactaccgct gaacaggtcg gcaaatgggc tgctcgccat
     4141 atcgtcaatc gtatcaatgc gttcaaacca actgccgatc gtccgtttgt actgggcctg
     4201 ccgactggcg gcacgccgat gaccacctat aaagcgttag tcgaaatgca taaagcaggc
     4261 caggtcagct ttaagcacgt tgtcaccttc aacatggacg aatatgtcgg tctgccgaaa
     4321 gagcatccgg aaagctacta cagctttatg caccgtaatt tcttcgatca cgttgatatt
     4381 ccagcagaaa acatcaacct tctcaacggc aatgccccgg atatcgatgc cgagtgccgc
     4441 cagtatgaag aaaaaatccg ttcttacgga aaaattcatc tgtttatggg cggtgtaggt
     4501 aacgacggtc atattgcatt taacgaaccg gcgtcttctc tggcttctcg tactcgtatc
     4561 aaaaccctga ctcatgacac tcgcgtcgcg aactctcgtt tctttgataa cgatgtcaat
     4621 caggtgccaa aatatgccct gactgtcggt gttggtacac tgctggatgc cgaagaagtg
     4681 atgattatgg tgctgggtag ccagaaagca ctggcgctgc aggccgccgt tgaaggttgc
     4741 gtgaaccata tgtggaccat cagctgtctg caactgcatc cgaaagcgat catggtgtgc
     4801 gatgaacctt ccaccatgga gctgaaagtt aagactttaa gatatttcaa tgaattagaa
     4861 gcagaaaata tcaaaggtct gtaattgtta tccctgccct ggttccttgc tcagggcaat
     4921 attttttaaa atcggggggc agaatgtatg cattaaccca gggccggatc tttaccggcc
     4981 acgaatttct tgatgaccac gcggttgtta tcgctgatgg cctgattaaa agcgtctgtc
     5041 cggtagcgga actgccgcca gagatcgaac aacgttcact gaacggggcc attctctccc
     5101 ccggttttat cgatgtgcag ttaaacggct gcggcggcgt acagtttaac gacaccgctg
     5161 aagcggtcag cgtggaaacg ctggaaatca tgcagaaagc caatgagaaa tcaggctgta
     5221 ctaactatct gccgacgctt atcaccacca gcgatgagct gatgaaacag ggcgtgcgcg
     5281 ttatgcgtga gtacctggca aaacatccga atcaggcgtt aggtctgcat ctggaaggtc
     5341 cgtggctgaa tctggtaaaa aaaggcaccc ataatccgaa ttttgtgcgt aagcctgatg
     5401 ccgcgctggt cgatttcctg tgtgaaaacg ccgacgtcat taccaaagtg accctggcac
     5461 cggaaatggt tcctgcggaa gtcatcagca aactggcaaa tgccgggatt gtggtttctg
     5521 ccggtcactc caacgcgacg ttgaaagaag caaaagccgg tttccgcgcg gggattacct
     5581 ttgccaccca tctgtacaac gcgatgccgt atattaccgg tcgtgaaccg ggcctggcgg
     5641 gcgcgatcct cgacgaagct gacatttatt gcggtattat cgctgatggc ctgcatgttg
     5701 attacgccaa cattcgtaac gctaaacgcc ttaaaggcga caaactgtgt ctggttaccg
     5761 acgccaccgc gccagcaggt gccaacattg aacagttcat ttttgcgggt aaaacaatat
     5821 actaccgtaa cggactttgt gtggatgaga acggtacgtt aagcggttca tccttaacca
     5881 tgattgaagg cgtgcgtaat ctggtcgaac attgcggtat cgcactggat gaagtgctgc
     5941 gtatggcgac gctctatccg gcgcgtgcga ttgacgttga aaaacgtctc ggcacgctcg
     6001 ccgcaggtaa agtagccaac ctgaccgcat tcacacctga ttttaaaatc accaagacca
     6061 tcgttaacgg taacgaggtc gtaactcaat aagagaaagt atgacaccag gcggacaagc
     6121 tcagataggt aatgttgatc tcgtaaaaca gcttaacagc gcggcggttt atcgcctgat
     6181 tgaccagtac gggccaatct cgcggattca gattgccgag caaagccagc ttgcccccgc
     6241 cagcgtaacc aaaattacgc gtcagcttat cgaacgcggg ctgatcaaag aagttgatca
     6301 gcaggcctcc accgggggcc gccgcgctat ctccatcgtc accgaaaccc gcaatttcca
     6361 cgcaatcggc gtacggcttg gtcgtcatga cgccaccatc actctgtttg atctcagcag
     6421 caaagtgctg gcagaagaac attacccgct gccggaacgt acccagcaga cgctggaaca
     6481 tgccctgctg aatgccattg ctcagtttat tgatagctac cagcgcaagc tgcgcgagct
     6541 aatcgccatt tcggtgatcc tgccagggct tgttgacccg gacagcggca aaattcatta
     6601 catgccgcat attcaggtag aaaactgggg gctggtagaa gcactggaag aacgttttaa
     6661 agtgacctgt ttcgttggtc acgatatccg tagtctggcg ctggcggagc actacttcgg
     6721 tgcaagtcag gattgcgaag actccattct ggtgcgtgtc catcgcggaa ccggggccgg
     6781 gattatctct aacgggcgca tttttattgg ccgcaacggt aacgtcggtg aaattggcca
     6841 tattcaggtc gaaccgctgg gtgaacgctg ccactgcggc aactttggct gcctggaaac
     6901 tatcgccgcc aacgctgcca ttgaacaacg ggtgttgaat ctgttaaagc agggctacca
     6961 gagccgtgtg ccactggacg actgcaccat caaaactatc tgcaaagccg cgaacaaagg
     7021 cgatagcctg gcctcggaag tgattgagta tgtcggtcgt catctgggta aaaccatcgc
     7081 cattgctatc aacttattta atccgcaaaa aattgttatt gctggtgaaa tcaccgaagc
     7141 cgataaagtg ctgctccctg ctattgaaag ctgcattaat acccaagcgc tgaaggcgtt
     7201 tcgtactaat ctgccggtgg tacgttctga gctggatcac cgctcggcaa tcggcgcttt
     7261 tgcgctggta aaacgcgcca tgctcaacgg tattttgctc cagcatttgc tggaaaatta
     7321 atgtgctttt atagtggcgc ttattgttgt caatattctg ggtagtccat gaccattaaa
     7381 aatgtaattt gcgatatcga cggcgtgctg atgcacgata acgtcgccgt accgggtgca
     7441 gcggaatttt tgcacgggat tatggataaa ggcctgccgc tggtgttgct gaccaactat
     7501 ccttcgcaga ctgggcaaga tctggcgaac cgctttgcca ctgccggtgt cgatgtgccg
     7561 gatagcgtgt tttatacctc tgcgatggcg accgccgatt tcctgcgtcg ctaggaaggc
     7621 aagaaagcgt atgtggtggg cgaaggcgca ctgattcatg aactgtacaa agccggtttc
     7681 actattaccg atgtgaaccc tgatttcgtg attgttggcg aaacacgttc ctacaactgg
     7741 gacatgattc ataaagcagc ctatttcgtc gctaacggtg cacgctttat cgccaccaac
     7801 ccggacaccc acgggcgcgg tttttatccc gcttgtggcg cgttgtgtgc agggattgag
     7861 aaaatctccg ggcgcaaacc gttctatgtt ggtaagccca gcccgtggat catccgcgcg
     7921 gcattaaaca aaatgcaggc gcattcggaa gaaacagtga ttgtcggcga taacctgcgt
     7981 accgatattc tggcgggctt ccaggccggt ctggagacga ttctggtgct ttctggtgtt
     8041 tcatcgctcg acgatatcga cagtatgccg ttccgcccaa gctggattta cccgtcagtc
     8101 gctgaaatcg acgttatctg aataccaccc acgtctgcgg acgtggttgg ctctgatgac
     8161 aaacgccaaa ctgcctgatg cgctacgctt atcaggccta cgcagctcct gcaatatatt
     8221 gaattttcat gcttttgtag gccggataag gcgttcacgc cgcatccggc atgaacaaat
     8281 cgcactttat cagcaatttg gcccccgtaa aatataacca ttcaataaaa cccgctaaaa
     8341 attatcgatt catcaataaa tacatcctca caccagaccc tttttcacca atcgacaatc
     8401 accattacgt tttttatttt tgcactgcca aatagcttgc gcacagtgct gctttgcggc
     8461 atttttttaa acaagcaaac acaacaagca acaaatacca ggttaacgga gaaggttatg
     8521 tgttcaattt ttggcgtatt cgatatcaaa acagacgcag ttgagctgcg taagaaagca
     8581 ctcgagctgt cacgcctgat gcgtcatcgt ggcccggact ggtccggtat ttatgccagc
     8641 gataacgcca ttctcgctca cgaacgtctg tcaattgttg acgttaacgc gggggcgcaa
     8701 cctctctaca accaacaaaa aaaccacgta ctggcggtaa acggtgaaat ctacaaccac
     8761 caggcattgc gcgctgaata tggcgatcgt tatcagttcc agacagggtc tgactgtgaa
     8821 gtgatcctcg cgctgtatca ggaaaaaggg ccggaatttc tcgacgactt gcagggcatg
     8881 tttgcctttg ccctgtacga cagcgaaaaa gatgcctacc tgattggtcg cgaccatctg
     8941 gggatcatcc cactgtatat ggggtatgac gaacacggtc agctgtatgt ggcctcagaa
     9001 atgaaatcgc tggtgccagt ttgccgcacg attaaagagt tcccggcggg gagctatttg
     9061 tggagtcagg acggcgaaat ccgttcttat tatcatcgcg actggttcga ctttgatgca
     9121 gtgaaagata acgtgaccga caaaaacgag ctgcgtcagg cactggaaga ttccgttaaa
     9181 agccatctga tgtctgatgt gccttacggt gtgctacttt ctggtggtct ggattcctca
     9241 attatttccg ctatcaccaa gaaatacgca gcccgtcgcg tggaagatca ggaacgctct
     9301 gaagcctggt ggccgcagtt acactccttt gctgtaggtc tgccgggttc accggatctt
     9361 aaagcagccc aggaagtggc aaaccatctg ggcacggtgc atcacgaaat tcacttcact
     9421 gtacaggaag gtctggatgc catccgcgac gtgatttacc acatcgaaac ttatgatgtg
     9481 accactattc gcgcttcaac accgatgtat ttaatgtcgc gtaagatcaa ggcgatgggc
     9541 attaaaatgg tgctgtccgg tgaaggttct gatgaagtgt ttggcggtta tctttacttc
     9601 cataaagcgc ccaacgttaa agaactgcat gaagagacgg tgcgtaaact gctggccttg
     9661 catatgtatg actgcgcgcg cgccaacaaa gcgatgtcag cctggggcgt ggaagcacgc
     9721 gttccgttcc tcgacaaaaa attccttgat gtggcgatgc gcattaaccc gcaggataaa
     9781 atgtgcggta acggcaaaat ggaaaaacac atcctgcgtg aatgttttga gtcatacctg
     9841 cccgcaagcg tggcctggcg gcagaaagag cagttctccg atggcgtcgg ttacagttgg
     9901 atcgacaccc tgaaagaagt ggcggcgcag caggtttctg atcagcaact ggaaactgcc
     9961 cgcttccgct tcccgtacaa cacgccgacc tcaaaagaag cgtatctgta ccgcgagatc
    10021 tttgaagaac tgttcccact tccgagtgcc gctgagtgcg tgccgggcgg tccttccgtc
    10081 gcgtgttctt cagctaaagc gatcgaatgg gatgaagcgt tcaaaaaaat ggacgatccg
    10141 tctggtcgtg cggttggcgt tcaccagtcg gcgtataagt aagaaatgcg gtactgcccc
    10201 ggagaaatcc ggggctgttt tagttattgc gctgcttgtt gattctgata tgcagtaagg
    10261 aaatgttccg ggtttgccac atcactgttt gacaactcat cactgtaggc cagtatttca
    10321 tagattaatt tcccaccact attgttgaat tccgaatttc atcctgtacg acaaaagaat
    10381 ggctgtaggg tggggaaaaa cacaaagtaa acaataattg acgaatatag cgccacgctg
    10441 ttcgcaacct aaccaaacag tcactttcga gcaattttcc ttgaaaaaga ggttgacgct
    10501 gcaaggctct atacgcataa tgcgccccgc aacgccgata aggtatcgcg aaaaaaagat
    10561 ggctacgtag ctcagttggt tagagcacat cactcataat gatggggtca caggttcgaa
    10621 tcccgtcgta gccaccatct tttttgcggg agtggcgaaa ttggtagacg caccagattt
    10681 aggttctggc gccgcaaggt gtgcgagttc aagtctcgcc tcccgcacca ttcaccagaa
    10741 agcgttgtac ggatggggta tcgccaagcg gtaaggcacc ggtttttgat accggcattc
    10801 cctggttcga atccaggtac cccagccatc ttcttcgagt aagcggttca ccgcccggtt
    10861 attggggtat cgccaagcgg taaggcaccg gtttttgata ccggcattcc ctggttcgaa
    10921 tccaggtacc ccagccatcg aagaaataat ctggctacgt agctcagttg gttagagcac
    10981 atcactcata atgatggggt cacaggttcg aatcccgtcg tagccaccaa attctgaatg
    11041 tgtcgaatac gttcggcaaa ttcaaaaacc aatttgttgg ggtatcgcca agcggtaagg
    11101 caccggattc tgattccggc attccgaggt tcgaatcctc gtaccccagc caatttattc
    11161 aagacgctta catcgtaagt gcacccagtt ggggtatcgc caagcggtaa ggcaccggat
    11221 tctgattccg gcattccgag gttcgaatcc tcgtacccca gccacattaa aaaagctcgc
    11281 ttcggcgagc tttttgcttt tctgcgttca ttcaatgtcg aatgcgatgt tgacacgtct
    11341 tatccttcaa tgtcggatgc gacgctgccg cgtcttatcc gacctacggc tggcacgcat
    11401 ccggcaatgt tgtaaggcta caaccctaac gcatatttca gcgcctgacg tttcaacacg
    11461 ccagcacgct ctgccgccat taacccgaga ttacgcacaa aacgcagcgg cggcagatta
    11521 ttactgaatc cggcataaaa cagatccata ccactttgca taatgaagtt atccgccatg
    11581 cgccgcatct ggtaacgctt gaggacagga taactggccc acgcttcacc gtagctgcgg
    11641 gcattcacca gaacatcaat cagggcatcg acatcacgat aaccaagatt caccccctgc
    11701 cccgccagcg gatggatggt atgcgcggca tcgcccacca gcgcaagccc cggctgcaca
    11761 tactgcaacg catgtcggcg cgtcagcgga aacgcaccag cggcaagcgg tgtaacgtaa
    11821 cccagacgcg acgggaaatg cttcgcgatt tccgcctgga gttgcgccat attcatattc
    11881 tgcaactggc gaatacgcgt cggagagtca taccacacca gcgatgccca gttatcaaac
    11941 aacggcagaa acgcacgcgg tccgtccggg gtaaattgct gccaggtgct gtcgccggga
    12001 tcgttctcac actgtacgct aatcaacatg cacgactgcg catactgcca ggcatgaacg
    12061 ccaattcccg ccatctgccg cacctgagaa tttgcgccgt cggcaccaat caccagtttc
    12121 gcgcggatcg tttcaccgcc cttcagttcc agctcctgaa gatcattatc tcgatgtaat
    12181 gcaatcagcg aggttggcac acgtaacgtt actttcggat gcgcttccag cgcctgccac
    12241 aacgcctgtt gcaggactgt gttttccacc atgtagccaa gcaacggcaa ctttaattcg
    12301 gctgcgtcaa ataccacatg tgccgtttcc cactcccagg tttccagtct gcggtaagga
    12361 tggcaacgca tagcctgtac tgcatcccag acccctaacc ctttaagcaa tgataccgaa
    12421 gccgcgctga tcgccgagat ccgcacgtcc ggttggctgt cagcgacaaa cggtgcaggt
    12481 tctgcgtgct cgatcaccgt taccgaaaat ccgtgctgtg ccagccccag cgccagtgcg
    12541 ccgccgacca ttcctccgcc gacaatggca atttccgttg gttgatttgt catggtcaat
    12601 catcctgtta gcaatatgct ttaagtttac aggattttta cccccaaggg ctgataacgc
    12661 tcatactggt cacaacggca gcaaagcatt acactatgcg ccctgcattc ctggctacta
    12721 tttcgcaaga gcaagtcgat gaccaaaaaa ctccatatta aaacctgggg ctgtcagatg
    12781 aacgagtacg attcatcgaa gatggccgat ctgctggatg cctcccacgg ctatcaactg
    12841 accgacgtgg cggaagaagc ggatgtgcta ctgctgaaca cctgctcaat ccgcgagaag
    12901 gctcaggaaa aagtcttcca tcagttgggt cgctggaaac tgttaaaaga gaagaatcca
    12961 gacctgatta tcggcgtcgg tggctgcgtg gcatcgcaag aaggcgagca cattcgccag
    13021 cgcgcccact atgtcgatat tatttttggg ccgcaaacgc tgcaccgtct gccggagatg
    13081 atcaactccg tgcgcggcga ccgcagcccg gttgtagata tcagcttccc ggaaatcgag
    13141 aagtttgacc gtctgccgga accgcgcgcc gaagggccga ccgcgtttgt ctccatcatg
    13201 gaaggctgca ataaatattg cacctactgc gtggtgcctt acacccgtgg tgaagaggta
    13261 agccgtccgt ctgacgatat tctgtttgag attgcccagc ttgcggctca gggcgtgcgt
    13321 gaagtcaacc tgctcggtca gaatgtgaat gcctggcgtg gtgagaacta cgacggcacc
    13381 accggatcgt ttgccgatct gttgcgtctg gttgctgcga tcgacgggat cgatcgtatt
    13441 cgctttacca ccagccatcc gatcgaattc accgacgata tcatcgaagt gtaccgcgac
    13501 acgcctgagc tggtgagctt cctgcatctg ccggtacaga gcggttccga tcgcattctg
    13561 aacctgatgg gacgtaccca tacggcgctg gagtacaaag cgatcatccg taaactgcgt
    13621 gcggcgcgtc cggatattca gatcagttct gacttcattg ttggcttccc tggcgaaacc
    13681 accgaagact tcgagaaaac gatgaagctg attgccgacg tcaatttcga catgagctac
    13741 agctttatct tctctgcacg tccaggaacg cctgccgccg atatggttga tgatgttccg
    13801 gaagaagaga agaagcagcg tctgtatatt ctgcaagaac gtatcaatca gcaagcgatg
    13861 gcatggagcc gtcggatgct cggcaccacc cagcgtattc tggtagaagg gacttcgcgt
    13921 aagagcatca tggagctttc cgggcgtacc gaaaataacc gcgtggtcaa cttcgagggc
    13981 acaccggata tgatcggtaa attcgtcgat gtagaaatta ccgacgtcta cccgaactct
    14041 ctgcgcggta aagtggtgcg aactgaagat gaaatgggtc tgcgcatggc agaaacaccg
    14101 gagtcggtga ttgcccgcac ccgcaaagaa aacgaccttg gcgtgggtta ttatcagccg
    14161 taattctcag gcctgccgtt ccggtgggcc ttgttttttc tctctttatc cccatcttta
    14221 tcgcaatgct tgcgcctttt tcctgtggcg tgaataattt ccttataggc cggtgaatgt
    14281 tcagcttccg gcggcataca gataagagga acggtttgaa catagacact cgcgaaatca
    14341 ccctggagcc agcagacaat gcgcgtctgt tgagcctgtg cggcccgttt gatgacaaca
    14401 tcaagcagct cgaacgccgt ctcggcatcg agatcaatcg ccgcgataac cactttaaac
    14461 tgaccggccg tccgatttgc gtcaccgctg cggcagacat tctgcgtagc ctgtatgtcg
    14521 atactgcccc gatgcgcggt cagattcagg atatcgaacc ggaacagatc caccttgcga
    14581 ttaaagaagc gcgggtactg gagcaaagcg cggagagcgt gccggagtac ggcaaagcgg
    14641 tcaatatcaa aactaaacgc ggcgtaatta agccgcgcac gccaaaccag gcgcagtaca
    14701 tcgccaatat tctcgaccat gacattacct tcggcgttgg cccggcgggt acgggtaaaa
    14761 cctacctggc agtggctgcg gcagttgatg ccctggagcg tcaggaaatt cgccgtattc
    14821 tgctgactcg tccggcggtc gaagccggtg agaaactggg cttcctgcct ggcgatttaa
    14881 gccagaaagt agacccgtat ctgcgcccac tgtacgacgc gctgtttgaa atgctgggct
    14941 ttgagaaagt cgagaaactg attgagcgca acgttattga agtcgcgccg ctggcctata
    15001 tgcgtggtcg tacgctgaac gacgcgttta tcattctcga tgagagccag aacactacca
    15061 tcgaacagat gaagatgttc ctgacccgta tcggttttaa ctcaaaagcg gttatcaccg
    15121 gcgacgtcac acagatcgac ctgccgcgta atactaaatc aggcttacgt catgccattg
    15181 aagtgctggc cgatgtcgaa gagatcagct ttaacttctt ccacagcgaa gacgtggttc
    15241 gtcacccggt ggtggcgcgt atcgttaacg cttatgaagc ctgggaagaa gcagaacaaa
    15301 aacgtaaagc ggcgctggca gcagaacgca agcgcgaaga acaggaacaa aaatgagtca
    15361 ggtgatcctc gatttacaac tggcatgtga agataattcc gggttaccgg aagagagcca
    15421 gtttcaaaca tggctgaatg cggtgatccc gcagtttcag gaagagtcgg aagtgactat
    15481 tcgcgtggtc gataccgccg aaagccacag tctgaatctg acctatcgcg gtaaggataa
    15541 gccgaccaac gtgctctcct tcccgtttga agtgccgcct ggcatggaga tgtcgctact
    15601 gggcgatctg gttatctgcc gtcaggtggt tgagaaggaa gctcaggagc aaggcaaacc
    15661 actggaggcg cactgggcgc atatggtggt gcacggcagt ctgcatttgt taggttacga
    15721 tcacattgaa gatgacgaag cagaagaaat ggaagccctc gaaacagaga ttatgcttgc
    15781 tctgggctat gaggatccgt acattgccga gaaagaataa tttgccaggc atttgactgg
    15841 cagacgtccc cgccgttggt gattaacgac ggcgttttga ttaactaaca tgagaaccca
    15901 tacgacgcca tgagcgacga caattcacac agtagtgaca cgataagcaa caagaaggga
    15961 tttttctccc tgttactcag ccaacttttc cacggtgaac cgaaaaaccg tgacgaactg
    16021 ctggcgctga tccgtgattc cgggcagaac gaccttatcg acgaagatac gcgcgatatg
    16081 ctcgaagggg tgatggacat cgcagaccaa cgcgttcgcg acatcatgat cccccgctcc
    16141 cagatgatta ccctgaaacg caaccagacg ctggacgaat gccttgatgt catcatcgag
    16201 tccgcccact cacgtttccc ggtgattagc gaagacaaag atcacattga agggattctg
    16261 atggcgaaag acttgctgcc gtttatgcgc agcgatgctg aagccttcag catggacaaa
    16321 gtgttacgtc aggcggttgt cgttcctgaa agtaagcgcg tagaccggat gctgaaagag
    16381 tttcgctctc agcgttacca catggcgatc gttattgacg aattcggtgg ggtttccggt
    16441 ctggtgacca ttgaagacat cctggaactg attgttggtg agattgaaga cgagtatgac
    16501 gaagaagatg atatcgactt ccgtcagctg agtcgtcata cctggaccgt gcgcgcactg
    16561 gcttccattg aagacttcaa cgaagcgttc ggcacccact ttagcgatga agaagtcgac
    16621 actatcggtg gtctggtgat gcaggcgttt gggcatcttc cggcgcgtgg cgaaactatc
    16681 gacatcgacg gttaccagtt caaagtggcg atggccgata gtcggcgtat tattcaggtt
    16741 catgtcaaaa tcccggatga ctcaccccag ccaaagctgg atgaataaaa ccgaaactgg
    16801 atagataact acatggattt tgcctcatta attgaacgcc agcgcattcg cctgctgctg
    16861 gcgttattat tcggtgcctg cggaacgctg gccttctctc cttacgacgt ctggcctgcg
    16921 gcgattattt cgctgatggg gcttcaggcg ttgaccttta accgtcgccc actccagtct
    16981 gccgctattg gcttttgctg gggatttggc ctctttggca gcggtattaa ctgggtctat
    17041 gtcagcatcg cgacctttgg cgggatgcct ggcccggtta acatcttcct ggtggtgctg
    17101 ctggcggcgt atttgtcgct gtataccgga ctgtttgctg gcgtgctgtc gcgtctgtgg
    17161 ccgaaaacca cctggctgcg cgtggcgatt gccacccctg ctctctggca agtgaccgag
    17221 tttctgcgcg gttgggtgct gactggcttc ccgtggttac agttcggcta tagccagatt
    17281 gatggcccgt taaaagggct ggcaccgcta atgggcgtgg aagccattaa cttcctgctg
    17341 atgatggtta gtggcctgct ggcactggcg ttggtcaaac gcaactggcg tccgctggtg
    17401 gtggccgtcg tgctgtttgc cctacccttc ccgctgcgtt acatccaatg gtttaccccg
    17461 caaccggaga aaaccattca ggtttcgatg gttcagggcg atattccgca atcgctgaaa
    17521 tgggacgaag gccagcttct taatacgcta aagatttact acaacgcaac ggcaccgctg
    17581 atgggcaaat catcgttgat tatctggccg gagtcggcga taaccgatct ggaaattaat
    17641 cagcaaccgt tcctcaaagc actggacggt gagttgcgtg ataaaggtag ctcgctggta
    17701 accgggattg tcgacgcgcg tctcaataag cagaaccgct acgataccta caacaccatc
    17761 atcacgctgg gtaaaggtgc accgtacagc tacgaatcag ccgatcgcta taacaaaaac
    17821 catctggtgc cgtttggcga gtttgtcccg ctggagtcga ttctgcgtcc gttagcaccg
    17881 ttctttgatc tgccgatgtc gtcgttcagc cgtgggccat atatccagcc gccgctgtcg
    17941 gcaaatggta ttgagcttac tgcggctatt tgctacgaga ttattctcgc cgagcaagtg
    18001 cgcgataact tccgcccgga taccgactac ctgctgacta tctccaacga tgcgtggttt
    18061 ggtaaatcta ttggtccatg gcaacacttc cagatggcgc gaatgcgtgc gctggagctg
    18121 gcgcgcccac tgttgcgcag caccaacaac ggcattacgg cggtgattgg cccgcagggt
    18181 gagattcagg cgatgatccc gcagttcgcc cgcgaggtgt taaccactaa cgtgacgccg
    18241 accaccggac tcacaccata cgcacgtacc ggcaactggc cgctgtgggt gctgacggca
    18301 ctgtttggtt ttgctgctgt gttgatgagt ctgcgtcagc gacgtaaata atcccttctt
    18361 atcgtgccgg aatacatccc atatttcggc acgctcaccg gatgcggcat cagtgcatta
    18421 tccggataac aatattctcc tatccaactt tggcacatct attgctttgt tatacaaggc
    18481 aaagcttgaa ccagcatcag tacaacccgg ttgcaccaca gcaatccacc ggtagcacca
    18541 aaagagtgca ttttaagaat tttgcttcag aatggtgcgg tgcacctcgc aaaaacaaac
    18601 aataaaacat caatatcttt acatttagcc aaactaaatg ttacctaaca ggcacaacac
    18661 tgcacaataa agttgcagac gataacaaca caaacactca caacgggtat ccatgcgttc
    18721 ttaacgcaga agataaaggt gttggatatg caattacgta aacctgccac agcaatcctc
    18781 gccctggcgc tttccgcagg actggcacag gcagatgacg ccgccccggc agcaggcagt
    18841 acgctggaca aaatcgccaa aaacggtgtg attgtcgtcg gtcaccgtga atcttcagta
    18901 cctttctctt attacgacaa tcagcaaaaa gtggtgggtt actcgcagga ttactccaac
    18961 gccattgttg aagcagtgaa aaagaaactc aacaaaccgg acttgcaggt aaaactgatt
    19021 ccgattacct cacaaaatcg tattccactg ctgcaaaacg gcactttcga ttttgaatgt
    19081 ggttctacca ccaacaacgt cgaacgccaa aaacaggcgg ctttctctga cactattttc
    19141 gtggtcggta cgcgcctgtt gaccaaaaag ggcggcgata tcaaagattt tgccgacctg
    19201 aaaggcaaag ccgtagtcgt cacttccggc actacctctg aagttttgct caacaaactg
    19261 aatgaagagc aaaaaatgaa tatgcgcatc atcagcgcca aagatcacgg tgactctttc
    19321 cgcaccctgg aaagcggtcg tgccgttgcc tttatgatgg atgacgctct gctggccggt
    19381 gaacgtgcga aagcgaagaa accagacaac tgggatatcg tcggcaagcc gcagtctcag
    19441 gaagcctacg gttgcatgtt gcgtaaagac gatccgcagt tcaaaaagct gatggatgac
    19501 actatcgctc aggtgcagac ctccggtgaa gcggaaaaat ggtttgataa atggttcaaa
    19561 aatccaattc cgccgaaaaa cctgaacatg aatttcgaac tgtcagacga aatgaaagca
    19621 ctgttcaaag aaccgaatga caaggcactg aactaattac aagaaccagg ggcggaaatt
    19681 ccagccctct cgattgttac gtagcacgga cagactatac gcctgatggt cgttccccat
    19741 cgggcctgaa aaccgcaata cgctgggtaa caatcttcga gggtagcagt taacgctgct
    19801 accctctttt ttctggagta gatttatgtc tatagactgg aactggggta tttttttaca
    19861 acaagccccg ttcggcaata ccacctatct tggttgggtc tggagtggtt ttcaggtgac
    19921 gatcgctttg tcgatctgcg cctggattat cgctttcctc gtcggttcat ttttcggcat
    19981 tttacgtacc gttccaaacc gttttctctc cggtctgggt acgttgtatg tcgaactgtt
    20041 ccgcaacgtg ccgttgattg tgcaattctt tacctggtat ctggtgatcc cggagctgct
    20101 gccggagaaa atcggcatgt ggtttaaggc cgagctggat cccaatattc agtttttcct
    20161 ttcatccatg ctctgcctgg ggctgtttac cgccgcccgt gtttgcgaac aggtacgcgc
    20221 cgctattcag tcgctaccgc gtgggcagaa gaatgccgcg ctggcgatgg gcctgacgct
    20281 accgcagact tatcgatacg tactgctgcc taatgcttat cgcgttatcg tcccgccgat
    20341 gacttccgag atgatgaacc tggtgaaaaa ctccgccatc gcctcgacca tcggtctggt
    20401 ggatatggcg gcgcaggcgg gtaaattgct cgactactcg gctcacgcct gggagtcgtt
    20461 taccgccatc acgcttgcgt atgtgttgat taacgctttt atcatgctgg tgatgacgct
    20521 ggttgaacgt aaagttcgcc tgcccggcaa catggggggc aaataatgta cgagtttgac
    20581 tggagttcca ttgttccttc cctgccatat ctgctcgacg ggctggtgat caccctgaaa
    20641 atcaccgtca cggcagtcgt gattggtatt ttgtggggta ccatgctggc ggtgatgcgc
    20701 ttgtccagct tcgcgcctgt ttcgtggttt gccaaagcct acgttaacgt attccgctcg
    20761 attcctttag tcatggtttt gctgtggttt tacttgattg tgccgggatt tctgcaaaat
    20821 gtgctgggat tatcgccaaa aaatgatatt cgtttaattt cggcgatggt ggcgttttcg
    20881 atgtttgaag cggcctacta ttcagagatt atccgtgccg gtattcaaag tatttcccgt
    20941 ggtcagtcga gcgccgcgct ggcgttgggg atgactcact ggcagtcgat gaaactgatt
    21001 attctgccgc aggctttccg cgcgatggtg ccgctgctgc tcactcaggg catcgtactg
    21061 ttccaggata cctcgctggt gtatgtgtta tctctggccg atttcttccg taccgcctca
    21121 accattggtg agcgtgatgg tacgcaggtc gagatgatcc tgtttgccgg atttgtttat
    21181 ttcgttatta gccttagcgc gtcgttgttg gtcagctact tgaaaagaag gacagcataa
    21241 tgattaccct gaaaaatgtt tcaaaatggt atggtcactt tcaggtgctg accgactgct
    21301 caaccgaagt gaaaaaaggc gaagtggtgg tggtttgcgg cccgtctggt tccggcaaat
    21361 caacgctgat taaaaccgtc aacggcctcg aaccagtgca gcaaggtgaa atcaccgtcg
    21421 atggtatcgt ggttaacgac aagaaaaccg atctggcaaa gctgcgctcc cgcgtcggga
    21481 tggtattcca gcatttcgag ctgttccctc atctgtcgat tatcgaaaac ctgaccctgg
    21541 cgcaggtgaa agtgcttaaa cgcgataaag cgcccgctcg tgaaaaagca ttgaaattgc
    21601 tggagcgcgt gggactttcc gcacatgcca ataaatttcc ggcgcagctt tccggcggtc
    21661 agcagcagcg cgtggcaatc gctcgcgcat tgtgtatggc tcctattgcg atgctgttcg
    21721 acgaaccgac atcggcgctg gatccggaga tgatcaacga agtgctggac gtgatggtgg
    21781 aactggcgaa cgaaggaatg accatgatgg tggtgaccca cgaaatgggc tttgcccgca
    21841 aagtggcgaa tcgggtgatc tttatggacg agggtaaaat tgtcgaagac tcgccgaaag
    21901 acgctttctt cgatgatccg aaatcggacc gcgcaaaaga cttcctcgcg aaaatcctgc
    21961 attaatcact ctggcgcaca tacccaattg cgcgccatct cacgctttat cgtttcgccc
    22021 ctcgtatctg ttacatcctg gcgttaccct tgtcgcaaag aagcacacaa caaggagcaa
    22081 caatggcact gccaattctg ttagattgcg acccaggtca tgatgacgct atcgcaatag
    22141 ttctcgcact cgcctcacca gagcttgatg tcaaagcaat tacgtcttcc gccggaaacc
    22201 agacaccaga aaaaacctta cgcaatgttc tgcgtatgct gaccttgctt aatcgcaccg
    22261 atattccggt ggcaggcggc gcggtaaaac cgttaatgcg tgagttgatt atcgcggaca
    22321 atgtgcacgg cgaaagcggt ctcgacggcc cggcattacc ggaaccgacg ttcgcaccgc
    22381 aaaactgtac ggcggtggag ctgatggcga aaacgctgcg cgaaagtgcg gaacctgtca
    22441 cgattgtttc taccggaccg caaactaacg ttgccttgct gctcaatagc catccggaac
    22501 tgcatagcaa aattgcccgt atcgtgatta tgggcggcgc aatggggctt ggtaactgga
    22561 cgcctgcggc tgaatttaat atttacgttg acccggaagc ggcagaaatt gtcttccagt
    22621 cagggatccc ggtggtgatg gccggtctgg atgttactca taaagcgcaa atccacgttg
    22681 aagacaccga gcgtttccgc gcgattggca accctgtttc aaccattgtt gccgaactgc
    22741 tggatttctt cctcgaatat cataaagacg aaaaatgggg ctttgtcggc gcaccgctgc
    22801 atgacccatg caccatcgcc tggctgttga aacccgagtt gtttaccact gttgagcgct
    22861 gggttggcgt ggaaacacag gggaaatata cccagggtat gacggttgtt gattattatt
    22921 atctgactgg caataaaccg aatgccactg taat
//
LOCUS       DAZXUK010000036        21886 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZXUK010000036 DAZXUK010000000
VERSION     DAZXUK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21886
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.036"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..2026
                     /gene="metE"
                     /locus_tag="ACH3L8_001174"
     CDS             <1..2026
                     /gene="metE"
                     /locus_tag="ACH3L8_001174"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFT4084366.1"
                     /translation="VPPRHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNY
                     HYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPVLLGPVTYLWLGKVKGEQFDRL
                     SLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLL
                     LTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVELHKRLPSDWLLSAGLINGRNVW
                     RADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHE
                     LALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAITAQDSQRANVYEVR
                     AEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVE
                     QERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDISRP
                     APITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVAD
                     LEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYC
                     EFNDIMDSIAALDADVITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEW
                     IEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLRRG"
     gene            complement(2065..2880)
                     /locus_tag="ACH3L8_001175"
     CDS             complement(2065..2880)
                     /locus_tag="ACH3L8_001175"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFT4084367.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            3142..3903
                     /gene="udp"
                     /locus_tag="ACH3L8_001176"
     CDS             3142..3903
                     /gene="udp"
                     /locus_tag="ACH3L8_001176"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFT4084368.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            4044..5471
                     /gene="rmuC"
                     /locus_tag="ACH3L8_001177"
     CDS             4044..5471
                     /gene="rmuC"
                     /locus_tag="ACH3L8_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFT4084369.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            5566..6321
                     /gene="ubiE"
                     /locus_tag="ACH3L8_001178"
     CDS             5566..6321
                     /gene="ubiE"
                     /locus_tag="ACH3L8_001178"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFT4084370.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            6335..6940
                     /gene="ubiJ"
                     /locus_tag="ACH3L8_001179"
     CDS             6335..6940
                     /gene="ubiJ"
                     /locus_tag="ACH3L8_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFT4084371.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            6937..8577
                     /gene="ubiB"
                     /locus_tag="ACH3L8_001180"
     CDS             6937..8577
                     /gene="ubiB"
                     /locus_tag="ACH3L8_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFT4084372.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            8656..8925
                     /gene="tatA"
                     /locus_tag="ACH3L8_001181"
     CDS             8656..8925
                     /gene="tatA"
                     /locus_tag="ACH3L8_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFT4084373.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            8929..9444
                     /gene="tatB"
                     /locus_tag="ACH3L8_001182"
     CDS             8929..9444
                     /gene="tatB"
                     /locus_tag="ACH3L8_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFT4084374.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            9447..10223
                     /gene="tatC"
                     /locus_tag="ACH3L8_001183"
     CDS             9447..10223
                     /gene="tatC"
                     /locus_tag="ACH3L8_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFT4084375.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            10265..11047
                     /gene="tatD"
                     /locus_tag="ACH3L8_001184"
     CDS             10265..11047
                     /gene="tatD"
                     /locus_tag="ACH3L8_001184"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFT4084376.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11044..11532)
                     /gene="rfaH"
                     /locus_tag="ACH3L8_001185"
     CDS             complement(11044..11532)
                     /gene="rfaH"
                     /locus_tag="ACH3L8_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFT4084377.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            11699..13192
                     /gene="ubiD"
                     /locus_tag="ACH3L8_001186"
     CDS             11699..13192
                     /gene="ubiD"
                     /locus_tag="ACH3L8_001186"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFT4084378.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            13238..13939
                     /gene="fre"
                     /locus_tag="ACH3L8_001187"
     CDS             13238..13939
                     /gene="fre"
                     /locus_tag="ACH3L8_001187"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFT4084379.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(14131..15294)
                     /gene="fadA"
                     /locus_tag="ACH3L8_001188"
     CDS             complement(14131..15294)
                     /gene="fadA"
                     /locus_tag="ACH3L8_001188"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFT4084380.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(15304..17493)
                     /gene="fadB"
                     /locus_tag="ACH3L8_001189"
     CDS             complement(15304..17493)
                     /gene="fadB"
                     /locus_tag="ACH3L8_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFT4084381.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            17683..19014
                     /gene="pepQ"
                     /locus_tag="ACH3L8_001190"
     CDS             17683..19014
                     /gene="pepQ"
                     /locus_tag="ACH3L8_001190"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFT4084382.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            19014..19628
                     /locus_tag="ACH3L8_001191"
     CDS             19014..19628
                     /locus_tag="ACH3L8_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFT4084383.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            19667..21118
                     /gene="trkH"
                     /locus_tag="ACH3L8_001192"
     CDS             19667..21118
                     /gene="trkH"
                     /locus_tag="ACH3L8_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFT4084384.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            21130..21675
                     /gene="hemG"
                     /locus_tag="ACH3L8_001193"
     CDS             21130..21675
                     /gene="hemG"
                     /locus_tag="ACH3L8_001193"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFT4084385.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         5064 a         5579 c         6048 g         5195 t
ORIGIN      
        1 tgttccgcca cgtcatcaga acaaagacgg ttcggtagat atcgacaccc tgttccgtat
       61 tggtcgtgga cgtgcgccga ctggcgaacc tgcggcggca gcggaaatga ccaaatggtt
      121 taacaccaac tatcactaca tggtgccgga gttcgttaaa ggccaacagt tcaaactgac
      181 ttggacgcag ctgctggagg aagtggacga ggcgctggcg ctgagccaca acgttaagcc
      241 tgtgctgctg gggccggtta cttacctgtg gctggggaaa gtgaaaggtg aacagtttga
      301 ccgccttagc ctgctgaacg acattctgcc ggtttatcag caagtgctgg cagaactggc
      361 gaaacgcggc atcgagtggg tacagattga tgaacccgcg ctggtactgg aactaccaca
      421 ggcgtggctg gacgcataca aacccgctta cgacgcgctc cagggacagg tgaaactgct
      481 gctgaccacc tattttgaag gcgtaacgcc aaatctcgac acgattactg cgctgcctgt
      541 tcaggggctg catgtggacc ttgtacatgg taaagataac gttgttgaac tgcacaagcg
      601 cctgccttct gactggctgc tgtctgcggg tctgatcaat ggtcgtaacg tctggcgcgc
      661 cgatcttact gagaaatatg cgcaaattaa ggacattgtc ggcaaacgtg atttgtgggt
      721 ggcatcttcc tgctcgttgc tgcacagccc catcgacctg agcgtggaaa cgcgtcttga
      781 tgcagaagtg aaaagctggt ttgccttcgc cctacaaaaa tgccatgaac tggcactgct
      841 gcgcgatgcg ctgaacagtg gtgacacggc agctctggca gagtggagcg ccccaattca
      901 ggcgcgtcgt cactctactc gtgtacataa tccggcggta gaaaagcgtc tggcggcgat
      961 caccgcccag gacagccagc gtgcgaatgt ctatgaagtg cgtgctgaag cccagcgtgc
     1021 gcgttttaaa ctgccagcgt ggccgaccac cacgattggt tccttcccgc aaaccacgga
     1081 aattcgtacc ctgcgtctgg atttcaaaaa gggcaatctc gacgccaaca actaccgcac
     1141 gggcattgcg gaacatatca agcaggccat tgttgagcag gaacgtttgg gactggatgt
     1201 gctggtacat ggcgaggccg agcgtaatga catggtggaa tactttggcg agcacctcga
     1261 cggatttgtc tttacgcaaa acggttgggt acagagctac ggttcccgct gcgtgaagcc
     1321 accgattgtc attggtgaca ttagccgccc ggcaccgatt accgtggagt gggcgaagta
     1381 tgcgcaatcg ctgaccgaca aaccggtgaa agggatgctg acggggccgg tgaccatact
     1441 ctgctggtcg ttcccgcgtg aagatgtcag ccgtgaaacc atcgccaaac agattgcgct
     1501 ggcgctgcgt gatgaagtgg ccgatctgga agccgctgga attggcatca tccagattga
     1561 cgaaccggcg ctgcgcgaag gtttaccgct gcgtcgtagc gactgggatg cgtatctcca
     1621 gtggggcgta gaggccttcc gtatcaacgc cgccgtggcg aaagatgaca cacaaatcca
     1681 cactcacatg tgttattgcg agttcaacga catcatggat tcgattgcgg cgctggacgc
     1741 agacgtcatc accatcgaaa cctcgcgttc cgacatggag ttgctggagt cgtttgaaga
     1801 gtttgattat ccaaatgaaa tcggtcctgg cgtctatgac attcactcgc caaacgtacc
     1861 gagcgtggaa tggattgaag ccttgctgaa gaaagcggca aaacgcattc cggcagagcg
     1921 cctgtgggtc aacccggact gtggcctgaa aacgcgcggc tggccagaaa cccgcgcggc
     1981 actggcgaac atggtgcagg cggcgcagaa tttgcgtcgg gggtaaatcc aaaccgggtg
     2041 gtaataccac ccggtctttt ctcattacag cgacttcttc ccaccatact gcttaaacca
     2101 ttccagcata cgctgccagc catctttcgc agattcggca tgatagctcg ggcgataatc
     2161 agcgttgaat gcatgcccgg catccgggta cacgataatc tctgctttcg cattagcagc
     2221 ccgcagcgcc tggcgcatcg tttcaacgct ctcctgcgga atgctgttat cctgaccacc
     2281 atataagccg agaaccggcg cgttaagatc ggttgcgata tcaacaggtt gtttcggtga
     2341 attcagcgac ttgtcgcccg tcagtttgcc gtaccacgcc actgcggctt ttagctgtgg
     2401 attatgcgcg gcatacagcc aggtgatacg tccaccccag cagaatccgg tgatcattaa
     2461 acgatgaaca tcgccgccgt tgcgggaagc ccaactggcg acatgatcga gatcggccag
     2521 cacctgcgag tcaggcactt ttgctaccag accgctaagc aacgtgggga tatcggcaaa
     2581 atcattcgga tcgccttcgc ggaagtaaag ttcaggtgcg atagccagat acccctccag
     2641 cgccagacgg cgacaaatgt cgcggatatg ttcatgcacg ccaaaaattt cctgcactac
     2701 aatgaccact ggcagtgggc catcgctttg ctttggtctg gcatggtaag caggcatgtt
     2761 atccccttgt gaagggatag aggtgaagcc cgccacaatc gcgtcgtccg gggtctgaac
     2821 gatggtcgaa gcgagaggcg atgcagcagg tgcaaatcca gattgttgtg ttgttgccat
     2881 ggtattctcc gtacccttat aaaaatgttg cgcaatgtta actatagtca gcatgcaaca
     2941 aatcacattg cctgaatcgg ctcatctttt atgcagtcct gcagaatgaa gagtgattta
     3001 tgtgatatga atcacttttg gtgggtaaat ttatgcaacg catttgcgtc atggtgatga
     3061 gtatcacgaa aaaatgttaa acccttcggt aaagtgtctt tttgcttctt ctgactaaac
     3121 cgattcacag aggagttgta tatgtccaag tctgatgttt ttcatctcgg cctcactaaa
     3181 aacgatttac aaggggctac gcttgccatc gtccctggcg acccggatcg tgtggaaaag
     3241 atcgccgcgc tgatggataa gccggttaag ctggcatctc accgcgaatt cactacctgg
     3301 cgtgcagagc tggatggtaa acctgttatc gtctgctcta ccggtatcgg cggcccgtct
     3361 acctctattg ctgttgaaga gctggcacag ctgggcattc gcaccttcct gcgtatcggt
     3421 acaacgggcg ctattcagcc gcatattaat gtgggtgatg tcctggttac cacggcgtct
     3481 gtccgtctgg atggcgcgag cctgcacttc gcaccgctgg aattcccggc tgtcgctgat
     3541 ttcgaatgta cgactgcgct ggttgaagct gcgaaatcta ttggcgcgac aactcacgtt
     3601 ggcgtgacag tttcttctga taccttctac ccaggtcagg aacgttacga tacttactct
     3661 ggtcgcgtag ttcgtcactt taaaggttct atggaagagt ggcaggcgat gggcgtaatg
     3721 aactatgaaa tggaatctgc aactctgctg accatgtgtg caagtcaggg cctgcgtgcc
     3781 ggtatggtag cgggtgttat cgttaaccgc acccagcaag agatcccgaa tgctgagacg
     3841 atgaaacaaa ccgaaagcca tgcggtgaaa atcgtggtgg aagcggcgcg tcgtctgctg
     3901 taattctctt ctcctgtctg aaggccgacg cgttcggcct tttgtatttt tgcgtagcgc
     3961 ctcgcaggaa atgcctttcc aactggacgt ttgtacagca caattctatt ttgtgcgggt
     4021 aagttgttgc gtcaggaggc gttgtggatt tctcaatcat ggtttacgca gttattgcgt
     4081 tggtgggtgt ggcaattggc tggctgtttg ccagttatca acatgcgcag caaaaagccg
     4141 agcaattagc tgaacgtgaa gagatggtcg cggagttaag cgcggcaaaa caacaaatta
     4201 cccaaagcga gcactggcgt gcagagtgtg aattgctcaa taacgaagtg cgcagcctgc
     4261 aaagtattaa cacctctctg gaggccgatc tgcgtgaagt aaccacgcgg atggaagccg
     4321 cacagcaaca tgctgacgat aaaattcgcc agatgattaa cagcgagcag cgcctcagtg
     4381 agcagtttga aaacctcgcc aaccgtattt ttgagcacag caatcgccgg gttgatgagc
     4441 aaaaccgtca gagtctgaac agcctgttgt cgccgctacg tgaacaactg gacggtttcc
     4501 gccgtcaggt tcaggacagc ttcggtaaag aagcacaaga acgccatacc ctgacccacg
     4561 aaattcgcaa tctccagcaa ctcaacgcgc aaatggccca ggaagcgatc aacctgacgc
     4621 gcgcgctgaa aggcgacaat aaaacccagg gcaactgggg cgaggtagta ttgacgcggg
     4681 tgctggaggc ttccggtctg cgtgaagggt atgaatatga aacccaggtc agcatcgaaa
     4741 atgacgcccg ctcgcggatg cagccggatg tcatcgtgcg gctgccgcag ggaaaagatg
     4801 tggtgatcga cgccaaaatg acgctggtcg cctatgaacg ctattttaac gccgaagacg
     4861 actacacccg cgaaagcgcg ctacaggaac atatcgcgtc ggtgcgtaac catatccgtt
     4921 tgctgggacg caaagattat caacagctgc cggggctgcg aactctggat tacgtgctga
     4981 tgtttattcc cgttgaaccc gcttttttac tggcgcttga ccgccagccg gagctgatca
     5041 ccgaagcgtt gaaaaacaat atcatgctgg ttagcccgac tacgctgctg gtggcgttgc
     5101 gtactattgc caacctgtgg cgttatgagc atcaaagccg taacgcccag caaatcgccg
     5161 atcgtgccag caagctgtac gacaagatgc gtttgttcat cgatgacatg tccgcgattg
     5221 gtcaaagtct cgacaaagcg caggataact accgccaggc aatgaaaaaa ctctcttcag
     5281 gtcgcggaaa tgtgctggcg caggcagaag cgtttcgcgg tttaggagta gaaattaaac
     5341 gcgagattaa tccggatttg gctgaacagg cggtgagcca ggatgaagag tatcgacttc
     5401 ggtcggttcc ggagcagccg aatgatgaag cttatcaacg cgatgatgaa tataatcagc
     5461 agtcgcgcta gcccattagg ggtagttaag ccgggtagaa atctagggca tcgacgccca
     5521 atctgttaca cttctggaac aattttttga tgagcaggca ttgagatggt ggataagtca
     5581 caagaaacga cgcactttgg ttttcagacc gtcgcgaagg aacaaaaagc ggatatggtc
     5641 gcccacgttt tccattccgt ggcatcaaaa tacgatgtca tgaatgattt gatgtcattt
     5701 ggtattcatc gtttgtggaa gcgattcacg attgattgca gcggcgtacg ccgtgggcag
     5761 accgtgctgg atctggctgg tggcaccggc gacctgacag cgaaattctc ccgcctggtc
     5821 ggagaaactg gcaaagtggt ccttgctgat atcaatgaat ccatgctcaa aatgggacgc
     5881 gagaagctgc gtaatatcgg tgtgattggc aacgttgagt atgttcaggc gaacgctgag
     5941 gcgctgccgt tcccggataa cacctttgat tgcatcacca tttcgtttgg tctgcgtaac
     6001 gtcaccgaca aagataaagc actgcgttca atgtatcgcg tgctgaaacc cggcggccgc
     6061 ctgctggtgc ttgagttctc gaagccaatt atcgagccgc tgagcaaagc ctatgatgca
     6121 tactccttcc atgtgctgcc gcgtattggc tcactggtcg cgaacgacgc cgacagctac
     6181 cgttatctgg cagaatccat ccgtatgcat cccgatcagg ataccctgaa agccatgatg
     6241 caggatgccg gattcgaaag tgtcgactac tacaatctga cggcaggggt tgtggcgctg
     6301 catcgtggtt ataagttctg acaggagacc ggaaatgcct tttaaacctt tagtgacggc
     6361 aggaattgaa agtctgctca acaccttcct gtatcgctca cccgcgctga aaacagcccg
     6421 ctcgcgtctg ctgggtaaag tattgcgcgt ggaggtaaaa ggcttttcga cgtcattgat
     6481 tctggtgttc agcgaacgcc aggttgatgt actgggcgaa tgggcaggcg atgctgactg
     6541 caccgttatc gcctacgcca gtgtgttgcc gaaacttcgc gatcgccagc agcttaccgc
     6601 actgattcgc agtggtgagc tggaagtgca gggcgatatt caggtggtgc aaaacttcgt
     6661 tgcgctggca gatctggcag agttcgaccc tgcggaactg ctggcccctt ataccggtga
     6721 tatcgccgct gaaggaatca gcaaagccat gcgcggaggc gcaaagttcc tgcatcacgg
     6781 cattaagcgc cagcaacgtt atgtggcgga agccattact gaagagtggc gtatggcacc
     6841 cggtccgctt gaagtggcct ggtttgcgga agagacggct gccgtcgagc gtgctgttga
     6901 tgccctgacc aaacggctgg aaaaactgga ggctaaatga cgccaggtga agtacggcgc
     6961 ctatatttca tcattcgcac ttttttaagc tacggacttg atgaactgat ccccaaaatg
     7021 cgtatcaccc tgccgctacg gctatggcga tactcattat tctggatgcc aaatcggcat
     7081 aaagacaaac ttttaggtga gcgactacga ctggccctgc aagaactggg gccggtttgg
     7141 atcaagttcg ggcaaatgtt atcaacccgc cgcgatcttt ttccaccgca tattgccgat
     7201 cagctggcgt tattgcagga caaagttgct ccgtttgatg gcaagctggc gaagcagcag
     7261 attgaagctg caatgggcgg cttgccggta gaagcgtggt ttgacgattt tgaaatcaag
     7321 ccgctggctt ctgcttctat cgcccaggtt cataccgcgc gattgaaatc gaatggtaaa
     7381 gaggtggtga ttaaagtcat ccgcccggat attttgccgg tcattaaagc ggatctgaaa
     7441 cttatctacc gtctggctcg ctgggtgccg cgtttgctgc cggatggtcg ccgtctgcgc
     7501 ccaaccgaag tggtgcgcga gtacgaaaag accttgattg atgaactgaa tttgctgcgg
     7561 gaatctgcca acgccattca gcttcggcgc aattttgaag acagcccgat gctctacatc
     7621 ccggaagttt accctgacta ttgtagtgaa gggatgatgg tgatggagcg catttacggc
     7681 attccggtgt ctgatgttgc ggcgctggag aaaaacggca ctaacatgaa attgctggcg
     7741 gaacgcggcg tgcaggtgtt cttcactcag gtctttcgcg acagcttttt ccatgccgat
     7801 atgcaccctg gcaacatctt cgtaagctat gaacacccgg aaaacccgaa atatatcggc
     7861 attgattgcg ggattgttgg ctcgctaaac aaagaagata aacgctatct ggcagaaaac
     7921 tttatcgcct tctttaatcg cgactatcgc aaagtggcag agctacacgt cgattctggc
     7981 tgggtgccac cagataccaa cgttgaagag ttcgaatttg ccattcgtac ggtctgtgaa
     8041 cctatctttg agaaaccgct ggccgaaatt tcgtttggac atgtactgtt aaatctgttt
     8101 aatacggcgc gtcgcttcaa tatggaagtg cagccgcaac tggtgttact ccagaaaacc
     8161 ctgctctatg tcgaaggggt aggacgccag ctttatccgc agctcgattt atggaaaaca
     8221 gcgaagcctt tcctggagtc gtggattaaa gatcaggtcg gtattcctgc cctggtgaga
     8281 gcatttaaag aaaaagcgcc gttctgggtc gaaaaaatgc cagaactgcc tgaactggtt
     8341 tacgacagtt tgcgccaggg caagtattta cagcacagtg ttgataagat tgcccgcgag
     8401 cttcagtcaa atcatgtacg tcagggacaa tcgcgttatt ttctcggaat tggcgctacg
     8461 ttagtattaa gtggcacatt cttgttggtc agccgacctg agtgggggct gatgcccggc
     8521 tggttaatgg caggtggtct gatcgcctgg tttgtcggtt ggcgcaaaac acgctgattt
     8581 tttcatcgct caaggcgggc cgtgtaacgt ataatgcggc tttgtttaat catcatctac
     8641 cacagaggaa catgtatggg tggtatcagt atttggcagt tattgattat tgccgtcatc
     8701 gttgtactgc tttttggcac caaaaagctc ggctccatcg gttccgatct tggtgcgtcg
     8761 atcaaaggct ttaaaaaagc aatgagcgat gatgaaccaa agcaggataa aaccagtcag
     8821 gatgctgatt ttactgcgaa aactatcgcc gataagcagg cggatacgaa tcaggaacag
     8881 gctaaaacag aagacgcgaa gcgccacgat aaagagcagg tgtaatccgt gtttgatatc
     8941 ggttttagcg aactgctatt ggtgttcatc atcggcctcg tcgttctggg gccgcaacga
     9001 ctgcctgtgg cggtaaaaac ggtagcgggc tggattcgcg cgttgcgttc actggcgaca
     9061 acggtgcaga acgaactgac ccaggagtta aaactccagg agtttcagga cagtctgaaa
     9121 aaggttgaaa aggcgagcct cactaacctg acgcccgaac tgaaagcgtc gatggatgaa
     9181 ctacgccagg ccgcggagtc gatgaagcgt tcctacgttg caaacgatcc tgaaaaggcg
     9241 agcgatgaag cgcacaccat ccataacccg gtggtgaaag ataatgaagc tgcgcatgag
     9301 ggcgtaacgc ctgccgctgc acaaacgcag gccagttcgc cggaacagaa gccagaaacc
     9361 acgccagagc cggtggtaaa acctgctgcg gacgctgaac cgaaaaccgc tgcaccttcc
     9421 ccttcgtcga gtgataaacc gtaaacatgt ctgtagaaga tactcaaccg cttatcacgc
     9481 atctgattga gctgcgtaag cgtctgctga actgcattat cgcggtgatc gtgatattcc
     9541 tgtgtctggt ctatttcgcc aatgacatct atcacttggt atccgcgcca ttgatcaagc
     9601 agttgccgca aggttcaacg atgatcgcca ccgacgtggc ctcgccgttc tttacgccga
     9661 tcaagctgac ctttatggtg tcgctgattc tgtcagcgcc ggtgattctc tatcaggtgt
     9721 gggcatttat cgccccagcg ctgtataagc atgaacgtcg cctggtggtg ccgctgctgg
     9781 tttccagctc tctgctgttt tatatcggca tggcattcgc ctactttgtg gtctttccgc
     9841 tggcatttgg cttccttgcc aataccgcgc cggaaggggt gcaggtatcc accgacatcg
     9901 ccagctattt aagcttcgtt atggcgctgt ttatggcgtt tggtgtctcc tttgaagtgc
     9961 cggtggcaat tgtgctgctg tgctggatgg ggattacctc gtcagaagac ttacgcaaaa
    10021 aacgcccgta tgtgctggtt ggtgcattcg ttgtcgggat gttgctgacg ccgccggatg
    10081 tcttctcgca aacgctattg gcgatcccga tgtactgtct gtttgaaatc ggtgtcttct
    10141 tctcacgctt ttacgttggt aaagggcgaa atcgggaaga ggaaaacgac gctgaagcag
    10201 aaagcgaaaa aactgaagaa taaattcaac cgcccgtcag ggcggttgtc atatggagta
    10261 caggatgttt gatatcggcg ttaatttgac cagttcgcaa tttgcgaaag accgtgatga
    10321 tgttgtagcg cgcgcttttg acgcgggagt taatgggcta ctcatcaccg gcactaacct
    10381 gcgtgaaagc cagcaggcgc aaaagctggc gcgtcagtat tcgtcctgtt ggtcaacggc
    10441 gggcgtacat cctcacgaca gcagccagtg gcaagctgcg actgaagaag cgattattga
    10501 gctggccgcg cagccagaag tggtggcgat tggtgaatgt ggtctcgact ttaaccgtaa
    10561 cttttcgacg ccggaagagc aggaacgcgc ttttgttgct cagctacgca ttgccgcaga
    10621 attaaacatg ccggtattta tgcactgtcg cgatgcccac gagcggttta tgacattgct
    10681 ggagccgtgg ctggataaac tgcctggtgc ggttcttcat tgctttaccg gcacacgcga
    10741 agagatgcag gcgtgcgtgg cgcgtggaat ttatatcggc attaccggtt gggtttgcga
    10801 tgaacgacgc gggctggagc tgcgggaatt gttgccgttg attccggcgg agaaattgct
    10861 gatcgaaaca gatgcgccgt atctgctccc gcgcgatctc acgccaaagc catcatcccg
    10921 gcgcaacgag ccagcccatc tgccccatat tttgcaacgt attgcgcact ggcgtggaga
    10981 agatgccgca tggctggctg ccaccacgga tgccaatgtc aaaacactgt ttgggattgc
    11041 gttttagagt ttgcggaact cggtgttctt cacactgtgc ttaatctctt tattaataag
    11101 attaagcaat agcatggagc gagcctcacc atcgggttcg gtgaaaatgg cctgaaagcc
    11161 ttcgaacgcg ccttcggtaa taatcacctt atcacccgga taaggggggg ccggatcgac
    11221 aatgtctttc ggtttatata ccgatagctg atgaattacc gccgatggga ctatcgctgg
    11281 cgacgcgcca aagcgcacaa agtggctgac gccacgcgta gcgctgatgg tcgtggtatg
    11341 aatcacttct ggatcaaatt ccacaaacag gtagttaggg aataacggtt cactgactgc
    11401 ggtacgttta ccccgtacga ttttttccag ggtgatcatc ggagcgaggc aattaaccgc
    11461 ctgtctctca aggtgttcct gggcacgttg aagttgcccg cgcttgcagt acagtaaata
    11521 ccaggattgc ataatgactc ttatccgttt aatcggggcg caaggatagc aaaagcttta
    11581 cgctaagtta attatattcc ccggtttgcg ttataccgtc agagttcacg ctaatttaac
    11641 aaatttacag cattgcaaag atgaacgccg tataatgggc gcagattaag aggctacaat
    11701 ggacgccatg aaatataacg atttacgcga cttcctgacg ctgcttgaac agcagggtga
    11761 gctaaaacgt atcacgctcc cggtggatcc gcatctggaa atcactgaaa ttgctgaccg
    11821 cactctgcgt gctggtgggc ctgcgctgtt gttcgaaaac cctaaaggct actcaatgcc
    11881 ggtgctgtgc aacctgttcg gtacgccaaa gcgcgtggcg atgggcatgg ggcaggaaga
    11941 tgtttcggcg ctgcgtgaag ttggtaaatt attggcgttt ctgaaagagc cggagccgcc
    12001 aaaaggtttc cgcgacctgt ttgataaact gccgcagttt aagcaggtgt taaacatgcc
    12061 gacaaagcga ctgcgtggtg cgccctgcca acaaaaaatc gtctctggcg atgacgtcga
    12121 tctcaatcgc attcccatta tgacctgctg gccggaagat gccgcgccgc tgattacctg
    12181 ggggctgacc gtaacgcgcg gcccgcataa agagcggcag aatctgggca tttatcgcca
    12241 gcagctgatt ggtaaaaaca aactgattat gcgctggctg tcgcatcgcg gcggcgcgct
    12301 ggattatcag gagtggtgtg cggcgcatcc gggcgaacgt ttcccggttt ctgtggcgct
    12361 gggtgccgat cctgccacga ttctcggtgc agtcaccccc gttccggata cgctttcaga
    12421 gtatgcgttt gccggattgc tacgcggcac caaaaccgaa gtagtaaagt gtatttccaa
    12481 tgacctcgaa gtgccagcca gtgccgaaat cgtcctggaa gggtatatcg atcctggtga
    12541 gatggcgccg gaagggccgt atggtgacca cacaggttac tataatgaag tcgataattt
    12601 cccggtgttt accgtgacgc atattaccca gcgtgaagat gcgatttacc attccaccta
    12661 taccgggcgt ccgccagatg agcccgcggt actgggcgtg gcgttgaacg aagtgtttgt
    12721 accgattctg caaaaacagt tcccggaaat tgtcgatttt tacctgccgc cggaaggctg
    12781 ttcttatcgt ctggcggtag tgacgatcaa aaaacagtac gccggacacg cgaagcgcgt
    12841 catgatgggc gtctggtcgt tcttacgcca gtttatgtac actaaatttg tgatcgtttg
    12901 cgatgatgac gtcaacgcac gcgactggaa cgatgtgatt tgggcgatta ccacccgtat
    12961 ggacccggcg cgggatactg ttctggtaga aaatacgcct attgattatc tggattttgc
    13021 ctcgcctgtc tctgggctgg gttcaaaaat ggggctggat gccacgaata aatggccggg
    13081 ggaaacccag cgtgaatggg gacgtcccat caaaaaagat ccagatgttg tcgcgcatat
    13141 tgacgccatc tgggatgaac tggctatttt taacaacggt aaaagcgcct gatgcgcgtt
    13201 tgttttgccc tatttatcga tccgacagag aaagcgcatg acaaccttaa gctgtaaagt
    13261 gacctcggta gcagctatca cggataccgt atatcgtgtc cgcatcgtgc cagacgcggc
    13321 cttttctttt cgtgctggtc agtatttgat ggtagtgatg gatgagcgcg acaaacgtcc
    13381 gttctcaatg gcctcgacgc cggatgaaaa agggtttatc gagctgcata ttggcgcttc
    13441 tgaaatcaac ctttacgcga aagcagtcat ggaccgcatc ctcaaagatc atcaaatcgt
    13501 ggtcgacctt ccccacggag aagcgtggct gcgcgatgat gaagagcgtc cgatgatttt
    13561 gattgcgggc ggcaccgggt tctcttatgc ccgctcgatt ttgctgacag cgttggcgcg
    13621 taacccaaac cgtgatatca ccatttactg gggcgggcgt gaagagcagc atctgtatga
    13681 tctctgcgag cttgaggcgc tttcgttaaa gcaccttggt ctgcaagtgg tgccggtggt
    13741 tgaacaaccg gaagcaggct ggcgtgggcg tactggtacc gtgttaacgg cggtattgca
    13801 ggatcatggt acgctggcag agcatgatat ctatattgcc ggacgttttg agatggcgaa
    13861 aattgcccgt gacctgtttt gcagtgagcg taatgcgcgg gaagatcgcc tgtttggcga
    13921 tgcgtttgca tttatctgag atataaaaaa acccgcccct gacaggcggg aagaacggca
    13981 actaaaactt tattcagtgg catttagatc tatgtcgcat ccggcaattg gtgcacgatg
    14041 cctgatgcga cgctggcgcg tcttatcagg cctacattgg tgccggatcg gtagaccgga
    14101 taaggcgttc acgccgcatc cggcaagtgg ttaaacccgc tcaaacaccg tcgcaatacc
    14161 ctgacccaga ccgatacaca tcgtcgccag accaaactga acgtctttgc gttccatcag
    14221 attcagcagc gtggtgctga tacgcgcacc ggaacaaccc agcggatgac ccagcgcgat
    14281 cgcaccacca ttgaggttga tcttctcgtc aatctgctcc attagcccga gatctttaat
    14341 gcacggcagg atctgcgcgg caaaggcttc gttcatctca aacacgccga tatcgctggc
    14401 agaaagcccc gcttttttca gcgccagctt cgaggccgga accgggccgt aacccataat
    14461 cgatgggtca caaccaacga ccgccatcga acgcacacga gcgcgcggct taagacctaa
    14521 ttcatgggcg cggctttcac tcatcaccag catggcagct gcgccatcgg aaagtgcaga
    14581 agatgtgccc gccgttaccg taccgtttac tggatcaaac gccggacgca gcgtggcgag
    14641 ggcttccacg gtggtttccg ggcgaatcac ttcgtcgtaa ttaaactgct tcaggacgcc
    14701 gtcggcatcg tgaccaccag ttgggatgat ttcattttta aatgcggccg actgcgtggc
    14761 ggcccaggcg cgggcgtgtg accgcgcggc aaaggcatcc tgcatttcac ggctgatacc
    14821 gtgcatacgc gccagcattt ctgccgttaa gcccatcatg cccgccgctt tggcgacatt
    14881 gcggctcagg ccggggtgaa aatcgacgcc gtgactcatc ggcacatggc ctatatgctc
    14941 cacgccgcca accagacatg cctgcgtatc gccagtcatg atcattcgtg ctgcgtcatg
    15001 cagtgcctgc atggatgaac cacacaagcg attgacggta accgccggga cagagtgcgg
    15061 cacttctgcc agcagcgccg cgttacgggc gatgttaaaa ccctgctcca gcgtctgttg
    15121 cacacaaccc cagtaaatat cgtcgagggc cgccgcttcc agcgccgggt tacgcgccag
    15181 caggctacgc attaaatgag cggagagatc ttccgcacgc acgttacgaa aagcaccgcc
    15241 cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg acaacctgtt ccattgtgac
    15301 tccttaagcc gttttcaggt cgccaaccgg acgggctggc tcaaccggag gatagtacgg
    15361 ttcgttatga cgcgctttat tacgcagacc ttccggcact tcatacagcg ggccgaggtg
    15421 ctgatattgc tgtgccatat cgaggtattt tgcgctaccg agggtgtcca gccagcggaa
    15481 cgcgccgccg tggaacggag ggaagcccag gccgtagacc agcgccatat ccgcttccgc
    15541 cggagtggcg ataatgccct cttccagaca gcgcaccact tcgttgacca tcgggatcat
    15601 catgcgggcg ataatctctt cttcgctgaa atcgcgcttc ggttggctca cttctgccag
    15661 caggtcttca acggcggcgt cttcttcttt cttcggctta cctttgctgt cttctttata
    15721 acgccagaaa ccgaggccgt tcttctgacc aaagcggttg gcatcaaaca gcgcgtcgat
    15781 ggcatcgcgg taatctttct gcatccgctg cgggaagcct gctgccatga cagcctgagc
    15841 gtgatgcgcg gtatcaatgc ccacaacgtc cagcagatat gccgggccca tcggccagcc
    15901 aaactgtttt tccatcactt tgtcgatctt gcggaaatcc gcgccgtcgc gcagcagctg
    15961 gctgaaaccg gcgaaatacg ggaacagcac gcggttaaca aagaagccgg ggcagtcgtt
    16021 aaccacaatc ggcgtcttgc ccatcttgct cgcccaggcg acaactttcg cgatggtttc
    16081 gtcggagctt ttctcgccgc gaataatttc taccaacggc attcggtgga ccgggttaaa
    16141 gaagtgcatc ccgcagaagt tttccgggcg ttccagcgcg ttggccagtt cgctgatagg
    16201 aatggttgaa gtgttagacg ccagcacggt atcctggcgt actttttgtt cggtttctgc
    16261 cagtacggct tttttcactt tcgggttttc aacaaccgct tctaccacaa tatccacgcg
    16321 gtcaaatccg gcgtagtcga gcgttgggtg gatcgtggag atcacgccag ccagtttcag
    16381 accatcgatt ttgccgcgct caagctgctt gttcagcagt ttcgcggctt cggtcatgcc
    16441 gagggttaac gacttgtcgt tgatatcttt catgacaacc ggcacgcctt tccacgcgga
    16501 ctggtaagcg ataccgccac ccatgatgcc tgcgcccagc acagcagcct gtttcggggt
    16561 ttcaacgtct ttggtgagtt tcttcgcttt gccttttaca tattgatcgt taaggaaaat
    16621 gccgaccagt gcgcgagctt cgttggtatg cgccagcggg acaaaacttt tgttttccag
    16681 atttaaggct tcttcacgac caaaacgggc cgcagcttca atggttttta ctgcggtgat
    16741 gggggccgga taatgtttcc ccgccgtttg cgcgaccatt cctttagcga tggtgaagct
    16801 catggtggct tcaatcttgc tcagttttag cggttcctgc ttcggctgac gtttcgcttt
    16861 ccagtcgagg tcgccgttaa tggcctggcg taaaatcgcc atcgcccctt caatcagttt
    16921 ttctgctttg actacgccat ccaccagacc gattttcagc gcctgatccg cgccgacatc
    16981 tttaccggcg gcaatgattt ccagtgcact gtcagcgccc agcatacgtg gcatacgtac
    17041 agaaccgcca aagcccggca tgatgcccag tttggtttcc ggcagaccga tgcgcagatc
    17101 cggcgtcgcc agacgataat cggtcgccag cacgcattcg cagccaccac ccagcgcata
    17161 gccgttgacg gcagcaatgg tcggcaccgg cagatcttcc aggcgattaa acacgctatt
    17221 ggcaaagtgc agccactgac ttaactgttc ttcaggaacg aggaacaggg acaaaaattc
    17281 ggtgatatca gcaccgacga taaaggctgc tttgttcgaa cgcagcagca gcccttttag
    17341 atctgattgc tgttccagca cgccgatggc ctcgccgagg ctggcgacgg tcgcagtgtc
    17401 gagtttatta actgaacctg gggcatcaaa taccagttcg gcaatgccat cttccagcca
    17461 gtcaaggtac agggtgtcgc ctttgtaaag catgtcagtc tcctgaatcc gcaaggtaat
    17521 ctggtcgtac cagatgcatc ggagtgtgtg ttttgtgtta aaaatatgca aataaaagat
    17581 taaagaaatg ccgatctgat cacgctcggc agaaatcacg ctctggatga acgatgtgct
    17641 aagatgcgga gacttaaggt caaaaaaaca gaagggtaaa aaatggaatc actggcctcg
    17701 ctctataaaa atcatatagc taccttacaa gaacggactc gcgatgcgct ggcgcgcttc
    17761 aagctggatg cattacttat tcactccggc gagctgttca acgtttttct cgacgatcat
    17821 ccctatccgt ttaaagtgaa cccgcaattc aaagcgtggg tgccggtaac tcaggtgcca
    17881 aactgctggt tgctggtgga tggcgtgaac aagccgaaac tgtggttcta tctgccggtt
    17941 gattactggc acaacgtcga accgctgccg acctccttct ggactgaaga tgtggaagtg
    18001 atcgcgctgc cgaaagccga tggcattggt agcctgctgc ctgctgcgcg cggcaatatc
    18061 ggttatatcg gtccggtgcc ggaacgtgcg ctgcaactgg gtattgaggc cagcaacatc
    18121 aacccgaaag gggtgatcga ctacctgcat tattaccgct ccttcaaaac cgagtacgaa
    18181 ctggcctgta tgcgtgaagc gcagaaaatg gcggtcaatg gtcatcgtgc ggcagaagaa
    18241 gcgttccgtt ctggcatgag cgagttcgat atcaatattg cctatctgac cgcgaccggt
    18301 catcgtgata ccgacgtacc ttacagcaac attgtggcgc ttaacgaaca cgctgcggtg
    18361 ttgcattaca ccaaactgga ccaccaggca ccggaagaga tgcgcagctt cctgctggat
    18421 gccggggccg aatataacgg ctatgcggct gacctgaccc gtacctggtc ggcaaaaagc
    18481 gacaacgact acgcacagct ggtgaaagac gtaaatgatg aacaactggc gctgatcgcc
    18541 accatgaaag caggcgtcag ctatgtggat taccacatcc agttccatca gcgcatcgcc
    18601 aaactgctgc gtaaacatca aatcatcacc gatatgagtg aagaagcgat ggtcgaaaac
    18661 gatctcaccg gaccgtttat gccgcacggt attggccatc cgctgggcct gcaggtgcat
    18721 gacgtcgccg gttttatgca ggatgatagc ggtacacacc tcgcggcacc ggcaaaatat
    18781 ccgtacctgc gctacacccg tattctccag ccgggcatgg tgttaaccat cgaaccgggt
    18841 atctacttca ttgaatcgct tctggcaccg tggcgtgaag ggcagttcag caagcacttc
    18901 aactggcaga aaattgaagc actgaaaccg ttcggcggca ttcgtatcga agacaacgtg
    18961 gtgatccacg aaaacaacgt ggaaaacatg acccgggatc tgaaactggc gtgatggaaa
    19021 gctggttaat tcctgcggca ccggtcacgg tcgttgaaga gatcaaaaag agccgcttca
    19081 taacgatgtt ggcgcatacc gatggcgttg aggcggcgaa agcgtttgtt gaatcggtgc
    19141 gggcagaaca ccccgatgcc cgccaccatt gcgtggcgtg ggtggcgggt gcgccggatg
    19201 attctcaaca gctgggtttc tctgacgacg gggagccggc gggaacggca ggtaaaccga
    19261 tgctcgccca gctaatgggc agcggcgtcg gggaaattac cgctgtggta gtgcgctact
    19321 acggcggcat attgcttggc accggtgggt tagtgaaagc gtatggcggc ggcgtgaatc
    19381 aggcgctgcg ccagctaacg acccaacgca agacgccatt aaccgaatat actttgcaat
    19441 gtgaatatag tcagttaacc ggcattgaag cgttgctggg gcagtgtgac ggcaaaatta
    19501 tcaacagtga ttatcaggca ttcgttctgc tgcgggtggc gcttccggcg gcgaaagtgg
    19561 ctgaattttc cgcaaagctg gcggatttta gccgtggttc attgcaattg ttagcgattg
    19621 aagaataatc cccacttcgt tttgcagaac taaggaagcg gcagagatgc attttcgcgc
    19681 cattacccga atcgttggac tactggtcat cttattttca gggaccatga ttatccctgg
    19741 gctggtagca ctcatctacc gggatggagc gggccgcgct tttacccaga ccttttttgt
    19801 cgccctcgcc attggctcta tgctgtggtg gccgaaccgc aaagagaagg gcgaactgaa
    19861 atcccgtgag gggtttctga tagtggtgct gttttggacc gtgctgggta gtgtcggtgc
    19921 gctccctttt atcttctcgg aaagcccgaa cctcacgatt accgatgcgt tttttgaatc
    19981 tttctctggc ctgaccacca ccggggccac tacgctggtg gggctggatt cgctccctca
    20041 tgccatcctc ttttatcgcc agatgctgca atggtttggc gggatgggga tcatcgtgtt
    20101 agcggttgcg atactgccta tcctcggcgt gggtgggatg cagctctatc gcgcagaaat
    20161 gcccggcccg ctgaaagata acaaaatgcg cccgcgaatt gcggaaacgg cgaaaactct
    20221 gtggttgatt tatgtcttgc tgaccgtcgc ctgtgcgctg gcgttgtggt ttgccggaat
    20281 ggatgccttt gatgccatcg gccatagctt tgcgactatc gctattggcg gcttctcgac
    20341 acatgatgcc agtatcggtt atttcgacag tccgactatt aacactatca ttgctatctt
    20401 cctgctgatc tccggctgta actacggtct gcacttttca ctgttaagtg ggcgtagtct
    20461 gaaggtttat tggcgcgatc cggaatttcg catgtttatc ggcgtacagt ttacgctggt
    20521 ggttatttgt acactcgtac tgtggtttca taatgtctac agttcggcgc tgatgacaat
    20581 taaccaggcg tttttccagg tggtatcgat ggcgacaacc gccgggttta cgactgacag
    20641 cattgcccgc tggccgctct ttttgccggt actgctttta tgttcagctt ttatcggcgg
    20701 ttgcgccggg tcaacgggcg gtggcctgaa agtgatccgc atcctgctgc tgtttaagca
    20761 ggggaaccgt gagctgaaac gactggtgca tccgaacgcc gtctatagca ttaagctggg
    20821 gaatcgcgca ctgccggaac gtatcctcga agccgtttgg ggatttttct ccgcctatgc
    20881 attggtgttt attgtcagta tgctggcgat tatcgccacg ggcgtggatg atttttctgc
    20941 ctttgcctca gttgttgcga cattgaataa cctggggcca gggcttggcg tggttgctga
    21001 taactttacc agtatgaacc cggtggctaa atggatcctg attgccaaca tgctgtttgg
    21061 tcgtctcgag gtctttacat tactggtgct ctttaccccg actttctggc gtgaatgatg
    21121 gagtaatacg tgaaaacatt aattcttttc tcaacaaggg acggacaaac gcgcgagatt
    21181 gcctcctacc tggcttcgga actgaaagaa ctggggatcc agacggatgt cgccaatgtg
    21241 caccgcattg aagaaccaca atgggaaaac tatgaccgtg tggtcattgg tgcttctatt
    21301 cgctatggtc actaccactc tgcgttccag gaatttgtca aaaaacatgc gacgcgactg
    21361 aattcgatgc cgagcgcctt ttattccgta aacctggtgg cgcgcaaacc ggagaagcgt
    21421 actccacaga ccaacagcta cgcgcggaag tttctgatga actcgcaatg gcgtcccgat
    21481 cgctgcgcgg tcattgccgg ggcgctgcgt tacccacgtt atcgctggta cgaccgtttt
    21541 atgctcaagc tgattatgaa gatgtcaggc ggtgaaacgg atacgcgcaa agaagttgtc
    21601 tataccgatt gggagcaggt ggcgaatttc gcccgagaaa tcgcccattt aaccgacaaa
    21661 ccgacgctga aataagcata aagaataaaa aatgcgcggt cagaaaatta ttttaaattt
    21721 cctcttgtca ggccggaata actccctata atgcgccacc actgacacgg aacaacggca
    21781 aacacgcagc cgggtcagcg gggttctcct gagaattccg acagagaaag caaaaataaa
    21841 tgcttgactt tgtagcggga aagcgtatta tgaatacccc gcgccg
//
LOCUS       DAZXUK010000037        21719 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZXUK010000037 DAZXUK010000000
VERSION     DAZXUK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21719
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.037"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..214
                     /locus_tag="ACH3L8_001194"
     CDS             <1..214
                     /locus_tag="ACH3L8_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084386.1"
                     /translation="KNAECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLE
                     IKPEPEQQVLIQSLYDRASGSASATQ"
     gene            complement(274..765)
                     /gene="yajQ"
                     /locus_tag="ACH3L8_001195"
     CDS             complement(274..765)
                     /gene="yajQ"
                     /locus_tag="ACH3L8_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFT4084387.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            933..1844
                     /gene="panE"
                     /locus_tag="ACH3L8_001196"
     CDS             933..1844
                     /gene="panE"
                     /locus_tag="ACH3L8_001196"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFT4084388.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            1807..2397
                     /gene="yajL"
                     /locus_tag="ACH3L8_001197"
     CDS             1807..2397
                     /gene="yajL"
                     /locus_tag="ACH3L8_001197"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFT4084389.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(2451..3899)
                     /gene="thiI"
                     /locus_tag="ACH3L8_001198"
     CDS             complement(2451..3899)
                     /gene="thiI"
                     /locus_tag="ACH3L8_001198"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFT4084390.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQAAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4105..4347
                     /gene="xseB"
                     /locus_tag="ACH3L8_001199"
     CDS             4105..4347
                     /gene="xseB"
                     /locus_tag="ACH3L8_001199"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFT4084391.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4347..5246
                     /gene="ispA"
                     /locus_tag="ACH3L8_001200"
     CDS             4347..5246
                     /gene="ispA"
                     /locus_tag="ACH3L8_001200"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFT4084392.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            5271..7133
                     /gene="dxs"
                     /locus_tag="ACH3L8_001201"
     CDS             5271..7133
                     /gene="dxs"
                     /locus_tag="ACH3L8_001201"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFT4084393.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            7188..8105
                     /gene="yajO"
                     /locus_tag="ACH3L8_001202"
     CDS             7188..8105
                     /gene="yajO"
                     /locus_tag="ACH3L8_001202"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFT4084394.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(8216..8734)
                     /gene="pgpA"
                     /locus_tag="ACH3L8_001203"
     CDS             complement(8216..8734)
                     /gene="pgpA"
                     /locus_tag="ACH3L8_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFT4084395.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(8712..9689)
                     /gene="thiL"
                     /locus_tag="ACH3L8_001204"
     CDS             complement(8712..9689)
                     /gene="thiL"
                     /locus_tag="ACH3L8_001204"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFT4084396.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(9767..10186)
                     /gene="nusB"
                     /locus_tag="ACH3L8_001205"
     CDS             complement(9767..10186)
                     /gene="nusB"
                     /locus_tag="ACH3L8_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFT4084397.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(10206..10676)
                     /gene="ribE"
                     /locus_tag="ACH3L8_001206"
     CDS             complement(10206..10676)
                     /gene="ribE"
                     /locus_tag="ACH3L8_001206"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFT4084398.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(10765..11868)
                     /gene="ribD"
                     /locus_tag="ACH3L8_001207"
     CDS             complement(10765..11868)
                     /gene="ribD"
                     /locus_tag="ACH3L8_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFT4084399.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(11872..12321)
                     /gene="nrdR"
                     /locus_tag="ACH3L8_001208"
     CDS             complement(11872..12321)
                     /gene="nrdR"
                     /locus_tag="ACH3L8_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFT4084400.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            12472..13011
                     /locus_tag="ACH3L8_001209"
     CDS             12472..13011
                     /locus_tag="ACH3L8_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFT4084401.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            13309..14193
                     /gene="tsx"
                     /locus_tag="ACH3L8_001210"
     CDS             13309..14193
                     /gene="tsx"
                     /locus_tag="ACH3L8_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFT4084402.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(14370..14717)
                     /gene="yajD"
                     /locus_tag="ACH3L8_001211"
     CDS             complement(14370..14717)
                     /gene="yajD"
                     /locus_tag="ACH3L8_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFT4084403.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(14846..15817)
                     /gene="secF"
                     /locus_tag="ACH3L8_001212"
     CDS             complement(14846..15817)
                     /gene="secF"
                     /locus_tag="ACH3L8_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFT4084404.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(15828..17675)
                     /gene="secD"
                     /locus_tag="ACH3L8_001213"
     CDS             complement(15828..17675)
                     /gene="secD"
                     /locus_tag="ACH3L8_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFT4084405.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(17703..18035)
                     /gene="yajC"
                     /locus_tag="ACH3L8_001214"
     CDS             complement(17703..18035)
                     /gene="yajC"
                     /locus_tag="ACH3L8_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFT4084406.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18058..19185)
                     /gene="tgt"
                     /locus_tag="ACH3L8_001215"
     CDS             complement(18058..19185)
                     /gene="tgt"
                     /locus_tag="ACH3L8_001215"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFT4084407.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(19240..20310)
                     /gene="queA"
                     /locus_tag="ACH3L8_001216"
     CDS             complement(19240..20310)
                     /gene="queA"
                     /locus_tag="ACH3L8_001216"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFT4084408.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            20403..20976
                     /gene="acpH"
                     /locus_tag="ACH3L8_001217"
                     /pseudo
     CDS             20403..20976
                     /gene="acpH"
                     /locus_tag="ACH3L8_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(20981..>21719)
                     /locus_tag="ACH3L8_001218"
     CDS             complement(20981..>21719)
                     /locus_tag="ACH3L8_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFT4084409.1"
                     /translation="AAKETQPEAYIVGEHFGDARQWLQADVEDAAMNYRGFTFPLWGF
                     LANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTLLGRDIA
                     RLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQDTALFALYRRMIV
                     LRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEVVLPASPFLNAVQ
                     WQCKEGHGQLTDGILALPAISATVWMN"
BASE COUNT         5358 a         5825 c         5455 g         5081 t
ORIGIN      
        1 aaaaaatgca gaatgcatgg cacacattac ctttacgctt aataaattaa caacacaacc
       61 agcaataaca aaaatagata gcgtgggtaa tgagcaactt tatcagattg gcaaaatcta
      121 ccataagttg gaaattaaac ctgagccaga gcaacaggtt ttaattcagt cgctttatga
      181 ccgggcatca ggaagcgcct ctgcaactca ataaatgcat ggtcaaaagg gccaccacgg
      241 tggccctgac aatcagtagc tatatccaga aagttaatcg cggaagtttt tgaactggaa
      301 cggctgaccg agatcgccac cgcgcaccat cgccatgaca gcctgcaaat catcacgaga
      361 tttgccagtc acgcggatct catcgccctg aatttgcgcc tgcactttca gtttgctgtc
      421 tttgatcatc ttgacgattt ttttctgagt ggcgctctca atgccctgtt tcagtttcgc
      481 ttccacaaac caggttttac cgctatgaac gatattttcc ggtacatcca gtgaactgcc
      541 ttcaatgccg cgcttcagca gcttggcacg cagaatatcc agcaactgat tgacctggaa
      601 gtcggactcg ctcaacactt tgatggtttt gctggcgtcg ttcagctcaa atgaggcttc
      661 aacgttacgg aagtcaaaac gggactccac ttcgcgactc gcgttatcga ccgcgttacg
      721 tgcttcctga agatcaactt cagagacaat atcgaaagat ggcatctttt cctctccctt
      781 catttttgat gcgaagcata atacctgcaa agttaaataa ccaacagtcc tgcaccagga
      841 cgtatccctt aattgtgcat agtcgcaaca gcatgcacaa ttaagggata gccttattga
      901 acacccggag tggttgcggg tgaggaggaa caatgaaaat taccgtattg ggatgcggtg
      961 ccttagggca attatggctt acagcacttt gtaaacaggg tcatgaagtt cagggctggc
     1021 tgcgtgtacc gcaaccttat tgtagtgtga atctggttga gacagatggt tcgatattta
     1081 acgaatcgct gaccgccaac gatcccgatt ttctcgccac cagcgatctg ctcctggtga
     1141 cgctgaaagc atggcaggtt tccgatgccg tcaaaagcct cgcatccaca ctgcctgtaa
     1201 ctacgccaat actgttaatt cacaacggca tgggcaccat cgaagaattg caaaacattc
     1261 agcagccatt actgatgggc accaccaccc atgccgcccg ccgcgacggc aatgtcatta
     1321 ttcatgtggc aaacggaatc acgcatatcg gtccggcacg gcaacaggat ggcgattaca
     1381 gttatctggc ggatattttg caaaccgtgt tgcctgacgt tgcctggcat aacaatattc
     1441 gcgccgagct gtggcgcaag ctggccgtca actgcgtgat taatccactg actgccatct
     1501 ggaattgccc gaacggtgaa ttacgtcatc atccgcaaga aattatgcaa atatgcgaag
     1561 aagtcgcggc agtgatcgaa cgcgaagggc atcatacttc agcggaagat ttgcgtgatt
     1621 acgtgatgca ggtgattgat gccacagcag aaaatatctc gtcgatgttg caggatatcc
     1681 gcgcgctgcg ccacaccgaa atcgactata tcaatggttt tctcttacgc cgcgcccgcg
     1741 cgcatgggat tgccgtaccg gaaaacaccc gcctgtttga aatggtaaaa agaaaggaga
     1801 gtgaatatga gcgcatcggc actggtttgc ctcgcccctg gtagtgaaga gactgaagcc
     1861 gtcaccacga tcaacctctt ggttcgcggc ggtatcaaag tcaccactgc cagcgtcgac
     1921 agcgatggca acctggcaat tacctgctcg cgcggcgtga agctgctggc ggatgcgccg
     1981 ctggtcgaag tggctgatgg cgaatatgac gtgatcgtgc tgcctggtgg cattaaaggc
     2041 acggagtgtt ttcgcgatag cactctgctg gttgaaaccg ttaaacagtt ccaccgttcc
     2101 gggcgtatcg tcgcggctat ttgcgccgcg ccagccaccg tgctggtgcc gcacgatatc
     2161 tttccgattg gcaatatgac cggcttcccg acgctgaaag acaaaattcc cgctgaacaa
     2221 tggcaggaca agcgcgtcgt ctgggatgca cgggtaaaat tgctgaccag ccaggggccg
     2281 ggtacagcta tcgactttgg tctgaaaatt atcgacctgc tggttgggcg cgagaaagcc
     2341 catgaagtgg catcacaact ggtgatggcg gcagggattt ataattatta cgagtagtgt
     2401 cggatgcggc aaacgtcgca tctgaccaga tgcgacgtaa aaactacaaa ttacgggcga
     2461 tataccttca cattgttaaa gccttgctcg cgcagataga gcgcctgcag acggctcatc
     2521 accccgcgct cacaccacag cagccaggtt ttgttctggt cgagatcgcc aaatttggtg
     2581 ctcagtttat agaacggcag agaaaccaca tcaatccctt cgactttcag tggcttatct
     2641 tcctgttcat cgacagaacg gatatcgaga atcacgtcgt tcgggccgaa gccgttgacg
     2701 gtttccactt ccaccacttc ctgctcggtc tgctgggcga tttcgcggat atcaacgtta
     2761 ttcgcttcct caaccacttt atcgagaata ctgaagtcga acttctcttc ttccgcttca
     2821 atcttcgatt taaccgcttt caccgtcggg cttttggaga tcacgccaca atattccggc
     2881 atcgtgcgag caaagtcttc ggtgccaatc tggcgagcca ggttgatgat gtgctctttg
     2941 tcgtaagaaa tcagcggacg caggatcagc gtgtcagaga cgttatcaat caggcgcagg
     3001 ttggtcagcg tctgactgga cacctggccg agcgcttcgc cggtgaccag cgcctgtacg
     3061 ccgtaacgtt cagccacttt agatgcggca cgcaccatca tacgtttgag gataacgccc
     3121 atctgaccgt cgtcgatttt ctcgagaatt tccccgacaa ccggttcgaa attaatggcg
     3181 acaaaacgca cgcggtggga gctgccaaag cggttccaca gataatgcgc cgcctgacgc
     3241 acgccaattt catgcgctgc gccgccgagg ttaaagaagc agtaatgcac gcggcagccg
     3301 cgacgcatca acatataact ggaaacaccg gagtcgaaac caccggaaat gagcgacagc
     3361 acatcttcct gggtgccaat cgggaaacca ccaatacctt cgtagcgacc tttaatcagc
     3421 aggaggcgat cgtcttccac ttccagatga acagttacat ccgggttagt cagcttcacg
     3481 cgcgcggatt caatatgctg atttaaaccg ccgccgacgt aacgttccac atcaatcgag
     3541 ctaaaatcat gtttgccacg gcgcttcacg cgtacgcaga aggttttgcc ttccagctga
     3601 tcgcgatact gaaccaacgc tttctcaaaa atatcgtgca tgtcggtaaa cggcacgtct
     3661 tcgacttcga gaatatggtg gatacccgga atacgggtca gggcgtcgcg aatagtcaga
     3721 cgctggtttt catctttcgc gcgaacttcg atgttatccc agtggcggac gacagcgagc
     3781 gtctcatcat agtgctttaa aacgttacga atgttcccgg taaggatttt tataaagcgc
     3841 aagcgcacag attggctttt gatggtgatt tccgggaaca atttaatgat aaacttcatg
     3901 gcggcaatgg ttcgttggca agccttaagc gacttgtata gggaaaaata cagcagccca
     3961 cacctgcggc tgcatccagg cgcggaagta taccactaac atcgctttgc tgcgcacatc
     4021 accttaccat tgcgcgttat ttgctatttg ccctgagtcc gttaccatga cggggcgaaa
     4081 aatattgaga gtcagacatt cattatgccg aagaaaaatg aggcgcccgc cagctttgaa
     4141 aaggcgctga gcgagctgga acagattgta acccgtctgg aaagtggcga cctgccgctg
     4201 gaagaggcgc tgaacgagtt cgaacgcggc gtgcagctgg cacgtcaggg gcaggccaaa
     4261 ttacaacaag ccgaacagcg cgtacaaatt ctgctgtctg acaatgaaga cgcctctcta
     4321 acccctttta caccggacaa tgagtaatgg actttccgca gcaactcgaa gcctgcgtta
     4381 agcaggccaa ccaggcgctg agccgtttta tcgccccact gccctttcag aacactcccg
     4441 tggtcgaaac catgcagtat ggcgcattat taggtggtaa gcgcctgcga cctttcctgg
     4501 tttatgccac cggtcatatg tttggcgtta gcacaaacac gctggacgca cccgctgctg
     4561 ccgtagagtg tatccacgct tactcattaa ttcatgatga tttaccggcg atggatgatg
     4621 acgatctgcg tcgcggtttg ccgacctgcc atgtgaagtt tggcgaagca aacgcgattc
     4681 tcgctggcga cgctttacaa acgctggcgt tctcgattct aagcgatgcc aatatgccgg
     4741 aagtgtcgga tcgcgacaga atttcgatga tttctgaact ggcgagcgcc agcggtattg
     4801 ccggaatgtg cggtggtcag gcattagatt tagacgcgga aggcaaacac gtacctctgg
     4861 acgcgcttga gcgtattcat cgtcataaaa ccggcgcatt gattcgcgcc gccgttcgcc
     4921 ttggtgcatt aagcgccgga gataaagggc gtcgtgctct gccagtactc gacaagtacg
     4981 cagagagcat cggccttgcc ttccaggttc aggatgacat cctggatgtg gtaggagata
     5041 ctgcaacgtt gggaaaacgc cagggtgccg accagcaact tggtaaaagt acctaccctg
     5101 cacttctggg tcttgagcaa gcccggaaga aagcccggga tctgatcgac gatgcccgtc
     5161 aggcgctgaa acaactggct gaacagtcac tcgatacctc ggcactggaa gcgctagcgg
     5221 actacatcat ccagcgtaat aaataaacaa taagtattaa taggcccctg atgagttttg
     5281 atattgccaa atacccgacc ctggcactgg tcgactccac ccaggagtta cgactgttgc
     5341 cgaaagagag tttaccgaaa ctctgcgacg aactgcgccg ctatttactc gacagcgtga
     5401 gccgttccag cgggcacttc gcctccgggc tgggcacggt cgaactgacc gtggcgctgc
     5461 actatgtcta caacacccca tttgaccaat tgatttggga tgtgggacat caggcttatc
     5521 cgcataaaat tttgaccgga cgccgcgaca aaatcggcac catccgtcag aaaggcggtc
     5581 tgcacccgtt cccgtggcgc ggcgaaagcg aatatgacgt attaagcgtc gggcattcat
     5641 caacctccat cagtgccgga attggtattg cggttgctgc cgagaaagaa ggcaaaaatc
     5701 gccgcaccgt ctgtgtgatt ggcgacggcg cgattaccgc tggcatggcg tttgaagcga
     5761 tgaatcacgc gggcgatatt cgtcctgata tgctggtggt cctcaacgac aatgaaatgt
     5821 cgatttccga aaatgttggc gcgcttaaca accatctggc tcagctgctt tccggtaagc
     5881 tttactcttc actgcgcgaa ggcgggaaaa aagttttctc tggcgtgccg cccattaaag
     5941 agctgctcaa acgtaccgaa gaacatatta aaggcatggt ggtgcctggc acgttgtttg
     6001 aagagctggg atttaactac atcggcccgg tggacggtca cgatgtgctg gggcttatca
     6061 ccacgctaaa gaacatgcgc gacctgaaag gcccgcagtt cctgcatatc atgaccaaaa
     6121 aaggtcgtgg ttatgaaccg gcagaaaaag acccgatcac tttccacgcc gtgcctaaat
     6181 ttgatccctc cagcggttgt ttgccgaaaa gtagcggcgg tttgccgagc tattcaaaaa
     6241 tctttggcga ctggttgtgc gaaacggcag cgaaagacaa caagctgatg gcgattactc
     6301 cggcgatgcg tgaaggttcc ggcatggtcg agttttcacg taaattcccg gatcgttact
     6361 tcgacgtggc tattgccgag caacacgcag tgacctttgc cgccggtctg gcgattggtg
     6421 gatacaaacc cattgtcgcg atctactcca ccttcctgca acgcgcctat gatcaggtgc
     6481 tgcatgacgt ggcaattcaa aagctaccgg tcctgttcgc cattgaccgc gcgggcattg
     6541 ttggtgctga cggtcaaact caccagggcg cttttgatct ctcttacctg cgctgcatac
     6601 cagaaatggt cattatgacc ccgagcgatg aaaacgaatg tcgccagatg ctctataccg
     6661 gctatcacta taacgatggc ccgtccgcgg tgcgctaccc gcgcggcaac gcggttggcg
     6721 tggaactgac gccgctggaa aaactaccaa ttggcaaagg cattgtgaag cgtcgtggcg
     6781 agaaactggt gatccttaac tttggtacgc tgatgccaga aacggccaaa gtcgccgaat
     6841 cgctgaacgc tacgctggtc gatatgcgtt ttgtgaaacc acttgatgaa gcgttaattc
     6901 tggaaatggc cgccagccat gaagcgctgg tcaccgtaga agaaaacgcc attatgggcg
     6961 gcgcaggtag cggagtgaac gaagtgctga tggcccatcg taaaccagta cccgtgctga
     7021 acattggcct gccggacttc tttattccgc aaggaactca ggaagaaatg cgcgccgaac
     7081 tcggcctcga tgccgccggt atggaagcca aaatcaaggc ctggctggca taatccctac
     7141 tccactcctg ctatgcttaa gaaattattc atggcaggag tggcagcatg caatacaacc
     7201 ccttaggaaa aaccgacctt cgcgtttccc gactttgcct cggctgtatg acctttggcg
     7261 agccagatcg cggtaatcac gcatggacac tgccggaaga gagcagccgt cccataatta
     7321 aacgcgcgct ggaaggtggc ataaatttct ttgataccgc caatagctat tccgatggta
     7381 gcagcgaaga gatcgttggt cgcgcactgc gggatttcgc ccgtcgtgaa gacgtagtcg
     7441 ttgccaccaa agtgttccat cgcgttggtg atttaccgga aggattatcc cgtgcacaaa
     7501 ttttgcgctc tatcaacgac agcctgcgac gtctcggcat ggattatgtc gatatcctgc
     7561 aaattcatcg ctgggattac aacacgccga tcgaagagac gctggaagcc ctgaacgacg
     7621 tggtaaaagc cgggaaagcg cgttatatcg gcgcgtcatc catgcacgct tcgcagtttg
     7681 ctcaggcgct ggaactacaa aaacagcact gctgggcgca gtttgtcagt atgcaggatc
     7741 actataatct gatttatcgc gaagaagagc gcgagatgct gcctctgtgt tatcaggagg
     7801 gcgtggcggt gattccgtgg agcccgctgg cgcgggggcg actgacgcgt ccatggggag
     7861 aaactaccgc acgactggtg tctgatgagg tggggaaaaa tctctataaa gaaagcgatg
     7921 aaaatgacgc gcagatcgca gagcggttaa cgggcgtcag tgaagaactc ggtgcaacac
     7981 gagcacaagt tgcgctggcc tggttgttaa gtaaaccggg cattgccgca ccgattatcg
     8041 gaacttcgcg ggaagaacag cttgatgagc tgctgaacgc ggtggatatc actttgaagc
     8101 cataacagat tgccgagctg gaaacgccgt ataaaccgca tgcggtcgta ggatttaaat
     8161 aagagtccag gcctgatgag acgtgacaag cgtcacatca ggcatcggtg cacaactacg
     8221 acagaatacc cagcggccag tgatgaccga taaagtacag gatgcctgcg gaaatcaccc
     8281 cggcgacaat atcgtcgatc atgatcccca tgccgccatg tacattgcga tcaaaccagc
     8341 ggatcggcca cggcttccac atatccagaa tacggaaaat cacaaacccg gcggcaaccc
     8401 actgccagtc attggtcggc agcgccatga gcgtgatcca cataccaata aattcgtccc
     8461 agacaatgct gccgtgatcg tgcacgccca tatctttcgc cgtttgatga caaagataga
     8521 cgccgataca gatccccaac atcaccacca gcgagtagag ctgccagggt aaaaaggtca
     8581 tcagatacca gaaagggatc gccgccagcg atcccatcga gccaggaacg attgggctta
     8641 atccacttcc gaatccgaca gcaagcagat gccacggatt actcatcttc aggcgacttt
     8701 tcgcgacatc tttatggcgt gacaaaatgg tcatatcctt tccagtcaaa cgtgacaggt
     8761 tcgccgtcac gaataaaaca aagcccttcg atatcggcgg tcatttgccc gatacaggta
     8821 aacggtacgc caaggtgtcc gagagcaaca tccagcgcgc cacggttcag ttccggcacc
     8881 gtgaaacaca actcgtaatc ttcaccgcca gagagcgccc agcgcagcgc ctgttccggt
     8941 tcaacatggc gagaaagcgc atcagaaaac ggcagcaatg ccaggtcaat acgtgcgccg
     9001 cagtcgctgg ctttcacgat atgcccgaga tcggaaatca gaccgtcaga gagatcgata
     9061 gctgaatttg ccagatcgcg cagtgcctgc ccctgtaaaa tacgcggcga tggacggaga
     9121 tgacgtttga tcaagtagtc agcatcttta gcatcggcaa cctgcaaacg gttttgcaaa
     9181 atcgccagcc cggcggcgct atcgcccggt gtaccggtca catagatcca gtcacccggt
     9241 ttcgccccag agcgcgttaa ggctcgtccc atcggaacaa agccgtggat acccaacgtc
     9301 attgataatg gcccacgcgt ggtatcgccg ccaatgagtt gcatatcgta ataattgaga
     9361 agatcaaaca aactgtcgct gaaggactca agccacgctt cgtctacgtc cggtaaggtt
     9421 aatgccagcg tcagccaggc cggatcggcc cccatcgctg ccagatcgct taggttcacc
     9481 gccagtgctt tataagccag atcagcagga tcgatatcag ggaggaaatg gttgcccgcc
     9541 accagcgtat cagtgctgat cgccagggtc tgtttctcgg ggatattgag aagagcgcaa
     9601 tcgtcgccga tgcccagttc gacatcaaga cgagaacttc ttacacggtc aaaataacgg
     9661 gcaatcaggg agaactcgcc acatgccata cgttatgcct cagcaggtaa aaaagaaaag
     9721 gccggcgatc atggaacagt cttccgtgaa tctaccggcc tggatattac tttttgttag
     9781 ggcgaatcac aggtgctgct ttatcgagta cgccgttgac gaacttatgg ctgtcttctg
     9841 cgccgaacga tttcgccagt tcgatcgctt cgttaatggc cactttgtat ggcacatcac
     9901 tacgtttaga cagttcgtac agcgcaatgc gcagtactgc tttttctacc tggcccagtt
     9961 cttccagcag gcgggacagg tagggcttca tcagtccgtc gaggtatgcg gtgttagtcg
    10021 ccaccccggc cagcagctca cggaagtaca ggacgtcaac gtcttttaca tcctgttcag
    10081 ccaggaactg gtattcaaca tcagcgatgt cgttctggga caactgccag gagtagagcg
    10141 cctggacggc acactcacga gcgcggcgac gagcagcagg tttcacggat ttccccttac
    10201 taatttcagg ccttgatggc tttcaataca ttaatcattt caagcgcggt cagtgcagct
    10261 tctgcacctt tgttgccagc tttggtgcca gcacgttcga tcgcttgttc aatgctttca
    10321 gtggtcagaa ccccaaaagc aaccggaatt tcgctgtcct gggcaacatg cgccaggccg
    10381 ttgcttgcac caccagcgac atattcaaag tgggcagtgc caccacgaat aaccgtaccc
    10441 agcgcaatca ccgcgtcgta tttaccggtt ttagccagtg cacccgccgc cagcggcagc
    10501 tcataggcac caggcaccca aacaacggta atgttttcat cttttacctg accgatacgt
    10561 ttcagtgcgt caattgcacc ttccagcagg ctgtcattga taaagttgtt gaaacgcgcg
    10621 atggtgatgg cgacgcgagc gtccggggta gcaacgttag cttcaataat gttcattttc
    10681 ttccttcggg ttcgagtatg gccccgcagg ggggcggatt ttagcataat atttcgtgcg
    10741 ctgcttccct ttcgagccgg gagatcatgc acccactaaa tgcaggcaaa catccgggcc
    10801 tacatgacgt atctctttga atttaaattg gggggcgtcg gctaatttct caagccctgg
    10861 cagcgagcat aatccacggg cgtcgctgcc taatagttta ggtgcgatat agacaatcag
    10921 ctcatcgact aaacccgcct gtagcaatac gccagcgagc gttggccccg cttccaccca
    10981 gatgctgtta atctgctgtt tacccagttg catcatcagt acaaccagat ccagatgacc
    11041 tttatgctct ggaatcagca aggtacgcac cgtttccggc cactcacgag aatcttcctg
    11101 cgtacgcgcg aaccaggttt cgccgggctg ctgcacaatg cgatgttccg gcgtcacgcg
    11161 attttggcta tcaatcacaa tacgtactgg ctggcggaga ttttgttgcg ggtagagcgc
    11221 ctgagtttgt tcatccagtt cagaccaacg cactgttaag gctggatcat ccgccagcac
    11281 cgtggcgctg ctggttaaaa tggcatgact ttgtgcgcgc tgtcgttgta cgtcgcgccg
    11341 cgcctgaggc gaagtgatcc actggctttc gccgctcgcc atcgccgtgc ggccatcaag
    11401 cgatgcgcca agttttaact gaatataagg aaagccggtg cgcatccgct tgagaaagcc
    11461 tttattcaat tgctcggctt cactcatcat cagcccgtgg ctgacgtcaa tgccagcctg
    11521 ttgcagacgg taaagtccac gcccagcgac ctgcgggtta ggatcttgca tcgctgcgac
    11581 cacgcgcgct acgccagcgg cgatgagtgc gtcacagcac ggtggcgtac ggccatgatg
    11641 gctacagggt tcgagtgtca catacgccgt tgcgcctttg gctttttcac ccgccatacg
    11701 caacgcgtgt acttcggcat gtggttcacc cgctcgttgg tggtaacctt caccgacaat
    11761 ttcgccatct ttgacaatga cgcacccgac attcgggttg gggtgggtgg taaaacgtcc
    11821 tcgttgcgcc agctttagcg cccgcgccat gtaatactcg tcctgcacgg cttagtcctc
    11881 caggcgcgcg atctcttcgc caaattcttt gatatcttcg aaactgcggt agacagaggc
    11941 aaaacggata taggcgactt tatcgagctt tttcaattgc tccatcacca gattgccaat
    12001 catcttgctc ggtacttcgc gctcaccggt ggcgcgcagc tgcgatttaa tatgattgat
    12061 tgccatttca acgtaatcgg aactcaccgg acgtttttcc agcgcccgca gcattccgct
    12121 acgcaatttc tcttcattaa acggttcacg cacgtcgttg ctttttacaa cacgcggcat
    12181 aaccagctcc gccacttcaa aggtggtgaa acgttcatta cacaccagac actgccgacg
    12241 gcggcgtacg gatgaaccct cgcccacgag acgagagtca attaccttag tgtccacggc
    12301 gaaacagaat gggcaatgca tacggcgtcc tgaccaggtg gttaacggaa atctatttta
    12361 ccctgaactg acgatacaac aaaggcgcaa cgcttttgag ataaaggatc gatgctttcg
    12421 ctgcttttac cctctaccat taggctaatt gcgacgagaa agggacgacc tatgaatact
    12481 aacgtttttc gactgctcct gctgggaagc ctgttcagcc ttagcgcctg tgtgcagcaa
    12541 agtgaagtgc gacagatgaa acacagcgtc agcacgctga accaggagat gacgcaactc
    12601 aatcaagaaa cagtcaaaat cactcagcaa aacaggctga atgcaaaatc cagcagtggg
    12661 gtttaccttc tgcctggagc gaaaacaccg gcaagactgg aaagccagat cggtacttta
    12721 cgtatgtcgc tggtgaatat tacgcctgat acagatggca ccacccttac actacgtatt
    12781 cagggtgagt cgaatgatcc cttgcccgca ttcagcggaa ctgttgaata tgggcaaatt
    12841 cagggaacaa tagacaactt tcaggaaatc aatgtgcaga atcaattgat taatgcccct
    12901 gccagcgtcc tcgcccccag cgatgttgat attccgttac aattaaaggg gatttctgtg
    12961 gatcagttag gctttgtacg tatccacgac attcaacctg ttatgcagta aacgtatttc
    13021 gggacgattt tgtgcgtccc gcaacatctt ccccgtcatt ttgttactct gcttacatca
    13081 cctggattga tagtaaaagt ttgcaacaag ggcgaaagtc agtacaatcc ccgcccgaat
    13141 gtgtgtaaac gtgaacgcaa tcgattacgt aaatgataga actgtgaaac gaaacatatt
    13201 tttgtgagca atgattttta taataggctc ctctgtatac gaaatattta gaaacgcaat
    13261 ttgcgccttt ttcactcccg caagggattt tcaaacagtg gcatacatat gaaaaaaaca
    13321 ttactggcag ccggtgcggt actggcgctc tcttcgtctt ttactgtcaa cgcagctgaa
    13381 aacgacaaac cgcagtatct ttccgactgg tggcaccaga gcgttaacgt tgtcggaagc
    13441 tatcacaccc gtttcggacc gcagatccgc aacgatacct accttgagta cgaagcattc
    13501 gctaaaaaag actggttcga cttctatggt tatgcggatg cgccggtatt cttcggcggt
    13561 aactccgatg ctaaaggtat ctggaaccac ggttctccgc tgtttatgga aatcgaacca
    13621 cgtttctcca tcgacaagct gaccaatact gaccttagct tcggtccgtt caaagagtgg
    13681 tacttcgcga acaactacat ttacgacatg ggtcgtaata aagatggtcg ccagagcacc
    13741 tggtacatgg gtctgggtac cgatatcgac actggcctgc cgatgagcct gtccatgaac
    13801 gtctatgcga aataccagtg gcagaactat ggcgcagcga acgaaaacga gtgggacggt
    13861 taccgtttca aaattaaata ctttgtgccg attaccgatc tgtggggcgg tcagctgagc
    13921 tacatcggct tcaccaactt cgactggggt tccgatttag gggatgacag cggtaacgca
    13981 atcaacggta ttaagacccg tactaataac tctatcgctt ccagccatat tctggctctg
    14041 aactacgatc actggcacta ctctgtcgta gctcgttact ggcacgacgg tggtcagtgg
    14101 aacgacgatg cagaactgaa cttcggcaac ggcaacttca acgttcgctc taccggctgg
    14161 ggtggttacc tggtagtagg ttacaacttc tgattatgaa aatgccggga tttattcccg
    14221 gcatttctga ttgttaacaa cgtgtgcttt gttcatgccg gatgcggcgt aaacgctttg
    14281 tccggcccac aaaagcacgt aaattcaata tattgctgaa ataacgtagg cctgataagc
    14341 gtagcgcatc aggcaatttt acgttttaat cacttcttct tgttcatcat cgctttcagg
    14401 tcagcgaatg ggttgtactt cgcttcaccg acatctttct gcgcatcttc ccctgcgata
    14461 acggtcgtac catactgatc cgcttcggta tatttcgaat gctcatgatc gtggcaatag
    14521 agacacaaca attcccagtt actgccatct tccgggttat tggtatggtc gtgatcaatg
    14581 tggtgaacgg taagttcacg caggttagaa taaacaaact cgcgggaaca gcgaccgcag
    14641 acccacggat agatttttaa tgctttttcg cgatagccgc tttctaaccg tgcatagttt
    14701 tttgggatga tagccatgaa aagttacctg gatgaaatgt agggttacgc gcagtttccc
    14761 aagatgcggg gattttcgca atctccatac atcaaaaaaa atcccgatct tctgaccggg
    14821 attttcaaca tcaacgggaa cttgattaag gcagaattga cggctgatcc gccccttctt
    14881 tttccacttt ctgctgcaac atgtgttcgc gcttcatacc cagtttcaga gccaacgcag
    14941 atgctacgta gatggaagat gcagtgccga tggaaacacc gataagcatg gtcagcgaga
    15001 agccttccag taccggacca ccgaagagat acagcatcag gataaccatc aaggtagtac
    15061 cggatgtgat caaggtacgg tgcagcgtct gggtcaagga cacgttaaag atttcgtaag
    15121 gcgtaccgcg acggatcttg cggaagtttt cacgaattcg gtccgatacc acgatactgt
    15181 cgttaagcga gtaaccgata accgacatca acgatgccac aatggtcagg tcaatctcga
    15241 tatggaataa cgacaaaata cccagcgtaa tgataacgtc gtgcgtcagc gcaataacca
    15301 cccctgccgc cagtcgccac tcaaagcgga aacctacgta cacgaggata gacagcagcg
    15361 ctgccatcaa cgccatcgca ccggtttgcg caaggtcagc ccccacgctc ggaccgacga
    15421 actcaatacg cttcactgct gcattctgat tggtggattc gttaatcacc ttcagaactt
    15481 ggctgcccag cacctgaccg ccggtttcgc cttcagcagg cggcatacgg accatgatgt
    15541 cgtggctgct accaaagttt tgcagcattg gctcttcaaa accggctttt tgcaatgcat
    15601 cacgcattac gtcaatttca gccggttttt cgagcgtaat ttcgataacc gtaccaccgg
    15661 tgaaatccag cccccagtta aagccgcgca cgcccataat aacgatagca gcgattaaca
    15721 gcagaccaga gatgccgaaa gcccagtagt cccagcgcat aaagtcatag actttacggc
    15781 cgtggtttag ttgttcaaca gtatattcct gtgccacatc gcactcctca gattgacagc
    15841 ttcttgacgc gcttgccgcc atatagcagg tttacgatgg cacgcgtacc gacaatcgcg
    15901 gtaaacatcg acgtcgccac accgataccg gtagtaatcg cgaacccttt aattgccccg
    15961 gtacccactg cgtacaggat gatgacttta atcagcgtgg tgatgttcgc atcgaagata
    16021 gaactgaatg cgccacgata accttcatca attgcctgtt gaacagtacg tccgttgctc
    16081 aactcttctt taatacgttc gttgatcagt acgttcgcat cgaccgctac cgcaagggtt
    16141 aagacgatac ccgcgatacc aggcatactc agtgttgcgc ctggcaacag cgacataata
    16201 ccgacgatca agatcaagtt ggcaatcaga gcactggtcg caatcagacc aaacttctta
    16261 tagaagatga tcatgaacag aatagaaacc agcagaccgg caaggcaagc ctccagcccc
    16321 tgctcaatgt tctgcatacc cagggtcggg ccaattgtac gttcttcaac aatctgaatc
    16381 ggcgcgatca acgcaccggc acgcagcagc agtgacagct gacgggcttc gttcgggttg
    16441 ttgatgccgg tgatacggaa gctgttaccc agacgagact ggatgttggc gatgttaatc
    16501 acctcttcct gtttcaccag aaccgcacga ccatttgcat ctttcttacc gctgtctttg
    16561 tactccacaa acagggttgc catcggtttg ccgatgttgt ccttggtgaa gttagacata
    16621 atgttgccac cagcgctatc gagcgagatg ttaacctgcg gctggttgta ttcgtcctgg
    16681 ctggaagtgg agtcggtgat atggtcacca gtcagaatta cgcgtttgta cagcacaacc
    16741 ggctgacctt cgcgggtctg tttcacttca gagtcgcccg gtacgcgacc ggatgccgca
    16801 gcggcctgat caacattggt gtttaccaga cggaattcca gcgttgccgt cgcgcccaga
    16861 atctctttcg cacgcgcagt gtcctgaata ccgggcagtt caacaacgat acggtcagca
    16921 ccctgacgct gaaccaccgg ctccgccacg ccaagttggt ttacacggtt acgcaggata
    16981 ttaatgttct gctgcaccgc atattcacgc gcttcactca gacgagcatc gctcattacc
    17041 gcacgcagct ggttgctgcc ctggctgcta atcaccaggt ccggatggcg cttgctcaga
    17101 tacgcaatgg cttcatcacg agctttagca tcgcggaaag tgatgctcag gccgtagttg
    17161 ttttctttac gaacagtggt atacgggatg cccttttcgc gcaggtcact gcgtaggcta
    17221 tcgatatttt gttcctggag tttgccaagc gcggtgtcca tatccacttc catcaggaag
    17281 tgaacgccgc cacgcaggtc aaggccgagc ttcatcggct cagcatgaat agctgccagc
    17341 cagcgcggcg ttgccggggc aaggttaagc gccacgacgt atttgtcacc cataacgccc
    17401 attaatgctt cacgagcgcg caactgggtg tcagtggagt cgaagcgcgc aagaatagcg
    17461 ccctcttcca gtgccacaga cttagcagtt attttttctt cttgtaacgt tttctggacc
    17521 tggatcagcg tttgctcact ggcggcgact ccgcgcgcac cagtgatctg aacagccgga
    17581 tcctcaccaa acaggttggg aagcgcatac agcagaccga tgacaatcac cacgatcagc
    17641 atgacgtact tccacaaagg ataacggttt aacacggcaa ttcccttagg gaaaaatttt
    17701 aattacagcg ccttcatggt gcctttcggc aggacggcag ctacgaagtc acgtttaata
    17761 actacttcag tggtgtcatt cagcgcgata gcaatgtagc cgttttccgc tactttggtt
    17821 acgcgaccaa ccaggccacc gttcgtcaga acttcatcac ctttggcaat ggagtccatc
    17881 agctttttgt gttctttggt gcgcttctgc tgtggacgca ggatcatgaa atagaaaatc
    17941 agaccgaata ccaccagcat caaaatcaaa gacatcgggc taccttgcgc cggtgcaccc
    18001 gttgccgcta ccgcatcaga aataaaaaag ctcattaaat ttccctcatt attaatatta
    18061 atcaacgttc aaaggtggta cttctcgccc ctgacgctgg taaaaatcag ttacgaagct
    18121 ctctaattta ccctcttcaa tagccttgcg taaacccgcc atcaaacgct ggtagtaacg
    18181 aaggttatga atggtgttga gtcgcgcgcc taatatttcg ttgcaacggt cgagatgatg
    18241 caagtaagcg cgtgaataat tgcgacaggt gtagcaatca cactcaggat cgagtgggcc
    18301 agtatcgctc ttatatttcg cattgcggat tttcaccacg ccatcggtca cgaacaaatg
    18361 accatttcgg gcgttgcggg ttggcattac gcagtcaaac atatcgatac cgcgacgtac
    18421 gccttcaacc aggtcttccg gtttaccaac gcccatcagg taacgcggtt tgtctgccgg
    18481 aatttgcgga cacacatgct ccagaatacg gtgcatatct gctttcggct cacccacagc
    18541 cagaccgccg acagcgtagc catcaaagcc gatatctacc agacctttaa cagatatatc
    18601 acgtaaatct tcgtaaatac tgccctgaat tataccaaat aacgcatttt tgttaccaag
    18661 actgtcaaaa cgctcacggc tacgcttcgc ccaacgcaga gacatctcca tagagcgttt
    18721 tgcgtaatcc cagtcagcag gatacggcgt acactcatca aagatcatga cgatatccga
    18781 accaagatcg tactgaatct ccatcgactt ttccgggtcg aggaaaatcg ggtcgccgtt
    18841 gatcgggtta cggaagtgaa cgccctgttc ggtgatttta cgaatatcac caaggctgaa
    18901 gacctggaag ccgccggagt cggtaagaat cggtcctttc cactgcataa aatcgtgcag
    18961 atcgccgtgc agtttcatga tttcctggcc cgggcgcagc cacaggtgga aggtgttgcc
    19021 gaggataatt tgcgcgccag tggcttcaac ttcttccggc gtcatccctt ttacggtgcc
    19081 gtaggtgcca acaggcataa aacaaggcgt ttccactacg ccacgatcaa agaccaggcg
    19141 gccacggcgt gcgcgaccgt cggtggtgtc cagttcaaat ttcatttttt ctccactacg
    19201 tcagaaaaac agtccaacgt ttaaaccagc gccgcggaat tactccccga cgcgctcatt
    19261 aattgcctgc ggattgtacg tgataaacat cgcatcaccg taactaaaaa agcgatattt
    19321 ctcttctacc gccgctttat aggcattcat ggtgtgttga taaccggcaa aggccgaaac
    19381 cagcataatc agcgtcgact ctggcaagtg gaagttcgtc accagcgcat cgaccacttt
    19441 gtactggaag cccggataga taaagatttg ggtatcgtca aagaacggtt cgataagatc
    19501 gttttttgcc gcctgtgccg cgctttccag cgaacgtact gaagtggtgc caaccgcaat
    19561 cacccggtta ccgcgcgctt tcgccgccag taccgcgtct accacatcct gcggtacttc
    19621 agcgtattcc gagtgcatga tgtgatcttc aatggtgtcg acgcgcaccg gctggaaggt
    19681 gcccgcacca acgtgcaacg tcacaaacgc catctccacg cctttggcgc gtaatttttc
    19741 cagcaaaggc tcgtcaaaat gcagaccagc ggtcggggct gcaaccgcgc ccggtttttc
    19801 gctataaacg gtttgataaa gttcgcggtc agcgtcttcg tccggacgat cgatatacgg
    19861 cggcagcggc atatggccga tgctgttgag gatatccagt accgagcgtt catcattaaa
    19921 ttcgacttca aacagtgcgc cgtggcgcgc ggtcattgtt gcgttgatac tttcgtcatc
    19981 gcccagcaac agttctgcgc caggttttgg cgctttcgag gcgcgaatat gcgcaagaat
    20041 gcgtttgtcg tcgagcatcc gctcaaccag cacttcaatc ttgccgccgc tggctttacg
    20101 cccaaacaga cgcgccggga tcacgcgggt attattaaaa accagaagat cgccggggtt
    20161 gagcttatcg agtaaatcgg tgaaagtacc gtgcgtcagc gcgcccgtcg gcccgtccag
    20221 cgacagtaaa cgacagctac tgcgttcagg catgggatag tgggcaatca gggattcggg
    20281 caattcaaag gagaaatcgg taacgcgcat gacactgact cagactaaaa taagagtcgg
    20341 gtagtctagt gccggggcgc tctccctgca acctttaccc ccctttggat agaatataat
    20401 cgatgaattt tttagctcac ctgcatttag cccatctcgc ggaaagctcg ctttctggca
    20461 atttactggc tgatttcgta cgcggaaatc ctgaagaaag ttttccgccc gacgtcgtgg
    20521 ctggcattca tatgcatcga cgtattgact gacaatctgc cggaagtccg cgaagcacag
    20581 gagtggtttc gtagtgaaac gcgccgcgtt gcgcctatta cgctggatgt catgtgggat
    20641 cactttcttt cccgccactg gtcgcagctg tcgccggact ttccgctaca ggaatttgtc
    20701 tgttatgccc gcgagcaagt gatgacgatt ttgccggact caccgccacg ttttatcaat
    20761 ctgaacaatt acttgtggtc agagcagtgg ctggtgcgct atcgcgatat ggatttcatc
    20821 cagaacgtgt taaacggcat ggcaagccgc cgcccacgtc tggatgccct gcgtgactcc
    20881 tggtacgatt tagacgctca ttatgacgcc ctcgaaaccc gcttctggca gttttatccg
    20941 cggatgatgg cgcaggcgtc acacaaggcg ttataaaacg ttagttcatc cataccgtag
    21001 ccgaaatggc aggcaaagcc agaatcccgt cagtcagttg cccatgccct tctttgcatt
    21061 gccattgcac ggcattgaga aacggtgacg cgggtagcac cacttcactg gcctcgccac
    21121 ggttgattgc caccagtaca cgttgctgat tcagcacgcg gacaaatacc actacgttat
    21181 cttccgcata cagcacctga cagccgccac gacgcagcgc ctgacttttc ttacgcagca
    21241 caatcattcg ccggtacagc gcgaataacg ccgtatcctg cttttccacc tgccagggga
    21301 acggtttacg gcaaaacgga tcgtttttac catccagtcc cacttcatca ccgtaataaa
    21361 tgcacggtac accaggccag gtgaacagcc agaccaccgc cagcggcagg cgcgcaatat
    21421 cccgaccgag cagcgtttta aatcgcgcag tatcgtggct atcgagctga ttaaacatac
    21481 gtaattgttg ttgatgagaa agccctgcgc ggtaattatc catccaggcc atacaggttt
    21541 gggcgtcaat ttgctgcgga tcataagaga tgtcggtatt ggcaagaaat ccccacaacg
    21601 ggaatgtgaa gccacgatag ttcatggcag catcttccac atcggcctgt aaccattgcc
    21661 gtgcatcgcc aaaatgttcg ccgacaatat acgcttccgg ctgggtttct ttcgccgct
//
LOCUS       DAZXUK010000038        21396 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZXUK010000038 DAZXUK010000000
VERSION     DAZXUK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21396
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.038"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1264
                     /gene="uidC"
                     /locus_tag="ACH3L8_001219"
     CDS             <1..1264
                     /gene="uidC"
                     /locus_tag="ACH3L8_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFT4084410.1"
                     /translation="RKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRAD
                     KPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGDK
                     SFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALGY
                     WALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDTS
                     RYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETAL
                     VSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGANY
                     RVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKGR
                     EKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(1490..2998)
                     /locus_tag="ACH3L8_001220"
     CDS             complement(1490..2998)
                     /locus_tag="ACH3L8_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFT4084411.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(3099..4274)
                     /gene="manA"
                     /locus_tag="ACH3L8_001221"
     CDS             complement(3099..4274)
                     /gene="manA"
                     /locus_tag="ACH3L8_001221"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFT4084412.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            4473..6119
                     /gene="fumB"
                     /locus_tag="ACH3L8_001222"
     CDS             4473..6119
                     /gene="fumB"
                     /locus_tag="ACH3L8_001222"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFT4084413.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            6262..7665
                     /gene="fumC"
                     /locus_tag="ACH3L8_001223"
     CDS             6262..7665
                     /gene="fumC"
                     /locus_tag="ACH3L8_001223"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFT4084414.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(7662..8591)
                     /gene="tus"
                     /locus_tag="ACH3L8_001224"
     CDS             complement(7662..8591)
                     /gene="tus"
                     /locus_tag="ACH3L8_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFT4084415.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(8667..9968)
                     /gene="rstB"
                     /locus_tag="ACH3L8_001225"
     CDS             complement(8667..9968)
                     /gene="rstB"
                     /locus_tag="ACH3L8_001225"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFT4084416.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(10147..11466)
                     /locus_tag="ACH3L8_001226"
     CDS             complement(10147..11466)
                     /locus_tag="ACH3L8_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFT4084417.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(11691..12434)
                     /gene="rstA"
                     /locus_tag="ACH3L8_001227"
     CDS             complement(11691..12434)
                     /gene="rstA"
                     /locus_tag="ACH3L8_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFT4084418.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            12563..12898
                     /locus_tag="ACH3L8_001228"
     CDS             12563..12898
                     /locus_tag="ACH3L8_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFT4084419.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(12895..13617)
                     /gene="folM"
                     /locus_tag="ACH3L8_001229"
     CDS             complement(12895..13617)
                     /gene="folM"
                     /locus_tag="ACH3L8_001229"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFT4084420.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(13654..15036)
                     /locus_tag="ACH3L8_001230"
     CDS             complement(13654..15036)
                     /locus_tag="ACH3L8_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFT4084421.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(15222..16166)
                     /gene="ydgH"
                     /locus_tag="ACH3L8_001231"
     CDS             complement(15222..16166)
                     /gene="ydgH"
                     /locus_tag="ACH3L8_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFT4084422.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            16690..18222
                     /gene="pntA"
                     /locus_tag="ACH3L8_001232"
     CDS             16690..18222
                     /gene="pntA"
                     /locus_tag="ACH3L8_001232"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFT4084423.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            18233..19621
                     /gene="pntB"
                     /locus_tag="ACH3L8_001233"
     CDS             18233..19621
                     /gene="pntB"
                     /locus_tag="ACH3L8_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFT4084424.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(19646..20680)
                     /gene="tqsA"
                     /locus_tag="ACH3L8_001234"
     CDS             complement(19646..20680)
                     /gene="tqsA"
                     /locus_tag="ACH3L8_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFT4084425.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            21091..>21396
                     /gene="mdtJ"
                     /locus_tag="ACH3L8_001235"
     CDS             21091..>21396
                     /gene="mdtJ"
                     /locus_tag="ACH3L8_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFT4084426.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     "
BASE COUNT         5516 a         5082 c         5434 g         5364 t
ORIGIN      
        1 gagaaaaata gtggccatgg ccgttatttg cctgacggct gcctctggcc ttacctctgc
       61 ttacgcggcg caactggctg acgatgaagc gggactacgc atcagactga aaaacgaatt
      121 gcgcagggct gataagccca gtgctggcgc gggaagagat atttacgcat gggtacaggg
      181 aggattgctc gatttcaata gtggttatta ttccaatatt gttggcgttg aaggcggagc
      241 gtattatgtt tataaattag gtgctcgtgc tgatatgagt acccggtggt atcttgatgg
      301 tgataaaagt tttggctttg ccctgggggc agtaaaaata aaacccagtg aaaatagcct
      361 gcttaaatta ggtcgcttcg ggacggatta tagttatggt agcttacctt atcgtattcc
      421 gttaatggtt ggcagttcgc aacgtacatt accgacagtt tctgaaggag cattaggtta
      481 ttgggcttta acgccaaata ttgatctgtg gggaatgtgg cgttcacgag tatttttatg
      541 gactgattca acaaccggta ttcgtgatga aggggtgtat aacagccaga cgggaaaata
      601 cgataaacat cgcgcacgtt cttttttagc cgccagttgg tatgatgata ccagtcgcta
      661 ttctctgggg gcatcggtac agaaagatgt ttccaatcag atacaaagta ttctcgagaa
      721 aagcataccg ctcgacccga attatacgtt gaaaggggag ttgctcggct tttacgcgca
      781 gctcgaaggt ttaagtcgta ataccagcca gcccaatgaa acggcgttgg ttagtggaca
      841 attgacctgg aatgcgccgt ggggaagcgt atttggcagt ggtggttatt tgcgccatgc
      901 aatgaatggt gccgtggtgg ataccgacat tggctatccc ttttcattaa gtcttgatcg
      961 taaccgtgaa ggaatgcagt cctggcaatt aggtgccaac taccgtgtaa ctccgcaatt
     1021 tacgctgaca tttgcaccga ttgtgactcg cggctatgaa tccagtaaac gagatgtgcg
     1081 gattgaaggc gcgggtatct taggtggtat gaactatcgg gtcagcgaag ggccgttaca
     1141 agggatgaat ttctttcttg ctgccgataa agggcgggaa aagcgcgatg gcagtacgct
     1201 gggcgatcgc ctgaattact gggatgtgaa aatgagtatt cagtatgact ttatgctgaa
     1261 gtaagaaata acgccggaga gaaaaacctc cggcgtttca gattgttgac aaagtgcgcg
     1321 ttttttatgc cggatgcggc gtaaacgcct tatccagcct acaaaaactc ataaattcaa
     1381 agtgttgcag gaaaaggtag gcctgataag cgtagcgcat caggcaatct ctggtttgtt
     1441 ttcagatgaa aacgccggag tgaaaattct ccggcgtttt ggccgtgaat tactgctgcg
     1501 gaattgccgg tacagccgga acgttaagag ctggcatcgc aaacatgcca acaaaatctt
     1561 ctaacgacat tttctgccca tttaacgtta tctgaccgtt agcatattgc aggctggtgg
     1621 tgatggtatt gtcctgcaag gtggtcagac ggaacatctg ccccattgct gatgcacctt
     1681 caacttgctg tttcgccagt tttttcgctt gatcttcctg ataaccttcc agcttcgcta
     1741 cctgagtcat caactcagtt gccatatcca ccggaatggt cagtttcgca tccagagatt
     1801 taaccgaacg atctacttcc tgcgccagcg tttgcggcgc ttctttagtc gttgccggat
     1861 ctttcaggaa cagcgacaga ttcagcgcac tttcaccctg actgtttttc cagcttagcg
     1921 gcgcgatagt aatcaccgga tcgcctttca acatcagcgg cagggcgcta aagaaggctt
     1981 ccgtcacttt ctcctgataa agttcgggat tgttggcaat ttctggctgt gccagcagcg
     2041 cctgagtttg cgcgttatat tgctggctaa actgatgcca ggcttcacca tcaatctggc
     2101 cgacttttaa agtcagcttg ccgctgccca gatcctgatt ctgtaccttc aggctgttta
     2161 gcgagtaatc cagttggcta ttgatcgttt taccgtcatt gaccagatcc gatttaccgc
     2221 tgatctccat gccttccagc agtgccagtt ctttgccttc cactgaaatg gtcatttttt
     2281 ccagtgacag tttttggttt cctacgcgct caccaaaact tcccagcgtg ctggaaccgt
     2341 cggttttcag attattaaag gtcaactgca ctttctggtt gtattcgtta actgcgtcta
     2401 tccgaccact ttgcgcctct ccggaaaggg agatggcttt gccgtctctg tcagcattta
     2461 actggaactc gccgccgcta aaggcgactt tttcatcctt ttgctcgtaa ttcagtggat
     2521 tgagcgaaat atcggaactg gaatcaccgc tgtaaccaat gcgcgagtta atctcaaaag
     2581 gcgtttcacc ttttgccata tcaaacagtg gtttgcttac ttcgttatta accagcgtgg
     2641 tttgaattga tgccatcgac gggatcaggt tcagtttttt aagctgggca agcgggaagg
     2701 gaccatgatc aaccgattcg ttgaagatga cgctctgacc gcttttaatc cacggatttt
     2761 ctttcccggc aatgggtttc accaacagtt gcaactggct gctgaatacg ccgcgatgat
     2821 agttttgata actcacttcc aggttggatt caggagctgt cagtttgagt tgcgcgttcg
     2881 cctgcgcgac catgtcttcg agatgggttt caatcttctt gcctgtatac catgcgccgc
     2941 ctgtccagac tacgcctagc gcaacaatga cgcctaccgc taccagcgat ttattcataa
     3001 tgattatcca taaaatgaaa tcaggcggac tggccgcctg aaggtgttat aagcctttaa
     3061 taagcttagc aagagatgtt aattttttca gtaagctctt acagcttgtt gtaaacacgc
     3121 gctaaacggc cgtggccttt gacagtcacc ggtgattcgt tggcggcaat aaacgctgat
     3181 tcacccggtt taagctgtaa ctgctgagaa cctttccaca acgttgcatc gccttcgacg
     3241 cagaacaaaa tggcggcact ctgctggctg atggtggttt ctttatcact aaggtcatgc
     3301 agcgagaagg caaaatcatc cacaggaatc gggaagtcca gttctgcacc ttgtttcacc
     3361 ggctgggtca acaactggtt agccggtttg gcttcgaatt tcacattggc aaccagttcc
     3421 ggaatatcaa tgtatttagg cgtcagacct gcacgcagca cgttatcgga gttcgccatc
     3481 acttccagcg ccacgccttg caggtaagcg tgcggtgttt cagcgaacag gaacatcgct
     3541 tcgccaggat tcaatttcac cacattcagt aacagcgggg agaacaggcc gctgtcttcc
     3601 gggtaaaatt cagaaattaa acgaatcgtt tgccacggtt caccctgctg actgtcgagc
     3661 gccgatttta aaatcgccag cgcgcgggat ttttcttcac cctgcatatt caacaggttg
     3721 gcgaacagtt cgcttaaacg ttcggcatgt ggctgttgta aaaagtgagc aatcgccgga
     3781 tgtgcaccgg cgaccggctg gagtagggag acaatctcgg aaaattcacg aaacgcgttc
     3841 atcgcaagga aaggcgtcag cgcaaaaacc agctccggct tgtggttagg atctttatag
     3901 ttacgctcgg cggcatccat cgggatacct gcggcatttt ctttggcaaa accgatttca
     3961 gaattgtgtt tgtttggatg aacctgaatg gagagtggct gtgctgcgca taatactttg
     4021 aacaggaaag gcagttcgcc aaagcgtttg gcaacggcct ctccgagcag agtcgattta
     4081 tcactctcaa tcacatcacg cagtgaaacg atatctccgg cggcattctg cactcgtgaa
     4141 ctgcttttcg gatgtgcgcc catccacagc tcggccatcg gctggctgga cggattttcc
     4201 ataccataaa gttcagtcaa cgccgttttg ctaccccagg catagttttg cactgagtta
     4261 atgagttttt gcatgatcaa tccctgtttt aatgtggaaa ttaatcccgc tattaaagca
     4321 agaatcctac gggaagtaac ctggagccgc aaaaagtcgt actagtctca gtttttgtta
     4381 aaaaactgtg taggatattg ttactcgctt ttaacagggc aacggaacac ccgcccagag
     4441 cataaccaaa ccaggcagta agtgagagaa caatgtcaaa caaacccttt cattatcagt
     4501 ctccttttcc actcaaaaaa aatgatactg agtattacct gctaaccagt gaacacgtta
     4561 gcgtatctga atttgaaggg caggagattt tgaaagtcgc acccgaagcg ttaactctgt
     4621 tggcgcgtca ggcgtttcat gatgcgtcgt ttatgctgcg tccggctcac caacaacagg
     4681 tggccgacat tctgcgtgac ccggaggcca gcgaaaatga taaatatgtg gcgctgcaat
     4741 tcctgcgtaa ctccgacatc gcggcgaaag gcgttctgcc aacctgtcag gataccggca
     4801 ccgcgattat tgttggtaaa aaagggcagc gtgtatggac cggtggtggt gatgaagcgg
     4861 cgctggcgcg cggtgtctat aacacttata tcgaagataa tctgcgctac tcgcaaaacg
     4921 cgccgctgga tatgtataaa gaagtgaata ccggcaccaa tctgccagcg cagatcgatc
     4981 tttatgccgt tgatggcgac gaatacaaat tcctctgtat cgcaaaaggt ggtggttcgg
     5041 caaacaagac gtatctctat caggaaacca aagcgttact gacgccgggg aaactgaaaa
     5101 attacctggt tgagaagatg cgcacgctgg gtaccgcggc ctgtcctccg tatcatattg
     5161 cgttcgttat tggtggaact tctgcagaaa cgaaccttaa aacggtgaaa ctggcttccg
     5221 cgaaatacta tgatgaactg ccaacggaag ggaatgagca cggtcaggcg ttccgcgatg
     5281 tggaactgga aaaagaatta ctgatcgaag cgcaaaatct cggtctgggt gcgcagtttg
     5341 gtggtaaata cttcgctcac gacatccgcg tgattcgcct gccacgtcac ggcgcatcct
     5401 gtccggttgg tatgggtgtt tcctgctctg ctgaccgtaa tatcaaagcg aagatcaacc
     5461 gtcaggggat ctggatcgaa aaactggaac ataatccagg taaatatatc cctgaagagc
     5521 tgcgtaaagc gggagaaggc gaagcggtgc gcgttgacct taaccgtccg atgaaagaga
     5581 tcctcgcaca gttgtcgcag tatcccgttt ctacacgctt atcgcttaac ggcacgatta
     5641 tcgttggtcg tgatattgct cacgccaaac tgaaagagcg gatggataac ggtgaagggc
     5701 tgccgcaata cattaaagat catccgattt actacgcggg tccggccaaa acgccggaag
     5761 gttatgcctc cggttctctt ggcccaacga ccgccggacg gatggattct tatgtcgatc
     5821 aactgcaagc gcagggcgga agtatgatca tgctggcgaa aggcaaccgc agccagcagg
     5881 tgacggatgc ctgtaaaaaa cacggcggct tctaccttgg cagtatcggt ggtccggccg
     5941 ctgtattggc gcagggaagt attaagagcc tggaatgtgt tgaatatccg gaactgggaa
     6001 tggaagccat ctggaaaatt gaagtggaag atttcccggc gtttatcctt gtggatgata
     6061 aaggaaatga cttcttccag cagatacaac tcacacagtg cactcgctgt gtgaaataaa
     6121 cagagccgcc cttcggggcg gtttttttac atggcacgaa agaccaaaca tttgttatca
     6181 aatagtaaat aatgagtgag ctaaaagttg cttaacgaaa gcaaaacaga aagaaaaaat
     6241 taatcaggtg aggagcaggc catgaataca gtacgcagcg aaaaagattc gatgggggcg
     6301 attgatgtcc cggcagataa gctgtggggc gcacaaactc aacgctcgct ggagcatttc
     6361 cgcatttcga cggagaaaat gcccacctca ctgattcatg cgctggcgct aaccaagcgc
     6421 gcagcggcaa aagttaatga agatttagac ttgttgtctg aagagaaagc gagcgccatt
     6481 cggcaggcgg cggatgaagt actggcagga cagcatgacg acgaattccc gctggctatc
     6541 tggcagaccg gctccggcac gcaaagtaac atgaacatga acgaagtgct ggctaaccgg
     6601 gccagtgaat tactcggcgg tgtgcgcggg atggaacgta aagttcaccc taacgacgac
     6661 gtgaacaaaa gccaaagttc caacgatgtc tttccgacgg cgatgcacgt tgcggcgctg
     6721 ctggcactgc gcaagcaact cattccgcag cttaaaaccc tgacacagac gctgagtgaa
     6781 aaatcgcgtg catttgccga tatcgtcaaa atcggtcgaa cccacttgca ggacgccacg
     6841 ccgctaacac tagggcagga gatttccggc tgggtagcga tgctcgagca taatctcaaa
     6901 catatcgaat acagcctgcc tcacgtagcg gaactggctc ttggcggtac agcggtgggt
     6961 actggactaa atacccatcc ggagtatgcg cgtcgcgtag cagatgaact ggcagtcatt
     7021 acctgtgcac cgtttgttac cgcgccgaac aaatttgaag cgcttgcgac ctgtgatgcc
     7081 ctggttcagg cgcacggcgc gttgaaaggg ttggctgcgt cactgatgaa aatcgccaat
     7141 gatgtccgct ggctggcctc tggcccgcgc tgcggaattg gtgaaatctc aatcccggaa
     7201 aatgagccgg gcagctcaat catgccgggg aaagtgaacc caacccagtg tgaagcatta
     7261 accatgctct gctgtcaggt aatggggaac gacgtggcga tcaacatggg tggcgcttcc
     7321 ggtaactttg aactgaacgt cttccgtccg atggtgatcc ataatttcct gcaatcggtg
     7381 cgcttgctgg cagatggcat ggaaagtttt aacaaacact gcgcagtggg tattgaaccg
     7441 aatcgtgagc gaatcaatca attactcaat gaatcgctga tgctggtgac tgcgcttaac
     7501 acccacattg gttatgacaa agccgcggag atcgccaaaa aagcgcataa agaagggctg
     7561 accttaaaag ctgcggccct tgcgctgggg tatcttagcg aagccgagtt tgacagctgg
     7621 gtacggccag aacagatggt cggcagtatg aaagccgggc gttaatctgc aacatacagg
     7681 tgcagccgtg gaatgatcaa acgaagcggc tgcgcctgag gtttataacg gtgctgcaca
     7741 ttgtcggcat cgtaatttaa caactcacca acgtccggca cgcccgcgcc attatcccga
     7801 ttaatcagtg caatcagtgg tgtagggcag gcgtgttgga cttgtttttg atctcctttg
     7861 taccagacgc gggcaatcgg ctgcaccttc actggacgtt tgatttttaa cttcgcgttc
     7921 tgtggcaggg cagcgatatc ctgatactct cgctccagtt ttctttgcca ctcctcgcgc
     7981 gtccacggtg cgacactgcg tggtgatttc aggctttttt ccagctgtgc caggacttca
     8041 tcacgatgca aattcttaat gatatgttta ttagcccaac caaagcgtaa agtggcgggg
     8101 tcgtgcagaa cggtgagcgt gcggtaagca ttaagggtga tcagccccgg caaatgacga
     8161 tgcacccatt caaaacgtgc cgcggtgggg agttctgatt caaccgtgac gatatgctcg
     8221 aacgtggtct tgagtttatt gatgtgctga atatgactga ccaacgctgc ttgcgaaagg
     8281 ttatcgacct ggtaacacaa cacgccaggc agacggacag cggccttgct gctgcgattt
     8341 tcggactgtt gttgaataaa taaatggcgg aaatgacgca acgccagcga ctgcgcgtcg
     8401 ttgccgagat gttgttttac ctcaatacga ttaagcggat tatgctcatc ctcttttttt
     8461 acctccggca aagagaacac gcgggcaacc aatagcttgt gttgctcaag atgagcggca
     8521 aatgtagcca gctcttgttc catctggcga aaggtagtgt tgagtcggtc tacgagatcg
     8581 taacgcgcca taatattaac cactttagtt acaacatact tattttatag cacagtcgtg
     8641 gtgacttgac catcgtgcgc gtagtgtcag gcagaggtaa attgcgggat gttatgccat
     8701 aacggccagc taaacgagaa gcgagcacca cccagttcgc tgatgtcaca attaaccgta
     8761 ccgcccattg tcagtgctat tgagtggaca attgccagcc ccagcccgca gccgccggtt
     8821 gagcgatccc ggctgggatc gaggcgaaca aaaggttcaa agatatgttc gcggttttct
     8881 ggggcaatcc ctgggccatc atcctcaaca attaatgtcg ctctattccc cgacagtagc
     8941 aggctggttt caaccgttga atggcagtag cgcagggcgt tattgagcaa attatccagc
     9001 acgcgctcca ttaagcgcat atccaacgcc gcataatggc cttgcatgag cgtttttatc
     9061 cgtaccgttt tatcgggcgt tactgcctga atatctgcca gatgcgttga cagccacaac
     9121 ggcaggtctg gttcgctaag atgaagctcg ttttgtgggc gatcgagtcg ggcataagtc
     9181 agcagctctt caattaaagc ttcaagttga ctgatatcac gattcaacgc ctgggattcg
     9241 gcggcgctca ggttatcgct catctccagt cgataacgca ggcgcactaa cggtgttcgc
     9301 agttcgtgag cgataccgtc aataagctgt tttttgctgg caattaaggc gttgatattg
     9361 tccgccatct ggttaaatgc gataccaagt cgttcaaagc tcgaaccctc atcaaagtgg
     9421 atacgttcat tgagatgccc atcgccaaat cgttgcgccg ctgcttccag ttttaacata
     9481 tcctgccagt gcggacgcat ccagataaac accggaaagg cgagggaaat agcaataaaa
     9541 gcgatcaggg cgatatccag caatcgcatt tgatggaggt aataaagata aggaacagga
     9601 ccaactgcca gcacgtagtg gctgcgcggg atacgctgca aaaacgtgta ctgatcgtcc
     9661 agggcgacaa tttcgccgcc acgcagtcgg tgcatggaaa tatcatcaag atggtattta
     9721 ctcagtggct cgacacgcag atcgaaagag agatttaaat ccatctcttt cagcgtctta
     9781 ccccagtcgt gtggggggat ctctcgcaat tcgctgcgca tcagatacag cgaactgttc
     9841 atcaaatcat ccagcgactg tttgcccgcg cgttcggcgg taaatttgta caccagccca
     9901 accagcagag acatcacaag gaagcagaca aacaataaca ggtaaaactg gataaacaat
     9961 tttttcattg gcatcgcccg caacagaata ctattcgtat taattcacga ctgtggagca
    10021 atgtaaaccg ccaactggat aagaccttgt ttggaagata atgtccggaa cagacggatg
    10081 tatccccggt gtattcatat tcagtaaatt caattttcca ccggggcttg tttattatgc
    10141 taaggtttaa tttaacgtga cgctttgttg ttctttagtc tctctatcct taagaagacg
    10201 ggctgatatt acaccggaca ttattgaacc attcacatta agcgctgttc ttgccatatc
    10261 aacgatcggc tctatggcaa taaaaataac tactaatgcg acagggaaat taagtgttga
    10321 aaggacaatc agcgccgcga atgtcccacc actacctacg cctgctaccc ctattgagcc
    10381 aagcgcgatg gcaggtaaaa gagttgcgag gaattgcaat gagaagggat cgatacccat
    10441 tgttggagca atcatcgcca ccatcacggc aggataaatc ccagcgcagc cattttgtcc
    10501 catgctggaa ccaaaagacg ctgcaatatt agcaatcgta tttggaaccc ccaatttttc
    10561 caatgcagaa atagccagag gaattgaagc cgcactactg cgagaaacaa acgcaaaggt
    10621 aagtacttgc cagacggtct tgtaatagtt aacggggttt ttacctatta acagcaaaat
    10681 gcccgagtga acaataaaca tcacaaaaac ggccagataa caggcgacga tgaacccgag
    10741 caaattggta aactgtgaaa gctgatacgt tgagaagact ttcgccatta gtgccattac
    10801 cccataaggc gtcagcgcaa tgacaatacg gaccattttc attacccata tttgaatcgc
    10861 gttgatcccc gcgctcaatt tttcgccctc tgctggtgca tctttcttta cttttaacag
    10921 cgcaatacca gcaagaattg taaagatgac gatgccaata accgaaacac ttcttgcccc
    10981 ggtcagatca aggaagaggt tggttgggat caatgacgta atgagtcgcg gtaaagatac
    11041 cgcagctgtt ttcgagacgt catcgctggt taaagaggat tgcatatgac caaaggcgct
    11101 ggcatcaagg ccaattgcac tggcaatagc caggccaact aaacctgcaa tcatgactaa
    11161 acacaacata caggcaacaa tagttaaaga catcttgcca ataccagcgc tattttcgag
    11221 cttattaatc gctgaaagaa tcgaaatgaa tatgagtgga acggcaacaa gttttaataa
    11281 attaacataa ccgttaccag caatattata ccattcgata acggtttcac tgcttgtctc
    11341 aggaatgaaa tgcagggaga taccaatgat agcccccagg aaaatcgcga taaaaacact
    11401 tttggaaagg gattcgctct tttgatccgt tttgaaaatc ccccatgagc agatcaaaaa
    11461 aagcacaata tatgtgaaag ttgtaaccgt tttttaagcc tcttgcctca ttcagcacct
    11521 gaaatgccgg aaagagtttg ttgtaaaaaa tctgcgccat aagacgcagg gacgaggcga
    11581 ttatagagaa attttttctc attttcatat caaatgtagg gggtttggaa tttgaataag
    11641 ctcttattaa ttgaattcgt taattgttct ctttttcaga tgaacaaata ttagtcatct
    11701 gattgtttat tgtcactatc ccatgcatga ggcgcaaaaa gatagccttt gttacgcaca
    11761 gttttaatgc gataaggttc tgcggcgtta tcgagcagtt tttttcttaa ccgcgaaata
    11821 gccacgtcca cgctacgatc cagtccgtca taactgacgc cgcgtaaatt tttcagcaat
    11881 gcatcgcggt ccatgatttg cccggcatgc gtagctagtt cccacaataa ttcgaaatca
    11941 gctgtcgaga gcgagatttc agtgttagcc agggtgacta cgcggttgat ggggtcgata
    12001 gttaacgtgc cgaaatgcag ggctttgtag ggagtaagag acgtttcctg aagacctttg
    12061 gtcagtgtgg cttgctcatt ctgacgcaaa tgcaaacgta aacgcgctag caaaacagca
    12121 gggggcgtcg ttttgagaat atagtcgcag gcacccattt ccagtgccag gatgtggttc
    12181 atatcgctat cgagagaggt tagaagaaca atcggtccag accactttgc gcgtaaatca
    12241 cgacaaatgg tcatgccgtc cttgcctggt agcatgatgt cgagtaacac caaatccgga
    12301 ttttctcgca aaatggtttc ttcggcctgg tcgccgcgcg gttctacggt aacctgcata
    12361 tcatgttttg ccaggtacgc ggcaatcagt gaaccgactt ccgcatcatc ttccacaaat
    12421 acgatagtgt tcataacatt cacggtagat ataaaaacgt cacaatacac cgcgctgttt
    12481 ttacttacta tcaatcgttt ctaaacaagt tttcatcagg tattctactg atgacttgtt
    12541 atggaagtgt taaggtaaaa agatggggct ggtaatcaaa gccgcgctcg gtgcgctggt
    12601 agtgctgttg attggtgttt tagcaaaaac gaaaaattat tatatcgccg ggctgattcc
    12661 acttttcccg acctttgcgc ttatcgcgca ttatattgtt gccagcgaac gtggcattga
    12721 agccttacgc gcaaccatca tttttagtat gtggtcgatt attccctatt ttgtttacct
    12781 ggtgtcgctg tggtatttca ccggaatgat gcgcttgccc gccgcttttg ttggttctgt
    12841 tgcgtgttgg gggataagcg catgggtgtt gattatatgc tggataaagc tgcattaacg
    12901 cagatgacga ccgccatcaa gtgggaaact gcgtccggtg acaaagcaac tggtaagtaa
    12961 gtaatcgacc aggtcgatca cttctttctc gccaggcgcg gttttcatca gtgatttatt
    13021 cagcgcctgt tgtcgatatt cggcatcatc atgttcatta aacaggatca gcgatggcgc
    13081 aatagaattc actttcactt ccggtgccag cttgcgggca aacgagcggg tcatattatc
    13141 cagtgccgct ttgcttgcag catacgcaac atgtttgtcg ctaccgcgct ccaccacata
    13201 atcggtaaag tgaatgatat cgctggcggc gtgtccgtgc ccacgcagta atctttccag
    13261 cgcatggttg agcaggtatg gggtattaac gtggatctgc atcatgcaag ccagtacgtc
    13321 ggccagtggc gcacccggtt tttccgcaat ccacgcactg gcgttatgca aaatagcacg
    13381 cagaccatgg gtggttttta gtacttcatc ggcaaacgcc atcacaccgt cgttggtcga
    13441 aaaatcagcc tgaatacact gcgcacctgc attaatcagt ccatcaatgg ctggatagtg
    13501 tgtccgatag ctgacaatca ccggttgctt ttgattaatg aaatgccatg cgagggcgag
    13561 gccgatgcga cgacctccgc cagtaattaa tattggcaag ggctgggttt tacccatcgt
    13621 tatctccttt gctatccaac gatgggatga acgttatccc accagcatcc aggtcgccgg
    13681 aactgaggca attaacagca tgccaatcag caccatttcc tgacggttca gtacattatc
    13741 atgtgtatgc gttttgcgcg catacaggaa aaccagcagc cccggtgcat acagaacaac
    13801 ggataacagc aagtgcattg gaccagaggc gtacaataac cataagccat aaatgcaggc
    13861 accgacacct accgctttat gcagtggacg ggttgcgatt ttcagcaaga acgcgccgac
    13921 caggaaatag ggcaccagaa tcatttctga ggcgatggtc agcaacgtgt tgtaatcgga
    13981 accggtcagc cagatgagta ccagacaaat ttgcacgcag atgttggtca accacagtga
    14041 ggcggatggc gcagcttgtg cgttctggcg cgcaaaaatg cgtgggaatg ctttatgagt
    14101 ggcagccagg aacggaactt ctgccgccat gatggtccag cttaagtacg cgccgcaaac
    14161 ggaaacgatc aaaccggcag cgatgatgat ttcgccccat ggtcccatca tttccaccat
    14221 cagaccggcc attgacgggt tacgaatttc agccagttca ggacgtgcca ccacacccag
    14281 cgaaagcagc gttaccagca agtaaacgcc cagagcggag agaaccgcca gcagtgtcgc
    14341 tttgccaaca tcacgtttat tacgcgcacg cgcagaaaca accacggcac cttccacacc
    14401 aatgaaaacc cacagggtga tcagcatggt gtttttcacc tgttcccaaa caggtacgcc
    14461 aagtgcaagt ccggtgaagt cgagcttgaa ggtatccagt ttgaacatca tcatcgccag
    14521 cacaacaaac agacccagcg gcaacaattt tgccagtgtc gccaccaggt taatgctggc
    14581 agcggtttgc accccgcgca ggatcaaaaa gtgaacaatc cacaataaag ccgatgcacc
    14641 gactatcgat tgccaggtat taccatcgcc aaacaggcgc aattccggcg tgtccgtaaa
    14701 aaagcttaac gcggaaaaaa cgatcaccag ataggagacg ttggcgatga ccgcgcacag
    14761 ccagtatccc catgcggaac aaaagccgat tagctcgcca aacccttcgc gggcataggt
    14821 aaagataccg ccatcaagtt cgggacgaat gcgcgtgagg atcagcatgg caaaggccag
    14881 caataaaatg ccagcgccag taatacccca gccgatgagc agtgctgccg ggctggcaac
    14941 tgccgccata ttttgcggca gactgaaaac acccgcgccc agcattgagc ttaataccag
    15001 cgcggtgagt gcgctcagtc ccagtttctt ttccatcgat atccagtgat taaaacgtga
    15061 taagaagaat aaatattagg gcaaaacgca taaaaatgga gtttgccact gaagcgcggg
    15121 attttacgaa gagatgtgtc tgcatgcaat ggtcgcgggg aaatttgtga ctaaaaatgc
    15181 gaaaggcggc atcaagccgc cttataggag taactgaata gttatttata cagatctgcg
    15241 ctgacggtca ggttattacc acgtttctgc cactggcggg tgatgtggta atacttagca
    15301 cctttcttcg cggcacgttt cgcaacctga taggagactt cggtcatgtt gccgtagttg
    15361 ccagagaatt tgatgctgtc gaacgggacc atcatcgctg ctgtcgcttt gttcagttct
    15421 tcgactttag tgccatccgg gagcgtgacg gtgtaacgcc cgccttttga tgactgggtt
    15481 tcaaagaagc gaccgacttc agaacttggt gatgcggtag tcgcaacacc cgggatctca
    15541 actttcttcg cggcttcgcc accggcagcc agagctgcac gtcctgcttc ggaatctgcc
    15601 gggatcacat ccgggctctg gacgatacgt ttcttagcat cttttttata gatgaatgca
    15661 gtaatacgct ggttgccgcc ctggttggca tcgatttgac gaacgatgta gaaagagtag
    15721 gcacctttcg cttttgccgc tttggtgatg gcatcattga cttctggctg gctacgatag
    15781 aagccctgga cggtgaccgt gtcaaacggt tcaatcagaa cagcctgatc tttcggcagt
    15841 tcgacaacac cgttaattac gcgattactt gtttcttctg ctttttcagc atcggcttta
    15901 tagaggtcag cgaccacacg ccagttaccg ctgttaccaa aatcagaagt gtcgacaaca
    15961 taaaaagagg cggcaccttc tttatctgcg cgacgagaaa cggctttcac cgcttcgcca
    16021 atagcattaa aacgaccggt aaccactaca cggtcaaaag gtttaaccgc tgccgcttgc
    16081 tccggtgtca gttctgttgc tgcgttaacg gagaatgccg tagcagaaag cagtgccgac
    16141 gccaggaggg tgttcttaag cttcataaaa ataatccttc gccttgcgca aaccaggtac
    16201 tggtattgtt attaacgaga aacgtggctg attattgcat ttaaacggtg taactgtctg
    16261 cgtcattttt catatcacat tccttaaacc aattttaatc ctgctcaaat gaccgtctat
    16321 gcttaaaaaa cagccgtatc agcatcatta ctactgaagc aactgaattg tataagttaa
    16381 tttaatgtta agtagtgatt cgtgccgggg cgatgtctcg ttttacccga ccgtcgaaga
    16441 caattatcag tctttatccg gcgttctaag gtgtttatcc cactatcacg gctgaatcgt
    16501 taatattttg cgagttcacg ccgaaatact gatttttggc gctagatcac aggcataatt
    16561 ttcagtacgt tatagggcgt ttgttactaa tttattttaa cggagtaaca tttagctcgt
    16621 acatgagcag cttgtgtggc tcctgacaca ggcaaaccat catcaataaa accgatggaa
    16681 gggaatatca tgcgaattgg cataccaaga gaacggttaa ccaatgaaac ccgtgttgca
    16741 gcaacgccaa aaacagtgga acagctgctg aaactgggtt ttaccgtcgc ggtagagagc
    16801 ggcgcgggtc aactggcaag ttttgacgat aaagcgtttg tgcaagcggg cgctgaaatt
    16861 gtagaaggga atagcgtctg gcagtcagag atcattctga aggtcaatgc gccgttagat
    16921 gatgaaattg cgttactgaa tcccgggaca acgctggtga gttttatctg gcctgcgcag
    16981 aatccggaat taatgcaaaa acttgcggaa cgtaacgtga ccgtgatggc gatggactct
    17041 gtgccgcgta tctcacgcgc acaatcgctg gacgcactaa gctcgatggc gaatatcgcc
    17101 ggttatcgcg ccattgttga agcggcacat gaatttgggc gcttctttac cgggcaaatc
    17161 actgcggccg ggaaagtgcc accggcaaaa gtgatggtga ttggtgccgg tgttgcaggt
    17221 ttggccgcca ttggcgcagc aaacagtctc ggcgcgattg tgcgtgcatt cgacacccgc
    17281 ccggaagtga aagaacaagt tcaaagtatg ggcgcggaat tcctcgagct ggattttaaa
    17341 gaggaagcgg gcagcggcga tggctatgcc aaagtgatgt cggacgcgtt catcaaagcg
    17401 gaaatggaac tctttgccgc ccaggcaaaa gaggtcgata tcattgtcac caccgcgctt
    17461 attccaggca aaccagcgcc gaagctaatt acccgtgaaa tggttgactc catgaaggcg
    17521 ggcagtgtga ttgtcgacct ggcagcccaa aacggtggca actgtgaata catcgtgccg
    17581 ggtgaaatct tcagtacgga aaatggtgtc aaagtgattg gttataccga tcttccgggc
    17641 cgtctgccga cgcaatcctc acagctttac ggtactaacc tcgttaatct gctgaaactg
    17701 ttgtgcaaag agaaagacgg caatatcact gttgattttg atgatgtggt gattcgcggc
    17761 gtgaccgtga tccgtgcggg cgaaattacc tggccggcac cgccgattca ggtatcagct
    17821 cagccgcagg cggcacaaaa agcggcaccg gaagtgaaaa ctgaggaaaa atgtgcctgc
    17881 tcaccgtggc gtaaatacgc gttgatggcg ctggcaatca tcctgttcgg ttggctgtca
    17941 agcgttgcgc cgaaagagtt tcttgggcac ttcaccgttt tcgcgctggc ctgcgttgtc
    18001 ggttattacg tggtgtggaa tgtatcgcac gcgctgcata caccgttgat gtcggttacc
    18061 aacgcgattt cagggattat tgttgtcgga gcactgttgc agattggcca gggaggctgg
    18121 gttagcttcc ttagttttat cgcggtgctt atagccagca ttaatatttt cggtggcttc
    18181 accgtgactc agcgcatgct gaaaatgttc cgcaaaaatt aaggggtaac atatgtctgg
    18241 aggattagtt acagctgcat acattgttgc cgcgatcctg tttatcttca gtctggccgg
    18301 tctttcgaaa catgaaacgt ctcgccaggg taacaacttc ggtatcgccg ggatggcgat
    18361 tgcgttaatc gcaaccattt ttggaccgga tacgggtaat gttggctgga tcttgctggc
    18421 gatggtcatt ggtggggcaa ttggtatccg gctggcgaag aaagttgaaa tgaccgaaat
    18481 gccagaactg gtggcgatcc tgcacagctt cgtgggtctg gcggcagtgc tggttggctt
    18541 taacagttat ctgcatcatg acgcgggaat ggcaccgatt ctggtcaata ttcacctgac
    18601 ggaagtgttc ctcggtatct tcatcggggc ggtaacgttc acgggctcgg tggtggcgtt
    18661 cggcaaactg tgtggcaaga tttcgtctaa accgttgatg ctgccaaacc gtcacaaaat
    18721 gaacctggcg gctctggtcg tttccttctt gctgctgatt gtatttgttc gcacggacag
    18781 cgtcggcctg caagtgctgg cattgctgat aatgaccgcc attgcgctgg tattcggctg
    18841 gcatttagtc gcctccatcg gtggtgcaga tatgccggtg gtggtgtcga tgctgaactc
    18901 gtactccggc tgggcggctg cggctgcggg ctttatgctc agcaacgacc tgctgattgt
    18961 gaccggtgcg ctggtcggtt cttcgggggc tatcctttct tacattatgt gtaaggcgat
    19021 gaaccgttcc tttatcagcg ttattgcggg tggtttcggc accgacggct cttctactgg
    19081 cgatgatcag gaagtgggtg agcaccgcga aatcaccgca gaagagacag cggaactgct
    19141 gaaaaactcc cattcagtga tcattactcc ggggtacggc atggcagtcg cgcaggcgca
    19201 atatcctgtc gctgaaatta ccgagaaact ccgcgctcgt ggtattaatg tgcgtttcgg
    19261 tatccacccg gttgcggggc gtttgcctgg acatatgaac gtattgctgg ctgaagcaaa
    19321 agtaccgtat gacatcgtgc tggaaatgga cgagatcaac gatgactttg ctgataccga
    19381 taccgtactg gtgattggtg ctaacgatac ggttaacccg gcggcgcagg atgatccgaa
    19441 gagtccgatt gctggtatgc ctgtgctgga agtgtggaaa gcgcagaacg tgattgtctt
    19501 taaacgttcg atgaacactg gctatgctgg tgtgcaaaac ccgctgttct tcaaggaaaa
    19561 cacccacatg ctgtttggtg acgccaaagc cagcgtggat gcaatcctga aagctctgta
    19621 accctgacgg cctctgcgga ggccgtcact ctttattgag atcgcttaac agaacggcga
    19681 tgctttgacc tcccgctgtt tgttcaagcg caattttgac aataattgtc aacggcacgg
    19741 aaagcagcat acccaccggt cctaacaacc atccccaaaa aatcaacgac aaaaatacca
    19801 ccaatgtcga aagccccagc ccacgtccca tgatgcgcgg ctccagaata ttgccgaaga
    19861 ccagattaat cagcagatat cccgccagca ccagcaacgc ttcgtagaag ccattaaaca
    19921 ccagtacctg agcgataggg gggattgccg cgagaactga accaatatta gggatgtaat
    19981 taagcgcaaa ggccagcaat ccccagacaa aagcgaagcg aacatcgagt gcggcgagca
    20041 tcgcccaggc gaccaggccg gtgatgatgc tgatggctgt tttcagcacc agataatgag
    20101 aaacactgtc aatcgcacgt tgaatcgccg ccatcccttc aaccggacgc gccatcattt
    20161 gctgaaattt tccgggcaat tgtggcactt cgagcagcat aaacagcacc gtcagcagca
    20221 ataaaaatat tgatgacatg gcattagata actgcgtcaa taagttggtg agcaacgtca
    20281 tcgccgcgtt cggatcaatg taatgcgcca gttggtcaac tgagacgtca atccctacgc
    20341 gttgcaacaa cggctcaaga gcttgcagcg gcgtcataat agagttgcga tattgcggta
    20401 acgtccgcgt caactcgttg agcgcggaac ccagataagc taatagcaac accatcgcca
    20461 tcacgatgat tgtcatcaaa atcgacactg ccagtacacg cggcacacgc cagcggacca
    20521 tatgttgcac cagcgggtta agaataacag caataaataa tgcgagaata aacggcacga
    20581 tgatctcggc ggcaaaacgg ataccgcaga gaataatgac cagcattccc aacataatga
    20641 cgatttttag gccattgagc gtgatgatcg gctttgccat gcttgtcccc gtttttcttc
    20701 ctttgctaat aataatgtct ttttcgctcc gtcatcataa cgaaaatcaa accgctcagg
    20761 taaagaagtg aaaatatttt gagttaattc ttaagctatg atacaaatca ggcgtgttca
    20821 actaccgagg acaattatca tctgcgatga cgagaagcaa cactgcggat aattgtaata
    20881 ttatggacaa tttgttcagg acgttatttt ctacatttac ccatcttcgt acctcatcca
    20941 ctatcttgct cgtaggtgag cagcactggc gcaacgcgct ttagtccttt cttctgccta
    21001 agcttgccct atgcgcaggg cttgaccact ttactttggt ttcgctgaat taagcgaaaa
    21061 ttaaaatagt tctcttgcag gagaaggaca atgtatattt attggatttt attaggtctg
    21121 gctattgcta cagaaattac cggtacgctg tcaatgaaat gggcgagcgt cagtgaggga
    21181 aatggcggct ttattttaat gctggtgatg atttctctgt cgtatatatt tctctctttc
    21241 gccgttaaaa aaatcgcctt aggcgtagct tatgcgctgt gggaaggtat cggtatttta
    21301 tttattacct tgtttagcgt tttgttattc gacgaaagtt tatcgctgat gaaaattgcc
    21361 gggttaacca ccctggtcgc cgggatagtg ttgatc
//
LOCUS       DAZXUK010000039        21255 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZXUK010000039 DAZXUK010000000
VERSION     DAZXUK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21255
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.039"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..793
                     /locus_tag="ACH3L8_001236"
                     /pseudo
     CDS             1..793
                     /locus_tag="ACH3L8_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(853..2151)
                     /gene="eno"
                     /locus_tag="ACH3L8_001237"
     CDS             complement(853..2151)
                     /gene="eno"
                     /locus_tag="ACH3L8_001237"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFT4084427.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="ACH3L8_001238"
     CDS             complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="ACH3L8_001238"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFT4084428.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="ACH3L8_001239"
     CDS             complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="ACH3L8_001239"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFT4084429.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4991..7225)
                     /gene="relA"
                     /locus_tag="ACH3L8_001240"
     CDS             complement(4991..7225)
                     /gene="relA"
                     /locus_tag="ACH3L8_001240"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFT4084430.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="ACH3L8_001241"
     CDS             complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="ACH3L8_001241"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFT4084431.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8631..11387
                     /gene="barA"
                     /locus_tag="ACH3L8_001242"
     CDS             8631..11387
                     /gene="barA"
                     /locus_tag="ACH3L8_001242"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFT4084432.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="ACH3L8_001243"
     CDS             complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="ACH3L8_001243"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFT4084433.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12980..14320)
                     /locus_tag="ACH3L8_001244"
     CDS             complement(12980..14320)
                     /locus_tag="ACH3L8_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFT4084434.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="ACH3L8_001245"
     CDS             complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="ACH3L8_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFT4084435.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16108..16557)
                     /locus_tag="ACH3L8_001246"
     CDS             complement(16108..16557)
                     /locus_tag="ACH3L8_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFT4084436.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16575..17357)
                     /gene="truC"
                     /locus_tag="ACH3L8_001247"
     CDS             complement(16575..17357)
                     /gene="truC"
                     /locus_tag="ACH3L8_001247"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFT4084437.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17357..17686)
                     /locus_tag="ACH3L8_001248"
     CDS             complement(17357..17686)
                     /locus_tag="ACH3L8_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFT4084438.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18308..18853)
                     /gene="syd"
                     /locus_tag="ACH3L8_001249"
     CDS             complement(18308..18853)
                     /gene="syd"
                     /locus_tag="ACH3L8_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFT4084439.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18921..19769
                     /gene="queF"
                     /locus_tag="ACH3L8_001250"
     CDS             18921..19769
                     /gene="queF"
                     /locus_tag="ACH3L8_001250"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFT4084440.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19881..21245
                     /gene="ppnN"
                     /locus_tag="ACH3L8_001251"
     CDS             19881..21245
                     /gene="ppnN"
                     /locus_tag="ACH3L8_001251"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFT4084441.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5229 a         5670 c         5068 g         5288 t
ORIGIN      
        1 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
       61 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
      121 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
      181 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
      241 actgatcgtt gttgcctagt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
      301 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
      361 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
      421 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
      481 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctggttttt
      541 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
      601 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
      661 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
      721 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcgcatc
      781 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
      841 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
      901 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
      961 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1021 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1081 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1141 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1201 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1261 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1321 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     1381 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     1441 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     1501 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     1561 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     1621 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     1681 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     1741 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     1801 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     1861 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     1921 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     1981 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2041 agcagctgcc ataccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2101 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2161 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2221 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2281 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     2341 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     2401 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     2461 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     2521 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     2581 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     2641 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     2701 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     2761 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     2821 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     2881 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     2941 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3001 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3061 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3121 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3181 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3241 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3301 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     3361 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     3421 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     3481 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     3541 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     3601 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     3661 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     3721 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     3781 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     3841 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     3901 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     3961 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4021 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4081 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4141 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4201 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4261 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4321 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     4381 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     4441 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     4501 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     4561 agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg gcgacgctct
     4621 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     4681 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     4741 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     4801 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     4861 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     4921 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     4981 ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5041 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5101 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5161 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5221 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5281 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5341 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5401 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     5461 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     5521 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5581 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     5641 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5701 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5761 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5821 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     5881 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5941 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6001 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6061 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6121 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6181 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6241 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6301 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6361 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6421 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     6481 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     6541 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6601 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6661 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     6721 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     6781 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6841 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6901 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     6961 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7021 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7081 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7141 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7201 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7261 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7321 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7381 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7441 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7501 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7561 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7621 aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7681 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7741 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7801 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     7861 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7921 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7981 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8041 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8101 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8161 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8221 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8281 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8341 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8401 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     8461 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8521 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8581 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8641 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8701 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8761 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8821 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8881 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8941 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9001 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9061 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9121 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9181 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9241 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9301 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9361 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9421 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9481 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9541 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9601 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9661 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9721 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9781 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9841 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9901 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9961 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10021 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10081 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10141 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10201 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10261 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10321 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10381 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10441 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagttaat gctgaaaaac
    10501 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    10561 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10621 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10681 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    10741 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10801 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    10861 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10921 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10981 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11041 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11101 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11161 ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11221 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11281 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11341 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11401 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11461 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11521 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11581 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11641 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    11701 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11761 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    11821 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    11881 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11941 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12001 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12061 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12121 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12181 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12241 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12301 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12361 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12421 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    12481 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12541 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    12601 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12661 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12721 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12781 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    12841 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12901 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12961 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13021 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13081 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13141 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13201 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13261 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13321 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    13381 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13441 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    13501 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13561 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    13621 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    13681 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13741 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13801 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13861 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13921 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13981 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccaaag gtatcaaaat
    14041 cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14101 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14161 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg cgggtgaagt
    14221 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14281 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    14341 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    14401 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    14461 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14521 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14581 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14641 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14701 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    14761 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    14821 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14881 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    14941 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15001 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15061 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15121 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15181 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15241 accggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15301 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15361 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15421 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15481 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15541 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15601 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15661 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    15721 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15781 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15841 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15901 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    15961 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16021 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16081 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16141 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16201 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16261 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16321 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16381 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    16441 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16501 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16561 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16621 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16681 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16741 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16801 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16861 cgtcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16921 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16981 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17041 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17101 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17161 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17221 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17281 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17341 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17401 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17461 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17521 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17581 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17641 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17701 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17761 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17821 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17881 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17941 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18001 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18061 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18121 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18181 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18241 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    18301 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18361 tcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18421 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18481 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18541 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18601 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18661 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18721 tagttgaaat aatgcacggt gaaggaacac cgtacaattc ctcacttaac ggccagcttt
    18781 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18841 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18901 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18961 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19021 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19081 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    19141 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19201 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19261 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19321 ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt gatgaccagg
    19381 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc accagcggtg
    19441 aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19501 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19561 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19621 aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc gtttacgcac
    19681 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19741 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19801 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19861 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19921 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19981 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20041 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    20101 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    20161 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccataata
    20221 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20281 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    20341 ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc
    20401 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc aggagcgatg gaagcgccga
    20461 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    20521 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc actggtcaac gaattgatca
    20581 tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20641 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20701 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    20761 actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc
    20821 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20881 cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac tggtcaatgc
    20941 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    21001 agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt gcgttctccg
    21061 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    21121 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21181 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21241 cgtaatctcc cctcc
//
LOCUS       DAZXUK010000040        20779 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZXUK010000040 DAZXUK010000000
VERSION     DAZXUK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20779
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.040"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..579
                     /locus_tag="ACH3L8_001252"
     CDS             <1..579
                     /locus_tag="ACH3L8_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFT4084442.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="ACH3L8_001253"
     CDS             597..1235
                     /locus_tag="ACH3L8_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFT4084443.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="ACH3L8_001254"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="ACH3L8_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFT4084444.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACH3L8_001255"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACH3L8_001255"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFT4084445.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="ACH3L8_001256"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="ACH3L8_001256"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFT4084446.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACH3L8_001257"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACH3L8_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFT4084447.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="ACH3L8_001258"
     CDS             complement(5552..6613)
                     /locus_tag="ACH3L8_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFT4084448.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="ACH3L8_001259"
     CDS             complement(6610..8007)
                     /locus_tag="ACH3L8_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFT4084449.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="ACH3L8_001260"
     CDS             8163..9161
                     /locus_tag="ACH3L8_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFT4084450.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACH3L8_001261"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACH3L8_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="ACH3L8_001262"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="ACH3L8_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACH3L8_001263"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACH3L8_001263"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFT4084451.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACH3L8_001264"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACH3L8_001264"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFT4084452.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..15286)
                     /locus_tag="ACH3L8_001265"
                     /pseudo
     CDS             complement(14232..15286)
                     /locus_tag="ACH3L8_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(15296..16071)
                     /locus_tag="ACH3L8_001266"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="ACH3L8_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(16089..17141)
                     /locus_tag="ACH3L8_001267"
     CDS             complement(16089..17141)
                     /locus_tag="ACH3L8_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFT4084453.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="ACH3L8_001268"
     CDS             complement(17172..18014)
                     /locus_tag="ACH3L8_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFT4084454.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="ACH3L8_001269"
     CDS             complement(18001..18882)
                     /locus_tag="ACH3L8_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFT4084455.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="ACH3L8_001270"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="ACH3L8_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(20200..>20779)
                     /gene="ycjM"
                     /locus_tag="ACH3L8_001271"
     CDS             complement(20200..>20779)
                     /gene="ycjM"
                     /locus_tag="ACH3L8_001271"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFT4084456.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5070 a         5668 c         5053 g         4988 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtttact
//
LOCUS       DAZXUK010000041        20556 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZXUK010000041 DAZXUK010000000
VERSION     DAZXUK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20556
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.041"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACH3L8_001272"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACH3L8_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFT4084457.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACH3L8_001273"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACH3L8_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFT4084458.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACH3L8_001274"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACH3L8_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFT4084459.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACH3L8_001275"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACH3L8_001275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFT4084460.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="ACH3L8_001276"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="ACH3L8_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFT4084461.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="ACH3L8_001277"
     CDS             complement(8217..9110)
                     /locus_tag="ACH3L8_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFT4084462.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACH3L8_001278"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACH3L8_001278"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFT4084463.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACH3L8_001279"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACH3L8_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFT4084464.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACH3L8_001280"
     CDS             complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACH3L8_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFT4084465.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACH3L8_001281"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACH3L8_001281"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFT4084466.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACH3L8_001282"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACH3L8_001282"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFT4084467.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="ACH3L8_001283"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="ACH3L8_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACH3L8_001284"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACH3L8_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFT4084468.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACH3L8_001285"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACH3L8_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFT4084469.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACH3L8_001286"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACH3L8_001286"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFT4084470.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..>20556)
                     /gene="ycaL"
                     /locus_tag="ACH3L8_001287"
     CDS             complement(19828..>20556)
                     /gene="ycaL"
                     /locus_tag="ACH3L8_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFT4084471.1"
                     /translation="SAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAKAIANQG
                     CAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNAWAMANGCVR
                     VYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATSGVASQLS
                     RSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASLDGGRTQ
                     SMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4923 a         5694 c         5008 g         4931 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct ctgccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcaga
//
LOCUS       DAZXUK010000042        20323 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZXUK010000042 DAZXUK010000000
VERSION     DAZXUK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20323
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.042"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="ACH3L8_001288"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="ACH3L8_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFT4084472.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACH3L8_001289"
     CDS             complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACH3L8_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HFT4084473.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFVG"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACH3L8_001290"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACH3L8_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFT4084474.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="ACH3L8_001291"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="ACH3L8_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="ACH3L8_001292"
     CDS             1631..2341
                     /locus_tag="ACH3L8_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFT4084475.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACH3L8_001293"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACH3L8_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFT4084476.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4129)
                     /locus_tag="ACH3L8_001294"
                     /pseudo
     CDS             complement(3469..4129)
                     /locus_tag="ACH3L8_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
     gene            4282..6615
                     /gene="bisC"
                     /locus_tag="ACH3L8_001295"
     CDS             4282..6615
                     /gene="bisC"
                     /locus_tag="ACH3L8_001295"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFT4084477.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6584..7024)
                     /locus_tag="ACH3L8_001296"
     CDS             complement(6584..7024)
                     /locus_tag="ACH3L8_001296"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFT4084478.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7021..7584)
                     /gene="tag"
                     /locus_tag="ACH3L8_001297"
     CDS             complement(7021..7584)
                     /gene="tag"
                     /locus_tag="ACH3L8_001297"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFT4084479.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7742..8440
                     /locus_tag="ACH3L8_001298"
     CDS             7742..8440
                     /locus_tag="ACH3L8_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFT4084480.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8669..9877
                     /locus_tag="ACH3L8_001299"
     CDS             8669..9877
                     /locus_tag="ACH3L8_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4084481.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10201..11892
                     /gene="eptB"
                     /locus_tag="ACH3L8_001300"
     CDS             10201..11892
                     /gene="eptB"
                     /locus_tag="ACH3L8_001300"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFT4084482.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11984..12060
                     /locus_tag="ACH3L8_001301"
     tRNA            11984..12060
                     /locus_tag="ACH3L8_001301"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12018..12020,aa:Pro,seq:cgg)
     gene            12804..14411
                     /gene="dppA"
                     /locus_tag="ACH3L8_001302"
     CDS             12804..14411
                     /gene="dppA"
                     /locus_tag="ACH3L8_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFT4084483.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14719..15738
                     /gene="dppB"
                     /locus_tag="ACH3L8_001303"
     CDS             14719..15738
                     /gene="dppB"
                     /locus_tag="ACH3L8_001303"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFT4084484.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15748..16674
                     /gene="dppC"
                     /locus_tag="ACH3L8_001304"
     CDS             15748..16674
                     /gene="dppC"
                     /locus_tag="ACH3L8_001304"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFT4084485.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALV
                     LVAIFGPSIGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFIN
                     IFPNCLAPLIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWV
                     VTFPGLAILLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16685..17668
                     /gene="dppD"
                     /locus_tag="ACH3L8_001305"
     CDS             16685..17668
                     /gene="dppD"
                     /locus_tag="ACH3L8_001305"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFT4084486.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17665..18669
                     /gene="dppF"
                     /locus_tag="ACH3L8_001306"
     CDS             17665..18669
                     /gene="dppF"
                     /locus_tag="ACH3L8_001306"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFT4084487.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18699..19970)
                     /locus_tag="ACH3L8_001307"
     CDS             complement(18699..19970)
                     /locus_tag="ACH3L8_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFT4084488.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4862 a         5031 c         5343 g         5087 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggatctt
     4561 attcaccaac aacataaacg cattcgtgag gcttatggtc cggcatcgat ttttgctggt
     4621 tcctacggct ggcgttcaaa cggcgtgctg cataaggcct cgacattatt acaacgctat
     4681 atggcgctgg caggcggtta taccgggcat ctgggggatt attcgaccgg tgcggcacag
     4741 gcgatcatgc cgtatgtcgt aggtggcagc gaagtttatc aacagcaaac cagttggccg
     4801 ctggtgctgg aacatagcga tgtcgtggtg ttgtggagtg ctaacccact caatacgctg
     4861 aaaattgcgt ggaatgcatc cgatgagcag gggctttctt acttttccgc actgcgtgac
     4921 agtgggaaaa agctgatctg cattgatcca atgcgatcgg aaaccgtcga tttctttggc
     4981 gataaaatgg agtgggtggc accgcacatg ggcaccgatg ttgcgctgat gctggggatc
     5041 gcccatacgc tggtggaaaa tggttggcac gacgaagcgt ttctggcgcg ttgcaccaca
     5101 ggttatgccg tcttcgcctc ttatttgctg ggcgagagtg acggaatagc gaaaaacgcc
     5161 gaatgggcgg cagagatttg tggtgttggc gcagcgaaaa tccgcgagct ggcggctatt
     5221 ttccaccaaa ataccaccat gctgatggca ggctggggaa tgcagcgcca acagtttggt
     5281 gagcaaaaac actggatgat cgtcacgctg gcagcaatgt tggggcaaat cggcacaccc
     5341 ggcggcggtt ttggtctttc ttaccatttt gccaatggtg gtaaccccac gcggcgttct
     5401 gcggtgctct cttccatgca gggcagcttg ccgggtggct gcgatgcggt ggataaaatc
     5461 cctgttgccc gcattgttga agcactggaa aaccctggtg gcgcatatca acacaacggt
     5521 atgaaccgac atttcccgga tattcgtttt atctggtggg cgggcggtgc caactttact
     5581 catcatcagg ataccaatcg tctgatccgt gcctggcaaa aaccggagct ggtggtgatc
     5641 tctgaatgct tctggacggc ggcggcgaaa cacgcggata ttgttctgcc tgcgactacc
     5701 tcttttgagc gtaatgatct caccatgacc ggtgattaca gtaatcagca tctggtgccg
     5761 atgaagcaag tggtgccgcc acgctatgaa gcgcgtaatg attttgatgt ttttgccgag
     5821 ttgagtgaac gttgggagaa gggcggctat gcgcggttta cggaaggaaa aagtcagctg
     5881 caatggctgg aaacgtttta taacgttgcc cggcagcgcg gggcaagcca gcaggttgaa
     5941 ttgccgccat ttgctgagtt ctggcaagcc aaccagttaa ttgagatgcc ggaaaacccg
     6001 gacagcgagc ggtttattcg cttcgctgat ttttgccgcg atccgctggc gcatccgtta
     6061 aaaaccgcca gcggcaagat tgaaatcttc tcacagcgta ttgccgatta cggttacccg
     6121 gattgccctg ggcatccaat gtggctggag ccggacgaat ggcagggcaa tgccgaacca
     6181 gaacagttgc aggtactttc tgcccatccg gcgcaccgcc tgcacagcca gctgaattac
     6241 agttctctgc gcgaattgta cgcggtagca aaccgtgagc ctgtcaccat tcatcctgac
     6301 gatgctcagg cgcgcggcat aaccgaaggg gatatggtgc gggtgtggaa ctcccgtggg
     6361 cagatccttg ccggagcggt cattagcgag ggaattaaac ctggcgtgat ttgcattcac
     6421 gaaggggcat ggccggatct ggatttaacc gctgacggta tttgtaaaaa cggcgcagtg
     6481 aacgtgctga ccaaagatct ccccagctcg cggctgggga atggctgtgc gggtaatacg
     6541 acgctggcat ggctggaaaa atacaacggt ccggaactga cacttacagc gtttgaacca
     6601 ccggccagct cataatccag gtgggtagtt gggtttcatc ctgccatgcg caatcgacaa
     6661 tgtgaaaacc ctgcgcacgg taaaaatcta tcgccggttg atttttttga taaacctcca
     6721 gcatcagatg gggatagcgc tgctgcacat actgcatcag cgccttacca ataccgcgtc
     6781 tgacggcctt cggtgcgaca aacattgctg ccagaaatcg gccttccata atgctgacaa
     6841 aaccgagaag cttaccgtcc tcttcccaga cccagttttg cgcgttggca agataggcat
     6901 cccgcaccag cggaatgcag tcacgccagt aattcgcttt tataaaggga tgcccccagg
     6961 ttgtactttc cagccacagt tcgaggatcg cggggagttc tgaacgttgc gcttcccgaa
     7021 tcatggttta tttcccagat agcaacagca gccaaccaca tgatcattca ccagcccaca
     7081 tgcctgcata aaggagtaac agattgtggt gccgacaaac ttaaaaccac gttttttcag
     7141 tgccttagat agggcgtcgg aggcggacgt agatgtggga atttcgctca acgttgtggc
     7201 ttgtgtcacc tgtggctgat gatttacaaa cgaccagaca aagtcgggaa acggttcgcc
     7261 gttctgttcc atttgcaggt acgcccgcgc attaccaata attgcctgaa ttttccctcg
     7321 atggcggata atcccggcgt cctgtaccag tctttcgaca tcctcttcct gcattgctgc
     7381 gaccttcacc ggatcgaact gatgaaagta ggcgcgatag ttttcgcgtt ttttgaggac
     7441 ggtgatccac gataacccag cctgctgccc ttcaaagcag atcatttcga acagtttttt
     7501 actgtcagtt tcaggcacgc cccactcatt atcatggtag gcaatataaa gcgggccctg
     7561 actcacccag ccgcaacgtt ccatactttc cctcgctatg tacccgatcg taaaaatttc
     7621 agccgctttt cttgacgcat cggtgaaaaa gtcaatattt aatacagggc tactatgccc
     7681 gatatccttc atacaatgac aataatattg ccgaattcgg ctcttccctg gagtcgtgtt
     7741 aatgatcata aaaaaaagcg gtggtcgctg gcagctaagc ctgctggcga gcgtggtaat
     7801 cagtgccttt tttctcaaca cagcttacgc ctggcaacaa gaatatatcg ttgatacgca
     7861 acccggacat tccacagagc gttacacctg ggatagtgat catcaacctg attacaacga
     7921 tattttgtcg caacgtattc aaagtagcca aagggcgctg ggactggaag tcaatctggc
     7981 ggaagaaact cctgtggatg tgaccagcag tatgagtatg ggctggaatt ttcctttata
     8041 tgaacaggtt acaaccggcc cggtcgcggc attacattac gatggcacaa ccacctcgat
     8101 gtataacgag tttggcgaca gtactaccac gctgaccgat ccgttatggc atgccagcgt
     8161 gagtagctta ggctggcgtg ttgactcccg gcttggcgat ctccgaccct gggcgcaaat
     8221 cagctataac cagcaatttg gcgagaatat ctggaaggcg caatcaggcc tgagccggat
     8281 gacggcgaca aaccagaacg gcaactggct ggatgtcacc gtaggcgctg atatgttgct
     8341 caatcaaaat attgccgcct atgctgcgct aactcaggca gaaaatacca ctaataatag
     8401 cgactatctg tatacgatgg gggttagcgc cagattttaa cgtaacagtc acaattgaaa
     8461 ccattgaata acaacagttg tggcgatagt gggtgctaac ttaccaaata ataaatttgg
     8521 tgaataattg tcgcgttatt cattcctgaa ctaaggcatt tcattccgtt ctgatggcat
     8581 ttcatgccgt ttttccccaa gcataaaatg cacttcgtta tggttgtcgg cagagatttt
     8641 tcctttttat tactgcagga atactgccat gacaccttca aattatcagc gtacccgctg
     8701 gctgacactc atcggtacta tcattaccca gtttgcgctg gggtcggttt atacccggag
     8761 cctgtttaat ggcgcgcttt ctgccaagct ggatgcgccg gtaagccagg tcgctttctc
     8821 tttcggcttg ttaagtctgg ggctggcaat ttcgtcttct gttgcgggca aattgcagga
     8881 acgttttggc gttaaacgcg tcaccgtggc ttccggcatt ttgctgggat taggcttctt
     8941 cctgaccgcg cattccaaca acctgatgat gctgtggtta agcgccggtg tgctggtcgg
     9001 tctggcggat ggcgcgggtt acctgctgac gctctctaac tgcgtgaagt ggttcccgga
     9061 gcgtaaaggt ctgatctccg cgttcgctat cggttcttat ggtctgggta gcctgggttt
     9121 caaatttatc gacacgcagc tgctcgaaac ggtgggtctg gaaaaaacct ttgtgatttg
     9181 gggagcgatt gcgctggtga tgattgtttt cggcgcaacg ttaatgaaag acgcaccaaa
     9241 acaggaagtg aaaaccagca atggtgtggt ggagaaagat tacacgctgg cagagtcgat
     9301 gcgtaaaccg cagtactgga tgttagcggt aatgttcctg actgcctgca tgagcggcct
     9361 gtacgtgatt ggggtagcga aagatatcgc ccaaagtctg gcacaccttg atgtggtttc
     9421 cgcagccaat gcagtaacgg ttatttccat cgctaacctt tcaggtcgtc tggtgctggg
     9481 cattctgtct gacaaaattg cccgtatccg tgttatcacc attggtcagg tgatttcgct
     9541 ggtgggtatg gcggccctgc tgtttgcacc attaaatgca gtgacgttct ttgcagcgat
     9601 tgcctgcgtg gcatttaact ttggcggcac tattaccgtc tttccatcac tggtcagtga
     9661 gttcttcggc ctcaataacc tggcgaaaaa ctacggtgtg atttacctcg gtttcggtat
     9721 cggtagcatt tttggttcga ttatcgcctc actgtttggc ggtttctatg tgaccttcta
     9781 cgtgattttc gccctgctga ttctgtcatt ggcgctttct acgacgattc gtcagccaga
     9841 gcagaaaatg ttgcgtgagg cgcatggctc cctttaatgc tcagccgcga gctactgggc
     9901 ttcagcttcc gtgtgcatca aacgcatcaa cgaaatgtat aaacaatgac tcgtaaaatt
     9961 tgggccagtt aactggccct ttttactgct ttttgtaacg ctaaagtctc ttttcaaact
    10021 tgcatttttg taaatttgtg cttcatgcac actctttccc cacacttttt cgctttgctg
    10081 tggtctactt attcgcgcgt gtagatttta cttatctgac tacctccgca cttttcccct
    10141 gccgggcctg aaaagccgct aagcagggtg ttatcacctg tttgtccagg gtttgtttgc
    10201 atgagataca tcaaatcgat tacacagcag aagctgagct ttttgcttgc aatctatatt
    10261 ggccttttta tgaatggcgc ggttttttat cgccgcttcg gcagctatgc gcacgatttt
    10321 accgtctgga aaggcgtttc tgctgttgtt gaactggccg ccaccgtact ggtgaccttc
    10381 tttttactac gtcttctatc gctgtttggc cgccgcagct ggcgtattct ggcgtcgctg
    10441 gtggtgctct tttccgcagg tgccagctat tacatgacct tccttaatgt ggtcattggt
    10501 tatggcatca tcgcttccgt catgaccacc gatatcgacc tgtcaaaaga agttgttggt
    10561 ctgaacttta ttctctggtt aatcgccgtt agtgcattgc ctcttatcct tatctggaat
    10621 aaccgctgtc gctacacctt gctccgacaa ctgcgaaccc cagggcagcg tattcgcagc
    10681 ctggtggtcg tcgtactggc gggtattatg gtttgggcac cgattcgttt gctggatatc
    10741 cagcagaaga aagtggagag ggcgaccggc gttgatttgc cgagttatgg cggtgtcgta
    10801 gcgaactctt atctgccatc aaactggctt tctgcgttgg ggctgtatgc ctgggcgcgg
    10861 gtcgatgaat cttccgataa taattcattg cttaatccgg cgaagaagtt tacataccag
    10921 gctccacaaa acgttgatga tacttatgtc gtgtttatca tcggtgaaac cacgcgttgg
    10981 gaccatatgg gtattttcgg ctatgagcgt aataccacgc cgaaactggc ccaggagaaa
    11041 aatctggcgg cgttccgtgg ttactcctgt gataccgcaa ccaaactctc actgcgttgc
    11101 atgtttgtac gtcagggggg cgcggaagat aatccgcagc gcacattaaa agaacagaac
    11161 attttcgcgg ttctgaagca gttaggattc agttctgacc tctacgctat gcagagcgaa
    11221 atgtggttct acagcaacac gatggcagac aacattgctt atcgtgagca gattggtgcg
    11281 gagccacgta atcgtggcaa gccggtagat gatatgttgc tggtagacga aatgcagcaa
    11341 tcgctagggc gcaacccgga tggtaagcat ctgatcattc tgcataccaa aggttcgcat
    11401 tttaactaca cccagcgtta tccgcgtagc ttcgcgcagt ggaagccgga atgtattggt
    11461 gttgatagcg gctgtaccaa agcgcagatg atcaactcct atgacaactc ggtgacctat
    11521 gtggatcact ttatctccag cgtgattgat caggttcgcg ataagaaagc gattgtgttc
    11581 tacgcagctg accacggtga gtcaattaat gaacgcgagc acctgcacgg cacgccgcgt
    11641 gaactggcac cgccggagca gttccgcgta ccgatgatgg tctggatgtc agataaatat
    11701 ctggaaaatc cggccaatgc gcaggcgttt gcgcagctga aaaaagaagc cgacatgaaa
    11761 gtgccacgcc gtcacgtaga gctgtacgac accatcatgg gttgtcttgg ctatacttca
    11821 ccggatggtg gaattaacga aaacaacaac tggtgtcaca tcccgcagac aaaagtggca
    11881 gcggctaact aagcggcctg ctgactttct cgccgatcaa aaggcatttt gctattaagg
    11941 gattgacgag ggcgtatctg cgcagtaaga tgcgccccgc attcggtgat tggcgcagcc
    12001 tggtagcgca cttcgttcgg gacgaagggg tcggaggttc gaatcctcta tcaccgacca
    12061 aattcgaaaa gcctgctcaa cgagcaggtt tttttgcatt tgtagctcat gaggatgaga
    12121 acctccgggg gcagggaggc tcgactcgac gtaggcctga taagacgcgc cagcgtcgca
    12181 tcaggcaacg gcaccgcact tattgtcgga tgcggcgcga ccgccttatt cgaccaacac
    12241 accacacatt ttcctgccaa ctttgcgccc catatctcac tatccattaa cggatttgtg
    12301 acagaaacca ttgtcttttt ttatatttgc ttaaatcttt tttgcgttac ttatggctgg
    12361 cgctggcatt ttccgctgtg cgttttagcg ttcatggtat taatcgctca gatatccacc
    12421 ttttcctcag atttttttca ttaaatgcat aaaacttaat caccaaatgt tgctaaatat
    12481 taagctgatt gttctggtta tgttggagta gcacaaattt ctctgctgca aatagctgtt
    12541 tgaagttttt ttaatctttg tttgtggatt ctcaccgttg gtgtaaatcc cggttggctg
    12601 tattgacgtt ttcacattct gttgacagat tgtaggtcac gaggggcatt ttatggagga
    12661 tccgcactgt tacactgatg ttaattagta cggcatcccc acctcataac gttgacccga
    12721 ccgggcaaaa aacaaaaaag gtcaggcagc gacaacccac tgcaaagggt taaaacaaca
    12781 aacatcacaa ttggagcaga ataatgcgta tttccttgaa aaagtcaggg atgctgaagc
    12841 ttggtctcag cctggtggct atgaccgtcg cagcaagtgt tcaggctaaa actctggttt
    12901 attgctcaga aggatctccg gaagggttta acccgcagct gtttacctcc ggcaccacct
    12961 atgacgcctc ttccgtcccg ctttataacc gcctggttga atttaaaatc ggcaccaccg
    13021 aagtgatccc gggcctcgct gaaaagtggg aagtcagcga agacggtaaa acctatacct
    13081 tccatctgcg taaaggtgtg aagtggcacg acaataaaga attcaaaccg acgcgcgaaa
    13141 tgaacgccga tgacgtggtg ttctcgttcg atcgtcagaa aaacgcgcaa aacccgtacc
    13201 ataaagtttc tggcggcagc tacgaatact tcgaaggtat gggcttgccg gagctgatca
    13261 gcgaagtgaa aaaggtggac gataacaccg ttcagtttgt gctgactcga ccggaagcgc
    13321 cgttcctcgc tgacctggca atggacttcg cctctattct gtcaaaagaa tatgctgacg
    13381 cgatgatgaa agccggtaca ccggaaaaac tggacctcaa cccaatcgga accggtccgt
    13441 tccagttaca gcagtaccaa aaagattccc gtatccgcta caaagcgttt gatggctact
    13501 ggggcaccaa accgcagatc gatacgctgg tcttctctat tacccctgat gcttccgtgc
    13561 gttacgcgaa attgcagaag aatgaatgcc aggtgatgcc gtacccgaac ccggcagaca
    13621 tcgctcgcat gaagcaggat aaatccatca acctgatgga aatgccgggg ctgaacgtcg
    13681 gctacctctc gtataacgtg cagaaaaaac cgctggatga cgtgaaagtt cgccaagcac
    13741 taacctacgc ggtgaacaaa gacgcgatca tcaaagcggt ttatcagggc gcgggtgtat
    13801 cagcgaaaaa cctgatcccg ccaaccatgt ggggctataa cgacgacgtt caggattaca
    13861 cctacgatcc tgaaaaagcg aaagccttgc tgaaagaagc gggtctggaa aaaggtttct
    13921 ccatcgacct gtgggcgatg ccggtacaac gtccgtataa cccgaacgct cgccgcatgg
    13981 cggagatgat tcaggcagac tgggcgaaag tgggcgtgca ggccaaaatc gtcacctacg
    14041 aatggggtga gtacctcaag cgtgcgaaag atggcgaaca tcagacggta atgatgggct
    14101 ggactggcga taacggggac ccggataact tcttcgccac cctgttcagc tgcgccgcct
    14161 ctgaacaagg ttctaactac tcaaaatggt gctacaaacc gtttgaagat ctgattcaac
    14221 cggcgcgtgc taccgacgac cataacaaac gcgttgaact gtacaagcaa gcgcaggtgg
    14281 tgatgcacga tcaggctccg gcactgatca tcgctcactc caccgtgttt gaaccggtac
    14341 gcaaagaagt caaaggctat gtggttgatc cattaggcaa acatcacttc gaaaacgtct
    14401 ctatcgaata attaaaagcc atacaagact gatggcaaag gcaaaactgc ctgatgtgct
    14461 ccgcttatca ggcctacgaa aattctgcaa tgtattgaat ttgcacgatt ttgtaggccg
    14521 gataaggcgt taacgccgca tccggcataa acaaagcgca ctttgtcaac aatctgtata
    14581 cccggtggcg ctgtgcctct gcgtgcagcg ccatccggca gcaatacttc attctctgtc
    14641 cgacaagggc gggaattgat ttgtgagcaa tacagacacg cagttccagg ctgcgagtca
    14701 ctatagagaa tccgggttat gttgcagttt attctccgac gtttgggact cgtcatcccc
    14761 acgtttatcg gtattaccct tctcacattt gcctttgtcc acatgatccc gggcgatccg
    14821 gtgatgatca tggcaggcga acgtgggatc tcccctgagc gtcacgtgca gctgctggct
    14881 gaactcggct tagataaacc gatgtggcag cagtatctcc attacatttg gggcgtaatg
    14941 cacggcgatc taggcatttc aatgaaaagc cgaattccag tatgggaaga gttcgtgccg
    15001 cgctttcagg ccacgctgga acttggcgtc tgcgcgatga tttttgctac ggcagtcggt
    15061 attccggttg gtgtgctggc tgcggttaaa cgcggttcca ttttcgatca cactgcagtt
    15121 ggcctggcgc tgaccggcta ctcgatgccg atcttctggt ggggcatgat gctgatcatg
    15181 ctggtttcgg tgcactggaa cctgacgccc gtctccggtc gcgtgagcga tatggtgttc
    15241 ctcgatgact ccaatccgtt aaccggtttt atgctaatcg acaccgccat ctggggtgaa
    15301 gacggcaact ttatcgatgc cgtcgcccat atgatcttgc ccgccattgt gctgggtact
    15361 attccgctag cggtcattgt gcgtatgaca cgctcctcga tgctggaagt gctgggcgag
    15421 gattacatcc gcaccgcgcg cgccaaaggg ctaacccgca tgcgggtgat tatcgtccat
    15481 gcgctgcgta acgcgatgct gccggtagtg accgttatcg gcctccaggt gggaacattg
    15541 ctggcggggg cgattctgac cgaaaccatc ttctcgtggc ccggtctggg gcgctggttg
    15601 attgacgcac tgcaacgccg cgactatccg gtagtgcagg gcggcgtatt gctggtggcg
    15661 acgatgatta tcctcgtcaa cttgctggtc gatctgctgt acggcgtggt gaacccgcgt
    15721 attcgtcata agaagtaagg ggacatcatg tcacaggtta ctgaaaataa agtgattagc
    15781 gcaccggtgc cgatgacccc gttacaggag ttctggcact attttaaacg caacaaaggc
    15841 gcggtcgtcg ggctggttta cgtcgccatc gtgctgttca tcgcgatctt tgccaactgg
    15901 attgcgcctt ataacccggc ggatcagttc cgcgatgcgc tgctcgcccc gccagcctgg
    15961 caggaaggcg gcagtatggc acacttgctg ggcaccgatg acgtcggtcg tgatgtgctg
    16021 tcgcggctga tgtacggcgc gcgtttatcg ctgctggttg gctgtctggt ggtggtgttg
    16081 tcgctgatta tgggcgttat tctcggcctg atcgccggtt acttcggcgg cctgatcgcc
    16141 ggttacttcg gcggcctggt tgataacatt atcatgcgcg tggtcgatat catgctggcg
    16201 ctgccaagtc tgctgttggc actggtgctg gtggcgattt tcggcccgtc gattggtaac
    16261 gccgcgctgg cgctgacctt cgttgccttg ccgcactatg tgcgcttaac ccgcgccgcc
    16321 gtgctggtgg aggtgaatcg cgattacgtc accgcctctc gcgtggcagg tgccggggcg
    16381 atgcgccaga tgtttattaa catcttcccg aactgccttg cgccgctgat tgttcaggcg
    16441 tcgctcggtt tctctaacgc cattctcgat atggctgctc ttggtttcct cggcatgggg
    16501 gcacagccgc caacgcctga gtggggcacc atgctctccg acgtgttgca gttcgcgcaa
    16561 agcgcctggt gggtcgtgac cttccctggt ctggcgatcc tgttgacggt gctggcattt
    16621 aacctgatgg gtgacggtct gcgtgacgcg ctcgatccca aactaaagca gtaagaggtt
    16681 cgagatggcg ttattaaatg tagataaatt atcggtgcat ttcggcgacg aaagcgcacc
    16741 gttccgcgcc gtagaccgca tcagctacag cgtaaaacag ggcgaagtgg tcgggattgt
    16801 gggtgagtcc ggctccggta agtcggtcag ttcactggcg attatggggc tgattgatta
    16861 tccgggccgc gtaatggcag aaaaactgga gtttaacggc caggatttgc agcgtatctc
    16921 agaaaaagag cgccgcaacc tggtgggtgc cgaagtggcg atgatcttcc aggacccgat
    16981 gaccagcctt aacccgtgct acaccgtggg tttccagatt atggaagcga ttaaagtgca
    17041 tcagggcggc aacaaaagta cccgccgtca gcgggcgatt gacctgctga atcaggtcgg
    17101 tattcccgat ccggcttcgc gtctggatgt ttacccgcat cagctttccg gcggcatgag
    17161 ccagcgcgtg atgatcgcca tggcgattgc ctgtcggcca aaactgctga ttgccgatga
    17221 accgaccacc gcgctggacg tgaccattca ggcgcaaatc atcgaactac tgctggagct
    17281 acagcagaaa gagaacatgg cgctggtgtt aattacccat gacctggcgc tggtggcgga
    17341 agctgcgcat aaaatcattg tgatgtatgc aggtcaggta gtagaaacgg gcgatgcaca
    17401 cgccatcttc catgcgccgc gtcacccgta tactcaggca ttgctgcgtg cgctgccaga
    17461 atttgctcag gacaaagaac gtctggcgtc gttgccaggt gttgttcccg gcaagtatga
    17521 ccgcccgaac ggctgcctgc ttaacccgcg ctgcccctat gccactgaca gatgtcgcgc
    17581 tgaagaaccg gcgctgaata tgctcgctga tgggcgtcag tccaaatgcc attacccact
    17641 tgatgatgcc gggaggccca cactatgagt acgcaagagg ccacctcgca acaaccgctg
    17701 ttgcaggcta tcgacctgaa aaaacattat ccggtgaaga aaggcatgtt cgtgccggaa
    17761 cgtctggtga aggcgctgga cggcgtttcg tttaaccttg aacgtggcaa aacgctggca
    17821 gtagtgggcg aatctggctg cggtaaatcg accctcggtc ggttgctgac gatgatcgaa
    17881 acgcccaccg gcggcgagct gtattatcag gggcaggatc tgctcaagca cgatccgcag
    17941 gcgcagaagc tgcgtcggca gaaaatccag atcgtcttcc agaatcccta cggttcgctg
    18001 aatccgcgta agaaagttgg gcaaattctc gaagagccgc tgcttattaa caccagctta
    18061 agcaaagaac agcgtcggga aaaagccctg tcgatgatgg caaaagtcgg cctgaaaacc
    18121 gagcactatg accgctatcc gcacatgttt tccggcggac aacgtcagcg tatcgctatt
    18181 gcccgtggtc tgatgctcga cccggatgtg gtgattgccg atgagccggt ttctgcgctg
    18241 gacgtctcag tgcgtgcgca ggtgctgaat ctgatgatgg atttgcagca ggagttgggg
    18301 ctttcttatg tctttatctc ccacgacctg tcggttgtgg agcacattgc tgatgaagtg
    18361 atggtgatgt acctaggccg ctgcgtggag aaaggaacga aggaccaaat cttcaataac
    18421 ccgcgccatc cgtacaccca ggcgctgctc tccgcgacgc cacgcctgaa cccggacgat
    18481 cgtcgtgaac gcatcaaact caccggtgaa ctgccaagcc cgctgaatcc tccgccgggt
    18541 tgcgccttca acgcccgctg tcgtcggcgc ttcggcccct gcacccagtt gcagccgcag
    18601 ctaaaagact acggaggtca actggtagct tgttttgctg ttgatcagga tgaaaatccg
    18661 cagcgttaac tcacaaaacc ggggccatgc gctccggttt actcaatcag cttcaacagt
    18721 ggcgacaagc atagcaaaat gccgtaaagc agaatcagcc aggctttaaa tccgcgaagt
    18781 ttttccagtt gtgcgacttt atagatcagg aaaaacggaa tgaggcacga cacaatgcca
    18841 tataacgggc ttcccaactg aaagaacacc agcactgata cacgaaacga aacccaaatc
    18901 gtcaacgtaa tgacgataaa agcgcagatc gccagagtca gcatgcgtga gttaattttc
    18961 ttggtatcaa taattcggct taacagattg agaataatgc ctttaatagc ctcgtggaaa
    19021 ccgagataaa tgccaaagaa tgcggtcagt acggcaaaga tattcagcac cgtagaggtg
    19081 atatgaatga tatgtccagg gatcacctgc gcggccagtg ccagtgccga gatattttgt
    19141 tcaaaggcag aaacggcttc ttcgtggcta attgagaagg taaacgaaaa ggcaaaaaac
    19201 aggatcaccg cgatgagcgt gatataacta attcggtggg tacgcagcgc gagccgggtt
    19261 gccagtactt tatctgcttc ccgtttacga taggcaatat tcattgggtt aagtacctga
    19321 ataaataccg cagaaaagaa gcaaaatgga atggtaagca ggacatcgcg gaaaaagacg
    19381 gaggcttgcg ggaaggcggt tatattggcg aaattccagt gcgggatcat cgcaaaaccg
    19441 aacacgacaa taatccctac tttgaccacc accattggcc cagaaatctt aaacagtaat
    19501 cgttcaccac cagacgcaat cgctatcagt acggcgaaaa tagcgacttt ataaagtaga
    19561 gattgtgaaa gatcggcatc ggttaaaccg aaggttttca ggtacgaggc gctgtcgaaa
    19621 accacggaga gagagtagat aaaaatcccg tggataatca tcaaaaagta gataaccccg
    19681 aggaaaattc cccagttctt ccccaggtaa tgactgataa tatcggtgta gtcattacag
    19741 gaatcgcttt cagaaagggt ttttaaataa atgtcctgca ctacccaggt gacaggataa
    19801 gcaatgatcg ctgcggtaat aaatacccaa attcccttca agccaatttg tactggcatc
    19861 agcacggttc cggcaccaat cgccatgcct atgcataata aaacccagcc gaagtcgtag
    19921 cgtgtaaacg gcaatttctg attgtgtttc gatagtgtgt tgtgctgcat aaagtcctgt
    19981 ccagaaagaa gtcggcctcg caaaaatgaa taaacccagt ttctcttgcg caacaggtgg
    20041 agaagtcgaa tacgcagtat gtaaaaaatg aggcaacaga ttcactgggg aggtcgcgag
    20101 gaggcgcaat tctacaaaat tgcgcatttc actaccagtc tggagtcagc ccggatatga
    20161 actgcaacta ttgcaaataa agaaattaag ctggaaggca tcgcgcaatg aggaagattg
    20221 ccgggtgctg acagtaaaaa gcaatcgtag aatcagcgtc aagccggaag gattccggtg
    20281 aggcgcaatg ttgcgggggc ttaatccctg gtggcattgg ttg
//
LOCUS       DAZXUK010000043        20264 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZXUK010000043 DAZXUK010000000
VERSION     DAZXUK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20264
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.043"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            26..739
                     /locus_tag="ACH3L8_001308"
     CDS             26..739
                     /locus_tag="ACH3L8_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFT4084489.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(775..1731)
                     /locus_tag="ACH3L8_001309"
     CDS             complement(775..1731)
                     /locus_tag="ACH3L8_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFT4084490.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1731..2972)
                     /gene="clsB"
                     /locus_tag="ACH3L8_001310"
     CDS             complement(1731..2972)
                     /gene="clsB"
                     /locus_tag="ACH3L8_001310"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFT4084491.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPRQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(2969..3730)
                     /locus_tag="ACH3L8_001311"
     CDS             complement(2969..3730)
                     /locus_tag="ACH3L8_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFT4084492.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3863..4273
                     /locus_tag="ACH3L8_001312"
     CDS             3863..4273
                     /locus_tag="ACH3L8_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFT4084493.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(4235..5341)
                     /locus_tag="ACH3L8_001313"
     CDS             complement(4235..5341)
                     /locus_tag="ACH3L8_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4084494.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5352..6485)
                     /locus_tag="ACH3L8_001314"
     CDS             complement(5352..6485)
                     /locus_tag="ACH3L8_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4084495.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6478..8214)
                     /locus_tag="ACH3L8_001315"
     CDS             complement(6478..8214)
                     /locus_tag="ACH3L8_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFT4084496.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(8207..9202)
                     /gene="hlyD"
                     /locus_tag="ACH3L8_001316"
     CDS             complement(8207..9202)
                     /gene="hlyD"
                     /locus_tag="ACH3L8_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFT4084497.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(9205..9876)
                     /gene="cecR"
                     /locus_tag="ACH3L8_001317"
     CDS             complement(9205..9876)
                     /gene="cecR"
                     /locus_tag="ACH3L8_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFT4084498.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            10105..11325
                     /gene="rhlE"
                     /locus_tag="ACH3L8_001318"
     CDS             10105..11325
                     /gene="rhlE"
                     /locus_tag="ACH3L8_001318"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFT4084499.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSVAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGIRLMNKRKTA"
     gene            complement(11319..11417)
                     /locus_tag="ACH3L8_001319"
                     /pseudo
     CDS             complement(11319..11417)
                     /locus_tag="ACH3L8_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11499..11981)
                     /gene="ybiA"
                     /locus_tag="ACH3L8_001320"
     CDS             complement(11499..11981)
                     /gene="ybiA"
                     /locus_tag="ACH3L8_001320"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFT4084500.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            12101..14251
                     /gene="dinG"
                     /locus_tag="ACH3L8_001321"
     CDS             12101..14251
                     /gene="dinG"
                     /locus_tag="ACH3L8_001321"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFT4084501.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14279..15241
                     /gene="ybiB"
                     /locus_tag="ACH3L8_001322"
     CDS             14279..15241
                     /gene="ybiB"
                     /locus_tag="ACH3L8_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFT4084502.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15382..16467
                     /gene="hcxB"
                     /locus_tag="ACH3L8_001323"
     CDS             15382..16467
                     /gene="hcxB"
                     /locus_tag="ACH3L8_001323"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFT4084503.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            16534..16593
                     /gene="ybiE"
                     /locus_tag="ACH3L8_001324"
     CDS             16534..16593
                     /gene="ybiE"
                     /locus_tag="ACH3L8_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFT4084504.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(16696..16956)
                     /gene="ybiJ"
                     /locus_tag="ACH3L8_001325"
     CDS             complement(16696..16956)
                     /gene="ybiJ"
                     /locus_tag="ACH3L8_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFT4084505.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17221..17487)
                     /gene="ybiI"
                     /locus_tag="ACH3L8_001326"
     CDS             complement(17221..17487)
                     /gene="ybiI"
                     /locus_tag="ACH3L8_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFT4084506.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPSVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(17561..18238)
                     /gene="ybiX"
                     /locus_tag="ACH3L8_001327"
     CDS             complement(17561..18238)
                     /gene="ybiX"
                     /locus_tag="ACH3L8_001327"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFT4084507.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18280..20264)
                     /locus_tag="ACH3L8_001328"
                     /pseudo
     CDS             complement(18280..20264)
                     /locus_tag="ACH3L8_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4714 a         5297 c         5453 g         4800 t
ORIGIN      
        1 gttttttcta aggacaacta aaagaatgga atcatactcg caaaacagca ataaattaga
       61 ttttcagcac gaggccagga tattaaacgg tatatggctc attaccgctt taggtttggt
      121 ggcaaccgca ggactagcct ggggagctaa gtatctcgaa attacggcaa ccaaatatga
      181 ttcaccacca atgtatgtcg ccatagggtt attattactt tgtatgtatg gcttaagtaa
      241 ggatatcaac aagataaatg ccgccatcgc gggcgtaata tatctgtttt tactctcttt
      301 ggtggcgatt gtcgttgcaa gtttagttcc tgtatatgcc attatcatcg tgttcagcac
      361 tgcgggcgcg atgtttttaa tcagtatgct ggccggttta ttatttaatg ttgatcctgg
      421 ttctcaccgt tttatcatta tgatgacgtt gacagggttg gccctggtaa tcatcgtgaa
      481 tgcggcatta atgagtgaac ggcccatttg ggtaataagt tgcttaatga ttgtgttatg
      541 gccaggcatt atctcgcatg gacgaaataa gctccttgaa ctggcaggaa aatgccatag
      601 tgaagagttg tggagtccgg ttcgttgcgc ttttacaggt gcattaacac tctattacta
      661 ttttatcggc ttctttggga tacttgccgc gatagctata acgcttgtct ggcaaaggca
      721 tacgcgtttt tttcattagt cgtaaatgca gatactaaag aacttaacta aacttcacat
      781 cgccgcttca tttttcgccc gtagcttctt cgcttggctt tccagtagca ggtaacagat
      841 cagcgccagc agcagcggga taaagtaata caggacgcga taagcgagca gggcggcgat
      901 aatgctccct ttcgaggtat gctccccagc cagtagtgcg ataaacaccg cttccagcac
      961 accgatcccc gccggaatat gcacgatgac gccagcaata ctactaacca gtaacacgcc
     1021 cagtacaaag aaatagttca cgctttgacc aagtaacagc cagataatcg cccccattac
     1081 catccagtta acgctggaaa tcagcatctg agcgagggcg aatttccatg aaggcagcac
     1141 cagcttttgc cctttgatgg tcatatggcg gtgcttcgcg aaagcgcaaa accacaaata
     1201 aaccgcgata atcatcagta agccaatgcc gagaatacgc agcgtagttt gatcgacata
     1261 ccagtaatcc ggcaactcca ccacgcctgc ggtaaagata atccctgcca gtaaaatata
     1321 gcccagccag ttggtggtaa tactgagcga ggaaatccgc gtaatagtgc tgcccggtaa
     1381 ccctagtcgg gagtacaaac gatagcgcat accaatgccg ccgacccagg tactgagcgt
     1441 caggttgaag gcgtagcaga taaacgacac cagcatcacc tggcgcttcg ccagtttgtg
     1501 accgcagtaa aagcgggcga gcaggtcata gcagccgtaa atcagatagc tgacgaccac
     1561 cagcccgacc gcactaagca gcgcaacgcg attgtagtcg cggatgacct tccagacctc
     1621 ttcccagtcc acttttttgg cgtagaccac cagcaacaca atcaccgcga taaaaaacag
     1681 ccaggtgagg atcttctttg ctaagcgcca gcgcgggtgt gatttactca tcaggggttt
     1741 acccccgtgt tttcagtttc tacccgatcc tgcgtttcca ttgtcggttg tgcgggggga
     1801 tcaacctgcg tcagacgtgg tgtgtgtgcc ggaagccagc caaccagcgc cgggaagtgg
     1861 cgtaaaaagt ggaacgccag cacgcttttg gtcaggttcc accaggtgcg tttgggcagc
     1921 atggtttcat ccacctgctg acaatctgcg gcaataatgc cgttcagatt atcgcgcagc
     1981 gtctggttaa aatgacgatc gtggatgatg acatttgctt cgagattcag tgacaaactg
     2041 agcggatcga gattactgga ccctactgtc gcccagtgat cgtccatcaa tgccactttg
     2101 ccgtggagcg ggcggcggcg gtactcaaaa acctgaacgc cgcctttaac cagatagtta
     2161 tacagcaagc gcgcaccgac tctgacaatc ggcatatccg gttcgccctg aatgatcagt
     2221 ttgatccgca ccccgcgccg tgccgcttta cgcaaggcgt gtaaaaatcg atagccgggg
     2281 aagaagtagg cgttggcgat aatcacttcc cgctgcgcct gagtgagcat tttcagataa
     2341 tggcgttcaa tatcatcgcg atgttcttcg ttatcgcgcc agaccagcaa tacctgcgct
     2401 tctcccggct ggcggttctc ttccgctttg tgatgacgtc gccaccagcg tcgtgccgcg
     2461 ctctgtctag gcaggttttc cagctcaaac tggagaatat cttcgacaat cggcccttca
     2521 aggcgtaccg cgtaatcctg tttagcctct ggaccgtagc tggacatatg ctcggcggag
     2581 taattcagcc cgccaataaa ggcgatacgc gcgtcgatca ccacaatttt gcgatgcatc
     2641 cggcgaaaca cattggtgcg cataccaaaa aggcgagggc ggggatcgta gtagcggaac
     2701 actacgccag ctgccgtcag ttcattgaca aactcatcgc tgagatccgg cgaaccgtag
     2761 ccatccagca agacttccgc tttaaccccg cgttgcgctg ctgccagtag tgccgcatgc
     2821 agctgtttgc cgacgttatc ctcaaaccag ataaacgttt caagaatgat gcgttcttgt
     2881 gcctcgccaa tcgccttaaa caccgcggga taatattgct cgccgttttc cagcaactgg
     2941 atcttattgc cttcgcgcca gctacatttc ataaatgaat ctccgcactt aaaggggcat
     3001 gatcagaaag gtgtcgccat gtccgcagcg gcaacgcggt tggcgcgctg gcgctggcat
     3061 ttttgacgta gatcctgtcc agtcgtagta gaggaaattg caccggaaac gtgcgcgccg
     3121 ggcgtccgtg ggcgcgggta aaaatctcat ccagtccggc ctgcactttt aacggatgat
     3181 tagctttttg ccgccagtca ttgaaatcac ccgccaccaa taccggttcg ccgtccggta
     3241 gctcattcac ccattcggcg agcatcgcaa gctgcgcctg acggtgcgcc tcacgcaggc
     3301 ccagatgtac gcacatcaca tgaatcgctt ttccggtcat cggcggcaca atgcggcagt
     3361 agagcacgcc gcgcttttcc gcaccatcga ccgaaacatc gcgattctca taatgttcaa
     3421 tgggataacg cgacagtacg gcgttgccgt gatgcccttc cgggtatacg gcattgcgac
     3481 cgtaggcaaa atcgctccac atagtgtcgg cgagaaactc gtagtgcgag gtatcgggcc
     3541 agttttccac atgcagcgga tgaacttcgt gcgcgcccat cacttcctgc aggcaaacaa
     3601 tatcggcgct gacggtacgc acggcatcgc gaagttccgg caaaatgaag cgtcggttaa
     3661 acgcggtaaa gcctttgtga atgttgatgg tgagcacctt gaacgaaaat tgttgtgttt
     3721 gatcgggcat aattttcctg tctttgcctc ttatctcatt gaaatagtgt agtcggcgtc
     3781 acaaaaaggt gcggtcttac ggaattttcc gtaaagttcg gtactctgag taagtagaga
     3841 taaattcttc aggagagaag ccatgaagtg gcaacaacgt gttcgtgtcg caacgggtct
     3901 aagttgctgg cagattatgt tgcatttact ggtagtggcg ctgctggtgg tgggctggat
     3961 gagtaagact ctggttcacg tcggcgtggg attatgcgca ctgtattgtg tcacggtagt
     4021 gatgatgctg gtgtttcagc gccaccccga gcaacgctgg cgtgaggtgg cagacgtgct
     4081 ggaagagctg accacgacct ggtattttgg cgcagcgctg attgtgctgt ggctgttgtc
     4141 ccgcgtactg gaaaacaact ttttgctggc aattgcaggg ctggcgatcc ttgctggccc
     4201 ggcggtagtg tctttgctgg cgaaagataa gaagttacat caccttacgt ctaaacatcg
     4261 cgtacgccgc tgaccctgtc gtggccgtta tcaccagtag cggccacaaa ctattccaca
     4321 caatatccag actcgcatcc ttcaaataaa tctgcttggt aatgtccgta aagtggcgaa
     4381 tagggttaat ccacgtcaga ttttgcagcc acactggcat gttttccacc ggcgaaacgt
     4441 aaccggaaag gagaatggcg ggcatcataa aaacaaacac gccgataaac gcctgctgtt
     4501 gtgtcgagca gagtgatgaa atcaacagac cgaatcccac cagcgataaa ccgtaaatca
     4561 ccatcgtaaa gtagaacagc gctagcgatc cggcgaaggg gatttgatac gcccagatac
     4621 caatcgccag cacaatggtg gcctggaagg tggcgacaat taacgccggt acggctttgc
     4681 cgatgaagat ctgccaggtg gtgagcggcg aaaccagtag ctgatcgagc gtaccttgtt
     4741 cacgttcgcg ggcgacggaa agtgaagtga cgatcattac gccgatagtg gtgatcatgg
     4801 cgatcagcga cggcaccaca aaccatttgt agtcgagatt cgggttatac cagttgcgta
     4861 ccaccagctc gctgttgttg ggtttcggtt ttccttccag cagctcctgc tgataatttt
     4921 tgacgatctg ttgcaggtag ttggcggcaa tttgcgcact gttggagtta cgcccgtcga
     4981 ggatcaactg caacggcgcg gtctggaagg tatccagttt gcgcgagaag tcagccggga
     5041 aacgcaccag tagtaacgcc ttttgtgtgt cgatggtcgg gcggatctcc tgtgggcttt
     5101 tcagcagcag cacatgtgta aaggcgctgg cgcgggcaaa acgttgagta agttccaccg
     5161 aatgctcgcc gttatcttca tcgtagatgg cgatggtggc gttagtcact tccagcgtgg
     5221 cggcgaacgg gaacaggatc acctgaatta gcacgggtaa aatcagaatc gcgcgggttt
     5281 gtggttcgcg cagcaacgac tgcaactctt tgcggattaa cgtccataag cgatgaaaca
     5341 tgctcttctc cctaatccag ccgacgtttg gttttcagcc acgtcaggcc gataaacatc
     5401 accgccgaag cgatcaaaaa cagcacgttt accaccagca ccactggaat attcccggcg
     5461 aggaacaggc tttgcagggt gctgacgaaa taacgagcgg gaataatgta cgtcaccgcg
     5521 cggatcaccg cgggcatact gtcgatctga aaaataaagc cggaaagcat aatcgacggc
     5581 agaaaagcgg cgttcagggc gacctgagcg gcattgaact ggttgcgggt aatcgtggaa
     5641 atcagcagcc ccattcccag ggtactgagt aaaaacaggc tggagataaa aaacagaatc
     5701 agcagcgaac cgcgatacgg cacgccgaga ataaacactg acaccagcat acacagcaac
     5761 atcgccagca tcccgagaaa gtaataaggg atcagcttac acagcagcag ttccgtgcgg
     5821 gtaatctccg tagagagcag agcctccatg gtgccgcgtt cccattctcg cgccaccacc
     5881 agcgaggtga gaatcgcgcc gatgaccgtc atgataatgg tcaccgcacc cgggataata
     5941 aagtgctggc ttatcgccgc cgggttaaac cagtagcggg tttgtacatc aataagcggt
     6001 tcaaaagtct gcccgttgtc ctccgctcgc tgcatttgcc agatctgcca gatcccttcg
     6061 acatacccct gcacaaagtt agcggtattc ggctcactgc cgtcggtgat cacctgaatc
     6121 ggtgcggtgg cgttggcgcg ctccatctgt tcggcgaaat ccacgggaat aaccaccaga
     6181 ccgcgaattt tccccgactg cattttggcg atcagttcct gacggttatc gctgatggtg
     6241 gcgtcgatgt agggcgaacc ggtcatggtg tgggtgaaat ccagcgccgc ttcgctacgc
     6301 tgttccagca aaatcccgac ccgcagcttg ctggagtcga ggttaatgcc gtaaccaaaa
     6361 ataaacagca gtagcagtgg gatcactacc gcaatcagcc agctactcgg atcgcgaacg
     6421 atctgccgcg tctctttgac gcacagcgcc cgcacgcgat gccaggaaag gatcgggtta
     6481 ctcattgcta tgctccttat cccagtcgtg gatcaactga ataaaggctt gctccatggt
     6541 gggatcgggt tgctcatcgt tagctgactg tgctttcaaa tcgtccggcg taccgctggc
     6601 gattaatttc ccacggtaca ccaggccgat gcggtcgcaa tattccgctt catccataaa
     6661 gtgggtggtg accatcaccg tgacgccttt ctctaccatg ctgttgatgt gcagccaaaa
     6721 ttcacggcgg gtgagggggt caacgccgga agtcggttcg tcgagaaaga gaatatccgg
     6781 ttcatgcatc agcgaacagg ccagcgccag ccgctgttta aaacctaatg gcagttcatc
     6841 ggtggcgtgt gaggcaatac ttttcaggcc aaatgcctcg ctcatacggg agattttttc
     6901 gttctgcgcc cgaccgcgta agccatacac accagagaaa aagcgtaaat tctgttcgac
     6961 cgtcaggttg ccgtagagcg aaaatttttg cgccatatag ccgagatgct ggcgcgcttt
     7021 accggaactc tctttcagat ccatccccag caccagcgcc tggccggaag tcggcaccag
     7081 caaaccgcac atcatcttaa aggtggtcga tttacccgcg ccgtttggcc ccagcaaacc
     7141 aaaaatctcc ccacgtttaa cggcaaagtt gacgtgatcg gtggcggcaa aatcaccaaa
     7201 tttcttggtc agttctttcg cttcgatcac cgtctcgccg ggtgtgcctt ctaccgtatg
     7261 caatattgca cccagcggcg attctgaggt tccggcaccg cccagcaaat caataaacgc
     7321 atcttcaaaa cgcggcgtag tttcgttgat gttgatttcc ggcatcccgt cggcatggcg
     7381 aatatcgtct ggtgtggcct cttttttgag gatcagacgt accgatttcc cctgaatcat
     7441 gccatcgctg acctgcggca gtttcaaggc gcgttgcaac agtttgcggt tgccctcgtg
     7501 tggactggtc atcagaaagc tgcgtccggc catggtttgt gtcagggctt ttggttctcc
     7561 ctgatacagc aactcgcctt cgttcatcag taacacgtca cggcactgct cggcttcgtc
     7621 gagatacgag gtactccaga ggattaacat cccttcgccc gccagctcat gcaccatctg
     7681 ccacagttcg cgccgtgaga tagggtcaac gccgacgccg ggttcatcga gcagcaacac
     7741 tttcggttcg cccaccaggg tacaggccag gccgagtttt tgtttcatcc caccggagag
     7801 cttgcccgcc aggcgtccgg taaacggccc aagagacgta aactccagca ggcgagcaaa
     7861 agtttgctta cgtgcctcgc cggtgacgct gcgcaaatcc gcgtacagat tgaggttctc
     7921 catcaccgtg agatcttcat acagaccaaa tttctgcggc atatagccga gcacggcgtg
     7981 cagcgcgccg tcgtttttga tcggatcaaa gccaatcacc gtggcactgc cgctgtcggg
     8041 tttcagtaat cccgccaaca tccgcatcag tgtggtttta cctgcaccgt ccggccccac
     8101 caaccccgtc acataaccgg cgtgaatggt acaatcgagc ggcgcgacgg cgggcttgtc
     8161 catgcccgga aagcgttttt ccaggccgtt cagcgtgata acggcatcat tcatgtcctg
     8221 cctcgtcacc gaattgtacc gtcactggca ttccctggcg taacgcatca tcggcgtcgg
     8281 tcaccacaat acgcaggcga tagacgaggt cggtacgcag atccggtgtt tcgacggttt
     8341 tcggggtaaa ttcagcagtc ggcgaaacga aaccaatctg cccgtgatac ggcttgtccg
     8401 ggcgaccatc ggtataaagc agcactttgc gccccggctg ggcctggtca agattccgtt
     8461 catcaacata agcgcgcacc cacaccggac gcgttagtga aacggtaaac accgtgccac
     8521 cttcattgag aaccgtgcct ggctctaccg cgcgcgttaa cagcgtgcca tcagacggtg
     8581 ctatcaacgt tgagtcctgt aaattcagct ccgcctgcgc cagttgcgcc tgcgcctgtt
     8641 cgaggctggc tttcgcctga gcgatgtcct gttcacggtt accggaacgg tactgacgca
     8701 atttatcctg tgctgatttc agcgttgcct gtgcctggtc gcgcgaggag cgggcatttt
     8761 ccaggtcatt tgccgaaatg gtgcggcttt tccacagtcc ttgctggcgg ttatagaagt
     8821 tctgcgcata gtcataggcg gcttgcgcct gtttcaccgc tgcggcggcc tgagcgattt
     8881 cttcatcgcg atacccggca agcatcagat catactgcgc ctgtgccacc gagacgcccg
     8941 ctttcgcctg catcagggca atctcatacg gcttgtgatc cagttcgccc agcacctggc
     9001 ccgctttgat agcatcacct tcgtccaccg ccagcgattc aacgcgcccc ccaacacgga
     9061 aactaagatt caccgtacga atatccacgt tgccatacag cgtcaggccg ttatcctggc
     9121 ggctttgata ccaccagtag cctccggcaa ccacggcggc aagtaccact accgccagtc
     9181 cgatcacgac aggttttttc atcactacaa actcctttgc gataatcctt gcaaaatcag
     9241 gtcgatatga caggtcaccg tctggttgat cagttcggtc ttttcttcat cgaacgcggt
     9301 ccagccggta cgtaacagaa ttgtttcttt gccaagacgg aacgccagaa tctcaccaat
     9361 caacgcatgg gtatgaagga tcatgcgggt gtcattagcg tcgcagccgg tccaggcggc
     9421 aatcaggcgt gtcaggtggc tgtgtagcgg actaatcacc tgttcgtgca ccaggtggta
     9481 ggctgccgtg ggagagagct gctcacggga gataaacttg ctgaggttga cggtgtcatc
     9541 ctgggtgagc agcttaatca tgttcctgca ggcgcgaagg atcagttcac ggatggcagc
     9601 ccgatcaggc tgtggttggg cgaacaagcg ttcggcttcc tcggcatgcg gacggaactg
     9661 ctcgccaata aaatcggcaa tccactgggc gcaggcgagg tacaaatctt ctttcgaacc
     9721 gaagtagtag gtgatggcag cgatattctg cccggcctgg gcggctatct cgcgggtggt
     9781 ggcgttcatt ccatattcac caaactgcgc cagtgcggca gcaatcagct gttttttcgc
     9841 ctgttcaccc ttgattgtca tggcaggatt attcatcgca cagtccattc ttaatcaaat
     9901 gattgattaa gattatgact ccatatggga gttgtccagt atggctaaga attttagcta
     9961 cgccagtcac agggataatt tatgcgctgc gtcacaaaaa ctgctacact ccgctccctc
    10021 atgacattgt ggtttttgtc attttccttt tcagtatctc cctgaaaact acaccggtaa
    10081 cggtcggggc ggttcggagt agttatgtct ttcgattctt tgggtttaag ccctgatatc
    10141 ctgcgcgccg ttgccgagca gggttaccgt gaacccaccc ctattcagca gcaggcgatc
    10201 cctgctgtgc tggaaggccg cgacctgatg gctagcgccc agaccggcac cggcaaaaca
    10261 gcgggcttta cgctgccgct gttgcaacac ctgatcactc gccagccgca cgccaaaggg
    10321 cgtcgtccgg tacgtgcgct cattcttacc ccgacccgtg aactggcggc acagattggc
    10381 gaaaacgtcc gtgattacag caaatacctg aacattcgtt cgctggtggt gtttggcggt
    10441 gtcagcatta acccacaaat gatgaagttg cgcggcggcg ttgatgtgct ggtggcaacg
    10501 ccgggacgtt tgctggacct ggaacatcaa aatgcagtga agctggatca ggttgaaatc
    10561 ctcgtcctcg atgaagctga ccgcatgctc gacatgggct ttatccacga tatccgtcgc
    10621 gtgttaacaa aactgccagc gaaacgccag aacctgctgt tctctgcgac cttctctgac
    10681 gatattaaag ccctggcgga aaaactgttg cacaacccgc tggaaatcga agtggcacgc
    10741 cgcaataccg cgtctgatca ggtgacccag cacgttcact ttgtcgataa gaaacgcaaa
    10801 cgcgaattgc tgtcgcacat gattggtaaa gggaactggc agcaggtgct ggtgtttacc
    10861 cgtaccaaac acggcgctaa ccatctggct gagcagctca ataaagatgg catccgtagt
    10921 gtggcgatcc acgggaataa atcgcaaggt gcgcgtaccc gtgcgctggc tgattttaaa
    10981 tcgggtgaca ttcgcgtact ggtggcgacc gatatcgctg cgcgcggtct ggatatcgaa
    11041 gagttgccgc acgtcgtcaa ctatgaactg ccaaacgtac cggaagatta tgtccaccgt
    11101 atcgggcgta ccggtcgtgc ggctgctacc ggtgaagcgt tgtcgctggt gtgtgttgat
    11161 gaacacaaac tgctgcgtga tatcgaaaaa ctgctgaaaa aagagatccc gcgcattgcg
    11221 attccgggct atgagccgga cccgtcaatc aaagccgaac cgatccagaa cggtcgccag
    11281 caacgtggcg gcatcaggct gatgaacaaa cgcaaaactg cctgatgcgc tacgcttatc
    11341 aggcctacgt gaactctgca atatattgaa tttgcatgct tttgtaggcc ggataaggcg
    11401 tttacgccgc atccggcatt tcacaacaag cacttgtcag caatttgaga acacgggaaa
    11461 aaatttatct gttttaccgc ccataagcca ccgagacgtt acttctctat agccaattgt
    11521 tcgcgcaact ccattaaaag gtagcccagt cgattcttgc ccttaccatt accaccgtct
    11581 ccccagtaag catcgttttc cgtatgctca accagttttg cgggcgcggt tgccagcaag
    11641 agcacacgca gttctgcatg ctgttcgaat ttagcgcgaa gtgctttacg catcacctgt
    11701 tctttaaccg actcccagtt tttacgaagt ggcttggaac gattacgccc catgcgagcg
    11761 gcgaccatgg gggaagaaac ccgacggatc tcttcgcggt atttttcatc aaggaatttt
    11821 tgtgcctgaa aatagtgttc ggaggtgggc caggtattcc cgtcaacctt gatgggcctg
    11881 gcggcaaagt tagaaaaatc accgtagtca tcgctggtgc tgtagaaatt tatgatggtg
    11941 tcttgcatga cgtgttggat cctttgtgct cgaacgggca ttaaaccgca ttatgttggt
    12001 ggttattgcg agccgctttc cagaaacaga aaaaccatta cccctgaaaa ccgaaaaatg
    12061 ccacaatatt ggctgtttat acagtatttc aggttttctc atggcattaa ccgccgcgct
    12121 taaagcgcaa attgccgcct ggtataaggc gcttcaggaa cagatccccg actttattcc
    12181 ccgtgcgccg cagcggcaga tgattgcgga cgtcgccaaa acgctggccg gagaagaagg
    12241 gcggcatctg gcgattgaag cccccaccgg cgttgggaaa acgctctcct atttgatccc
    12301 cggcatcgcc attgcccgcg aagagcaaaa aacgctggtg gtgagtaccg ccaacgtggc
    12361 gttgcaggat cagatttaca gcaaagattt accgctgctg aaaaagatca ttcccgatct
    12421 taaattcact gccgctttcg ggcgtgggcg ctacgtttgt ccacgtaatc tgacggcgct
    12481 cgccagcact gaacccacgc aacaggatct gctggcgttt cttgacgacg aactgacgcc
    12541 aaataatcag gaagagcaaa aacgttgtgc gaagctgaag ggcgatctcg acacttataa
    12601 atgggatggt ctgcgcgatc atacggatat cgctatagat gatgatctct ggcgtcgttt
    12661 gagtaccgac aaagccagct gcctcaaccg taactgttac tactatcgtg aatgcccgtt
    12721 ttttgttact cgtcgggaga ttcaggaagc ggaagtggtg gtggcaaacc atgcgctggt
    12781 gatggcggcg atggaaagcg aagccgtact gcctgacccg aaaaatctgc tgctggtgct
    12841 ggacgaaggt catcacctgc cagatgtggc ccgggatgcg ctggagatga gcgctgaaat
    12901 caccgtgccc tggtatcgac tacagctgga cttgttcacg aaactggtcg ctacctgcat
    12961 ggagcagttt cgcccgaaga ccatcccacc gctggcgatc cctgaacgtt tgaatgcgca
    13021 ttgtgaagag ttgtatgagc ttatcgcctc gttaaacaac attctcaatc tctacatgcc
    13081 tgccgggcag gaggcagagc accgttttgc gatgggcgaa ctgcctgatg aattgctgga
    13141 gatctgccag cggctggcaa aactcaccga gatgctgcgt ggtctggcgg agttatttct
    13201 taacgattta agtgagaaaa ccggcagcca cgatattgtg cgtctgcatc ggttgatttt
    13261 acagatgaac cgcgcgctgg ggatgtttga ggtgcaaagc aaactctggc ggctggcgtc
    13321 tctggcgcaa tcttccggtg cgccggtgac caaatgggcg acgcgggaag agcgcgaagg
    13381 gcagctacac ctctggtttc actgcgtggg aatacgtgtc agcgatcagc tggaaaggct
    13441 gctgtggcgc agtattccgc acattattgt cacctccgca accttgcgtt cgctgaatag
    13501 tttttcgcgt ttgcaggaga tgagtggtct gaaagagaaa gcgggcgacc gttttgtggc
    13561 gctggattcc ccctttaacc actgcgaaca gggcaaaatt gttattcccc ggatgcgctt
    13621 tgagccttcc atcgacaacg aagagcaaca cattgccgaa atggcggcct ttttccgtga
    13681 acaggtggag agcaaaaaat atctcggtat gttggtactg tttgccagcg gacgggcgat
    13741 gcagcgcttt ctcgactatg tgacggattt acgcctgatg ttgctggtgc agggcgatca
    13801 gccgcgttat cgcttagttg aactgcaccg caaacgcgtt gccaacggtg agcgtagcgt
    13861 gctggtgggc ttacagtcat ttgccgaagg gcttgatttg aaaggtgatc tgctcagcca
    13921 ggtgcatatc cacaaaatcg cttttccgcc catcgacagc ccggtggtga tcaccgaagg
    13981 ggaatggctg aaaagcctca accgctatcc gtttgaggtg caaagcctgc cgagcgcctc
    14041 gtttaacctg attcagcagg ttgggcgact gattcgaagc cacggttgct ggggcgaagt
    14101 ggttatctac gacaaacgct tgctgacaaa aaattatggc aagcgactac tggatgcatt
    14161 accggtattt ccgatagagc aaccggaagt ccctgaaggt atagttaaaa agaaagaaaa
    14221 aacgaaatcc ccacgccgtc ggcggcgtta atgatgtgag tcaggtaagg agtcgtaaat
    14281 ggactatcgc aaaatcatta aagagatcgg gcgcgggaaa aaccacgcgc gcgatttaga
    14341 ccgggatact gcgcgcggtc tgtatgctca tatgctcaat ggtgaagtcc ctgaccttga
    14401 gttgggcggc gtattgattg cgctgcgtat caaaggggaa ggggaagcag agatgctcgg
    14461 cttttacgaa gccatgcaaa atcacaccat caagctgacg ccgcctgcag gcaagccgat
    14521 gccgattgtc atccccagtt acaacggcgc gcgtaaacag gctaacctga cgccattatt
    14581 ggcgattctg ctgcataaac tcggttttcc tgtggtggtt cacggagtta gcgaagatcc
    14641 aactcgcgtg ctgacagaaa ccattttcga actgatgggt attacaccaa cgttgcacgg
    14701 cgggcaggcg caggcgaagc tcgacgaaca tcaaccggtg tttatgcctg tcggcgcgtt
    14761 ttgcccgccg ctggaaaaac aactggcgat gcgctggcgg atgggcgtgc gtaacagtgc
    14821 acataccctg gcgaaactgg cgacgccatt tgccgaaggt gaggcactgt gtctttccag
    14881 cgtttcgcat ccggaataca ttggacgcgt cgcgaagttc tttagcgata tcggcgggtg
    14941 ggcgttacta atgcatggta ctgaaggcga agtgtatgct aatccgcagc gctgcccgca
    15001 gatcaatctc attgaccgtg aagggatgcg ggtgctgtat gaaaaacagg accctgctgg
    15061 tagcgagtta ctgccacaag caaaagatcc ggaaaccacg gcgcagtgga ctgagcgttg
    15121 cctggctggc agcgaagcga ttcccgaatc gctgaaaatc cagatggctt gctgcctggt
    15181 ggctacgggt gaagcggcaa ctatcagcga tggcctggcg cgcgttaatc aggcatttta
    15241 atttttatct cagccggatg agccacgtca tccggctttt accccaccgc aatatgaaat
    15301 tcctgcatct ttattgacct tcccgcgccc ggcgtgcagc ataaaaaaac aacaaacaca
    15361 taacataaac aggagttaac catggaaagt ggtcatcgct ttgatgctca gacgctgcac
    15421 agttttattc aggctgtatt tcgtcagatg ggtagcgagg aacaagaagc gaaattagtt
    15481 gccgatcatt taatcgcggc aaacctggca gggcatgatt ctcatggtat tggcatgatc
    15541 ccaagctatg tgcgttcctg gagtcagggg catctgcaaa ttaaccatca tgccaaaatc
    15601 gttaaagagg cgggggcggc agtcacgctc gatggcgatc gcgcattcgg tcaggtcgcg
    15661 gcacacgaag cgatggcgct ggggattgag aaagcgcatc agcacggtat tgctgccgtg
    15721 gcgctacata actcgcatca tatcggccgt atcggttact gggcggagca gtgttcagcg
    15781 gcggggtttg tctctatcca ctttgttagc gtggtcggta ttccaatggt cgcgccgttc
    15841 cacggtcgcg acagccgctt tggcaccaat cccttctgtg tggttttccc tcgtaaagat
    15901 aatttcccgc tgttgcttga ttacgccacc agcgccattg catttggcaa aacccgcgtc
    15961 gcctggcata aaggcgtccc cgtgccgcca ggttgcctga ttgacgttaa cggcgtgccg
    16021 acgaccaatc cggcggtaat gcaggagtcg ccgttggggt cgctgttgac ctttgccgaa
    16081 cataaaggct acgcccttgc agcgatgtgt gaaattcttg gcggggcgct ttctggcggt
    16141 aaaacgacgc atcaggaaac gttgcaaacc agccccgatg ccattcttaa ctgcatgacc
    16201 actatcatca tcaacccgga actgttcggc gcgccggatt gtaacgcgca gaccgaagcc
    16261 tttgccgagt gggtgaaagc ctcgccgcat gatgacgata agccgatttt gctaccgggc
    16321 gagtgggaag tgaacacgcg tcgcgaacgg caggagcagg ggattccatt ggatgcggga
    16381 agctggcagg ccatttgtga tgcggcgcgg cagattggta tgtcggaaga gacgttgcag
    16441 gctttctgtc agcagttagc cagctaaaaa aaagcccgtc cagtggcgga cgggcaaaca
    16501 agggtaacat aggatcaatg agggttagag catatgcgtc tgtcggcaaa cagacaggga
    16561 aatacttgtg ctggacgtag cgtaaacgcc tgatccgtcc aacggttcgg attttgtagg
    16621 cctgataaga cgcggtagcg tcgcatcagg catctgttgc cggatgcggc ataaacgcct
    16681 tatccgtcct acgaattact tataaattac cgcagtaccg cttaatttat tgttgttggt
    16741 ggcggaagtg atgctgtatc cactggcacc ggctgccgcc gctttctcag ccagtttcgc
    16801 ttccagggca tcgagggtgg atgcgccatc ggcagaaacc acgccgattt tattcatgtt
    16861 ctgtgcctgg gatgccgtta ccggttccgc cgcgaacacg ccaaatgaca gagttgaaag
    16921 agccatagca gcaacaacag tattgatagt tttcataatt aatctctcgc aggtgatttt
    16981 tgtataagtg acgttgtttc gtcgatgtga tgagtatcac gtttttttac gagagataaa
    17041 atcgaagaga attgaccgcc ttgttcaaat aaattgattg atatctaaca tactgataaa
    17101 ccatttattt gttgtactta tggacttgtt ttatgccgcg tggcagtcac agcagcgtta
    17161 aaggcacgga atgccacgca aaatacaaat tatactcgct atgcatctcg ccagttgtag
    17221 ttaacgtaac tgtctgtctt tcgaacctct gcgattatat cctgtatacg ctggtttttg
    17281 taaatctttc tcctgctgac aatgaataca taagcgcacg ctaggaatgg cttcccgacg
    17341 ggcttgcggg atgggggcac cgcactcttc acattcatac aggctttcgc cacgcggaat
    17401 ttcaccccga gcgcgggcaa tcgcatcttc aattgtactg ttgatctgtt cgttgacggc
    17461 gtcatcgtta gcccaagcgg atgccatatc gtcctcccca tatcaatgct tgtacagtta
    17521 agtgtagcta atatagggac gaacctgggc agttcaagcg tcagatctcc gaccattccc
    17581 gcagcagatt atgataaaga ttgagcagcg acaggatctc ttcattttca ccatagcggc
    17641 ttttcagcga ctgaatattt ttgtccagtt caaacagcat ggcgcgcttt ttatcatcgc
    17701 ggatcatcga ctggatccac ataaatgatg ccactcgtac gccgcgggtt acgggtgtca
    17761 cgcaatgcag gctgctggag ggatacaaca ccagatcacc tgccgggagt tttacccgat
    17821 gttgtccaaa ggtgtcatta acgaccagtt cgccgccgtc gtagctttgt ggatcgctta
    17881 aaaacagcgt ggcagaaagg tcagtgcgca tccagccgtt ttgtggatgg ctgcgtactg
    17941 cgccatccac atggaaacca taggtttcat tgttctgata gcgattaaac agcggcgtgg
    18001 aaagggtacg cggcaaggca gcggcaaaga acaatgcatg ttggttaacc gcgttcagca
    18061 cctcattttg cagtgcggcg tataacgcgc tgcgggtgtc tacttgctga ttgttcttaa
    18121 cttgtgcacc ttgtgctccg gtggtgacgc gtccatccac ccattcggct tgttccagtt
    18181 gttcgcgaaa atgggcgacg tcctgtggcg ataacacgcc gggaatgtgg tacatcatac
    18241 aagttctcca aaaagtgggg cctgcgcccc acatctgaat cagaaatgca tattggctgt
    18301 gagcaagaag gttcttggct cgcccgggtg ataacggtag ccgctcttgt tgattgaggc
    18361 gacgtaatcg gtatcaaaca ggttgtaaac gtttagctgg aagtcgagat tgcgattaac
    18421 ccgatacccc agtttggcat cggcgaccca gtaaccttct gtaaatgctg gcgttcccac
    18481 cgcgccgtct gaacctttat gtatactgcc gatatagcgt gcgcccgcgc caacagagat
    18541 atcatcggtt gcctgatatt ggctccataa ggtgaaggcg tgctccgggg tatacggcag
    18601 cgatgaggaa ccatcctggg caacatcttt gccgtttttg atggttgctt tttgctgggt
    18661 atagccgcca atcacctgcc acgcgggagt gatattccct gccacggata tctcatagcc
    18721 ttcgacgcgt ttcttaccgt attgcgagta agtcccgtca tcattttgct caacttcatt
    18781 ttcgatatca gtacggaaca gcgcggcggt gagcaacagg cgtttatcca gaacctgcca
    18841 tttggtgcca atctcgctgg tgttggcttt ttgcggttta aaatcggtgc ggttggcact
    18901 gttaccactg ccagactgcg caagggcgaa gttgttgccg cccggaggct gctgggaaac
    18961 ggcatagtta atatagacat tgccgttttc cgtcaggtgg tacagcgccc cggctttcca
    19021 gttcaccaga ttgcccgact tggcggtgtc gacggtggtg actggagaac cttttgccac
    19081 accagctggg caggtgatgg caccgcgtcc gctgccgccg caggcggtgg cactgtcata
    19141 ttcagtatga taattatcca gacggatccc gccgttcagc tcaaaatcac gggtgatttg
    19201 cagcgtatcg aaggcgtaaa ttgcgaaggt atccgtctga ccattggcgt ttgcgccgtt
    19261 gcgcgtcagg ccgccgggat gaattctgct gtcaggatga taaatattta ccgccggtaa
    19321 cgtcaccgga ttaacgccat agttagtctg cgtttcacgg gtaaattcca cgccggtact
    19381 gacatcatga ccgatagaac cggtatagaa cgtcgaggtc aggttggtct ggttggtgag
    19441 aattttatta ctcacatctt tggtattcgc cgtgcgtgac taggtccagc tattcacatc
    19501 gctggtgggc tgagtaatat tcgacgcccc gcccataatc gccgtcatca ggtaatcctg
    19561 ctttacgcgc gaccaacggg tagtattgcg aatggtggtg ttatcgttga tgtcgtgctc
    19621 aaaacgcatg gtggcggtgt cggtggtcga atcgtcgtaa tcggaatccg tgccgtaaaa
    19681 gttatgagta tcaacttttc cggaatgatt cagggccgcc gctcccgcag atggggcaga
    19741 atagcccggc aaaccgatgg tcggaatgcc gccgtctggc gtgttgtgct gagtgacatg
    19801 cagataatta agatacaaac gatttgctgt accaaggcca aaagcgacag aaggggcgac
    19861 gccgtaacgc tcatttttga ctttgtcgcg tccggcatcg tgggtttttt cgcccatcac
    19921 attcaggcgc accgcggtag tatcaccaat gacctgattg acgtccagcg tgccgcggcg
    19981 gaaccaggcg ctgccaatac tggcggaggc gtcaatgccg gaatcattgc gcggctgctt
    20041 gctgatcata ttgatcgagc ctgtcggtgc gctgcgcccg tagtcggtgc cggacggccc
    20101 tttaatcact tcgacctgct cggtattgaa ggtgtcgcgc gagacgctgc cgatatcgcg
    20161 aataccatca atataaatac tgttagaggt atcggcacca cgcatataaa tggcgtcgcc
    20221 agtggtggag ttaccgttct cacccgcaaa aaacgcaccc acgc
//
LOCUS       DAZXUK010000044        20236 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZXUK010000044 DAZXUK010000000
VERSION     DAZXUK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20236
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.044"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(96..3542)
                     /gene="mfd"
                     /locus_tag="ACH3L8_001329"
     CDS             complement(96..3542)
                     /gene="mfd"
                     /locus_tag="ACH3L8_001329"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFT4084508.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(3670..4743)
                     /locus_tag="ACH3L8_001330"
     CDS             complement(3670..4743)
                     /locus_tag="ACH3L8_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFT4084509.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            5005..6204
                     /gene="lolC"
                     /locus_tag="ACH3L8_001331"
     CDS             5005..6204
                     /gene="lolC"
                     /locus_tag="ACH3L8_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFT4084510.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            6197..6898
                     /gene="lolD"
                     /locus_tag="ACH3L8_001332"
     CDS             6197..6898
                     /gene="lolD"
                     /locus_tag="ACH3L8_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFT4084511.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            6898..8142
                     /gene="lolE"
                     /locus_tag="ACH3L8_001333"
     CDS             6898..8142
                     /gene="lolE"
                     /locus_tag="ACH3L8_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFT4084512.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            8171..9082
                     /gene="nagK"
                     /locus_tag="ACH3L8_001334"
     CDS             8171..9082
                     /gene="nagK"
                     /locus_tag="ACH3L8_001334"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFT4084513.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            9098..9919
                     /gene="cobB"
                     /locus_tag="ACH3L8_001335"
     CDS             9098..9919
                     /gene="cobB"
                     /locus_tag="ACH3L8_001335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFT4084514.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(10075..11121)
                     /gene="potD"
                     /locus_tag="ACH3L8_001336"
     CDS             complement(10075..11121)
                     /gene="potD"
                     /locus_tag="ACH3L8_001336"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFT4084515.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(11118..11912)
                     /gene="potC"
                     /locus_tag="ACH3L8_001337"
     CDS             complement(11118..11912)
                     /gene="potC"
                     /locus_tag="ACH3L8_001337"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFT4084516.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(11909..12766)
                     /gene="potB"
                     /locus_tag="ACH3L8_001338"
     CDS             complement(11909..12766)
                     /gene="potB"
                     /locus_tag="ACH3L8_001338"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFT4084517.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(12750..13886)
                     /gene="potA"
                     /locus_tag="ACH3L8_001339"
     CDS             complement(12750..13886)
                     /gene="potA"
                     /locus_tag="ACH3L8_001339"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFT4084518.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            14136..15362
                     /gene="pepT"
                     /locus_tag="ACH3L8_001340"
     CDS             14136..15362
                     /gene="pepT"
                     /locus_tag="ACH3L8_001340"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFT4084519.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(15411..16532)
                     /gene="roxA"
                     /locus_tag="ACH3L8_001341"
                     /gene_synonym="ycfD"
     CDS             complement(15411..16532)
                     /gene="roxA"
                     /locus_tag="ACH3L8_001341"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFT4084520.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            16781..18010
                     /locus_tag="ACH3L8_001342"
     CDS             16781..18010
                     /locus_tag="ACH3L8_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4084521.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            18385..18573
                     /locus_tag="ACH3L8_001343"
     CDS             18385..18573
                     /locus_tag="ACH3L8_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFT4084522.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(18623..18952)
                     /locus_tag="ACH3L8_001344"
     CDS             complement(18623..18952)
                     /locus_tag="ACH3L8_001344"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084523.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(19077..19256)
                     /locus_tag="ACH3L8_001345"
     CDS             complement(19077..19256)
                     /locus_tag="ACH3L8_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084524.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            19386..19586
                     /locus_tag="ACH3L8_001346"
     CDS             19386..19586
                     /locus_tag="ACH3L8_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFT4084525.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            19576..19797
                     /locus_tag="ACH3L8_001347"
     CDS             19576..19797
                     /locus_tag="ACH3L8_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084526.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            19790..20010
                     /locus_tag="ACH3L8_001348"
                     /pseudo
     CDS             19790..20010
                     /locus_tag="ACH3L8_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20012..>20236
                     /locus_tag="ACH3L8_001349"
     CDS             20012..>20236
                     /locus_tag="ACH3L8_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084527.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHT"
BASE COUNT         5015 a         5037 c         5280 g         4904 t
ORIGIN      
        1 tgaattatgg atggtgacag tgtcggatag tgcaggaaag tgcaaagaat ttgtaaatgt
       61 tgcagatggg ggcgcagaaa cgcccccgat ttacattaag cgatcgcgtt ctcttccagt
      121 tcacgcataa actggcgtac ccattcgata cgcgttttcc gctcactcaa atcctgaata
      181 aatttcaggc gcgtcggacc atcaaggcgg taatgctgcg gctgtttttg cagcaaacca
      241 atcaaccagg ccggattaac gtgattcttc tcggcaaatt cgatcacccc gcctttctca
      301 ttaccttcca gcttcctgat ccccagtttc tgcgcttgct ggcgcagacg ggcaatatcc
      361 agcagggtac gcgccggatc tggcagcagg ccgaagcgat cgataagctc gactttgatc
      421 tcttccagtt cgttttccgt tttggcgctg gcaatacgtt tgtagaacga cagacgcgtg
      481 ttcacgtcag ggatgaaatc atctggcaat agcgacggca tccgcagctc gacttctgtt
      541 tgctggctgg tgagatcttc cagcgacggc tcgcgtccgg ctttcagtgc atcgacggcg
      601 ttttccagca actccatata cagcgagaaa ccgatggttt ccattgagcc gctttgttct
      661 tcgccaagca gttcacccgc gccgcgaatc tccagatcgt gcgttgccag cgcaaaacct
      721 gccccgagat cttccagcga ggcaatcgct tcaagacgtt tttgtgcatc ggtagtcatc
      781 gcttttggat gcggcgtcag cagccatgca tatgcctgat gatgtgaacg tccgacgcga
      841 ccgcgtaact ggtgcagctg cgccagaccg aagtgatccg cacgttcaat gataatggtg
      901 ttggctgtcg ggatgtcgat cccggtttcg ataatggtgg tacaaaccag cacgttgaaa
      961 cgttgatgat ggaaatcatt catcacccgt tccagttcgc gctcgcgcat ctgcccgtga
     1021 ccgatggcaa tccgtgcttc aggcaccagt tctgccagcc gttcggcggc tttctgaatg
     1081 ttttccacat cattgtagag ataataaacc tgtcctccgc gcaaaatttc acgcaggatc
     1141 gcctcccgga ccaccaggct gtcatactca cggacaaagg ttttaactgc cagacgacgg
     1201 gcgggcggcg tggcgataat cgacaggtca cgcattccgc tcattgccat attcagcgta
     1261 cgtgggatcg gcgttgcagt aagcgtcaga atatccacgt tcgcgcgcat cgctttaatg
     1321 cgctctttat gacgcacccc gaagcggtgt tcttcatcga caatcagcag gcctaaatct
     1381 ttaaacttga cgtcactttg cagcagtttg tgcgtaccga tcagaatatc gattttccct
     1441 tccgccactt ccgcaaggat ttgcgtctgc tctttggcgc tgcggaaacg ggagatcatt
     1501 tcgatacgta ccggccagtt ggcgaaacgg tcgcggaagt tgtcgtaatg ctgctgcgca
     1561 agaagggtgg taggcaccaa caccgctacc tgcttgtggt tatctactgc caggaaagcg
     1621 gcgcgcatcg ccacttctgt tttaccaaag ccaacatcgc cgcacaccag acgatccatt
     1681 gccagcggct gacacatgtc gctaagtacc gcattaatgg cctgcgcctg atccggcgtg
     1741 gtttcaaacg ggaagctgtc gcagaataac tgatactgct cacgatcgtg tttaaacgcg
     1801 aagccctctt tggcggcgcg ttgcgcgtag atatccagca attccgccgc cacatcacgc
     1861 actttttccg ccgctttctg ccgcgcgcgt gaccacgcat cgccgccaag tttatgcagc
     1921 ggggcgtttt cttccgcgcc acctgcgtaa cggctaatca gatgcagtga cgacaccgga
     1981 acatacagtt tggcgtcgtt ggcataggtg agcatcaggt actcgccagt aatgccgcca
     2041 gcttccagcg tggtcattcc ggcatagcga ccgacgccgt gctccagatg gaccaccggc
     2101 tgaccaatat gcagctccgc aaggttacgg atcagtgtat cggggttgat ggtgcggcga
     2161 gaatcctgac gacggcgggc aacacgttca ccgagcagat cgctttcgca gatcagcgcc
     2221 agattacgcg ccttatcgac aaaaccatgt tcggcagcgc caatcatcag ataacgccca
     2281 cggtcgctgg cttcatcaag acgcataatg cgttgcggag caattttaat tcgtgcgagc
     2341 agttcaccca gcgcttcacg gcgaccttca ctttctaccg agaacaccac cggaccgtcg
     2401 aaagtctcga ggaacttacg cagcgcatcc agcggcgctt tttgttgcgc ctgaacggcc
     2461 aggtctggca gtttctggaa acctaaattg gcattcgcgg cttttgtcgg taaatgttca
     2521 gtttttagct gcactcgggg ccagtttttc agctctgaga agagctcgtc cacccgcagc
     2581 cagagcgatt gtggtggcaa cagcgggcgc atcggatcga cgccgcgatt ctcaaaacgc
     2641 gccagcgtgt cagcctggaa acgttcggca ctggtttcca gatcgccagt attcacgagc
     2701 aaggtattgg cagggaaata actgaacagc ggtggcagtg gttcgctgaa gaacaatggc
     2761 tgccagtact cgatcccagc aggtaatgtg cctttactca cttgctggta aatatgttcc
     2821 ggatcgcgct tcacttcgaa ggtatcgcgc cactggctgc ggaacagttc aattgccgct
     2881 ttatcggtcg gaaattcgtg cgcgggcagc agattgatcg cttctacttc ctccagcgtg
     2941 cgctggctgt cgacgtcaaa cacccgcagg ctgtcgattt catcatcaaa gaaatcaaga
     3001 cgataaggca gctcactccc catcgggaag agatccagca acgcgccgcg cgtggcgtat
     3061 tcgccgtgct ccatcacctg gtcaacatgg cgataaccgg cgctgtccag ttgggttcgt
     3121 aatgcatctc gtgacaggcg ctgacctttt ttcatcacca gcgcatgacc gtggagaaaa
     3181 ctgtgtgggc aaacgcgctg cataagcgta ttcaccggaa caatcagtat gccacgctgc
     3241 atcgtcggta gctggtaaag ggtggaaagg cgcgaggaga taatgtcctg atgaggcgaa
     3301 aaactgtcgt agggaagagt ttcccagtcc gccagattca tcaccatctg atcggtgaac
     3361 tggctgattt catcatgcaa acgcagagca ttttgcatat ctggtgcaat gagtaccacc
     3421 ggaccggcgt gacgttcggc aatttccgct accagcgttg cacaggctgc gccggttaac
     3481 tcgcccagca gacgctgctc acccgctttg acgggcagcg tataacgata ttgttcaggc
     3541 ataacggttg tcagattctc gttaggatat gcctcaacat atgggggcat atctctgata
     3601 agcaatgctg ttcattatcc gtaatcgaca tggtttagca aattgaatcg ccccggctgg
     3661 ggtgatggtt taacgagagt aactgacggc agcgggcagt gaaggcgggg agaacaacag
     3721 atcgctgaat gcacgctgtg aaagttttct taccagcaag cccgcaacag tacagatgaa
     3781 caaactgaca aagggataaa ccagcaggag tgtcagttcg acttgcggcg accagcgtgc
     3841 tgcgttcatt tgcgcaagca gagttaagct gaatatttca accagaatgc gatgggtggt
     3901 gtagatagca atggtgttgg agccaatcac attcagcagg ctggtggagc gcataccgaa
     3961 tcgttgctcg tactgataaa acagcttcat gatcaccaca atcgatacca gcgacaacag
     4021 cagcgagata gtaaacaacc aggcaccgac cgccagaacg gtaagcaaag aagccatcag
     4081 cagatggcgg cgcaacggca cctctttaac acaggtcatt attgttgcgc caaaccatgc
     4141 gccaaggctg taataaggca aattgcgtat cacactgttc attccccacc acggcgtggg
     4201 aacgaaatta accgccacac tcagcagtac aaacaaggcg aatagtggca gcgccagacg
     4261 gctaaaaatt ttacatacca cgaaatagac aattaacgca tacagatacc acaagctggt
     4321 gctggcggtg atcatcccgt gcaggaactc accggtggaa tcggcataag cggcattgga
     4381 ggcattgctt aaatcgcgct caggtgccag ccactggttc agcgcactta acgccagcca
     4441 ctgcaccacg ccccaaagcg ccagcaccca gaagatgttc cagatccgtt tatcgagaca
     4501 atttccccac ggtacgctgt cgatatagcg gcgaatcaaa tagccggata taaagaaaaa
     4561 aaccggcata cgaaaggggg caaggtaaag attgaaatag atccagcatt tgctcaggac
     4621 ttccgataac ggatgctgaa aagtggtcat atgcggataa aaggtaatga ccgagtgata
     4681 aatcaccacc agacaaatac ataacccttt gatctggtta atccatagct ctttttgttt
     4741 catcagaaca acacgctctt ttttaattta catgcggttg atcctgctgg ttatgacccg
     4801 aggtgtaatg ggtatctgtc tgtattggat gatttttcag attaagatca ggcggcaaga
     4861 ttgatgataa aatatggcaa tttagccgat tgatttacgg gggcttttca gattagccct
     4921 gacgatcact tacagttcag acgtttaccc atcttgcttt cgcttatata ctcgtgtctt
     4981 tgctacagca accagacgga tttcatgtac caacctgtcg ctctatttat tggcctgcgt
     5041 tacatgcgtg ggcgtgcagt ggatcgcttc ggtcgtttcg tctcctggct ttctaccatc
     5101 ggcattaccc tcggggtgat ggcgctggtc acagtattgt cagtgatgaa cggctttgag
     5161 cgcgagctgc aaaacaacat ccttggcctg atgccacagg caattctctc ttctgagcat
     5221 ggctctctta acccgcagca actcccggaa acggcagtca aactggacgg cgttaatcgc
     5281 gtcgcaccta ttactaccgg tgatgtggta ctgcaaagcg cgcgcagcgt ggcggtcggg
     5341 gtgatgctcg gtatcgaccc ggcgcaaaaa gatccactaa cgccgtatct ggtcaatgtg
     5401 aaacaaactg acctcgagcc ggggaaatat aatgtcatcc tcggcgaaca acttgcctca
     5461 cagttgggcg tcaatcgcgg tgatcaaatc cgcgtgatgg tgccatctgc cagccagttc
     5521 acgccgatgg ggcgtattcc aagtcagcgc ctgttcaatg tgattggcac tttcgccgcc
     5581 aacagtgaag tcgatggcta tgaaatgctg gtgaatattg aggatgcctc gcgtctgatg
     5641 cgttatccgg cagccaatat taccggctgg cgtttgtggc tggatgagcc gctgagagtt
     5701 gactctttaa gtcagcaaaa actgcctgaa ggcagcaaat ggcaggactg gcgtgaccgt
     5761 aaaggcgagc tgttccaggc cgtacgcatg gaaaaaaata tgatgggctt actgctgagc
     5821 ctgattgtcg ctgttgcggc gtttaacatt attacctcgc tggggctgat ggtgatggag
     5881 aagcagggcg aagtcgcgat cctgcaaacg caaggcttaa ctccgcgaca aatcatgatg
     5941 gtctttatgg tgcaaggggc tagcgccggg attatcggtg caatcctcgg agcggcgctt
     6001 ggcgcactgc ttgccagcca gttaaataat ctgatgccga taatcggcgt cctgcttgat
     6061 ggcgcggcgc tgccggtggc tatcgaacct ttacaggtca ttgttattgc gctggtggcg
     6121 atggctatcg cgctgctgtc tacgctttac ccttcatggc gcgctgccgc cacacaaccc
     6181 gctgaggctt tacgttatga ataagatcct gttgcaatgc gacaacctgt gcaaacgcta
     6241 tcaggaaggc agtgtgcaaa ccgatgtgct gctcaatgtc agtttcagcg tgggcgaagg
     6301 tgaaatgatg gcaattgtcg gtagctctgg ttccggtaaa agtaccttgc tgcacctgtt
     6361 aggcgggctg gatacgccaa cctccggcga tgtgatcttt aacggtcagc cgatgagcaa
     6421 actgtcttca gcggcgaaag cagaactgcg caaccagaag ctgggcttta tttatcagtt
     6481 tcaccacctg ctgccggatt ttactgccct ggaaaacgtg gctatgccac tgctgattgg
     6541 caagaaaaag cccgctgaaa tcaacagctg tgcactggag atgttaaaag cggtggggct
     6601 ggagcatcgt gcgaatcacc gcccatctga actttctggc ggcgaacgcc agcgtgtagc
     6661 gattgcccgt gcgctggtca ataatccgcg tctggtactg gcggatgaac ctaccggtaa
     6721 cctcgatgcg cgtaacgccg acagcatctt ccagttgctt ggggagttga atcgcttgca
     6781 gggcaccgct ttcctggtgg tcactcacga cctgcaactg gcgaaacgta tgagccgcca
     6841 actggaaatg cgtgatggac gtctgacggc ggaactgagc ctgatggggg cggagtaatg
     6901 gcgatgcctt tatcgttatt gattggcctg cgttttagtc gcggacggcg acgcggcggc
     6961 atggtgtcgc taatctctgt catttctacc attggcattg ccctcggcgt ggcggtattg
     7021 atcgtcggct taagcgcgat gaacggcttt gaacgcgaac tgaataaccg cattctggcg
     7081 gtggtgccgc atggcgaaat cgaagcggta aatcagccat ggactaactg gcaggaagca
     7141 ctggataacg tgcagaaagt gccgggtatt gccgccgctg cgccgtatat caatttcacc
     7201 ggtctggtgg aaagtggagc gaatctgcgc gcaatccagg tgaagggcgt taacccacaa
     7261 caggaacagc gtctgagcgc attaccctcg tttgttcagg gggatgcgtg gcgcaatttt
     7321 aaagcgggcg aacagcaaat tatcatcggc aaaggcgtgg cggatgcgct gaaagtgaag
     7381 cagggcgatt gggtgtcgat catgatcccc aactcgaatc ccgagcataa actgatgcag
     7441 ccaaaacgtg tgcgtttgca cattgccggt attttgcagt tgagtggtca actcgatcac
     7501 agttttgcca tgatcccgct ggcggatgcc caacaatatc ttgatatggg ttccagcgtg
     7561 tcaggtattg cccttaaaat gacggatgtt ttcaacgcca ataagctggt acgcgatgcg
     7621 ggtgaagtga ccaacagcta tgtttatatt aaaagttgga ttggtactta cggctatatg
     7681 tatcgcgata tccagatgat ccgcgccatt atgtatctgg cgatggtact ggtgattggc
     7741 gtggcctgtt tcaacatcgt ctccacctta gtgatggcgg tgaaagacaa gagtggcgat
     7801 atcgcagtat taagaacgct gggggcgaaa gatggtttaa ttcgcgccat ctttgtctgg
     7861 tatggattgc tggcagggct gttcggcagc ctgtgtgggg tggttatcgg cgtagttgtt
     7921 tcactgcaac ttaccccgat tattgagtgg attgaaaagc tgatcggtca tcagttcctc
     7981 tccagcgata tttattttat tgacttcctg ccatcggaat tgcactggct ggacgtcttc
     8041 tacgtactgg tcacagcatt gttgctgagt cttttggcaa gttggtatcc ggcgcggcgc
     8101 gccagtaata ttgaccctgc gcgagtcctt agcggccagt aaaggcagta cattaaaaca
     8161 aggagcggca atgtattacg ggtttgatat tggtggaaca aaaattgcgc ttggcgtgtt
     8221 tgatagcggt cggcagttgc agtgggaaaa gcgggtgccg acaccgcgtg acagctatga
     8281 cgcattttta gatgcagtgt gtgagctggt agctgaagct gaccaacgtt ttggctgtaa
     8341 aggttctgtc ggcatcggta ttccgggaat gccggaaaca gaagatggta agctgtatgc
     8401 cgctaatgtc cccgctgcca gcggcaaacc gctgcgtgcc gacctgagcg cacgtcttga
     8461 tcgcgatgta cgccttgata acgatgccaa ctgttttgcc ctttcagaag cctgggatga
     8521 tgaatttacg caatatccac tggtgatggg gttaattctc ggcaccggcg ttggcggcgg
     8581 gctggttttc aacggcaaac cgattactgg taaaagctac attaccggcg agtttggcca
     8641 tatgcgtctg ccggttgatg cgttaaccat gatggggttg gatttcccgt tacgccgctg
     8701 cggctgtggt cagcatggct gcattgaaaa ttatctgtct ggtcgcggtt ttgcgtggct
     8761 gtatcaacac tattatcatc aaccgttgca ggctcccgaa attattgcgc tttatgatca
     8821 aggcgatgag caggcaaggg cgcacgttga gcgttatctg gatttattag cggtttctct
     8881 gggaaatatc ctgaccattg ttgaccctga cctggtcgtc attggtggtg gcttatcgaa
     8941 tttcccggca atcacaacgc aactggcgga caggttgcct tgtcatctct tacctgtagc
     9001 tcgtgttccg cgcattgaac gcgcgcgcca cggtgatgcg ggaggaatgc gtggtgcggc
     9061 cttcctacat ctaaccgatt aaacaacaga ggttgctatg ctgtcgcgtc ggggtcatcg
     9121 gttaagtcgt tttcgtaaaa ataaacgtcg cctgcgcgag cgtttgcgtc agcgtatttt
     9181 tttcagagat aaagtggtgc cggaagcaat ggaaaaacca agagtactcg tactgacagg
     9241 ggcaggaatt tctgcggaat caggtattcg tacctttcgc gccgcagatg gcctgtggga
     9301 agaacatcgg gttgaagatg tggcaacgcc ggaaggtttc gatcgcgatc ctgaactggt
     9361 gcaagcgttt tataacgccc gtcgtcgaca gctgcagcag ccagaaattc agcctaacgc
     9421 cgcgcatctt gcgctggcta aactgcaaga cgctctcggc gatcgctttt tgctggtaac
     9481 gcagaatata gacaacctgc atgaacgcgc aggtaatacc aatgtcattc atatgcatgg
     9541 ggaattgctg aaagttcgtt gttcgcaaag tggtcaggtt ctcgactgga ccggagacgt
     9601 tacgccagaa gataaatgcc attgttgcca gttcccggca cccttgcgcc cgcacgtcgt
     9661 ttggtttggc gaaatgccac tcggcatgga tgaaatttat atggcgttgt cgatggcgga
     9721 tgtctttatt gccattggta cttccgggca tgtctatccg gcggctgggt ttgtacacga
     9781 agccaaacta catggtgctc acaccgttga actgaatctt gaaccgagtc aggttggtaa
     9841 tgaatttgcc gagaaatatt acggcccggc aagccaggtg gtgccagaat ttgttgaaaa
     9901 gttgctggag ggattataaa gcaggaagca ttgcctgatg cgctacgctt atcaggccta
     9961 caagcggacc acaataccga cgaaagagcg ggataaaact ttactgtcct gactcattag
    10021 ccacatcctt gctaactaaa aaacgggcgg taataccacc gcccgcttgc tgaattaacg
    10081 tcctgctttc agcttctgat aatactcttc ataaatgctg ctggctgcgc caacgtcatt
    10141 ctgccattcg ccatttttaa tggtttcagc atccgggtag agcgtcttat cgtttgccac
    10201 ttctggactt aacagcttac gcgccgcaag gtttggcgtt ggataaccga tagtttcagc
    10261 aacctgtttt gccacatccg ggcgcagcag gaagttgatc aatttcagcg cgccttcttt
    10321 gtttttggca tttgccggga tcgccaggct gtccatccag aatatgccgc cttctttcgg
    10381 ccacaccacg tcaataggcg tacccgcctg gcgtgcaacg aaagcagaac cgttccagat
    10441 catgccgagg ttaacttcgc cttccatgta cgggttagcc gggttatcgg agttaaacgc
    10501 tgcgacgttt ggcatcagtt ttttcagctc gttatatgca gcttcaatct ctttcggatc
    10561 ggtggtgtta ccggagtagc ccagcttacg cagcgccatc tggaacactt cacgggcatc
    10621 gtcggtcaac agcaggctgc ctttgtactc tggcttccac agatcggccc agctggtgac
    10681 agatttcgga tccaccgcat caccgttaac accaatcgcc gtcgcacccc agatatacgg
    10741 aatggagtag tcgttattcg ggtcaaaagg cttgttgagc atgtctggat cgagattgct
    10801 gaagttcgtt aacttcgact tgtcgatctt ctggatcatc ccctctttac gcattttatc
    10861 gacgtaatag gttgaaggaa ccaccaggtc ataggcaccg tctttgtacg ttttcagctt
    10921 ggcgtacatg gtttcgttcg actcgtaagt cgaatagata accttaatac cggtttcttt
    10981 ggtgaactgt tcaagcagtc ctggcggcac gtactcggtc cagttgtaga aatacagcgt
    11041 gttgttgtca tcggcgtgag cggcgctcat gcccagtgcc agagcacccg ccgcgagcag
    11101 gtggcgtgac cattttttca tttaacgtcc cctgtgttac ctttcgtttt atcacgagca
    11161 ataagctggc tggcaataac catcaccagc gacagcacca gtaatatggt tgccagcgcg
    11221 ttaacttccg gcgatacgcc gactttgacc atcgaataaa tttttaacgg cagaatttca
    11281 taacttggtc cggtgacgaa tgaagaaact accacatcgt ccatcgacag ggtaaagctt
    11341 aacacccagc ccgccgccac cgctggcatt gccagtggca gaatgatttt tcgcagaatg
    11401 gtaaattcgc tggcaccgag atctttcgcc gcttccagca tccgcacgtc aaaacctttc
    11461 aggcgcgaat acaccgtcac caccacaaat ggcaggcaga aggtgatatg cgagaacagc
    11521 agcgaccaga agccaagctg aatacccagc agcataaaca gcaccagcag cgaaattgcc
    11581 atcacgatat ctggtgacat cattaccaca aacagcattc cgctaacgaa cggcttaccg
    11641 cgaaagcgat aacggtacag cgcaactgcc gtcagtgaac cgataagcgt agcaaacgtc
    11701 gccgaaaaca ccgccattgt tagtgaatgc tgcgctgcct gtaacaggct gtcgttgttc
    11761 atcagcaggc tataccattt ggtggtaaaa ccctgccagt tgatgccaaa gcgcgagctg
    11821 ttaaaggagt tcacaatcaa aataatgatt gggatataca ggtacgcgta gatagcggtc
    11881 ataaaaccgc cgcgaagcag tcgaccgatc attcgagttc caccttctta ttcagcaaac
    11941 gagaagcgcg ccagtaaacc agcaacatca ggcccattac gatagtcagc gtaatgctgg
    12001 tggctgcacc aaacggccag tcacgaatat tgaggaactg taccttgatg acgttaccga
    12061 tcagcaggtt tttcgcaccg cccatcaggt cggacacata gaacaggccc atcgctggca
    12121 gcatcaccag cagacatccg gcaataatcc ccggcatcgt cagtggaata atgatgcgga
    12181 taaaagtctg taacttgctg gcaccgagat cgcgcgccgc ctcaagcaac ggcttatcca
    12241 gtttttcgat actggagtac agcggcatca ccataaacgg cagcagaatg taaaccagac
    12301 cgataatcac cgcactgggc gtgaacatga tacggattgg tgtgtcgata acgcccagcc
    12361 agagcaaaaa ctcgttgaga tagcctttgg tgctgaggaa aattttcagc ccgtagatac
    12421 gaatcaatga gttggtccag aacggaacaa tcagcagaaa cagcagcagc ggacgcacct
    12481 tgtgtggcag cttcgccaga aaccaggcaa atggatagcc cagcaccagg caggcgaggg
    12541 tggcgatcag cgccatattc agcgagtgca atagcacttc aaaatagagc ggatcgagca
    12601 gacgcgtgta gttatccagt gtaaagacca ttttgacgaa actggcgtcg tcgcgggtca
    12661 aaaagctggt gccaatgatc atcaggttgg gcagaaagac aaacaacaca agccaaccga
    12721 caatagtgac aatcactaca ttctggaact tacttgtgtt cttcatcagc cagtacgacc
    12781 tcccagcttt ctacccaatt aatggccatt ttttggtcga gagagtggtc aaagtcagga
    12841 tcgtcttcat tgaagaattc gctgaccatc accatcttgc cattttccag ttcaacaacc
    12901 gactccagcg tcatgccttt gtagttacgc tcacgaacgt aaccaatcag cccttcagcg
    12961 tggttgtcgt cgttaatctc ttcaacacgt aagtcttccg ggcgcagcag aacatgcagt
    13021 ttttgccccg gttcaacggc gaagttaacg tagatattac attcgcggcc ttcaacgttg
    13081 gcgcgtacgc gctgctcgtc gagtcgctcg atcaccgtgg cgttaaacat attaatttcg
    13141 ccaatgaacc cggcaacaaa caggtttttc ggctcttcgt agatttcacg cggcgtgccg
    13201 tcttgctcaa tgcgaccatc gcgcatcacc acaatcctgt ccgacatggt gagtgcttct
    13261 tcctggtcgt gagtcacgaa aacgaatgta atgccaagct tacgctgtaa cgctttcagc
    13321 tcgttctgca tttgcttacg cagtttgtaa tccagcgctg agagcgactc atccagcaac
    13381 aacagacgag gcttgttaac caccgctcga gcaatggcga cgcgttgctg ttgaccacca
    13441 gagagctgat gcggtttacg ttgagcgaag gtttccagct gcaccatccg cagggcttcc
    13501 atcacgcggg gcgtaatttc agcagcgggg gttttttgca tgcgcaaccc aaaggccaca
    13561 ttttcgaaca cggtcatgtg ggggaaaagt gcatagcttt ggaaaacagt gttcacatag
    13621 cggttttccg ccggaacgtg ggtgatgtcc tcgttatcca gcatgatgcg tccggaatca
    13681 acagtttcca gacctgcaat caggcgaaga acggttgttt taccgcagcc agaagggcca
    13741 agcagcgtga ggaactcgcc attgttgata gtcagatcca gctggggaat gacctcttta
    13801 ccatcaaagc atttgcgaat tcccgccaat tgcaccagcg gtgaaagcga actcggttgt
    13861 ttattcaatt ttttactctg tcccatgtaa acgcaacgga tggcttaccg atgcggggtt
    13921 tgtggttaac caccttggtg actcctaatg agggcggtaa ttctacggca aaccgcttga
    13981 atcgccaatc tttgttgtga attactggct tagctttata ttcattaagg taatgctgat
    14041 aaatattccc gcttgcaggg gtaaaagtga cctgacgcaa tatttgtctt ttcttgcttc
    14101 ttaataatgt tgtcacaaaa agtgagggtg actacatgga taaactactt gagcgatttt
    14161 tgaactacgt gtctctggat acccaatcaa aagcaggggt gagacaggtt cccagcacgg
    14221 aaggccaatg gaagttattg catctgctga aagagcagct cgaagaaatg gggcttatca
    14281 atgtgacctt aagtgagaag ggcactttga tggcgacgtt accggctaac gtccctggcg
    14341 atatcccggc gattggcttt atttctcatg tggatacctc accggattgc agcggcaaaa
    14401 atgttaatcc gcaaattgtt gaaaactatc gcggtggcga tattgcgctg ggtatcggcg
    14461 atgaagtttt atcaccggtt atgttcccgg tgctgcatca gctactgggt cagacgttga
    14521 ttaccaccga tggtaaaacc ttgttaggtg ctgatgacaa agcaggtatt gcagaaatca
    14581 tgaccgcgct ggcggtattg caacagaaaa acattccgca tggtgatatt cgcgtcgcct
    14641 ttaccccgga tgaagaagtg ggcaaagggg cgaaacattt tgatgttgat gccttcgatg
    14701 cccgctgggc ttacactgtt gacggtggtg gcgtaggcga actggagttt gaaaacttca
    14761 acgccgcatc ggtcaatata aaaattgtcg gtaacaatgt tcatccgggc acggcgaaag
    14821 gagtgatggt aaatgcgctg tcgctggcgg cacgtattca tgcggaagtt ccggcggatg
    14881 aaagcccgga aatgacagaa ggctatgaag gtttctatca cctggcgagc atgaaaggca
    14941 ccgttgaacg ggccgatatg cactacatca tccgtgattt cgaccgtaaa cagtttgaag
    15001 cgcgtaaacg taaaatgatg gagatcgcca aaaaagtggg caaagggtta catcctgatt
    15061 gctacattga attggtgatt gaagacagtt actacaatat gcgcgagaaa gtggttgagc
    15121 atccgcatat tctcgatatc gcccagcagg cgatgcgtga ctgcgatatt gaaccggaac
    15181 tgaaaccgat ccgcggcggt accgacggcg cgcagttgtc gtttatggga ttaccttgcc
    15241 cgaacctgtt cactggcggt tacaactatc atggtaagca tgagtttgtg attctggaag
    15301 gtatggaaaa agcggtgcag gtgatcgtcc gtattgccga gttaacggcg caacggaagt
    15361 aagcgaaaag ggatgcggca tgtgatgctg catccggctt aaatccaaac ttacccttcg
    15421 aagaaccaat acccgctatt gaccagcgcc gcgagcatcg cgaggaatga cggatcttcc
    15481 agcgcatcgc caaaattctc cgcagtcagc gcaatgttgc tggcgagtgc atccagtgcc
    15541 ggacggtgcg gggaatcgat cttctcacca ttggcataca cgtcgtcgcc aatgcgcaat
    15601 acgcgcagac cacccaggcg caccagcaca tcaccttgtt tcagcgcatc gtagatttca
    15661 tctggctgat aaggcggttc cggcggcgcg atatccagtt catgacgtga ctgggatata
    15721 aactcgccaa accattgctt aaagtgttcc ggctggttga tcaattcgag catcatctca
    15781 cgcagtttat ccatctcttg cggcagaaca tccgcaggat gagcacgagg tggaacatcc
    15841 ggatcgctgt agtagttgcc gcccagttca cgttgcagca cataatcggc aaatccgcta
    15901 attaattccc gcgtatttgg cgcgcgaaaa cccacggaat agttcatcgc attttccagc
    15961 gcgtagcctt catgcgggaa tcctggcgga atataaagaa tatcaccagg ctccagctct
    16021 tcatcgatga tggcttcgaa cggatcgacc tgtaacagat ccggatgtgg gcagtgctgt
    16081 ttcatttgca gcttttcgcc cactcgccag cgacgacgtc cggtaccctg aatgataaac
    16141 acgtcgtact gatcgagatg cgggccgacg ccgccgccag gtacagaaaa agaaatcatc
    16201 agatcatcaa tacgccagtc cggtagttca cggaacggtc gcatcagcgc ggcggtcggc
    16261 tcatgccagt ggtttactgc ctgtaccagt aatgaccagt tggtttcacc gagatgatcg
    16321 tagctttcga acgggccgtg gctgacctgc catttgccat cctggtgact gaccagtcga
    16381 ctgtcaactt cgctttccat cgccagaccc gccaactcgt ctggagagat cgggtcaata
    16441 aaattattaa agccgcgttt taacaccacc gggcgtttct gccagtgacg ttcaagaaaa
    16501 tcgggccagt taagagtgag ttggtattcc atgttaagca tccacaggct ggtatctgca
    16561 accgattata acggatgctt aacgtaatgc gtgaagtatg gacatatttt atcaattagt
    16621 gttttttgcc gtttcataat gtttcagatt gttcattatg tttttgatta taaacagatt
    16681 tgtgattttc cttgttccag cttgtttcac gttgtagccc tttgtgcaac gatcggtgta
    16741 gttaatggtg tagttaattg tgatgaccgg aatcaaaatt atgagcagag cacttaacaa
    16801 actgagcgat acacagctga ggaaaatcaa cggcacaccc gcccaaaaaa cagcctttct
    16861 taatgacggt ggaaacctga gcgtcaggca ttcaaccagt ggccttttaa cctggtattt
    16921 cacttacagg gccggaacgg gaaggggggc accaccggaa cgcattaagc tgggaaatta
    16981 tcctgatctg agcctgaaat cagccaggga aaaagccgcc cagtgtcgcg catggctggc
    17041 agaggggaaa aatccacgtc atgagcttaa ttacaccgta caggaagcgt taaagccggt
    17101 aacggttggc gatgcgctca cctactggct tgagtcgtac gcaaaggaaa accgcgtgga
    17161 ttatgccgcc ctgaaaaagc gccttaataa tcacgtaata cagcacattg gtgctatgcc
    17221 gctggataaa tgcgagctac ggcactggct ggcctgtttt gaccaggtgg caaagcgaac
    17281 gcctgttact gccggattct tgctacagac gtgcaaacag gcgcttaagt tctgccggag
    17341 gcggcgctat gcaatcagca acgttcttga tgatatgagt gtggcggacg ttgggaaaaa
    17401 accggatata agcgagcgtg tcttaagcac caaagaactg ggcgaattat tgcaggcact
    17461 ggacaaaaaa atattctccc cctactacat cgcgttaatc cgcctcctga ttgtgttcgg
    17521 atgccggacg gtagaactga ggttatcgga gatcagcgag tgggatttta ccgaaatgct
    17581 ctggaccgtg ccgaaagaac acagcaaaac gaaggtggca atattccggc ccataccgga
    17641 agcaatactg ccgttcgtca cgcagctggt ggagcagaac aggcacacgg gcttattgct
    17701 gggggaagtg aaacaggaaa caagcgtgtc gcagtacggc agattagcgc acaggaggct
    17761 aaatcaccct cactggtcac tacatgacat ccggcgcacc tttacaacga tgctgaacga
    17821 tttaggcgtg gatcctcacg tcgtggagca gcttacaggc caccagatgc caggaatgca
    17881 gcgagtttat aatcattccc gttatctgga tgctaaacgc aatgcgctgg atatgtggac
    17941 ggagcggtta gggatactgg cgggaacaca tgaaaacgta accacgctac cagtagccag
    18001 aagaaaataa tttttttcgt gttttttcag tatgcgcata ctggatatgc gaacagatac
    18061 aacgtgcaac aatgagcaac aatgcggaac aactacgaac aagaggtgaa aaagtgtaca
    18121 gatttataag tagctgattt ttaatgtgtt actgattttt tatacactag cgaatcactc
    18181 tttaaatagc gagaaaaaag gcatgaaaca atattttatg cctttgtatt aacatgaatt
    18241 taaaggattt tatcctgatt attcctcttt ctgtaaacca tacggcctca acatttgcca
    18301 actctgaatc atcctgacac aatcagacac atcccaacgc aatagaacac gaaataactc
    18361 tttaagaaac gaaagggggc aatagtgtta agcactgatc ggtttatacg tgaaaaagaa
    18421 tgcgaaaagc taacaggcct tagccgctca tgccgctacc gcctggaaaa ggccggacaa
    18481 ttcccatcac gtcgtaaact tggcggtcgt tccgttggct ggtctttatc cgaggttctg
    18541 gcctggaagg atagctgcaa ggcagttcat taatcacgct ggcggcacgc agccgccaca
    18601 catcaatcat ctgaacacag agctataacc atgaagattg aatatacgcc agaacgtggg
    18661 cggggattcg ttcgccctgg tgaaactgga aaaccacaaa attggggttt ttcaggcata
    18721 aaaaaagcgg ccccgaaatg gagccgcctt tctgaacaga taacccgctg cgccgtttgt
    18781 gtgtgtgatc ccaaacataa gcacggggat gatagccgct atcaggctgg tgggcaatgc
    18841 aatcagtctg gttcagtgcg ttgccataac tgcgaagttc tctcctttga tgagtattct
    18901 ttaaggtact tctcaagggc aaaagaacac ggcgcgaatc gttctgattc atgctctttc
    18961 tttctgcgtc gtctttttcg tgcaggtgat gttttttcgg ttgattcttc gttggtcatt
    19021 gtgtgcgcct gtaaagcaat gcgccgtagt acctcacacc acggcgctga tagtttttat
    19081 cctttgggtt ctatgccgcg ttgctgtaat tctttgcgga ttattcgctt tatccaggca
    19141 gcaagagaat catcgccgtc ctgttgttgt gccttttcca tcttttcccg taagtcagga
    19201 tctaaacgga actggaacgg aggattacca cgcctttcgt ttttgtgtgt tgacacgtta
    19261 attacacctc aggtaatgta tttatgtgta gtgacacatt acacacaatc aacacaaaaa
    19321 gcaaagcccc gcactgtagg agcagtaacg gggcttctaa ccaacaacgt aaactaggag
    19381 ccgttatggt tgccgtaaat catataccac accttgtaca cacacaaacg gcctttgtgt
    19441 ggcgttttct ggcactgagt gccggagaat cccaaatcat ccacgtaacc gcttggacgg
    19501 aacgcgaagc gcgtagccgt tgcccgtccg gttgtgttgc tgtattcgcc gcccgtattc
    19561 gtcaggggga aacctatgca caataaaacc acaccgaacg cagcagccgc cgcactcact
    19621 acgctgatgc acgcgcttat tgatattgaa tgcacggcag agcttgcgca gggagaagaa
    19681 cagaaagacc ggacacagtt cgccctggaa tgtatccgat acatcgcaac gcggtcactg
    19741 aatgacgcta aaaatattct tgttgccgat tgtgaaaatg gggggggtta tgcgtgatga
    19801 tcgttttaat tccctgaaac aggaattttc cggcgttcct gatgatgcgg ctgatgcgct
    19861 ttcgtcaatg ccagaactta ttagagcggc ttttttctta ctttccacga gagaatataa
    19921 atcaacgggc ttgatgtact gaatatcgcc gccgattatg ctgatttcgt gacagaggtt
    19981 attttaagaa aaacgacgga cggggattaa tatgcgtgat atttaccacg aaacaataga
    20041 ccgcgcattt cttgcacttt ctcacagtga aaacatgctg gaaatattgc gcatatggct
    20101 tgaaacactt ggcgacaatg aacgcgacaa acaaaaatca agaattgcca cggcattaat
    20161 aacgcttctt gagcctgtaa taatggaact gcaagaaata gatctattgc acgacagata
    20221 taaagaacaa cacacc
//
LOCUS       DAZXUK010000045        20165 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZXUK010000045 DAZXUK010000000
VERSION     DAZXUK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20165
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.045"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            94..1209
                     /gene="adrA"
                     /locus_tag="ACH3L8_001350"
                     /gene_synonym="dgcC"
     CDS             94..1209
                     /gene="adrA"
                     /locus_tag="ACH3L8_001350"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFT4084528.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1226..2035)
                     /gene="proC"
                     /locus_tag="ACH3L8_001351"
     CDS             complement(1226..2035)
                     /gene="proC"
                     /locus_tag="ACH3L8_001351"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFT4084529.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2155..2613
                     /locus_tag="ACH3L8_001352"
     CDS             2155..2613
                     /locus_tag="ACH3L8_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFT4084530.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RECLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2796..3320
                     /gene="aroL"
                     /locus_tag="ACH3L8_001353"
     CDS             2796..3320
                     /gene="aroL"
                     /locus_tag="ACH3L8_001353"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFT4084531.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            3370..3561
                     /gene="yaiA"
                     /locus_tag="ACH3L8_001354"
     CDS             3370..3561
                     /gene="yaiA"
                     /locus_tag="ACH3L8_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFT4084532.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3819..4496
                     /locus_tag="ACH3L8_001355"
     CDS             3819..4496
                     /locus_tag="ACH3L8_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFT4084533.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4568..4852
                     /gene="ppnP"
                     /locus_tag="ACH3L8_001356"
     CDS             4568..4852
                     /gene="ppnP"
                     /locus_tag="ACH3L8_001356"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFT4084534.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            5007..5342
                     /locus_tag="ACH3L8_001357"
                     /pseudo
     CDS             5007..5342
                     /locus_tag="ACH3L8_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            5339..5794
                     /locus_tag="ACH3L8_001358"
     CDS             5339..5794
                     /locus_tag="ACH3L8_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084535.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(5872..6783)
                     /gene="rdgC"
                     /locus_tag="ACH3L8_001359"
     CDS             complement(5872..6783)
                     /gene="rdgC"
                     /locus_tag="ACH3L8_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFT4084536.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            6908..7816
                     /gene="mak"
                     /locus_tag="ACH3L8_001360"
     CDS             6908..7816
                     /gene="mak"
                     /locus_tag="ACH3L8_001360"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFT4084537.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            complement(7959..9140)
                     /gene="araJ"
                     /locus_tag="ACH3L8_001361"
     CDS             complement(7959..9140)
                     /gene="araJ"
                     /locus_tag="ACH3L8_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFT4084538.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(9266..12409)
                     /gene="sbcC"
                     /locus_tag="ACH3L8_001362"
     CDS             complement(9266..12409)
                     /gene="sbcC"
                     /locus_tag="ACH3L8_001362"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFT4084539.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(12406..13608)
                     /gene="sbcD"
                     /locus_tag="ACH3L8_001363"
     CDS             complement(12406..13608)
                     /gene="sbcD"
                     /locus_tag="ACH3L8_001363"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFT4084540.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            13798..14487
                     /gene="phoB"
                     /locus_tag="ACH3L8_001364"
     CDS             13798..14487
                     /gene="phoB"
                     /locus_tag="ACH3L8_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFT4084541.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            14545..15840
                     /gene="phoR"
                     /locus_tag="ACH3L8_001365"
     CDS             14545..15840
                     /gene="phoR"
                     /locus_tag="ACH3L8_001365"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFT4084542.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            16247..17566
                     /gene="brnQ"
                     /locus_tag="ACH3L8_001366"
     CDS             16247..17566
                     /gene="brnQ"
                     /locus_tag="ACH3L8_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFT4084543.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            17642..19015
                     /gene="proY"
                     /locus_tag="ACH3L8_001367"
     CDS             17642..19015
                     /gene="proY"
                     /locus_tag="ACH3L8_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFT4084544.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            19171..>20165
                     /gene="malZ"
                     /locus_tag="ACH3L8_001368"
     CDS             19171..>20165
                     /gene="malZ"
                     /locus_tag="ACH3L8_001368"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFT4084545.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNM"
BASE COUNT         4581 a         5030 c         5411 g         5143 t
ORIGIN      
        1 agtgctaacg ggtgagctac gaaagtggct cacccgaaat ttcatacttc tgcctttagc
       61 cccgtctcta taatttggga aaattgtttc tgaatgttcc caaaaataat gaatgatgaa
      121 aactttttca aaaaagcggc ggcgcatggg gaggaacctc ctttaactcc tcaaaacgaa
      181 catcagcggt ccgggctgcg cttcgcccgt cgcgtcagac taccccgtgc ggttggcctg
      241 gctggcatgt tcttaccgat tgcttcaacg ctggtttcgc acccgccgcc gggctggtgg
      301 tggctggtgt tggtcggctg ggcgttcgtc tggccgcatt tagcctggca gatagcgagc
      361 agggccgtcg atccgcttag ccgggaaatt tacaacttaa aaaccgatgc agtattagcg
      421 ggaatgtggg taggcgtaat gggcgtaaac gtgctgcctt ccaccgctat gttgatgatt
      481 ctgtgtctga atttgatggg ggcaggcggc ccccgtctgt ttgtcgcggg tctggtgttg
      541 atggtggttt cctgccttgt caccctcgag cttacgggca ttaccgtgtc gttcaatagt
      601 gctccgctgg aatggtggct ctcccttccc attattgtcg tttatcctct gatgtttggc
      661 tgggtcagct accagacggc aaccaaactg gcggaacata aacgcaggtt gcaggtcatg
      721 agtactcgcg acggcatgac gggcgtgtat aaccgacgtc attgggaaac tatgttacgc
      781 aatgaatttg ataactgtcg gcggcataat cgcgatgcaa cgttactgat tatcgatatc
      841 gaccatttca agagcatcaa cgatacctgg ggtcatgatg tgggcgatga agcgattgtg
      901 gcgcttaccc gacagttaca aattaccctg cgcggtagcg atgtgattgg tcggtttggc
      961 ggcgatgagt ttgcagtaat catgtccggt acgccagctg agagcgccat taccgccatg
     1021 ttacgggtgc gtgaagggct aaacacgtta cgcttgccga atacgccaca ggtaacttta
     1081 cggattagtg tgggggttgc gccgctgaac ccacaaatga gtcactatcg tgagtggttg
     1141 aaatcggcag atttggcgct ttacaaagca aagaaagccg gacgtaaccg caccgaagtg
     1201 gcggcctgac gtccggcgaa agtcatcagg atttgctgag tttttctgat ttttccatac
     1261 acttcgtcat cgcttcgatc actgcggcac ggaagccttt ctcttccagt acgcgtaccg
     1321 cttcaatggt ggtgcctccc ggtgagcaga ccatatcttt cagtgccccc ggatgttctc
     1381 ccgtttccag caccattttt gcggaaccca ttaccgcctg agcggcaaat ttatacgcct
     1441 gggcgcgtgg catcccgccc agcacggcgg cgtcggccat cgcttcgata aacataaata
     1501 cgtaggctgg cgaagaaccg ctcacaccga ccaccgggtg gatcatcggc tcagcaatta
     1561 cttccgcttc gccaaagcag cggaaaatat tcagcacatc agcggtatct tctggggtta
     1621 ccagcgcgtt tggcgttacg gaggtcatcc cggcattaac cagcgcggga gtgttcggca
     1681 tggcgcggat aattttccgg tcatggccca gcgcgcgggc aagctggtcg agcgtgacac
     1741 ctgcagcaat agaaacgacc agagagtctt tattcaggct ggaggtgatt tcgctaagca
     1801 ctttaatcat gatgccaggt ttaacggcag caaaaatgat gtcggcgatt tgcgccactt
     1861 cttgcgccga ttctgcggcg ttgatgccga actggtcatg cagggcggcg actttatccg
     1921 gggagggggt gtatacccag atttgccctg gaagcacctg accgctggca atcagaccgc
     1981 cgagaatggc ttttcccata ttgccgcagc caataaaacc gattttcttt tccattgcct
     2041 cactcctgcc gtgaaattca ttgttttgat aatcgctggc agaagcataa acagaactat
     2101 gccggaaggc aaaagcgcga cacaatagag gattacctaa caaaggatga ctttatgaca
     2161 atttgggtgg atgccgacgc gtgtcccaat gtaattaaag agattttgta tcgcgcggcg
     2221 gaacgtatgc agatgccgct ggtactggta gcaaaccaga gtttacgcgt gccgccatcg
     2281 cgatttattc gtacgctgcg cgtcgcggca ggtttcgacg ttgccgataa cgaaattgtc
     2341 cggcagtgtg aagcgggcga tttggtgatc accgcagata tacctttggc tgctgaagcc
     2401 atcgagaaag gcgcagcggc gcttaattcg cgcggcgaac gttacacgcc agcgaccatt
     2461 cgtgagtgcc tgacgatgcg cgattttatg gataccttac gtgccagtgg gatccagacc
     2521 ggcggaccag atagcctttc acaacgtgac cgccaggcct ttgccgcgga gctggagaag
     2581 tggtggctgg aagtgcaacg tagtcgtggc taaatgtaat ttattattta cacttcattc
     2641 ttgaatattt attggtatag taagggatgt attgagattt ttactttaag tggaattttt
     2701 tctttacaat cgaaattgta ctagtttgat ggtatgatcg ctattctcat gacaccggct
     2761 ttcgccgcat tgcgacctat tggggaaaac ccacgatgac acaacctctt tttctgatcg
     2821 ggcctcgggg ctgtggtaaa acaacggtcg gaatggccct tgccgattcg cttaaccgtc
     2881 ggtttgtcga taccgatcag tggttgcaat cacagctcaa tatgacggtc gcggagatcg
     2941 tcgaaaggga agagtgggcg ggatttcgcg ccagagaaac ggcggcgctg gaagcggtaa
     3001 ctgcggcatc caccgttatc gctacaggcg gcggcattat tctgacggaa tttaatcgtc
     3061 acttcatgca aaataacggg atcgtggttt atttgtgtgc gccagtatca gtcctggtta
     3121 accgactgca agctgcaccg gaagaagatt tacggccaac cttaacggga aaaccgctga
     3181 gcgaagaagt tcaggaagtg ctggaagaac gcgatgcgct atatcgcgaa gttgcgcata
     3241 ttatcatcga cgcaacaaac gaacccagcc aggtgatttc tgaaattcgt agcgccctgg
     3301 cacagacgat caattgttga ttttagagcg cctatactta acgttcatcc cgtgaaataa
     3361 ggaagaacga tgccaacgaa accgccttat cctcgtgaag catatatagt gacgattgaa
     3421 aaaggaaagc caggacagac ggtaacctgg taccaactca gagccgatca tcctaaacca
     3481 gactcgttga tcagtgaaca tccgacggct caggaagcga tggatgcgaa aaaacgctat
     3541 gaggaccctg acaaagagtg accgcatcag actgctcgga agggattctg agtgccacta
     3601 caagggatct gcgtcacatt tttcataatt tatgtttttc taataattag aatattaaac
     3661 aataacattc cattactgga atcatttgga atctttacat tatgccgtgc acgtctgctg
     3721 ctacgctttt tgtcatttgt agcacaagta agtgtcagca gtggtgcttc acacttgccc
     3781 ggtaattaac gacgaaagaa aagtaaggtg gatgaacaat gagtgcgtcg ttggcgatcc
     3841 tgaccatcgg cattgtacct atgcaggagg ttttgccgct cctgacggaa tacattgacg
     3901 aagataatat ttcccatcat agcctgctgg ggaagttaag tcgtgaagaa gtgatggcgg
     3961 agtacgcgcc agaagcaggc gaagacacca ttctcacatt attaaatgac aaccagctgg
     4021 cccatgtttc gcgtcgtaaa gtggagcgag atctgcaagg tgtggttgaa gtgctcgata
     4081 atcagggtta tgacgtcatt ttattaatga gtacagcaaa cattagtagt atgactgcgc
     4141 gtaatacgat ctttcttgag ccgtcgcgaa tattgcctcc actggtttcc tctattgttg
     4201 aagatcatca ggtgggggtt atcgttccgg ttgaggagat gctgcccgtt caggcgcaaa
     4261 aatggcaaat tttgcagaaa tcgccggtat tttcattggg taaccccatt catgattcag
     4321 aacaaaaaat cattgatgct gggaaagaat tactggcaaa aggggctgat gtcatcatgc
     4381 tggattgttt aggatttcac caacgtcatc gcgatttact gcaaaaacag ctcgatgttc
     4441 ctgtcttgct ctctaacgta ttgattgcac ggctggctgc ggaattactg gtgtaatttt
     4501 gcgtgacagc cagcgcctct ggcccctata gtgaagtaga tgttcaacta ccagacaggg
     4561 ccagtttatg cttcaaagta atgagtactt ttccggcaaa gtgaaatcga tcggcttttc
     4621 cagcagcagc actggtcgcg ccagcgtggg tgttatggtt gaaggcgaat acaccttcag
     4681 caccgctgag ccggaagaga tgacggtaat cagtggcgcg ctgaatgtgt tactgcctga
     4741 cgcgaccgac tggcaagtgt atgaagccgg ttcggtgttt aatgttcccg gtcacagtga
     4801 gtttcatctt caagttgccg aacctacctc ttatctgtgc cgctatctgt aattcctcgc
     4861 cttccccttg aacgggaggg catttttctg aaatatcctc tctttaaccc ataataatat
     4921 ttcctttgct gcgatttttt caatttccga tatattcata attcatcaag gttgatataa
     4981 atatcagtga agatctccag gtattttttg cggaactggc tacgataaaa gataaatcag
     5041 atgatgaatg gtggcgtgca ttgctgcaaa gtcgcaatga tcatttgcgc caaacggcat
     5101 tacgcaacgc acataccccg gcgtcgttgt taacgacatt gactgagcct caggatcggt
     5161 cgctggctat taataatcca cagctggctg ccgatgtgaa aacggcgtgg ttaaaagagg
     5221 atccatcatt actcttattt gtcgaacaac ccgatctttc gcagttacgt gatttagtga
     5281 aaaccggggc aacgcggaaa attcgcagtg aagcgcgtca ccggcttgag gaaaaacaat
     5341 gacacaacgt ccatggagca agttacagcg ggagatctat gacttactga cgccaacgat
     5401 caacctgcaa attcactgta cccgttatcc catgcgcagc caaaatggtg gaagtaccga
     5461 tttaccgcgt tattggatca cgttggataa aaacgtgatc tgggattacc cgaaagactt
     5521 tattgcaggt aacgctggag tgcgtaattt tcacggtgaa acttgctggt atccttacct
     5581 gacggatatt tgttcaattt ctgatttatt gcgtgaatat attgataccc caaaagcgga
     5641 gctgttaaca aaacaattta ccagcgataa atgggggtta gtgaatattc tgcgtgctgc
     5701 tgacaggcga atagggatga ggcgattaga tcagttacgg cgtaaaacgc ataatattgc
     5761 tgcgctaaaa attattgctc gccgtagcga ataattatat gcctggtgtg gcttcgtacg
     5821 ccggataaga cgcggcaggc gtcgcatccg gcattaaagg aaaatcagca attaacgttg
     5881 tgcttcgcca cctaatcctt caatcaggtt ttgaattaac gctgccagtt caccagtcat
     5941 caggatgaaa tcggcatcaa aacgctgggc gaaatcttca cggtcgatat cttcgttttg
     6001 atcgcgcagc tcgtcgcaga acttaagacg cttgagcgaa ccatcgtcgc acatcacaaa
     6061 ctgaatgcgc tgctgccagt cgagcgccag tttagtcacc acttttccgg cttcaatgtg
     6121 attggtgatc tcttcgctgg tcagatcttg tttcttcgcg cggatcacgc cgccatcttc
     6181 cagcaacgat ttcagctcgg cttcatcaag cagctggaag ccctgtgccg cactaccgga
     6241 gcgaacccat tcggtcagcg tcagttcaat cgggttttcc atgctcaatg gtacaaccgg
     6301 taacgacccc aggcttttac gcagtaatgc cagcgtatct tccgcttttt tggcactggc
     6361 gcagtccacc ataatcaaac cgttaaccgt gtcgagccac atcattgtct ggctaaaacg
     6421 gctgaaagca cgcggcagca gagagtgcag cacttcgtct ttcagcgaat ctttttcggt
     6481 tttcttcagc ttacgcgcct gttccgcttc cagtttggcg attttcgctt ccagcgcctg
     6541 tttaatcacc ggagacggga ggattttttc ttctttgcgc gcgcagataa caatttgacc
     6601 attggcaacg tgcgttaacg catcgctgtg cgatcccatc ggaggaaccc agcccatctt
     6661 cgccatgtcc tggctgccgc atggggtaaa tgccatcgag gctagctgtt tttccatctc
     6721 ttctgcacgc agcgaaatct cgcggctaag acggtaaacc attaaatttt tgaaccacag
     6781 catgataatt tccacggcct tgtcgttaaa tttagcgggc atgataacga attgtcggcg
     6841 gccttgcatt gccaatccgg ttgtccgtct ctacgctatt gatattgaaa aaaataagga
     6901 gagtacagtg cgtataggta tcgatttagg cggcaccaaa actgaagtga ttgcactggg
     6961 cgatgcaggg gagcagttgt accgccatcg tctgcccacg ccgcgtgatg attaccggca
     7021 gactattgaa acgatcgcca cgttggttga tatggcggag caggcgacag ggcagcgcgg
     7081 aacggtaggt atgggcattc caggttcgat ttcgccctac accggtgtgg tgaagaatgt
     7141 caattcaacc tggctcaacg gtcagccatt cgataaagac ttaagcgcga ggttgcagcg
     7201 ggaagtgcgg ctggcaaatg atgccaactg tctggcggtt tcagaagcag tagatggcgc
     7261 ggcagcggga gcgcagacgg tatttgccgt gattatcggc acgggatgcg gcgcgggcgt
     7321 ggcattcaat gggcggacgc atatcggcgg caatggcacg gcaggtgagt ggggacacaa
     7381 tccgctaccg tggatggacg aagacgaact gcgttatcgc gaggaagtcc cttgttattg
     7441 cggtaaacaa ggttgtattg aaacctttat ttcgggcacg ggattcgcga cggattatcg
     7501 tcgtttgagc ggacatgcgc tgaaaggcag tgaaattatc cgcctggttg aagaaagcga
     7561 tccggtagcg gaactggcat tgcgtcgcta cgagctgcgg ctggcaaaat cgctggcaca
     7621 tgtcgtgaat attctcgatc cggatgtgat tgtcctgggg ggcgggatga gcaatgtaga
     7681 ccgtttatat caaacggttg ggcagttgat taaacaattt gtcttcggcg gcgaatgtga
     7741 aacgccggtg agtaaggcga agcacggtga ttccagcggc gtacgcggcg ctgcgtggtt
     7801 atggccacaa gagtaaaaaa cgtaggcaat tggcgcatca tgcctgatgc gacgcttgcc
     7861 gcgtcttatc aggcctacaa aatgtgccag aaccgcgtag ggcggataag gcgttcacgc
     7921 cgcatccggc aataagcaac gcgcaccgtg acagtaatct accccagtgg tttcgccagc
     7981 accggactat ccgccgcttg ctggcgctta tagcgaccat acagcagcaa cgatgacatc
     8041 gcagcaaacg aaagcagggc ggcaggcagc gccacgtaat tatatgccag ccccagcgtc
     8101 agcatcatac ctccgcaata tgcgccgacg gcgctaccga ggttaaacgc tatttgccca
     8161 cctgcggcac ctaataactc tcctcctttg gcattttgca gtaacaatat ttgcagaggt
     8221 gctgaaaggg caaataatcc cgcgcaacaa ataaaagcaa aaataagcga cgttgttttc
     8281 atgccgccgc agaaaaagag catcagcagt gccagtacaa tgataaagtc agtcactgct
     8341 gcaatgcgca gtggtgaata acgtcctgaa atcctgccac ttagcatatt tcccagcacc
     8401 atccctagcc caactaacat cataataaag gtcatcgccg tttccgaaaa accggaaata
     8461 aacatcatgt atggctttac gtagctgaac caggcaaaca cacctgcgtt gccaaacatc
     8521 gtggcggcga aaattaacca cggggccggg ctgcgcaaaa agtgaaattg ttcgcgcaga
     8581 tttcctttcg cctcgtcgcg aatatctggc acccaaaaat agaccgatgc catcaccgca
     8641 atattaaaaa cagcgatcaa taaaaaggtg taacgccagc attcctgact taaatacgtt
     8701 cccagcggaa tgcccagcaa attggcgact gtcatcccgg aaaccatccc cgccacggcg
     8761 gcggtgactt ttccgggttt gataattttt gataacacga tcgctccgac gccaaaaaat
     8821 gcgccatgcg gaaagccgga taccagccga ccaatggcga gcatcaggta agacgaagag
     8881 agcgtgaaca tggcgttgcc aatgacgcac aacgccacca gaaacaacaa gatatgtttg
     8941 agtgagtagc ggctggaaaa gagtgcgatg attggcgcac cgaccaccac caccagtgca
     9001 taatacgaga tcatatgccc ggcagcagga atcgaaattc ctacgttatg cgccagctcc
     9061 gtgagcacgc tcataatgcc aaattcggcc atccccaaac caaacgtgcc cagagccaaa
     9121 gataaaatga cttttttcat accaccatca caaccaccag aacggctacc aatcaccgta
     9181 gccaataaag tgatagcttg caggataacg accagcaagg ccaagagata attcacccct
     9241 ctgtattcat tatcctgctg aatagttatt tcactgcaaa cgtactttcc agtttgctgt
     9301 agcccaggcc gttgatcttt ttcactttga tctgcaccgg aatacgctct ttcatcgctt
     9361 ctacgtggct aatcacaccg atggttttgc cactggcgtt cagggcatcc agcgcatcaa
     9421 gggcggtatc cagcgtttcg ctatccagcg tgccaaaacc ttcatcaagg aacagcgagt
     9481 caatacgtgt tttatggctg accagatccg aaagcgccag cgccagcgcc agactaacga
     9541 ggaaactttc gccgccggaa agggtacggg tatcgcgtac cgcatctgcc tgccaggtat
     9601 caacaacctc gacttccagc gcctcgctgg ctttgcgctg taacagatag cgcacgtgca
     9661 gccgggtaag ttgctgatta gcgagatgga ctaaattatc cagcgtcagc ccctgggcaa
     9721 acttgcggaa tttatcgccc tctttggaac ctattagcga attcagatat ccccagtcct
     9781 caacctgctg cgtcatttga gcaatttgct gcattaaggt ttgttgttgc tgacggttat
     9841 ctgcatcctg cttcagctgc tggcgaatct cgccttgact cgtggtgttt tcacgcaact
     9901 tttggtgagt ttgcgctaac tcttgctgaa tctgctccac cgtcacagtg agagccaacc
     9961 cgtcaggtcg gtgttgttga tgctgtgcca gcgtttctgc tgtctgagtg accagcgttt
    10021 gcgcctgacg gcgctggttt tccagattct gcttgagctg ttccagctgc gttagtgttt
    10081 gttcatccat tagcgccgca aggaacgcct gctgatcgtc aaagacgctg gcctgtagcg
    10141 cggtgtcaaa ctgcgcttgg gctttttgca gactttgcgc cgccagaaca tcctgttgct
    10201 gtaacgtctg ctgctggctg tgtaatgcga gacattgttc atgaacctgc cgccagttat
    10261 ccagcgccac agtttcttcg ctgtgcggga gatcatcact ttgcggcaac gtttccagaa
    10321 tcggtgtcag ctgctgaata cggttttgca gcgcggttaa ttcgttctgg cgttgctgcc
    10381 agctctgcgc ttcttgctga cgtgtcgcca accagctctc ttcttcatct tcctgtggca
    10441 atgtcagtgc ataacccgcc aatgccgtta agagctgctg ctgacgttgt tcaatttgct
    10501 gttgatactg gataatttgc tgattatgcg cggcaatctg cccttgtaat tcatgtcgtt
    10561 ggctgagtaa ccgcagctgg cgttcgtgct catcttgtgc atccagccac ggttgaatat
    10621 cgtcctgtgg ctgcaaggtg atattgaggc tggccgtgac ggcttgccat tgttgagtaa
    10681 gtgcttgctc atcttgtcgg aggctttgcg cttcgttttc atcgcgctga agctgcttcg
    10741 ttaatgcatc cagttgcccg cgcagcgccg caccttcttc gccgagcttt ttaacttcgt
    10801 tttccagcgc cagtaatcga gactgattaa caccaggctc cagcgcctga tacgcctcga
    10861 ccgccgggtg gctggtggaa ccacaaagtg gacaaggctg acccgcctgt aactgtgcac
    10921 gctgggcttc cagcgttttg atgcgcgctt cctgctcgca aatggttttc acatcggcaa
    10981 gttgctgcgt cttttcttta taacgctggc gcatttcgtt aagtgcgaca ttacgttgcg
    11041 tctgttccag agtgacattc tggatagtga ccattaactg cgccagacgt ttttgttggg
    11101 gaacaatctg cccatgcagc gcgaccaggc gctgacgcag tgggcgttgc tcggcatgtt
    11161 gcgccagggc ggtagcaact tcatcggcgg ttaacgtcaa cgtgatcgcc gcaagcgcat
    11221 taagtttttg ctcagcatgg gttaactgtt gctgccattg ccgcagatgc tcgcgatcgc
    11281 tggtttgttg ggagaactgc gcacgccaac ccgccagttc gttgttccac tgacggaagc
    11341 ggtcgtgttc ctgtaaccag gtattcaggc tttgttgctg ctgctgtaat tctgctgact
    11401 gcttcgccgc gtggtggcga atgctcgcgc gaagcgccat tgtgctctgt aagcgagtat
    11461 ttacttcttc aatctgctgg cgaatatgcg ccagcgccgc gctgtgttct gcgatgcgtt
    11521 cccagtgtgg acgaagattt cgtgccggtt gtgccagact aagcgccgcc agttgaggtt
    11581 gcgctttttc ttcttcggct aacgcctgtt gcaaggcctg ttgacggcgg ctggcttctt
    11641 gctgcaattc gtcctgacgc gttaaccagt ttagcgattg ttgttcttgc tgctgcgcgg
    11701 taattaactg tttttcttcg tcagtaagta cctgcaaact cgctgtcagc gattgcactt
    11761 gttccggcgt gagcaacgtg acgccgctgg cctgcgcttg cagcttctcc agctcagtgc
    11821 gggccgattt gtgctgctca aaaaccatcg ccgagatttg cccgtagatt tcagtgccgg
    11881 ttaactcctc gagcaattcc gcgcgttctt tgggtttggc attcaggaag gcagcaaatt
    11941 gcccctgcga aagcagcatc gaacgggtga agcgcccgta atccagcccg gttaacgtcg
    12001 ctgtcagttc cagcttatct ttcactttgt cggcgagaat tttgccgtcg gcgcagcgcg
    12061 ccagctctac gcgaggcacc tgcaaattac cgtccggctg gttacgcgcc cgattctggc
    12121 tccagaacgc gcgatacgct tcacctttca cttcaaactc cacctccgcc agacattcag
    12181 cggtatcgcg ggtcatgaga tcattttgcg attgtgaaac gttagagaga cgcggagttt
    12241 cgtgatacag cgccagacaa atggcgtcca gcagggtggt tttccccgca ccggttgggc
    12301 cggtaatggc aaacagcccg ttgctggcga acggctcgcg ggtgaaatca atcttccatt
    12361 cgccttttaa tgagttcagg tttttcaggc gcaggctgag aattttcatg cttcgtgttc
    12421 tccggcgagg gtacgcaacg tcgtggtgaa aagatgctgc agacgttgct gctgcgattc
    12481 atccaggtct tccagtgcca gacggcgatt gaacacctct tcgacgctga gttcgctgag
    12541 ggtttcacgc tgttggctgg ctaacacacg ctcgcgctgt tcacgactcc gacgtaccag
    12601 caatacttcg acaggcaatg attcggttaa tgcctggatt ttgcgctgaa tatcatgcag
    12661 atactcatca gtagtgattt cgatatccag ccagacaggt ggctcctgcg atacatcgcg
    12721 ccactgttcc agctgtgcgg taatcgacgc cagatcgcct ttcagcaccg ccatgggttg
    12781 agttaccggt acgttcaggt tttccacgct ctctaatttg ccgtttgaaa atgtcaccag
    12841 atggacatat ttactcttac cgcattcatc aaaactcagt ggaatggggg aaccgcaata
    12901 gcgaacatgt tccatgccgc caataatctg tgcgcggtga atatgcccga gcgcgatgta
    12961 gtcggctggt ggaaagtttt gtgccggaaa cgcgtccagc gtgccaatat aaatgtcacg
    13021 cacggcgtca cttttactgg aacccacggt cgttaaatgt cccgtggcga tgatgggcag
    13081 aggctgatcg ccgcgcagtt tgcaggcatc ggcatagtgt tgttggtaat aatcggtaat
    13141 cgctgccagt aaatgctgct gtttttcaat accgttaagc cccgcctggc tggaaataat
    13201 gtcacgcggg cgtaaaaacg gaatggggca cagcactgcg cctggcgtcc cgtcgcgacg
    13261 aggcaagatt tgcggcgcat gtccggcgct ggcgaccacg gttgtattga ggaacgccat
    13321 gatatcgcgc gattcattca gcgtggcgac cgagtcatgg tttcctgcca gtaccaccag
    13381 atgacagcca gtttgctgta aattgacaac aaaacggttg tataacgtgc gggcgtaact
    13441 gggcggcgag ccggtatcga aaacatcacc agcaacaata atcgcatcca cctgatgggt
    13501 ttgtgctgtc tccagcagcc agtcaagaaa agcctgatgt tcagcttcgc ggcttttact
    13561 gtagaagttc tggccgagat gccagtctga ggtgtgaagg atgcgcataa cggttccctg
    13621 gcgaaaaagc atggtcgcga ttatacccaa acagatgtgc catttgcttt tttctgcgcc
    13681 acggaaatca ataacctgaa gatatgtgcg acgagctttt cataaatctg tcataaatct
    13741 gacgcataat gacgtcgcat taatgatcgc aacctattta ttacaacagg gcaaatcatg
    13801 gcgagacgta ttctggtcgt agaagatgaa gctccaattc gcgaaatggt ctgcttcgtg
    13861 ctcgaacaaa atggctttca gccggtcgaa gcggaagatt atgacagtgc tgtgaatcaa
    13921 ctgaatgaac cctggccgga tttaattctc cttgactgga tgttacctgg cggctccggt
    13981 atccagttca tcaaacacct caagcgcgag tcgatgaccc gggatattcc agtggtgatg
    14041 ttgaccgcca gaggggaaga agaagatcgc gtgcgcggcc ttgaaaccgg cgcggatgac
    14101 tatatcacca agccattttc gccgaaggag ctggtggcgc gaatcaaagc ggtaatgcgc
    14161 cgtatttcgc caatggcggt ggaagaggtg attaagatgc agggattaag tctcaacccg
    14221 acatctcacc gagtgatggc gggcgaagag ccgctggaga tggggccgac agaatttaaa
    14281 ctgctgcact tttttatgac gcatcctgag cgcgtgtaca gccgcgagca gctgttaaac
    14341 cacgtctggg gaactaacgt gtatgtggaa gaccgcacgg tcgatgtcca cattcgtcgc
    14401 ctgcgtaaag cactggagcc cggcgggcat gaccgcatgg tgcagaccgt gcgcggtaca
    14461 ggatatcgtt tttcaacccg cttttaacgc cttgctcatc ggacgcagag cagggcttat
    14521 gatttcttaa ctggagtatc ttacgtgctg gaacggctgt cgtggaaaag gctggtgctg
    14581 gagctgctac tttgctgcct cccggctttc atcctgggtg cattttttgg ttacctgccc
    14641 tggtttttgc tggcatcggt aacaggactg cttatctggc atttctggaa tttattgcgc
    14701 ctttcatggt ggctgtgggt ggatcgcagt atgaccccgc caccggggcg tggtagctgg
    14761 gaaccgctac tatacggctt acaccagatg cagctgcgaa ataaaaaacg ccgccgtgaa
    14821 ctgggcaatc tgattaaacg ctttcgtagc ggcgcggagt cgctgcccga cgcggtggtg
    14881 ctgaccacgg aagagggcgg tattttctgg tgtaacggtc tggcgcaaca aattcttggt
    14941 ttgcgctggc cggaagataa cgggcagaac atccttaacc tactgcgtta cccggagttt
    15001 acgcaatatc tgaaaacgcg tgatttttct cgcccgctca atctggtgct caacaccggg
    15061 cggcatctgg aaattcgcgt catgccttat acccacaaac agttgctgat ggtggcgcgt
    15121 gatgtcacgc aaatgcatca actggaaggg gcgcggcgta acttttttgc caacgtgagc
    15181 catgagttac gtacgccatt gaccgtgtta cagggttacc tggagatgat ggatgagcag
    15241 ccgctggaag gcgcggtacg cgaaaaagcg ttgcacacca tgcgcgagca gacccagcgg
    15301 atggaagggc tggtgaagca gttgctaacg ctgtcgaaaa ttgaagccgc gccgacgcaa
    15361 ttgctcaatg aaaaggttga tgtgccgatg atgctgcgcg ttgttgagcg cgaggctcag
    15421 actctgagtc agaaaaaaca gacatttacc tttgagatag ataacggcct caaggtgtct
    15481 ggcaacgaag atcagctacg cagtgcgatt tcgaacctgg tctataacgc cgtgaatcat
    15541 acgccggaag gcacgcatat caccgtacgc tggcagcgag tgccgcacgg tgccgaattt
    15601 agcgttgaag ataacggacc gggcattgca ccggagcata ttccgcgcct gaccgagcgt
    15661 ttttatcgcg ttgataaagc gcgttcccgg caaaccggcg gtagcggatt agggttagcg
    15721 atcgtgaaac atgcggtgaa tcatcacgaa agtcgcctga atattgagag tacagtagga
    15781 aaaggaacac gtttcagttt tgttatcccg gaacgtttaa ttgccaaaaa cagcgattaa
    15841 tccgcctttg tcatctttta ttgccataag ccagccgatg ctggcttatt ttctttgcag
    15901 tcaaaatacg ggcgttagat tttacaacgg ttggtgattt tttgttcgca tgattagcca
    15961 tgtctttttc acggaaatag tgttttatac tggttggtga tttcttatcg ctatatacct
    16021 ctggttttta gatccctcct tgctttaaaa cgttataagc gtttaaattg cgcttcaggt
    16081 gctgtcatac tgactgcatt aacgcggtaa atcgaaaaac tattcttcgc cgcgcctggt
    16141 tgggagtatt tcccgctaaa attgtttaaa tataccgctg tatcatcccc agggattggc
    16201 acaaaaattt aacgttacaa caccacatcc acaggcagta tgatttatga cccatcaatt
    16261 aagatcgcgc gatatcatcg ctctgggctt tatgacattt gcgttgttcg tcggcgcagg
    16321 taacattatt ttccctccaa tggtcggttt acaggcaggc gaacacgtct ggactgcggc
    16381 attcggcttc ctcattactg ccgttggcct gccggtatta acggtagtgg cgctggcaaa
    16441 agttggcggc ggtgttgaca gcctcagcac gccaatcggt aaagtcgctg gcgtactgct
    16501 ggcaacggtt tgttacctgg cggtggggcc gcttttcgct acgccgcgta cagctaccgt
    16561 ttcctttgaa gtggggattg cgccgctgac gggtgattcc gcgctgccgc tgtttatcta
    16621 cagcctggtc tatttcgcta tcgttattct ggtttcgctc tatccgggca agctgctgga
    16681 taccgtgggc aacttccttg cgccgctgaa aattatcgcg ctggtcatcc tgtctgttgt
    16741 cgcgattgtc tggccggcgg gttctatcag cacggcgact gaggcttatc aaaacgctgc
    16801 attttctaac ggcttcgtta acggctatct gaccatggat acgctgggcg caatggtgtt
    16861 tggtatcgtt attgttaacg cggcgcgttc tcgtggcgtt accgaagcgc gtctgctgac
    16921 ccgttatacc gtctgggctg gcctgatggc gggtgttggt ctgactttgc tgtacctggc
    16981 gctgttctgt ctgggttcag acagcgcgtc gctggtcgat cagtctgcaa acggcgctgc
    17041 tattctgcat gcttacgttc agcacacctt tggcggcggc ggtagcttcc tgctggcggc
    17101 gttaatcttc atcgcctgtc tggtcacggc ggttggcctg acctgtgctt gtgcagaatt
    17161 ctttgcccag tacgtaccgc tctcttatcg tacgctggtg tttatcctcg gcggcttctc
    17221 gatggtggtg tctaacctcg gcttaagcca gctgatccag atctccgtac cggtgctgac
    17281 cgctatttat ccgccgtgta tcgcactggt tgtattaagt tttacacgct catggtggca
    17341 taattcgtcc cgcgtgattg ctccgccgat gtttatcagc ctgctttttg gtattctcga
    17401 cgggatcaaa gcatctgcat tcagcgatat cttaccgtcc tgggcgcagc gtttaccgct
    17461 ggccgaacaa ggtctggcgt ggttaatgcc aacagtggtg atggtggttc tggccattat
    17521 ctgggatcgc gcggcaggtc gtcaggtgac ctccagcgct cactaaatca ctgaacattt
    17581 gttttaacca cggggctgcg atgccccgtg gttttttatt gtgttgatgg gttaggaatt
    17641 gatggaaagt aagaataagc taaagcgtgg gctaagtacc cgccacatac gctttatggc
    17701 actgggttca gcaattggca ccgggctgtt ttacggttcg gcagacgcca tcaaaatggc
    17761 cggtccgagc gtgttgttgg cctatattat cggtggtatc gcggcgtata tcattatgcg
    17821 tgcgctgggg gaaatgtcgg tacataaccc ggccgccagc tctttctcgc gttatgcgca
    17881 ggaaaacctt ggcccgctgg caggttacat taccggatgg acctactgtt ttgaaatcct
    17941 tattgtcgcc atcgccgatg tgaccgcttt tggtatctat atgggtgtct tgttcccgac
    18001 ggtgccgcac tggatttggg tactgagcgt ggtgctgatc atttgcgccg taaacctgat
    18061 gagcgtgaag gtcttcggtg agctggaatt ctggttctcg ttctttaaag tcgccaccat
    18121 catcatcatg attgtcgccg gtttcggcat catcatctgg gggattggca acggcgggca
    18181 accgaccggt attcataacc tgtggagcaa cggcggcttc ttcagtaacg gctggcttgg
    18241 catggtaatg tcgttgcaaa tggtgatgtt tgcttacggt gggatcgaaa ttatcgggat
    18301 taccgccggt gaagcgaaag atcctgagaa atcgattccg cgtgcgatta actccgtgcc
    18361 gatgcgtatt ctggtgttct acgtcggtac gctgttcgtc attatgtcta tctacccgtg
    18421 gaatcaggtt ggcactgccg gtagcccgtt cgtactgacg ttccagcata tgggcattac
    18481 ctttgccgcc agcattctta actttgttgt gctgaccgct tcgctgtcgg caattaacag
    18541 tgacgtattt ggcgtaggcc gtatgctcca cggtatggca gagcagggca gcgcaccgaa
    18601 aattttcagc aaaacgtcgc gtcgcggtat tccgtgggtt acggtgctgg tgatgactac
    18661 cgcgctgctg tttgcggtgt atctgaacta catcatgccg gaaaacgtct tcctggtgat
    18721 cgcttcgctg gcaaccttcg ccacggtatg ggtgtggatt atgatcctgc tgtcgcaaat
    18781 cgccttccgt cgccgtttgc cgccagaaga agttaaggcg ctgaaattta aagtgccggg
    18841 tggggtagca acgaccatcg gcgggctgat tttcctgctc tttattatcg ggttgattgg
    18901 ttatcacccg gatacgcgta tctcgctgta tgtcggtttc gcgtggattg ttgtgctgtt
    18961 gattggctgg atgtttaaac gccgccacga tcgtcagctg gctgaaaacc aataatactt
    19021 gcgttctgta agccggttca ggaggaaatc ctgatccggc tattccgaaa gttatccgcc
    19081 cccgtcctcc tcccccaaat atccttcaga tgatgagtga tcctgcatta ggctatggca
    19141 aggtgatcag attttcatca caggggaatt atgatgttaa atgcatggca cctgccggtg
    19201 cccccatttg ttaaacaaag caaagatcaa ctgctcatta cactgtggct gacgggcgaa
    19261 gacccaccgc agcgcattat gctgcgtaca gaacacgata acgaagaaat gtcagtacca
    19321 atgcataagc agcgcagtca gccgcagcca ggcgtcaccg catggcgtgc ggcgattgat
    19381 ctctccagcg gacaaccccg gcggcgttac agtttcaaac tgctgtggca cgatcgccag
    19441 cgttggttta caccgcaggg cttcagccga atgccgccgg cacgactgga gcagtttgcc
    19501 gtcgatgtac ctgatatcgg cccacaatgg gctgcggatc agatttttta tcagatcttc
    19561 cctgatcgtt ttgcgcgtag tcttcctcgt gaagctgaac aggatcatgt ctattaccat
    19621 catgcagccg gacaagagat catcttgcgt gactgggatg aaccggtcac ggcgcaggcg
    19681 ggcggatcaa cgttctatgg cggcgatctg gacgggataa gcgaaaaact gccgtatctg
    19741 aaaaagcttg gcgtgacagc gctgtatctc aatccggtgt ttaaagctcc cagcgtacat
    19801 aaatacgata ccgaggatta tcgccatgtc gatccgcagt ttggcggtga tggggcgttg
    19861 ctgcgtttgc gacacaatac gcagcagctg ggaatgcggc tggtgctgga cggcgtgttt
    19921 aaccacagtg gcgattccca tgcctggttt gacaggcaca atcttggcac ggggggagct
    19981 tgtcacaact ctgaatcgcc ctggcgcgac tggtactcgt ttagtgatga tggcacggcg
    20041 ctcgactggc ttggctatgc cagcttgacg aagctggatt atcagtcgga aagtctggtg
    20101 aatgaaattt atcgcgggga agacagtatt gtccgccact ggctgaaagc gccgtggaat
    20161 atgga
//
LOCUS       DAZXUK010000046        20120 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZXUK010000046 DAZXUK010000000
VERSION     DAZXUK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20120
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.046"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="ACH3L8_001369"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="ACH3L8_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFT4084546.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1102..1185
                     /gene="yqiM"
                     /locus_tag="ACH3L8_001370"
     CDS             1102..1185
                     /gene="yqiM"
                     /locus_tag="ACH3L8_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFT4084547.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1207..1869
                     /gene="yqjA"
                     /locus_tag="ACH3L8_001371"
     CDS             1207..1869
                     /gene="yqjA"
                     /locus_tag="ACH3L8_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFT4084548.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="ACH3L8_001372"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="ACH3L8_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFT4084549.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /gene="yqjC"
                     /locus_tag="ACH3L8_001373"
                     /pseudo
     CDS             2403..2757
                     /gene="yqjC"
                     /locus_tag="ACH3L8_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
     gene            2795..3100
                     /locus_tag="ACH3L8_001374"
     CDS             2795..3100
                     /locus_tag="ACH3L8_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFT4084550.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="ACH3L8_001375"
     CDS             3103..3507
                     /locus_tag="ACH3L8_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFT4084551.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="ACH3L8_001376"
     CDS             3497..3796
                     /locus_tag="ACH3L8_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFT4084552.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="ACH3L8_001377"
     CDS             3982..4278
                     /locus_tag="ACH3L8_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFT4084553.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="ACH3L8_001378"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="ACH3L8_001378"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFT4084554.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="ACH3L8_001379"
     CDS             5627..5995
                     /locus_tag="ACH3L8_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFT4084555.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="ACH3L8_001380"
     CDS             complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="ACH3L8_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFT4084556.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7203..7904
                     /locus_tag="ACH3L8_001381"
     CDS             7203..7904
                     /locus_tag="ACH3L8_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFT4084557.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7927..8091
                     /gene="yhaL"
                     /locus_tag="ACH3L8_001382"
     CDS             7927..8091
                     /gene="yhaL"
                     /locus_tag="ACH3L8_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFT4084558.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8303..9613)
                     /gene="cyuA"
                     /locus_tag="ACH3L8_001383"
     CDS             complement(8303..9613)
                     /gene="cyuA"
                     /locus_tag="ACH3L8_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFT4084559.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9641..10972)
                     /locus_tag="ACH3L8_001384"
     CDS             complement(9641..10972)
                     /locus_tag="ACH3L8_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFT4084560.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="ACH3L8_001385"
     CDS             complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="ACH3L8_001385"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFT4084561.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12683..13072)
                     /locus_tag="ACH3L8_001386"
     CDS             complement(12683..13072)
                     /locus_tag="ACH3L8_001386"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFT4084562.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="ACH3L8_001387"
     CDS             complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="ACH3L8_001387"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFT4084563.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="ACH3L8_001388"
     CDS             complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="ACH3L8_001388"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFT4084564.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="ACH3L8_001389"
     CDS             complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="ACH3L8_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFT4084565.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="ACH3L8_001390"
     CDS             complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="ACH3L8_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFT4084566.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="ACH3L8_001391"
     CDS             complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="ACH3L8_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFT4084567.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         5204 a         5270 c         4811 g         4835 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtaga gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc
     6661 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     6721 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     6781 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     6841 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     6901 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     6961 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc
     7021 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     7081 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     7141 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     7201 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     7261 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     7321 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     7381 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     7441 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     7501 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     7561 atacctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc
     7621 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt
     7681 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     7741 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     7801 cggcgctaac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     7861 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     7921 atgatgatga gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa
     7981 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     8041 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     8101 gccggatgcg gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa
     8161 acaccccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     8221 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     8281 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     8341 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
     8401 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     8461 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     8521 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
     8581 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     8641 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     8701 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
     8761 ccacaggcat tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa
     8821 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     8881 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     8941 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
     9001 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
     9061 tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt
     9121 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     9181 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     9241 tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg
     9301 taattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     9361 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     9421 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     9481 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     9541 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
     9601 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca
     9661 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
     9721 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
     9781 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
     9841 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
     9901 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
     9961 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    10021 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    10081 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    10141 cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt
    10201 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    10261 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    10321 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    10381 acacgcccag cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga
    10441 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    10501 aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac
    10561 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    10621 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    10681 ctttacattc tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg
    10741 ccgggtaacc aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca
    10801 ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg
    10861 tgtcagtact gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac
    10921 ccgccggggt cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc
    10981 gctcaatatt ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc
    11041 tacgtttttg caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat
    11101 cttcacaatc aagactttaa aataacggag atggataaga attttctact aaattaatca
    11161 caagagaata ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca
    11221 cccaaggatg aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg
    11281 cgtgatgttt cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc
    11341 actttatcga gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt
    11401 actgctttca cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca
    11461 accggatcgc aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc
    11521 tgcgccggac tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg
    11581 ccaatctccc cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc
    11641 gcgccaatag cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga
    11701 cggaacttat cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt
    11761 gccgttacca cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg
    11821 atccagtcga tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga
    11881 cgcagcgcta cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc
    11941 atcccgcgtt caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt
    12001 tccgctttgc tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg
    12061 ttgtaatcac acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt
    12121 tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag
    12181 taataggttt tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc
    12241 tcatggcgag gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca
    12301 atatgcgcac cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca
    12361 gggatctcat caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg
    12421 acatccgtgg catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata
    12481 tggctcgtcg cggttaataa gccgctactt tccagccgat caataaaact ttttccggca
    12541 ttcattggcc ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat
    12601 gcactaatca tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg
    12661 cgaccgtccc gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct
    12721 tcacatcttt cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat
    12781 gctcatcgaa gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga
    12841 tgaacacggt catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt
    12901 tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct
    12961 gcgggcaaac cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa
    13021 cataagggcc gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc
    13081 cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc
    13141 agtgcgttga agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc
    13201 gggtgctcga tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga
    13261 ccgccttcga catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt
    13321 ttacgtactg gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc
    13381 ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct
    13441 ttgcggtcac gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga
    13501 ccgtccggcg tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc
    13561 gactgggtag ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt
    13621 tcaaccaagt cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat
    13681 tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg
    13741 gcatatttga tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc
    13801 atagtgcgat agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg
    13861 tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg
    13921 aagtgatcga ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct
    13981 gttttcggcc cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc
    14041 agcgttttcg ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt
    14101 gggctgacgc agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg
    14161 tcattttcat actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag
    14221 gcaatcggta attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata
    14281 gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc
    14341 cccatcccgc cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat
    14401 tccggtgtac gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc
    14461 tgtgcctgct gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg
    14521 aacgatgccg tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga
    14581 taagcgaagt agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca
    14641 aagccatatt tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc
    14701 tcctcacgca gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga
    14761 tcggcaaatt gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg
    14821 cgatagtcac cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca
    14881 gatttacggc agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt
    14941 ttacgcagat cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg
    15001 aatgaacttt taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca
    15061 tccgtttgca ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg
    15121 gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata
    15181 cggatgcctt ccattacctt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga
    15241 aaagattcat cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt
    15301 tcgcttttcc agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg
    15361 gtatcaatat ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca
    15421 aattctgcgg gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct
    15481 tcgttagtcg gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg
    15541 ccaaaagagt tagaacgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa
    15601 tgttccatga ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata
    15661 attccatcca gccgacgtaa tgaagctgca tgtccggcaa tatgacgggc aattcggtga
    15721 acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga
    15781 acacgtaaat ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg
    15841 cgttccaggt cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag
    15901 tcgacatcgc cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag
    15961 gtgtcaacac tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc
    16021 acaaccaggc cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca
    16081 tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc
    16141 cacggcaggc cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat
    16201 accgccacct gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt
    16261 gcgtaattat gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg
    16321 gtaataatgg cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag
    16381 gccacgctgt catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct
    16441 tcgtagctgt ggtgagccag cggtgctggc tctcccccat ttacggataa gaatgcattt
    16501 tccgagttaa taccgtcggc aatacctgac attaatactt cacaatcgct ggcatcgagt
    16561 acggaaaact taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc
    16621 atctcttttc tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt
    16681 cagcagacca atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga
    16741 cggcgcttta cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc
    16801 gcccatggct tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga
    16861 gcccatgatg aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc
    16921 acctttataa ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg
    16981 accgaagaaa gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt
    17041 aatcgcgaac gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag
    17101 cactggaata ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca
    17161 gctaaaggca aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg
    17221 ggaacattta cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga
    17281 aaccacgaag gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc
    17341 cagccagaca gtgatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac
    17401 ctggtcgata actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct
    17461 ggcgataaac ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca
    17521 gatgacgaaa gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc
    17581 aaatccgagc tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat
    17641 ccacagcagt gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt
    17701 accaaaatgc tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca
    17761 cagacgcgcc agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag
    17821 aagaatcggg atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc
    17881 gattgccgta ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg
    17941 ttttgtctgg ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa
    18001 ttaagcgtca acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga
    18061 aataatactg acggtttttc tattttgaat atattggtct aatttaccgc ttaataatgc
    18121 agcacatgcc agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat
    18181 catactgttt ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac
    18241 gatttcgtaa gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt
    18301 agttcggtgc gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc
    18361 tgactgtacg ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc
    18421 agcaattaaa ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc
    18481 cataatttcc agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg
    18541 gataaaaata cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa
    18601 gttatcacca tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt
    18661 tggcgcacct tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga
    18721 aaccccttgc gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc
    18781 ggtcagtgaa cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat
    18841 attttcaaac ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg
    18901 catgcctgtt ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat
    18961 agcaaccggc agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg
    19021 taggtaaccg taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa
    19081 acaaacaact aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac
    19141 tctttggcta attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat
    19201 accgcggcat attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta
    19261 gaaccaaaag gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca
    19321 agattataaa ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt
    19381 ctttgtaacg tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac
    19441 tgttcgttct tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct
    19501 accagcacaa actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct
    19561 gcacttaacg taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa
    19621 gacagttgcg cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg
    19681 atcatccctg acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc
    19741 accgcttcag aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg
    19801 gattcggaac gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc
    19861 cgcaccacta attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc
    19921 ggttgagtta accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc
    19981 tgaaagacta ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt
    20041 aattacctca ttgacggcat gaagtgtatc aaaatgaaat aaacaggata tgtgcgacca
    20101 ctcacaaatt aactttcatt
//
LOCUS       DAZXUK010000047        20011 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZXUK010000047 DAZXUK010000000
VERSION     DAZXUK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20011
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.047"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            3..935
                     /gene="ybeQ"
                     /locus_tag="ACH3L8_001392"
     CDS             3..935
                     /gene="ybeQ"
                     /locus_tag="ACH3L8_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFT4084568.1"
                     /translation="MIERAEKGDCEAQYIVGFYYNRDSAIDSPDDEKAFYWLKLAAEQ
                     GHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASNALGWILDRGEDPNYK
                     EAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYKQAALQGHSYAQDNLA
                     DLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGEGVDQDYKQAMYWYLK
                     AAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDATAWYNLAIMYHYGEG
                     RPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(1005..1487)
                     /locus_tag="ACH3L8_001393"
     CDS             complement(1005..1487)
                     /locus_tag="ACH3L8_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFT4084569.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            1722..4304
                     /gene="leuS"
                     /locus_tag="ACH3L8_001394"
     CDS             1722..4304
                     /gene="leuS"
                     /locus_tag="ACH3L8_001394"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFT4084570.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4319..4900
                     /gene="lptE"
                     /locus_tag="ACH3L8_001395"
     CDS             4319..4900
                     /gene="lptE"
                     /locus_tag="ACH3L8_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFT4084571.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            4900..5931
                     /gene="holA"
                     /locus_tag="ACH3L8_001396"
     CDS             4900..5931
                     /gene="holA"
                     /locus_tag="ACH3L8_001396"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFT4084572.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            5933..6574
                     /gene="nadD"
                     /locus_tag="ACH3L8_001397"
     CDS             5933..6574
                     /gene="nadD"
                     /locus_tag="ACH3L8_001397"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFT4084573.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6598..7209
                     /locus_tag="ACH3L8_001398"
     CDS             6598..7209
                     /locus_tag="ACH3L8_001398"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFT4084574.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7469..7786
                     /gene="rsfS"
                     /locus_tag="ACH3L8_001399"
     CDS             7469..7786
                     /gene="rsfS"
                     /locus_tag="ACH3L8_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFT4084575.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7790..8257
                     /gene="rlmH"
                     /locus_tag="ACH3L8_001400"
     CDS             7790..8257
                     /gene="rlmH"
                     /locus_tag="ACH3L8_001400"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFT4084576.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8288..10189
                     /gene="mrdA"
                     /locus_tag="ACH3L8_001401"
     CDS             8288..10189
                     /gene="mrdA"
                     /locus_tag="ACH3L8_001401"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFT4084577.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10192..11304
                     /gene="mrdB"
                     /locus_tag="ACH3L8_001402"
                     /gene_synonym="rodA"
     CDS             10192..11304
                     /gene="mrdB"
                     /locus_tag="ACH3L8_001402"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFT4084578.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11315..12403
                     /gene="rlpA"
                     /locus_tag="ACH3L8_001403"
     CDS             11315..12403
                     /gene="rlpA"
                     /locus_tag="ACH3L8_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFT4084579.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12542..13753
                     /gene="dacA"
                     /locus_tag="ACH3L8_001404"
     CDS             12542..13753
                     /gene="dacA"
                     /locus_tag="ACH3L8_001404"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFT4084580.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13864..14127
                     /gene="ybeD"
                     /locus_tag="ACH3L8_001405"
     CDS             13864..14127
                     /gene="ybeD"
                     /locus_tag="ACH3L8_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFT4084581.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14228..14869
                     /gene="lipB"
                     /locus_tag="ACH3L8_001406"
     CDS             14228..14869
                     /gene="lipB"
                     /locus_tag="ACH3L8_001406"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFT4084582.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15128..16081
                     /locus_tag="ACH3L8_001407"
     CDS             15128..16081
                     /locus_tag="ACH3L8_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFT4084583.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            16290..17255
                     /gene="lipA"
                     /locus_tag="ACH3L8_001408"
     CDS             16290..17255
                     /gene="lipA"
                     /locus_tag="ACH3L8_001408"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFT4084584.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17356..17559)
                     /gene="tatE"
                     /locus_tag="ACH3L8_001409"
     CDS             complement(17356..17559)
                     /gene="tatE"
                     /locus_tag="ACH3L8_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFT4084585.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17688..18476)
                     /locus_tag="ACH3L8_001410"
     CDS             complement(17688..18476)
                     /locus_tag="ACH3L8_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFT4084586.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            18569..18952
                     /gene="crcB"
                     /locus_tag="ACH3L8_001411"
     CDS             18569..18952
                     /gene="crcB"
                     /locus_tag="ACH3L8_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFT4084587.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(19006..19215)
                     /gene="cspE"
                     /locus_tag="ACH3L8_001412"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19006..19215)
                     /gene="cspE"
                     /locus_tag="ACH3L8_001412"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFT4084588.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(19390..19950)
                     /gene="pagP"
                     /locus_tag="ACH3L8_001413"
     CDS             complement(19390..19950)
                     /gene="pagP"
                     /locus_tag="ACH3L8_001413"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFT4084589.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5046 a         5068 c         5034 g         4863 t
ORIGIN      
        1 agattatcga acgtgctgaa aaaggcgatt gcgaggctca atatattgtt gggttttatt
       61 ataatcgcga tagcgcaatt gactctccgg acgacgaaaa ggccttttac tggctgaagc
      121 tggccgctga gcaaggtcat tgtgaagcac agtattcctt agggcaaaag tataccgagg
      181 ataaaagccg tcataaagat aatgagcaag ccatcttctg gctgaaaaaa gctgccctac
      241 aaggccatac tttcgcttcc aacgcccttg gctggatact ggatcgtgga gaagacccca
      301 actataaaga agcggttacc tggtatcaga tagccgcgga gagcggaatg tcttatgcgc
      361 aaaataatct tggatggatg tacagaaatg gcaacggagt cgcacaagac tatacgctgg
      421 catttttctg gtacaaacaa gctgcattac aaggccatag ttacgcacaa gacaatctgg
      481 ccgatcttta taaagacgga gaaggcgttg ctcaaaacaa gacactcgcc gcattctggt
      541 atttgaaaag cgcacagcag ggtaatcggc acgcccagtt tcaaattgca tgggattata
      601 acgctggcga aggggtggac caggactata agcaagcgat gtactggtat ctgaaggctg
      661 ccgctcagga aagcgtcggc gcttacgtca acatcggtta tatgtataaa cacggacaag
      721 gcgttgagaa agattatcag gctgcctttg aatggtttac gaaagccgct gaatgcaatg
      781 acgccactgc ctggtataac ctggccatta tgtatcacta cggagaagga agacctgtcg
      841 atctccgaca ggctctcgac ctgtatcgta aagttcagtc atccggaacc agagatgtca
      901 gtcaggaaat tcgtgagact gaagctttac tgtagaaata aaaacaaggg ccggggaatt
      961 tcattctgcc catattctct ttattgctgt gcgaaatcag actattacgg ctcaaacggg
     1021 cgtctctgga actggtcata accacatttc gggcatagcg tcagcacttc cggtgtgtag
     1081 atcgggagat ggaagtgaca tttctcgcag accagatttc ccagcccgac cacttctccg
     1141 ctgtgataaa ccccatgatg attgaggtcc tggaaaattt cgcgccattc aagctgcgtt
     1201 ttatcggtga tgtctgccag ctcctgccac aagctttctt taatcacccg cataaagacg
     1261 ctgtcagatt cttctttcag gctctcttca tagctcatgg cgaactcttc caggtcacgt
     1321 ctgacagctc gcgtcagctc atcgatctcg gttcgcgtta actcccctgt ttttattacg
     1381 cgctcgcgcg cctgttccac cagtgcgtcg atatcacgtt cgccattgcg caggcgttcg
     1441 ttcagtgacg cgaccagttc acggtaatat tgagcaacct tgttcatcgt ttcatctccc
     1501 gggtcgttaa ctgactataa taatagacgt tctttgttcc ttgctttgct aatacgccca
     1561 caaactctgt aaattaatgt acgaataatc cgggcatcat ttcaacgtcg agtgcgcgaa
     1621 aggctgtttt gacgcggacg tttgggctat gctatgcgga tctgaaaaac cacctcaacg
     1681 ctacatttgt agccgtattg aaaacaggac cactggctgc catgcaagag caataccgcc
     1741 cggaagagat agaatccaaa gtacagcttc attgggatga gaagcgcaca tttgaagtaa
     1801 ccgaagacga gagcaaagag aagtattact gcctgtctat gcttccctat ccttctggtc
     1861 gactacacat gggccacgta cgtaactaca ccatcggtga cgtgatcgcc cgttaccagc
     1921 gcatgctggg caaaaacgtt ctgcagccga tcggctggga cgcgtttggt ctgcctgcgg
     1981 aaggcgcggc ggtgaaaaac aacaccgctc cggcaccgtg gacgtacgac aacatcgcgt
     2041 atatgaaaaa ccagctcaaa atgctgggct ttggttatga ctggagccgc gagctggcaa
     2101 cctgtacgcc ggaatactac cgttgggaac agaaattctt caccgagctg tataaaaaag
     2161 gcctggtata taaaaagact tctgcggtca actggtgccc gaacgaccag accgtactgg
     2221 cgaacgaaca agttatcgac ggctgctgct ggcgctgcga taccaaagtt gaacgtaaag
     2281 agatcccgca gtggtttatc aaaatcactg cttacgctga cgagctgctc aacgatctgg
     2341 ataaactgga tcactggcca gacaccgtta aaaccatgca gcgtaactgg atcggtcgtt
     2401 ccgaaggcgt ggagatcacc ttcaacgtta acgactatga caacacgctg accgtttaca
     2461 ctacccgccc ggacaccttt atgggttgta cctacctggc ggtagctgcg ggtcatccgc
     2521 tggcgcagaa agcggcggaa aataatcctg aactggcggc ctttattgac gaatgccgta
     2581 acaccaaagt tgccgaagct gaaatggcga cgatggagaa aaaaggcgtc gatactggct
     2641 ttaaagctgt tcacccatta acgggcgaag aaattcccgt ttgggcagca aacttcgtat
     2701 tgatggagta cggcacgggc gcagttatgg cggttccggg tcacgaccag cgcgactacg
     2761 agtttgcctc taaatacggc ctgaacatca aaccggttat cctggcggct gacggctctg
     2821 agccagatct ttctcagcaa gccctgactg aaaaaggcgt gctgttcaac tctggcgagt
     2881 tcaacggtct tgaccatgaa gcggccttca acgccatcgc cgataaactg actgcgatgg
     2941 gcgttggcga gcgtaaagtg aactaccgcc tgcgcgactg gggtgtttct cgccagcgtt
     3001 actggggcgc gccgattccg atggtgacgc tggaagacgg taccgtaatg ccaaccccgg
     3061 acgaccagct gccggtgatc ctgccggaag atgtggtgat ggacggcatt accagcccga
     3121 ttaaagcaga tccggagtgg gcaaaaacta ccgttaacgg tatgccagcg ctgcgtgaaa
     3181 ccgacacttt cgacaccttt atggagtcct cctggtacta tgcgcgctac acttgcccag
     3241 agtacaaaga aggtatgctg gattccgaag cggctaacta ctggctgccg gtggatatct
     3301 acattggtgg tatcgaacac gccattatgc acctgctcta cttccgcttc ttccacaaac
     3361 tgatgcgtga tgcaggcatg gtgaactctg acgaaccagc gaaacagttg ctgtgtcagg
     3421 gtatggtgct ggcagatgcc ttctactatg ttggcgaaaa cggcgaacgt aactgggttt
     3481 ccccggttga tgctatcgtt gaacgtgacg agaaaggccg tatcgtgaaa gcgaaagatg
     3541 cggcaggcca tgaactggtt tataccggca tgagcaaaat gtccaagtcg aagaacaacg
     3601 gtatcgaccc gcaggtgatg gttgaacgtt acggcgcgga caccgttcgt ctgtttatga
     3661 tgtttgcttc tccggctgat atgactctcg aatggcagga atccggtgtg gaaggggcta
     3721 accgcttcct gaaacgtgtc tggaaactgg tgtacgagca cacagcaaaa ggtgatgttg
     3781 cggcactgaa cgttgatgcg ctgactgaag atcagaaagc gctgcgtcgc gatgtgcata
     3841 aaactatcgc taaagtgacc gatgatatcg gccgtcgtca gaccttcaac accgcaattg
     3901 cggcgattat ggagctgatg aacaaactgg cgaaagcacc gaccgatggc gagcaggatc
     3961 gcgctctgat gcaggaagca ctgcaggccg ttgtccgtat gctcaacccg ttcaccccgc
     4021 acatctgctt cacgctgtgg caggaactca aaggcgaagg cgatatcgac aacgcgccgt
     4081 ggccggttgc tgacgaaaaa gcgatggtgg aagactccac gctggtcgtg gtgcaggtta
     4141 acggtaaagt ccgtgccaaa atcaccgtcc cggtggacgc aacggaagaa caggttcgcg
     4201 aacgtgctgg ccaggaacat ctggtagcaa aatatcttga tggcgttact gtacgtaaag
     4261 tgatttacgt accaggtaaa ctcctcaatc tggtcgttgg ctaagcgcgg gaggaagcgt
     4321 gcgatatctg gcaacactgt tgttatctct ggcggtgtta atcaccgccg ggtgtggctg
     4381 gcatctgcgt gacaccacgc aggttccttc cactatgaag gtcatgatcc tggactcagg
     4441 cgatccgaac gggccattaa gccgtgcggt gcgtaaccag ttacgtctga atggtgtcga
     4501 gttgcttgat aaagaaacca cgcgtaagga cgttccatcc ctgcgtttgg gtaaagtgag
     4561 catcgcgaaa gataccgcat cggtattccg taacggtcaa acagcagagt atcagatgat
     4621 catgacggtg aatgcgaccg tgttgatccc cggccgtgat atctacccga ttagcgccaa
     4681 agtcttccgt tcattcttcg ataacccgca aatggcgtta gcgaaagata acgaacaaga
     4741 catgatcgta aaagagatgt acgaccgtgc tgccgaacag ctgattcgta agctgccaag
     4801 catccgtgct gcggatattc gttccgacga agaacagacg tcgaccacaa cggatactcc
     4861 ggcaacgcct gcacgcgtct ccaccatgct gggtaactga tgattcggtt gtacccggaa
     4921 caactccgcg cgcagctcaa tgaagggctg cgcgcggcgt atcttttact tggtaacgat
     4981 cctctgttat tgcaggaaag ccaggacgct gttcgccagg tagctgcggc acaaggattc
     5041 gaagaacacc acactttttc cattgatccc aacactgact ggaatgcgat cttttcgtta
     5101 tgccaggcta tgagtctgtt tgccagtcga caaacgctat tgctgttgtt accagaaaac
     5161 ggaccgaatg cggcgatcaa tgaacaactt ctcacactca ccggacttct gcatgacgac
     5221 ctgctattga tcgtccgcgg taataaatta agcaaagcgc aagaaaatgc cgcctggttt
     5281 actgctcttg cgaatcgcag cgtgcaggtg acctgtcaga caccggagca ggctcagctt
     5341 ccccgctggg ttgctgtgcg cgcaaaacag ctcaacttag aactggatga cgcggcgaat
     5401 caggtgctct gctactgtta tgaaggtaac ctgctggcgc tggctcaggc actggagcgt
     5461 ttatcgctgc tctggccaga cggcaaactg acattaccgc gcgttgaaca ggcggtgaat
     5521 gatgccgcgc atttcacccc ttttcattgg gttgatgctt tgttgatggg aaaaagtaag
     5581 cgcgcgttgc atattcttca gcaactgcgt ctggaaggca gcgagccggt tattttgttg
     5641 cgcacattac aacgtgaact gttgttactg gttaacctga aacgccagtc tgcccatacg
     5701 ccactgcgtg cgttgtttga taagcatcgg gtatggcaga accgccgggg catgatgggc
     5761 gaggcgttaa atcgcttaag tcagccgcag ttacgtcagg ccgtgcaact cctgacacga
     5821 acggaactca ccctcaaaca agattacggt cagtcagtgt gggcagagct ggaagggtta
     5881 tctcttctgt tgtgccataa acccctggcg gacgtattta tcgacggttg atatgaaatc
     5941 tttacaggct ctgtttggcg gcacctttga tccggtgcac tatggtcatc taaaacccgt
     6001 ggaaacgctg gcgaatttga ttggtctgac gcgggtcaca atcatcccta ataatgttcc
     6061 tccgcatcgt ccccagccgg aagcgaacag cgtgcagcgt aaacacatgc ttgaactggc
     6121 gattgccgac aagccattat ttactcttga tgaacgcgag ctaaagcgca atgccccctc
     6181 ttacactgcg caaacactga aagagtggcg gcaggaacaa ggaccggacg tgccgctggc
     6241 gtttattatt ggtcaggatt cattgctgac ctttccgacc tggtacgaat acgaaacgat
     6301 actcgacaat gcacatttga tcgtctgtcg gcgtccaggt tacccacttg aaatggcgca
     6361 accgcaatac cagcaatggc tggaagatca tttgacacat aacccggaag atcttcacct
     6421 tcagcctgcc ggtaaaattt atctggctga aacgccgtgg tttaacatct cggcgaccat
     6481 catccgcgaa cgtttgcaaa acggtgaatc gtgtgaggat ttattgccgg aaccggtatt
     6541 gacttacatt aaccaacaag gcttgtatcg ctgatacctg tcgttacgga gggcatgatg
     6601 cgactgtggt taattcgtca tggcgaaaca caagcgaatg tcgatggtct gtacagcggt
     6661 catgcgccca cacccctgac cgcacgcggt attgagcaag cgcaaaatct gcatacactg
     6721 ctagatgatg tttcctttga cttggtttta tgcagtgaac tggaacgggc acagcatacc
     6781 gcgcgactgg ttctcagtga ccgccagctc cccgttcata tcatccctga actcaacgaa
     6841 atgttttttg gcgactggga gatgcgacat catcgcgacc tcatgcaaga agatgccgaa
     6901 aactatagcg cgtggtgcaa tgactggcag catgctatcc ccacgaacgg tgaaggattt
     6961 caggcatttt cgcaacgtgt ggaacgcttt atcgcaaggc ttagtgaata tcagcactat
     7021 cagaatattt taatcgtcag ccatcagggc gtactgagtc tgttaattgc tcgtttaatt
     7081 ggcatgcctg ccgagtctat gtggcatttt cgcgttgacc aggggtgttg gagcgccatt
     7141 gatatcaacc aaaaattcgc gacgctacgc gtcctcaata gccgtgccat cggggtcgag
     7201 aacgcatgac ttttctgttt ttttacgggt aagccgcaac gaccattgac agccccgggc
     7261 aggctgatat tctccgcagc cagacttttt ccgccagaca cgactttgta gaaattgttt
     7321 tacaaaaatg gcgatgcaat ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc
     7381 gattcggcga caattgtccc gaaatcgcct ctggttcagg tatactgaca gaccattttt
     7441 atctatttga ttcacccagg gggaaaactt gcagggtaaa gcactccagg attttgttat
     7501 cgacaaaatt gatgacctca aaggtcagga catcatcgcc ttagacgttc aaggcaaatc
     7561 cagcatcacc gactgcatga tcatctgtac gggtacgtcc agccgtcatg ttatgtccat
     7621 tgctgaccac gttgtgcagg agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg
     7681 tgaaaacagc gccgactgga ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca
     7741 ggaagagagc cgtcgcctgt atgaactgga aaaactctgg agttaatgcg tgaagctgca
     7801 acttgtcgcc gtgggaacga aaatgccgga ctgggtacaa accggtttta ccgagtacct
     7861 gcgtcgtttt ccgaaagata tgcccttcga gctgattgaa attccggccg gcaaacgcgg
     7921 caagaatgcg gacatcaagc gcatactcga caaagagggt gagcagatgt tggcggccgc
     7981 aggcaaaaac cgcattgtca ccctcgatat tccaggcaag ccctgggata cgccgcagtt
     8041 agccgctgag ctggaacgct ggaagctgga tggtcgcgac gtaagtctac tgattggcgg
     8101 gcctgaaggg ttgtcgcctg cctgtaaagc ggcggctgag cagagctggt cgctgtcggc
     8161 gcttaccctc ccccatccgc tggttcgcgt gctggtcgca gagagtctgt accgggcgtg
     8221 gagcatcacc accaaccatc cttatcaccg tgagtgataa gggagctttg agtagaaaac
     8281 gcagcggatg aaactacaga actcttttcg cgactatacg gcagagtccg cgctgtttgt
     8341 gcgccgggcg ctggtcgcct ttttggggat tttgctgctg accggcgtgc ttatcgccaa
     8401 cctgtataat ctgcaaattg ttcgctttac cgactaccag acccgctcta atgaaaaccg
     8461 cattaagctg gtgcctatcg cgcccagccg cggcattatc tacgaccgta acggtatccc
     8521 tctggccctc aaccgcacta tctaccagat agaaatgatg ccggagaaag tcgataacgt
     8581 gcagcaaacg ctggacgctt tgcgcagcgt ggtagatctg accgatgacg atattgctgc
     8641 attccgaaaa gagcgcgcac gttcacaccg tttcacctct attccggtga aaactaacct
     8701 aaccgaagta caagtagctc gctttgccgt caatcagtac cgttttccgg gtgtcgaagt
     8761 taaaggctat aaacgtcgtt actatcctta cggttcggcg ttgacccacg tcatcggcta
     8821 tgtgtcgaaa atcaacgata aagacgtcga acgcctgaat aatgacggca aactggccaa
     8881 ctatgcggca acgcatgata ttggtaagct gggcattgag cgttactatg aagacgtgct
     8941 gcacggtcag accggttatg aagaggttga agttaacaac cgtgggcgtg ttatccgcca
     9001 gttaaaagaa gtaccaccgc aagccggaca cgatatttac ctgacgctgg atctcaaact
     9061 ccagcaatat attgaaacgc tgctggcggg tagccgcgca gctgtggttg ttaccgaacc
     9121 gcgtacaggt ggggtgctgg cgctggtttc cacgcctagt tatgacccaa acctgtttgt
     9181 tgacggtatc tccagcaaag attattccgc cttgctgaac gacccgaata caccgctggt
     9241 gaaccgcgcc acacaggggg tttatcctcc cgcgtctaca gttaaaccct atgtggcggt
     9301 ttcggcatta agcgccgggg tgatcacgcg caatacgacg ctgtttgacc caggctggtg
     9361 gcaactacca gggtcggaaa aacgttatcg tgactggaaa aaatggggcc acgggcgtct
     9421 gaatgtcaca agatcgctgg aagaatctgc ggataccttc ttctatcagg tggcctacga
     9481 tatggggatc gatcgcctct ccgaatggat gggtaaattc ggttatggtc attacaccgg
     9541 tatcgacctg gcggaagaac gttccggcaa catgcctacc cgcgaatgga aacagaaacg
     9601 ctttaaaaaa ccgtggtatc agggtgacac cattccggtt ggtatcggcc agggttactg
     9661 gacagcgacc ccaatccaga tgagtaaggc actgatgatc ctgattaatg acggtatcgt
     9721 gaaggttccg catttgctga tgagcaccgc cgaagacggc aaacaggtgc catgggtaca
     9781 accgcatgaa ccgcccgtcg gcgatattca ttccggttac tgggagctgg cgaaagacgg
     9841 tatgtacggt gttgctaacc gccctaatgg tacggcgaat aaatactttg ccagcgcacc
     9901 gtacaaaatt gcggcgaaat ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac
     9961 ctataatgcg cacaaaattg ccgagcgttt acgtgaccac aaactgatga ccgcctttgc
    10021 gccatacaac aatccgcaag tggctgtcgc catgattctg gagaacggcg gcgcgggtcc
    10081 ggcggttggt acgctgatgc gccagatcct cgaccacatt atgctgggtg ataacaacac
    10141 cgatctgcct gcggaaaatc cagcggttgc cgcagcggag gaccattaat catgacggat
    10201 aatccgaata aaaaaacatt ctgggataaa gtccatctcg atcccacaat gctgctgatt
    10261 ttactggcat tgctggttta cagcgccctg gttatctgga gcgccagcgg tcaggatatt
    10321 ggcatgatgg agcgtaaaat cggccaaatc gcgatgggtc tggtcatcat ggtggtgatg
    10381 gcgcaaattc ctccacgcgt ttatgaaggc tgggccccct atctctatat catctgtatt
    10441 attttgctgg tggcggtaga tgctttcgga gccatctcta aaggcgctca acgctggctg
    10501 gacctcggta ttgttcgttt tcagccgtcg gaaattgcca aaatagccgt accactgatg
    10561 gttgcgcgct ttatcaaccg cgacgtttgc ccgccatcgt tgaagaatac tggcatcgcg
    10621 ctggtgctga tatttatgcc cacgctgctg gtggctgcac agcctgacct gggaacatca
    10681 atcctcgttg cgctttccgg tctgtttgta ctgttcctct ctggccttag ctggcgtctg
    10741 attggcgtcg cagtagtgct ggtagcggcg ttcattccga ttctgtggtt cttcctgatg
    10801 catgattacc agcgccagcg cgtaatgatg ctcctggacc cggaatcaga cccactcggc
    10861 gcgggctatc acattattca gtctaaaatt gctattggct ccggcggatt acgcggcaaa
    10921 ggctggctgc acggcactca gtcacagctt gaatttctcc ccgaacgcca tactgacttt
    10981 atcttcgcgg tactggcgga agagctggga ttagtgggca ttctgattct gctcgctctc
    11041 tacattctgc tgatcatgcg cgggctgtgg atagccgcca gagcgcaaac cacctttggt
    11101 cgcgtcatgg ctggtggctt aatgctgata ttattcgttt atgtcttcgt aaatattggt
    11161 atggtaagcg gtattctgcc ggttgtaggg gttccgctcc cactggtcag ttatggagga
    11221 tcggcgctaa ttgtgctgat ggctgggttc gggattgtaa tgtcaatcca cacccacagg
    11281 aaaatgttgt cgaaaagcgt gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg
    11341 catcgcggca ggaatgctcg cggcatgtac aagcgatgat ggtcagcaac agacggtaag
    11401 tgtaccgcag cctgcggtat gtaacggccc catagttgaa attagcgggg cggacccgcg
    11461 tttcgaacca ctgaacgcga cggcaaatca ggattaccag cgcgacggta aaagctacaa
    11521 aatcgtgcag gatccgtctc gatttagcca ggcgggactg gcggcaatct atgatgccga
    11581 accaggcagt aacctgacgg cctctggcga agctttcgat ccgacaaagc tgacggcggc
    11641 ccatccaacg cttccgatcc ccagctacgc cagaatcact aacctggcta acgggcgaat
    11701 gatcgtggtg cgcattaatg atcgcggccc ttacggcaac gaccgcgtta tttcgctttc
    11761 tcgcgcggca gctgaccgtc ttaacacgtc aaacaacacc aaagttcgta tcgatccgat
    11821 tattgtcgcc caggatggtt cgctttctgg tcctggtatg gcttgtacca cagtcgccaa
    11881 acagacttac gccctgcctg cacctcccga tttaagcggt ggcgcgggaa caagttcagt
    11941 gtctggcccg cagggtgaca ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc
    12001 gaccggcgcg ccggtaacca gtagcggttt cctcggcgct ccaacgacct tagcgcctgg
    12061 tgtactggaa ggcagcgaac cgacgcctgc gccacagccc gttgttacag cttcgtcgac
    12121 aacgcctgca acctcgcctg caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg
    12181 caactttatg gtgcaagtcg gggccgtaag cgatcaggct cgtgcgcaac agtaccaaca
    12241 gcaactggga cagaagttcg gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg
    12301 gatccagctt ggcccattcg ccagcaaagc cgaagccagt accttgcagc aacgtttgca
    12361 aaccgaagcc caattacagt catttattac taccgcgcag tagcgtaaag caggcatctg
    12421 atgtgtcaat ttctgtaaat gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg
    12481 tagtgcattt gctatagtag ggcacttttt taattccatc acggatgtcg tagttcagac
    12541 catgaatacc attttttccg ctcgtatcat gaagcgcctg gcgctcacca cggctctttg
    12601 cacagccttt atctctgctg cacatgccga tgacctgaat atcaaaacta tgatcccggg
    12661 tgtaccgcag atcgatgcgg agtcctacat cctgattgac tataactccg gcaaagtgct
    12721 cgccgaacag aacgcagatg tccgccgcga tcctgccagc ctgaccaaaa tgatgaccag
    12781 ttacgttatc ggccaggcaa tgaaagccgg taaatttaaa gaaactgatt tagtcactat
    12841 cggcaacgac gcatgggcca ccggtaaccc ggtgtttaaa ggttcttcgc tgatgttcct
    12901 caaaccgggc atgcaggttc cggtttctca gctgatccgc ggtattaacc tgcaatcggg
    12961 taacgatgct tgtgtcgcca tggccgattt tgccgctggt agccaggacg cttttgttgg
    13021 cttgatgaac agctacgtta acgcactggg cctgaaaaat acccacttcc agacggtaca
    13081 tggtctggat gctgatggtc agtacagctc cgcgcgagat atggcgctga tcggccaggc
    13141 attgatccgt gacgtaccga atgaatactc gatctataaa gaaaaagaat ttacgtttaa
    13201 cggtattcgc cagctgaacc gtaacggcct gttatgggat aacagcctga atgtcgacgg
    13261 catcaaaacc ggacacactg acaaagcagg ttacaatctt gttgcttctg cgactgaagg
    13321 ccagatgcgc ttgatttctg cggtgatggg tggacgtact tttaaaggcc gtgaagccga
    13381 aagtaaaaaa ctgctaacct ggggcttccg tttcttcgaa accgttaacc cactgaaagt
    13441 aggtaaagag ttcgcctctg aaccggtttg gtttagtgat tctgatcgcg cttcgttagg
    13501 ggttgataaa gacgtgtacc tgaccattcc gcgtggtcgc atgaaagatc tgaaagccag
    13561 ctatgtgctg aacagcagtg aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac
    13621 tatcaacttc cagcttgatg gcaaaacgat cgagcaacgc ccgctggttg tgttgcaaga
    13681 aatcccggaa ggtaacttct tcggcaaaat cattgattac attaaattaa tgttccatca
    13741 ctggtttggt taaaaattga acacttgaaa gtgtaatttc cgtccccata tactaagcat
    13801 cagtaaaaaa actcccgcct tctggcggga gttgctattt aattacgtta cgccggagct
    13861 gacatgaaaa ccaaacttaa cgaactgctt gaattcccta ctccttttac ttacaaagtt
    13921 atggggcagg cgttacctga gctggttgat caggtggttg aagtggtaca gcgccatgcg
    13981 ccaggtgact acaccccaac ggtaaaacca agcagcaaag gcaactacca ctcggtatct
    14041 atcactatca acgccactca tatcgagcag gttgaaacac tgtatgaaga actgggcaaa
    14101 atcgatattg tccgcatggt tctgtaactc gcttctccgt tacccggctc ctggtcgggt
    14161 aactcccctc tcctcgctgt gatatacttt tcccccactt ttactcattc tccacggaga
    14221 tgccgttttg tatcaggata aaattcttgt ccgccagctc ggtcttcagc cttacgagcc
    14281 aatctcccgg gctatgcatg aattcaccga tacccgcgat aatagtaccc ttgatgaaat
    14341 ctggctggtc gagcactatc cggtattcac ccaaggtcag gcaggaaaag cggagcacat
    14401 tttaatgccg ggtgatattc cggtgatcca gagcgatcgc ggtgggcagg tgacttatca
    14461 cgggccggga caacaggtga tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt
    14521 gcgtgaactg gtgaccttgc ttgagcaaac agtggtgaat accctggctg aactgggtat
    14581 agaagcgcat cctcgggctg acgcgcctgg tgtctatgtc ggggaaaaga aaatttgctc
    14641 actgggttta cgaattcgac gcggttgttc attccacggt ctggcattaa acgtcaatat
    14701 ggatctttca ccatttttac gtattaatcc ttgtgggtat gccggaatgg aaatggctaa
    14761 aatatcacaa tggaaacccg aagcgacgac taataatatt gctccacgtt tactggaaaa
    14821 tattttagcg ctactaaaca atccggactt cgaatatatt accgcttaat tccatacatc
    14881 aatggcccaa tttacactgg gtcattactc ataatacctg ctaatgcttt acatctcctt
    14941 cgcgtttgta tattttcttt atcacttgca gaaatgcggt gccacgcctt gctccgtaac
    15001 tcaccccatt gattaaccta ttgaaatagt tgattatggt gagcaaaagc gttccgttcc
    15061 aattagacat aagattattg taatttttta aatattattt ttccatgaat aaaattggag
    15121 taagtgcgtg gatagtaata atcaaattga accctgttta agcagaaagt cgtcagaagg
    15181 taaaccacaa atatttacaa ccttacgaaa tattgatctt aaccttctga ctatttttga
    15241 agctgtatat gtacataaag ggatcgttaa tgcagcgaaa gtgcttaatc tgaccccctc
    15301 ggcaatcagt cagtctattc agaaactgcg cgttatattc cctgacccat tgtttattcg
    15361 caaaggccag ggtgtcactc ctaccgcatt tgcgatgcat ctacatgagt atatcagtca
    15421 gggccttgag tccattcttg gcgcgctgga tatcgaagga agctatgata agcaacgaac
    15481 gataactatt gcgaccactc cctcggtcgg agccctggtc cttcctgtca tctatcgggc
    15541 gattaaaact cactatccgc agcttttact gcgcaacccg cccatcagcg acgcggaaaa
    15601 ccaactcagt cagtttcaaa ccgatctcat catcgataac atgttttgca ccaatcgtac
    15661 ggtgcaacat catgttctgt tcaccgacaa tatggtgtta atttgccgca agggaaatcc
    15721 actactctct ttagaagatg acagagagac tatcgacaac gctgcgcatg tacttctgtt
    15781 accggaaggg caaaatttca gcggtctgcg gcagagagtt caagagatgt ttccggaccg
    15841 gcaaatcaat ttcaccagct acaacatttt gacaatcgct gcactggttg ccaacagtga
    15901 catgttagcg attattccca gccgttttta taacctgttt agtcgttgct ggccgctgga
    15961 aaaactgcct tttccgtcct taaatgagga gcaaatagat ttctctatcc actacaacaa
    16021 attcatcctg cgtgatccca tcctgcacgg agtaatagat gttatccgta atgcattttg
    16081 aaaaagtatt gttacagcca gatcacagcg cagaacgata aaatggcaca atccaacaac
    16141 aattttacat tttcgcgacc gctttggctg ctttcaggtc cgtttcaatg atatactgcc
    16201 agtcgttaat tcaaaaatag ttgataatta caacaatcta ttgaattgaa acgctttcct
    16261 tcgtaattcg caactggaac acgtacgcta tgagtaaacc cattgtgatg gaacgcggtg
    16321 ttaaataccg cgatgccgat aagatggccc ttatcccggt taaaaacgtg gcaacagagc
    16381 gcgaagccct gctgcgcaag ccggaatgga tgaaaatcaa gcttccagcg gactctacac
    16441 gtatccaggg catcaaagcc gcaatgcgca aaaatggcct gcattctgtc tgcgaggaag
    16501 cctcctgccc taacctggcg gaatgcttca accacggcac agcaacgttt atgatcctcg
    16561 gcgctatttg tacccgccgt tgtccgttct gtgatgttgc ccacggtcgc ccggtagctc
    16621 ctgatgccaa tgaaccagtg aaactggcgc agaccattgc cgatatggcg ctgcgttatg
    16681 tggttatcac ctccgttgac cgtgatgacc tgcgcgatgg cggtgcccaa cactttgcgg
    16741 attgtattac tgccattcgg gaaaaaagcc cgcaaatcaa aattgaaact ctggtgccgg
    16801 atttccgcgg tcgaatggat cgtgctctgg atattctgac tgcaacgccc ccagatgtgt
    16861 tcaaccataa cctggaaaac gtaccgcgta tttaccggca ggtacggcct ggcgcagatt
    16921 acaactggtc gctgaagctg ctggaacgct ttaaagaagc gcatccggaa atcccgacca
    16981 agtctggtct gatggtgggt ctgggtgaaa ccaatgaaga aattattgag gtaatgcgcg
    17041 acctgcgccg tcatggtgtg acgatgttaa cgctggggca atatttgcag ccaagccgcc
    17101 atcacctgcc ggttcaacgt tacgttagcc cggatgagtt cgacgaaatg aaagccgaag
    17161 cgctggcgat gggctttacc catgctgcat gcggtccgtt tgtccgctct tcttaccacg
    17221 ccgatttgca ggcgaaagga atggaagtta agtaatcctg taataattac ttacatctgt
    17281 ctgataaaaa acccgctttt tgaagcgggt tttttgtatc gaacaagata ttgagggagc
    17341 gtcctgctcg ccacgtcact ctttatgaga gagcttttca gcctgaagat caacgtctgc
    17401 gccttttttc gccgcagcat cgtcatcatt catcgccttc ttgaaccctt taatggccgc
    17461 tccaaggtct ccgcccagcg tacgtaactt cttagtccca aacagcagaa cgaccagcgc
    17521 cgcaactacc agcagtttgg taatactaat ctcacccata gataccttct tgacatataa
    17581 ccggcaacag aacgcgcctt aaaaaccaga gaatattaac ggtcattcga cgcgcgcaca
    17641 caatagcaat ctgtaacaag gtgaatcaag cgccgagtaa aaaaacatca taataattgc
    17701 ggcggcgcaa agcgacggtt gtttaagatg ggcagttgcg cgcgcacctg acgcacacgt
    17761 tcgggcgtca cttccgccat aatgagtgca ggcatttctg acgctgccgc aatggtgacg
    17821 ccaaagggat ctataatccg gctttgcccg atatttttgt tcccgcactc ccccgccgcc
    17881 accatataac aggtggtatc cagcgcacga gcggcaagca acgttgacca gtgatgctct
    17941 ttgagcggcc cacgaaccca ggcggcagga agtaccagga tttcagctcc ctgtaaagcc
    18001 tgtgccagcg ccagctctgg aaagcgtaag tcataacagg tcatcagacc gaccttcatc
    18061 ccttccacct ccagtaacgg agctatttca ttaccagcat caacatggcg tgattcctga
    18121 atggcaaatg catcatagag atgcagtttg gcataacggg cgacgatgtt tcctgcctga
    18181 agtgccacca gcatattcca tgcgcgcccc ggcgttgaag gaagatgaat cgtcagaatt
    18241 gtcgtcatca tgttacgttt actttctcgc cgtaaacgtc cgaggaattc gccttccagc
    18301 agctgtgctg atttaaccga tagatctgca tcatgatcat cgcgcgccag caatgcttcc
    18361 ggcaggacaa acagcgatac gtcgttttcc gccgcctgcg ccatcaacga ggcacaaatc
    18421 tcagcgttct tttcccacac agatgtaaca gcaaactgtc ctgcagcaac cagcattgcc
    18481 acctcctgtc agagtcgtaa aattcgccaa cgacgattat tcggcgttac acttatcact
    18541 catacaaatc aaatagcagg attttgcagt gttacaactt cttttagcag tttttattgg
    18601 cggtggtacg ggaagcgtag cgagatggct gttaagtatg cgatttaacc cgctgcatca
    18661 ggcgattccg ttggggacgc tggcagcaaa cctgattggg gcattcatca taggaatggg
    18721 attcgcctgg ttcagcagga tgacgaacat tgatccagtg tggaaagtat taatcaccac
    18781 cggattttgt ggcggtctaa caaccttctc aacattttcg gcagaagtgg tgtttttgtt
    18841 acaagagggc cgctttggct gggcattact gaacgttttc gtcaaccttc tggggtcttt
    18901 tgccatgacc gcactggcat tctggctgtt ttcggcctca accgtacact aaagaccaaa
    18961 aaaaacccgc tgattaagcg ggttttgaat tcttgctgac gtatcttaca gagcgattac
    19021 gtttgcagca gaagggcctt tggcaccgtt agtgatttcg aactctacgc gctgaccttc
    19081 agcaagagtt ttaaaaccat tagtctggat tgcagagaag tgtacgaaca cgtctttgct
    19141 gccgtcttcc ggagtaatga aaccgaatcc tttggactca ttaaaccact taacgttacc
    19201 tttaatctta gacatcaaaa ttacctttac atgaaaaata gacacaaatg ctgtgtcggt
    19261 tatcagtaca ccaattgtgg tacgctttgt ccagtgcaac tttactaaaa agtgataaaa
    19321 gtcgcgttat ttttgatgtt gcttatttta gttattgatt ttaaagaagt tactaaaact
    19381 tcatttgtct caaaactgaa agcgcatcca ggcaaagtac acattgccat tgttgtaggt
    19441 acccggaatg taggtcatct gaaaagtcac tgggccataa cccacggagg ccaatggcag
    19501 tagaaccggg agagggatgt aattccagtt atcgcgtgcc gttacgccag cggtgaatcc
    19561 cagacctaaa tgaaaatttt catccgccag cggtcgccag gtactttccc atccgtatcc
    19621 ggcaatcggt tcccatttgt tccacgagtc cttaaatgcc atggcataca ggccatgcca
    19681 gtttcctttt tcatcccaac gcgacaggcc aaaaccgcca ccccacggtc gctcgttata
    19741 gcgatcggtt ttttctttgt cgtaagcgaa acgtgcatgc caggtgatgg caggaatata
    19801 taaatcataa tgttcaggct gttgccaggt ttgtgcaata ttttctctaa acgttgtcat
    19861 cctctcatct gcgttagcaa aaactttacc aacgctgatt aactgaataa aaacaaagga
    19921 aaagatagcg acatatttac tcacgttcat ttgtgaccat aaaacattta tcaaaaatct
    19981 actactagca tagcaaagct acaattaaca t
//
LOCUS       DAZXUK010000048        19418 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZXUK010000048 DAZXUK010000000
VERSION     DAZXUK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19418
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.048"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..122
                     /locus_tag="ACH3L8_001414"
     CDS             <1..122
                     /locus_tag="ACH3L8_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFT4084590.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            138..686
                     /gene="wcaF"
                     /locus_tag="ACH3L8_001415"
     CDS             138..686
                     /gene="wcaF"
                     /locus_tag="ACH3L8_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFT4084591.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            712..1833
                     /gene="gmd"
                     /locus_tag="ACH3L8_001416"
     CDS             712..1833
                     /gene="gmd"
                     /locus_tag="ACH3L8_001416"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFT4084592.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            1836..2804
                     /gene="fcl"
                     /locus_tag="ACH3L8_001417"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             1836..2804
                     /gene="fcl"
                     /locus_tag="ACH3L8_001417"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFT4084593.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            2804..3283
                     /gene="gmm"
                     /locus_tag="ACH3L8_001418"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             2804..3283
                     /gene="gmm"
                     /locus_tag="ACH3L8_001418"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFT4084594.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            3280..4503
                     /gene="wcaI"
                     /locus_tag="ACH3L8_001419"
     CDS             3280..4503
                     /gene="wcaI"
                     /locus_tag="ACH3L8_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFT4084595.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            4506..5942
                     /gene="cpsB"
                     /locus_tag="ACH3L8_001420"
     CDS             4506..5942
                     /gene="cpsB"
                     /locus_tag="ACH3L8_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFT4084596.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            6135..7505
                     /gene="cpsG"
                     /locus_tag="ACH3L8_001421"
                     /gene_synonym="manB"
     CDS             6135..7505
                     /gene="cpsG"
                     /locus_tag="ACH3L8_001421"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFT4084597.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACH3L8_001422"
     CDS             7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACH3L8_001422"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFT4084598.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            8956..10434
                     /gene="wzxC"
                     /locus_tag="ACH3L8_001423"
     CDS             8956..10434
                     /gene="wzxC"
                     /locus_tag="ACH3L8_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFT4084599.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            10614..11894
                     /gene="wcaK"
                     /locus_tag="ACH3L8_001424"
     CDS             10614..11894
                     /gene="wcaK"
                     /locus_tag="ACH3L8_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFT4084600.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            11891..13111
                     /gene="wcaL"
                     /locus_tag="ACH3L8_001425"
     CDS             11891..13111
                     /gene="wcaL"
                     /locus_tag="ACH3L8_001425"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFT4084601.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            13122..14516
                     /gene="wcaM"
                     /locus_tag="ACH3L8_001426"
     CDS             13122..14516
                     /gene="wcaM"
                     /locus_tag="ACH3L8_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFT4084602.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            14691..15584
                     /gene="galF"
                     /locus_tag="ACH3L8_001427"
     CDS             14691..15584
                     /gene="galF"
                     /locus_tag="ACH3L8_001427"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFT4084603.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            15957..17042
                     /gene="rfbB"
                     /locus_tag="ACH3L8_001428"
     CDS             15957..17042
                     /gene="rfbB"
                     /locus_tag="ACH3L8_001428"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFT4084604.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            17042..17941
                     /gene="rfbD"
                     /locus_tag="ACH3L8_001429"
     CDS             17042..17941
                     /gene="rfbD"
                     /locus_tag="ACH3L8_001429"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFT4084605.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            18000..18878
                     /gene="rfbA"
                     /locus_tag="ACH3L8_001430"
     CDS             18000..18878
                     /gene="rfbA"
                     /locus_tag="ACH3L8_001430"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFT4084606.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            18883..>19418
                     /gene="rfbC"
                     /locus_tag="ACH3L8_001431"
     CDS             18883..>19418
                     /gene="rfbC"
                     /locus_tag="ACH3L8_001431"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFT4084607.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDAD"
BASE COUNT         4839 a         4692 c         5288 g         4599 t
ORIGIN      
        1 gtgctgacgc caaaaaagtc caacggcaga tattacatgt acctggcttc tgggcagaat
       61 tatcctggca tttacgccaa cgtacgacag gtaagactaa agccttatat aacaaagtct
      121 gaatataagg aaaaccaatg caagatttaa gcggtttctc ggtgccgaaa gggttccggg
      181 gcggcaacgc tattaaagtg caattatggt gggcagtaca ggcaacaata tttgcctggt
      241 cgccacaagt attgtatcgc tggcgggctt ttttattacg tttattcgga gcaaaaatag
      301 gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag
      361 gtgattacgc gtgggttggc gatgacgtca atttatatac cctcggtgaa ataaccattg
      421 gcgcacattc ggtgatatcg caaaaaagtt atttatgcac cggtagccac gaccatgcaa
      481 gtcaacattt caccattaac gccacgccta ttgtgattgg cgagaaatgc tggctggcaa
      541 ccgatgtctt tgttgcccca ggcgtcacaa tcggcgacgg caccgtcgtg ggtgcacgaa
      601 gcagtgtttt taaatcgctt ccggcaaatg tggtttgccg ggggaatccc gcagtggtga
      661 tacgcgaacg cgttgaaact gaataaattc aaaaatacag aggaataaga catgtcaaaa
      721 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg
      781 gaaaaaggtt acgaggtgca tggtattaag cgtcgcgcat cgtcattcaa caccgagcgc
      841 gtggatcaca tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc
      901 gacctgagtg atacctctaa cctgacgcgc attttgcgtg aagtacagcc ggatgaagtg
      961 tacaacctag gcgcaatgag ccacgttgcg gtctcttttg agtcaccaga atataccgct
     1021 gacgtcgacg cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa
     1081 aagaaaactc gtttctatca ggcttccacc tctgaactgt atggtctggt gcaggaaatt
     1141 ccgcagaaag aaaccacgcc gttctacccg cgatctccgt atgcggtcgc caaactgtac
     1201 gcctactgga tcaccgttaa ctaccgcgaa tcctacggca tgtacgcttg taacggaatt
     1261 ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg ttacccgcaa aatcacccgc
     1321 gcaatcgcca acatcgccca ggggctggag tcgtgcctgt acctcggcaa tatggattcc
     1381 ctgcgtgact ggggccacgc caaagactac gtaaaaatgc agtggatgat gctgcaacag
     1441 gaacagccgg aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagtttgtg
     1501 gaaatggcgg cagcacagct gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag
     1561 aagggcattg tggtttccgt caccgggcat gacgcgccgg gcgttaaacc gggtgatgtg
     1621 attatcgccg ttgatccgcg ttacttccgt ccggcagaag ttgaaacgct gctcggcgac
     1681 ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa tcaccctcag agagatggtg
     1741 tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac
     1801 ggctacgacg tggcgatcgc gctggagtca taagcatgag taaacaacgc atttttatcg
     1861 ctggccattg tgggatggtc ggttctgcca tcaagcggca gctcgaacag cgcggtgatg
     1921 tggagctggt attacgtacc cgcgatgaac tgaacctgct ggacagccgc gccgtgcatg
     1981 atttctttgc cagcgaacgc attgaccagg tctatctggc ggcggcgaaa gtgggcggca
     2041 ttgttgccaa caacacctat ccggcggatt tcatctacca gaacatgatg attgagagca
     2101 acatcattca cgccgcgcat cagaacgacg tgaacaaact gatgtttctc ggatcgtcct
     2161 gcatctaccc gaaactggca aaacagccga tggcagaaag cgagttattg cagggcacgc
     2221 tggagccgac caacgagcct tacgccattg ccaaaatcgc cgggatcaaa ctgtgcgaat
     2281 cttacaaccg ccagtatgga cgcgattacc gcactgtcat gccgaccaac ctgtacgggc
     2341 cgcatgacaa cttccacccg agtaactccc atgtgatacc agcattgctg cgtcgcttcc
     2401 acgaggcgac ggcacagaat gcgccggacg tggtggtatg gggcagcggt acaccgatgc
     2461 gtgaattcct gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc
     2521 atgaagtctg gctggagaat acccaaccga tgctgtcgca cattaacgtc ggcacgggcg
     2581 ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc
     2641 gggtggtttt tgatgccagc aaaccggatg gtacgccgcg caaactgctg gatgtgacgc
     2701 gcctgcatca gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt
     2761 accagtggtt ccttgagaat caagaccgct ttcgggggga atgatgtttt tacgtcagga
     2821 agactttgcc actgtagtgc gctccacacc gcttgtctct ctcgacttta ttgtcgagaa
     2881 cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt
     2941 tgtgccggga gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac
     3001 gatggcggaa ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca
     3061 gcatttttat gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg
     3121 ttttcgcttc agagtagcgg aagaagagct gttactgccg gatgagcagc atgacgatta
     3181 ccgctggctg acgccggacg cgctgctcgc cagtaacgat gttcacgcta acagccgagc
     3241 ctattttctc gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc
     3301 attaactact cgccggagtt aaccggcatc ggcaaataca ccggcgagat ggtggaatgg
     3361 ttggcggcac aaggtcatga ggtgcgggtc attaccgcac cgccttacta cccgcaatgg
     3421 caggtgggtg agaactattc cgcctggcgc tacaaacgag aagaaggggc cgccacggtg
     3481 tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc tgaaacgctt gttgcatctc
     3541 ggcagttttg ccgtcagcag tttctttcca ctgatggcgc aacgtcgctg gaagccggat
     3601 cgcattattg gcgtagtgcc aacgctgttt tgcacgccgg gaatgcgcct gctggcgaag
     3661 ctctctggtg cgcgtaccgt gctgcatatt caggattacg aagtggacgc catgctgggg
     3721 ctgggccttg ccggaaaagg caaaggcggc aaagtggcac agctggcaac ggcgttcgaa
     3781 cgtagcggac tgcataacgt cgataacgtt tccacgattt cgcgttcgat gatgaataaa
     3841 gccatcgaaa aaggcgtggc ggcggataac gtcatcttct tccccaactg gtcggaaatt
     3901 gcccgttttc agcatgttgc agacgccgat gttgatgccc ttcgtaacca gcttggcctg
     3961 ccggataaca aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa
     4021 aacgttattg aagctgccga tctcctgcgc gatgaaccgc tgatttttgc cattgtcggg
     4081 cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc agcgtggact gcgcaacatg
     4141 caatttttcc cgctgcaatc gtatgacgct ttacctgcac tgctgaagat gggcgattgc
     4201 catctggtgg tgcaaaaacg cggcgcggca gatgccgtat tgccgtcgaa actgaccaat
     4261 attctggcgg taggcggtaa cgcggtgatt accgcagagg cccacacaga actggggcag
     4321 ctttgcgaaa cctttccggg tattgcggtt tgcgtagaac cggaatcggt cgaggcgctg
     4381 gtggcgggga ttcgtcaggc gctcctgctg cccaaacata acacggtggc acgtgaatat
     4441 gccgaacgca cgctcgataa agagaacgtg ttacgtcaat ttataaatga tattcgggga
     4501 taattatggc gcagtcgaaa ctctatccag ttgtgatggc aggtggctcc ggtagccgtt
     4561 tatggccgct ttcccgcgta ctttacccca agcagttttt atgcctgaaa ggcgatctca
     4621 ccatgctgca aaccaccatc tgccgcctga acggcgtgga gtgcgaaagc ccggtggtga
     4681 tttgcaatga gcagcaccgc tttattgtcg cggaacagct gcgtcaactg aacaaactca
     4741 ccgagaacat tattctcgaa ccggcagggc gtaacactgc acctgccatt gcactggcgg
     4801 cgctggcggc aaaacgtcat agcccggaga gcgacccgtt aatgctggta ttggcggcgg
     4861 atcatgtgat tgccgatgaa gacgcgttcc gtgccgccgt gcgtaatgcc atgccttatg
     4921 ccgaagcggg caagctggtg acctttggca ttgtgccgga tctaccagaa accggttatg
     4981 gctatattcg tcgcggtgaa gtgtctgtgg gtgagcagga tacggtggcc tttgaagtgg
     5041 cgcagtttgt cgaaaaaccg aatctggaaa ccgcccaggc ctatgtagca agcggcgaat
     5101 attactggaa cagcggcatg ttcctgttcc gcgccggacg ctatctcgaa gaactgaaaa
     5161 aatatcgccc ggatatcctc gatgcctgcg aaaaagcgat gagcgccgtc gatccggatc
     5221 tcgattttat tcgtgtggat gaagaagcgt ttctcgcctg cccggaagag tcggtggatt
     5281 acgcggtcat ggaacctacg gcagatgctg ttgtggtgcc gatggatgcg ggctggagcg
     5341 atgttggttc ctggtcttca ttatgggaga tcagcgccca caccgccgag ggcaacgttt
     5401 gccacggcga tgtgattaat cacaaaactg aaaacagcta tgtgtatgcc gaatcaggcc
     5461 tggtcaccac cgtcggggtg aaagatctgg tagtggtgca gaccaaagat gcggtgctga
     5521 ttgccgaccg taacgcggta caggatgtga aaaaagtggt cgagcagatc aaagccgatg
     5581 gtcgccatga gcatcgggtg catcgcgaag tgtatcgtcc gtggggcaaa tatgactcta
     5641 tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt gaaaccgggc gagggtttgt
     5701 cggtacagat gcaccatcac cgcgcggaac actgggtagt ggtcgcggga acggcaaaag
     5761 tcaccattga tggtgatatc aaactgcttg gtgaaaacga gtccatttat attccgctgg
     5821 gggcgacgca ctgcctggaa aacccgggga aaattccgct cgatttaatt gaagtgcgct
     5881 ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt
     5941 aaacgtcgca tcaggcgctg tttgtcggat gcggcgtgaa cgccttatcc gacctacggt
     6001 tcggttttgt aggcctgata agacgcggca gcgtcgcatc aggcaatgaa tgcgaaaccg
     6061 cggtgtaaat aacgacaaaa ataaaattgg ccgtttcggt cagggccaac tattgcctga
     6121 aaaagggtaa cgacatgaaa aaattaacct gctttaaagc ctatgatatt cgcgggaaat
     6181 taggcgaaga actgaatgaa gatatcgcct ggcgcattgg tcgcgcctat ggcgaatttc
     6241 tcaaaccgaa aaccattgtg ttaggcggtg atgtccgtct caccagcgaa accttaaaac
     6301 tggcgctggc aaaaggttta caggatgcgg gcgtcgatgt gctggatatc ggtatgtccg
     6361 gcaccgaaga gatctatttc gccacgttcc atctcggcgt ggatggcggc attgaagtta
     6421 ctgccagcca taatccgatg gattacaacg gcatgaagct ggtgcgcgag ggggctcgca
     6481 cgatcagcgg tgataccgga ctgcgcgacg tccagcgtct ggctgaagcc aacgactttc
     6541 ctcccgtcga tgaaaccaaa cgcggtcgct atcagcaaat caacctgcgt gacgcttacg
     6601 ttgatcacct gttcggttat atcaatgtca aaaatctcac gccgctcaag ctggtgatca
     6661 actccgggaa tggcgcagcg ggtccggtgg tggacgccat tgaagcccgc tttaaagccc
     6721 tcggcgcgcc cgtggaatta atcaaagtac acaacacacc ggacggcaat ttccccaacg
     6781 gtattcctaa cccgctgctg ccggaatgtc gcgacgacac ccgcaatgcg gtcatcaaac
     6841 acggcgcgga tatgggcatt gcctttgatg gcgattttga ccgctgtttt ctgtttgacg
     6901 aaaaagggca gtttatcgag ggctactaca ttgtcggcct gctggcagaa gcgttcctcg
     6961 aaaaaaatcc cggcgcgaag atcatccatg atccgcgtct ctcttggaac actattgatg
     7021 tggtgactgc tgcaggcggc acgccggtaa tgtcgaaaac cggacacgcc tttattaaag
     7081 aacggatgcg caaggaagac gccatctacg gtggcgaaat gagcgcccac cattacttcc
     7141 gtgatttcgc ttactgcgac agcggcatga tcccgtggct gctggtcgcc gaactggtgt
     7201 gcctgaaagg aaaaacgctg ggcgaactgg tgcgcgaccg gatggcggcg tttccggcaa
     7261 gcggtgagat caacagcaaa ctggcgcaac ccgttgaggc gattaaccgc gtggaacagc
     7321 attttagccg tgaggcgctg gcggtggatc gcagcgatgg catcagcatg acctttgccg
     7381 actggcgctt taacctgcgc acctccaata ccgaaccggt ggtgcgcctg aatgtggaat
     7441 cgcgcggtga tgtgccgctg atggaagcgc gaacgcgaac tctgctgacg ttgctgaacg
     7501 agtaatgtcg gatcttccct taccccactg cgggtaaggg gctaataaca ggaacaacga
     7561 tgacaaatct aaaaaagcgc gagcgagcga aaaccaatgc atcgttaatc tctatggtgc
     7621 aacgcttttc agatatcacc atcatgtttg ccgggctatg gctggtttgc gaagtcagcg
     7681 gactgtcatt cctctacatg cacctgttgg tggcgctgat tacgctggtg gtgttccaga
     7741 tgctgggcgg catcaccgat ttttatcgct catggcgcgg tgttcgggca gcgacagaat
     7801 ttgccctgtt gctacaaaac tggaccttaa gcgtgatttt cagcgccgga ctggtggcgt
     7861 tcaacaatga tttcgacacg caactgaaaa tctggctggc gtggtatggg ctgaccagca
     7921 tcggactggt ggtttgccgt tcatgtattc gcattggggc gggctggctg cgtaatcatg
     7981 gctacaacaa gcggatggtc gccgtagcgg gggatttagc cgccggacaa atgctgatgg
     8041 agagtttccg taatcagccg tggttagggt ttgaagtggt gggcgtttac cacgacccga
     8101 aaccgggcgg cgtttctaac gactgggcgg gtaacctgca acagctggtc gaggatgcaa
     8161 aagcgggcaa gattcataac gtctatatcg cgatgcaaat gtgcgacggc gcacgagtga
     8221 aaaaactggt ccatcaactg gcggatacca cctgttcggt gctgctgatc cccgacgtct
     8281 ttaccttcaa cattctccat tcacgtctcg aagagatgaa cggcgttccg gtggtgccgc
     8341 tgtatgacac gccgctttcg ggggttaacc gcctgctaaa acgtgcggaa gacattgtgc
     8401 tggcgacgct tattctgctg ctgatctccc cggtgctgtg ctgtattgcg ctggcggtga
     8461 aactcagttc accagggccg gttattttcc gccagactcg ctatggtatg gatggcaagc
     8521 cgatcaaagt gtggaagttc cgttccatga aagtgatgga gaacgacaaa gtggtgaccc
     8581 aggcgacgca gaacgatccg cgcgtcacta aagtggggaa ctttctgcgc cgcacctcgc
     8641 tggacgaatt gccgcagttt atcaatgtgc tgaccggggg gatgtcgatt gtcggtccac
     8701 gtccgcacgc agtagcgcat aacgaacagt atcgccagct cattgaaggc tacatgctgc
     8761 gccataaagt gaaaccgggc attaccggct gggcgcagat taatggctgg cgcggcgaaa
     8821 ccgacacgct ggagaaaatg gaaaaacgcg tcgagttcga ccttgagtac atccgcgaat
     8881 ggagcgtctg gttcgatatc aaaatcgttt tcctgacggt attcaagggc ttcgttaaca
     8941 aagcggcata ttgatatgag cttacgtgaa aaaaccatca gcggcgcgaa gtggtcggct
     9001 attgccacgg tgatcatcat cggcctcggg ctggtgcaga tgaccgtgct ggcgcggatt
     9061 atcgacaacc accagttcgg cctgctgact gtgtcgctgg tgattatcgc gctggcagat
     9121 acgctttctg acttcggtat cgctaactcg attattcagc gaaaagaaat cagtcacctt
     9181 gaactcacca cgctgtactg gctgaacgtc gggctggggc ttgtggtatg tgtggcggtg
     9241 tttttgttga gtgatgtcat cggcgacgtg ctgaataacc cggacctggc accgttgatt
     9301 aaaacattgt cgctggcgtt tgtggtgatc ccccacgggc aacagttccg cgcgttgatg
     9361 cagaaggagc tggagttcaa caaaatcggc atgatcgaaa ccagcgcggt gctggcgggc
     9421 ttcactttta cggtagttag cgcccatttc tggccgctgg cgatgaccgc gatcctcggt
     9481 tatctggtca atagtgcggt gagaacactg ctgtttggct actttggacg caaaatttat
     9541 tgccccggtc tgcacttctc gctggcgtct gtggcaccga acttacgttt tggtgcctgg
     9601 ctgacggcgg acagcatcat caactatctc aacaccaacc tttcaacgct ggtgctggcg
     9661 cgtattcttg gcgcgggcgt ggcaggggga tacaacctgg cgtacaacgt ggccgtcgta
     9721 ccgccgatga agctgaaccc aatcatcacc cgcgtgttgt ttccggcgtt tgccaaaatt
     9781 caggacgaca ccgaaaagct gcgtgttaac ttctacaagc tgctgtcgat agtggggatt
     9841 atcaactttc cggcgctgct ggggctgatg gtggtgtcga ataacttcgt accgctggta
     9901 ttcggcgaaa agtggaacag cattattccg gtgctgcaat tgctgtgtat tgtgggtctg
     9961 ctgcgctccg tgggtaaccc gattggttcg ctgctgatgg cgaaagcgcg ggtcgatatc
    10021 agctttaaat tcaacgtatt caaaacattt ctgtttattc cggcgattgt tattggtggg
    10081 cagatggcgg gcgcaatcgg cgtcacgctc ggtttcctgc tggtgcagat tatcaacacc
    10141 attctgagtt acttcgtgat gattaaaccg gttcttggtt ccagttatcg ccagtacatc
    10201 ctgagtttgt ggctgccgtt ttatctctcg ttgccgacgc tggtggtcag ttatgcgctg
    10261 ggcattgtgc tgaaagggca actggcgctg gggatgctgc tggcggtgca aatagccgcg
    10321 ggcgtgctgg cgtttgtggt gatgattgtg ctgtcacgcc atccgctggt ggtggaagtg
    10381 aagcgtcagt tttgtcgcag cgaaaaaatg aaaatgcttt tacgggcggg gtgaattgct
    10441 gttttcggtt ccgacaacgg ttgccggatg cggcgtaaaa cgccttatcc gtcctacggt
    10501 tctgtgctcg ggacctgtag gcctgataag acgcgccagc gtcgcatcag gcaaaaaacg
    10561 gcaacgaaca caacaaacac agcctcacct ggcatcaaat ttatgaggaa aaaatgaaat
    10621 tacttattct gggcaaccac acttgcggca atcgtggcga cagcgccatc ttgcgcggct
    10681 tacttgatgc catcaacatc ctcaatccac ataccgaagt ggacgtgatg agccgctatc
    10741 cggtcagttc ttcctggctg ctcaaccgcc cggtaatggg cgatccgctg ttcctgcaga
    10801 tgacacaaca caatagcgcg gcgggcgttg tcgggcgcat taaaaaagtc ctccgtcgcc
    10861 gctaccagca tcaggtattg ctctcacgcg tcaccgacac tggcaagctg cgcaatatcg
    10921 ccatcgccca gggattcacc gacttcgtgc gcctgctgtc aggttacgac gccattattc
    10981 aggtcggcgg atcgtttttt gtcgatctct acggcgtgcc gcagtttgaa catgcgcttt
    11041 gcacatttat ggcgaaaaag ccgctgttta tgattggtca cagcgtcggc cccttccagg
    11101 atgagcaatt taaccaactg gcgaactatg tctttggtca ctgcgacgcg ctgatcctgc
    11161 gcgaatcggt cagccttgat ttgatgaaac gcagaaatat caccaccgca aaagtggaac
    11221 atggcgtcga taccgcgtgg ctggtcgatc accacaccga agacttcacc gccagctatg
    11281 ccgttcaaca ctggctggac gttgccgcac aacagaaaac agtggcaatt accctgcgcg
    11341 aactggcacc gttcgacaaa cgtctcggca ccactcaaca agcgtatgaa aaagcctttg
    11401 ccggggtggt caatcgcatt ctcgatgaag gctatcaggt gattgcgctc tccacctgta
    11461 cgggtatcga cagctataac aaagacgacc gcatggtggc gctcaatctg cgccagcaca
    11521 tcagcgatcc tgcccgttac cacgtagtga tggatgaact caacgatctg gaaatgggca
    11581 aaattctcgg agcctgtgaa ctcaccgtcg gtacgcgcct gcactccgcc attatctcaa
    11641 tgaactttgc tactccggcg attgccatca actatgaaca taaatccgcc gggattatgc
    11701 agcagctggg actgccagag atggcgattg atattcgtca tttactggac ggcagcctgc
    11761 aagcgatggt tgcggatacc ttaggccagc ttccggtgct gaacgctcga tttaacgaag
    11821 ccgtcagtcg cgaacgtcag acgggaatgc agatggtgca gtctgtgctt gagcgcatcg
    11881 gggaggtgaa atgaaggtcg gcttcttctt attgaaattc ccgctgtcgt cggaaacctt
    11941 cgtcctcaat caaattaccg cgtttattga tatgggcttt gaagtggaga ttgtcgcttt
    12001 gcaaaaaggc gatacagaga acacccacgc ggcatggacg aaatacaacc ttgtcgccag
    12061 aacccgctgg ttacaggacg aacctgcggg caaagtagcg aaactgcgcc accgcgccag
    12121 ccagacctta cgtggcattc atcgtaaaaa tacctggcat gcgctcaacc tcaaacgcta
    12181 tggtgccgag tcgcggaacc tgattttgtc tgccatttgt ggtcaggtcg caacaccgtt
    12241 tcgcgccgat gtgttcatcg ctcattttgg cccggcgggg gtaaccgcag caaaactacg
    12301 cgaactgggt atcattcgcg gcaaaattgc caccatcttc cacggtattg atatctccag
    12361 tcgggaagtg ctcaaccact acactcccga atatcaacaa ctgtttcgcc gtggcgacct
    12421 gatgttaccg ataagcgatc tgtgggccgg aaggctgcaa aaaatgggct gccccagaga
    12481 aaaaatcgcc gtatcgcgta tgggcgtgga catgacgcgc tttagcccgc gtccggtgaa
    12541 agcgcccgca acgccgctgg aaatcatatc tgtcgcacgc ttaaccgaga aaaaaggcct
    12601 gcatgtggcg attgaagcct gccgtcagtt gaaagagcag ggcgtggcat ttcgctatcg
    12661 catcctcggc attggcctgt gggaacgacg cctgcgcacc atcatcgaac aatatcaact
    12721 ggaagatgtg gtagagatgc cgggctttaa accgagccat gaagtgaagg agatgctcga
    12781 cgacgcggat gtcttcctgt tgccatcggt aacgggcgcg gatggcgata tggaaggcat
    12841 tccggtggcg ctaatggaag cgatggcggt cggtattccg gtggtttcta ctctgcatag
    12901 cggaattcct gaactggtgg aggccgataa atccggctgg ctgctgcctg agaacgatgc
    12961 tcgcgcactg gcacaacgtc tggcggcgtt tagccaactg gataccgacg aactggctcc
    13021 ggtcgtcaaa cgtgcgtgcg aaaaagtcga acacgatttt aaccagcagg tgattaatcg
    13081 agaactcgcc agcttgctgc aggctttata gaggagaacg tatgccattt aaaacactct
    13141 cccgacgcac cttcctgacg gcaagctcgg cgcttgcctt cctccatacc cctttcgctc
    13201 gcgcacttcc cgcccgacaa agcgttaaca ttaacgacta caacccacat gactggatcg
    13261 cctcatttaa acaagccttc agcgaagggc aaacggtcgt cgtgcctgcc ggattcgttt
    13321 gtgacaatat caacaccggc atcttcatcc ctcccggcaa aacgttacac atccttggaa
    13381 gcctgcgcgg caacggcaga gggcgatttg tcttacagga cggcagccag gtgacagggg
    13441 gagagggcgg cggtatgcat aacattaccc tggatgtgcg tggttctgac tgcaccatca
    13501 aagggctggc gatgagcggc tttggcccgg taatgcagat ttatattggc ggcaaaaaca
    13561 aacgggtcat gcgcaacctg accatcgata acctcactgt cagccacgct aattacgcca
    13621 tcttacgcca gggatttcat aaccagatta tcggtgccaa cattaccaac tgtaagttca
    13681 gcgacttaca gggcgacgcc attgaatgga acgtggcaat taacgacagt gatattttga
    13741 tatctgacca tgtcatcgag cgtatcaact gtaccaacgg caaaatcaac tggggcatcg
    13801 gcataggcct tgcgggaagc acttatgata acaactaccc ggaagaccag gcagtgaaaa
    13861 actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat gttgaaaatg
    13921 gtaaacattt tgttattcgt aatatcaatg cccgcaatat cacgccggat ttcagtaaga
    13981 aagcgggcat tgataacgcg acagtcgcta tttacggttg tgacaatttc gtgattgata
    14041 atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt aaaggcaaat
    14101 atctctcgat accgcaaaat ttccgagtga ataatattca actggataat acccatcttg
    14161 cttataaatt gcgcggcatc caaatctccg ccgggaatgc cgtctccttt gtgtcgctaa
    14221 ctaacattga gatgaagcgt gcctcgctgg agttacacaa caagccgcaa catcttttta
    14281 tgcgaaatat caatgtgatg caggaatcct cagttggacc agcattgagc atgaacttcg
    14341 acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaaa agaaacactg ctgtctcttg
    14401 caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac aggattaatc
    14461 accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg gagtagattt
    14521 gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca atccacaacg
    14581 aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat gctggcgaaa
    14641 aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata atgacgaatt
    14701 taaaagcagt tattcctgta gcgggtctcg ggatgcatat gttgcctgcc actaaggcga
    14761 tacccaaaga gatgctacca atcgtcgaca agccaatgat tcagtacatt gttgacgaga
    14821 ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg
    14881 aaaaccactt cgacacctct tatgagttag aatcactcct tgagcagcgc gtgaagcgtc
    14941 aactgctggc ggaagtacag tccatctgtc cgccgggcgt gatcattatg aacgtgcgtc
    15001 agggcgaacc tttaggttta ggccactcca ttttgtgtgc gcgacctgcc attggtgaca
    15061 acccatttgt cgtggtactg ccagacgttg tgatcgacga tgccagcgcc gacccgctac
    15121 gttacaacct tgctgccatg attgcacgtt tcaacgaaac gggccgcagc caggtgctgg
    15181 caaaacgtat gccgggtgac ctctctgaat actccgtcat ccagactaaa gagccgctgg
    15241 accgtgaggg taaagtcagc cgcattgttg aatttatcga aaaaccggat cagccgcaga
    15301 cgctggactc agacatcatg gccgtaggtc gctatgtgct ttctgccgat atttggccgg
    15361 aactggaacg tactcagcct ggtgcatggg gacgtattca gctgactgat gctattgccg
    15421 agctggcgaa aaaacaatcc gttgatgcaa tgctgatgac cggcgacagt tacgactgcg
    15481 gcaaaaaaat gggctatatg caggcgtttg tgaagtatgg cctacgcaac ctgaaagaag
    15541 gggcgaagtt ccgtaaaggt attgagaagc tgttaagcga ataatgaaaa tctgaccgga
    15601 tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcataa gttttgctca
    15661 ttgaattttc ctgccgttgc cgtttttata aagcctataa aaacaagaag ttacatagct
    15721 catattattc aaatttttcc aggattttcc ttgtttctac ggctgtttgg taagacaatt
    15781 aacgtttgaa tttttcggat ttggcgcgag tggctaacgc tcgccacatc gttgagatgc
    15841 atgcagtgca ctggtagctg ttaagccagg ggcggtagcg tgtattaata cctctattaa
    15901 tcaaatctgg agcagtctat ttcacagcat gctctatagc tatatggaat aaaaaagtga
    15961 agatacttgt tactggtggc gcagggttta ttggttctgc tgttgttcgt cacataataa
    16021 ataatacgca agatagtgtt gttaatgtcg ataaattaac atacgccggg aacctggaat
    16081 cactcgctga tgtttctgat tctgaacgtt atgcttttga gcatgcagat atctgcgatg
    16141 ctgtagccat gtcgcgtatt ttcgcacagc accagccaga cgcggtgatg cacctggcgg
    16201 cagagagcca cgttgaccgt tcaataacgg gccctgcggc atttattgaa accaatattg
    16261 tggggactta tgttctttta gaagcggcgc gcaattactg gtctgctctg aatgatgaaa
    16321 agaaaaaaag tttccgcttt catcatattt ctactgatga agtttatggt gacttacccc
    16381 atccggatga agcaaataat aacgaagcgt taccgctatt tacggaaacg acagcttacg
    16441 cgccaagtag cccgtattct gcgtctaaag cttccagcga tcatttggta cgtgcctgga
    16501 agcggactta tggtttacca accattgtga ctaattgctc gaacaattat ggtccttatc
    16561 acttcccgga aaaacttatt ccattggtta ttctgaatgc tctggaaggt aaggcattac
    16621 ctatttatgg taaaggggat caaattcgcg actggttgta tgtagaggat catgctcgtg
    16681 cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacata
    16741 acgaaaagaa aaacatcgac gtagtgctca ctatttgtga tttgttggat gagattgtac
    16801 cgaaagagaa atcttatcgt gagcaaatta cttatgttgc cgatcgcccg gggcatgacc
    16861 gccgttacgc cattgatgcc gataaaatta gccgcgaatt gggctggaaa ccgcaagaaa
    16921 cgtttgagag cgggattcgt aaaacggtgg aatggtatct ggctaataca aattgggttg
    16981 agaatgtgaa aagcggtact tatcagtcgt ggattgaaca aaactatgag ggccgtcagt
    17041 aatgaatatc ctgcttttcg gcaaaacagg gcaggtgggt tgggaactgc agcgtgctct
    17101 ggctcctctg ggtaacttga ttgcgctcga tgttcactcc actgattatt gtggagattt
    17161 cagcaacccc gaaggtgtgg ctgaaaccgt caaaaaaatt cgccccgatg ttattgttaa
    17221 tgctgctgct cacactgcag tagacaaagc ggaatcagaa ccgaatttcg cgcaattact
    17281 taacgcgaca tgcgtcgaag cgattgcaaa agcagctaat gaagtcggag cctgggttat
    17341 acactactcc actgattatg ttttcccagg caacggtgac acgccatggc tggaaacgga
    17401 tgcaacagca ccgctaaatg tctacggtga aaccaagcta gctggggaaa aagcgttaca
    17461 agaacattgc gcaaagcatc ttattttccg taccagctgg gtatacgctg gtaaaggaaa
    17521 taactttgcc aaaacgatgt tgcgcctggc aaaagaacgc gaagagttgg ctgtaataaa
    17581 cgatcaattc ggcgcaccaa cgggggctga attactggct gattgcaccg ctcatgccat
    17641 tcgcgtggcc gcaaataaac cagaagttgc tggtttgtac catctggttg ctggtggtac
    17701 aacaacctgg cacgattatg ccgctctggt atttgaagaa gcgcgcagag cggggatcaa
    17761 ccttgctctt aacaaactta atgccgtgcc gacaacggcc tatcccacac cagcacgtcg
    17821 tccccataat tctcgcctca ataccgaaaa atttcagcag aactttgcgc ttgtcttgcc
    17881 tgactggcag gtgggcgtga aacgtatgct caacgaatta tttacgacca cggcaattta
    17941 acaaattatt gcatctcgct catgatgcta gagcgagatg aattaaaagg aatggtgaaa
    18001 tgaaaacgcg taaaggtatt attctggctg gtggttccgg cactcgtctt tatcctgtga
    18061 caatggctgt gagcaaacaa ctgctaccga tttatgataa accgatgatt tattatccgc
    18121 tttcaacgct tatgttggcg ggtattcgag atattctgat tatcagtacg ccgcaggata
    18181 caccacgttt tcaacaattg cttggtgatg gaagccagtg ggggcttaat ctgcaatata
    18241 aagtacaacc gagtccggat ggtctggcgc aagcatttat cattggtgaa gaatttatag
    18301 gtggtgatga ttgtgcgctg gtacttggtg ataatatctt ctatggtcac gacctgccga
    18361 aattaatgga taccgctgtt aacagagaga gtggcgcaac ggtttttgcc tatcacgtaa
    18421 atgatcctga gcgctacggt gtggtggaat ttgatgataa cggtacggca attagtctgg
    18481 aagaaaaacc gcaggaacca aaaagtaact atgcggttac tgggctttat ttctatgaca
    18541 atgacgttgt agaaatggcg aaaaacctta aaccatctgc acgtggcgaa ctggaaatta
    18601 ccgatattaa ccgtatttat atggaccaag gacgtttgtc tgtcgctatg atggggcgtg
    18661 gttatgcatg gctggacacg gggacacatc aaagtcttat tgaagcaagc aacttcattg
    18721 caacaattga agagcgtcag gggctgaaag tttcctgccc ggaagaaatt gctcaccgta
    18781 aaggctttat tgatgctgag caagtgaagg tattagccga gccgctgaag aaaaatgctt
    18841 atggtcagta tctgctgaaa atgattaaag gttattaata aaatgaacgt aattaaaact
    18901 gaaattcctg atgtgctaat ttttgaacca aaagtatttg gtgatgagcg gggctttttt
    18961 atggaaagct tcaatcagaa agttttcgaa gaggctgtag gacgaaaggt tgagtttgtt
    19021 caggataacc attctaaatc aactaagggt gtgttacgcg gactgcacta tcagctggaa
    19081 ccttatgctc aaggaaaact ggtacgctgt gttgtcggtg aagtttttga cgtagctgtt
    19141 gatattcgga aatcatcacc aacatttggc aagtgggttg gtgtaaattt gtcggcggag
    19201 aataagcgtc aattatggat accggaagga ttcgctcatg ggttttgcgt tcttagtgat
    19261 gaagcagaat ttgtctacaa aacaaataat ttctattcaa aaatgcaaga gcgtgggatt
    19321 ttatggagcg acaaaagtat aaatatagaa tggccggttc aaaatccatt gctttctgat
    19381 aaagatatta atggtcaaaa atttgtagat gctgatta
//
LOCUS       DAZXUK010000049        19375 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZXUK010000049 DAZXUK010000000
VERSION     DAZXUK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19375
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.049"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="ACH3L8_001432"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="ACH3L8_001432"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFT4084608.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSSRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="ACH3L8_001433"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="ACH3L8_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFT4084609.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACH3L8_001434"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACH3L8_001434"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFT4084610.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTLSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="ACH3L8_001435"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="ACH3L8_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFT4084611.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="ACH3L8_001436"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="ACH3L8_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFT4084612.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="ACH3L8_001437"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="ACH3L8_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFT4084613.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="ACH3L8_001438"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="ACH3L8_001438"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFT4084614.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="ACH3L8_001439"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="ACH3L8_001439"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFT4084615.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="ACH3L8_001440"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="ACH3L8_001440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFT4084616.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="ACH3L8_001441"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="ACH3L8_001441"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFT4084617.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="ACH3L8_001442"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="ACH3L8_001442"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFT4084618.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="ACH3L8_001443"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="ACH3L8_001443"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFT4084619.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="ACH3L8_001444"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="ACH3L8_001444"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFT4084620.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="ACH3L8_001445"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="ACH3L8_001445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFT4084621.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="ACH3L8_001446"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="ACH3L8_001446"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFT4084622.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /locus_tag="ACH3L8_001447"
     CDS             15215..15634
                     /locus_tag="ACH3L8_001447"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFT4084623.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="ACH3L8_001448"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="ACH3L8_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFT4084624.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="ACH3L8_001449"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="ACH3L8_001449"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFT4084625.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4772 a         4855 c         5117 g         4631 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcagtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cagggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaata atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgccg
//
LOCUS       DAZXUK010000050        19201 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZXUK010000050 DAZXUK010000000
VERSION     DAZXUK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19201
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.050"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..271
                     /locus_tag="ACH3L8_001450"
     CDS             <1..271
                     /locus_tag="ACH3L8_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFT4084626.1"
                     /translation="SGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITYRREGYEYDCV
                     MPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            268..2016
                     /locus_tag="ACH3L8_001451"
     CDS             268..2016
                     /locus_tag="ACH3L8_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFT4084627.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            2305..2583
                     /locus_tag="ACH3L8_001452"
     CDS             2305..2583
                     /locus_tag="ACH3L8_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084628.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            2580..2885
                     /locus_tag="ACH3L8_001453"
     CDS             2580..2885
                     /locus_tag="ACH3L8_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084629.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            2895..2969
                     /locus_tag="ACH3L8_001454"
                     /pseudo
     CDS             2895..2969
                     /locus_tag="ACH3L8_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3044..3157
                     /locus_tag="ACH3L8_001455"
     CDS             3044..3157
                     /locus_tag="ACH3L8_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFT4084630.1"
                     /translation="MSRVGISEPHHAAGAIRLFLRHLKRYGALLAFRHGPA"
     gene            3198..3422
                     /locus_tag="ACH3L8_001456"
     CDS             3198..3422
                     /locus_tag="ACH3L8_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084631.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            3723..4880
                     /locus_tag="ACH3L8_001457"
                     /pseudo
     CDS             3723..4880
                     /locus_tag="ACH3L8_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            4920..5492
                     /locus_tag="ACH3L8_001458"
     CDS             4920..5492
                     /locus_tag="ACH3L8_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFT4084632.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            5494..6705
                     /locus_tag="ACH3L8_001459"
     CDS             5494..6705
                     /locus_tag="ACH3L8_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFT4084633.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            6702..7040
                     /locus_tag="ACH3L8_001460"
     CDS             6702..7040
                     /locus_tag="ACH3L8_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFT4084634.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            7037..7333
                     /locus_tag="ACH3L8_001461"
     CDS             7037..7333
                     /locus_tag="ACH3L8_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFT4084635.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            7333..7773
                     /locus_tag="ACH3L8_001462"
     CDS             7333..7773
                     /locus_tag="ACH3L8_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFT4084636.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            7757..7939
                     /locus_tag="ACH3L8_001463"
     CDS             7757..7939
                     /locus_tag="ACH3L8_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084637.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            7995..8321
                     /locus_tag="ACH3L8_001464"
     CDS             7995..8321
                     /locus_tag="ACH3L8_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFT4084638.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            8305..9966
                     /locus_tag="ACH3L8_001465"
     CDS             8305..9966
                     /locus_tag="ACH3L8_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFT4084639.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            9980..10261
                     /locus_tag="ACH3L8_001466"
     CDS             9980..10261
                     /locus_tag="ACH3L8_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084640.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(11058..12518)
                     /gene="phoQ"
                     /locus_tag="ACH3L8_001467"
     CDS             complement(11058..12518)
                     /gene="phoQ"
                     /locus_tag="ACH3L8_001467"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFT4084641.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(12518..13189)
                     /gene="phoP"
                     /locus_tag="ACH3L8_001468"
     CDS             complement(12518..13189)
                     /gene="phoP"
                     /locus_tag="ACH3L8_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFT4084642.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(13358..14728)
                     /gene="purB"
                     /locus_tag="ACH3L8_001469"
     CDS             complement(13358..14728)
                     /gene="purB"
                     /locus_tag="ACH3L8_001469"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFT4084643.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(14732..15373)
                     /gene="hflD"
                     /locus_tag="ACH3L8_001470"
     CDS             complement(14732..15373)
                     /gene="hflD"
                     /locus_tag="ACH3L8_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFT4084644.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(15409..16515)
                     /gene="mnmA"
                     /locus_tag="ACH3L8_001471"
                     /gene_synonym="trmU"
     CDS             complement(15409..16515)
                     /gene="mnmA"
                     /locus_tag="ACH3L8_001471"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFT4084645.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(16569..17030)
                     /gene="nudJ"
                     /locus_tag="ACH3L8_001472"
     CDS             complement(16569..17030)
                     /gene="nudJ"
                     /locus_tag="ACH3L8_001472"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFT4084646.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(17040..17693)
                     /gene="rluE"
                     /locus_tag="ACH3L8_001473"
     CDS             complement(17040..17693)
                     /gene="rluE"
                     /locus_tag="ACH3L8_001473"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFT4084647.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            17865..19115
                     /gene="icd"
                     /locus_tag="ACH3L8_001474"
     CDS             17865..19115
                     /gene="icd"
                     /locus_tag="ACH3L8_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFT4084648.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         4985 a         4711 c         5155 g         4350 t
ORIGIN      
        1 cagtggcaac actcacccta aatttacgcg cggtgatatt taccgcgacc agtacggcgg
       61 catggtaacg attaaaggcg tggcagaacg gcgcatcacc taccgccgtg aaggttacga
      121 atatgattgc gtgatgcctg tttatcaatt ccggcgtgat ttcacgctgg tacaggcagc
      181 gccccgcagt aaaccaacca gcagggagaa agcacgcgcc aatattcagg aaataaaaaa
      241 gatgcttaac gtattcaggg gtaaaaaatg aaactggcac cgaacgtaaa aaaacagcca
      301 cgcggaataa aacacaaaga cacagaggta attatctttg cgggtagtga tgcctggtcg
      361 cacgcgaaac aatggcagga gcaggatggc cccgcatccg gcgataatgt gccgcctgtg
      421 tggcttgggc caaatcagct tgccgaactt gatgcactga aaattgttcc ggatggaaaa
      481 aaacgcgtaa ggctgtacca ggccggagaa ctggatttgg tggagaccaa aaagattggt
      541 cagaagctgg cggcggcaga tattcaggac gcaaattttt accccgaagg aatgcacgtc
      601 cagaagtgtg aaaactggcg gcgctatctg aatgctgagc gtgaaaatat tgccgcaggg
      661 cttaccatgc cggagcagaa aaatacgcaa ctggcacaaa tggcagacag tgagcgcgcg
      721 cagctgcttg ccgagcgctt tgatggcgtt tgtgtgcatc aggaaagtga aatcgttcac
      781 gtatggcgcg gcggggtatg gtgtccggtc agcacaatgg aacttagccg cgaaatggtg
      841 gtgatctatt cagagcacag ggccactttc agcaagcgcg taatcaataa cgccgtggaa
      901 gcgttaaaag ttattgccga accaatgggc gagccgtccg gcgatttgct gccgttcgcc
      961 aatggtgcgc ttgacctgaa aacgggggaa ttttccccgc acacgccgga gaactggatc
     1021 accacgcaca acggcattga gtacacgcca ccagcaccag gggagaacat ccgcgataac
     1081 gcgctaaact ttcataaatg gcttgagcac gcagccggaa aagaccagcg caagatgatg
     1141 cgtatatgtg ccgcgctgta catgattatg gcgaaccggt acgactggca gatgtttatt
     1201 gaggccaccg gagacggcgg gagcggtaaa agtacattca cacacatagc cagccttctg
     1261 gcagggaaac agaacacggt aagcgctgaa atgacatcgc ttgatgatgc tggtggacgt
     1321 gcgcaggttg tcgggagtcg tcttatcgtc ctggctgacc agccgaaata tacaggcgaa
     1381 ggaacgggca tcaagaaaat cacgggcggt gaccccgtgg aaattaaccc gaaatacgaa
     1441 aagcgtttca cggctgtaat cagggcggtg gtgctggcga ccaacaataa cccgatgata
     1501 ttcaccgaac gggccggagg tgtggcacgt cgtcgcgtga ttttccgttt cgacaatatc
     1561 gttagtgagg ccgaaaaaga cagggagcta ccggaaaaga ttgcggctga aatccctgtc
     1621 attatccgcc gattgctggc gaactttacc gactctgaga aagcacgggc tttactactg
     1681 gaacagcgtg acggtgatga agctctggca ataaagcaac agacggatcc ggttattgag
     1741 ttttgccagt tcctgaattt tctggaggaa gcacgcggcc tgatgatggg cggcggtggc
     1801 gattcagtga agtacacgac cagaaacagc ctttaccgcg tctatctggc gtttatggcg
     1861 tacgcaggca ggagcaaacc gctaaacgta aatgactttg gcaaggctat gaagccagcc
     1921 gcgaaagttt acggacatga atatattacg cggaaagtta agggagtaac gcagactaac
     1981 gcaataacaa cagacgagtg cgacgcgttt ttataatttt ttgtaattgc tgtctaccct
     2041 gtctacctga gtaaagaaaa atacatttaa ttcagtgcat taagttaggt agatagcctt
     2101 tttttactgt ctacccacta tctaccctct ctacctgatt ttacctgaat cagacaggga
     2161 ggtagacacg gggtagatag tggataaaag cactctaccc cgctgaaagc agcgccatta
     2221 ctggcatgag ggccactaag gtagataagg tagacaaggg gaggcacaac tcaaaacttt
     2281 ttaaacgagg gggtaaaaat aaatatgcac acttcaggga aatttaataa atcattcaaa
     2341 aaacgcagag acagaacaga accgaaatat cgcgcgttag acatgacaga gcacgcttta
     2401 aaggtggcaa tcagaacgat agaccgccac gcgggggaag gatacgcgaa agcacatccc
     2461 gaactgataa gcgcattcat gaccacgacg gcggcaaatt ttgccacgct gacagagcgg
     2521 gagattgccg aagcggaaca ggtaacaaca atcaacgtta aaaccggaga ggtggaatca
     2581 tgagcgaacc aaaatttggt gaaaaatttt acaagcacaa cggacggatc acgattctgc
     2641 aaataagcgc agctacacca ggctggtggg ttgaaacaga cgaaggatcc tcacccgtgg
     2701 catcatggga gctttgtgtg cttagttacc ctgaccgcga tgtgtatcag gatattctgc
     2761 ctgtgatatc cactgacaaa ggcatgaaac ccgtagacat caaaaacatg gggtttcagt
     2821 gcgtaatgct cactgaaaaa atgatggaag agatgaaaaa gaacaatgcc ggatcgcttc
     2881 actgaggtgg caatatgaca gcacaaattg cagcttacgg gcagctggtg ggcgacccgc
     2941 tggtaaaaca gagcagcaag ggcacaccgg agccgccaac ggagaactac gcagacctga
     3001 gaaatgcagt aaatcgcact tacgcagaca tggggctgag caaattagca gagtaggaat
     3061 atcagaacca caccacgcag ccggagcaat ccggcttttt ttgcgtcatt tgaaacggta
     3121 tggagcacta ctggcttttc gtcacggtcc ggcatagtta ctatctgaaa caaacagaca
     3181 caacagagga aaacgcaatg ccgatgaaat ttgatgagat acttaaacag cgtgataaat
     3241 accatgctga caacatggag acgatgaata tcactgatta ccgcgcattt ctggaaacgg
     3301 gggcgctgat tgaaaaggat catcatggtt ttgttcgttg cgcgctatcc ggtgaaatgc
     3361 tggcagtaaa tcctgaacag acagatgcat tgatagaatt tctgaaaggg atcagagact
     3421 gagcacccat acagcacata cagccggagc aatccggctt tttgttattt tttgtaaatt
     3481 atttgttcgt ggttgttcca cgttgttcac tgagcggatc ggcatatttt acccgaactg
     3541 aatcatgatt attctcgccc gtggtgccag gacgctgggg ccactttccc gcctgttaat
     3601 gtgctcgcca atattcatta ccaggcggga aaacgatcgg tgcgattact gatttcctta
     3661 tgaaaaacgg ttgagtgttt gccgcgtcct ggagttcctt acttaacccc aggacttttt
     3721 ttatgcagag aataatcgaa ttacgccagc agaaaaccgc cattaaaaat cagatgcgcg
     3781 acatgctgga gaacgctgaa aaagaaaacc gcagtcttaa cgatgctgag ggcgcaaaat
     3841 ttgacgaatt acgcgctaaa gctgaatccc tcgataaaga catttcccgc cttgaagcca
     3901 ttgcagacga agagcgcagc aagccaggta aaagcagcca gaccactgac cccgcagaac
     3961 tacgtaacta cattctgacc ggtgaaaccc gcgcattaag tacaggcgtt cccgctgatg
     4021 gtggttatac cgttatccct gaactgaaca ccgaaatcat gcgaatgctg gcggatgaat
     4081 ccaccatgcg tcgcatctgt accgtgaaga aaatcagcag caacgagttt aagcagcttg
     4141 tttccgctgg cggtgcgacc gttaaccacg gtgaagaggg taaggcacgc gaacagacca
     4201 gcaccccgca gattaacgag gtgagcatta agctgtatcc ggtctatgcg tacccgcgca
     4261 ccacacagga aatcgtggat ttttccgatg tggacatcct ttcatggctg acgggtgaga
     4321 ttggcgacac cttcacggaa accgaagaaa gcgatctggt tgtgggcgac ggtgacaaaa
     4381 aagcaaaagg ttttttatcc gtaccccgtg cagagaagaa cgacaaagag cgtgattttg
     4441 gtacgttgca ggtaattaaa ccttccgaat ctctggcgtg gacatctgcg gacccgctga
     4501 tcgacctgaa atttgcatta cgtaaaaaat accgcaaaaa cgcggtctga gtggttaact
     4561 ccacgacggc ggcaaaactt cagaaggtga agaacgcgaa cggtgattat atctggcgcg
     4621 accgtttaca ggcgggtgat cctgatacgt tgctgggcct tccggtcgaa tatctggagt
     4681 ttatgcctga taacgttatt gccctgggtg acttcaaacg cggttactac attgttgatc
     4741 acgaaacagg tgttcgcacc agaccggaca acctgacaga accaggattc atcaaaattt
     4801 tcacgcaaaa atatttaggc ggtggcgtgg tggattcgaa cgcgatcaag attctggcac
     4861 tgccggaaga cgacgattaa cagcatacag aaggggctaa aaagcccctt tagtgtttta
     4921 tgggtgaaaa aattatgaag agtatggaaa tccggtcatc ggaaatcacc accagcggag
     4981 ccggtacgct gacgggctac gttgttcgct gggataagct ttcagaactg ttatggggtg
     5041 agttttacga aaaattccag cggggggcgt ttactgaatg gcttgcggcg ggtaatgacg
     5101 ttcgcggcct gtatgagcat gaccacagca tgttactggg gcgcacccgt tccggcacgc
     5161 tgaaactgga agaggacgaa acagggttac gctttgaact gaccccaccg gataccagta
     5221 cagggcgcga cgttatcgaa ctggttaagc gtggggacat ctcggggatg agctttggct
     5281 ttcgctcccg taaggatgta tgggatacca caacagatcc atgcgtgcgc accgtgctgg
     5341 tggcggaact gtacgaaatt accgttacat cggtaccggc ttaccccgat tccggcgtgg
     5401 agctggcccg ccgttccctg tatgagcagc accccgaaaa aatgccgcgt gcggataatc
     5461 gccgctggtg ggcggattta gcgggggtgt gatatgtggc ctttcagacg aaaaaaagag
     5521 cagcgcagca tgacgcttga tgaatttatg gcgctggccg gcacatcgaa cacgggggcg
     5581 ggtgagtacg tatcatctgg gacagcggaa tcactccccg ccgtcatgaa cgccgtcacg
     5641 gtcatctctg acgcggtggc taccatgccg tgttacctgt acctggtacg caatgagaag
     5701 gggaaggagg cccgcgaatg gcttgattct catccggtcg atcacatcct caacgagcgc
     5761 ccgaacgcgt ggcagacacc ctaccagttt aagcgaatga tgatccgcta ctgcctgtta
     5821 agtggtaatg cttatgcggt tattcagtgg gggcgtgatg gttttccggc ggctttacat
     5881 ccttacccgc cgcagtcggt gaacgtggag cagacaggtg atcataactg gcgctactgc
     5941 atcactgacg cctacaccgg aaacatccgc aactatttac cgtgggaggt tctccacctt
     6001 cgttactcca cggatgacgg ttttatgggg cgctcacctg taaccatctg ccgcgaatcg
     6061 ctggggcttg ggctggccca acaacgccac ggcgcgagcg tgatgcgtga tggcatgatg
     6121 gcggcagggg ttatcacgtc aggcgaatgg ctggacggcg tgaagggcaa acaggcatta
     6181 gccgcactgg aacgctacaa aggggccaga aacgccggaa aaacgcccat ccttgaaggg
     6241 gggatgagct atcagcagct gggcatgagt aatcaggatg ccgaatggct ggcctcccgt
     6301 cgcttcacca ttgaagacat cgcccgaatg ttcaacgtct cgccgatttt tttgcaggaa
     6361 tacagcaaca gcacctacag caacttcagc gaggcaagcc gcgcatttct caccatgacg
     6421 atgcgcccgt ggctggcgaa ctttgagcag cagataaaaa acgccttgct ggtggcctcg
     6481 cctgtacctg gtatccggta tcaggtggag tttgacagcg cggacctgtt acgggccaca
     6541 cctggcgaac gctttgccac ctatgagcgc ggcataaaat ccgacgttat gtgtccgaac
     6601 gaagcccgcg aacgtgaagg gctgtcaccg cgtgatggtg gtgatgagtt cagccaggca
     6661 tggaaacagg aagtaaaaat cagcgaggga gaaaaaccgg aatgaacata gggcgactgc
     6721 gtgacaggat aacgattcag accctgaaac agaccaggga tataaccggc gaaatactcg
     6781 aaacgtggga ggacggtcac acactctggg caagcgtgaa catggtcagc agcaaggagg
     6841 ccatttcatc gggcgcagag ctggcgattg gtaccgtaag gatctggata cggtaccgga
     6901 aggacatcaa cgccaccagc cggataaagg tcagtacggg gccgctggcg gggcgtgtac
     6961 tgaatatcat cgggcagccg ctgccggatg ccgccaggac acgccttgaa attctttgtc
     7021 gtgagggcgc ggaaaaatga cagaagaact tatcaccctg gaagaagtga aactccattg
     7081 ccgcatcgat ggcgacgatg aagaccagtt aatcagcgga tacattgccg catcgcttga
     7141 ggcgtgccag atacacatag gcaggcgctt tgatgacggg ctggcgttca cgccagccat
     7201 aaagattggc tgcatgatgt ttatcgctca cctgtatgaa aatcgccaga ttgtcgcgga
     7261 taacgcaaaa acacgcgtac ccatgacgat aggcgcactc tggacggctt accgtgatgt
     7321 gggggggtac tgatgccgtg gcaaccatta agacgatgca cagaaccagg ctgtaacagg
     7381 cgcgttaaat ccggcaagtg tgaggagcac aggcgggcgg catggcgtgc agaggatgcc
     7441 agacgaggac accgccgcgc gcgtggatat tccagacagt gggacaaata ccgcgccatg
     7501 tatctgagta aaaacccgtt atgcgtgcga tgccttgaga aggggatata tacgcccgcc
     7561 gtggtggtgg atcacattat cccgattaat ggcggtgatg atgttctctt ctggcccgaa
     7621 tggaaccatc aaccattgtg ccaggcgtgt cataaccaga aaacgaaatg gcttgatccg
     7681 gcaacaaaaa gcaagcgtgc cgcaggtgga tttcgtgaag aggaagaacg ggctgctaac
     7741 cgcaataact ggatgtatga cgctgatgaa tgagcgggaa caaaaccgcc ttatccgtgg
     7801 acttataagg cagcgtgacg catggaagac acaggaaaca gggcataaag ataaagcgtc
     7861 aggacgcgca gaacgcatca cagcgaagcg attaaccgac cgtgaccgcg aggttatgga
     7921 atgtttccgc aatcgctgat gaggctgtat gacggggtgg ggggcgtttt caggacaaac
     7981 ccgaccccgc ctacctggat gatatcgccg tgaagcagtg gcgggaaaaa tcgcggcagc
     8041 ttgcggaacg gggagacctg acccccgccg actggagcaa tctggaactg tattgcgtca
     8101 actactccat ttaccggaaa gccgttgcag accttgcggc gcgcgggttc agcattgtta
     8161 acagtcaggg cggcgagagc agaaaccccg cattaagcgc aaaatccgac gctgaaagag
     8221 tgatgataaa aatggcctcc ttgctcggtt ttgacccgat aagccgccgt aaaaatccac
     8281 cggaaacaga agaagaggac gagcttgacc gcctggaata agtacgcaga agacgtaaaa
     8341 acgggcaaaa ttccggcctg taaacggtta aaacaggctg ttaaacggta cttttcggac
     8401 cttaaaagcc ccctttacac gttcgatcgt gaggttgtgg agcggtttat tgccttttcc
     8461 agggtgtgcc cgcacgtaaa aggaccgatg cgtggcagac ccattgagct ggagccgtgg
     8521 cagcagttcg cctttgcgtg catcctcggc tttaaggtta aggccaccgg acggcgcaaa
     8581 tacacgagcg cctttatcga agtgccgcgc aaaaatgcga aatccacgac cgccgcgatt
     8641 ctggctaact ggtttctgat tatggagaac ggtcagcagg atatctacac cgcagcggtg
     8701 agccgtgacc aggcgcggat cgtgtttgat aatgcgcgtc agatgtgcct tttatcccga
     8761 ccgttacgca ggcgggtgaa tattcaggcg cataaggtga tacacccgaa aagcaacagc
     8821 ctgttaaagc cgctggcagc aaaagcggca accatcgagg ggacaaaccc cagtctttcc
     8881 atcgtggacg aataccacct acaccccgat aacggcgttt attccgcgct tgagctgggg
     8941 atgggcgcac gtcccgaagg gctgttattt gccattacca catcgggcag taacgtcgtt
     9001 tcagcctgta aacagcacta tgattattgc tgccagatcc tggacggcga agaggttaac
     9061 gattcaattt ttgtactgat ttacgaactg gacgacgaaa acgaggttga tgatccggcg
     9121 atgtggataa aggcgaaccc taatattgat gtttccgtgg atcgtgaaaa actggcctca
     9181 accatccaga aagcgcgggg tattccgtcg cagtgggtgg agatgatgac aaagcgattt
     9241 aatatctggt gtcaggggac taccccgtgg atgggtaatg gtgcatgggc tgagtgtgcc
     9301 ggaacgttca cggaggaaga tttacacggt caggagtgtt acgcggggct ggacttatca
     9361 tcaaccagcg atatttccag cgtatgctat gcctttccgg tcggtaaaac cattatgctg
     9421 atttcccgtc attatctgcc ggagttccag ctacagaacc ccgccaataa aaaccgcgct
     9481 gtctatcgcc agtgggctaa agcgggctgg atacgcacaa cgccaggtga ctgcattgat
     9541 tacgaccgga tcagagatga catcatgcag gatgcggaga aatttaatat caggctggtg
     9601 ggctttgata cgtggaacgc cacgcatctc aggacgcaat tacagggggc tggttttgag
     9661 gtggagccgt tcccgcaaac ctacctcaga ttcagtccgg cggcgaaatc gttcgaagtt
     9721 tttgttaacc gcagggtgat tgtgcatcgt ggcgatccgg tgttgtcctg gtcgatgagt
     9781 aacgtcgtga tgcagagtga cgcgaacgcc aatatcaagc cgaacaagaa aaaatcaccg
     9841 aacaagatag acccgagcgt agcggcgctg atggcgtttg gtacattcca ggcagagcat
     9901 gaggattttg cttttgatat aagcgacagc caccgccaga aactggaaga atttagcggg
     9961 gtgtaatgag gtcagcagca tgacagaagc cgaaatactg cgattaatcc gccgcgtctc
    10021 tggaatcagc cagcaggctg acgaacagac cacgcagccg gacagcgtga cagccgaaaa
    10081 ttatgcgcgt gttgttgctg aggtgatgcg ccgtgatggt atccagctta atgatgcgga
    10141 tatgcgcgac atacggatcc gcgttctgga aatgctggcc tacaatcgcc gcgttgaact
    10201 gtatcgggag aaggagaaaa tcacgtacca ctggaagaag ccggagcggt tacggcggta
    10261 actcactgag attaaagcaa agcgcaaaat tgcgctggct acatccctca catctgagga
    10321 ctgttaacca gcaggccgga atatattcag aaactacgaa attttcgcag tttaattact
    10381 cgtcagctta atcgctaacc cgctctgata ttttcggaaa cctcaatttg aggaagtcgg
    10441 cgaggtaact gtttgatatt cccgatagcg caaagatgcg cggtcgggca gaatgaccac
    10501 cacctcaatc gtgacggaga gtgacaacca gtgacacacc gtgacaaaaa gacggtttaa
    10561 ccgttcatta aaaatcgacg tgattttgtg ctttccggat gacgtggcca acgtcatttt
    10621 ttagcgaaaa attctgatta aggtcactgg ctgaacggtc ataatgacca cgcagaccat
    10681 cgaggcaacc atagcgatca tcgttacggt tggagctttt ccgccattgg tggaaaaggt
    10741 gcacatctgg atcatcatta cggtgatcaa gggtatgtaa aacccaccag cctgattaaa
    10801 ggttaaccgg aaaaaaaatc cggtagcccc gttatgggga tccccatatc gacattaacg
    10861 cccctcatga attggtgcgc ttccccctgg aaagattacc cgcctgtata tttcttgtgt
    10921 ctatttgttc cacgttgttt catacagtgc accgaacggt gtagttaatg gtgtagtcaa
    10981 tttgcgattt ttggcacttt ttcaatgaaa attgtttgat cgaatcaatg acttgatgta
    11041 gtggtaaaag gacatattta ttcatctttc ggcgcagaat gctggcgacc aaaaatcacc
    11101 tccatccgcg caccgcccag catgctctct ccggcgacga ttttaccctc atattgctcg
    11161 gtgatttcgc gggctaccgc cagccctaca ccttgcccag ggcgtaaagt atcaacccgt
    11221 tgaccacggt cgaaaatgac ctctcgcttg cttaatggaa taccggggcc atcatcctcg
    11281 accacaatat agagatgctc gtcggtttgc cttgcagaaa tttcgacaaa ctcgaggcaa
    11341 tatttacagg cattatccag cacgttgccc atcacctcga caaaatcgtt ctgctcaccg
    11401 acaaagctga tctctggcga aatatcgaga gagatattga cccctttgcg ttgatacact
    11461 ttgttcagcg ctgaggtgag attgtccagc agtggggcga ccggatgcag ctcgcggctg
    11521 agcaatgtcc cgccgcgcat actggcacga tgcaggtagt agccaatttg ctgtgaaatg
    11581 cggctgattt gctccagcat taccggctca gcatcactga cgctcatctt ttcactacgc
    11641 agagaacgca gcgtactttg cagcaccgcc agtggcgttt tcagactatg ggtcaggtcg
    11701 gtgagcgtcg tacggtattt gtcgtaacgt tcgcgttcac tttttaacaa tcggttcagg
    11761 tttcgtacca gactggtcag ttctcgcgtt gtggctggat tgagcaattc gcggttatgt
    11821 tcttccagtt cgcggacttc ttttgccagg gcttcgatgg ggcgtaaact ccaccaggcg
    11881 gcgacccaca gcagcgggat cactaacagc agattggctg agagcacata gataaaccag
    11941 ctccagacca tataggaact ttttagctcc accggaatgg tatccaccac cacaatggtt
    12001 aattttggca tccgcgatgt tgccgggtag acgtttaccg ccaccgagtg ggtcatctcc
    12061 gcgtcgtcat catcttcccg cacttcctgc aactgttgct gtatcgaatg atctccactc
    12121 agcaagaggc tggtatcgtt aacatccgct tcaatttcat gaaaaccatt cgatttcagc
    12181 cagtcaggct ggatcatttt catcagccag ggcacgtcac gttgcgccca taaaagctgc
    12241 ccgttctcat cataaattag cgtcatggtg gggctttgct tgtcgatatt ttcgggtaac
    12301 tcgacatgca acttattgtt ttcccactgc gcaagggtat agaacagatt gctctcgcca
    12361 cgtaacagcc gaaacgtagt tttatcgaaa ctgacgctat aaccgatcag cgcgaccatt
    12421 ccgtaggcaa gcgaaagcac cagtactacc gctgccgttg ccaacagaaa acgtacccgc
    12481 agcgagagcg ggaaaaaaag acgcagtaat tttttcatca gcgcaattcg aacaggtagc
    12541 cctggccgcg aacggtggta atgacttctt gcggatattg tgcctgaatt tttttgcgca
    12601 gacgtcccat cagtacatca atggtatggc tttcccgcag ctccgcatcc ggatagagtt
    12661 gtagcattaa cgaatctttg ctgaccactt tgccattatt gcgtatcaac gtttccataa
    12721 tggtgtattc gaacgcggtc agtttgatca cttcgtcatt aatagataat tcacggcgag
    12781 agagatcaac ctgaaacggg gggagcgaaa tgacctgtga agccagaccg ctattacgcc
    12841 gcattaatgc ctgcattcgc gccatcacct cttcaatatg aaacggttta gtcacgtaat
    12901 catcagcacc ggcacttaat acttcgactt tgtcctgcca gctttcacgg gcggttaata
    12961 ccagaatcgg cagtgaaaca tcgttgctac gccagcggcg aatcagtgac agaccgtcct
    13021 cgtcaggcaa tccgagatcg acaatcgcaa tatccggtaa atgttcattg agataataat
    13081 cggcttcttt ggcatcttct gcgtcatcga cctgatgacc agcatcctga atctgaactt
    13141 taaggtggtg acgtaacaac gcattgtctt caacaaccag tacgcgcatt tttatttctc
    13201 cctgtcttaa ttattaaaat agtgtaacgc gattatgtgt ttatgggggt aaacattaaa
    13261 taaaccagcg gggaggggag gtaaagtgaa aaaagaaaaa gtggataatc ttaataagca
    13321 ggccggacag catcgccatc cggcactgat ttgaggctta tttcagctca tcaaccatcg
    13381 tgatagctcg accaatatag ttagccggcg tcatcgcttt caggcgggct ttctcttctt
    13441 ctggcaacgc cagaccatcg ataaactgct tcatgccttc ggcgtcaacg cgcttaccgc
    13501 gagtaagctc tttcagcttc tcgtacggtt tttcgatgcc atagcgacgc ataactgtct
    13561 ggattggttc tgccagcact tcccagttgt gatccagctc atccagcaga tggtcacggt
    13621 tcacttccag tttgctcacg cctttcaggg tggattgata cgcaatcagc gcataaccga
    13681 tacccacgcc gaggttacgc agcacggtgg agtcggtcag gtcacgctgc cagcgggaaa
    13741 ccggcagttt gcttgccaga tgctgcaata ccgcgttgga aaggcccaga ttcccttcgg
    13801 agttttcgaa gtcgatcggg ttaactttat gcggcatggt ggaagaacca atctcaccag
    13861 caatggtttt ctgtttgaag tggttaaggg cgatataacc ccagacgtca cggtcaaagt
    13921 cgatcagaat ggtgttgaag cgcgcaacgc aatcaaacag ttcggcaatg tagtcgtgcg
    13981 gttcaatctg ggtagtgtac gggttccatt gaatacccag cgaggtgacg aactcttcgc
    14041 tgaactgatg ccagtcaact tccgggtaag cggcgatgtg ggcgttatag ttaccgaccg
    14101 cgccgttgat tttgccgagg atctccacct ggttaagctg gcggtgctgg cgctccatac
    14161 ggtaggcgac gtttgccatc tctttaccga tggttgacgg cgtggctggc tgaccgtggg
    14221 tacgagacag cagcgggata tcgcggtact gaacggcgag atctttaatg ccatcaatca
    14281 gttgacgcca gtacggcagg atcacttcat cacgcgcggt tttcagcatt aatgcgtggg
    14341 agaggttatt gatatcttcc gaagtacagg caaagtggat gaattcagaa accgcgtgca
    14401 gttccgggat ctccgccact ttttctttca ggaaatactc aaccgcttta acgtcgtggt
    14461 tagtggtacg ctcgatggtt ttgatgcgtg cggcatcttc ttcgctgaaa ttggcgacaa
    14521 ttgcatcaag gtaaccgatt gcgtcggcag caaaagcagg aacttccttg atcgctgcgt
    14581 gcgcggccag tttttgcagc caacgtactt caacttgtac acggaatttc agcaaaccat
    14641 attcgctgaa aatcccgcgc agcgcgctga ctttatcgcc gtagcgtcca tcgacagggg
    14701 aaacggcggt cagtgaggat aattccatag atcacaactc cggggttaaa tgagcaagaa
    14761 tttgttttgc ctgagtggtc aggcgattac gagaaaacat cagttgtaga cgtccgccgc
    14821 cgacctggtg ccagagcacg gcggcgcgaa tgcctgccag cagggttgcg cgaactttcg
    14881 cctgcacttg tgggctttgc agtacagcag gggaaccggt gacctgaatg cgcgggccaa
    14941 gcgggctgat cacatcaaca tagatagcag ccatcgcgct catcagcgtt tcggactgta
    15001 aatcgaagtg ttcgagctgg cgttgcaggc cgttgatccg gttgcccaga gtgtcgagcg
    15061 cgcctttcgc tgaggagagt ttgcgctcaa gcaccatcaa gctgagtgtg tagcgggtta
    15121 attcggcgtt taagccctgg caactgctgg cattgagcac gccgagcagg gtttccagcc
    15181 cgacgcgcag gttggcttcg ctaccgccaa aaaccgccag cgtcgagctg gggttcatat
    15241 caataatact gttgagtgag acgtgtagcg catcggcatc acaatgcccc tggtgagcga
    15301 gttgttgcac caggcgtgcc gactgacaaa taccggccag ggcgagggtg atgtcatagt
    15361 aattctttgc cacgttcact gcttccttgt tttaagtaaa gataataatc agaccggcag
    15421 cggcagacgc tgctcaataa taccgccacc gaggcacact tcaccgttat agaagacggc
    15481 agactggccc ggcgtcacgg cggcaaccgg ctcatcgaaa atcactttaa tgcgatcgtc
    15541 gtccagcgcc ttgacggtgc acggaatgtc ggtctggcga tagcgggttt ttaccgtgca
    15601 acgcatagtg ccggtgaatg gttcgcgatc gacccagtgc agctgctggg caatcaaccc
    15661 gacagacatc agccgcgggt gttcatggcc ctgagcgaca accagaatgt tgttttcgac
    15721 gtctttgtcc accacatacc acggttcttc ggtaccttct ttggtgccac cgatacccag
    15781 acctttacgc tgaccgagag tgtgatacat cagcccctgg tgctcaccaa tttcatcgcc
    15841 atcgacggta atgattttgc ccggttgcgc cgggagataa cggcccagga actcgcggaa
    15901 tttacgttcg ccaatgaagc aaatgccggt agagtctttt ttcttcgcgg tgaccagacc
    15961 aagatcttca gcaatcttac gcacctgcgg tttttccagt tcgcctaccg ggaacaggct
    16021 ttgcgcaatc tgctcatggc tgagcgtata aaggaagtag ctctggtctt tattgctgtc
    16081 cagaccacgc agcaggcggc tcttgccatc gacatcggcc cgacgtacgt aatgaccggt
    16141 agcgatataa tcggcaccta aatcttcggc ggcaaattcg aggaaggctt taaatttgat
    16201 ctccttgttg cacagaatat ccggattcgg cgtgcgaccg gctttatatt cggcaaggaa
    16261 cagttcgaag acgttgtccc agtactcggc agcaaagtta acggtgtgca gttcaatgcc
    16321 gagcttgtcg cagaccgcct gggcatcagc cagatccgct gctgctgtgc aatattcctc
    16381 accgtcgtct tcttcccagt tcttcataaa caggccttcg acctgatatc cctgttgttg
    16441 caacagccag gcagaaacgg aggaatcgac accgccggac atgccgacga ttactttttt
    16501 tgcggtttca gacattggat cactcacgac attgaacttc aaggcggcgt attctatcac
    16561 gcagcctttt agatgacacc ctttgtaaaa ggccagttaa aatcgccaat catctccagc
    16621 ggataacgtt gcccgctttg ataacaacga atactttccg ccaccagcgg cgaacgaaga
    16681 tttgacgcct gtaaaatttc ttcggcgctg acccaacggc agcagtcgat atcgctgtca
    16741 tgaggctggg tcgggcatat ttgctcaagc tcaatggcaa agaggaaacg caaaaacggc
    16801 gttttatctg gcgcaatcca ctgatgcata cgaataaagt gttgcggctg cgcgctgatg
    16861 ccggtttctt cccacagttc acgggcggcg gcttccacta aggtttcatc ggcttccaga
    16921 tgcccggcag gttggttcca taacgcttta ccattaatcg tctcttcaac gactaaaaat
    16981 ttgccttctg cgtgcaccac gcaagcaacg gtaacgtgcg gtttaaacat tttgcatcct
    17041 taatctgtca cttctcgcca ttcgccattg gcaagattat ccaaagagta atctcccatc
    17101 gcatagcgaa tcagtcgcag cgtggggaaa ccaacatggg cggtcatgcg gcgcacctgg
    17161 cgattacgtc cttcatataa ggtgatcttc agccagctgg tgggaatact tttgcgttca
    17221 cgaattggtg gattccgcgg ccataaccac gcgggttcgt caaccagctc cgcgcctgcg
    17281 ggcagggtag ggccatcatt taaggttacg ccattgcgca aggcttcaag tgcgtcttgt
    17341 gtgggaatac cttccacctg cacataatag atttttccgg tgcgtttacc cggctgggtt
    17401 aaacgcgcct gcaacgcgcc gttattggtc agcaccagca acccttcgct atcgcggtca
    17461 aggcgacctg ctgcataaac gccctgaacc gggatgaatt cttttaatgt tttgcgtccg
    17521 gtttcatcgg tgaactgcgg aagaacatcg tagggtttat tgaagaggat cacacgcgtg
    17581 ggctggtttt catgtttacg cctggtagaa cgttgcgagc tgaatcgctt aacctggtga
    17641 tttctaaaag aagttttttg catggtattt tcagagatta tgaattgccg cattatagcc
    17701 taataacgcg catctttcat gacggcaaac aatagggtag tattgacaag tcaattacaa
    17761 atcattaaca aaaaattgct ctaaagcatc cgtatcgcag gacgcaaacg catatgcaac
    17821 gtggtggcag acgagcaaac cagtagcgct cgaaggagag gtgaatggaa agtaaagtag
    17881 ttgttccggc acaaggcaag aagatcaccc tgcaaaacgg caaactcaac gttcctgaaa
    17941 atccgattat cccttacatt gaaggtgatg gaatcggtgt agatgtaacc ccagccatgc
    18001 tgaaagtggt cgacgctgca gtcgagaaag cctataaagg cgagcgtaaa atctcctgga
    18061 tggaaattta caccggtgaa aaatccacac aggtttatgg tcaggatgtc tggctgcctg
    18121 ctgaaaccct tgatctgatt cgtgaatatc gcgttgccat taaaggcccg ctgaccactc
    18181 cggttggtgg cggtattcgt tctctcaacg ttgccctgcg tcaggaactg gatctctaca
    18241 tctgcctgcg tccggtacgt tactatcagg gcactccaag cccggttaaa caccctgaac
    18301 tgaccgatat ggttatcttc cgtgaaaact cggaagacat ttatgcgggt atcgaatgga
    18361 aagctgactc tgccgacgcc gagaaagtga ttaaattcct gcgtgaagag atgggcgtga
    18421 agaaaattcg cttcccggaa cattgcggta tcggtattaa gccgtgttct gaagaaggca
    18481 ccaaacgtct ggttcgtgca gcgatcgaat acgcaattgc taacgatcgt gactctgtga
    18541 ccctggtgca caaaggcaac atcatgaagt tcaccgaagg cgcgtttaaa gactggggct
    18601 accagctggc gcgtgaagaa tttggcggtg aactgatcga cggcggcccg tggctgaaag
    18661 ttaaaaaccc gaataccggc aaagagatcg tcattaaaga cgtgattgct gatgcattcc
    18721 tgcaacagat cctgctgcgt ccggctgaat atgatgttat cgcctgtatg aacctgaacg
    18781 gtgactacat ttctgacgct ctggcagcgc aggttggcgg tatcggtatc gcccctggag
    18841 caaacatcgg tgacgaatgc gccctgtttg aagccaccca cggtactgcg ccgaaatacg
    18901 ccggtcagga caaagtaaac cctggctcta ttattctctc cgctgagatg atgttacgcc
    18961 atatgggctg gactgaagcc gcagacctga ttgttaaagg tatggaaggc gcgatcaacg
    19021 ccaagaccgt aacctatgac ttcgagcgtc tgatggaagg cgctaaactg ctgaaatgtt
    19081 cagagtttgg tgatgcgatc atcaagaata tgtaatcact acatgtgtta aatatttaaa
    19141 cgggcgtata acacgcccgt tgttttattt atgtggatat tattaatagc atatcgagca
    19201 t
//
LOCUS       DAZXUK010000051        19082 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZXUK010000051 DAZXUK010000000
VERSION     DAZXUK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19082
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.051"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..209)
                     /locus_tag="ACH3L8_001475"
     CDS             complement(<1..209)
                     /locus_tag="ACH3L8_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFT4084649.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACH3L8_001476"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACH3L8_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFT4084650.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACH3L8_001477"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACH3L8_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFT4084651.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACH3L8_001478"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACH3L8_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFT4084652.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACH3L8_001479"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACH3L8_001479"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFT4084653.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACH3L8_001480"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACH3L8_001480"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFT4084654.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACH3L8_001481"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACH3L8_001481"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFT4084655.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ACH3L8_001482"
     CDS             complement(4598..5320)
                     /locus_tag="ACH3L8_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFT4084656.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACH3L8_001483"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACH3L8_001483"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFT4084657.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="ACH3L8_001484"
     CDS             6520..6738
                     /locus_tag="ACH3L8_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFT4084658.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACH3L8_001485"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACH3L8_001485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFT4084659.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="ACH3L8_001486"
     CDS             complement(7472..7672)
                     /locus_tag="ACH3L8_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFT4084660.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACH3L8_001487"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACH3L8_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACH3L8_001488"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACH3L8_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFT4084661.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="ACH3L8_001489"
     CDS             10101..12014
                     /locus_tag="ACH3L8_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFT4084662.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="ACH3L8_001490"
     CDS             12014..13036
                     /locus_tag="ACH3L8_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFT4084663.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="ACH3L8_001491"
     CDS             13030..13248
                     /locus_tag="ACH3L8_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFT4084664.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="ACH3L8_001492"
     CDS             13302..14171
                     /locus_tag="ACH3L8_001492"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFT4084665.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="ACH3L8_001493"
     CDS             complement(14226..14630)
                     /locus_tag="ACH3L8_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFT4084666.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="ACH3L8_001494"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="ACH3L8_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFT4084667.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="ACH3L8_001495"
     CDS             15615..17705
                     /locus_tag="ACH3L8_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFT4084668.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACH3L8_001496"
                     /gene_synonym="dapC"
     CDS             complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACH3L8_001496"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFT4084669.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
BASE COUNT         4708 a         5185 c         4712 g         4477 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacgatctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa agggcgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggtttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 ga
//
LOCUS       DAZXUK010000052        18944 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZXUK010000052 DAZXUK010000000
VERSION     DAZXUK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18944
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.052"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..246
                     /locus_tag="ACH3L8_001497"
     CDS             <1..246
                     /locus_tag="ACH3L8_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFT4084670.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACH3L8_001498"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACH3L8_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFT4084671.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACH3L8_001499"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACH3L8_001499"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFT4084672.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="ACH3L8_001500"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="ACH3L8_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFT4084673.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACH3L8_001501"
     CDS             complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACH3L8_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFT4084674.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACH3L8_001502"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACH3L8_001502"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFT4084675.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="ACH3L8_001503"
     CDS             complement(4442..5650)
                     /locus_tag="ACH3L8_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4084676.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="ACH3L8_001504"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="ACH3L8_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFT4084677.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="ACH3L8_001505"
     CDS             complement(6445..8130)
                     /locus_tag="ACH3L8_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFT4084678.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="ACH3L8_001506"
     CDS             8400..8777
                     /locus_tag="ACH3L8_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFT4084679.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACH3L8_001507"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACH3L8_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFT4084680.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="ACH3L8_001508"
     CDS             9224..9511
                     /locus_tag="ACH3L8_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFT4084681.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9495..10217
                     /gene="nfsA"
                     /locus_tag="ACH3L8_001509"
     CDS             9495..10217
                     /gene="nfsA"
                     /locus_tag="ACH3L8_001509"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFT4084682.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10278..11180
                     /gene="rimK"
                     /locus_tag="ACH3L8_001510"
     CDS             10278..11180
                     /gene="rimK"
                     /locus_tag="ACH3L8_001510"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFT4084683.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11268..11744
                     /locus_tag="ACH3L8_001511"
     CDS             11268..11744
                     /locus_tag="ACH3L8_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFT4084684.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12096..13208
                     /gene="potF"
                     /locus_tag="ACH3L8_001512"
     CDS             12096..13208
                     /gene="potF"
                     /locus_tag="ACH3L8_001512"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFT4084685.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13303..14436
                     /gene="potG"
                     /locus_tag="ACH3L8_001513"
     CDS             13303..14436
                     /gene="potG"
                     /locus_tag="ACH3L8_001513"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFT4084686.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14446..15399
                     /gene="potH"
                     /locus_tag="ACH3L8_001514"
     CDS             14446..15399
                     /gene="potH"
                     /locus_tag="ACH3L8_001514"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFT4084687.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15396..16241
                     /gene="potI"
                     /locus_tag="ACH3L8_001515"
     CDS             15396..16241
                     /gene="potI"
                     /locus_tag="ACH3L8_001515"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFT4084688.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16301..16789
                     /locus_tag="ACH3L8_001516"
     CDS             16301..16789
                     /locus_tag="ACH3L8_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFT4084689.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16830..17957
                     /gene="rlmC"
                     /locus_tag="ACH3L8_001517"
     CDS             16830..17957
                     /gene="rlmC"
                     /locus_tag="ACH3L8_001517"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFT4084690.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="ACH3L8_001518"
     CDS             complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="ACH3L8_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFT4084691.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
BASE COUNT         4585 a         4666 c         4951 g         4742 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccccagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atgctaattt acgataatgt gttttaccag aattcagggc gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggcaaaa cgtcttgccc cacagctgat gaactgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc tccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata
     9901 ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt
     9961 gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg
    10021 tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac
    10081 tggcggaata ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata
    10141 tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg
    10201 gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc
    10261 tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt
    10321 gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc
    10381 tttcttgcta catgaacata aatcctgcgg cgtcttctat ttactacaaa ggccgcaagt
    10441 taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg
    10501 cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg
    10561 cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc
    10621 ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg
    10681 gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg
    10741 agactcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc
    10801 tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg
    10861 atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc
    10921 atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa
    10981 aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg
    11041 ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg
    11101 gtatcgatat cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt
    11161 gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt
    11221 tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg
    11281 tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg
    11341 aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg
    11401 atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac
    11461 cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg
    11521 tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt
    11581 tatccgttat gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag
    11641 agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg
    11701 atgaagggca aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca
    11761 cgcagtcgct gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct
    11821 taaagattat ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat
    11881 aaaaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt
    11941 attcttgcga tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta
    12001 caccgcgccg ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg
    12061 ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc
    12121 tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac
    12181 aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt
    12241 ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg
    12301 aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc
    12361 tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt
    12421 ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat
    12481 ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag
    12541 ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc
    12601 tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta
    12661 ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac
    12721 cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat
    12781 acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct
    12841 ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc
    12901 caaaagaagg ggcgatggcg ttctttgatg tattcgccat gccagcggat gccaaaaaca
    12961 aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt
    13021 ccgaccatgt gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag
    13081 tccgtgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga
    13141 aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg
    13201 gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac
    13261 catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct
    13321 cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc
    13381 aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa
    13441 atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctgcg tatgctggca
    13501 ggtttcgaac aaccttctgc cggacagata atgcttgatg gcgtcgattt gtcacaggtt
    13561 ccgccttacc tgcgccccat caatatgatg tttcagtctt acgcgctgtt tccgcatatg
    13621 accgtggaac agaacatcgc ttttggcctg aaacaggaca aactaccgaa agcggaaatt
    13681 tccagtcggg tcaatgagat gctcggcctg gtgcacatgc aggagttcgc caaacgtaaa
    13741 ccgcatcagc tttctggtgg tcagcgacag cgtgtggccc tggcccgaag ccttgccaag
    13801 cgcccgaaac tattactgct cgacgagccg atgggcgcgc tggataaaaa gctgcgtgac
    13861 cggatgcagc ttgaagtggt ggatattctg gagcgcgtcg gtgtgacttg tgtgatggtc
    13921 acccacgatc aggaagaggc gatgaccatg gcggggcgca tcgccattat gaatcgtggg
    13981 aaatttgtcc agattggcga accggaagag atctacgagc atccgactac ccgctatagc
    14041 gccgaattta ttggctcggt aaacgtcttt gaaggcgtac tcaaagagcg tcaggaagat
    14101 ggcttggtgc ttgattcgcc ggggctggtg catccactga aagtcgacgc ggatgcctcg
    14161 gtggtcgata acgtgccggt acatgtggcg ctgcgcccgg aaaaaatcat gctttgcgaa
    14221 gagccgcccg ccaatggttg taacttcgcg gtgggggagg tgatacacat tgcctatctc
    14281 ggcgatcttt cggtgtatca cgttcgtctg aaaagtgggc agatgattag tgcccagcta
    14341 caaaacgccc atcgtcatcg taaagggtta ccgacctggg gcgacgaagt gcgtttgtgc
    14401 tgggaagtgg acagctgtgt ggtgctgacg gtttaaggag caaagatgaa tacacttgaa
    14461 cctgctgccc agtcgaaacc gccgggcgga tttaagctgt ggttgtcgca gctgcaaatg
    14521 aagcatgggc gcaaactggt cattgcgttg ccatatatct ggttgatctt gctgtttctg
    14581 ctgccatttc tgattgtctt taaaataagc ctggcggaga tggcgcgcgc tattccacct
    14641 tataccgaac tgatggagtg ggctgacggg caactgtcca tcactcttaa tctcggtaat
    14701 tttctgcaac tgaccgacga tccgctctat ttcgatgctt atctccagtc gctacaggtg
    14761 gctgcgattt cgacattttg ctgtttactg atcggctatc cgctggcgtg ggcggtggcg
    14821 cacagtaagc cttcgacccg taatatttta ttactactgg tgatcctgcc gtcgtggacc
    14881 tcgtttctga tccgcgttta tgcttggatg ggaatattaa aaaacaacgg tgtgctgaat
    14941 aattttctgc tgtggctggg ggttatcgat caaccgctga ccattcttca taccaatctg
    15001 gcagtttata ttggcattgt ttacgcctac gtgccgttta tggtgctgcc gatttatacc
    15061 gcgttgattc gtattgatta ttcgctggtg gaagcagcgc tggatctcgg tgcacgaccg
    15121 ctgaaaacgt tctttactgt gatcgtgccg ctgactaaag gtgggattat tgccggatcg
    15181 atgctggtgt ttatcccggc tgtgggcgag tttgtgatcc cggaactgct cggtggcccg
    15241 gacagcatca tgatcgggcg cgtgctatgg caagagtttt tcaataaccg cgactggccg
    15301 gtggcctcgg cggtagcgat catcatgttg ctgctgctaa ttgtgccgat aatgtggttc
    15361 cacaaacacc agcaaaaaag cgtgggagaa cacggatgaa taatttaccg gtagttcgtt
    15421 cgccctggcg gattgtgatt ttgctgctgg gctttacctt tctctacgcg ccaatgctga
    15481 tgctggttat ctattcgttt aacagctcga agctggtgac ggtgtggggc ggctggtcaa
    15541 cgcgctggta tggtgagtta ttgcgcgatg acgcgatgat gagtgcggtt ggttcaagcc
    15601 tgacaattgc tgcctgtgcg gcaacggcgg cggcgatcct cgggactatt gcggcggtgg
    15661 tgctggtgcg ctttggcagg tttcgcggat caaatggctt tgcctttatg atcaccgcgc
    15721 cgctagtaat gccagatgtc atcacgggct tgtcgctgtt gttattattc gtcgcgcttg
    15781 ctcatgccat tggctggcct gcggaccgcg gtatgcttac catctggctg gcgcatgtca
    15841 ctttctgtac ggcttatgtg gcggtcgtta tttcgtcgcg tctgcgggaa ctggatcgct
    15901 cgatagaaga agccgcgatg gatctcggtg cgacgccgct gaaagtgttt ttcgtcatta
    15961 cgctaccgat gatcatgccc gcgatcattt ctggctggtt actggctttt actttgtcgc
    16021 ttgatgatct ggtgatcgcc agctttgttt ccgggccggg agccaccacg ttaccgatgc
    16081 tggtcttttc cagcgtgcgg atgggggtga atccggaaat caacgccctg gcaacattga
    16141 tcctcggcgc ggtcggaatt gtcggattta tcgcctggta tctgatggcg cgcgcagaaa
    16201 aacagcggat acgcgacatc caacgtgcaa gacgtggctg aagacactaa aatttgccaa
    16261 cctggctaca taatgccgcg catgtcgcgg cattgttttc atggaagacg aaacgttggg
    16321 attttttaag aaaacatctt catctcatgc tcgcctgaat gtgcctgcgc tggtgcaggt
    16381 ggcggcgctc gccattatta tgatccgtgg cctcgacgtg ctgatgattt tcaatacgct
    16441 gggcgtgcgc ggtattggcg agttcattca tcgcagcgta caaacctgga gtttaacgct
    16501 ggtcttttta agcagtctgg tgctggtttt cattgagatc tggtgtgcgt tttcactggt
    16561 gaaagggcgt cgctgggcgc gctggctata tctgctgaca caaatcaccg ccgcaagtta
    16621 cttgtgggcg gcttcgctgg ggtacggtta tccggagctg ttcagcattc ccggtgaatc
    16681 aaaacgtgaa atcttccata gcctgatgct gcagaagctg ccggatatgc tcatcctgat
    16741 gctgctgttc gttccctcga ccagtcggcg gttcttccag ttgcaataat gtgtataatc
    16801 gtcgcccctg atgatgtgaa ggtcaatgta tgcagtgcgc actttacgac gcgggtcgct
    16861 gtcgttcctg tcagtggata acgcagccga ttccagagca actctccgct aaaaccgccg
    16921 atcttaaaaa tctgctcgcc gattttccgg ttgaggaatg gtgcgcgccg gtgtcaggcc
    16981 cggaacaagg gtttcgtaat aaagccaaaa tggtggtgag tggtagcgtt gaaaaaacac
    17041 tgctcggtat gctgcatcgc gatggcacgc cggaagacct ttgtgactgc ccgctttatc
    17101 cagcctcatt tgcgcccgtt tttgcggcgc taaaaccgtt tatcgcccga gcggggttaa
    17161 cgccctacaa cgtggcgcgt aagcgtggcg aactgaaata cattctgctg actgaaagcc
    17221 agagtgatgg cggcatgatg ctgcgctttg tactgcgttc tgataccaag ctggcgcaac
    17281 tgcgtaaggc gctgccgtgg ttacacgaac aactaccgca gctgaaagtt attaccgtca
    17341 atattcagcc ggtacatatg gcgattatgg aaggggagac ggagatctac ctgaccgaac
    17401 aacaggcact ggcggagcgt tttaatgacg taccgctgtg gatccgtccg caaagtttct
    17461 tccagactaa tccggcggtc gccagccagt tgtacgccac cgcgcgcgac tgggtacgac
    17521 agctgccggt taaacatatg tgggatctgt tctgcggtgt ggggggcttt ggtttacact
    17581 gcgcgacgcc tgacatgcag ttaaccggga tcgaaattgc gccagaggcc attgcctgtg
    17641 caaagcagtc agccgctgaa ctgggcttaa ctcgtttaca atttcaggcg ctggactcca
    17701 ctcagtttgc cactgctcag ggggaggtgc cggagctggt gctggttaac ccgccgcgcc
    17761 gcggcattgg taaaccgctg tgtgattatc tttcaacgat ggcaccgcgt tttattatct
    17821 actccagctg taacgcccaa accatggcga aagatatccg cgaactgcca ggttaccgca
    17881 tcgaacgggt acagcttttt gatatgttcc cgcacaccgc gcactatgaa gtgttgacgc
    17941 tgctggtgaa gcaataaaaa agccacaggt gcggcttcag attgctgatc aggtgcgctt
    18001 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaatcatg ctaaatcaat
    18061 acattgcaga gttgatgtgg gccggataag cgcagcgcat caggcaatct tacttttgtc
    18121 atcagtctca attactgcgg aaaccactgg tcattgattt tctgatatgt accgtcagct
    18181 ttaattgctg ccagcgcgtt attcagtttt tccagcagag ctttgttatc cggacgtaca
    18241 gcgataccca ggccggtgcc gaagtattgc ggatcggtca ctttttcagt cgcaacgccc
    18301 agttgcggat tggttttcag ccattcgttt accaccgcgg tgtcaccaaa taccccatca
    18361 atacgaccat ttttcagatc gataaaggca ttctgataac tgtcatagga gacagttttc
    18421 acttccgggt gctgatcctg aatatatttc tggtgcgtcg taccgttttc catcccaatg
    18481 cgtttgcctt tcaggtcggc aaacgttttg taggtatctt ttttggaaat cacgacggct
    18541 gagttttcat agtagggtgt ggtaaacgaa acctgcttgc tacgctccgg ggtgatatcc
    18601 ataccggaga ttacggcgtc atattttctg aatttcagtg acgggatcag gctgtcgaac
    18661 gcgtgattag taaaagtaca ttctgcctgc atttgtttgc acaaggcttt tgccagatcg
    18721 atatcaaagc cgacaatctc attattagca cctatagatt caaagggtgg ataggtggct
    18781 gaaacgccaa aattgatttt ctctgcggca gaagcaccga aagtaaagga agcaagtaaa
    18841 gcggcaagaa ctaacttttt catgatggaa ctcccgtctg tcaatcttat gatttttggc
    18901 cgtgtctgcg gcatgggata acaatgccat caagtgaatt tata
//
LOCUS       DAZXUK010000053        18894 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZXUK010000053 DAZXUK010000000
VERSION     DAZXUK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18894
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.053"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1025)
                     /locus_tag="ACH3L8_001519"
     CDS             complement(<1..1025)
                     /locus_tag="ACH3L8_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4084692.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="ACH3L8_001520"
     CDS             complement(1127..2107)
                     /locus_tag="ACH3L8_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFT4084693.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="ACH3L8_001521"
     CDS             complement(2115..2825)
                     /locus_tag="ACH3L8_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFT4084694.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACH3L8_001522"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACH3L8_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFT4084695.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="ACH3L8_001523"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="ACH3L8_001523"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFT4084696.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="ACH3L8_001524"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="ACH3L8_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFT4084697.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="ACH3L8_001525"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="ACH3L8_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFT4084698.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="ACH3L8_001526"
     CDS             9402..9512
                     /locus_tag="ACH3L8_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFT4084699.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACH3L8_001527"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACH3L8_001527"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFT4084700.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACH3L8_001528"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACH3L8_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFT4084701.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(11931..12097)
                     /locus_tag="ACH3L8_001529"
                     /pseudo
     CDS             complement(11931..12097)
                     /locus_tag="ACH3L8_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12394..13026
                     /gene="yihA"
                     /locus_tag="ACH3L8_001530"
     CDS             12394..13026
                     /gene="yihA"
                     /locus_tag="ACH3L8_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFT4084702.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACH3L8_001531"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACH3L8_001531"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFT4084703.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16558..17490
                     /locus_tag="ACH3L8_001532"
     CDS             16558..17490
                     /locus_tag="ACH3L8_001532"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFT4084704.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17531..>18894)
                     /locus_tag="ACH3L8_001533"
     CDS             complement(17531..>18894)
                     /locus_tag="ACH3L8_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFT4084705.1"
                     /translation="TGTQIQPGIEKFIKDFNDAKKKGEHAYDMTLSYKNFDKGFFNSR
                     FQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPITMLMHGNVIPALAAAKVNLVNNE
                     LTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAPAEYGKFSFGEGQFTFNGDGSSLS
                     NLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLEEKKFPVGESESKFNQINIINHGE
                     DVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLTKGNQQLGSGEWSLIAESIDPSAV
                     RQFIIQYNIAMQKQLAAHPELANDEVALQEVNAALFKEYLPLLQKSEPTIKQPVRWKN
                     ALGELNANLDISIADPAKSSSSTNKDIKSLNFDVKLPLNVVTETAKQLNLSEGMDAEK
                     AQKQADKQISGMMTLGQMFQLITIDNNTASLQLRYTPGKVVFNGQEMSEEEFMSRAGR
                     FVH"
BASE COUNT         4603 a         4769 c         4540 g         4982 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcacttgcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacgctcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg cggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    16381 catgatgttt gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga
    16441 taatgggatt ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg
    16501 ttatttgggt cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg
    16561 gctaaccttt tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg
    16621 agtatcgtat tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg
    16681 attgtaaaac tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat
    16741 tttatgatgt attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg
    16801 caatgggaag ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt
    16861 aaccaccgta gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg
    16921 atgaataaat atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc
    16981 tgggcgctgg atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg
    17041 gagcgacgtg gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat
    17101 cccaccacca ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa
    17161 acccactcca cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta
    17221 aatgtactgg gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat
    17281 aaccgtcagc cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg
    17341 gatttacagc ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag
    17401 cgccattttc agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct
    17461 ctcatgtcat cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg
    17521 agtgagtatt ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct
    17581 cctgtccgtt aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt
    17641 tgtcaatcgt gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat
    17701 cagcctgctt ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg
    17761 tttctgtgac gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt
    17821 ttgtggatga tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt
    17881 cgccgagtgc gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata
    17941 acggtaaata ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg
    18001 ctaactcagg gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt
    18061 ggcgcactgc tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt
    18121 gctgattccc ttttgttaac ttatcaagtt cgtaggtcag attgacattg atataatctt
    18181 tgtctttaac gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt
    18241 ccccgtgatt gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt
    18301 tcttttcttc taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca
    18361 ttggtgataa ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg
    18421 aactaccatc accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg
    18481 caggggcaac atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt
    18541 ccacgggcga tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta
    18601 agttcacttt tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg
    18661 gcaacggacc gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga
    18721 gatcgggtgc accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt
    18781 tgtcaaagtt tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat
    18841 cgttaaaatc tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgt
//
LOCUS       DAZXUK010000054        18388 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZXUK010000054 DAZXUK010000000
VERSION     DAZXUK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18388
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.054"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..677
                     /gene="bcsQ"
                     /locus_tag="ACH3L8_001534"
     CDS             <1..677
                     /gene="bcsQ"
                     /locus_tag="ACH3L8_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFT4084706.1"
                     /translation="QMLGENVLVVDACPDNLLRLSFNVDFTHRQGWARAMLDGQDWRD
                     AGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGRYQWILIDLPRD
                     ASQITHQLLSLCEHSLAIVNVDANCHIRLHQQALPDGAHILINDFRIGSQVQDDIYQL
                     WLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANWCLLNYSGLKT
                     PVGSAS"
     gene            674..3291
                     /gene="bcsA"
                     /locus_tag="ACH3L8_001535"
                     /pseudo
     CDS             674..3291
                     /gene="bcsA"
                     /locus_tag="ACH3L8_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3302..5641
                     /gene="bcsB"
                     /locus_tag="ACH3L8_001536"
     CDS             3302..5641
                     /gene="bcsB"
                     /locus_tag="ACH3L8_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFT4084707.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            5648..6754
                     /gene="bcsZ"
                     /locus_tag="ACH3L8_001537"
     CDS             5648..6754
                     /gene="bcsZ"
                     /locus_tag="ACH3L8_001537"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFT4084708.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            6949..7338
                     /locus_tag="ACH3L8_001538"
     CDS             6949..7338
                     /locus_tag="ACH3L8_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HFT4084709.1"
                     /translation="MISNAACKTTVWHSRQRYWKTRANGRRRQHFSGNDWRWAPAAYG
                     LLTDWQEEAARQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQVKDYAPSHFL
                     LYVRYSAAGWQGDMDLPPQPLIPYADW"
     gene            7420..9408
                     /gene="hmsP"
                     /locus_tag="ACH3L8_001539"
     CDS             7420..9408
                     /gene="hmsP"
                     /locus_tag="ACH3L8_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFT4084710.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            9595..10881
                     /gene="dctA"
                     /locus_tag="ACH3L8_001540"
     CDS             9595..10881
                     /gene="dctA"
                     /locus_tag="ACH3L8_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFT4084711.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            11102..12598
                     /locus_tag="ACH3L8_001541"
     CDS             11102..12598
                     /locus_tag="ACH3L8_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFT4084712.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(12694..13623)
                     /gene="kdgK"
                     /locus_tag="ACH3L8_001542"
     CDS             complement(12694..13623)
                     /gene="kdgK"
                     /locus_tag="ACH3L8_001542"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFT4084713.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            13855..14622
                     /gene="pdeH"
                     /locus_tag="ACH3L8_001543"
     CDS             13855..14622
                     /gene="pdeH"
                     /locus_tag="ACH3L8_001543"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFT4084714.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            14692..16752
                     /locus_tag="ACH3L8_001544"
     CDS             14692..16752
                     /locus_tag="ACH3L8_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFT4084715.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(16934..18256)
                     /locus_tag="ACH3L8_001545"
     CDS             complement(16934..18256)
                     /locus_tag="ACH3L8_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4084716.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
BASE COUNT         4233 a         4706 c         5132 g         4317 t
ORIGIN      
        1 tacaaatgtt gggagaaaat gtcctggtgg tcgatgcctg cccggacaac ttgttgcgcc
       61 tgtcatttaa tgttgatttt acccaccgtc agggctgggc cagagcgatg ctggatggcc
      121 aggactggcg tgacgctggg ttgcgctaca cctcgcagct cgatttgctg ccttttggtc
      181 agttatccat tgaagaacaa gaaaatccac agcactggca aacccggctg agcgatattt
      241 gctccggctt acagcaacta aaagccagcg ggcgttacca gtggatttta atcgacttac
      301 cgcgtgatgc ctcgcagata acccaccagc tgctgagttt gtgcgagcac tcgctggcaa
      361 tcgtcaatgt ggatgccaac tgccatatcc gactgcatca gcaagcgctg ccggatggcg
      421 cacatatttt gattaatgac ttccgtattg gcagtcaggt tcaggacgat atttaccagc
      481 tttggttgca aagccagcgc cgattactgc cgatgctcat tcatcgtgat gaagcgatgg
      541 ctgaatgcct ggcggctaag caaccagtag gtgaatatcg cagtgatgcg ctggcggctg
      601 aagagatact gacgctggcg aactggtgcc tgttgaacta ctccgggctg aaaacgccag
      661 tcgggagtgc atcatgagta tcctgacccg gtggttgctt atcccgccgg tcaacgcgcg
      721 gcttatcggg cgttatcgcg attatcgtcg tcacggtgcg tcggctttca gcgcgacgct
      781 cggctgtttc tggatgatcc tggcctggat ttttattccg ctggagcacc cgcgctggca
      841 gcgtattcgc gcagaacata aaaacctgta tacgcatatc aacgcctcgc gtccgcgtcc
      901 gctggacccg gtccgttatc tcattcaaac atgctggtta ttgatcggtg catcgcgcaa
      961 agaaacgccg aaaccgcgca gcgggcattt tcaggtctgc aaaatattcg tggacgttac
     1021 catcaatgga tgaacgagct gcctgagcgc gttagccata aaacacagca tctggatgaa
     1081 aaaaaagagc tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc
     1141 accttctcgc tgattctggc gttaatctgc gttactcagc cgtttaaccc gctggcgcag
     1201 tttatcttcc tgatgctgct gtggggggta gcgctgatcg tacggcggat gccggggcgc
     1261 ttctcggcgc taatgttgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg
     1321 cgttacacct ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg
     1381 ctcttcgctg aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtagtatgg
     1441 ccgctgaatc gtcagccggt gccattgccg aaggatatgt cgctgtggcc gtcagtggat
     1501 atctttgtcc cgacttacaa cgaagatctc aatgtggtga aaaataccat ttacgcctcg
     1561 ctgggtatcg actggccgaa agacaaactg aacatctgga tcctcgatga cggcggcagg
     1621 gaagagtttc gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg caccactcat
     1681 gaacatgcga aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg
     1741 tcgattttcg actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg
     1801 ttcctgaaag agaaacagct ggcgatgatg cagactccac accacttctt ctcgccggac
     1861 ccgtttgaac gcaacctggg acgttttcgt aaaacgccga acgaaggcac gctgttctat
     1921 ggtctggtgc aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctgtgcg
     1981 gtgattcgtc gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac cgtgactgaa
     2041 gatgcgcata cttctctgcg tttgcaccgt cgtggctata cctccgcgta tatgcgtatt
     2101 ccgcaggcgg cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc
     2161 tgggcgcgcg ggatggtata aatcttccgt ctcgataacc cgctcaccgg taaaggactg
     2221 aagtttgctc agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca
     2281 cggctgatct tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat
     2341 gcgccagcgt tgatgatcgc cctattcgtg ctgccgcata tgatccatgc cagcctgacc
     2401 aactcgaaga tccagggcaa atatcgccac tctttctgga gtgaaatcta cgaaacggtg
     2461 ctggcgtggt atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa
     2521 tttaacgtca ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg
     2581 cggccctaca tcttccttgt tctactcaac ctggtgggcg ttgcggtagg catctggcgc
     2641 tacttctatg gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtgttc
     2701 tacaacctga ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc
     2761 cgatcgaacc gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc
     2821 ttctcgtgta ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag
     2881 gcgcagattc tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac
     2941 gtcttcccga cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg
     3001 ctcaccaccc agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg
     3061 gcgctctggc aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg
     3121 aagctcggct tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc
     3181 atattccgtg tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccgcccg
     3241 gagcggagcg aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc
     3301 gatgaaaaga aaactattct ggatttgtgc agtggctatg gggatgagtg cgttcccctc
     3361 tttcatgacg caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc
     3421 cgcccagacg gaacaaaatc cgcaggtggg gcaagtgatg ccaggcgagc agggagctga
     3481 tgcccccatc gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat
     3541 cgcgccgccg ccgggcagca tggtgctacg tggtattaac ccgaacggca gcattgagtt
     3601 tggtatgcgc agcgatgaag tggtgacgaa ggcgatgctc aacttcgaat acacgccatc
     3661 gccatcgtta ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg
     3721 cgtgctgcca gtgaccaaag aacagttggg taaaaaaacg ctggcgcaaa tgcccattaa
     3781 tccactgttt attaccgact tcaaccgtgt gcggctggag tttgtcggcc attatcagga
     3841 cgtgtgcgaa aacccggcca gcaccacgct ttggctggat gttgggcgga gcagtggact
     3901 ggatctgacc tatcagaccc tgaatgtgaa gaatgacctg tcacacttcc cggtgccatt
     3961 ctttgacccg cgcgataacc gtactaacac cttgccgatg gtctttgcgg gtgcgccgga
     4021 tgttgggctg caacaagcat ctgccattgt cgcctcgtgg tttggttcgc gttctggctg
     4081 gcgtgggcag aacttcccgg tgctctataa ccaattgccg gatcgcaatg ccattgtctt
     4141 tgcaaccaac gacaaacggc cggacttcct gcgcgatcat ccggcggtaa aagccccggt
     4201 gattgagatg attaaccatc cgcagaatcc ttacgtcaaa ctgctggtgg tgtttggtcg
     4261 tgacgacaaa gacctgttgc aggcagcgaa aggtatcgct cagggtaaca ttctgttccg
     4321 tggtgaaagc gtggtagtga atgaagtgaa accgctgcta ccgcgtaagc cgtacgatgc
     4381 gccgaactgg gtacgtaccg atcgtccggt cacatttggc gaactgaaaa cctatgaaga
     4441 acagttacaa tccagcggcc ttgagccagc agcgattaac gtttcgctaa acctgccgcc
     4501 ggatctctac ctgatgcgca gtaccggcat tgatatggat attaattacc gctacaccat
     4561 gccgccggtg aaagacagtt cgcggatgga tatcagcctg aataaccagt tcctgcaatc
     4621 cttcaacctg agcagcaaac aggaggcgaa ccgtctgctg ctgcgtattc cggtattaca
     4681 aggtttgctg gatggcaaaa cagatgtctc tattccggcg ctgaaactgg gcgcgaccaa
     4741 ccagctgcgc ttcgactttg agtatatgaa cccgatgcca ggcggttcgg tagataactg
     4801 tattaccttc cagccggtgc agaatcatgt ggtgattggt gacgattcca ccatcgactt
     4861 ctcgaagtat taccacttca tcccgatgcc ggatctacgc gcctttgcta acgcgggctt
     4921 cccattcagc cggatggcgg atctgtcgca aaccatcacc gtgatgccga aagcgcctaa
     4981 cgaagcacag atggaaacgt tgctgaatac tgttggtttt atcggcgcac agacgggctt
     5041 cccggcgatt aatctgacgg tgaccgatga tggcagcacc attcagggca aagatgccga
     5101 catcatgatc atcggtggta tcccggacaa actgaaagac gataagcaga tcgacctatt
     5161 ggtgcaggcg accgaaagct gggtgaaaac accgatgcgc cagaccccgt tccccggcat
     5221 tgtgccggac gagagcgatc gcgcggcaga aacccagtca acgctgacct cttccggtgc
     5281 gatggcggcg gtgattggct tccagtcgcc gtataacgac cagcgcagcg tgattgcgct
     5341 gttggcagat agcccacgcg gttatgaaat gcttaacgat gcggtgaacg atagcggcaa
     5401 acgcgccacc atgttcggtt cggtcgcggt gatccgcgag tccggtatca acagcctacg
     5461 tgttggcgac gtttattacg taggtcatct gccgtggttc gagcgcttgt ggtatgcgct
     5521 ggcaaaccat ccgattctgc tggcggtgct ggcgactatc agtgtgatat tgctggcatg
     5581 ggtactgtgg cgtctgctgc gaattattag tcgtcgtcgt cttaacccgg ataacgagta
     5641 attgaagatg aatgtgttgc gtagtggaat cgtgacgatg ctgttgttgg ctgcctttag
     5701 tgttcaggca gcctgtacct ggcctgcctg ggagcagttt aaaaaggatt acatcagtca
     5761 ggaagggcgc gtcatcgacc ccagcgacgc gcgcaaaatc accacctccg aagggcaaag
     5821 ttacggtatg ttctttgccc tggcggctaa cgaccgtgca gctttcgata atattctcga
     5881 ctggacgcag aacaatctcg ctcagggttc tttaaaagaa cgtttgcccg cctggctgtg
     5941 gggcaagaaa gagaacagta agtgggaagt gctggacagc aattcggcct ccgatggtga
     6001 tgtctggatg gcctggtcgt tgctggaggc ggggcgtttg tggaaagagc agcgttatac
     6061 cgacatcggc agcgcgttgc taaaacgtat cgcgcgggag gaagtggtga cggtgcctgg
     6121 gctgggttcc atgttgttac cgggcaaagt gggttttgct gaggataaca gctggcgttt
     6181 taaccccagc tacatgccgc cgacgctggc gcagtatttc acccgctttg gcgcgccgtg
     6241 gaccacgctg cgcgaaacca atcaacattt attgctggaa accgccccga aaggcttttc
     6301 gccagactgg gtgcgctatg agaaagacaa aggctggcag ctaaaagccg aaaaaacatt
     6361 gatcagcagc tacgacgcta tccgcgttta catgtgggta ggcatgatgc ctgacagcga
     6421 tccgcaaaaa gcgcggatgc tcaaccggtt taaaccgatg gcgacattca ctgagaaaaa
     6481 cggttatccg ccggaaaaag tggatgtggc tacggggaaa gcgcagggta aaggaccggt
     6541 cggtttttct gccgccatgc tgcctttttt acaaaaccgc gatgcgcagg cagttcagcg
     6601 ccagcgcgtg gccgataact ttcccggcag cgatacctat tacaactatg tgctgaccct
     6661 gtttggacaa ggctgggatc aacaccgttt ccgcttctcg acaaaaggtg agttattacc
     6721 tgactggggc caggaatgcg caaattcaca ctaaacatat tcacgctttc cctcggtctg
     6781 gccgtcatgc cgatggtcga ggcagcacca accatcagca gaccctgcgg atggacagcg
     6841 gcaacactaa tgccgtgcgc gggctggcga atatttaccg ccagcagtcg ccagaaaaag
     6901 ctgaagcgtt tatcgcctcg ctctctgcca gccagcggcg cagcattgat gatatcgaac
     6961 gcagcctgca aaacgaccgt ctggcacagc aggcagaggt actggaaaac cagggcaaat
     7021 gggcgcaggc ggcagcactt cagcggcaac gactggcgct gggccccggc agcgtatgga
     7081 ttacttaccg actggcagga agaagctgcg cggcaatcca acgatggcgg cagcagtcag
     7141 ggctttggct acacggcgcg ggcattactt gaacgacgtg ttacttccaa ctggtttgtt
     7201 ggcacggcaa ttgatatcca gcaggtgaaa gattacgcac ccagccattt cctgctctac
     7261 gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca gccgctgata
     7321 ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg cgatcgggta
     7381 tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag tcgctcgtta
     7441 acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt tattttttgc
     7501 accgttttgc tgttccatct ggtccagcag aatcgctata acacggctac gcaactggaa
     7561 agcattgctc gctctgtccg cgaaccctta tcttcagcta ttttgaaagg cgatattccc
     7621 gaagcggaag ctattcttgc cagcattaaa ccggcaggcg tggtcagccg tgccgatgta
     7681 gtgctgccta accagttcca ggcgctgcgt aaaagtttta ttccagagcg tccggtgccg
     7741 gtaatggtta ctcgcctgtt tgagctaccg gttcaaatct cgctgggcgt ctactcgctc
     7801 gaacgtccgg caaatccgca gccaatagcc tatctggtgc tacaggcgga ttccttccgt
     7861 atgtataagt tcgtgatgag cactctctca acgttagtga ccatttactt acttttgtcg
     7921 cttatcctga ccgtcgccat cagctggtgc attaaccgcc tgattttgca tccgttacgc
     7981 aatattgctc gcgaacttaa cgccatccca gcccaggagc ttgttggtca ccaactggca
     8041 ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta caacctcaac
     8101 cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg cttcccggtg
     8161 tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggttgt cgcgcgtaaa
     8221 caaaccaccg cgctgatgat catcacctgt gaaaccctgc gtgatactgc gggcgtgctg
     8281 aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa atcggtactg
     8341 tcgccacgta tgatcctcgc gcagattagc ggttatgact ttgctgtcat tgccaacggt
     8401 gtacaggaac cgtggcacgc aatcactttg ggtcagcaag tactcactat catgagcgag
     8461 cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattggcgt ggcgatgttc
     8521 tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc atttaccgct
     8581 cgccataaag gcaagaatca gattcagttc tttgatccgc agcagatgga agcagcccag
     8641 aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca gtttgcaatt
     8701 tggttacagc cacaggtcga gatgaccagc ggtaaactgg tcagtgcgga agtgttactg
     8761 cgtatccagc aaccggatgg cagttgggac ctgccggatg gcttaatcga tcgcattgag
     8821 tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg tcgattgctt
     8881 gcagcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc tgcgctgcaa
     8941 ctgatgcacc cgaatatggt ggcggatatg ctggaactgt taacccgcta tcgcattcag
     9001 ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgaccc tcatgctgcg
     9061 gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga tgatttcggc
     9121 atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat cgacgtactg
     9181 aaaatcgaca aaatgtttgt tgaaggcttg ccggaagata gcagcatgat tgctgcaatt
     9241 atcatgctgg cgcagagcct gaacttacaa atgattgccg aaggcgtgga gactgaagca
     9301 caacgagact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct ttttgctcgc
     9361 ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct accccaaact
     9421 gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt ttaacctttt
     9481 tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat tttaaggccg
     9541 caggtacccc ccccataacc ttacaagacc tgtggtttta ctaaaggaca ccctatgaaa
     9601 acctctctgt ttaaaagcct ttactttcag gtcctgacag cgatagccat tggtattctc
     9661 cttggccatt tctatcctga aataggcgag caaatgaaac cgcttggcga cggcttcgtt
     9721 aagctcatta agatgatcat cgctcctgtc atcttttgta ccgtcgtaac gggcattgcg
     9781 ggcatggaaa gcatgaaggc ggtcggtcgt accggcgcag tcgcactgct ttactttgaa
     9841 attgtcagta ccatcgcgct gattattggt cttatcatcg ttaacgtcgt gcagcctggt
     9901 gccggaatga acgtcgatcc ggcaacgctt gatgcgaaag cggtagcggt ttacgccgat
     9961 caggcgaaag accagggcat tgtcgccttc attatggatg tcatcccggc gagcgtcatt
    10021 ggcgcatttg ccagcggtaa cattctgcag gtgctgctgt ttgccgtact gtttggtttt
    10081 gcgctccacc gtctgggcag caaaggccaa ctgattttta acgtgatcga aagtttctcg
    10141 caggtcatct tcggcatcat caatatgatc atgcgtctgg cacctattgg tgcgctcggg
    10201 gcaatggcgt ttaccatcgg taaatacggc gtcggcacac tggtgcaact ggggcagctg
    10261 attatctgtt tctacattac ctgtatcctg tttgtggtgc tggtattggg ttcaatcgct
    10321 aaagcgactg gtttcagtat cttcaaattt atccgctaca tccgtgaaga actgctgatt
    10381 gtactgggga cttcatcttc cgagtcggcg ctgccgcgta tgctcgacaa gatggagaaa
    10441 ctcggctgcc gtaaatcggt ggtggggctg gtcatcccga caggctactc gtttaacctt
    10501 gatggcacat cgatatacct gacaatggcg gcggtgttta tcgcccaggc cactaacagt
    10561 cagatggata tcgtccacca aatcacgctg ttaatcgtgt tgctgctttc ttctaaaggg
    10621 gcggcagggg taacgggtag tggctttatc gtgctggcgg cgacgctctc tgcggtgggc
    10681 catttgccgg tagcgggtct ggcgctgatc ctcggtatcg accgctttat gtcagaagct
    10741 cgtgcgctaa ctaacctggt cggtaacggc gtagcgacca ttgtcgttgc taagtgggtg
    10801 aaagaactgg accacaaaaa actggacgat gtgctgaata atcgtgcgcc ggatggcaaa
    10861 acgcacgaat tatcctctta atctcaccac ttatgcccgc actcccttcc ccgagtgcgg
    10921 gtgtttgcgc ataattgacc cctgtgcctg aacttaccac ctgggtgagt tcaggtttat
    10981 ttcacttctt ccgtgacatt ttcatgttct tgcggtctaa cacgaagtgt ttttaacgtc
    11041 atttagactg accgacaacg ggcagttttt ttattaccag gatagttgat caggggttca
    11101 catgcagggc ataaaaattc gacttttagc gggcggtttg ctgatgatgg ccactgctgg
    11161 ctatgtgcag gcagatgcgc tccagcctga tccagcatgg caacagggga cgctttccaa
    11221 cggtttacag tggcaagtgc tgaccacccc ccagcgtccc agcgatcgtg ttgaaattcg
    11281 cctgctggtt aataccggtt cgctcgccga aagtacacaa cagagcggtt acagtcacgc
    11341 catccctcgt attgcgctaa cgcaaagcgg tggccttgac gcagcacagg cgcgttcatt
    11401 gtggcagcag gggatcgacc ctaaacgccc gatgccgccg gtaattgtct cttatgacac
    11461 cacgctgttt aatctgagtt tgcccaataa ccgtaacgat ttgctgaaag aagcgctctc
    11521 ttatctggca aatgccactg gcaaattgac catcacacca gaaaccatca accacgcgct
    11581 gcaaagtcag gacatggtgg caacctggcc tgccgatact aaagagggct ggtggcgcta
    11641 tcgtctgaaa gggtcaacct tgttaggtca cgatcctgcc gatccgctga aacaacccgt
    11701 tgaagcggaa aaaattaaag atttctatca gaaatggtac accccggatg caatgacgct
    11761 actggtggtg ggaaacgtgg atgcgcgctc ggttgtcgac caaatcaaca aaacgtttgg
    11821 cgaactgaaa ggcaaacgtg aaacgccagc tccggtgccg acgctttctc cgctgcgtgc
    11881 ggaagcggtg agtattatga ctgacgcggt gcgtcaggac cggttatcta tcatgtggga
    11941 tacgccgtgg cagccgattc gtgaatcagc cgcactgctg cgctactggc gtgcggacct
    12001 ggcccgcgag gcgctgttct ggcatgttca gcaagcgtta agcgccagtg acagcaaaga
    12061 catcggtctt ggatttgact gccgtgtgct gtatctgcgt gcgcagtgtg ccatcaacat
    12121 cgaatcacca aacgacaagc tgaacagcaa ccttaatctg gtggcgcgtg aactggcgaa
    12181 ggttcgcgat aaaggtctgc cggaagaaga gttcaatgcg ttagtggcgc aaaagaaact
    12241 ggagctgcag aaactgtttg ccgcctatgc acgggctgat accgatattc tgatgggcca
    12301 gcggatgcgt tcgttgcaaa atcaggtcgt cgatatcgcg ccggagcagt atcagaaact
    12361 gcggcaggat ttccttaaca gcctgactgt ggagatgtta aatcaggatc tgcgtcagca
    12421 gttgtcgaat gatatggcgt taatactgct gcagccgaaa ggcgagccgg aatttaacat
    12481 gaaagcgttg caggcggcct gggatcaaat catggcccca tctaccgccg ctgcgaccac
    12541 ctctgtcgcc acggatgacg tacatcctga agtgacggat attccacctg cacagtaatg
    12601 cctgatggtg atgagttagc acaaccatca ggtatttatg gatgagctgg atagaggggt
    12661 aacactttat ccagcctttt gcatatgctg cgtttacgct ggcatcgcct cacgcgggat
    12721 aatcgcgccg cgatactgaa taacggtact tgcggtcagg tgcccgcgtt tcgccgcgtt
    12781 ttccgcactg ccgcccgtca aacgtaccgc cagataaccg gcgctgaaag agtcgccagc
    12841 tgcggtggta tcgatcactt tttctttcgg cagtttcacc gccggaacat caactaacgc
    12901 ttcaccagca atggacacca ggcaagaatc cgccccgcgt ttcaccacca cttctttcac
    12961 gcccgcgtta tgggtgcgcg caatgacgtc ttccaccggc tgttgacccc acagcgcgtc
    13021 ttcgtcgtcc agcgtcagga aggcgatatc cgtgcattcc agcatttgct ggtacacctg
    13081 ctgtgtctct tctttgctgg cccacaggcg cggacgatag ttattgtcga aaatcacttt
    13141 tccgcctttg gcgcggcatt cgcgcagcag ggaaagcagc ttttcgcggc tggtcgggct
    13201 taagatcgcc aggctaatcc cgctcaggta gagataatcg aaattcgcca gctcttcgca
    13261 aatcgccgca gactgctcgc tcgccagcca gaatttggcg gcggcttcgt tacgccagta
    13321 gtagaacgta cgttcgccgg tgctgtcggt ttcaatgtag taaaggcccg gaagacggtt
    13381 ttccatccgt tgggtcaggg aagtatcaac gttctcgccg tgccaggcgt ccagcatctg
    13441 ctggctaaaa ctgtccgttc ccagcgccgt tacgtaatga acggttaatg ccgcaggatc
    13501 gacctgacgg gcgatataga cggaagtgtt cagggtatcg ccgccgaaac cgcgcttaac
    13561 gtccgcgcct ttctcggaaa gctcaatcat gcattcgcca atcacggcaa tctttttgga
    13621 catagtcgtg aacctgatct gtaaaaaatt agcgttagtt tgcgctgtgg tggtttgctg
    13681 gtcaaccata ttaaaacagt gttccattat ttttttgagc cagaacgtat tcctgaaaga
    13741 ttccgttgtg gagtgaggaa atttaccccg gccaggcggt aaagttctgc ccttacgcgc
    13801 cgataatctt tgtcgagtcc gggcagcatc acttttaaac acaggacatc tttgatgata
    13861 aggcaggtta tccagcgaat aagcaaccct gaagcaagca tcgagagctt gcaggaacgg
    13921 cgtttttggt tgcagtgtga gcgtgcttac acctggcagc cgatctatca aacatgcggg
    13981 cggttaatgg ccgtggagct attaacggtg gtcacgcatc ccttgaaccc ttcgcaacgc
    14041 ctgccgccgg atcgctattt tactgaaatc accgtcagcc atcggatgga ggttgtgaaa
    14101 gagcagattg atttgctggc gcaaaaagcc gacttctttg tagagcacgg cctgctggca
    14161 tcggtcaata ttgatggccc tacgctcatc gccctgcgtc agcaaccaaa aatcctgcgc
    14221 cagattgagc gtcttccctg gctgcgtttc gaactggtgg agcatatccg tctgccgaaa
    14281 gattcaacct ttgcctcgat gtgtgaattt ggcccgctgt ggctggatga ttttggtacc
    14341 gggatggcaa atttctctgc actaagtgaa gtgcgttatg actacatcaa aatcgcgcga
    14401 gaactgtttg tgatgctgcg tcagtcgccg gaaggccgca cactcttttc tcagctttta
    14461 catctaatga atcgctattg tcgcggggtg attgtcgagg gcgtagaaac accggaagag
    14521 tggcgtgatg ttcagaactc gcccgcattc gccgcacaag gctggtttct ttcacgcccg
    14581 gcaccgatgg aaacgctgaa tacggcggtt ctggcgctat aagctgcctc attttccgcc
    14641 gggctggact atctttagga ctggcacagg aagaatatga ggaagcgaac gatgagcaag
    14701 gcaggcaaaa taaccgctgc gatttcaggg gctttcttgt tgttgattgt cgtggcgatc
    14761 attttgattg caacttttga ctggaatcga ctcaaaccga ccatcaacca gaaagtctct
    14821 gcggagttga atcgtccgtt cgctatccgt ggcaatctgg gcgtggtgtg ggagcggcaa
    14881 aaacaagaaa ctggctggcg cagctgggtg ccgtggcctc atgtacatgc agaagacatc
    14941 attcttggta atccaccgga tattcccgaa gtcacgatgg tgcatttgcc gcgagtagag
    15001 gcaacgctgg caccgctggc gctgctgacc aaaacggtct ggctgccgtg gatcaagctc
    15061 gaaaagcccg acgcgcgcct gattcgcctc tctgaaaaga acaataactg gacgtttaat
    15121 cttgccaacg atgacaacaa agacgcgaat gcaaagccgt cggcatggtc gtttcggctg
    15181 gataatattc ttttcgatca agggcggatc gccattgatg acaaagtaag caaagcggat
    15241 ctggaaattt ttgttgatcc gttaggcaag ccgctgccat tcagcgaagt tactggatcg
    15301 aaaggtaaag cggataaaga aaaggtgggc gattacattt ttggcctgaa ggcgcaggga
    15361 cgttataacg gtgaaccgct cacgggtacg ggaaaaatag gcggtatgct ggcgctgcgt
    15421 ggcgaaggga cgccgtttcc ggtacaggct gatttccgtt caggtaatac ccgtgttgct
    15481 tttgatggcg tcgtgaatga cccaatgaag atgggcggtg tcgatttacg gcttaaattt
    15541 tctggcgatt cactgggtga tctctatgaa ctgacgggcg ttctgctgcc cgataccccg
    15601 ccgtttgaaa cggatggtcg gctggtagcg aaaatcgaca ctgaaaaatc gtcggtcttt
    15661 gattatcgcg gttttaatgg gcgaattggc gatagcgata tccacggttc tctggtctac
    15721 accaccggaa agccacgacc aaaactggaa ggtgatgtcg agtcgcggca attgcggctg
    15781 gcggacctgg gaccgttgat tggcgttgat tccgggaaag gggcagaaaa gtcgaaacgg
    15841 tctgaacaga agaagggcga aaaaagcgtt cagcctgcgg gcaaagtgct gccttatgac
    15901 cgcttcgaaa ccgataaatg ggacgttatg gatgccgatg ttcgcttcaa agggcggcgc
    15961 attgagcatg gcagtagcct gccgattagc gatctttcta ctcatatcat cctcaaaaat
    16021 gctgacctgc gcctgcaacc gctgaaattt ggcatggcgg gcggcagcat tgcggcgaat
    16081 attcatctgg aaggcgataa aaagccgatg caggggcggg cagatattca ggctcgtcga
    16141 ctgaaactga aagaactgat gcccgatgtg gaactgatgc agaagacgct gggggaaatg
    16201 aacggtgacg cggaactacg cggtagcggt aactcggtgg cggcgctttt aggcaacagt
    16261 aacggcaacc tgaaactgtt gatgaatgac gggctggtga gccgcaacct gatggagatt
    16321 gttgggctga atgtcggcaa ctacattgtc ggtgcgatat ttggtgacga tgaggtgcgg
    16381 gtgaactgcg cggcggcgaa tctgaatatt gccaacggcg tggcgcgccc gcagattttt
    16441 gctttcgata ctgagaacgc gttgattaat gttaccggca cggcaagttt tgcttcggaa
    16501 cagctggatt tgactattga tccggagagt aaagggattc ggattatcac actgcgttcg
    16561 ccgctgtatg tgcgtgggac gtttaaaaat ccgcaggctg gggtgaaagc cggaccgctg
    16621 attgcccgtg gtgctgttgc tgcggcactg gcaacgctgg taacaccggc ggcggcgtta
    16681 ctggcactga tctcaccttc cgaaggggag gctaatcagt gtcggacgat attgtcgcag
    16741 atgaagaagt gatgataatt gccggatgtc tgacatccgg caattaaaca gatttagatt
    16801 tttggcaaag tgtgctttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
    16861 atccttgtga attcaatata ttgtatggaa attcagtcct gataagcgta gcgcatcagg
    16921 cttttcacac ttattacaac gactgatgtc gcgtctcatg ggtcagcagc agggcgatta
    16981 acgtcaagcc agccatcgcc gccagatata accccaccgc acctaacccg tagttagcct
    17041 gcaaccaggc tgcgatatac ggcgcaacgg aagccccgag aatcgacgat acgttgtaag
    17101 agaacgatgc tccggtgtaa cgcacttctg tcggaaacag ctctggtaac agcgcaccca
    17161 tcggcccgaa ggtcagcccc atcagactta accccagcag caggaaggca aaaaccagaa
    17221 tcgggttgcc agaaccgagc agcgggttaa aggcgaacag cgcgaacagg atgatcagcg
    17281 tggtgatgat caccatgctt ttacggcgac caaaggcatc agccagtaat ccggcgactg
    17341 gcaccatcac gccaaaacca ataactgcca tcatcagcat ccacaacact tcgttacgcg
    17401 gcaggccaag cccaactggc gcggcggcgg tactgaaggt catagagtag accgtcatga
    17461 tgtaaaacag cgtataggtt gccagcataa tgaacgtacc cagtacggtt acgcgaacat
    17521 gtttggtcag cagcgtaccc agcgggatct tcacctgttt tttcgcttta gcgactttct
    17581 caaacaccgg cgactcatgc agcgacacgc gaacatacag gccgataatg accagcaccg
    17641 ccgagaagat aaatggcaca cgccagcccc agctcataaa ctgctcatcg gtcagtagcc
    17701 aggaaagcag caagaaagtg ccgttggcaa agaagaagcc aatcggtgcg cctagctgtg
    17761 ggaaggagcc atacagtgca cgtttgcgcg gtggggcgtt ttcagtcgcc agcagcgccg
    17821 cgccgcccca ttcaccgcct aagcccagac cctgaccaaa tcgagccagc gccagcagca
    17881 gtggggcgaa aataccaatc gtggcatagc ccggcagcag accaatcacc acggtcgaaa
    17941 tccccatcgt tagcaacgag gcgaccagcg tcgctttacg cccaacgcga tcgccaaaat
    18001 gaccaaaaac ggcagagcca atggggcgcg cgacgaaggc gatggcgaag gtggcgagcg
    18061 actgtagcgt tgctgccgtt ggatcgccct gcgggaagaa gatatgcgga aacacaataa
    18121 cggccgcagt ggcgtaaatg taaaagtcga agaactcaat ggccgtgcca atgagagagg
    18181 cgacaaggac tttattacgc gagttgatcg gcgtgtattc ttgttcgtgg tcgagtgttg
    18241 tggctgttgc ttgcataatc ttttcttatt ttttggctaa cgaatagcca tattacgcac
    18301 agcaaaagtg atatttcaat gtgtaacaaa tcgcggaaat ggtcaaaaac caggcaaaaa
    18361 ggggataatg tttaggccag tgattttt
//
LOCUS       DAZXUK010000055        18177 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZXUK010000055 DAZXUK010000000
VERSION     DAZXUK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18177
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.055"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            31..837
                     /gene="tcdA"
                     /locus_tag="ACH3L8_001546"
     CDS             31..837
                     /gene="tcdA"
                     /locus_tag="ACH3L8_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFT4084717.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(888..1331)
                     /gene="csdE"
                     /locus_tag="ACH3L8_001547"
     CDS             complement(888..1331)
                     /gene="csdE"
                     /locus_tag="ACH3L8_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFT4084718.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(1331..2536)
                     /gene="csdA"
                     /locus_tag="ACH3L8_001548"
     CDS             complement(1331..2536)
                     /gene="csdA"
                     /locus_tag="ACH3L8_001548"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFT4084719.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            2728..2955
                     /locus_tag="ACH3L8_001549"
     CDS             2728..2955
                     /locus_tag="ACH3L8_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFT4084720.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            3306..4223
                     /gene="gcvA"
                     /locus_tag="ACH3L8_001550"
     CDS             3306..4223
                     /gene="gcvA"
                     /locus_tag="ACH3L8_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFT4084721.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            4242..4637
                     /locus_tag="ACH3L8_001551"
     CDS             4242..4637
                     /locus_tag="ACH3L8_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFT4084722.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            4630..5730
                     /gene="rlmM"
                     /locus_tag="ACH3L8_001552"
     CDS             4630..5730
                     /gene="rlmM"
                     /locus_tag="ACH3L8_001552"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFT4084723.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(5774..6505)
                     /gene="fucR"
                     /locus_tag="ACH3L8_001553"
     CDS             complement(5774..6505)
                     /gene="fucR"
                     /locus_tag="ACH3L8_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFT4084724.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(6563..6985)
                     /gene="fucU"
                     /locus_tag="ACH3L8_001554"
     CDS             complement(6563..6985)
                     /gene="fucU"
                     /locus_tag="ACH3L8_001554"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFT4084725.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(6987..8406)
                     /gene="fucK"
                     /locus_tag="ACH3L8_001555"
                     /pseudo
     CDS             complement(6987..8406)
                     /gene="fucK"
                     /locus_tag="ACH3L8_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(8515..10290)
                     /gene="fucI"
                     /locus_tag="ACH3L8_001556"
     CDS             complement(8515..10290)
                     /gene="fucI"
                     /locus_tag="ACH3L8_001556"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFT4084726.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(10323..11639)
                     /gene="fucP"
                     /locus_tag="ACH3L8_001557"
     CDS             complement(10323..11639)
                     /gene="fucP"
                     /locus_tag="ACH3L8_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFT4084727.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            12186..12833
                     /gene="fucA"
                     /locus_tag="ACH3L8_001558"
     CDS             12186..12833
                     /gene="fucA"
                     /locus_tag="ACH3L8_001558"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFT4084728.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            12861..14009
                     /gene="fucO"
                     /locus_tag="ACH3L8_001559"
     CDS             12861..14009
                     /gene="fucO"
                     /locus_tag="ACH3L8_001559"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFT4084729.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(14062..14817)
                     /gene="xni"
                     /locus_tag="ACH3L8_001560"
     CDS             complement(14062..14817)
                     /gene="xni"
                     /locus_tag="ACH3L8_001560"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFT4084730.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(14928..16295)
                     /gene="sdaB"
                     /locus_tag="ACH3L8_001561"
     CDS             complement(14928..16295)
                     /gene="sdaB"
                     /locus_tag="ACH3L8_001561"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFT4084731.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(16353..17642)
                     /gene="sdaC"
                     /locus_tag="ACH3L8_001562"
     CDS             complement(16353..17642)
                     /gene="sdaC"
                     /locus_tag="ACH3L8_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFT4084732.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4503 a         4465 c         4781 g         4428 t
ORIGIN      
        1 taacctcgcc ctttttaatt ctgaggtgct atgtctgtgg taattagtga tgcatggcgt
       61 cagcgttttg gtggcacagc gcgtctgtat ggtgaaaaag cgttgcaact gtttgctgac
      121 gcgcatattt gtgtggttgg tatcggtggt gtcggttcct gggcggcgga agcgctggcg
      181 cgcacgggga ttggcgcaat cacgcttatc gatatggatg atgtgtgcgt caccaatacc
      241 aatcggcaaa ttcacgctct gcgcgataac gtcgggctgg caaaagcgga agtcatggcg
      301 gagcgcattc gccagattaa cccggagtgc cgtgtaacgg tggtggatga tttcgtgacg
      361 ccggataacg tagcgcaata tatgagtgcc ggttattcgt acgtgattga tgccattgat
      421 agtgtacggc ccaaagcggc gctgattgct tattgtcggc gcaataaaat cccgctggtc
      481 acgaccggtg gcgcgggtgg gcagattgac ccgacgcaga ttcaggttac cgatctggcg
      541 aaaacgattc aggatccgct ggcggcgaag ttgcgcgagc gcctgaaaag cgattttggc
      601 gtagtgaaaa acagtaaagg taagctcggc gtggattgcg tgttttctac tgaagcgctg
      661 gtgtacccgc agtcagacgg tacggtgtgt gcgatgaaag ccacggcaga agggccgaag
      721 cggatggatt gtgcatctgg atttggcgcg gcaacgatgg tgaccgccac ctttgggttt
      781 gttgcggttt ctcatgcgct gaagaagatg atggcgaaag cggcgcgtca gggttaaaca
      841 atcaggtcgg atgagacgcg aacgtcttat ccgacccggt tctgtgctta aacctgcttc
      901 gtcgcagcga taatcgcctc gcttaacgca tttaacccct ggctgcgtga ggcgctaagc
      961 tgcgcacgta atcccagctc atcaaacaat gccagcggcg actgtgcctg taattcggcg
     1021 gcggttttcc cctcaacggc agtcaacaac accgccaaca ggccgcgcac aatgcgccct
     1081 tcgctgttgc caaagaagtg cattttgccg ttttcagcca cggtatatcc cagccagacg
     1141 cggttttcgc atccggcaat ctctttagcc tgcgctttta actcgtctgg caaagccgga
     1201 agctgtttcc ccagcatgat caactggcga tatttatctt cccattgcga caacggtgcg
     1261 aaggtattgc gtaacgtttc tgcggttacg gttgtgccga acggatgtcc ggcgaattgc
     1321 gggtttgtca ttaatccacc aataattcca gcgcgcggtc aacggcattc accagcgcat
     1381 ccacatcact ctttgtatta tatggcgcaa atgaggcgcg cagtgtgccg gttacgccta
     1441 attctgccag tagcggctga gcgcaatgtt gcccggcccg caaggcaata ccgtactccg
     1501 ccagtagcgt caccatatcg ctgtgatgaa cgccagcaaa atcaaaggcc agcaggctgg
     1561 aatcctggca gcggaatgaa cgaaagcctg gacgtttcgc cagcgcttct tctgcaagcg
     1621 ttgctaagct acggctccag ctttcggcct ggttgatatc gtaatctgcc agccattcca
     1681 gcgccgcgct taatcctatg acgccagcga catttggcgt tcctgcttcc agtttccacg
     1741 gcgcagattg agtggtgaaa ccgtcaaaac tcacttcgtg aatcattttg ccgccaccca
     1801 gccagggcga catcgcttcc agcagttctg atttgccata cagcacgccg atacccgtcg
     1861 ggccatacag tttgtgacca gagaaagcat agaaatcgat atccagttgc tgaacatccg
     1921 cggggaaatg caccgtcccc tgagcaccat caaccatcac caccatcccg gctgaatgag
     1981 caaaggtaat cgctcgcgcc agatccgggc aaccgccagt cacgttcgac atctgaccca
     2041 acgccagaat ccgactacgg ggagtaatca gctctggcaa caaatcgaca tccggcagtc
     2101 gctgcgcatt aagcggcaat ttcaccactt tggctctagt ttgttgggcg accatcagcc
     2161 aggggacgag gttagcgtgg tgttctgcca cgctgacaat aatctcatcg cccggttgca
     2221 gacgcggacg cgcatagcat tgtgccacca tgttgatgga ttcagtggtg ccgcgcgtcc
     2281 agacgatagt tttatcatcc ggtgcattca gtaattgcgc cactttctct cgtgcagctt
     2341 cataacgcgc ggtcaggcgt tgggcttcgg caaactggct gcgatggacg tttccggcgc
     2401 tcaggctgta aaactgtcga gtggcttcaa ccacggcttc aggtttaagc gcggtcgcgg
     2461 cgctgtcgag atagacgccc gcatcctgta gtgcgggaaa ctgggcgcga aactgcgcgg
     2521 gattaaaaac gttcatggta ctcctcggct tgatgcaccg atcgtgacgc aaaattcact
     2581 ttgatacaag gagagtgcta accatcagaa ttatctggat atcctggcga agccgcgtaa
     2641 gtaacatatg ctgaatagtg ttaggcgtta attttagcct gttaatcaat actattaaga
     2701 tttattaatc gttttaagga gaagatgatg aaaaagactg ccgcaattat ttctgcctgt
     2761 atgctgactt ttgccctgag cgcctgttcc ggttcgaact atgtgatgca caccaatgac
     2821 ggacgtacca tcgtctctga cggcaaacca cagactgata acgatatcgg tatgatttcg
     2881 tataaagacg ctaatggcaa caaacagcag atcaaccgta ctgacgtgaa agagatggtc
     2941 gaactggaac agtaattcgc gatcgcaagg taaaaaaaag caccgcaatt aggcggtgct
     3001 acattaatca ctatggacag acagggtaaa tgtacaggaa gtgaaaaaag gtagctttgc
     3061 taccatggtc tgaatcgcag accaattgca aacacaacaa cacaacatca caaccgtaag
     3121 ccaaaagttc accagaatac gcattccgat aaaacttttc gttccggctc aggaagtgcc
     3181 gccactatag gtatttgctg gtagaagctc aacggacaat ttataatggc tcagattaaa
     3241 aaaactaata ggttacacag tgtgatctaa ttgttaaatt catttaacat caaagtttaa
     3301 aagccatgtc taaacgatta ccaccgctaa atgccttacg agtttttgat gccgcagcac
     3361 gccatttaag ttttactcgc gcagcagaag agctttttgt gacccaggcc gcagtaagtc
     3421 atcaaatcaa gtctcttgag gattttttgg ggctaaaact gttccgccgc cgtaatcgtt
     3481 cactcctgct gaccgaggaa gggcaaagct atttcctcga tatcaaagag atattttcgc
     3541 aattaaccga agcgacgcgt aaactccagg cccgtagcgc caagggggcg ttgacggtca
     3601 gtttactccc cagtttcgcc attcattggt tggttccgcg actttccagc tttaattcag
     3661 cttatccggg aattgacgtt cgaatccagg cggttgatcg tcaggaagat aagctggcgg
     3721 atgatgttga tgtggcgata ttttatggtc ggggcaactg gccggggcta cgggtggaaa
     3781 aactgtacgc cgaatattta ttgccggtgt gttcgccgct actgctgaca ggcgaaaaac
     3841 ccttgaagac cccggaagat ctggctaaac atacgttatt acatgatgcg tcacgccgtg
     3901 actggcagac atatacccga cagttggggt ttaatcatat caacgttcag caagggccaa
     3961 tttttagtca tagcgccatg gtgctgcaag cggctattca cgggcaggga gtggcgctgg
     4021 caaataacgt gatggcgcaa tctgaaatcg aggccggacg tcttgtttgc ccgtttaatg
     4081 atgttctggt cagtaaaaat gctttttatc tggtttgtca tgacagccag gcagaactgg
     4141 gtaaaatagc cgcctttcgc caatggatcc tggcgaaagc cgctgctgaa caagaaaaat
     4201 tccgctttcg ttatgaacaa taatttacgt agggtacgac catgaccagc cgttttatgc
     4261 tgattttcgc cgccattagc ggcttcattt ttgtggcact gggcgctttt ggtgcgcatg
     4321 tgttaagtaa aaccatgggg gccgttgaga tgggctggat ccaaaccggt ctcgaatacc
     4381 aggcgtttca tacgctggcg atcttaggtc tggcggtggc aatgcagcgt cgcatcagta
     4441 tctggtttta ctggagtagc gttttcctcg cgttaggcac ggtgctgttc agcggcagcc
     4501 tttattgcct ggcgctgtcc catctgcgtt tgtgggcgtt tgtcacaccg gttggcggcg
     4561 tgagcttcct tgcgggctgg gcgttaatgt tagttggtgc tatccgttta aagcgcaagg
     4621 gcgtaagtca tgaataaggt tgtattgctg tgccgtccgg gctttgaaaa agagtgcgcc
     4681 gcagaaatta ccgataaagc cggccagcgg gaaattttcg gttttgcccg cgtgaaagag
     4741 aatgcgggtt atgtcattta tgaatgttat caacctgatg atggcgataa gttaatccgt
     4801 gagctgccgt tcagttcatt aatttttgcc cgccagtggt ttgtggtggg ggaactcctg
     4861 cagcatttgc cgccagaaga tcgtattacc cccattgtcg gcatgttaca gggtgtagta
     4921 gagaagggcg gtgaactgcg tgttgaagtt gccgatacca acgaaagcaa agagttgctg
     4981 aaattctgcc gtaagttcac tgttccgcta cgcgctgcct tgcgcgatgc tggggtgctg
     5041 gcgaactatg aaacgccgaa gcgtccggtt gtgcatgtat tctttattgc accaggatgc
     5101 tgctataccg gttactcata cagcaacaat aattcgccgt tctatatggg cattccgcgc
     5161 ctgaaatttc cggcagaggc gccgagtcgt tccacgctca aactggaaga ggcatttcat
     5221 gtgtttattc ctgcggatga gtgggatgaa cgcctggcga acgggatgtg ggcggtggat
     5281 ttaggcgctt gccctggcgg ctggacctac caactggtga agcgcaacat gtgggtttat
     5341 tccgtcgaca acggcccgat ggcgcaaagt ctgatggata ccgggcaggt gacgtggctg
     5401 cgggaagacg gtttcaaatt ccgtccgaca cgcagcaata tctcctggat ggtatgcgat
     5461 atggttgaaa aaccggctaa agttgcggca ttgatggcgc agtggctggt taatggctgg
     5521 tgccgtgaaa ccattttcaa tctcaaactg ccgatgaaaa aacgctacga agaagtatca
     5581 cacaatctgg cgtatattca ggcacagctt gatgaacatg gcataaatgc tcagattcag
     5641 gcgcggcagt tgtatcacga tcgcgaagaa gtgacggtgc acgtccgccg catctgggct
     5701 gcggtgggtg gtcgtcgcga cgagcgataa caggcaaggc cggggcgtcg tctccggcct
     5761 gctacccttt ccgtcaggct gttaccaaag aagttgcaac ctggcgctca tcagaaataa
     5821 tgtgcgttac ctcatccaga tgcccgatgc gggcttcccc tgaacgatta aatttacttt
     5881 tatcaatcaa taacaacgat tgcgcggcac gttttaatag catcgatttg taatcagcgt
     5941 tgatcgcatt ggagtcccac agtgcgccgc tgctatcgat cccttcacaa gaaaaaataa
     6001 acagatcgat ttccagcgat ttaagttggg aaatcagcga gggattgacg taacagccat
     6061 atttgcgctc aagtgtgccg ccggaactga tcagttgaat gcgttcgcgt ttaccgagtt
     6121 catggcaaat cggatggcta ttggtgaaga cctgaatgtt gatgtcaggc aactggcgtg
     6181 ccagatacca gcaagttgaa ctggcgtcta aggctatcac catcccttct tcaatccacg
     6241 cgagcgcctc gcgcgcgata tctgctttat gcgcataatg gcttttcagc ctgatgtgaa
     6301 agggatcgcc actgtcttga ttttgacggt ggatatattt agcgcgtcca tgattgcgca
     6361 gaattttacc ctgcgtctgt aattcattga gatcgcgacg aatggtttct ttactgacct
     6421 ttagctgttc agagagagct tccgtggtca ggctggtatg gttcagcagc aggtcgacta
     6481 tcgcttgctg gcgtgccgct ttcatatttt tcaccccatc attcgataac cgcccgcgtc
     6541 atgcgggcgt accggtatga gattacggtg ttaccccttt ttttaaaaga atattcccgt
     6601 acttcgctcg ttcgcctgtg ataacgatcg caaaggcttt ttgcgcccgt tcataaaacg
     6661 caaaacgatt gatgcggatg atgtcaggac acggggcttg tagtgaaagc gcattacggt
     6721 aacgtcgttc tacttcagga tcgagagtgt caccctctac cgccgccatc atcaccagcg
     6781 gcggggcata actgtccagc tcaaataacg ggataatcgc ctggagcaag tcgctcacca
     6841 acaggccatc agcgcggatc acctgcggtt ccatcgaatg ggcgggaaag tgagcatcgg
     6901 aaaaaataat ttcatctcca tgtcccatct ctgccagcac tttcaatagt tcgggagaaa
     6961 ttaacggcga aattgttttc agcatttcac acttcctcta taaattcagg ttcagtttgc
     7021 gggtagaaat aacggtactg ataatgaatc tgtgcacggg cttcttccgg gctgttaaat
     7081 tcccctacgc cataccaacc gaacagcgca gctcctgcga cggtcgtttc ggcatcgtcg
     7141 agaactttta ccggaatatc aagcatattg gctttaatct gattccacaa tgtgttgcga
     7201 cttcctccac cgactaacaa taattcatag gctttaaagt gcccgatttt ttccagcatc
     7261 tgtagattgc gctgtaattg cgcagttaac ccttccagcg ccgcgcgata gaaatgcccc
     7321 cgcgtggtat taagcgtcac tccttgccag ccagcgttct gacacgacaa taaatcacac
     7381 tgcattttca caccatccgc gccaggtgcg atcagacgag cttcttcaat caacatttgc
     7441 cagggtgttt cagccgtcca gaacagtttt ctcacccatt ccagcacgcc ggatgccagc
     7501 cattgcatac ctgggttata caaccatgcc tggctatcta gttcgcaggt ggaaccggcg
     7561 tactgactta acagcgaagt atcaacctag gcgctgcgaa ccattaaaat ttcccatgta
     7621 ccggaggaga gcacgggttc attttgttcg gcaccagcgc caaaaagggc gaactgggta
     7681 tcgtgacctg cggaaatcac cggtatgcca acgggtaagc cgagcattgc tgcggcgttg
     7741 ttctgtagcg taccaatctg ttcacccgct tccaccagac gagggaagag tcggcgtgga
     7801 ataccggtgg cttgtaaaat ttgcggactg aaatcgcgtt gctggatatc cagcatctgg
     7861 ctggttccgg ccatcgtgat atcagtagtg aattcgctgg ttaaacggtg gttaatcagc
     7921 gacgaaatga agagccaggc gtgcgcgcgt tccagcagtt gtggatgatt ttctttcaac
     7981 cacaccaact tatataacgt attgaaacta aaggctccga cgccagaaat agcctgcaac
     8041 cgctgtgcgg agattaaccg ttcaatattg tccataaccg ctgctgttcg cggacatttc
     8101 cagctaatga tcggatagag cagattgcct tgcttatcta ccagagcgcc atccacacta
     8161 aaggtggtga cggcgatacc gcggatgtgg cattcagtca gttcactatt gatttgccga
     8221 cagcaatcag caaagcgttg caaaatggca gtctaaagac cactggtgcc aggtgttgtt
     8281 ttccatcgcg atatcgctgg cattaggcgt tgaggcgcgg gcaacaattt ttccctgccg
     8341 attaaccgcg atggccctga cattggtcgc gccacagtcg agtaccagga taacttcttg
     8401 tttcataatc gctcctgcaa tatagccgga taacattgtt tatccggcta accactcttg
     8461 cttgttcagg cccggtagcc ggagcgaccg ggcatcacat cagggtgtaa tgtattaacg
     8521 cttgtacaac ggaccgtagt tctggcaagc gcggtaatcc tggccttcaa tatccatgcc
     8581 gtgcgcagcc caggcagaag gacgatacac tttggtctct tcaacgttgt gcatacatac
     8641 cgggatacgc agcatggagg caagggtgat aaagtctgcg ccaacgtggc cgatggtcag
     8701 aactccgtgg ttggcacccc agttcgccat taccgaatac acatccgtaa acggcccttt
     8761 accagtgagg cgcggtgcaa accaggtggt tggccaggtt gagttggtgc gtttgttgag
     8821 gatgtcatgc acatccttcg gcaattccac gctccagcct tccgcgattt gcagtaccgg
     8881 tcccaggcct ttgatgatgt tgacacgagt catggtgaac gggacgccgc cttcggtaag
     8941 gaagcgggaa gagtaaccgc cgccacggaa gtattcgtgg atcgccgggc accattcggt
     9001 agcggcgagg caagcgtcag cctcttgctg agagatttcc cagtgcggct tcatcgtcgg
     9061 gttaccttcg ctgtcgcgtt gtttacagga accgtccagc gcagcagaac ctgagttgat
     9121 caaatggatg atgccgtgtt ctgccagtcc gtccagttta tgccccgtta cacgctcaat
     9181 tgcttctggt gaccagtagg tacgcacatc ggcaaatacc tgagcggtgc cggtgagctg
     9241 gtgacccatt agcattgcca cgccgttaag actgtcgttt tcggtcgcca cgacaaaggg
     9301 ttcgcgcacg ccattccagt caaatgaact gttgaggatc gcttcggcgg tgtcaccatt
     9361 gggatattga tcggtccagt gacgttgccc ctggaagccc gcagcgatgg cgttgtagcc
     9421 aagtgattct tccacgcgac caatatcggc cagtttgctg ttgccttgca tcatgtcgcg
     9481 gatacacatc gccatcagta aactttcgcg cagaactgcg cggctttgct cggcattgcg
     9541 ttgatactgt ttgttatttt catcttcgcc atagcggaag tttttatcag cccaggccag
     9601 tgccatttcc agttcggctt cgtcgtaaat cttctgatcg atacggcgac gcagttcggt
     9661 catatccacc gcctggactt tcattcccag ccaggattca aagaagttgt gatcaacaat
     9721 agaaccggcg atacccatcg aaacgccgcc cagcgacaga tagcttttac ctttcatgct
     9781 ggcgacggcc aaaccggcgc gggcaaagcg cagcagtttt tcttcaacat cggcaggaat
     9841 cgatgtgtca tcggcatcct gaacgtcatg accgtaaatg gagaatgccg ggatgccttt
     9901 ctggctgtga gctgccagag ccgcagccag gtaaacagcg ccggggcgtt cagtgccgtt
     9961 aaagccccaa atggctttcg ggcgggttgg atccatgtcg atggtttcac tgccatagca
    10021 ccagcaaggc gttaccgtaa tggtgaggcc tacattctgg ctgctgaatt tttcttcgca
    10081 agcagcggct tcagccatac ccgcgataca ggtatcggaa atgacacact cgacggcagc
    10141 tccgcaggca tggcgcagtt tctcggtcag cagtgcggcc gtagctttcg ccatattcat
    10201 tgtttgttct tcaagcgact cacgaacacc catgcgacga ccgtcaataa ccgggcggat
    10261 accaattttc ggtaagctga tttttttcat tacagattcc tcactttatt cggaaaatat
    10321 gttcagttag ttgccgtttg agaacggaaa cgggcaaaga taaagatgac cgcgaagcag
    10381 agtgcgggga tcagttcagc agtggggatg ttgcccgccg cgtcactgac aaaacccatg
    10441 accggagtga caataccgcc gccaataatg gtcataacga tgaaggacga accgtatttg
    10501 gtgtcctggc cgagattctt aatgcccagc gagaagattg ttgggtactg aatcgacata
    10561 aaggcgctgc ataaagtcag ggctattaag cccacatgac cgccagcgaa ggctgagatc
    10621 aggcacagtg ccatagcgat taatgcgtag gcggccagga ctttgtgtgg tgcgaagcga
    10681 ctgatgagcc aggtaccggt gaaacgacca ataaagaagc acaccatggt tccggttaaa
    10741 tagttagcgg caaagcctgc agtcatacct agaatttctt ctacagcgta gcgaatcaaa
    10801 tagctccagc aggccgtttg tgcgccgaca tagcagaatt gcgctaatac cgcccagcgc
    10861 cagtgacgaa tacgcgccag acgagaaagc gatgcggaga acgatccttg tttggcgtca
    10921 ctgtgattat cactctgcaa tgccgggaat ttcgtcagca tgatcagcag ggcgaccagt
    10981 aacacgatag ccacgatgat catataaggt gtctgtaccg ataataccag gctgtgttta
    11041 tacgcactca attgctctgg agacatttta tcgagaacgt cttgcgattg atgtggcacg
    11101 ttagacaaaa taagactttg cccaaagaca accgcgataa ttgcgccaaa cgagttaaat
    11161 gtttgcgcaa gatttaagcg gaagtgacta ctgctttccg gccctaatac cgtaacaaaa
    11221 gggtttgcgg cagtttccag acaacctaat ccggctgcaa taataaatag gccaactaaa
    11281 aataaggtgt agttcattat ttctgcagcg ggccagaata atgcagcgcc caaggcatat
    11341 aaaaataatc cggtaataat ccctgcttta taactgagtt ttttcatcaa tatcccagca
    11401 gggattggga taatgaaata accaaagtaa aaggccgatt ggatcaggcc agcctggaaa
    11461 tttgtcagcg taaaagcctg ctggaattga ggtaataaaa tgtcgttaag gttattggct
    11521 accgcccaaa gaaaaaacag tgagcacagc agcgcgaatg gaataatgta acttctgctt
    11581 tgccctgcat ctttatctac cgcacggtaa ctctgcgttt gtattgatgt gtttcccata
    11641 gcatcctctt aggttcagaa gcttaagcac gtaatcggtt atgttttcaa aagtgcaaaa
    11701 ccagcgagtt cgggcttcat atcggtgaat atttgcctgt ctgaaatgaa ctcatggatt
    11761 cattttcaaa taaaaaacgc tctgtaatat gacggcggtc acacttatta gctggaaggt
    11821 gactttatgt gactaccatc actttaataa tcgaaatttc atgcccgttt cagaaaataa
    11881 atcataaaaa acacaaacca gatgaccgta ttaattgttt gtgccgaaaa cgggcatttc
    11941 tggcgagggg aaatggaaaa atgaccgttt tgataaatat ttttaagagc ggtcattttt
    12001 aacggggtta taaaaagaga acttgcagtt atttactgtg attagttgag ctaggtcaca
    12061 aaatataagc ttgtgaattt ctttcttgtt aatcaggaag aagatggtgg gtaattaaac
    12121 ggctaattca atagtgtgaa aggaacaaca ttattgccct gttttgaatc agagagaggt
    12181 agctaatgga acgaaataaa cttgctcgtc agattattga cacttgcctg gaaatgaccc
    12241 gcctgggact gaaccagggg acagcgggga acgtcagtgt acgttatcag gatgggatgc
    12301 tgattacgcc tacaggcatt ccatatgaaa aactgacgga gtcgcatatt gtctttattg
    12361 atggcaacgg taaacatgag gaaggaaagc tcccctcaag cgaatggcgt ttccatatgg
    12421 cagcctatca aagcagaccg gatgccaacg cggttgttca caatcatgcc gttcattgca
    12481 cggcagtttc cattcttaac cgatcgatcc ccgctattca ctacatgatt gcggcggctg
    12541 gcggtaattc tattccttgc gcgccttatg cgacctttgg aacacgcgaa ctttctgaac
    12601 atgttgcgct ggctctcaaa aatcgtaagg caactttgtt acaacatcat gggcttatcg
    12661 cttgtgaggt gaatctggaa aaagcgttat ggctggcgca tgaagttgaa gtgctggcgc
    12721 aactttacct gacgaccctg gcgattacgg acccggtgcc agtgctgagc gatgaagaga
    12781 ttgccgtagt gctggagaaa ttcaaaacct atgggttacg gattgaagag taatttcgta
    12841 aagcaacaag gagaaggatg atggctaaca gaatgattct gaacgaaacg gcatggtttg
    12901 gtcggggtgc tgttggggct ttaaccgatg aggtgaaacg ccgtggttat cagaaggcgc
    12961 tgatcgtcac cgataaaacg ctggtgcaat gcggcgtggt ggcgaaagtg accgataaga
    13021 tggatgctgc agggctggca tgggcgattt acgacggcgt agtgcccaac ccaacaatta
    13081 ctgtcgtcaa agaagggctc ggtgtattcc agaatagcgg cgcggattac ctgatcgcta
    13141 ttggtggtgg ttctccacag gatacttgta aagcaattgg cattatcagc aacaacccgg
    13201 agtttgccga tgtgcgtagc ctggaagggc tttccccgac caataaaccc agtgtaccga
    13261 ttctggcaat ccccaccaca gcaggtactg cggcagaagt gaccattaac tacgtgatca
    13321 ctgacgaaga aaaacggcgc aagtttgttt gcgttgatcc gcatgatatc ccgcaggtgg
    13381 cgtttattga tgctgacatg atggatggta tgcctccggc gctgaaagcc gcgacgggtg
    13441 ttgatgcgct cactcatgct attgaggggt atattacccg tggcgcgtgg gcactaaccg
    13501 atgcactgca cattaaagcg attgaaatca ttgctggggc gctgcgagga tcggttgctg
    13561 gtgataagga tgccggagaa gaaatggcgc tcgggcagta tgttgcgggt atgggcttct
    13621 cgaatgttgg gttagggttg gtgcatggta tggcgcatcc actgggcgcg ttttataaca
    13681 ctccacacgg tgttgcgaac gccatcctgt taccgcatgt catgcgctat aacgctgact
    13741 ttaccggtga gaagtaccgc gatatcgcgc gcgttatggg cgtgaaagtg gaaggtatga
    13801 gcctggaagg ggcgcgtaat gccgctgttg aagcggtgtt tgccctcaac cgtgatgtcg
    13861 gtattccgcc acatttgcgt gatgttggtg tacgcaagga agacatttcg gcactggctc
    13921 aggcggcact ggatgatgtt tgtaccggtg gcaacccgcg tgaagcaacg cttgaggata
    13981 ttgtagagct ttaccatacc gcctggtaaa tgcgctgatg tgataatgcc ggatacgatt
    14041 tgcggcgtat ccggctcgcc gttaccgtac caaccgcaat tgctgtaaat tgccgtcgat
    14101 atgcaaatcg gtttgtaagc gggcaatatc gcggcacaga aacgccatct ctttatgggt
    14161 ttctaatttt ttgcgccact tttcggcaac cgcatccaga ttctcatata tcccttccag
    14221 actctgaaac tcgaccagca gctgcgtggc gctttttggt ccgattcccg caacacccgg
    14281 tacctttgaa ctgctgatcc ccgcaagtcc ccagtaatcg ggcaactgct gcggttgaac
    14341 gccaaattct ttatcgataa atggcgcatc cagccaacgt ttctggaagt aatcacgaat
    14401 acgtaatgtc ggtgaaagta actgacagta gcctttatct gtcgaaacaa tcgttgcctg
    14461 atgcccggcc tgtgtcactt tgaccgccag tgtggcggct aagtcatcgg cttcgttgcc
    14521 gctggctgac cagcacggga cgccgcgttg ctcaaaggcg gcgcgtaatg caggcatctc
    14581 gtcgtgcaac tcttccggca ttggcggtcg atccgctttg taatctggta aacgctgatg
    14641 acgccagccg ctactgcggt tttcatcatc aaaaacggcg accgcgtggg ttggctggct
    14701 gtgcataatg agctgatcga gcgcatgctg gcaggtttcg acacagggcg aaccctgaac
    14761 ggcatgaatg cgacggataa gattcagtgc atcgacaata agcaaatgaa cagccacgat
    14821 aaccccctta aagatcaggc gataagggta acattcgatg ccggaacagg ctatggctgg
    14881 ttgagaaaac aggaagaggc ttcgcaaaac gaggcctctg ggagagatta atcgcaggca
    14941 acgatcttca tcgccaggcc gccgcgagag gtttcgcggt acttggcgtt catgtcttta
    15001 cctgtttcgt acatggtttc gataacttta tcgaggcaga cgcgcggttc gctggtacgg
    15061 cgcagtgcca tacgcgcggc gttcaccgct ttcactgctg caatggcgtt acgctcaatg
    15121 cacggtacct gaacctgccc cgcgaccggg tcacaagtca gaccgaggtt gtgctccatc
    15181 gcgatttccg ctgcgatgca aacctgcgcc gggcttgcgc ctaacagttc tgccagacca
    15241 gctgccgcca tagagcacgc cacgccaact tcaccctggc aacccacttc ggcaccagaa
    15301 atcgacgcgt tcatcttata aagagaacca atggcgctgg ctaccagcag gtaacgagcc
    15361 agtgagttag cgttcacttc gcggataaac ttatcgtagt acgccagcac agccgggata
    15421 atcccgcacg caccgttagt cggcgcagtc accacgcgac cgccagcagc gttctcttcg
    15481 ttcactgcca gtgcaaacat gttgatccag tcaacaaccg ccatcgggtc agtggtggtt
    15541 ttatcctggc tgaccagcat ccggcgtagt gccgcagcac ggcgtggaac gcgcagtttg
    15601 ccaggcaaca cgccttcggt ggaaataccg cgctcaatac cgccacgcat gacttcccag
    15661 acgttcgcca ggtgctgttc cagctcttct ttactgtgca gcgccagctc gtttttcatc
    15721 atcaggccag agagtgacag cccggtttcc tgacaatgtt tttgcagatc ggctgctgaa
    15781 ctgtacggat aaggaacttc aaccggtgcg ctatcctgct ggccaaaatg ctcttcatca
    15841 acgataaagc caccgccaat agagtagtaa gtctggctgt aaacgacttt atcgcccgcc
    15901 agcgcggtaa tgcgcatacc gttttcatgc agagaaaggt tgtctgcatg gaagttcatg
    15961 cactgatcaa ccgggaactc cacttcatgc tgaccgtttg ccagcatcag gcgaccatga
    16021 gtattcacat cctgaataaa accggggatg gaatcgatat ccacggtatc cggcaggtta
    16081 cccgccaggc ccataataat ggcgatatct gtgtggtggc ctttaccagt cagagagagc
    16141 gagccgtaca cgtcaaccac cacgcgggtc acgtctttaa gcaggttacg ggcaatcaga
    16201 tcgtcggtaa attgtttacc cgctttcatt ggtccaacgg tatgagaact ggaagggcca
    16261 atgccgattt tgaaaatatc gaatacgcta atcataggaa atacatcgcg ttaaaacgga
    16321 ggaagcgccg cccgaaagcg gcgcgaaagg acttagctga acagagagta gaagattgcg
    16381 gagattgcaa tcagacccat cacgacaacg aatacgttgc tgatgtgacc gctgtactta
    16441 cgcattgccg gtactttctg aattgcgtac atcggcatca ggaacaggat catcgcgatg
    16501 attgggccgc ccagggtttc aatcataccc aggatgctcg ggttcagggt ggcaacaatc
    16561 caggtcgtta ccagcatgaa cagcgcagtg atacggttca gcttgttgat ttcgatagac
    16621 ttacctttac cacgcagaga tttaatcacc ataccgttga agccttcacg tgcgcccagg
    16681 tagtggccga ggaaggattt ggtgatagcg ataatcgcga taatcggagc catccacgcg
    16741 ataaccggtg cgttaaagtg gttagccagg taagacagaa tcgagatgtt ctgctcttta
    16801 gccgcagcca ggtctgccgg agtcaggctc agtacgcagc tgaagacgaa gaacattacg
    16861 gtcagcacca tcatgatgtg tgcgaatgcc aggatcttcg agcatttctg ttctgccata
    16921 tcgccgtact cttcacgctt cgcaacggcg aaagaagaga tgatcggaga gtggttgaac
    16981 gagaacacca ttaccggaat tgccagccac agggtcatcc acagaccgtt tccggttgca
    17041 gatgcagtgt ccagagacag cgtttccagt gctgcgccgt tccactgcgg gatcaggtac
    17101 agagccagca gcatcagtac gccaacaaac gggaatacca gaatactcat cgctttaacg
    17161 atcatctgct caccaaagcg aacgatggtc atcataccca cgatcaggat cagcgacaga
    17221 atcgcacgcg gcggtggcgt catacccagc tggtgagaca tgaagctttc aacggtattg
    17281 gtgattgcca cgctataaac cagcaggatc gggtagatag cgaagaagta gagcagggta
    17341 atcagtttac ctgcgccaat accaaagtgt tcttctacaa cctcggtgat gtcttcgccc
    17401 gggtttttac cagacagtac gaagcgagtc aggccgcggt gagcaaaaaa cgtcatcggg
    17461 aacgcaagga tagccatgat gatcagcggg atcataccgc caacaccggc gttgattggc
    17521 aggaacagca cgcccgcgcc gattgccgtg ccgtaaaggc ccagcatcca catggtgtct
    17581 gtcttgcgcc aggcactacg agagtctttc gacgcaatcg tgctggtttg agtcgtttcc
    17641 atctatttct cctggaggaa tgcaaataat tcagctttta gccagttcag cccgggaagg
    17701 tgcctgaccg taaaaatgaa atctgtctat cggcgatggc ttaatcattt accgccgtaa
    17761 taatttgagg gcggaaagat acacacaagt tatgtatcta tcagtgatct tgatcccaaa
    17821 tacaataatc tgcttggaag atagattatc ccaacgcttt actctgtaaa tcaaaagtaa
    17881 aagagaattt aagggcaata tttgcccatt aaacgacatc tgacttcttc cggggcgcgc
    17941 aatatatgta ggttgaactg aaaaaatcaa tacttttagt taatcatggc gataatttct
    18001 ttttcatagc gataaaatac ggtttatgaa aatagggaaa cgtttgtgtt gcaacataaa
    18061 ttgtatgaca tattaactgt gatgatattt gtcgcgtgag ggtggtaaga aacattcata
    18121 taacaaatga aatgagttga tgagtaagtg accaaaaacg agaaaacccg gcaacaa
//
LOCUS       DAZXUK010000056        18105 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZXUK010000056 DAZXUK010000000
VERSION     DAZXUK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18105
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.056"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1354
                     /locus_tag="ACH3L8_001563"
                     /pseudo
     CDS             1..1354
                     /locus_tag="ACH3L8_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            1364..3682
                     /gene="yicI"
                     /locus_tag="ACH3L8_001564"
     CDS             1364..3682
                     /gene="yicI"
                     /locus_tag="ACH3L8_001564"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFT4084733.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(3735..5444)
                     /locus_tag="ACH3L8_001565"
     CDS             complement(3735..5444)
                     /locus_tag="ACH3L8_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFT4084734.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(5565..6956)
                     /gene="xanP"
                     /locus_tag="ACH3L8_001566"
     CDS             complement(5565..6956)
                     /gene="xanP"
                     /locus_tag="ACH3L8_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFT4084735.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            7236..8441
                     /gene="gltS"
                     /locus_tag="ACH3L8_001567"
     CDS             7236..8441
                     /gene="gltS"
                     /locus_tag="ACH3L8_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFT4084736.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(8475..10556)
                     /gene="recG"
                     /locus_tag="ACH3L8_001568"
     CDS             complement(8475..10556)
                     /gene="recG"
                     /locus_tag="ACH3L8_001568"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFT4084737.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(10562..11251)
                     /gene="trmH"
                     /locus_tag="ACH3L8_001569"
     CDS             complement(10562..11251)
                     /gene="trmH"
                     /locus_tag="ACH3L8_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFT4084738.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(11258..13366)
                     /gene="spoT"
                     /locus_tag="ACH3L8_001570"
     CDS             complement(11258..13366)
                     /gene="spoT"
                     /locus_tag="ACH3L8_001570"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFT4084739.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMGVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(13385..13660)
                     /gene="rpoZ"
                     /locus_tag="ACH3L8_001571"
     CDS             complement(13385..13660)
                     /gene="rpoZ"
                     /locus_tag="ACH3L8_001571"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFT4084740.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            complement(13715..14338)
                     /gene="gmk"
                     /locus_tag="ACH3L8_001572"
     CDS             complement(13715..14338)
                     /gene="gmk"
                     /locus_tag="ACH3L8_001572"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFT4084741.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            14478..14735
                     /locus_tag="ACH3L8_001573"
     CDS             14478..14735
                     /locus_tag="ACH3L8_001573"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084742.1"
                     /translation="MIYCNKDDSQEASSQTPFLPLGFAFTTLYICQPDYSGGEDESMD
                     GDINKYLVLAIICVGGLSGLVASQSTGRNFPPATANKTVGR"
     gene            14647..16278
                     /gene="ligB"
                     /locus_tag="ACH3L8_001574"
     CDS             14647..16278
                     /gene="ligB"
                     /locus_tag="ACH3L8_001574"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFT4084743.1"
                     /translation="MWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVEDGVY
                     DQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRKMADKNALSLWMRERSDL
                     WVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSLISAVLQTVSGPLANSTL
                     QGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSLAVFVWAWPDGPQLMTDR
                     LKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRGAKEPESRHWL
                     PGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLAPVMLDDKKVQRVNIGSV
                     RRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNPLTCYFASDVC
                     QEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAK
                     SKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPG
                     TGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(16275..16892)
                     /locus_tag="ACH3L8_001575"
     CDS             complement(16275..16892)
                     /locus_tag="ACH3L8_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFT4084744.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(17183..18007)
                     /gene="dinD"
                     /locus_tag="ACH3L8_001576"
     CDS             complement(17183..18007)
                     /gene="dinD"
                     /locus_tag="ACH3L8_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFT4084745.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
BASE COUNT         4089 a         4599 c         4908 g         4509 t
ORIGIN      
        1 aaaattggtt atggcatggg agacgccgcc agccacatta ttttcgataa cgtaatgtta
       61 tatatgatgt tcttttatac cgatattttt ggcattcctg ccggatttgt cggaaccatg
      121 tttttggtcg ctcgtgcact ggatgcgatt tccgatcctt gcatggggtt gttggccgat
      181 cgaacgcgct ctcgctgggg taaatttcgt ccgtgggtac tgtttggcgc actgccattc
      241 gggatcgtct gtgtactggc ctatagcacg ccagatctca gtatgaacgg caaaatgatc
      301 tatgcagcaa ttacttacac cctaacttac cttactttat accgtcgtca atatccctta
      361 ctgcgcattg ggtggtgtaa tcaccaatga cccgactcag cgtatctcgc tgcaatcctg
      421 gcgttttgtg ctggcgaccg cgggaggcat gctttctact gttctgatga tgccactggt
      481 taatttaatt ggcggtgata ataaaccact cggtttccag ggcggtatcg tggtcctttc
      541 cgtggtggca ttcatgatgc tggcattttg tttcttcacc actaaagaac gcgttgaagc
      601 accacctaca acaacgtcta tgcgggaaga tttacgtgat atctggcaaa acgaccagtg
      661 gcggattgtc ggtttactaa ccattttcaa tatcctggcg gtgtgcgtac gcggtggggc
      721 gatgatgtat tacgtcacat ggattttggg cacgccggaa gtgtttgtcg cttttctcac
      781 cacttattgc gtgggtaacc tgattggttc cgcactggca aaacctctga ccgactggaa
      841 atgtaaagtc actatcttct ggtggacgaa cgccctgctg gcagtgatta gcctcgcgat
      901 gttctttgtt cccatgcagg ccagcatcac tatgtttgtc ttcatcttcg tgattggtgt
      961 gttgcatcaa ctggtgacac ctatccagtg ggtaatgatg ttcgataccg tcgactacgg
     1021 cgagtggtgc aatggtaaac gcctgaccgg gatcagtttt gctggcacgc tgtttgtgct
     1081 caaactgggg ttggccttcg gcggcgctct tatcggctgg atgctggctt atggcggata
     1141 tgatgcggca gaaaaagcgc agaacagcgc cacgattagc atcattattg cgctattcac
     1201 gattgttccg gcgatctgtt atttgctgag cgcgattatc gctaaacgct actactcact
     1261 cacgacgcac aatctgaaaa ccgttatgga acagctggct cagggtaaac gccgttgcca
     1321 gcaacaattc acctctcaag aagtgcagaa ctaaggaacg gcaatgaaaa ttagcgatgg
     1381 aaactggttg attcaacctg gcctcaattt gattcacccg cttcaggtat tcgaggttga
     1441 acagcaggat aatgaaatgg tggtctatgc tgccccccgt gatgtgcgtg aacgtacctg
     1501 gcagcttgat acgcctttat ttacgctgcg ctttttctcc ccacaggaag gtattgtcgg
     1561 tgtgcggatt gagcattttc agggggcgct gaataacggt cctcattatc cgctcaatat
     1621 tttgcaggac gtgaaggtca caatcgaaaa cacagaacgt tatgctgagt ttaaaagtgg
     1681 caacttaagc gcgcgtgtca gcaaaggtga gttctggtca ctggattttc tgcgcaacgg
     1741 cgaacgtatt accggtagtc aggtgaaaaa taatggctac gtgcaggaca cgaataatca
     1801 acgcaattat atgtttgagc ggcttgatct tggcgttggc gaaacagttt acggtctggg
     1861 agagcgcttt actgccctgg tgcgcaatgg ccagacggta gagacctgga accgggacgg
     1921 cggcacaagt actgaacagg cgtataaaaa tatcccgttc tacatgacta accgtggtta
     1981 tggggtactg gtcaatcatc cccagtgtgt ctcttttgaa gtgggatcgg agaaagtctc
     2041 caaagtgcag ttcagcgttg agagtgaata tctcgaatac tttgttatcg acggcccgac
     2101 gccgaaagcg gtacttgatc gttatacccg ctttactggt cgtccggcgc tgccgccccc
     2161 gtggtccttc ggcctgtggc taaccacttc atttaccacc aactacgacg aagcgacggt
     2221 aaacagcttt atcgatggta tggcggaacg caatctgccg ctgcatgttt tccactttga
     2281 ctgtttctgg atgaaagcct tccagtggtg cgattttgag tgggacccgc tgactttccc
     2341 tgacccggaa gggatgatcc gccgcctgaa agcgaaaggg ctgaaaatct gcgtctggat
     2401 taacccctat atcggtcaaa aatcccccgt ctttaaagag ttacaagaga aaggctattt
     2461 actcaaacgc ccggacggtt cgctatggca gtgggataaa tggcagccag gtctggcgat
     2521 ttatgacttt accaatccgg atgcctgcaa atggtacgcc gacaaactga aaggtctggt
     2581 cgcgatgggc gttgattgct ttaagaccga ctttggcgaa cgtatcccaa ttgatgttca
     2641 gtggtttgac ggttccgatt cgcagaaaat gcataaccat tatgcgtaca tctacaacga
     2701 actggtgtgg aacgtgctca aggacaccgt tggtgaggaa gaagctgtct tgtttgcccg
     2761 ctcggcctcc gtcggtgcgc agaaattccc ggtacactgg ggtggcgatt gttacgctaa
     2821 ctacgaatca atggcggaaa gcctgcgcgg tggtttgtct attggccttt caggttttgg
     2881 cttctggagc cacgatatcg gcggctttga aaataccgct ccggcgcacg tttacaaacg
     2941 ctggtgcgcg tttggtttgc tctccagcca tagccgttta cacggtagca aatcttatcg
     3001 tgtgccgtgg gcctacgatg atgagtcctg tgatgtggtg cgcttcttca cgcaactgaa
     3061 atgccgcatg atgccgtatc tgtatcgtga agctgcgcgt gcgaacgcgc ggggtacgcc
     3121 gatgatgcgg gccatgatga tggagttccc ggacgatccg gcttgtgatt accttgaccg
     3181 tcaatacatg ttaggcgaca acgtgatggt tgcgccggtg ttcactgaag cgggcgatgt
     3241 gcagttctac ctgccggaag gtcgctggac acacctgtgg cacaacgatg aactcgacgg
     3301 tagtcgctgg cataaacagc agcacggctt cctgagtctg cccgtttatg tgcgtgataa
     3361 cactctactg gcgctgggca acaacgatca acgtcccgat tacgtgtggc acgaaggcac
     3421 ggcattccac ctctttaatc tgcaagacgg gcatgaagcc gtctgtgaag tgcctgctgc
     3481 tgacggatcg gtgatcttta ctttaaaagc agcacgtact ggcaacacga ttactgtgac
     3541 tggtgcgggc gaggcgaaga actggacgct gtgcttgcgc aatgttgtga aagtaaatgg
     3601 tctgcaaggc ggttcgcagg ctgaaagtga gcaggggctg gtggtgacgc ctcaagggaa
     3661 tgcgctgaca attacgttgt aatctattga cggaatgccg ggtacgtctg agtcgtaccc
     3721 ggcgagattg atgtttaccg caccggctcc gtcgatgtaa cttcaccgct ggaaacgacg
     3781 cccgcattca tggtttgagt cacttgctgc tttgcggcgt tcacggcatg caattgcccg
     3841 ctaaccgtag gtttcagcgg ggcattggcc tgaatatctc cactggcggt aagctgaata
     3901 ttgccgtcgc cagtcaacgg taattcaggc catccccatt gttgcaaaat attcaccggc
     3961 acaccgcgtc cattcaggct gatatgggtc tgacgttgtg gcgtttgtga aacacttgcg
     4021 gtggcttcca gaatgccttt ttcagtaaat gcactcagtt cgctgatatt caccgtgctg
     4081 ctgttggcgg tcagcgccag cgacgggcga cgaacatcaa cacgattgaa tgtcgcggcg
     4141 gcggcattca gattcgccga gccactccag acgccccatt tatgatcggt aaccagcgtc
     4201 aggttggcac cgtaaccatc gagcgcggtg agctgccacg ggaagtcagg gtcgatatca
     4261 atgatcagat tgcggctggc gctaaatctc ttcagttgca ggctgtttaa ccaaccgggt
     4321 gtcgtttcca tccacaactg ttgccagttt ttcggcaagg tatattccag cccggcaatt
     4381 gccgcatcat caaggatcaa cgttttaccg tcacgcagcc agttccctga cgttctgacc
     4441 ataccccctt cccagcggct ggtgaactgg cgcagcgcta cgccctgcgg ggaaaattcc
     4501 gcgtttataa tcgggtcaaa taaatgcagc gaaccataaa tgaactcgct ggcgttcatc
     4561 gacagtttgc cttcctgtgt ctgccagtca tctttactga aggtcatgtt gcgcaagctg
     4621 agatcgaggt cggtcaccgc ccagtccgga ccttgcagac gagcatcgat gacttccaag
     4681 cgaccaatct gcaacgacgg gacagagcgt aatggcgcaa agaagtcggt tagcgatttt
     4741 tcgctttgta gacggatatc cgccatgcgc agattttcca cttgccagct gccgtcggcg
     4801 ttacgctgcg cgtttccggt taatgtcccg cgggcgatgt cggcacccag gttagtcagc
     4861 gtaacgcgat cgttatcaat actgccttca atcagtacat tggtggcagg aacatcattg
     4921 agcgaaagcg atccggcact aaactgaatc tgcgccttcg tacccagcac tttaccggct
     4981 tctggtgacc acggaaccac gccgccattt acccgctgcg cgctcagttt ccattcgcta
     5041 ttcgggctat taaacgccat atcacgcagt tgcagacgat cggctttgaa cggtagcggc
     5101 gcggtctggt cggtgagatt cagcgtcccg ttttccagca ggatggtatc gacatggcgt
     5161 ggttcggtca gttggcgact gcttagcgca atgtcgacac tttttgccac caggggcgcg
     5221 ggctgaccat cacgaccaaa cgtgacattc tccagcacga tatgagatgg cgcggaaaaa
     5281 cggtgatcca tcgccccgaa ggccagatga tagtcgctat tctcggaaac ccatgcgctg
     5341 atatgttctg ctccccagcg ggtttgcaga agaaaataga ggccagcgat caccactaac
     5401 agagcgatga gaatgtagag aagcagcttc ccaataaatt tcatggtctt ccatcccgtg
     5461 aaatgcacat aagggagtta tgcacgattt acgcgcaatc ctcaaggcgg gaatggtgaa
     5521 agaggtgaga ctgcggcggt aattatctta ccgccgccag tgaattactg tttttctggt
     5581 gggaaaatca gattcagcac gatggcagta ataccgcccg cggcgatccc ggaggagagc
     5641 aggtttttca gccattcagg ggcaaactgc aaaatcagcg gctgctgaga cacgcccaga
     5701 ccaaccgcca gcgacagcgc gataatcaga atcgcccgac ggttcagcgg ctcacgagaa
     5761 acgatacgca caccggaggc ggcgatggtg ccaaacatta caagcgttgc gccgcccaga
     5821 accggttctg gaatgtgttg tacaaaaccg ctcactgccg ggaacagacc cagcacgatc
     5881 aacatcagcg cgacgacaaa accgacatag cggctggcaa caccagtcaa ctggatcact
     5941 ccgttgtttt gcccgaagca ggagttcggg aaggtgttaa acaccgccga aacaaacgag
     6001 ttcaggccgt ttgccagcac gccgcctttc aggcgtttca tgtacagcgg accggacact
     6061 ggctgttcgg aaacgtcaga ggtcgccgtg atatcgccaa tggtttccag cgaagtgatc
     6121 ataaagacca gcatcagcgg cagcagcaga ctccattcaa tgccaagacc gtaatagagc
     6181 ggcgttggca ccataatcag ttcttgcgtc atcggttcgt tgctttctgg caacatgccc
     6241 ataaaccacg ccagcgcata tccggccgcc atcgcaatta ccagtgaggc cacgcgtaag
     6301 taagggttac gttgacggtt aagcaggata attaaggcta aaaccacgcc tgccagcagc
     6361 agatttttcg gtgcgccgaa ggtgttatcg ctcatggctg cgtaaccgcc gccaatggag
     6421 gtcagcccca cctgaattag cgacaggccg ataatcatca ccacaacgcc agaaaccagc
     6481 ggcgtaataa tgcggcgcgc cagatgcaga acgcgggaga tcaccatctc ggtgcaactt
     6541 gccagcatca acgtgccgaa caaagccgcc atcatggtag gaacatcagc accaccggtt
     6601 ttcagcgcgg taccgcccat aatcagcggg gcaacaaagt tgaagctggt gccctgaata
     6661 gacaacagcc cggagccaac cggaccccag gccttaattt gaataatcga tgccacaccg
     6721 gaggcaaaca gcgacatact aataatgtgt tgcgtgtctt gtgccggtaa acccagcgcc
     6781 tggcagatta atagcgctgg cgtgatcacc gcaacgaaca tcgccagcag atgctgacag
     6841 gcggcaaaca gggtttgagg aagcggcgga cgatcttcaa gacggtaaat cagttcgctg
     6901 ttttgagtct gagcaaccgg ttgcgcattt tctgactcga gggtggaaac agacatcgaa
     6961 aacaatcccg tggtagcaaa gcgggcattt tagctgaccg aaagagaaaa gcaaacgttt
     7021 gccacatcct catctaaata tttaaatgag ataaatgcac ttttttatat aacttttggt
     7081 atttttctgc ttaaaatcca tgccatttgt tcattaacag ctgagaattt gacccaggaa
     7141 agcataactc gatacccgtc atagttcatg aagttgccac tgcgcgatga agtatgacga
     7201 gtatgaaaga gtgatgcgga tacaaaggag taactatgtt tcatctcgat actttagcaa
     7261 cgcttgttgc cgcaacgctg acgttgctgc tcgggcgtaa gttggtccat tccgtctcct
     7321 ttttgaagaa atacaccata ccggaacctg ttgcgggtgg tttgttggtg gcgctggcgc
     7381 tactagtact gaaaaaaagc atgggctggg aagtcaactt tgatatgtcc ctgcgcgatc
     7441 cgttaatgct ggctttcttc gccaccattg gcctgaacgc caacattgcc agtttgcgtg
     7501 ccggtgggcg tgtggttggc atcttcttga ttgtggttgt tggtctgttg gtgatgcaaa
     7561 atgccattgg cattggtatg gctagcctgt tagggcttga tccgctgatg gggctgttgg
     7621 ccggttctat tacgctttcc ggcggtcacg gtacgggcgc tgcgtggagt aaattgttca
     7681 ttgaacgtta tggcttcacc aatgcgacgg aagtggcgat ggcctgtgca acgttcggtc
     7741 tggtgctggg cggcttgatt ggcggtccgg tggcgcgcta tctggtgaaa cactccacca
     7801 cgccgaacgg tattccggat gaccaggaag tcccgacggc gtttgaaaag ccggatgtgg
     7861 gacgcatgat cacctcgttg gtgctgattg aaactatcgc gctgattgct atctgcctga
     7921 cggtggggaa aattgttgcg caacttttgg ctggcactgc ttttgaactg ccgaccttcg
     7981 tctgtgtact gtttgttggc gtgattctga gcaacggtct gtcaataatg ggcttttacc
     8041 gcgtctttga gcgtgcggta tccgtgctgg gtaacgtaag cttgtcgttg ttcctggcga
     8101 tggcgttgat ggggctgaaa ctgtgggagc tggcttcgct ggcgctgccg atgctggcga
     8161 ttctggtggt acagaccatc ttcatggcgt tgtatgccat cttcgttacc tggcgcatga
     8221 tgggcaaaaa ctacgatgcg gcagtgctgg ctgcgggtca ctgtggtttt ggcctcggtg
     8281 caacgccaac ggcaatcgcc aacatgcagg cgatcactga acgctttggc ccgtcgcaca
     8341 tggcgttttt ggtggtgccg atggtcggtg cgttctttat cgatatcgtc aatgcgctgg
     8401 tgattaagct gtatttaatg ttgccgattt ttgccggtta accgatgaag cggcggtctt
     8461 ctcaccgccg ccttttacgc attcgagtaa cgttctgtct ccggcatcca gcgttctatc
     8521 aaggcttttg cctgttgtgg gtaacgttcg tgaatatgtc gcgccaggcg ctgaacttcc
     8581 gggatcatcg cctgatcgcg cagtaaatcc gccactttaa attcagcatt acccgtctga
     8641 cgcgtgccta acaattcgcc agggccgcga atctccagat ctttttgcgc aatcacaaaa
     8701 ccgtcgttac tgtcgcgcag cacttgcagg cgaatttgcg ccgttttaga aagcggcgtt
     8761 ttgtagagca gcacgcagtg agaagccacc gcgccacgac ctacgcgccc gcgcagctgg
     8821 tgtaactgcg ccagacccag acgctcaggg ttttcgataa tcatcaggct ggcgttaggc
     8881 acatcaacac cgacttcaat aacggtagtg gcaaccagca gatgtagctc accttgttta
     8941 aacgacgcca tcaccgcctg tttctcggct ggtttcatcc gcccgtgtac caggccaaca
     9001 ttcaattctg gtagcgccag tttcaactct tcccaggtag cttccgccgc ctgcgcttcc
     9061 agtaactctg actcttcaat caacgtacaa acccagtacg cctgacgacc ttcagttatg
     9121 caggcgtggc gcacgcggtc aatgatgtcg gtacggcggg tatcaggaat agcgaccgta
     9181 gtcactggcg tgcggcctgg cggcagctca tctatcaccg aggtatcgag atcggcatac
     9241 gccgtcattg ccagcgtgcg ggggatcggc gtggcggtca tgatcaactg atgcggatgg
     9301 aagccctgct gctggccttt ctcccacaat gccagacgct gatgcacgcc aaaacgatgc
     9361 tgttcgtcga taatcaccag cgccaggccg ttaaactgca cctgttcctg gaagatggcg
     9421 tgtgtaccga caatcatctg cacctgaccg ctggcgatgg cttcctgctg agacagtcgc
     9481 gctttgcctt tctgcttacc ggcgagccag cccacttcga taccgagcgg tgcaaaccag
     9541 ttgcggaagt tattggcgtg ctgctcggcg agtaattcgg ttggtgccat cagtgccacc
     9601 tgtttgccgt gggcaatcgc acgcagcgcg gcgagggcgg cgaccagcgt tttaccggaa
     9661 cctacatcgc cctgtaccag gcgcatcatc ggcacatcca gcgccatatc atgctcgatc
     9721 tccgccacta cgcgtgcctg tgcgcccgtt ggcttgaacg gtaaggcggc gaggagttta
     9781 tttttcagcg tgtcattggc gctcagcggc tgggcatgaa aacgctgtgc tccggcacgt
     9841 aaggctaaca tgctgaggtt gtgcgccagc agttcttcca gaatcagacg acgttgcgcc
     9901 ggatgctgcc cggtttccag atcgctaagc tgtagcgtcg gtggcgggcg gtgcaaagtg
     9961 cgcaacgctt ccggtagcgt catcattcct tgtgacagtt ccggcggcag gagttcttca
    10021 atggcgcagg tgtcgagcag atccagcgcc tggtcggtta atttacgcag cgtggcctgc
    10081 tttacgcctt ccgttgttgg ataaaccggc gtgagcgttt cctgtaattc tggcgtgctg
    10141 agatcgccct gcacgcggta ttccgggtgg atcatctccg caccatattt accgcgcttt
    10201 gcttcgccat aagccagtac acggcggccc gtcgccaggc tatttttcat tgccgcgctg
    10261 aaattgaaaa agcgcatggt gaggatgccg gaaccgtcgc tgatctggca ggtcatcatc
    10321 cgccgaccgc cgaaggagat attgcagttc agcacttcgc cttccaccgt ggcataaacg
    10381 cccggcagta gttctccgat ggggtagaga tgggtgcgat cttcgtagcg caggggaagg
    10441 tgtaagagta gatcctgcac ggtatgcagg ttgattttcg ccagtttgtt actaagtgct
    10501 gcgccaacgc ccgttaggga actgagtggg acagcatcta acaggcgacc tttcatggca
    10561 cttaccctgc agcctgcata gtagcccacc agtcggcatc agcttcgatc tcgccttgct
    10621 gattgacgtg gggataaggc aggcctttgc gttttgcgac tttcgccagc accggatagc
    10681 cgccttcaaa caacaggcgt tgttgctctg cttccggcaa catgctgttt tcacgcaggt
    10741 acatgcctgc attttgccgc tgacgctggg cttcgtaaag aatgagggct gaggcaacgg
    10801 aaacattcag cgactgcacc atgccgatca tcggaatgat gatgtcctga tccgccaggg
    10861 ccaatgcttc ctgcgtgatg cccgttttct cctgtcccat caaaatgcag gtcgggcgag
    10921 tgtaatcaat ttcgcggaaa tcgacagcat tatcagaaag atgggttgcc agaatctgca
    10981 tgccctggcc tttgagatga gcgacggcat cgccaatggt gcggtgtgtt ttcacctgta
    11041 cccagctgtt actacccgcc gctgccgaag ccatggtgcg catgcggcta ccaggccaga
    11101 cggcgtgaac ttcatgtacg ccaacggcat ctgcggtacg aataatcgca gaaacgttat
    11161 gaggtttgtg gacctgctcc atgcagacgg tcagatcagg ctgccgcctg gcgagcattt
    11221 cgcagatgcg tgcataacgt gttgggttca taaaacatta atttcggttt cgggtgactt
    11281 taatcacgtc tggcatcacg cggattttgc gcatgatatt cgccagatgc acacggtcac
    11341 gagcggtcag acgaataaag gcgctgtaga cgcgaccatc tttctcttcc gtattcaaac
    11401 tttgaatatt cgaagtcgtg gtgttaattg ccgccgtcag gtttgccagc gcgccctgat
    11461 gattgaacat ctccaccttg atttcggtga tgaactcctg cgccgtctct ttatcccatt
    11521 ccacacccat aaacttctct ggctctttct ggtagccacg gatattacgg caggattcat
    11581 ggtggatcac cagaccttta ccggggctga cgtgggcgat aatcgggtcg ccaggaatag
    11641 ggcggcagca tttcgcaaag gtgatcagta cgccatcggc acctttaatg ggcagatgtc
    11701 cgtggctttg ggttgccggt ggaatggagg cgtccccatg ttgcagattt ttcgcgacca
    11761 ccacgctcat tgcgttacca agtccgattt ctgccagcag atcgtcaagc gttgccagct
    11821 tcatgcgatc cagctcgcgc tgaatatttt cctgcgggat ttcattcagc ttacggctac
    11881 cacccaaagc atggttgagc agacgacggc ccaggcttac agaatcatca cgcttgaggt
    11941 ttttcagcaa ctgacgaatt ttggcgcgcg cttttgagct aacgacaaag ttcagccacg
    12001 cggcattcgg gcgagcgccc ggtgcggtaa tgatttcaac ggtttgaccg ctggtaagcg
    12061 gctgcgacag cgggtaaggc tggcggtcaa cgcgtgcgcc cacgcaggca tgaccgatat
    12121 cggtatgcac tgcataagcg aagtcgacgg gcgttgcacc ggcaggcagc tcgacaattc
    12181 gcccttccgg tgtgaaaacg taaatctcat ccgggaagag atcggattta acgctctcga
    12241 taaattcaaa cgaactaccg gcgctctgtt gcagctccag caggctttgc atccagcgct
    12301 gggcgcggat ttgcgcggtg gtgctggttt cgccgtgctc tttataagcc cagtgcgcgg
    12361 caacacccat ctccgccatc tgatccatat cttcggtacg gatctggacc tcaaccggca
    12421 caccgtgcgg gccgatcatc gaggtgtgca aagactgata gccgttcgct tttggaatgg
    12481 cgatgtagtc tttcacgcga cccggacgcg gcttgtacag gctatgcatt tgcccgagca
    12541 cgcgataaca ggtgtcggaa tcattgacga tcacgcggaa agcgtagatg tccatgattg
    12601 agtgaaaacg ctgctctttg agcaccattt tgcagtaaat cgaataaaga tgcttctcgc
    12661 gaccactgac gcggcaggga attcccgctt cctgcaaacg cccttcgatt tccgagagga
    12721 ttttttggat catctcttta cggttgccgc gcgcggcttt caccacttct ttgattacgc
    12781 gataacggtt gggatacagc gcctcaaaac ccagctcttc gagttcggtt ttaatgtggt
    12841 ggatacctaa acggtgcgcc agcgggctgt aaatttcgag agtttcacgg gcgatgcggc
    12901 gacgtttgtc cgggcgaagt gagcccagcg tgcgcatgtt gtgggtacgg tcggcaagtt
    12961 tgatgaggat gacgcggata tcctgcacca tcgccataat catcttgcga aagttttcgg
    13021 cctgcgcctc tttcttatcg cggaacttga gtttatcaag tttcgacacc ccctctacca
    13081 gctcggcgac gcttttacca aaaagctgtt ccatatcctg gtaggtggcg ggagtatctt
    13141 caatcacgtc atgcagcagc gccgccatca gcgtttcata gtcgagtttc atctcggcca
    13201 gaatgcaggc aaccgctacc gggtgcgtga tatagggttc accgcttgaa cgtgtttgcc
    13261 cctcgtgagc atcacgtgca acgagatacg cctgccgcag acgcttgatt tggtcttccg
    13321 gcaggtaggt ttgaatcagt tgattcaggc tttcaaacag atacaagggc gacccgcttt
    13381 gtgattaacg acgaccttca gcaatagcgg taacggcttg taattcagcg gcttccagct
    13441 cttgctgttc ctggcgttcg cgaacgtcga ggatctggtt gttgatcaga ccttcttcga
    13501 tttcgcgcag cgcgattaca gtggttttat cgttttcttc cggtaccagc ggatcctttc
    13561 cgcctacctg catctgacga gcgcgacgcg cggcgaccag taccaggtca aaacggttac
    13621 caattttctc tacagcgtcc tgaacagtta cgcgtgccat acttaaaaag ctccacaggt
    13681 gaagaaatga ctgggcatga tactgaaatc aggttcagtc tgccaacaat ttgctgatta
    13741 aagcgtcatg acgctgcttt tggcggctca tgcgcagacg ttcggcgcga ataatggtct
    13801 tcaaatcggt caacgcggta tcgaagtcat cattcacaat cagataatca tattcggcgt
    13861 aatggctcat ttctgcaaca gcttgcgcca tacgctttgc aatgacctct tcgctgtcct
    13921 gaccgcgacc gcgtagacgg cggtccagtt caattttgga cggcggtaaa ataaagatac
    13981 tccgcgcgtg cggcatcttc tggcgaattt gctgcgcgcc ctgccagtcg atatcgagaa
    14041 aaacatcgac accggtcgcc agtacttgct caatggcctc acgcgaagtg ccatagtaat
    14101 taccaaaaac ttctgcgtgt tcgaggaacg catctctgct aatcatttct ttaaattcat
    14161 catgattaac aaagaaataa tgttcaccgt ggacttcacc aggacgcggt tggcgtgtgg
    14221 tgtgtgaaac agaaacctgg gtgtcataca acggttgggt ttttaataaa gcctgaatca
    14281 ggctggattt acccgcgcca ctgggggcag aaacaatata aagcgtgcct tgagccatga
    14341 gtatctttcg tatgtgatta gcgaaataaa gcctacatac gagcttatta tacacggcgc
    14401 ggccatgtga cgtagccctt gctcacactt tttgcgcaaa gttgttgcgt tttgcgtagc
    14461 tgtttccgca tttgcctgtg atttactgca ataaagacga ttctcaggaa gcgtcctcgc
    14521 agactccgtt tttgccactc ggctttgcct tcaccacgtt gtatatctgc cagcctgact
    14581 acagcggagg tgaagatgaa agtatggatg gcgatattaa taagtatctt gtgctggcaa
    14641 tcatctgtgt gggcggtctg tccggcctgg tcgccagcca gagcacagga agaaatttcc
    14701 cgcctgcaac agcaaataaa acagtgggac gatgactact ggaaggaagg aaaaagtgag
    14761 gtggaagacg gtgtttacga tcagttaagc gcccgtctta cgcagtggca acgctgcttt
    14821 gtgagcgagc cccgcgacgt gatgatgccg cctttaaatg gtgcggttat gcatccggtt
    14881 gcccatacgg gtgtacgcaa aatggcggat aaaaacgcat taagtctgtg gatgcgagaa
    14941 cgtagcgatc tttgggtgca gccaaaagtt gatggcgttg cggtaaccct ggtttatcgg
    15001 gacgggaaac tgaacaaagc aatcagtcgc ggtaatggcc tgaaaggcga ggactggacg
    15061 cagaaagtta gcttaatttc cgctgtgctg caaaccgtta gcgggccttt agccaacagt
    15121 acgcttcagg gggaaatatt tctccagcgc gaggggcata tccaacaaca aatgggggga
    15181 ataaatgccc gcgcaaaagt tgctggcctg atgatgcgcc aggggaatag cgacacgcta
    15241 aactcactgg ctgtttttgt ctgggcatgg ccggatggac cgcagttaat gaccgatcgt
    15301 ttaaaggagc tggctaccgc aggttttact ctaacgcaga ggtatacccg tgcggttaaa
    15361 aatgctgatg aggttgcgcg cgtacgcaat gagtggtgga aagcgaaatt acccttcgtc
    15421 accgatggcg tggttgtgcg aggggctaag gaacccgaat cccgtcattg gctaccgggc
    15481 caggcggagt ggctggtggc ctggaaatat caacctgtag ctcaggttgc cgaagtgaag
    15541 gcgattcagt ttgcggtggg taagagcggt aaaatatcgg tggttgcgtc actcgcacct
    15601 gtcatgctgg atgataaaaa agtccagcgg gtgaatattg gttccgtcag gcgctggcag
    15661 gagtgggata ttgcgcctgg tgatcagatc ctcgtcagcc ttgccggtca gggtattcct
    15721 cgcattgatg atgtggtctg gcgcggtgca gaacgtacaa aaccgacacc accagaaaac
    15781 cgctttaacc cgttgacctg ctactttgct tctgatgttt gtcaggaaca gttcatttca
    15841 cgcttagtct ggctggggtc aaaacaggtt ctggggctgg atggcattgg tgaggccggt
    15901 tggcgcgcgc tgcatcagac tcatcgcttt gagcatatct tttcctggct tttattaacg
    15961 ccagagcaat tacagaacac gccggggatc gcgaaaagta aaagtgcgca gttatggcat
    16021 cagtttaatc tggctcgtaa ccagcctttt actcgctggg tgatggcaat gggaataccg
    16081 ctaacccggg cggcgcttaa tgccagtgat gaacggtcct ggtcgcaact tttatttagc
    16141 acggagcagt tctggcagca gctgccgggg actggatctg ggcgcgccag acaggttatt
    16201 gaatggaagg aaaatgcgca aatcaagaag ctgggcagtt ggctggctgc ccagcagatc
    16261 acaggttttg aaccttagtg accttcgtgg ctgtagtaaa agaccggtaa tcccagctta
    16321 agacgtagcg ccagtaaacg agcaaaaaag ccgaatacca gggtggagat aatcaccaca
    16381 tcatggttag aaacatagtg ttgcagcgcg atgtacagca cggcggaggc aaatgagaca
    16441 ccggcataca actctttttg aaagaccagc ggaatgcgtt tacagaacat atcgcgcaaa
    16501 acgccgccaa atacaccggt ggttaccgcc gcgacaacgg caataattgg gccgtgcccc
    16561 atatccagtg caacctgtgc gccgatgata gaaaagacca ccagtccgag cgcatcgagc
    16621 accagaaaca ctttgcgcag gtaaggcatt acaggagcaa cgatagtggt gagtactgcg
    16681 gcggtagcga cgataatcac atattccggg tgtttgaccc agccgagcgg atagtggccc
    16741 agcagaatat cgcgcactga ccctccgcca attgcggtgg cggttgcaat aataattacg
    16801 ccaaatgtat ccatgcgccg tcgcccggcc gccagcgccc ccgtcatggc ttcggcagta
    16861 atgccaacca aatacaaaat gtgtaacagc atggttctct ccctaaaaat ctgtctggca
    16921 gggtagcgat ttgtgcgcat tgtcacgatt gatatttttt aaggtgagag gaaattgata
    16981 acttttagtt atgtgaaatc gctattttct gtagcgtaga tgcattcttc tgacttcttg
    17041 tctgagaaaa tgcataagaa aaaataggtt aaaactgagg ggggaaaaga aaggtgcaat
    17101 gtttagccat tgtgttcact gcacctggac cttggcttac gcatcgggtg tctcaatcaa
    17161 caaaaagagt ttttcgtttc tgctatttct tctctgtaat ttttacactg ttttccaact
    17221 gcttgatgct gacctgcggg gtcggcaact cctcaggtat ggttccgcca agttcctgaa
    17281 tggtttgcct caccttgcta cccacgtcaa agtgtgtggt attagcttgc tgttttgaat
    17341 tcacgctatc ccgcttgagt ttttcttctg tttgggtagc tcgaaagaga ttagccgcca
    17401 gttctgttga acccatatga tccaggatct tctgactctt tttcagcccc ttcagttggt
    17461 ggatggcttt ctgatctaat ccaccataca gcccctggta accatgattc tgaaatatgg
    17521 cgaaatcagt agctgtcgct accccagctt gctgtgctgc ctcaaccaat tgtttgttat
    17581 gttctttcaa ctcatttctc agaaacagac gcttttcgtc ttcacgaagt tgcttaaatg
    17641 cttcatcgtc agcaagctcc tgccgtcggg tctggatagt aaaataagtc tgccctgccg
    17701 caatgaccgg tttcgcgggg tcgccgtttt gtactaccag gtaacaggca tagcgggaga
    17761 gtacgatgtc ttttaatgct cttttggcat ttgagccaat tttgaccata tcgaggatct
    17821 cctcgaaatg gtcatcaatt gtgtgaccac tgttctcaca ggcctctttg gcgcgcgtta
    17881 atacaggtat aaagtgacga tactctgaat aacccagtag tttcccaagt tgtcttgctg
    17941 accattgttc tgctccgttt gcattaatca gttttatctc ttcaaaaggt tgatgatgtt
    18001 cgttcattgc ccactccatg tgatatcgat tgcaaagaaa gtaaatctgt aactgtattt
    18061 atatacagtt gtgagtatct cacgttctca gtgtgctggg caaaa
//
LOCUS       DAZXUK010000057        18010 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZXUK010000057 DAZXUK010000000
VERSION     DAZXUK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18010
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.057"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            90..680
                     /locus_tag="ACH3L8_001577"
     CDS             90..680
                     /locus_tag="ACH3L8_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFT4084746.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINGIC
                     EAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(977..1798)
                     /locus_tag="ACH3L8_001578"
     CDS             complement(977..1798)
                     /locus_tag="ACH3L8_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFT4084747.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(1909..2205)
                     /locus_tag="ACH3L8_001579"
     CDS             complement(1909..2205)
                     /locus_tag="ACH3L8_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084748.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            2505..2801
                     /locus_tag="ACH3L8_001580"
                     /pseudo
     CDS             2505..2801
                     /locus_tag="ACH3L8_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3120..3245)
                     /locus_tag="ACH3L8_001581"
     CDS             complement(3120..3245)
                     /locus_tag="ACH3L8_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFT4084749.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
     gene            complement(3187..3336)
                     /gene="mok"
                     /locus_tag="ACH3L8_001582"
                     /pseudo
     CDS             complement(3187..3336)
                     /gene="mok"
                     /locus_tag="ACH3L8_001582"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            complement(3515..4277)
                     /locus_tag="ACH3L8_001583"
                     /pseudo
     CDS             complement(3515..4277)
                     /locus_tag="ACH3L8_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            complement(4274..4708)
                     /gene="psiB"
                     /locus_tag="ACH3L8_001584"
     CDS             complement(4274..4708)
                     /gene="psiB"
                     /locus_tag="ACH3L8_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFT4084750.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            complement(4763..6721)
                     /locus_tag="ACH3L8_001585"
     CDS             complement(4763..6721)
                     /locus_tag="ACH3L8_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFT4084751.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            complement(6785..7018)
                     /locus_tag="ACH3L8_001586"
     CDS             complement(6785..7018)
                     /locus_tag="ACH3L8_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFT4084752.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            complement(7074..7595)
                     /locus_tag="ACH3L8_001587"
     CDS             complement(7074..7595)
                     /locus_tag="ACH3L8_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFT4084753.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDKGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            7894..8337
                     /locus_tag="ACH3L8_001588"
                     /pseudo
     CDS             7894..8337
                     /locus_tag="ACH3L8_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8423..8986)
                     /locus_tag="ACH3L8_001589"
     CDS             complement(8423..8986)
                     /locus_tag="ACH3L8_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFT4084754.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREVLPCGTFAYTRVPTMIIRLRA"
     gene            complement(9033..10394)
                     /locus_tag="ACH3L8_001590"
     CDS             complement(9033..10394)
                     /locus_tag="ACH3L8_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFT4084755.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(10446..10676)
                     /locus_tag="ACH3L8_001591"
     CDS             complement(10446..10676)
                     /locus_tag="ACH3L8_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084756.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPTPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(10900..11019)
                     /locus_tag="ACH3L8_001592"
                     /pseudo
     CDS             complement(10900..11019)
                     /locus_tag="ACH3L8_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332787.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(11714..11905)
                     /locus_tag="ACH3L8_001593"
     CDS             complement(11714..11905)
                     /locus_tag="ACH3L8_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084757.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(11902..12324)
                     /locus_tag="ACH3L8_001594"
     CDS             complement(11902..12324)
                     /locus_tag="ACH3L8_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFT4084758.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(12371..12796)
                     /locus_tag="ACH3L8_001595"
     CDS             complement(12371..12796)
                     /locus_tag="ACH3L8_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFT4084759.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(13214..13990)
                     /locus_tag="ACH3L8_001596"
     CDS             complement(13214..13990)
                     /locus_tag="ACH3L8_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084760.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(14041..14475)
                     /locus_tag="ACH3L8_001597"
     CDS             complement(14041..14475)
                     /locus_tag="ACH3L8_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFT4084761.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(14489..14710)
                     /locus_tag="ACH3L8_001598"
     CDS             complement(14489..14710)
                     /locus_tag="ACH3L8_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084762.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(14711..15394)
                     /locus_tag="ACH3L8_001599"
     CDS             complement(14711..15394)
                     /locus_tag="ACH3L8_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFT4084763.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(15471..15764)
                     /locus_tag="ACH3L8_001600"
     CDS             complement(15471..15764)
                     /locus_tag="ACH3L8_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084764.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            15911..16873
                     /gene="parM"
                     /locus_tag="ACH3L8_001601"
                     /gene_synonym="stbA"
     CDS             15911..16873
                     /gene="parM"
                     /locus_tag="ACH3L8_001601"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFT4084765.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            16876..17226
                     /locus_tag="ACH3L8_001602"
     CDS             16876..17226
                     /locus_tag="ACH3L8_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFT4084766.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(17349..17630)
                     /locus_tag="ACH3L8_001603"
     CDS             complement(17349..17630)
                     /locus_tag="ACH3L8_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084767.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(17758..17992)
                     /locus_tag="ACH3L8_001604"
                     /pseudo
     CDS             complement(17758..17992)
                     /locus_tag="ACH3L8_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637348.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT         4147 a         5087 c         4626 g         4150 t
ORIGIN      
        1 tttaaacaat ataaatccac aaatacaact caatgatatt aaagataaat cagcaaaaac
       61 ttgtttttgc gtagtgtgtg gtgcttttgg tggtgagaac caccaacctg ttgagccttt
      121 ttgtggagtg ggttaaatta tttacggata aagtcaccag aggtggaaaa atgaaaaaat
      181 ggatgttagc aatctgcctg atgtttataa atgggatctg cgaagccgcc gattgctttg
      241 atcttgcagg tcgggattac aaaatagacc cggatttact gagagcgata tcatggaaag
      301 aatcccgtta ccgggttaat gccatcggta ttaatccggt aacgggatat ggcagcggac
      361 tgatgcaggt agattcccag cattttaacg aactggcccg ctatggaatt aagccggaac
      421 atctgacaac agatccctgt atgaacattt ataccggtgc ttattatctg gcaatagcct
      481 ttaaaaaatg gggcgtctcc tgggaggccg ttggtgcata caatgccgga ttcaggaaga
      541 ccgaacgcca gaaccagaga cgtcttgcct acgcatcaga ggtttaccgg atttatacct
      601 ggataaagag cagtaaaggc atccgggttc cgaccacgaa gaaatcactt tcccaaatta
      661 acagtgtgca gaaaaattaa tattgtatac attgtcaaat accggttatg ccgcgacagc
      721 ggcatggaag gccacacaca aaaatatcat atgagtgata atgctgactg gccgaagccc
      781 cgcaaagcgg gatcccatct tatatatatc cggtaactgc gcagcctcct gccagaccat
      841 ttttcgcggg cacaatattt acagactacc gaaaagccag ttgatgaaat tgaggcatat
      901 cagacgacac agcaatgccg caggatgata atataagtaa agccccgaaa aatttttcgg
      961 ggctttactt atattatcat gacttccact ccctgaactg ttcggcaatc atccataatg
     1021 ctttgttgat tcggatatca ccgtcaatgc ctgtaatggc tcttgttctg gtatttttac
     1081 cggaggcact tcgccccgat aatccacctt ttatcatatt ctcctgaacc cgctgccagg
     1141 ttgtccagag atcgttctgt ttatcctccc aacggcgggg agtaattatt tgttctggcg
     1201 tcactggcgt tttattttca tcttcgtacc tggccatcaa tgcagctctg ccaaataaat
     1261 gttgttcgtc actgttaaga tgtatctctt tcattgtctc catattttca gtgactttat
     1321 caaagacacc gagcacttca tacgctccct caataacctg cccgacaata tcacctttat
     1381 gtggaacgcg gatttcgcca aaattattcc cacacaccag gccatttgtg cagacaaaac
     1441 gaaaaattcc tgggatcatc tgataactgg atgaaccatc atgtgaatta agcaggataa
     1501 tttcaggaac ttctttcccg ttaatatgcc cttccctgcg aagacgtaac atatgtttac
     1561 tgtattcgcg gcgtcccaaa tcacgaaccc gactctgaca ggcaaagaat ggctggaaac
     1621 cttcatcacg taacttattg atgatattga tggttgggat atatgtataa cgctcactcc
     1681 gggattcatg tttatcacca gaaaagacag atggaacaaa ctgcattaat tcgtcatccg
     1741 ttaacgggcg ctcacggcga atagaattat atcgaccaaa acgggaagct aatcgcataa
     1801 cagtaactcc tttatgaagg cataaaatta ataaaagttt tttcgcttaa tatcatcccc
     1861 cctcggttat cgttaacaaa aagacaacaa ttccgagggg aaggaccatc aaatcaggtc
     1921 agttcgggac tgaccaacct gccgagtgaa aagcagtaac agcgcgatca actcatcagc
     1981 gttttttctg acaagaggag tatgattctc caggctatcc agtaagtcat gcagctcatt
     2041 attgacctgg tggagcacct taaagagtgc accagaatcc cggcagctct gatatgcaag
     2101 atttgcctga acagcacgat tctgcatgat cccgatttgt gcgattgcgt ctttctgctg
     2161 atcgcctgtt ggctgataag tggtgttaaa agaaaatgcg ctcataattc ctcctgtatc
     2221 tgatgttttc agcccccatc atctgttgag tttcgggagc cgcggttttg acgttttgcg
     2281 gggacctgcc ttttgttccc tccggtattc accatcacct gtttgagaac cttcgccgca
     2341 aggggcatca ctgccacggc ctgacgcacc tgtcctgggc agaacgcaaa attttttacc
     2401 gggttttggg taaaaaattt tgtgggctgt tcatttaccc tggacagtca gtgcggcagg
     2461 ttgtggctgt ctatgccccc ggcgacaggg gcttaaacag gtgaatggca ccggacagaa
     2521 caacgggcag ggacccgccg caacgacgtg aagcgcggtt cgcgaaacgg cgttgctcct
     2581 tacatcccgg cgtcttttgc cgggatgccg ggagcgcagg ccacggcgcg acaagggtgg
     2641 cgcgaccgtt ccggtcgctg caaacccggg tttttgcgtc agggatggaa gcccgccagg
     2701 gcggagacag cttcgctggc tccgttcacg acagcccgcc ccgccagggg gacgcccgga
     2761 aaaggcataa gcaggagaac aacaaaacag acaataatca gggcagcagt aatcagagtc
     2821 agagcagtaa tcagagtcag agcaggagga aaaagagaat aatcaaaggg aaggtgatgt
     2881 gatttctggt aagtcataaa cagagaaggc gggggaaaag aacgggcgcc gcaggcgcac
     2941 gttcatcccc gttcccggag ggaaacggcc ttcctggcag tctggttgtt catgcccgta
     3001 caatgccagc aaggagaaag gggccaccgg cgaaccagca gcccctttat aaaggcgctt
     3061 cagtgcagtc agaccagcat cagccctgaa aaggcgggcc tgcgcccgcc tccaggttgc
     3121 tacttaccgg attcgtaagc catgaaagcc gccacctccc tgtgtccgtc tctgtaacga
     3181 atctcgcaca gcgattttcg tgtcagataa gtgaatatca acagtgtgag acacacgatc
     3241 aacacacacc agacaaggga acttcgtggt agtttcatgg ccttcttctc cttgcgcaaa
     3301 gcgcggtaag aggctatcct gatgtggact agacataggg atgcctcgtg gtggttaatg
     3361 aaaattaact tactacgggg ctatcttctt tctgccacac aacacggtaa caaaccacct
     3421 tcacgtcatg aggcagaaag cctcaagcgc cgggcacatc atagcccata tccctgcacg
     3481 ctgaccacac tcacttttcc tgaaaataac ccgctcattc aggctgttta cgggtaatct
     3541 gtgtgattgt tgccgcatca cgccgcctcc cggagtttgt tcggcaccat ccaccgctcc
     3601 atctcacgga cagcgtgacc ggcctctctt gctgcaagac tggcttccgc aataacctgc
     3661 cagaacggca tatcctgcca ctgccagcgt tccattccgg ccagtgacgg aaaacgctca
     3721 ccccactgcc agaacagggt ttccaggtca tgctctgcca cccggtcact ccagcgtgca
     3781 taccagcttc cgactgtttc cggcgaatgg aatgccagtt cgatttcagc ctgagcgacc
     3841 agcgcctggt acgcacgggc tttctgctct gcctccctcg cctgctggcg gctgtacttc
     3901 tgcatgacca gcgcggattt atggcgactg cgttccctga cacgaaaccg caccgacggg
     3961 aacaagcgtt ctgctgcatc gcctggaaga ggaaggaggc agacctcgcc ctgtgtttca
     4021 atcagcacat cgatggcata cagccactga agacggctgc cgtgcagttc ttctgcggtc
     4081 agggacggca tgaaaaatcg caggtcagac agcgatatcc tgcggcttcc gttcagacag
     4141 cggaaaaacg cccccgcata cggatattcc gccagcgtgt ttccctggtg gcggcgtttt
     4201 tctgtctccg ccaccgcccg ccatgcggcc tgttgctctg cgctgagggg aaccagtgcc
     4261 tgtgaacgtg cgctcatgcc tgacctcctt ccgccatcag gatatggatg atgtcactga
     4321 cgctgtaccc ctgcgcgtcc agtgacgccg ccagtgccag tgaatggctg acggcttccg
     4381 gtgcaaaccg ccgctgtacc tgaaccaccg caaacggctc accaccggca ttcaccagaa
     4441 ccagcaccca gacctgagag acatcgcccg gtgaacataa cgccaggtga aaacgttcgt
     4501 gggcaggcgt gaaacggacc tggacgggga aaaatccgcc aaactcgctg ccgtactcgc
     4561 cgttcatcgt ccagttgtcc ggcgcatcca gttcccgcat cacggcgtgt gtcagctcac
     4621 gacgttcatc acttccggca gcccggatat cttcatattc ctgtgcgctc atggtctgta
     4681 atacattcag ggtcagttca gttttcatca cggtatctcc cttgctgccg ccccgtctcc
     4741 ggcggggcgg tgtggttgtt catcaggcgg catcagccag gttatcggtg cgttcagtgg
     4801 tatctgtctg tggatgtggt gcacacatcc agccaggcac ccagcggttg tctttcatat
     4861 ggtgctctgc atgttcagcc gcatcacctt tcttcatctt ctccgcgtcc cgtgcggcac
     4921 cggacagccc tgcctcattc agggctgcga taatctgcgg ctttttcagg tgtccgaaga
     4981 agtttgcttt tgtcggctgc caccagtcgc gcatgtgaaa gccgatggcg ctttccagcg
     5041 tgtcaagcgg actgcgtgac gtgtgaccac actcacgggt ctggacaccg ttaaggctgc
     5101 atgcggtgca gaaactgagc agggataaca gcacctcctg actgagtgac aggaatgtcg
     5161 tcatgtcccg tgaccatccc tccggtaaca gggcagcaag acgggctttt tctgccatca
     5221 gcgccatgaa tgcggcacct tccttccccg atggcgcatc gctggtcagc gaataatgtt
     5281 tacattccag gctgatttgt gctggtttac tgtacgctcc gctgccaaac acattcaggc
     5341 agagcgtcca tgccagcagt gtcagggatt tgtccggttg ctgcatcagt gctgcctgga
     5401 ctgccagcgt gcgttcggaa gacattttcg tcagcagtgg cagactgatt tcctccactg
     5461 acgctttctc ctgcacctgt tccgtacggt cagcgtcgtc agccacgtca tcctcactgc
     5521 gcaactgcac accacgctgg acacacacgt tgccataacg ccaggacacc accactccac
     5581 ctccggcacg catctccggc gtccacgccc tgaccttcgc catgcagcgc atcagcttca
     5641 tttctgcttc cagcaggtca gattcctcac actggttttc cagcgcgtcg taacgcgtca
     5701 tcagttcgtt cagacgttct tcttccgcct ccgtcagcac cgcttccggc tccggcagac
     5761 agcggtatgt tccggcatcc tcacggcact caccgacagg ctccatgcgt ccggcgcacc
     5821 attcccagcc ttcggcttcc cgaaggaact ctgcgacagc ctggagtttt tccagcaggg
     5881 cggcatcgag cgccacgcag tccacatagc caccttcgtc gtcgctgaac aaatcggtgc
     5941 gcagttcgtc tggcgagaag gcatcagccc ccacgaagcg gaatttactg ttccccgcca
     6001 ccgccacttc actttcggtc accagacgac gaatggtctg tacttccggt ttaccgcccc
     6061 atcccgactg acaggcggct tcaaacacct gcacctgacg cgcggtgtcg ttctccagcg
     6121 ccagcgcctg acagtgttcg gtggtgatgc ggtcttctgc cagcgcatcg aggatgacag
     6181 gcgcaaggtc agccagtttc agcattcgct gaacgtggcg gggtgaatag cccagcaaat
     6241 caccgatttg tgcaggtgtt ttgccttcct gcgccattgc gcggaatccg gcaatctgtt
     6301 cggcagggtg catatcccga cgctgaccgt tttcagtcat tgatgcagcc gtcgccagct
     6361 cctgcggaat aaccttcacg cgtacaggcc agtcagccgg aaggatgcca cgctctgcca
     6421 gcatgttgag tgctgccagt cggcgaccac ctgcggcgac accgtggcgg tcaccaggca
     6481 gggcatgaac aaccagattc tgcaacaggc caacgccctt aatggactcc gccagttcgc
     6541 tgacggactc tgcggaatac ggcaccgtgc gcacattcag cggtgattta atcagcgagg
     6601 ccagcgacac gctcacttcc tccgtctgct ccagcagggc agacaggact gtttcctgcg
     6661 tttttgcagg tttacggctg gatttacgct ccgtttttgt cttagactct gtaactgaca
     6721 tatatttctc ctttgaccgg gatggttgta tgtctgccgg acgcgttccc gtccggcacc
     6781 tctcttactt aagaatgccg aaattccgga tgacatggtt catccagtac ccggcaccgt
     6841 cctcaaaatt ccaggtacgc cagaccaggc cactgttatt gcggacaacc aggcgaaaat
     6901 gcgttccctg atcatcctcg ataaagacat tccggtactg tgcggcaacg gcttcagcct
     6961 gttcgcgggt aaaggagccg tcaggcagcc cctgaaatat tctgaaatat tcgctcatcc
     7021 tcatatctcc ggtgatgccc cgcactggac ggggcggttg tcactgtctt tcgttagaac
     7081 ggaatgtcgt catcgaagtc atacgcgggt tcagacggct gctgaggctc cggctgcgcg
     7141 gccttacgtc cacggccttt tgttttcgcg ctcccttttt tcgtcgtgtc agcactctgt
     7201 ggctgaccat tctgctgagg ctgcggttgc gcctgagcgt tctgctgtgg tgcactcccc
     7261 agcatctgca tggtgcccgt ggttttcaca aggatttcag tgacgtaacg ggtaatacct
     7321 ttatcttccc agctacgggt tcggagttgc ccctcgatgt agacctgcgc gcccttgcgc
     7381 agatattcac ctgccacttc cgcgagcttg ccgaacagca ccacgcgatg ccattctgtc
     7441 tgctcccgca tctcccccgt ctgtttgtca cgccagcttt ctgacgtggc cacctgcagg
     7501 tttgccactg cgcccccgtt ggggatgtaa cggacttccg gatcttttcc cagacgaccg
     7561 acaagaatga ccttgttaat gccacgaact gccatgatgt gtctccttct gttgatgttt
     7621 tcagccccca tcatctgttg agtttcggga gccgcggttt tgacgttttg cggggacctg
     7681 ctctttgttc cctccggtat tcaccatcac ctgtttgaga accttcgccg catggggcat
     7741 cactgccacg gcctgacgca cctgtcctgg gcagaacgca aaatttttta ccgggttttg
     7801 ggtaaaaaat tttgtgggct gttcatttac cctggacagg tgcggcaggt tgtggctgtc
     7861 tatgcccccg gcgacagggg cttaaacagg tgaatggcac cggacagaac aacgggcagg
     7921 gacccgccgc aacgacgtga agcgcggttt gcgaaacggc gttgctcctt acatcccggc
     7981 gtcttttgcc gggatgccgg gagcgcaggc cacggcgcga caagggtggc gcgaccgttc
     8041 cggtcgctgc aaacccgggt ttttgcgtca gggatggaag cccgccaggg cggagacagc
     8101 tccgctggct ccgttcacga cagcccgccc cgccaggggg acgcccggaa aaggcataag
     8161 caggagaaca acaaaacaga cagtaatcag agcagcagaa aaaagaagaa cagtagcagt
     8221 agcagtagca gtagcagtag caggaacaac aacatcatca gtaacaggca aatgacaggt
     8281 ctgaaaagac agaagcccac agcagaacgt ccgcgtaatg aaaggggcgg cgcgtaagcg
     8341 cggccccttt cagcgccacc ggcgcgcggt cttttcccgg cgcgggcaga cgggcattta
     8401 tcctcccgcc tgcccggatt catcaggcac gcagacgaat aatcatcgtc ggaacacggg
     8461 tataagcaaa tgtgccgcac ggcagcacct cgcggacatc agaaaacggt aacagcttct
     8521 cctgctggcg cggcccgttc agacagacgg caaccagaac gccccccgga cgcaacaggg
     8581 aaaaagcccg caggatatgc cggatatcct gcccgtgact gaacggcgga ttcataatga
     8641 tccggctgta atactgcacc ggctgccatt ccatgaagtc gccacactgt accctgacac
     8701 cgttaaagtt ttcccgcaga taccggacca gcccgctgtt aatctccacc gcatcacaca
     8761 tggcctccgg cgcggtatcc cgaatcgccc gcagtatggc cccggtgccg gcagagggtt
     8821 ccagaatgtg atcacggttg ctgatttccg ccagcgtcac caggcgatca cagaccgggg
     8881 caggagtcac aaaaagctga gattcagccc ggatttcagc aaccggcgcg gcgttatcca
     8941 gccttgcgcg catcagccgg atacgttctc ttaactgact gtgcatgtca ttatctccgt
     9001 catcccccgg ggatttcccg gggggatata ccttatttct gcgggatttc cgtgattttc
     9061 atgtcggtga tatacacatt caccagacgg aaattattgt ccattgtgcg gcggtagcgg
     9121 tatgccccgt ggtcttctgc ttcttccaca ctgcgcacgg ccttacagtc ccggggcagg
     9181 gcggcccact gcgcctttgt catttcccgg aaaccttccc ccggatagtt gactaccggc
     9241 ggacgcttcg cggcctgact ggcgacggca gcctcttctg ccgatggtgc tttgtagtcc
     9301 gtgatgcgat cgggcgtcac tttcatcgtg ccgctgtacc cgagaaaact gtaatttggc
     9361 gttgtgactg aactcaccgc cccgttgctt ttgttcacgc ggatgatagt cagccactcg
     9421 ccccggctga aaacctgtcc gcccggctca aaatcctgtg tccgggtaac cacgccgccg
     9481 ctttcgtcca gcatggcacg ctcatagatc aggcggttct gatagtggtt aacccagcgt
     9541 tgctgatgct gaatctgccg ttcatgacag cggatcgcaa tttcgcgggc ctgctccgtg
     9601 gtgatgatgt catcatccag ggccgaccat aacgaccggc tcccttcata ctggcttttt
     9661 tctgccgggc gcggatacgt atccagcggg aaacaggcgc tgatatggtc atgactgctg
     9721 atgagttttg ccatattcag atccagcgat tcagcccgcc acatcgtcag atatttctgc
     9781 gactgtgcga tggtcttttc agccttgcgc aaatccgctt cgattttttt gatacgacgc
     9841 cagcgaacgt ccgggcgttc tttatacttc gcgtgaagca gtgccgaccg cgcccgctcc
     9901 tcccagtatt ccgcacgttc aaagagcatc acggcacgtt tcatgccgtt ttcaatacgc
     9961 tgcgcatcac gacgggcgcg gcgttcgctg tgatgcccca caagaatggg ctgaccgggc
    10021 gggatcatcg cggccagtct ttccacttca tcaagtgcct gtgcggattc actggcccgc
    10081 tttccgctgt atccggtaaa ccgctccgcc cgtgcttcct gacgttcagc gagcgtgctg
    10141 tcttcatcct caatctctcc ggcaagtgag aggagcacgt cttcccggcc cggtgtccag
    10201 gccggcgcga caaacagcgc ctgtttgggt gcccagcgaa aaccggcatc atgcactttt
    10261 ttatacgtct ccgggtcaag acgtgacgcg gcatacaggc gcagtttgtt gtcatccggt
    10321 gagtaggtcg cgcggtacat ctgcacaggg ctggcggctt ccggggcggt catggtgatt
    10381 gctgctaatg tcataaaatg ttctccttcc gtctgttgcc gcccctcgcg gggcggctca
    10441 cctcatcagt taaaagcgcg tttcagttcg agtgtgcgga gcacctgccc gaaagccgtg
    10501 tttttcatgg cggggatgtg tacaccacgc ccccggtgaa aactccgggc tatgtgtcgc
    10561 ccgaaggctt tcaacacggg gtcaatttcc ggcgtgggga tgtcttcgcg tctgaaacgg
    10621 ggatgacggc ggggctttac agccgctgcg gaaggtgaaa caatgcttac agtcataaat
    10681 ggattcctta tgacaaattg gttaacaatg ccgtcaaaat tggtcgcaca attcttacgg
    10741 cgtggattaa gcaggtgcct gacctgcttt tttcatatct gcatctgacg ctttccggtg
    10801 ttttcctcct tctgttcctg cgttctcatt acctgtcagc cggatgactg acagaacaaa
    10861 taaatgcgta ttgccggagc ctgaaataaa tcctgatttt taaagaacat cacccgttga
    10921 gtttcgggag ccgcggtttt gacgtttcgc gggaacctgc tgctgtattc actgtggtat
    10981 gaacttcacc cgtttgagaa ccttcgccgc aaggggcatc actgccacgg cctgacgcac
    11041 ctgtcctggg cagaacgcaa aattttttac cgggttttgg gtaaaaaatt ttgtgggctg
    11101 ttcatttacc ctggacagtc agtgcggcag gttgtggctg tatcagcccc cggcgacagg
    11161 gactcatacg ggtgtgtggc acccggcagg caatgagcag gggcccgccg caacgacgga
    11221 gagcgcgtgc gcgaaacggc gttgcccctt acatcccggc ggccttttgc cgggatgccg
    11281 ggagcgcagg ccacggcgcg acagaggcgg cgcgaccgga acggtcgctg caagccaggg
    11341 gttttcacgc atcggaccgt agcggcagcc cgaaaccgcg ccagcgggtt cggcggagac
    11401 tggcaggtgc gccagcagct gcctgtcgga gtcgggcctg acgaccggat gccgccaggc
    11461 agccgggagc cgaaggtgcg cccggcagac agaacaacac tgcagtagtc agaatcagag
    11521 cagtagataa aggaacaaca acatcaggaa cagttccata acataagaaa gaaacactcc
    11581 tgagtcagac acatcacaga cgggaagggt aacgggcgac gcgccagcgt ggcccgcttc
    11641 ccttccggca ggcgtcagcc tgccaggggt tttcgcgcgg gctgacaggc ggcctccacc
    11701 gcctgcccct gtatcacctg tttatgctgc cctgaaggat gtagtgaccg ccgaggaata
    11761 cagcctgctg attcgccaca aaatcgcaga tttcatcaac aatctgcgca gtggtgagcg
    11821 gtttctgccc catttcacga cgccgcgcat taatcacggc tttgtaattg cgcagaattt
    11881 cgcgggtttc tgtgctgata ttcatgacat gtgctccccg tccagcgcct gtaacacaac
    11941 gtccattgct tcgcgttctt ccctgacgaa tgcgtcaccg tcgcccccgt tctcgctccc
    12001 gacggcatac aggcgcttca tttcactgcc tgccagcgcc atcacttttt ccagcatcac
    12061 cgccggaact gtcacctgac ggctggcgcg cttttcttcc atcagctcac gaacaacgtc
    12121 gtgaacaatg ctgacatccg caccatgcgc aaaggcatcg tccagtcgct gcatgacggt
    12181 ttccagctca tcgtctgtca gactccagtc ctgggctatg tgacgcacat ccccacgggt
    12241 cagcaccacg gcaaaaacgc attcactgtc cggcacgtct gtgttcagca cttcgcggat
    12301 aatttcttta acagtacagt acatgttctc gtctccttta caggggcggc attgccgccc
    12361 cgtccgttaa tcagtccagt gccgcgtaaa tcgcgttgca ttcggggtga aattcgatgt
    12421 gacgccacaa ctgcgcatcg cgcatccggt aaagctgggt cagtcctgca tccccgctgt
    12481 cgtggtacag ccacaactgg cggttaatca caagggaagt cagaatgatg cctgcggcat
    12541 cagcactcac ggtacggtca aaccagtttg caccgttcac catgtggaaa cggtcgccat
    12601 ccggcatcat gtaaccgcca ccgtccggca ggcggatgaa gtgccagaag cctccctgat
    12661 attccggcat cattttttgc gccagcgcgt acacatacgc ctcggcatac agaaaatcgt
    12721 tgccaaacag atacggtaga aaggagagac ggtcgcactc ttcaacgttc agagtgacag
    12781 gtttcgcata ttgcatgatg ttttcctccg ttgttcacat attccgcgac accgggcggt
    12841 gccgtgcctc cataaaagcc gggcgataag gcggggatgc acagggcgca agggaagccg
    12901 cagcctgacg cacacggctg gcgggagttg gagggtgaga ccgcgaaatc tgagcgggtg
    12961 aacgaccgga aaatcacggc agacgggaag gagggaccgg gtgaccgcag cggcgaagcg
    13021 tacccttgca tccctgccag cccggctaag atggaggcac ggcacagccc gcacacgacg
    13081 ctgaggccac ataactgaaa aaccacaagg ccatgccctg catggccggg taaagcggag
    13141 ccggcggcga cttcctttgt gctctgtccg gggaccggca ggaaaaaggc ggggatttac
    13201 cccgccaacg tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg
    13261 cccgggcttc ccggaccacc tcccgcagaa caccacggtc aaaccagcgg cggggcggtt
    13321 catccggtat acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat
    13381 cgctgaccag gtacatttcc accgacgaaa aacgtgctcc ccggttcacg atggcatcga
    13441 gcaccacggg caggttttcg tcatcttcat cgagccagca ggggtgatcg ctttccttca
    13501 caaccagacg aaagcggcgc gtctggcgag tcagcaacgt ctgaccggtg gtggtcagcg
    13561 ccgcccggat acgccgcgca tcttctgcca cccggcaact gacactgcgt accgcatcgc
    13621 gcaccgccgc aagtacagcc ctgatttcgt cttcggtcag ccattctccg gcatacggga
    13681 aagacacctg cgttccgtct gccggaagta ccggcagcga ctgccactga ctgccggaca
    13741 gtaccagttc cgccccggtt ccggtcagtt tctgaatggc ggaggccagg agcggcggca
    13801 gtacgccgcc gtgacggttg ataagtaacg tcgccagccg gggcgcatcc agcagaacat
    13861 aaatgctgat ccagcccgga tacttcgggt tacgctggtc aacatccgtc tcttccggcc
    13921 agccctgcgc cagcagcagg ttgcgggcgt gacgacaaat cagtgcgttc agcgtgctgt
    13981 tttcgttcat gattaagcgt tctccctgta tcgccgcccg cagggcaggc ggcgataaca
    14041 tcagtcgttt tcgtgtgtgt tgtttttcag cagggtgcgg gcctgattaa ccacctcctg
    14101 acggcgggtg acgcattccg ggacggcgag gccatgatcc cgggcggccc attcacgttt
    14161 ccacaatgcc tgttcagcgg tctgaatcag ggacgccagc agttcagcag ggacggtcac
    14221 caggcggttt tcgctctcgt tgcggataat ctccatcacc gcagcggaag aaataccgga
    14281 ctcaatccgc tggtcttccg ggatgtcctc caggcgtgcc aggacggtcc tgatttcatg
    14341 accggtcaga gaaatttcca taccgtcagc cagtgcctga atctcttccg gggaccagat
    14401 aaccagcatc agcggcatgt ctcccggata ctttactgcc agctcccggc atagcgtttc
    14461 gcatgttccg tacattctcg tctccttctc aatctttaaa gcacgcttcc agttccagaa
    14521 tttcggtata tgcctccatc aggaggcggt ttgcgcgaaa cagcgtctga cgcaccagac
    14581 gttccggcag ggtgtcggca ttaaagctgt accgctgctc catctcattc atggttgtac
    14641 gcaggtcgca catggcattg gccacctccg ccacttcggc gcgcagtttt tcgtgtcctg
    14701 catagttcat ttacgcagcc tccggcataa accagtcatc attcgcggcc ccctgctgca
    14761 tggcccgttg cacggcggca aggcgttgct gcccggcacg gtgatactgc tcaagcagct
    14821 cgataccgat ataccggcgt ccggactgga gggcggcgac gcaggttgag ccgctgcctg
    14881 caaacgggtc cagcacaatt gcgttcgggt gtgtgaagct ctcaatcagc ggttgcaggc
    14941 tggtaacggg cttttccgtc gggtgatggc gattgcccga atatttccag cccagcacgt
    15001 ccggcagcgg gttttgtggc agacgtggac ggccttttgc caggatgtag gcacattcgt
    15061 ggcgatagcc cacatatgcg gccttcgatg tgtagttttt ggtgaagacc aggtgaccaa
    15121 caacgctgaa tcccgcattt ttccaggcgg ccataaagcg atcgacgcgg ttccagccat
    15181 agaagctcac cattaacgcg tcttttttga gtacgcggta catttcatta caggctggtt
    15241 gcagccattc atcggtttta tcgccggcga tggtgcgccc ggaacggtca cggaaaccga
    15301 cgagatacgg cgggtcggtg aggatgaaat cgacagcatt gcccggaaac gtggccataa
    15361 cacgcacgca gtcacccagg ataaaacggg acataacagc actccttctc ttacagaggg
    15421 caccgggagg gcacccctgt ggtgcccgtt ccccgctgcc tgtgggctgt tcaccgcgtc
    15481 cggtaacggg tcaggccgac cgtggaatac cgcagccgtc gtctttttga cggcgaggat
    15541 atgccgcgaa agcggcttga agggcgttca tcagaaaagg gcgacgggct ggcgtacact
    15601 gtcaccaacg ccagccggga gcggtaaaag cgcccgtccc gtgcccggcc ggacgcggga
    15661 cactgaccac aggcagcgtt cctgccgcgc cgcgtcagcc ggcgcagtaa aaagaggagc
    15721 ccggcgacat cttttgttac ccgccaaaca aaacccaaaa acaacccata cccaacccaa
    15781 taaaacacca aaacaagaca aataatcatt gattgatggt tgaaatgggg taaacttgac
    15841 aaacaaaccc acttaaaacc caaaacatac ccaaacacac accaaaaaaa caccataagg
    15901 agttttataa atgttggtat tcattgatga cggttcaaca aacatcaaac tacagtggca
    15961 ggaaagcgac ggaacaatta aacagcacat tagcccgaac agcttcaaac gcgagtgggc
    16021 agtctctttt ggtgataaaa aggtctttaa ctacacactg aacggcgaac agtattcatt
    16081 tgatccaatc agcccggatg ctgtagtcac aaccaatatc gcatggcaat acagcgacgt
    16141 taatgtcgtt gcagtgcatc acgccttact gaccagtggt ctgccggtaa gcgaagtgga
    16201 tattgtttgc acacttcctc tgacagagta ttacgacaga aataaccaac ccaatacgga
    16261 aaatattgag cgtaagaaag caaacttccg gaaaaaaatt acattaaatg gcggggatac
    16321 attcacaata aaagatgtaa aagtcatgcc tgaatctata ccggcaggtt atgaagttct
    16381 acaagaactg gatgagttag attctttatt aattatagat ctcgggggca ccacattaga
    16441 tatttctcag gtaatgggga aattatcggg gatcagtaaa atatacggag actcatctct
    16501 tggtgtctct ctggttacat ctgcagtaaa agatgccctt tctcttgcga gaacaaaagg
    16561 aagtagctat cttgctgacg atataatcat tcacagaaaa gataataact atctgaagca
    16621 acgaattaat gatgagaaca aaatatcaat agtcaccgaa gcaatgaatg aagcacttcg
    16681 taaacttgag caacgtgtat taaatacgct caatgaattt tctggttata ctcatgttat
    16741 ggttataggc ggtggcgcag aattaatatg cgatgcagta aaaaaacaca cacagattcg
    16801 tgatgaacgt tttttcaaaa ccaataactc tcaatatgat ttagttaacg gtatgtatct
    16861 cataggtaat taatgatgga caagcgcaga accattgcct tcaaactaaa tccagatgta
    16921 aatcaaacag ataaaattgt ttgtgataca ctggacagta tcccgcaagg ggaacgaagc
    16981 cgccttaacc gggccgcact gacggcaggt ctggccttat acagacaaga tccccggacc
    17041 cctttccttt tatgtgagct gctgacgaaa gaaaccacat tttcagatat cgtgaatata
    17101 ttgagatcgc tatttccaaa agagatggcc gattttaatt cttcaatagt cactcaatcc
    17161 tcttcacaac aagagcaaaa aagtgatgaa gagaccaaaa aaaatgcgat gaagctaata
    17221 aattaattca attattattg agttcccttt atccactatc aggctggata aagggaactc
    17281 aatcaagtta ttttcttacc agtcattaca taatcgttat tatgaaataa tcgtttgcac
    17341 tgtctctgtt attcaggcaa tttcaataaa ggcacttgct cacgctctgt cattttctga
    17401 aactcttcat gctgcatttc gatttttctt ctaaaatcat aacttgcata cctgttcttt
    17461 ttatgtatat ccagatctat ttttgtgacg agttcaaacc acaatgatag cactccccat
    17521 gcagcgttat gaaacatttc cgcaacagat ttagagtctg tattaaccgc ttcagtcatg
    17581 tagtcgctaa tacgttctgc agtgattttc gttatttcat cgtaattcat tttatcccct
    17641 acaagactat ttaatggaat gaaaaatatt tggtctgata acaatgctcc tgaattaaca
    17701 cctgcaggct atgttaaagt tgccctcaca cacaatctac ctgttttctc gtgtgtgtca
    17761 caggcgcaat acataaatag tctggccatt aagttcgtaa cggatttcgt actcgccagc
    17821 aaaaatccgt ctgacatccc tggtttcaaa ctgaaacaac tgttcatccc atacgtggat
    17881 gagttaacag aatgaccggc gcctgtgtca gggactacac cgttttggct gcagctggtt
    17941 tactgaccaa cgccagaaaa tcatataact gcgtcaatgt cagcgtatcc agcgcgactg
    18001 tattattttt
//
LOCUS       DAZXUK010000058        17903 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZXUK010000058 DAZXUK010000000
VERSION     DAZXUK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17903
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.058"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACH3L8_001605"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACH3L8_001605"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFT4084768.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="ACH3L8_001606"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="ACH3L8_001606"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFT4084769.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACH3L8_001607"
     CDS             complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACH3L8_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFT4084770.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="ACH3L8_001608"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="ACH3L8_001608"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFT4084771.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="ACH3L8_001609"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="ACH3L8_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACH3L8_001610"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACH3L8_001610"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFT4084772.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACH3L8_001611"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACH3L8_001611"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFT4084773.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACH3L8_001612"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACH3L8_001612"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFT4084774.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACH3L8_001613"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACH3L8_001613"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFT4084775.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACH3L8_001614"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACH3L8_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFT4084776.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="ACH3L8_001615"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="ACH3L8_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFT4084777.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="ACH3L8_001616"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="ACH3L8_001616"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFT4084778.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACH3L8_001617"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACH3L8_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFT4084779.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACH3L8_001618"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACH3L8_001618"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFT4084780.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACH3L8_001619"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACH3L8_001619"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFT4084781.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACH3L8_001620"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACH3L8_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFT4084782.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
BASE COUNT         4183 a         4551 c         4811 g         4358 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gca
//
LOCUS       DAZXUK010000059        17867 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZXUK010000059 DAZXUK010000000
VERSION     DAZXUK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17867
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.059"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            50..689
                     /locus_tag="ACH3L8_001621"
                     /pseudo
     CDS             50..689
                     /locus_tag="ACH3L8_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            792..1115
                     /locus_tag="ACH3L8_001622"
     CDS             792..1115
                     /locus_tag="ACH3L8_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HFT4084783.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1182..2639)
                     /gene="nuoN"
                     /locus_tag="ACH3L8_001623"
     CDS             complement(1182..2639)
                     /gene="nuoN"
                     /locus_tag="ACH3L8_001623"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFT4084784.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2646..4175)
                     /gene="nuoM"
                     /locus_tag="ACH3L8_001624"
     CDS             complement(2646..4175)
                     /gene="nuoM"
                     /locus_tag="ACH3L8_001624"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFT4084785.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4339..6180)
                     /gene="nuoL"
                     /locus_tag="ACH3L8_001625"
     CDS             complement(4339..6180)
                     /gene="nuoL"
                     /locus_tag="ACH3L8_001625"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFT4084786.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6177..6479)
                     /gene="nuoK"
                     /locus_tag="ACH3L8_001626"
     CDS             complement(6177..6479)
                     /gene="nuoK"
                     /locus_tag="ACH3L8_001626"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFT4084787.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6476..7030)
                     /gene="nuoJ"
                     /locus_tag="ACH3L8_001627"
     CDS             complement(6476..7030)
                     /gene="nuoJ"
                     /locus_tag="ACH3L8_001627"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFT4084788.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7042..7584)
                     /gene="nuoI"
                     /locus_tag="ACH3L8_001628"
     CDS             complement(7042..7584)
                     /gene="nuoI"
                     /locus_tag="ACH3L8_001628"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFT4084789.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7599..8576)
                     /gene="nuoH"
                     /locus_tag="ACH3L8_001629"
     CDS             complement(7599..8576)
                     /gene="nuoH"
                     /locus_tag="ACH3L8_001629"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFT4084790.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8573..11299)
                     /gene="nuoG"
                     /locus_tag="ACH3L8_001630"
     CDS             complement(8573..11299)
                     /gene="nuoG"
                     /locus_tag="ACH3L8_001630"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFT4084791.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11352..12689)
                     /gene="nuoF"
                     /locus_tag="ACH3L8_001631"
     CDS             complement(11352..12689)
                     /gene="nuoF"
                     /locus_tag="ACH3L8_001631"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFT4084792.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12686..13186)
                     /gene="nuoE"
                     /locus_tag="ACH3L8_001632"
     CDS             complement(12686..13186)
                     /gene="nuoE"
                     /locus_tag="ACH3L8_001632"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFT4084793.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13189..14991)
                     /gene="nuoC"
                     /locus_tag="ACH3L8_001633"
     CDS             complement(13189..14991)
                     /gene="nuoC"
                     /locus_tag="ACH3L8_001633"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFT4084794.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15085..15747)
                     /gene="nuoB"
                     /locus_tag="ACH3L8_001634"
     CDS             complement(15085..15747)
                     /gene="nuoB"
                     /locus_tag="ACH3L8_001634"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFT4084795.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15763..16206)
                     /gene="nuoA"
                     /locus_tag="ACH3L8_001635"
     CDS             complement(15763..16206)
                     /gene="nuoA"
                     /locus_tag="ACH3L8_001635"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFT4084796.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16837..17775)
                     /gene="lrhA"
                     /locus_tag="ACH3L8_001636"
     CDS             complement(16837..17775)
                     /gene="lrhA"
                     /locus_tag="ACH3L8_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFT4084797.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4453 a         4988 c         4606 g         3820 t
ORIGIN      
        1 attcattata aataataatg aaataacgcc ttttattgat aatttacata tgaaaaaatt
       61 ttccttttgt tatattattt ctgttttcac tccctctgat tatttttacc aaccctgggt
      121 cactgcatta tcgccaacgc cccgacacgt aagtcctgag caattagaaa aaacagttcg
      181 ttatcttaca caaactgtgc atccacgtaa tgccggcaat attgataatc tgaataggtc
      241 cgcagagtat ataaaagagt tctttgtcag tagcggtgcc agagttaccg cgcagggcgt
      301 ccccgattgg ctattacgac tctgcgcctg gaagccagaa ttacccttac ccggcaatgt
      361 cctggcttta tcccgatcgg ggagacttta ttgctgtggt cggcagaatg caggatatca
      421 acgccgttcg tcaggtaaaa gcggcattgt tgtcatcccg ggatttatct gtttattcta
      481 tgaatgcccc agggtttatt cccggtattg atttttctga tcacctcaat tattggcaac
      541 acgatattcc cgccgtaatg attactgaca ccgcttttta tcgtaataaa caataccact
      601 tgcccggtga taccgcagac agattgaatt atcagaaaat ggctcaggtg gtagatggtg
      661 ttataacctt gttatacaac agtaaataat aaaaatttct cgaggcgggt ccagaaacag
      721 cgacatgtac attttatttt ccctcgtctg ttttaagatg cgaaacataa aattcaatgg
      781 aggaaaaata aatgggaatg attggctatt ttgcagaaat tgactcagaa aagattaacc
      841 aactgcttga gtctactgaa aaacctttga tggataatat acatgacacg ctttcaggat
      901 tacgccgact ggaatcactg gatcgaagtg agctgcgtaa acagttttcc attaagcgac
      961 tcaatgaaat ggagatttat ccgggagtga ccttttcaga agagttagag gggcaactct
     1021 ttgcctcgat catgcttgat atggaaaagc tgattagcgc gtatcgacgt atgctgcgac
     1081 aagggaatca tgcgctgacg gttattgttg ggtgatggtg tcagagcgcc aaaaaaaacc
     1141 gccgaacatg tcggcggctt tctgacttac aaactaacag tttacatcag cggcattgct
     1201 aaccgcacaa tactaatcag cggttgtggc catacaccca gcaccagtac caacagtgca
     1261 gaaatcagca ccacgatacc gcccgcgctg tactgccagt ttgacggtgc atcgcgaccc
     1321 ggttgttccg gtgcgtgcag gtacaggctc accgccacgc gcaggtagta gtagaggccg
     1381 attgccgaac cgacaaccac ggcacccacc agccaccaca agtgtgcctg gacaccgacc
     1441 gccagcacgt agaacttacc gataaagccc agcgtcatcg ggataccggc cagagacagc
     1501 atcatcaccg tcatcactgc cgcgaggatc ggacgatgcc agaacagacc gcggtaagag
     1561 aacagggaat cagcatccgg gccacgatac gggctggaca tcaggctgac cacgccgaac
     1621 gcgccgaggc tgctgaacag ataaccggcc aggtaaaccc ctaccgcttc catcgacatc
     1681 tcgccggttt gcagcgcaat cagcgccacc agcagatagc cgaggtgaga gatagatgag
     1741 taaccgagca gacgtttgat gttggtctgg ctcaacgcca tcaggttacc gaagatgatg
     1801 gaggcaaagg cgataatcgc cagtactacg cgaatcgctt cgctgtcacc caccggtgcg
     1861 tagaggaaca gacgcatcac cacaccgaag atagcgattt tgctcgccgt cgccaggaaa
     1921 gtggaaaccg gcgcaggcgc gccctggtat acgtctggcg tccacaggtg gaacggcacc
     1981 agagagagtt tgaagccgag gccaacaatc atcatgccga aacctgccag caacagcggc
     2041 tcgttgagca taccgtcgcc gaggttttta cccagcgcga caaacgacag gtcgccagac
     2101 tgcgcataca ccagcgccat accgaacagc aggaaagaag acgctgcggc agaaaggatg
     2161 gtgtatttga tactggcttc cagcgaacgt ttctggcgga aagcgtaacc gaccaggcca
     2221 aacagcggca aagagatcag ttcaataccg aggaacagag acgccagatg gtttgcattc
     2281 gccagcagga tcccgcccag cgcggcaatt aacaccaaca ggtagaactc atccttgttg
     2341 tcgttatagc cttcaagcca cgggtaggcg aaagtacagg tggcgaggct cgccaacaat
     2401 accagcccgg tgtaaagcat ggcgaaacca tcaacgcgca tcagcggcgt aacgtccata
     2461 gcgcccgcct ggccaacaaa ccagagcgaa accagcgccg cgttaagccc aataaccgag
     2521 agcgtagcgt tgaggaaatg attgcgtcgc cacgcaatgg agagcatcac aaccaccacc
     2581 gtcaagccga cgatcagcaa cggtagcagt gcgatcaggt tttgtggagt tattgtcatg
     2641 gcgatttacg gccttgtagt agtaacggaa ttaacaaacc actgctggat attgccaatc
     2701 gcggagtgcg aggtatccag aatcggctgc ggatagaagc ccagcagtac cagcagcacc
     2761 accagcaaca ggatcataaa cagctcacgc agcgacatcc ctggcagttc ctggctggca
     2821 atctggcttt tcgctttacc gaagtaagcg cgatgcaaca tcgccagcga ataaacagat
     2881 gcaaagacca gcccaaaggt agagataacg gtaatcaccg ggacaacctg gaagctgccg
     2941 aacagaatca taaattcgcc gacgaagtta ccggtgccag gcatcccaag cgttgccacc
     3001 gcaaagaaca gcgacagtgc tggcagccat ttcatcttgc tccacagacc gcccatcatg
     3061 cgcatgtcgc gggtatggat acgttcataa agctgaccgc agagaataaa cagacccgcc
     3121 gccgacaagc cgtgcgcaat catctggatt accgcgccct ggtaggccaa ctggctgccg
     3181 gtgtagattg caatcagcac gaagcccatg tgggaaaccg aggtgtaggc gatcagacgt
     3241 ttgatatcgg tctgagcgaa ggtcatccac gcaccgtaga agatgccgat aacacccagc
     3301 cacatagcga ttggcgcgaa ctctgccgac gcgttcggga acagcggcag ggagaaacgc
     3361 aacaaaccgt aagcggcggt tttcagcaag atccccgcga ggtccacgga accggcggtc
     3421 ggagcctggg agtgcgcatc cggcagccag ccatgcagcg gaaccaccgg cattttgact
     3481 gcgaaggcga tgaagaagcc cagcatcaac agatattcca caccactgga cattggcgta
     3541 ttcagcagct cttcatagtt gaaggtccag acgccggtcg cattgtagtg aacaaaaacc
     3601 agcgccagga tggcgatcaa catcaccaga ccactcgcct gggtgtaaat gaagaacttg
     3661 gttgccgccg tgatacgcgt tttaccgtca gaggctttat gcccccacag tgcgatcagg
     3721 aagtacatcg gcaccagcat catttcccag aagaagaaga acaggaacat gtcgatggca
     3781 aggaacacgc cgataacgcc gcccaggatc cacatcaggt tgaggtggaa gaagccctga
     3841 tatttttcga tctctttcca cgaacatagt accgccagca caccgagcag accggtcagc
     3901 acgaccatca gcagcgacag accgtcaatg gcgagatgaa tagagatacc aaaacgcggg
     3961 atccacggca tgtcgaattc agactgccac tgcggaattc cggcggattg cgtcagtgaa
     4021 taaccgccct gcaaccacag ttgcagcgac agcgccagcg tcaatcccat ggtgatcagc
     4081 gcgatccagc gtggcacctt gacgccaaag cgttcggtct gccagcacag gaagccgcca
     4141 ataaagggaa ttaatattag ccagggtagt aacatggcga tctttattcc ttgtaaaagt
     4201 cccgtcagga ccggattttc aacgaattct cacgacagaa aattctcttc ggactggggg
     4261 tagtgtcgga tgcgccgcga aggcagctta tccgacctac gttcttatat gcccaggggc
     4321 aatcccacaa tccttaactc aacgcagtac catcaacagt gccagcacca cgaccgcacc
     4381 gatgctcatg gatgccacat accagcgcag ataaccgttc tcgcttaaca gcagaccttt
     4441 acctgcaaag cgggaaagaa ccgccgggat gttcatcatt gagttcagcg gatcgcgttt
     4501 cagcaaccag gcaataccca ggaacggctt gacgaacact ttgtcgtaca gccagtcaaa
     4561 gccccaagcg ttgtaccacc aggtactcag caggcggccc ggcgcactgt tggcgatgga
     4621 ggtcaccaga gaacgtttac ccagccacag ccaggctgcc agcagaatgc cgaccaccgc
     4681 gaccacgcca gaggtaattt ccagggtcaa catgctgccg tgcgccagtt ccgtcgtttg
     4741 cggaagcacg ccctgcagcg gcggtacaat cagtgcgcca acgaaggtgg aaaggatcag
     4801 cagcacaatc agcggcaggc tgtgagttac ccctttcacg gcgtgagcgt gaatttgttc
     4861 ttttccgtgg aagacgatga aaatcatacg gaaggtgtag agcgaggtca taaacgcacc
     4921 gaccagacct gccaccatca gattgatatg accattcgcc atcgcacccg cgaggatctc
     4981 atccttactg aagaagcccg cagtgaccag cggcagtgcc gacagtgctg cgccgcccac
     5041 caggaagcag agataaacca gcggaataga tttacgcaga ccgcccatct tgaagatgtt
     5101 ctgttcgtga tggcaggcca gaatgacgga accggatgcc aggaacagca gcgctttaaa
     5161 gaacgcgtga gtcatcaagt ggaaaatcgc cgcatcccat gcctgcacgc caagcgcgag
     5221 gaacatgtag ccaatctggc tcatggtaga gtaagcgaga acacgtttga tgtcggtctg
     5281 taccagcgcg gcaaaaccgg ccagcagcag cgtaaccgcc ccgacaatac ccaccagatg
     5341 cagaacttcc ggcgtcatca ggaacaggcc gtgggtacgg gcgatcaggt agacacccgc
     5401 ggttaccatc gttgcggcgt ggatcagcgc ggagacaggc gtcgggcccg ccatcgcgtc
     5461 agcaagccat gtctgcaacg gcaactgcgc agatttaccg accgcaccgc ccagcagcat
     5521 cagcgtcgcc cacatcagca tgttattgcc gtcagcaaag tgcgctggtg ccagttccac
     5581 catatcgcgg aagttcaggg tgcccagttc gttgtaaaga atgaacagtg cgaaagcgag
     5641 gaaaacgtca cccacacggg tcacgacgaa cgctttcatt gctgctgcgc cattcttcgg
     5701 atcggtgtaa tagaaaccga tcagcagata ggagcacagg cccacgcctt cccagccgag
     5761 gtacatcagc agcaggttat cggcgagaac cagaactacc atgctggcga tgaacaggtt
     5821 ggtgtaggcg aagaagcgag agtagccctc ttcaccgcgc atgtaccagg aggcgtacat
     5881 gtgaataagg aaacccacac cagtgaccac cgagagcatg gtcagcgaca ggccgtccag
     5941 caccaggtta aaaccgatgt taaagtcgcc taccgacatc cacgtccaca gcggctggct
     6001 gtatgcctgc tcgccgttag cgaagaaatc aacgccgata aaggcggtta ccagcgccgc
     6061 caggcccaca gagccaacgc cgacgatcgc cgaaacgttt tcagaccagc gcccacggga
     6121 gaacgccagc aggacgaagc caatcaatgg caaaataatg gttaaggcaa gcatgttcat
     6181 ccgcgcatct cacttactga atcgatgttc aggttctggc gacgacggtg aagttgcagc
     6241 agcagcgcaa ggccgatact cgcttctgcc gccgcgaggc tgatggcgag aatgtacatc
     6301 acctgaccgt cggtctgacc ccagtagctt ccggcgacca caaaggccag cgcggaggcg
     6361 ttaatcatga tttccagacc aatcagcata aacagcagat tgcgacggat aaccagaccg
     6421 gttaagccaa gaacgaataa gattgccgcg aggatcagtc catgttgtaa ggggatcatg
     6481 cgtgctcctc cgtttttctt ttcgcgctgt cgtctttacg attgctcagc acttcacccg
     6541 cacgctcttc acgaccgacg tggaaggcca caaccagacc cgcgagcagc agcatagaag
     6601 ccagttccac cgccagtacg taaggcccga acagcgtaat acccactgct ttagcactga
     6661 ttggcgtacc gtcgataccc tgatcgttaa caccgaggat ggcgtaaaca atcaccacca
     6721 gcatgatggc cgacaaaatt gccgggccaa tccacacctg cggtttcagc cactggcgtt
     6781 cctgttcgat ttctgaaccg cccaggttga gcatcatcac cacgaacacg aacagcacca
     6841 taatggcacc cgcgtagacg ataatttcca gcgcaccggc gaagtaagcg cctagtgaga
     6901 agaacacccc ggaaatcgcc agcagcgaaa taatcaggta cagcagtgcg tgtaccggat
     6961 tggtatgggt gatcactcgc aaggtcgcaa gtatggctat caggccacag atataaaaag
     7021 cgaactccat tgcccctctc cttacggtaa caggctcttg acgtcgatag gcttggcttc
     7081 gttctctgct tcgcccttat ctttgccgtc gattgccata cctgccatcc ggtagaagtt
     7141 atattccggg tatttgcccg gaccggagat cagcagatcc tctttctcgt aaaccagatc
     7201 ctggcgcttg tattccccca tttcgaaatc cggggttaac tgaatcgccg tggtcggaca
     7261 ggcttcttcg cacagaccac agaaaatgca gcgtgagaag ttaatgcgga aaaattccgg
     7321 ataccagcga ccgtctttgg tttctgcttt ttgcagcgag atacagccga ccgggcaggc
     7381 taccgcacag aggttacagg ctacgcaacg ctcttcgccg tccgggtcgc gggtcagaac
     7441 gatacgacca cgataacggg gcggcagata gaccggctct tccgggtaca ttcgcgtttc
     7501 gcgtttggcg aacgcgtgca ggccgatcat ccagatacta cgaacctggg tgccgaaacc
     7561 tactaacaat tcttttaagg tcatggtctt attgcccctt attgcgcctg ccagagaatg
     7621 acagccgccg ttaccagcaa gttgatcagc gtcagcggca ggcagatttt ccagccgaag
     7681 gacattaccc ggtcataacg cggacgcggt aacgacgcac gaatcaaaat gaacatcatc
     7741 ataaagaacg cggttttcag cgcgaaccag atgaatggcg gtaacaacgg gccttgccag
     7801 ccaccgaaga acagcgtcac catcaatgca gagatggtca caatcccgat gtactcaccc
     7861 acgaagaaca gaccgaactt cataccggaa tattcaatgt ggtaaccatc cgccagttcc
     7921 tgctcggctt ccggctggtc aaacgggtga cggtgacaca ccgccacgcc cgcgatggca
     7981 aaggtaataa aaccaaagaa ttgcgggatg acgttccaca catgcgcctg gctgttgacg
     8041 atgtcggtca tgttgaatga accggcctgc gccaccacgc ccatcaggga aagcccgagg
     8101 aacacttcgt agctcagggt ctgcgcagaa gcacgcatcg cacccagcag cgagtatttg
     8161 ttgttacttg accagcccgc aaacagcacc gcgtaaaccg ccagacctgc catcatcagg
     8221 aagaacaaaa tcccgatgtt caggtcggca accacccaac ccggactgac tggcacaatt
     8281 gcaaaggcca gcagcagcga ggtaaaggca atcatcggtg ccagggtaaa gatgacgcga
     8341 tccgagaatt tcgggatcca gtcttcttta aagaacattt tgatcatgtc cgcaaccagc
     8401 tggagcgaac cgccccagcc aacacggtta ggtccataac ggttctggaa cagacccagc
     8461 aggcgacgtt cgccaaagct catgaatgcc ccacaggtga caaccaccag caggatcacc
     8521 accgctttga ggatggtcag caggatctca atcagttccg gtgatatcca actcattgtt
     8581 gtgcctcctt gagatcctca agatgcgcgc cagccagcac cggagcaatg ccggacatac
     8641 ccatcggcaa gcccacctgc cctgccgtca gtccttcggc gatttcaacc ggcagcgtga
     8701 ccgtgttgcc atcgtaacta aaggagacgc gtgtacctgc gttcacaccc aacttcgcgg
     8761 catccgctgg gttgagtttg atgtacggct gcggcatacg gctctggaag accggagcac
     8821 gctgtgacaa ttcatcgctg ccaaacaggt ggtagtacgg cgcgatacgc catttcccgt
     8881 cctgcggctg gaagcgtgcc ggtacgctgg tgaagtaatc cagaccgttt tcgctggttt
     8941 caaacagacg cacgcccgga tcgccaaagc gcagtttgcc gcccacttcg tcctggaatt
     9001 tgttccacgc ctgcggggag ttccagcccg gcgcccaggc aaacggcact tgcgaacggt
     9061 gcgcggtcgg ctggttgtta ccttccatcg agaaggtgaa catggtgtca atatcctgcg
     9121 gctgacgcgg ctcatgaacg ctgatattgg cgcgcatggc ggtacgaccg ctgtaacggt
     9181 gcggttcacg ggccagtttc tgcccacgaa tacggaatgt cgcatccggc gcagcatctt
     9241 tgatacccgc cagttctggg attttcgcca caacagcgtc aattacatgg tcgagctgcg
     9301 tccagtccac ttcacggctc agcaaggtgc tgtgcagcga gtgcagccag cgccagcttt
     9361 ccagcatgac agttttgctg tcgtaataag ccggatcgta aacctggaag aaacgttggg
     9421 cgcgaccttc attattaatt accgtaccgt cgctttcagc aaagctggca gaggaaagca
     9481 ccagatgggc gttttccatg atcgctgtgc gttgatgatc aaccaccatc accagcggcg
     9541 ctttagccag cgcagcattc acgcgggtag cagaagcgta acgatgcaga tcgttttcca
     9601 acaccaccac cgcgtcggcg cgtccggttt ccagttcggt taacgcttct tcaagcgaac
     9661 cgccacccat aatgcccagc cccatgctgt tgacggaacg ggcaatcatg gtgataccaa
     9721 cgtcagcgcc gcgacctttc agggctttcg cgacgttagc cgcagcctga atcacctcta
     9781 tgctaccggc gttcgtcccg gagataatca acggtttctt cgcacctgcc agtgcctgca
     9841 cgatgacgtc gattttgttt tgcagctcag gttcgatacc gtcaaccgct ggtgcggagt
     9901 tatccagcgc atgggcgatg gcaaaaccta aacgcgcctg atcttcaacc ggtgcgcggt
     9961 aagtccacgc cgcgatatca tccagacgag tgtcatcaac gttggtaaca aacagcggat
    10021 gcttcgcacg ttgaccgatg ttgaggattg ccgcaatctg ccagtcagcc actttctgtg
    10081 ctgcagccat ttcgcgcgct ttacctttca ccgcctgacg cactgccagt gcgacgcgcg
    10141 cgccggtctg ggtaacgtct tcgcccagca ccagtaccgc atcgtaagat tcgatttcgc
    10201 gcagagccgg agtataaatg ccgccttcac gcagcacttt cagcgccagt tgcagacgtt
    10261 cctgctcacc gtgagcgata ccggtgtaga agttttcttc gcccaccagt tcacgcagcg
    10321 caaagttgct ttccacgctg gcacgcggag aaccaatacc gatcactttc ttcgactgac
    10381 gcagaatatc tgccgcgccc tgcattgcct gttcggcgtt gagggtaatg aaatcatcgc
    10441 cacgacgctg tactggctga cgcggacgat ccttcaggtt gacgtaaccg taaccgaaac
    10501 gaccacggtc gcagaggaag tagtggttta ccgtaccgtt gtaacggttt tcgatacgac
    10561 gcagttcgcc gtaacgttca ccggggctga tgttacagcc gatggaacat tgctggcaga
    10621 tgctcggcgc aaactgcata tcccatttac ggttgtaacg ctcggagtgc gttttgtcgg
    10681 taaatacgcc ggtcgggcaa atttcgacca ggttaccgga aaattcgctt tccagcgtgc
    10741 cgtcttccgg gcgaccgaag tagacgttgt cgtgcgcgcc ataaacgccc agatctgtac
    10801 cgtcagcgta atctttgtag taacgcacac agcggtagca ggcgatgcag cggttcattt
    10861 cgtgagagat gaatggcccc aaatcctgat tacggtgggt acgtttggtg aaacggtagc
    10921 gacggaagct gtgtccggtc atcacggtca tatcctgaag atggcagtta ccgccttctt
    10981 cacataccgg acagtcgtgc gggtggttgg tcattaacca ctcgaccaca ctttcacgga
    11041 actgtttcgc ttcttcgtcg tcaatggaaa taaaggtgcc atcggaagcc ggtgtcatac
    11101 aggacatcac caggcgacca cgcgtgtctt ccgcgttttg gtattgcttc accgcacact
    11161 ggcggcaagc accgacgctt cccagcgccg gatgccagca aaagtaagga atatcaaggc
    11221 ccagagacag acaagcttcc agcaggttgt ccgctccgtt gacctcgtat tctttgccgt
    11281 ctacatgaat tgtagccatt agcatgcttc cagttttctc ggtcagggac cgagcgttaa
    11341 tcgaaattcg gttaccagcg ctctttcagc aggttcggct gaatcccatt aatcaaatgg
    11401 gtattgctga acggctgttt gattcccgcc tcaaattctt cgcggaaata tttgatggcg
    11461 ctctgtaacg gctccactgc accaggtgcg tgggcacaga aagttttacc cgggcctaag
    11521 aatcgacaca gttgctcaag tgtttcgata tcgcccggct ggccttcgcc acgctccagc
    11581 gcacgcagaa ttttcacgct ccacggcaga ccgtcgcggc acggcgtaca ccagccgcag
    11641 gactcacggg cgaaaaactc ttccaggtta cgcaccagcg acaccatgtt gatctcatgg
    11701 tcaaccgcca tcgccagcgc cgtacccaga cggctgcccg ctttaccgat actttcgaat
    11761 tccatcggca gatcaaggtg cgcttcggtc aggaagtcag tccccgcgcc gcctggctgc
    11821 caggctttaa atttcagacc atcacgcata ccaccggcgt aatcttcgag gatctcgcgt
    11881 gcggtggtgc cgaacggcag ttcccacagt cccggatttt tcacccgacc ggagaagccc
    11941 atcagcttgg tgccagcatc tttacttttc gagatgttct gataccactc cacgccgtta
    12001 gcgaggatcg ccggaacgtt acacagggtt tcgacgttgt tgacacaggt cggtttgccc
    12061 catacgccgg aggttgccgg gaaggggggt ttcgagcgtg ggttagcacg acgcccttcc
    12121 agggagttga ttaacgctgt ttcttccccg cagatgtagc gccctgcccc ggtatggacg
    12181 aacagttcga agtcgaaacc tgttcccata atgtttttgc caagcaagcc cgcttcagtg
    12241 gcttcggcaa tggcgcggcg cagatttact gccgcttcga tatattcgcc acgcaggaag
    12301 atgtagccac ggtaagcttt cagcgcaaac gcggagatga gcataccttc caccagcagg
    12361 tgcggcagtt gctccatcaa caggcggtct ttataggtgc ccggctccat ttcatcggcg
    12421 ttacacagca ggtaacggat gttcatggat tcgtctttcg gcatcaggct ccacttcagg
    12481 ccagtcgaaa agcccgcgcc gccgcgccct ttcagaccag cgtcttttac ctgattaacg
    12541 atttcgtccg gagacagccc ggtcagcgcc ttacgcgccc cttcgtaacc gtttttgctg
    12601 cggtattcgt ccagccacac tggctgtttg tcatcgcgca gacgccaggt cagcggatgc
    12661 gtttcgggag tacggataat gtttttcatt tataccgctc cagcagttca gggatcgctt
    12721 ccggggtcag atgcgcgtga gtgtcctcat cgatcatcat gtttggccct ttatcacagt
    12781 tccccaggca gcaagttggc agcagcgtaa agcggccatc aaatgtcgtt tgccctggtt
    12841 tgatgttcag ctttttctcg agcgccgcct gaataccctg atacccgttg atatgacaga
    12901 ccacgctgtc gcaataacgg atcacatggc gaccaaccgg ctggcggaag atctgactgt
    12961 agaacgttgc cacaccttcg acgtcgcttg ccggaatacc cagcacatcg gcgatcgcgt
    13021 ggatcgcacc atccggcacc cagccacgct gcttctgaac gattttcagc gcttcaatgg
    13081 acgccgcacg cgggtcttcg tagtggtgca tctcgtgctc aatcgcttca cgctctgccg
    13141 cactcagctc aaaagcctcg gtttgtggtt gttgattctc gtgcataatt agcggtccac
    13201 atctgacata acaaaatcga tactgcccag ataaacaatc aggtcagaca ccaggctgcc
    13261 gcggatcgcc gccggaattt gctgcaaatg cgcataactc ggcgtgcgga tacgagtgcg
    13321 ataactcatg gtgctaccgt cgctggtcag gtagtaactg ttgatcccct tggtcgcctc
    13381 aatcatctgg aaagattcat tggcaggcat caccggcccc cacgacactt gcaggaagtg
    13441 ggtgatcagg gtttcgatat gttgcagcgt gcgctctttc ggcggcggcg tggtcagcgg
    13501 gtgatccgct ttgaacgggc cttccggcat gttgttgagg cactgctcaa gaatgcgcag
    13561 actctggcgc agctcttcca ctttcagcat tacgcgggtg tagcagtcag aaacaccacc
    13621 accaaccggg atttcaaagt cgaagttttc atagccagaa taaggacgcg ccttacgcac
    13681 gtcgaagtcg atcccggtag cacgcaggcc cgcgccagtg gtgccccact ccagcgcctc
    13741 tttcgcgcca taggcggcaa cgccctggga acgacctttc agaatggtat tttgcagcgc
    13801 cgctttctcg taagacgcca gacgtttcgg catccagtcg aggaactcac gcagcagacg
    13861 atcccagccg cgcggcaggt cgtgcgctac gccgccaata cggaaccacg ccgggtgcat
    13921 acggaaaccc gtgatcgctt ccaccagatc gtaaattttc tgacgatcgg taaaggcgaa
    13981 gaacacgggc gtcattgcgc cgacgtcctg aataaaggtc gagatgtaca gcaggtggct
    14041 gttgatacgg aacagttcgg agagcataac gcgaatgacg ttaacgcgat ccggcacggt
    14101 gatcccggcc agtttctcta ccgccagcac gtaaggcatt tcgttaacgc agccgccgag
    14161 gtattcaata cggtcggtat acggaatgta gctgtgccag gactggcgct cgcccatttt
    14221 ctccgcacca cggtggtggt agccgatgtc tggtacgcag tcgacaatct cttcgccgtc
    14281 gagttgtaaa acgatacgga aagccccgtg cgccgacggg tggttcggac cgaggttgag
    14341 gaacatgaag tcctcatttt cggtgccgcg cttcatcccc cactcttccg gtttgaaggt
    14401 cagggcttcc atctccagat cctgtttggc tttggtcagc tcaaacggcg agaattcggt
    14461 agcgcgtgcc gggtaatctt tacgcagcgg gtgacctttc caggtttgcg gcatcatgat
    14521 gcggcgcagg ttcgggtgac cgtcgaaagt aatgccaaac agatcccagg tttcacgctc
    14581 ataccagtta gcgttcggga acagtttggt gaaggtcggt acgtgcaggt cgttttctgc
    14641 cagcgccacc ttcagcatga tgtcgcggtt acgatcgata gaaatcagat ggtagaaaac
    14701 ggaaaaatcc gcggcaggta acccttcgcg gtgtgtgcgc agacgttcgt ccatgccgtg
    14761 taagtcaaac agcatgacgt aaggtttcgg cagtttcttt aagaaatcgc caacttccag
    14821 taattgttca cgcttgatcc acacaacggg aaccccggtg cgagtcgcct gaacagtaaa
    14881 ggcatccggc ccaaaacggt tgcgcagttc gccaattacc ggatcatcaa gatgatcgcg
    14941 ggtctgccag gcgggttctt gcgcggttaa gtcggtcata ttgttcacca ttgcaaatgg
    15001 tccgtggtga ctgtcggcgc agtaagcttc gcgaaataga tttaggaata agcgaagaaa
    15061 aatccctttg ccgacaggcg caaattaaat ctcgtcaggt gtacgcaggt tggttacggc
    15121 aatgcgttca ccgcgcttgc gttcgcgctc tgattgcata ttggcgcgat aaacgccctg
    15181 atcgccaacc acccaggaga gcggacgacg ttctttgccg atagattcct gcaacagcat
    15241 cagtgcctgc atgtacgctt caggacgcgg cgggcagccc gggatataca catcaaccgg
    15301 gatgaattta tcgacgccct gcacaacgga ataaatatcg tacataccac cagagttggc
    15361 acaggcaccc attgagataa cccattttgg ttccagcatc tggtcataca gacgctgaat
    15421 aaccggtgcc attttggtaa agcaggttcc tgcaaccacc atcaggtcag cctgacgcgg
    15481 cgaagcacgc aatacttctg cgccaaaacg cgccacgtca tgcaccgcgg taaacgaagt
    15541 caccatctca acgtaacagc aggaaagacc gaagttatac ggccaaattg agtttttacg
    15601 accccagtta accatgtcat tgagcttgcc cataaacacg tttttgttaa cttcttgctc
    15661 cagagggtcg gttacgatct cctgcttttg cagggggtaa cggtcgttct caccgttggg
    15721 atctatgcgg gtgagcgtat aatccatctt aatgcctcgc ggttagcgtt gacgattagc
    15781 gatactgttc gtttccgggt tcatacgctc gcggcgtgaa cgcgcgggcg tccagtccag
    15841 cgcgccaata cgcaccagat aaaccagacc tgccagtaac acaaaaataa aaattgcagc
    15901 ttccacaaag cctacccagc cgctttcgcg gatagaggtt gaccatgcga acagatacag
    15961 cgcttcaacg tcgaagataa cgaagaacat ggccaccaga taaaacttgg cggacaggcg
    16021 taagcgggcg gagccgaccg agtcgatacc ggattcaaac ggcacgtttt tcgacctcgc
    16081 gcgtgcgcga ccgcctaaaa accaaccgcc taccagcatc aggcaacaca ggccaatggc
    16141 aacgataaga aagatagcga atgcccagtg atgagcgatg acttcagtgg atgttgacat
    16201 actcattgct tactcatcaa aagtagcgcc agattcactg ctcttcacgg cagatggacg
    16261 ccacatcgat tcatggggag gaataaaaaa aaccttacaa taactgtaga aattctttta
    16321 tacagctaat tgatgtggtc ttttactcct ttctataacc ttttgtcaac tttaacaaaa
    16381 gcttcttcac attagtttac ataatatcaa caccattagc atttaatgcc ctttcacccc
    16441 agatccttga cgactccagg ataattagat gttgttgaat cgtgtccgtt gtgaagcaat
    16501 ggaaaaaata cgggtctatt ttgacaggaa tttgtgtcga ttcctccccc caaaagagag
    16561 tattttcttg atctgtgaca cgcttttgtt attccataac aaaaacgcag caacaaattt
    16621 acgtattttt taacatcatt gtagcaggtg atttttttca ggcgattatt tgtgcattcg
    16681 ggacgtgaat ctctggtgat tgaaaaataa acagttttgt acgttctgca ctatgcgagg
    16741 aaggattttt actaaaaaaa agccgctggg gtttaaaaca cccccagcgg ctcgtttttt
    16801 acactattgt ctcaggaatt atctatcgtc cgtcgattac tcgatatccc tttcaatcaa
    16861 caatgaatca tccccttccg gagcagacat tgggctgtat tgccacggat tatggtagct
    16921 ctccatcgcc tgatagatca cctgggccag ctcattatta ctggacgggt catagcacag
    16981 caaatattcg gtatcaggta gcggcggtaa gccatctact ccactcagca cacgcaggtc
    17041 cgggctcatc atctcaaccg gccttgccgt cacgccaaga ccggctttca ctgccgcacg
    17101 aacggccgga agcgtcgagg cgacataagc cagtcgccat ggaatatcta ctttattaag
    17161 cgtcgccagc accatatcgc gaaacggact tggatcatcc agcaatacaa gagggatcgg
    17221 ctcgcctttt tgcaatacgt attccgctgc gcagtaccag tgtgttggcg aggtacgcag
    17281 gttcaatgcc ttaaacgctg aaggacgatg ggtggttacc atcaaatcca cttcctggga
    17341 ttccagcatt tctgccatat aggcattacg tttaacgcgg acatccagcg ctaatttcgg
    17401 ataaaccgaa ctcacgcgat ttaacaggaa aggtaagatc gtatcggcag attcatctga
    17461 agcaccgata gttaacacgc cctgaagatt actgaacatt aatgatgagc aggcctcatc
    17521 attaaaacgc aggattttcc tggcgtaacc aagaagttga atgccatgtt cagttaacag
    17581 tttgttgcga ccgtgacgag cgaacagttc tttcccaacg agttgttcca gacgctgcat
    17641 ttgctgactt acggcggact gagtacgaca cacagcggca gctgcggcag caaaagtgtt
    17701 cagatcggca acagcaacaa atgttctcag cagatcgagg tcgaggttaa ttatcggacg
    17761 atttgcactt atcatatatt atcacttact ggcggctcat actgagctgg ttaatgctgt
    17821 gcacacacaa acaagcaatt ccatttgtaa tgtgcctccc tggcagt
//
LOCUS       DAZXUK010000060        17800 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZXUK010000060 DAZXUK010000000
VERSION     DAZXUK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17800
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.060"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..568
                     /locus_tag="ACH3L8_001637"
     CDS             <1..568
                     /locus_tag="ACH3L8_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4084798.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            526..1203
                     /gene="bglJ"
                     /locus_tag="ACH3L8_001638"
     CDS             526..1203
                     /gene="bglJ"
                     /locus_tag="ACH3L8_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFT4084799.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1241..2029)
                     /gene="fhuF"
                     /locus_tag="ACH3L8_001639"
     CDS             complement(1241..2029)
                     /gene="fhuF"
                     /locus_tag="ACH3L8_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFT4084800.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2170..2406
                     /locus_tag="ACH3L8_001640"
     CDS             2170..2406
                     /locus_tag="ACH3L8_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFT4084801.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2445..2531)
                     /locus_tag="ACH3L8_001641"
     tRNA            complement(2445..2531)
                     /locus_tag="ACH3L8_001641"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2495..2497),aa:Leu,seq:cag)
     gene            complement(2566..2652)
                     /locus_tag="ACH3L8_001642"
     tRNA            complement(2566..2652)
                     /locus_tag="ACH3L8_001642"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2616..2618),aa:Leu,seq:cag)
     gene            complement(2681..2767)
                     /locus_tag="ACH3L8_001643"
     tRNA            complement(2681..2767)
                     /locus_tag="ACH3L8_001643"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2731..2733),aa:Leu,seq:cag)
     gene            complement(3044..4075)
                     /gene="rsmC"
                     /locus_tag="ACH3L8_001644"
     CDS             complement(3044..4075)
                     /gene="rsmC"
                     /locus_tag="ACH3L8_001644"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFT4084802.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4178..4591
                     /gene="holD"
                     /locus_tag="ACH3L8_001645"
     CDS             4178..4591
                     /gene="holD"
                     /locus_tag="ACH3L8_001645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFT4084803.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4560..5006
                     /gene="rimI"
                     /locus_tag="ACH3L8_001646"
     CDS             4560..5006
                     /gene="rimI"
                     /locus_tag="ACH3L8_001646"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFT4084804.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5021..5698
                     /gene="yjjG"
                     /locus_tag="ACH3L8_001647"
     CDS             5021..5698
                     /gene="yjjG"
                     /locus_tag="ACH3L8_001647"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFT4084805.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5789..7378
                     /gene="prfC"
                     /locus_tag="ACH3L8_001648"
     CDS             5789..7378
                     /gene="prfC"
                     /locus_tag="ACH3L8_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFT4084806.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7771..8376
                     /gene="osmY"
                     /locus_tag="ACH3L8_001649"
     CDS             7771..8376
                     /gene="osmY"
                     /locus_tag="ACH3L8_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFT4084807.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8493..8654
                     /locus_tag="ACH3L8_001650"
     CDS             8493..8654
                     /locus_tag="ACH3L8_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFT4084808.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8776..9849
                     /locus_tag="ACH3L8_001651"
     CDS             8776..9849
                     /locus_tag="ACH3L8_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFT4084809.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9846..10628
                     /locus_tag="ACH3L8_001652"
     CDS             9846..10628
                     /locus_tag="ACH3L8_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFT4084810.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(10842..11705)
                     /locus_tag="ACH3L8_001653"
     CDS             complement(10842..11705)
                     /locus_tag="ACH3L8_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFT4084811.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(11677..13218)
                     /locus_tag="ACH3L8_001654"
     CDS             complement(11677..13218)
                     /locus_tag="ACH3L8_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFT4084812.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            13476..14255
                     /gene="deoC"
                     /locus_tag="ACH3L8_001655"
     CDS             13476..14255
                     /gene="deoC"
                     /locus_tag="ACH3L8_001655"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFT4084813.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14382..15704
                     /gene="deoA"
                     /locus_tag="ACH3L8_001656"
     CDS             14382..15704
                     /gene="deoA"
                     /locus_tag="ACH3L8_001656"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFT4084814.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            15756..16979
                     /gene="deoB"
                     /locus_tag="ACH3L8_001657"
     CDS             15756..16979
                     /gene="deoB"
                     /locus_tag="ACH3L8_001657"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFT4084815.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            17036..17755
                     /gene="deoD"
                     /locus_tag="ACH3L8_001658"
     CDS             17036..17755
                     /gene="deoD"
                     /locus_tag="ACH3L8_001658"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFT4084816.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         4263 a         4534 c         4797 g         4206 t
ORIGIN      
        1 attagtcatt gctgatttag ccggtgaaag tgaagatcca cgttctgttt gtgaacatta
       61 ttattcttta atctcacaat atcgggaaat tcactgggtt ttcatggtgt cacgctcctg
      121 gtattcccag gctgtagaac tgctcatgtg ccctacggcg acgttattgt ctgatgttga
      181 acccattgag aatctggtca agaccgtacg ttccggcaat acgcacgcag agcgtatcag
      241 cgccatgctg acctctccgg caatgactga aactcatgat tttagctatc gctccgtcat
      301 tctcactctt tcagagcgca aggtactgcg gctattaggt aaaggatggg gcatcaacca
      361 gatagcttca ttgcttaaga aaagtaataa aactatcagc gcccaaaaaa acagtgcgat
      421 gcgtcgactg gcaattcaca gcaacgctga aatgtatgca tggataaata gcgcgcaggg
      481 tgcaagagaa cttaacttgc cttctgttta tggagatgcc gcagaatgga acacagccga
      541 attaagaaga gaaatgtcgc actcatagaa aaatgcgtca tgagtagtat cggtattgag
      601 agtttattca gaaagtttgc gggcaaccct tataagctcc atacctatac cagtcaggag
      661 tcatttcagg atgccatgtc gcggatctcg tttgcggcgg tcattttttc tttttctgcc
      721 atgagaagtg agcgcagaga gggattatct tgcctgactg agctggcgat taagtttccg
      781 cgtacccggc gtttagttat tgtggatgat gatattgaag ctcggctgat tggttcattg
      841 tcgccatcac cgctggacgg tgtattaagt aaagcgtcaa cgctggagat ttttcatcag
      901 gaactctttt tgtcattaaa tggtgtacgt caggcgaccg accgactgaa caatcagtgg
      961 tacattaacc aaagccggac gttaagcccg acggagagag aaatattgcg ctttatgtcg
     1021 cgtggctact caatgacaca aattgccgag cagcttaaac gtaatatcaa aacgatccga
     1081 gcgcataaat ttaatgtgat gtcgaaactg ggcgtcagtt ctgatgcagg gttgttggag
     1141 gccgaagata ttctgttatg tatgcggcat tgcgaagcaa gtaatgtgtt gcatccctat
     1201 taatccgcat gatgccgggt ttacttcccc ggcagtgctt tcatttcagc gtacaatagc
     1261 cacattgctg cacatccggt aagcgataac gctggcagca agtgcggcgc accagcaggc
     1321 cgtcgcgcag taccacggta cgccagagtg gattatcttc accgttcgtg agcgttttct
     1381 caaaaaagag ggcatggcgc agcgattcaa cagtagcctc gccgagcagt tgcttcatct
     1441 cagtgagata ccagttgatc aaataaccgg tattactcca gataagtttg ccgttgatct
     1501 ctccggtcgc ttctagtgct tgcacaaccg gaaccagcgc ctggctgatt aacgtttcca
     1561 ttcgctgctg cggcgaatgt ggtgttgcgt ttttatcttc acacacatcg acccagaaac
     1621 aggcgacgcg tccggtttcg tgaaactcag catggaaatg ttccggcgac acatctaatg
     1681 ctttttcctg cgtcagtagc gccagcatta atggtggcac catcaggccg atataccatt
     1741 gtgcccatag tgagatcagc ggtttgttct cgcggatcat cgttggttgg ttgcgataga
     1801 tatgatcgga atagaccgcc agtagagaac ttagcgcatt cggtgatgac cattgcgcca
     1861 gcgtcatggc gttaagcgga gcgggttcat ccaggcggat aaactccagc aaatgttcac
     1921 gatgtttggc gatcatcgcc cgtacggctt gtgcaagccc agcatcctgc ggctggagat
     1981 gcgttcgcca gatgacatct tcatagagcg gtgcggaacg ataggccata atcgtgatag
     2041 taatctaaat gataatgatt gctaatccta gcgataggtt tactcgatag caagggattt
     2101 ctctggcttg caaatgataa aaattatcat atgatattgg ttttcattat caatgaaaga
     2161 gatgaaacca tgttgcaacg tacgctgggc agtggctggg gagtgttgct gccgggattg
     2221 ctgattgcag ggctgatgta tgcggattta tcgccggatc agtggcggat tgtcattctg
     2281 atgggattag tattgacgcc ggtgatgctg tatcacaaac agttgcggca ttacattttg
     2341 ctaccatcgt gcctggcgct tattgctggc atcatgctga tgataatgaa tttgaatcag
     2401 ggatgacaaa acagggaaga aacaagaaag gaagtaaaga taattggtgc gagggggggg
     2461 acttgaaccc ccacgtccgt aaggacacta acacctgaag ctagcgcgtc taccaattcc
     2521 gccaccttcg cacagtcatc ttactttttt tgatatcgcc tcgtttggtg cgaggggggg
     2581 gacttgaacc cccacgtccg taagaacact aacacctgaa gctagcgcgt ctaccaattc
     2641 cgccaccttc gcccagtgcg agcaatatca acgtgatttt tggtgcgagg ggggggactt
     2701 gaacccccac gtccgtaagg acactaacac ctgaagctag cgcgtctacc aattccgcca
     2761 ccttcgcata ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt
     2821 ttcagctttt cgtcaatagt taattatcgc ctgatgtgta attgctggaa aaatgtccat
     2881 caggaaacta gcgtgcaggt ttggtaggca tgcgggggca gatgccagat gcgacgctgg
     2941 cgcgtcttat ctggcctacg aagggctaat gtgcaggttt tgtaggtcgg ataaggcgtt
     3001 cacgccgcat ccgacacggt atgcggcgac agatatcaga tactcaacct ttcttcgcct
     3061 ggcgggtcat aatggcgcga tataccttga agcgcccggt ttgcgcgata acttcatgga
     3121 agccaaatgt ctcatccagc acgtccgggt aaggcaggaa ggcgttcgct acaattcgca
     3181 gctcgccgcc gctattaaga tgacgcaccg cgccacgaat cagcgtttgc gccgcatcca
     3241 ggctggtttg catcccatcg tggaacggcg ggttggagat gatcatatca aaacaacctt
     3301 tcacctcgga aaagacgttg ctggcaaaga cttcaccttc aacaccgttg gctgcaagtg
     3361 ttgcgcggct ggcttctacc gccggggcag agacatcgca caaggtgaga cgaattttcg
     3421 gcgaatggcg cgcaaaggca actgaaagta cacccgcgcc acagccgaca tccagcactt
     3481 tacctttcgt gtgcggagtt aacgtcgaga gcagcaactg gctaccgaca tccagaccgt
     3541 cgcggctaaa cacgccaggc agcgttttga ccgtcaggcc atcgacgctg tattcgcccc
     3601 agaatttatc cgcatcaaat actggctgtt tttccagacg accaaaatag aggccacagc
     3661 gacgagcgct gtcgacttta ttcaacggcg cataatctgc cagcatctgc tcagcgctgc
     3721 gcacgccgct gcggttctcg ccaacgacaa aaatatctgt ccccactggc agcagagaaa
     3781 gtaaattcat caactggaac tgggcttccg gtttgttctt cggccagtag taaatcagcg
     3841 tatcgcaatc tgcgacatca ttcgccgtgg cgaccagact aaaacgggcg ttatccccca
     3901 tctggcagct taacacctgc caatggtgga attgctgggt atgagcacgg ctggccgcgg
     3961 tatctaaacg cgcgggcagg tcatcctgta agtctccggc aaacagaata cggctttgtt
     4021 cgaaatcatc actgtgacgc agcaagactt cacttgccgg ggtaaatgca gacatggaat
     4081 gctcctcaat tgatactggc ggcgattata gccatatgtt ggcgcggtat cgacgaattt
     4141 gctatatttg cgcccctgac aacaggagcg attcgctatg acatcccgac gagactggca
     4201 gttacagcaa ctgggcatta cccagtggtc gctgcgtcgc cctggcgcgt tgcaggggga
     4261 gattgccatt gcgagcccgg cacacgtccg tctggtgatg gtggcaaacg atcttcccgc
     4321 cctgactgat cctttagtga gcgatgttct gcgcgcatta accgtcagcc ccgatcaagt
     4381 gctgcaactg acgccagaaa aaatcgcgat gctgccgcaa ggcagtcgct gcaacagctg
     4441 gcggttgggt actgacgaac cgctatcatt ggaaggcgct caggtggcat caccggcgct
     4501 caccgaatta cgggcaaacc caacggcacg cgccgcgtta tggcaacaaa tttgcacata
     4561 tgaacacgat ttcttccctc gaaacgactg atttaccagc ggcttaccac attgaacaac
     4621 gcgcccacgc ctttccgtgg agtgaaaaaa cctttgccag caaccagggc gagcgttatc
     4681 tcaactttca gttaacgcaa aacggcaaaa tggcggcgtt tgcgattacg caagtggtgc
     4741 tggatgaagc tacattgttc aatattgcgg tcgatcccga ctatcagcgt caggggctgg
     4801 gtagggcgct gctggaacat ctgatcgacg aactggaaaa acgcggcgtg gcgacactat
     4861 ggctggaagt ccgtgcctca aatgctgccg ccattgccct gtacgaaagt ttaggcttta
     4921 acgaggcgac gattcgccgc aattactacc ccaccacgga cggtcgcgaa gacgccatca
     4981 tcatggcgtt gccaatcagt atgtaagaca aggtggaata atgaagtggg actggatttt
     5041 ctttgatgcc gatgaaacgc tgtttacctt tgactcattc accggcctgc agcggatgtt
     5101 tcttgattac agcgtcacct ttaccgctga agattttcag gactatcagg ccgttaacaa
     5161 gccactgtgg gtggattatc aaaacggcgc gatcacttca ttacagcttc agcacgggcg
     5221 tttcgagagc tgggccgaac ggctgaaagt tgaagcaggc ttgcttaacg atgcctttat
     5281 taatgcgatg gcggaaatct gtacgccgtt gccgggcgcg gtttctctgc ttaacgccat
     5341 tcgtagcaac gctaaaatcg gcatcatcac caacggtttt agcgccttgc agcaagtgcg
     5401 tctggaacgg acgggcctgc gtgattactt cgatttgctg gtgatttccg aagaagttgg
     5461 cgttgccaaa ccgaataaga aaattttcga ttatgcgctg gaacaggcgg gcaatcctga
     5521 ccgttcacgc gtgctgatgg ttggcgacac tgccgagtcc gatattctcg gtggcatcaa
     5581 cgccgggctt gcgacctgct ggctgaatgc gcacaatcgc gagcaaccag aaggcatcgc
     5641 gcccacctgg accgtttctt cgttgcacga actggagcag ctcctgtgta aacactgatt
     5701 gcctcccccc cgttgatggg taaaatagcc gcaatttttc gttttcaaca agcgcggcgc
     5761 gatgccgctt actcaagaag aaagaattat gacgttgtct ccttatttga aagaggtggc
     5821 gaagcgccgc acttttgcca ttatttctca cccggacgcc ggtaaaacta ccatcaccga
     5881 gaaggtgctg ctgttcggac aggccattca gaccgccggt acagtaaaag gccgtggttc
     5941 caaccagcac gctaagtcgg actggatgga gatggaaaag cagcgtggga tctccattac
     6001 tacgtctgtg atgcagtttc cgtatcacga ttgcctggtt aacctgctcg acaccccggg
     6061 gcacgaagac ttctcggaag atacctatcg taccctgacg gcggtggact gctgcctgat
     6121 ggttatcgac gccgcaaaag gtgttgaaga tcgtacccgt aagctgatgg aagttacccg
     6181 tctgcgcgac acgccgatcc tcacctttat gaacaaactt gaccgtgata tccgcgaccc
     6241 gatggaactg ctcgatgaag ttgagaacga gctgaaaatt ggctgcgcac cgatcacttg
     6301 gccaattggc tgcggcaagc tgtttaaagg cgtttaccac ctttataaag atgaaaccta
     6361 tctctatcag agcggtaaag ggcacaccat tcaggaagtc cgcattgtta aagggctgaa
     6421 taacccggat ctcgatgctg cggttggtga agatctggca cagcagctgc gtgacgaact
     6481 ggaactggtt aaaggcgcgt ctaacgagtt cgacaaagag ctgttccttg cgggcgaaat
     6541 cactccggta ttcttcggta ctgcgctggg taacttcggc gttgatcata tgctgaatgg
     6601 cctggtggag tgggcgcctg cgccgatgcc gcgtcagacc gatacccgta ccgtagaagc
     6661 gagcgaagat aaatttaccg gcttcgtatt taaaattcag gccaacatgg atccgaaaca
     6721 ccgcgaccgc gtggcgttta tgcgtgtggt gtccggtaaa tatgaaaaag gcatgaaact
     6781 gcgccaggtg cgcactgcga aagatgtggt gatctccgac gcgctgacct ttatggcggg
     6841 tgaccgttcg cacgttgaag aagcgtatcc gggcgatatc ctcggcctgc acaaccacgg
     6901 caccattcag atcggcgaca cctttaccca gggtgagatg atgaagttca ccggtattcc
     6961 gaacttcgca ccagaactgt tccgtcgtat ccgcctgaaa gatccgctga agcaaaaaca
     7021 gctgctcaaa gggctggtac agctttccga agagggcgcg gtgcaggtgt tccgtccaat
     7081 ctccaacaac gatctgatcg ttggtgcggt tggtgtgctg cagtttgatg tggtggtagc
     7141 gcgcctgaag agcgaataca acgttgaagc agtgtatgag tcagtcaacg ttgccactgc
     7201 ccgctgggta gaatgtgcag acgcgaagaa attcgaagag ttcaagcgta agaacgaaag
     7261 ccaactggcg cttgatggcg gcgataacct cgcttacatc gctaccagca tggtcaacct
     7321 gcgcctggca caggaacgtt atccggacgt tcagttccac cagacccgcg agcattaatt
     7381 cttgcctcca gggcgcggta gccgctgcgc cctgtcaatt tcccttcctt attagccgct
     7441 tacggaatgt tcttaaaaca ttcacttttg cttatgtttt cgctgatatc ccgagcggtt
     7501 tcaaaattgt gatctatatt taacaaagtg atgacatttc tgatggcgtt aaataccgtt
     7561 caatgcgtag atattagtat ctaaagccgt cgattgtcat tctaccgata ttaataactg
     7621 attcagaggc tgtaatggtc gttattcatc acccatcgct tttgtgatgg cgaccattga
     7681 cttctgtaga gggtgaagtc tctccctatt cagcaatgca acctcgtgtt gctaggctca
     7741 aattacgagc aaacatacag gaataaatcg atgactatga caagactgaa gatttcgaaa
     7801 actctgctgg ctgtaatgtt gacctctgcc gtcgcgaccg gctctgccta cgcggaaaac
     7861 aacgcgcaga ctaccaatga aagcgcaggg caaaaagtcg atagctctat gaataaagtc
     7921 ggtaatttca tggatgacag cgccatcacc gcgaaagtga aggcggcgct ggtggatcat
     7981 gacaacatca agagcaccga tatctctgtc aaaaccgatc aaaaagtcgt gaccctgagc
     8041 ggtttcgttg aaagtcaggc ccaggccgaa gaggcagtga aagtggcgaa aggcgttgaa
     8101 ggcgtgacct ctgtcagcga caaactgcac gttcgcgacg ctaaagaagg ctcggtgaag
     8161 ggctacgcgg gtgacaccgc caccaccagt gaaatcaaag ccaaactgct ggcggacgat
     8221 atcgtccctt cacgtaaagt gaaagttgaa accaccgatg gcgtggttca gctctccggt
     8281 accgtagatt ctcaggcaca aagtgaccgt gctgaaagta tcgccaaagc ggtagatggt
     8341 gtgaaaagcg ttaaaaatga tctgaaaact aagtaattcg tcctcccgaa ctttgtcggg
     8401 aggcgcgatg tgcaccacac taaaaatatc gcattgagtg attttcacgc tcatatcaag
     8461 cggtgacatt aactatggta aaggagacgc ttatgtttcg ttggggcatc atatttctgg
     8521 ttatcgcgtt aatcgccgcc gcacttgggt ttggtggtct ggccggtacc gctgcaggcg
     8581 cagctaaaat tgtctttgtc gtcgggatta ttctgttcct agtgagtttg ttcatgggcc
     8641 gaaaacgacc ctagatttca gaccattaag aaaattttat ccaaagccag tccagcggac
     8701 tggcttttgc ggttttagcg aataataaat tgcgttactt tgtctttctt tcacaacaaa
     8761 aacaggaagg cagaagtggg gcagcgaata cctgtaacgc ttggtaatat tgcgccgttg
     8821 tcgctaaggc cgttccagcc tggacgaata gctctggtgt gcgaaggcgg cggacagcgt
     8881 ggaattttca cggctggcgt gctggatgag tttatgcgcg cgcagtttaa tcctttcgat
     8941 ctttatctcg gcacatctgc cggggcgcag aacctctcgg cgtttatctg caatcagccc
     9001 ggttacgcgc gcaaagtcat catgcgctat accacaaaac gcgaattttt cgatccattg
     9061 cgctttgtcc gtggaggaaa tcttatcgat ctcgactggc tggtggaggc caccgcaagc
     9121 cagatgccgt tgcaaatgga taccgccgcg cggttgtttg acagcggcaa atcgttttat
     9181 atgtgcgcct gtcgtcagga tgactacgcg ccgaattact ttttaccaac caaacaaaac
     9241 tggctggatg tgattcgcgc ctccagtgcg atacctggct tttatcgtag cggagtgtcg
     9301 ctggaaggca ttaactacct ggatggcggg atcagtgatg cgattccggt taaagaggcg
     9361 gcaaggcagg gcgctaaaac gttggtcgtc atccgcactg tgccgtcaca aatgtactac
     9421 acgccgcagt ggttcaaacg catggaacgc tggctgggtg acagtagcct gcagccgctg
     9481 gtcaatctgg tgcagcatca tgaaaccagc tatcgtgaca ttcagcaatt tattgagaaa
     9541 ccaccgggca agctgcggat atccgaaatt tatccgccga agccattaca tagtatcgcg
     9601 cttggcagtc ggattccggc gctgcgtgaa gactataaac ttgggcgttt atgcggtcgt
     9661 tatttcctcg ccacggttgg caagctatta actgaaaaag cgccgcttac ccgccatctg
     9721 gtgccagtgg tgacgccgga atcgattgtc attccgcctg cgccagtcgc caacgatacg
     9781 ctggttgccg aagtgagcga cgctccgcag gcgaacgacc cgacatttaa caatgaggat
     9841 ctggcttgat ttgccgtttt atcgacactc actgccattt tgatttcccg ccttttattg
     9901 gcgatgaaga ggccagcctg caacgcgcgg cacaagcggg cgtaggcaag atcattgttc
     9961 cggcgacaga ggcggaaaat tttgcccgtg tgcaggcgtt agcggaaaat tatcaaccgc
    10021 tgtatgccgt attgggcttg catcctggta tgttggaaaa acatagcgat gtgtctcttg
    10081 agcagctaca gcaggcgctg gaaaggcgtc cggcgaaggt ggtggcggtg ggggagatcg
    10141 gtctggatct cttcggcgac gatccgcaat ttgagaggca gcagtggtta ctcgacgaac
    10201 aactgaaact ggcgaaacgc tacgatctgc cggtgatcct gcattcacgg cgcacgcacg
    10261 acaaactggc gatgcatctt aaacgccacg atttgccgcg tgctggtgtt gtccacggat
    10321 tttctggcag ccagcaacag gctgaacggt ttgtacagct gggctacaaa atcggcgtag
    10381 gcggtactat cacctatcca cgcgccagta aaacccgcga tgtcatcgca aaattaccgc
    10441 tggcatcgtt gttgctggaa accgatgcgc cggatatgcc gctcaacggt tttcaggggc
    10501 agcctaaccg cccggaacag gctgcccgtg tgtttgccgt gctttgcgag ttacgcccgg
    10561 aacccgcgga tgagattgcc gaagtgttgc ttaataacac gtatgcggtg tttaacgttc
    10621 gtgggtagtt tgcccttcgt aggcagcatc cggcagtcgg cgcataatgc ctgatgcgac
    10681 gcttaacgcg tcttatcatg cctacaggtt tgtgccgaac cgtaggccgg ataaggcgtt
    10741 cacgccgcat ccggcagttg gcgcacaatg cctgatgcga cgcttgacgc gtcttatcag
    10801 gcctacaagt ctgtgccgaa ccgtaggccg tatccggcat gtcacaaata gagcgccgga
    10861 aatatcaacc ggctcacccc gcgcaccttt aatgcatcag ccaacggctc aacgtcttcc
    10921 ggcgtggcgc tcgcccagct ttgcgcctcg ccatacacgc cgtgggcatg aaacgcgttc
    10981 aggcgaaccg gaacatcacc aagtcccttg ataaacgccg ccagttcttc gatgtgttgc
    11041 aaataatcca cctggcctgg gatcatcagc aaacgcagtt ccgccagctt gccgcgctct
    11101 gccagcaaat agatgctgcg cttaatctgt tgattatcgc gtccggttaa ttgttgatga
    11161 cattcgctac cccatgcttt gagatcgagc attgcgccgt cacacaccgg gagtaatttt
    11221 tcccagccgg tttcgctcaa cataccgtta ctgtccacca gacaggtgag atggcgcagt
    11281 tgcggatcgt ttttgatagc agtaaacagc gccactacaa acggcagttg ggtcgtggct
    11341 tcgccgccgc ttaccgttat cccttcaata aacagcaccg ctttgcgcac atggctaagc
    11401 acttcgtcca cgctcatgga ttgcgccatt ggtgtggcat gttgcggaca catcttcagg
    11461 caggtatcac attgctcgca gaccacagcg ttccacacca ctttgccgtc aacaatctgc
    11521 aacgcctgat gcggacactg tggcacgcac tccccacagt cattgcaacg tcccatcgtc
    11581 cacggattgt gacagttttt gcagcgcaga ttgcagccct gcaaaaacag agccagacga
    11641 ctgcctggcc cgtcaacgca ggagaagggg ataatcttac tgactaaagc gcatctgctg
    11701 ttcatggctt atcacgcgcg gctggcgttc cagaatacga gtgttgcgtg cggcttcttc
    11761 gcccagccag gtggtgttgg tgcgtgaacc ttcggcgcga tatttttcta aatccgacaa
    11821 acgcaccata taaccggtaa cgcgaaccag atcgttaccg ctgacattgg cggtaaattc
    11881 acgcattccg gctttaaagg caccgaggca aagctgtacc agtgcctgcg ggttacgttt
    11941 gatggtttcg tcgagcgtca gaatgtcgct gatgccggaa taataataag catgatgcgg
    12001 ggcgacagtt tgcagatggg tgatcggatc tggctcatcg ccatacggca aacgcgcgcc
    12061 cggcgtggtg ccgatatcgg aactgatccc cgactgtgcg tgtaacatgg cgcgtttttg
    12121 ccagccatat ttcacggggg tattggcgac aaactccgcc agttgcgcgc tgatgcgata
    12181 acccacttca tttgcggcgg cttctttacc gtagcgcgcg gcaatccctt ctttctcaca
    12241 cagcaagtta accgcttccg ccagcccata catgccaaac attggcacaa aacgttcagg
    12301 gttaatcagc ccttctttca ccaggaagct attctcaaag aagtgtgatt gttgatagag
    12361 gaattcacac cgcgcatcga tgatggcgat ctgctgctgg cagtagtgcg gtagagtgcg
    12421 cgtaaagaag tcatccagcg attcgctgcg ctcggcaatg gctttcaggt taaggcgtac
    12481 cagcgtgctg ccaccacccg ccagcggcag tgagttgtaa cagctcacaa tcccgtagcc
    12541 cccttttgtg aaaattttat catgcaccgg accgttggcg atgtgcggtt tgctacattc
    12601 acagatgttc ttcgccactt ccagcagcag gtcatcaggg gtgatttcag gatcgtagat
    12661 aaaggtcagg ttcggtgaaa cctgcgcgtc tgcacgtaag atcgcgcggg taatgggcga
    12721 atcagacggg ccgatattgg cgtgcataaa ggcatctggc agggttctgt cgaggtaacg
    12781 ccagaaacgt tttattcgaa catcgatctc gttttgtgtt agaattctaa catacggttg
    12841 caacaacgca tccagttgcc ccaggtagac cggcatcgat gtgaccgacg gtacgtggtg
    12901 gtaaagaatg gtcagcagag agagtgcgtc atcaagatct ttcgcgcctt ccagctccag
    12961 ccattcggaa ccgttcgcca gaaaacgagc gtaatcgggt aagacatagc gcggtttgta
    13021 cggcgcatga ccttcaaaca tatcgcagat tacaccttca tccagcgcgc ggcgggcttc
    13081 ggcaggaagc tgtgggtaag gcagattgtt ttctgcttcc agtgccagaa aatggcgctt
    13141 ctgctccggg ctaagcactg ggctggtgac aatttgctgg caacgttgtt gcagtgcatt
    13201 ttcatgagaa gtgggcatct tcttttcctt ttatgccgaa ggtgatgcgc cattgtaaga
    13261 agtttcgtga tgttcacttt gatcctgatg cgtttgccac cactgacgca ttcatttgaa
    13321 agtgaattat ttgaaccaga tcgcattaca gtgatgcaaa cttgtaagta gatttcctta
    13381 attgtgatgt gtatcgaagt gtgttgcgga gtagatgtta gaatactaac aaactcgcaa
    13441 ggtgaatttt attggcgaca agccaggaga atgaaatgac tgatctgaaa gcaagcagcc
    13501 tgcgtgcact gaaattgatg gacctgacca ccctgaatga cgacgacacc gacgagaaag
    13561 taattgctct gtgtcatcag gccaaaactc cggtcggcaa taccgccgct atctgtatct
    13621 atcctcgttt tatcccgatt gctcgcaaaa ctctgaaaga gcagggcacc ccggaaatcc
    13681 gtatcgctac ggtaaccaac ttcccacacg gtaacgacga catcgaaatc gcgctggcag
    13741 aaacccgtgc ggcaatcgcc tacggtgctg atgaagttga cgtggtgttc ccgtaccgcg
    13801 cgctgatggc gggtgacgag caggttggtt ttgacctggt gaaagcctgt aaagaggctt
    13861 gcgcggcagc gaatgtactg ctgaaagtga tcatcgaaac cggcgaactg aaagacgaag
    13921 cgctgatccg taaagcgtct gaaatctcca tcaaagcggg tgcggacttc atcaaaacct
    13981 ctaccggtaa agtggctgtg aacgcgacgc cggaaagcgc tcgcatcatg atggaagtga
    14041 tccgtgatat gggcgtagaa aaaaccgttg gtttcaaacc ggcgggcggc gtgcgtactg
    14101 cggaagatgc gcagaaatat ctcgccatcg cagatgaact gttcggtgct gactgggctg
    14161 atgcgcgtca ctaccgcttt ggtgcttcca gcctgctggc aagcctgctg aaagcgctgg
    14221 gccacggcga cggtaagagc gccagcagct actaagtaag atgctttacg cctgatgcgc
    14281 tgcgcttatc aggcctacga gacgtatcta cccgtaggcc ggataaggcg tagacgcatc
    14341 cggcaaaagc cgcctcatac tcttttcctc gggaggttac cttgtttctc gcacaagaaa
    14401 ttattcgtaa aaaacgtgat ggtcatgcgc tgagcgatga agaaattcgt ttctttatca
    14461 acggtattcg cgacaacact atttccgaag ggcagattgc cgccctcgcg atgaccattt
    14521 tcttccacga tatgacaatg cctgagcgtg tctcgctgac catggcgatg cgagattcag
    14581 gaaccgttct cgactggaaa agcctgcatc tgaatggccc gattgttgat aaacactcga
    14641 ccggcggcgt cggcgatgtc acttcgctga tgttggggcc gatggtcgca gcctgcggcg
    14701 gctatattcc gatgatctcc ggtcgcggcc tcggtcatac tggcggtacg ctcgacaaac
    14761 tggaatccat ccctggcttc gacattttcc cggatgacaa ccgtttccgc gaaattatta
    14821 aagacgtcgg cgtggcgatt atcggtcaga ccagttcact ggctccggct gataaacgtt
    14881 tctacgcgac ccgtgatatt accgcaaccg tggactccat cccgctgatc actgcctcga
    14941 tcctggcgaa gaaactggcg gaaggtctgg acgcgctggt gatggacgtg aaagtgggta
    15001 gcggcgcgtt tatgccgacc tacgaactct ctgaagccct tgccgaagcg attgttggcg
    15061 tggctaacgg cgctggcgtg cgcaccaccg cgctgctcac cgacatgaat caggtactgg
    15121 cctccagtgc aggtaacgcg gttgaagttc gtgaagcggt gcagttcctg acgggtgagt
    15181 atcgtaaccc gcgtctgttt gatgtcacga tggcgctgtg cgtggagatg ttgatctccg
    15241 gcaaactggc gaaagatgac gccgaagcgc gcgcgaaatt gcaggcggtg ctggacaacg
    15301 gtaaagcggc agaagtcttt ggtcgtatgg tagcggcaca aaaaggcccg accgacttcg
    15361 ttgagaacta cgcgaagtat ctgccgacag cgatgctgac gaaagcagtc tatgctgata
    15421 ccgaaggttt tgtcagtgaa atggataccc gcgcgctggg gatggcagta gttgcaatgg
    15481 gcggcggtcg tcgtcaggca tctgacacca tcgattacag cgtcggcttt actgatatgg
    15541 cgcgtctggg cgaccaggta gacggtcagc gtccgctggc ggttatcctc gcgaaagacg
    15601 aaaacagctg gcaggaagcg gcgaaagcgg tgaaagcggc aattaaactt gccgataacg
    15661 caccggaaag cacaccaact gtctatcgcc gtatcagcga ataacggtat actgatctga
    15721 tcatttaaat ttgaagcact gagtacggag aacatatgaa acgtgcattt attatggtgc
    15781 tggactcatt cggcatcggc gctacagaag atgcagaacg ctttggtgac gtcggggctg
    15841 acaccctggg tcatatcgca gaagcttgtg ccaaaggcga agctgataat ggtcgtaaag
    15901 ggccgctcaa tctgccaaat ctgacccgtc tggggctggc gaaagcacac gaaggttcta
    15961 ccggtttcat tccggcggga atggacggca acgctgaagt tatcggcgcg tacgcatggg
    16021 cgcacgaaat gtcatccggt aaagataccc cgtctggtca ctgggaaatc gccggtgtcc
    16081 cggttctgtt tgagtgggga tatttctccg atcacgaaaa cagcttcccg caagagctgc
    16141 tggataaact ggtcgaacgc gctaatctgc cgggttacct cggtaactgc cactcttccg
    16201 gtacggtcat tctggatcaa ctgggcgaag agcacatgaa aaccggcaag ccgattttct
    16261 atacctccgc tgactccgtg ttccagattg cctgccatga agaaactttc ggtctggata
    16321 aactctacga actgtgcgaa atcgcccgtg aagagctgac caacggcggc tacaatatcg
    16381 gtcgtgttat cgctcgtccg tttatcggtg acaaagctgg taacttccag cgtaccggta
    16441 accgtcacga cttggctgtt gagccgccag caccgaccgt gctgcagaaa ctggttgatg
    16501 aaaaacacgg ccaggtggtt tctgtcggta aaattgcaga catctacgcc aactgcggta
    16561 tcaccaaaaa agtgaaagcg actggcttgg acgcgctgtt tgacgccacc atcaaagaga
    16621 tgaaagaagc gggtgataac accatcgtct tcaccaactt cgttgacttc gactcttcct
    16681 ggggccaccg ccgcgacgtc gccggttatg ctgcgggtct ggaactgttc gaccgccgtc
    16741 tgccggagct gatgtctctg ctgcgcgatg acgacatcct gatcctcacc gctgaccacg
    16801 gttgcgatcc gacctggacc ggtactgacc acacgcgtga acacattccg gtactggtat
    16861 atggcccgaa agtaaaaccg ggctcactgg gtcaccgtga aaccttcgcg gatatcggcc
    16921 agactctggc aaaatatttt ggtacttctg atatggaata tggcaaagcc atgttctgat
    16981 ggatttgggc ggagcgttga ctccgccttt gttatgtcac aaaaaggata aaacaatggc
    17041 taccccacac attaatgcag aaatgggcga tttcgctgac gtagttttga tgccaggcga
    17101 cccgctgcgt gcgaagtata ttgctgaaac tttccttgaa gatgcccgtg aagtgaacaa
    17161 cgttcgcggt atgctgggct tcactggtac ttacaaaggc cgcaaaattt ccgtaatggg
    17221 tcacggtatg ggtatcccgt cctgctccat ctacaccaaa gaactgatca ccgatttcgg
    17281 cgtgaagaaa attatccgcg ttggttcctg tggcgcagtt ctgccgcacg taaaactgcg
    17341 cgacgtcgtt atcggtatgg gggcctgcac cgattccaaa gttaaccgca tccgttttaa
    17401 agaccatgac tttgccgcta tcgctgactt tgacatggtg cgtaacgcag tagatgcagc
    17461 taaagcactg ggcgttgatg ctcgcgtggg taacctgttc tccgctgacc tgttctactc
    17521 tccggacggc gaaatgttcg acgtgatgga aaaatacggc atcctcggcg tggaaatgga
    17581 agcggctggt atctacggcg tggctgcaga gtttggcgcg aaagccctga ccatctgcac
    17641 cgtgtctgac cacatccgca ctcacgagca gaccactgcc gctgagcgtc agaccacctt
    17701 caacgacatg atcaaaatcg cactggaatc cgttctgctg ggcgataaag agtaattgtg
    17761 tttcgctgaa aggcgattgt cgtgttaagc cggagtgggg
//
LOCUS       DAZXUK010000061        17753 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZXUK010000061 DAZXUK010000000
VERSION     DAZXUK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17753
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.061"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(143..283)
                     /gene="yjjY"
                     /locus_tag="ACH3L8_001659"
     CDS             complement(143..283)
                     /gene="yjjY"
                     /locus_tag="ACH3L8_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFT4084817.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            379..1095
                     /gene="arcA"
                     /locus_tag="ACH3L8_001660"
     CDS             379..1095
                     /gene="arcA"
                     /locus_tag="ACH3L8_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFT4084818.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1154..2506)
                     /gene="creD"
                     /locus_tag="ACH3L8_001661"
     CDS             complement(1154..2506)
                     /gene="creD"
                     /locus_tag="ACH3L8_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFT4084819.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2564..3988)
                     /gene="creC"
                     /locus_tag="ACH3L8_001662"
     CDS             complement(2564..3988)
                     /gene="creC"
                     /locus_tag="ACH3L8_001662"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFT4084820.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3988..4677)
                     /gene="creB"
                     /locus_tag="ACH3L8_001663"
     CDS             complement(3988..4677)
                     /gene="creB"
                     /locus_tag="ACH3L8_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFT4084821.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4690..5163)
                     /gene="creA"
                     /locus_tag="ACH3L8_001664"
     CDS             complement(4690..5163)
                     /gene="creA"
                     /locus_tag="ACH3L8_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFT4084822.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5374..6243
                     /gene="robA"
                     /locus_tag="ACH3L8_001665"
     CDS             5374..6243
                     /gene="robA"
                     /locus_tag="ACH3L8_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFT4084823.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6240..6887)
                     /gene="gpmB"
                     /locus_tag="ACH3L8_001666"
     CDS             complement(6240..6887)
                     /gene="gpmB"
                     /locus_tag="ACH3L8_001666"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFT4084824.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6939..7459
                     /gene="yjjX"
                     /locus_tag="ACH3L8_001667"
                     /pseudo
     CDS             6939..7459
                     /gene="yjjX"
                     /locus_tag="ACH3L8_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(7544..7870)
                     /gene="trpR"
                     /locus_tag="ACH3L8_001668"
     CDS             complement(7544..7870)
                     /gene="trpR"
                     /locus_tag="ACH3L8_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFT4084825.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7960..9897)
                     /gene="sltY"
                     /locus_tag="ACH3L8_001669"
     CDS             complement(7960..9897)
                     /gene="sltY"
                     /locus_tag="ACH3L8_001669"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFT4084826.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10108..11775
                     /gene="ettA"
                     /locus_tag="ACH3L8_001670"
     CDS             10108..11775
                     /gene="ettA"
                     /locus_tag="ACH3L8_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFT4084827.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11882..12049)
                     /gene="nadS"
                     /locus_tag="ACH3L8_001671"
     CDS             complement(11882..12049)
                     /gene="nadS"
                     /locus_tag="ACH3L8_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFT4084828.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(12083..13315)
                     /gene="nadR"
                     /locus_tag="ACH3L8_001672"
     CDS             complement(12083..13315)
                     /gene="nadR"
                     /locus_tag="ACH3L8_001672"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFT4084829.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(13336..14718)
                     /gene="radA"
                     /locus_tag="ACH3L8_001673"
     CDS             complement(13336..14718)
                     /gene="radA"
                     /locus_tag="ACH3L8_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFT4084830.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14767..15735)
                     /gene="serB"
                     /locus_tag="ACH3L8_001674"
     CDS             complement(14767..15735)
                     /gene="serB"
                     /locus_tag="ACH3L8_001674"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFT4084831.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15841..16485
                     /locus_tag="ACH3L8_001675"
     CDS             15841..16485
                     /locus_tag="ACH3L8_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFT4084832.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16513..17528
                     /gene="lplA"
                     /locus_tag="ACH3L8_001676"
                     /pseudo
     CDS             16513..17528
                     /gene="lplA"
                     /locus_tag="ACH3L8_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            17560..>17753
                     /locus_tag="ACH3L8_001677"
     CDS             17560..>17753
                     /locus_tag="ACH3L8_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFT4084833.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSK"
BASE COUNT         4229 a         4958 c         4475 g         4091 t
ORIGIN      
        1 aactagttgg attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat
       61 caaactgtta acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg
      121 gtgtccggcc cctcttagct tgttatgttg ctgttaaaat ggttaggatg acagccgttt
      181 ttgacactgt cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt
      241 tgatggaaag tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt
      301 aatttgcagc atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg
      361 gcaatttagg tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta
      421 acacgcaaca cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca
      481 gatggcgcgg aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat
      541 atcaatctgc cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat
      601 gttgcgttga tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa
      661 atcggtgcag atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca
      721 cgcaacctgc tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt
      781 gaaagctaca agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct
      841 gatggcgagc agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa
      901 aacccaggca aaattcagtc tcgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg
      961 aaaccgcacg accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct
     1021 acgccggata cgccggaaat catcgccacc attcacggtg aaggttatcg cttctgcggt
     1081 gatctggaag attaatcggc tttaccaccg tcaaaaaaac ggcgcttttt agcgccgttt
     1141 ttatttttca accttatttc cagatacgta attcatcgtc cgttgtaact tctttactgg
     1201 ctttcatttt cggcagtgaa aacgcatacc agtctatatt acgagtcaca aacatcatgc
     1261 cggccagcgc caccaccagc acactggttc ccaacaacag cgcgctatcg gcagagttga
     1321 gcagtcccca catcactcca tccagcaaca acagcgcgag ggtaaacaac atgctattgc
     1381 gccagccttt caataccgcc tgcaaataaa taccgttcat taacgcccca atcagactgg
     1441 cgattatcca tgccacggta aaaccgatat gttcagaaag cgccagcaag agcaaataaa
     1501 acatcaccaa tgaaagcccc accagcaaat attgcattgg gtgtaaacgt tgcgcggtga
     1561 gcgtttcaaa aacaaagaac gccataaaag tcagtgcaat cagcagaatg gcgtacttag
     1621 tcgcccggtc cgttaattgg tattgatcgg ctggcgtcgt cactgcgacg ctaaacgcgg
     1681 ggaagttttc ccagccggta tcattgcctg aagcaaaacg ctcaccgaga ttattagcaa
     1741 accagctgct ttgccattgc gcctgaaaac ctgactcgct agcttcccgt ttggctggta
     1801 gaaaatcacc taaaaaactg ggatgcggcc agttgctggt taaggtcatt tcgctattac
     1861 gcccggcagg caccacagaa agatcgccgg taccgcttaa attcagtgcc atattcagct
     1921 gcaggttctg cttccgccag tccccttcgg gtaaagggat atgcacgccc tgcccgcctt
     1981 gctctaaccc ggtaccgggt tcaatggtca gtgccgttcc gttaacttca ggcgctttca
     2041 ccacaccaat accacgcgca tccccgacgc taatcacaat aaagggcttg cctaagatga
     2101 tatttggcgc gttgagctcg ctaagacgcg aaacatcgaa atcggctttt agcgttaaat
     2161 cactgtgcca gacctgaccg gtataaatcc ctatcttgcg ttcttccacg ttctgattgc
     2221 catcaaccat caatgactca ggtaaccaaa aatggataaa acttcgtttc cgctccacgg
     2281 ttttatcctc ttcctgcacc gtataaagct cggtcacagg aatagcgata agcggcccaa
     2341 cgagtttttg cggcccgctg gtactttggc gaatcgcatc ttccacatcg ctacggtaat
     2401 cggcacgttc gacaatcact tgccgaatca gcattatcgg aatcaacaac agcaatactg
     2461 caccaaacaa ggtggtcatt ttccagaaca ggggggattt caacatagtc ttctcctttg
     2521 caatggcagc aggatacgaa ggctatgtgg gaagaatttg aagctatgtg aagtgacggt
     2581 gaagtcgtag cgaggccagc acgccacctt cctgcacgtt gcgcagcgtg acttcgccgt
     2641 taaacaaacg ggcgacctca ctgacgaacg ccaaccccag accgctgctt ttttgcccat
     2701 ttgcacgcgg caaagagtaa aagcgttcaa aaatacgtga cagcgcgtag tcaggaatac
     2761 cactaccggt atccagcacc ttaagcgtga cgtgttcctg atccacttcg gcgcttagcg
     2821 ttatgcaacc gctctcggga gtaaaatcga tggcgttatc cagtaaattc cccagcgcct
     2881 gatccagtaa cgccggttca gcagcaacat taacctcggt gggcataaca tgcaaagtga
     2941 tgtttttttc tgccaactgc acggtgcgcg cttcgctgac gcggcgaaat aatgccgcca
     3001 catcaacagc agtcagaacg acttcctgac gattctccag tcttgcctgg cgtagtaacg
     3061 tttccaccag cgcctgcatt cgcgcatttt gtgtcagaat gttgtcagta aaacgagcca
     3121 ctacttcagg cggcggacct tcgcgtaaaa tttccgccgc gccacgaatc gccgccagtg
     3181 ggctttttag ctcatgggtt aacgcataaa catactgctc aatatagttt ttcccttcca
     3241 gcttcacgcg catactttcc agcgcctgcg cgagtttacg cagctcgcta ctaccgagat
     3301 cgggaagagg aacgggctta ttgtcagtga cggaatcagc atagcgagtg agccgggcaa
     3361 tggagcggtt gatccaccaa accatgcctg cgccaatcac cagtgcaatc cccaacaaaa
     3421 tggcgctggc ccataaaatt cgccgctcgc tgcgtttaat gaccggagcc atcgccgcgt
     3481 tcggtttgcc tacgctcaaa acgccaataa gccgcgagcc gcccataatc ggcgcggcaa
     3541 catacatcac cgaactttcg ggatcggcag gattttgcaa cgtgctgcgc gcaccatact
     3601 gaccacgcaa cgttagccag acgtcattcc agcgcgaata atcctgtcca acggctttat
     3661 ttgccgaatc gaacaatact ttgccatgcg catcggtcat atagacatga tattcattgc
     3721 gcactttgtt aatgccacca atattggcgc gaaacgggcg atgttgtagc tgattaaacg
     3781 cctgcgccag ttgcccatgc gttgggtccc cagagagcaa atcgggacgc gccagctccg
     3841 caagtaacgt tgccgtgtcg atcaacgtcc cctccgttgc tcttcgcacg cccggtttaa
     3901 cttctttgac aaaaatagcc agtacgaacc aggccgccac cgccaccagt aaaaaatagc
     3961 ccagcagcaa ccgcatgccg atacgcatta caagcccctc aggctatatc ccatgccgcg
     4021 atgagtatta atcggtgaaa gatcggggtt gatggcgcgc agcttggcac gcagcgtttt
     4081 aatgtggata tcgacggtgc gatcgtaggt gtcctgcgca tcttcccaca cgctatccat
     4141 tagttgctgg cgggaccaga cgcggcctgg cgacttgagt aacgtcttca gcaataaaaa
     4201 ctcataccgc gtcagcgtta atggcgtgtc aaaccagctg atctgcgccg cgggttcatt
     4261 caattcaaaa tgaccaatac ggatgacggg agacggcgtc gagaacttct tcacccgacg
     4321 cagtaaggtg cgcaccctgg cgcacacttc gcggggtgaa aacggtttag ccacatagtc
     4381 gtcagcgcca atttccagcc caagcaggcg atcgacctct tcacttcggg ccgtcaggaa
     4441 cagtacaggt aacgccggat ggagcgccag taactggcgg cacaattcaa agccactgat
     4501 atcaggcaaa ccaacgtcga gaatcatgac gtcgggaacc tgctggcgag ctttatccag
     4561 caccggcaaa cctcgctcaa agacctcgac ggcaaaacct tcctgctgca acatgtagac
     4621 cagcgtgtcg gctatccctt gctcatcttc cactaaccag accgtttccc gttgcataaa
     4681 tcgcctctgt tattgccgcc acggcatgac aggaaccgcg ctaatcgcgt ttttcggcga
     4741 accttctaca actttgtcgg agtaagccag ataagcgagc gcgttgcgtt tggcgtcata
     4801 aaagcgcacg acctgtaacg acttaaagac cagggacgtg cgttttttga atactacctc
     4861 gccctgagct ttgccgtttt taatacgatc cgacagttca atcggcccga cttgctgaca
     4921 agaaatggcc gcatcggagg tatcttctgc caggcccaat ccccctttaa taccaccggt
     4981 tttcgcccgg ctcacataac aggtgacatt tttcacatcg ggatcgtcaa aggcttccac
     5041 aacaattttg tgatccgggc cgatcatttt aaatacggtg tcgaccgaac caatctcttc
     5101 tgcatgagcc aatggcccca gcattatcag gcttaaagac aggatcaaat gcttgtattt
     5161 catattgtta ccatttttat ctattacacg agcaattagt tcgtcacggt aaataacata
     5221 ttgattaaga acaaaatctc aattctttta tttccgtcaa gccctaaaac atactctact
     5281 aagaaaaaaa cactgaatgc taaaacagca aaaaatgcta ttatccaatt acctgatgtc
     5341 aggtgctcgt tgttgaaagg atgaggatat tttatggatc aggccggcat tattcgcgac
     5401 cttttaatct ggctggaagg tcatctggat cagcccctgt cgctcgacaa tgtagcggcg
     5461 aaagcaggtt attccaagtg gcacttacag agaatgttta aagatgtcac tggccatgct
     5521 attggcgcgt atattcgtgc tcgtcgtttg tcgaaatcgg cggtcgcact acgcctgact
     5581 gcgcgtccga ttctggacat cgcgctgcaa taccgcttcg actctcaaca gacatttacc
     5641 cgcgcattca agaagcagtt tgcccagact cctgcacttt accgccgttc tcctgaatgg
     5701 agcgcctttg gtattcgccc gccgctacgt ctgggtgaat tcacaatgcc agagcacaaa
     5761 tttgtcaccc tggaagatac gccgctgatt ggtgttaccc agagctactc ctgttcgctg
     5821 gagcaaatct ctgatttccg ccatgaaatg cgttatcagt tctggcacga ttttctcggc
     5881 aacgcgccga ccattccgcc ggtgctctac ggcctgaatg aaacgcgtcc gagtcaggat
     5941 aaagacgacg aacaagaggt attctatacc accgcgttag cccaggatca ggcagatggc
     6001 tatgtactga cggggcatcc ggtgatgctg cagggcggcg aatatgtgat gtttacctat
     6061 gaaggtctgg gaaccggcgt gcaggagttt atcctgacgg tatacggaac gtgcatgcca
     6121 atgctcaatc tgacgcgccg taaaggtcag gatattgagc gatactaccc ggcagaagac
     6181 gccaaagcgg gagatcgccc aattaatctg cgctgtgaac tgctgattcc tatccgccgt
     6241 taacgctgca gctcatctaa tgcaggggcg tccagatgcg agatgtcccc tgcggtttca
     6301 acgacccagc cagacgccag ccacaggctt tcctgataat ccacgcgcga aatagaacag
     6361 ttacgcagac gtaagcggcg ctctgcccat gctggtaatc cgagaatcgt actcaccaga
     6421 catcccagtg caataccatg gctcaccagc agcggtcggc ttccctgcgg taagtcccgg
     6481 caagattcca gtgccgcatt gacgcgatcg ctgagctcct gcattgactc gccttcagga
     6541 atacgcccgt caacggtgcc attgaccagc tgccgacgcc agttctcttc ttcttcggtc
     6601 agagaatcga tatgtctttt ttccagcaca cccatgttta attcacgcag gcgagaatca
     6661 aagatgatgt cacagccgca ggcctgggcg atgatttccg ccgtacgccg ggtgcgtcct
     6721 aaatcgctac tgataatatg cgtaatgcca agctctttgg cacgggttgc cacctgcatc
     6781 gcctgttgct cacctttggc ggtcagcggg ctgtcagact ggccctgaat acgtcgctcg
     6841 gcgttccact gcgtttcacc gtggcggact aggtatacct gtaacatgct ttttttccgt
     6901 atactgctgt aacgttaatt ttctgagtaa tgctgattat gcaccaagtt gtctgtgcga
     6961 ccaccaatcc cgctaaaatt caggccattc tgcaggcatt tcacgagatc ttcggcgaag
     7021 gatcctgcca tattgcatcc gttgccgtcg agagcggtgt accggaacaa ccctttggca
     7081 gtgaggaaac gcgcgctggc gcacgaaatc gggtagcaaa tgcccgccgt ttacttccag
     7141 aggctgattt ttgggtggcg attgaagctg gcattgatgg cgacagcact ttcagttggg
     7201 tggtgattga aaacgccagc cagcgcggcg aggcgcgttc ggcaactttg ccgttacctg
     7261 cggtgattct ggaaaaagtt cgtgaaggcg aggcgcttgg tcccgtgagt cgcgttatac
     7321 cggtattgat gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact
     7381 cactcgcgcc agcgtctatc accaggcggt gatcctggcg ctgagtccgt ttcacaatgc
     7441 cgtgtatcag gcattgtagg acggataagg cgttcacgcc gcatccgaca ttcggtgcac
     7501 gatgcctgat gcgacgctgc cgcgtcttat caggcctaca aaatcaatcg cttttcagca
     7561 acacctcttc cagccactga cgcagctcca cgggcgcggc tttcaggctg ttagatccac
     7621 gcgtaatcgt cgcgatgccc gcgccgagtt cattttttaa ctcacgctga ctcatttcgc
     7681 cacgcaacag ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg
     7741 tcagcatcag gtttaacaac ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt
     7801 cgacaaaacg taaccactcc tggtgacgct gttctgccat cgctgctgaa tagggtgatt
     7861 gttgggccat aatatgtcgc cattgttagc gggggaagca aaatgactcc cccggttgta
     7921 ctcgctaaag agtacgatag catatcataa acgtgcggat cagtaacgac gtccccattc
     7981 cgtggcgctc atcaacgtcg gtttatcccc catgaaatag cggtagtaag catcataagc
     8041 cagcacgttc ttcacataac cgcgcgtctc ggagaacgga atactctcga caaatgccac
     8101 agcgtcgata cgcccggcgc tgttgccaag ccaggttcgc acccgtccag gtccggcgtt
     8161 ataagctgct gaggagaaaa tacggttatt gccaaactgc tgataaacat attgcaggta
     8221 actggtgcca atgttgatgt tcgtttccgg atccagcaat tgcccagggc tgctataacc
     8281 aggaatagag aacatcttca ccgtatgggt cgctgtacca ggcataatct gcatcaggcc
     8341 gctggcccct accggtgatt tcactttcgg attccaggcg ctctcctgac gggcaatcgc
     8401 catcgcatag ctttgcggga tctctttccc actggtgtaa cgtttgaaaa gatcgttgta
     8461 agccagcggg aatcgctctt ccagatgatc ccaaagcttc ccggcgatcg ttgcctgaac
     8521 gctaagatcc caccattggt tattgaaagc ataccgagcc agttgagcct gctctgtttt
     8581 tgacttgctc ttcaccagat tggcccactc gctacgcgcg gtattatcga gattccagta
     8641 catcaactcg cgcacgcgcg ccatctccgg cccctgagtc agggtgctgt caacattctg
     8701 cggcgcttta tcaatcttca gctcatactc ttcaccgatg cgttgtgctg caaccatcgg
     8761 gtagaaacca cgctgttgta tgagttgatg caaaatctct ttcgcttcag cttcacgtcc
     8821 gcgttccagc aataaatccg cctgccagta acgccattca tctttctctt ttgcttccat
     8881 cggcagacgc gccagccagg tattcaggcc gcgacgatcg ccggtgccaa gcgccattcg
     8941 cacacggcgt tcaatcaacg acgtggattg cgagcgcata atggcgtcat cgcgccattt
     9001 cgcctgctca tcggtgacat cattgcccat caaacgccag gcgacgatat cgcgcagctc
     9061 ctgaatctga tcttcattaa gctgctgcgc ctgggcaagc gacgggatca tcagccgtgc
     9121 attttcggca tcctgccgcg ccacactggc aaacgccacc gccgccattt gacgggtaaa
     9181 atcggtcgcg ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga
     9241 aatgattgcc gaggcgatag tctggtaatc ggcaggcatc tgccctgcca gcactgttac
     9301 caggcctgtg ttacccgctt tcatcgccag acggatacgc tctaaatacg ccagcggatc
     9361 ttgtttacct gacgcacgcc agacgctaaa taacttgtca caggcgttag gctggctctt
     9421 gccggttagc cacagctctt tcgccccttg ccaggcttct tcactctgcc cggtgttcca
     9481 tttcgcatag tagtaattac attgcgcttc ggtagttccg ggcttttccg ggctaaaggc
     9541 taacaagcca cgccagtctt cacgccgcgc cagttcattg acgaaacgag attgcagcgt
     9601 gcgagcggga ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg
     9661 attcatcagg tcatcggtga tctggcggta ttccaggtag ggataaagcg gataatcctt
     9721 cagtccaggc atcatttgtt cgaccacatc catctgtcga ttatcccagg cctgcttgat
     9781 ttgcgcgtaa cggctacgct gctcatccag tgagtcggct cgcgccacgc tgctgaccgt
     9841 cagcagacag acaccggcag ccaacagccg ccaggtaact tgtttggctt tttccacaag
     9901 cgcatcctct aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag atttatgcta
     9961 accagtcatt gccgtttacg ccacgttacg gacacttttt tacttttact gcgaaggtga
    10021 tcgaccaact gggattaggc ggggaaacag gctacacttc gcctttgaaa actcatcatc
    10081 acgataaaca tagaggcgaa gtccaacgtg gctcaattcg tttataccat gcatcgtgtc
    10141 ggcaaagttg ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag tttcttccct
    10201 ggggcaaaaa ttggtgtcct gggtctgaac ggcgcgggta agtccaccct gctgcgcatt
    10261 atggcgggca ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga catcaagatt
    10321 ggttatctgc cgcaggaacc gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa
    10381 gaagcggttt cagaagtggt taacgccctg aaacgcctgg atgaagtgta tgcgctgtac
    10441 gccgatccgg atgccgattt tgacaagctg gccgctgaac aaggccgtct ggaagagatc
    10501 attcaggctc acgacggtca taacctgaac gtacagctgg agcgtgcggc ggatgcgctg
    10561 cgtctgccag attgggacgc gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgtg
    10621 gcgttgtgcc gcctgctgct ggaaaaacca gacatgctgc tgctcgacga accgaccaac
    10681 cacctggatg ccgaatccgt ggcgtggctg gaacgcttcc tgcacgactt cgaaggcacc
    10741 gtggtggcga ttactcacga ccgttacttc ctcgataacg ttgcaggctg gatcctcgaa
    10801 ctggaccgcg gtgaaggaat tccgtgggaa ggtaactact cctcctggct ggagcagaaa
    10861 gatcagcgtc tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa
    10921 gagctggagt gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg
    10981 gcacgctttg aagagctgaa cagcaccgaa taccagaaac gtaacgaaac caacgaactg
    11041 tttattccac ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa
    11101 tcctacggcg atcgcctgct gattgatgac ctgagcttct cgatcccgaa aggggcgatc
    11161 gtcgggatca tcggtccgaa cggcgcgggt aaatcgaccc tgttccgtat gatctccggt
    11221 caggaacagc cggacagcgg caccatcact ttgggtgaaa cggtgaaact ggcatcggtt
    11281 gatcagttcc gtgactcaat ggataacagc aaaaccgttt gggaagaagt ttccggcggg
    11341 ctggatatta tgaagatcgg caacaccgag atgccaagcc gcgcctacgt tggccgcttt
    11401 aactttaaag gggttgatca gggtaaacgt gttggcgaac tttccggtgg tgagcgcggt
    11461 cgtctgcatc tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca
    11521 accaacgacc tggatatcga aaccctgcgc gcgctggaaa acgccctgct ggagttcccg
    11581 ggctgtgcga tggttatctc gcacgaccgt tggttcctcg accgtatcgc cacgcacatc
    11641 ctggactacc aggatgaagg taaagttgag ttcttcgaag gtaactttac tgagtacgaa
    11701 gagtacaaaa aacgcacgct gggcgcagac gcgctggagc cgaagcgtat caagtacaag
    11761 cgtattgcga agtaatgcgt aaaatgccgg atgcggcgcg aacgccttat ccggcctaca
    11821 aaaccagcaa tttcaatacg ttgtaaaact gtaggcctga taagacgcgg taagcgtcgc
    11881 atcaggcatt gagcaccgaa tgccggatgc ggcgtgaaca ccttttccgg tctacaacag
    11941 ccttagcatc ctcaatttcg tggcgggtca cccgtgcagg ttttttcaaa ccttgtttaa
    12001 tctcgacagc ctcttccaga gaggttttca actcatcaaa aaaactcatt ttatacctcg
    12061 cctttgagca gtttcgtcac ggttatcctt gctcccccat catctcccgc accagctcca
    12121 cgcagcgcag gaaacggctg tcgtaatcgt cctcttcaac ccgcacgaat tcgatattgt
    12181 tctcttccag catctccacc agcaagttct ggaactcttt gcgatccacc gaactgccga
    12241 ggctgcgtaa accatccgcc acccacggcg tgttgttctc cagcaggatc accagatcga
    12301 aacggtattc atcaatcagc gcctgcacga acggatgctc acgcccttcg tactttttgc
    12361 agaacgcctg ggtagtgaca aaatcggtgt caataaacgc cactttattg gcgtatttca
    12421 ctgcaaaatc aatgtattgt gcgtgaccca acgcgatttt atcgtagtcg gaatactgca
    12481 atgcgatctc atcaccgccg aggtgtgaaa agacataatc gcggccatat tcccacgcac
    12541 tggtggtgtt gaagatattg gcaagtttgt ttaccagggt ggatttaccg ctcgactcgc
    12601 cgccaaggat cgccacggta cgcacaaaaa acggcttcac ttcggtagga atatattccc
    12661 agtagcggaa cgggttttcg cggatctgcg caccgctgat actcataaag gtacgtttcg
    12721 gatcgaccag caccgtctcg atccccagat gttccatata ctgtggcgca tcggcttctt
    12781 ccgaggtgta gatcaggtcc ggctggatcc ccttttcagc cataaacttt ttgatgccgt
    12841 tgctccacac atcccagcca tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa
    12901 tacggatatt tttctgatat ttaaaggttt gcaatagcca gcgcagacga tccggcaccg
    12961 tgggctgctg cgacatggcg ctgtcttcaa acaacgcgcg gtcgcgggta tcgtcaaaac
    13021 ccataatgat atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc
    13081 cggtatgcag cgggtagaac ttaccgaata cgacaccaat ggttttcttc tgccggggaa
    13141 attcaagccc caaaaaacgg tgcaatgcct ccagcttttg cgcgctgggg cttttaattt
    13201 tggcattcag taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct
    13261 gtagcgtgca gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc
    13321 cctccgtatc tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac
    13381 gccaaaaatc tgcatccctt ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc
    13441 ccggcgaaaa ccgtgtttcg ccgcttcaga aatccgctcc tggccactgg gcaccgggcg
    13501 gatctcccct gccagcccga cttcaccaaa caccaccaga tcctgtggca gcggtctgtc
    13561 acgcaggctg gaaaccatcg ccagcagtaa cgctaagtcg gcgctggttt cggttacctt
    13621 cacgccgccg accacgttca caaacacatc ctgatcggcc atttgcagac caccgtgacg
    13681 gtgcaagaca gccagcagga ttgccagacg gttttgttcc agccccactg ccacgcggcg
    13741 cggattcgcc attatcgagt gatcgaccag cgcctgaatc tccaccagca acggacgcgt
    13801 tccttcccac accaccatca cggagctgcc ggaggtcact tcatctccgc gacttaagaa
    13861 aattgccgaa gggttgctga cttcacgcag cccctgttcg gtcatcgcga agacgcccag
    13921 ctcattcacc gcgccgaagc ggtttttatg gctgcgtaag gtgcgaaaac gggagtcggc
    13981 atcgccatcc agaagcaccg aacagtcgat acagtgttcc agcactttcg ggccagccag
    14041 cgaaccatct ttggttacgt gtccaaccat gacaatcgcc acaccgcgcg ttttggcgaa
    14101 acgcgtcaga taagccgccg tttcacgcac ctgcgccacg ctaccaggcg atgattgtac
    14161 atccgccata tgcatcacct ggatcgagtc aattaccatc agcttcggtt gctcttcttc
    14221 ggcaatcagg cagatctgct cgatgctggt ttccgacaac atattgagat tgtcagtcgg
    14281 caggccaagg cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cggtgacata
    14341 cagcgttttc atctgctggg ccagtttgca cagcgtttgc agcaacagcg tggatttccc
    14401 cgcaccaggg ttaccgccaa tcagaatggc acttcctggc accacgccgc ctcctagtac
    14461 gcggtcgaac tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga
    14521 gagtttctgg acttttgcca ccccggcgct accggcatag ccgctgagac gctcgttacg
    14581 cgccaccgtt ggcgacgcag caagacgcac ctcggtgatg gtgttccagg catgacaggc
    14641 actgcactgc ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg
    14701 ttttggagct tttgccacgg tgtacctcgt taatgctgtg ccgcccgcag gatgacgggc
    14761 aggcaattac ttctgattca ggctgccaga gaggatgcag aataccccca tcaggtcagc
    14821 gtgacggatg gtgacttccg ccttttcatt cacttttggc ttggcatggt aggcaatccc
    14881 cagccctgct gctttgatca tcggcaggtc attagctcca tcgccaatcg ccacggtctg
    14941 cgccagcggg atttcatact cctgcgcgag gcgagtcaga gttttcgctt tgtactgcgc
    15001 gtctacgatg tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt
    15061 ggctaccacg gctgtcaggc gcagcttgtc gcgcaggtat tcagcaaaga aagtaaagcc
    15121 gccggaggca atcgccactt tccagcccag cgtttccagc ttgagcacca gctgcgttaa
    15181 gcctggcatc agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt
    15241 cagcgtcgcc acgcggctgc gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg
    15301 ttcggttacc tccgccacca tctcgcccgt tccggccagc ttggcaattt catcaataca
    15361 ttcaatctgg atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg
    15421 cgggattttc cccagcgggg cgacatccag ctgcgcttcg tgggccaggc gtgtagcccg
    15481 tgcggtgagt gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac
    15541 aatcaccatc gccgcaccca gtttgctctg gtattgggtc agacgctgtt tatccagccc
    15601 acgaccatac agcagccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc
    15661 accacttaat gaaagaggca gacccggcca taaagagaca tcttcaggca ggtcgcacca
    15721 ggtaatgtta ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga
    15781 ggctaccttg tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag
    15841 atggctcgca caaaactgaa attccggcta catcgggcag tgattgtcct gttctgtctt
    15901 gccttgttag tggcactgat gcagggggcg tcatggttta gtcaaaacca ccagcgacag
    15961 cgtaatccac agctggaaga actggcccgc accctggcgc gtcaggtgac gctgaacgtt
    16021 gcaccgctga tgcgtaccga ctcaccggat gaaaaacgca ttcaggcgat cctcgatcag
    16081 ttaacagatg aaagccgtat cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc
    16141 gcacgttctg gcgaaagcgt cgaagtgcgc gatcggctgg cgctcgacgg taaaaaagca
    16201 ggcggctatt ttaaccagca gattgtcgag ccaattgcgg gtaaaaacgg accgctcggc
    16261 tatctgcgcc tgacactcga cacccatacg ctcgccaccg aagcccaaca ggtggataac
    16321 accactaaca ttttacgcct gatgttgctg ctctcactgg caatcggcgt agtgctgacc
    16381 cgcacgctgc tacagggtaa acgcacccgc tggcagcaat cgcccttcct gttaaccgcc
    16441 agcaaaccgg tgccggaaga ggaagaaagc gagaaaaaag agtgacccat tactacaaga
    16501 aaggaaatcg ttatgtccac attacgcctg ctcatctctg actcttacga cccgtggttt
    16561 aacctggcgg tggaagagtg tatttttcgc caaatgcccg ccacgcagcg cgttctgttt
    16621 ctctggcgca atgccgacac ggtagtaatt ggtcgcgcgc agaacccgtg gaaagagtgt
    16681 aatacccggc ggatggaaga agataacgtc cgcctggcgc ggcgcagtgg cggtggcggc
    16741 gcggtgttcc acgatctcgg caatacctgc tttaccttta tggctggcaa gccggagtac
    16801 gataaaacta tctccacgtc gattgtgctc aatgcgctga acgcgctcgg cgtcagcgcc
    16861 gaagcgtccg gacgtaacga tctggtggtg aaaaccgtcg aaggcgaccg caaagtctca
    16921 ggctcggcct atcgcgaaac caaagatcgc ggcttccacc acggcacctt gctactcaat
    16981 gccgacctca gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa
    17041 ggcattactt cagtgcgttc ccgcgtgacc aacctcaccg agctgctgcc ggggctcacc
    17101 atgagcaggt ttgcgaggcc ataaccgagg cctttttcgc ccattatggc gagcacgtgg
    17161 aagcggaaat catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaacctttg
    17221 cccgtcagag tagctgagag tggaacttcg gtcaggctcc ggcattctcg catctgctgg
    17281 atgaacgctt tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca
    17341 cccgcgccca ggtatttacc gacagcctca accccgcgcc gctggaagcc ctcgccgggc
    17401 gactgcaagg ctgcctgtac cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg
    17461 ttgacttccc ggaccaggaa aaagagctac gggagttgtc gacgtggata gcgggggcgg
    17521 taaggtaact cgctgattaa aatatccaag gaaggaaata tgatcgatct ggaaaaccag
    17581 gaacgggaga ttatcaattt aatgttcagc cagcgtattt cctggctagc cgccgtgcgt
    17641 atcaggcata aattatcgct tgctgaagta agtaaaatgt taggtatctc gattaattct
    17701 ctaaaacaga tcgaaaagac ggaacggctt agctcaaaca tcaaaagcaa aat
//
LOCUS       DAZXUK010000062        17716 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZXUK010000062 DAZXUK010000000
VERSION     DAZXUK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17716
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.062"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(334..1707)
                     /gene="eutA"
                     /locus_tag="ACH3L8_001678"
     CDS             complement(334..1707)
                     /gene="eutA"
                     /locus_tag="ACH3L8_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFT4084834.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(1682..2161)
                     /gene="eutP"
                     /locus_tag="ACH3L8_001679"
     CDS             complement(1682..2161)
                     /gene="eutP"
                     /locus_tag="ACH3L8_001679"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFT4084835.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(2174..2509)
                     /gene="eutS"
                     /locus_tag="ACH3L8_001680"
     CDS             complement(2174..2509)
                     /gene="eutS"
                     /locus_tag="ACH3L8_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFT4084836.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(2802..5081)
                     /gene="maeB"
                     /locus_tag="ACH3L8_001681"
     CDS             complement(2802..5081)
                     /gene="maeB"
                     /locus_tag="ACH3L8_001681"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFT4084837.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            5370..6320
                     /gene="tal"
                     /locus_tag="ACH3L8_001682"
     CDS             5370..6320
                     /gene="tal"
                     /locus_tag="ACH3L8_001682"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFT4084838.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            6340..8343
                     /gene="tkt"
                     /locus_tag="ACH3L8_001683"
     CDS             6340..8343
                     /gene="tkt"
                     /locus_tag="ACH3L8_001683"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFT4084839.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(8446..9489)
                     /locus_tag="ACH3L8_001684"
     CDS             complement(8446..9489)
                     /locus_tag="ACH3L8_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFT4084840.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(9615..10190)
                     /gene="nudK"
                     /locus_tag="ACH3L8_001685"
     CDS             complement(9615..10190)
                     /gene="nudK"
                     /locus_tag="ACH3L8_001685"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFT4084841.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(10258..12237)
                     /gene="aegA"
                     /locus_tag="ACH3L8_001686"
     CDS             complement(10258..12237)
                     /gene="aegA"
                     /locus_tag="ACH3L8_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFT4084842.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            12443..14160
                     /gene="narQ"
                     /locus_tag="ACH3L8_001687"
                     /pseudo
     CDS             12443..14160
                     /gene="narQ"
                     /locus_tag="ACH3L8_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            14362..17475
                     /gene="acrD"
                     /locus_tag="ACH3L8_001688"
     CDS             14362..17475
                     /gene="acrD"
                     /locus_tag="ACH3L8_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFT4084843.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(17574..17633)
                     /gene="ypfM"
                     /locus_tag="ACH3L8_001689"
     CDS             complement(17574..17633)
                     /gene="ypfM"
                     /locus_tag="ACH3L8_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFT4084844.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         4159 a         4729 c         4580 g         4248 t
ORIGIN      
        1 tgtaacttat tattttctaa gcgaaaattt attgtgcggt cagactaaca aacttgcgta
       61 ccacccgaag aacatgcgaa gatttaagta ttctttcttt tttggttttt atttttcatt
      121 ttctcttttc ttctctttcc ggttctgttc tggttttggt tttttccttt ttatttttat
      181 tctttcttca gggttatgct aaaacaccaa aacatttttc accacaaaag catccaatca
      241 tcttgccaga ccaatcacct taaccggata cactgccgga tataatccca ctaacaccca
      301 gacaccaagc accatacctg ataactaccg tattcaggaa ggaaatgcga gtgatttcac
      361 cgtcaccggc acaaccgatc cgccaaaaag aggcgtacca atgtcgatat agtcccccgc
      421 gcggacaatc acttcgtcaa tgactgccaa cgggagttgt tgtagctgtg ggcgcaacaa
      481 catgcccaga gctttaccaa agtcctgccc ggccaccacc agcaggggat gcggattcgg
      541 aaaacgcgcg acgaaatcga ccagcgcgtt gatgaccgtc agaaccgcgg cgtaacgcac
      601 aggcagcgag gcgggaagcg ccagcacgta cacgtcagtt ttgggatcaa gatccagctg
      661 aagcagcgcc tgttgccagg cactcaccag atccgtttca tcaatcggga tcgccaccgg
      721 caaattgcgc agcggcagtt gtacgccctc cagccagatt gtgctgccag agagcgaaag
      781 ggtatgtgca cccgcgccaa tcaccgtgga gcgtacggtt tgcgccggaa actgcacatt
      841 catctcacgc aggcgcggat ggtcatgcag cgccgttgcc agaagtgggc caatatcggc
      901 aaaacagaac gggttggcgg gctggtggcg ataacattcg cccacgcctc cagaaagcgt
      961 aatgatttcg ggcgtaacac ctgcgggcag caaaccggtt tgcatcaatg cctgcgcgag
     1021 cggcgaaagc gttccgtcaa tcacttcgac aatcagcgcc gccatccgcc gggtaacctg
     1081 caccagctgc gcgccggtca gcgaacgggc gtcagtgcct gcaccgaagc actcatccac
     1141 aatcatctgc cccggtttat gagcgtaaac cacgcgcccc tggctgtcgg tttccagcag
     1201 gcgaccaccg acgttgagac aggcagtgcc gctgattttt ccggcatcga acagggcgta
     1261 gttcgcggtg ccaccgccga tatcgatatt cagtacccga cacagccgtt gttcagaaag
     1321 ggtttgcgcc ccggctccgt gaccggcgat cacggattcg aggtgcggcc cggcgctggc
     1381 gacgacaaaa tcgcccagcg actgtttttc cttcatcact ttttccatca gctgtgcgac
     1441 caggctttcg gtaaactggc cttccggcag ctgggcaatg atggtttcgc ggatgcgctg
     1501 gctttcggtt ttgtcggctg gcacgctggc gggcacggac gccgttaccg ggatcgattc
     1561 atcacattcg gtgatggtaa aacccagtag atccgccact tcacgggctt ccggggtaat
     1621 aatgctggcg cgcagaacca cggacattgc ctgttcgccg cgcgcgtgtg cttcacgaat
     1681 atcattcgct gtgatgagtt ttttcacctg cttcctcctg tttggtgaga gatgccagat
     1741 aatccaccag ttgctgcacg ctttgcgggt cgtggctgtt tagctcgaaa atcggctctt
     1801 cgaaacccgt ctcaagcaat aactttcgcg ttgcggcgac gtcagcatct ggcatgtccg
     1861 ttttactgat gacggcgatt tgccgtttgc tgacgccaat atccagcaac ccggcaggca
     1921 accgactttc cgggtcattt gcgccatgga catagatcag catgtctaca tcctgtagcg
     1981 tggtaattaa ggcgtgatac cagcggggat ggctaaaata ttcacccggc gtatcaatat
     2041 cgcccttatc attaaattcc acggccagtg tttttctggc gagggtataa ttcccctgca
     2101 acgcattaaa taacgttgtt tttcctgccc cgacgctacc cacaaacgca attcgtttca
     2161 tgaatgcctc gtattaactt ttggtcattt cacaaagcgt ataatttaat aagcgaccta
     2221 aaccgctgac ggtttgtgat aacgcttctt ctactgcgcc caccgagcca taaatcacca
     2281 atgcaccgct gaagcggtcg agaaaaccga tatgcacatc agcggctttc agcgctaaat
     2341 caccggcgat catcgccgtt tcgccgggag ttagcgtcat aatgccaatt gcgcccgcat
     2401 ccggaacgcc gattttcttc gccagttcct caccgggatg cgcgatgaga tgcgccagtg
     2461 tgacctgttt gccgggcaca aattcctgaa tgatgcgttc tttatccatg agtcgccgcc
     2521 tgtgaaaaac tttcgtgtat cgtgacaatt tttcgtcaga gtaaataaca aaattcggca
     2581 agcgcgttta aaaggtgagg tagatcaata agaatggaga gatatttact gttatgaata
     2641 aactttcata tgcgacgcat gaatataaat aaaataatat cgagatattt tatatactga
     2701 tttttttatt attcgcaaac tcagtaataa gctttgctaa tatatttcag gaaaaccgct
     2761 taccctgaat attcagggta agcgtgagag ttaagaatga attacagcgg ttgggtttgc
     2821 gcttcaacca cggccagcgc caccatattg acgatacgac gcaccgatgc gatcggcgtt
     2881 aacacgtgaa ccggtttcgc cacgcccatc agcaccggac cgacagtcac accttccgag
     2941 ctggaaacgc gcagtaagtt gtaactaatg cgggcagctt ccatgttcgg catcaccaga
     3001 atattggcag aacctttcaa agggctgtcc ggcatacggt cgttgcgaat cgcttccacc
     3061 agcgctgcat cgccgtgcat ttcaccatca atcatcagat ctggcgcacg atccctgacc
     3121 agttccagcg cctgacgcat tttgctcgac gacgggcagt cagaagaacc aaagttggag
     3181 tgcgacaaca gagcaacgcg cggctcaata ccaaaacgac ggacagtttc tgccgccatc
     3241 aaggtgatct ccgccagctc ttctgcgtct ggttcgtcat tgacgtaggt atcggcaata
     3301 aaggtgttac cactcggcag cagcagcgca ttcattgcac ctgcggtgtg aacgccatcg
     3361 cgataaccaa agacattttt caccacacta aaatgttcat gataatcgcc caccgtaccg
     3421 cagatcattg catcggcttc cccacgctga accatgatcg cgccgatcac tgtcgggtta
     3481 ctgatcagcg cacgctgcgc ctgttcctga gtgacaccgc gacgcttcat gatctggaag
     3541 tattcggtcc agtactcttt aaagcgcgga tcggattcgt tattgacgat ttcaaaatca
     3601 acgcccgctt tgatctgcaa gcccagtttc tgaatgcgca tttcgatcac gttcggacga
     3661 ccgataagga tcggtttcgc cagtcccagc gttaccagtt cctgagtcgc atgcagaacg
     3721 cgcgcctctt ccccttccgg cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa
     3781 atcggcttca taaacaggtt ggttttgtaa acgaactcag tcagcttgtc gatgtagacg
     3841 tcgaaatcag caatcggacg cgtcgccacg cccgactcca tcgccgcttt tgcgaccgca
     3901 ggagcgatct taacgatcaa gcgcggatca aacggttttg gaatgatgta ttccggacca
     3961 aagctcagat cctgaacgcc atacgctgaa gccaccactt cactctgttc tgcatgggcc
     4021 agttctgcaa tcgcgcgtac cgccgccagt ttcatctctt cgttgatggc ggttgcgcca
     4081 acgtccagcg cgccacggaa gatgaacggg aagcaaagaa cgttgttcac ctggttcggg
     4141 tagtcggaac ggccggtaca gatgatggca tccggacgca cttctttcgc cagcggcggc
     4201 agaatttccg gttccgggtt cgccagcgcc aggatcattg gtgcccgagc cattttcttc
     4261 accatttcct gggtcagcac tttcgggccg gaacagccca ggaaaatatc cgcaccttca
     4321 atcacatcat cgagggtacg tttgccgtca tccaccaccg catatgcggc tttggtttcc
     4381 gccatatttg gctcacggcc ctgatagata acgccttttg aatcgcaaac cacgatgtta
     4441 tgtttttgca gccccagcgc taccagcagg ttcatacagg cgattgctgc ggcacccgcg
     4501 ccggaaacca ccatccgcac gtcggagatg tttttctcca ccacgcgcaa gccgttgagg
     4561 atggcggcag tgctgatgat tgccgtgccg tgctgatcgt cgtggaatac cggaatattc
     4621 atccgctcgc gcagtttctg ttcaatatag aaacattctg gcgctttaat gtcttcgagg
     4681 ttgatgccgc cgaaggtcgg ttcgagcgcg gcgacgactt caataaattt gtccgggtcg
     4741 agttcatcaa cttcaatgtc aaatacatca atcccagcga atttcttaaa tagaacgccc
     4801 ttgccttcca tcaccggttt gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc
     4861 gtaccgttag agatcaccgc aaccaggtta ccgcgcgcgg tatatttgta ggcttttagc
     4921 gggtcttttt cgatttcaag acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga
     4981 tcgcgctgcg ttgccagagg cttggttgga gaaacctgga ttttccctgg aactggaaat
     5041 tcatggaaat caagtgcact ttgttttaac tggtcatcca tttggttgtt cctttcacgt
     5101 aacgttcaca aataaagtgt gtgggcaaca gcccctgccc acaacgtggc gcacattatt
     5161 accctgccgg agtctacaga ctttgagcaa gtccaaactc tcaccattaa tataatgttt
     5221 tggtaataat cctataacac tgatgttacc tgcttaatcc agcaatacca tgcctgtctg
     5281 ctatgctttt ttgatgcgtt tagcgaaatt tctcagaagt gtgaattaac gcactcatct
     5341 aacactttac ttttcaagga gtatttccta tgaacgagtt agacggcatc aaacagttca
     5401 ccactgtcgt ggcagacagc tgcgatattg agtccattcg ccattatcat ccccaggatg
     5461 ccaccactaa tccttcgctg ttactcaagg ctgccggatt atcacaatat gagcatttaa
     5521 tagacgatgc tatcgcctgg ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg
     5581 cagcgtgtga caaactggcg gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtt
     5641 gcgtgtcaac agaagttgat gcacgcctct cttttgataa agaaaagagt attgagaagg
     5701 cgcgccatct ggtggacttg tatcagcaac aaggcgttga gaaatcacgc attttgatca
     5761 aactggcttc gacctgggaa ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta
     5821 actgcaacct gacgctgctg ttttctttta cccaggcgcg ggcctgtgcg gaagcaggcg
     5881 tttttctgat ttcgccgttt gtagggcgta tttatgactg gtatcaggca cgcaagccga
     5941 tggacccgta tgtggtggaa gaagatccgg gcgttaaatc ggtgcgcaat atctacgact
     6001 actataagca acaccactat gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac
     6061 aaatcctcgc cttaaccggc tgcgatcgac tgactatcgc accgaatttg ctgaaggagc
     6121 tgcaggaaaa agtttctcca gtggtacgta aattaatccc gccttctcag acgttcccac
     6181 gcccagcacc catgagcgaa gcggagttcc gttgggagca caatcaggat gcgatggcgg
     6241 tagaaaaact gtctgaaggc attcgtctgt tcgctgttga tcaacgcaaa ctggaagatc
     6301 ttcttgccgc caaactataa accagccacg gagtgttata tgtcccgaaa agaccttgcc
     6361 aatgcgattc gcgcactcag tatggatgct gtacaaaaag ccaattctgg tcatcccggc
     6421 gcgccgatgg gcatggctga tattgccgaa gtgctgtgga acgattttct taaacataat
     6481 cctaccgacc caacctggta tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg
     6541 atgctgctct acagtttgct acatctgacc ggttacgacc tgccgctgga agaactgaag
     6601 aacttccgtc agttgcattc gaaaacccca ggccacccgg agattggcta tacgccaggc
     6661 gttgaaacca ccaccggccc gcttggacaa ggtttggcga acgccgtcgg gctggcgata
     6721 gcagagcgta cactggcggc gcagtttaac cagccagacc atgagatcgt cgatcacttc
     6781 acctatgtgt ttatgggcga cggctgcctg atggaaggta tttcccacga agtctgttcg
     6841 ctggcaggca cgctgggact aggcaagctg attggttttt acgatcacaa cggtatttcc
     6901 atcgacggtg aaacagaagg ctggtttacc gacgatacgg caaaacgttt tgaagcctat
     6961 cactggcatg tgatccatga aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc
     7021 cttgaagcgc aaagcgtgaa agataagccg tcgctgatta tctgccgtac ggtgattggc
     7081 tttggttcgc cgaataaagc aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa
     7141 gaagtggcgc tggcacggca aaaactgggc tggcaccatc cgccatttga gatccctaaa
     7201 gagatttatc acgcctggga tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat
     7261 gagaagtttg ccgcctataa aaaggctcat ccgcaactgg cagaagagtt tccccgacgg
     7321 atgagcggtg gtttaccgaa ggactgggag aaaacgactc agaaatatat caatgagtta
     7381 caggcaaatc cggcgaaaat cgctacccgt aaggcttcgc aaaatacgct taacgcttac
     7441 gggccgatgc tgcctgagtt gctcggcggt tcggcggatc tggctcccag caacctgacc
     7501 atctggaaag gttctgtttc gctgaaggaa gatccggcgg gcaactacat tcactacggg
     7561 gtgcgtgaat ttggcatgac cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg
     7621 ccgtataccg cgacgttcct gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg
     7681 gcactgatga aagcgcggca gattatggtt tatacccacg actcaattgg cctgggcgaa
     7741 gatggtccga cgcaccaggc tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc
     7801 agcacctggc gaccgtgcga tcagatggaa gcggcggtgg gctggaagct ggcggttgag
     7861 cgccacaacg gaccgacggc actgatcctc tcaaggcaga atctggccca ggtggaacgt
     7921 acgccggatc aggttaaaga gattgctcgt ggcggttatg tgctgaaaga cagcggcggt
     7981 aagccagata ttattctgat tgccaccggt tcagagatgg aaattaccct gcaagcggca
     8041 gagaaattag caggagaagg tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt
     8101 ttcgacgccc aggatgagga atatcgggag tcggtgttgc cttctaacgt tgcggctcgc
     8161 gtggcggtgg aagcaggtat tgccgattac tggtacaagt atgttggtct gaaaggggca
     8221 attgtcggga tgacgggtta cggggaatct gctccggcgg ataagctgtt cccgttcttt
     8281 ggctttaccg ccgagaatat tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc
     8341 tgatggtgat cgccggatgc tgtatgttcc cctcacccta accctctccc cagaagggcg
     8401 aggggacggt ccgtgcgctt tttagggccg catctgcgat tttgtttaac gggtgatcca
     8461 caacgtgggc ctggcgtctg gcccatgcca gttatcgcag gtaggttctg cggcgtaacg
     8521 caccaggcga aaacgctgac cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa
     8581 tccgcgccct ttcgctaagg tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat
     8641 cagttccagt tcattcgtct cctggccgtt gttgaacggc aaacgcaacc gaaccgggcg
     8701 cgaggctagc ggctttttac gcgacacaat ccatgccaaa tcaatggtgt tataggcccc
     8761 tgcctcacag ctaatcatca tcagtgcttt atcatcggtc agcgcagtga cattcacctc
     8821 acgacgtaac ggatcaagcg agcagcgcag accattcatc cgccagtttc cataatccag
     8881 caaatcgtta cgttcttcga gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc
     8941 tttcagcgca ggcgcgggcg gtacgctgag aggcggttcg tcccctttct tgatccacgc
     9001 ggtttcactg ccaacgcgct tttgctgagc atcaataaac aacaacgccg cttttaagcc
     9061 gctcagagaa atggtctgat tgccgtcgcg taaggtgatc gccttccctt cctgaattat
     9121 ctgcaaaaac gcggtgatgg ttgccgtatc atcggttact aataaccatg gtgaaatccg
     9181 ccacttgtca ccacttaatg ccaacggctc accatctaat agcagccgtg gcgctatctc
     9241 cccttcagac gcatccggcg acttcaaccc accgcgctca atacgtaaaa cggcatcggt
     9301 atgcgcccca gcgctgcggc tcagggtcat caccagtcca ttatgatcgc ccgtattgcg
     9361 cgccacacaa aaattttgat tattacaggt gacctgccag tcggaaaacg cccgttgcgc
     9421 tggtgccgcc cacaccaaag acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg
     9481 atagcgcatg gacggtacga ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt
     9541 tgcgcaggta gcgcggctca atcggatttg tcggattgag ccaataataa acaacgaaat
     9601 attattgcta tttttcagtc cattaaatgt gacatttgca aatagttaag caataacacc
     9661 gtcttaccgt cacgtatctc gccggttttg atcatctcca gcgcctggct gaacggcagc
     9721 tcgagcactt caatatcttc atcttcaaca ccgccaccgg cgttagcgcg ctgattgtca
     9781 ctgtattcgg cgataaaaaa gtggattagc tcagtcacac cgccaggcga catatacagt
     9841 tcaaataatt tgcgcacttc gcccacttca tagcccgtct cttcaatcgc ttctttacga
     9901 atgcacactt ccggttcgtc gttatccagc agcccggcgc aggtttcaat cagctgcccg
     9961 ctttcattgc cattaaccca ggtagcgaca cggaactgac gaatcagaac cacgctcttt
    10021 ttcttcgcgt tgtacaggag gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta
    10081 tggcggataa cttcgccatc tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa
    10141 tagttatcgg agagaatttt gtctttaatg agggtgattt gttgcgtcat accgactcca
    10201 cagcgcgaaa tgaacaatta tattacgctg tgaagtcggg tttgtctgcg caggctatca
    10261 gtgagatttg actgatttta cccccagcca gtcaataatc ccctgtgccg catgacgtcc
    10321 ttctgccatt gcggtaacca ccagatccgc accacgcacg gcgtcgccac cggcgaagat
    10381 tttcggattg gtggtctggt aacggtactg gctttccaca tccgcgatga tgcggcccca
    10441 tttgtctacc gttacaccgt gcgactccag ccacggcatc ccgtgtggat tgaagccaaa
    10501 cgccataatc accgcatcgg ctggcatgac aaattcactg ccctcaatcg gtacaggtcg
    10561 ccgacgcccc tgggcatccg gctcgccaag acgcgtgcgc aggaaacgaa ttccgcaaac
    10621 gtgaccttgc tcattcagct caagcgccac cggctggacg ttaaattcga agttggcccc
    10681 ctcttcgcgg gcgttcttca cctctttctt cgagcctggc atgttagctt catcacgacg
    10741 ataagcgcag gtgacgttac tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc
    10801 ggtgtcgccg ccgcccagta ccacgacgtt aagtccggcg gtattgataa acggctcttc
    10861 cggtagctct tcgagcccca tcacctgttt agtgttggca atgaggaacg gcagcgcgtc
    10921 ataaacgccc ggcgcatctt cattgggtaa acccgctttc atggaacggt aagtgcctac
    10981 gccaacgaag accgcgtcgt attgttccaa aagcaaatcc aaagagacat ctttacccac
    11041 ttcacaattg agttcgaagt gaatccccat cgcgctgaag atttcccgac ggcgtgccag
    11101 cagggattta tccagtttga aagaaggaat gccgaaagtg agcaagccac cgatttctgg
    11161 atggcgatcg tacaccgtca ccgccacgcc attgcgggtc agaacatccg cacaggccag
    11221 ccctgccgga cctgccccga taatcgccac ccgcttgtcc actttggtga catgacttaa
    11281 gtcaggacgc caacctttcg ccaacgcctg atctgaaata tagcgttcaa tgttgccgat
    11341 agttaccgcg ccgtgctcat cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg
    11401 acaaacgcgt ccggtaattt ccggcagggt gttggtctgg tgagaaagct cgacggcggc
    11461 gtcgatgttt ccggctttca ccagttcaat ccactgcgga atatggttat gcagcgggca
    11521 ggtccattca caaacgctgt gctcgccgaa cttaaggcag cgcgaggctt cccgttgtgc
    11581 ctggtcggcg cgaaatggca gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc
    11641 cagtttatcc ggctcgccac gcgcgggcgt tgcctgcatt tgttcgactt tactcattac
    11701 cggcatttct tgtgcagcgg tactggcatg ccacggttga tgctcctgac gtgcggtgcg
    11761 caagcggcgg gatttcgcca tgccggagag tgcgacgtca gtgaccagtt gcagcgcgtc
    11821 cgccgggcaa ttctcgacac aggcaggacc gttttcgcgc cctgcacaaa ggtcgcattt
    11881 atgcgccgtg gcttttactt ttcctgccgc gacgggtgtc aggacgattt gcatcgtacc
    11941 aaacggacag gccaccacgc aggatttaca gccaatacac ttttgctgat tgacctgaat
    12001 gctgtcatca acgtggctga ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc
    12061 acaatggtga caggtcactg cactacgttg ctgctgatgt ttgataaccg taattcgggg
    12121 atgaaaatgg tgttggctca ggacatgttg ctcatcattg tgagccatga cacaggcgat
    12181 ttcacaagca tgacaaccca gacattgctg actgttggcc ataataaaac gattcataac
    12241 gaccttcttt tttggttgta aaaaccttat tctttatatg agtgttgtta ttacccgact
    12301 tacaggggaa ccggcaatgt tcatgtgccc agaataagta actatttcgc tataaactgt
    12361 ggcagatcaa ataatcccat cactgactaa attgcgtttc agcgaactgg aacattaatg
    12421 attttttgtg gagaagacgc gtgtgattgt taaacgaccc gtctcggcca gtctggcccg
    12481 ggcctttttt tacattgtgc tgctgtcgat tctttccacg ggtatcgctc tgctaactct
    12541 ggcgagcagt ttgcgcgacg ctgaggctat caatattgcc ggatcgctgc gtatgcagag
    12601 ttaccgcctg ggctacgact tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt
    12661 gtttcagcag gcactgcatt caccggtatt aaccaacctc aacgtctggt atgtgccaga
    12721 agcagtaaaa actcgctatg cgcatctgaa tgccaactgg ctggagatga ataatcggct
    12781 cagcaagggc gatttgccgt ggtatcaggc caatattaat aattatgtta atcagataga
    12841 cctgttcgtg ctggctttac agcactacgc tgaacgcaaa atgctgctgg tggtggcgat
    12901 ttccctggct ggcggtatcg gtattttcac gctggtcttt tttactttgc gccgcatacg
    12961 ccatcaggtg gttgccccgc tgaatcagct ggttaccgcc agtcagcgta ttgaacacgg
    13021 gcagttcgac tcgccgccgc tggataccag tctgccgaat gagcttggtc tgcttgcaaa
    13081 aacctttaac caaatgtcga gcgagctgca taaattgtac ctttcgctgg aggcgtcagt
    13141 agaagaaaag acccgcgatc tccacgagac caagcgtcgt ctggaggtgt tgtatcagtg
    13201 cagtgttccc aggcgctgaa caccagccag attgatgtgc attgtttccg ccatattttg
    13261 cagattgttc gcgacaatga agcagctgaa tatctggagc tgaacgttgg ggataactgg
    13321 cggattagcg aagggaagcc aaaccctgag ctgccaatgc agattttacc ggtgacaatg
    13381 caagagacgg tttacggcga actgcactgg caaaatagtc acgtttcatc atcagaaccg
    13441 ctgcttaaca gtgtttcatc gatgctggga cgcggtttgt actttaatca ggcgcagaag
    13501 cattttcagc agttattgtt gatggaagaa cgtgcgacca tcgcccgcga attgcacgac
    13561 tcgctggctc aggtgctttc ttacttacgt atccagttga cgttactgaa gcgttcgata
    13621 ccggaagata acgccaccgc acaaagtatc atggccgatt tttcccaggc gttgaatgat
    13681 gcttatcggc agttacgcga gctgttgact actttccgcc tgacgctgca gcaggcggat
    13741 ctcccctccg cgttgaggga aatgctggat acgttacaaa atcaaaccag cgctaaactg
    13801 accctcgact gccgtctgcc aaccctggca ctggatgcgc aaatgcaggt gcatttgttg
    13861 caaattattc gcgaagcggt gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc
    13921 gtcagttgcg tcaccgcgcc ggacggcaat cacacggttt atatccgtga taacgggatt
    13981 ggtatcggtg aaccgaaaga acccgaaggt cattatggac tgaatatcat gcgcgaacgc
    14041 gcggaacggc tgggcgggac gctgactttt tcacaacctt ccggcggcgg cacgttagtg
    14101 agtattagct ttcgctccgc agagggtgag gggaagggga acacgccggc tgaggtgtaa
    14161 ataattaccc tcacaccggc cctctccctt taaaggcgag agggaaaaac gtgcccatat
    14221 tgacctgtaa gcgagtgtgt cagcaatctc atgataattt acattaactc ctttttttct
    14281 ccacgattgg ctcgtacctt gccgctacag tgaagcaagt caagcctaca acaatacgca
    14341 gaaacacgag gtcctctttt aatggcgaat ttctttattg atcgccccat ttttgcctgg
    14401 gtgctgacaa tcctgttgtg tctgacaggt accctggcga ttttttcatt gcccgttgaa
    14461 caataccccg atctcgcgcc accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg
    14521 gcccagacgc tggaaaacac cgtgacccag gttatcgagc aaaatatgac cggcctcgat
    14581 aatctgatgt atatgtcatc tcagagcagt ggcaccggtc aggcatctgt cactttaagt
    14641 tttaaagcag gcaccgatcc ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca
    14701 gccatgcgaa agttaccgca ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc
    14761 gataccaaca ttctgaccat tgccttcgtc tctaccgatg gctcgatgga taaacaggat
    14821 attgctgatt atgttgccag taatattcag gacccgttaa gccgcgtgaa tggcgtcggg
    14881 gatatcgatg cctatggttc gcaatattcc atgcgtatct ggctggaccc ggcgaaactc
    14941 aacagtttcc agatgacggc taaagatgtc actgatgcca ttgagtcaca gaacgcgcag
    15001 attgcggttg ggcaacttgg tggtacacct tccgtcgata agcaggcgct caacgccacc
    15061 attaacgccc agtcactgct gcaaacacca gaacagttcc gcgatatcac cttgcgggtg
    15121 aatcaggacg gctcagaggt aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag
    15181 aaatacgatt atcttagccg cttcaatggt aagccagcct ccgggttggg ggtaaaactg
    15241 gcctccagcg caaacgaaat ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg
    15301 gcgcagtatt tcccgcacgg actggaatac aaggtggcgt atgaaaccac ctcgtttgtt
    15361 aaagcctcca ttgaagacgt ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc
    15421 gttatgtatc tgttcctgca aaacttccgc gccacgctga tacccactat cgccgtgccg
    15481 gtggtgttga tgggaacctt ctccgtactt tacgccttcg gttacagcgt caacacctta
    15541 accatgttcg cgatggtgct ggcgatcggt ctgctggtgg atgacgccat cgtggtggtg
    15601 gaaaacgtcg aacgtattat gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa
    15661 tcgatggggc agatccaggg ggcactggtc gggattgcga tggtgctttc ggcggtattt
    15721 gtaccaatgg ccttcttcgg cggcaccacc ggtgccatct atcgccagtt ctctattacc
    15781 attgttgcgg cgatggtgct gtcagtactg gtagcgatgc tcctcactcc ggctctgtgt
    15841 gccacgctac ttaagccact gaaaaaaggt gagcatcacg ggcaaaaagg tttttttgcc
    15901 tggtttaacc agatgtttaa ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt
    15961 ctccaccgta gcctgcgctg gattgtgatt tatgtcctgc tgcttggcgg catggtgttc
    16021 ctgttcctgc gtttgccgac gtcgttctta ccgctggaag accggggcat gtttactacc
    16081 tcggtacaat tgcccagcgg ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc
    16141 gagaaatatt acttcaccca tgaaaaagac aacatcatgt cggtgtttgc caccgttggt
    16201 tctggccctg ggggtaacgg gcaaaacgtg gcgagaatgt ttatccgcct gaaagactgg
    16261 agcgaacgcg acagtaagac cggcacctcg tttgccatta tcgagcgtgc aacgaaggcg
    16321 tttaaccaaa ttaaagaagc tcgcgttatc gccagcagcc caccagcaat tagcggtctt
    16381 ggtagttctg caggttttga tatggagttg caggaccacg ctggagcggg tcacgatgcg
    16441 ctgatggcag cacgtaatca gttgctggcg ctggcggcgg aaaacccgga gctaacccgt
    16501 gtgcgccata acggcctcga tgacagtccg cagttgcaga ttgatatcga ccagcgtaaa
    16561 gctcaggcgc tgggcgttga tatcgacgat attaacgaca cactgcaaac cgcctggggt
    16621 tcgagctatg tgaatgactt tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca
    16681 gctgcgccgt atcgcatgct gccagatgac atcaatctct ggtatgtccg aaataaagat
    16741 ggcggcatgg tgcccttctc tgctttcgcg acctcacgct gggaaacagg ctcgccgcgt
    16801 ctggaacgct ataacggtta ttctgcggtt gagattgttg gggaagccgc accgggggtc
    16861 agtaccggta cggcgatgga tattatggaa tcgttagtga agcagctgcc aaacggcttt
    16921 ggtctggagt ggacggcgat gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg
    16981 ctgtacgcca tttccttgct ggtggtattc ctgtgtctgg cggcattgta tgaaagctgg
    17041 tcggtaccgt tctcggtaat gctggtcgtg ccgctggggg taatcggcgc gctgctggca
    17101 acctggatgc gcgggctgga aaacgacgtt tacttccagg tgggcctgtt aacggtcatt
    17161 ggtttatcgg cgaaaaacgc catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa
    17221 ggccatgacc tgtttgaagc gacgctccac gcctgccgtc agcgtttacg cccgattctg
    17281 atgacctcgc tggcatttat cttcggcgta ttgccaatgg caaccagcac gggtgccggt
    17341 tccggtggtc agcatgcggt gggtactggc gtaatgggcg ggatgatttc agctactatt
    17401 ctggctattt acttcgtgcc gctgttcttt gtgctggtgc gccgccgctt cccgctgaag
    17461 ccgcgcccgg aataagcaat aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt
    17521 ccgattaaga acctgctcag cgggttcttg ctgtttatac tttgtctcag gaattactta
    17581 cgaagcataa cttcgataaa gtctttccag ttccccagtt cacgttcaat cataacaacc
    17641 tctcttataa ttatgggtat tctacggaaa caatataccg tggtgaagct aatttactcg
    17701 attgctgcga tgacta
//
LOCUS       DAZXUK010000063        17713 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZXUK010000063 DAZXUK010000000
VERSION     DAZXUK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17713
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.063"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            108..791
                     /locus_tag="ACH3L8_001690"
     CDS             108..791
                     /locus_tag="ACH3L8_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFT4084845.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            835..5497
                     /locus_tag="ACH3L8_001691"
                     /pseudo
     CDS             835..5497
                     /locus_tag="ACH3L8_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            5865..7520
                     /gene="lldP"
                     /locus_tag="ACH3L8_001692"
     CDS             5865..7520
                     /gene="lldP"
                     /locus_tag="ACH3L8_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFT4084846.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7520..8296
                     /gene="lldR"
                     /locus_tag="ACH3L8_001693"
     CDS             7520..8296
                     /gene="lldR"
                     /locus_tag="ACH3L8_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFT4084847.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8293..9483
                     /gene="lldD"
                     /locus_tag="ACH3L8_001694"
     CDS             8293..9483
                     /gene="lldD"
                     /locus_tag="ACH3L8_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFT4084848.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9679..10152
                     /gene="trmL"
                     /locus_tag="ACH3L8_001695"
     CDS             9679..10152
                     /gene="trmL"
                     /locus_tag="ACH3L8_001695"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFT4084849.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10205..11026)
                     /gene="cysE"
                     /locus_tag="ACH3L8_001696"
     CDS             complement(10205..11026)
                     /gene="cysE"
                     /locus_tag="ACH3L8_001696"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFT4084850.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11106..12125)
                     /gene="gpsA"
                     /locus_tag="ACH3L8_001697"
     CDS             complement(11106..12125)
                     /gene="gpsA"
                     /locus_tag="ACH3L8_001697"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFT4084851.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12125..12592)
                     /gene="secB"
                     /locus_tag="ACH3L8_001698"
     CDS             complement(12125..12592)
                     /gene="secB"
                     /locus_tag="ACH3L8_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFT4084852.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12655..12906)
                     /gene="grxC"
                     /locus_tag="ACH3L8_001699"
     CDS             complement(12655..12906)
                     /gene="grxC"
                     /locus_tag="ACH3L8_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFT4084853.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13048..13479)
                     /locus_tag="ACH3L8_001700"
     CDS             complement(13048..13479)
                     /locus_tag="ACH3L8_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFT4084854.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13724..15268
                     /gene="gpmM"
                     /locus_tag="ACH3L8_001701"
                     /gene_synonym="pgmI"
     CDS             13724..15268
                     /gene="gpmM"
                     /locus_tag="ACH3L8_001701"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFT4084855.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15302..16561
                     /gene="envC"
                     /locus_tag="ACH3L8_001702"
     CDS             15302..16561
                     /gene="envC"
                     /locus_tag="ACH3L8_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFT4084856.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16565..17524
                     /locus_tag="ACH3L8_001703"
     CDS             16565..17524
                     /locus_tag="ACH3L8_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFT4084857.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17511..17713)
                     /locus_tag="ACH3L8_001704"
                     /pseudo
     CDS             complement(17511..17713)
                     /locus_tag="ACH3L8_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4423 a         4611 c         4641 g         4038 t
ORIGIN      
        1 atcgacacac atcactgtca ttaatgtaaa gcaacatagc tcaccaataa tacaatggac
       61 aactctaccg gcattgagcg ctggtcggag gaaataagga tttttacatg aacttgaaga
      121 agattttttt cagtgctgtc actgtttcag ttctctgcgc attaacggga tgtgactaca
      181 ttgaagaagg caagccagaa agtagtttac tgaaacaaca agaagaacat aataataaaa
      241 ttgtgctact tgaaaaacaa caagcacagc taaagagtca actcgaaact attcaaaaac
      301 aacaaactgg cataataaac agtacaaaga ctttaactca cgtaattaaa tccgttaaag
      361 accaacaaaa tacttttatt tttaccgagt ttaacccagc aaaaacgaag tatttcatcc
      421 tgaacaacgg ttcggtggct ttagcgggtc gagtgttatc cattgacgcc acagagaatg
      481 gtagtgttat tcatatctca ctggtcaact tattaagtac acctatctca aacattggtt
      541 ttaatgcgac atggggtggt gaaaaacctg tcgatgccaa agagtttgcc cggtggcaac
      601 aattactttt caacacatca atgaaatcca cattgaaatt attaccaggt caatggcaag
      661 acattaattt gaccctgaag ggtgtatcgc ccaataacct gggatatctg aagttagcca
      721 tcaacatgga aaatattcag ttcgacaatc ttccctctgc tgaaaatcgg cagaaaagaa
      781 gcaaaaaata aatgcagtta gtacgcttat aacaaggaat agcgagcatc attaatgaac
      841 aaaatattta aagttatctg gaaccctgcg acagggaatt atactgttac cagcgaaacg
      901 gcaaaaagcc gtggcaagaa atctgggcgc agtaagctgt taatttctgc gctggttgcg
      961 ggtggaatgt tgtcgtcgtt tggggcattg gcgaatgccg ggaatgacaa cggtcagggt
     1021 gttgattacg gtagtggatc agctggcgac ggctgggttg ctataggcaa aggggcgaaa
     1081 gcaaatactt ttatgaacac cagtggttcc agtactgctg tgggttatga cgctatagct
     1141 gaaggccaat atagctctgc catcgggtca aaaacccatg cgattggtgg tgcatcaatg
     1201 gcctttgggg ttagtgcaat atcagaaggc gatagaagta tagcactggg tgcctcttcg
     1261 tattcattgg gccaatactc aatggccctc ggccgttatt caaaagcatt gggtaaattg
     1321 tctattgcta tgggggactc ttccaaagcg gaaggagcaa acgccattgc cctggggcgt
     1381 agcagtgtag ctagcggtac tgacagcctc gcatttggca gacaatcact tgccagcgca
     1441 gcgaacgcta ttgcgatagg tgctgagacc gaagccgctg aaaatgcaac tgctattggc
     1501 aataatgcga aggcaaaagg gactaatagc atggcaatgg ggttcggaag ccttgccgat
     1561 aaagtcaata ctatcgcatt aggaaatggc agccaggctc tggcagataa tgcaatcgcc
     1621 ataggccagg gcaacaaagc tgatggcgtg gatgccatcg ctctgggtaa tggtagccag
     1681 tcgagaggct taaacaccat tgccttaggc acagccagta atgcaactgg tgataagagt
     1741 cttgcgcttg gtagtaatag cagtgccaac ggtattaact ctgtcgcgct gggcgcagat
     1801 tccattgcgg atttagacaa taccgtctct gtcggcaata gttcattaaa acgcaagatc
     1861 gttaatgtga aaaatggcgc gatcaagtct gacagttacg atgccattaa tggttcacag
     1921 ctttatgcca ttagcgactc ggtagcaaaa aaggcttgga ggaggggctg cagtagatgt
     1981 tgatgacggt actgttacag caccaaccta caatttaaaa aatggtagca aaaataacgt
     2041 aggggctgcg ctcgctgtac ttgatgaaaa caccctgcaa tgggaccaaa ccaaaggcaa
     2101 atacagcgct gctcatggta ctagtagccc aactgccagc gtaatcaccg atgttgcgga
     2161 tggcacgatt tcagcctcca gtaaggatgc ggttaacggt tcccaactga aagctaccaa
     2221 tgacgatgtc gaagccaaca ccaccaatat caccaacctg acggattccg ttggtgacct
     2281 tcaggctgat gccctgctct ggaacgaaac taaaaaggca ttcagtgcag ctcacggcca
     2341 ggataccacc agcaaaatca ccaacgttaa agatgccgac ctgacggctg acagcactga
     2401 tgctgttaac ggctctcagc tgaaaaccac caacgatgct gtggcgacga ataccaccaa
     2461 tatcgccaat aacacttcca atattgccac taacaccacc aacatctcta acctgactga
     2521 gacggtgact aatcttggtg aggatgcgct gaaatgggat aaggacaatg gtgtattcac
     2581 ggcagctcat ggcaccgaga ccaccagcaa aataaccaac gttaaagatg gcgacctgac
     2641 gactggcagc accgatgccg ttaacggctc tcagctgaaa accaccaacg atgccgtggc
     2701 gacgaatacc accaatatcg ccactaacac caccaacatc tctaatctga ctgagacggt
     2761 gactaatctt ggtgaggatg cgctgaaatg ggataaggac aatggtgtct tcactgcagc
     2821 tcatggcaac aataccgcca gcaaaatcac caatatcctg gacggcacag tcactgcaac
     2881 cagttccgat gccattaacg gtagccagct ttatgactta agcagcaata tcgccaccta
     2941 cttcggcggc aatgcttctg tgaatactga cggtgtgttt accggtccaa cctacaaaat
     3001 cggtgaaaca aattattata acgtcggcga tgcactggct gcgattaact cctcatttag
     3061 cacgtctctc ggcgatgctc tgctttggga tgccaccgca ggtaaattca gtgccaaaca
     3121 cggtactaat ggtgacgcaa gcgtgatcac tgatgtcgca gatggtgaaa tttcagactc
     3181 cagttctgac gcagtaaacg gctcacaact ccacggcgtg agcagttatg ttgttgatgc
     3241 gctggggggt ggtgccgaag tcaatgcaga cggcaccatc actgcgccga cgtacaccat
     3301 tgctaatgct gattacgata atgtcggtga tgccctgaat gctatcgata ccactcctga
     3361 cgacgctctg ctctgggatg cggacgccgg tgaaaatggt gcatttagcg ccgctcacgg
     3421 aaaagataaa actgccagtg taatcactaa cgtcgctaac ggtgcaatct ctgctgccag
     3481 cagcgacgcg attaacggct cacaactcta taccaccaat aagtacatcg ctgatgcgct
     3541 gggtggtgac gcagaagtca acgctgacgg caccatcacc gcaccgactt acaccattgc
     3601 gaacgccgag tacaacaacg tcggtgacgc cctggatgcg cttgatgata acgccctgct
     3661 gtgggataag actgccaatg gcggtgctgg agcctacaat gccagccatg acggtaaagc
     3721 cagcatcatc actaatgtcg ctaatggcag tattagtgag gacagtaccg atgcagtgaa
     3781 cggttctcag ttgaatgcga cgaatatgat gattgagcag aacacccaaa ttatcaatca
     3841 gctcgctggt aacaccgacg caacctatat cgaagaaaat ggcgctggta tcaactacgt
     3901 tcgtactaac gacaacgatt tagcctttaa cgatgcaagc gcctctggtg tcggcgctac
     3961 agctgtaggt tataacgctg tcgcgtctgg tgccagcagc gtagccattg gtcagaacag
     4021 cagcagcacc gttgataccg gtattgcgct gggtagcagc tccgtttcca gccgtgtgat
     4081 tgccaaaggt tctcgtgaca ctagcgtaac ggaaaatggc gtggtgattg gttatgacac
     4141 cactgacggc gaactgctag gtgcattgtc aattggtgat gacggtaaat accgccaaat
     4201 catcaacgta gctgatggtt cagaagccca tgacgccgtt acggttcgcc agttgcagaa
     4261 cgctattgga gcggtcgcca ctacgccaac caagtacttt cacgccaact caacggcaga
     4321 agactcactg gccgttggtg aagactcgct ggcaatgggt gcgaaaactg tcgttaatgg
     4381 taatgcaggt attggtatcg gtttgaacac tctggttctg gctgatgcga tcaacggcat
     4441 tgctatcggc agtaacgcac gcgcaaacca tgcaaacagt atcgcaatgg gtaacggttc
     4501 tcagaccacc cgtggtgcac agactggcta caccgcctac aacatggacg caccgcagaa
     4561 ctctgtaggt gagttttctg tcggtagtga agacggtcaa cgtcagatca caaacgtcgc
     4621 agctggttca gcggataccg atgcggttaa cgtgggtcag ttgaaagtca ctgatgagcg
     4681 cgtagcgcaa aatacccaga gcattactaa cctgaacaat caggtcacta atctggatac
     4741 tcgcgttact aatatcgaaa acggtattgg cgacattgtc accaccggta gcaccaagta
     4801 cttcaagacc aacaccgatg gcgtagatgc caacgcccag ggtaaagata gcgttgctat
     4861 tggttctggt tccattgctg ccgctgacaa cagcgtcgca ctgggtaccg gttccgttgc
     4921 agaggaagaa aatacaatct ctgtaggttc ttctactaac caacgccgta tcaccaacgt
     4981 agctgccagt gttaatgcca ccgatgcggt taacgtgtcg cagctgaaat cttctgaagc
     5041 aggcggagtg cgctacgaca ccaaagctga tggttctatc gactatagca atatcaccct
     5101 cggtggtggc aacggcagta cgactcgtat cagcaacgtc tccgctggcg tcaacaacaa
     5161 cgacgcggtg aactacgcgc agttgaagca aagcgcgcag gaaacgaagc aatacaccga
     5221 tcagcggatg gttgagatgg ataacaaact gtctaaaact gaaagcaagt tgagcggtgg
     5281 tatcgcttct gcaatggcaa tgaccggtct gccgcaggct tatacaccgg gtgccagcat
     5341 ggcttctatt ggtggcggta cttacaacgg tgaatcggca gttgctttag gtgtatcgat
     5401 ggtgagcgcc aatggtcgtt gggtctacaa attacaaggt agtaccaata gccagggtga
     5461 atactccgcc gcactcggtg ccggtattca gtggtaatga tccattaagt tagtgtgact
     5521 aagggcaaac gccaaattgc ctgatgcgtt acgcgcatca ggcaagataa ggcgctccgg
     5581 gtcaacaatc taagggcaat tctctgatga ggattgccct tttctttacc agacatctcc
     5641 ccccacaaga attggcccta ccaattcttc gcttatctga cctctggttc acaattcccc
     5701 aattaaaact cacatcaata ttgccaatac ataacattta gttaaccatt cattgtcatt
     5761 atccctacac aacacaattg gcagtgccac ttttacacaa cgtgtgacaa ggagatgagc
     5821 aacagactca ttacacgatg tgcgtggact ctaggagacc tgcaatgaat ctctggcaac
     5881 aaaactacga tcccgccggg aatatctggc tttccagtct gatagcatcg cttcccatcc
     5941 tgtttttctt ctttgcgctg attaagctca aactgaaagg atacgtcgcc gcctcatgga
     6001 cggtagcaat cgcccttgcc gtggctttgc tgttctataa aatgccggtc gctaacgcgc
     6061 tggcctcggt ggtttatggc ttcttctatg gtttgtggcc catcgcgtgg atcattattg
     6121 cagcagtgtt cgtctataag atctcggtga aaaccgggca gtttgacatc attcgctcgt
     6181 ctattctttc gataacccct gaccagcgtc tgcaaatgct gatcgtcggt ttctgtttcg
     6241 gcgctttcct tgaaggagcc gcaggctttg gcgcaccggt agcaattacc gccgcattgc
     6301 tggtcggcct gggttttaaa ccgctgtacg ccgccggact gtgcctgatt gttaacaccg
     6361 cgccagtggc atttggcgcg atgggcattc caatcctggt tgccggacaa gtaacaggca
     6421 tcgacagctt tgagattggc cagatggtgg ggcgtcagct accgtttatg accattatcg
     6481 tgctgttctg gatcatggcg attatggacg gctggcgcgg tatcaaagag acgtggcctg
     6541 cggtcgtggt tgcaggcggc tcgtttgcca tcgctcagta ccttagctct aacttcattg
     6601 ggccggagct gccggacatt atctcttcgc tggtatcact gctctgcctg acgctgttcc
     6661 tcaaacgctg gcaaccagtt cgcgtattcc gcttcggtga cttgggggcg tcacaggttg
     6721 atatgacgct ggcccacacc ggttacaccg cgggtcaggt gttacgtgcc tggacaccgt
     6781 tcctgttcct gaccgctacc gtaacactgt ggagcatccc gccgtttaaa gccctgttcg
     6841 catcgggtgg tgcgctgtat gagtgggtga tcaacattcc ggtgccgtac ctcaataaac
     6901 tggttgcccg tatgccacca gtggtcagcg aggctacagc ctatgccgcc gtgtttaagt
     6961 ttgactggtt ctctgccacc ggcaccgcca ttctgtttgc tgcactgctc tcgattgtct
     7021 ggctgaagat gaaaccgtct gacgctatca gcaccttcgg cagcacgctg aaagaactgg
     7081 ctctacccat ctactccatc ggcatggtgc tggcgttcgc ctttatctcg aactattccg
     7141 gactgtcatc aacactggca ctggcactgg cgcacaccgg tcatgcattc accttcttct
     7201 cgccgttcct cggctggctg ggggtattcc tgaccgggtc ggatacctca tctaacgccc
     7261 tgttcgccgc gctgcaagcc accgcagcac aacaaattgg cgtctctgat ctgttgctgg
     7321 ttgccgccaa taccaccggt ggcgtcaccg gtaagatgat ctccccgcaa tctatcgcta
     7381 tcgcctgtgc ggcggtaggc ctggtgggca aagagtctga tttgttccgc tttactgtca
     7441 aacacagcct gatcttcacc tgtatggtgg gcgtgatcac cacgcttcag gcttatgtct
     7501 taacgtggat gattccttaa tgattgtttt acccagacgc ctgtcagacg aggttgccga
     7561 tcgtgtgcgg gcgctgattg atgaaaaaaa cctggaagcg ggcatgaagt tgcccgctga
     7621 gcgccaactg gcgatgcaac tcggcgtatc acgtaattca ctacgcgagg cgctggcaaa
     7681 actggtgagc gaaggcgtgc tgctcagtcg acgcggcggc gggacgttta ttcgctggcg
     7741 tcatgacaca tggtcggagc aaaacatcgt ccagccgcta aaaacgctga tggccgatga
     7801 tccggattac agtttcgata ttctggaagc ccgctacgcc attgaagcca gcaccgcatg
     7861 gcatgcggca atgcgcgcca cacctggcga caaagaaaag attcagcttt gctttgaagc
     7921 aacgctaagt gaagacccgg atatcgcctc acaagcggac gttcgttttc atctggcgat
     7981 tgccgaagcc tcacataaca tcgtgctgct gcaaaccatg cgcggtttct tcgatgtcct
     8041 gcaatcctca gtgaagcata gccgtcagcg gatgtatctg gtgccaccgg ttttttcaca
     8101 actgaccgaa caacatcagg ctgtcattga cgccattttt gctggtgatg ccgacggggc
     8161 gcgtaaagca atgatggcgc accttagttt tgttcacacc accatgaaac gattcgatga
     8221 agatcaggct cgccacgcac ggattacccg cctgcccggt gagcataatg agcattcgag
     8281 ggagaaaaac gcatgattat ttccgcagcc agcgattatc gcgccgcagc gcaacgcatt
     8341 ctgccgccgt tcctgttcca ctatatggat ggtggtgcat attctgaata cacgctgcgc
     8401 cgcaacgtgg aagatttgtc agaagtggca ctgcgccagc gtattctgaa aaacatgtcc
     8461 gacttaagcc tggaaacgac gctgtttaat gagaaattgt cgatgccggt agcactggca
     8521 ccggtgggtt tgtgtggcat gtatgcgcgt cgtggcgaag ttcaggcagc caaagcggcg
     8581 gacgcgcatg gcattccgtt tactctctcg acggtttccg tttgcccgat tgaagaagtc
     8641 gcgccagcca tcaagcgccc aatgtggttc cagctttatg tactgcgcga tcgcggcttt
     8701 atgcgtaacg cgctggagcg agcaaaagca gcgggttgtt cgacgctggt tttcaccgtg
     8761 gatatgccga caccgggcgc acgctaccgt gatgcgcatt caggtatgag cggcccaaat
     8821 gcggcaatgc gccgctactt gcaggcggtg acgcatccgc aatgggcgtg ggatgtgggc
     8881 ctgaacggtc gtccacatga tttaggtaat atctcagctt atctcggcaa accgaccgga
     8941 ctggaagatt acatcggctg gctggggaat aacttcgatc cgtccatctc atggaaagac
     9001 cttgagtgga tccgcgattt ctgggatggc ccgatggtga tcaaagggat cctcgatccg
     9061 gaagatgcgc gcgatgcagt acgttttggt gctgatggaa ttgtggtttc taaccacggt
     9121 ggccgccagc tggacggtgt actctcttcc gctcgtgcgt tgcccgctat tgcagatgcg
     9181 gtgaaaggtg atatcgccat tctggcggat agcggaattc gtaacgggct tgatgtcgtg
     9241 cgtatgattg cgctcggtgc cgacaccgta ctgctgggtc gtgctttcct gtatgcgctg
     9301 gcaacagcgg gccaggcggg tgtagctaac ctgctaaatc tgatcgaaaa agagatgaaa
     9361 gtggcgatga cgctgactgg cgcgaaatcg atcagtgaaa ttacgcaaga ttcgctggtg
     9421 caggggctgg gtaaagagtt gcctacggca ctggctccga tggcgaaagg gaatgcggca
     9481 tagtcgtttg cgccccctca ccctaaccct ctccctcagg gagaggggac cgttcggcgc
     9541 tgtatgtact ccctcactct gaaacgacac cgcactcttt ttttctccct cgcccctccg
     9601 gggagggccg gggtgagggg aaaaggccgc acggttcacc aattttctgc tatcctgccc
     9661 ccgcactaag ggggcagtat gctaaacatc gtactttacg aaccagaaat tccgccaaat
     9721 actggcaaca tcatccgtct ttgcgctaat accggctttc gtctacatat catcgaaccg
     9781 atgggatttg cctgggacga caagcgcctg cgccgcgcgg ggctggacta tcacgagttt
     9841 accgccgtta cgcgtcatca tgactatcgc gcgttcctcg aagcagaaaa tccccagcgc
     9901 ctgtttgccc tcaccacgaa aggtacgcct gctcacagcg ccgtaagcta tcaggatggc
     9961 gattatctga tgttcggccc ggaaacacgc ggcctgccag cgagcattct tgatgccctg
    10021 cccgctgaac aaaaaattcg cattccgatg gtgccggaca gccgcagcat gaatctgtcc
    10081 aatgcggtgt cggtagtggt gtatgaagcc tggcggcagc tgggatatcc gggagcgttg
    10141 ttgagagatt agttactgta tgccggatag atgattacat cgcatccggc acgatcacag
    10201 gacattagat cccatcccca tactcaaatg tatggttaat accgttgaaa tgctggtcca
    10261 tatccattga tggcttatcg ctgtctggtt taccgacaat acgagccgga acgccagcgg
    10321 cggttgtatg cggcggcacc ggttgcagca ccacggaacc tgcgccaatc ttcgcgccgc
    10381 gaccaacttc aatattgccg aggattttcg cgcccgcgcc aatcatcaca ccttcacgaa
    10441 ttttcgggtg acggtcacca ccagatttac ccgtaccgcc aagcgtcaca gattgcagaa
    10501 tcgatacgtc gttttcaatt accgccgttt cacccacaac gatgcccgtg gcgtggtcga
    10561 gcataatgcc acggccaatt tttgctgccg ggtgaatatc gacctggaac gtcacagaaa
    10621 cctggttttg cagaaagatt gccaatgcgc gacgcccctg attccacaac cagtgaccga
    10681 tgcgataggc ctgcaaggca tgaaaaccct tcaggtataa caacggggtt gagtatttat
    10741 cgactgccgg gtcgcgggta cgcaccgcct gaatatcaca ggccgcagag gcgatcattt
    10801 ccgggtcagc ggcgtaggct tcttcaacca cttcacggat agcaatagca ggcataattg
    10861 gcgatgacag cttgttcgcc agcatgtagc tcagtgcact gccaaggttt tcgtgcttga
    10921 gtagcgtcgc gtggtaaaaa ctggccagca ttggctcaca gtccgccagc gttctggctt
    10981 cggctttaat attgttccag acaatttcca gttcttcaca cgacattgct tactccacac
    11041 gatgagataa tgaccggccc gttctgcgcg ggccgggtca tagcggtaac aaaggttccc
    11101 tggggttagt gactgctgcg ctcgtccttg cgtgcacgac ctaataatgt caatgctgcc
    11161 tcgcgcgcgt tttttccgca atataatact tgataaattt cctcggttat tggcatttca
    11221 acgccgaagc gatgcgccag ttcgcggact tctttcgtat tgcggtagcc ttccaccacc
    11281 tgaccaatct tctcctgcgc gctttgtaca tccatgccct gaccgagcat cataccaaaa
    11341 cggcggttac gcgactggtt gtcggtacag gtaagcacca gatcgcccag ccccgccatg
    11401 cccataaagg tggcaggatc ggcacccagc gccgcaccaa gacgcgacat ttcagccagc
    11461 ccacgggtga tcagcgccgt acgcgcattc gcaccaaaac cgataccgtc agacatcccc
    11521 gcgccaatgg caatgacgtt tttcaccgcg ccgccaagct gcacgccaat gaaatccggg
    11581 ttgctgtaaa cgcggaaact tttgccgcag tgcagcagct gctggagatc atcggcaaag
    11641 gtctgatcgg tcgaggccag cgaaatagct gtcggtaaac ctgccgccag ttctttcgca
    11701 aacgttgggc cagagataac cgccagcgga atttgatcgc ctaaggcctc acgcgccacg
    11761 tcctgtaaca gacgtccggt ttccgcttcc agccctttgg tcgcccacac cagacgcgca
    11821 tcaggacgca tcagcggttt aatctggcgc agcacttcac caaagacatg gctgggtacg
    11881 acgacgagaa tattacgact ggctgccagc gcagtggcga gatcgctttc aagatggagc
    11941 gtatcgggaa aaggcacatc ggggagaaac gcggcgttac agcggtcgcg ttcaagcgtt
    12001 gcgatatgtt cagggtcatg gccccagagg acaacctcgt ggccatttct tgccagggta
    12061 atggcaagag cggtgccgta cgagccggca ccgatcacag tcattgaagc attacgttgg
    12121 ttcatcaggc atcctgatgt tcttcagtac cttcgccagc ctgctgctgc aaatagttca
    12181 tgaacagcgc atcgaagtta accggcgcaa ggttcagttg cgggaatgta ccgcgggata
    12241 ccatgctggt gatgcactca cgagcatacg ggaacagaat gttcgggcag tatgcgccca
    12301 ggcaatgtgc catctgggtg ccttcgatac ccgcgatgga gaaaataccg ccctgctgaa
    12361 cttcacacag gaacgcggtt tcttcgccca aggaggccgt tacggtaaca cgcagtacca
    12421 cttcgtatac gtcatctgcc agttgggaag atgccgtatc cagatcaagt ttaacttctg
    12481 gttgccaatc tttctggaaa acgtgcggcg cgttcggcgc ttcgaaagag atatccttgg
    12541 tataaatacg ttggatctgg aaagtcattt cagtgttgtt ttgttctgac atgtgtagaa
    12601 aacccttaag tgttgtcctt aaatactgcg caatgtaata ccaacgttca cacgttattt
    12661 cagcagggga tccagtccac cacgtgcatc caatgcatac aagtcatcac agccgccaat
    12721 gtgctgtgcg tcaataaaaa tctggggaac cgtggtgcaa ccgctgcgtt tgatcatctc
    12781 ttcacgcttg gcggcattgc catcgatcgg caactcctgg aaactcacgc ccttgctgct
    12841 cagcagtgct tttgcacgat ggcaatacgg gcaggtttct ttggtataga tttcaacatt
    12901 ggccataatt tagctcctgt ttttttaccc tgaaatttca tgttgcaggg cggctgcaaa
    12961 atggagtgtc cccagaagct tataacaata agtcgctggg ggcctaattg cagctaacgg
    13021 cctgcatcat gaaagacgac aggtaaatta tttgccgcgc accagaggca agttttcgcc
    13081 agcccagcca gcaacgccct ctttcagcac gaatacttgc gcaaaaccag ctttcgtcag
    13141 cgcgtttgca ggctcctggc actgcatgcc agaaccgtct accacgataa ccgatttgtc
    13201 tttgtgcttc tcaagctcac caacattgtt ggctttgatt tcgctcggca acagattgat
    13261 agaacctgcg atatggcctt tacggaagtc atcacgctga cgtaaatcca caaccacagc
    13321 gtcttctttg ttgatcagac gcgtagcttc accacgagta atcaccttca ctttcgaggt
    13381 caggctctta aacgtagtca caagaaccgc caccagtaac gcgatccagg cgatactcag
    13441 tatgggatga cggccaacaa attgcataat ttcttgcatg gggggtaaca actcccgacg
    13501 tagtgattaa aaaaaccagg aaaggagtat acctgcgcgg tgcggcaaat acagccagcg
    13561 cgttaactgg aatgcaattt tgcggggcac gacgaaaaaa aagcgcaaat ctggcatcgg
    13621 cttgccgcga gcggtcgtat attttgatct tcagaggcta ttttatcgat tcagctgtag
    13681 taaaattacg caaattttga ctcttgagta tgaggttgtc gcaatgtcgg tttctaaaaa
    13741 acctatggta ctggtgattc tggatggcta tggctatcgt gaagaacagc aggataacgc
    13801 catttttagt gctaaaaccc cggtaatgga tgcactgtgg gccaatcgtc cgcataccct
    13861 gatcgacgct tccggtctgg aagtcggtct gcctgaccgt cagatgggta actccgaagt
    13921 aggccacgtt aacctgggtg ccggccgcat cgtgtatcag gacctgactc gtctggacgt
    13981 tgaaatcaaa gatcgcgctt tctttgctaa tccagtgctg gctggcgcgg tacataaagc
    14041 gaaaaacgca ggtaaagcag tacacattat gggtctgctc tccgcaggcg gcgtacacag
    14101 ccacgaagat cacatcatgg cgatggtaga actggcagct gaacgcggcg cagaaaaaat
    14161 ctacctgcac gcattccttg acggtcgcga caccccgccg cgcagtgctg aatcctcgct
    14221 gaaaaaattt gaagagaaat ttgctgcgct gggtaaaggc cgcgtagcgt ccatcattgg
    14281 tcgttactac gcgatggacc gcgataaccg ttgggatcgc gttgaaaaag cctatgacct
    14341 gctgactctg gcgcagggtg agttccaggc cgatactgcc gttgctggtt tgcaggcagc
    14401 ttatgctcgc gacgaaaacg acgagttcgt gaaagcgacc gttattcgtg cagaaggtca
    14461 gccagatgcg gcaatggaag acggcgatgc gctgattttc atgaacttcc gtgctgaccg
    14521 cgcgcgtgaa atcactcgtg cattcgtgaa cgctgatttc gatggcttcg cgcgtaagaa
    14581 agtggttaac gtcgatttcg tgatgctgac cgaatacgcc gctgacatca aaactgcggt
    14641 tgcttaccca cccgcttccc tggttaacac cttcggcgag tggatggcga aaaacgacaa
    14701 aactcagttg cgtatttccg aaaccgaaaa atatgcccac gttactttct tcttcaacgg
    14761 tggcgtagaa gagtcgttca aaggcgaaga tcgcattctg atcaactcac cgaaagtggc
    14821 tacctacgat ctgcaaccgg aaatgagctc cgcagagctg accgaaaaac tggttgcggc
    14881 catcaagagc ggcaaatacg acaccattat ctgtaactat ccgaacggcg acatggtagg
    14941 tcacaccggg gtgatggaag cggcggttaa agcggttgaa gcactggatc actgcgtgga
    15001 agaagtcgcg aaagcggttg agtccgtggg tggacaactg ctgatcaccg ctgaccacgg
    15061 taacgctgaa cagatgcgcg atccggcaac gggtcaggca cacacggcac acaccaacct
    15121 gccagttccg ctgatttacg ttggtgataa gaacgtgaaa gcggttgcag gcggcaaact
    15181 ttctgacatc gcgccgacca tgttgtcgct gatgggtatg gaaatcccgc aagagatgac
    15241 tggtaagccg ctgttcatcg tggaataatc cctccccatg aggggaaagg cgattaatac
    15301 catgacacgg gccgtgaaac cgcgcaggtt tgcaatcagg cccatcatct acgccagcgt
    15361 tcttagcgct ggcgtattgt tgtgcgcctt ttccgcccac gcggatgagc gtgaccaact
    15421 caaatctatt caggccgata tcgccgcaaa agagcgcgcg gtacgccaaa agcaacaaca
    15481 acgcgcaagc ctgctcgcac aattgaaaaa gcaggaagaa gcgatctctg aagccacccg
    15541 taagctgcgc gaaacgcaaa acacgctcaa tcaactcaat aaacagattg atgagatgaa
    15601 cgcgtcgatt gccaaactgg agcagcaaaa agccgcccag gagcgcagcc tcgccgcaca
    15661 actggatgcc gcattccgtc agggcgagca taccggtatt cagctaattc tcagcggtga
    15721 agaaagccag cgtggacagc gtttacaggc ttatttcggc tatctcaacc aggcgcgaca
    15781 agaaaccatt gcccagttga agcaaacgcg tgaagaagtc accatgcagc atgctgaact
    15841 ggaagagaaa caaagcgagc aacaaacgct gttatatgaa cagcgcgccc aacaggcgaa
    15901 actgactcag gctctgaacg agcgtaaaaa gacgctggcg gggctggaat cttccatcca
    15961 gcaaggtcag cagcagctaa gtgaactgcg tgccaacgaa tcccgtctgc gtaacagcat
    16021 tgcccgtgct gaagccgcgg cgaaagcgcg tgctgaacga gaagcgcgtg aggcccaggc
    16081 ggttcgcgag cgccagaaag aagcgacgcg caaaggcacc acctacaaac cgaccgaaag
    16141 cgaaaaatcg ctgatgtccc gaactggtgg cctgggtgcg ccgcgtggtc aggcattctg
    16201 gccggttcgc gggccgacgc tgcatcgcta tggcgagcag ctacagggtg aattacgctg
    16261 gaaagggatg gttatcggtg cctctgaagg tactgaagtt aaagcaattg ccgacggtcg
    16321 agtgattctg gctgactggc tgcaaggtta cggtctggtg gtggtggttg agcacggtaa
    16381 aggcgacatg agtctttacg gctataatca gagcgcactg gtgagcgttg gttcgcaggt
    16441 tcgcgcgggc cagccaattg cactggtggg cagcagtggc ggtcagggtc ggccttcact
    16501 ctatttcgaa attcgccgcc agggtcaggc ggtcaatcca cagccgtggt tgggaagata
    16561 agttttgttt ccatttcgtc gtaacgttct tgcatttgcc gctctgttgg cgctctcctc
    16621 ccccgtactt gctggcaaac ttgccatcgt cattgatgat tttgggtatc gcccgcacaa
    16681 cgaaaaccag gtgctggcga tgccttccgc tatctccgtc gctgtattac ccgattcacc
    16741 gcacgccaga gagatggcga ccaaagcgca taacagcggt cacgaagtgt tgattcatct
    16801 cccgatggca ccgttgagta aacagccgct ggagaaaaat acgctacgcc cggagatgag
    16861 cagcgacgaa attgagcgca ttattcgtag tgcggtcaat aacgtgccct atgccgtggg
    16921 gatcaacaac cacatgggca gcaagatgac ctctaacctg tttggtatgc agaaagtgat
    16981 gcaggcgctg gagcgttaca atctttactt cctcgacagc gtaaccatcg gtaataccca
    17041 ggcgatgcgc gccgcgcaag gtactggcgt gaaggtgatc aaacggaagg tgttcctcga
    17101 cgattcgcag aacgaagcgg acatccgtgt gcaatttaat cgcgcaattg atctggcgcg
    17161 tcgcaacggt tcaaccattg ccattgggca tcctcaccct tcaacggtac gcgtgctaca
    17221 acagatggtt tataacctgc cgccagacat tacgctggtg aaagccagca gcttgctgaa
    17281 tgaaccgcag gttgatactt ctacaccgcc gaaaaacgct gtgcctgatg caccgcgtaa
    17341 tccgttccgt ggcgtgaagc tgtgcaaacc gaagaaaccg atagaacctg tttatgctaa
    17401 tcgcttcttt gaagtattaa gcgaaagcat cagccagagc acgctgatcg tttacttcca
    17461 gcatcagtgg caaggctggg gcaaacagcc cgaagcggcg aagtttaacg ctagcgcaaa
    17521 ttaaaggcgc tacgcgtaat gcgatggtgc gaccgcgttt tgtcgcgcca ttgccataag
    17581 cggaaagacc acagtaacgc ctggtaaccg accttcacgc tgcgcacatt ggtaatcagg
    17641 cgcttataca tacccggaag taaatatctc ggcaatcatc cgttggcggg taagaatatc
    17701 cggctctttg cgc
//
LOCUS       DAZXUK010000064        17169 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZXUK010000064 DAZXUK010000000
VERSION     DAZXUK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17169
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.064"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(42..773)
                     /gene="rlmB"
                     /locus_tag="ACH3L8_001705"
     CDS             complement(42..773)
                     /gene="rlmB"
                     /locus_tag="ACH3L8_001705"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFT4084858.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(953..3394)
                     /gene="rnr"
                     /locus_tag="ACH3L8_001706"
     CDS             complement(953..3394)
                     /gene="rnr"
                     /locus_tag="ACH3L8_001706"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFT4084859.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="ACH3L8_001707"
     CDS             complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="ACH3L8_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFT4084860.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4063..5361)
                     /gene="purA"
                     /locus_tag="ACH3L8_001708"
     CDS             complement(4063..5361)
                     /gene="purA"
                     /locus_tag="ACH3L8_001708"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFT4084861.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5465..5662)
                     /locus_tag="ACH3L8_001709"
     CDS             complement(5465..5662)
                     /locus_tag="ACH3L8_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFT4084862.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="ACH3L8_001710"
     CDS             complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="ACH3L8_001710"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFT4084863.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="ACH3L8_001711"
     CDS             complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="ACH3L8_001711"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFT4084864.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="ACH3L8_001712"
     CDS             complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="ACH3L8_001712"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFT4084865.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="ACH3L8_001713"
     CDS             complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="ACH3L8_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFT4084866.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="ACH3L8_001714"
     CDS             complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="ACH3L8_001714"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFT4084867.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="ACH3L8_001715"
     CDS             complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="ACH3L8_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFT4084868.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12646..13983)
                     /gene="amiB"
                     /locus_tag="ACH3L8_001716"
     CDS             complement(12646..13983)
                     /gene="amiB"
                     /locus_tag="ACH3L8_001716"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFT4084869.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14002..14463)
                     /gene="tsaE"
                     /locus_tag="ACH3L8_001717"
     CDS             complement(14002..14463)
                     /gene="tsaE"
                     /locus_tag="ACH3L8_001717"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFT4084870.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14435..15982)
                     /gene="nnr"
                     /locus_tag="ACH3L8_001718"
     CDS             complement(14435..15982)
                     /gene="nnr"
                     /locus_tag="ACH3L8_001718"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFT4084871.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKPLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15981..17120
                     /gene="queG"
                     /locus_tag="ACH3L8_001719"
     CDS             15981..17120
                     /gene="queG"
                     /locus_tag="ACH3L8_001719"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFT4084872.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17103..17156)
                     /locus_tag="ACH3L8_001720"
     CDS             complement(17103..17156)
                     /locus_tag="ACH3L8_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084873.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3865 a         4921 c         4327 g         4056 t
ORIGIN      
        1 aaaccatcca aatctggatg gcttttcata attcagagga actagctgcg ctgacgaacc
       61 gcttcaaata agcaaattcc ggttgcaacc gaaacgttca gggaagaaac acttcctgcc
      121 attgggatgc tgatcaactc atcgcaatgt tcacgggtca ggcgacgcat accttcacct
      181 tccgcgccca tcaccagcgc caggcgtccg gtcattttgc tctgatagag tgtatgatcc
      241 gcctcgcctg ccgtaccgac gatccagata ttctcttcct gcaacatacg catggtgcgc
      301 gcaaggttag tcacccgaat cagcggaacg ctttctgccg cgccgcaggc tacttttttc
      361 gccgtagcgt tgagctgtgc ggagcgatct ttcggcacaa tcaccgcatg aacgcctgcg
      421 gcgtccgcac tacgcaggca cgcgccgagg ttgtgcggat cggttacgcc gtcgaggatc
      481 agcaggaacg gttgatcgag cgaagcgatc agatccggca gatcgttttc ctgatactga
      541 cgtcctggct tcacgcgggc gataatgccc tgatgcacgg caccgtcgct tttctcgtcg
      601 agatattggc ggtttgccaa ctggataacc acgccctgag actcaagggc gtgaatcagc
      661 ggtaacagac gtttatcttc acggcctttt aaaataaaga cttcctgaaa acgttcaggg
      721 gcgcgctcca gcagggcctg cactgcgtgg atgccgtaaa tcatttcgct cattaatgta
      781 ctcgttgttt acgttttgcc tgatgcacta cgtttatccg gcctacatga tctctgcaat
      841 atattgaatt tgcgtgatta tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
      901 caaagcgcat tttgtcagca atctaaccct cttcttttaa agagggtatt gatcactctg
      961 ccactttttt cttcgccgca cgcttcgctt tggtcgctgc ggctattttc tgcgttttcg
     1021 ccgatggctt tttcgctttt ctcgcgtctt tcttcgccgc tttcggcttc gtttttttct
     1081 caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgacctga caacgcttgc
     1141 cgcctttttt gcctgcatcg ccttttttcg ctttctcgcg cgccgtttta ccgacgttgc
     1201 gcggtgcgcg ttcactggag atcagactaa agtcgatttt gcgctcgtcc atattaaccg
     1261 cttcgacgcg aacttccacg cgatcgccca ggcgataagt ctggccgctg gattccccca
     1321 tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa gagacatgga
     1381 ccagaccatc aatgaacaag tcgtccagac ggacgaagaa gccaaagcca gtgacgctgg
     1441 aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac ttcagccagt
     1501 cagcgacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag tgctgaccca
     1561 gttgcaacat ctcttccatc gaataatggt agccgccggt ttcagtggtg ttgccctgat
     1621 gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg tcaggataac
     1681 gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc cagaccgaag tgaccacggt
     1741 tttctggatc gtaaatcgcc tgtttcatcg agcgtagcag catggtttgc agcatttctg
     1801 catcaggacg gtcggcaacc gactccagca gttccgcgta gtcacgcggt tccggcttgt
     1861 taccacccgg cagctccagc cccagctccg ccagcactga acggaaagag gtaatcgctt
     1921 cggtgctcgg cttgtcgtga atacggaaca gtgccggttc tttcgctttc tcaacgaaac
     1981 gcgccgccga gatattcgcc agaatcatgc actcttcaat taatttgtgc gcgtcgttac
     2041 gctgggtctg ttcgatacgt tcaatacggc gttcagcgtt gaaaatgaac ttcgcttctt
     2101 cgctctcaaa tgagatccca ccgcgttctt cacgggcttt atccagcact ttatagaggt
     2161 tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg ctcgcgcaga tcctgatcgc
     2221 cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc atcaccgctt
     2281 cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg cacaccatac
     2341 acaggcggtc tacctgcggg ttgagcgaac acaggccgtt agagagcact tccggcagca
     2401 tcgggataac ctgcgaaggg aagtacaccg acgtgccacg gttacgcgct tctctgtcca
     2461 gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat aaacgccagc
     2521 cgccgccgcg ttttttctcg cagtaaactg catcgtcaaa gtcacgggcg tcttcgccat
     2581 caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct tcttccggca
     2641 cttcttcttt cagccccgca acctgttgct caacagcctg cggccagata tacggaattt
     2701 catgggtacg cagagcgata tcaaccgcca tgccggtgcc catattgtcg cccagcactt
     2761 cgacgatttt acccaccgct ttggtgcggc gagtcggacg ctgagtcagt tcgactacga
     2821 ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata tcgaagctca
     2881 gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag cgaccaacaa
     2941 tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga cctttacggt
     3001 cagcacccag cggctgcgcc agcacctgat cgccatgaat gcaggttttc atctgctcgc
     3061 tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag ccatcacggt
     3121 gaccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag cactgacgac
     3181 gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc aggccttcaa
     3241 gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg gccggttttt
     3301 cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc gcgtattttt
     3361 cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg tcatctctga
     3421 tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct cttcaaccaa
     3481 atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct tagaaagcgc
     3541 ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat tcaccagcga
     3601 taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg gtttacccag
     3661 gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac taagttgatt
     3721 gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt cagaaatact
     3781 ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc cgtaatcagt
     3841 gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata catttatatt
     3901 gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga aggaaaaaag
     3961 cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca atgtatcaca
     4021 ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt cgaacgggtc
     4081 gcgcagaatc atggtttcag tacgatccgg accggtagag atgatatcga tcggcacacc
     4141 ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcaggc cgctacgatc
     4201 tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg gctctacacc
     4261 tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg gcatacggta
     4321 agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag tcaggcagaa
     4381 gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca gccagccggt
     4441 acgacgacga cgacccgtag ttgcaccgaa ttcgttaccc tgcttgcaga ggaactcgcc
     4501 agtttcatca aacagttcgg ccgggaaagg acctgcacct acacgagttg agtaagcttt
     4561 gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg tcgccacgcc
     4621 accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga tatccagcag
     4681 cgtaccctgc gcaccttcga acatgacgaa atcgccacgc tgacgcgcct ggtcgagcag
     4741 gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg tatcatccag
     4801 aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga agttgtgata
     4861 ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt cgccaacacg
     4921 cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac cggtggtgcc
     4981 gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agagcaacgt gataatcaag
     5041 gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc cacggtcttc
     5101 cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt taccgatgat
     5161 gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg ttttttcacc
     5221 gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa catatttagc
     5281 ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt gggtgcccag
     5341 tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg gattctacca
     5401 tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc agtctgtttt
     5461 ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca accacaagtc
     5521 caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag atcatcttct
     5581 tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc agggcaagcg
     5641 ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca ccgacatccc
     5701 tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc gttgcggaag
     5761 tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc acgtcctgat
     5821 tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag aagtccggat
     5881 ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca ccacgcataa
     5941 tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg gcagtcgcgc
     6001 gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc gcttcacgct
     6061 cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg atctgcttga
     6121 tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg atgaccggaa
     6181 ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg ttatctgctg
     6241 ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg tcacgtactt
     6301 cgagggtcag acgaccacgg gaatcggtga cgatatcttt cacgtccagg cgaccaattt
     6361 cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc gaaatgtcgc
     6421 caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg atgtaagagt
     6481 cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg tccatggtct
     6541 gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa tgcagaccag
     6601 gctcataaac caaaggtttg ttgtcatcgt cacgcagtac cttaccaaaa cgcagcgtaa
     6661 taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc agcacgatga
     6721 tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt agtcgttacg
     6781 ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg acgtgttgct
     6841 tgctccactg gttgtggagg aagaggctgg cggcagacgc agcagattgc tggcaccgtt
     6901 atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg gcagaaccat
     6961 caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca ctttttccat
     7021 cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt ccggcagaag
     7081 tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct tgtacgcacg
     7141 cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt cgttggaata
     7201 cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg catcgtcaaa
     7261 cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca gcagcgtgat
     7321 acccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgagtatcgc tacgaatcac
     7381 ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataactc cacgcagggc
     7441 gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca gatatttttc
     7501 cggattggtg acgcggtact gcacgttcat ctcaacgcgc actacgttct cgtccgacgt
     7561 cagcatcaca ccagaagcgg ccagttcacg cacggcttcc acgttcaccg gtttgacttc
     7621 gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga atttaccaaa
     7681 gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg ccgcccagat
     7741 aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg ggcgcgggcc
     7801 ttgtgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac caccgagctt
     7861 tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc gaccgccttt
     7921 gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt cgcggtcttg
     7981 tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt atgcggtgat
     8041 ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta cgccgttaga
     8101 tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca acgatcggca
     8161 tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct attgcctgaa
     8221 gctgataaaa acgactcctc agacgccctt cctgcggtgg cagacgcaat gtatgctgcg
     8281 ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc cccgctccgg
     8341 tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca atacgcggtt
     8401 cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc tcgtgagcgt
     8461 cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc acatccgccg
     8521 catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg gctttaaatg
     8581 ccgccaccag atcgtgcggc aggtggcgaa taaaccctac ggtatctgca agtacggttt
     8641 caccgacatc tgcaacgtca atacgccgca acgtcgggtc gagggtggca aacaactggt
     8701 ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta ccggcgttgg
     8761 tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc cgcccctgct
     8821 cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga ttacgcaaca
     8881 aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc ccgcctttct
     8941 gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc agctgcgcca
     9001 gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata tctaaaataa
     9061 ggccggtgcg gtcgataaca cgacactcgc acaaacgctc caggttacgc tcttgcgccg
     9121 ggctcagggc atggtcaaaa agaacgaccg aagcacccat cgctttgaca gcttccgcaa
     9181 tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta cggctaccgg
     9241 taatcacctg caatgcttcg acaccggcgg aagagaccag agattcaaac tcctggaggt
     9301 cttccatatc tttgtcttgc gtaaaataga tgtgtaccag taccgcctgc tcaccagcat
     9361 cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg caggatcgct
     9421 ggctccccgt gtaaaaaaac agcccgaaac cttattcggt ttcttcgctg tcctgttgcg
     9481 cggaagtatt ctgcgcgctg ctaccatgat ggtagttact gctggtaccg ccaccggcgt
     9541 tgttactgtg atgagaaacc gggcgagatg ggacaacagt agaaatcgcg tgcttgtaaa
     9601 ccatctggct gaccgtgttt ttcaacagga tcacgaactg atcaaaagac tcgatttgcc
     9661 cttgcagctt aataccattc accaaataaa tagaaactgg aacacgttcc cgacgcagtg
     9721 cgttcaggaa cggatcttgt aaagattgcc ccttagccat tctctctttt ccttatatgc
     9781 ttatttgtac tttgaacctt tcgattctga aaaaattgcg cacgatacgt ctcaattgta
     9841 cacattcagc ctgcgatagc accaacaacc tgtaatactt cgtcacgcgc ctgttctggt
     9901 ttttcactgt caagccagtg aaccccttcc caaccacgca gccaggttat ctgccgcttc
     9961 gccaactgtc tcgtggcgca aacacctcga taaaccattt catcgtatga gatttcgcct
    10021 tcaaggtaag accacatctg gcgataaccc acgcaacgaa tggaaggcaa gtccgtatgc
    10081 aaatctcctc gggcaaaaag cgcccggact tctgcttcaa aacctgaagc caacatctga
    10141 tgaaaacgct gctcaatgcg ttgatggagc agttcacggc tcgccggggc gatggcgaac
    10201 tgatgcacct gatacggtag agcgtctcct gacgtttgcg tcagttccgt taaagtttta
    10261 cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt gtggatcatt tggatgaatc
    10321 cttgctgccg caaccggatc tacctcctga agttgacgat gcaatgactc ccaaccttgc
    10381 tctgccgcct gttgctcaat tctggcccgc acttccgggt ctgccgacgg tagcggcgac
    10441 aacccttcca gcaatgcctt gaaatacaac atcgtaccgc ccactaacag tgggatccgc
    10501 cccgccgcgg tgatatcggc catttccgcc agcgcatcgc ggcgaaaatc agcagccgag
    10561 taagcctgcg acggatcgcg aatatccagc aatcggtgcg gcgcggcgag taactcttca
    10621 gcgttcggct tcgccgtccc gatatccatc cctttgtaaa taaggacaga atcaacgctt
    10681 atcaactcta ctggtaaaat tttacgcagc tcaatggcta acgccgtttt accggaggcc
    10741 gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct tactgatatc actcatcttt
    10801 cagggctttt atcgccggat gtaaatcaac agattgtaac agaccacccg gcggcgtttt
    10861 cacaagttgc ggacataacc gttccacgtc cgccagcagg gttatggcct gtgccattga
    10921 ccactgcgca tgttcgctca tcagatttcg tgcaatccac tgcgcaatat tgccaggttc
    10981 gaatacggac tgcttcgcca ggtagcctat cagttcagga atcaagattt gtaaattttg
    11041 ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc tgtgcatctg actggaaatc
    11101 aatacccaat tccgctaggg cagactgcgc tttttctaat gccgattttt cttcgccaga
    11161 aacttttagc cgcaacggaa tcagcagcgg ctgggcgcaa acgggcgctt cacccggcgt
    11221 caattgtgcc tgacgcagcc aacgttctgc cactggcaag gataaaagtg aaatgttgcc
    11281 atcgcgctcc agcaacgcac agtcggaatg gacgatagtc agtacccgac caaaactctg
    11341 actgttcgcc gcaagtgcag gttcctgcgg ttccggcgct tttggttttt gcatcggcgc
    11401 gggcgtttgc aaaagctggc gatacacttc accttgctgt ttctggtagc ctggctgcgc
    11461 attcggccag ggggcagccg gacgactgcc tgaggcgggc gcaggagagt agcgcggagc
    11521 taccggctca cgagctgccg gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc
    11581 cgggatggca cgcggtgcgg gttgtggttc atcgtccagc ggtagcggcg tttccagttg
    11641 ctgttgtagc acgctcagca cgccctgata gataaaatca tgcaccagac gcgactgatg
    11701 gaaacgcact tcgtgtttgg cggggtgtac gttgacgtcc acctgatgcg ggtcgatctc
    11761 cagatacaac acaaatgccg gttgctgatc ggcccccagt ttgtcttcgc aggcctggcg
    11821 gatcgcatga ttgatcaggc gatcgcgcat catgcgacca ttcacgtagc aatactgaat
    11881 ttccgccagt gcgggcgtag tgtgatttgg atcggccacc cagccgcgca gggtgagatc
    11941 gccgtgttgc cattcaatcg ccagcgcttg ttcgagaaaa gcggtgccgc aaatcgcgcc
    12001 taagcgccgt tctttttgcc cgccttccgg cactgcgcgg tactgacgca caattttacc
    12061 gttatgcgac aggttgatcg tgacgtcgaa acgcgccagc gcaatgcggc ggatgatttc
    12121 atcaatatgg ctaaattcgg ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt
    12181 gtagaacaga tccagcacct ccagcgtcgt tcccaccgga tgcgctgccg gtttaaccgt
    12241 cacgtccatg tcgcgtcctt cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg
    12301 tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc
    12361 caggctgata atggcttcga gatcgtccag agaggcgatt ttactggtgg catgacgagc
    12421 cagcgccagc gccagctcat cttttttgat accgcagccg ttatcacgaa tgcggataag
    12481 tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt
    12541 ttccactagt tctttgacta ccgacgcagg tcgctcgacc acctcacctg cggcaatctg
    12601 gttcgccagt tgtggcggta agacctgaat tggcatcaat cgtccttagt ttggcagcgt
    12661 gcgatctggc gtcgtcaccg tactggcagt ttgtgccgtt gcaccctgcg gcgcagattg
    12721 catcggatgc gcaaggaagt aattgcgcag gcctttgtaa atggcttctg ccagctgttg
    12781 ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc
    12841 gaccagtact gatgggatat ccggcgagcg cagaacgcca aggctggcgt gttctggtcg
    12901 acgtttatgt atttcgccaa tgcgttgcaa ctgactgatc atactggtcg ctacatcata
    12961 ccctacccgc tgggaatgac cgaactgtaa atccagcacc gcctggctta aataggggtc
    13021 agactgactg ttcgccagca catcacccgc cccacccagc agctccgaca gtttctcgtg
    13081 ctgctccagc cagctggcca tttcactgtt ggcgcgacgg ttagagagca cccatacgga
    13141 agcgccagtc gcactacggt tcggtgcggc atcagcgtga atcgacacga ggaaattggc
    13201 gttttgctta tgtgccacat cactgcgccc catcaccgag ataaagtaat ccccgtcacg
    13261 ggttaaaacg cctttaaaca tcggatcgtc attgagcaaa gtacgcaatt tacgcgcgat
    13321 ggcgatggtg acatttttct cccgcgtacc accggggccg atagcgccag ggtcctgacc
    13381 gccgtgtccg gcatcaatag cgatgataat tttatcgcca gtgttagccg tcgcgcgcgc
    13441 tgccggacgc gttaccgtat tactgctgat aacacccgta gtgcggttac tttccgtttt
    13501 aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg
    13561 tttcgcaacc acgggcggcg gaggaggcgg tggcggcaca tcggcgttaa tcgtaaagac
    13621 gacagtgtaa ttgctgccat tctgccgctt caccgcttcg gttttaccgt tttcggtaag
    13681 atcgaccacc agccgtagcg tttgtgcatc tttaggcgtt ccagagcgaa tcgccttcac
    13741 cagattattg ccgctgaaca acaacggcag tccctgaatc atgcccgttt gtttgatatc
    13801 gagcgccacg gtgcgtttgc tttgatggct aaacgcataa tcaggatcgc caataaaact
    13861 caacgttatc cgcgcctgtt gattaccgtt agaaacctga atatcagaga gcgtcgcggc
    13921 acccaccggc gtgcacagca gcagcagcgt cgctaccaac caatttctga tgcgatacat
    13981 catcccgcca cctttcaaag gttaaccggc taaacgcgcc agcaacaatt cacccgcaga
    14041 ggaaaccgca ctcacgcgcg cctcacggcc ttgtgcctga taatcaatgt gtatttcgac
    14101 atccgggtca ggaagaacac ctgtaccttg ttgtggccac tccaccaggc agatggcatc
    14161 gttggcaaaa taatcgcgga tccccataaa ctccagctcc tcgggatcgg caaggcggta
    14221 caaatcaaag tgatagacca ttaagttgtc gagcgtatag ggttcgacca gcgtataagt
    14281 ggggcttttg acattaccct gatgacccag agcctgtaaa aagccccggc taaaggtggt
    14341 tttacctgcg cctaaatcgc catacagata gattacggtt gcgccatcgc aggctttcgc
    14401 tacccgctcg cccaggtcta atgttgcctg ctcatcaggg agcggaatta ctcgattcat
    14461 catggttttt atcagtcact tccgggttaa caatacgctg tagcgtggaa aagagatcgg
    14521 ttgccagcat cccgcgcgtt ccaaaacgcg ccgccagtac gtcagctgcc gcaccgtgcg
    14581 cgacacagcc tgcacaggct gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa
    14641 taataccaga gagcacatcg cccatgccgc cgctcgccat gcctgcattt ccggcatcaa
    14701 taatgcctaa agcgtcagga tgggcggcga ccacggttcc ggcacctttc agcaccgcta
    14761 cgccgccata acgttgtacc agaggtttgg cgcaatgtaa gcggtcactt tcaatttcag
    14821 cgacggaaca gcctaacaac cgtgcggcct cgctaggatg cggcgtgatc acgcgattgt
    14881 gacgcttatc gggattgatt gccagcaggt tcaatgcatc ggcatcccac aacatcggtt
    14941 tgcgaaaatt ctcaactttt tgcagggctt ttttccccca ctcttgctgg cccagaccgg
    15001 gaccaatcac caccacatcg gcccattcca ggctttcggt aagagagtcc atagtcagtt
    15061 catgcaccat caattctggt cgtgcagtca gcagcggcgc aatgttctca ctgcgggtca
    15121 gtactcggac taaaccagca ccagcacgta gcgccgcttc ccccgtcata cgaatagccc
    15181 ccgccgtgcc gtgatcgcca ccgataatca ccagccgccc gtgatcgcct ttatgcgaag
    15241 tcgggcgacg cggtttcagc cagtgagaaa gttgttctgc cgaaaaccgc tgaattttcg
    15301 tctcctgacc tgccagccag ctatccagcc ccagtgagtc aaaatgcagt tgtcctgtaa
    15361 catcccgcgc ttttccagtg agcaagccta gtttcagcgc aataaaagtg atggtgtgat
    15421 cggcgttgat cactgcgcct ggcgtagcgc cggtttcagc cagcaggccg gaagggatat
    15481 caaccgccac aatcggcgca ggatgggaat tagcgtggtc gattaactgg ctaatggatt
    15541 cgcggggcgc ttgctgcaag ccggtaccga gcagcgcatc aacaatcaga tctaccgatt
    15601 cgggccagac aatattcgaa gcatggatct cgccacccgc gtttaaccat gcttcgcgtg
    15661 ccagcgcggc ctcttccggc aacggtttgt cgctctcctg ggccaacaac gtgacctcaa
    15721 tgccgaccgc tttggccagt cgcgcgacca cgtagccatc gccgccgtta ttaccatgac
    15781 cgcacagcac cagccagtgg cgggcgtcag gatacgccga acgacacacc tggaatgcgg
    15841 cctcgccagc gcgaagcatc agctcataga gtgtgagccc cagcacatct gccgcctcgc
    15901 gttctccgcg gcggatatcg tcggcgtacc agacggtgtg tggtatactt acggggtttt
    15961 tcttcattgt atggtccgtc atgtcagagc ccctcgatct caatcagtta gcgcaaaaaa
    16021 ttaaacagtg ggggctggaa ctgggctttc agcaggtagg tattaccgat accgatctca
    16081 gcgagtccga gcccaaactg caagcatggc tggacaaaca ataccacggc gaaatggact
    16141 ggatggcacg tcacggtatg ctgcgcgctc gcccccatga gttattaccc ggtacgctgc
    16201 gtgtaatcag cgtgcggatg aattaccttc ctgctaacgc cgcatttgcc agcacgctga
    16261 aaaaccccaa actcggctat gttagccgtt atgcgctggg ccgtgactat cacaaacttc
    16321 tgcgcaaccg actcaaaaag ctgggcgaga tgattcagca acattgtgtt tcgctgaatt
    16381 ttagaccgtt tgtcgattct gcgcctattc tcgagcgccc gttagctgaa aaagctgggc
    16441 tcggctggac aggtaagcac tcacttatcc tcaatcgcga ggccggttcg ttcttctttt
    16501 taggcgaatt gctggtcgat attccgctgc ccgtggatca accagtcgag gaaggatgcg
    16561 gcaaatgcgt ggcctgtatg acgatttgcc cgaccggtgc catcgtcgag ccatataccg
    16621 tcgatgctcg ccgctgtatc tcttatctca ccatcgaact tgaaggggcg ctcccggaag
    16681 agttgcgacc gttaatggga aaccgtattt acggttgcga tgactgccag cttatctgcc
    16741 cgtggaatcg ctattcgcaa ctcactacag aagacgattt cagcccgcgt aagccgctac
    16801 acgcaccgga actcattgag ttatttgcct ggagcgaaga gaagttttta aaagtcacgg
    16861 aaggatcggc gattcgccgt ataggtcacc tgcgctggct gcgtaatatc gccgtggcat
    16921 taggcaatgc cccttgggat gaaacgattt tgacagcgct ggaaagtcgt aaaggtgagc
    16981 acccacttct cgatgagcac atagcgtggg cgattgcgca gcaaatagag agacgaaatg
    17041 cgtgcatagt cgaagtgcaa ttgccgaaaa aacagcgtct ggttcgggtg attgaaaaag
    17101 ggttaccgcg tgacgcctga ttcatccaca gcttgtgaat aaaaataaaa acgcattgca
    17161 attcaagac
//
LOCUS       DAZXUK010000065        17092 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZXUK010000065 DAZXUK010000000
VERSION     DAZXUK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17092
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.065"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            86..1225
                     /gene="arnB"
                     /locus_tag="ACH3L8_001721"
     CDS             86..1225
                     /gene="arnB"
                     /locus_tag="ACH3L8_001721"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFT4084874.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1229..2197
                     /gene="arnC"
                     /locus_tag="ACH3L8_001722"
     CDS             1229..2197
                     /gene="arnC"
                     /locus_tag="ACH3L8_001722"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFT4084875.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2197..4178
                     /gene="arnA"
                     /locus_tag="ACH3L8_001723"
                     /pseudo
     CDS             2197..4178
                     /gene="arnA"
                     /locus_tag="ACH3L8_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            4175..5065
                     /gene="arnD"
                     /locus_tag="ACH3L8_001724"
     CDS             4175..5065
                     /gene="arnD"
                     /locus_tag="ACH3L8_001724"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFT4084876.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5065..6717
                     /gene="arnT"
                     /locus_tag="ACH3L8_001725"
     CDS             5065..6717
                     /gene="arnT"
                     /locus_tag="ACH3L8_001725"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFT4084877.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            6714..7049
                     /gene="arnE"
                     /locus_tag="ACH3L8_001726"
     CDS             6714..7049
                     /gene="arnE"
                     /locus_tag="ACH3L8_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFT4084878.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7049..7435
                     /gene="arnF"
                     /locus_tag="ACH3L8_001727"
     CDS             7049..7435
                     /gene="arnF"
                     /locus_tag="ACH3L8_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFT4084879.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7429..7701)
                     /gene="pmrD"
                     /locus_tag="ACH3L8_001728"
     CDS             complement(7429..7701)
                     /gene="pmrD"
                     /locus_tag="ACH3L8_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFT4084880.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7806..9161)
                     /gene="menE"
                     /locus_tag="ACH3L8_001729"
     CDS             complement(7806..9161)
                     /gene="menE"
                     /locus_tag="ACH3L8_001729"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFT4084881.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9158..10120)
                     /gene="menC"
                     /locus_tag="ACH3L8_001730"
     CDS             complement(9158..10120)
                     /gene="menC"
                     /locus_tag="ACH3L8_001730"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFT4084882.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(10120..10977)
                     /gene="menB"
                     /locus_tag="ACH3L8_001731"
     CDS             complement(10120..10977)
                     /gene="menB"
                     /locus_tag="ACH3L8_001731"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFT4084883.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10992..11750)
                     /gene="menH"
                     /locus_tag="ACH3L8_001732"
     CDS             complement(10992..11750)
                     /gene="menH"
                     /locus_tag="ACH3L8_001732"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFT4084884.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11747..13417)
                     /gene="menD"
                     /locus_tag="ACH3L8_001733"
     CDS             complement(11747..13417)
                     /gene="menD"
                     /locus_tag="ACH3L8_001733"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFT4084885.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13506..14801)
                     /gene="menF"
                     /locus_tag="ACH3L8_001734"
     CDS             complement(13506..14801)
                     /gene="menF"
                     /locus_tag="ACH3L8_001734"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFT4084886.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14880..15185)
                     /gene="elaB"
                     /locus_tag="ACH3L8_001735"
     CDS             complement(14880..15185)
                     /gene="elaB"
                     /locus_tag="ACH3L8_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFT4084887.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15240..15701)
                     /locus_tag="ACH3L8_001736"
     CDS             complement(15240..15701)
                     /locus_tag="ACH3L8_001736"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFT4084888.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15766..16683
                     /gene="rbn"
                     /locus_tag="ACH3L8_001737"
     CDS             15766..16683
                     /gene="rbn"
                     /locus_tag="ACH3L8_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFT4084889.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16874..17062
                     /locus_tag="ACH3L8_001738"
                     /pseudo
     CDS             16874..17062
                     /locus_tag="ACH3L8_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         3982 a         4627 c         4345 g         4138 t
ORIGIN      
        1 aatttcgctc caaatggcaa aatatacaca acactcttta tagcaaatat aagtggacag
       61 gtattcaatg gcggaaggaa aagcaatgtc agaatttttg cctttttcgc gaccagcaat
      121 gggcgtggag gaactcgctg cagttaaaga ggttctcgaa tccggttgga tcacaaccgg
      181 tccgaagaat caggcgcttg agcaagcctt ttgccagttg acgggaaatc agcatgccat
      241 cgcggtcagt tcagccaccg ccggaatgca tatcacgcta atggcgttgg aaattggcaa
      301 gggcgatgaa gtgattacgc cttccctgac ctgggtttca accctcaata tgatttcctt
      361 gctgggtgca acgccggtaa tggtggatgt cgaccgcgat acgctgatgg tcacgcctga
      421 agcgatcgaa tcagccatta cgccacgcac taaagccatc attccggtgc attatgccgg
      481 tgcgccagca gatattgacg ccattcgcgc cattggcgaa cgttacggca tcgcagttat
      541 cgaagatgct gcccatgccg tcggtacgta ttacaaaggg cgacatattg gcgcaaaagg
      601 taccgctatt ttttcatttc atgccattaa aaatattacc tgtgctgaag gtggcctgat
      661 tgtaactgat aatgaaaacc ttgcccgcca gctacggatg ctgaaatttc acggtctggg
      721 tgtcgatgcc tatgacagac aaacctgggg ccgtgcaccg caggctgaag tcttaacacc
      781 gggctataag tacaatctga ccgatattaa cgccgcgatt gccctgacac agttagtcaa
      841 attagagcac ctcaacaccc gtcggcgcga aattgcccag caatatcagc aagcactggc
      901 agctctcccc tttcagccat taagccttcc cgcctggccg cacgttcacg cctggcatct
      961 gtttattatt cgtgtcgatg aacaacgttg tggtatcagt cgcgatgcgt tgatggaagc
     1021 gttaaaagaa agaggcattg gtaccgggtt acatttccgc gccgctcaca cacaaaaata
     1081 ttatcgcgag cgttttccca cgctgtcgtt accgaatacc gaatggaata gcgaacgcat
     1141 ctgttcgttg ccgctgttcc cggatatgac taccgccgat gccgaccgcg tcatcacagc
     1201 ccttcagcaa ctcgcaggac aataagccat gtttgaaatc caccctgtta agaaagtctc
     1261 ggtggttatt cccgtttata acgagcagga aagcttaccg gaattaatca ggcgcaccac
     1321 cacagcctgt gaatcgttgg ggaaagagta tgagatcctg ctgattgatg acggcagtag
     1381 cgataattcc gcgcatatgc tggtcgaagc ctcacaagcg gagaacagcc atattgtgtc
     1441 tattttgatt aaccgcaatt acgggcaaca ttcagcgatt atggcgggat tcagtcacgt
     1501 tactggcgac ttaattatta cccttgatgc cgatctccag aatccgccag aagaaatccc
     1561 ccgcctggtg gcaaaagccg atgaaggtta cgacgtggta gggactgtac gccagaaccg
     1621 ccaggacagc tggtttcgta aaacagcttc gaagatgatt aaccggctta ttcagcgcac
     1681 cactggcaaa gcgatgggtg actacggttg tatgctgcgc gcctatcgcc gtcatattgt
     1741 cgatgcgatg ttgcactgcc atgaacgcag cacctttatc ccgattctgg cgaatatctt
     1801 cgcccgccgt gcaattgaaa ttccagtaca tcatgccgag cgtgagtttg gtgaatccaa
     1861 atacagtttt atgcgcctga ttaatttgat gtacgacctg gtgacctgcc ttaccacaac
     1921 gccgctacgt atgctgagtc tgctcggcag cattattgcg attggaggtt ttagcattgc
     1981 ggtgctgctg gtgattttac gcctgacctt cggaccacaa tgggcggcag aaggcgtctt
     2041 tatgctattt gccgtgctgt ttacttttat tggcgctcag tttatcggca tgggattact
     2101 cggtgaatat atcggcagga tctacaccga tgtccgcgcc cgcccccgct attttgttca
     2161 gcaagttatc cgtccatcca gcaaggaaaa tgaataatga aaaccgtcgt ttttgcctac
     2221 cacgatatgg gatgcctcgg tattgaagct ctgctggctg ccggttacga aattagcgcc
     2281 atttttaccc ataccgataa tcccggtgaa aaagcctttt atggttcggt ggctcatctg
     2341 gcggcggaaa gagacattcc ggtttatgcg ccggataacg ttaatcatcc gctgtgggtg
     2401 gaacgcattg cccaactgtc accagaggtg attttctctt tttattatcg ccatcttatt
     2461 tgcgacgaga tttttcagct cgctcccgca ggtgcattta atctgcacgg ttcgctgtta
     2521 ccaaaatatc gtggtcgcgc gccgctgaac tgggtgctgg tgaacggtga aacggaaacg
     2581 ggcgttacat tgcaccgaat ggtaaacgtg ccgatgctgg ggccattgtg gcgcaactgc
     2641 gcgttgccat tgcgccagac gatatcgcta ttacgctgca tcataaattg tgccatgccg
     2701 cgcgccagct actggaacag acattacccg ccattaaaca cggtaatatt ctggaaatcg
     2761 cccagcgcga aaacgaagcc acctgttttg gtcgcagaac gccggatgac agtttccttg
     2821 aatggcataa accggcatcc gtactgcaca acatggtacg tgccgttgcc gatccgtggc
     2881 cgggtgcctt cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc
     2941 ctcatgccag caaagcacag ccggggagcg tgatttctgt tgcgccactg ctgattgcct
     3001 gtggcgatgg cgcgctggaa atcgtcaccg gacaggcggg cgacggcatt actatgcagg
     3061 gctcgcaatt agcgcagatg ctgggcctgg tgcaaggttc acgcttgaat agccagcctg
     3121 cctgcaccgc ccgacgccgt acccgggtac tcatcctcgg ggtgaatggc tttattggca
     3181 accatctgac agaacgcctg ctgcgcgaag atcattatga agtttacggt ctggatattg
     3241 gcagcgatgc gataagccgt tttctgaatc atccacattt tcactttgtc gaaggcgata
     3301 tcagtattca ttccgaatgg attgagtatc acgtcaaaaa atgtgatgtc gtcttgccgc
     3361 tggtggcgat agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg
     3421 attttgaaga gaatctgcgc attatccgct actgcgttaa gtaccgtaag cgaatcatct
     3481 tcccgtcaac ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggaccatt
     3541 ctaatttaat cgtcggcccg gtgaataaac cacgctggat ttattcggtg tcaaaacaat
     3601 tacttgatcg ggtgatctgg gcctatggcg aaaaagaggg tttacagttc accctcttcc
     3661 tcccgtttaa ctggatggga ccacgactgg ataaccttaa tgcagcgcga attggcagct
     3721 cccgcgctat tacgcaactc attctcaatc tggtagaagg ttcaccaatt aagctaattg
     3781 atggcggaaa acaaaaacgc tgctttactg atatccggga cggtatcgag gcgttgtacc
     3841 acattatcga aaatgcggga aatcgctgcg acggcgaaat tatcaacatt ggcaatcctg
     3901 agaacgaagc gagcattgag gaactgggcg agatgctgct ggcgagcttc gaaaaacatc
     3961 cgctgcgcca tcatttccca ccgtttgcgg gctttcgcgt tgtcgaaagt agctgctact
     4021 acggcaaagg atatcaggac gtagagcatc gtaaaccgag catccgcaat gcccaccgct
     4081 gcctggactg ggagccgaaa attgatatgc aggaaaccat cgacgaaacg ctggatttct
     4141 tcctgcgcac cgttgatctt acggataaac catcatgacc aaagtaggct tacgcattga
     4201 tgtcgatgcc tttcgtggca cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa
     4261 gcataatatt caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct
     4321 ctggcgactg gtgaagccac agtttttgtg gaagatgctg cgctcaaacg cggcatcgct
     4381 ttatggctgg gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc
     4441 caatgccgat atcattcgtg aagcggctaa acatcacgaa gtcggtctgc acgcctggga
     4501 tcaccatgcc tggcaagccc gtagcggtaa ctgggatcgg caaacaatga tcgacgatat
     4561 tgcacgcggt cttcgcactc tggaagagat tatcggtcaa ccggtaacct gttctgccgc
     4621 tgcgggctgg cgtgccgacc agaaggtgat cgaagcaaaa gaagcgttcc atttgcgcta
     4681 caacagcgat tgtcgtgggg ccataccgtt ccgtccactg cttgaatcag gaaaccctgg
     4741 cactgcgcaa attccggtga ccttacccac ctgggatgaa gtgattggtc gggatgtgaa
     4801 agcagaagat tttaacggtt ggttactcaa ccgcatcctg cgagataaag gcacgccggt
     4861 ttataccatt catgcagaag ttgaaggctg cgcttatcag cataattttg tggatctcct
     4921 caaacgcgca gctcaggaag gcgtgacatt ttgcccttta agcgaactgt tatcagagac
     4981 gttgccgctc ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg
     5041 ccaacaaatt gcgggtagtc gctgatgaaa tcggtacgtt accttatcgg catcttcgcg
     5101 tttattgcct gctattacct gttaccgatc agcacgcgtc tgctctggca acccgatgaa
     5161 acgcgttatg cggaaatcag tcgagaaatg ctggcatccg gcgactggat tgtgccccat
     5221 ctgttagggc tacgttattt cgaaaaaccc attgccggat actggattaa cagcattggg
     5281 caatggctat ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg
     5341 ttaactgccg cgctggtgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct
     5401 ctactcgcca cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc
     5461 gtgctcgatc cgtttattgc attctggctg gtggcgggaa tgtgcagctt ctggctggca
     5521 atgcaggcac agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc
     5581 ggcatggggg tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg
     5641 ccatgggtag cgacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtt
     5701 atcagttgcg tactgacggt tctcccctgg ggactggcga tagcgcagcg ggagcctgac
     5761 ttctggcact attttttctg ggttgagcat attcaacgct ttgcactaga tgatgcccaa
     5821 catagagctc cgttctggta ctacgtgccg gtcatcattg ccggtagcct gccgtggctg
     5881 ggattactcc ccggtgcact gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc
     5941 tatttgttga gctggacgat aatgccgctg ctgtttttct ccgtcgctaa aggcaaattg
     6001 cccacctata ttctttcttg ctttgcacct ctggcaatgc tgttggcgca ttacgcgttg
     6061 ctggcagcaa aaaataatcc tctggcgctg cggattaatg gctggattaa catcgctttt
     6121 ggcgtcactg gcattattgc cacatttgtg atctccccct ggggaccaat gaacacgccg
     6181 gtgtggcaaa ccttcgagag ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg
     6241 gcatttttcg gctggtacac cttaacaaac gtcgaaaaga cctggtcttt cgccgcgctt
     6301 tgcccgctgg ggctggcgtt gctggtagga ttttcaattc ctgacagagt catggaagga
     6361 aaacatccgc aattttttgt cgagatgaca caagaatcac ttcagccaag ccgctatatt
     6421 cttactgata gcgtcggtgt tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc
     6481 atcatgtatc gccagacagg tgagttgaaa tacggcctta attatccgga tgcgaaaggg
     6541 agatttgtca gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt
     6601 attacactcg tgctttcggt tgaccgcgat gaagatatca acagtctcgc cattccgccc
     6661 gcagatgcca tcgttcgtca ggagcgtctg gtgctgattc agtatcgtcc caaatgatct
     6721 ggctaacatt agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg
     6781 caacctgctt tgtggcgata aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg
     6841 cgctggcttg tcttggtctt gccatgatgc tctggctgct ggtcttgcag aacgtaccgg
     6901 taggcattgc ctacccgatg ttaagtctga attttgtctg ggtgacgctg gctgcagtaa
     6961 aactgtggca cgaaccggta tcgccgcgtc actggtgtgg ggtggcgttc attattggtg
     7021 gcattgtgat cctcgggagt acggtgtaat gtgcctgata tggggattat tcagcgtcat
     7081 tattgcctca gttgcgcaat taagcctggg ttttgcggca agtcatctgc cgccgatgac
     7141 gcacttatgg gattttatcg cggctctgtt agcttttggc ctggatgccc ggatactgtt
     7201 actcgggctg ctgggttatc tgctttccgt tttttgttgg tataaaacgc tacataaact
     7261 tgcgctaagc aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc
     7321 aatggtactt cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg
     7381 tattatgagc gggttgatgc tgattttcct gcccacgaca aaacaacgtt actgagtttt
     7441 ccctgccgct gtacactgcc gttcccactc atcggggcta taactgcttg ccgagaggac
     7501 tttcaccgta tgcagctttt cacgattaat acaataaagc gcattctgca aaggcgagag
     7561 taaatccccc actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc
     7621 gccagcatcg cagagcataa gtacatgtct gttatcttgt ttattgcagc acgatttttt
     7681 gtgccagcca ttccattgca ttatcctgtt tgctaagagt tttcacatca atttacagat
     7741 taacagataa actctttatc aacggcaatg gaatggcaga aatgagaagt gaaacggagg
     7801 gttaattatt gctgacgttg cacccactct tttagcgcct gacgtgaaat tttaataccg
     7861 ccgtttttca gctccggcgg taaagtcagc cagcgcaccg gttgttgaaa acgagccagc
     7921 ttatctttca cccattcgcc aagatcaacg gtttgctggt catactccac caccgctacc
     7981 ggccgatgac caaactcctt gtcggcaacg gaaacgataa acacctgcaa aaccgcagga
     8041 tgtgcagcaa taacacgctc gacttcttcc ggctgaatac cctcaccgcc actgaagaac
     8101 agattgtcta aacgtccgac aatggtcagc ttgccattat gcatctcacc gcgatcgcgc
     8161 gtagcgtacc agccttcgtc attaaccagt gaaaccagtt gcccgttacg ccagtaacct
     8221 tctgccatac tggcagcccg cagccacact tcattattaa cgattttcac ttcccgaccc
     8281 ggcagcggca aaccaacgtc tgccaggccg tcggcttctt tcgcacatac cgtggaggca
     8341 aactcggtca gaccatagcc gcaaaagcaa cgaatcccct gctcgcgcgc ctgttccgtc
     8401 aactcgaccg ggatagccgc gccgccaagt aacaccgctt tcagggaaac ggaactgcgg
     8461 ttaaccagca aacgccagag ttgtgttggc accagtgaag cgtgagtaca gcctgccagc
     8521 atttgctcca atggctgttt atcacgcacc gtcatccgcg caccagcgta taaccagcgc
     8581 cacataattc cctgaccgga gacgtgaaac agcggtaaag agagcaacca gtcatcgtga
     8641 tcgccaaacg gaatcagcga taacacacct tgcgcactgg caagatgggc ctgataagta
     8701 tggacagcgg ctttcggcaa accggtagaa cccgaggtca acgtcattga gcacagacgc
     8761 gtcggctgcc acgcagcggc atgtacgcct tcaaccagct gaatgtgcag cgacgttaat
     8821 gccggaaacg tgttttcccc atccggcacc agcgcaaatt gcagcgtcag attgggcagc
     8881 aattcttcaa gcaacggttg cggcagctga gggttcacgg gcaacacccg cgccccgcat
     8941 tgcagtaacg ccagccaggc gagcagcgtt tgcggcgtat tccacgcccg caacatcacg
     9001 ccgctgccct caaccacccc ctgcgccgca aagcccgtag ctaactcatc gacgcgagca
     9061 caaagctcgc gccagttgag ttgctcgtca ttaagacgta aggcgatggc ttctccccgc
     9121 acttgccgcc agtgacgcca cggccagtca gagaagatca taacaactgc tcctgtacat
     9181 cagcgtccac taacggcagt gggctccccg gccagcgacg aacctgctgc gcctgcatca
     9241 gatccagcgt gtccagtccc ggaatggtgt ctggcgttaa ccaggcagca atccgcgcca
     9301 gttgcgttag acctaagctc gactcaatgg aagaactgat caccgccgtc agccccagcg
     9361 catgcgccgc ctgtacctgc tcgcgtactt tttccagact gcccgtgagc gtgggtttga
     9421 taactaccgc gcgcacgccc tcttcagcca caaaggcaaa atccggctcg cgcaggcttt
     9481 catcccaggc aatggcaatg ccggtttcac gggcaaacgc tcgcgaatca tcgcgggttt
     9541 tgcacggctc ttcgagaaac gcgatgcggt ggcgataatc cgggttaaca tatttggcaa
     9601 actgctgacc tttcagcggt gtccaggcgc gatttgcgtc aagacgcaaa tgcagatccg
     9661 gaattgcctc cagcaacaga ttcaccacca tgccgtcgcg caccgcttcg tacaatccca
     9721 ctttgacctt cgccactttc tcgcccggca tatcggcaag tttgaggatc agatcgtccg
     9781 gatcgccatt acacagcggt gccgcacggt agttggctgc ttgcggcaac gtatctgtca
     9841 gttctgccaa tgcacagctt acgccaaagg ccacggaagg catctgcggt agctcgcaat
     9901 cgcctgccag ccagttattt acccaggcaa gcagcacgct ttgcgcctct tcccagtttt
     9961 cctggctgaa gcccggcagt ggggagatct ccccccaccc ttcgcgctcg ccctcacgca
    10021 ggcaaacgta cagcccgtcg cgggttttta accgcctgtc gcgcagaacc acccccgcgt
    10081 ccatggggat ctgccagcgg tatacctgcg cgctacgcat tacggattcc gtttaaattt
    10141 gctgaaatca ggctgacgtt tctggttgaa agcgttgcga ccttcctgac cttcttccgt
    10201 catgtagaac agcatggtgg cgttgcccgc cagctcctgc agccccgcct gcccgtcaca
    10261 gtcggcgttc agcgcagctt tcaggcagcg cagcgccatc gggctgtttt gcagcatttc
    10321 gcggcaccaa cggacggttt ctttttccag atccgccagc ggtaccacgg tgttgacaag
    10381 gcccatatcc agcgcctgtt ttgcgtcgta ctgacggcac aggaaccaga tttctcgcgc
    10441 ttttttctgc ccgacgatac gagccatgta ggaagcgccc cagccgccgt cgaaggaacc
    10501 gactttcggg ccagtctgac cgaagatggc attatctgct gcgatagtca ggtcgcacat
    10561 catgtgcaga acgtgaccac cgccgatgga gtagccagcc accatcgcga caaccggttt
    10621 cggacaggta cggatctgac gctggaagtc cagcacgttc aggtgatgta cgccggaatc
    10681 atctttatag ccgccgtaat caccacgcac tttctggtca ccaccggagc agaacgcttt
    10741 atcgcctgcg ccagtcagaa tgatcacgcc gatattgtcg tcataacgcg catctgccag
    10801 tgcctggatc atctctttta ccgtcagagg acggaaggca ttgcgcacct gcggacgatt
    10861 aatggtgatt tttgcgatac cgtcggtgga tttttcataa cgaatgtcct cgaaaccttc
    10921 ggagcagtcg tgccattcaa ccggtgcgta aggcattgct tcatcaggat aaatcatatt
    10981 gtgtccttta gtcagaaacg caagatctgc gccagacttg cgattacgcc agcgggattt
    11041 tcccgatgcg cgttatgtcc ggcgcgagga atgacatggc agtcggcagc cagttccgcc
    11101 gccagggcgc ggaatttgct gtcacgttca ccacataaat aataaaacgc aaatgtgcgg
    11161 gcgctaaggt tagcacgtaa atcaggctgg acggcgagag aagtcgcctc cagcatggcg
    11221 gcaagggttg cgccattatt gttgctgcgc agcgccacca gctcccggcg ttgatcgtca
    11281 ttgagcgagg caaaaacagg ctgttgatac cagtcggcaa ataccgccgt taacggttct
    11341 gtgcgaaaac gctgcgccca ttggcgatcg gaacgctgac gttccgcacg ttgttcagca
    11401 ttttgcagcc ccggatgccc gccttcgaca ataaccccac aaagccccgc cagcccctgg
    11461 caagccgcca tcatcgccac ccgtccgcca agcgagtacc ccaccagcca gaagtcaagg
    11521 atgttgtaac taaccaaggt tttacgcagt aagtcggtga catcatcaaa tccatcgacg
    11581 ctaatcgccg ccgaaccacc atgacctagg agatcaacat acaaccgtga gtagtcggca
    11641 aacgcctcgc ccacttcttg ccattcgtgg caatcgccgg aaaaaccgtg caaaaacacc
    11701 agccagggta aacctagttt tccgtgtttt gcctgcgcgt gcaggatcat aaatggctta
    11761 cctgcgccag aagttgctgg agcgtttgcg caccatcggt gtcgttaacc accatttcaa
    11821 tcaccgtggt ggttggcgtg cgccaggcgt cggcaaatgc cgtttcaagt tcctgccagt
    11881 tttgcggatg atgatatttc agctcgaaca tcgcggcggc gtgctcaaaa tggacgtttt
    11941 gcggcatcag atagaaacgc tcacgctcgc tttgcggcgt tggcaacagc gagaaaattt
    12001 gcccgccgtt gttgttcacc acaattaata ccagcggcgc agaaacctga cgcaataacg
    12061 ccagcgcgtt gagatcgtaa agcgcggaga gatcgcccac aatcgccagc gtcggtttgc
    12121 cgcttgcccg ctgaacgcct gcggcggtcg aaagcagtcc gtcgataccg ctggcccctc
    12181 ggttgctgta caccgggtaa cctgccggaa gttgcgaaag cgcatcaatc agacgtacca
    12241 ccaggctgtt accaacaaac aattgcccct gttcaggcag atagtcgctg atgcgatgcg
    12301 ccagttgcgc ttcgccaaac gcatcacggc gggcaataac cgcctgcatt gcctgttccg
    12361 ccaggcgcgg gatttcaacg caccagggct ggcgtttttc tgccggatgc agctccagcc
    12421 agtcggcaat attggcaatt aagcgacgtc cgcgatggtg tgccggatca agtcgccctt
    12481 caatgtcatc aacaatccag tactcttctg gttcacagct tgcctgccat tgcaggagcc
    12541 gtttccccgt caggctactt cccagttgca ccacaatttg cgcctgctgc agctcgctgg
    12601 tcgctttggc attgcctaac caaagatcgg cacacggcag cggctgcccg gtttgtgaca
    12661 gcacatcgcc aatcagcggc cagccaagag tttgcgccca cagggcaact tttttgccct
    12721 cttccgcact catgcgcccg gcaaccacca cgccgcgctt ttgtcgccag aagaaccagt
    12781 cgcgctgttt ttcactttcc agacgaggcg cttcacgcag ccacggtttg tcgtcctgcc
    12841 accagtcacc cagacgctgt tgccagctaa gcccggtatc gtccatttcg ccatacagcg
    12901 gttcagcaaa cgggcagttg atatggactc cccccgcatg aagcgtaccg agagcgtggt
    12961 cgatggtaga aaccagccaa cgtgcgggga tatcctgggt cgggcgcggc aatgaaatac
    13021 tgtgcgtggg gtgagaggcg aacattcccg gctggcgaat tgcctgattc gcgccgcagt
    13081 caattagctc cggcgggcga tcggcggtta agagaatcag tttttctccg gttaacccgg
    13141 cttcaatcag tgccggatag agatttgcca ccgccgtgcc ggaggtcaca atcaccgcca
    13201 ccggctgctt gctgactttc gccagcccca gcgccagatg ccccaaccca cgctcatcga
    13261 aatgggtgtg gtgaatgaat gcggaattct ccgccgccgc taacgttaac ggtgtagaac
    13321 gcgagcctgg ggcgatacag atgtgtctga cgccgtgacg cgttaatgct tccagaatga
    13381 ccgccgccca gcgtcggtta aatgcgctta ctgacatgag tttgtccggt atcaatattg
    13441 cggctaagta taaggagcta caaaaataga ttattgatat gaatcggtaa tgatgcgact
    13501 cattactatt ccatttgtaa taaagtacgc agccctgccg ctttgttgtc gatttcctgc
    13561 cactcttgct cggggtcgga accacggaca atgcccgcgc cagcatataa tcgcacgaca
    13621 ttgccgctaa tttttgctga gcgcagggaa acgcagaatt cgctttgttg taatgagaga
    13681 tagcccgcag aaccggcgta ccattctcgg gtgaacggtt cgtgacgggc gataaactgt
    13741 cgcgccagat cgcgcggtaa gccagcaact gctgccgttg gctgcaactg atgtaaacag
    13801 atcacatcat ccgctttgtt gagtgaagtc cagatacagc ggcgaagatg ctgcacttta
    13861 cgcagacgca gtacctgcgg cggtaaaaca tccagcgtct gggtatcggc ctgtaatcgt
    13921 tggcagatat cttccaccac cagcatgttc tcgcgctggt ttttatcatc cgccatcagc
    13981 cactctccta actgctgcgc ctgcttatca tcaggatgat ttgctactgt tcccgccagc
    14041 gcttcagtac gcagcgcttt gtcacgccgc cgccataacc gttccggtga agagccaaga
    14101 aaagcatttt cgccatcaaa ggccatgtaa aaatggtagc aattcagatt cagtcgacga
    14161 ctggcagcca tcatcgccgc cgcgttgacc ggacttgcga aatgcaggtc agttgcccga
    14221 gcgagcacca ctttgtcgag ctcaccttcg gcgatggttt tcgttgccag ttcgattaat
    14281 tgcgtccagc ccgttttgtc cggccagtgt tgttctcgcg tggtggttaa atgtaaccca
    14341 ggcaagggct tgatactcac cagtgtggcg ataaattctt ttgcctgaat cgcatcgtgc
    14401 tgaagggagc tttcgctgaa tagcgtcagc cgcagcgtgg ctttaccgcc acagcgtcgc
    14461 cattccaggc ggggtaaaag taaattgccc tgcgacgggt caaaagcatt cagcccccaa
    14521 atgcgtaagt cagcgtgttc cgggtgctgg cgaagaaaac gttgtgcctg gtccaacgac
    14581 gtaaaacggg taatcgcgcc caggacggca gcttcttcat caccattacg ttgttgccag
    14641 tagaattgcg ggtacgtttg ctgactggcc agccagctca aggcatcaaa agcgtctttg
    14701 agagggaaag gaatatcgat aacccgaatg ccgggtgtcg ccggaatctc ttgcgacaaa
    14761 tggcgcagta gattttccag cgccgtagta agtgattgca cgcggacctc tccctgttaa
    14821 aagcctcaca ttatacgggg tactacaaaa aaaatgcagt accccggtgt agggaggttt
    14881 taacggcgtg ccagcaacag tcctagtacc agcccgacgg ccgcgcccac accaattcct
    14941 tgccagggtt tttcgtggac gtagtcatca gcacgataaa cagcctgctt cgcccgatag
    15001 taataactgt ctgaagcctg gctaacccgt tttttcacat catccagcgc tttttctgca
    15061 tgcgctttca gctcaacata tttctgatcg gcgggatcgc cagaggagcg gagcacctct
    15121 tccagtgttt cactaagcag cgtcaggtcg tcatcgatac gtgtatcacc aaactgatta
    15181 gacattctcg ttctccattt gcgtaaaacc tgtcactaac tatagacaag agttgttgat
    15241 tacgcctgaa ttacctcgcg tgccatccca atgtgtggga taccatcttc ttcatagacc
    15301 tccgtcacag ggataaaacc gaaactctgg tagaagtttt gcagatgcgc ctgcgccccg
    15361 aggtaaacag gcttatcagg ccagtgatgc gtacatgttt cgagtgtttt actcatcagc
    15421 tgctgcccca ctttttcacc acgcaacgct tcactgacaa tcacccgacc tataacgacc
    15481 ggctcaagat catcatcact tttcagaatc ctcgcatacg ccaccagttc atcatttttc
    15541 caaccgagaa tatggcgatt atcccccgtc aaatcatcac catcgatatc ctgataaggg
    15601 caattctgct cgacaacaaa caccgcgcaa cgcagttgta ataaggcata aagctgggaa
    15661 accgataatt cagagtgatg cagatcttgc cattcaatca taatgctctc cttttagatg
    15721 tatgccttat actagccgga atattttttg aaacgtgatg aactcatgga attaattttt
    15781 ttaggtactt cagccggtgt gccaacccgc acgcgcaatg tcacggcaat attgcttaac
    15841 ctgcaacatc cgacccagag cggactttgg ttgtttgact gcggtgaagg cacccagcat
    15901 cagctactgc ataccgcctt taaccctgga aaactggaca agatttttat cagtcacctt
    15961 catggcgatc atctttttgg tttacccggc ttgctgtgca gtcgttctat gtcaggcatt
    16021 atccaaccct taacgattta tggtccgcaa ggtatccgtg aatttgtgga aaccgcgctg
    16081 cggattagcg gctcatggac cgattatccg ctggaaattg tcgaaattgg cgctggcgaa
    16141 attctcgatg atggcctgcg caaagtaacc gcttatccgc tggaacaccc actggaatgt
    16201 tatggctatc gtattgaaga acatgataaa ccgggtgcat taaatgccca ggcattaaaa
    16261 gctgctggcg tgccgcctgg cccactgttt caggaattaa aagcgggcaa aacaatcacg
    16321 ctggaagatg gaaggcagat taacggcgca gattacttag ctgctccagt gccaggtaaa
    16381 gcgctcgcta ttttcggcga taccggcccc tgcgatgccg cacttgacct ggctaaaggt
    16441 gtcgatgtca tggtgcacga agcgacgctg gatataacca tggaagccaa agccaatagt
    16501 cgcggccata gctctacacg ccaggctgcg acactagccc gtgaggctgg agtcggcaag
    16561 ctaatcatta cccacgtcag ctcgcgctat gatgacaaag gttgtcaaca cctgttacgt
    16621 gaatgcaggt caattttccc ggcgactgaa ctggcgaatg atttcaccgt gtttaacgtt
    16681 taactctgct tattattaaa aacagggtga aacttgccct gttatagcaa cccgcgctac
    16741 atatatccac ccacacaggc atattgagcg atttttgata tttattcatc tccttacgca
    16801 ctatattcag agcaataagt tgcctctttt catcttgatt cagcacaacc tttgcaacta
    16861 aaaggagccc atgatggtta cagttgtcag caattattgt caattatctc aaacccaact
    16921 cagtcagaca tttgcagaaa aatttactgt gaccgaggaa ttactgcagt ctttaaaaaa
    16981 aacagcgtta tccggagatg aagaaagtat tgagttactg cataatattg cgttaggtta
    17041 tgatgaattt ggaaaaaaag ctgggtaatg ctgccaactt actgatttag tg
//
LOCUS       DAZXUK010000066        16822 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZXUK010000066 DAZXUK010000000
VERSION     DAZXUK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16822
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.066"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(240..581)
                     /locus_tag="ACH3L8_001739"
     CDS             complement(240..581)
                     /locus_tag="ACH3L8_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFT4084890.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(594..1466)
                     /gene="copD"
                     /locus_tag="ACH3L8_001740"
                     /pseudo
     CDS             complement(594..1466)
                     /gene="copD"
                     /locus_tag="ACH3L8_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(1470..1844)
                     /gene="yobA"
                     /locus_tag="ACH3L8_001741"
     CDS             complement(1470..1844)
                     /gene="yobA"
                     /locus_tag="ACH3L8_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFT4084891.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            1983..2213
                     /gene="holE"
                     /locus_tag="ACH3L8_001742"
     CDS             1983..2213
                     /gene="holE"
                     /locus_tag="ACH3L8_001742"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFT4084892.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            2315..2971
                     /gene="yobB"
                     /locus_tag="ACH3L8_001743"
                     /pseudo
     CDS             2315..2971
                     /gene="yobB"
                     /locus_tag="ACH3L8_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
     gene            2995..3657
                     /gene="exoX"
                     /locus_tag="ACH3L8_001744"
     CDS             2995..3657
                     /gene="exoX"
                     /locus_tag="ACH3L8_001744"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFT4084893.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(3654..5714)
                     /gene="ptrB"
                     /locus_tag="ACH3L8_001745"
     CDS             complement(3654..5714)
                     /gene="ptrB"
                     /locus_tag="ACH3L8_001745"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFT4084894.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(5924..6583)
                     /locus_tag="ACH3L8_001746"
     CDS             complement(5924..6583)
                     /locus_tag="ACH3L8_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFT4084895.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(6910..7266)
                     /gene="yebF"
                     /locus_tag="ACH3L8_001747"
     CDS             complement(6910..7266)
                     /gene="yebF"
                     /locus_tag="ACH3L8_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFT4084896.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            complement(7333..7623)
                     /gene="yebG"
                     /locus_tag="ACH3L8_001748"
                     /pseudo
     CDS             complement(7333..7623)
                     /gene="yebG"
                     /locus_tag="ACH3L8_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            7757..8935
                     /gene="purT"
                     /locus_tag="ACH3L8_001749"
     CDS             7757..8935
                     /gene="purT"
                     /locus_tag="ACH3L8_001749"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFT4084897.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKSDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(8991..9632)
                     /gene="kdgA"
                     /locus_tag="ACH3L8_001750"
                     /gene_synonym="eda"
     CDS             complement(8991..9632)
                     /gene="kdgA"
                     /locus_tag="ACH3L8_001750"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFT4084898.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(9669..11480)
                     /gene="edd"
                     /locus_tag="ACH3L8_001751"
     CDS             complement(9669..11480)
                     /gene="edd"
                     /locus_tag="ACH3L8_001751"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFT4084899.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(11715..13190)
                     /gene="zwf"
                     /locus_tag="ACH3L8_001752"
     CDS             complement(11715..13190)
                     /gene="zwf"
                     /locus_tag="ACH3L8_001752"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFT4084900.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            13528..14397
                     /gene="hexR"
                     /locus_tag="ACH3L8_001753"
                     /gene_synonym="yebK"
     CDS             13528..14397
                     /gene="hexR"
                     /locus_tag="ACH3L8_001753"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFT4084901.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14525..15967
                     /gene="pyk"
                     /locus_tag="ACH3L8_001754"
     CDS             14525..15967
                     /gene="pyk"
                     /locus_tag="ACH3L8_001754"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFT4084902.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(16098..>16822)
                     /gene="lpxM"
                     /locus_tag="ACH3L8_001755"
                     /gene_synonym="msbB"
     CDS             complement(16098..>16822)
                     /gene="lpxM"
                     /locus_tag="ACH3L8_001755"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFT4084903.1"
                     /translation="AIVDEMFATAPQAMAMMAELAIRGPEKIQPRVGWQGLEIIEEMR
                     RNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAMFHNQGNPVFDYVWNTVRRRFGGRLH
                     ARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPAIGRLMKVCRAR
                     VVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHTIERRMNEEVEIFVGPRPEQYTWILK
                     LLKTRKPGEIQPYKRKDLYPIK"
BASE COUNT         4114 a         4362 c         4148 g         4198 t
ORIGIN      
        1 ctgtattcgg tccagggaaa tggctcttgg gagagagccg tgcgctaaaa gttggcatta
       61 atgcaggctt agttgccttg ccctttaaga atagatgacg acgccaggtt ttccagtttg
      121 cgtgcaaaat ggtcaataaa aagcgcggtg ctcatcagct taaatgttaa aaaccgcccg
      181 ttctggtgaa agaactgagg cggttttttt attggaaatc aaaaggctat tttaggtaat
      241 taacagagtt tttcagctcg ttctataaac ggtgccagac tcattttttc gccgggattg
      301 ttaggatcat caatctgaat caccgaaatg ggttgggcat tggtcttccc actggcaact
      361 tccttttgtg cgatatcgtt taaaggatac tgcacgaggg tacttgggtt gatgacatac
      421 aaagcattac ccggtcggca agtcagcatt acctcttcgc gattaaacgc ccatttgtct
      481 ttacccactt caaaacgact cacggttatc acctgtggag ccgccagcgc cgctgcagaa
      541 ctggtgagta acagaaacgc cagaatactt tttttcatca ttttcgtaat ccatcaaaaa
      601 ggttcgagag tcgcaagcag gctgatgatc accagcacta ccgcacctat cgcccattcg
      661 agtttagtca tccacacaaa ccagggtgcc gcacgatctt catcctgcct catgcgcggt
      721 acaagaacat aacgattagc cagcgcaatg accaccataa tcatcaccag gatggctttg
      781 agcaataaga gttggcccca gtaagtcgta agcgtggggg gaaatccggt aatcaacaat
      841 gcattaagca cgccgcttgc cagtacgccg atcaccgcaa aatgcccgca ccaggaaaaa
      901 cgcatcagcg cctgaatagc ctagtgtcgc caacggcctt tgatgagttg catacaccag
      961 agcaccggca gtaatccccc aaaccacgcc gccgcacaaa tcaggtgaat cgcgtgatta
     1021 gtctgatgga ttttcgctgt taccccttca ttcagcgtcg cataccccac tcccgccagc
     1081 agaataaatt gcgcggtagt gagcataaac agcaaacgtg gcatattccg cggttgcata
     1141 agggcgacga tcaacgttac gagggcgaga acaatttgcc ataaccagac gccaccaaac
     1201 tgcgtttgca aaacagcctg ccaaatgttt ggcgaaaata catctgtcca tcccgttccc
     1261 atcaacccgc cctgaacggc gagcattgca gtggcactga tcaaactcca gacggcagcg
     1321 tgctgttgta aacgcagaga acgcttcgtc agcaagcgac gaatcattaa gggggccagc
     1381 caggcaccat acattgcaaa gccaaagacc agcatcaacg aggtgaaatg gataaatcgc
     1441 agcgcgatcc aggtaaacgc cagcatgatt tatttcacgc taaaggtgta gtgtcctttc
     1501 gttttatgcc cgtccaccga aacaacatgc cagtcaacgg tgtaggttcc gggtttcagc
     1561 gaatcggcca gtggaacaat cagttgcttt tggtcctgct cattccgctt cgctggtaat
     1621 gttttaatat tttcgttttt tggccccgtg atttttgcac cactgaatcc tgtttcaacg
     1681 ccttccgaga agtttaaagt gattgcctgc ggggcagctg tcacttgcgc gtttgccgca
     1741 ggatactgat gcgttaaatg cgcatgtgcc cagacggaag gtgtcaccaa cgaagtggtc
     1801 aggattgcca gcgcgtagcg aagggagcgt gcagttgaag ccatattatc tattcctttt
     1861 tgtaataact tttttacaga gcataacctt gtctaatgtc tgagtcgagg atcatcaatt
     1921 ccggcttgcc atccaggctc actcttagta acttttgccc gcgaatgatg aggagattaa
     1981 gaatgctgaa gaatctggct aaactggatc aaacagaaat ggataaagtg aatgtcgatt
     2041 tggcggcggc cggggtggct tttaaagaac gctacaatat gccggtgatc gctgaagcgg
     2101 ttgaacgtga acagccagaa catttgcgca gctggtttcg cgagcggctt attgcccacc
     2161 gtttggcttc ggtcaatctg tcacgtttac cttacgagcc caaacttaaa taaaacttat
     2221 acagagttac actttcttac ataacgcctg ctaaattatg agtgttttct aaaccgcccc
     2281 cataatttgc agtcattttg aaaaggaagt cattatgtcg ttctggaaag ttgcagcagc
     2341 gcaatatgag cctcgtaaaa cctcactcac cgaacaggta gctcatcatc tggagtttgt
     2401 gagggccgcc gccaggcaac aatgtcagct gttagttttt ccatcgcttt ctttactggg
     2461 gtgtgattat tcgcgacgtg cccttcctgc cccacccgat ctttcactgt tagacccgct
     2521 ttgttatgcc gcaacgacct agcgaatgac catcattgcc ggccttcccg ttgaatataa
     2581 cgatcgcttt attcgtggaa ttgcggtgtt cgcaccgtgg cgaaaaacgc ctgggaacta
     2641 ccatcagagt catggtgcat gtctgggccg tcgttccaga accatcacgg tggtcgatga
     2701 acaaccgcaa ggtatggata tggacccgac ctgttcactg tttacgacag ggcaatgtct
     2761 gggagaaccc gacctgctgg cgtcggcccg tcgcttacaa tttttttcac atcagtactc
     2821 gattgccgtg ctgatggcaa atgcccgtgg taacagtgca ctatgggatg aatatggtcg
     2881 tctcatcgtt cgcgccgatc gtggttcatt attgttagtc ggtcagcgtt catcacaggg
     2941 ttggcaaggc gatatcattc cattacgcta ggctttttcg gcctggatca tgccatgttg
     3001 cgcattatcg atacagaaac ctgcggtttg cagggaggga tcgttgagat tgcctctgtt
     3061 gatgtcattg acggaaaaat cgtcaacccc atgagcctcc tggtgcgccc cgatcgtcct
     3121 attagtccac aagcgatggc gattcatcgc atcaccgaag ccatggtcgc cgataaaccg
     3181 tggattgaag atgtgatccc acactattac ggtagtgaat ggtatgtcgc gcacaacgcc
     3241 agctttgacc gccgcgtact gcctgagatg cccggtgagt ggatttgcac tatgaaactg
     3301 gcccgtcgtt tgtggccagg gctcaagtac agcaatatgg cgttatataa aacacgcaag
     3361 ctcaatgtac agacaccgct gggcctgcat catcaccgcg cgttgtatga ctgttatatc
     3421 accgccgcgt tgcttatcga tattatgaac acctccggct ggacggcaga acagatggcc
     3481 gatatcaccg gacgtccgtc gttgatgaca accttcacct ttggcaaata ccgtggcaaa
     3541 gcggtttccg acgttgccga acgctatccg ggctatctgc gctggttatt taataacctg
     3601 gacagcatga gcccggagct gcgtttaaca ctgaaacatt atctggaaaa tacttaatcc
     3661 gcaggcgtag cgggtaatgt tccctgcgcc agcgcgacca gaaaagcata ttccatcgct
     3721 acgccttcgt acgatttaaa gcggccagat ttaccgccat ggcctgagtc catgtcggta
     3781 cagagcaata aaagatggtc atcggttttc agctcgcgca atttagcgac ccattttgcc
     3841 ggttcccaat attgcacctg agaatcgtgt aaaccggtcg ttaccagtaa atgcggataa
     3901 gcctgtgcgg tgacgttgtc atacgggctg tagcttttca tgtactcgta atattgcgga
     3961 tcctgcggat ttccccactc ttcaaactca ccagtggtaa gaggaattga ttcatcaagc
     4021 atcgttgtta caacatcaac aaacggtacc tgggcgataa cgccgtggaa taattccggg
     4081 cgttgattaa ttgcaacgcc catcaacatg ccccccgcac ttccgcccat cgcataacag
     4141 agcgaaggag agccatagcc cagttttaac aatgcatcgc aggcatcaag ataatcatta
     4201 aacgtatttt tcttcttcag aaatttaccg tcttcgtacc attgttgccc cagctcacca
     4261 ccgccgcgaa catggacaat ggcgtagaca aagccacgat ctaacaaact caagcggcta
     4321 aaactgaaat cggcatcaat acttgcgccg taagaaccat agccatacac cagcaacggg
     4381 ttgtgtcctt tgcgaaaatg tttgcgatga tagaccagcg aaaccggaac ttcgacgcca
     4441 tcacgggcga ctatccacag gtgttcactg cggtaattcg ccgcatcaaa accaggcact
     4501 tctgtttgtt ttaatacacg acgctcaccg gtatccatat ccagttcaaa caaagtgtct
     4561 ggtgtggtca tggaagaata accataacgc aatcgcgcgg tttcaggttc tggattgtag
     4621 gcaatccagg tcacataggc cggatcatca aaggcaatgc caatgacttc ccgggtcttg
     4681 cggttaattt ggcgcaaact ggttaacccg cgctgacgct cttcaaccac cagccagtcg
     4741 gtaaacagcg taaacccttc cagcatgatg ttttcgcgtg gcggaattaa ctcttcccac
     4801 tgttgctcat cacgcatacg ggtacggtat aagccaaagt ttttgccgtg gcgattggaa
     4861 cgcagataaa aacgatgctg gtagtgatca aggctgtatt cgtgatcttt gcggcgcggc
     4921 agaaaaacaa acggctcggc atcggccatt tccgcgtcca gcaggcgaac ttcactggtg
     4981 gtggcgctgg ccagatgaat gacgacatag tgcttcgacg tcgttttatg caggctgaca
     5041 taataggtat cgtctttttc ttcgtagatc agtgtatctt gcgatgctgg cgtaccgatg
     5101 gcgtgacgcc agacctgata aggcagcagc gtcaccggat gcttgcgaac atagtagaaa
     5161 gtccaggagt catttgccca gacaaagctg ggttcaacgt tatccagcag ttccgggtac
     5221 cagttacctg tttccagatt acgaaaacga atgccgtact ggcgtcggga aagaaaatct
     5281 tctgccagcg ccataatggt gttatcgggc gtaatcgcca ttccgcccat cgaataaaac
     5341 tcactatgag ctgcgcgctt gttggcatcg agcaatgttt cccactcatc ccactcttcg
     5401 ctgaatgccg attgacgctg gtagatagca tattcacagc ctggttcata aatatgccga
     5461 tagcggtagc cattttttat gtagggcgca gaaacttctc tttgcggaat gcggtcgatg
     5521 atttccttta agatgcgatc ctgcaaggct tgttgtgagg ccatcacccg atgaccgtaa
     5581 ctattttctt gttgcaggta gtccaggact tttggctgag aacgcgtatc gtcccgcagc
     5641 cagtagtaat tatcgatgcg cgtatcgcca tgaagcgtca tggcgtgggg aatgcgggcg
     5701 gcttttggta gcatgttatt gttctttctg gttggaaaca tcttataagg gtggcaaaac
     5761 tcaccgggga tgcaagcgaa acagggtcgt cattgcttag atgatgacag gtaatggcgc
     5821 ggatatcgaa tgttatgcag acagagaaaa tcagcctgtt caaatggctg tgcggttctg
     5881 gatagcccga aatagtcaac ttcaggctat ccagagagcg gaattattcc gccaaagtgc
     5941 gtttttgctg ttcgagatcg cgttcaatgc cttcacgaac atcctggggg attttcagcg
     6001 cgtcacccag tgcattcagg taactgcgtt ccataaaatg gtcaatatca atagccgcgc
     6061 agctcaggaa atagatttcc agcgcctctt cttcattgcg gaccccggta gccaggcgtt
     6121 gtggatccag cggttgttcg attgcctgct caatgagtac acgcccctgc tcttccacgc
     6181 cggcttcacg caattgctgg tcgattgccg cacgttcatt ggcatcaata tgaccatcac
     6241 ttttagcggc aaagaccagc gcaaggatca aacgttccgt acgctcatcc agcggcgtac
     6301 tttgcgcgcc aaactgcggt tcgtcctgat gcgccgcgcg aattttatct ttgtatttat
     6361 tccacagcac cgtacccgct accgctccgc cgccaaccag taacgcgttg gtgccatatt
     6421 tcgtaagaag tttacgtgct gatttatttg cgaccagcag cccagccagt ccgcctaatg
     6481 cgcctgacac taacagtttg cccaatccct gatccgcaga cgaggaggta gaagaactgc
     6541 tttgcccaag cagggattgc agttgattta accagttagc catatttgct cctcaataac
     6601 catttttagt gcctgtagca tagcggatga ggatgtcaga aaatgtatat cgagacaaaa
     6661 gatgcgcaaa taagcggggc ggtaggccgt tattcaaaga aattcgcgcc aggtaaggta
     6721 tttactgtat atcaggtgat aagggtattt taggtgaaag tagtttgtcg ttttgagtgg
     6781 cattgtttga tgctgattgc ccgatgcgac gctgttgcgt cttctcaggc ctacgattgt
     6841 ggtgcgttct gggacggaca aggcactcgc gccgcatccg ccaacggtca atcgaattat
     6901 cagaagccat taacgccgct gatattccgc cattcccgct ttgcagtcca cgcagacctg
     6961 gtaatgaata tcggcacttt taccacgcac ggttagcggt actgaccatt tatcatcttt
     7021 accctgaatg tcctgcaaac tgacccaggc cacgggatcg gcctgaccga caattttttg
     7081 atcatctgcc cagcgcgcca cgcgattttg ttgataatca cgttttacgc tcgcggcaat
     7141 tccggcagca tccagacctt cacactttgg gaaagtgacc gacttgctgg tttcattatt
     7201 ggcagcgaaa actgatgcgc aggcagaaac caacaaccgc cctaaaaacg cccctctttt
     7261 tttcatgttt ttctccatag cacaatgatt caggagaaag catggtacaa attgtcagga
     7321 gcgcaagttg cttcaggccg cgtgtgaggc atcttcctct tcggattcag caccgacgac
     7381 ctgtggggac ggtaatttgc cggttttcag aatggtgctc agcacctctt tttgttctgc
     7441 cagccatagc gaaagggctt cacgttgctc gtcttccatt tgcactggag aatttgtcag
     7501 ctaggtgtca agcagatccg ccgtatcaag cattttgtca taagcatcgg cttccttttt
     7561 gctggtaaac gacattttct cttcgccctc acgaatgact acgtatttaa cttcaaccgc
     7621 catttgcagc ctctcataat aactgaaatt ttatacagta tatttctttt cggttgagaa
     7681 atcaacatca gcaataaaga cacacgcaaa cgttttcgtt tatactgcgc gcggaattaa
     7741 tcaggggata ttcgttatga cgttattagg cactgcgctg cgtccggcag caactcgcgt
     7801 gatgttatta ggctccggtg aactgggtaa agaagtggca atcgagtgtc agcgtctcgg
     7861 cgtagaggtg attgccgtcg atcgctatgc cgacgcacca gccatgcatg tcgcgcatcg
     7921 ctcccatgtc attaatatgc ttgatggtga tgcattacgc cgtgtggttg aactggaaaa
     7981 accacattat atcgtgccgg agatcgaagc tattgccacc gatatgctga tccaacttga
     8041 agaggaagga ctgaatgttg tcccctgcgc tcgcgcaacg aaattaacga tgaatcgcga
     8101 gggtatccgt cgcctggcgg cagaagagct gcagctgccc acttccactt atcgttttgc
     8161 cgatagcaaa agccttttcc gcgaggcggt tgccgccatt ggctatccct gcattgtaaa
     8221 accggtgatg agctcttccg gcaaggggca gacgtttatt cgctctgcag agcaacttgc
     8281 tcaggcatgg gaatacgctc agcaaggcgg tcgcgccgga gcgggccgcg taattgttga
     8341 aggcgtcgtt aagtctgact tcgaaattac cctgctaacc gtcagcgcgg tggatggcgt
     8401 ccatttctgt gcaccagtag gtcatcgcca ggaagatggc gactaccgtg aatcctggca
     8461 accacagcaa atgagcccgc ttgcccttga acgtgcgcag gagattgccc gtaaagtggt
     8521 gctggcactg ggcggttatg gtctgtttgg tgtcgagctg tttgtctgtg gtgatgaggt
     8581 gattttcagt gaggtctccc ctcgtccaca tgataccggg atggtgacgt taatttctca
     8641 agatctctca gagtttgccc tgcatgtacg tgccttcctc ggacttcctg ttggcgggat
     8701 ccgtcagtat ggtcctgcag cttctgccgt tattctgcca caactgacca gtcagaatgt
     8761 cacgtttgat aatgtgcaga atgccgtagg cgcagatttg cagattcgtt tatttggtaa
     8821 gccggaaatt gatggcagcc gtcatctggg ggtggtactg gctacttcag agagtgttgt
     8881 tgacgccatt gaacgcgcga agcacgccgc cggacaggta aaagtacagg gttaaacccg
     8941 gcaaaaaaac gctacaaaaa tgcccgatcc tagatcgggc attttgactt ttacagctta
     9001 gcgccttcta cagcttcacg cgccagctta gtaatgcggt cgtaatcgcc cgcttccagc
     9061 gcatctgccg gaaccagcca ggaaccaccg atgcacagca cgcttttcag tgccaggtag
     9121 tcacggtagt tagccggaga aataccaccc gtcgggcaga aacggacctg ggagaacgga
     9181 cccgcgatcg cctgcagggc tttcacgccg ccgttagctt cagccgggaa gaatttgaac
     9241 tctttcaaac cgtagtccat acccagcatc agttcggaaa cagtgctgat ccccggaatc
     9301 agaggaatag tcccttcggt agcagctttc agcagcggct cggtcagacc cgggctaatt
     9361 gcgaactgtg cacccgcttc agtgacttct gccagctgct gtggattcag caccgtaccg
     9421 gcacccacaa tcgcttcagg cacttctttg gcgatagcac ggatagcgtc aactgcacac
     9481 tcggtacgca gagtcacttc cagaacgcgc accccaccag caaccaacgc ttttgccatc
     9541 ggcaccgcgt gttccagttt ttttaccacg ataaccggta caaccgggcc ggtggtcagg
     9601 attgattctg cacttgtttt ccagtttttc atcagagttt tctctcgcct gattacaaat
     9661 ttgtcgtctt aaaaagtgat acaggttgcg ccctgttcgg caccggacag tttttcacgc
     9721 aaggcgctga ataattcacg tcctgttccc acgcgtgacg cgctcaggtc aggaatgtgc
     9781 ggttcgcgag cagccagttc cgcttcgtct accagcagtg tcagttcgcc tgtctgtcca
     9841 ttcacacgaa tgatgtcccc gtcgcgcact tttgccagca gcccgccatc gtaggcttct
     9901 ggtgttacgt ggatagctga cggcacttta cctgaagcgc cggagagtcg tccatcggta
     9961 actaacgcaa ttttgaaaca ccggtccaat aatacaccaa gtggcggcat gagtttatgt
    10021 aattctggca ttccgttcgc ttttggcccc tgatgacgga caacaacgac acaatcgcgg
    10081 tccagcaaac ccgcttcaaa ggccggcata acgtcatgct ggctttcaaa aacaaccgct
    10141 ggcgcttcaa tcacctggtt ctcaaccggc acggcagagg ttttcataac cgcacggccc
    10201 aggttaccgc ttaacacttt tgtcccacca tgatgagaga aaggttgttc gaaggaagcg
    10261 atcacattgc tgtcgagtga tttttccgcc ccttcccgcc agtccagttc accattattc
    10321 agccatggtt caagggtata acgagacaga ccaaagcctg ccaccgtatt gacatcttca
    10381 tgcagcaggc ctgctttgag cagttcacgc accagaaccg gtacgccacc tgccgcctgg
    10441 aagtggttaa tatcggccgg accgttcggg tagagacgtg ccatcagcgg tacaacatca
    10501 gaaaggtcag agaagtcatc ccagttaatc tgaataccgg ccgcgcgcgc catcgccacc
    10561 aggtgcatgg tgtggttagt ggaaccaccg gtcgccagca gtgcaacgat accgttcacc
    10621 accactttct catcgatcat cttaccgatc ggcatccatt cattaccatt accggtcatg
    10681 cgtgtaacct gacgcgcagc ggcagcggtc aaagcatcgc gcagcggaga atccggatga
    10741 acaaaagaag agcctggcaa ctgcatcccc ataaattcca ccaccatctg gttggtgttg
    10801 gcagtaccgt agaaagtaca tgttcccggc gcatggtaag acgcggcttc tgactccagt
    10861 aaggccatgc ggtccacttt accttcggca taaagctggc gaatacgcac tttttcttta
    10921 tttggcaaac cgcttgccat cggtccagac ggcacaaaca ccgcaggcaa atgaccaaac
    10981 gacagggctg ccatcgtcag acccgggaca atcttgtcgc acacaccgag gaacagagca
    11041 ccatcaaaca tgttatggga cagccccacc gccgcagaca tcgctatcac ttcgcggctt
    11101 agcagcgaca attccattcc atcctgcccc tgggtgacac catcacacat cgccggaaca
    11161 ccgcccgcaa cctgaccaac cgcattcgct tcatgcaggg ctttacgaat gatttctgga
    11221 tagtgttcat aaggctggtg cgcggagagc atgtcgttat aggaggtgat gatggcgata
    11281 ttgttacgca acatgctttt caaagaggct ttgttttctg gctggcaggc agcgaaaccg
    11341 tgtgccaggt taccgcatgc caactgcgaa cgatgaacgg tcgacgtttt cgcttgttct
    11401 atccgggcga gataagcaga gcgagtctcg cgcgaacgtt caatgattcg atttgttacg
    11461 cgtaacaatt gtggattcat aaaggctcct gaaattgagt tgtcagagca ggatgattca
    11521 caacgcgttt cattcagagg atttatgagt gaaacgcctg taaccggagc tcatagggca
    11581 aaaacgtttc agtcagtgta ataaaaaaag cctcgtgggt gaatccgcac gaggcctgaa
    11641 agtgtaaaaa ttgttctaca atctgtgcaa gatcatgtta ccggtaaaat aaccataaag
    11701 gataagcgct gatattactc aaactcattc caggaacgac catcacgggt aatcatcgcc
    11761 accgaggcaa cgggtcccca ggttccggcc tgatacggtt tcggcgcatc attgtccatc
    11821 gcccacgcct cagtaatgga gtctacccat ttccaggctt cttccacttc gtcgcgacgt
    11881 acaaacagtg cctgaatacc acgcatggtt tccagcagca aacgttcata ggcatccgcc
    11941 agatgcgtct gattaaaggt ttctgaatag ctcagatcca gcttggtgat ttgcaggtta
    12001 tgtttgtggt caaggccagg aactttattc agtacctgga tatccacgcc ttcatcaggt
    12061 tgcagacgga tagtcagttt attctgcggc agatcctgcc acgactcttt aaacagattc
    12121 agttcaggtg ttttgaaata gaccacgact tcagaacatt tggtcggcag acgtttacca
    12181 gtacgcaggt agaatggcac accggcccag cgccagttat caatgtcgac gcggatcgcc
    12241 acgaaggttt ctgtattgct gcttttgttc gcgccctctt cttccagata tcccggcact
    12301 tttttgccct gggcgaagcc cgcagtatat tgcccgcgta cggttttttc gcgtacgttg
    12361 gagcggtcga tgcggcgcag ggacttcagt actttcactt tttcatcgcg gatgctgtct
    12421 gcgctcaggt cagatggcgg agacatcgca atcatgcaga gaatttgtag caggtggttc
    12481 tgaatcatgt cacgcatctg accggcttta tcaaaatagc cccagcgccc ttcgatcccc
    12541 acttcttctg ccacggtaat ctcaacatga tcaatggtgc gattgtccca gttattcaca
    12601 aacagggagt tagcaaaacg cagcgccaac aggttcagca ccgtttcttt accaagatag
    12661 tggtcgatac ggtaaacctg gcactcctcg aagtattcgc caacctgatc attgatttcc
    12721 tgcgaggtcg ccagcgacgt ccccagcggt ttctccatga ctacgcgtgc cggtttagca
    12781 ttcagttttg cctcgccaag ccctttgcaa attgcgccaa aagtgctggg cggcatggca
    12841 aagtagttaa tagtgatacg atttttttga tccagcatcg cgccgagacg gttgaatgca
    12901 gcagtgtcat tgacatcgag attacaaaaa tccagacgtg cactcagggt gtcccataaa
    12961 ccttcatcaa tggtttcttt catgaaagtt tcgagcgcct cgcggacaac tttggtatat
    13021 gccgctttat cccagtcagc acgccctacg ccgataatcc gggtgtccgg gttgagctga
    13081 ccgacttttt ccagttgata cagggaaggc agcaatttac gacgcgcaag gtcgcctttc
    13141 gcgccgaaaa tgaccaggtc acaggcctgg gctgtttgcg ttaccgccat gtcattctcc
    13201 ttaagttaac taacccggta cttaagccag ggtatacttg taattttctt acggtgcact
    13261 gtactgcttt tacgagcttg cgaaaactgt aaacgcttat ccacccgtgc gattacggga
    13321 aaagcgcgca aagcgcggca aaactgataa aaaaatcatc gttttccatt cggtaaaaat
    13381 ctgacactga tcatgttatg aaaaaaaata acaacttttt tatctgcttt tgtcattaac
    13441 acggcacaca ggcgtaatat ctgacaaaac tgcatttcga tttctttcag tgcggaaatc
    13501 gtcattaccc gtgagtctct ttacatcatg aatatgctgg aaaaaatcca gtctcagctg
    13561 gaacatttga gcaaatcaga gcgcaaagtt gccgaggtca ttctggcttc gcccgataac
    13621 gcgatccatt cgagtattgc tgctctggcg ctggaagcca atgttagcga accgacggtg
    13681 aatcgtttct gtcgcagcat ggacacgcgc ggttttcctg attttaaact tcatctggca
    13741 caaagtctgg cgaatggcac tccctatgtt aatcgcaacg tcaatgaaga tgacagcgtt
    13801 gaatcataca cggggaaaat atttgagtcc gcaatggcaa cgctcgatca tgtccgtcat
    13861 tcactggata aatctgccat caaccgcgcc gtcgacttgc tcactcaggc aaaaaaaatc
    13921 gcctttttcg gattaggctc ttctgccgcc gttgcccacg atgcgatgaa taagttcttt
    13981 cgttttaatg ttccggtggt gtactccgat gatatcgtgc tgcaacgcat gagttgtatg
    14041 aattgtagcg acggagacgt ggtggtgctg atttctcaca caggaagaac aaaaaatctg
    14101 gtcgagctgg cgcagctggc acgcgaaaac gacgccatgg tgattgccct cacctctgcg
    14161 ggtaccccgc tcgcccggga agcaacgctg gcaattaccc tcgacgtacc ggaagatact
    14221 gacatttata tgcccatggt ttctcgactt gcacagctga ccgtgataga tgtgctggcg
    14281 acaggattta ctttgcgacg cggtgcaaaa ttcagagata acttgaagcg ggtcaaggaa
    14341 gcgctgaagg aatcgcgttt tgataagcag ttacttaatt taagtgacga tcgctaaaag
    14401 cgactgtcac tgtcctaatc ttatacgaca tccgaatgag attaatttat cgccatcgcg
    14461 gcgttatttc attcggattt catgttcaag caacacctgg ttgtttcagt caacggagta
    14521 ttacatgtcc aaaaggcttc gcagaacaaa aatcgttacc acgttaggcc cagcaacaga
    14581 tcgcgataat aatcttgaaa aagttatcgc ggcgggtgcc aacgttgtac gtatgaactt
    14641 ttctcacggc tcgcctgaag atcacaaaat gcgcgcggat aaagttcgtg agattgccgc
    14701 aaaactgggg cgtcatgtgg ctattctggg tgacctccag gggcccaaaa tccgtgtatc
    14761 cacctttaaa gaaggcaaag ttttcctcaa tattggggat aaattcctgc tcgacgccaa
    14821 cctgggtaaa ggtgaaggcg acaaagaaaa agtcggtatc gactacaaag gcctgcctac
    14881 tgacgtcgtg cctggtgaca tcctgctgct ggacgatggt cgcgtccagt taaaagtact
    14941 ggaagttcag ggcatgaaag tgttcaccga agtcaccgtc ggtggtcccc tctccaacaa
    15001 taaaggtatc aacaaacttg gcggcggttt gtcggctgaa gcgctgaccg aaaaagacaa
    15061 agcagacatt aagactgcgg cgttgattgg cgtagattac ctggctgtct ccttcccacg
    15121 ctgtggcgaa gatctgaact atgcccgtcg cctggcacgc gatgcaggat gtgatgcgaa
    15181 aattgttgcc aaggttgaac gtgcggaagc cgtttgcagc caggatgcaa tggatgacat
    15241 catcctcgcc tctgacgtgg taatggttgc acgtggcgac ctcggtgtgg aaattggcga
    15301 cccggaactg gtcggcattc agaaagcgtt gatccgtcgt gcgcgtcagc taaaccgagc
    15361 ggtaatcacg gcgacccaga tgatggagtc aatgattact aacccgatgc cgacgcgtgc
    15421 agaagtcatg gacgtagcaa acgccgttct ggatggtact gacgctgtga tgctgtctgc
    15481 agaaactgcc gctgggcagt atccgtcaga aactgttgca gccatggcgc gcgtttgcct
    15541 gggtgcggaa aaaatcccga gcatcaacgt ttctaaacac cgtctggacg ttcagttcga
    15601 caatgtggaa gaagctattg ccatgtcggc aatgtacgca gctaaccacc tgaagggcgt
    15661 tacggcgatc attaccatga ccgaatcggg tcgtaccgcg ctgatgacct cccgtatcag
    15721 ctctggtcta ccaattttcg ccatgtcgcg ccatgaacgt acgctgaacc tgactgctct
    15781 ctatcgtggc gttacgccgg tgccctttga tagcgctaat gacggcgtag cagctgccag
    15841 cgaagcggtt aatctgctgc gcgataaagg ttacttgatg tctggtgacc tggtgattgt
    15901 cacccaaggc gacgtgatga gtaccgtggg ttctactaat accacgcgta ttttaacggt
    15961 agagtaagta cgttgccgga tgcggcgaaa acgccacatc cggcctatag ttcaatgata
    16021 gttcaacaga tttcgaatat tctgaagcaa acttgaactt atcatcaggc gaaggcctct
    16081 cctcgcgaga ggctttttta tttgatggga taaagatctt tgcgcttata cggctggatt
    16141 tcgcccggtt tgcgagtttt cagcaatttt agtatccagg tgtattgttc tggtcgcgga
    16201 ccaacaaaaa tctcgacttc ttcattcatc cgccgctcaa tcgtatgatc atccgcctct
    16261 aacagatcat ccatcggtgg gcgcacctga atcgtcagac gatgcgtctt gccatcataa
    16321 atcggaaaca gcggtacaac gcgcgcacgg catactttca tcaaacgacc aatcgcgggc
    16381 aacgtcgctt tataggtggc aaagaaatcc acaaattcgc tgtgctctgg gccatgatcc
    16441 tgatcgggta aataatatcc ccagtacccc tgacgtaccg actggatgaa tggtttaata
    16501 ccgtcatttc tcgcatgcag acgaccgcca aagcgacgac gcaccgtgtt ccagacataa
    16561 tcaaaaaccg ggttgccctg attatggaac atcgctgcca ttttctgccc ttgcgaggcc
    16621 atcagcatgg caggaatatc gacggcccaa ccgtgcggca ccagaaagat aactttctcg
    16681 ttattacgcc gcatctcttc gatgatctcc agcccttgcc agccaacgcg cggctgaatt
    16741 ttctccggcc cgcgtattgc caactcagcc atcattgcca tcgcttgcgg cgcggtggca
    16801 aacatctcat caacaatcgc tt
//
LOCUS       DAZXUK010000067        16698 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZXUK010000067 DAZXUK010000000
VERSION     DAZXUK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16698
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.067"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(136..1626)
                     /gene="glpK"
                     /locus_tag="ACH3L8_001756"
     CDS             complement(136..1626)
                     /gene="glpK"
                     /locus_tag="ACH3L8_001756"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFT4084904.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            complement(1666..2511)
                     /gene="glpF"
                     /locus_tag="ACH3L8_001757"
                     /pseudo
     CDS             complement(1666..2511)
                     /gene="glpF"
                     /locus_tag="ACH3L8_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            2937..3182
                     /gene="zapB"
                     /locus_tag="ACH3L8_001758"
     CDS             2937..3182
                     /gene="zapB"
                     /locus_tag="ACH3L8_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFT4084905.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            complement(3267..3752)
                     /gene="rraA"
                     /locus_tag="ACH3L8_001759"
     CDS             complement(3267..3752)
                     /gene="rraA"
                     /locus_tag="ACH3L8_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFT4084906.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(3845..4771)
                     /gene="menA"
                     /locus_tag="ACH3L8_001760"
     CDS             complement(3845..4771)
                     /gene="menA"
                     /locus_tag="ACH3L8_001760"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFT4084907.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            complement(4838..6169)
                     /gene="hslU"
                     /locus_tag="ACH3L8_001761"
     CDS             complement(4838..6169)
                     /gene="hslU"
                     /locus_tag="ACH3L8_001761"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFT4084908.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            complement(6179..6709)
                     /gene="hslV"
                     /locus_tag="ACH3L8_001762"
     CDS             complement(6179..6709)
                     /gene="hslV"
                     /locus_tag="ACH3L8_001762"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFT4084909.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            complement(6802..7761)
                     /gene="ftsN"
                     /locus_tag="ACH3L8_001763"
     CDS             complement(6802..7761)
                     /gene="ftsN"
                     /locus_tag="ACH3L8_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFT4084910.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            complement(7853..8878)
                     /gene="cytR"
                     /locus_tag="ACH3L8_001764"
     CDS             complement(7853..8878)
                     /gene="cytR"
                     /locus_tag="ACH3L8_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFT4084911.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            complement(9034..11232)
                     /gene="priA"
                     /locus_tag="ACH3L8_001765"
     CDS             complement(9034..11232)
                     /gene="priA"
                     /locus_tag="ACH3L8_001765"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFT4084912.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            11435..11647
                     /gene="rpmE"
                     /locus_tag="ACH3L8_001766"
     CDS             11435..11647
                     /gene="rpmE"
                     /locus_tag="ACH3L8_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFT4084913.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            complement(11708..12316)
                     /locus_tag="ACH3L8_001767"
     CDS             complement(11708..12316)
                     /locus_tag="ACH3L8_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFT4084914.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(12500..12817)
                     /gene="metJ"
                     /locus_tag="ACH3L8_001768"
     CDS             complement(12500..12817)
                     /gene="metJ"
                     /locus_tag="ACH3L8_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFT4084915.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            13094..14254
                     /gene="metB"
                     /locus_tag="ACH3L8_001769"
     CDS             13094..14254
                     /gene="metB"
                     /locus_tag="ACH3L8_001769"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFT4084916.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            14257..16689
                     /gene="metL"
                     /locus_tag="ACH3L8_001770"
     CDS             14257..16689
                     /gene="metL"
                     /locus_tag="ACH3L8_001770"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFT4084917.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
BASE COUNT         3794 a         4475 c         4461 g         3968 t
ORIGIN      
        1 agtctgcgtg atgccggatg cggcgtgaac gccttatccc gcctacacaa aatggcattg
       61 gctcgtaggc ctgataagac gcgtaagcgt cgcatcaggc gatcagcatc aggcaatcga
      121 ttcaggtgcc tgacattact cgtcgtgttc ttcccacgcc atcgcgcgtt tgaccgcttt
      181 tttccagcct gcgtaacggt aattacgctc agtggtttcg atgcctggac ggaactcgcg
      241 ctcaatcacc gctttctctt gcagctcgtc gaggttctgc cagaagccaa ccgccaggcc
      301 tgcgagatag gccgcaccca atgcggtgac ttcgcgcact tccgggcgct caacgcgggt
      361 gccgagaata tcggactgga actgcatcag gaaattgttt gctactgcgc caccatccac
      421 gcgcagggcg tgcagacgga taccagagtc ggcctgcatc gcttccagca cgtcacgcgt
      481 ctgataagca atagactcca gcgtcgcgcg tataatgtgg ttagcgttca ccccacgagt
      541 cagaccgaaa atcgccccgc gcgcatacgg gtcccagtac ggcgcaccca gcccggtaaa
      601 tgccggaacc acatacacac cattggtgtt ttgcactttg gtggcgaaat attcggaatc
      661 gtaggcgtcg ttaatcaact tcatctcatc gcgcagccac tgaatggatg cgcctgccat
      721 aaacaccgca ccttccaacg catagttcac ttcgccagtc gggccgcagg cgatggtggt
      781 cagcaggccg ttttctgatt tcaccgcttt ctcgccagtg ttcatcagca taaagcagcc
      841 agtgccatag gtgttcttcg ccatcccttc tttcacgcac aactgaccaa acagcgcggc
      901 ctgctggtca ccggcgatcc cggagattgg aatacgcgtg ccgcctttgc cgccaatgtt
      961 agtctgaccg tatacttcgg aagaacgacg cacttctggc agcatctcgc gcggaatatc
     1021 cagcacttcc agcattttgt cgtcccagtc cagggtatgg atgttgaaca acatggtacg
     1081 agaggcgttg gtgtaatcgg tcacatggac acggccctga gtcattttcc agataagcca
     1141 cgtatcaacc gtaccaaaca gcaattcacc acgacgtgca cgctcgtgag agccttccac
     1201 atggtcgagg atccacttca ctttggtgcc agaaaagtac gggtcaatca ccagaccggt
     1261 attgctgcgg atataatctt ctaaaccgtc acgttttaaa tgctcgcaga tttctgcggt
     1321 acgacggcac tgccagacaa tggcgttata gataggcttg ccggtttctt tttcccagac
     1381 aatagtggtt tcacgctggt tcgtaatacc gatagctgca atttgatcgg aactgatatc
     1441 ggctttcgcc agcacttcta ccagcgtgga gctttgggtg gcccagattt ccattgggtc
     1501 gtgttctacc caacctggtt ttgggtagat ttgctcaaat tcgcgctgcg acacgctaat
     1561 gatattggca tcgtgatcca ttacgaccgc gcgggagctg gtggtgccct ggtcgagcgc
     1621 aacgatatat ttttttcagt catgtttaat tgtcccgtag tcatattaca gcgaagcttt
     1681 ttgttctgaa ggagttgtgg tttccttttc ttccacaaca cagatatcgc aaggcaaatg
     1741 gcgaccaatc agtttgcggt aggcaaatgc acctacaatc gcgccaacga tagggctgaa
     1801 aagcggcacc aggaagtaag gaatgtctct gccgccggta aaggcgacat tgccccagcc
     1861 cgccagctag gcaaagactt tcggaccgaa gtcacgcgct gggttcatgg caaaacctgt
     1921 caatgggccc atagatgcgc caatgaccgc aatcagtaga ccaatcagca agggagccaa
     1981 agggccgcgt ggtacaccgt tgccatcgtc cgttaacgcc aggatcagcc ccatcagaat
     2041 agcggtaatc accatctcaa ctgcgaaagc ctgcacaaaa ttgatatgag gattagggta
     2101 agtagagaaa gtgccagcca gatcaacact ttcaacgctg ccgcgaacaa tgtgatgagt
     2161 ctgctcgaag tcgaaaaata aattgtagta aagcccgtaa actaaagccg cagcgcagaa
     2221 agcgccggca acttgtgaaa cgataaaagg aataactttg cgcttgtcga aacaggcaaa
     2281 cagccacaat gcaatggtaa cagcgggatt aagatgcgcg ccggaaaccc ctgcggtcag
     2341 gtagatggcc attgccaccc ccagtcccca aatgacactg atttcccact gaccaaaaga
     2401 cgcaccagcg acttttagtg ctgcaacgca acccacaccg aaaaaaatca acaacccggt
     2461 accgaggaat tcagcaatgc actggccttt caaggttgat gtttgactca taatcggatc
     2521 ctgaagagtt aatgtttgtt gtatgcgtga aagtcacgga cctccacgat gcttgtaggc
     2581 atgctgttaa cttatcgtta acgagcaaaa acgagaaata tcgaacttaa aatgtgtgtg
     2641 cttcgtcata aaatgagcgt tatcgcgcca tttattccat ctgattgtac atgatgcatg
     2701 tttgttagat cattcgcgca acaaatttat taacattttc gattgatgtg gattatgtca
     2761 ctaggagaag gatgaaaagt gtggcaaacc gtaatctgca aggcgtgccg ctggacagcg
     2821 atggcgcggc ttcatacaat cggagctaac taaagtgcgc tcgtatttat taaggcgtca
     2881 ccggtaatcg ggacgaggat tttttatcca tcaacgcctt gcaattcagg agaggtatga
     2941 caatgtcatt agaagtgttt gagaaactgg aagcaaaagt acagcaggcg attgatacca
     3001 tcactctgtt gcagatggaa atcgaagagc tgaaagaaaa aaacaactca ctgtcgcagg
     3061 aagttcaaaa tgcccagcat cagcgcgaag agctggagcg tgagaacaac catctgaaag
     3121 aacagcagaa cggctggcag gaacgtctgc aggccctgct gggtcgcatg gaagaggtct
     3181 gattacgctt catctttcgc gtaatttctt tactgtgtag gccaacaggt aacgcagaaa
     3241 aaaggcacct tgcggtgcct ttcttatcat tcaatatcca gcggatcttc tgaaagaata
     3301 atcccggtat tgtcggcata aagatggtcg ccggagaaga aggtgacacc gccaaaattg
     3361 acgcggacat cgctttcgcc aatgccttcg ccagcggcac caaccggaat tgccgccatc
     3421 gcctggatgc cgatatccaa ctcttccagg tcatctacct gacgcaccgc gccgtaaatg
     3481 accagacctt cccattcatt ttgtactgcc agacgcgcca gttcagcatc gaccagtgcg
     3541 cgacgaacag aaccaccgcc atcgacgaca agaacacgac cacggccatt ctgttcgagc
     3601 agatcgtaca gcaacccgtt gtcctcgaaa cattttaccg tgattatttg tccgccaaac
     3661 gacgcccgtc cgccaaagtt ggagaacagc ggttccacga cgttaacatc ttcttgatag
     3721 atgtcacaaa gctcggaagt atcgtatttc ataggcttaa cattcagttg ctgcgagaat
     3781 tttcagtata tcgcgctatg tgggctgttg gcaaaatcat caattgttaa ttgatatttg
     3841 tcagttatgc tgcccactgg cttaggaata tccctaaaac aaacagcagg ttagtcagta
     3901 acgctccctt gacagtacgt tccagcattg gtcgcatcgc caccgggtcc atttcccgca
     3961 tcacataacg ggcttgcttc accagtaatg gtgccgccag caggaacagc cagccccaca
     4021 ggctatgcag cgaaaagaga ttaaacagcg ccagacacac cagcgagccc atcagcaggc
     4081 aggcatgata acgacgcgcg ttcacttcac ctaagcgcac caccagcgtg tttttgccat
     4141 tttcgcggtc gctattgata tcacgcaggt tattaatatt cagtactgcc gttgccagca
     4201 ggccgcatgc ggtcgccgga aggatcagtg ccggaatcaa tgtatgagcc tgtaaatacc
     4261 agctccccat gacactcaac cagccaaaga aaaccagtac ggaaatatca cccagaccga
     4321 tataaccata aggacgattg cccacggtgt aggtgatagc ggcaatgatc gacaacccgc
     4381 caagaatcag gaaaccgaca aaatcggcca gcgtatggca tgccactgca accagtgcca
     4441 gcccggagag acagatgagc acgacggtaa taatgagcgc ccgtttcatc ttttgctggg
     4501 taatgacccc tttttgcatg ccgcgtagcg gcccaatgcg gtcaggttta tcgctgcctt
     4561 ttaccgcatc gccgtaatca ttggcgaggt tagaaaggat ctgtaatagc ccggcggtaa
     4621 ttagtgccag cagggcgacc agcggatcga agtgaccttg ccaccatgcc agcgccgtcc
     4681 cgacgataat tgcagcaaag gcgaggggga gggttttagg tcgtaaactt tccagccacg
     4741 cctgagttcg gctaatttgt tgttcagtca taatacgcgc caataaaaat ggggcctttc
     4801 agccccatca aacaatgatg aaaatgattg aacgcgatta taggataaaa cggctcagat
     4861 cttcatctgc caccaacgca tccagatgtt tgctcacata atctgcgtca atagtgatat
     4921 tttgaccgct taaatcgctg gcgtcgtagg aaatctcttc cattaaacgc tccagaacag
     4981 tgtgtaaacg acgagcaccg atgttttcgg tagattcgtt cacctgccat gccgcttccg
     5041 cgatgcgttt aataccggag tcggtaaact cgatatttac gccttcagtc gccatcagtg
     5101 ctttgtactg cacggtgata gaggcattcg gctcggtcag aatacgctcg aagtcgctgg
     5161 tggtcagtgc ctgcagttca acgcggattg gcagccgacc ttgcagttcc gggatcaggt
     5221 cagacggttt cgcaatctgg aacgcgccag aagcgataaa cagaatgtgg tcagttttga
     5281 ccatcccgtg tttggtggaa acggtgcaac cttctaccag cggcagcagg tcacgctgaa
     5341 cgccttcacg agaaacatcc ggaccggaag actcgccgcg cttacagatt ttgtcgattt
     5401 cgtcgataaa cacgatcccg tgctgctcaa cagcgtcgat agcgtcttgc ttcagctctt
     5461 ccgggttcac cagtttcgcc gcttcttctt caatcagcag cttcatggcg tctttgattt
     5521 tcagcttacg cgctttttgc ttctggccgc ccaggttctg gaacatggac tgcagctggc
     5581 tggtcatctc ttccatgccc ggaggagcca taatttcaac gcccatcggt gctgcggcaa
     5641 gatcgatctc gatttctttg tcatcaagct ggccttcacg cagttttttg cggaatgcct
     5701 gacgagcagc ggaaggttcc tgctgctgtt cagtctgtcc ccagttgttt ttagcaggtg
     5761 ggatcagcac gtcgagaata cgttcttctg ccagttcttc agcgcgataa cggtttttct
     5821 cgatagcctg gacgcgtacc attttcacgg cggcatcggt cagatcgcga ataatagaat
     5881 caacttcctt accgacgtag cccacttcgg taaatttggt cgcttcaact ttgatgaacg
     5941 gcgcattcgc cagcttagcc agacgacggg cgatttcagt tttaccgaca ccggtcgggc
     6001 cgatcatcag gatatttttc ggggtcactt catggcgcag ctcttcgttg agctgcatgc
     6061 gacgccagcg gttacgcaga gcaatcgcca cagaacgctt ggcgttgtcc tggccgatga
     6121 tgtgcttatc cagttcgctg acgatttcgc gtggggtcat ttcagacatg ggagatcctt
     6181 acgctttgta gcttaattct tcgatggtgt ggaaatggtt ggtatagatg caaatgtcgc
     6241 ctgcaatatc caacgccttt tcagcaattt cacgggcgct aagttcagtg ttttctaaca
     6301 gcgcgcgcgc cgcagcctgg gcgtaagggc cgccggagcc gatagcaata agatcgtttt
     6361 ctggctgcac cacgtcaccg ttaccggtga tgataagcga tgcagtttca tccgcgactg
     6421 ccagcagtgc ttcaagtttg cgcagcatgc gatcggtacg ccagtctttt gccagctcaa
     6481 cggcggcttt aaccagatgg ccctgatgca tttccagttt acgttcaaac agttcgaaca
     6541 gcgtaaaagc atccgcagta ccgcccgcaa agcccgcgat gactttgtcg ttgtacagac
     6601 ggcggacctt tttcacgttg cctttcatta cggtattgcc caacgtggcc tgaccatcac
     6661 cagcgatgac cacatggccg ttacggcgta cgcttactat agttgtcacg agctgacccc
     6721 ttggttacga atacagagta caaaccccgt acaaaagtac ggggcataat gcaattatag
     6781 atggggggga ttttgagggt ttcaaccccc ggcggcgagc cgaatgcagt ttgtatgacc
     6841 cgccatcttc aaccgattga gggtgctgtc tgcgttctct ttgcctttca ccgggccaat
     6901 gaccacacga ttccagccat tgttggtggt gattttcgag tcaaatcctt cgaacgccag
     6961 ctgagcacgt accgtctctg cctgttccgc gcctctgaac gaaccgcact gcaccatcca
     7021 gcggcgttcg tcttttttct ccgccgtcgg ttttggcgcg tcagcggcac gagcaaccgg
     7081 cgcagcttgc tgcggcttcg actgcgcggt cgtgtgcgca ggggtttgca gcagatcctg
     7141 gtacggctgc tgagtagaag ctggtttaga ctggcgcggt tgcgcctgta ccggcgcggc
     7201 ttgcgacgta cgcgtctgct gctgccagct ttgttcagtc gtacgcgact gctgagccag
     7261 gcgttgctgc tccgctaatt gctgcgcctg acgctggcgt tgtagcgttt gctgacgctg
     7321 ttccggcgtc tgttcattcc acggaacctc aaccagctgc gttggctgct ggcgcatatc
     7381 agcctgcatt tgttcaagaa gctgacgttg ttctggtgtc agttgctccg gcgttttcac
     7441 ttcaccaccg gcagaaggtt ctgtgggcgc acgcacaccc ggctggcgac tttccagctc
     7501 tttaatgtag cgccagcgtt cttctggttt tggtggtagt ccgtttccgg tcactttctg
     7561 gctttgcagc gtctcggact cttctttctt gtgatgcgta atgaagtaca gaccaccgat
     7621 aaaggtcaca agaacggcgg cagcaatagc gaccatagcg ggagaaaccg caggcagatt
     7681 tcgttgcttt ttccgtgagg tgctcttttt tcgccgcgaa ggtgccggtt ggctgcggcg
     7741 tacataatct cgttgtgcca ctatcgtttc gctgtattta ttcgttcgtc agcccgccat
     7801 gttacttaag cggcgggcct ttgaccagtc acggcagtct taaaggttta ctttaaggta
     7861 acgcgcgtgt tgatccccgg atgataagtt cgcagtccat taaacgagag ccactgccaa
     7921 cgtgttgccc ctgcatttga tcaagcaata acagcatagc ttcccgaccg atttcgtaac
     7981 gcggctgcgc gatggttgtc agcggcggat cacaaaattg cgtcaggtcg atgttatcaa
     8041 aaccgattat ggaaaggtct tccgggactt tcagcccctg gcgttttgcc tgagaaagtg
     8101 cgccgagcgc catcacatcg ctatggcaga agactgcagt aggcggttgt ggaagatcaa
     8161 gcagctgttg cattgctttg cttccggctt cgaaggtgaa gtcgccacgg gcgatgtatt
     8221 gtggatcaac cataattccg cagcgacgca gcgcctgaac atagccttgc aggcggtagt
     8281 gacacagtgg catctcttcg ggaccggcta tacagccaat ccgtttatgc ccttgctcat
     8341 ataaataatt tactgcatca aatgcggcgg tcagattgtc gatatgaacg gtaggcagct
     8401 ccagttccgg tgcaaattcg ttcgccatca ccatcggcgg cagattacgc tgttcctcaa
     8461 tgctggcatc aaacggcagc cttgaaccca gcagcaacat gccatcaatt tgcttggtga
     8521 tgatcaaatc gataaaggtt ttttcctgct gattttgatg cgcacagtcg ccaatcagca
     8581 ccagatatcc gtgatttgcc gccgtaactt cgataccgcg aataatttcg ctaaagaagg
     8641 gatcgcagat atccgggaca atcaccagaa tggtgcggga ttcattacgc ttgacgttgc
     8701 gccccatagg ctgcggtaaa taacccactt cccgggccgc tttttcaacc cgattacggg
     8761 tggcctggga gactttatcg ggattcatta atgctcggga gacagtcgct gtagagactt
     8821 ttgccttgag ggcaacgtct ttcatggtcg cggcagtttc ctgcttcttc gctttcacac
     8881 tcactcctcg cctggcacgt caggcgtact acatccatgt ttacttcaca tcggcaacat
     8941 ttttagcaga tagcgcgtga aaacggttac agaattttca tgaaaagtgt gatgaatatt
     9001 gaatttttcg atccgcctcg catcgtaagc ggtttaaccc tcaatcggat caacatccag
     9061 tacccatttc accttgcggg aatccggtat tgtattgatg agcgccagcg taccactaat
     9121 gatgtgttgc aagcgcacgc gggaagggtg ctgcaacaat atctgccagc gccagcgacc
     9181 gccacgttta ggtgccagtg ccggaaccgg accgagaacc cacagtttgt cgtctgccag
     9241 cgggctggag aggatcagat tacgcagttg ttgcaggaac agcggcgcat gctgattgtt
     9301 atgatcttcc gcacgcacaa tcacatggct ggtccatggc ggcagttgca tcatccgtcg
     9361 ctcagccagc gcctgttcgg caaaggcatc gtagccttta tagagcaacg tttgcaacag
     9421 aggatgttcc ggatggtgcg tttgcagcac cacttcgcct tgtttacccg cacgcccggc
     9481 acgaccagcg acctgggtgt aaagctgagc gaaacgctct gccgagcgaa aatcggcaga
     9541 aaacagcgcg ccgtccacat ccagtaacgc caccagcgtc acatccggaa agtggtgacc
     9601 tttcgccagc atttgtgtgc cgatcaaaat cctcgcaccg ccgcgatgta cttccgccag
     9661 ttgctgttcc agcgcccctt tgcggctggt ggtatcgcgg tcgatacgag aaatgggcac
     9721 atcagggaac agcggcgcaa gcgtctgttc aagctgttcg gtgcccagcc ccacgggtac
     9781 caggtgcgtg gaaccgcagg aagggcactg gcgcggcacc ggacgctgac tgtcacagtg
     9841 gtgacagcgc aaatgctgtt gcgcctgatg cagcgtgtag tagtgatcgc aacgtgggca
     9901 ttcggcaatc cagccgcagt cgtggcacag tagcgcaggc gcaaagccac ggcggttaag
     9961 aaagaggata acctgattgt tggcctgtaa atgctggcgc atacgggtga ttaacgccgg
    10021 agccagacct gcctgcacct tctgaccttt taaatccagc acgtgttgaa ttgccggacg
    10081 cgcattcccc gcccggcgcg tcaggcgcag caggcggtat tttttctgct ggacgttgca
    10141 caacgtttcc agcgcgggcg ttgcggagcc aagaataatc gggatttgct cgctatgcgc
    10201 acgatacacc gccaggtcgc gggcatgata gcgccagcct tcctgctgct tgtaggaact
    10261 gtcgtgctct tcgtcaatga caatcacgcc gagattttta aacggcgtaa acagcgcgga
    10321 gcgggtgccg atcacaatcg ccgcctcgcc atttttcgct ttcagccacg ccgaaagacg
    10381 ctcgctgtcg ttcagaccgg aatgcagaac ttccacgggc gcattaaacc gttcacgaaa
    10441 acgggcgata gtttgcggtg tcaggccgat ttccggcacc atcaccagcg cctgtttgcc
    10501 ctgagcgagc acgttttcca gtacactgag ataaacctcc gttttaccgg aaccggtaac
    10561 gcccgccagc agccaggcag aaaaagtatc tgccgcgcta tgaattgcgc caacggcggt
    10621 ggcctgttcg gtattcaatc gcaaccgctc accagaaacg gcatagttcg ttcgccagtc
    10681 gctaaactct ggtgtttcac ttgctaaatc acacagacct tttttgcgca gcgcctgcaa
    10741 cgcggcatca ttaaattcga gcgtggcaac ctggtcgcgc cagattttgc cttgccgtaa
    10801 cgctgccagc gcctgttgtt gcttgggtga gcgtttcagg ctgttcagat ccaccgcctg
    10861 accttgttca gtggcaaacc agtaccacat cggcgcgttc gccgcaggcc gcccctggcg
    10921 tagtaaaatc ggcaaggcat gaaacagcac atcgccaatc ggatgatgat agtaatctgc
    10981 cgcccatagc agcaatcgcc agacggagtg agtaaacacc ggctcaccat ccagcacttc
    11041 gactaccgct tttagctcag tgagcggcag ttcgctggca tcgctaactg ataccacaat
    11101 cccgatgcgc tcctgctgtt tgccaaacgg cacgcgcacg cgacacccag ctttaaccgt
    11161 catgccttct ggcagcagat agtcaaaggt acgaggaagc ggaacgggca aggcaacgtg
    11221 ggcaacgggc atagcatcat cctgacttga aattcggtgg gttagtatac acattgccgt
    11281 agaagagtgc ggatcagttt gcatacgctg gttaatttct gtatgatttc gcgccttcgt
    11341 acgaaatgat cgtattgaag ctatactttt aacatcgcgt ggtgtctggc gttagggctg
    11401 gaagagcgac gcggccttaa accgaggttt tcccatgaaa aaagatattc acccgaaata
    11461 cgaagaaatt actgctagct gctcttgcgg taacgtaata aaaatccgct ccaccgttgg
    11521 tcatgacctg aacctcgacg tgtgcagcaa gtgccacccg ttcttcactg gcaaacagcg
    11581 tgatgttgct accggtggcc gtgttgaccg cttcaacaag cgtttcaaca tcccgggcag
    11641 caaataagac cggatttccg aaaaaagcgc cgcacggcgc tttttttgtg cctgaatttt
    11701 ggtcgtatca ccacgaaaac agcggccact ctttggcaac cgtggtgagt tgtggcgcgt
    11761 cgaaaacggc ttgtggtgaa actaccgttt cctcaagcgg aatattttta ccgtagcgtt
    11821 ctttgagctt cgcctggacc agcgggttgc tgagatcaaa ctctttcaac ttctgctgtt
    11881 cgcccacgcg cattcccagc gcgttctggt aaaccttcca caccacttcc gaacagtact
    11941 ggcgatcgtc gctccatgag aagctgaaat catacggttt accgagataa cgttttgccg
    12001 tttgcgtcag tttttgctgt tgttcaacac tcagtccgcc ttcaacgcgg cgaacaacgt
    12061 agttgccctt ttcaccatgg gcaatccact gcttgagcgg ggtgtatttc accgggccga
    12121 ccgcttcaaa aacgtagggc tttttgttac gcatcaccag cataccggtg tggctgtaat
    12181 cggaatgggt tgccagttgg atcgctttac tttgcgatga gcgagagatt tgaaagatga
    12241 tgtcgccagt ttgcggctgc caggcaaagg caggtacaga aaccagcagg ctgaggatca
    12301 gcagcctgtt tttcatagtt aaacgtccat gtataaaaag cggtgggtcg cagacaacgt
    12361 gctcgttgtt tatgccggat gcggcgtgaa cgccttatcc ggcctacaag ttcgtgcaaa
    12421 ttcaataaat tgcaatatga cgtaggcctg ataagcgtag tgcatcaggc gattccactc
    12481 cgcgccgctc ttttccgctt tagtattccc acgtctccgg gttaatcccc atctcacgca
    12541 tgatctcttt tgccgcttcc gggatttcgt cgctgcgctc tttacgcaga tcggcatcat
    12601 ccggcaaagg ttgcccggta aaggcatgca gaaacgcttc gcacagcagc tcgctgttgg
    12661 tagcgtgacg caggttgttc acctgacgac gcgtgcgttc atcggtgagg atttttaaca
    12721 ccttaagagg aatggaaacc gtaatctttt tgacttgttc actcttcttg ccgtgctcag
    12781 cgtatgggct gatatattcg ccgctccatt cagccatgag atacttaatc ctcttcgtca
    12841 ataaattgag accagacccc acaatttatg tgggtactga ccgtaaaccc gcatagttta
    12901 ccgtacaggc gttaccgtga catcgtgtaa tgcacctgtc ggcgtgataa tgcatataat
    12961 tttaacggct atttgggatt tgctcaatct atacgcaaag aagtttagat gtccagatgt
    13021 attgacgtcc attaacacaa tgtttactct ggtgcctgac atttcaccga caaagcccag
    13081 gaaacttcat cacatgacgc gtaaacaggc caccatcgca gtgcgtagcg ggttaaatga
    13141 cgacgaacag tatggttgcg ttgtccctcc catccatctt tccagcacct ataactttac
    13201 cggatttaat gaaccgcgcg cgcatgatta ctcgcgtcgc ggcaacccaa cgcgcgatgt
    13261 ggttcagcgt gcgctggcag aactggaagg cggtgctggc gcagtactta ctaataccgg
    13321 catgtccgcg attcacctgg taacgaccgt ctttttgaaa cctggcgatc tgctggttgc
    13381 gccgcacgac tgctacggcg gtagctatcg cctgttcgac agtctggcga aacgcggttg
    13441 ctatcgcgtg ttgtttgttg atcaaggcga tgaacaggca ttacgggcag cgctggcaga
    13501 aaaacccaaa ctggtactgg tagaaagccc aagtaatcca ttgttacgcg tcgtggatat
    13561 tgcgaaaatc tgccatctgg caagggaagt cggggcggtg agcgtggtgg ataacacctt
    13621 cttaagcccg gcattacaaa atccgctggc attgggtgcc gatctggtgt tgcattcatg
    13681 tacgaaatat ctgaacggtc actctgacgt ggtggctggc gtggtgattg ctaaagaccc
    13741 ggacgttgtc actgaacttg cctggtgggc aaacaatatt ggcgtgacgg gcggcgcgtt
    13801 tgacagctat ctgctgctac gcgggttgcg aacgctggtg ccgcgtatgg agctggcgca
    13861 gcgcaacgcg caggcgattg tgaaatatct gcaaacccag ccgttggtga aaaaactgta
    13921 tcacccgtcg ctgccggaaa atcaggggca tgaaattgcc gcgcgtcagc aaaaaggctt
    13981 tggcgcaatg ttgagttttg aactggatgg cgatgagcag acgctgtgtc gtttcctggg
    14041 cgggctgtcg ttgtttacgc tggcggaatc attaggggga gtggaaagtt taatctctca
    14101 cgccgcaacc atgacacatg caggcatggc accagaagcg cgtgctgccg ccgggatctc
    14161 cgagacgctg ctgcgtatct ccaccggtat tgaagatggc gaagatttaa ttgccgacct
    14221 ggaaaatggc ttccgggctg caaacaaggg gtaaaaatga gtgtgattgc gcaggcaggg
    14281 gcgaaaggtc gtcagctgca taaatttggt ggcagtagtc tggctgatgt gaagtgttat
    14341 ttgcgtgtcg cgggcattat ggcggagttc tctcagcctg acgatatgat ggtggtttcc
    14401 gccgccggta gcaccactaa ccagttgatt aactggttga aactaagcca gaccgatcgt
    14461 ctctctgcgc atcaggttca acaaacgctg cgtcgctatc agtgcgatct gattagcggt
    14521 ctgctacccg ctgaagaagc cgatagcctc attagcgctt ttgtcagcga tcttgagcgt
    14581 ctggcggcgt tgctcgacag tggtattaac gatgcggtgt atgcggaagt ggtgggccac
    14641 ggtgaagtgt ggtcggcgcg cctgatgtct gcggtactta atcaacaagg gctgccagcg
    14701 gcctggcttg atgcccgtga gtttttacgc gctgaacgcg ctgcgcaacc gcaggttgat
    14761 gaagggcttt cttacccgtt gctgcaacag ctactggtac aacatccgag caaacgtctg
    14821 gtggtgaccg ggtttatcag ccgtaacaac gccggtgaaa cggtgttgct ggggcgtaac
    14881 ggttccgact attccgcgac acaaatcggt gcgctggcgg gtgtttctcg tgtaaccatc
    14941 tggagcgacg tcgccggggt atacagtgcc gacccgcgta aagtgaaaga tgcctgcctg
    15001 ctgccgttgc tgcgtctgga tgaggccagc gaactggcgc gtctggcggc tcccgttctt
    15061 cacgcccgta ctttacagcc agtttctggc agcgaaatcg acctgcagct gcgctgtagc
    15121 tacacgccgg atcaaggttc cacgcgcatt gaacgcgtgc tggcctccgg tactggtgcg
    15181 cgtattgtca ccagccacga tgatgtctgt ttgattgagt ttcaggtgcc cgccagtcag
    15241 gatttcaaac tggcgcataa agagatcgac cagatcctga aacgtgccca ggtgcgtcca
    15301 ctggcggttg gcgtgcataa cgatcgccag ttgttgcaat tttgctacac ctcagaagtg
    15361 gccgacagtg cgctgaaaat cctcgacgaa gcgggattac ctggagaact gcgcctgcgt
    15421 caggggctgg cgctggtggc gatggtcggg gcgggtgtca cccgtaaccc gctgcattgc
    15481 caccgcttct ggcagcaact gaaaggccag ccggtcgagt ttacgtggca gtccgatgac
    15541 ggcatcagtc tggtggcagt actgcgcacc ggcccgaccg aaagcctgat tcaggggctg
    15601 catcagtccg tcttccgcgc agaaaaacgc atcggcctgg tattgttcgg caaaggcaat
    15661 atcggctctc gctggctgga actgttcgcc cgcgagcaga gcacgctttc ggcgcgtacc
    15721 ggctttgaat ttgtgctggc aggcgtggtg gacagccgcc gcagtctgtt gagttatgac
    15781 gggctggacg ccagccgcgc gttagccttc ttcaacgatg aagcggtcga gcaggatgaa
    15841 gagtcgcttt tcctgtggat gcgcgcccat ccgtatgatg atttagtggt gttggacgtt
    15901 accgccagcc agcagcttgc tgatcagtat ctcgatttcg ccagccacgg tttccacgtc
    15961 atcagtgcca acaaactggc gggagcaagc gacagcaata aatatcgcca gatccacgac
    16021 gccttcgaaa aaaccgggcg tcactggcta tacaacgcca ccgttggtgc gggcttgccg
    16081 atcaaccata ccgtgcgcga tctgatcgac agcggcgata ccattttgtc gatcagtggg
    16141 atcttctccg gcacgctctc ctggctgttc ctgcaattcg acggtagcgt gccgtttacc
    16201 gagctggtgg atcaggcgtg gcagcagggc ttaaccgagc ctgatccgcg tgacgacctc
    16261 tccggcaaag acgtaatgcg caagctggtg attctggcgc gtgaagcggg ttacaacatt
    16321 gaaccggacc aggtacgtgt ggaatcgctg gtgcctgctc attgcgaagg cggcagcatc
    16381 gaccatttct ttgaaaatgg cgatgaactt aacgagcaga tggtgcaacg gctggaagcg
    16441 gcccgcgaaa tggggctggt gctgcgctac gtggcgcgtt tcgatgccaa cggcaaagcg
    16501 cgtgtgggcg tggaggcggt gcgtgaagac tatccgctgg catcactgct gccatgcgat
    16561 aacgtctttg ccatcgaaag ccgctggtac cgcgataatc cgctggtgat ccgtgggcct
    16621 ggcgctgggc gcgacgtcac cgccggggcg attcagtcgg atatcaaccg gctggcacag
    16681 ttgttgtaat ttcagaaa
//
LOCUS       DAZXUK010000068        16585 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZXUK010000068 DAZXUK010000000
VERSION     DAZXUK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16585
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.068"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(139..3654)
                     /locus_tag="ACH3L8_001771"
     CDS             complement(139..3654)
                     /locus_tag="ACH3L8_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFT4084918.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="ACH3L8_001772"
     CDS             complement(4107..5369)
                     /locus_tag="ACH3L8_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4084919.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="ACH3L8_001773"
     tRNA            complement(5567..5642)
                     /locus_tag="ACH3L8_001773"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="ACH3L8_001774"
     CDS             complement(5748..6455)
                     /locus_tag="ACH3L8_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFT4084920.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6608..6691
                     /gene="yqgH"
                     /locus_tag="ACH3L8_001775"
     CDS             6608..6691
                     /gene="yqgH"
                     /locus_tag="ACH3L8_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFT4084921.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="ACH3L8_001776"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="ACH3L8_001776"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFT4084922.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="ACH3L8_001777"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="ACH3L8_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFT4084923.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="ACH3L8_001778"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="ACH3L8_001778"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFT4084924.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="ACH3L8_001779"
     CDS             complement(11639..11914)
                     /locus_tag="ACH3L8_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFT4084925.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="ACH3L8_001780"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="ACH3L8_001780"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFT4084926.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="ACH3L8_001781"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="ACH3L8_001781"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFT4084927.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="ACH3L8_001782"
     CDS             13874..14200
                     /locus_tag="ACH3L8_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFT4084928.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="ACH3L8_001783"
     CDS             14384..15103
                     /locus_tag="ACH3L8_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFT4084929.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="ACH3L8_001784"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="ACH3L8_001784"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFT4084930.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..>16585
                     /locus_tag="ACH3L8_001785"
     CDS             16442..>16585
                     /locus_tag="ACH3L8_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFT4084931.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAI"
BASE COUNT         4155 a         4098 c         4128 g         4204 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatc
//
LOCUS       DAZXUK010000069        16521 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZXUK010000069 DAZXUK010000000
VERSION     DAZXUK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16521
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.069"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            138..290
                     /gene="mokC"
                     /locus_tag="ACH3L8_001786"
     CDS             138..290
                     /gene="mokC"
                     /locus_tag="ACH3L8_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFT4084932.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(394..1524)
                     /gene="dnaJ"
                     /locus_tag="ACH3L8_001787"
     CDS             complement(394..1524)
                     /gene="dnaJ"
                     /locus_tag="ACH3L8_001787"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFT4084933.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1613..3529)
                     /gene="dnaK"
                     /locus_tag="ACH3L8_001788"
     CDS             complement(1613..3529)
                     /gene="dnaK"
                     /locus_tag="ACH3L8_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFT4084934.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3906..4310
                     /locus_tag="ACH3L8_001789"
     CDS             3906..4310
                     /locus_tag="ACH3L8_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFT4084935.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4336..5049
                     /gene="msyB"
                     /locus_tag="ACH3L8_001790"
     CDS             4336..5049
                     /gene="msyB"
                     /locus_tag="ACH3L8_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFT4084936.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5198..5764
                     /gene="satP"
                     /locus_tag="ACH3L8_001791"
     CDS             5198..5764
                     /gene="satP"
                     /locus_tag="ACH3L8_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFT4084937.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5799..6386)
                     /gene="mog"
                     /locus_tag="ACH3L8_001792"
     CDS             complement(5799..6386)
                     /gene="mog"
                     /locus_tag="ACH3L8_001792"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFT4084938.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6501..7454)
                     /gene="tal"
                     /locus_tag="ACH3L8_001793"
     CDS             complement(6501..7454)
                     /gene="tal"
                     /locus_tag="ACH3L8_001793"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFT4084939.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7733..9163
                     /locus_tag="ACH3L8_001794"
                     /pseudo
     CDS             7733..9163
                     /locus_tag="ACH3L8_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            9233..10009
                     /gene="yaaA"
                     /locus_tag="ACH3L8_001795"
     CDS             9233..10009
                     /gene="yaaA"
                     /locus_tag="ACH3L8_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFT4084940.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(10162..10458)
                     /locus_tag="ACH3L8_001796"
     CDS             complement(10162..10458)
                     /locus_tag="ACH3L8_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFT4084941.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10672..11958)
                     /gene="thrC"
                     /locus_tag="ACH3L8_001797"
     CDS             complement(10672..11958)
                     /gene="thrC"
                     /locus_tag="ACH3L8_001797"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFT4084942.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(11959..12891)
                     /gene="thrB"
                     /locus_tag="ACH3L8_001798"
     CDS             complement(11959..12891)
                     /gene="thrB"
                     /locus_tag="ACH3L8_001798"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFT4084943.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12893..15355)
                     /gene="thrA"
                     /locus_tag="ACH3L8_001799"
     CDS             complement(12893..15355)
                     /gene="thrA"
                     /locus_tag="ACH3L8_001799"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFT4084944.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKNISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSLSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15381..15500)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15436..15501)
                     /gene="thrL"
                     /locus_tag="ACH3L8_001800"
     CDS             complement(15436..15501)
                     /gene="thrL"
                     /locus_tag="ACH3L8_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFT4084945.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15715..16401)
                     /locus_tag="ACH3L8_001801"
     CDS             complement(15715..16401)
                     /locus_tag="ACH3L8_001801"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFT4084946.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3818 a         4411 c         4094 g         4198 t
ORIGIN      
        1 tgtctgtctc aggcattagc tgaacggcag atagagaaaa gccccgagtg atattttacc
       61 atcaacccga ggcctcctat atgctgaaca catgtagagt gcctcttact gaccgtaagg
      121 tcaaggagaa gagagcaatg aagcagcata aggcgatgat tgtcgccctg atcgtcatct
      181 gtatcaccgc cgtagtggcg gcgctggtaa cgagaaaaga cctctgtgag gttcacatcc
      241 gaactggcca gacggaggtt gctgttttca cggcttacga atccgagtaa gagcaacggc
      301 ggggagtgat ccccgccact ttacaggtgc tcgcatatct tcaacgcacc ctatttttac
      361 ccaggcctgc ccacgggcag gcttttgggg aggttagcgg gtcaggtcgt caaaaaactt
      421 cttcacacca tcaaagaagc tctttgagcg cgggctgttg tgctcgccgg ttgggccacc
      481 gaagctttct tgcagctctt gcagcagctg tttctgcttt tcgttcaggc ctaccggtgt
      541 ttcgacgaca acgcggcaca gcaaatcacc ctgtgcgcca ccgcggacag acttgacgcc
      601 tttaccgcgc atacggaata gcttaccggt ctgggtttcg ccaggcactt tcagtttgac
      661 gcgaccatca agggtcggta cttcgatttc gccacccagc gccgccatag cgaagttgat
      721 cgggacttcg caatacaggt tgttgccttc acgctcgaaa atcgggtgct gtttaacctg
      781 aacctgaacg tacagatcgc ctgccggtgc gccatgctcg cccgcttcac cttcgcccgc
      841 aagacggatg cggtctccag tgtccacccc tgccgggatt ttaacggaca gcgttttgct
      901 gcgctcaaca cgaccatgac catgacattt gttgcacgga tctttgatca gcgtaccgcg
      961 gccctgacag tgtggacagg tctgctgtac agcgaagaat ccctggcgca tctgcacctg
     1021 accagaacca tgacaggtcg gacaggtctg cggctgtgta cctggttttg caccgctacc
     1081 gtggcaaacg tcacactctt ccagagtcgg aatgcggatc tctttggtca cgccacgtac
     1141 agcttcttcg agggtgagct ccatgttata gcgtaaatca gcaccgcgcg ccgcacgttg
     1201 acgaccacgt ccgccgccaa aaatatcgcc gaaaacgtca ccaaaaatat cgctgaagtc
     1261 tgcgccgccg ccaaaaccac cgccgcccat gcctccttgc tcaaacgcag catgaccata
     1321 ctgatcgtat gccgcacgtt tttgcgagtc ggtcagaact tcataagctt ccttgatctc
     1381 tttaaatttc gcctcggcct ctttgtcacc ctggttacgg tccgggtggt atttcatggc
     1441 caggcgtttg taggcctttc tgatttcacg ctcttccgct gttttggaaa cgcctaaaat
     1501 ctcgtaataa tcttgcttag ccatcttttt taaattgccc ctagatgaat gcacgggcgg
     1561 agaggaaatt ccccttcgcc cgtgtcagta taattacccg tttatagggc gattattttt
     1621 tgtctttgac ttcttcaaat tcagcgtcga caacatcgtc atctttcgcg ttgtttgcag
     1681 aagcatcagc accggcagtc tgctgctggg catgttgctg ctgggcgatt tccatcagtt
     1741 tctgggaaac ctgtgccagt tcctgcattt tcgcttcgat agcggctttg tcttcacctt
     1801 tcagagcagt ttccagtgca gtcagcgcag actcgatagc agttttgtcg tcagccggca
     1861 gtttgtcgcc tgcttcttca acctgcttac gggtgctgtg cagcagatgg tcgccctggt
     1921 tgcgagtctg taccagctct tcaaacttac ggtcagcttc ggcgttagct tctgcgtcgc
     1981 gtaccatttt ctggatttca tcttcgttca gaccagaaga agccttgatg gtgatcttct
     2041 gctctttacc gctgttttta tctttcgcgg aaacgtgcag gataccgtca gcatcgatat
     2101 cgaaggtaac ttcgatctgc ggcatgccgc gcggtgccgg gttgatacca tctaggttga
     2161 actgacccag agatttgtta tcagccgcac gtttacgttc accctgcagc acatggatgg
     2221 ttaccgcaga ctggttgtct tcagcggtag agaacacctg gctgtgcttg gtcgggatag
     2281 tggtgttttt cgcgatcagc gtcgtcatca caccgcccat ggtttcgata cccagagaca
     2341 gcggggtaac gtccagcagc agtacgtctt ttacgtcacc agtcagaaca ccaccctgaa
     2401 cagcagcacc gattgctaca gcttcgtccg ggttaacgtc tttacgcggc tctttaccaa
     2461 agaactcagc aactttcttc tgaaccattg gcatacgagt ctgaccacca acgaggataa
     2521 cgtcgtcgat atcagatacg gacaggccag catcctgcag tgcaactttc agcggctcaa
     2581 tggaacggtt taccagatct tcaaccaggc tttccagttt cgcacgagtc actttgatgt
     2641 tcatgtgttt cggaccggtc gcgtctgcag tgatgtatgg caggttaacg tcggtctgct
     2701 gagcggaaga cagttcgatt ttcgcttttt ctgccgcttc tttcaggcgc tgcattgcca
     2761 gcggatcgtt gcgcaggtca atgccctgat ctttcttgaa ttcttcaacc agatagttga
     2821 tcagacggct gtcgaagtct tcaccaccca ggtgggtatc accgttggtt gccagaactt
     2881 cgaaggtttt ttcgccgtca acttcgtcga tttcgataat agaaatatcg aaagtaccac
     2941 cccccaggtc ataaaccgcg atagtacggt tgccagtgcc tttgtccaga ccgtaagcca
     3001 gcgcagctgc ggtcggttcg ttgatgatac gttttacttc cagaccagcg atacggcctg
     3061 cgtctttggt tgcctgacgc tgagcatcgt taaagtatgc cggtacggtg ataacagctt
     3121 cagttaccgg ttcacccagg taatcttcag cggttttctt catttttttc agcacttcag
     3181 cagaaatctg cggcggtgcc attttctggc ctttaacttc gacccatgcg tcgccgttat
     3241 cagcagcaat aattttgaac ggcatgatgg aaacatcacg ctgtacttct tcgtcctgga
     3301 agcggcggcc aatcaggcgt ttaatcgcaa acagggtgtt ttgcgggttc gtcactgcct
     3361 gacgtttagc cggctgacca accagagttt caccatcctg ggtataggca atgatagaag
     3421 gcgtggtgcg atcgccttcg gcgttctcca gcacgcgagg aatggtgcca tccataatcg
     3481 ctacacaaga gttggtagta cccaggtcga taccaattat tttacccatc taaacgtctc
     3541 cactatatat tcggtcatca tgtggttgtg agtctgtaat aggggcgaaa cgtctggttt
     3601 caactgccca atttcatttt tttgcagact cactcactgc ggttgactac taaatggggt
     3661 cgtaacccac gtcatcaagg gggagatgca aaaatttttt gtgcttttgc gcagaaattt
     3721 cgcgatatgg caggacaaga cgtaaaattg acagcaattt tattcatata aagaatgaat
     3781 cgcagccaag gcgttcatca aaaaattgta ataaaaagaa aagattacgt gcctgaatct
     3841 tctctttatc agcagtaaac tagtgggtat tcatccccct acctcttccc actaagagaa
     3901 tccttatgaa atccgttttt acgatttccg ccagcctggc gattagcctg atgctgtgct
     3961 gcacggcgca ggcaaacgac cataaactcc tcggcgtcat tgcaatgccg cgtaacgaaa
     4021 ccaacgatct ggcgctgaaa cttcctgttt gtcgcattgt gaaacgcata caactctctg
     4081 ccgaccatgg cgatttacag ttaagtggtg catcggttta tttcaaagcc gcccgtagcg
     4141 ccagtcagag cctgaatatt ccttcagaaa taaaagaagg gcaaaccact gactggatca
     4201 acattaacag cgataacgac aataaacgct gcgtctcaaa aatcaccttt tccggtcata
     4261 cggtgaactc atcggatatg gccacgctga aaattatcgg cgacgactaa cgccagaata
     4321 tagaagccac aaaaaatgaa tgttaattac ctgaatgatt cagatctgga ttttcttcag
     4381 cattgtagtg aggaacagtt ggcaaatttc gcccgattgc tcacccataa tgaaaaaggc
     4441 aaaactcgcc tctccagcat actgatgcgc aacgaactgt ttaaatcgat ggaagggcat
     4501 cccgagcaac atcgccgcaa ctggcagctg attgccggag aattacagca ttttggtggc
     4561 gatagtatcg ccaacaaact gcgcggacac ggtaaattgt atcgggccat tttgctcgat
     4621 gtttcaaagc gattgaagct gaaagccgac aaagagatgt ctacgtttga aattgagcaa
     4681 cagttactgg aacaatttct gcgtaatacc tggaagaaaa tggacgagga acataagcag
     4741 gagtttctgc acgcggtcga tgccagggtg aatgagctgg aagagctgct gccgctgctg
     4801 atgaaagaca aattattggc aaaaggtgtg tcgcatttgc tttccagcca actgacccgc
     4861 attttacgca cccacgcagc aatgagcgta cttgggcatg gtttgctgcg cggcgcgggg
     4921 ctgggaggcc ctgtgggcgc agcattaaat ggggttaaag cggtcagcgg cagcacctat
     4981 cgcgtgacga ttccagccgt actgcaaatc gcctgcctgc gccggatggt tagcgccact
     5041 caggtctgat accggaaacc ggggaaaatt ttcattttcc cctcaaaaga tcgtagacac
     5101 tgccccactg gctgattatt atgccgcgcc ctgaaaacac tacagttatt cagggaaatt
     5161 atttcaccat tcattcgatg atgatttttg aggaattatg ggcaacacta agttggctaa
     5221 tccggcaccg ctgggcctga tgggcttcgg catgaccacc attctgctta acctgcacaa
     5281 cgtgggttat ttcgctctgg acggtattat tcttgccatg ggcattttct acggcggcat
     5341 cgcgcaaatt tttgctggtc tgctggagta caaaaaaggc aacactttcg gtttaaccgc
     5401 attcacctct tacggttctt tctggctgac gctggttgcg attctgctga tgccgaaact
     5461 gggtctgacc gatgcgccaa atgcacagtt ccttggtgtc tacctgggtc tgtggggcgt
     5521 atttacgctg tttatgttct tcggcactct gaaaggcgcg cgcgtcctgc aattcgtttt
     5581 ctttagcctg accgtgctgt ttgccctgct ggcgatcggt aacattgccg gtaatgccgc
     5641 gatcatccac tttgccggct ggattggtct gatctgcggt gccagcgcaa tctatctggc
     5701 gatgggtgaa gtgctgaacg agcagtttgg tcgcaccgtt ctgccgattg gtgaatccca
     5761 ctaattgtct tgagatcccc ccgctcgggg ggattttttt attcgctaac gtcgcgtctt
     5821 gcactcttcg gtctgaatgc agcaaccact tctggtgccg tttcaacgta tggcccttcc
     5881 agcaactgaa tgcagtacgg tacgctggca aaaataccat gcaccacaac gttaccctca
     5941 gcgtccttca caccttccag cgtctcttta atagacttcg gctgaccggg taagttaagg
     6001 atcagcgcct gtttgcgaat cacgcccacc tgacgcgaaa ggatcgcagt tggtacaaaa
     6061 tgcaggctga tctggcgcat ctgttcacca aagccaggca tctcgcggtc cgctactgcc
     6121 agcgtcgcat cgggcgttac gtcacgacgc gccgggccag ttccgcccgt ggtgagcacc
     6181 agatggcaac tcatttcatc caccagctca cacaacgttt gctcgatgat cgcctgctca
     6241 tcggggatta agcgggtttc cagttcaaac ggcgtggtta gcgccgatgt cagccattct
     6301 tccagcgctg gaatgccttt atcctgataa acgccgctgg atgcgcgatc agagatggaa
     6361 actaagccaa tacgtaaagt attcatcaag atttttccgt ttaaacaatc tcgttaaaca
     6421 gaatgataca ctgcgaaggg agtgacagac aggcttcaga agaggtagcg tgaccgactt
     6481 cccggtcacg ctaagaatga ttacagcaga tcgccgatca ttttttccag tttttcctgg
     6541 tcaatagcaa acttacggat accttccgcc agtttatcta ctgccattgg atcctggttg
     6601 tgctgccaca ggaactcgga ctcagtgata cgcgccggac gcgctttcac ttcgccggtg
     6661 taagacagtt tacgttcgat agccccttcg ctctccgcca gctctttcag cagtgccggt
     6721 gcgatggtca gacggtcaca gcctgccagt tccagaattt cgccgatgtt acggaagctt
     6781 gcgcccataa ccacggtttc ataaccgtgc tctttgtagt actggtagat ttcagataca
     6841 gaaaccacgc ccggatcttc tgccggagcg tactctttct tatcggtatt cgctttgtac
     6901 cagtcaagaa tacggccaac aaacggcgag atcaggaaca cgcccgcctc cgcacaagca
     6961 cgagcctgag cgaaggagaa cagcagggtc aggttacagt tgataccttc tttttccagc
     7021 tgttctgcag cacggatacc ctgccaggta gaagccagtt tgatcagaat acgatcgttg
     7081 ctaatacctg catcgttgta gagtttgatc aggcgttttg ctttcgcaat tgacgcttca
     7141 gtgtcatagg aaagacgcgc atcaacttca gttgagatac ggcccggaac cagtttcagg
     7201 atttccagac caatatttac tgccagtttg tcggtcgcgt ccacgatctg ctgcgcgcga
     7261 tcgttgctct gctgtttcgc ccaggcgaca gcatcatcaa tcaacttacg gtattccgga
     7321 atctgcgctg cgttaagaat gagagaaggg ttggttgtgg catcctgcgg ttgatacagc
     7381 ttcattgccg cgatgtcccc agtgtcggcc actacggtgg tgtactgacg aagggaggtc
     7441 aatttgtccg tcatgatagt atttctcttt aaacagcttg ttagggggat gtaaccggtc
     7501 tgccctgatg atatcacgac gcaacgtcag cgcaaccgcc gacaagacgg ttatcgccag
     7561 gcgagactgt ttcggatttc tgacactgcg ttgatatcgc ccaccatttt tatacaaaac
     7621 ctcatgtatg ctacgcagaa gttatcaagt acctcgtagc gtatatactt cttaaacaat
     7681 tggtaacgtt tacacaggaa agtcatcgcg accggcaata agagggatat gcatgccaga
     7741 ttttttctcc tttattaaca gcgtcctttg gggatcggta atgatttacc tgctcttcgg
     7801 cgcaggttgt tggttcactt ttcgcaccgg atttgtgcag tttcgctaca tccgccagtt
     7861 tggcaaaagt cttaaaaata gcattcatcc acagccaggc ggtttaacct catttcagtc
     7921 attgtgtacc agtcttgcgg cgcgcgtggg tagcggcaac ctggccggcg ttgcgctggc
     7981 aattaccgcc ggtggaccag gtgccgtctt ttggatgtgg gttgctgcgt ttatcggcat
     8041 ggcgacctcg tttgccgaat gttcccttgc acaactttat aaagaacgtg acgtcaatgg
     8101 gcagtttcgt ggcggaccgg catggtatat ggcgcgcggg ctggggatgc gctggatggg
     8161 cgttctgttc gccgtctttt tgctcatcgc ctacggcata attttcagcg gagttcaggc
     8221 gaacgccgtt gcccgagccc tgagtttttc ttttgatttt cctccgctgg tgacgggcat
     8281 tattctcgct gtctttgctc tgctggcaat cactcgtggt cttcatggcg tcgcccggct
     8341 catgcagggg tttgtcccgt tgatggcgat aatctgggta ctgaccagcc tggtgatttg
     8401 cgtaataaat atcgggcaac ttccccacgt catttggtct atttttgaga gtgcttttgg
     8461 ctggcaggaa gcggcaggcg gcgcggcggg atatacctta agccaggcga ttactaacgg
     8521 ttttcagcgc agtatgtttt ccaatgaggt gggaatggga tcgacgccaa acgcggcagc
     8581 ggcagctgca tcctggcctc cgcatccggc agcacaaggg attgtccaga tgattggcat
     8641 ttttatcgac accctggtca tctgtacggc aagcgccatg ctgatattac tggcgggtaa
     8701 cggcacaacc tacatgccgc tggaaggtat tcagcttatc cagaaggcga tgcgggtgct
     8761 aatgggttcc tagggtgctg aatttgttac cctcgtggtt attctgtttg ccttcagctc
     8821 catcgttgcc aactacattt atgccgaaaa caatctcttc tttttacgcc tgaacaaccc
     8881 taaagcgatc tggtgtttgc ggatctgcac cttcgcaacg gtcatcggcg gcaccttgct
     8941 aagccttccg ctgatgtggc aactggcaga tatcataatg gcctgcatgg ctattaccaa
     9001 tttgaccgcc attttactgc tctcgcctgt ggttcatacc attgccagtg attatctacg
     9061 ccagcgtaaa ctcggcgtgc gcccggtgtt tgatccgttg cgttatccgg atatcggtcg
     9121 ccagctttct ccggacgcgt gggatgatgt ttcgcaggag taatcacaac tatcgatcaa
     9181 ctcattctct ttttttgcta aagtcggcat aaatttcctg caaggactgg atatgctgat
     9241 tcttatttca cctgcgaaaa cgcttgatta ccaaagcccg ttgaccacca cgcgctatac
     9301 gctgccggag ctgttagaca attcccagca gttgatccat gaagcgcgga aactgacgcc
     9361 tccgcagatt agcacgctga tgcgcatcag cgacaaactg gcgggtatca acgccgctcg
     9421 ctttcatgac tggcagccag atttcacgcc ggcgaatgcc cgccaggcga ttctggcgtt
     9481 taaaggtgat gtctacaccg gcttgcaggc cgaaaccttc agcgaagacg atttcgattt
     9541 tgcccaacag catttgcgaa tgctttccgg cttgtatggc gtactgcgcc cgctcgattt
     9601 aatgcagcct tatcgtctgg aaatggggat ccgtcttgag aatgcccgag ggaaagatct
     9661 gtatcaattc tggggagata tcatcaccaa caagctgaac gaggcgctcg cagcacaagg
     9721 cgataatgtg gtgattaatc tggcgtcaga tgagtatttt aaatcggtga agccgaagaa
     9781 attgaatgcc gagattatca agccggtgtt cctcgatgag aagaacggca aatttaagat
     9841 catcagcttc tacgctaaga aagcacgcgg tctgatgagt cgtttcatta ttgaaaatcg
     9901 gctgaccaaa ccagagcaac tgactggttt taatagcgaa ggttacttct ttgatgaaga
     9961 ttcctccagc aatggcgaac tggtgtttaa acgctacgag cagcgttaag tttgtagtca
    10021 atgccggatg cggcgtgaac gccttatccg gcctacaaaa gcatgcagat tcaatatatt
    10081 gcagaattaa cgtaggcctg ataagacgcg ccagcgtcgc atcaggcgct gaatgccgga
    10141 ttgttacccg gcatttgtca tttagcggtg atgtttgcct ggaccatgac cgccgtgatg
    10201 atcatgagga gctttcttat ggtggcgcgg cggtggcggc ggtccgtgtg ggtgccagcg
    10261 attgcctcgc cattcataat gttgtttcca ccagccgtgg tcgcgccagt gacctccatc
    10321 ccagtaatag ccacgattat cacgatcgcc tatctgtaat tttactgacg ggactaacgt
    10381 aatttccgca gcctgtgctg ccatgggagc gaccagaacc agggaaagtg cgagtacgat
    10441 agattgcatc tttttcacag gttactcctt ttatcattgc ccgttatggg tcgatatccg
    10501 gagattacgc gatgagaacg acggttgtta ttctccgcaa tcctaatctc taagttaccg
    10561 catttattga taatttctcc tttttccctg tcgtttttct ccataatttc ttcataaaaa
    10621 agccgggctg cataaaagca aacccggcct gattgagata atgaatagat tttactgatg
    10681 attcatcatc aatttacgca acgcagaaaa atcggcgggc agattatgtg aaagcaaggg
    10741 taaatcagca cgttctgcca gctcttttgg cagatccaac gtttcaccga gaatcgcttc
    10801 cacgctctct ttaaatttcg ccggatgcgc ggtgccgagg aacaagccat attcgcctgg
    10861 attcaactgg tcacgcagcg cacgatacgc tacggcagcg tgcggctccg aggtgtagcc
    10921 cagttctttt aactcacgca ttgtctgttg cgtggtttca tcatccacgg ctgcataacc
    10981 cagctctttc agttgccaga ttttgcggcg gaacaactct tccacacgcg gccagttgtt
    11041 cggctgactc acgtccatcg cgttggataa cgtcgcctga gtcgctttgg gtgaccactg
    11101 accgtcgtgc aggaaacgtg gcacggtatc gttcacgttg gtcgcagcaa taaaacgttt
    11161 caccggcaga ccgaatgact tcgccagcag acccgccgtc aaatcgccga agtttccgct
    11221 tggcaccgag acaaccagct ggttgcgcgc ctcctgcggc agctgcgcaa cagcttcaaa
    11281 gtagtagcaa atctgcgcca gcaaacggct gatgttaatc gagttagccg agtttaaccc
    11341 cagcgccact ttcagttctt catcatcaaa cgcctgcttc accagcgcct gacaggcatc
    11401 gaaatcgccg tcgatggcaa cagtttcgat attgccgccc aacgtacaga acagtttttc
    11461 ttgcagtgga ctgattttgc ctcgtggata gaggataacc actttcacat tcggcaaacc
    11521 gtagaaagca tgagccactg cagctccggt atcaccggag gtcgcggtca gaatggtcac
    11581 tggcttatca tccgcaatat gggtcagcat ttgtgccata aagcgaccgc cgaaatcttt
    11641 aaatgccagc gttggcccgt ggaacaattc cagacaaccg acatcgcttt caacattggc
    11701 gaccggagcc gggaaggcaa acgccgcgcg cacgcgctct tccaggattt cctgtgggat
    11761 ttcatcacca ataaacgccg agaggatctt cgcactgcgg gtgacaaaat ccagcttcag
    11821 catctcatca atttcagtca ggctgaattc cggcaggtcg tgcggaaaaa acagcccctg
    11881 atttttgccc aacccctggg ttacggcttg cgcaaagctg acctgctcgt tgtgatcttt
    11941 cagattgtag agtttcattt agttttccag tactcgtgcg cccgccgtat ccagccggca
    12001 aatatgaaca aaaccttcct gattttgcag gtagttctta cccaaccagt cggcaacgcg
    12061 ctgggcggta tccggcttgt cacatagagc gaacaaggtc gggccggagc cggagatacc
    12121 gctcgctacc gcgccgattt ccgcgaccgc ctgccgcgcc tgccggaagc caggcagtaa
    12181 ccgttcacgg tagggttcag cgataacatc tttcatcagc ttcgcggcaa gctcaggctg
    12241 acgggaatag caggcgtgaa tgaagccagc cagatgtcgc ccgtgcgcaa tgcaatcctg
    12301 gcggcgatac tgcgccggta aaatagcccg ggcttctgcc gtcgagactt taattcccgg
    12361 atacgccagc acccacagcc actcatcaaa ccctggcact tgctggctga tgatgtcgtt
    12421 ttcttcgatc atcaactgca taccaccaag aaaacacggt gccacgttgt cgtaatgaat
    12481 gctgccggag atacgtcctt ccagctcgcc catcaaagcc agcaaacgag tgtcattaag
    12541 cggcttgccg caatgttcat tcatcgccat cagcgccgcg accaccgaac aggcgctgga
    12601 gcctaagccc gaaccgatcg gcatattctt ttccagagtc atcgccactg gaatttgctt
    12661 gccaagctcc tggcaaaaac gctcccagca ctgataaacg atattttccc gtggttctga
    12721 cggcagctta tcggcaaagc gtccgagttt gttgagactg aatgtctctg ccgcctcaac
    12781 cgtgactaca tctccgagca atgcaccatc aacgggtgcc accgccgccc cgagcacatc
    12841 aaacccgacg ctcatattgg cactggaagc cggggcataa actttaacca tgtcagactc
    12901 ctaacttcca tgagagggta cgtagcagat cggcaaagac accggcagcg gtaacgtcat
    12961 tgcccgcacc atatccgcgc agcaccaacg gcagcggctg ataatagtgg ctataaaagg
    13021 ccagggcgtt ttcgccattt ttcactttga acagcggatc attcccatcc acttcggcaa
    13081 tcttcacgcg gcagacacca tcttcatcaa tattgccaac atagcgcaaa acttttcctt
    13141 catcacgggc cttcgccacg cgcgcggcaa agagatcgtc gagctgtgac agattcgcca
    13201 taaaagcggc aacatcaccc tcggcgttaa actctgcggg cagcacaggt tcaatttcaa
    13261 tatccgccag ctccagttca cgtcccgttt cacgagcgag aatcaatagt ttacgcgcca
    13321 catccatacc agaaagatca tctcgcgggt ccggttcggt ataacccatt tcccgcgcca
    13381 gcgtggtcgc ctcggagagg ctcatgcctt cgtctaactt gccgaagata taagaaagcg
    13441 aacctgaaag aatgccggag aacttcacca attcatcacc tgcattgagc agattttgca
    13501 ggttctcaat aaccggtaat ccagccccaa cgttggtgtc atagaggaat ttacgccgcg
    13561 atttttccgc cgcataacgc aactgatgat agtaatccat cgacgaggtg ttggcctttt
    13621 tgttcggcgt gacaacgtgg aaaccttcgc gcaggaagtc ggcatattga tccgccactg
    13681 cctggctgga ggtgcagtca acaatgaccg ggttcagcag atgatattct ttcacgaggc
    13741 gaattaagcg cccgagatta aacggctctt tggcttgtgc aagttcttcc tgccagtttt
    13801 ccagattaag gccatgcaca ctagtgagca gagccttcga gttggcaacg ccgcagacac
    13861 gtaagtcgat atgtttattc ttcagccagc tttgctgacg cttcagttgc tccagcagcg
    13921 caccgccaac gccaccgacg ccaatcacaa acacttcgat aacctgatcg gtattgaaca
    13981 gcatctgatg agtaacgcgc acgccagtgg tcgcatcatc gttatttacc acgacagaga
    14041 ttgagcgttc agaagatccc tgagcaatgg cgacaatgtt gatattggcg cgggccagcg
    14101 cggcaaagaa tttcgccgag atcccacgca aggtgcgcat accatcacct accaccgaga
    14161 taatggccag ccgttccgtc accgccagcg gctccagtaa gccttctttc agttccaggt
    14221 agaactcttc ctgcattgcc cgttcagctc gcacacagtc gctttgtgga acgcagaaac
    14281 tgatactgta ttcggaagat gattgcgtaa tcagcaccac ggaaatacgg gcgcgtgaca
    14341 ttgcagcaaa gacgcgcgcc gccatgccaa ccatcccttt catccccggg ccggaaacgc
    14401 tgaacattgc catgttattc agattggaaa tgcccttgac cggtaattcg tcttcatcac
    14461 ggctggcacc aatgagcgta cctggtgctt gaggatttcc ggtattttta atcaggcaag
    14521 ggatctggaa ctgggcgatg ggggtaatgg tgcgggggtg aagaacttta gcgccgaagt
    14581 aggaaagctc catcgcttcc tggtaggaca tcgacttcaa caacctcgca tcgggcacct
    14641 gacgcgggtc gcaggtatag accccgtcaa cgtccgtcca aatctcgcaa caatcggcgc
    14701 gtaaacaggc agccagcacc gccgcggagt agtcggaacc gttacgtcca agcaccacca
    14761 gttcgccttt ttcattaccg gcggtgaaac ctgccatcaa caccatgtga tcagccggaa
    14821 tgcggcttgc cgcaatacgg cgggtggact cagcaatatc gacggtagat tcgaggtaat
    14881 gccccactgc cagcagtttt tcgaccggat cgataacggt aacgttgtga ccacgcgctt
    14941 ctaacacgcc ggccataatg gcgatcgaca ttttctcgcc acggcaaatc agcgcagcgt
    15001 tgatgctatc cgggcactgc cccaacaaac taatgccatg caggacatgt tttatttggg
    15061 caaattcctg atcgacgaaa gttttcaatt gcgccagcgg gaaccccggc tgggcggcgg
    15121 cgagtcccgt caaaagttcg gcaaaaatac gttcggcatc gctgatattg ggtaaagcat
    15181 cctggccgct aatgtttttt tcaatcatcg ccaccaggtg gttggtgatt ttggcggggg
    15241 cagagaggac ggtggccacc tgcccctgcc tggcattgct ttccagaata tcggcaacac
    15301 gcagaaaacg ttctgcattt gccactgatg taccgccgaa cttcaacact cgcatggttg
    15361 ttacctcgtt acctttggtc gaaaaaaaag cccgcactgt caggtgcggg cttttttctg
    15421 tgtttcctgt acgcgtcagc ccgcaccgtt acctgtggta atggtgatgg tggtggtaat
    15481 ggtggtgcta atgcgtttca tggatgttgt gtactctgta atttttatct gtctgtgcgc
    15541 tatgcctata ttggttaaag tatttagtga cctaagtcaa taaaatttta atttactcac
    15601 ggcaggtaac cagttcagaa gctgctatca gacactcttt ttttaatcca cacagagaca
    15661 tattgcccgt tgcagtcaga atgaaaagct gaaaaatact tactaaggcg ttttttattt
    15721 ggtgatattt ttttcaatat catgcagcaa acggtgcaac attgccgtgt ctcgttgctc
    15781 taaaagcccc aggcgttgct gtaaccagtc gaccagtttt atgtcatctg ccactgccag
    15841 agtcgtcagc aatgccatga ctcgttcgcg taaagcttgc agttgatgtt ggtctgccgt
    15901 tgtgtcactt ttcgccggtt gttgtattaa tgttgctaat tgatagcaat agaccatcac
    15961 cgcctgcccc agattgagcg aaggataatc cgccaccatc ggcacaccag taagcacgtc
    16021 agccaacgct aactcttcgt tagtcagccc ggaatcttcg cgaccaaaca ccagcgcggc
    16081 atggctcatc catgaagatt tttcctctaa cagcggcacc agttctactg gcgtggcgta
    16141 gtaatgatat ttcgcccgac tacgcgcagt ggtggcgaca gtgaaatcga catcgtgtaa
    16201 cgattcagcc agtgtcggga aaactttaat attatcaata atatcaccag atccatgtgc
    16261 gacccagcgg gtggctggct ccaggtgtgc ctgactatcg acaatccgca gttcgctaaa
    16321 acccatcgtt ttcattgccc gcgccgctgc cccaatattt tctgctctgg cgggtgcgac
    16381 caggataatc gttatacgca tattgccact cttcttgacc aaataaccgc gaacagggtg
    16441 atcactgtca acttattacg cggtgcgaat ttacaaattc ttaacgtaag ccgcagaaaa
    16501 agccctttac ttagcttaaa a
//
LOCUS       DAZXUK010000070        16449 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZXUK010000070 DAZXUK010000000
VERSION     DAZXUK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16449
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.070"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1238
                     /locus_tag="ACH3L8_001802"
                     /pseudo
     CDS             1..1238
                     /locus_tag="ACH3L8_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
     gene            1388..1819
                     /gene="ndk"
                     /locus_tag="ACH3L8_001803"
     CDS             1388..1819
                     /gene="ndk"
                     /locus_tag="ACH3L8_001803"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFT4084947.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            1969..3123
                     /locus_tag="ACH3L8_001804"
     CDS             1969..3123
                     /locus_tag="ACH3L8_001804"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFT4084948.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            3408..4421
                     /gene="rodZ"
                     /locus_tag="ACH3L8_001805"
     CDS             3408..4421
                     /gene="rodZ"
                     /locus_tag="ACH3L8_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFT4084949.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            4448..5575
                     /gene="ispG"
                     /locus_tag="ACH3L8_001806"
                     /gene_synonym="gcpE"
     CDS             4448..5575
                     /gene="ispG"
                     /locus_tag="ACH3L8_001806"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFT4084950.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            5678..6952
                     /gene="hisS"
                     /locus_tag="ACH3L8_001807"
     CDS             5678..6952
                     /gene="hisS"
                     /locus_tag="ACH3L8_001807"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFT4084951.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            6970..7590
                     /gene="yfgM"
                     /locus_tag="ACH3L8_001808"
     CDS             6970..7590
                     /gene="yfgM"
                     /locus_tag="ACH3L8_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFT4084952.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            7601..8779
                     /gene="bamB"
                     /locus_tag="ACH3L8_001809"
     CDS             7601..8779
                     /gene="bamB"
                     /locus_tag="ACH3L8_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFT4084953.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            8897..10369
                     /gene="der"
                     /locus_tag="ACH3L8_001810"
     CDS             8897..10369
                     /gene="der"
                     /locus_tag="ACH3L8_001810"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFT4084954.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            10439..10654
                     /locus_tag="ACH3L8_001811"
     CDS             10439..10654
                     /locus_tag="ACH3L8_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFT4084955.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(10651..12021)
                     /gene="xseA"
                     /locus_tag="ACH3L8_001812"
     CDS             complement(10651..12021)
                     /gene="xseA"
                     /locus_tag="ACH3L8_001812"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFT4084956.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            12183..13649
                     /gene="guaB"
                     /locus_tag="ACH3L8_001813"
     CDS             12183..13649
                     /gene="guaB"
                     /locus_tag="ACH3L8_001813"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFT4084957.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            13718..15295
                     /gene="guaA"
                     /locus_tag="ACH3L8_001814"
     CDS             13718..15295
                     /gene="guaA"
                     /locus_tag="ACH3L8_001814"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFT4084958.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(15390..15929)
                     /locus_tag="ACH3L8_001815"
     CDS             complement(15390..15929)
                     /locus_tag="ACH3L8_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFT4084959.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(15945..>16449)
                     /locus_tag="ACH3L8_001816"
     CDS             complement(15945..>16449)
                     /locus_tag="ACH3L8_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFT4084960.1"
                     /translation="KCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETKTVN
                     IISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGGAVG
                     AAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNETRIQPN
                     TKCPEKS"
BASE COUNT         3815 a         4089 c         4561 g         3984 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgtc gccccactgg catggaaaac gggcaccagc tatggctatc
      481 gtgacgcctg ggcgattggg gttaacgctc gctatgtcat tgggatctgg actggcagac
      541 ctgacggcac gcccgttgtt ggtcagtttg gctttgccag tgccgtacca ttgttgaatc
      601 aggtcaataa tatcttactg tcgcgcagtg cgaatctgcc ggaagacccg cgcccgaact
      661 cagtcactcg tggcgttatc tgctggccgg gcggacagtc tttgccggaa ggtgacggta
      721 actgtcgccg ccgcctggca acctggctgc tggatggcag ccagccgcca acgctgttac
      781 tgtcagagca ggaagggatt aatggcattc gcttccccat ctggctggat gacaaagggc
      841 agcgcgtcgc cgctgactgc ccgcaggcga agcaagaaat ggtaaatgtc tggccgctac
      901 cgctggaacc ctggctgccc gcatcagaac gtcgcgctgt gcgcttacca ccggcctcga
      961 caatttgccc gccatacggt cacgatgccc agctccctct gcagctgaca ggcgttcgcg
     1021 atggcgcgat tattaaacgt ttaccgggcg cagcggaagc gactttgccg ttgcaatcaa
     1081 gtggaggggc aggtgaacgc tggtggtttc tgaatggcga accgttaact gaacgcgggc
     1141 gcaacgtgac cctgcatttg acggataaag gtgattatca attgctggtg atggatgatg
     1201 tggggcaaat cgcgacagtg aaatttgtca tgcaatagtc tgttttgtcc ctgattgttg
     1261 ctaaaactca ttttatttta aaaaaaatgt taccttcatc aatagtcaac ggccctgttg
     1321 ctcattataa tccgcgccat ctcgtacgct ggtacagaca acaacagaac aatttacaga
     1381 ggtaaaaatg gctattgaac gtactttttc catcatcaaa ccgaacgcgg tagcaaaaaa
     1441 cgtcattggt aatatctttg cgcgctttga agctgcaggg ttcaaaattg tcggcaccaa
     1501 aatgctgcat ctgaccgttg aacaggcacg tggcttttat gctgaacacg atggaaaacc
     1561 gttctttgat ggtctggttg aattcatgac ctctggcccg atcgtggttt ccgtgctgga
     1621 aggtgaaaac gccgttcagc gtcaccgcga tctgctgggc gcgaccaatc cggcaaacgc
     1681 actggctggt actctgcgcg ctgattacgc tgacagcctg accgaaaacg gtacccacgg
     1741 ttctgattcc gtcgaatctg ccgctcgcga aatcgcttat ttcttcggcg aaggcgaagt
     1801 gtgcccgcgc acccgttaat aatttcgtaa atgccgcgtg caaacgtggc atccgtgcgc
     1861 cagaatttgt acaatgcagc gcccccggac gagcagccgc tcaccggggc gtttcttttt
     1921 tcaaccctcc aggggccata acgtgtaata acgaggccgg aatagcatat gtctgaacaa
     1981 ttagtcacac ctgaaaacgt caccacgaaa gatggaaaaa tcaacctgct ggatctcaac
     2041 cgtcagcaga tgcgggagtt ttttaaagat ttaggtgaaa aaaccttccg cgccgatcag
     2101 gtgatgaagt ggatgtatca ctattgctgc gacaactttg atgagatgac cgacatcaac
     2161 aaagtgttgc gcggcaaact gaaagaggtg gcggaaatcc gcgcaccgga agtggttgaa
     2221 gaacagcgtt catccgacgg caccattaaa tgggcgatcg ccgttggcga tcagcgcgtc
     2281 gaaacggtgt atatcccgga agacgaccgt gccacgctct gcgtctcttc gcaggtgggg
     2341 tgtgcgctgg agtgtaaatt ctgttccacc gcccagcagg gctttaaccg caacctgcgg
     2401 gtatcggaaa ttattggcca ggtgtggcgt gcggcgaaaa tcgtcggcgc agcaaaagtc
     2461 accggtcagc gcccaatcac taacgtcgtg atgatgggca tgggcgagcc gctgctcaac
     2521 ctgaacaacg tcgttccggc gatggaaatc atgcttgatg atttcggttt tggcctgtct
     2581 aaacgtcgcg tgacgctttc cacttccggt gtagttccgg cgctggataa actgggcgat
     2641 atgatcgacg ttgcactggc aatttccctg cacgcgccga acgacgaaat tcgtgacgaa
     2701 attgtgccga tcaacaaaaa gtacaatatc gaaacgttcc ttgctgcggt gcgccgttat
     2761 ctggagaaat ccaacgccaa tcagggccga gtcactattg aatacgtgat gcttgaccac
     2821 gttaacgacg gcactgaaca cgcgcaccaa ctggcggaac tgctgaaaga tacgccgtgt
     2881 aagatcaacc tgatcccgtg gaacccgttc ccggacgcgc cgtatggacg cagttcgaac
     2941 agccgtatcg accgtttctc aaaagtgctg atgagctacg gtttcaccac tattgtgcgt
     3001 aaaacgcgtg gtgatgatat cgatgctgcc tgtggtcagt tggcgggcga tgttatcgac
     3061 cgtacgaaac gtactctgcg taaacggatg cagggtgaag ccatcgatat taaagcggtc
     3121 tgatagccat tacgccacgg ttactttatg taccgtggcg taatctttta tgaatgaagt
     3181 attaagttgc aattgtctgg tcaatattaa cggtgcgttt ttgctgactt taaggcagta
     3241 tgtaacgatg caacagtaag ttagcctcag tgaatgtggg ctttgtcacg agcacacaga
     3301 cggtcttata ctgtatgata acggttaact taacggatgt ttcgcggtgt gggtgagcat
     3361 gatggttcac cggcatctca attctcattt aaacgtacct gcagcgaatg aatactgaag
     3421 ccacgcacga ccaaaatgaa gcacttacta ccggcgctcg cctgcgtaat gctcgcgaac
     3481 aactaggact tagtcagcag gccgttgccg agcgactttg cctgaaggtt tccacggtac
     3541 gcgacattga agaagataag gcacccgccg atcttgcttc aacattcctg cgcggatata
     3601 tccgctctta tgcgcgtctg gtacatattc cagaagaaga actgctgcca gggctggaaa
     3661 agcaggctcc acttcgggct gcaaaagttg cgccaatgca gagtttttcc ctcggtaaac
     3721 gccgcaaaaa acgcgacggc tggctaatga ccttcacctg gctggtgttg tttgtggtta
     3781 tcggcctgag cggtgcctgg tggtggcaag accacaaagc tcagcaggaa gagatcacca
     3841 ctatggccga tcaatcttcg gcggaactga gcagtaatag cgagcagggg cagagtgttc
     3901 cgttaaatac gtcgacaact acagacccgg ctacaacgtc cacgccgcca gcgtctgtgg
     3961 atactaccgc aaccaacacg caaacacctg ccgtaactgc gccagcacca gctgttgatc
     4021 cgcaacagaa tgcggttgtt tcgccttcgc aggcaaatgt tgataccgcc gcgaccccgg
     4081 taccaacggc agcaacaacg ccagatggtg ctgcgccgtt gccaaccgat caggctggcg
     4141 tgaccacgcc ggcggctgat ccgaatgcgc tggtgatgaa ctttactgcc gattgctggc
     4201 tggaggtcac tgatgctacc ggtaaaaaat tgtttagcgg gatgcagcgt aaagacggta
     4261 atttgaattt aaccggccag gcaccgtata aactgaaaat tggtgcgcca gccgcagtac
     4321 agatccagta tcaagggaaa cctgtcgatc tgagtcgttt tatcagaact aaccaggttg
     4381 cgcgtctgac cctcaatgcc gaacaatcac cggcgcagta acagacgggt aacgcgggag
     4441 atttttcatg cataaccagg ctccaattca acgtagaaaa tcaacacgta tttacgttgg
     4501 gaatgtgccg attggcgatg gtgctcccat cgccgtacag tccatgacca atacgcgtac
     4561 gacagatgtc gaagcaacgg tcaatcaaat caaggcgctg gaacgcgttg gcgctgatat
     4621 cgtccgtgtc tccgtaccga cgatggacgc ggcagaagcg ttcaaactca tcaaacagcg
     4681 ggttaacgtg ccgctggtgg ctgacatcca cttcgactat cgcattgcgc tgaaagtagc
     4741 ggaatacggc gtcgattgtc tgcgtattaa ccctggtaat atcggtaatg aagagcgtat
     4801 tcgcatggtg gttgactgtg cgcgcgataa aaacattccg atccgtattg gcgttaacgc
     4861 cggatcgctg gaaaaagatc tgcaagaaaa gtatggcgaa ccgacgccgc aggcgttgct
     4921 ggaatccgcc atgcgtcatg ttgatcatct cgatcgcctg aacttcgatc agttcaaagt
     4981 cagcgtgaaa gcgtctgacg tcttcctcgc tgttgagtct tatcgtttgc tggcaaaaca
     5041 gatcgatcag ccgctgcatc tggggatcac cgaagccggt ggtgcacgca gcggggcagt
     5101 aaaatccgcc attggtttag gtctgctgct gtcggaaggc atcggcgaca cgctgcgtgt
     5161 atcgctggcg gccgatccgg tcgaagagat caaagtcggt ttcgatattt tgaaatcgct
     5221 gcgtatccgt tcgcgaggga ttaacttcat cgcctgcccg acctgttcgc gtcaggaatt
     5281 tgatgttatc ggtacggtta acgcgctgga gcaacgcctg gaagatatca tcactccgat
     5341 ggacgtttcg attatcggct gcgtggtgaa tggcccaggt gaggcgctgg tttctacact
     5401 cggcgtcacc ggcggcaaca agaaaagcgg cctctatgaa gatggcgtgc gcaaagaccg
     5461 tctggacaac aacgatatga tcgaccagct ggaagcacgc attcgtgcga aagccagtca
     5521 gctggacgaa gcgcgtcgaa ttgacgttca gcaggttgga aaaataataa cgtgatggga
     5581 agcgcctcgc ttcccgtgta tgattgaacc cgcatggctc ccgaaacatt gagggaagcg
     5641 ttgagggttc atttttatat tcagaaagag aataaacgtg gcaaaaaaca ttcaagccat
     5701 tcgcggcatg aacgattacc tgcctggcga aacggccatc tggcagcgca ttgaaggcac
     5761 actgaaaaac gtgctcggca gctacggtta cagtgaaatc cgcttgccga ttgtagagca
     5821 gaccccgcta ttcaaacgtg cgattggtga agtcaccgac gtggttgaaa aagagatgta
     5881 cacctttgag gatcgcaatg gcgacagcct gactctgcgc cctgaaggga cggcgggctg
     5941 tgtacgcgcc ggcatcgagc atggtcttct gtacaatcag gaacagcgtc tgtggtatat
     6001 cgggccgatg ttccgtcacg agcgtccgca gaaagggcgt tatcgtcagt tccatcagtt
     6061 gggctgcgaa gttttcggtc tgcaaggtcc ggatatcgac gctgaactga ttatgctcac
     6121 tgcccgctgg tggcgtgcgc tgggtatctt cgaacacgta actcttgagc tgaattctat
     6181 cggttcgctg gaagcacgcg ccaattaccg cgatgcgctg gtggcattcc ttgagcagca
     6241 taaagaaaag ctggacgaag actgcaaacg ccgcatgtac actaacccgc tgcgcgtgct
     6301 ggattcaaaa aatccggaag tgcaggcgct tctcaacgac gctccggcat taggtgatta
     6361 tctggacgag gaatctcgtg agcattttgc cggtctgtgc aaactgctgg agagcgcggg
     6421 gatcgcttac accgtaaacc agcgtctggt gcgtggtctg gattactata accgtaccgt
     6481 tttcgagtgg gtgactaaca gtctcggctc ccagggcacc gtgtgtgcag gcggtcgtta
     6541 tgacggtctt gtggaacaac tgggcggtcg tgcaacaccg gctgtcggtt ttgcgatggg
     6601 cctcgaacgt cttgtattgt tagtacaggc cgttaatccg gaatttaaag ccgatcctgt
     6661 tgtcgatata tacctggtgg cttcaggtgc tgatacacaa tctgcggcta tggcattagc
     6721 tgagcgtctg cgtgatgaat tactgggcgt gaaattgatg accaaccacg gcggcggcaa
     6781 ctttaagaaa cagtttgccc gtgctgataa atggggtgcc cgcgttgctg tggtgctggg
     6841 tgagtctgaa gtggctaacg gcacagcagt agtgaaggat ttgcgctctg gtgagcaaac
     6901 ggcagttgcg caggatagcg tagccgcgca tttgcgcacg ttactgggtt aaggaaggag
     6961 aaggacagcg tggaaattta cgagaacgaa aacgaccagg tagaagcggt taaacgcttt
     7021 tttgctgaaa atggcaaagc actggctgtt ggggggattt tgggcgttgg cgcactgact
     7081 ggctggcgct actggaacag ccatcaggtt gattctgcac gctccgcttc tcttgcctat
     7141 caaaatgcgg ttaccgcagt gagcgaaggc aaaccggata gcatcccggc ggcggaaaaa
     7201 tttgctgctg aaaataaaaa tacttatggt gcgctggctt ctttggaact tgcgcagcaa
     7261 tttgttgaca aaaatgaact agagaaagct gccgcccagt tacaacaggg gctggcagac
     7321 acgagcgatg aaaatctcaa agccgtgata aatctgcgtc ttgctcgcgt tcaggtacag
     7381 ctcaagcagg ctgatgccgc gctgaaaacc cttgatacca tcaaaggtga agggtgggct
     7441 gccattgttg ccgacttgcg tggtgaagca ttgctgagca aaggtgataa gcaaggtgcg
     7501 cgtagtgcat gggaagcagg cgtgaaaagc gatgttactc cggcactgag cgaaatgatg
     7561 cagatgaaaa ttaataattt gtccatctga gagggacccg atgcaattgc gtaaattact
     7621 gctgccagga ctgctttccg ttaccctttt aagcggctgt tcgctgttta acagcgaaga
     7681 agatgtggta aagatgtccc cattgccaac cgttgaaaac cagtttacgc cgaccacggc
     7741 gtggagcact tccgttggta gcggcattgg caacttctat tccaatcttc atccggcact
     7801 ggcggacaac gttgtctatg cagcggaccg cgctggtttg gtaaaagcgc tgaatgcgga
     7861 tgatggcaaa gaaatctggt ctgtcagcct ggccgagaaa gatggctggt tctctaaaga
     7921 gcctgcatta ctttctggcg gtgtgaccgt gtctggtggg catgtctaca ttggcagcga
     7981 aaaggcgcag gtttacgcgc tgaataccag cgatggtact gtggcatggc aaactaaagt
     8041 cgcgggtgaa gcactttcgc gcccggtggt cagcgacggt ctggtgttaa tccacaccag
     8101 taacggtcag ttacaagcgc tgaacgaagc tgacggcgct gtcaaatgga cagttaacct
     8161 cgatatgcct tcgctctctt tgcgtggcga gtctgcgccg gcaacggctt ttggtgcggc
     8221 cgtcgtgggt ggcgataatg gtcgcgtcag cgcagtgctg atggaacagg gccagatgat
     8281 ttggcagcag cgtatttccc aggcgaccgg ttctaccgaa attgaccgtc tgagcgatgt
     8341 tgacacgact cccgtcgttg ttaacggcgt tgttttcgcg ctggcctata atggtaacct
     8401 gacggcgctt gatctgcgca gtggtcagat tatgtggaaa cgcgaactgg gttcggtgaa
     8461 tgatttcatc gtcgatggca atcgcatcta tctggtcgat caaaatgacc gggtgatggc
     8521 gttgaccatt gatggcggcg ttacgctgtg ggcacaaagc gatctgctgc atcgcctgct
     8581 gacttctccg gtgctgtata atggcaacct ggtggtcggt gacagtgaag gttatctgca
     8641 ctggattaac gtcgaagatg gtcgtttcgt tgcccagcaa aaagttgata gttccggttt
     8701 ccagactgaa ccggttgccg ctgacggcaa actgctgatc caggcaaaag acggaaccgt
     8761 gtactctatt acacgttaat cgtctctgtc gttcactttg aaaacggctc ctggacaggg
     8821 gccgttttcc tgtttttaac aacgacgcga atatagcgtg cgttgtctga tgatttataa
     8881 aaatgaggct ttaaacatgg tacctgtggt cgcgcttgtc gggcgcccta acgtaggaaa
     8941 atccacgtta tttaaccgtc taactcgcac ccgagatgcg ctggttgcgg atttcccggg
     9001 tctgactcgt gaccgtaagt acggtcgtgc ggaaattgaa ggccgtgagt ttatctgtat
     9061 tgataccggc gggattgatg gcacagaaga cggtgtagaa acccgcatgg cggaacagtc
     9121 gctgctggcg attgaagaag cggacgtcgt actgtttatg gtggatgcgc gcgcgggcct
     9181 gatgccggca gatgaagcga ttgccaaaca tctgcgctcc cgtgaaaaac cgaccttcct
     9241 ggtggtaaac aaaactgacg gtctggatcc cgatcaggca gtggttgatt tctactcgct
     9301 tggtttaggt gaaatctacc cgatcgccgc gtctcacggt cgtggcgtat taagtctgct
     9361 ggagcatgtg ctgctgccgt ggatggaaga tctcgcaccg caagaggaag tcgacgaaga
     9421 cgctgaatac tgggcgcaat ttgaagcgga agagaacggc gaagaagaag aggaagacga
     9481 cttcgacccg caaagtctgc cgatcaaact ggcgattgtg ggtcgtccga acgtaggtaa
     9541 gtctacactc actaaccgta ttcttggaga agagcgcgtt gttgtttacg acatgcctgg
     9601 cacgacgcgt gacagcatct acatcccaat ggaacgcgat ggacgtgagt atgtgctcat
     9661 tgacaccgct ggcgtacgta aacgcggcaa aatcaccgat gctgtagaga aattctccgt
     9721 aatcaaaacg ttgcaggcca ttgaagacgc caacgtggtg atgttagtga ttgatgcgcg
     9781 cgaaggtatt tccgatcagg atctctcgct gctgggcttt attctcaata gtgggcgctc
     9841 acttgtcatt gtggtgaata agtgggatgg cctgagccag gaagtgaaag agcaggtgaa
     9901 agaaacgctg gacttccgtc tgggctttat cgattttgct cgtgtgcact ttatctctgc
     9961 cttgcacggc agtggtgttg gtaacttgtt tgaatcagta cgtgaagcgt atgacagctc
    10021 cacccgtcgt gtggggacct ctatgctgac gcgcatcatg acgatggctg ttgaagatca
    10081 ccaaccgccg ctggtacgcg gtcgtcgtgt gaagctgaaa tatgcccacg ctggtggtta
    10141 taacccgccg attgtggtga ttcacggtaa tcaggtgaaa gacctgcctg attcctacaa
    10201 gcgctacttg atgaactact tccgcaaatc gctggacgta atgggatcgc cgattcgtat
    10261 tcagttcaaa gaaggggaaa acccgtatgc gaacaagcgt aacaccctga cgccaaccca
    10321 gatgcgtaaa cgtaagcgtc tgatgaagca catcaagaaa aataaataat tatccgtaag
    10381 tattttatct gccattccct ctatgaatgt agagggaatg gctaccggag gaattcatat
    10441 ggaactgcat tgtccaaaat gtcagcatgt gcttgatcag gataacggcc atgcgcgttg
    10501 cccatcctgc ggggaattta ttgaaatgaa agctctttgt ccagactgcc atcaaccgtt
    10561 gcaggtgtta aaagcctgcg gtgcagtaga ttacttctgt cagcacgggc atggcttgat
    10621 atcgaaaaaa cgcgttgaat tcgtgctggc ttaatgaacc tttttacgcg atttttttac
    10681 cggctggatg ttttttactt cactttctat ccagccgtct tccagacgtg tggttagcat
    10741 ttcacccgtt ttaacttgct tcactttttt cagtacattg ccgtcagtag cagtagtaac
    10801 gctgtatcca cgcgccagcg ttgacagtgg gcttacggct tcgaggtgcg ttactgcatt
    10861 accgaaacgt tcacgcgtgg cgctaagctg tgcgcgcagg atttctgcta aacgatattc
    10921 cagttgctga atgcgcgttt gcgtgcgatg aatcttcggc tgtggatttt gctgattcag
    10981 ccgctgtgtt aatcgctgct gctgttgccc ggcacgctta agctgatttt ccagcgcaaa
    11041 gctcatccgt ttttgcaggc gttcaagcat ggtttgctgg cgtgccagcc ggagctgcgg
    11101 atgctgctgc tgcaagcgat gatgaatctg cgtaaagcga cgtgtacggt tggcgagata
    11161 ataatccatc gccatctcca gccgttgacg ggccgattgc acctggcgca gtaactcttg
    11221 ctgattacgg ctcactactt cagcggcggc agacggcgtt ggcgcacgca gatcggcaac
    11281 aaaatcggca atggtcacat ccgtctcatg cccgacggcg ctgacgaccg gaatgcggct
    11341 ggcaaaaatc gcccgcgcta cgcgttcgtc gttaaaactc cataaatctt ccagcgaacc
    11401 gccgccgcgc ccaacgatca acacgtcgca ctcattgcgc tgattcgcca gttcaatggc
    11461 gcgaacaatt tgccccggcg cgtcatcgcc ctgaacggag gtggggtaga tgatcaccgg
    11521 tagagaagga tcgcgacgtt ttaacacatg caaaatatca tgtagcgcag caccggtttt
    11581 tgaggtgatc acaccaacgc aatgcgcagg ggagggaagt ggttttttgt attgctgatc
    11641 gaacaaacat tcagcctgca acttcgcttt gagctgttcg tacttctgtt gcagcagccc
    11701 ttcaccggcc ggctgcatac tctcaacgat tatctggtag tcgccgcgcg gctcgtagag
    11761 cgtaatattg gcgcgaacta aaacttgttg cccatgctgc ggacggaagg tgacccggcg
    11821 gttgctgttg cggaacatcg cgcagcgtac ctgggcggtg tcgtctttga gtgtaaagta
    11881 ccagtgaccg gaagccggtt gtgtgaaatt agagatttcg ccgctgatcc aaacctgtcc
    11941 catctcatgc tcaagcagca gacgaaccgt ttgattcagg cgactaacgg taaaaattgc
    12001 aggggattga gaaggtaaca tgtgagcgag atcaaattct aaatcagcag gttattcagt
    12061 ctatagtaac ccgcccttcg gggatagcaa gcattttttg caaaaagggg tagatgcaat
    12121 cggttacgct ctgtataatg ccgcggcaat atttattaac cactctggtc gagatattgc
    12181 ccatgctacg tatcgctaaa gaagctctga cgtttgacga cgttctcctc gttcctgctc
    12241 attctaccgt tctgccgaat actgctgacc tcagcaccca gctgacgaaa actattcgtc
    12301 tgaatatccc tatgctttcc gcagcaatgg ataccgtaac ggaagcgcgc ctggctattg
    12361 ctctggctca ggaaggcggt atcggcttta tccacaaaaa catgtccatt gaacgccagg
    12421 cagaagaagt tcgccgtgtg aaaaaacacg aatctggtgt ggtgactgat ccgcagactg
    12481 ttctgccaac cacgacgctg cgcgaagtga aagaactgac cgagcgtaac ggttttgcgg
    12541 gctatccggt cgttaccgaa gaaaacgaac tggtgggtat tatcaccggt cgtgacgtgc
    12601 gttttgttac cgacctgaac cagccggtta gcgtttacat gacgccgaaa gagcgtctgg
    12661 tcaccgtgcg tgaaggtgaa gcccgtgaag tggtgctggc aaaaatgcac gaaaaacgcg
    12721 ttgaaaaagc gctggtggtt gatgacgaat tccacctgat cggcatgatc accgtgaaag
    12781 acttccagaa agcggaacgt aaaccgaacg cttgtaaaga cgagcaaggc cgtctgcgtg
    12841 ttggtgctgc agttggcgca ggtgcgggta acgaagagcg tgttgacgcg ctggttgccg
    12901 ctggcgttga cgttctgctg atcgactcct cccacggtca ctcagaaggc gttttgcagc
    12961 gtatccgtga aacccgtgct aaatatccgg atctgcaaat catcggcggc aacgtggcaa
    13021 cagctgcagg tgcacgcgct ttggcagaag ctggttgcag cgcggttaaa gtcggtatcg
    13081 gccctggttc tatctgtacg actcgtatcg ttactggcgt aggtgttccg cagatcactg
    13141 ccgttgctga cgcagttgaa gccctggaag gcaccggtat tccggttatc gcagatggtg
    13201 gtattcgctt ctccggcgac atcgccaaag ctatcgccgc tggcgcaagc gcggtgatgg
    13261 taggttccat gctggcgggt actgaagaat ctccgggtga aatcgaactc taccagggcc
    13321 gttcttataa atcttaccgt ggtatgggtt ccctgggcgc gatgtccaaa ggttcctctg
    13381 accgttattt ccagagcgat aacgctgccg acaaactggt gccggaaggt atcgaaggtc
    13441 gcgtagctta taaaggtcgc ctgaaagaga tcattcacca gcagatgggc ggcctgcgct
    13501 cctgtatggg tctgaccggc tgtggtacta tcgacgaact gcgtactaaa gcggagtttg
    13561 tacgtatcag cggtgcgggc attcaggaaa gccacgttca cgacgtgacc attactaaag
    13621 agtccccgaa ctaccgtctg ggctcctgat tctcttcgcc cgatttcatg tcgggcgatt
    13681 tatattatct gtttcacttg cctcggaata agcgtcaatg acggaaaaca ttcataagca
    13741 tcgcatcctc attctggact tcggttctca gtacactcaa ctggttgcgc gccgcgtgcg
    13801 tgagctgggt gtttactgcg aactgtgggc gtgggatgtg acagaagcac aaattcgtga
    13861 cttcaatcca agcggaatta ttctttccgg cggcccggaa agtactactg aagaaaacag
    13921 tccgcgtgcg ccgcagtatg tctttgaagc aggcgtaccg atattcggcg tttgctatgg
    13981 catgcagacc atggcaatgc agttgggcgg tcacgttgaa gcctctaacg aacgtgaatt
    14041 tggctacgcg caggtagaag tcgtaaacga cagcgcactg gttcgcggta tcgaagatgc
    14101 gctgaccgca gacggtaaac cgctgctcga tgtctggatg agccacggcg ataaagttac
    14161 cgctatcccg tccgacttcg tcaccgtagc cagcaccgaa agctgcccgt ttgccattat
    14221 ggctaacgaa gaaaaacgct tctatggcgt acagttccac ccggaagtga ctcacacccg
    14281 ccagggtatg cgcatgctgg agcgttttgt gcgtgatatc tgccagtgtg aagccctgtg
    14341 gacgccagcg aaaattatcg acgatgctgt agctcgcatc cgcgagcagg taggcgacga
    14401 taaagtcatc ctcggcctct ctggtggtgt ggattcctcc gtaaccgcaa tgctgctgca
    14461 ccgtgctatc ggtaaaaacc tgacttgcgt attcgtcgac aacggcctgc tgcgtctcaa
    14521 cgaagcagag caggttctgg atatgtttgg cgatcacttt ggtcttaaca ttgttcacgt
    14581 accggcagaa gatcgcttcc tgtcagcgct ggctggcgaa aacgatccgg aagcaaaacg
    14641 taaaatcatc gggcgcgttt tcgttgaagt attcgatgaa gaagcgctga aactggaaga
    14701 cgtgaagtgg ctggcgcagg gcaccatcta ccctgacgtt atcgaatctg cggcttctgc
    14761 aaccggtaaa gcacacgtca tcaaatctca ccacaacgtg ggcggcctgc cgaaagagat
    14821 gaagatgggc ctggttgaac cgctgaaaga gctgttcaaa gacgaagtgc gtaagattgg
    14881 tctggagctg ggcctgccgt acgacatgct gtaccgtcat ccgttcccgg gaccaggcct
    14941 tggcgttcgt gttctgggtg aagtgaagaa agagtactgt gacctgctgc gccgtgctga
    15001 cgccatcttc attgaagaac tgcgtaaagc ggacctgtac gacaaagtca gccaggcgtt
    15061 caccgtcttc ctgccagtac gttccgttgg cgtaatgggc gatggtcgta agtatgactg
    15121 ggttgtctct ctgcgtgctg tcgaaaccat cgactttatg accgcacact gggcgcatct
    15181 gccgtacgat ttcctcggtc gcgtttccaa ccgcattatc aatgaagtga acggtatttc
    15241 ccgcgtggtg tatgacatca gcggcaagcc gccagctacc attgagtggg aatgattttg
    15301 atccctgcac tatgaatgaa caaaaccctc cgttactaca gagggttttt tatcttcaag
    15361 aatcataggc ttgaagttac taacatcgat taattaaacc agctgtccga tttgttctct
    15421 tctgctttgc ccacgctttt catcagatcg cgaccgcctt cagtcatatt tctgtttgcg
    15481 tcagcttcag attgcaccac atcggtttgc gcagctttgt gcttcagttc ctgatcgata
    15541 aattcgtttt cgcgcttaac gcgggcttct tctttcgcca gcgccagttt ttgtttctga
    15601 atctctaagc tgcgtagctc atcttcataa ctttgatcgc gttttttgtc cgcagtggct
    15661 tcggcgtcca gtttatcctg acgagctttc ttatttgccg ctgccgttgc cgctcttttg
    15721 ttcgcggcgg cctgggcatt tgcgcgacgt tgcttctctt gctggatttc cctgttgcgc
    15781 tccgcgaccc attcgtcatg ctgcctttgc tcttcatttt taccttgctg ttccgcttct
    15841 gcgacagcag agagttgatc ctgcaatgat gaggctatag ccggatagct taaggaggct
    15901 aagatggcgc aaagaaaaac tttcttcatg actcctcctg attattagct cttttcagga
    15961 catttggtat ttggctgaat acgcgtttcg ttatacgttg tggtaataac aacggctaaa
    16021 cctgtcgtaa actggcactc tttaccctcc tgggtggagg tatacacttt ggtgccttcc
    16081 ttatatgtta aagaaacacc ttccactaag gttttatcat tcaccataga acccgctgcc
    16141 gcgcctacag ctccgccgcc aactgcccct gccgtcgttc cggaattgct gccagaaccg
    16201 acgttgtggc cgataacacc gccagcgact gcgccaataa gcgcgccgaa ggcttgtgcg
    16261 ttccgtttat tttgggagtt gtctacggca acttttgcgg gaagaatgga aataatatta
    16321 acggttttag tttcttgttt ggtattcagt tgatcggttt gataaacatc ggcggcatga
    16381 tcgtcagcat ttgactggca tcctgccaga gtgaatgacg ctaacattgc cacaggcaga
    16441 agacatttt
//
LOCUS       DAZXUK010000071        16369 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZXUK010000071 DAZXUK010000000
VERSION     DAZXUK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16369
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.071"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(48..611)
                     /gene="pqiC"
                     /locus_tag="ACH3L8_001817"
     CDS             complement(48..611)
                     /gene="pqiC"
                     /locus_tag="ACH3L8_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFT4084961.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(608..2248)
                     /gene="pqiB"
                     /locus_tag="ACH3L8_001818"
     CDS             complement(608..2248)
                     /gene="pqiB"
                     /locus_tag="ACH3L8_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFT4084962.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(2253..3506)
                     /gene="pqiA"
                     /locus_tag="ACH3L8_001819"
     CDS             complement(2253..3506)
                     /gene="pqiA"
                     /locus_tag="ACH3L8_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFT4084963.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(3636..5543)
                     /locus_tag="ACH3L8_001820"
     CDS             complement(3636..5543)
                     /locus_tag="ACH3L8_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFT4084964.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(5555..7663)
                     /gene="rlmKL"
                     /locus_tag="ACH3L8_001821"
     CDS             complement(5555..7663)
                     /gene="rlmKL"
                     /locus_tag="ACH3L8_001821"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFT4084965.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            7907..9016
                     /gene="ycbX"
                     /locus_tag="ACH3L8_001822"
     CDS             7907..9016
                     /gene="ycbX"
                     /locus_tag="ACH3L8_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFT4084966.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(9013..9555)
                     /gene="zapC"
                     /locus_tag="ACH3L8_001823"
     CDS             complement(9013..9555)
                     /gene="zapC"
                     /locus_tag="ACH3L8_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFT4084967.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(9729..10739)
                     /gene="pyrD"
                     /locus_tag="ACH3L8_001824"
     CDS             complement(9729..10739)
                     /gene="pyrD"
                     /locus_tag="ACH3L8_001824"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFT4084968.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(10850..11587)
                     /locus_tag="ACH3L8_001825"
     CDS             complement(10850..11587)
                     /locus_tag="ACH3L8_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFT4084969.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            complement(11553..12068)
                     /locus_tag="ACH3L8_001826"
     CDS             complement(11553..12068)
                     /locus_tag="ACH3L8_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4084970.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(12076..12616)
                     /locus_tag="ACH3L8_001827"
                     /pseudo
     CDS             complement(12076..12616)
                     /locus_tag="ACH3L8_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(12628..13697)
                     /locus_tag="ACH3L8_001828"
                     /pseudo
     CDS             complement(12628..13697)
                     /locus_tag="ACH3L8_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(13688..16288)
                     /locus_tag="ACH3L8_001829"
     CDS             complement(13688..16288)
                     /locus_tag="ACH3L8_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFT4084971.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNRGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
BASE COUNT         4057 a         4397 c         3778 g         4137 t
ORIGIN      
        1 gcctcaggca acataccgga gacggttata tttacaaatc tttataatta aggtagacgc
       61 tttatctctt gtgcaataga agcggcttcc tgactccaga caccggccag cactttaacc
      121 atctcatcgt aaccatcctg agtttgcact ccttccagac ggaacggacg tttgatcagt
      181 tgtccctggt ggttcaacag ccactcacca ctgacaatga ccttgccatc atagcgaccg
      241 ttaaactccg ttacggtaac attgagcgtg tcctgggcgc ttcccagagg ctgggaggca
      301 accacccagc cgggcagttg cgtactcagg ttggcaacca gggtgttgcg caactgttga
      361 tccaacgggc tggcccacaa gttgttgttg gcaatcacat acttcacatc actggtttga
      421 taaaccacac cattccccgc cagatagtca ggaacagtga cctgctctac ccataacaga
      481 cgattgccct ggctggcggt actttgtgta ccgctctgca ccacaggtaa ctggtaatag
      541 tttttattaa tttcgccgga gctgcatccg gccagccaca gtgctgcaat cgtcactagc
      601 cactttttca ttgtttcgcc ctcttcggct ctggatcttt tttgtccttc gcttcaaata
      661 ccagcgcgtt actcttctca ttgagcgttt tcagcaccgg ttgcagttct cgcaacacct
      721 gatcaaggcg ctgcatatcc gccaccatct tgttgtaggc tgcggagcca ggctggaagc
      781 cctgcatgct gcgattcaat tcacgcaacg ttgactgcat atccgtcggc aactgctgca
      841 tcgactggct ggcgaggatc ttgttcatgc tatccagcgt cgtttgcagg tttttcattg
      901 tgcgctgact ttcagaaagc gtactggttg cctgttcaat catcggattc aatggcagtt
      961 tgttgatctt atccaacgct tccatcagtc gttgctggat ttgcgccaag ccgccgctaa
     1021 cagtcgggat aatctgataa ccattaaatt cacgaatacc ggttattgca ggcgtatttg
     1081 gatagaaatc gagatcaaca tacagtgcac cagtgaccag gtttccggtt ttcagcgatc
     1141 cgcgtaaacc acgtttcaac aattcgccaa ggtgctcaac aacatccgca ttttcgccaa
     1201 gctgcatttt cagccgctct ggctcgatac gaatcagtac cggaatacgg taatcatcgt
     1261 taaatgtctg acgcatattc ggcgcaaaga atggcacttt gcttacggta cccaggcgaa
     1321 taccccggaa ttctaccgga gctcccggtt gcagaccgcg taccgaatct ttaaagaaca
     1381 tcagataatc aatgtgatcg gtgtacaacg aatcctgaat gctcttctga tcatcataca
     1441 aaacgaaagc tgttttcggt gccactggct gccctaaatc cagaccttcc ggcacatcaa
     1501 agctaacacc gccactaagc agcgttgtca atgagcccat ctccacacgc atccccgctg
     1561 acgtcagatc aaccgcaatg ccactatctt tccagaagcg aacattgctg gtcaccagtc
     1621 ggtcataagg tgcattgatg aacagttgat aactgatatt gcgtttttgc gtatcgaagg
     1681 tgctggtttc aaccgaacct acccgatagc cacggaacag caccggatct cctggcgaga
     1741 gctgcccggc ttttttgcta tcgagaatca cacggatacc tttcgcatca ggcggggcca
     1801 acggtggtga gtccagcaaa tcgtatttat ccattttgct gcctttcgcg cctggctgca
     1861 gttcgatata aactccagac agcagcgttc ccaggccgct aatcccttcg cgaccaatct
     1921 gcggtttcac cacccaaaag acggtgtctt tatgcagcaa tttttccata ccggaattca
     1981 gccgcgcttt gatttcaacg tgcgtcaaat catcagccag tgtggcgctt tcaaccacgc
     2041 cgacgtcaac gctacggctt ttaatggtgg ttttgccacc ttcaattcct tccgcattcg
     2101 cggtgatcag ggtcacttcc ggtccctgat ggctgtaatg ataaaaaaga acccaggccc
     2161 caatgagcgc cgtgacgata ggaaatatcc acacgggaga ccagttcttc actttctgga
     2221 ttttggcttc cccattatta gattccatac tgtcaggact cctcatgctc tgattcaggt
     2281 tgacgatccc acgacaaacg cgggtcaaac gtcatagcag aaaacattgt cattatgacg
     2341 actaaagcaa acattaatgc acccattgcc ggataaatac tcattaaacc tcccatacgc
     2401 accagcgccg agagcaccgc gataacgaaa acgtcaatca tcgaccagcg gcctacaaac
     2461 tcaacaactt cataaatcaa atgcattctt tcactgtcgc gcttgccatg ccctttggca
     2521 tcccaacaca gccacgcgat ggcgatcatc tttaacgttg gcaccataat actggccaga
     2581 aagatcaccg cagcgacggg ataagagcct tcgctccata acagaatgac cccagcgaga
     2641 atcgtcgacg gcatcttcga gcctaataaa tccgtcacca tgatgggcaa aatattagcc
     2701 ggaagataca gcatgatgga cgttacaagc agcgcgagtg tccactgcag gctgtttcta
     2761 cgccgaacgt accctttggt actacaacgc gggcacacgg gttcatcagc aggaaggatt
     2821 gccgtacaac aggagcacga acgcagcccc tgacgtatcc ccgtgacgcc tggttttagc
     2881 ggctggcgca gttctggcat cggggcgatg tcgtcccata accagcgacg atcaacgcac
     2941 tgaaaagcgc gcagttgcag gacacaaaat aaacaccagg ggagaaagct gctgcctacc
     3001 ccaatgctgc cgtaagccat cagtttaacg aaactgacca gcacacccgc gaggaaaatc
     3061 tccgccattc cccaggtttt gagttgaaaa agcacccgtg ccagttgctc ttttaaacgg
     3121 accggtaatt ccgcgcgatt caccagtaac agaatggtta tcagacaaaa cgcgggaacc
     3181 agttgcacaa acaacaggaa aaaggtgccg aggctggcgt agtcctcaga aaaaagcacg
     3241 ccgggaattt ccagtaatgt aatttcactg gtaactcctg caacgttcat attcacaaaa
     3301 ggaaacaagt tggacagcaa cagcatgaac agtgcagcca acgcataggc ggtgggacgc
     3361 tgccgagggg catcccacgc cacggttaac gttgtgccac accggggaca tgccgctttc
     3421 tgaccatgct caaggcgcgg taacgccacc agcatgtcac actgcgagca caggatgtgc
     3481 ttcgcggcat gatgatgttc gcacattggt actccttaat gatgcgctat tttacagatg
     3541 actaacaaag tgttggtttt catgcctgat aaacccagtt catcaggcaa ttttaccgtt
     3601 gtcagctgtc tctgtttaaa tcgactattt tgcgatcagc caccattttt taacgcttca
     3661 agatactccc agcgttcaaa ggcttgctcc agctcctgct ctgcagcagc catatcagca
     3721 agcacttttt gcgtctgctc atgcggctga ctgaagaagg aagcatccgc cacttgcgtc
     3781 tgtagggctt ccagcttcgc ctccagatct tcgagcaatt gcggtagctg ctccagttcg
     3841 cgctgcaatt tatagcttag tttgctactg ctgcgtttta cagtttctgc ttttggcgcg
     3901 gcggcttctt cgcttttttt caccgcaggc tgtttgagtg ccacatattg ttcttgctga
     3961 ccacgggcat catgataacc gccaacataa cgaccaattt taccgccgcc ttcgaagatc
     4021 cagcattctg taacggtgtt atcgacaaac tgacgatcgt ggctaaccag caataccgtg
     4081 ccctgatagc tgtcgatcag ttcttccagc agttccagcg tttcgacatc aagatcgttg
     4141 gtcggttcgt caagaatcaa taagttgctt ggtttgagga acaaacgcgc cagcagcaag
     4201 cggttccgct caccgccaga aaacgcacgt actggcgtca tcgcccgttt cggatggaac
     4261 agaaagtcct gcaaatagcc caatacgtgg cgtggcttgc cattaaccat cacctcttgc
     4321 ttaccttcgg caaggttatc catcaccgtt ttatcgggat ccagttccgc gcggtgctga
     4381 tcgaaataag ccacttccag tttggtgcca acgtgaatac gcccgctgtc cgcttgaagc
     4441 tgaccgagca tcagtttcag cagcgtggtt ttgccgcacc cattcggacc aatcagggca
     4501 attttgtcgc cacgtagaac ctgggcagaa aaatctttca ccagttgctt accgttaacc
     4561 tggtagcaaa cgtcttccat ttcgaaaacg atcttaccgg agcggctggc ctcttccacc
     4621 tgcatctttg cggtacccat cacttcgcga cgttcaccac gttcgcgacg catcgctttc
     4681 agggcgcgta cgcggccttc attacgggta cggcgtgctt tgatcccctg gcgaatccac
     4741 acttcttcct gcgccagttt gagatcgaac tcggcatttt gtagttcttc cacgcgcagg
     4801 gcttcttctt tttcctgcag gtactggtcg taattccctg gataggtcac cagcttgccg
     4861 cgatcgagat caacaatgcg cgtcgccata ttgcggataa acgaacggtc gtgggagatg
     4921 aaaataatcg tcccattgaa ggttttaagg aacccctcca gccagtcgat ggtttcgata
     4981 tccagatggt tagtcggttc gtccagcagc aatacgcgcg gattgctcac cagcgcgcgt
     5041 cctaatgccg ctttacgcaa ccagccgccg gaaagcgacg acagcgcaac gttaggatct
     5101 aaccccagtt gcgccagcac ttcgttgatg cggttttcca gctgccacag gttgtggtga
     5161 tccagctgtt cctgaacctt cgccagttcg ttgagatttt tctcgctcgg gtcgttcatc
     5221 accaggcgcg aaatatcgtg atagcgtttc agatattccg cttgttcttc aatgccttcg
     5281 gcaacgaaat cataaacgct accctcaacg ttacgcggcg gatcctgttg cagacgagct
     5341 acaatcaaat cttgctcata aataatgcga ccgtcatcca gcccttgttc acggttgagg
     5401 attttcatta acgtcgattt tcctgcgccg ttgcggccca ccagacaaac acgttcgtta
     5461 tcttcgatat gcagttctgc gttatcgaga agcggcgcgt cgctgaacga cagccatgcg
     5521 ccatgcatac tgattaatga cattactatt cctttcaggc tgcggtaatc agccagcagt
     5581 tgtggatctg gcggttacgg gcgaaatcct gggagagcgt tttttgcgta atttcttgtg
     5641 ctttcagtcc cagttttgcc aggccgtcga gatccatacg gaagccacgt ttgttgttcg
     5701 agaacatgat cgtcccacct gcacgcagca gacgtttcaa atctttcatc agcgccagat
     5761 gatcgcgctg aacatcaaac gcatcttcca ttcgttttga gttagagaag gttggcggat
     5821 cgataaagat cagatcgaac tgttcatttg cctcacgcag ccacgccagg caatcggcct
     5881 gaatcaggcg atgcgcacgc ctagtcaggc cattcagacg caggttgcgt tctgcccatt
     5941 ccagataagt acgcgacata tccacggtgg tggtgctgcg tgcaccgcct aatcccgcat
     6001 gcacggtagc gctgccggta taagaaaaca ggttgaggaa atctttgcct ttgctcatct
     6061 gaccgagcat acggcgggcg atacggtgat cgaggaacag accagtatcg agataatccg
     6121 tcaggttcac ccacaagtga gcgttatatt cggtaacttc aagaaactcg cccttctcgc
     6181 ccagtttctg gtactggttt ttgcccttat ggcgttcacg ggttttcagc accagtttgt
     6241 ttggcgcaat ccccagtacc gaaatggttg cagcgataat atcgaacaga cgctgacgcg
     6301 ctttgtgcgc atcaatagtt tttggcggcg catactcctg caccaccacc cagtcggcat
     6361 aacggtcaac ggcaacgtta tattccggca gatcggcgtc atacaggcgg taacattcaa
     6421 tcccttcctg gcgtgcccac ttctcgaatt ttttgaggtt cttacgcagg cggttggcgt
     6481 aatcttccgc taccattgcc ggtttactgt ctggtgtgct ttcggcaacg tggtaatttt
     6541 tctgtacgca gtccagcggg ccgtttttcg ccttgtactg tttgtctgca cgcagctgca
     6601 ggcagcttag cagatccggc gaggcactaa acaaagagag attccagcca ccgaactggt
     6661 ttttcatgat tcggcccagc agactatgca gcgcaatcag cgccggttcg ctgtccagac
     6721 gttcaccgta tggcgggttg ctcaacactg taccgtacgg ccctttcggc agcggattgg
     6781 tcagttgcgc gacatctttc acctcaaagg tgatcagttc accaatcccc gcaagacggg
     6841 cgttagtgcg cgcacgttga atcacccgtg cgtcgctgtc cgaaccgtaa aaatgagagc
     6901 tatactcagc taggccttta cgggcgcgag tttgcgcttc cgctttcact tcctgccaga
     6961 tagcttcatc atgctgcgcc cagccgctaa agccccaacg cccacggtgc aagcctggtg
     7021 cgcgatcggt cgccagcatc gccgcttcaa tcagcaacgt accggaacca cacatcggat
     7081 cgagcagcgg tgttcctgac tgccagccgg atcgcatcac aatagcggct gccagggttt
     7141 ctttgatcgg cgcaatacca gcacgatcgc gatagccacg cagatgtaaa ccatcaccac
     7201 tcagatcaag agcgatactg gcggtttctt tatgcagcca gacgttaacg cggatatccg
     7261 gcgcatcgcg atcaacattt ggacgcggca gatttttccg tgtgaaagca tcgacgatcg
     7321 cgtctttcac tttcatcgcg ccgtactgac tgttgcggat ggtgtcattc aaaccactga
     7381 agtggacagc gaaggtcgcg ccaggattaa acatctctgt ccagttgatc gcctgaacac
     7441 cgagatagag gtctaaatcg ctgtaaacct tacactcgcc cagcggcaac ataatacgcg
     7501 aggccaggcg gctccacatc aggctctggt aaacaagccg tgtgtcgccc ttgaaatgga
     7561 ccccaccctg aaccacctgg cattcaacgg cccccagatt ttccagttca gtttttaaca
     7621 gctcttccag cccacgggcc gtactggcaa acagagaatt catatcgtca cttttactct
     7681 aagagcctac cgcagtaggc gtaattgttg cagtcagtct ggacatggag agcgcggaga
     7741 aaccggagcg tacacaagta cgtgaggatt tcgagcacaa cccagggcca aactgacaaa
     7801 taaaatagcc taacgggaca ggcgctaaga aaattgttgc gcattatagc taataagcgc
     7861 gtcatgtcat aaagttgagg gcttattttc atttgaggac cgcaccgtgg cgacattaac
     7921 ccggcttttt attcatcctg ttaaatcgat gcgcggcatt ggtcttacac atgctctggc
     7981 tgatgtcagt ggtctggcct tcgatcgcat ctttatgatc acggaacctg acggtacgtt
     8041 tattaccgct cgccagtttc cccagatggt acggtttact ccttcacctg tacatgatgg
     8101 cttgcatctc accgcaccag atggcagtag cgcatatgtt cgttttgctg atttcgccac
     8161 acaagacgca ccaaccgaag tttggggcac acattttacc gcgcgaattg cgccagacgc
     8221 gatcaacaaa tggctaagtg gatttttctc ccgcgaagtg caattacgct gggtggggcc
     8281 acaaatgacc cggcgcgtga aacgccacaa cactgtacct ctgtcatttg ctgatggcta
     8341 tccttacctt cttgctaacg aagcctcgtt acgtgatctc caacaacgtt gtccggccag
     8401 tgtaaaaatg gagcaattcc gccccaatct ggtggtttcc ggcgcgtcag cctgggaaga
     8461 agatagctgg aaagtgattc gcattggtga tgtggtgttt gatgtggtta aaccttgtag
     8521 ccgctgtatt ttcaccaccg tcagcccaga aaaagggcaa aaacatccgg caggcgaacc
     8581 attaaaaaca ttgcaatctt tccgcactgc ccaggataac ggcgatgtcg attttggtca
     8641 gaatttaatt gcccgtaata gcggcgtgat tcgcgttggc gatgaggtgg aaattctggc
     8701 aacggctccg gcaaaaattt acggcgcagc tgccgctgat gataccgcca acatcacgca
     8761 acaaccggac gcaaatgtag atattgactg gcagggacag gcatttcgtg gaaataacca
     8821 acaagtgttg ctggagcaat tagaaaatca gggaattcgt atcccttatt cttgccgcgc
     8881 gggcatttgt ggaagttgcc gtgttcagct tttagaaggc gaagtcacgc cgctgaaaaa
     8941 atcagcaatg ggcgatgatg gcaccattct ttgctgtagc tgtgtaccga agactgccct
     9001 taagctgacg cgttagactg cctgttcgag gctgaagctg tcaacattca cctgcggttt
     9061 cagcctgtcg ttcatgattt taatggcgtc gcccaactgc atcgcgcgac ccgcaataac
     9121 aacgcagggt tgtgccagca ggcatagcgc ggcattttcg ccagattcaa cgaccagcaa
     9181 attgacctgc tcgtgagtat cgctcaacca aacgcaggcc gcatcgcccg gcatgggcac
     9241 ccacatttca ccatgggaga caaaatgcca gcttttcggc atttgcggtt tgagataacg
     9301 aattgctacc agtgcattca gcaccagttc ggctttttgg tctttagata aattaaaatc
     9361 gcggcacttt tcttcaaaag agaaatagag cgcggcatca tcaacacaaa agcctgcggg
     9421 agaaaaggca tctggagtca acattttgcg cgcaaaacgt gagcgaaata gcataccatt
     9481 ggctaaatcg agcatcattc gatcgtgctc ttcatcgtaa taccaacgcc agttatcgtc
     9541 tggtttaatt cgcatctgcc tctcccctac cctataaaac cgcagcaaca caatccattg
     9601 tcgcttcgct taataacctc aacaataaaa gtacaaaatg tctaaataag caacaacgat
     9661 gaatataaaa caaccaggtc tgaaaataaa gccctgatta tcttataaag aggaattcga
     9721 agaaataatt agatatgggt aacgatttct ttaatcagcg gcggaccttt aaaaataaaa
     9781 ccagaataaa tttgcaccag tgaggcaccc gcagcaatct tttcacgcgc agcgataacc
     9841 gagtcgatgc cgccaacacc gatgatcggt aagcgaccgt ttaattctag tgacaagcgg
     9901 cgaataattt ctgtgctttt taactgaagc ggacgaccac ttaagccacc ggtttgatcg
     9961 caatttttca ttccctgaac cagagaacga tcgagagtgg tattggttgc aataacgcca
    10021 tcaatattat ggcgaactaa actatcggca acctggatca attcgtcttc agaaagatcc
    10081 ggcgcgatct tcactgcgat cggcacatat ttatggtgca tcgcttgcaa atcattttgc
    10141 ttatttttaa tcgcggttaa gagatcatcc agcgcttcac catattgcag cgtgcgtaat
    10201 cctggggtat tcggcgatga aatattgatg gcgatatatc ccgcataggc atagattttt
    10261 tccatacaaa tcagatagtc atctttgccc tgctccactg gcgtatcttt atttttgccg
    10321 atattaatac ccaagacgcc gtcataatgg gcctttttta cgttctctac gaggttatca
    10381 acgccaagat tattaaagcc catacggttg atcaaacctt cggcatctac cagacgaaag
    10441 agacgcggct tgtcattacc aggctgtgga cgtggcgtga cggtaccgat ctcgatcgat
    10501 ccaaatccca tcgcgcctaa cgcatcaatg cactccccgt ctttatcaag accggctgcc
    10561 agaccaagcg gatttttaaa cgtcaggccc atgcagttaa caggtttcgc aggcactttc
    10621 tgccgcacca gtgcttcaaa cggtgttccg gtaatgcggc gtaattgctg aaaagtaaac
    10681 tcatgagcgc gctctggatc gagctggaaa agggctttac gaacgaaggg gtagtacatg
    10741 aactctcctg gattcccggt gtgcaaaccg ggggcgtatt atgtgcgagt tcggaccaaa
    10801 agggaattga cctgcggcaa aaaaaacgca aacgttttca gaacctgttt cagggcagat
    10861 tttgctcctt ctttgcactc aacccgccgt aatcatttat cgtccgccag gcgacatgac
    10921 acggtgctat tacctgacac cagtttgtct gacgctggct tttaggcggc acgactcccg
    10981 cattacttaa cgctttacca tttacgctca cctgaatcag gttaatgtag taaggcgttg
    11041 ggttagtgag ttgtacaccc tgcgaattcc gtgtccagcg taattgttga taagcttcca
    11101 gcgggtcgcc cggcaaacct tcgggtcgcc agaacagttt aaataccgag cgcatcgcca
    11161 cttttacgct ctgattttca actttgccgg atggcacgct ggcaatgctt aactcaaata
    11221 gcgtttcgcg atccacaggc aagatgtcgc tttccgttct cagcaagcgc aacgtgcctg
    11281 tagtaccggg cttcaggaga ataagtggcg gagcggccag taatagtgct ggcactgtcg
    11341 acgcttcccc cccggcccaa cgcgttgggc gattgatttt gctgttaata agccaagatt
    11401 cctgactggt attagtcagt aaaatagata tcgattctct gtctgccgga aagataaatc
    11461 gcgttccgcc gaccaccact cctgcctgca attcacccgc caccagcagc gataaaacgg
    11521 cgaaaatcaa gagcgctaat ctgttccacg tattagtcat aatgaagtac aaatgtcgcg
    11581 ctggcattca ctttacccga ctgcacatcg ctattgacag gtcgcaattg cgcataaaaa
    11641 gttaatggca ccttaccgct atgtaggctg tagtctttac tcgcctggtt aagcggaatc
    11701 aatatcttcg catcatccag aatcgcaatt gccagcccac tggctgcatc cgtaccactt
    11761 tcaatggaca gcatttcgct ctgcactccg tcgggggttc ctgaaaaggt gacatcaacc
    11821 gtgctgacat ctgttccgca atcctgcaaa ttaatgatga aagatttctc cgggccataa
    11881 ttcccatgaa aaaaacgtgt cagcgagaca tcgcctaact gcacattgat ttgcttgcta
    11941 tcctgtgcca gcacgcatgt attacggcga aacgatccgc taatgttgac cgtgccatca
    12001 tgggcaaata gctcacagcc ccccgttaat cccagtaaga acagaatcca gattatccgt
    12061 ttcaacatca ctccttcact ggtaaaccag atcaaaatag agaaccgccg tcgcattacc
    12121 gcccgttgct cccgcctttg tggacttata acgaagttga tatttgagtg tgttatcccc
    12181 ggcttttaag ggcgtaagag gctggacctg attgagcggg atttcttgct gactttgcgc
    12241 atcaagaatg cacggcaatc cccgttgcca taccaccgct tccgcctgtg tcagttagtt
    12301 tgagcagtgt cggcgcaagg gtgtcagcct cgccgctaaa aaggacgtcc gcgccagtaa
    12361 taccagcggc acaaccggtg atatcaatat tcaaatcagc agcagtgctt acggtattcg
    12421 cggcctgaaa atcactgata ttaaaatcac caatatgaat attttgtaat gcacttttgg
    12481 tggcgacatc acagccctga tcgaccacat tcccataaat atgcatactg acttgttcag
    12541 gcccagccat cccgacatgg atgttaagaa ggctaaagaa caaaataacg cactgatata
    12601 tcgttttttt cttcatacgt tcacgcgtta atcaaaattt attctcaccg ttgccgtggc
    12661 ttcaaatcgc ccggtttctg gtggtacgct tgtcgtgctg actggataga cattcattcg
    12721 cggatactgt ctgcatcatc aggaatagag agcggtagtg tcccaccagc aggggaaata
    12781 atgttgtgct ggctatcggt caccaccacg cccacgccag gacgtgacgt cttaatcgcc
    12841 tggggatagc tggggtctga ggttgccgtc agactcagat tgacgttaac taaccctgcg
    12901 tttcccgtgt tacacctgac cggaattgca agttctgccg ttctggcccc aagaggctta
    12961 tgccctacaa cacgaaagtt agccgcaaat acgtcgccca gatcaacctc gattgtttct
    13021 ccggcattga cggaacatga gccaagtgaa ttgatcgtac cagacaggta atacactaaa
    13081 accggtgtac cagtagtcgt gcaggaatca cctgcgggta tgttgtagca ttcaaataat
    13141 cgcgccagcg ccataggagg aatgacgacc gaaccaacga acggctgcaa tattttcagg
    13201 gaaaagctac cgctattacc gctagccccc tggccaccta aacgttgacc gccgggatcg
    13261 caaatatgcc atcgtgtatc tgcccctgtg cccacgtttt cgaaaggaac cgcagtagga
    13321 gaactgcctt taacctgcaa taaaactttg gcggcaagat agtcattaat tttgtaccag
    13381 ttgtcgccta aatagactaa cggtgaatcc gcggcgaatg cccatcggcc acttctgaca
    13441 ttgtctttat cgcaatcaca ggaaacataa taatcattcg attcgttcca ctgataaaat
    13501 ttgtctttaa ctactccagc cgtattttct tcttcatcca gagttttaac gccaaaattg
    13561 acgccaatat acggattccc ctgagtggta tgacactgcc cgatataagc agcagtttga
    13621 gcgcaccata gcattaagac ggcggcaaaa aatagtcgta gtaataacac gcatttttct
    13681 ccaaagatta tctgcatatg gcatccattc gcgtaatcgc agagttaatt tgttgttcgg
    13741 taagctggta actgatatga caaatttgat cgggagcttc gccccagcgt acagttatcg
    13801 ctccctgagc gggcaaaccg gacatataga gcgagccatc atcatcaata atgccgctat
    13861 tagtctgtat gccgtccagc gtcgctatcg cgccaaaacg cagcggtata ccctgctttg
    13921 atgttttcat taataccctt gctcccgcat gggcattgaa ctccgccaga accaacgccc
    13981 ctttggtggg aaccacgttg actactgcat tttcaagatc cacgttacgt tttaacgatg
    14041 ccgcatcaag tgcgattcga ttttcccgat aagaagtggc ataggggaca acggtatatc
    14101 cgcgccagtc agttttcagc ccggtgctgt tcgccacacg agtattctct gcgcctggtg
    14161 ctgcaatcag cacgttagtt tcccccagcg attgccccag ggtaatgccc tgtgaatggg
    14221 caactaaact gcctgaaaga gcgtagttca gttgttgttg actgccgtta tcgctgtagt
    14281 tgtagcccac tcgggcatct gcaaacgccc ctttgtagtc catgctggcg ctaccattag
    14341 ccgttttccc ctcgctgtta tatccctgtt gcacgctgta gctcaggtta ccgtcatcga
    14401 gtagtgtctc cgttaaaccc aggttttgtg taatgtgccc ttcgttatcg atactggtgt
    14461 tagaggtcat ccaggcgtaa ttttttttcc tggtatagct atcattactt ctggggagca
    14521 acagattgag cggcagggaa aaatttagtg caaacacctg atcagcatca ggttgaccac
    14581 gggacttact gtagttccag gaaaccccca gcgagagatc tttaatttgc gtgttgtagc
    14641 caaactgtaa tagccgatcc tgttgatcgg tatgccagta agtttgctgg ctaccactta
    14701 aataaaacga accgtactcg cctaattgct gggagatatt gacctgcagt ttgccacgtt
    14761 tggtgtaaaa caaattgtaa taacgcgacc acatcggcgt atcttcatca tcaccgtcat
    14821 taaattcgta gccatccata tgtttataca tggtgtcgga aagggtgtag aaacccgacg
    14881 tcgaatagcg atagcccatc aattgaaaat tagtgcccaa ttggttgagc gttttggcat
    14941 acagaaaacg atatgactga cctttatgtt cgctttggtc tgccagcgta ctcttcgcct
    15001 gggtcgcatc gaacgaaatt gctccgaaat cgccacggtt aaatcccaga ccaaacatgg
    15061 cggcacggta atattcagca tattgtccac caccatacca tgtcgttccc cacggtccgc
    15121 cccactgcaa cgtggtctgg gcaaatttgc tctcctgctg ttcattacta ttggttctgt
    15181 atttcgccag tgtcaccgcg tatttgattc gcccctgacg ctggagtaat ggcacgctgg
    15241 aaaacggtac gctgtagcta ttgacgctgc cgtccgcttc tttgatttca actaacaaat
    15301 caccgctcga cgacgtggaa tagagatcac taatttcaaa agcaccaggc gatacataag
    15361 tttggtaaat ggtgtaacca ttttgcttaa tcgttatctg ggcattgctt ttggcgatac
    15421 cacgcaccac aggcgcaaac cctttcaggc tgtctggcag catgttgtca tctgacgcca
    15481 gctgcacacc acgaaagctg acactatcga aaaaatcgcc tgcagtataa tcatcaccta
    15541 cggtcagttc gccttttagt ggaataaccg cccgctgcaa atagctgctg agattcttcc
    15601 attctgcggt ttggccacta ctgcgactcc atgttgaatt gttgcgtaat ctccatgggc
    15661 ctaaattaac gccactgttt aaattcagaa aatagctgtc gccagaatca ctgtctgcgc
    15721 tgctatgaat actgttagcc ccgctaaacg aatatcccag taatagcgcg ttgatccctt
    15781 catcccaacg ttcagggggg acataaccac gggcattact tttgatggcg atttgcggta
    15841 ctgaaatttc cagacggagt ttattcacat taaacgttac gctggcatcc ggaataatct
    15901 ccgccaatgg cacacacact tgtttgtcca gttgtttaaa agccggaaac gcatcagtat
    15961 taacacccat tgcatccagg ctttcagtcg tcaaacaggc ggcaatcccc ttctctggcg
    16021 ttatctcaac aaaattgacg ttgcgcgtat ctacaattgt ctgattcaga acaatatcga
    16081 cacgataaac ccctggcgtt attttttgtc ctttttcgaa acgagacaaa tcggccacca
    16141 ttgagggatc atctttgaaa aaagcaggat cgaaccaggt ttcagcttgt gcattgaacg
    16201 ctgccgacac aaacaccacc agcccaccaa taaacgatgg cactccacca gagcttaaca
    16261 ggctgtgttg tcgatgagtt ctatacatct cgctctctta actgaagacg gcttacgatt
    16321 tacagttgag ttgcggcgtt tccgccccgt agtcattgat ggttcgata
//
LOCUS       DAZXUK010000072        16318 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZXUK010000072 DAZXUK010000000
VERSION     DAZXUK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16318
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.072"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACH3L8_001830"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACH3L8_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
     gene            complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACH3L8_001831"
     CDS             complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACH3L8_001831"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFT4084972.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACH3L8_001832"
     CDS             complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACH3L8_001832"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFT4084973.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACH3L8_001833"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACH3L8_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="ACH3L8_001834"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="ACH3L8_001834"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFT4084974.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="ACH3L8_001835"
     CDS             complement(5877..6218)
                     /locus_tag="ACH3L8_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFT4084975.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACH3L8_001836"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACH3L8_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFT4084976.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACH3L8_001837"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACH3L8_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFT4084977.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="ACH3L8_001838"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="ACH3L8_001838"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFT4084978.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="ACH3L8_001839"
     CDS             12897..14240
                     /locus_tag="ACH3L8_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFT4084979.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="ACH3L8_001840"
     CDS             complement(14302..14508)
                     /locus_tag="ACH3L8_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFT4084980.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="ACH3L8_001841"
     CDS             14833..15387
                     /locus_tag="ACH3L8_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFT4084981.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..>16318)
                     /locus_tag="ACH3L8_001842"
     CDS             complement(15450..>16318)
                     /locus_tag="ACH3L8_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4084982.1"
                     /translation="THSNDFFNNIQHAFKKKDWVSNYDSLLTLRESFRCATQIDKNSY
                     QVLSSKNETVNAMDNFLISFSLKDNGAEYTITLRGSGFEYEEIPITINEYNSFMDFKN
                     REFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRGKF
                     ELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLEDED
                     YSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSTIEAFKKEQLA
                     LPVLVRCKNSET"
BASE COUNT         3902 a         4440 c         3980 g         3996 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aattgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttt
//
LOCUS       DAZXUK010000073        16312 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZXUK010000073 DAZXUK010000000
VERSION     DAZXUK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16312
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.073"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(36..3317)
                     /locus_tag="ACH3L8_001843"
                     /pseudo
     CDS             complement(36..3317)
                     /locus_tag="ACH3L8_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            3435..4628
                     /locus_tag="ACH3L8_001844"
     CDS             3435..4628
                     /locus_tag="ACH3L8_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFT4084983.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4826..6079)
                     /gene="glyA"
                     /locus_tag="ACH3L8_001845"
     CDS             complement(4826..6079)
                     /gene="glyA"
                     /locus_tag="ACH3L8_001845"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFT4084984.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6406..7596
                     /gene="hmpA"
                     /locus_tag="ACH3L8_001846"
     CDS             6406..7596
                     /gene="hmpA"
                     /locus_tag="ACH3L8_001846"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFT4084985.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7641..7979)
                     /gene="glnB"
                     /locus_tag="ACH3L8_001847"
     CDS             complement(7641..7979)
                     /gene="glnB"
                     /locus_tag="ACH3L8_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFT4084986.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(8040..9374)
                     /gene="glrR"
                     /locus_tag="ACH3L8_001848"
     CDS             complement(8040..9374)
                     /gene="glrR"
                     /locus_tag="ACH3L8_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFT4084987.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9364..10077)
                     /gene="qseG"
                     /locus_tag="ACH3L8_001849"
     CDS             complement(9364..10077)
                     /gene="qseG"
                     /locus_tag="ACH3L8_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFT4084988.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10242..11669)
                     /gene="qseE"
                     /locus_tag="ACH3L8_001850"
     CDS             complement(10242..11669)
                     /gene="qseE"
                     /locus_tag="ACH3L8_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFT4084989.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12227..16114)
                     /gene="purL"
                     /locus_tag="ACH3L8_001851"
                     /gene_synonym="purI"
     CDS             complement(12227..16114)
                     /gene="purL"
                     /locus_tag="ACH3L8_001851"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFT4084990.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
BASE COUNT         3834 a         4454 c         4214 g         3810 t
ORIGIN      
        1 agcgcgtgcc gctcaaatat tacagttaat agggtttagt gaacgtagtt aaaaataaaa
       61 ggcatcacag tggtgaataa tgccgctttc ggccaggccg gattaatcgc cgtcaattca
      121 gcgcgagcag actcatattg cgcaacctcc ccgagtcccg cggacgccag catcgccacc
      181 atcaggcatt tttctttatg ctgctgttgt aaatcgccat acagcgataa caggtcaggt
      241 tgtgaaacag caaagaaatc cgcctcaata ctggttttcg ccatctcttg cgcccactgt
      301 ttcatttcac taaacagttg ctgtgcggtt tgctgttcac ccagcagtcg cagcgccatt
      361 ccttgccaga agagataatc aaccggctga tcgttgtaat aactgtggat gttaatggtg
      421 cgatcgccgg tcgccgccag acgtaaacaa cgcatcgctt cagtttcatc gccctgcgcg
      481 ttggcgcata tcgcctgcca gaaccagatg tcgttatcag tttgccccgg taaacggcct
      541 tcgcttaaat tctccggata atacagcgcg gcatgaagca gttcgctggc ctgctgcggc
      601 tctctggcat caagatgctg ccaggcgcgt aataactggt tgaggataaa ctgactggtg
      661 accttccctt ccccgccttc ccacgggtgg aatttacgcg tggcgagaat gtccgccgct
      721 ttgtctgcct gacccgtgag atgccacaaa ttgagcagtt ctgcggtcat gtcgtcgcgt
      781 ttcagcgcaa tttccagatt attttccaga cgcgccagtc gtttctccgg tgtggctcca
      841 cttaacttat ccagcaaatc ccgttcgaaa agcagacgtg catcctgcgg cgcaagctga
      901 taagcattat caagataacg cgcggccagc tcataatcgt gtttcttatt ccacgcatgg
      961 atcgctaacc cgcgccagcc gtcggcaaac tccggcgaca tctctacgca acgttgccat
     1021 aaggcaatgg ctttgttgta gctacgtttg ttgtagtaga agcaggccag taaatggcga
     1081 gcaaaccaac actcttcaat actctccagc gccgccactt cttccagcgt attcgggaaa
     1141 cggacaaact gcgggaagac atcaatggct tttgcgacca gttcgccacg ttcggctttc
     1201 ggcagcaagc tggcttgcag gtaaagcggc agcgtgcgct ggcagtccag tgcgttcagc
     1261 atctctgccg ccagggtggg cattccccag ttaatcagtt gcccggcggt catcagagcg
     1321 ttaacgtcgc gtccctgaca cagaccgcgc cactgcgcga gggctgattc gctacgacca
     1381 tcgaaccagt tcagccacca cagagtggcg ttcagcggat aatcacgcag cagtttctcg
     1441 cgctgcaaac gcgcgttgtc ctgacgacca cttaacacca gcagcagatt atgcaagcaa
     1501 agcacttcct gattggttgg gcaggcacga agactttgtt ggcaaaaatc cagacccgcg
     1561 tcgaagttat cattacgcgc cgccagtcgt gccagaccat aatagccgcc tgctttacta
     1621 ttgccgctcc agaccgcacg ccagaaatcc tcttcggctt gttgatattg tccctgacgt
     1681 tcgtaagcac tggcgcgaat caaacttgct tgtccacact gcggtttttt gttcagcgca
     1741 tgtgcacgtt tcagcgcctg actggcatac gccaccgctt gcgggaaatc tgcgcggtta
     1801 tattccagca tcgccagcgc caagttacag cgataatcca gcgggtccag cgccacgccg
     1861 cgcaggtagt aatcgaacgg tgaacggctg gcgtgatgat attgctccag atgctgaccg
     1921 ataaaccagg cttcatctgt actggtaatg tcttgtgctg ccagtggcgc tttggcgacg
     1981 tccggcaacg gcaactcctg cggctgatgt tcctgataac tcagtacaat attgccgtcg
     2041 gcatcggaga gttcaatggt cagtctgtca ggattgatac cgtgcaacac gccctggatg
     2101 gcggtcgctg gtgtcagggc cacggcatca tcgagcaacg cgttgcattt gccgatttcg
     2161 cggatcgcca gacggtatcc gttcaacgga gagatggcat acagccccca ctcaatcccc
     2221 cgctcactac gctggagttt tatcaccgca tcgcgggagg cattttgcac catgcccaga
     2281 gaatgataag gcaggaaata ctgctcgaaa cgcttctctt cgtaagcatc aagccaggta
     2341 aaatcaggct ggttatcggc aaaaataccg gtcatcagtt cgatatacgg gccgttattg
     2401 tcggtcagac tcttatccca cgcctggcca aattcactgt gtccccaact ccactgtttt
     2461 ttacctggcg caatatggtg gttggcaacg tgcagcaaac cgccatcttc atcgtgacac
     2521 cacgcgccga caaaatcgta ctgcgatttt tcggccatat atgaggttgg aacaggcaca
     2581 tttttatagc gagaaatgtc cactccggcg gagtagtcca ctttgtagta agtgccggtg
     2641 gcgatgggga aagcggagac ggcccgtttg ccgtgatcaa acaccgcagt cacatccggt
     2701 gggaaaacgc tctgatgccc ttcaccccct ttcactgccg ggttgaccca ccacaagaaa
     2761 tgacgcggcg tggcgttgcc gttatagacg cggctggcga tttccagtgc cgcccggtca
     2821 gggcgcaggg tgaaacctgt catcacctgt aaaccatgca tcggctccgt ttcgccgacc
     2881 cacaccgtct gtgcaccgtc ttcatgggct tcgagggtga aatcaacggg cataaaggtg
     2941 gtcgggcgat ggtgttgcgg ccagttaaac tcaatcccgc cggagatcca cggtcccagc
     3001 atccccacca gcgcaggttt aatgacttca ttgtgataga caaaatcgcg ctgtttcact
     3061 ttatcccatg cgcgatgcac ccgaccgccc agttccggca ggatcatcac tttgatgtag
     3121 tcgttttcca gccacaccgc ctgctaggat ttcagggttt tctgctcgct cagcgtatcg
     3181 gtcacgccgt agggataaac cgcgccggac gatccctgat aaacgcgatt ttccaggaac
     3241 atgggatgta tatcctgcgg cccggtttca taggtcggga tctcaacgcg ctcttgccac
     3301 acttttactg gagtcatggt gccctcaata ttaacaagac atactgaatt aaaagatttg
     3361 tggcagtgta ttgaacaatc tggcaatgtt ttcgcggaat aatcacgcaa ttaactaaac
     3421 aaggtttagt gaagatgaga gcctgcatta acaatcaaca gattcgccac cataacaaat
     3481 gcgtgattct ggaactgctg taccggcaaa agcgcgccaa taaatcaacg ctggcccggc
     3541 tggcgcaaat ttcgattccg gcagtcagta atattttgca ggaactggaa agcgaaaaac
     3601 gggtggtgaa tattgacgat gaaagccaga cgcgcgggca tagtagcggt acatggctga
     3661 ttgcgccgga aggtgactgg acgctgtgcc tgaacgtgac gcccaccagt attgagtgtc
     3721 aggttgctaa tgcttgttta agtccgaaag gcgaatttga gtatttacag attgatgtac
     3781 cgacgccgca ggcgctgctg tccgaaatcg aaaaatgctg gcatcgccac cgtaaattgt
     3841 ggccggaccg caccatcaat ctggcgctgg caatccacgg gcaggttgat ccggtgaccg
     3901 gcgtatcgca aaccatgccg caagcgccgt ggacaacgcc agttgaggtg aagtatctgc
     3961 tggaagagaa gctgggcatt cgggtgctgg tcgataatga ctgcgtgatg ctggcgctgg
     4021 cggagaaatg gcaaaataat tcgcaggaac gggatttctg cgtgatcaac gttgattacg
     4081 gcattggctc gtcgttcgtg attaacgagc aaatttatcg cggcagtttg tatggtagcg
     4141 gacagattgg tcacaccatc gttaatccgg atggagtcgt ctgcgactgt ggacgttacg
     4201 gcagcctgga aaccgtcgcc tcgttaagtg cattaaaaaa acaggcgcgg gtatggctaa
     4261 aatcacaacc ggttaatact cagcttgagc ctgaaaaact gacaaccgcg cagttaatcg
     4321 ctgcctggca gagtggagaa ccgtggatca ccagctgggt tgatcgctct gccaatgcca
     4381 ttggtttgag tctgtataac ttcctcaaca tcctcaatat taatcagatt tggttgtacg
     4441 gtcgcagttg tgcctttggt gagaactggc ttaatactat tattcgccag acaggattta
     4501 acccgttcga ccgcgacgaa ggaccgagcg tgaaagcgac gcaaattggc ttcgggcaat
     4561 taagccgcgc acaacaggtg ctgggaattg gctatttgta tgttgaggcg cagttacgac
     4621 agatttgatg gcgcgataac gtagaaaggc ttcccgaagg aagccttgat gatcataaac
     4681 gaaaaattgc ctgatgcgct acgcttatca ggcctacacg gagattgcaa tatattgaat
     4741 ttgcaagatt ttgtaggcct gagaaggtgt tcatgccgca tccaacatga acaacgagca
     4801 cattgtcagc aaatcaccgt ttcgcttatg cgtaaaccgg gtaacgtgcg cagatgtcga
     4861 gaactttacc tttgatgcgc tcgataacgg cttcatcatt gatgctgtcc agcacgtcac
     4921 acatccagcc agccagttct ttcgcttcgg cttctttaaa gccgcgacgg gtaatcgccg
     4981 gagtacctac acgaataccg gaggtcacaa acgggctctt cggatcgttc ggtacgctgt
     5041 ttttgttgac ggtgatgtta gcacggccca gagcggcgtc tgcttcttta ccggtcaggt
     5101 ttttatcaac cagatcaacc aggaacaggt ggttatcagt gccgccggaa accactttgt
     5161 agccgcgctc gaggaacact tctaccatcg ctttagcgtt tttcgcgacc tgctgctggt
     5221 aagttttgaa ctcaggctcc atcgcttctt tcagagcaac cgctttaccg gcgattacgt
     5281 gcatcaacgg accgccctga ccaccaggga aaacggcaga gttcagtttt ttgtacagct
     5341 cttcgctacc acctttcgcc aagatcaggc cgccgcgcgg acccgccagg gttttgtgag
     5401 tggtggtagt aacaacgtga gcatgaggaa ccgggttcgg gtagacgcca gcagcaacca
     5461 ggcccgcaac gtgcgccata tcaacgaaca gataagcacc gatgctgtca gcgatttcac
     5521 gcattttcgc ccagtcaacc acgccggaat atgcagagaa accaccgata atcattttcg
     5581 gcttgtgttc tttggcttgt ttttccagat cggcgtaatc gatatgaccg gtagcatcga
     5641 taccgtaagg aacgatgttg tacagtttac cggagaagtt aaccggagaa ccgtgagtca
     5701 ggtgaccgcc atgcgccagg ttcataccca gaacggtatc acctggttcc agcagcgcgg
     5761 tgtagaccgc aaagttagcc tgggagccgg agtgcggctg gacgttagcg tagtcagcgc
     5821 cgaacagttc tttcgcacga tcgatcgcca gttgttcaac gatatcaaca tactcgcaac
     5881 cgccgtagta gcgtttgccc ggataacctt cagcatattt gttggtcagc tgagaaccct
     5941 gcgcctgcat tacgcgcggg ctggtgtagt tttcggaggc gatcagttcg atgtgctctt
     6001 cctgacgtac tttttcctgc tccatagcct gccacagttc ggcatcataa tcggcaatgt
     6061 tcatttcacg ctttaacatc cgcatctcct gactcagcta acaataaaat ttttggcctt
     6121 tataggcggt cctgttggac aacggcgaac agtataaccg aatcattgtg cgataacagg
     6181 tcttgacaaa ggaatttacg caaacgatta ccttcaggct acgcaaggct ttggagaata
     6241 aagagcttgc aaccggaaac ggatttcttt tcaggtttgt gatgcaaatt tttcacttca
     6301 tcacattctt tctgaaaaac accaaagaac catttacatt gcagggctat tttttataag
     6361 atgcatttga gatacatcaa ttaagatgca aaaaaggaag accatatgct tgacgctcaa
     6421 accatcgcta cagtaaaagc caccatccct ttactggtgg aaacggggcc aaagttaacc
     6481 gcccatttct acgaccgtat gtttactcat aacccagaac tcaaagaaat ttttaacatg
     6541 agtaaccagc gtaatggcga tcaacgtgaa gccctgttta acgctattgc cgcctacgcc
     6601 agtaatattg aaaacctgcc tgcgctgctg ccagcggtag aaaaaatcgc gcagaagcac
     6661 accagcttcc agatcaaacc ggaacagtac aacatcgtcg gtgaacacct gttggcaacg
     6721 ctggacgaaa tgttcagccc ggggcaggaa gtgctggacg cgtggggtaa agcctatggt
     6781 gtactggcta atgtatttat caatcgcgag gcggaaatct ataacgaaaa cgccagcaaa
     6841 gccggtggtt gggaaggtac tcgcgatttc cgcattgtgg ctaaaacacc gcgcagcgcg
     6901 cttatcacca gcttcgaact ggagccggtc gacggtggcg cagtggcaga ataccgtccg
     6961 gggcaatatc tcggcgtctg gctgaagccg gaaggtttcc cgcatcagga aattcgtcag
     7021 tactctttga ctcgcaaacc ggatggcaaa ggctatcgta tcgcggtgaa acgcgaagag
     7081 ggtgggcagg tatccaactg gttgcacaat cacgccaatg ttggcgatgt cgtgaaactg
     7141 gtcgctccgg caggtgattt ctttatggct gtcgcagatg acacaccagt gacgttaatc
     7201 tctaccggtg ttggtcaaac gccaatgctg gcaatgctcg acacgctggc aaaagcaggc
     7261 cacacagcac aagtgaactg gttccatgcg gcagaaaatg gcgaagttca cgcctttgct
     7321 gatgaagtta aggaactggg gcagtcactg ccgcgcttta ccgcgcacac ctggtatcgt
     7381 cagccgagcg aagccgatcg cgctaaaggt cagtttgata gcgaaggtct gatggatttg
     7441 agcaaactgg aaggtgcgtt cagcgatccg acaatgcagt tctatctctg cggcccggtt
     7501 ggcttcatgc agtttaccgc gaaacagtta gtggatctgg gcgtgaagca ggaaaacatt
     7561 cattacgaat gctttggccc gcataaggtg ctgtaatttg atgttgccgg atggaaacat
     7621 ccggcaaccc ttgacgcggt ttaaattgcc gcgtcgtcct cttcaccggt acggatgcga
     7681 atgacccgag ccacgtcaaa gacgaagatt ttaccgtcac cgattttgcc ggtttgcgcc
     7741 gtgcgaataa tggtatcgac acaggtatcg acaatgtcgt ccggtacgac aatctcaatt
     7801 ttcactttcg gcagaaaatc caccatatac tccgcgccgc ggtacagctc ggtatggcct
     7861 ttctggcgac caaagccttt cacttcggtc accgtcatgc cggtaatacc gacttcggcc
     7921 agtgcttcgc ggacatcgtc cagcttgaag ggttttataa tcgcatcaat ctttttcatg
     7981 ctattccttg aaaaggttgc ctgtcttttg atctgctaaa cgtaacacat aacgccaatt
     8041 cattccttga aatcgtttgc atccagctcg tgtcgggaaa gcagtttata aaattctgtc
     8101 cggttgcgcc ccgccattct cgccgcgtgg gtgacgttgc ctttggtgat ttgcagcagc
     8161 ttacgcaagt agttgagttc aaaatgatta cgtgcctcaa caaaggttgg cagcgccgta
     8221 ttttcaccct ccagcgcctg ctccaccagc gcatcactaa tcaccggaga tgaggtcagc
     8281 gccacgcact gttcaatcac gttgaccaac tggcgcacat tacccggcca gctcgcggtc
     8341 atcaggcgtt tcatcgcatc ggtagagaac gcgcggacaa acggtttatg tcgctctgcc
     8401 gcctggcgca acaggtgatt tgccagtagc ggaatgtctt ctgtgcgctc cgccagtgcc
     8461 ggaattttca ggctgacaac gttgaggcgg taatagagat cttcgcggaa ttccccgcgc
     8521 gccatcgctt ttgacagatc acggtgagtg gcagaaataa tccgcacatt gatatcaata
     8581 tcgcggttac tgcccagcgg gcgcacttta cgctcctgca acacgcgcag cagtttgacc
     8641 tgtaacggcg caggcatatc gccaatttca tcgagaaata gcgttccgcc ttccgccgcc
     8701 tggaataaac cttcgcgatt gctgacagcg ccagtaaacg cgccacgcgc atgaccaaac
     8761 agctccgact ccagcaattg ctcgggcaat gcgccacagt taatagcaat aaatggtttg
     8821 ctgttgcgcg ggctggcgtt gtggatagcc tgggcgaaaa tctctttccc ggtgccgctc
     8881 tgaccgttaa tcaaaacgct gacgtctgat tgcgccacca gccgcgcctg ttccagcaaa
     8941 cgcagcatca gcgggctgcg ggtgacaatt gcctcgcgcc agcgttcatc ggtggctggc
     9001 gcggattgct ccagcgcatc gtcaattgcc tgatatagcg catctttatc gacaggcttg
     9061 gtgaggaaac taaaaacgcc ctgctgtgtt gcagcaacgg catcggggat agaaccatgc
     9121 gcagtaagaa taattactgg cattcccggc tgcactttct ggatttcagc aaatagctgc
     9181 ataccgtcca tttcatccat ccgcaggtcg ctgataacta aatctacttt ttcgcgattc
     9241 agtacccgta atccttcagc gccactttcc gccgtgacca cactgtagcc ttcgctggtc
     9301 aggcgcaggc caagcagttt cagcaatccc ggatcgtcat cgaccaataa taaatgcgca
     9361 ggtttatggc tcatcaggag tgacctcatg ggtggatggc gcgggctttt cactctcgtg
     9421 cggcgtatcc ggcgagaaat ttccagccgg tttgcgggtc gagagctggc gttcaatatc
     9481 ggtcaggttt tccagcttgc gggtggtgag ttccagttgc tgctgtagaa cgtgatgttg
     9541 ctggcgcaat gtatccagct cgctgtcgct ggactgctgg agtttactgt aacgttggcg
     9601 ctcttccgcc agttgcagtt gcagcgcctg accgtcgcgc cagagttgat acagtgggcg
     9661 aacctgtgcc gggatctcgg tacttaacgc ttcaatacgc gcgaccagct ggcggcgctc
     9721 atacggcgta attttggcgt cggcgagcaa aatcccttgt ttaaaggtat tttgccagct
     9781 gccgtcgtca tattggcgag cttgctgacg cgactgcgca ggcattaaac gatcagcaca
     9841 atccatcgcc cgcagccagt aaagcggatt ggtttcggtt gatttacctt gcagcgccca
     9901 gatgtcgcta cattcagtag aaagatagtc agccagttga taaaccggaa ttttttcttc
     9961 tgctggcgta tcaatggctg gcttattgtg attctgcacg caacccagca atgccagaca
    10021 tggcagccct gccagccaca gccgtcgggg caataatcgt tgaaaaatgt gtcgcatatt
    10081 caccagactt aaagcctatc ccagtgggcg taattgttgc agacagtctg gacatggaca
    10141 gcgcggagaa accggggcgt acatatcgta cgtgaagatt tcgagcacta cccggggccg
    10201 aactgacaaa taaaatagcc tgataggata ggctgttagc attatttcgt gtttttcgac
    10261 gacggtaatt caatgcggaa acaaacgtct tgcccgctct cgtcgaccag atacagttcc
    10321 ccttgcatac ggcgaataca atccctggca atgcttaatc ccagaccgct gcccttcacc
    10381 gccccttttc gctggtggct tccctgaaaa aagggttcga agatcatggc gcgttcctct
    10441 tgcggaatgg gcgtgcctgt attgatgaca tcaatataaa cccgcgcacc atgtaaactg
    10501 ctgcgaaggc aaatgttacc ggattcagcc ccgtagtgca ccgcattgga gtaaagatta
    10561 tccagtacgc tcatcagcag cattggctcc gccaggcaag ctgttgcttt gagatcgacg
    10621 tcggtatgca tcattttagc ccgtgcgggc aggctatgag cagaaaccac tgtctccacc
    10681 agcggtgcta attcaacatt ctccagttcc accgcactgt ccgcctgttt acggttgtaa
    10741 tcaagcagtt gttcgatcag tttttgcaaa ttgcagctgc tgctatcaag aatgctcacc
    10801 acctcttttt gctctggcgt aagcggcccg acaacctgat cagccagtaa ttcagtgccc
    10861 tcgcgcatac tcgccagtgg cgtttttaat tcatgagata aatgtcttaa aaattgatgg
    10921 cgttgggatt ccagccatga caggcgctca cttaaccaaa gaatacgttg cccaaccgag
    10981 cgtaactcgc tcggtccaca gaacgaaacg ctattgccca gagaacgccc ttcccccaga
    11041 cggttgatca tgcgctcgat atttttcacc ggcccgataa tcatccgcgt gaaaagtagc
    11101 accatcacca gactcaccag aaatagcacc agcgattgcc aaccaaaata ttgcccacgt
    11161 tcggcgattt cacgctgaag ttgctgccca cgagagaaca ccactgtgcg cgtggcctgc
    11221 accatttcgg tattggcact ggcaaaggct tcaagacgcg cggcggcggc agcatcggga
    11281 ccactgttgt tacactgaag ttgggccaga ttgttcaaat cctgacgcaa tgcctggtaa
    11341 agtttatcgt ctggcagtac gcctgcgtgg gcatcgagca tttcgctgta acgcttgcgc
    11401 tggctttgat aaaccttcgc aagcgtaggg tcgtccagca cgcaatactg acggtagcta
    11461 cgctccatct ccagcgccgc gttggtcatc gcttcactac gccgggcatc aataagcgta
    11521 gtgcggttaa ccagcgccgc ctgatcgcta agcgcattca ggctttgcca ggcttgccat
    11581 gccagcacca acaggggtag tagaattagc aaaaatgcca gcattaccag ttgtcgtaat
    11641 gagcggggaa aaacgggcca gcgtttcaag gtgttactct cgtcagacgc gaatagcctg
    11701 atgctaaccg aggggaagtt cagatacaac aaagccggga attacccggc tttgttatgg
    11761 aataaggcgg tgcctaactc gacgtttcgc ccgatggttg atatagctac gctgatatca
    11821 gaagttggac ggcaggcacc ttgttgtgcg tcattcgaat tttatgtagc acgtcccgaa
    11881 ggggctgaca taagtcggtg aatgagccac tgcttactat tatgcagtaa ctgtgccaat
    11941 aaagaaaata gtttggtaac gtactgatta tacgttgctt tgagggattt atggcttctt
    12001 cgctatttga atgtaaatca gccactgtgt cgctaaaaag agacaactta agataaactt
    12061 attagattac aataaaatca atcatttaag tgtcgccaga aagcgacacg gtaaaccacc
    12121 cattgtcgtg atttacagac acaaaaaagc tcccggagtt gggagcttat gatagtggtt
    12181 ggtgcttaat gccgcatccg ggctgcaaaa ccaatgaact gatgacttac cccaactgct
    12241 tacgcgcatt gcggaaaatg cgcatccatg ggccatcctc gccccagttt tccggatgcc
    12301 aggagttgct gacagtacgg aaaacacgtt ccgggtgcgg catcataatg gtgactcgac
    12361 cactttcagt cgtaaccgcc gtaataccgt tcggtgaacc gttcgggtta gccgggtagg
    12421 tttcagtgac tttgccgaag ttatcgacat agcgcagtgc caccagccct ttgctttcca
    12481 gtgccgccag atgcgccgca tcacgcactt ccacgcgccc ttcaccatga gagacagcaa
    12541 tcggcatttg cgagcccacc atcccctgca acagcagaga cgggctttgg gttacttcaa
    12601 ccaggctgaa acgcgcttca aagcgatcgg aggtgttacg cacaaaacgt ggccacaact
    12661 cactccccgg gatcagttca cgcaggttag acatcatctg gcaaccgtta cataccccca
    12721 gggccagcgt ttgcggacga tggaagaagg ttgcaaactc atcgcgtaca cggtcattga
    12781 acaggataga cttcgcccaa ccttcaccgg cacccagcac atcaccgtag gagaaacctc
    12841 cgcacgcgac cagggcgtga aaatcctcca gtcccgtacg tccagtcagc aggtcgctca
    12901 tatgcacgtc gatagcatca aagcccccac gttggaaagc tgccgccatt tcaacatgcg
    12961 agttcacgcc ctgctcacgc agtacggcaa ctttcggacg tgcgccagtg gcaatatacg
    13021 gtgctgccac atcttcgttg atatcgaacg acagttttac attcaggccc ggatcggcgt
    13081 cgttagattt cgcctgatgc tcctgatcgg cacactccgg gttgtcacgc aggcgctgca
    13141 tctgccaggt agtttctgcc caccagacac gcaacgtagt gcggctttcg ctgaatacag
    13201 tctgcccgtt ggcggtaatc acaaaacggt caccggaaac cgcctgccca acataatgga
    13261 cgcaatccgc aagcccatgc tgtgccagta cggactcgac cgcttcacgg tcagcggcac
    13321 gaacctgaat caccgcaccc agttcttcgt taaacaacgc cgccaggcga tcgtcaccca
    13381 gagaggcgat atccgcgtca atgccacaat gacctgcaaa ggccatttcc gccagcgtta
    13441 ccagcaggcc tccatcagag cggtcgtgat acgccagcag cttacgctgt gcaaccagcg
    13501 cctgaatcgc gtcatagaag cctttcagtt ggacaacatc gcgtacatct gccggtttgt
    13561 cgccaagctg acgataaacc tgcgccagcg ccgttgcgcc cagcgcgtta ttgcctttgc
    13621 ccaaatcaat cagcagcagt gcgttatctt cggtacaaag ctgcggcgtg atggtgtgac
    13681 gtacatcttc cacgcgggca aatgcagaaa tcaccagcga cagcggcgac gtcatttcgc
    13741 gctcttcgtt accttcctgc cagcgggttt tcatcgacat agagtcttta cccaccggga
    13801 tcgtcaggcc cagcgccgga caaagttctt cacccacggc tttaacggct tcatacaggc
    13861 ccgcatcttc accagggtgg cctgccgccg ccatccagtt ggcggaaagt ttaatgcgtt
    13921 tgatatcgcc aatctgtgtt gcggcaatat tggttaacgc ttcaccgacc gccagacggg
    13981 cagaggcggc gaaatccagc agcgcaaccg gcgcacgctc gccaatcgcc atcgcttcac
    14041 cgtagtagct gtcgaggctg gcggtagtga ccgcgcagtt agcgaccggc acctgccacg
    14101 gccccaccat ctgatcgcgc gccaccatgc cggttacgct gcggtcaccg atggtcacca
    14161 ggaaagtttt ctccgccacg gtcggcagat gcagtacgcg tttcaccgcg tctgcaatgg
    14221 tgatcccttc acggaccagc gcatcgccct tcgctttcag cgtttgtaca tcgcgggtca
    14281 tcttcggcgt tttaccaagc aggacgtcca gcggcagatc gatcggctga ttatcaaaat
    14341 gacgatcgtg cagagaaaga tgcagttctt cggtcgcttc accaatcacc gcgtagggtg
    14401 cgcgctcacg cttacacagt tcgtcaaaca gcggtaattg atcggcagca accgccagca
    14461 cgtagcgttc ctgggattcg ttacaccaga tttccagcgg gctcatgccc ggttcgtcgc
    14521 ttagaatatc gcgcagttca aatttaccgc cgcgtccgcc gtcgctcacc agctccggca
    14581 tcgcgttaga aagaccgccc gcaccgacgt cgtggataaa cagaattggg ttagcatcac
    14641 caagctgcca gcaacggtcg atcacttcct ggcaacgacg ctccatctcc gggttgtcgc
    14701 gctgtacgga agcaaagtcg aggtcggcat cagactgacc agacgccatg gaagacgccg
    14761 cgccgccgcc aagaccgatg ttcattgccg ggccgccgag aacgaccagc ttcgcaccga
    14821 cgttgatctc gcctttttgt acgtgatcgg cgcgaatgtt gccgatcccg cccgccagca
    14881 tgatcggttt gtgataaccg cgcagctctt cgccattgtg gctgttcact ttttcttcat
    14941 aagtacggaa gtagccgttc agtgccggac gaccaaattc gttgttaaac gccgcaccgc
    15001 ccagcgggcc ttcggtcatg atgtccagcg cggtaacaat gcgctcaggc ttaccgaaat
    15061 cttcttccca cggctgttcg aagccaggaa ttcgcaggtt ggatacggag aaaccaacca
    15121 aaccagcttt cggcttcgcg ccgcgcccgg tggcaccttc atcgcggatt tcaccgccgg
    15181 aaccggtcgc cgcgcccggc cacggagaaa tcgccgtcgg gtggttgtga gtttcgactt
    15241 tcatcaggat gtgcgccggt tcctgatgga aattgtagcg gcccgtttcg tggtcagcaa
    15301 agtagcggcc cacttcagaa ccttccatta cggcggcgtt atctttataa gcagagagaa
    15361 cgtgatctgg cgtggtttcg aaagtatttt tgatcatcct gaacagcgat ttcggctgtt
    15421 gttcaccgtc gataatccag tcggcgttaa aaattttgtg gcggcagtgc tcagagttcg
    15481 cctgagcgaa catatacagc tcgatgtcgt tcgggttacg accaagcttt gtgaaagcat
    15541 cctgcagata gtcaatttca tcttccgcca gagccaagcc aagacgcagg ttagcgtcga
    15601 tcagcgcctg acggccctgc cccagcaaat caacgcttgt gaccggagtc ggttgatggt
    15661 gggcaaacaa ctgctctgca tcatctaaat caaaaaagac cgtttccatc atgcggtcgt
    15721 gcagttcagc ggtaacctgc tgccattgtt cattggtcag cgtaccggct tctatatagt
    15781 aagcaacgcc gcgctcaagg cggtttacct gttgtagccc gcagttatgg gcaatatcgg
    15841 tcgctttcga agaccaggga gagatggtgc caggacgcgg ggtcaccagc aggagtttgc
    15901 cttgcggggc gtggctggcg agtgccggac catatttcag caggcgttca agttgtgcgt
    15961 gctcatcatc gtttaacggc gcattgaggt cagcaaaatg gacatactcg gcgtaaatat
    16021 tgtgaaccgg gagcctggca gcctgaaaac gtgccagcag tttgttgatt cggaatgccg
    16081 acagtgcagg cgaaccacgc agaatttcca tcataagtct ctcgtcttct aagctttcgg
    16141 tgtacccaag gggggggaaa cgggcgtcat tataaagaat ctgatgcgct gacgaaaccg
    16201 tttgcgtgga aataaaatca ccatcgtgaa ttagcaacgc gtgccgccaa tggctgtaat
    16261 aagttgccat ctggcgcagg tttacgcaaa atgccgctca tttatgagta aa
//
LOCUS       DAZXUK010000074        16096 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZXUK010000074 DAZXUK010000000
VERSION     DAZXUK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16096
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.074"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..588
                     /locus_tag="ACH3L8_001852"
     CDS             <1..588
                     /locus_tag="ACH3L8_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFT4084991.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            771..965
                     /gene="bfd"
                     /locus_tag="ACH3L8_001853"
     CDS             771..965
                     /gene="bfd"
                     /locus_tag="ACH3L8_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFT4084992.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1038..1514
                     /gene="bfr"
                     /locus_tag="ACH3L8_001854"
     CDS             1038..1514
                     /gene="bfr"
                     /locus_tag="ACH3L8_001854"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFT4084993.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1511..1978)
                     /locus_tag="ACH3L8_001855"
                     /pseudo
     CDS             complement(1511..1978)
                     /locus_tag="ACH3L8_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
     gene            2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACH3L8_001856"
     CDS             2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACH3L8_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFT4084994.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2701..3330
                     /gene="rplC"
                     /locus_tag="ACH3L8_001857"
     CDS             2701..3330
                     /gene="rplC"
                     /locus_tag="ACH3L8_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFT4084995.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3341..3946
                     /gene="rplD"
                     /locus_tag="ACH3L8_001858"
     CDS             3341..3946
                     /gene="rplD"
                     /locus_tag="ACH3L8_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFT4084996.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3943..4245
                     /gene="rplW"
                     /locus_tag="ACH3L8_001859"
     CDS             3943..4245
                     /gene="rplW"
                     /locus_tag="ACH3L8_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFT4084997.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4263..5084
                     /gene="rplB"
                     /locus_tag="ACH3L8_001860"
     CDS             4263..5084
                     /gene="rplB"
                     /locus_tag="ACH3L8_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFT4084998.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5101..5379
                     /gene="rpsS"
                     /locus_tag="ACH3L8_001861"
     CDS             5101..5379
                     /gene="rpsS"
                     /locus_tag="ACH3L8_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFT4084999.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5394..5726
                     /gene="rplV"
                     /locus_tag="ACH3L8_001862"
     CDS             5394..5726
                     /gene="rplV"
                     /locus_tag="ACH3L8_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFT4085000.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5744..6445
                     /gene="rpsC"
                     /locus_tag="ACH3L8_001863"
     CDS             5744..6445
                     /gene="rpsC"
                     /locus_tag="ACH3L8_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFT4085001.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6458..6868
                     /gene="rplP"
                     /locus_tag="ACH3L8_001864"
     CDS             6458..6868
                     /gene="rplP"
                     /locus_tag="ACH3L8_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFT4085002.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6868..7059
                     /gene="rpmC"
                     /locus_tag="ACH3L8_001865"
     CDS             6868..7059
                     /gene="rpmC"
                     /locus_tag="ACH3L8_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFT4085003.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACH3L8_001866"
     CDS             7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACH3L8_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFT4085004.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7478..7849
                     /gene="rplN"
                     /locus_tag="ACH3L8_001867"
     CDS             7478..7849
                     /gene="rplN"
                     /locus_tag="ACH3L8_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFT4085005.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7860..8174
                     /gene="rplX"
                     /locus_tag="ACH3L8_001868"
     CDS             7860..8174
                     /gene="rplX"
                     /locus_tag="ACH3L8_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFT4085006.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8189..8728
                     /gene="rplE"
                     /locus_tag="ACH3L8_001869"
     CDS             8189..8728
                     /gene="rplE"
                     /locus_tag="ACH3L8_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFT4085007.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8743..9048
                     /gene="rpsN"
                     /locus_tag="ACH3L8_001870"
     CDS             8743..9048
                     /gene="rpsN"
                     /locus_tag="ACH3L8_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFT4085008.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9082..9474
                     /gene="rpsH"
                     /locus_tag="ACH3L8_001871"
     CDS             9082..9474
                     /gene="rpsH"
                     /locus_tag="ACH3L8_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFT4085009.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9487..10020
                     /gene="rplF"
                     /locus_tag="ACH3L8_001872"
     CDS             9487..10020
                     /gene="rplF"
                     /locus_tag="ACH3L8_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFT4085010.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10030..10383
                     /gene="rplR"
                     /locus_tag="ACH3L8_001873"
     CDS             10030..10383
                     /gene="rplR"
                     /locus_tag="ACH3L8_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFT4085011.1"
                     /translation="MDKKSARIRRATRARRKLQELGATCLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10398..10901
                     /gene="rpsE"
                     /locus_tag="ACH3L8_001874"
     CDS             10398..10901
                     /gene="rpsE"
                     /locus_tag="ACH3L8_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFT4085012.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10905..11084
                     /gene="rpmD"
                     /locus_tag="ACH3L8_001875"
     CDS             10905..11084
                     /gene="rpmD"
                     /locus_tag="ACH3L8_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFT4085013.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11088..11522
                     /gene="rplO"
                     /locus_tag="ACH3L8_001876"
     CDS             11088..11522
                     /gene="rplO"
                     /locus_tag="ACH3L8_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFT4085014.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11530..12861
                     /gene="secY"
                     /locus_tag="ACH3L8_001877"
     CDS             11530..12861
                     /gene="secY"
                     /locus_tag="ACH3L8_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFT4085015.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACH3L8_001878"
     CDS             12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACH3L8_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFT4085016.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13156..13512
                     /gene="rpsM"
                     /locus_tag="ACH3L8_001879"
     CDS             13156..13512
                     /gene="rpsM"
                     /locus_tag="ACH3L8_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFT4085017.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13529..13918
                     /gene="rpsK"
                     /locus_tag="ACH3L8_001880"
     CDS             13529..13918
                     /gene="rpsK"
                     /locus_tag="ACH3L8_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFT4085018.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13952..14572
                     /gene="rpsD"
                     /locus_tag="ACH3L8_001881"
     CDS             13952..14572
                     /gene="rpsD"
                     /locus_tag="ACH3L8_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFT4085019.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14598..15587
                     /gene="rpoA"
                     /locus_tag="ACH3L8_001882"
     CDS             14598..15587
                     /gene="rpoA"
                     /locus_tag="ACH3L8_001882"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFT4085020.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15628..16011
                     /gene="rplQ"
                     /locus_tag="ACH3L8_001883"
     CDS             15628..16011
                     /gene="rplQ"
                     /locus_tag="ACH3L8_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFT4085021.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4141 a         3783 c         4292 g         3880 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggccgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgggctaatt acacgttaat
      601 tagttttgaa ttgaaaaggg cgcttcggcg ccctttttgc tgcccgtaat ttgagctcga
      661 aatatttagt cgtaattttg ctcgctgaac tatggcgcaa ataaaactgt gctattgtaa
      721 tcataaccat tctcatttac actttgtgca gaatttgaac gggagcaatc atgtacgttt
      781 gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag tttcatccgc
      841 aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt aagtgcattc
      901 gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat gccagaattt aaagagattg
      961 cctgaagccc acgcttattt tttgacatcc tgctagcccc atctacgctc taatagtgga
     1021 agcggaggga ctataaaatg aaaggtgatg ttaaaatcat aaattatctc aataaactat
     1081 tgggaaatga gcttgtcgca atcaatcagt attttctcca tgcccggatg tttaaaaact
     1141 ggggcctgat gcgtctcaat gacgtcgaat accatgaatc catcgatgag atgaaacacg
     1201 ctgataaata tatcgagcgt attttgtttt tagaaggaat ccccaactta caggatttag
     1261 gcaaactggg gattggtgaa gatgtcgagg agatgctgca atctgacctg cgattagaac
     1321 tggaaggcgc gaaggatctg cgtgaggcta tcgcatatgc cgacagcgtt catgactatg
     1381 ttagccgcga tatgatgatc gagatccttg ctgatgaaga aggtcatatc gactggctcg
     1441 aaactgaact ggatctcatc ggtaaaatcg gcctacaaaa ttacctgcaa tcacaaatta
     1501 aagttaaaga ttaacggcct atcagcaaac tatcccagcc tataacgaaa cccgcagccg
     1561 ccagaaatgg cccaaaaggc agcgggtttt ttaatgattc ttttcctctc atgagcagac
     1621 caataactac ggctccgcaa gcgaatgagg cagcaaggaa aaccagtcgt ggtaagaagg
     1681 cccatgaatg ctaggcgcca agggcggcga gaaacttcac atcaccatag cctaatcctt
     1741 ctttatgtcg cagtatgcga tacccccagt aaatgacagc gaatgtgccg taaccaacaa
     1801 ttgcccccca taacgcatcg gctaaaccat caggatgaca aacttgatag aacaaaagac
     1861 ctgaccagag taacgggcac gtaaatctgt caggtaacaa gccatgcttt gcatcccaga
     1921 aaaaaagtag ggctgataaa caagcgtaca aaattagaaa gggaagggta gtgtccataa
     1981 aacatcctct gtaattggag tatcaccatc gaataactcc ggatgctcag caacagagag
     2041 agcgctgatt tgttaacctg attccggtga aaaacgctat cttcacaacc ttacaaattt
     2101 cgtggaatgc agctcgcaat ctgcgcgttg cgattaattt ctacccgatg cttgcgtcag
     2161 gctcagattt gcgtataatg cgcgggcttg tcgtagttga cagcaggttc aatctgaacc
     2221 tcagttagcc gaatttggct acctaacaat gctcccaatc ggggagctac gtaagaacgg
     2281 ttacactctc ccatcaatcg taatgggtct gaggagtaat cattttcgtt tataaaataa
     2341 ttggagctct ggtctcatgc agaaccaaag aatccgtatc cgcctgaaag cgtttgatca
     2401 tcgtctgatc gatcaagcaa ccgcggaaat cgtcgagact gccaagcgca ctggtgcgca
     2461 ggtccgtggt ccgatcccgc tgccgacacg caaagagcgc ttcactgttc tgatctcccc
     2521 gcacgtcaac aaagacgcgc gcgatcagta cgaaatccgt actcacttgc gtctggttga
     2581 catcgttgag ccaaccgaga aaaccgttga tgctctgatg cgtctggatc tggctgccgg
     2641 tgtagacgtg cagatcagcc tgggttaatc aggtcattga gcgattgaga ggttgaaaca
     2701 atgattggtt tagtcggtaa aaaagtgggt atgacccgta tcttcacaga agacggcgtt
     2761 tctatcccag taaccgtaat cgaagttgaa gcaaaccgcg ttactcaggt taaagacctg
     2821 gctaacgatg gctaccgtgc tattcaggtg accaccggtg ctaaaaaagc taaccgtgtg
     2881 accaagcctg aagctggcca cttcgctaaa gctggcgtag aagctggccg tggtctgtgg
     2941 gaattccgcc tggctgaagg cgaagagttc actgtaggtc agagcattag cgttgaactg
     3001 tttgctgacg ttaaaaaagt tgacgtaact ggcacctcta aaggtaaagg tttcgcaggt
     3061 accgttaagc gctggaactt ccgtacccag gacgctactc acggtaactc cttgtctcac
     3121 cgcgttccgg gttctatcgg tcagaaccag actccgggca aagtgttcaa aggcaagaaa
     3181 atggcaggtc agatgggtaa cgaacgtgta accgttcaga gccttgacgt agtacgcgtt
     3241 gacgctgagc gcaacctgct gctggttaaa ggtgctgtcc cgggtgcaac cggtagcgac
     3301 ctgatcgtta aaccagctgt gaaggcgtaa ggagatagca atggaattag tattgaaaga
     3361 cgcgcagagc gcgctgactg tttccgaaac taccttcggt cgtgatttca acgaagcgct
     3421 ggttcaccag gttgttgttg cttatgcagc tggtgctcgt cagggtactc gtgctcagaa
     3481 gactcgtgct gaagtaactg gttccggtaa aaaaccgtgg cgccagaaag gcaccggccg
     3541 tgcgcgttct ggttctatca agagcccgat ctggcgttct ggtggcgtga cctttgctgc
     3601 tcgtccgcag gaccacagtc aaaaagttaa caagaagatg taccgcggcg cgctgaaaag
     3661 catcctgtcc gaactggtac gtcaggatcg tctgatcgtt gtcgagaagt tctctgtaga
     3721 agcgccgaaa actaagctgc tggcacagaa actgaaagac atggctctgg aagatgtgct
     3781 gatcatcacc ggtgagctgg acgaaaacct gttcctggct gcgcgcaacc tgcagaaggt
     3841 tgacgtacgc gatgcaactg gtatcgaccc ggttagcctg atcgccttcg acaaagtcgt
     3901 aatgactgct gatgctgtta agcaagttga ggagatgctg gcatgattcg tgaagaacgt
     3961 ctgctgaagg tgctgcgtgc accgcacgtt tctgaaaaag cgtctactgc gatggaaaaa
     4021 tccaacacca tcgtactcaa agttgctaaa gacgcgacca aagcagaaat caaagctgct
     4081 gtgcagaaac tgtttgaagt cgaagtcgaa gtcgttaaca ccctggtagt taaagggaaa
     4141 gttaaacgtc acggacagcg tatcggtcgt cgtagcgact ggaaaaaagc ttacgtcacc
     4201 ctgaaagaag gccagaatct ggacttcgtt ggcggcgctg agtaagtcgg aggagtaata
     4261 caatggcagt tgttaaatgt aaaccgacat ctccgggtcg tcgccacgta gttaaagtgg
     4321 ttaaccctga gctgcacaag ggcaaacctt ttgctccgtt gctggaaaaa aacagcaaat
     4381 ccggtggtcg taacaacaat ggccgtatca ccactcgtca tatcggtggt ggccacaagc
     4441 aggcttaccg tattgttgac ttcaaacgca acaaagacgg tatcccggca gttgttgaac
     4501 gtcttgagta cgatccgaac cgttccgcga acatcgcgct ggttctgtac aaagacggtg
     4561 aacgccgtta catcctggcc cctaaaggcc tgaaagctgg cgaccagatt cagtctggcg
     4621 ttgatgctgc aatcaaacca ggtaacaccc tgccgatgcg caacatcccg gttggttcta
     4681 ctgttcataa cgtagaaatg aaaccaggta aaggcggtca gctggcacgt tccgctggta
     4741 cttacgttca gatcgttgct cgtgatggtg cttatgtcac cctgcgtctg cgttctggtg
     4801 aaatgcgtaa agtagaagca gactgccgtg caactctggg cgaagttggc aatgctgagc
     4861 atatgctgcg cgttctgggt aaagcaggtg ctgcacgctg gcgtggtgtt cgtccgaccg
     4921 ttcgcggtac cgcgatgaac ccggtagacc acccacatgg tggtggtgaa ggtcgtaact
     4981 ttggtaagca cccggtaact ccgtggggcg ttcagaccaa aggtaagaag acccgcagca
     5041 acaagcgtac tgataaattc atcgtacgtc gccgtagcaa ataattttag aggataagcc
     5101 atgccacgtt ctctcaagaa aggtcctttt attgacctgc acttgctgaa gaaggtagag
     5161 aaagcggtgg aaagcggaga caagaagccc ctgcgcactt ggtcccgtcg ttcaacgatc
     5221 tttcctaaca tgatcggttt gaccatcgct gtccataatg gtcgtcagca cgttccggta
     5281 tttgtaaccg acgaaatggt cggtcacaaa ctgggtgaat tcgcaccgac tcgtacttat
     5341 cgcggccacg ctgctgataa aaaagcgaag aagaaataag gtaggaggaa gagatggaaa
     5401 ctattgctaa acatcgccat gctcgttctt ctgctcagaa ggttcgcctt gttgctgacc
     5461 tgattcgcgg taagaaagtg tcgcaggctc tggatatttt gacctacacc aacaagaaag
     5521 cggctgtaca ggtcaagaaa gttctggaat ctgccattgc taacgctgaa cacaacgatg
     5581 gcgctgacat tgacgatctg aaagttacga aaattttcgt agacgaaggc ccgagcatga
     5641 agcgcattat gccgcgtgca aaaggtcgtg cagatcgcat cctgaagcgc accagccaca
     5701 tcactgtggt tgtgtccgat cgctgagact ctggagacta gcaatgggtc agaaagtaca
     5761 tcctaatggt attcgcttgg gtattgtaaa accatggaac tctacttggt ttgcgaacac
     5821 caaagaattc gctgacaacc tggacagcga ttttaaagta cgtcagtacc tgactaagga
     5881 actggctaaa gcgtccgtat ctcgtatcgt tatcgagcgt ccggctaaga gcatccgtgt
     5941 aaccattcac actgctcgcc cgggtatcgt tatcggtaaa aaaggtgaag acgtagaaaa
     6001 actgcgtaag gtcgtagcgg acatcgctgg cgttcctgca cagatcaaca tcgccgaagt
     6061 tcgtaagcct gaactggacg caaaactggt tgctgacagc atcacttctc agctggaacg
     6121 tcgcgttatg ttccgtcgtg ctatgaagcg tgctgtacag aacgcaatgc gtctgggcgc
     6181 taaaggtatt aaagttgaag ttagcggccg tctgggcggc gcggaaatcg cacgtaccga
     6241 atggtaccgc gaaggtcgcg taccgctgca cactctgcgt gctgacatcg actacaacac
     6301 ctctgaagcg cacaccactt acggtgtaat cggcgttaaa gtgtggatct tcaaaggcga
     6361 gatcctgggt ggtatggctg ctgttgaaca accggaaaaa ccggctgctc agcctaaaaa
     6421 gcagcagcgt aaaggccgta aataaggagc gtcgctgatg ttacaaccaa agcgtacaaa
     6481 attccgtaaa atgcacaaag gccgtaaccg cggtctggcg cagggtacgg atgttagctt
     6541 cggcagcttc ggtctgaaag ctgttggccg tggtcgtctg actgcccgtc agatcgaagc
     6601 agcacgtcgt gctatgaccc gtgcagttaa gcgtcaaggt aagatctgga tccgtgtgtt
     6661 cccggacaaa ccgatcactg aaaagccgct ggcagtgcgt atgggtaaag gtaaaggtaa
     6721 cgtggagtat tgggttgcct tgattcagcc gggtaaagtc ctgtatgaaa tggacggtgt
     6781 tccggaagag ctggcccgtg aagcattcaa gctggcagca gcgaaactgc cgattaaaac
     6841 cacctttgta actaagacgg tgatgtaatg aaagcaaaag agctgcgtga gaagagcatt
     6901 gaagagctga acaccgagct gctgaacctg ctgcgtgagc agttcaacct gcgtatgcag
     6961 gctgcaagtg gccagctgca acagtctcac ctgttgaagc aagtgcgtcg cgatgtcgca
     7021 cgcgttaaga ctttactgaa cgagaaggcg ggtgcgtaat gaccgataaa atccgtactc
     7081 tgcaaggtcg cgttgttagc gacaaaatgg agaaatccat tgttgttgct atcgaacgtt
     7141 ttgtgaaaca cccgatctac ggaaaattca tcaagcgtac gaccaaactg cacgtacatg
     7201 acgagaacaa cgaatgcggt atcggtgacg tggttgaaat ccgcgaatgc cgtccgctgt
     7261 ccaagactaa atcctggacg ctggttcgcg ttgtagagaa agcggttctg taatacagta
     7321 cactctctcg atacgaataa acggctcaga aatgagccgt ttattttttc tacccatatc
     7381 cttgaagcgg tgttataatg ccgcgccctc gatatgggga tttttaacga cctgattttc
     7441 gggtctcagt agtagttgac attagcggag cactaaaatg atccaagaac agactatgct
     7501 gaacgtcgcc gacaactccg gtgcacgtcg cgtaatgtgt atcaaggttc tgggtggctc
     7561 gcaccgtcgc tacgcaggcg taggcgacat catcaagatc accatcaaag aagcaattcc
     7621 gcgtggtaag gtcaaaaaag gtgatgtgct gaaggcggta gtggtgcgca ccaagaaggg
     7681 tgttcgtcgc ccggacggtt ctgtcattcg cttcgatggt aatgcttgtg ttcttctgaa
     7741 caacaacagc gagcagccta tcggtacgcg tatttttggg ccggtaactc gtgagcttcg
     7801 tagtgagaag ttcatgaaaa ttatctctct ggcaccagaa gtactctaag gagcgaatca
     7861 tggcagcgaa aatccgtcgt gatgacgaag ttatcgtgtt aaccggtaaa gataaaggta
     7921 aacgcggtaa agttaagaat gtcctgtctt ccggcaaggt cattattgaa ggtatcaacc
     7981 tggttaagaa acatcagaag ccggttccgg ccctgaacca accgggtggc atcgttgaaa
     8041 aagaagccgc tattcaggtt tccaacgtag caatcttcaa tgcggcaacc ggcaaggctg
     8101 accgtgtagg ctttagattc gaagacggca aaaaagtccg tttcttcaag tctaacagcg
     8161 aaactatcaa gtaatttgga gtagtacgat ggcgaaactg catgattact acaaagacga
     8221 agtagttaaa aaactcatga ctgagtttaa ctacaattct gtcatgcaag tccctcgggt
     8281 cgagaagatc accctgaaca tgggtgttgg tgaagcgatc gctgacaaaa aactgctgga
     8341 taacgcagca gcagacctgg cagcaatctc cggtcaaaaa ccgctgatca ccaaagcacg
     8401 caaatctgtt gcaggcttca aaatccgtca gggctatccg atcggctgta aagtaactct
     8461 gcgtggcgaa cgcatgtggg agttctttga gcgcctgatc actattgctg tacctcgtat
     8521 ccgtgacttc cgtggcctgt ccgctaagtc tttcgacggt cgtggtaact acagcatggg
     8581 tgtccgtgag cagatcatct tcccagaaat cgactacgat aaagtcgacc gcgttcgtgg
     8641 tttggatatt accattacca ctactgcgaa atctgacgaa gaaggccgcg ctctgctggc
     8701 tgcctttgac ttcccgttcc gcaagtaagg tagggttact aaatggctaa gcaatcaatg
     8761 aaagcacgcg aagtaaaacg cgtagcttta gctgataaat acttcgcgaa acgcgctgaa
     8821 ctgaaagcga tcatctctga tgtgaacgct tccgacgaag atcgttggaa cgctgttctc
     8881 aagctgcaga ctctgccgcg tgattccagc ccgtctcgtc agcgtaaccg ctgccgtcaa
     8941 acaggtcgtc cgcatggttt cctgcggaag ttcgggttga gccgtattaa ggtccgtgaa
     9001 gccgctatgc gcggtgaaat cccgggtctg aaaaaggcta gctggtaatt gtcaccaatt
     9061 gaatcacggg agtaaagaca gatgagcatg caagatccga tcgcggatat gctgacccgt
     9121 atccgtaacg gtcaggccgc gaacaaagct gcggtcacca tgccttcctc caagctgaaa
     9181 gtggcaatcg ccaacgtgct gaaggaagaa ggttttattg aagattttaa agttgaaggc
     9241 gacaccaagc ctgaactgga acttactctg aagtatttcc agggcaaagc tgttgtagaa
     9301 agcattcagc gtgtcagccg cccaggtctg cgcatctata aacgtaaaga tgagctgccg
     9361 aaagttatgg cgggtctggg tatcgcagtt gtttctacct ctaaaggtgt tatgactgat
     9421 cgtgcagcgc gccaggctgg tcttggtggc gaaattatct gctacgtagc ctaatcggag
     9481 gaaaaaatgt ctcgtgttgc taaagcaccg gtcgttgttc ctgccggcgt tgacgtaaaa
     9541 atcaacggtc aggttattac gatcaaaggt aaaaacggcg agctgactcg tactctcaac
     9601 gatgctgttg aagttaaaca tgcagataat accctgacct tcggtccgcg tgatggttac
     9661 gcagacggtt gggcacaggc tggtaccgcg cgtgccctgc tgaactcaat ggttatcggt
     9721 gttaccgaag gcttcactaa gaagctgcag ctggttggtg taggttaccg tgcagcggtt
     9781 aaaggcaatg tgattaacct gtctctgggt ttctctcatc ctgttgacca tcagctgcct
     9841 gcgggtatca ctgctgaatg tccgactcag actgaaatcg tgctgaaagg cgctgataag
     9901 caggtgatcg gccaggttgc agcggatctg cgcgcctacc gtcgtcctga gccttacaaa
     9961 ggcaaggggg ttcgttacgc cgacgaagtc gtgcgtacca aagaggctaa gaagaagtaa
    10021 ggtaacacta tggataagaa atctgctcgt atccgtcgtg cgacccgcgc acgccgcaag
    10081 ctccaggagc tgggcgcaac ttgcctggtg gtacatcgta ccccgcgtca catttacgca
    10141 caggtaattg caccgaacgg ttctgaagtt ctggtagctg cttctactgt agaaaaagct
    10201 atcgctgaac aactgaagta caccggtaac aaagacgcgg ctgcagctgt gggtaaagct
    10261 gtcgctgaac gcgctctgga aaaaggcatc aaagatgtat cctttgaccg ttccgggttc
    10321 caatatcatg gtcgtgtcca ggcactggca gatgctgccc gtgaagctgg ccttcagttc
    10381 taaggtagag gtgtaagatg gctcacatcg aaaaacaagc tggcgaactg caggaaaagc
    10441 tgatcgcggt aaaccgcgta tctaaaaccg ttaaaggtgg tcgtattttc tccttcacag
    10501 ctctgactgt agttggcgat ggtaacggtc gcgttggttt tggttacggt aaagcgcgtg
    10561 aagttccagc agcgatccag aaagcgatgg aaaaagcccg tcgcaatatg attaacgtcg
    10621 cgctgaataa cggcactcta caacaccctg ttaaaggtgt tcacacgggt tctcgcgtat
    10681 tcatgcagcc ggcttccgaa ggtaccggta tcatcgccgg tggtgcaatg cgcgccgttc
    10741 tggaagtcgc tggggttcat aacgttctgg ctaaagcata tggttccacc aacccgatca
    10801 acgtggttcg tgcaactatt gatggcctgg aaaatatgaa ttctccagaa atggtcgctg
    10861 ccaagcgtgg taaatccgtt gaagaaattc tggggaaata aaccatggca aagactatta
    10921 aaattactca aacccgcagt gcaatcggtc gtctgccgaa acacaaggca acgctgcttg
    10981 gcttgggtct gcgtcgtatt ggtcacaccg tagagcgcga ggatactcct gctattcgcg
    11041 gtatgatcaa cgcggtttcc ttcatggtta aagttgagga gtaagagatg cgtttaaata
    11101 ctctgtctcc ggccgaaggc tccaaaaagg cgggtaaacg cctgggtcgt ggtatcggtt
    11161 ctggcctcgg taaaaccggt ggtcgtggtc acaaaggtca gaagtctcgt tctggcggtg
    11221 gcgtacgtcg cggtttcgag ggtggtcaga tgcctctgta ccgtcgtctg ccgaaattcg
    11281 gcttcacttc tcgtaaagca gcgattacag ccgaagttcg tctatctgac ctggctaaag
    11341 tagaaggcgg tgttgtagac ctgaacacgc tgaaagcggc taacattatc ggtatccaga
    11401 tcgagttcgc gaaagtgatc ctggctggcg aagtaacgac tccggtaact gttcgtggcc
    11461 tgcgtgttac taaaggcgct cgtgctgcta tcgaagctgc tggcggtaaa atcgaggaat
    11521 aagtagcaga tggctaaaca accgggatta gattttcaaa gtgccaaagg tggcttaggc
    11581 gagctgaaac gcagactgct gtttgttatc ggtgcgctga ttgtgttccg tattggctct
    11641 tttattccga tccctggtat tgatgccgct gtacttgcca aactgcttga gcaacagcga
    11701 ggcaccatca ttgagatgtt taacatgttc tctggtggtg ctctcagccg tgcttctatc
    11761 tttgctctgg ggatcatgcc gtatatttcg gcgtcgatca ttatccagct gctgacggtg
    11821 gttcacccaa cgttggcaga aattaagaaa gaaggggagt ctggtcgtcg taagatcagc
    11881 cagtacaccc gttacggtac tctggtgctg gcaatattcc agtcgatcgg tattgctacc
    11941 ggtctgccga atatgcctgg tatgcaaggc ctggtgatta acccgggctt tgcattctac
    12001 ttcaccgctg ttgtaagtct ggtcacagga acgatgttcc tgatgtggtt gggcgaacag
    12061 atcactgaac gaggtatcgg caacggtatt tcaatcatta tcttcgccgg tattgtcgcg
    12121 ggactcccgc cagccattgc ccatactatc gagcaagcgc gtcaaggcga cctgcacttc
    12181 ctcgtgttgc tgttggttgc agtattagta tttgcagtga cgttctttgt tgtgtttgtt
    12241 gagcgtggtc aacgccgcat tgtggtaaac tacgcgaaac gtcagcaagg tcgtcgtgtc
    12301 tatgctgcac agagcacaca tttaccgctg aaagtgaata tggcgggggt aatcccggca
    12361 atcttcgctt ccagtattat tctgttcccg gcgaccatcg cgtcatggtt cgggggcggt
    12421 actggttgga actggctgac aacaatttcg ctgtatttgc agcctgggca accgctttat
    12481 gtgttactct atgcgtctgc aatcatcttc ttctgtttct tctacacggc gttggttttc
    12541 aacccgcgtg aaacagcaga taacctgaag aagtccggtg catttgtacc aggaattcgt
    12601 ccgggagagc aaacggcgaa gtatatcgat aaagtaatga cccgcctgac cttggttggt
    12661 gcgctgtata ttacctttat ctgcctgatc ccggagttca tgcgtgatgc aatgaaagta
    12721 ccgttctact tcggtgggac ctcactgctt atcgttgttg tcgtgattat ggactttatg
    12781 gctcaagtgc aaactctgat gatgtccagt cagtatgagt ctgcattgaa gaaggcgaac
    12841 ctgaaaggct acggccgata attggtcgcc cgagaagtta cggagagtaa aaatgaaagt
    12901 tcgtgcttcc gtcaagaaat tatgccgtaa ctgcaaaatc gttaagcgtg atggtgtcat
    12961 ccgtgtgatt tgcagtgccg agccgaagca taaacagcgc caaggctgat tttttcgcat
    13021 atttttcttg caaagttggg ttgagctggc tagattagcc agccaatctt ttgtatgtct
    13081 gtacgtttcc atttgagtat cctgaaaacg ggcttttcag catggaacgt acatattaaa
    13141 tagtaggagt gcatagtggc ccgtatagca ggcattaaca ttcctgatca taagcatgcc
    13201 gtaatcgcat taacttcgat ttatggcgtc ggcaagaccc gttctaaagc catcctggct
    13261 gcagcgggta tcgctgaaga tgttaagatc agtgagctgt ctgaaggaca aatcgacacg
    13321 ctgcgtgacg aagttgccaa atttgtcgtt gaaggtgatc tgcgccgtga aatcagcatg
    13381 agcatcaagc gcctgatgga tcttggttgc tatcgcggtt tgcgtcatcg tcgtggtctc
    13441 ccggttcgcg gtcagcgtac caagaccaac gcacgtaccc gtaagggtcc gcgcaaaccg
    13501 atcaagaaat aatcggggtg attgaataat ggcaaaggca ccaattcgtg cacgtaaacg
    13561 tgtaagaaaa caagtctctg acggcgtggc tcatatccat gcttctttca acaacaccat
    13621 cgtgactatc actgatcgtc agggtaacgc gttgggttgg gcaacagccg gtggttccgg
    13681 tttccgtggt tctcgcaaat ccactccgtt tgcagctcag gttgcagcag agcgttgcgc
    13741 tgacgccgtg aaagaatacg gcatcaagaa tctggaagtt atggttaaag gtccgggtcc
    13801 aggccgcgaa tctactattc gtgctctgaa cgccgcaggt ttccgcatca ctaacattac
    13861 tgatgtgact ccgatccctc ataacggttg tcgtccgccg aaaaaacgtc gcgtataacg
    13921 cctcgttttc caggtttgtt ggagaaagaa aatggcaaga tatttgggtc ctaagctcaa
    13981 gctgagccgt cgtgagggca ccgacttatt ccttaagtct ggcgttcgcg cgatcgatac
    14041 caagtgtaaa attgaacaag ctcctggcca gcacggtgcg cgtaaaccgc gtctgtctga
    14101 ctatggtgtg cagttgcgtg aaaagcaaaa agttcgccgt atctatggtg tgctggagcg
    14161 tcagttccgt aactactaca aagaagcagc acgtctgaaa ggcaacaccg gtgaaaacct
    14221 gttggctctg ctggaaggtc gtctggacaa cgttgtatac cgtatgggct tcggtgccac
    14281 tcgtgcagaa gcacgtcagc tggttagcca taaagcaatt atggtaaacg gtcgtgttgt
    14341 taacatcgct tcttatcagg ttagtccgaa tgacgttgta agcattcgtg agaaagcgaa
    14401 gaagcagtct cgcgtgaaag ccgctctgga gctggctgag cagcgtgaaa agccaacttg
    14461 gctggaagtt gatgctggca agatggaagg tacgtttaag cgtaagccgg agcgttctga
    14521 tctgtctgcg gacattaacg aacacctgat cgtcgagctt tactccaagt aaagcttagt
    14581 accaaagaga ggacacaatg cagggttctg tgacagagtt tctaaaaccg cgcctggttg
    14641 atatcgagca agtgagttcg acgcacgcca aggtgaccct tgagccttta gagcgtggct
    14701 ttggccatac tctgggtaac gcactgcgcc gtattctgct ctcatcgatg ccgggttgcg
    14761 cggtgaccga ggttgagatt gatggtgtac tacatgagta cagcaccaaa gaaggcgttc
    14821 aggaagatat cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga gttcagggca
    14881 aagatgaagt tattcttacc ttgaataaat ctggcattgg ccctgtgact gcagccgata
    14941 tcacccacga cggtgatgtt gaaatcgtca agccgcagca cgtgatctgc cacctgaccg
    15001 atgagaacgc gtctattagc atgcgtatca aagttcagcg cggtcgtggt tatgtgccgg
    15061 cttctacccg aattcattcg gaagaagatg agcgcccaat cggccgtctg ctggtcgacg
    15121 catgctacag ccctgtggag cgtattgcct acaatgttga agcagcgcgt gtagaacagc
    15181 gtaccgacct ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc gatcctgaag
    15241 aggcgattcg tcgtgcggca accattctgg ctgaacaact ggaagctttc gttgacttac
    15301 gtgatgtacg tcagcctgaa gtgaaagaag agaaaccaga gttcgatccg atcctgctgc
    15361 gccctgttga cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa gcagaagcta
    15421 tccactatat cggtgatctg gtacagcgta ccgaggttga gctccttaaa acgcctaacc
    15481 ttggtaaaaa atctcttact gagattaaag acgtgctggc ttcccgtgga ctgtctctgg
    15541 gcatgcgctt ggaaaactgg ccaccggcaa gcatcgctga cgagtaaccg gatcacaggt
    15601 taaggtttta ctgagaagga taaggtcatg cgccatcgta agagtggtcg tcaactgaac
    15661 cgcaacagca gccatcgcca ggctatgttc cgcaatatgg caggttcact ggttcgtcat
    15721 gaaatcatca agacgactct gcctaaagcg aaagagctgc gccgcgtagt tgagccgctg
    15781 attactcttg ccaagactga tagcgttgct aatcgtcgtc tggcattcgc ccgtactcgt
    15841 gataacgaga tcgtggcaaa actgtttaac gaactgggcc cgcgtttcgc gagccgtgcc
    15901 ggtggttaca ctcgtattct gaagtgtggc ttccgtgcag gcgacaacgc gccgatggct
    15961 tacatcgagc tggttgatcg ttcagagaaa gcagaagctg ctgcagagta atctgaagca
    16021 atgtaaaaaa acccgccccg gcgggttttt ttatacccgt agtatcccca cttatctaca
    16081 atagctgtac tctttt
//
LOCUS       DAZXUK010000075        16030 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZXUK010000075 DAZXUK010000000
VERSION     DAZXUK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16030
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.075"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..412)
                     /locus_tag="ACH3L8_001884"
     CDS             complement(<1..412)
                     /locus_tag="ACH3L8_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HFT4085022.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKK"
     gene            512..2203
                     /locus_tag="ACH3L8_001885"
                     /pseudo
     CDS             512..2203
                     /locus_tag="ACH3L8_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            2268..2963
                     /gene="queC"
                     /locus_tag="ACH3L8_001886"
     CDS             2268..2963
                     /gene="queC"
                     /locus_tag="ACH3L8_001886"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFT4085023.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(3015..3413)
                     /gene="fadM"
                     /locus_tag="ACH3L8_001887"
     CDS             complement(3015..3413)
                     /gene="fadM"
                     /locus_tag="ACH3L8_001887"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFT4085024.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(3507..3878)
                     /locus_tag="ACH3L8_001888"
     CDS             complement(3507..3878)
                     /locus_tag="ACH3L8_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFT4085025.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(4029..5900)
                     /gene="ppiD"
                     /locus_tag="ACH3L8_001889"
     CDS             complement(4029..5900)
                     /gene="ppiD"
                     /locus_tag="ACH3L8_001889"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFT4085026.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(6092..6382)
                     /gene="hupB"
                     /locus_tag="ACH3L8_001890"
     CDS             complement(6092..6382)
                     /gene="hupB"
                     /locus_tag="ACH3L8_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFT4085027.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(6573..8927)
                     /gene="lon"
                     /locus_tag="ACH3L8_001891"
     CDS             complement(6573..8927)
                     /gene="lon"
                     /locus_tag="ACH3L8_001891"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFT4085028.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(9115..10389)
                     /gene="clpX"
                     /locus_tag="ACH3L8_001892"
     CDS             complement(9115..10389)
                     /gene="clpX"
                     /locus_tag="ACH3L8_001892"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFT4085029.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(10515..11138)
                     /gene="clpP"
                     /locus_tag="ACH3L8_001893"
     CDS             complement(10515..11138)
                     /gene="clpP"
                     /locus_tag="ACH3L8_001893"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFT4085030.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(11385..12683)
                     /gene="tig"
                     /locus_tag="ACH3L8_001894"
     CDS             complement(11385..12683)
                     /gene="tig"
                     /locus_tag="ACH3L8_001894"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFT4085031.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(13027..13344)
                     /gene="bolA"
                     /locus_tag="ACH3L8_001895"
     CDS             complement(13027..13344)
                     /gene="bolA"
                     /locus_tag="ACH3L8_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFT4085032.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            13649..14227
                     /locus_tag="ACH3L8_001896"
     CDS             13649..14227
                     /locus_tag="ACH3L8_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFT4085033.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            14271..15746
                     /gene="ampG"
                     /locus_tag="ACH3L8_001897"
     CDS             14271..15746
                     /gene="ampG"
                     /locus_tag="ACH3L8_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFT4085034.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         3648 a         4320 c         3815 g         4247 t
ORIGIN      
        1 tttttttgac atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg
       61 cagggctctc tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg
      121 tatcccattg ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta
      181 aaagttcacc ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca
      241 gcgatagcgc cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga
      301 gggtaatgtc gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat
      361 ggtcaggcat caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct
      421 ccactctgtg acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt
      481 gaatagttaa ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta
      541 tcaacgtctg tggcaacctt cagccgcaaa ccgtcaccgt cagcgaactg gccgaacgct
      601 gtttttgcag cgaacgccat gttcgtacgc tgttgcgtca ggcacaggag gcgggatggc
      661 tggagtggca ggcgcagtca ggacgcggaa agcgcggaca attacgcttt ctggtcacgc
      721 cggaatcgct acgcaatgcg atgatggaac aggcactgga aaccggaaag cagcaagatg
      781 tgctggagct ggcgcaactg gccccaggtg agctgcgcac tctgttacag ccatttatgg
      841 gcggacaatg gcaaaacgat acacccacgt tgcgtattcc ctactatcgc ccgctcgaac
      901 cgctacaacc aggctttttg cccggccgtg ccgagcagca tctcgccggg cagatatttt
      961 ccggcctgac ccgcttcgat aataatactc agcgcccgat tggcgattta gcgcatcact
     1021 gggaaacctc tactgacagg ttacgctggg acttttatct tcgttcaacc ctacactggc
     1081 ataacggcga tgcagtaaaa gcctcacact tacaccagcg attattgatg ctgttacaac
     1141 tgccagcact ggatcaatta tttattagcg tgaagcgtat tgaagtcacc catccgcagt
     1201 gtctgacctt ctttttacat cgccccgatt actggcttgc gcaccggctg gcgagctatt
     1261 gcagccatct ggcgcatccg caattcccac tgatcggcac tggtcctttt cgcttaacac
     1321 aattcacagc ggagctggtg cgcctggaga gccatgatta ttaccatttg cgtcatccgc
     1381 tgcttaaagc ggttgagtac tggataactc cgccgctttt cgaaaaagat ttgggaacca
     1441 gttgtcggca tcccgtgcaa atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc
     1501 aagtcagtag tggcatcagt ttaggttttt gctatttaac gttgcgcaaa agtccccgac
     1561 tctccctctg gcaggcgcga aaagtgatct ccattattca tcaatccggt ttattacaaa
     1621 cgttagaagt cggagaaaac ctgatcaccg ccagtcatgc attactgcca ggctggacta
     1681 ttccgcactg gcaagtaccg gatgaagtca aactaccgaa aaccttgacg ctggtttatc
     1741 acctaccgat agaacttcat accatggcag aacgcctaca ggcgacactg gcagcggaag
     1801 gctgtgaact cacaattatt tttcataacg caaaaactgg gacgacacga ccctactggc
     1861 acacgcagac ctcatgatgg gcgacagatt aattggcgaa gcaccggaat atactctgga
     1921 gcaatggcta cgttgcgatc cactatggcc acatgttttc gacgctccag catatgcaca
     1981 tctgcaatcg acgctggacg cggtgcaagt aatgcctgat gaggaaaacc gatttaatgc
     2041 cctgaaagcg gtttttagcc agttaatggc agacgcgacg ctgacgccgc tgttcaacta
     2101 tcactatcgc attagtgccc ctcccggcgt gaacggtgtg cgactgacac cgcgcggctg
     2161 gtttgaattt accgaagcct ggcttcccgc gccgtcgcaa tgaatagctg gtccgggtta
     2221 atcgcaggcg ttaccataac gcttttattg ttcaagcagg attatctatg aaacgtgctg
     2281 tcgttgtgtt cagtggaggc caggattcca ccacctgtct ggtgcaggca ttacaacaat
     2341 atgatgaagt ccattgcgtg acgttcgatt acggtcagcg acatcgcgca gaaatcgacg
     2401 tggcacgcga actggcgctg aaactgggag cacgcgcgca taaggttctt gatgtaacgc
     2461 tgctcaacga gctggcggtc agcagcctga cacgtgacag cattccggtg cctgattatg
     2521 aacctgaagc cgatggcatc ccgaatacgt ttgtcccagg gcgtaatatt ttgttcctga
     2581 cgctggcggc aatatatgcg tatcaggtaa aagcagaagc cgtaattacc ggcgtctgtg
     2641 aaacggattt ctccggctac ccggattgcc gcgatgagtt tgtgaaagca ctaaaccatg
     2701 ccgtcagttt gggcatggcg aaagatattc gttttgaaac gccgctgatg tggattgata
     2761 aagcggaaac ctgggcgctg gcagattatt acggcaaact ggatttagtc cgtaacgaaa
     2821 cgttgacctg ctataacggc attaaaggcg acggttgcgg tcattgtgcg gcatgtaatt
     2881 tacgcgccaa cggtttgaat cattatctgg ccgataaacc gacggtgatg gcagcgatga
     2941 agcagaaaac cgggttgagg taattattcc gggtgtcgcc ggatgcggct tgagcatccg
     3001 gcaccacaaa acgtttactt aaccatctgc tccagctttt cgcgcaattc cccttccaga
     3061 gctaatgctt tctgcgtttt aagatcaata caaacaaacg taataagcgc atccgctacc
     3121 acctgccctt ccggctccag tgtaatgacc tggtttaaga tgccgctttt accgtttaat
     3181 tgctgcaact gactggtaat agttaacagg tcgcttaata ccgctggacg acgatagtta
     3241 atattgatat tgaccacgac gaaggcgatg ttatgggccg tcatccactg aaaactgtcg
     3301 ctattttcca ccccatccca gcgggcttct tcgagaaatt caaggtagcg ggcgttgttg
     3361 acgtgctggt aaacgtcgag atgatatcca cgaactttga tttgtgtttg catagcgcaa
     3421 taaccttacg ttgttgttat aagtacagag gtcataactg gtatgacctc ttcagtctgg
     3481 caaaaaattg ccactatgca aattaattac agggttaata ccgccagatt acgttccacc
     3541 agcgaattgc ccatccccgg cacctgcttt agatcctcca ccgttttaaa cggaccgtac
     3601 tcttcgcgat aactgacaat cgcctgcgct ttcttcaggc caacgccatt catcgcgcgg
     3661 gctagctctt ccgcgctggc attattaatg ctgacccggg tgccttcttc gtcactggct
     3721 ttcgccggta ctgctgcttt actttgtgct gcaggagctt ccgctttggt ttctaccgcc
     3781 gtcggtttcg ccacagaagc tgccgccagc gcgctatgag acattccggc acaggccagg
     3841 gacagtgtaa tgagcagtgc tttaattccg tgtttcatac tgttttctcc ttgtttgtta
     3901 acagtggaat caccttaacg ggagtgaaaa cagtaaacaa atggcaatgt tcagaaatgg
     3961 aaaaggccgc gaaagcgacc ttttcttgtt acagctgcaa cgcatttttt tgcgaggcgg
     4021 cttcaggatt attgctgttc cagcgcatcg ccaattttga ttttcgcctc tttacgcagg
     4081 ttactcatca gagcttcaaa gacgatttgt gcgttgttct gggtgatacc ctgcaccatc
     4141 gcttttttct gatcttccgg cattgaacct tgtttcactt catccagcgc cagcagaacc
     4201 acattacctt gcatatcggt cgccataccg tagctcggtt tgtctttcgc tggcagtggc
     4261 agtgcaaacg ccgcctggct aatcgggtca cgaccggaac ggcttaaggt tttcggctcg
     4321 ccaaatttca gaccggcagc ctgcatagct tccgcacctt tgccggcttt caaatcaacc
     4381 agcagtttct cagcatccac tttcgcctgt tgttcagctt tgttgtgctg aaccagtgcc
     4441 ttaacttgtt cctgaacatc tgccaacggt ttcaccgctt ccggtttgtg ctcgctgacg
     4501 cgcagcacga atgcgcggtc gccgtctacg gtgatgatgt cagagttgat gcccgacgcg
     4561 ccgttttcac ctaccagacc gccgttaaag atagcgtcgg caaccggctt gaagttcaac
     4621 tcttccggca ggttatcttt gctgaaccaa cccgtctgag tggctttaac gccggcagct
     4681 tgctctgcac cggccagaga ctcggtgtcg ttgcttgccg catcgctcac tttctgctgc
     4741 agcgcatagt acgcatcgag ggctttttcg tgtttcactt tcgccgcaat gtcgtcacgt
     4801 acttcgtcta acgatttcac tttcgctggc tgaatgtcgt ccagacgtac aatcaggaaa
     4861 ccgaccgaag atttgatgac accagacagt tggccttttt ctttcagacc agcatttttc
     4921 agttcgtcgg ggatagtggc atcttctaac caacccatat cgccgccgtt acgggcagag
     4981 ataatatcgg cagatttttc tttggctaat gcagcaaaat caccgccttt attcagctca
     5041 tcaagtaccg ctttcgcttc atcttcagtt ttggtctgga tgatgctgta gcgggtacgc
     5101 tgcggctggg tgaattgatc ctgatgctga tcgtagtagc tctggatatc cgcatcgcta
     5161 accggttgct gcatcgttgc ggcaaccagc ttgatgtaac tcacgcggaa ttgttccggc
     5221 gtcatgaaat tgtttttgtt ttgttcgtag tagctggcaa tctcctgttc ggtcacaggc
     5281 tgcttcgccg ccagcgcgtt aacatcgata gtcgcctcac gcaccacgcg ttgttgcgcg
     5341 accagtgccg ccagctcgtc ggtttcacct ttcagcataa aatcggtacc ggcaacgccg
     5401 ttaatcagct gttgggtagt gagctggtta cgcagcgcct gggcgtactg atcggcggtc
     5461 atccccatct ggttgaggat accgttatag cggctgttat caaatttgcc gtcaacctgg
     5521 aaggctgggg tcgcgaaaat cgcctgttta acctgctcat cactgatacc cagtttcagc
     5581 tcacgagcgt actgatccaa cagcgcctcg tcgatcagac gattcagcac ctgttgacgc
     5641 agggttttca tatagccttc gttcgctgcc agctcggagt attgatcgcc cagctgttgc
     5701 tgcatacgat tacgctcgct gttaaaggcg ttctcgaact gcccacggct gatttcctgg
     5761 tcattcactt ttgcggcgta gttattgcct ccgccaatca ggtaaccact cacgccggtc
     5821 aatatgaacg acacgataat gataccgaaa ataatcttga gcacgagact gtttgcagcc
     5881 gtgcgtaagc tgtccatcat ggtgtaacaa cactccgctg tagatgactg tgtacctcac
     5941 gcaacgtatc gatgcgcgca aaggcctatt gtgacaagaa acgggggcaa ttgtcagccc
     6001 acaagacaca gaaagtcacc gaatacaaat aaaaaaggca catcagtaga tgcgcccttg
     6061 aacttcgtca catccccact ggggacaacg cttagtttac cgcgtctttc agtgctttac
     6121 ctgcacggaa gctcggtact ttagcggcag cgatggtgat ctctttaccg gtctgcgggt
     6181 tgcggccagt acgggcagca cgctctttaa cggcaaaagt accaaaacct accagtgcta
     6241 catcatcccc ttctttcaga gattcagtta cggaagcaat aatagcatct aacgcacggc
     6301 cagccgcagc tttagagata tcagcccctg cagcaatctt gtcgatcaat tgagatttat
     6361 tcactcttct cttcctcttt ataatttata tcgcacttga atccttcaag gtacgaacgc
     6421 gcagcagtta tatcaggcca gccatcccct tacaacaccc cttaataagg gcaagcccga
     6481 tccgctatct aacttagcga gacaaaaaaa ggctggcaag cccgaattag cctgccagcc
     6541 ctgtttttat tagtgcattt tgcgcgaggt cactattttg cagtcacaac ctgcatgcca
     6601 gacggttcat tttgcagcgc cagagtcaga acttcctcaa tgcgcttcac aggatgaatg
     6661 tccagatcgg caattacgtt gtcaggaatc tcttccagat cgcgtttatt ttcgaacgga
     6721 attagcactg ttttaatccc gccgcgatgc gctgccagga gtttttcttt caaaccaccg
     6781 atcggcagta cctgaccacg cagagtgatc tcaccggtca ttgccacatc ggcacgaacc
     6841 gggttaccgg tcaggcaaga aaccagcgcg gtgcacatag caataccggc actcggacca
     6901 tctttcggcg tcgcaccttc cggtacgtgg acgtggatgt cacgtttttc gtaaaaatca
     6961 gggttgatcc ccagtttttc cgcacgcgca cgaaccaccg ttaacgccgc ctgaatagac
     7021 tcctgcatca cttcgccgag cgaaccggta taggtcagtt tgcctttacc cggaacacat
     7081 gcggtttcaa tggtcagcaa gtcaccgccc acttccgtcc acgccagacc gattacctga
     7141 ccgacacggt tttcgttatc agcgcgacca tagtcgaaac gctgaacacc gaggtagtca
     7201 tgcaggttat cgccgttaat ttcgatatgt tttaatgact tatcgagcag taactgctta
     7261 accgctttgc ggcacagttt ggagatttca cgctccagac cacgcacgcc cgcctcacgg
     7321 gtgtagtaac gaataatgcc gataatggcg ctatcgtcga cggtcagctc accttttttc
     7381 agtgcattac gttcaatctg cttcggcagc aggtgacgtt tggcgatgtt cagtttttca
     7441 tcttcggtat aaccggagag gcgaatcact tccatacgat ccagcagcgg tgccggaatg
     7501 ttcatggagt tcgacgtcgc gacaaacatc acgtcgctga gatcgtaatc cacttccagg
     7561 tagtggtcgc tgaacgctac gttctgctct ggatccagca cttcaagcag tgcagaggcc
     7621 ggatcgcctc gcatgtcaga agacattttg tcgatctcat cgagcaggaa cagcggattt
     7681 ttcacgccca ctttcgccat tttctggatc agtttacccg gcatagaacc gatgtaagta
     7741 cggcggtgac cacggatttc cgcttcatca cgcacgccgc ccagcgccat acggacatat
     7801 ttacgcccgg tggctttggc aatggactga ccaagagagg ttttacctac ccccggcggc
     7861 cctaccaggc agaggatcgg tcccttgatt ttgttgacac ggctttgaac cgcaaggtac
     7921 tcaaggatgc gatctttcac gcgctccaga ccataatggt cggtatcaag gatttcctgc
     7981 gcctgacgca ggtctttttt gaccttgctg cgcgcattcc acggtacctg taccatccag
     8041 tcgatataac cacgcactac ggtcgcttct gccgacatcg gagacatcat tttcagcttc
     8101 tgcaactctg cttccgcttt ctcttttgcc tctttcggca tcttcgccgc gtcgattttg
     8161 cgcttcaggg cttcgttttc gtccggcacg tcgtccattt caccgagttc tttctgaata
     8221 gctttcattt gctcgttcag atagtactca cgctgggatt tctccatctg ctttttaacg
     8281 cggttgcgaa tgcgtttctc aacctgcagc agatcgattt ccgattccat cattgccatc
     8341 agatattcca gacgttcgtt aacgtcggac atctccagaa cggactgttt gtcagccagt
     8401 ttcagcggca tatgtgcagc aatggtatcc gccagacgcg ccggatcgtc gatgctattc
     8461 agcgacgtca gcacttctgg tgggattttt ttgttcagct tgatgtagcc ttcgaactgg
     8521 ctgattgcag tacgcaccag cacttcctgt tcccgctcat caatggtcgg cgactccaga
     8581 tactccgcct tcgcagaaaa gtgttcgcca ttgtcagaga gcgcagaaat acgcgcgcgc
     8641 tgtaacccct cgaccagcac tttgacggtg ccgtcaggca gtttcagcat ctgcaatata
     8701 gaggccacgg tcccgacggt gaaaagatcg tttacacccg gctcatccgt tgaagcttct
     8761 ttctgcgcga ccagcataat ttttttatca tggtccatcg ccgcttccag acaacggata
     8821 gatttttccc gcccgacaaa taaggggatg accatgtgcg gataaaccac cacatcgcgc
     8881 agcggcaata cggggatttc aatgcgttca gaacgctcag gattcataga gctctctctt
     8941 agtttaattt ccgccaggta atcagatgac acgactgtgc ttcacgccat ctattaacat
     9001 gtacgtcagt atatggggat gtttccccca cattcaacgc cgagaataga ggaaaaatta
     9061 aaggggagat aaaatccccc ctttttggtt aactaattgt atgggaatgg ttaattattc
     9121 accagatgcc tgttgcgctt ccggcttacc ataaatcagc aacggtttgc tttgaccatc
     9181 aattaccgac tcgtcgataa ccactttttc gacgtcttcc atggacggca gatcgtacat
     9241 ggtatcgagc agtgcggctt ctacgatgga acgcaggcca cgggcaccgg ttttacgcgc
     9301 catcgctttc ttagcgatag catccagcgc ctcgtcacgg aattccagat ccacgccttc
     9361 cagattaaac agcgcctgat actgcttggt cagggcgttt ttcggctctt tgaggatctg
     9421 aatcagagct tcttcgctca gttcattcaa cgttgctaca accggcagac gaccaatgaa
     9481 ctcagggata agaccaaact tgatcagatc ttccggttca acctgcgcca gcagctcgcc
     9541 ttcgcttgct ttgtcggact tcgcttttac cgtcgcgcca aaaccaatgc cggagccggt
     9601 ttctacacgg tgggaaatca ctttatccag accggcaaac gcaccgccac aaataaacag
     9661 gatcttagag gtatcaacct gcaagaattc ctgctgcgga tgtttacgcc caccttgcgg
     9721 tggaacagca gctaccgtac cttcgatcag tttcaacagt gcctgctgta ctccttcacc
     9781 ggaaacgtct cgggtaatgg acgggttgtc tgacttacga gaaatcttgt cgatttcatc
     9841 gatgtagaca atcccgcgct gcgctttctg tacgtcgtaa tcgcactttt gcaacagctt
     9901 ctgaatgatg ttttcaacgt cttcgcccac ataaccggct tcggtcagcg tggttgcgtc
     9961 ggccatggtg aacgggacgt ccagcaggcg cgccagcgtt tcagccagca gcgttttacc
    10021 ggaaccggtc ggaccgatca gcagaatgtt acttttgccc aactcgacac cattgctggt
    10081 atcgccgttg cgcagacgtt tgtaatggtt gtataccgcg accgccagca cttttttcgc
    10141 ctgttcctgg ccgataacgt aatcgtccag gtggttgcga atttcatgcg gcgtcggtag
    10201 cgcactgcgt tcacgatgcg gtgcaacttc tttaatctct tcgcgaatga tgtcgttaca
    10261 taaatcaaca cattcgtcgc agatatacac ggatggaccg gcaatcagct tgcgcacttc
    10321 atgctggctt ttgccgcaaa aagagcaata cagcaatttg cctgagccat ctttgcgttt
    10381 atctgtcatg ggtcaaaacc tcttctttgt tctttgtgcc gcacacgacg acgcaaatgc
    10441 cattctcagg cgcaagccgc ttacagcgtt gtgccgccct agataagtat agcggcacag
    10501 ttgcgcctct ggcatcaatt acgatgggtc agaatcgaat cgaccagacc gtattccacc
    10561 gcttcagggg cggaaaggaa gcgatcgcgc tcggtatcac gttcaatctg ttctaatgat
    10621 tgacccgtat gaagcgccat aagttcattc atgcgccctt taactttcag aatttcacgg
    10681 gcatgaattt cgatatcggt cgcctggccc tggtagccgc ccaacggttg gtgaatcatc
    10741 acgcgcgaat tcggcaggca aaaacgttta ccttttgccc ctgcggtcaa caagaaagcg
    10801 cccatcgagg ccgcctggcc catacagatg gtgctgacat caggcttgat aaactgcatg
    10861 gtgtcataga tagacatccc ggcagtgatc accccgcctg gggagttaat gtacagatag
    10921 atatcttttt ctgggttttc cgcttccagg aacagcatct gcgccacaat caggttagcc
    10981 atgtggtctt caacctggcc agtcagaaaa atgacgcgtt ccttaagtag acgagaatag
    11041 atatcaaaag agcgctcacc gcgtgaggtc tgttcaatga ccatcggcac cagcgccata
    11101 tggggtgcaa agttatctcg ttcgccgctg tatgacattt ccgtctcctg gataaaattg
    11161 aaaaaaacct gctgtaccga ttgtaacctt atggacggat tatcagctag tccctgtgtt
    11221 atgggtacgg catcaccata tttcaagcat aacaatcttt tgttgttacg ctaacaccga
    11281 aagaggcttt tcgcacggct ccatgcaaaa ttcatgacaa aaaaaagccc accacctggc
    11341 ggtgacgggc ctttgtgcga atttagcgcg ttatgctgcg taaattacgc ctgctggttc
    11401 atcagctcgt tgaaagtggt ttctttttca gtcactttcg ctttcgccag tacagcttca
    11461 acagcctgtt cttccagagc aacattgcgc atgttgtcca tcagttcttt gtttttgctg
    11521 tagaactcga taacttcttt cggatcttcg tacgcagaag ccatctcttc gatcaggcct
    11581 ttcacgcgct cttcgtcagc tttcagctcg ttggtgcgga taacttcgcc cagcagcagg
    11641 ccaacaacta cgcggcgttt agcctgttct tcgaacagtt cgcgcggcag ttccagagct
    11701 tgtttttcgt tgccaccgaa acgctgtgca gcctggcgac gcagaacgtc gatttcgctg
    11761 tcgatcagcg cagccggtac gtcgatgtcg ttagctttta ccagaccttc gatcgcctga
    11821 gacttaacgc ggttacggat ggcgctcttc agctcgcgct ccatgttttt acgcacttca
    11881 gcgcgcagac cttctacgga accatcttca acgccgaaac gtttgatgaa ttctgcagtc
    11941 agttccggca gttcacgctc ttcaactttc ttcaggttga tagcgaattt cgctgcttta
    12001 cctttcaggt tttctgcgtg gtattcttcc gggaaggtca cgtcgatggt gaactcttcg
    12061 ccagctttgt ggcctttgat accgtcttca aagcccggga tcatacgacc ctggcccatc
    12121 gccagtacga aatcagacgc tttaccgcct tcgaactctt cgccgtctac agaaccggtg
    12181 aagtcgatgg ttacgcggtc ttctgcttca acagcgccgt ctttttcttt ccaggtcgcc
    12241 tgctgtttac gcagagtatc cagcatgccg tcaacgtcag cgtcggtcac ttcaacgatc
    12301 ggtttttcaa cttcgatcgc ttccagaccc tgcagttcaa cttccggata aacttcaaac
    12361 tctacagagt aagtgaagtc ttcacccagc ttgtattcgc ccggaacata agtcggtgcg
    12421 ccagccggat tgattttttc tttaatgatg gcgtcaatga agttacggct catcaggtca
    12481 cccagaacgt cctggcgtac agacgcgcca taacgctgag caacgatatt cattggcact
    12541 ttgcctttgc ggaagccgtc aatacgtact tttttcgcaa cgttgaccag ctcgctttta
    12601 acagcggtct cgatgctgtc agcagcgata gtaatcgtta cacggcggcc aaggccttga
    12661 gtggtttcaa ctgaaacttg catcttgtta cctcaaaaaa tcacagtgct cggtcaactc
    12721 ttaccccgca agcacaggtt gttggcttcg cggaaccggg atgttcttat aatcaatcac
    12781 attccctgtt accagaatca tcccgaaagc attcaaatag gacgcggcat tatagcggca
    12841 tcacttttat gagtcgagaa cggttattgc gcgctgtcgc gtattttttc acaattcccg
    12901 ggcaattttc gtctgaatgc tgggcaaaaa gcgcaaaatc cagacaaaac aaaacggccc
    12961 gcaggccgtt tttcataaaa tctttagcaa catactggaa aagcgccgac agttgcaaat
    13021 gcgtttttac gcgatgcttc ctgctccacg acagggagga gaggcaaaga cggtgtcctg
    13081 caacccttcc cactccttaa tagtgtaagt atgcagagcc agcgcatgaa cggtagtaga
    13141 gagttcctcc gctaaagtac tgtaaatcat tcgatgacga ttcagaaaac gttcacccgt
    13201 aaaacgatcg ctgaccagca caactttaaa atggctttca gagccggctg ggacattgtg
    13261 acgatagctt tcatccacta cttcgaggaa tacgggttgg aacgccgccc ttaatttttc
    13321 ttctatccgc tcacgtatca tcatgaaata tctcctccga caacgctgag atgtcatcca
    13381 tccctttaaa tactagccgc ttttaccgtt tccattgcag cttaacaaca aatatttagg
    13441 tttcgtcctg gtttttccgt caagcgtatg aagtttactg atttaaagat tgaaacacag
    13501 gcctcgcatc agcgtttctt ttaccagggg tgattatttc gtcaacatga tgaatttaca
    13561 tgccttaccc acttcccctc gccgttggca atgttatgat ggcgggaatt tttaccaaac
    13621 acgcttaaga gtctctgaga gcccgaacat gttcaaaaaa atcctcttcc cgttagttgc
    13681 tctgtttatg cttgcaggat gcgcaaaacc gccaacaact attgaagttt ccccgacgat
    13741 tacactgcca cagcaggatc caagcctgat gggcgtcacc gtaagcatta atggtgccga
    13801 tcagcgtacc gatcaggcgc tggcaaaagt cacccgcgat aatcaaatcg ttaccctgac
    13861 cgcctcccgc gctctgcgtt tcctgctgca agaagtgctg gaaaaacaga tgaccgcgcg
    13921 tggttacatg gttggtccga atggcccggt taatctgcaa atcatcgtta gccaactgta
    13981 tgctgacgtg tcccagggca acgtgcgcta caacatcgct accaaagcgg atatcgccat
    14041 catcgctacc gcgcagaatg gcaacaaaat gaccaaaaac tatcgtgcca gctacaacgt
    14101 tgaaggtgcg ttccaggcct ccaacaaaaa tatcgccgat gcggttaaca gtgtgctgac
    14161 cgataccatc gctgatatgt ctcaggacac cagcattcac gaattcatca agcagaacgc
    14221 gcgttaattt ctgccctctg gcccggtgca agccgggcct gtagacgccc atgtccagtc
    14281 aatatttacg tatttttcaa cagccgcgtt cagccatatt gctgatcctg ggttttgctt
    14341 ccgggctacc gctcgccctg acatccggca ccttacaggc ctggatgacg gtcgaaaata
    14401 tcgatctcaa aaccattggt ttcttctctc tggtaggcca ggcttacgtt tttaaattcc
    14461 tctggtcacc gctgatggac cgctacacgc ccccattttt tgggcggcgg cgcggttggc
    14521 tgctcgccac gcaaatcctg ttattagtcg ccattgcggc gatgggtttt ctcgaaccag
    14581 gcacccaact ccgctggatg gcggcgctgg cggtggtgat cgctttttgc tctgcctccc
    14641 aggatattgt cttcgatgcg tggaaaaccg atgtgctccc ggcagaagaa cgtggtgcgg
    14701 gcgcggcaat cagcgtgctg ggttaccgtt tagggatgct ggtttccggc ggcctagccc
    14761 tgtggctggc agataaatgg ttgggctggc agggcatgta ctggttgatg gcggcgctgt
    14821 tgatcccctg tattatcgcg acgttgcttg caccagaacc aaccgacacc attcctgtgc
    14881 ccaaaacgct ggaacaagcg gttgttgcac ctctgcgaga tttctttggt cgcaataatg
    14941 cctggcttat tttgcttctt atcgtgctgt ataagctggg cgacgcattc gccatgagcc
    15001 tgacaaccac gtttttgatt cgcggcgtcg ggtttgatgc gggtgaagta ggcgtggtaa
    15061 acaaaacgct tggcttactg gcgaccattg ttggcgcatt gtacggtggg attttgatgc
    15121 agcgcctgtc gctgttccgg gcgctgctga ttttcggcat tttacaaggt gcgtctaacg
    15181 ctggttactg gctgctgtcg attactgata agcatctcta cagcatgggc gcagccgtct
    15241 ttttcgaaaa cctctgtggc gggatgggca catcagcctt tgtcgcgctg ttaatgacgc
    15301 tgtgtaataa gtcattttcc gctactcaat ttgccctgct ctcagcgctt tccgctgtag
    15361 ggcgagttta tgtcggcccc gtggcgggtt ggtttgttga agcacacggc tggtcgacat
    15421 tctatctatt ctccgtcgcc gctgccgtac cggggcttat tttgctgctg gtttgccgcc
    15481 agacgcttga atatacacga gtaaatgaca actttatctc ccgtaccgaa tatccggcag
    15541 gttatgcctt tgccatgtgg acactggcgg cgggcgtcag cctgttggcc gtgtggttac
    15601 ttctgttgac gatggacgcg ctggatttga cgcacttctc tttcctgcct gctctgctgg
    15661 aagtcggggt tttagtcgcc ctttctggcg tcgtgcttgg tggtttgctg gattatctgg
    15721 cgctacgaaa aacgcatctg acgtaatctg taaatattat ttagctgtag taatcactcg
    15781 ccgtaattat tacggctaaa taaacatcag catcgcttat taatatttgt tcttttgtgc
    15841 ggcttagcgt ttggaaatta ttgacgccat ttataaattc tatttttctt acacgattca
    15901 gctaatgagt ctttattttc tcaccaccca gttgtcactt taatgataat tatttgttaa
    15961 ataattgttt tatttcgcat tggttatacc aattgcccac caagactcct tctgcactcc
    16021 ccttttgtta
//
LOCUS       DAZXUK010000076        15959 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZXUK010000076 DAZXUK010000000
VERSION     DAZXUK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15959
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.076"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..2264
                     /locus_tag="ACH3L8_001898"
                     /pseudo
     CDS             1..2264
                     /locus_tag="ACH3L8_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2265..3914
                     /locus_tag="ACH3L8_001899"
     CDS             2265..3914
                     /locus_tag="ACH3L8_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFT4085035.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3919..4695
                     /locus_tag="ACH3L8_001900"
     CDS             3919..4695
                     /locus_tag="ACH3L8_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFT4085036.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4970..7819
                     /gene="rcsC"
                     /locus_tag="ACH3L8_001901"
     CDS             4970..7819
                     /gene="rcsC"
                     /locus_tag="ACH3L8_001901"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFT4085037.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="ACH3L8_001902"
     CDS             complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="ACH3L8_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFT4085038.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="ACH3L8_001903"
     CDS             complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="ACH3L8_001903"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFT4085039.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            12096..13217
                     /gene="ompC"
                     /locus_tag="ACH3L8_001904"
     CDS             12096..13217
                     /gene="ompC"
                     /locus_tag="ACH3L8_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFT4085040.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13329..14384
                     /gene="apbE"
                     /locus_tag="ACH3L8_001905"
     CDS             13329..14384
                     /gene="apbE"
                     /locus_tag="ACH3L8_001905"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFT4085041.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14458..15522
                     /gene="ada"
                     /locus_tag="ACH3L8_001906"
     CDS             14458..15522
                     /gene="ada"
                     /locus_tag="ACH3L8_001906"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFT4085042.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15522..>15959
                     /locus_tag="ACH3L8_001907"
     CDS             15522..>15959
                     /locus_tag="ACH3L8_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HFT4085043.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSV"
BASE COUNT         3895 a         3896 c         4275 g         3893 t
ORIGIN      
        1 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
       61 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
      121 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct atgaccaggc
      181 gctctccagc gagccggttg cgcctggcgc gactaaccgc agcgagcggc gagtaaaaat
      241 gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
      301 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
      361 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
      421 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgacaa
      481 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
      541 taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat
      601 ttcgctgacg cagaatattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
      661 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
      721 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
      781 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
      841 tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc tgatcccgct
      901 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
      961 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     1021 ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat ggtacgccga
     1081 ctggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc agcatatgct
     1141 cgacagttat gccgagcagg cggataatat gccgttattg catcgggcgc tggtgctggc
     1201 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaaggggggg atgaagctat
     1261 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     1321 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     1381 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     1441 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     1501 tagcggtggt gacgctaccc agacggccgc tattttaatg gtttgaccgc tgagcaatcc
     1561 actattgagc gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca
     1621 gttgttttgc ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa
     1681 gactggcgtt gggttggtta gggtgtgccg gacattctct cttttggtga cgaattatcg
     1741 ccgcaaatgt gcaggtccgc tggcgtgaac cggcaaaaac ggctcaacaa agtaacattc
     1801 cggtgaccgt tgaacgccag ttgtatcggc ttatccccgg tgaagaagag atgagcttta
     1861 ctctgcagcc ggtgaccagc aatgagattg acagcgatgc gctgtatctc gatgaaatta
     1921 cgcttaccag cgagcaggat gcagttctgc gctatggtca ggtggaagta ccgctcccgc
     1981 cgggagctga cgttgagcgc acaacatggg gcatttcagt caataaaccc aacgccgcga
     2041 aacagcaggg gcaattgctg gaaaaagcgc gtaatgaaat gggcgaactg gcctatatgg
     2101 tgccggtgaa agaactgacg ggaacggtca ctttccgcca tttgctgcgc ttctcgcaaa
     2161 aagggcaatt cgttctgcct cctgctcgtt atgtgcgttc ctatgcacct gcgcagcaaa
     2221 gtgtcgcggc agggagtgaa tggaccggga tgcaggtgaa ataagtgaac tggcgcagaa
     2281 tcgtctggct attggcgttg gttactctgc caacgcttgc ggaggagacg ccgttacaac
     2341 tggtgctgcg cggtgcgcag cacgatcaac tgtatcagct gtcgtcatca ggcgtgacca
     2401 aagtctcagc attacctgac tcgctcacca cgccgttagg gagtttgtgg aaactgtatg
     2461 tttacgcctg gctggaggat acccatcaac cggagcagcc ctaccagtgt cgcggaaact
     2521 ccccggaaga ggtttattgt tgtcaggctg gggagagcat aacgcgcgat accgcgctgg
     2581 tacgttcctg tgggctctat tttgcgccgc agcggttgca tattggcgca gatgtgtggg
     2641 ggcaatactg gcaacagcgt caggcacctg cgtggttagc gtctcttaca acgctgaagc
     2701 cagaaacctc ggtaacggtt aaatcgctgc ttgattcatt agcgacgtta cctgcgcaaa
     2761 acaaagctca ggaagtctta ctggatgtgg tgctggacga ggcgaaaatc ggtgtcgcat
     2821 ccatgctcgg tagtcgggta cgggtgaaaa cctggagctg gtttgccgat gataaacagg
     2881 aaatccgtca gggtggtttt gctggctggt taaccgatgg tacaccgctg tgggttactg
     2941 gcagtggcac cagcaaaacg gtactaaccc gttatgcgac ggtgcttaat cgggttttgc
     3001 cagtgccgac gcaggttgcc agtgggcaat gtgtagaggt ggagctgttt gcccgctatc
     3061 cgctgaagaa aatcacagcg gaaaagagta cgacggcagt aaaacccagc gtacttaatg
     3121 ggcgctatcg tgtgacgttt actaatggta accacattac gtttgtcagc cacggtgaga
     3181 cgaccttact gagcgaaaag ggcaaactga aattgcagtc gcatctcgat cgtgaagagt
     3241 acgttgccag agttctggat cgcgaggcga aaagtacgcc acctgaggca gcgaaagcca
     3301 tgactgtggc gatacgtact tttttacagc aaaacgccaa tcgggaaggt gattgcctga
     3361 ccatccccga cagcagcgcc acgcagcgtg tttctgcttc gccagcgacc acgggggcgc
     3421 gaacgatgac cgcctggacg caggagctca tctatgcagg cgatccggtt cattatcatg
     3481 gcagtcgcgc taccgaaggg acactttcct ggcggcaagc cacggcccag gcggggcagg
     3541 gggagcgtta cgaccagata ctcgcgtttg cttatcccga caacagcctt agccgctggg
     3601 gagctccacg ctcaacctgc cagttattac ccaaagcgaa agcctggctg gcgaaaaaaa
     3661 tgccgcagtg gcggcgtata ttacaaggcg agacggggta caacgaacca gacgtgtttg
     3721 cggtctgtcg tttagtctca ggtttcccct ataccgatcg gcagcagaaa cggctgttta
     3781 ttcgtaactt ctttacgctt caggatcggc tggatttaac ccatgagtat ctgcacttgg
     3841 cctttgatgg ttatcccacc gggcttgatg agaactatat cgaaacgctg acccgacaat
     3901 tattaatgga ctaatgctat gcgaaagatt tttcttccgt tgttactggt ggcactttcg
     3961 cctgtcgctc acagtgaagg tgtgcaggaa gtcgagattg acgcaccgct ctctggctgg
     4021 catccggcag agggcgaaga tgccagtttt tcacaatcga tcaattaccc ggcgtcgtcc
     4081 gtcaacatgg cagatgatca aaatatctca gcgcagatcc gcggcaaaat aaagaattat
     4141 gctgcggcgg gtaaagttca gcagggccgg ctggtggtca acggtgccag tatgccgcag
     4201 agaattgaat ccgatggttc atttgcacgc ccttatattt tcactgaagg cagcaacagc
     4261 gtgcaggtca tcagcccgga tgggcaaagc cggcaaaaaa tgcagtttta ctcaacgccg
     4321 ggtacaggaa ctattcgtgc acgtttacgg ctggttctct cgtgggatac ggacaatacc
     4381 gatctcgatc tccatgttgt cacgcccgat ggcgaacacg cctggtacgg taacaccgtg
     4441 ctgaaaaaca gtggtgcact ggatatggat gtcacgacgg ggtacggacc cgagattttc
     4501 gccatgccag caccagttca cgggcgctat caggtgtata tcaactacta tggtgggcgc
     4561 agcgaaacgg aattgacgac cgcccaactg acgctgatca ccgatgaagg atcggtcaat
     4621 gagaaacagg aaacatttat tgtaccgatg cgtaatgctg gcgagctgac gctggtgaaa
     4681 agttttgact ggtgatccat cccttcacac aacctggctc tctctttttt cagataagac
     4741 tgctgggcga tctgccgacg ccaggatata accattaaca agcgtcggca gattggtcat
     4801 ttcttatttt tcgtactgag aactgtccat ctactccggc tttcctggtt aaggcaatgg
     4861 tttctaagcg atacgcatta ttgcaatggt gacagaacat tgcggtaaca cgcttttacc
     4921 gctaccttaa ccacactcca tcggtcacct gaggcggagc ttctcccctt tgaaatacct
     4981 tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg ttcagagcat tggcgttagt
     5041 gctctggctg ttgattgctt tttcatccgt tttttacatc gttaatgcgt tacatcagcg
     5101 agaatcggaa attcgtcagg aatttaatct gagttccgat caggctcagc gctttattca
     5161 acgcacctct gatgtgatga aagagcttaa gtacatcgcc gaaaatcgct tatcggcaga
     5221 aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg gatgtgcctg cgtttgaacc
     5281 gctgtttgcc gactccgatt gttccgcaat gagtaacacc tggcgaggtt ctctggagtc
     5341 attggcgtgg tttatgcgct actggcgcga taatttttct gcggcttacg atctcaaccg
     5401 ggtattttta atcggcagtg ataacctctg catggccaat ttcggtctgc gtgatatgcc
     5461 agtggaacgc gataccgcgt tgaaagcgtt gcatgaacgc atcaataaat atcgaaatgc
     5521 accacaagat gatagcggca gtaacctcta ctggatcagc gaaggtccgc gcccttgcgt
     5581 cgggtatttt tacgcgttga cgccagttta tctggcgaac cggttgcagg cgcttttggg
     5641 tgtcgagcag accatccgga tggagaactt tttcttaccg ggtacgttgc cgatgggggt
     5701 taccattctt gatgaaaatg gtcataccct gatttcgctt accggaccag aaagtaaaat
     5761 taagggcgat cctcgctgga tgcaggaacg ctcctggttt ggctatacgg aagggttccg
     5821 ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta agcatcgtgt attcggtgcc
     5881 ggttgataag gtgctggaac gcattcgcat gttgatcctt aacgcaattt tgctgaatgt
     5941 gcttgccgga gctgcattgt ttactctcgc gcggatgtac gagcgacgta ttttcattcc
     6001 ggcggaaagc gacgccctgc gactggaaga acatgagcag ttcaatcgca agattgtcgc
     6061 ctccgcgcca gtgggtatct gcattttgcg taccgctgat ggcgtcaata ttttaagtaa
     6121 cgaactggcg catacctatc tcaatatgct tacgcatgag gaccgccaac gactgacgca
     6181 aattatctgt gggcagcagg tcaattttgt tgatgtcctg accagcaaca ataccaatct
     6241 gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac gtggccattt gtgtgctggt
     6301 ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag gagatggcac aagcagcgga
     6361 acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc agtcatgagc tgcgaacgcc
     6421 gctgtatggc attatcggta acctggatct gttgcaaacc aaagagttac cgaaaggcgt
     6481 cgatcgtctg gtgacggcaa tgaacaactc ttccagcctg ttgttgaaaa ttatcagcga
     6541 tattctcgat ttctcgaaga ttgaatcgga acagttgaag atcgaaccgc gtgagttttc
     6601 accgcgtgaa gtgatgaacc acatcaccgc caactattta ccgctggtgg tacgcaagca
     6661 gttaggcttg tactgcttta ttgaaccgga tgtgccagtg gccttaaatg gcgacccgat
     6721 gcgtttacag caggtcatct ccaacctgtt gagtaacgcc ataaaattca ccgataccgg
     6781 ctgtatagtt ttgcatgttc gcgctgatgg cgattatctc tctatccgtg ttcgcgatac
     6841 cggcgtgggg attccggcga aagaagtggt gcgcttgttt gatcccttct tccaggttgg
     6901 aacgggcgta cagcgaaact tccaggggac cggtctgggt ctggcgattt gtgaaaaact
     6961 gatcagcatg atggacggcg atatctcggt agattcagaa ccgggaatgg gcagccagtt
     7021 taccgtgcgt attccgttgt acggcgctca gtacccgcag aaaaaaggcg tggaagggtt
     7081 gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg ctctgtcagt tcctggaaac
     7141 cagtttgcag cgcagcggca tcgtcgttac aacatacgaa gggcaggaac cgactcccga
     7201 agatgtgtta atcactgacg aggtagtgag taaaaaatgg cagggcagag cggtagtgac
     7261 cttctgtcgt cgccatattg gtattccgct ggagaaagcg ccaggggagt gggtacacag
     7321 tgtggctgct ccgcatgagc taccggcatt gttggcgcgt atttatttga tcgagatgga
     7381 gagcgacgat cctgctaacg ctctgccgtc gacggacaaa gcggtcagcg ataacgacga
     7441 tatgatgatt ctggtcgtgg atgatcatcc gattaaccga cgtctgctgg cagatcagtt
     7501 gggatcgttg ggctatcaat gtaaaaccgc gaatgatggc gtcgatgcgc ttaatgtact
     7561 tagtaagaat catattgata tcgtgcttag cgacgtcaac atgccaaata tggatggtta
     7621 ccgcttgacg caacgcattc gtcagttggg actgacgttg ccggtaatcg gagtaactgc
     7681 taatgcgttg gctgaagaga agcagcggtg tctggagtcc ggtatggaca gctgcctctc
     7741 gaagccggta acgctggatg tgataaaaca gacgctgacg gtatatgccg agagggtcag
     7801 gaaatcgcgg gattcgtagg acggataagg cgtttacgcc gcatccggca ttcagtgcaa
     7861 atgccagata agacactgac gcgtcttatc tggcctactt taatgtaatc tgttgaaata
     7921 atgggaatcg taggccggat aaggcgttta cgccgcatcc ggcaatcggt gcaaatgcca
     7981 gataagacac taacgcgtct tatctggcct acagatgatt agtctttatc tgccggactt
     8041 aaggtcacag aagagagata attcagcagg gcgatatcgt tctcgacgcc cagcttcatc
     8101 atcgcagatt tcttctggct actgatggtt ttaatactgc ggttcagctt tttagcgatc
     8161 tcggtcacta ggaagccttc cgcaaacagg cgcagaactt cactctcttt tggcgagaga
     8221 cgcttgtcac cgtaaccacc agcactgatt ttttccaaca ggcgagaaac gctttccggg
     8281 gtaaatttct tccctttctg cagcgcggcg agagctttcg gcagatcggt cggtgcacct
     8341 tgtttcagca cgatcccttc gatatccaga tccaataccg cactaagaat cgccgggttg
     8401 ttgttcatag tcagaacaat gatcgacagg cttgggaaat ggcgcttgat gtacttgatt
     8461 aaggtaatgc catcgccgta cttatcgcca ggcatggaga gatcggtaat caacacatgc
     8521 gcatccagtt tcggcaggtt gttgatcagt gctgtagagt cttcaaattc gccgacaaca
     8581 ttcacccact caatttgctc aagtgatttg cgaataccga acaagactat cggatggtca
     8641 tcggcaataa ttacgttcat attgttcatg taataggcta ccttgctaca gcaagctctt
     8701 gacataactg tcaatgtcgc tgatgtattt ttctattcct ggaacatcct tctcacgaat
     8761 cagatgttcc agcgtttcac ataactgctt gccgggtacc agatttagca tggcaaatac
     8821 gcctttaaga cgatgagccg tttgggctaa cgcagcaaag tcactggttg ctgcttcagt
     8881 atacagcctc ttaacatcat ccggtactgt gtctacaaag agcgcataat agccgctggc
     8941 atggagttgc gcattttcat ctccgcccag aggcgattct gtcacctctt cctgtgccag
     9001 ttgcacttca attaattgta agaccgcttc ctgcatagcg ttgctcatat tgaagttgac
     9061 gcacaattga ccaggcccaa tttctcgtac gccagactca tcatcgctta aaagcaagcc
     9121 agaggcagta agattagacg gattatccgt taaaaagata tcataatctt gactaattaa
     9181 tctttcatcg ggtgtgatac aggttgcacc ccaattttct aacaggcgag tgacaatatt
     9241 ccgaatttct gccgaagtaa catccaccat tacgcagaca tcatccagta aacgctcttc
     9301 ttcctcttca acttccgggt cagcggcgag cattttgata tgcacagagt agcgtgtacc
     9361 aagcccatcc cgcgttttga tgtttaaatg accgcccagt ttacgtgcca gttgatcgct
     9421 cagccagaat gccagcgggt ccgcctttcc ataacgatcg ttttgggtct ggttgataaa
     9481 cgggaagtgc aaattatcca tttcatgaat acttacgcct tctcccgtgt ccagaatgcg
     9541 gaatgtcagg cggtcttcag aggactcatc ctgatcaacc tcaagggtga tttttcccaa
     9601 ttgcgttgag gtcacggcat attgcatcag tagcagcaaa atacgtcgta aggcatcgcg
     9661 atcgccgcgg cgcatatcgt gtgctttcag atgattgtta atcagcagtt gcagaccttt
     9721 acgcttgatg gcaggcaaca ctgaaggcac aacttcatca attaaatcct gcacggagaa
     9781 cagcaccgtc tcacttttcc agctatcatc cgcaagcatg ttcgctaact gtatttcttc
     9841 gaccagccgc accagcacat ctgcctgatt cgccagttgt ttgctttccg gggcgttgag
     9901 tttagccgca ctctccgcca gggactgtgc gggttctttc agcgcatcgc caatgttttt
     9961 cataaaggtc atccgcccct gctggttttt ctcatacaga cgctgcgcct gcttgagttt
    10021 cttgtttacc agcacttcgc gatcctgatc gcgaataatg aaaatttgtg tgcgcggcgc
    10081 gacctggctg cggaacatgc ggatctcata cagctcgtta ttgatcgtcg cctgaataat
    10141 cccctgatgc tgttccgcca tggtggtgat gttttgcaga ttcagatgcg gcagcaaatg
    10201 atcggcaatt ttgttactta tgacagtgcg gttcgattcc tgatcgtgaa ccagcaggcc
    10261 gagcggcaac agtgagacta tctcttcatt gattgcccgt aaaatgcgca attcgttatt
    10321 gaccgccgtg ctggggacgc tttctgtact gcggctggag aaatggcgga atgtggtata
    10381 gccaaataac gccagcgcca gcaaaccgat gttcagcagc agtggcagca gaatgttttg
    10441 caacgtatcc agcaataagg tgccataagg aacctgccag accaggcgca tatcggtaga
    10501 gttgagtgcc gaggagattt caatcttcgt actgttaaag tggatactga cgctatccgt
    10561 cccttctttc tcattatcat tgtttcccgt tgccgtcgcg tctggctcaa ggcggaaact
    10621 gtccagcggc atacccggtg ggatcaaatc attaatcggc agatcaaaag ccacgaccgt
    10681 tgccagatgt cccggctggt tgaacgtggt acgcaaggta aagtaatgac cgttctgcca
    10741 ggctaggcgg cgcatgttag aaaaactttc gcgttcatcg agggcgttgg cctgttgtaa
    10801 catttctgca cgacgtgaat caacaatgtc actgacggtc gattctttaa atccggaggt
    10861 gagatcttta aggggtaggg ttgagatcag caccagactg ttatcctgac cattcaggta
    10921 atacatcgac cacggtacat tttctgcacc ccacaatgta tccagataag tggacatccg
    10981 ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag attaacgctt ccgttttgcg
    11041 gcgtggtttc tccagatagt agacatcctg tttcaggcgc gtctcttgta aaccttcgcc
    11101 ggaggagggg gaggtcgtcg cggcaatgtt gtcgtagatc tgcctggtca cgtaacgcca
    11161 ggtatcgacg cgcttttgga ttgcgtgggt aatgtcgaca atctggtaac ttttatcttt
    11221 cagccaagcg ttaacggcac tttgtaccat tacccccatc gtcaccagta acacaatgat
    11281 caacagtaaa aagaagcggg taatgctccc cggtaggagt gaaaagcggg tcgtggccgt
    11341 tgtctctttc tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag tgatagggca
    11401 gtataaaggg tacagtgtga aattccagac tcttacctga ttgatgattc tctattctcc
    11461 cgtgatattt atcatcctgt atggggggaa atagatgtac aagcgccatt ttgctgtaca
    11521 aataacctac aaaaagccca acgaaattaa ttgcaaataa acaaagattg ctggaaatta
    11581 tgctgatgtt aattatttgt gaaatagtta acaagcgtta tagtttttct gtggtagcac
    11641 agaataatga aaagtgtgta aagaagggta aaaaaaccga atgcgaggca tccggttgaa
    11701 ataggggtaa acagacattc agaaatgaat gacggtaata aataaagtta atgatgatag
    11761 cgggagttat tctagttgcg agtgaaggtt ttgttttgac attcagtgct gtcaaatact
    11821 taagaataag ttattgattt taaccttgaa ttattattgc ttgatgttag gtgcttattt
    11881 cgccattccg caataatctt aaaaagttcc cttgtattta cattttgaaa catctatagc
    11941 gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt
    12001 tggggagaat ggacttgccg actgattaat gagggttaat cagtatgcag tggcataaaa
    12061 aagcaaataa aggcatataa cagagggtta ataacatgaa agttaaagta ctgtccctcc
    12121 tggtcccagc tctgctggta gcaggcgcag caaacgctgc tgaagtttac aacaaagacg
    12181 gcaacaaatt agatctgtac ggtaaagtag acggcctgca ctatttctct gacgacaagt
    12241 ctgtagatgg cgaccagacc tacatgcgtc ttggcttcaa aggtgaaact caggttactg
    12301 accagctgac cggttacggc cagtgggaat atcagatcca gggcaacagc gctgaaaacg
    12361 aaaacaactc ctggacccgt gtggcattcg caggtctgaa attccaggat gtaggttctt
    12421 tcgactacgg tcgtaactac ggcgtagttt acgacgtaac ttcctggacc gacgtactgc
    12481 cagaattcgg tggtgacact tacggttctg acaacttcat gcagcagcgt ggtaacggct
    12541 tcgcaaccta ccgtagcacc gacttctttg gtctggttga cggtctgaac ttcgctgttc
    12601 agtatcaggg caaaaacggt agcccggaag gtgaaggcat gaccaacaac ggtcgtgaag
    12661 ctctgcgtca gaacggtgac ggcgttggcg gttctattac ttatgattac gaaggcttcg
    12721 gtatcggtgc tgcagtttcc agctccaaac gtactgatga ccagaacttc ggtctgaacc
    12781 gttatgatga gcgttacatc ggtaatggcg accgcgctga aacctacacc ggtggtctga
    12841 aatacgacgc gaacaacatc tacctagctg ctcagtacac ccagacctac aacgcaactc
    12901 gcgtaggcaa cttgggttgg gcgaacaaag cacagaactt cgaagctgtt gctcagtacc
    12961 agttcgactt cggtctgcgt ccgtccctgg cttacctgca gtctaaaggt aaaaacctgg
    13021 gtgtcatcaa tggtcgtaac tacgacgacg aagatatcct gaaatatgtt gatgttggtg
    13081 cgacctacta cttcaacaaa aacatgtcca cctacgttga ctacaaaatc aacctgctgg
    13141 acgacaacca gttcactcgt gacgctggca tcaacactga taacatcgta gctctgggtc
    13201 tggtttacca gttctaatct cgatggatat cgaacaaagg gcctgcgggc ccttttttca
    13261 ttgttttcag cgtacaaaca cagttttttg gtgtactctt gcgaccgttc gcatgaggat
    13321 aatcacgtat ggaaataagc tttacccgcg tggcactgct ggttgccgcg ctcttctttg
    13381 ttggttgcga tcaaaaacca caacccgcca aaacccacgc tactgaagtt accgttcttg
    13441 aaggcaaaac catgggtacc ttctggcgtg ccagcatccc gggcattgat gccaaacgca
    13501 gtgccgaact taaagaaaag attcagaccc agctggacgc cgacgatcag ctgctttcga
    13561 cctataaaaa agattccgcg ctgatgcgct ttaacgactc gcaaagtttg tcgccgtggc
    13621 cggtaagtga agcgatggcc gatatcgtca ccacctcgct gcgcattggg gcaaggaccg
    13681 atggtgcgat ggatattacc gtcgggccgc tggtgaatct gtggggcttt ggcccggaac
    13741 aacagccggt tcaaattccg agccaggaac agatcgatgc gatgaaagcc aaaaccggct
    13801 tacagcacct gacggtcatc aatcagtcgc atcagcaata tctgcaaaaa gacctgccgg
    13861 atttatatgt cgatctctcc accgtcggtg aaggttatgc ggcggatcac ctggcacgct
    13921 tgatggagca ggaagggatt tcccgctatc tggtgtcggt gggcggcgcg ctgaacagcc
    13981 gtggtatgaa cggtgaaggc cagccgtggc gggtagcgat tcaaaaacca accgataaag
    14041 aaaacgcggt tcaggctgtg gtggatatca acggtcatgg gattagcacc tctggcagct
    14101 accgtaacta ttacgaactg gacggcaaac gtctttccca tgttatcgat ccgcaaaccg
    14161 ggcgtcccat cgaacacaat ctggtatccg tgacggtgat tgctccgacg gcgctggaag
    14221 ccgatgcctg ggatactggc ttgatggtac tcgggccgga gaaagccaaa gaggttgttc
    14281 gccgggaagg gctggcggtt tatatgatca ccaaagaagg cgatagcttt aaaacctgga
    14341 tgtcaccaca gtttaaaagc ttccttgtca gcgaaaaaaa ttaaagcgca agattgttgg
    14401 tttttgcgtg atggtgaccg ggcagactaa aggctatcct taaccaggga gctgattatg
    14461 aaaaacgcca catgcttaac tgacgatcaa cgctggcaat ctgtcttagc ccgcgacccg
    14521 aatgccgacg gcgaattcgt tttcgccgtg cgcaccacgg gtatcttttg ccgtccttct
    14581 tgccgcgcca gacatgcctt gcgggaaaat gtctccttct acgcaaatgc cagcgaggca
    14641 ctcgccgccg gatttcgccc ctgcaaacgt tgtcagccag acaaagccaa tccccggcaa
    14701 catcggttgg ataaaatcac ccacgcgtgt cgactgctgg aacaggaaac gcctgtaacg
    14761 ctggaagcct tagccgacca ggtggcgatg agtccatttc atctacatcg gttgtttaaa
    14821 gctactaccg gaatgacgcc taaagcctgg caacaggcct ggcgcgctcg ccgtttgcgc
    14881 gaatcgctgg cgaaagggga gagcgtgacg acgtctattc ttaacgccgg attccccgac
    14941 agcagcagtt actaccgcaa agccgatgaa acgctgggca tgacggctaa acaattccgt
    15001 cacggtggcg aaaatctggc ggtgcgttac gcgctggctg attgtgagct gggtcgttgc
    15061 ctggtggcag aaagcgagcg ggggatttgc gcgatattgc tgggcgatga tgacgcgaca
    15121 ttaatcagcg agttgcagca gatgtttccc gctgccgaca acgcgcctgc cgatctgacg
    15181 tttcagcaac atgtgcgtga agtgatcgcc agcctcaatc aacgcgatac gccgctgacg
    15241 ttaccgctgg atattcgcgg cactgctttt cagcaacaag tctggcaggc actgcgcacg
    15301 ataccttgtg gtgaaaccgt cagttatcag caactggcta acgccatcgg caaaccgaaa
    15361 gcggtacggg ccgttgccag cgcctgtgcc gccaacaagc tggctatcgt aataccctgt
    15421 catcgggtgg tccgtggtga tggcacactt tccggttacc gctggggcgt gtcgcgtaaa
    15481 gcgcaactgc tgcgccgcga agctgaaaat gaggagaggt aatgttggat ctgtttgccg
    15541 atgctgaacc gtggcaagag ccactggcgg ctggtgcggt aattttacgg cgttttgctt
    15601 ttaatgctgc ggagcaacta atccgcgata ttaatgacgt tgccagccag tcgccgtttc
    15661 gccagatggt cgctccaggc ggatatacca tgtcggtggc gatgaccaac tgtgggcatc
    15721 tgggctggac gacccatcgg caaggttatc tctattcgcc cattgatccg caaacaaata
    15781 aaccgtggcc cgctatgcca caaagctttc atgatttatg tcaacgtgcg gctacggcgg
    15841 cgggctatcc agatttccag ccagatgctt gtcttatcaa ccgctacgct cctggcgcga
    15901 aactgtcgct gcatcaggat aaagacgaac cggatctgcg cgcgccaatt gtttctgtt
//
LOCUS       DAZXUK010000077        15646 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZXUK010000077 DAZXUK010000000
VERSION     DAZXUK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15646
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.077"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            89..607
                     /locus_tag="ACH3L8_001908"
     CDS             89..607
                     /locus_tag="ACH3L8_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFT4085044.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            597..1822
                     /gene="dgcN"
                     /locus_tag="ACH3L8_001909"
                     /pseudo
     CDS             597..1822
                     /gene="dgcN"
                     /locus_tag="ACH3L8_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            1838..2320
                     /locus_tag="ACH3L8_001910"
     CDS             1838..2320
                     /locus_tag="ACH3L8_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFT4085045.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(2397..2744)
                     /gene="rplS"
                     /locus_tag="ACH3L8_001911"
     CDS             complement(2397..2744)
                     /gene="rplS"
                     /locus_tag="ACH3L8_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFT4085046.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2786..3553)
                     /gene="trmD"
                     /locus_tag="ACH3L8_001912"
     CDS             complement(2786..3553)
                     /gene="trmD"
                     /locus_tag="ACH3L8_001912"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFT4085047.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3584..4132)
                     /gene="rimM"
                     /locus_tag="ACH3L8_001913"
     CDS             complement(3584..4132)
                     /gene="rimM"
                     /locus_tag="ACH3L8_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFT4085048.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4151..4399)
                     /gene="rpsP"
                     /locus_tag="ACH3L8_001914"
     CDS             complement(4151..4399)
                     /gene="rpsP"
                     /locus_tag="ACH3L8_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFT4085049.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4536..5897)
                     /gene="ffh"
                     /locus_tag="ACH3L8_001915"
     CDS             complement(4536..5897)
                     /gene="ffh"
                     /locus_tag="ACH3L8_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFT4085050.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6064..6855
                     /locus_tag="ACH3L8_001916"
     CDS             6064..6855
                     /locus_tag="ACH3L8_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFT4085051.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6876..8162
                     /locus_tag="ACH3L8_001917"
     CDS             6876..8162
                     /locus_tag="ACH3L8_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFT4085052.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8217..8810)
                     /gene="grpE"
                     /locus_tag="ACH3L8_001918"
     CDS             complement(8217..8810)
                     /gene="grpE"
                     /locus_tag="ACH3L8_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFT4085053.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8933..9811
                     /gene="nadK"
                     /locus_tag="ACH3L8_001919"
     CDS             8933..9811
                     /gene="nadK"
                     /locus_tag="ACH3L8_001919"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFT4085054.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9897..11558
                     /gene="recN"
                     /locus_tag="ACH3L8_001920"
     CDS             9897..11558
                     /gene="recN"
                     /locus_tag="ACH3L8_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFT4085055.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11707..12048
                     /gene="bamE"
                     /locus_tag="ACH3L8_001921"
     CDS             11707..12048
                     /gene="bamE"
                     /locus_tag="ACH3L8_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFT4085056.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12110..12400)
                     /locus_tag="ACH3L8_001922"
     CDS             complement(12110..12400)
                     /locus_tag="ACH3L8_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFT4085057.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12390..12827)
                     /gene="ratA"
                     /locus_tag="ACH3L8_001923"
     CDS             complement(12390..12827)
                     /gene="ratA"
                     /locus_tag="ACH3L8_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFT4085058.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            12998..13480
                     /gene="smpB"
                     /locus_tag="ACH3L8_001924"
     CDS             12998..13480
                     /gene="smpB"
                     /locus_tag="ACH3L8_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFT4085059.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13695..14057
                     /gene="ssrA"
                     /locus_tag="ACH3L8_001925"
     tmRNA           13695..14057
                     /gene="ssrA"
                     /locus_tag="ACH3L8_001925"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14262..15503
                     /locus_tag="ACH3L8_001926"
     CDS             14262..15503
                     /locus_tag="ACH3L8_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFT4085060.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQLMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         3962 a         4112 c         3718 g         3854 t
ORIGIN      
        1 gatgacggtc gcatgaaaaa atgatatttt aatgagcatc ctgaaaaaat atcgatgcat
       61 ttcgagcgaa gatggtgggg atccctgaat gcgtttttct caccgactgt tcttacttct
      121 gattctcctg ctgacgggag caccaatact cgctcaagag ccttctgacg ttgcgaaaaa
      181 cgtgcgtatg atggtttccg gtattgtcag ttacacccgc tggcctgcgc tatcagggcc
      241 accaaagtta tgtatctttt catcttcacg ttttagtacc gccctacaag agaatgccgc
      301 gacatctttg ccttatctgc ctgtcatcat tcacactcaa caagaggcga tgatttcagg
      361 ctgcaatggt ttttattttg gcaacgagtc accgacattc cagatggaat taaccgaaca
      421 atatccgtca aaggcgttat tattaattgc cgaacaaaat accgagtgca ttattggtag
      481 cgccttttgt ctgatcatcc acaataatga cgtcagattt gccgtaaacc tggatgcctc
      541 atcgcgtagc ggtgtaaagg tcaacccgga tgtcttaatg ctcgcacgga agaaaaatga
      601 tggataacga taattctctt aataagcgcc ccacgtttaa aagagcatta cgcaacatca
      661 gtatgaccag catatttatc actatgatgc tgatctggtt gctgctttcc gtgacctcgg
      721 tgctgaccct gaaacagtac gcgcaaaaaa acctggcact gacagcagca acaatgactt
      781 acagtctgga agcagctgtc gtttttgccg atggccctgc agcaactgaa acactggcag
      841 cgctgggcca gcaagggcat tttcaactgc agaagtacgt gataagcagc aaaatattct
      901 ggcatcctgg cattaccccc gtaaggatcc aggcgatact ttcagcaatt tcataagcca
      961 ctggctcttc cctgccccca tcattcagcc gattcgtcac aatggtgaaa ccattggcga
     1021 agtacgctta accgctcgcg acagttcaat cagccatttt atctggtttt cgctcgccgt
     1081 actgaccggt tgtattctgc tggcatcagg catcgcaatt accctcaccc gccatttgca
     1141 caatggcctg gtggaagcac tgaaaaatat caccgatgtc gtacatgatg tgcgttccaa
     1201 ccgcaatttt tcccgacgag tttcggaaga acgtatcgct gagtttcacc gcttcgctct
     1261 cgacttcaac agtctgctgg atgaaatgga agagtggcag cttcgtttac aggccaaaaa
     1321 tgcgcagctt ctgcgtaccg cgctacatga cccattaacc gggctggcta accgcgcagc
     1381 gtttcgtagc ggcatcaata cgttgatgaa caattccgat gcccgaaaaa cgtcggcgtt
     1441 actatttctt gatggcgata atttcaaata tatcaatgat acctggggtc atgcgacggg
     1501 cgatagggtc ttgattgaaa tcgcaaaacg gttagctgaa tttggcgggc tgcgacataa
     1561 agcataccgc ctgggcggcg atgaattcgc tatggtgctc tatgatgtac agtcggaatc
     1621 tgaagtgcag cagatatgct cagcactgac acaaatcttt aatctcccgt ttgatcttca
     1681 taatggccat cagaccacca tgacattaag cattggttac gcgatgacca ttgagcacgc
     1741 ctctgcggaa aaattacaag agcttgccga tcacaatatg tatcaggcca aacaccagcg
     1801 tgccgaaaag ctggtgagat aacaaggata tatcgatatg ataaagcacc tggtagcacc
     1861 cctgattttc acctcactaa tactgactgg ctgccagtcc cctcagggaa agtttactcc
     1921 tgagcaagtc gccgctatgc aatcttatgg atttactgaa tccgccggcg actggtcgct
     1981 gggcttatca gatgccattc tgttcgcaaa aaatgactac aaattgctcc cggaaagcca
     2041 gcaacagatc caaaccatgg cagccaaatt ggcctcgaca gggctaacac atgcccgtat
     2101 ggatggacac accgataact atggtgaaga cagttacaac gaaggcttat cattgaaacg
     2161 ggcgaatgtc gtggccgatg catgggctat tggtggacaa attccacgca gcaatctcac
     2221 cacacagggt ttaggaaaaa aatatcccat agccagtaac aagaccgccc agggccgcgc
     2281 cgagaaccgc cgcgtcgcag tggtgattgc aaccccttaa aacacgagca aacagacaaa
     2341 aaaagggcca gccaattggc cagcccttct taacaggatg tcgcttaagc gaaatcttag
     2401 ttaagacgct ctttgatacg agcagcctta ccagtacgct cacgcaggta gtacagttta
     2461 gctttacgaa cagcaccacg acgtttgaca gaaatgctgt caactaccgg agagtgagtc
     2521 tggaagacac gctcaacgcc ttcgccgttg gaaattttac gaacagtgaa tgcagagtgc
     2581 agaccgcggt tacgaatagc gataaccacg ccctcgaatg cctgcagacg ttttttggaa
     2641 ccttcaacaa cccatacttt cacttccacg gtatcacccg gacggaagga aggtacgtcc
     2701 tgcttcatct gctcttgttc aagttgctta ataatgttgc tcataattta atctcttatc
     2761 ctgggtaaac tgatctctcg ggggcttacg ccatcccatc atgtttatgt tgctgttgtg
     2821 cgtgttccgt tttgaactcc gccagcaacc ttgcttgctc ttcagtcaga gccaggtttt
     2881 ccagaagttc aggtcttcta agccaggtac ggcccagcga ctgtttcaaa cgccagcgac
     2941 gtatctcggc atggttgccc gacagtaaca ctggcggaac ttccatccct tctaacacct
     3001 caggccgcgt atagtgcggg caatccagca atccttcagc aaaggaatct tccgttgccg
     3061 aggcttcatg tcccagtact cccggaataa accgggaaac ggagtcaatc agcgtcattg
     3121 ctggtaactc accaccactg agaacgtaat cgccgattga ccattcttcg tcaatttcgg
     3181 tttggatcac gcgctcatct ataccttcgt agcgaccgca caccagaatc aatttttgat
     3241 tcgttgccag ttcgctgacg cccgcttgat caagcttgcg tccctgtggt gacagataaa
     3301 tcacctttgc gccttcaccc gccgcggctt ttgctgcatg aatggcgtcc cgcaagggtt
     3361 gcaccatcat taacatcccc ggtccgccgc cgtaaggacg atcgtccacg gtacggtgcc
     3421 ggtcatgcgt gaagtcgcga ggactccaac tctggatgct cagcaggcca tttttaactg
     3481 cccggccagt taccccgtaa tcggtaattg cgcggaacat ttcaggaaac aggctaatta
     3541 tgccaatcca catagcgccg ccttttaccg tttatccggt ggtttaaaaa ccaggatccc
     3601 aatctacttc gattgaacga gtagtgagat cgactttctt gataacctgc ccatcgagga
     3661 acggtacgag acgttccttg ataccaaacg catctttcag gtttgcctta atgacgagaa
     3721 cgtcattaga tccggtttcc atcatatcaa cgactttacc gagatcgtag ccttcagtgg
     3781 ttactacctg gcagcccatc aggtctttcc agtagtagtc cccctcttca agctgaggca
     3841 gctgcgatga atccacgaca atttcacaat tcgtcagcag gttcgccgca tcacgatcgt
     3901 caacgccttt cagcttgatg atcatgtcct gattgtggtg cttccagctt tccagctgga
     3961 cttgctgcca ctgacccgcc ttctggataa accagggctg atagtcaaaa atgctttcgg
     4021 cgtcttcggt ggaagaaaac actctgagcc acccacgaat accgtaagac gaacccattt
     4081 ttcccaaaac gatgggatca acaggtgctt gcgcggtgag ttgtttgctc atcatgacca
     4141 ccgtgacaga ttaagctgct ttgtttactt ctttgatcag cgcagcaacg cgatcagaaa
     4201 tagttgcgcc ctggccaacc cagtgagcga tgcgatccag atccaggcga gtgccttctt
     4261 ctttttcgct agcgattggg ttgaagaaac caacgcgctc gatgaagcga ccgttgcgtg
     4321 cattacggct gtcagcgaca acaacctggt agaacggacg ctttttagcg ccgtgacgtg
     4381 ctaaacgaat agttaccata acatcctctt gtgtgaataa aacaaccgga ccccatcgag
     4441 gaacggagtc cggtgtcata ttaaaagccc gaaaatttta ctgatttctg gagaaaatgc
     4501 aatcagcagt taataactct gtcgcataag gtcatttagc gaccaggaaa tcccggcggc
     4561 atcattccct tcatgcttct catcatcttc gccattccgc ctttcttcat tttcttcatc
     4621 atgcgctgca tgtcgtcgaa ctgcttcaga aggcggttaa cgtcctgtac ctgcatcccg
     4681 caaccggcgg cgatacgacg cttacgcgaa cctttgatga tttctggctt agcgcgctct
     4741 ttcatcgtca ttgagttgat gatggcttcc atacgcacca gcactttatc gtccatctgc
     4801 gacttgacgt tatccgggat ctgccccatg cccggcagtt tgcccatcaa gctggccatg
     4861 ccgcccatat ttttcatctg gcgcagttgc tcaagaaagt cgttgagatc gaagccgtcg
     4921 ccttttttta gcttgctggc taatttctct gcctgcgcgc ggtcaacttt gctttcgata
     4981 tcttcgatca gcgacagtac gtcgcccatg ccgagaatac gcgacgcgat gcggtccgga
     5041 tggaacggct ccagcgcctc agttttctcg ccaacaccga ggaacttgat cggtttgcca
     5101 gtgatgtgac gaatagagag cgccgcaccg ccgcgggcat cgccgtccac tttggtcaac
     5161 actacgccgg taagcggtaa cgcttcattg aatgcttttg ccgtattggc cgcatcctga
     5221 ccggtcatgg cgtcaaccac aaacagggtt tcaaccgggt taatcgacgc atggacttgt
     5281 ttgatctcgt ccatcatcgc ttcgtcaacg tgcagacgac cagcggtatc caccagcagc
     5341 acgtcgtaga atttcagttt ggcttctttc agcgccgcgt taacaatatc aaccggcttc
     5401 tgaccaacat cagaagggaa gaaatcaacg cccacctgct ctgccagcgt ctcaagctgt
     5461 ttgattgccg ccgggcgata gacgtcagca gaaaccacca gcactttctt cttgtgcttc
     5521 tcgcgcagga aattaccgag tttaccaacg ctggttgttt taccggcacc ttgcaggcct
     5581 gccatcagca cgaccgcagg cggttgcgca gccagattca gggtctggtt ctcttcgccc
     5641 atcgccgcaa ccagttcgtt acggacgatt ttgacgaact cctgccccgg cgtcaggctc
     5701 ttattaactt catgaccaac cgctttctct tttacgcgat tgataaactc acgcactacc
     5761 ggcagagcga cgtccgcctc cagcagcgcc atgcgcactt cgcgcagcgt atcttttacg
     5821 ttgtcttcag tgaggcgtcc acggccactg atattgcgca gcgtgcgcga caaacgatcg
     5881 gttaaattat caaacattgt ctctcgcctg gggtggaaac ggttggccgc aatcgcgaca
     5941 catcatcagt attttgccgc agtataacat gaaggcgtct ttgttgttat gcaacggttg
     6001 gagcagcgtt cacctgacgc tatactgctt ctctttctta ttgctcaaac tgtcgacatt
     6061 actatgcccg tttttgctct gctcgcgctt gtcgcctact ccgtcagtct tgcgctgatt
     6121 gttcccggtc tgctgcaaaa aaacggcggc tggcggcgca tggctattat ttctgcggtc
     6181 attgcgctgg tctgccacgc aatcgctctg gaagcccgca tcctgcccga cggtgatagc
     6241 ggacaaaacc tcagcctgct gaacgttggt tcattggtca gtttggtgat ctgtacggta
     6301 atgaccattg tggcttctcg caatcgtggc tggttgctgc tacccattgt ctatgccttt
     6361 gcgcttatca acctggcgct ggcgaccttc atgcccaatg aatacatcac ccatctggaa
     6421 gcaacgcctg ggatgctgat gcacattggc ttatcgctct tttcctatgc cacgctaatt
     6481 atcgccgccc tgtacgcgct gcaactggcg tggattgatt accaactgaa gaacaagaag
     6541 ctggcattta accaagaaat gccgccattg atgagtatcg agcgtaaaat gttccacatc
     6601 acgcagattg gcgtggtgct gctaacgctc acgctttgca ctggcctgtt ctacatgcac
     6661 aacctattta gcatggaaaa tatcgacaag gctgtgctct ctatcgtggc gtggtttgtc
     6721 tatattgtgc tgctgtgggg acattaccat gaaggatggc gtggacgccg cgtcgtctgg
     6781 tttaacgttg cgggcgcggt aattctgaca ctggcctact tcggcagccg aattgtccag
     6841 cagttaatca gctaaaccca gaaaaggagt ttcccctgga acacatttct actactacgt
     6901 tgatcattat tctgatcatc atggtggtca tttcagccta tttttccggg tccgaaaccg
     6961 gaatgatgac cctcaaccgc tatcgtcttc gacatatggc gaaacagggt aatcgctcgg
     7021 ccaaacgcgt cgaaaaattg ctgcgtaagc cagaccgcct gataagcctg gtgttaatcg
     7081 gcaataacct ggtcaatatt cttgcctccg cgctcggcac tattgtcggg atgcgtttgt
     7141 acggcgatgc gggcgtggca attgcgactg gtgtgctgac ttttgtcgta ctggtttttg
     7201 ctgaggtatt gccgaaaacc attgccgcgc tgtacccgga aaaagtcgct tatccgagta
     7261 gttttctgct ggctccgctg caaattttga tgatgccgct ggtctggttg ctgaatgcta
     7321 tcacccgtat gctgatgcgc atgatgggta tcaaaaccga tatcgtggtt agcggctctt
     7381 tgagcaaaga agagttgcgc actatcgtgc atgaatcgcg ctcacaaatt tcccgtcgca
     7441 atcaggatat gctgctgtcg gtgctcgatc tggaaaaaat gaccgttgat gacatcatgg
     7501 taccgcgcag tgaaattatc ggtattgata tcaacgatga ctggaaatct attctgcgcc
     7561 aactctccca ctcacctcac gggcgcatcg tgctttaccg tgattcgctg gacgacgcca
     7621 tcagtatgct gcgagtacgt gaagcctggc ggttgatgtc ggagaaaaaa gagttcacca
     7681 aagaaaccat gctgcgcgcc gcggacgaga tctattttgt gcctgaaggt acgccgctca
     7741 gcacgcagtt ggtaaagttt cagcgcaaca aaaagaaagt cggcctggtc gtcaacgagt
     7801 atggagacat tcaggggctg gtgacggttg aagatattct ggaagagatt gttggcgatt
     7861 tcactacgtc gatgtcgcca acacttgccg aagaggtcac gccgcaaaac gacggttcgg
     7921 tgattatcga tggcaccgcc aacgtgcggg aaatcaacaa agcctttaac tggcatctac
     7981 cggaagatga tgcccgcacg gttaatggcg tcattcttga ggcactggaa gagatccctg
     8041 tcgcaggcac ccgcgtgcgt attggcaagt acgatatcga tattctcgac gtacaggaca
     8101 atatgattaa gcaggtaaaa gtttttcctg tgaaaccgct gcgcgagagt gtggcggagt
     8161 aacgaaaacg gccctgcatt cgcatgcagg gccgtgaatt attacgaaaa cagaaattac
     8221 gcttttgctt tcgctacagt taccatcgcc gcacgaatcg tacgaccatt cagcgtataa
     8281 cccttctgca taatgcccag tacgttacct ggcgcaacgt catcagattc caccattgcg
     8341 atagcctgat gcacattcgg gtccagcggc acgttagttt cggcgatcac ttcaacacca
     8401 aacttacgca caacatccag catcgacttc agcgtcagct caatgccttc aaccatcgca
     8461 gacatatccg ggttagcttt atcagccact tccagcgcac gatccaggct atcaatcacc
     8521 ggcagcaatt cgttgatgaa tttctccagc gcgaatttgt gggctttttc aatatccagt
     8581 tcagtacgac gacgcaggtt ttccatttcg gcttttacgc gcaaaatacc gtcacgttca
     8641 cgggtctggg cttcagccag ctgagcttcg agattcgcaa ttttttcatc gcgcggatcc
     8701 acctgctcag cagaagcttc tggctcaact gcctcaatct cttcgtgctg atccatgata
     8761 atttcttccg gggcttgccc ctcaggcgtt ttctgttctt tactactcat gaatttctcc
     8821 gcgttttttt cgcattcatc tcgctaactt cgcttattat ggggatcagt ttcagggttt
     8881 caagggaagc actcacattg tcatcaatct tcgcaacaag gacctcggaa aaatgaataa
     8941 tcatttcaag tgtattggca ttgtgggaca cccacggcac cctactgcac tgacaacaca
     9001 tgaaatgctc taccgctggc tgtgcacaaa aggttacgag gtcatcgttg agcaacaaat
     9061 cgctcacgaa ctgcaactga agaatgtgaa aactggcacg ctcgcggaga ttgggcaact
     9121 ggctgatctc gcggtagtcg ttggtggcga cggtaatatg ctgggcgcgg cgcgcacgct
     9181 cgcccgttac gatattaaag ttattggaat caaccgtggc aacctgggtt tcctgactga
     9241 ccttgacccc gataacgccc agcaacaatt agccgatgtg ctggaaggtc actacatcag
     9301 cgagaaacgt tttttgctgg aagcgcaagt ctgtcagcaa gattgccaaa aacgcatcag
     9361 caccgcgatt aacgaagtgg tacttcatcc tggcaaagtg gcgcatatga tagagttcga
     9421 agtgtatatc gacgagatct ttgcgttttc tcagcgatct gatgggctaa ttatttcgac
     9481 gccaacaggc tccaccgcct attccctctc tgcgggcggt cctattctga ccccctctct
     9541 ggatgcgatt accctggtgc ccatgttccc gcatacgttg tcagcacgac cactggtcat
     9601 aaacagcagc agcacgatcc gtctgcgttt ttcgcatcgc cgtaacgacc tggaaatcag
     9661 ttgcgacagc cagatagcac tgccgattca ggaaggtgaa gatgtcctga ttcgtcgctg
     9721 tgattaccat ctgaatctga ttcatccgaa agattacagt tatttcaata cattaagcac
     9781 caagctcggc tggtcaaaaa aattattcta attttacgcc agcctcttta ctgtatataa
     9841 aaccagttta tactgtacac aataacagta atggtttttc atacaggaaa acgactatgt
     9901 tggcacaact gaccatcagc aactttgcta tcgttcgtga gcttgagatt gattttcata
     9961 gcggcatgac cgtaataact ggcgagaccg gtgcgggtaa atctattgca atagatgccc
    10021 tcggtctttg tctcggtggt cgcgctgaag ccgacatggt gcgtaccggc gctgctcgcg
    10081 ctgacctgtg cgcccgtttt tctctgaaag atacgccagc ggccctgcgc tggctggaag
    10141 aaaaccagct tgaagacggg catgaatgtt tgcttcgtcg cgtgatcagc agtgatggtc
    10201 gctcccgtgg tttcatcaac ggtacagctg ttcctctgtc acaactgcgc gaactgggtc
    10261 agttgctgat tcagatccat ggtcagcacg ctcatcaatt actcaccaaa cctgagcacc
    10321 aaaaattcct gcttgatggc tatgccaatg aaacctctct acttcaggaa atgaccgcac
    10381 gttatcagtt gtggcatcaa agctgccgtg acctcgcgca tcatcaacag ttaagtcagg
    10441 aacgcgccgc ccgtgcggaa ctgctgcaat accaattaaa agaacttaac gaatttaatc
    10501 cgcagcccgg agagtttgag caaatcgacg aagagtacaa acgtctggcg aacagcggtc
    10561 aattgctgac caccagccag aatgcattgg cattaatggc cgacggtgaa gacgcaaacc
    10621 tgcaaagtca gctttacacg gctaaacaac tggtgagcga attgattggc atggacagca
    10681 aactgtccgg cgtacttgat atgctggaag aagctaccat ccagattgct gaagccagcg
    10741 acgaactgcg ccactactgc gatcgtctgg atctcgatcc caaccgactg tttgaacttg
    10801 aacagcgcat ctcaaaacag atttcgctgg cacgtaaaca tcacgtcagc cctgaggcat
    10861 taccacagta ttaccagtcg ctactggaag aacagcagca actggacgat caggccgact
    10921 cacaagaaac gctcgcgctg gcggtaacga aacatcatca gcaggcactg gaaaccgcgc
    10981 gcgcattaca ccaacaacgc cagcaatatg cagaagaact tgcacagctg atcaccgaca
    11041 gtatgcatgc gctctcaatg ccgcatgggc agtttacgat tgatgtcaaa tatgacgagc
    11101 atcacctggg cgctgacggt gccgatcgta ttgagtttcg ggtaaccacc aacccaggtc
    11161 agccaatgca gcctattgcc aaagtcgcat ccggtggtga attgtcccgc atcgcactgg
    11221 caatccaggt catcacggcg cgtaaaatgg aaaccccggc actgattttt gatgaagtgg
    11281 atgtagggat tagcggtcca acagcggcag ttgtcggcaa actgctgcgt caactcggcg
    11341 aatcaactca ggtgatgtgt gttacccacc tgccacaagt cgcgggatgt ggtcatcaac
    11401 actattttgt cagcaaagaa accgatggtg cgatgacaga aacacatatg caatctctgg
    11461 ataaaaaagc gcggttacaa gagctggcgc gcctgcttgg tggcagtgaa gtcacacgta
    11521 acacactggc gaatgcgaaa gaactgcttg cagcgtaaac ttttttcctg cttcacggtc
    11581 agagtaaaca gcaaaacgcc gtaagaccgg aaagcaaaag gttttaaagt gatgaaaggt
    11641 ctattatcat cggcatatta cagatgagcc acgtactgct cgggcccgaa aaggaatcaa
    11701 atcactatgc gctgtaaaac gctgactgct gcagcagcag tactattgat gttaaccgca
    11761 ggctgttcca ctctggagcg agtggtttac cgtcctgaca tcaaccaggg gaactatctg
    11821 accgctaacg acgtatccaa aatacgtgtt ggcatgacgc aacaacaagt tgcgtacgca
    11881 ttgggtacac cgctgatgtc cgatccattt ggtacgaata cctggttcta tgtcttccgc
    11941 cagcaaccag gtcatgaagg tgtaacgcag caaacgctga cgctgacctt taacagtagc
    12001 ggtgtgttga ccaatattga taacaaacct gcgctgagtg gtaactaata ataaagttgc
    12061 tctcaaagac gttaaaaaag gtgctcaatg agcacctttt ttctgtctgt tatttattcg
    12121 ctgatttttc tgctcgttgc ctgcgaagct ctttcggatc ggcaatgaga gggcgataaa
    12181 tctccacccg atcgccatca tgcacactat cgcttagttt tgccggacgg ctgtaaatgc
    12241 cgactttgtt tttagttaaa tcgatatcgg tacgcaattc cagcaagcca ctggcgcgaa
    12301 tagcttcttc aaccgtcgcg ccctcctgca gcgtcactcg ctgcaggtat tgcttctcag
    12361 gtagcgcata agccacctca acggcaattt tacctagcac tgtaaacctc tttcgcacga
    12421 accgtaaaag cctggaccat attagccgcc agctctttaa acacgcgacc aaaggcgagt
    12481 tcaatcaact tattggtaaa ctcaaagtcg agatgaaact cgatacgaca cgcctcctgg
    12541 ctcagcggcg taaacttcca tccaccaatc aatttcttga acggcccatc caccagattc
    12601 ataagaatac tttggttact ggtcaactgg ttgcgggtag taaacgtttt gctgatccca
    12661 gccttagaga catctaccgc cgcagtcatc tgcccaggag tggactccag aatccgactt
    12721 ccggtacaac ctggcaaaaa ctgaggataa gactgaacgt cattcactaa ctgatacatt
    12781 tgctccgcgc tgtagggtac cagtgcggtc cggctaatct gaggcataac aatttccatc
    12841 aacaaaaatc caacaaataa tatcatttat cctgctgtaa aaaaaacgct atcccggcgc
    12901 tgggtaacat cgggttcatg ctaagataga gccttgtccc ccgcaggatt gatatggggt
    12961 attttcgatt tcagattacc gatgattcac gacgcttatg acgaagaaaa aagcacataa
    13021 acctggttca gcgaccatcg cgcttaacaa gcgcgcccgt cacgaatact ttatcgaaga
    13081 agagttcgaa gcgggacttg ccctgcaagg ctgggaagtt aaatccctgc gcgcaggaaa
    13141 agccaatatc agcgacagct acgtccttct gcgtgacgga gaggcatttc tgtttggcgc
    13201 taacatcacg ccaatggccg tggcctccac gcatgtggtg tgcgatccta cccgtacccg
    13261 caagttactt ctcaaccagc gcgaactgga ctcattgtac ggtcgcgtca atcgagaagg
    13321 ctataccgta gtggcgctct ccctgtactg gaaaaatgcc tggtgcaaag tgaaaatcgg
    13381 cgtcgccaaa ggtaaaaaac agcacgataa acgttcagat atcaaagaac gcgaatggca
    13441 ggtggataaa gcacgcatca tgaaaaacgc ccaccgttaa acctgcactc caattattga
    13501 ccagttcctc accgcgcctc cctctccggc ggcgcgaatg aacatcttat tggctatcac
    13561 atccgacaca aatgttgcca tcccattgct taatcgaata aaaatcaggc tacatgggtg
    13621 ctaaatcttt aacgataacg ccattgaggc tggtcatggc gctcataaat ctggtatact
    13681 tacctttaca cattggggct gattctggat tcgacgggat ttgcgaaacc caaggtgcat
    13741 gccgaggggc ggttggcctc gtaaaaagcc gcaaaaaata gtcgcaaacg acgaaaacta
    13801 cgctttagca gcttaataac ctgcttagag ccctctctcc ctagcctccg ctcttaggac
    13861 ggggatcaag agaggtcaaa cccaaaagag atcgcgtgga agccctgcct ggggttgaag
    13921 cgttaaaact taatcaggct agtttgttag tggcgtgtcc gtccgcagct ggcaagcgaa
    13981 tgtaaagact gactaagcat gtagtaccga ggatgtagga atttcggacg cgggttcaac
    14041 tcccgccagc tccaccaaaa ttctccatcg gtgattacca gagtcatccg atgaagtcct
    14101 aagagcccgc acggcgcaag ccctgcgggc ttttttgtgc tccgaatttg tcccgcgaag
    14161 tctgatgcca actaattaaa tccgatcctt ttaggcacct tgttaggcac ttcataaagc
    14221 tatattgtct ttgaggtgcc taaaactatg gaaacccggc aatggcaaga caaaccaaac
    14281 ctctatccgt taaggaaatc gaatctgcta aacccaagga agcggactac gttctctatg
    14341 atggcgatgg ccttgagcta ctcattaaat ccagcgggag taaaatctgg cagtttcgct
    14401 acattcgccc tgtcaccaag acacgagcaa agaaaagcat aggcccttac ccgtcagtta
    14461 cccttgccga tgccagaaac taccgtgcag agtcccgctc actcctagct aaacagattg
    14521 atccgcagga acatcagcaa gaacaattgc gtagttcgct ggaagccaaa accaatactt
    14581 tccagctcgt agctgaacgt tggtggaatg tgaagaaagc cagtgtgaca gaggactatg
    14641 cggaagatat ctggcgctct cttgaaagag atgtctttcc tgcgattggc gacgttagcg
    14701 ttacagatat taaagctcat acactggttc aggccgtaca accggttcag gccagaggag
    14761 cactggaaac ggttcgtcgc ctgtgtcaac gtattaatga ggtcatgatc tatgcccaga
    14821 acacagggct gattgatgct gttcccagcg ttaatatcgg taaagccttc gaaaagcctc
    14881 agaaaaagaa catgcccagc attcgaccgg atcagctatc tcaactgatg cagacaatgc
    14941 gaacagccag cattagcctt tccacacgct gcctgttcat gtggcaactt cttactatta
    15001 cccgccctgc cgaagcggct gaagctcgct gggaagaagt agacatagaa gcgcaagagt
    15061 ggaagattcc cgcagcacgc atgaaaatga accgcgacca tactgttcca ttgtcagatg
    15121 aagcaatagc tgtgctggag atgatgaaac cgctaagcgg caaccgagaa tttatctttc
    15181 ctagccgtat caagcccaac cagcttatga acagtcagac tgttaacgcc tcactaaaac
    15241 gcgcaggttt tggtggtgtg cttgtttcgc atggtctgag atcaattgcc agtacggctc
    15301 ttaatgaaca aggctttccg cctgatgcca ttgaggcagc attggcccat gtggataaga
    15361 atgaggttcg tcgtgcttat aaccgcagcg attacctgga acagcgtcgc ccgatgatgc
    15421 aatggtgggc aaattttgtg atggcagcgg atcgtggaag catgattgaa ggtgggataa
    15481 aaggaatgaa gctagttggg tgactaaata gctgcgcggc atagtgtccc actttcagcc
    15541 agcccttgca ggttaagcca ccagacagta aacggcgggt gtcgcgttat taggcgcagc
    15601 tatttagtag taatattagg attggtgatt ctgcactcca gaagtt
//
LOCUS       DAZXUK010000078        15583 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZXUK010000078 DAZXUK010000000
VERSION     DAZXUK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15583
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.078"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            129..671
                     /locus_tag="ACH3L8_001927"
     CDS             129..671
                     /locus_tag="ACH3L8_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFT4085061.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            758..1960
                     /locus_tag="ACH3L8_001928"
     CDS             758..1960
                     /locus_tag="ACH3L8_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFT4085062.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2024..2485
                     /locus_tag="ACH3L8_001929"
                     /pseudo
     CDS             2024..2485
                     /locus_tag="ACH3L8_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(2526..3476)
                     /locus_tag="ACH3L8_001930"
     CDS             complement(2526..3476)
                     /locus_tag="ACH3L8_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFT4085063.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3669..4859)
                     /gene="glpC"
                     /locus_tag="ACH3L8_001931"
     CDS             complement(3669..4859)
                     /gene="glpC"
                     /locus_tag="ACH3L8_001931"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFT4085064.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4856..6115)
                     /gene="glpB"
                     /locus_tag="ACH3L8_001932"
     CDS             complement(4856..6115)
                     /gene="glpB"
                     /locus_tag="ACH3L8_001932"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFT4085065.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(6105..7733)
                     /gene="glpA"
                     /locus_tag="ACH3L8_001933"
     CDS             complement(6105..7733)
                     /gene="glpA"
                     /locus_tag="ACH3L8_001933"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFT4085066.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            8006..9364
                     /gene="glpT"
                     /locus_tag="ACH3L8_001934"
     CDS             8006..9364
                     /gene="glpT"
                     /locus_tag="ACH3L8_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFT4085067.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9369..10445
                     /gene="glpQ"
                     /locus_tag="ACH3L8_001935"
     CDS             9369..10445
                     /gene="glpQ"
                     /locus_tag="ACH3L8_001935"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFT4085068.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(10487..10729)
                     /locus_tag="ACH3L8_001936"
                     /pseudo
     CDS             complement(10487..10729)
                     /locus_tag="ACH3L8_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10724..10828
                     /locus_tag="ACH3L8_001937"
                     /pseudo
     CDS             10724..10828
                     /locus_tag="ACH3L8_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10908..11558
                     /gene="inaA"
                     /locus_tag="ACH3L8_001938"
     CDS             10908..11558
                     /gene="inaA"
                     /locus_tag="ACH3L8_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFT4085069.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(11612..11866)
                     /gene="yfaE"
                     /locus_tag="ACH3L8_001939"
     CDS             complement(11612..11866)
                     /gene="yfaE"
                     /locus_tag="ACH3L8_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFT4085070.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(11866..12996)
                     /gene="nrdB"
                     /locus_tag="ACH3L8_001940"
     CDS             complement(11866..12996)
                     /gene="nrdB"
                     /locus_tag="ACH3L8_001940"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFT4085071.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(13230..15515)
                     /gene="nrdA"
                     /locus_tag="ACH3L8_001941"
     CDS             complement(13230..15515)
                     /gene="nrdA"
                     /locus_tag="ACH3L8_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFT4085072.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         3590 a         3884 c         4257 g         3852 t
ORIGIN      
        1 ttatttaaga agtatttagt tacaataagt gactagatcc cttttttcgc tcattgaatc
       61 ctctgtgcca ggcagtacaa tcccgcagct ttgggaattt tgattaattg cttgtgggaa
      121 aggggataat gaaaaaaatt gcgctagcag gtctggccgg aatgctgttg gtttctgcat
      181 cggtcaatgc aatgagcatc agcggccagg cgggtaaaga atacaccaat attggtgtcg
      241 gttttgggac tgaatcgacg ggcctggctt taagcggtaa ctggacacat aacgacgacg
      301 acggtgacgt cgcgggcgtg gggctggggt tgaatctgcc tctcgggccg ttaatggcga
      361 ccgttggcgg aaaaggcgtg tacaccaacc cgaattacgg cgatgaaggt tatgccgcag
      421 cggtaggagg tggtttgcag tggaaaattg gcaacagctt ccgtttgttt ggcgagtatt
      481 actactctcc ggattcgctc tccagcggta ttcaaagtta tgaggaagcg aatgctggcg
      541 cgcgttacac cattatgcgt ccagtcagta ttgaggcggg ttatcgctac ctgaatctgt
      601 cgggtaaaga cggtaaccgc gacaacgctg tggctgacgg cctgtatgtt ggggttaacg
      661 ccagtttctg atcccttccg gcgcggcatt cagtttctta ctacgcctta tctccctttg
      721 cgttatagtg attcgataac aaaagcagga gataaccatg ttaaaagtgg aaatgctatc
      781 caccggggat gaagtgttat acgggcaaat cgttgacact aacgctgcct ggctggccga
      841 ttttttcttt catcaggggc tgccattatc tcgccgcaat acggtggggg ataaccttga
      901 tgacttagtc accattcttc gcgaacgtag tcagcacgcc gatgtgctga tcgttaacgg
      961 cgggctggga ccgaccagcg atgatttaag cgcactcgcc gctgcgacag caaaaggtga
     1021 aggcctggtg ctgcatgaag cctggctcaa agagatggaa cgctatttcc acgaacgtgg
     1081 acgagtaatg gcaccgagca accgtaaaca agcggagctg cctgccagtg ctgaatttat
     1141 caataacccg gtaggcaccg cctgtggttt tgccgtgcag cttaatcgtt gcctgatgtt
     1201 ctttactccc ggcgtaccgt cagaatttaa ggtgatggtc gagcacgaaa tcctgccgcg
     1261 cctgcgcgag cgtttttctt taccacagcc gccggtttgt ctgcgtttaa ctacctttgg
     1321 tcgttcggaa agcgatctgg cacaaagcct ggacactcta caactgccgc cgggcgtaac
     1381 aatgggctat cgctcctcaa tgccgatcat cgaactgaaa ctcaccggac cggcaagcga
     1441 gcaacaggcg atggaaaaac tgtggctgga cgttaaacgt gttgccggac agagcgtgat
     1501 tttcgaaggc accgaaggac tgcccgcgca gatcagtcgc gaattgcaaa accgccagtt
     1561 cagcctgacg ttgagcgagc aattcaccgg tggtttattg gctttgcaac tttctcgcgc
     1621 aggtgctcca ttgctggcgt gtgaagtgat tccttcacag gaagaaaccc tggcgcaaac
     1681 tgcgcactgg attacagaac ggcgggccaa ccattttgcc gggctggcac tggctgtttc
     1741 gggtttcgag aacgagcatc tcaactttgc gctagccacg ccagacggca ctttcgctct
     1801 gcgtgtgcgt ttcagcacta cacgctacag cctggctatc cgtcaggaag tgtgcgcaat
     1861 gatggcactg aatatgctac gccgttggtt aaacggccag gacatcgcta gtgagcatgg
     1921 ctggattgag gttgttgagt ccatgacttt atccgtctga tttcgccact ctgtgccggt
     1981 ttactgcacc ggcacattcc ccctccggca aaatttgtcc aaccctccct acggtgagcg
     2041 tggtgttggg gccagtgtgg cacgggctgc gcgctgggga cgcattgaga attacatggc
     2101 gcaagtcaac gattcgcttt gtctgttagt gcaggtggaa agtaaaacgg cactggataa
     2161 cctggacgag atcctcgacg tcgaagggat tgatggcgtg tttattggac cagcggatct
     2221 ttctgcgtcg ttgggctacc cggataacgc cgggcacccg gaagtgcagc gaattattga
     2281 aaccagcatt cggcggatcc gtgctgcggg taaagcggct ggttttctgg ctgtggctcc
     2341 tgatatggcg cagcaatgcc tggcgtgggg agcgaacttt gtcgctgttg gcgttgacac
     2401 gatgctctac agcgatgccc tggatcaacg actggcgatg tttaaatcag gcaaaaatgg
     2461 gccacgcata aaaggtagtt attgatatca aaggcccatg gggatcggct gtgggcctgt
     2521 gttaattagt ggttattcgc tgccagatcg gcttcgctta gctgggtggc cgcgagcact
     2581 tggtcacgat caatgccctg ttccagcatg cgcaaagcaa ttttaatggc ttgttcatgc
     2641 ataccttcct gcaaaccttc taatttacct tgctgcaaac ctactaattt accttcttgc
     2701 cagccaattt gatgtccttc ctgccgtagc cgttcagcaa tagtcattaa tctctccttt
     2761 tgtagtggtg aacgttcggc aatctcctga ataaaacggg tgaaacggga ggtatcgccg
     2821 cattgcagca gataattaaa cagtgtttgt agctggctgt cattagtgaa gcctctaacc
     2881 aaaagcgtgg tgatcctgtc gaccatgccg attaaatcgt ggtcgcgaat atgcttttga
     2941 atcagttcca gcagagctat acgccgatgt tgcatgatct catcgtcagg tacgatggtg
     3001 atatccacca acggaaaagc ttcggtgtac aactgccggg caagttgcgg atcgtcaaac
     3061 tcatccagcc agttaagtga gtacgggtag ggcgatgttt cgccatgata aaacagcaat
     3121 ggcaccacca gcggaactct gtcatagcct ttatctaggt gactctgcat ggcggcagtg
     3181 gcatagcgca ttggccgaaa agccatattc ttttctgcag agctttgatg ttcaatcacg
     3241 cagtagatat agccgtcacc gtcgctggtt tccacggacc acaaaacatc cgagtagtaa
     3301 gcgcgtaaac ttttttcaat aaaactagtg ggttccaggc gtaaggtttg caggttgcaa
     3361 agcttgcgca gtggttctgg taaatgtatt tcgagaaaat cccgtgcggt ttcgggtgtg
     3421 aatataaagg ttttaaatac cgcatcatgc ggggaggagg ttgttgattc ggtcatggtt
     3481 gtccgtcacc tgatatttat gatgacgcga cattacggtt atcccaggcc tttgacatct
     3541 gccagtttca gcctttgcga gacgttttca gaggaatttt cactgcgttg caacgcatac
     3601 attgggcacg gaatcgtgta cgcaaaaatg cccggtaacc acatgcttac cgggcatcag
     3661 aagggagttt aagccagcgc ctgggccagt agcgtaatcg gatgttcgca gcgaagactt
     3721 gtggacatct caatctgcca tttacaggtt tcgcagtcgg tgaccaccag atctgcgccg
     3781 ctttcttcta tctggcggaa cagtggtgcg ccgatggctt gtgaggtggg gtagttctct
     3841 tttttgaaac cgtaagtacc cgcaataccg cagcactggg aatccagcac cgttaactca
     3901 agccccggga tgttacgcaa cagctccagg gtgtagagcg tccagcccat tttttccata
     3961 tggcacggag tgtgataaac cactttcagc ggcagcggtt tcagcggtaa cgttttgcct
     4021 tcgtccagct tgcgccacag ccagcgggtt gccagttcga tatgatcgcg caagcctttg
     4081 ttgtcgacat tcagcacttc cgggtattcg tcgcgcaggg caaatgtaca ggttgaggag
     4141 gtggcaatca ccggaatgcc ttttactccc acagcttcgc ggatcgactc tacattcgta
     4201 attgcctgtt tgcgtgcttt atcggtaaag ccgttggcga ttagcggtac gccgcagcat
     4261 ttttctttgc tgagcagttg tacaccggta cccattgcgt tgagcacttt aattaaatct
     4321 ttacctaact gcggatggtt gtagttaacg aagcagccgt gaaagaaagc gacctggtct
     4381 ttatattgtg cttgctgagc cgctatgctg cgataccagc gacggaacgt gccgaaggag
     4441 tatttcggta gcgtgcgacg atgatcgatt tttaacgccg catcaagcag ctgccgcacc
     4501 ggtttcagcg aggtagcggt gttgacgatt ggtgcgaacg gcgtggaaac gctacccatc
     4561 aggtcggtat gactcaacac aaaattacgc agcgacgggc gcgtggtgtc atatttcgcc
     4621 cgcgcgcgct ggataatatc gccaatcttc acatcggacg ggcaggcgac ttcacaacgt
     4681 ttgcagttga tgcaatattt cagcgcctcg tcatacagtg cgccatcttt caaacgcaga
     4741 cgctcgccat ccggcccggc ttgttttggc cctggataac cgggattcac ccggctcacc
     4801 gggcaggcgg tggtgcagac ggtgcactta atgcagtttt cgaagctggt gtcgttcatt
     4861 gttggcctcc tgcgcgttgg gcaatctgtt gagcggcatg taaagcactg acggcacaaa
     4921 caccgccgcc gcatccctgg gcgatgggat caaatccgcc cagcaccgaa ccgatggcaa
     4981 acaggttctc aatggtttgc cctgcctgcg acgggcgtag cgtctcatca gtggttacac
     5041 cgaactgctg ccacggttgc ggcgcaaaaa aatcgccctt ataccattca ccccgcgtgg
     5101 cggtttgtag cacatcaagg ccgagaatcg gctcgcgaat gccgttacgt tccgctacta
     5161 gtccgccact aaagaaactg ccgctggcga gaaccgcgaa acgtggacgt agcggaatat
     5221 cggcgtgatt gcgggtccag atttcattca ctacgccatt tttacaggtc acttttttca
     5281 cttcatcgcc cggcatccac actccacctt ggcgcacaaa ctggcgctgt aactggtttt
     5341 gcagacgaat gcccagcacg gaaggcggca gcgttggcaa aagcatcagt gaacaaggta
     5401 gtttttcatt caaccaacgc cacagtttgt catcggccag gccgaagcag gcgggcatca
     5461 ggatcatttc gcaggtattg gcgacaggaa taagcgcatc aagtaacagc ggccagtttt
     5521 cttcattatc aaggaaacgg gcgatattca ccgcgcgaaa ttcggtggcg ttattgcgca
     5581 ggacatccag ttccggcagc tctatttctg cggtttcaac cgaaagatcc agttcgcgca
     5641 acgaagccgc cgcaagatgc gcctggaaat ccatcagccc gcttatcccc actacacata
     5701 tcttcttcgc gggtagcggc cagacgggaa cttctggcga acttaaccag gttgcacgca
     5761 aagtgccgag cggcgtaatc cgctggtgag ccagttctac gctgccctgc aattgcgcac
     5821 cgctttcagc gattaatgcc tgcgcctggc aagcgagatc gagcacgcgt tgtggcccga
     5881 gaagagagta aggatgtgca ggtgcctgct gacgcaaaga ttccagtcca ctgtgaatgt
     5941 ctgccacggg ttgaccatct ggcagatggc tcagcaaatc cagcgaaccg gatgagaaat
     6001 gcaacgcgct ttgaccacgc gtgacaatgg cacagcgcag gccgtgtttt tgcagttgca
     6061 ggccacagag taatccggcg aggccgccgc ccataatgac agtatcaaag cgcatctttc
     6121 tgctccttct ccagaccaca cagtccctga taaacccagc gggtaaattc gctttcgcgc
     6181 agtgcgtctc cccaggcgat gggttgcacg cctttccagc gttcgttaag gaaggtggaa
     6241 agttgctcga tagattgcgc ggacgtcgtg acgttaaacc gttgcagcag tccggcagcg
     6301 cggcaggcgc agagttcgcc ctggcaggtg cccatcccca cacgggtacg acggcgtaaa
     6361 tccagcaggc tattaacgtt taaattttct accgcgtact gcacttcacc cgcagttacc
     6421 gcttcgcact cacataccag gctacggtgt agacggcctt cgctcagcca ggcaggcgtg
     6481 cgatcgccat gacgataaac cgcagaaccg cgcagcgggg caggcaggga gatgacttta
     6541 cgcaaggtaa cttcagcggg ttcttgtgaa ccaggcagtg ccagatcggc agtcgtacag
     6601 gggcgcgtgt tgcccagttt gcggcatacc gcgtcggtag cccattcagc catcagccga
     6661 taggtcatca gtttgccacc ggtgatggtg ataaatccgt ccagaccatc gcgttcagca
     6721 tggtcgagca gcacgatgcc acggctgacg ttacgtccgc tcgggtcgtc atcgctggca
     6781 accagcgggc gcacgccaga ataggcccgc aaaatgcgcg ttttcgccat cacgggggcc
     6841 agtttttccc cttcacgcag cagaatatca acctcttctg ccgtcactcg attatcgtca
     6901 atctcgttgt aatcaatacg taaagaggtg gtaccaatca gcgaaatggt atcgccaggc
     6961 accagaatat cggcgtcgga aggtttacgg cagcggttga tcacatgctg gttaatgcgg
     7021 tgatccatga tcagcagcga tcctttcgcc gggaacatgc gaatgcgcag atcggcatat
     7081 tcggcaatgt gttgccccca gatcccagcg gcattaacca cgacaggtgc atgaagggcc
     7141 tgagtttcgc cggtgagatg gttacgtaca cgaacaccgc acaccgtcgc gccttcacga
     7201 atcagccccg tgacttcatg agcggtaagg ataacggcac cgtgttcttt ggcatccagc
     7261 atgtttgctg cggtcagacg aaatggatca acggtgccat ccggaacttt caccgcgcca
     7321 atcagtgccg ggttaacggc aggttcgata atgcgcgctt gctgcgggtc tatagcttct
     7381 gcgctgatcc ctgcttcttc gcaggcgcga ataaaagtgg cctggaatga gaggtcatct
     7441 tccggcaggg tgataaacag gccgttggtt ggttcaacgc agtgacgtgc aatgcgtttc
     7501 aggatctggt tttcactaat gcattcgcgg gccgattccg catcggttac tgcatagcgc
     7561 gcaccgctgt gcagcaggcc gtggttacgc ccggtggcac cggttgcgat gtcgtggcgc
     7621 tcaaccaaaa tcacgcgcag cccgcgcagg gcacagtcgc gggcaatccc ggctcccgtt
     7681 gcgccgccgc caatgataat cacgtcactt gattgcgagt cgcgagtttt cattgttatc
     7741 cctctgaagt tcgtttttta ccatttagcc atagtaaaaa catgaattgt ttgatttcgc
     7801 gcatattcgc tcatgattcg aaagtaaaac gtgatttcat gcgtcatttt gaacattttg
     7861 taaatcttat ttaataatgt gtgcggcaat tcacatttaa tttatgaatg ttttcttaac
     7921 atcgcggcaa ctcaagaaac ggcaggttct ctcactgaat caggctgtta atcataaata
     7981 agaccacggg ccacggaggc tatcaatgtt gagtattttt aaaccagcgc cacacaaagc
     8041 gcgcttacct gccgcggaga tcgatccgac ttatcgtcga ttgcgctggc aaattttcct
     8101 ggggatattc tttggctatg cggcttacta tttggttcgt aagaactttg cgcttgctat
     8161 gccttatctg gttgagcagg gattctcacg cggtgattta ggttttgccc tttcggggat
     8221 ctcgattgct tatggatttt cgaaattcat catgggttcg gtatcggatc gctcgaatcc
     8281 gcgcgttttc ctgcccgcag gtttgattct ggcggcggca gtgatgttgt ttatgggctt
     8341 tgtgccatgg gcgacgtcga gcattgcggt gatgtttgta ctgttgttcc tctgcggttg
     8401 gttccagggg atggggtggc cgccgtgtgg tcgtactatg gtgcactggt ggtcgcagaa
     8461 agaacgtggc ggcattgtgt cagtgtggaa ctgtgcgcac aacgtcggtg gtggtattcc
     8521 gccgctgctg ttcctgctgg ggatggcctg gttcaatgac tggcatgcgg cgctctatat
     8581 gcctgctttc tgcgccattc tggtggcatt attcgccttt gcgatgatgc gcgatacccc
     8641 gcaatcctgt ggcttgccgc cgatcgaaga gtacaaaaat gattatccgg acgactataa
     8701 cgaaaaagcg gaacaggagc tgaccgcgaa gcaaatcttc atgcagtacg tgctgccgaa
     8761 caaactgctg tggtacatcg ccatcgccaa cgtgttcgtt tatctgctgc gttacggcat
     8821 cctcgactgg tcaccgactt atctgaaaga ggttaagcat ttcgcgctgg ataaatcctc
     8881 ctgggcctac ttcttctatg agtatgcggg tattccgggc accctgctgt gcggctggat
     8941 gtcggacaaa gtcttccgtg gtaaccgtgg ggcaaccggc gtcttcttta tgacactggt
     9001 gaccatcgcg actatcgttt actggatgaa cccggcaggt aacccaaccg tcgatatgat
     9061 ttgtatgatt gttatcggct tcctgatcta cggtcctgtg atgctgatcg gtctgcatgc
     9121 gctggaactg gcaccgaaaa aagcggcagg tacggcagcg ggctttaccg ggctgtttgg
     9181 ttacctgggc ggttcggtgg cggcgagcgc gattgttggc tacaccgtgg acttcttcgg
     9241 ctgggatggc ggctttatgg taatgattgg cggcagcatt ctggcggtta tcttgttgat
     9301 tgttgtgatg attggcgaaa aacgtcgcca tgaacaatta ctgcaaaaac gcaacggagg
     9361 ctaatggcat gaaattgaag ctgaaaaacc ttagcatggc gatcatgatg agcactatag
     9421 tcatgggaag cagtgcaatg gcggcggaca gcaacgaaaa aatagtcatc gcccatcgcg
     9481 gtgccagtgg atatttgccg gagcatacgc tgccagcaaa agcgatggcg tatgcgcagg
     9541 gagcggatta tctggaacag gatttggtga tgaccaaaga cgaccatctg gttgttctgc
     9601 atgaccatta tctcgatcgt gttactgatg ttgccgatcg tttcccggat cgggcgcgca
     9661 aagacggtcg ttactacgcg atagatttca cgctggatga aattaagtcg ctgaaattta
     9721 ccgaaggttt cgatattgaa aacggtaaaa aagtacagac ttatccgggg cgtttcccaa
     9781 tgggtaagtc cgacttccgg gtgcacacct ttgaagaaga gattgaattt gttcaggggt
     9841 taaatcactc taccgggaaa aatatcggta tctatccaga aatcaaagcg ccgtggttcc
     9901 atcatcagga agggaaggat attgcggcaa aaacgctgga agtgctgaag aaatatggtt
     9961 acaccggtaa agacgataaa gtttatttgc aatgttttga tgctgatgag ctgaagcgta
    10021 ttaagaatga gctggaaccc aaaatgggca tggagctcaa tctggtacag ctgattgcct
    10081 ataccgactg gaatgaaacg cagcagaaac agccggatgg aagctgggtt aattacaact
    10141 acgactggat gtttaagccg ggagccatga aacaggtggc ggaatatgca gatggtattg
    10201 gtccggatta ccatatgttg attgaggaga catcgcagcc gggtaatatc aaactcactg
    10261 gcatggtgca agatgcccag cagaataaac tggtagtgca tccttatacc gtgcggtcag
    10321 ataaactgcc tgaatacact cctgatgtga atcagttata tgatgctctg tataacaaag
    10381 cgggtgtaaa tgggctgttt actgatttcc ctgataaggc agtaaaattt cttaataaag
    10441 agtaatgtta ctgcaggatg gcgttcatgc gccatcctgt ctcatatcaa atttttccaa
    10501 tcgccactaa ttgttccatg cacatttgta gtaaaaacag tggactgcct tctaccggct
    10561 tttctttagt cagcaacgaa atctgtcgat agaaggttgg ttcgtgagga acggaacaca
    10621 attcacctgc aatgcttttc agcgttaact ctggttgtaa ggcgatgcct aatccctgtc
    10681 gaataaaatt catcgctgta tccgactgat taaattcaca ttttatctgc tgggcgcact
    10741 attagtggta gtggctggtg gaatctatgc gctgcaaatg aagggcaaaa gcggtgtcac
    10801 acctgtagcg gcaaaagaaa ttcattaata cgacacgttt cattaagatt ttcctcaggt
    10861 aaccacctat agtcattgtc gttaataaaa atgacaggcg atagggtatg gcagtttcag
    10921 caaagtacga cgaatttaat cactggtggg caacagaggg cgactgggtt gaagagccta
    10981 actatcgccg taatggtatg agcggggtgc aatgcgtcga gcgcaacggc aaaaagctgt
    11041 atgtaaagcg catgacgcat catctgtttc attcagtacg ttatccgttc ggccgaccaa
    11101 cgattgtccg tgaggttgcg gtaattaaag aactggaacg ggccggtgtc atcgtgccga
    11161 agatcgtttt tggtgaagcg gtgaaaattg agggtgaatg gcgcgcgttg ctggtgactg
    11221 aagatatggc ggggttcatc agcattgctg actggtatgc ccggcatgca gtatcgcctt
    11281 attctgacga agtacggcaa gccatgttga aagcagtggc actggcgttt aagaaaatgc
    11341 atagcgttaa tcgtcagcat ggctgttgct atgttcgcca tatctatgtg aaaacagaag
    11401 gtaaggcaga agctggtttt cttgatctgg agaaaagtcg tcgccgcttg cgtcgcgata
    11461 aagcgattaa tcatgacttc cgccagctgg aaaaatatct ggagccgatc cctaaagcgg
    11521 actgggagca ggtgaaagcg tattactacg ccatgtaatc gttgtaggcc agataatacg
    11581 cgtaagcgtc gtatctggcg ctagtacccg atcacaactc gatttcaata tcgccttttg
    11641 cccggcaaca acagggcaaa atttcccccg gctgaataaa ggctaacggt tcggcaatcc
    11701 agtccacctg acctgcgacc agtcgggtgc gacaggagcc gcagtaacct tcgcggcact
    11761 ggtactcaac cgccacattg tgggattcca gcgccgccag aagggaaggg tgttcatcct
    11821 ggcacagcag ttgtgtgcca gtgatgcgca gggtaacgcg ggccatcaga gctggaagtt
    11881 actcaaatcg tcggtgtcca cttccgcgtc aatctgcccg accagataag aactgacttc
    11941 cacttcctgc ggagcaacct gcacgttatc agacaccagc caggtgttga tccacgggat
    12001 cgggttggag cgcgtctgga acggcagatc caaaccgact gcctgcatgc ggatattggt
    12061 gatgtattca acgtactggc agagaatatc tttattcaga ccaatcatcg aaccgtcacg
    12121 gaacagataa tccgcccagt ctttctcttg ctgtgccgcc tggacaaaca ggtcatagca
    12181 ctcctgctta cattcttcgg caatttccgc catctccgga tcatccgcgc cgctgcgcag
    12241 cagattcagc atatgctgag taccggtcag gtgcagggct tcatcgcggg caatcaggcg
    12301 aataatcttg gcgttacctt ccatcagttc gcgttctgca aaggcaaagg aacaagcaaa
    12361 gctgacgtag aagcggatcg cttccagcgc gttaacgctc atcaggcaga gatacagttt
    12421 tttcttcagc tcgcgcaggc taacggttac ggttttaccg ttaacggtgt gggtgccttc
    12481 gcccagaagg tgccagtagc tggtcatttc gatcagttca tcgtaatagc tggagatccc
    12541 ttccgcacgt ttctggatct gctcgttggt gacgatatcg tcaaacacaa cagacggatc
    12601 gttaacgata ttacgaatga tatgagtata ggaacgggaa tgaatcgttt ctgagaacgc
    12661 ccaggtttcg acccaggttt ccagttccgg aatagaaata agcggcaata gcgccacgtt
    12721 cgggctacga ccctgaatgg aatccagcag cgtctgatat ttcaggttgc tgataaagat
    12781 gtgtttttcg tgctccggca gcgcctggta atctatacgg tcgcgggaga cgtcaacttc
    12841 ttccggacgc cagaagaaag agagctgctt ttcgatcagc ttttcgaaga tgtcatattt
    12901 ttgctgatcg tagcgagcca cgttgaccgg ctgaccaaag aacatcggtt ctttgagctg
    12961 atcatttttc gtctgtgaaa aggtggtata tgccatgagt gtgtcctgtt gggagtttaa
    13021 tgccggataa ggcattttac gccgcatcct gtgcctgatg cgacgctggc gcgtcttatc
    13081 aggcctacaa atccgagccg taggccggat aaggcgttca cgctgcatcc ggcacccgga
    13141 gcctgatgcg acgctggcgc gtcttatcag gcctacaaac cgagccgtag gccggataag
    13201 gcgtttacgc cgcatccggc atctcaatat cagatcttac atgcgccgct ttcgcagcca
    13261 tcgtcctgga ttgacggtac cagatcgtcc tgcgcgtctt cagcgccgtc acgggtgttc
    13321 tgataataca gcgttttgac cccgaatttg taggcggtga gcaggtcttt cagcaactgc
    13381 tgcatcggca cttttcctga cgggaaacgt gacggatcgt agttggtgtt agcagagatc
    13441 gactgatcga taaatttctg catgatacct accagttgca ggtagccatc gttacccggc
    13501 atttcccaca gcagctcata ggcgtcgtgc aggtgctcgt agtccggcac cacctggcgc
    13561 aaaataccgt ctttcgacgc tttgatgctg acgtaaccgc gcggcggttc aataccgtta
    13621 gtggcgttag agatctgcga agaggtctcg gacggcatca gggcagaaag cgtggagtta
    13681 cgcaggccgt gcgttttgat tgattcacgc aatgcttccc agtcgtaatg cagcggctca
    13741 ttagcgatgg catccagatc tttcttatag gtatcgatcg gcaggatccc tttcgcgtaa
    13801 gtggtttcgt taaaccacgg gcacgcgcct tgctctttag ccagctcatt agacgctttc
    13861 agcaggtaat actgaatcgc ttcgaaggtt ttatgcgtca ggttgttggc gctgccgtcg
    13921 gagtagcgtt taccgtgctt cgccagatag taagcgaagt taatcacacc aatacccagc
    13981 gtacgacgcc ccatcgctcc acgtttggcg gccgggatcg ggtaatcctg ataatccagt
    14041 agcgcgtcaa gtgcacgaac cgccagaatc gccagctctt ccagttcatc caggctatta
    14101 attgcgccca ggttgaaagc agacagcgta cacagcgcga tttcaccgtt ctcgtcgttg
    14161 acgtcgttca gcggtttggt cggcagggca atttctaggc acaggttaga ctggcgaact
    14221 ggcgcgatgg tcggatcaaa cggactatgg gtattacagt ggtcaacgtt ctgaatatag
    14281 atacgaccgg tagacgcacg ttcctgcatc atcagcgaga acagctcaac ggctttcaca
    14341 cgctgcttgc ggatgctgtc gtctttctca tatttggtat acaggcgttc aaactcttcc
    14401 tgatcggcga agaacgcgtc gtacagcccc ggtacgtcgg acgggctgaa cagggtgata
    14461 tcttcacctt tcagcaggcg ggtatacatc agtttgttga tttgtacccc gtagtccata
    14521 tgacgcacgc ggttgccttc aaccccacgg ttgtttttca acaccagcag gctttccact
    14581 tccagatgcc acatcgggta gaacagcgtt gccgcaccgc cgcgcacacc gccctgagag
    14641 caggatttca ccgctgtctg gaaatgtttg tagaacggga tacagccggt atggaacgct
    14701 tcgccaccgc gaatcgggct gcccagcgca cgaatacgtc cggcgttgat gccgatcccg
    14761 gcacgctggg aaacatattt cacaatcgcg ctggaggtgg cgttgatgga atccaggctg
    14821 tcaccgcact cgatcagtac gcaggagctg aactgacgag tcggggtacg cacgccggac
    14881 atgattggcg tcggcagcga aattttaaat gtggaaaccg cgtcataaaa acgcttcacg
    14941 tattgcaggc gcgtttcacg cgggtagttc gagaacaggc acgcggcaac aagaatataa
    15001 aggaactggg cgctttcgta gatttcgccg gtcacgcggt tctgtactag atatttgcct
    15061 tccagctgct taacggcagc ataagagaag gtcatatcac ggtcgtgatc gataaacgtg
    15121 tccatctgct tgaactcttc ttccgtgtag tcttccagca gatgattatc gtatttgccc
    15181 atctcgacca ttttcaccac gtggtcgtac agcgcaggcg gctcaaactg gccgtaggct
    15241 tttttacgca ggtggaagat cgccaggcgc gcggcgagat actgataatc cggcgcatca
    15301 cgggagatca ggtctgcggc agccttgata atagtttcgt ggatgtcaga ggtcttgata
    15361 ccgtcataaa actgaatgtg ggagcgcagc tcgacctggg aaatcgaaac gttatgcagt
    15421 ccttctgccg cccaatccag aacgcgatgg attttgtcga gattgatgcg ctctgtgcta
    15481 ccgtcgcgct ttgtcaccag cagattctga ttcatgtatg tcgtacctgt ttttggaatt
    15541 ctttccgcaa tagtttcatg cagactacct gtagtaaggg aag
//
LOCUS       DAZXUK010000079        15550 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZXUK010000079 DAZXUK010000000
VERSION     DAZXUK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15550
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.079"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..306)
                     /locus_tag="ACH3L8_001942"
     CDS             complement(<1..306)
                     /locus_tag="ACH3L8_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085073.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNVRHIFSNKDFVIKFSTSVLQADKKEITMINKNENTTLTQTIATI
                     "
     gene            complement(623..1447)
                     /gene="iclR"
                     /locus_tag="ACH3L8_001943"
     CDS             complement(623..1447)
                     /gene="iclR"
                     /locus_tag="ACH3L8_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFT4085074.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            1647..5330
                     /gene="metH"
                     /locus_tag="ACH3L8_001944"
     CDS             1647..5330
                     /gene="metH"
                     /locus_tag="ACH3L8_001944"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFT4085075.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5550..7181
                     /locus_tag="ACH3L8_001945"
     CDS             5550..7181
                     /locus_tag="ACH3L8_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFT4085076.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7272..7961)
                     /gene="pepE"
                     /locus_tag="ACH3L8_001946"
     CDS             complement(7272..7961)
                     /gene="pepE"
                     /locus_tag="ACH3L8_001946"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFT4085077.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8343..9572)
                     /gene="sorE"
                     /locus_tag="ACH3L8_001947"
     CDS             complement(8343..9572)
                     /gene="sorE"
                     /locus_tag="ACH3L8_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFT4085078.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            complement(9624..10412)
                     /locus_tag="ACH3L8_001948"
     CDS             complement(9624..10412)
                     /locus_tag="ACH3L8_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IID"
                     /protein_id="HFT4085079.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            complement(10423..11220)
                     /locus_tag="ACH3L8_001949"
     CDS             complement(10423..11220)
                     /locus_tag="ACH3L8_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFT4085080.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            complement(11286..11780)
                     /locus_tag="ACH3L8_001950"
     CDS             complement(11286..11780)
                     /locus_tag="ACH3L8_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFT4085081.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            complement(11780..12187)
                     /locus_tag="ACH3L8_001951"
     CDS             complement(11780..12187)
                     /locus_tag="ACH3L8_001951"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFT4085082.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            complement(12197..13003)
                     /locus_tag="ACH3L8_001952"
     CDS             complement(12197..13003)
                     /locus_tag="ACH3L8_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFT4085083.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(13073..14020)
                     /locus_tag="ACH3L8_001953"
     CDS             complement(13073..14020)
                     /locus_tag="ACH3L8_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFT4085084.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            14368..15240
                     /gene="rluF"
                     /locus_tag="ACH3L8_001954"
     CDS             14368..15240
                     /gene="rluF"
                     /locus_tag="ACH3L8_001954"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFT4085085.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(15373..>15550)
                     /gene="yjbD"
                     /locus_tag="ACH3L8_001955"
     CDS             complement(15373..>15550)
                     /gene="yjbD"
                     /locus_tag="ACH3L8_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFT4085086.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
BASE COUNT         3731 a         4006 c         4065 g         3748 t
ORIGIN      
        1 tattgtagca atggtttgag tgagtgtcgt gttttcgttt ttattaatca tcgtaatttc
       61 tttcttatca gcttgtaaga cggacgtgga aaatttaatg acaaaatcct tgttgctaaa
      121 gatatgacga acattttgtc tacagttctc tgagaagctt tttaatagag gcgtcgccag
      181 ctccttgcca gaaaatttat cctcgagttc tttataaaac aattcactca gggtttggtg
      241 ttcatttgtc tgggctgtat tattaatatt tgcagagaaa gaactacgag gaatacgagt
      301 aatcatttaa atatctcatt gtttattgat gtcttgaatt ttaactatca gaacagtgaa
      361 aaaatttaat atgattacaa ctaaaaaaat atcataaatc gctcaatctc ataatgcagc
      421 cgtaaaagtt tcggtggaat gagatcttgc gattttgtta ataattaagt ggtttgatgt
      481 ttaaaaataa tgctgagtta ataaaagtgt gtgaagtgta tgacattaag ttattttggc
      541 gtcaatgcga ttaacagaca cccttattct attgccactc aggtatgatg ggcagaatat
      601 tgcctctgcc cgccagaaaa agtcagcgca ttccaccgta cgccagcgtc acttccttcg
      661 ccgctttaat caccatcgcg ccaaactcgg tcacgcgatc atcggtaata cgtgaaatcg
      721 gtccggaaat agaaattgcg gcaaacggtt cgcggtgctc atcgaaaata cacgctgcaa
      781 ggcaacgtag ccccagcgca tgttcctcat cgtcaaatga ataaccccgt ttgcgcgttt
      841 gggcgagatc ttcttttaaa tgcacaggag acaccagagt tgcgtgggta taggcatgta
      901 accctttgcg gtgcagcagc ttcgtcacct gttcttcgct cagttgggct aaaaaggctt
      961 tacccgcacc ggaagcgtgc atcggcaatt taccgccgat aggcgcggac attcgcatca
     1021 ggtgggtaca ctgtacctgg tcgataataa tcgcttcgtg atcgctttga tcaagcaccg
     1081 ccatattgac cgtttcgcca gactcttcca ttagattgcg caggataggg tgaacaattg
     1141 ctaacaaatt acggctctgg agaaagctgc tgccgaccat aaaggcatgt gcgccgattg
     1201 cccagtgtcc cagttcgccg acctgacgca cgaaaccctg ctgttgcatc gtggttagca
     1261 ggcggtgggt cgtggaattg ggtaacccgg cttgttgcgc cagctctgtg agtgccacgc
     1321 tgccattgga ttcggcaatc cactccagta atttcaggcc acgcgttaaa gactgaacct
     1381 gtccagtcgc tggtgcggtg gcaacggcgg gttttctgcc gcgtttcgcg ggaatgggtg
     1441 cgaccatgac agcctccttt ttctgtatcg tggaaattat tttcattttt attgttagct
     1501 aatgcaatag ttgctgaact gatccgatga gttaatgttg aacaaatctc atgttgcgtg
     1561 gtggtcgctt ttaccacaga tgcgtttatt ccagtatggt ttgttgaatt tttattaaat
     1621 ctgggttaag cgtgtcggga gcaagtgtga gcagcaaagt ggaacaactg cgtgcgcagt
     1681 taaatgaacg tattctggtg ttggacggcg gtatgggcac catgatccag agctatcgac
     1741 tgaacgaagc cgattttcgt ggtgaacgct ttgccgactg gccatgcgac ctcaaaggca
     1801 acaacgacct gctggtactc agtaaaccgg aagtgatcgc cgctatccac aacgcctact
     1861 ttgaagcggg cgcggatatc atcgaaacca acaccttcaa ctccacgacc attgcgatgg
     1921 cggattacca gatggaatcc ctgtcggcgg aaatcaactt tgcggcggcg aaactggcgc
     1981 gcgcctgtgc tgacgagtgg accgcgcgca cgccggagaa accgcgctac gttgccggtg
     2041 ttcttggccc aaccaaccgc acggcgtcta tttctccgga cgtcaacgat ccggcattcc
     2101 gcaacatcac ttttgaccag ctggcggcgg cctatcgcga atccaccaaa gcgctggtgg
     2161 aaggtggtgc ggatctgatc ctgattgaaa ccgttttcga cacccttaac gccaaagcgg
     2221 cggtctttgc ggtgaaaacg gagtttgaag cgctgggcgt tgagctgccg attatgatct
     2281 ccggcaccat caccgacgcc tccgggcgca cgctctccgg gcagaccacc gaagcatttt
     2341 acaactcatt gcgccacgcc gaagcgctga cctttggcct gaactgtgcg ctggggcccg
     2401 atgaactgcg ccagtacgtg caggaactat cacggattgc ggaatgctac gtcaccgccc
     2461 acccgaacgc cgggctgcct aacgcctttg gtgaatacga tctcgacgcc gacacgatgg
     2521 caaaacagat acgtgaatgg gcgcaagcgg gctttctcaa tatcgtcggt ggttgctgcg
     2581 ggaccacgcc gcaacatatt gcagcgatga gtcgtgcagt agaaggatta gcgccgcgca
     2641 aactgccgga aattcccgta gcctgccgtt tgtctggcct ggagccgctg aacattggcg
     2701 acgatagcct gtttgtgaac gtgggtgaac gcaccaacgt caccggctct gctaagttca
     2761 agcgcctgat caaagaagag aaatacagcg aggcgctgga tgtggcgcgt cagcaggtgg
     2821 aaaacggcgc gcagattatc gatatcaaca tggatgaagg gatgctcgat gccgaagcgg
     2881 cgatggtgcg ttttctcaat ctgattgccg gtgaaccgga tatcgctcgc gtgccgatta
     2941 tgatcgactc ctcaaaatgg gatgtcattg aaaaaggtct gaagtgtatc cagggcaaag
     3001 gcattgttaa ctctatctcg atgaaagagg gcgtcgacgc ctttatccat cacgcgaaat
     3061 tgttgcgtcg ctacggtgcg gcagtggtgg tgatggcctt tgacgaacag ggacaggccg
     3121 atactcgcgc acggaaaatc gagatttgcc gtcgggcgta caaaatcctc accgaagagg
     3181 ttggcttccc gccagaagat atcatcttcg acccgaacat cttcgcggtc gccaccggta
     3241 ttgaagagca caacaactac gcgcaggact ttatcggcgc gtgtgaagac atcaaacgcg
     3301 aactgccgca cgcgctgatt tccggcggcg tatctaacgt ttctttctca ttccgtggca
     3361 acgatccggt gcgcgaagcc attcacgcag tgttcctcta ctacgctatt cgcaatggca
     3421 tggatatggg gatcgtcaac gccgggcaac tggcgattta cgacgaccta cccgctgaac
     3481 tgcgcgacgc ggtggaagat gtgattctta atcgtcgtga cgatggcact gagcgtttac
     3541 tggagcttgc cgagaaatat cgcggcagta aagcggacga cactgctaac gcccagcaag
     3601 cggagtggcg ctcgtgggac gtgaataaac gtctggaata ctcgctggtc aaaggcataa
     3661 ccgagtttat cgagcaggat actgaagaag cccgccagca ggctacgcgc ccgattgaag
     3721 tgattgaagg cccgttgatg gacggcatga acgtggtcgg cgatctgttt ggcgaaggca
     3781 aaatgttcct gccacaggtg gtcaaatcgg cgcgcgtcat gaaacaggca gtggcgtatc
     3841 tcgaaccgtt tattgaagcc agcaaagagc agggtaaaac caacggcaag atggtgatcg
     3901 ccaccgtgaa aggcgatgtc cacgacatcg gtaaaaatat cgttggtgtg gtgctgcaat
     3961 gtaacaacta cgaaattgtc gatctcggcg ttatggtgcc tgcggaaaaa attctccgta
     4021 tcgccaaaga agtgaatgct gatttgattg gcctttcggg gcttatcacg ccgtcgctgg
     4081 acgagatggt taacgtggcg aaagagatgg agcgtcaggg cttcgccatt ccgttactga
     4141 ttggcggcgc gaccacctca aaagcgcaca cggcggtgaa aatcgagcag aactacagcg
     4201 gccctacggt gtatgtgcag aatgcctcgc gcaccgttgg tgtggtagcg gcgctgcttt
     4261 ccgatactca gcgtgatgat tttgtcgctc gtacctgcaa ggagtacgaa accgtgcgta
     4321 ttcagcacgg gcgcaagaaa ccacgcacac caccggtcac gctggaagcg gcgcgcgata
     4381 acgatttcgc atttgactgg caggcttaca cgccgccggt ggcgcaccgt ctcggcgtgc
     4441 aggaagtgga agccagcatc gaaacgctgc gtaattacat cgactggaca ccgttcttta
     4501 tgacctggtc gctggccggg aagtatccgc gcattctgga agatgaagtg gtgggcgttg
     4561 aggcgcagcg gctgtttaaa gacgccaacg acatgctgga taaattaagc gccgagaaaa
     4621 cgctgaatcc gcgtggcgtg gtgggcctgt tcccggcaaa ccgtgtgggc gatgacattg
     4681 aaatctaccg tgacgaaacg cgcacccatg tgatcaacgt cagccaccat ctgcgtcaac
     4741 agaccgaaaa aacgggattc gctaactact gtctcgctga cttcgttgcg ccgaagcttt
     4801 ctggtaaagc agattacatc ggcgcatttg ccgtgactgg cgggctggaa gaggacgcac
     4861 tggctgatgc ctttgaagcg cagcacgatg attacaacaa aatcatggtg aaagcgcttg
     4921 ccgaccgttt agccgaagcc tttgcggagt atctccatga gcgtgtgcgt aaagtctact
     4981 ggggctatgc gccgaacgag aacctcagca acgaagaact gatccgcgaa aactaccagg
     5041 gcatccgtcc ggcaccgggc tatccggcct gcccggaaca tacggaaaaa gccaccatct
     5101 gggagctgct ggaagtggaa aaacatactg gcatgaaact cacagaatct ttcgcgatgt
     5161 ggcctggtgc atcggtttcg ggttggtatt tcagtcatcc ggacagcaag tactacgcgg
     5221 tggcgcaaat tcagcgcgat caggttgaag accatgcccg ccgtaaaggt atgagcgtct
     5281 ccgatgttga gcgctggctg gcaccgaatc tggggtatga cgcggactga ttcacaaatc
     5341 tgtcactttt ccttacaaca aacagggcgc tcaatgagtg ccctgtctct ttattaatat
     5401 gaaacactta tactggaaac aggctggaat aatcttagcc ggaaaaacga atgtcaaaag
     5461 gcacagtaaa gatgccagtc agcgtgtttc cggtcctttg gtcattcaat tgcttcagga
     5521 acatataacg ataaggagaa tctgaactcg tgttaacgct gcttcacctg ctttctgccg
     5581 tcgccctgct ggtctggggg actcatattg ttcgaaccgg cgtaatgcgc gtcttcggcg
     5641 cgcgtttgcg tactgtcctt agccggagcg tcgaaaaaaa gccgctcgcc ttttgcgcgg
     5701 ggatcggcgt taccgcactg gtacagagca gtaatgccac caccatgctg gtgacctcgt
     5761 tcgtcgctca ggatctggta gccctcgcac cggctctggt cattgtgctg ggggcagatg
     5821 tcgggacggc gctaatggcg cgtattctca ccttcgactt atcctggctg tcaccgttac
     5881 ttatttttat cggcgtgatt tttttcctcg gacgcaaaca gtcacgcgcc gggcaactgg
     5941 gccgcgtcgg tattggtctt gggctgattt tgctggcgct ggagttgatt gtgcaggccg
     6001 taacgccgat cacccaggca aacggcgttc aggtgatctt tgcctcgctg accggcgata
     6061 ttctgctgga tgcgctgatt ggcgcgatgt tcgccattat cagctactcc agccttgctg
     6121 ctgtactgct gaccgcgact ctgaccgctg caggcattat ctccttcccc gtggcgctct
     6181 gtctggtgat tggagctaac ctcggttccg gcctgctggc gatgctcaac aacagtgccg
     6241 ccaatgccgc agcccgccgt gtcgcgctgg gtagtctgct gtttaagctg gtgggtagcc
     6301 tgattatcct gccgtttgtc catttgctgg cagagacaat ggggaagctg tcattgccaa
     6361 aagcggaact ggtgatctat ttccacgtct tctacaacct tgtacgttgc ctggtcatgc
     6421 tgccatttgt tgacccgatg gcacggtttt gcaaaacgat tattcgcgat gaaccggaac
     6481 tggataccca gctacggcct aaacatctgg atgtcagcgc gctggacacg cccacgcttg
     6541 ctctggcgaa cgccgcgcgc gaaaccctgc gcattggcga cgccatggaa cagatgatgg
     6601 aagggctgaa taaagtgatg cacggcgagc cacggcagga gaaagagctg cgtaagctgg
     6661 cagatgatat caacgttctc tacaccgcca ttaagctgta tctggcgcgg atgccaaaag
     6721 aagagttggc ggaggaagag tcgcgccgct gggcggagat catcgaaatg tcgctcaacc
     6781 ttgaacaggc ctccgatatc gtcgagcgca tgggcagcga aattgccgat aaatcgctgg
     6841 cagcacggcg ggcattttcg cttgatgggt tgaaggaact ggatgcgctc tatgagcaat
     6901 tgctcagtaa tttaaagctg gcaatgtcgg tgttcttctc tggcgatgtc accagcgctc
     6961 gtcgtttgcg tcgcagcaaa catcgttttc gcattcttaa tcgccgctat tcccacgccc
     7021 acgtcgatcg cctgcatcag caaaacgtgc agagcattga aaccagttcg ctacatttag
     7081 gcttactggg agatatgcag cgcctgaact cgctgttttg ttcggtggct tacagtgtgc
     7141 tggaacagcc ggatgaagat gaaggacggg acgagtatta atattgtgaa tcctgtaggc
     7201 ctgataagac gcgtcaagcg tcgcatcagg cactatgaac aagatgccgg atcatgcatc
     7261 cggcaattca cttaaaaacg gtgaccagct tccagcggaa ccgcttcttc accagcctta
     7321 aacacataag tggtgttcgg gccacccagc gtagcgtgac ctttactcac tgtgatccag
     7381 ttaccttccg gcagaccaat aatcgtcagt tctggcgcga cgaccagcag ttcgcgaata
     7441 cgctgctcac gggtttcacc tttatggcct tccggcagcg cgttggtgaa gtgcgggttg
     7501 atttgcaacg ggaacagatt tagcgcatcg aaaccttgcg gatcgacaat cggcatatcg
     7561 ttggtggtac gaatggttgg gcaagcaagg ttagcgcctg cgctccagcc aatatacagc
     7621 gcgccacgtt taaccacgtc agtaattgga gccagcagcc cgcgctcgcg gcactgtttc
     7681 agcaactgga aagtattacc gccgccgaca atcacaattt ccgcattttc aatcgcggca
     7741 acgggatcga caacgctatg aataccggtg acagaaacac ccagcggagc gagaaccgca
     7801 gccgttttct ctgtgtaatc atcccaggtc tgcgttacgc cagcgaaagg gataaacacc
     7861 gctgagcggc gaccctgcaa ctgttcagca attagcggca gtgcatgttc cagccaggct
     7921 ttacccggca gcgtcgagtt actcaataaa agcagttcca ttacttctcc ggttagtaag
     7981 aaatcacgga aggcatgcta ccactgcgaa tttaaccact tactgacatg gctcacgatg
     8041 tcacgtattg caccgggcgc tgagactgat gacaaacaca gcattgcctg atgcgctacg
     8101 cttacctggc ctaccagata actgcaatat attggatttt caaaattatg tagaccgaat
     8161 aaggcttcat tccgcatccg gcataaacaa tgtgtacttt gccaccaatt tgagcgcccc
     8221 ttaaggcgct gatatttttt atccttctcg tgcaaattgt gctgcttcag caatcagccg
     8281 cacatccggt cgcgaagttg ctggggcgac cagccagcga catgcagagt taagtagcgc
     8341 gattaatcat gggaaattgc ctctgcatgg gcgagcaggt attgctccac ctcaccggac
     8401 cagatcccct gatgacgcgc cagaatcgcc gccagtgctt gatcctgaat ctgcgtgagt
     8461 gacgtcagcg gcaggtattt cccggtatac accagctttt tgccgccgcc gacggcaggc
     8521 aattcaagcg tggtttcgcc cgcggcattc agcccaagaa tatgtgttac cacttttgcg
     8581 gcctgcactt ttttctcttc gatcagtttg accgccgcgc gcatgtcgtc ggtattgccg
     8641 cctgacgtgc ccacgtagtg ggtaaatgca taatgcacat cgtagaaatt aattggcgcg
     8701 ctgaaatgtt tatcctgcgg tccggcgaag aaattcaggc aaccatctgt cgccagcaag
     8761 gaagaggcga gagtcaccag tccttcatta ggcacaaaga cgaaaatatc atcgaagccg
     8821 tgaccgccac tcagcgccat taacgtatca aatgctgcat cggtggcatt gagataatga
     8881 atcagtgttt gcggttctga tggatagtgc ttgcgcgcat aactcaattt gtcgttgtcg
     8941 gtatcggtaa tgacgagcag cgacgggtta actggtccat gcagcgcata atcaatcgcc
     9001 aacagtccca ttggtccggt gccgccgagg atcagcgtgt gcccttgcgg gcgaatcccc
     9061 atcgtgtggt tataactacc ttcctgaaga tgatagttgg cgttgaacgc gccaatcacg
     9121 caggaaagcg gttcaaccag tgagccttca aaataggttt cgccgtcgta tgccagcagg
     9181 caatattgtt ccatgacctc gttgggaata accacatgcg tcgcttcgcc gtctacccac
     9241 gggaaggagt agccggggca atccgggcgg tcggggagtt gcaggttggc ctgaatcaca
     9301 taacgctgac ccggctggaa tttgtgctgc cactttttac ccacggccag aatatcgccg
     9361 caaaattcgt ggccgatgat gattgggttg gtcgtcacat cgtcgggtac ttttttatgg
     9421 ttttcaccca gattggcctc tttccaggaa gagaggcaca ggctgtcagt gaccaccgtt
     9481 gcgaggattt catcctcctg catttcagga aggtcaaagg tttccaggcg taaatcacgt
     9541 ttaccataaa gacgcagagc tgttgttttc ataaaccacc tcatgttgag cgatggcctc
     9601 ggcagtcgtg ccggggcgaa gaatcaggac aggaagccca gagcattgcc gataatccct
     9661 aagccaaaca gggcgaaaat cagccatacc gggttaattt ttttgttgag cagacgaacc
     9721 attagtagcg tcagaccgag cgccagcaaa ccagggcaaa gttggtcgag aatgttctgc
     9781 acggtcatgg tgacggtgga gccatcggcg gcatgcgttt gtgaaaccac caacggtacg
     9841 ttgattgacg tccatttggt caccagcacg cccatcacaa acagtccgag aatcgacgcg
     9901 ccttcggtga gtttttgcag cacattcccg cccatatcgc tgacgatatt cacccctttg
     9961 cgaaagccga gctgtaggcc ataccacttc atcgccagac gcaccgcgtt gaaaataaag
    10021 aagaacagca gcgggccgag aatgttgccc gaaagtgcca gagatgcgcc gagcgcggcg
    10081 gtaatcgggc gcagcgttcc ccagaccagt ggatcgccaa ctcctgccaa tggtcccatc
    10141 agaccgactt tgatgccgtt gatggtaccg tcatcaattt ccgcgccgat gaccgggcca
    10201 catacggctg gcgtggtatt gaagaacacc aggtgtagcc tgagcgccgc aacctgatcc
    10261 tcttttaatg ggtaaagtcg cttgatggcg gggatcatgt cgtagcaaaa gcccagcccg
    10321 tgaatacgtt caaagttaaa ggacgcctgt tgcaggttgg agcgcagaaa catgctcacc
    10381 agatcgctgc gtgtaatttt tctctgttcc atgatggctc cgttaatcgt caagttggtc
    10441 aagggcggtg gaggcggtgg cctggagttg aggttcagct ttacaccact gtggatttag
    10501 ctggatgtag accagggcca tgatcacgcc gacgccaccg aacgccagca ggctgagatc
    10561 gaggtagcca cctgcgagga aaccgaggaa aaagaaaggc atcaaatatt tcacgcccat
    10621 catacgtagc accatggcgt aaccgaccac cacgataaaa ccgccagcaa tctgtagtcc
    10681 tcgggtcaca aattcgggaa tggcgtttaa catattgctg accatatcgg cgctgacgaa
    10741 cagtgagaca atcagtgccg gaatggcgac gcgcagcgcc tgcacgccaa gcgcggagac
    10801 atgcagaata tcgagagtgc gaaaccgcgc ctcttctgct gctttatccg ccgcgtgctg
    10861 gaacactacg gtgatggtac gggcaaacac cgtcagcacc tggcctgccg cagccacagg
    10921 caacgcgatg gcgattccgg tggcgatgct ttgctgacca acgataacca gaatggcgga
    10981 gattatgctg gcgagtgcag aatccggaga ttgcgccgcg ccgacgttca tccagcagag
    11041 agctatcaat tccagcgtac cgccgagtat tattccggtt tttaaatcac cgagaattaa
    11101 accaatcacc gtacaggcaa ttaacgggcg atgagtctga aattcatcca gcacgctgcc
    11161 cattccggca atacaggaaa aaagaaatat agcaatgatt tgtagggtac ttatttccat
    11221 gatttgtcac cttttaagtg tcgtcatccc tgcctgttaa agcatatgta cacaggcgct
    11281 attatttaat ttgcgattaa ctgttcgttt attttgtcga taatattgat tgagggatct
    11341 gacgctacga cgcgtaaatc cagtatgaca ccaagattat ttaactcatg aaatgcgtta
    11401 atatcatcat catctaatga aaccgctttg gttaactgtt ttttaccggg ccgccaggcc
    11461 atgccgccaa tattcagggt gcctattttt accccctggc gaaccatcgc taaggcatcc
    11521 tgtgggcgag tgaaaagata aaaaacggtt tcgtcctgat attgcgggtt gtgataaacc
    11581 gcgacggctt tttcaatatt gaccacgtta accttcattc ccggtggcgc agcctgacgc
    11641 aataatgtcc ggcgcacttc atcgttataa acttcgtcat tgcagataat aatgcgctgg
    11701 gcgttagcca ctttcgacca gacggtagtg acctggccgt ggatcagacg gtcatcaatg
    11761 cgggcgagcg taatgttcat cagaaatcct cctctgtcgt tgccagttgt ttccagacaa
    11821 cgcaggtttg ctttgcgatt tgcgtcaggt gttcacacag ttcatcaaca ttcatgctgt
    11881 cctggttatc aaccagttcg agcgccagcg gcagagaaag gccgctgatt acccgcaaac
    11941 gaggattgcg catcgcaagc attgctgccg cattccacgg gctgccgcac tgtaaatcca
    12001 cggcaatcag ccactcatct tgattgtgaa tgcttactaa cttttccagc tttaccgcaa
    12061 tgtctccggc gttttcaccg ggcacaaatg caacggcttc aacattggcg ttgccgtaaa
    12121 ccatctgaac cgattccagc atggcgcaag ctaatttgcc gtgggcacaa aaaatggcgt
    12181 tgaccataaa gcctccttag ccgcgcgttt tgccgcccgc aatgttagtg gttactccgg
    12241 tgatgtagct ggcgcgttca gacaacagat aacaaacaaa atcagctact tctgctaatc
    12301 ttccggcgcg cccaatagga atggcgtttt tggtatagcc ttcacgcagc tgctcgacgg
    12361 tgatattgcg cgtccacgcc agcgcctctt catattccgg cgtgcgtagc ccggtttttt
    12421 ccagaatccc gggcgcgata ccgaccacac ggataccgtg tttacccagc tctttcgacc
    12481 aggagcgcgt gaagctattg agcgcggctt tggtagcggc gtaacagctt tgtccttctg
    12541 agccttccag cccactttct gaggaaacat tcacaatcac gccatcatgt tgtttgacca
    12601 tctgtcgcgc caccgcctgc gacatcagaa aaacaccttt ctggttgata ttgaccattt
    12661 tttcgaatgc agcttcgttg agttcatact gcccggcagg cgctttctca tcgaccagca
    12721 gacgcgggaa attgaccccg gcgttattga ccagaccgtc gatgcgacca aaacgctgga
    12781 taatttccgc taccgtatga tttacctctt ttgtgctaga aatatcggtc ggccaaaact
    12841 gatagccttt atgaccttca tattgaccat cgccaccgtg aatatcgacc atctgtacat
    12901 tcgcgccttg tgctaataat tcctctacaa tcgccagacc aataccggat gcgccgccgg
    12961 tgacaataat gattttatcc tgcaaattta accacgtttg cataacagac tcctgatttt
    13021 agataatagg gacccgccgt aatttttatt acggtaaaca tcgggaagat ttttatttca
    13081 gtaatagttc agctgtgctg ctattcgtta ccagacaatt aatatatttt ccacgcagtg
    13141 caccaataat tccgctgtat ttttcttcac tcatggcaat gccaatggaa tatcgcgcct
    13201 gctttaattt attcatttcg atagagagcg ttttttcgct catattcgtt tcaaccattg
    13261 cgccgtgaat atcaaaaaag cgcgagcaaa tatcgccagc aacctggcgg gcattcaggt
    13321 cgtcactctc ttcaccacca taaaacgcat gccagttagc gccgtcgcga atggccggtg
    13381 agccaattcc caccagggcg atatccagat tatcccagta ggcagagatg gttttaaagt
    13441 gctgagactg catgatccca ttacgaatta atgggttatc cagcagagcc ggaaaatccg
    13501 cgagatgcga ttcccctttc agcttcgctg ccgcgctgta ggttaatgtg tttacatgat
    13561 agcggctttc gagtttaccg gacggtccgc caataattgg tacgcagatt aactgccgcg
    13621 attgccccgc ctgcggcaag ttttcaacca gtgcgctcac cgcgcgaccc caggaaaaac
    13681 cgacaatatc gccaggctcc agcaagcgat ccagcagttg cgcgccgtgt aaccccatca
    13741 tcgccagttg agtctcttca tcctcatcat ttcccgacac caccacaacg tctttcaggc
    13801 caaacttttg cttcagttgc tgctccagcc agagattttc gttgtagtca tagttgatgg
    13861 cgatggtgac cattccctga tcgcggcctc gtttaagcaa gcggctgatg gtggtgcggt
    13921 aaatacccag ttcgcgcgcg atttgcgcct gcgtcatatc ctgttcgtaa tagagttggg
    13981 caatcttcac aatcaaacgg atatcgtcac tgttttccat tgccgcctcc tggttctgca
    14041 catttgtgca aatacgcttt ctcaccgctt gatctgatat aaccccatca tgagggcctt
    14101 tcaaagcgat ttttcactct ttgcatgggg agaaatagcg ctttctcttg cacatttgtt
    14161 tctctgcgta gtagtcatca gcacaggtgt gcagcgattc gtaatatgtt gattatcctt
    14221 attttgccag gcaaatttgc atcgtacttc cggagacact tttgtgataa aaggcgtggc
    14281 agatcacatt taatttcgac gttgccgcat aaccctgttt ttccccgcta agaggtacgg
    14341 tatattccgc gttttacagg agaatttatg ctgcccgact catcagtccg tttaaataaa
    14401 tacatcagcg aaagcggaat ttgctcacgc cgcgaagcgg atcgctatat cgagcaaggc
    14461 aatgtgttcc ttaatggcaa gcgagccacc attggcgatc aggtgaaacc cggcgacatt
    14521 gtgaaagtaa acggtcagtt gattgagcct cgggaagccg aagatttggt acttatcgcc
    14581 ctgaacaagc ccgtcggtat tgtcagcacc accgaagatg gcgagcgcga taacattgtc
    14641 gatttcgtta accacagcaa acgcgtgttc ccgattggcc gcctggataa agactcccag
    14701 gggctgattt tcctcaccaa tcacggcgat ctggtgaata agatcctccg tgcgggtaac
    14761 gatcatgaga aagagtatct ggtgacggtc gataaaccga ttaccgatga atttattcgt
    14821 ggtatggggg cgggggtgcc gatcctcggg acggtgacga aaaagtgcaa agtcaaaaaa
    14881 gaagcgccgt ttgtcttccg cattacccta gtgcaggggc tgaaccgtca gatccggcgc
    14941 atgtgcgagc atttcggcta tgaagtgaaa aagctggaac gtacgcgcat tatgaacgtc
    15001 agcttaagcg gcattccgct gggggagtgg cgcgatttaa ccgacgatga gttaatcgac
    15061 ctctttaagc tcatcgaaaa ttcctcttcc gaggcaaaac ctaaagcgaa ggccaaaccg
    15121 aaaacagcgg gcatcaaacg tccagtcgtt aagatggaaa aaacggcgga aaaaggcggt
    15181 cgcccggcgt ccaacggtaa gcgttttacc tcgccggggc gtaaaaagaa gggacgctga
    15241 ttaacgcctc agactgatga aaacgtccaa aaagagctcg gacagtcccc tcgccccttc
    15301 ggggagaggg ttagggtgag gggaagattg tgtccacaca gacgagtgaa aatctcaggc
    15361 gttgattgtt cattagcgcc tgccgcgcgt cgacgtattt gctgaccagc taaatgacgc
    15421 ttcaggatcc ggtttataag cctgcttttt cttcaattgg cgggcttttt tcgcttcagc
    15481 ctcacgctcg accattaatt tatcgatgta ctctttctta atgctgttgg tttcggagtt
    15541 ggtcagtacg
//
LOCUS       DAZXUK010000080        15528 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZXUK010000080 DAZXUK010000000
VERSION     DAZXUK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15528
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.080"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            2..223
                     /gene="yjbE"
                     /locus_tag="ACH3L8_001956"
     CDS             2..223
                     /gene="yjbE"
                     /locus_tag="ACH3L8_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFT4085087.1"
                     /translation="MFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSATSVSTVS
                     SAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            337..975
                     /locus_tag="ACH3L8_001957"
     CDS             337..975
                     /locus_tag="ACH3L8_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFT4085088.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            972..1709
                     /locus_tag="ACH3L8_001958"
     CDS             972..1709
                     /locus_tag="ACH3L8_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFT4085089.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1709..3805
                     /locus_tag="ACH3L8_001959"
     CDS             1709..3805
                     /locus_tag="ACH3L8_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFT4085090.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3852..4130)
                     /locus_tag="ACH3L8_001960"
     CDS             complement(3852..4130)
                     /locus_tag="ACH3L8_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085091.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4131..4274
                     /locus_tag="ACH3L8_001961"
     CDS             4131..4274
                     /locus_tag="ACH3L8_001961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085092.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4400..4810
                     /gene="psiE"
                     /locus_tag="ACH3L8_001962"
     CDS             4400..4810
                     /gene="psiE"
                     /locus_tag="ACH3L8_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFT4085093.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4854..6329)
                     /gene="xylE"
                     /locus_tag="ACH3L8_001963"
     CDS             complement(4854..6329)
                     /gene="xylE"
                     /locus_tag="ACH3L8_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFT4085094.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6700..7590)
                     /gene="malG"
                     /locus_tag="ACH3L8_001964"
     CDS             complement(6700..7590)
                     /gene="malG"
                     /locus_tag="ACH3L8_001964"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFT4085095.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7605..9149)
                     /gene="malF"
                     /locus_tag="ACH3L8_001965"
     CDS             complement(7605..9149)
                     /gene="malF"
                     /locus_tag="ACH3L8_001965"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFT4085096.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9303..10493)
                     /gene="malE"
                     /locus_tag="ACH3L8_001966"
     CDS             complement(9303..10493)
                     /gene="malE"
                     /locus_tag="ACH3L8_001966"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFT4085097.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10858..11973
                     /gene="malK"
                     /locus_tag="ACH3L8_001967"
     CDS             10858..11973
                     /gene="malK"
                     /locus_tag="ACH3L8_001967"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFT4085098.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12045..13385
                     /gene="lamB"
                     /locus_tag="ACH3L8_001968"
     CDS             12045..13385
                     /gene="lamB"
                     /locus_tag="ACH3L8_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFT4085099.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13537..14457
                     /gene="malM"
                     /locus_tag="ACH3L8_001969"
     CDS             13537..14457
                     /gene="malM"
                     /locus_tag="ACH3L8_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFT4085100.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14686..15528
                     /locus_tag="ACH3L8_001970"
                     /pseudo
     CDS             14686..15528
                     /locus_tag="ACH3L8_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT         3924 a         3905 c         4014 g         3685 t
ORIGIN      
        1 catttttgcc atatctgcgc ttgcggcgac ttctgcgtgg gctgcacctg tacaggtggg
       61 cgaagcggca gagtcggcag caacgtcggt ttcggcgggg agttcctccg cgaccagcgt
      121 cagcaccgta agctcggcgg tgggtgtcgc gctcgcggca accggtggcg gtgatggttc
      181 taataccggg accaccacaa ccacgaccac cagtacccag taataaagta tgtatcccca
      241 aaataatttg agtcattgca tctgtggctt gaagtatgaa gggatttacc ataaccacac
      301 tccggtgtgg ttattctgcc cctctggaga agagtcgtga agcgacctgc actcattctt
      361 atctgcctgc tattacaggc ctgttcagcc acgactaaag agctgggcaa ttcactgtgg
      421 gacagtctgt tcggcacgcc aggcgtacag ctgacggacg atgatattca aaatatgccc
      481 tacgccagcc agtacatgca gcttaatggc gggccgcaat tgtttgtggt gctggccttc
      541 gctgaagacg gacaacaaaa atgggtcact caggatcaag ccactcttgt cacacaacat
      601 ggtcgtctgg tgaagacttt gcttggcggc gacaacctga ttgaagtgaa taaccttgcc
      661 gccgacccgc tgattaaacc cgcacaaatt gttgatggtg caacatggac gcgcacgatg
      721 ggctggaccg agtaccagca ggtacgctac gccaccgcac gctcagtctt caaatgggat
      781 ggcaccgata ccgtcaaggt cggcagcgat gaaaccctgg ttcgcgtgct ggacgaagaa
      841 gtctccaccg accaggcgcg ctggcataac cgctactgga tcgacagcga agggcaaatt
      901 cgccagtcgg aacagtatct cggcgcggat tatttcccgg tgaaaaccac tctcatcaag
      961 gcggcaaaac aatgattaaa caaactattg tcgcgttgct tttgagtgtg ggagcgtcat
     1021 cggtcttcgc ggcaggaacc gtcaaggtgt tcagcaatgg cagcagtgag gccaaaacgc
     1081 taacgggcgc agagcattta atcgatctgg tagggcaacc tcgactggca aacagctggt
     1141 ggcctggtgc ggtgattagc gaagagctgg caacggcggc agcattgcgt cagcagcagg
     1201 cgttgctgac acggctggca gaacagggcg cagattccag caccgacgat gccgccgcca
     1261 ttaacgcctt acgccagcaa atccaggcat tgaaagtgac aggcaggcaa aaaatcaatc
     1321 ttgatcccga tatcgtccgc gttgccgaac gcggtaaccc gccgttgcag ggcaactaca
     1381 cgctgtgggt cggaccaccg ccgtccacgg tcacgttgtt cgggcttatc agccatcctg
     1441 gcaatcagcc attcactcca ggtcgcgacg tggcgagcta tctctctgac cagagcctgc
     1501 tcagcggtgc ggatcgcagc tacgcgtggg tcgtttaccc ggacggacgc acgcaaaaag
     1561 cgccggtggc ttactggaac aagcgtcacg tagagccgat gcccggcagc attatttatg
     1621 ttggcctcgc agattccgtc tggagtgaga cgcctgatgc ccttaacgcc gacattcttc
     1681 agactctgac gcagcggata ccccaataat gaaaaaaaga catctgctta gcttactggc
     1741 gctgggcatt agcacagctt gctacggcga aacatatcct gcgcccattg gtccgtcgca
     1801 gtcggatttc ggtggcgtag gattattaca aactcccacc gcgcgtatgg cacgggaagg
     1861 ggagttgagt ctgaactatc gcgataacga tcagtaccgt tattactcag cttcagtgca
     1921 actcttcccg tggctggaaa caacgctgcg ctacaccgac gtgcgcaccc ggcagtacag
     1981 cagcgtcgaa gcgttctctg gcgatcaaac gtataaagat aaagccttcg atctcaaact
     2041 gcgtttgtgg gaagagagtt actggctgcc gcaagtggcg gttggtgcgc gggatatcgg
     2101 cggtacgggg ctgtttgatg cggaatatct tgttgccagc aaagcctggg ggccgttcga
     2161 ttttacgctc ggcctgggct ggggatatct gggcaccagc ggtaatgtaa aaaatccgct
     2221 ctgttcagcc agtgataaat attgctatcg cgataacagc tacaaacagg cgggatctat
     2281 cgacggtagc cagatgttcc acggtcctgc ctcactgttt ggcggcgtgg aataccagac
     2341 gccctggcaa ccgctgcgtc tgaaactgga gtatgaaggt aataattatc agcaggattt
     2401 tgccgggaag ctggagcaaa aaagtaagtt taacgtcggg gcgatttatc gcgttaccga
     2461 ttgggccgac gttaacctta gctatgaacg tggcaacacc tttatgtttg gcgtcacgct
     2521 gcgcaccaac tttaacgatc tgcgcccgtc ctacatcgat aacgcccgcc cgcaatatca
     2581 accgcagccg caggatgcca ttttgcagca ttcggtggtg gcgaatcagt taacgttgct
     2641 gaaatacaac gccggacttg ccgatccgca gatccaggcg aaaggcgata cgctgtatgt
     2701 taccggcgag caggtgaaat atcgtgattc gcgcgaaggg atcatccgtg ctaatcggat
     2761 cgtgatgaac gatctgccgg atgggatcaa aacgatccgc attacggaaa atcgccttaa
     2821 catgccgcaa gtgacgacgg aaaccgatgt cgccagcctg aaaaatcatc tcgccggaga
     2881 gccgttgggc cacgaaacga cgctggcgca caaacgcgtc gagccagtgg ttccgaagtc
     2941 caccgagcag ggctggtata tcgacaaatc acgctttgat ttccatatcg atccggtgct
     3001 gaaccagtcg gtcggtggcc cggaaaactt ttacatgtat cagctgggcg tgatgggaac
     3061 ggcagatttg tggctgacgg accatctact gaccaccggc agcctgtttg caaatcttgc
     3121 caacaactac gacaagttta actacaccaa tccaccgcag gactcgcact taccgcgcgt
     3181 tcgtacccat gtgcgcgagt atgtgcagaa cgatgtctat gtgaataacc tgcaagccaa
     3241 ctacttccag catctgggca acggcttcta cggtcaggtc tatggaggtt atctcgaaac
     3301 catgtttggc ggtgcggggg ccgaagtgtt gtatcgtccg ctggacagta actgggcgtt
     3361 tggtctggat gccaactacg ttaaacagcg cgactggcgt agtgcaaaag atatgatgaa
     3421 attcaccgac tacagcgtga aaaccggaca tctgaccgcc tactggacgc catctttcgc
     3481 ccaggacgtg ttggtgaaag ccagcgtcgg gcagtatctg gcaggggata aaggcggcac
     3541 gctggagatc gctaaacgct ttgacagcgg cgtggtggtg ggtggctatg ccacgatcac
     3601 caacgtttcg aaagaggagt acggcgaagg ggacttcacc aaaggcgtgt atgtctcggt
     3661 accgttggat ctcttctcgt ctggcccgac acgcagccgt gcggcgattg gctggacgcc
     3721 gctgacgcgt gacggtggtc agcaacttgg gcgtaagttc cagttgtatg acatgaccag
     3781 cgaccgtagc gtaaatttcc gctaagtcat gggaaaggtg ccagttttcg cactcactgg
     3841 caccctcatt cttaatgacc tgttgagcca tcaggaacgc agttcacccc cggtttacga
     3901 tctggtgtgt aaaggcgaaa acattctgtc ttcctgtccg tcgatatcgg ttgcaggtct
     3961 ggttgcctga gcgactgggc catcattggg ctggagacat tgctttgcgg taggtcggct
     4021 ttatcagcag taccgagcgg accagcataa gcaggaagaa cagagactga taacatcaaa
     4081 gcagcaaaat aaggcttcat ttttaccacc tttatcaggt tacgtttcat ttgttccaga
     4141 ggaacattgt cgatttttcg cgcattgctg gtggctggga atcacctgaa tgggtgattt
     4201 ttgaattacc ggctttggtg cggtttgtct tgccggatgc gccgccaggc gcggcttatc
     4261 cggcctacgg gtaggtatat ccggctttgg tggaggcgcg ctccaaatcc aggttgaaca
     4321 aaacatacac aaaaaatata gatctccgtc acatttttgc gttatacagg aagctcgcca
     4381 ctgtgaagga ggtactgcta tgacgtcact ctctcgtccg cgcgtggagt ttatctccac
     4441 cattttgcag accgtactca atcttggcct gctgtgcctc ggcctgattt tggttgtctt
     4501 cctcggcaaa gaaacggtgc atctggctga tgtgctgttc gcgccagaac aaaccagcaa
     4561 atatgagctg gtagaaggac tggtggttta ctttctctat ttcgaattta tcgcgctgat
     4621 tgtgaagtac tttcagtccg gttttcactt cccgctacgt tactttgtct atattgggat
     4681 caccgccatt gtgcgcttga tcatcgtcga tcataaatcg ccactggatg tgttgatcta
     4741 ctcggctgcg atcctgttac tggtgatcac cctgtggctg tgtaattcga aacggctgaa
     4801 gcgggagtaa aaagacagca cgccgaaatg gcgcggcgtg ctggacagga agattacagc
     4861 gtagcagttt gttgtgtttt cttcgtttcc ggttcccaga gcgcttccag ctcctcaagg
     4921 gttttacctt tggtttccgg gacaaatttc cacataaaca gtgctgccag aacgcccata
     4981 caaccgtaaa tccagtagga gaaaccgttg tggaaatggg ccaccagcca ggagtttttg
     5041 tccatcatcg ggaaggtcca ggagacgaag tagttcgcca gccactgggc cgccaccgcg
     5101 attgccagcg ctttaccacg aatagcattc gggaagattt ccgacagcag tacccagcat
     5161 accggacccc aagacatggc aaaggcggca acatagaaca gcatcgacag tagcgccaca
     5221 atacccgatg cctgagtgta aaacgcggta ccgaggctaa acataccgat tgccattccg
     5281 agtgcgccga taatttgcag tggcttacga ccaaatttat ccacagtcat aattgccagt
     5341 acggtaaagg tgaggttgat aactccgaca ataatggtct gcaacagcgc gatatccgtg
     5401 ctggccccca gcgttttgaa cacttccggc gcgtagtaca gcaccacatt gatgccgaca
     5461 aattgctgga agatggagag cataacgccg attacaatca cgcccacgcc aaacatcagc
     5521 agacgaccac cggttttgcg gccatgatcc agggagtgtt taatttcctg tactgcctga
     5581 gttgcaagcg tgttgcccat aattttgcgc aggatacttt ccgtctgttc ttgcttgccg
     5641 cgtgacatca gccagcgagg actttctggc acggtataca gcagcattaa gaacagcagt
     5701 gcagggatac attccgaggc aaacatataa cgcctgccgt cagtattcag ccagctggca
     5761 tcaccggaac gggcaataaa atagtttacg cagtaaacta aaagttgccc gaaaataatc
     5821 gcaaactggt taaatgaaac cagttttccg cgaatatgag ctggagccag ttccgcaata
     5881 tacattggtg aaagcattga ggctaaacca acgccaatac cgccaataat acgataaata
     5941 acaaattccg ggacataacc tgccagataa ataggtactg tgttgtccgg gtttatagag
     6001 gtaaaaccaa gttctggcca ggcagaacct acaccagaaa taaaaaacag gacagcagaa
     6061 atcttaagtg aatcacgacg gccgaagcgg ttactgcaat aaccaccgag ggcaccgccg
     6121 atgatgcaac caatcagagc gctggctacg caaaatccta acagggagtt ggcagcggat
     6181 tcacttaagt tttgtggagc aacaaagacg gtattgagtg actcaacagt accggaaata
     6241 acggcggtat cgtagccaaa taataaacca cctaatgtag cgactaaggt aatcgaaaat
     6301 atataactgg aattatactg ggtattcatt cagacctgcc ttagaccatt ctgatctttt
     6361 atcagtgcat tgatgttgga attgtcaggc agttaaggca attatcattt tttacaatgc
     6421 gtttacgtaa tgtcttttct gtgatcttaa ttgtgataat tatcaaaaat tgatgaaaca
     6481 atactattgc cgtgactcag agcacgaaag agaattatcg taagtgggaa aacaaataac
     6541 gtaaaaataa taagttctat gatgggaata ttaaccggcg aacgattcag attgcagacg
     6601 aaagaaaaaa aggcgctccg tggagcgccg aataatagtc acaagttggg ataacgtaag
     6661 ttgagggtgc agcggcataa cattggcaga acaacatctt taacctttca caccacctgc
     6721 cgtcaggccg ttgaccagcc agcgttgagc cagcaagaag acgatggtga tcggtaatgc
     6781 agacatcacg gcagcggcgg caaagtcacc ccacaggtag ttttgcgggt tgaggtattg
     6841 ctgcatcccc acggccaggg tgtagctgtt tacgtcacgc agtaacagcg acgcgaccgg
     6901 aacttcagta atggcagcga taaacgacag gatgaatacc accgccagaa tcggtactga
     6961 cagcggcaac aggacaaggc ggaaggcctg ccacggtgtc gcaccatcca gcgcagccgc
     7021 ttcttccagc gaactgtcga tggtttcgaa atagcctttg atggtccaga catgcagcgc
     7081 aatcccaccc agatacgcga aaattacgcc gccgtgagta ttcaggccaa tgaatggaat
     7141 gtactcaccc agacgatcaa acaacgcata caacgcgacc agtgaaagta ctgccgggaa
     7201 catctggaaa atcagcattc ctttcagcag cgtcgctttg cctggaaagc gcatacgggc
     7261 gaaagcgtag gcgcaggtgg tggagagcgc cacaatgcca atcgcggaaa tcccagcgac
     7321 ctttaccgag ttccacagcc acagcagtac cgggaatggc ggtggcgtaa tgcgaccatc
     7381 agcctgttca acgctaaaac ctaacgccag tttccagtga tcccaggaga tttgctccgg
     7441 gatcaggcta ccggtcgcga agtttccctg acgcagcgag atagcgacga ccatcagcag
     7501 cgggaacata atcgccgcga taaaaagtag cagtagcagg tgagtaataa acaaacgtgc
     7561 tttttgcgat ttcggttgga ccattgccat ttttgttatc tcccttaatc aaacttcatt
     7621 cgcgtggctt tcaggttcac tatcgccagc gcacccacca gcaggaagat cagcgtggca
     7681 attgctgccg ccagaccgaa gtcctgaccc ccgccgcctt caaaagcgat acggtaggtg
     7741 tagttaacaa gcaggtcggt ataaccggct ggcgtggtcg tgccaagacg atccgggccg
     7801 ccgttggtta acagctgaat cagcacgaag ttgttaaagt taaaggcgaa gctggcgatc
     7861 atcagcggcg tcagcggttt aatcagcagc ggcagcgtaa tcttaaagaa gttctggaac
     7921 ggacctgcgc catccattgc tgaggcttca tacaaatcgt ccggaatcgc tttcagcaag
     7981 cccatgcaga ggatcatcat gtacggataa cccagccagg tattgacgat aatcagcatc
     8041 gtgcgagcgg tggtcggatc gctgaaccag gcgggcttca cgccaaacag cgcgctcaac
     8101 atcatgttga tttcaccgaa gctctggtta aacaatcctt tgaaaatcaa gattgaaatg
     8161 aacgatggca ccgcgtaggg cagaatcagc aggacgcgat agaccgcttt gccgcgcaac
     8221 gcttcccact gcaccagaca tgccagaacc atgccgaccg ccaccgttaa aaagacagtg
     8281 atcagcgaga acaccacggt ccagacgaaa atggcgagga acggtttctg aatgccttcg
     8341 tcggtaaaga cgcgggtaaa gtttttccag ccggtggtca cggtgtaacc ggggcttagc
     8401 ttttcatccc cccagttgcc gtcggcggta atggactggt aaaagccaat ttggttattc
     8461 ggacgatatt tcacgccact ctgattattc gtcaacgtgc cgtcaccgtc gagtgtgtag
     8521 agcggctgcg tgccagaaaa ctggcgcagg gagctcatca tcactttgtt gccatccggc
     8581 agaatggcgg taatgtcact cagcgcctga cgattctggg taatcacgcg cagattcgcg
     8641 cgttcgcctt cgggctgggc ggtcgtttct ttcagttgca gtttttgctc gccgccaaat
     8701 ttaaacgcgt cggagaggta atttttgccg gtttcgccgt cgctgagcgc cagttgccac
     8761 tcatcgcccg ccgggtaaag accaaagtta taggttttgc ctgcttgcca ggagcgatct
     8821 aacaacactt cctgcgcacg ttcaaaagtc agctggttag tgctgctgta gttggtgaag
     8881 gcaatggcga tggtgcagac cagagggaag aggacgaata atcccattcc agccattccc
     8941 gggtaaacat agcgccaggc gtaggcttta cgattggcga aaatatacag ccccgctgaa
     9001 ctcaatatca gcgtggtaat ggcgaacagg tattcccctt gtgcgtacat taaaacaaca
     9061 aggtaaccca ccagcaggcc gagcagacct agcactgacc atttcagcgc gtcgctttgc
     9121 caccaatgtt tctttttaat gacatccatg gggttcttcc tcattccagg acggataagg
     9181 ctttcacgcc ttatccgaca acaactgcct gatgcgacac tgatgcgtct tatcaggcct
     9241 acatgcgttt cagttttgta ggccggacaa ggcgttcacg ccgcatccgg catttcacag
     9301 cattacttgg tgatacgagt ctgcgcgtct ttcagggctt catcgacagt ctgacgaccg
     9361 ctggcggcgt tgatcaccgc agtacgtacg gcataccaga aagcggacat ctgcgggatg
     9421 ttcggcatga tttcaccttt ctgggcgttt tccatggtgg cggcaatacg tggatctttc
     9481 gccaactctt cctcgtaaga cttcagcgct acggcaccca gcggtttgtc tttattaacc
     9541 gcttccagac cttcatcagt cagcagatag ttttcgagga actctttcgc cagctctttg
     9601 ttcggactgg cggcgttaat accggtgctc agcacgccaa cgaacggttt agatggttga
     9661 cccttgaagg tcggcagtac cgttacacca taattcactt tgctggtgtc gatgttggac
     9721 catgcccacg ggccgttgat ggtcatcgct gtttcgcctt tattaaaggc agcttctgcg
     9781 atggagtaat cggtgtctgc attcatgtgt ttgtttttaa tcaggtcaac caggaaggtc
     9841 agacccgctt tcgcgccagc gttatccacg cccacgtctt taatgtcgta cttgccgttt
     9901 tcatacttga acgcataacc cccgtcagca gcaatcagcg gccaggtgaa gtacggttct
     9961 tgcaggttga acatcagcgc gctcttacct ttcgctttca gttctttatc cagcgccggg
    10021 atctcttccc aggtttttgg cgggttcggc agcaggtctt tgttataaat cagcgataac
    10081 gcttcaacag cgattgggta agcaatcagc ttgccgttgt aacgtacggc atcccatgta
    10141 aacggataca gcttgtcctg gaacgctttg tccggggtga tttcagccaa caggccagat
    10201 tgagcgtagc caccaaagcg gtcgtgcgcc cagaagataa tgtcagggcc atcgccagtt
    10261 gccgcaacct gagggaattt ctcttccagt ttatccggat gctcaacggt gactttaatt
    10321 ccggtatctt tctcgaattt cttaccgact tcagcgagac cgttatagcc tttatcgccg
    10381 ttaatccaga ttaccagttt accttcttcg attttggcga gagccgaggc ggaaaacatc
    10441 atcgtcgtta atgcggataa tgcgaggatg cgtgcacctg tttttatttt cataatctat
    10501 ggtccttgtt ggtgaagtgc tcgtgaaaac acctaaacgg actctagttt ctttatacgg
    10561 caacctcttt ccatcctcct tgcccctacg ccccaccgtt gctttgtgtg atctctgtta
    10621 cagaattggc ggtaatgtgg ggatgcgcac ataaaatcgc cacgattttt gcaagcaaca
    10681 tcacgaaatt ccttacatga cctcggttta gttcacagaa gccgcgttct catcctcccg
    10741 cctcctcccc cataaaaaag ccagggggtg gaggatttaa gccatctcct gatgacgcat
    10801 agtcagccca tcatgaatgt tgctgtcgat gacaggttgt tacaaaggga gaagggcatg
    10861 gcgagcgtac agctgcaaaa tgtaacgaaa gcctggggcg aggtcgtggt atcgaaagat
    10921 atcaatctcg atatccatga aggtgaattc gtggtgtttg tcggaccgtc tggctgcggt
    10981 aaatcgactt tactgcgcat gattgccggg cttgagacga tcaccagcgg cgacctgttc
    11041 atcggtgaga aacggatgaa tgacactccg ccagcagaac gcggcgttgg tatggtgttt
    11101 cagtcttacg cgctctatcc ccacctgtca gtagcagaaa acatgtcatt tggcctgaaa
    11161 ctggctggcg caaaaaaaga ggtgattaac caacgcgtca accaggtggc ggaagtgctg
    11221 caactggcgc atttgctgga tcgcaaaccg aaagcgctct ccggtggtca gcgtcagcgc
    11281 gtggcgattg gccgtacgct ggtggccgag ccaagcgtat ttttgctcga tgaaccgctc
    11341 tccaacctcg atgctgcact gcgtgtgcaa atgcgtatcg aaatctcccg tctgcataaa
    11401 cgcctgggcc gcacaatgat ttacgtcacc cacgatcagg tcgaagcgat gacgctggcc
    11461 gacaaaatcg tggtgctgga cgccggtcgc gtggcacagg ttgggaaacc gctggaactg
    11521 taccactatc cggcagaccg ttttgtcgcc ggatttatcg gttcgccaaa gatgaacttc
    11581 ctgccggtaa aagtgaccgc caccgcaatc gatcaagtgc aggtggagct gccgatgcca
    11641 aatcgtcaac aagtctggct gcctgttgaa agccgtgatg tccaggttgg agccaatatg
    11701 tcgctgggta ttcgcccgga acatctactg ccgagtgata tcgctgacgt catccttgag
    11761 ggtgaagttc aggtcgtcga gcaactcggc aacgaaaccc aaatctatat ccagatccct
    11821 tccattcgtc aaaacctggt gtaccgccag aacgacgtgg tgttggtaga agaaggtgcc
    11881 acattcgcta tcggcctgcc gccagagcgt tgccatctgt tccgtgagga tggcactgca
    11941 tgtcgtcgac tgcataagga gccgggcgtt taagcacccc acaaaacaca caaagcctgt
    12001 cacaggtgat gtgaaaaaag aaaagcaatg actcaggaga tagaatgatg attactctgc
    12061 gcaaacttcc tctggcggtt gccgtcgcag cgggcgtaat gtctgctcag gcaatggctg
    12121 ttgatttcca cggctatgca cgttccggta ttggctggac aggtagcggc ggtgaacaac
    12181 agtgtttcca gactaccggt gctcaaagta aataccgtct tggcaacgaa tgtgaaactt
    12241 atgctgaatt aaaattgggt caggaagtgt ggaaagaggg cgataagagc ttctatttcg
    12301 acactaacgt ggcctattcc gtcgcgcaac agaatgactg ggaagctacc gatccggcct
    12361 tccgtgaagc aaacgtgcag ggaaaaaacc tgatcgaatg gctgccaggt tccaccatct
    12421 gggcaggtaa gcgcttctac caacgtcatg acgttcatat gatcgacttc tactactggg
    12481 atatttctgg tcctggtgcc ggtctggaaa acatcgatgt tggcttcggt aaactctctc
    12541 tggcagcaac ccgctcctct gaagctggtg gttcttcctc ttttgccagc aacaatattt
    12601 atgactatac caacgaaacc gcgaacgacg ttttcgatgt gcgtttagcg cagatggaaa
    12661 tcaacccggg cggcacatta gaactgggtg tcgactacgg tcgtgccaac ctgcgtgata
    12721 actatcgtct ggttgatggc gcatcgaaag acggctggtt attcactgct gaacatactc
    12781 agagtgtcct gaagggcttt aacaagtttg ttgttcagta cgctactgac tcgatgacct
    12841 cgcagggtaa aggtctgtcg cagggttctg gcgtcgcgtt tgataacgaa aaatttgcct
    12901 acaatatcaa caacaacggt cacatgctgc gtatcctcga ccacggtgcg atctccatgg
    12961 gcgacaactg ggacatgatg tacgtgggta tgtaccagga tatcaactgg gataacgaca
    13021 acggcaccaa gtggtggacc gttggtattc gcccgatgta caagtggacg ccaatcatga
    13081 gcaccgtgat ggaaatcggc tacgacaacg tcgaatccca gcgcaccggc gacaagaaca
    13141 atcagtacaa aattaccctc gcacaacaat ggcaggctgg cgacagcatc tggtcacgcc
    13201 cggctattcg tgtcttcgca acctacgcca agtgggatga gaaatggggt tacgactaca
    13261 acggcgatag caaggttaac ccgaactacg gcaaagccgt tcctgctgat ttcaacggcg
    13321 gcagcttcgg tcgtggcgac agcgacgagt ggaccttcgg tgcccagatg gaaatctggt
    13381 ggtaatagca aaacctgggc cggataaggc gtttacgccg catccggcat aaaaacaggt
    13441 tgtcattatc tgaaaggggc gaaagcccct ctgattatcg ggtttagcgc gctattgcct
    13501 ggctaccgct gagctccaga ttttgaggtg aaaacaatga aaatgaataa aagtctcatc
    13561 gccctctgtt tatcagcagg gttactggca agcgcgcctg gaattagcct tgccgatgtt
    13621 aactacgtac cgcaaaacac cagcgacgcg ccagccattc catctgccgc gctgcaacaa
    13681 ctcacctgga caccggtcga tcaatctaaa acccagacca cccaactggc gaccggcggc
    13741 caacaactga acgttcccgg catcagtggt ccggttgctg cgtacagcgt cccggcaaac
    13801 attggcgaac tgaccctgac gctgaccagc gaagtgaaca aacaaaccag cgtttttgcg
    13861 ccgaacgtgc tgattcttga tcagaacatg accccatcag ccttcttccc cagcagttat
    13921 ttcacctacc aggaactagg cgtgatgagt gcagatcggc tggaaggcgt tatgcgcctg
    13981 acaccggcgt tggggcagca aaaactttat gttctggtct ttaccacgga aaaagatctc
    14041 cagcagacga cccaactgct cgacccggct aaagcctatg ccaaaggcgt cggtaactcg
    14101 atcccggata ttcccgatcc ggttgctcgt cataccagcg atggcttact gaaactgaaa
    14161 gtcaaaacga actccagctc cagcgtgttg gtaggaccgc tatttggttc ttccgctcca
    14221 gctccggtta cggtaggcaa cacggcggca ccagctgtgg ctgcacctgc tccggtgccg
    14281 gtgaagaaaa gcgagccgat gctcaacgac acggaaagtt attttaatac cgcgatcaaa
    14341 aacgctgtcg cgaaaggtga tgttgataag gcgttaaaac tgcttgatga agctgaacgc
    14401 ctgggatcga catctgcccg ttccaccttt atcagcagtg taaaaggcaa ggggtaatta
    14461 cgccccacag tgctgatttt gcaacaactg gtgcgtctcc tggcgcacct ttttttatgc
    14521 ttccttcctg ggatatgagc gattttttat agtacttcac ttcttcttca ctaagaatgt
    14581 ccattatttc aatgcattat cagagattct tttcctttcg ccggtagtgt ctggacgctc
    14641 aggctacttt tccaggttat tttatttctg tattacagag gttttatgat aggtcatatc
    14701 acaaattctg gcggcaataa ctctttgatg aaacatgatg tggtgcaagg aaataatata
    14761 gtagatcttg atttactacg taatttcaat ggggtgacag gtttaaacag agattacttt
    14821 atttatatca gtgatatatt tttaaatata aaacaacgga acgaaaaaaa tcatgcaata
    14881 aatatgttac gtgaagtctc aattagtaat gatactataa gtgtaaaatt ctacagaaat
    14941 gaaaaaatag aatgcgcttg taattttctg atggataaag atgcgcaggg gtatatcgac
    15001 ctgtctgatt ttgatttaac aaattgtcat tttaaaggtg acgttatttc taaggtgtct
    15061 tttttatcat caaatctaca acatgtaact ttcgaatgta aagaaattga gaattgcaat
    15121 tttactaagg caacagttga taatgtcata tttaaatgtc gacgtttaca caatgtgatt
    15181 tttctcaaaa cgagtggcga atgtgttgat tttagccaaa atattcttga tacagttgac
    15241 ttctcgcaga gtcaacttgg tcatagtaat tttcgcgaat gtcagattag aaattcaaac
    15301 ttcgataatt gttatcttta cgcttcgcac ttcaccagag cagagtttct gtctgccaaa
    15361 gaaatatcat ttattaaatc gaatttgaca gctgttatgt ttgatcatgt gcgaatgtcg
    15421 acagggaatt tttaagattg cattacagaa caattggaat taactattga ttattcagat
    15481 atatttggga atgaagatct cgatggttat atcaataaca ttataaaa
//
LOCUS       DAZXUK010000081        15410 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZXUK010000081 DAZXUK010000000
VERSION     DAZXUK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15410
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.081"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1175)
                     /locus_tag="ACH3L8_001971"
     CDS             complement(<1..1175)
                     /locus_tag="ACH3L8_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4085101.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1374..1458)
                     /locus_tag="ACH3L8_001972"
     tRNA            complement(1374..1458)
                     /locus_tag="ACH3L8_001972"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Leu,seq:caa)
     gene            1654..2673
                     /gene="ahr"
                     /locus_tag="ACH3L8_001973"
     CDS             1654..2673
                     /gene="ahr"
                     /locus_tag="ACH3L8_001973"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFT4085102.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2802..4304
                     /locus_tag="ACH3L8_001974"
     CDS             2802..4304
                     /locus_tag="ACH3L8_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFT4085103.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="ACH3L8_001975"
     CDS             complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="ACH3L8_001975"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFT4085104.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="ACH3L8_001976"
     CDS             complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="ACH3L8_001976"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFT4085105.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6668..6718)
                     /gene="ytgA"
                     /locus_tag="ACH3L8_001977"
     CDS             complement(6668..6718)
                     /gene="ytgA"
                     /locus_tag="ACH3L8_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFT4085106.1"
                     /translation="MSIIILATISTTQENK"
     gene            6872..8383
                     /gene="pepA"
                     /locus_tag="ACH3L8_001978"
     CDS             6872..8383
                     /gene="pepA"
                     /locus_tag="ACH3L8_001978"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFT4085107.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8517..8960
                     /gene="holC"
                     /locus_tag="ACH3L8_001979"
     CDS             8517..8960
                     /gene="holC"
                     /locus_tag="ACH3L8_001979"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFT4085108.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8960..11815
                     /gene="valS"
                     /locus_tag="ACH3L8_001980"
     CDS             8960..11815
                     /gene="valS"
                     /locus_tag="ACH3L8_001980"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFT4085109.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11869..13065)
                     /locus_tag="ACH3L8_001981"
     CDS             complement(11869..13065)
                     /locus_tag="ACH3L8_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFT4085110.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13258..13761
                     /locus_tag="ACH3L8_001982"
     CDS             13258..13761
                     /locus_tag="ACH3L8_001982"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFT4085111.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="ACH3L8_001983"
     CDS             complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="ACH3L8_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFT4085112.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14385..15398
                     /gene="argF"
                     /locus_tag="ACH3L8_001984"
     CDS             14385..15398
                     /gene="argF"
                     /locus_tag="ACH3L8_001984"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFT4085113.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3977 a         3932 c         3969 g         3532 t
ORIGIN      
        1 atcaacctac gctccccaag atgttccgcc ttatggatgt aagctgctcg cacagagcta
       61 cgctcctggt gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc
      121 aacgaactac aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac
      181 cccattacgc gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga
      241 tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt
      301 agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa
      361 tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa
      421 tctatttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg
      481 agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat
      541 tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca
      601 cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata
      661 atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact
      721 gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata
      781 gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac
      841 ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc
      901 tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta
      961 cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat
     1021 gccagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta
     1081 gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc
     1141 ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg
     1201 tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt
     1261 gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat
     1321 actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc
     1381 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1441 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1501 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1561 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1621 acttcccaaa caacaccaga gaaggcccca aaaatgtcga tgataaaaag ctatgccgca
     1681 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     1741 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     1801 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgta
     1861 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     1921 ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac
     1981 tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt
     2041 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     2101 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     2161 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcac
     2221 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2281 gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2341 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2401 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2461 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2521 ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc
     2581 gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg
     2641 cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc
     2701 acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca
     2761 gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa
     2821 ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc
     2881 tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat
     2941 tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg
     3001 cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg
     3061 actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca
     3121 cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca
     3181 acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt
     3241 tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat
     3301 ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat
     3361 tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca
     3421 aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga
     3481 aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt
     3541 atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg
     3601 aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga
     3661 taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata
     3721 ttccggataa tgtgttcggc cagctcggta atcgtgttca gcacgctttg cgggctttta
     3781 cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat
     3841 ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg
     3901 atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa
     3961 tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca
     4021 aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg
     4081 ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg
     4141 gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga
     4201 aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg
     4261 tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt
     4321 gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac
     4381 tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa
     4441 cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg
     4501 gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     4561 gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     4621 aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     4681 catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     4741 gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg
     4801 cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt
     4861 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     4921 cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact
     4981 gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc
     5041 tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
     5101 gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatcg ccatcgtcag
     5161 caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc
     5221 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
     5281 cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt
     5341 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
     5401 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
     5461 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
     5521 cgcgaaaaac tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc
     5581 gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct
     5641 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
     5701 gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc
     5761 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga
     5821 gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg
     5881 tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg
     5941 aaatcacgta acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg
     6001 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
     6061 ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt
     6121 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc
     6181 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
     6241 ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt
     6301 gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt
     6361 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
     6421 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
     6481 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
     6541 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
     6601 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
     6661 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
     6721 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
     6781 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
     6841 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
     6901 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
     6961 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg
     7021 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
     7081 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
     7141 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg
     7201 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
     7261 cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc
     7321 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
     7381 tgagcgcgcg atccagcacg gtcaggcgat tgccgccggg ataaaagcag cgaaagatct
     7441 cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct
     7501 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
     7561 gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc
     7621 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
     7681 aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat
     7741 gaagtacgat atgtgcggcg cggcggcagt ttacggcgtg atgcgtatgg tcgcggagct
     7801 gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg
     7861 tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa
     7921 caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga
     7981 gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
     8041 tcatattact ggtctgatga cgaaccataa tccgctggcc catgaactga ttgccgcgtc
     8101 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
     8161 ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc
     8221 aggttgcttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg
     8281 aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc
     8341 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc
     8401 accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc
     8461 atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga
     8521 aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg
     8581 agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg
     8641 cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag
     8701 aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcagtgcg ccggtggaga
     8761 tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa
     8821 gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt
     8881 ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga
     8941 atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct
     9001 ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag
     9061 tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt
     9121 ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct
     9181 gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat
     9241 tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat
     9301 ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa
     9361 ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa
     9421 agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa
     9481 ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa
     9541 aggttcgatg tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa
     9601 agattatctg gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc
     9661 tgttaacccg gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct
     9721 ggttaaccgt cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg
     9781 ctgcgtgaag atcacaccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc
     9841 cctgccgatg atcaacatcc tgacctttga cggtgatatc cgtgaaagcg cccaggtgtt
     9901 cgataccaaa ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa
     9961 actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct
    10021 ggaagaaatt aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat
    10081 cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt
    10141 tgaagcggtt gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt
    10201 ctcctggatg cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg
    10261 tatcccggca tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt
    10321 gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga
    10381 cacctggttc tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga
    10441 cgccctgcgt cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt
    10501 ctggattgcc cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc
    10561 gcaggtgccg ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa
    10621 gatgtccaaa tccaagggta acgttattga cccgctggat atggttgacg gtatttcgct
    10681 gccagaactg ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat
    10741 ccgtaagcgc accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct
    10801 gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg
    10861 tctggaaggc taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat
    10921 gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga
    10981 ccgctggatt ctggcggagt tcaaccagac tatcaaagcg taccgcgaag cgctggacag
    11041 cttccgcttc gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga
    11101 ctggtatctg gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg
    11161 tactcgccat acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat
    11221 tccgttcatc accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga
    11281 caccatcatg ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact
    11341 ggccgacacc gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat
    11401 gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga
    11461 acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac
    11521 cgtgctgcct gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga
    11581 gctgctgatc ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa
    11641 agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg
    11701 ctttgtcgcc cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta
    11761 tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac
    11821 acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg
    11881 taaagaaggc ataactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc
    11941 tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca
    12001 gtcaatcatc catattttca gcagtgggta agccagacca cccgtaatcc cggatatcac
    12061 caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat
    12121 acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata
    12181 actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg
    12241 catttccata aattgctgta aattctcaat atcatcattt gcatacccag aactatcata
    12301 ctcatttaat atttgatcga agataatata accagcaaca gcgagaaaag gtaataaagc
    12361 taaaaatgcg aggatggcat attttataca atacgcagta tctaatttca ccttgaaacg
    12421 atgtataccg aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc
    12481 attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag atataataac
    12541 actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc
    12601 aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt
    12661 aaaactaaat cttacgccgt tgagactggt cattaaggcc tgataacgca gtccttttgc
    12721 tgccataaat ataagcaaaa ccaacagtaa caaagtcaaa acacagccaa caagcggcat
    12781 atctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa gacaactaac
    12841 aaaaacattc ccaccggtaa ttccataaga aaatcgttgt ccgttaactt ccatattagc
    12901 ataaagataa cgcttacatt tcattaatgc ccatggtaaa tagataccta gcgttataat
    12961 cgttaacaac acattcacca cacaaataag aaaatatctc tcacctgtac catgaaaaac
    13021 aaaagaatgg gacggaacat ccatttcatt aataacttga gccataaaaa tcccctgaat
    13081 taaaaaatcc attgatataa tttatagata cagacagtaa aatgttctgc cacgttacaa
    13141 tactcttatc agtaataggc tggcaaaaac tttattgctt tataagacgc ttgcgcacgt
    13201 aagaagagag tggtattaaa caaatgtata attactttat ttttattggg ttaaactatg
    13261 aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga taatcctgct
    13321 atcgccagcg tcatccgtca ggtttacgca gagtatggtc ttaccgccga taaaggctac
    13381 accgttgccg atccgaatct cgacgaactg tatcaagtgt acagccagcc tggccatgct
    13441 tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg gcgggattgc gccattaaca
    13501 ggtagtgaag cggacatttg cgaactgcag aaaatgtact ttcttcccgc tattcgcggt
    13561 aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga gatgggcttc
    13621 aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat
    13681 ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga ttgtgaagtg
    13741 cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg cattttaatc
    13801 aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccatc
    13861 ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt caaatttctc
    13921 cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc
    13981 gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc
    14041 ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag tttccagatc
    14101 gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag
    14161 taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt ccgggtttgc
    14221 catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc actgacattg
    14281 cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat aatacacata
    14341 aattgagttt taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc
    14401 ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt
    14461 tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga agaagccaaa ctcactggta
    14521 aaaacatcgc gctcatcttc gaaaaagact cgactcgtac ccgatgctct ttcgaagttg
    14581 ccgcatatga ccagggggct cgcgttactt atcttggccc aagcggtagt cagattggtc
    14641 ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac ggtattcagt
    14701 atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc gtgccggtat
    14761 ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc
    14821 aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac
    14881 gtaacaacat gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttgcgtc
    14941 tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt tacggaatgc cgcgccctgg
    15001 cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga gttgaaggtg
    15061 ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagaa aaatgggctg
    15121 aacgcattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta
    15181 acccggaggt caaattcctc cattgcctgc ccgcgtttca tgacgaccaa acgacgcttg
    15241 gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact gatgaggtct
    15301 tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat accatcaaag
    15361 cggtgatggt cgcgacgctc agtaaattaa ataattaata tcgctataaa
//
LOCUS       DAZXUK010000082        15408 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZXUK010000082 DAZXUK010000000
VERSION     DAZXUK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15408
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.082"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..741
                     /gene="cysD"
                     /locus_tag="ACH3L8_001985"
     CDS             <1..741
                     /gene="cysD"
                     /locus_tag="ACH3L8_001985"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFT4085114.1"
                     /translation="PFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGI
                     NPFVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRW
                     DPKNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPV
                     LERDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLV
                     STTSERQGRVIDRDQAGSMELKKRQGYF"
     gene            743..2170
                     /gene="cysN"
                     /locus_tag="ACH3L8_001986"
     CDS             743..2170
                     /gene="cysN"
                     /locus_tag="ACH3L8_001986"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFT4085115.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            2170..2775
                     /gene="cysC"
                     /locus_tag="ACH3L8_001987"
     CDS             2170..2775
                     /gene="cysC"
                     /locus_tag="ACH3L8_001987"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFT4085116.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            2825..3148
                     /locus_tag="ACH3L8_001988"
     CDS             2825..3148
                     /locus_tag="ACH3L8_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFT4085117.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            3342..3653
                     /gene="ftsB"
                     /locus_tag="ACH3L8_001989"
     CDS             3342..3653
                     /gene="ftsB"
                     /locus_tag="ACH3L8_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFT4085118.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            3672..4382
                     /gene="ispD"
                     /locus_tag="ACH3L8_001990"
     CDS             3672..4382
                     /gene="ispD"
                     /locus_tag="ACH3L8_001990"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFT4085119.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            4382..4861
                     /gene="ispF"
                     /locus_tag="ACH3L8_001991"
     CDS             4382..4861
                     /gene="ispF"
                     /locus_tag="ACH3L8_001991"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFT4085120.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            4858..5907
                     /gene="truD"
                     /locus_tag="ACH3L8_001992"
     CDS             4858..5907
                     /gene="truD"
                     /locus_tag="ACH3L8_001992"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFT4085121.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            5888..6649
                     /gene="surE"
                     /locus_tag="ACH3L8_001993"
     CDS             5888..6649
                     /gene="surE"
                     /locus_tag="ACH3L8_001993"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFT4085122.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            6643..7269
                     /gene="pcm"
                     /locus_tag="ACH3L8_001994"
     CDS             6643..7269
                     /gene="pcm"
                     /locus_tag="ACH3L8_001994"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFT4085123.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            7454..8548
                     /gene="nlpD"
                     /locus_tag="ACH3L8_001995"
     CDS             7454..8548
                     /gene="nlpD"
                     /locus_tag="ACH3L8_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFT4085124.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            8611..9603
                     /gene="rpoS"
                     /locus_tag="ACH3L8_001996"
     CDS             8611..9603
                     /gene="rpoS"
                     /locus_tag="ACH3L8_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFT4085125.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(9723..10130)
                     /locus_tag="ACH3L8_001997"
     CDS             complement(9723..10130)
                     /locus_tag="ACH3L8_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFT4085126.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            10277..10870
                     /locus_tag="ACH3L8_001998"
     CDS             10277..10870
                     /locus_tag="ACH3L8_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFT4085127.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            10870..11817
                     /locus_tag="ACH3L8_001999"
                     /pseudo
     CDS             10870..11817
                     /locus_tag="ACH3L8_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(12090..12653)
                     /locus_tag="ACH3L8_002000"
     CDS             complement(12090..12653)
                     /locus_tag="ACH3L8_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085128.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            12818..13069
                     /locus_tag="ACH3L8_002001"
     CDS             12818..13069
                     /locus_tag="ACH3L8_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFT4085129.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            13071..15167
                     /locus_tag="ACH3L8_002002"
     CDS             13071..15167
                     /locus_tag="ACH3L8_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFT4085130.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         3799 a         3757 c         4227 g         3625 t
ORIGIN      
        1 cctttcccgt tgctgcatgt cgataccggc tggaaattcc gcgagatgta tgagttccgc
       61 gatcgtacgg cgaaagccta cggctgcgag ctgctggtgc ataaaaaccc ggaaggcgtg
      121 gcgatgggga ttaatccatt cgtgcacggc agcgcgaaac ataccgatat tatgaaaact
      181 gaaggcctga aacaggcgct gaacaaatac ggttttgatg ccgccttcgg tggtgcgcgc
      241 cgtgacgaag agaaatcccg cgctaaagag cgaatctact ctttccgtga ccgcttccat
      301 cgctgggatc cgaaaaatca gcgcccggag ctgtggcaca actacaacgg gcaaattaac
      361 aaaggcgaaa gcatccgcgt cttcccgctc tctaactgga ccgagcagga tatctggcaa
      421 tacatctggc tggaaaatat cgacattgtg ccgctgtatc tcgctgcgga acgtccggtt
      481 ctggaacgcg acggtatgtt gatgatgatt gatgacaacc gtatcgacct gcaaccgggc
      541 gaagtgatca aaaaacggat ggtgcgtttc cgtacgctgg gctgctggcc gctgaccggt
      601 gcggtggagt caaatgcaca aacactgccg gaaatcatcg aagagatgct ggtttccacc
      661 accagtgaac gtcagggtcg cgtgattgac cgcgaccagg cgggatccat ggagctgaaa
      721 aaacgtcagg ggtattttta agatgaacac cgtacttgca caacaaatcg ccaatgaagg
      781 cggcgtcgaa gcctggatga ttgcgcaaca acataaaagc ctgctgcgtt ttctgacctg
      841 tggtagcgtc gatgacggca aaagtaccct gattggtcgt ctgctgcacg atacccgcca
      901 aatctacgaa gatcagctct catcgctgca taacgacagt aagcgtcacg gcacccaggg
      961 cgaaaagctg gatctggctc tgctggtgga cggcctgcaa gctgagcgcg aacagggcat
     1021 cactattgat gtggcctacc gctatttctc taccgagaag cgtaaattta ttatcgccga
     1081 caccccaggg cacgagcagt acactcgcaa tatggcgact ggcgcatcga catgtgaact
     1141 ggcgatctta ctgattgatg cccgtaaagg cgtgctcgat caaacccgtc gtcacagttt
     1201 tatctccaca ctgttgggga tcaaacatct ggtcgtggcg atcaacaaaa tggatctggt
     1261 ggattacagt gaagagacgt tcacccgtat tcgtgaagat tatctgacct ttgccgggca
     1321 gctgccgggt aatctggata tccgctttgt gccgctctcc gcactggaag gtgacaacgt
     1381 ggcttcgcaa agtgaaagta tgccgtggta cagcggtctg acactgctcg aagtgctgga
     1441 aaccgtggag atccagcgag tggtggatgc ccagccaatg cgcttcccgg tgcagtacgt
     1501 taaccgccca aatctcgatt ttcgtggcta cgccggaatg ctggcatccg gtcgcgtgga
     1561 agtcgggcaa cgtgtaaaag tgctgccctc tggtgtggaa tcaaacgtcg cgcggatcgt
     1621 gacttttgat ggtgatcgcg aagaagtctt tgccggagaa gcgatcaccc tggtgctgac
     1681 ggatgagatc gatatcagcc gtggtgatct gctgctggcg gcagacgaag cgttaccggc
     1741 ggtgcagagt gcgtcggtgg atgtggtatg gatggcggaa cagccgcttt ccccgggcca
     1801 gagttatgac atcaaaattg ccggtaagaa gacgcgtgct cgtgttgatg gcattcgtta
     1861 tcaggttgat attaataacc ttacccaacg cgaagttgaa aacctgccgc tgaacggcat
     1921 cggcctggtg gatctcactt ttgacgagcc actggtgtta gatcgctatc agcaaaatcc
     1981 ggttaccggt gggctgattt ttatcgatcg cctgagcaat gtgaccgtgg gtgccggtat
     2041 ggtgcacgag ccagttagcc aggcaacagc tgcgtcatct gaattcagtg cattcgaact
     2101 ggaattgaat gccctggttc gtcgccactt tccgcactgg ggcgcgcgcg atttgctggg
     2161 ggataaataa tggcgctgca tgacgaaaac gtcgtctggc atagtcatcc ggtcactgtg
     2221 caacaacgcg aactacacca cggtcatcgt ggtgtagtgc tgtggtttac cggcctctcc
     2281 gggtccggta aatcaacggt cgccggggcg ctggaggagg cgttacataa actcagcgtc
     2341 agtacgtatc tgctggatgg cgacaatgtt cgccacggat tatgcagcga tctcggtttt
     2401 agcgatgccg atcgtaaaga gaatatccgt cgcgtcggtg aagtggcgaa tttgatggtt
     2461 gaagccggac tggtggtgct gaccgcattt atctcgccac accgcgccga acgccagatg
     2521 gttcgcgaac gcgtaggaga agggcgcttt attgaagtgt ttgtcgatac gccgctggcg
     2581 atttgcgaag cccgcgatcc aaaaggctta tataagaaag cgcgtgccgg tgaactgcgc
     2641 aactttacgg gaatagattc cgtttacgaa gcgcctgaat cggcagaaat tcatctcaat
     2701 ggtgaacaat tagtaacaaa tttggtacag caattattag atctgttgag acagaacgat
     2761 attatcagat cctgagacac caccgggttt ccatgcccgg ttcgtcaaag taacaggatc
     2821 aaatatgcgt aatagccata acattacact aacaaataac gacagcctta ctgaggatga
     2881 agaaactaca tggtcactgc ctggtgccgt ggtcggtttt atctcctggt tatttgcgct
     2941 ggcgatgccg atgttgattt atggctctaa cacgctgttc ttctttctct acacctggcc
     3001 tttctttctg gcactgatgc ctgtcgcggt agtggtgggg attgcgctgc attcattgat
     3061 ggacggaaag ctacgctaca gtattgtttt caccctggtg acagtaggca ttatgtttgg
     3121 cgcactgttt atgtggctac tgggctaatt tgtactttct tgtctcctct gttcataatt
     3181 caaaccgtaa ctaataatga gattatgttc tgcacgccct gggtatacgt aacaatgggc
     3241 aaatgtggta catttgcccg cgttgtcgcg gtatccccaa cagaggatgt agagtcgtct
     3301 tcggatgcat gggatgatga tgcagttttt cagggggcag gatgggtaaa ctaacgctgc
     3361 tgttgctggc tattctggtc tggctacagt attcgctgtg gttcggtaag aacggtatac
     3421 atgactatac ccgcgtcaat gatgatgtgg cggcactgca agctacaaac gcgaaactta
     3481 aagcgcgaaa cgatcaactt tttgccgaaa ttgacgatct caatggcggc caggaggcgc
     3541 tcgaagagcg tgcgcgtaat gaactcagca tgaccaggcc gggcgaaact ttttatcgtc
     3601 tggtgcctga cgcgtcgaag cgcgcacagt ctgcggggca aaacaatcga taaatcagcc
     3661 cgggaattaa catggcaacc actcatttgg atgtttgcgc cgtggttccg gcggccggat
     3721 ttggccgtcg aatgcaaacg gaatgtccta agcaatatct ctcaatcggt aatcaaacca
     3781 ttcttgaaca ctcggtgcat gcgctgctgg cgcatccccg ggtgacacgt gtcgtcattg
     3841 ccataagtcc tggcgatagc cgttttgcac aacttcctct ggcgaatcat ccgcaaatca
     3901 ccgttgtaga tggcggtgat gagcgtgccg attccgtgct ggcaggtctg aaagccgctg
     3961 gcgatgtgca gtgggtattg gtgcatgacg ccgctcgtcc ttgtttgcat caggatgacc
     4021 ttgcgcgatt gttggcgttg agcgaaacca gccgcacggg agggatcctc gccgcaccag
     4081 tgcgcgatac tatgaaacgt gccgaaccgg gcaaaaatgc cattgctcat accgttgatc
     4141 gcaacggctt atggcacgcg ctgacgccgc aatttttccc tcgtgagctg ttacatgact
     4201 gtctgacgcg cgctctaaat gaaggcgcta ccattaccga cgaagcctcg gcgctggaat
     4261 attgcggatt tcatccgcag ttggtcgaag gccgcgcgga taacattaaa gtcacgcgcc
     4321 cggaagattt ggcactggcc gagttttacc tcacccgaac catccatcag gagaatacat
     4381 aatgcgaatt ggacacggtt ttgacgtaca cgcctttggc ggtgaaggcc caattatcat
     4441 tggtggcgta cgcattcctt acgaaaaagg attgctggcg cattctgatg gcgacgtggc
     4501 gcttcatgcg ttgaccgatg cattgcttgg cgcggcggcg ctgggggata tcggcaaact
     4561 gttcccggat accgatccgg catttaaagg tgccgacagc cgcgagctgc tacgcgaagc
     4621 ctggcgtcgt attcaggcga agggttatac ccttggcaac gtcgatgtca ctatcatcgc
     4681 tcaggcaccg aagatgttgc cgcacattcc acaaatgcgc gtgtttattg ccgaagatct
     4741 cggctgccat atggatgatg ttaacgtgaa agccactact acggaaaaac ttggatttac
     4801 cggacgtggg gaagggattg cctgtgaagc ggtggcgcta ctcattaagg caacaaaatg
     4861 attgagtttg ataatctcac ttacctccac ggtaaaccgc aaggcaccgg gctgctgaaa
     4921 gccaatccgg aagactttgt ggtggtggaa gatttgggct ttgagcctga tggtgaaggt
     4981 gagcatattc tggttagaat cctcaaaaac ggctgcaata cccgttttgt ggcggatgca
     5041 ctggcgaaat tcctgaaaat tcattcccgt gaagtcagct tcgctgggca aaaagacaaa
     5101 catgctgtaa cggaacagtg gttatgtgct cgcgtgccgg gcaaggaaat gcccgatctg
     5161 agcgcctttc aactggaagg ctgccaggtg ctggagtatg cgcggcacaa gcgcaagctg
     5221 cgtttagggg cgctgaaagg taacgccttt accctggttc tgcgcgaagt gagcaatcgc
     5281 gatgacgttg aacaacgtct gatcgatatt tgcgtaaaag gtgtaccgaa ctacttcggt
     5341 gcccaacgtt ttgggattgg cggtagcaac ttgcagggcg cgctgcgctg ggcgcaaacc
     5401 aatactccgg tgcgcgatcg caataaacgg agtttttggt tgtcggcagc ccgcagtgcg
     5461 ttgtttaatc agattgttgc tgagcgcctc aaaaaagcag acgttaatca agttgttgac
     5521 ggcgatgcgc tacaattagc cggacgtggt agctggtttg tagcaaccac cgaagaactg
     5581 gcggaattac agcgtcgcgt caacgataaa gagctgatga taaccgccgc attgccgggc
     5641 agtggcgaat ggggaactca gcgtgaagcg ctggcattcg aacaagcagc tgtcgccgca
     5701 gaaactgaat tacaagcttt actggtgcgc gaaaaagttg aagccgcgcg cagagcgatg
     5761 ctgctgtatc cgcaacaatt aagctggaac tggtgggatg acgtcaccgt agagatccgt
     5821 ttctggcttc cggcgggtag ttttgcaacc agcgttgtca gggaacttat caacacaaca
     5881 ggtgattatg cgcatattgc tgagtaatta tgacggggta catgcacccg gtatacaaac
     5941 gctggcgaaa gccttgcgtg agtttgctga cgttcaggtg gtcgcccccg atcgtaaccg
     6001 cagcggcgct tcaaattctc tgacactgga atcctccctg cgcacgttta cctttgaaaa
     6061 tggtgatatt gctgtgcaaa tgggaacccc gaccgattgt gtctatcttg gcgtgaatgc
     6121 tctgatgcgt ccgcgcccgg acattgttgt gtccggaatt aacgccgggc cgaatctggg
     6181 ggatgatgtt atttattccg gtacggtagc cgccgcgatg gaaggccgtc atttaggttt
     6241 tccggcgctg gccgtctcgc ttgacgggca taaacattac gacactgccg cggcggtaac
     6301 ctgttcaatt ttgcgcgcac tgtgtaaaga gccgctgcgc accggacgta ttcttaatat
     6361 taacgttcag gatttaccct tggatcaaat caaaggtatt cgcgtgacgc gctgcggtac
     6421 acgacatccg gcagatcagg tgatcccgca gcaagatccg cgcggcaata cgctgtactg
     6481 gattggcccg ccgggcggta aatgtgatgc cggtccgggg accgattttg ctgcggtaga
     6541 tgagggctat gtctccatca cgccgctgca tgtggattta actgcgcata gcgcgcaaga
     6601 tgtggtttca gactggttaa acagcgtggg agttggcacg caatggtaag cagacgcgta
     6661 caagcacttc tggatcaatt acgtgcgcaa ggtattcagg atgagcaggt gctgaatgca
     6721 cttgccgccg tgccgcgtga aaaattcgtt gatgaagcgt ttgaacaaaa agcctgggac
     6781 aatatcgcct tgccgatagg tcaggggcag acaatttcgc agccgtatat ggtggcgcga
     6841 atgaccgaat tactcgagct gacgccgcag tcgcgggtgc tggaaattgg cactggttcg
     6901 ggatatcaaa cggcaatcct ggcgcatctt gtccagcatg tttgctcggt tgaacggatt
     6961 aaaggcttgc agtggcaggc acgtcgccgc ctgaaaaatc ttgatttaca taatgtttca
     7021 acccgtcatg gtgatggatg gcaaggttgg caggcacgtg cgccgtttga cgctatcatt
     7081 gttacggcgg caccgccgga aattccaact gcgctaatga cgcagctgga cgaaggcggg
     7141 attctcgtct tacccgtagg ggaggagcac cagtatttga aacgggtgcg tcgtcgggga
     7201 ggcgaattta ttatcgatac cgtggaggcc gtgcgctttg tccctttagt gaagggtgag
     7261 ctggcttaaa acgtgaggaa atacctggat ttttcctggt tattttgccg taggtcagcg
     7321 tatcgtgaac atcttttcca gtgttcagta gggtgccttg cacggtaatt atgtcactgg
     7381 ttattaacca atttttcctg ggggataaat gagcgcggga agcccaaaat tcaccgttcg
     7441 ccgcattgcg gctttgtcac tggtttcgct atggctggca ggctgttctg acacttcaaa
     7501 tccaccggca ccggtcagct ccgttaatgg caatgcgcct gcaaatacta attctggtat
     7561 gttgattacg ccgccgccga aaatggggac gacgtctaca gcgcagcaac cgcaaattca
     7621 gccggtacag cagccacaaa ttcaggctac tcaacaaccg caaatccagc cagtgcagcc
     7681 agtagctcag cagccggtac agatggaaaa cggacgcatc gtctataacc gtcagtatgg
     7741 gaacattccg aaaggcagtt atagcggcag tacctatacc gtgaaaaaag gcgacacact
     7801 tttctatatc gcctggatta ctggcaacga tttccgtgac cttgctcagc gcaacaatat
     7861 tcaggcacca tacgcgctga acgttggtca gaccttgcag gtgggtaatg cttccggtac
     7921 gccaatcact ggcggaaatg ccattaccca ggccgacgca gcagagcaag gagttgtgat
     7981 caagcctgca caaaattcca ccgttgctgt tgcgtcgcaa ccgacaatta cgtattctga
     8041 gtcttcgggt gaacagagtg ctaacaaaat gttgccgaac aacaagccaa ctgcgaccac
     8101 ggtcacagcg cctgtaacgg taccaacagc aagcacaacc gagccgactg tcagcagtac
     8161 atcaaccagt acgcctatct ccacctggcg ctggccgact gagggcaaag tgatcgaaac
     8221 ctttggcgct tctgaggggg gcaacaaggg gattgatatc gcaggcagca aaggacaggc
     8281 aattatcgcg accgcagatg gccgcgttgt ttatgctggt aacgcgctgc gcggctacgg
     8341 taatctgatt atcatcaaac ataatgatga ttacctgagt gcctacgccc ataacgacac
     8401 aatgctggtc cgggaacaac aagaagtgaa ggcggggcaa aaaatagcaa ccatgggtag
     8461 caccggaacc agttcaacac gcttgcattt tgaaattcgt tacaagggga aatccgtaaa
     8521 cccgctgcgt tatttgccgc agcgataaat cggcggaacc aggcttttgc ttgaatgttc
     8581 cgtcaaggga tcacgggtag gagccacctt atgagtcaga atacgctgaa agttcatgat
     8641 ttaaatgaag atgcggaatt tgatgagaac ggagttgagg tttttgacga aaaggcctta
     8701 gtagaagagg aacccagtga taacgatttg gccgaagagg aactgttatc gcagggagcc
     8761 acacagcgtg tgttggacgc gactcagctt taccttggtg agattggtta ttcaccactg
     8821 ttaacggccg aagaagaagt ttattttgcg cgtcgcgcac tgcgtggaga tgtcgcctct
     8881 cgccgccgga tgatcgagag taacttgcgt ctggtggtaa aaattgcccg ccgttatggc
     8941 aatcgtggtc tggcgttgct ggaccttatc gaagagggca acttggggct gatccgcgcg
     9001 gtagagaagt ttgacccgga acgtggtttc cgcttctcaa catacgcaac ctggtggatt
     9061 cgccagacga ttgaacgggc gattatgaac caaacccgta ctattcgttt gccgattcac
     9121 atcgtaaagg agctgaacgt ttacctgcga accgcacgtg agttgtccca taagctggac
     9181 catgaaccaa gtgcggaaga gatcgcagag caactggata agccagttga tgacgtcagc
     9241 cgtatgcttc gtcttaacga gcgcattacc tcggtagaca ccccgctggg tggtgattcc
     9301 gaaaaagcgt tgctggacat cctggccgat gaaaaagaga acggtccgga agataccacg
     9361 caagatgacg atatgaagca gagcatcgtc aaatggctgt tcgagctgaa cgccaaacag
     9421 cgtgaagtac tggcacgtcg attcggtttg ctggggtatg aagcggcaac actggaagat
     9481 gtaggtcgtg aaattggcct cacccgtgaa cgtgttcgcc agattcaggt tgaaggcctg
     9541 cgccgtttgc gcgaaatcct gcaaacgcag gggctgaata tcgaagcgct gttccgcaag
     9601 taagtaagca tctgtcagaa aggccagtct caagcgaggc tggccttttt cttttgtttg
     9661 ggaagcttgt gtttttgtcg cctggataag acacgtcagc gtcctacccg gcaatcagca
     9721 ttctatgtgc tcatcatctt gcgcaccaac tgcataaaca gctcctgctc ttctccactc
     9781 aaacgcccca aaaattcctc atccacgctg tcaccaatag gtatcgccgc agcaagtagc
     9841 ttttctcctt cggcagtcag ccagacaaag cgtcgccgct tgtcagcgac gtcatgttca
     9901 cgtctgacta agccacgatt ttccattctt gccaacattt ctgctagcgt tgctttggtg
     9961 ctgactgctg cttctatcag tgccacctgt tcaataccag gtttatcagc aatggcgcgc
    10021 atcaccgcgt actgtggttt ggtcaggtca ggtaactcgt gctgccagcg agccgtgtgc
    10081 tgctgaaaaa gctgtcgtaa ctgatggaac gctttatttc gtaacgccat gtaaactccg
    10141 ggctaattct ctgtcgctat catagcggtt ttttagtgac gaagaagatg ttgttacttt
    10201 tcaatccttg ccgctgtgga aatgaaggag tatgttaata ataacgttcg catacgaata
    10261 attaagagaa taagcaatga aactgatcgt cgggatgaca ggggctaccg gtgcgcctct
    10321 tggtgtggca ttactgcaag cgctgcgtga gatgccgaat gtcgagactc atctggtgat
    10381 gtcgaagtgg gcgaaaacca ccattgaact ggaaacgcct tacagcgctc gcgatgttgc
    10441 tgccctcgca gacttcagcc ataacccggc ggatcaggcg gcgatcatct catccggttc
    10501 ttttcgtacc gacggcatga tcgttattcc gtgcagtatg aaaacgctcg ccggtatccg
    10561 cgctggttac gccgatggcc tggtagggcg cgcggcggac gtcgtgctca aagaaggccg
    10621 caaactggtg ctggtgccgc gtgaaatgcc gcttagcacc atccatctcg aaaatatgct
    10681 cgcactttca cgcatgggcg tggcgatggt gccgccgatg cctgcctttt ataaccatcc
    10741 cgaaacggta gatgacattg tccaccatgt ggtagcccgc gtgctggatc aatttggcct
    10801 cgaacatccc cacgccaggc gctggcaagg attgccgcag gcccggaatt tttctcagga
    10861 gaatgaataa tggcatttga tgatttacgc agctttttac aggcgcttga tgaccacggc
    10921 cagttactga aaatcagcga agaagtgaac gctgagccgg atctggcagc agcagctaac
    10981 gccaccgggc gtatcggcga cggcgcgccc gcgctgtggt ttgataatat tcgcggcttt
    11041 accgatgccc gcgtggcgat gaataccatc ggttcctggc agaaccacgc gatttccctt
    11101 ggcctgccac caaacacccc ggtcaaaaag cagattgatg agtttatccg ccgctgggat
    11161 aacttcccga ttgccccgga gcgccgcgcc aatccagcct gggcgcagaa caccgtcgat
    11221 ggcgaagaga tcaacctgtt cgatattctg ccgctgtttc gcctgaatga tggcgatggc
    11281 ggtttctatc tcgacaaagc gtgcgtggtt tcccgcgatc cgctcgaccc ggataacttc
    11341 ggcaagcaga acgtcggcat ctaccgcatg gaagtgaagg gcaagcgtaa gctcggcctg
    11401 caaccggtgc cgatgcacga tatcgccctg catctgcata aagcagaaga gcgcggtgaa
    11461 gatctgccga ttgcgatcac gctcggtaac gatccgatca tcacgctgat gggggccacg
    11521 ccgctgaaat acgatcagtc tgagtatgaa atggcaggcg cgctgcgtga aagcccgtac
    11581 ccgatcgcca ccgctccgct gaccggtttt gatgtgccgt ggggttcaga agtgatcctc
    11641 gaaggggtta tcgaaagccg taaacgcgaa atcgaagggc cgttcggtga gtttaccggg
    11701 cactactccg gtgggcgcaa catgaccgtg gtgcgcatcg ataaagtctc ttatcgcagc
    11761 aaaccgattt tcgaatcact ctatctcggt atgccgtgga ccgaaatcga ctacctatga
    11821 ctaggtaatt tattcaagat aaccgggaag agattaaaaa agacgggaat agattggaat
    11881 caacaagtta ggaactcaag gttttcaaga taaagcgagt ccaaaaatgt taaacggttc
    11941 gatatcaata aaacattcaa gataactcac aggttcttac aatagccttt aaagaccgtt
    12001 caaaaacgat tcaggtgttt attatagcgc tgatgggtgc aagcgccaaa accaatgtaa
    12061 ttttagcaca gctaggcact tgcactgggt tatgcgctgg ctgctttctg aggtagattt
    12121 aagtgatcgc ccttagcttc ggacaacgcg taaatctctc taaccttttc gtctggcaaa
    12181 ttaccaaggc ttaacgctat ggctctacga actccaacta gagcgtgcaa ctcttggatg
    12241 tgctcatcca gaagaatgag taattgctcc gctccttttc tcccaagaac atcagctact
    12301 tgttgttttt cttgtggttt aagaaatgac aagaacatta gaatttgagc atcaacatta
    12361 tccgcattct cttttttggg ttctttggaa tcttttagtt caacatcacc ctctcccgtc
    12421 atgagccatt ccaaggtaac attttcgctt tctgctatct tttttaactg atacgggcgt
    12481 ggtatcggtg cgtaatcccg cctgttgtaa taatttttta aagtgccttc attgatcccc
    12541 catgcccgag cagccgcatt tcttgaaggg tatcgttcta ctaacctaaa gattctttcg
    12601 ataatcggtt cttttccatc ggcaggaaaa gaaccttggg gtgcttttct cattcttggt
    12661 tcctttctat ctaattcatt gataaataat aaaattaaaa aataaaccat aaaaacacca
    12721 aagaaccctt tgcatggaat ctttggtatc ttaatcttta tccatacgga tatccgaccg
    12781 gatatccgcg cgggtaattt tgtaggttaa aggaaagatg agacaaagag aagatagtga
    12841 ccgcgattgg ccttcgctga agattcagct ggcgctcaaa gataacgggt ggtcttcctt
    12901 ccgggagctt tcactccatg ctggttatgc tcaggattca ctgaaagcgg tagttcgcac
    12961 accaaacaaa aaatacgaac gcgtaatttc tgacgctata ggtgtaccgc cagaaatcat
    13021 ctggcccagc cggtatcgta acgatggatt tatcaggaag gcgtcgtgat atgcagtcgt
    13081 acgttaccgt aaatgacctg ctcggcgtac caggaatgcc agccaccaca aaaggcatcc
    13141 gacaggcgtt acagcgtttt tctcgtgatc ttggtgacgt tagtcgccgt cgggaaggca
    13201 caaaagccat tgagtaccac attgattgtc tgcctgaaat aacacgaaag gcactgcgtg
    13261 aacgctatgt ggaacagctg gtggctacag agaacaatgt ttctgaagtg aaagctgtta
    13321 ccagaaaaac acgcaatcct gacgctgtcc aggcaatcga agcatatcgc ggttctccac
    13381 aactgatgga agaacgcctg aatgcgctga ccgaaaacca gcgctgggta tctgaagcaa
    13441 gagctgcgct ggtggtggaa gtgctgaagc tggaaagcgc cggtaacccc gggcgactga
    13501 aagccattaa ctttcttgtt gaaaaagccc gtaaaggtga gctgccggag cgcctgcaac
    13561 aggccgcagt taacgccaat gcaaaacgtg gcgctaatcg tactatcagc cgcgatccac
    13621 tttaccagtg ggttctgaaa tacaaccagt cgcagaacgc cgccgaacgg ctgctgttac
    13681 tggcaccggg aaaacgcgac gaaataaaac cggaagaaat cagctggctg cctgaatttc
    13741 tggcgcagta tcgccaggtg aacggcagac ccatgtcaga agcctacgag gattttgtcg
    13801 cagaatggca gcgtcgccat gcggacgaac cgtacatgct ggaggtgatg ccttcctatg
    13861 atgttgtccg gtatgccatg aaaaaactgc cggaagtggt gaaacaaaaa gggcgcgtta
    13921 ccggcagtga ataccgtcag cttgaaggat tcacccgccg cgactggaca gctatgccgg
    13981 tgaattatgt ctggattggt gacggtcacg gtatgaagct gaaatgtgcg catccgatcc
    14041 acgggcgtcc attctcaccg gaagtcacgt ttgtgatcga tggtggaaca cggtttgttg
    14101 ttggctggag tcttgacctt gccgaaaacg tgttcgccgt tgccggtgcg atacagcacg
    14161 gcattcgcaa ccacggaaaa ccgttcctgt attactcgga taacggttcc ggtgaaacgg
    14221 ctgacatgct ggacaaagag gtcgtgggta ttctgccacg cctggggatt aaacacccta
    14281 ccggtattgc cggtaatcca cagggacgcg gcattattga acgcctgaac cggacactgc
    14341 cgatgcgtat agccaggagg tatcgcacct attttggcaa gggtgctgac cgggaatcgt
    14401 tgcgtgtact taaccgtgat ctgcgttcag cgtttaacgc cctgcaacag gacaagcctc
    14461 tgaacgaccg ccagaaagcg gcaatgcgcg agctgccatc gtgggcggag cttattgagg
    14521 ctattcgtga aggcgtggaa tggtacaaca accgcccgca ttctgagctg ccgatgaaac
    14581 cgaacggacg gcattacagc ccgacagagt tcagaaaaaa acgccaggca gaagaagaca
    14641 ccgaaattga gtggctgagt gatcttgagc tgcgcgacat gttccgccca atggtggaac
    14701 gaccggtaag acgctgcgaa attcagtggc tgaacaatat ttactacgcg ccggagttgc
    14761 gtgacgaaca cggtcgcaaa gtgcttatca gttatgacat tcatgacgcc gagcgaatta
    14821 cggtacgtcg caaggacggc agctttatct gtgaggcgat atggaacggc aataaacgcg
    14881 cagcctttgc tgtcagtgct gaatatcaca aacaacagca gcgtattaaa ggaatgcgca
    14941 aacgcgctga ggaaaaaatt cgtgatgctg aagatgaagg tattcagatt ctggaacaca
    15001 aacaggctga accctggctg agtaatgttt atcgcccggt tggtaatgtg gtggcagttc
    15061 agcaaccgga atatgaagaa gagcatgacg aagaatttga acgcgatttc cggctgggga
    15121 tgcaaaaact ttttgccatg caggaagaag atgatccgct ggcctgaata aaaaaacagt
    15181 ccgcgggaac ggactgttaa ataaaggaaa caaagctgat ttgaaataac cgaggaaata
    15241 ctatgactga tattgatgat gtttgaggtg tactggcaat agcggacact accatttgtt
    15301 ctttttttaa gcagccatct gatgatattt ttccctgaag gctgccgggg agatatttga
    15361 ggtgtactgg caatagcgga cactaccatt tgttcttttt ttaagcag
//
LOCUS       DAZXUK010000083        15131 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZXUK010000083 DAZXUK010000000
VERSION     DAZXUK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15131
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.083"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            55..936
                     /locus_tag="ACH3L8_002003"
     CDS             55..936
                     /locus_tag="ACH3L8_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFT4085131.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(971..2305)
                     /gene="srmB"
                     /locus_tag="ACH3L8_002004"
     CDS             complement(971..2305)
                     /gene="srmB"
                     /locus_tag="ACH3L8_002004"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFT4085132.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELHPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            2437..3174
                     /gene="trmN"
                     /locus_tag="ACH3L8_002005"
     CDS             2437..3174
                     /gene="trmN"
                     /locus_tag="ACH3L8_002005"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFT4085133.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(3159..4781)
                     /gene="nadB"
                     /locus_tag="ACH3L8_002006"
                     /pseudo
     CDS             complement(3159..4781)
                     /gene="nadB"
                     /locus_tag="ACH3L8_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            5037..5192
                     /gene="rseD"
                     /locus_tag="ACH3L8_002007"
     CDS             5037..5192
                     /gene="rseD"
                     /locus_tag="ACH3L8_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFT4085134.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            5189..5764
                     /gene="rpoE"
                     /locus_tag="ACH3L8_002008"
     CDS             5189..5764
                     /gene="rpoE"
                     /locus_tag="ACH3L8_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFT4085135.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            5797..6447
                     /gene="rseA"
                     /locus_tag="ACH3L8_002009"
     CDS             5797..6447
                     /gene="rseA"
                     /locus_tag="ACH3L8_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFT4085136.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            6447..7403
                     /gene="rseB"
                     /locus_tag="ACH3L8_002010"
     CDS             6447..7403
                     /gene="rseB"
                     /locus_tag="ACH3L8_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFT4085137.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            7400..7879
                     /gene="rseC"
                     /locus_tag="ACH3L8_002011"
     CDS             7400..7879
                     /gene="rseC"
                     /locus_tag="ACH3L8_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFT4085138.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            8077..9876
                     /gene="lepA"
                     /locus_tag="ACH3L8_002012"
     CDS             8077..9876
                     /gene="lepA"
                     /locus_tag="ACH3L8_002012"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFT4085139.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            9892..10866
                     /gene="lepB"
                     /locus_tag="ACH3L8_002013"
     CDS             9892..10866
                     /gene="lepB"
                     /locus_tag="ACH3L8_002013"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFT4085140.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            11138..11818
                     /gene="rnc"
                     /locus_tag="ACH3L8_002014"
     CDS             11138..11818
                     /gene="rnc"
                     /locus_tag="ACH3L8_002014"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFT4085141.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            11815..12720
                     /gene="era"
                     /locus_tag="ACH3L8_002015"
     CDS             11815..12720
                     /gene="era"
                     /locus_tag="ACH3L8_002015"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFT4085142.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            12732..13460
                     /gene="recO"
                     /locus_tag="ACH3L8_002016"
     CDS             12732..13460
                     /gene="recO"
                     /locus_tag="ACH3L8_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFT4085143.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            13472..14203
                     /gene="pdxJ"
                     /locus_tag="ACH3L8_002017"
     CDS             13472..14203
                     /gene="pdxJ"
                     /locus_tag="ACH3L8_002017"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFT4085144.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            14203..14583
                     /gene="acpS"
                     /locus_tag="ACH3L8_002018"
     CDS             14203..14583
                     /gene="acpS"
                     /locus_tag="ACH3L8_002018"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFT4085145.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            15003..15083
                     /gene="shoB"
                     /locus_tag="ACH3L8_002019"
     CDS             15003..15083
                     /gene="shoB"
                     /locus_tag="ACH3L8_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFT4085146.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT         3672 a         3725 c         4079 g         3655 t
ORIGIN      
        1 aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg ggttatggat
       61 ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt gcgagcttcg
      121 caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct tgagcaggag
      181 ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg cgaaggaaaa
      241 aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg tgaggctgcc
      301 aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac gttgctctct
      361 taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt gcgtttatcg
      421 ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga ggccgatgtc
      481 ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt gggtgaacaa
      541 tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga gccgggaaga
      601 cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca gatatttgag
      661 agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat aagtatcgaa
      721 agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca ctttgcggtg
      781 gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca gtcgcagacc
      841 attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat gcacattttt
      901 attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg gcttaaaccc
      961 ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc ggtttacggc
     1021 gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc tctttctctt
     1081 tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg ccggtctgct
     1141 tctcgcttgg cgcacgcgtt ttcgggtgta actcatcaat aacgcgagct ttaatcggct
     1201 cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc accagcgaaa
     1261 ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa taagtatcgc
     1321 cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg ataccgcgcg
     1381 cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt ttaattgctt
     1441 cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg cccgcttcgc
     1501 gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg attgagcgag
     1561 tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc aggtcatcgg
     1621 cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg gcagaaactt
     1681 ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct tccagcgttg
     1741 ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt tcgatatcct
     1801 gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt tcaaccgcgc
     1861 ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg gcgaccacga
     1921 tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg ccggtgatgg
     1981 tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc acctgcatcg
     2041 ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc ttacgtggga
     2101 aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg gtgcctgtcg
     2161 gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca gcctgaatgg
     2221 cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg tcgagttcaa
     2281 gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga ttatagacgt
     2341 tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg ttctcacccg
     2401 gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac atccgtgctt
     2461 cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg tgcgatgaaa
     2521 gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt gaaacgttgc
     2581 cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg aaccagtgac
     2641 agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc gcaggaaaat
     2701 atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat tcagcagtgg
     2761 atcacacagc agacagtacg cttcgattta atcatcagta acccacctta ctatcagcag
     2821 ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac gcttgatcac
     2881 ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt ctgcgtggtg
     2941 ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg ctggcattta
     3001 cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt gctgctggca
     3061 ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg gccagaccag
     3121 aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat gtaatgattg
     3181 ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc cagcgtaaaa
     3241 tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc ggcaacctgt
     3301 accagattac gcagctccag caaattattt gagacgcgga aatgggcgta atattcgtct
     3361 atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt cgttgtgcgc
     3421 acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg ctgaattact
     3481 acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt actgacgccg
     3541 tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata gaccagacac
     3601 tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata actcacctcg
     3661 ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat tacaccacct
     3721 caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc aatccccagc
     3781 ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc gggcttatgg
     3841 ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc aatggcgcgg
     3901 gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat aaaacgcgtg
     3961 ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa caggaaattg
     4021 cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag attggcaacc
     4081 cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc cggattggtg
     4141 gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt tgcgtggcag
     4201 gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg tcgcgtgccc
     4261 ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc cagcacacga
     4321 atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct accggtggcg
     4381 tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag atggtagctt
     4441 tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat taaccatggc
     4501 acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca aataccagcc
     4561 ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc aaacacggcg
     4621 gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt acttagaacg
     4681 atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc gccgctacca
     4741 ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt ttaatttact
     4801 aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct attttaatat
     4861 ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag ttactggctg
     4921 gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc agttaaatgg
     4981 gcatttctac acagataatg cgatgttcag attctgtaga cttataatga tagataatga
     5041 tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa aaacgagaca
     5101 ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat agcgcgtgga
     5161 aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc aggtcctggt
     5221 tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc gctatcagca
     5281 taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg atgtggtaca
     5341 agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata gcgcttttta
     5401 tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg ctcaggggcg
     5461 tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa gtggcggcgc
     5521 gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga gacagatagt
     5581 tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct tgcgggagct
     5641 ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag gtacggtgcg
     5701 ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc ttatcaggcg
     5761 ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca actttccgct
     5821 ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca taacccagaa
     5881 atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg tgatactccc
     5941 gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga agagccagta
     6001 cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg gcagaaaatg
     6061 ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg cgtagccgca
     6121 tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc tgaaacgtcc
     6181 cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc cagcccggta
     6241 agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca ggtacaggag
     6301 cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg actccactct
     6361 gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt gccaggaatt
     6421 caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg tcattagtga
     6481 caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg gcgttattac
     6541 agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc agcatcaata
     6601 aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt cctcttgcac
     6661 aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat gaaatcagct
     6721 attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt gattctctgc
     6781 catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt atctccgtcg
     6841 ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc cgagatggta
     6901 cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg cgggttgatc
     6961 tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt aacgtcaatc
     7021 aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg ttgctttctg
     7081 ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca cagggtttta
     7141 gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct atcgaatcac
     7201 gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct acgccatcga
     7261 gcaccgatca gatgttgcgc accggacgca gaactgtcag tacaagcgta cgtgataacg
     7321 ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt gccgagaata
     7381 ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct ggcaaaacgg
     7441 gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt cacgcgccgg
     7501 ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca ttgtcgtacc
     7561 ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg aaggcagcct
     7621 gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc tcatcgcttc
     7681 gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg tcctcggcgg
     7741 tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc gggcagaatg
     7801 gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg aaacatcttc
     7861 ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca aatgcgagga
     7921 cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat gttgtagtgt
     7981 agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt ctaaaagcct
     8041 ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg taacttttcg
     8101 atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat ccagatctgc
     8161 ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga tcttgagcgt
     8221 gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc gtctgacggc
     8281 gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc ctatgaagtt
     8341 tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg gcagggcgta
     8401 gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga agtggtgccg
     8461 gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga agaaattgaa
     8521 gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac cggcgttggc
     8581 gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg cgatccggaa
     8641 ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg cgttgtttca
     8701 cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt catgagtacc
     8761 gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt tgaccgcact
     8821 gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat ccacggcgct
     8881 ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct gcctggcttt
     8941 aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga cgactatgaa
     9001 gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt ctatgagccg
     9061 gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct gctgcacatg
     9121 gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac cactgcgccg
     9181 accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga cagcccatcc
     9241 aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga gtgtcacatg
     9301 ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa acgcggcgtg
     9361 cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat cccgatggcg
     9421 gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta tgcgtctctg
     9481 gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt attaatcaac
     9541 ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca aaaccgcggt
     9601 cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga tatcgccatt
     9661 caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct gcgtaaaaac
     9721 gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct gcagaagcag
     9781 aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca ggaagcgttc
     9841 ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg catggcgaat
     9901 atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg gtgcgtggat
     9961 aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc ggctgccggt
    10021 gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg gctggaaacc
    10081 ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt tatttatgaa
    10141 ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga ttttattctg
    10201 gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct gatcgaaacc
    10261 ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc aaagcttgat
    10321 tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc ggtctcaaaa
    10381 gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc gctgccggtc
    10441 acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg taatggtggg
    10501 gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa tggaattcgt
    10561 ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac agtgccgatt
    10621 gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc aacctggatt
    10681 gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc ggacagccgt
    10741 tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat ctggatgagc
    10801 ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat tggcggcatc
    10861 cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga attatttatg
    10921 agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata ttcccccgaa
    10981 gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt ccccatatac
    11041 cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg tctgtttcgt
    11101 gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg taattaatcg
    11161 gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc aggcattaac
    11221 tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg actctattct
    11281 gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg aaggcgatat
    11341 gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg cgcgcgaatt
    11401 tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg gatttcgtcg
    11461 tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc tcgacagtga
    11521 tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg acgaaattag
    11581 cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc agggtcgcca
    11641 tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg atcaggaatt
    11701 tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag gttcaagccg
    11761 tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc tggaatgagc
    11821 atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt tggcaaatcc
    11881 acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa ggcgcagaca
    11941 actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat ctacgtcgat
    12001 acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa caaagcggcg
    12061 agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac ccgctggacg
    12121 ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt aatcctcgcg
    12181 gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct gcagttcctg
    12241 gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg gctgaatgtt
    12301 gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca cttcccggaa
    12361 gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg cgaaaaactg
    12421 atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga acgtttcgtc
    12481 tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg tgaagggcag
    12541 aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat tgaagcgcgt
    12601 aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt aaaagtgaaa
    12661 tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga cgatctttaa
    12721 gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc ccgtggagcg
    12781 aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt ctggttgcca
    12841 aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc acccctctct
    12901 tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa gctgtctcac
    12961 tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac gaacttctct
    13021 cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac ttgcactgca
    13081 ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc tttgaactgg
    13141 cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt agcggcgagc
    13201 cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc gcaagcgtcg
    13261 ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg cgggaatttc
    13321 ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt aagccgtatc
    13381 ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct aagcgaacgg
    13441 tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag gcgtcaacat
    13501 tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc cggtgcaggc
    13561 ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac gtgaagatcg
    13621 ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata cccgaatgaa
    13681 tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga agccacattt
    13741 ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc tggacgttgc
    13801 agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg ggattcaggt
    13861 ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg ttggcgcacc
    13921 gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg aacaggcgca
    13981 agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc tgaaagttaa
    14041 cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc ctgagatgca
    14101 tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac tgaaagatgc
    14161 ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat attaggttta
    14221 ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc cggtgagcgc
    14281 ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca ccaccagccg
    14341 gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc gtttggcacc
    14401 gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga gctcggcaaa
    14461 ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg cgttgtaaat
    14521 atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat tattgaaagt
    14581 taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga acgggggttt
    14641 tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt atggatcagt
    14701 gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc ctttacgaag
    14761 aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat taaaattggc
    14821 ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc cattgaaacg
    14881 agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa ctcgcacgtt
    14941 cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt aaggaaaaac
    15001 gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt gctgttttac
    15061 agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc cggtgaacca
    15121 atcggacgcg c
//
LOCUS       DAZXUK010000084        14706 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZXUK010000084 DAZXUK010000000
VERSION     DAZXUK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14706
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.084"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            81..350
                     /locus_tag="ACH3L8_002020"
     CDS             81..350
                     /locus_tag="ACH3L8_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFT4085147.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="ACH3L8_002021"
     CDS             344..709
                     /locus_tag="ACH3L8_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFT4085148.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACH3L8_002022"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACH3L8_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFT4085149.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="ACH3L8_002023"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="ACH3L8_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFT4085150.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="ACH3L8_002024"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="ACH3L8_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFT4085151.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="ACH3L8_002025"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="ACH3L8_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFT4085152.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACH3L8_002026"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACH3L8_002026"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFT4085153.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACH3L8_002027"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACH3L8_002027"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFT4085154.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEVPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="ACH3L8_002028"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="ACH3L8_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACH3L8_002029"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACH3L8_002029"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFT4085155.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACH3L8_002030"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACH3L8_002030"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFT4085156.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT         3418 a         4091 c         3657 g         3540 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgcat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct catggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aagtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggca cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctagt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataat
//
LOCUS       DAZXUK010000085        14694 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZXUK010000085 DAZXUK010000000
VERSION     DAZXUK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14694
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.085"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..714)
                     /gene="trpA"
                     /locus_tag="ACH3L8_002031"
     CDS             complement(<1..714)
                     /gene="trpA"
                     /locus_tag="ACH3L8_002031"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFT4085157.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIV"
     gene            complement(714..1907)
                     /gene="trpB"
                     /locus_tag="ACH3L8_002032"
     CDS             complement(714..1907)
                     /gene="trpB"
                     /locus_tag="ACH3L8_002032"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFT4085158.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(1919..3280)
                     /gene="trpCF"
                     /locus_tag="ACH3L8_002033"
     CDS             complement(1919..3280)
                     /gene="trpCF"
                     /locus_tag="ACH3L8_002033"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFT4085159.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3281..4876)
                     /gene="trpD"
                     /locus_tag="ACH3L8_002034"
     CDS             complement(3281..4876)
                     /gene="trpD"
                     /locus_tag="ACH3L8_002034"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFT4085160.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(4876..6438)
                     /gene="trpE"
                     /locus_tag="ACH3L8_002035"
     CDS             complement(4876..6438)
                     /gene="trpE"
                     /locus_tag="ACH3L8_002035"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFT4085161.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6460..6553)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6530..6574)
                     /gene="trpL"
                     /locus_tag="ACH3L8_002036"
     CDS             complement(6530..6574)
                     /gene="trpL"
                     /locus_tag="ACH3L8_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFT4085162.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6712..7593
                     /gene="rnm"
                     /locus_tag="ACH3L8_002037"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6712..7593
                     /gene="rnm"
                     /locus_tag="ACH3L8_002037"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFT4085163.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7590..8210
                     /locus_tag="ACH3L8_002038"
     CDS             7590..8210
                     /locus_tag="ACH3L8_002038"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFT4085164.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8311..9183
                     /gene="rluB"
                     /locus_tag="ACH3L8_002039"
     CDS             8311..9183
                     /gene="rluB"
                     /locus_tag="ACH3L8_002039"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFT4085165.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(9223..9813)
                     /gene="cobO"
                     /locus_tag="ACH3L8_002040"
     CDS             complement(9223..9813)
                     /gene="cobO"
                     /locus_tag="ACH3L8_002040"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFT4085166.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(9810..10568)
                     /locus_tag="ACH3L8_002041"
     CDS             complement(9810..10568)
                     /locus_tag="ACH3L8_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFT4085167.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            10788..11837
                     /gene="sohB"
                     /locus_tag="ACH3L8_002042"
     CDS             10788..11837
                     /gene="sohB"
                     /locus_tag="ACH3L8_002042"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFT4085168.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(11873..12124)
                     /locus_tag="ACH3L8_002043"
     CDS             complement(11873..12124)
                     /locus_tag="ACH3L8_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFT4085169.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12504..>14694
                     /gene="topA"
                     /locus_tag="ACH3L8_002044"
     CDS             12504..>14694
                     /gene="topA"
                     /locus_tag="ACH3L8_002044"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFT4085170.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMA"
BASE COUNT         3539 a         3778 c         3952 g         3425 t
ORIGIN      
        1 aacaatggcc gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg cttttacctg
       61 atccggggcg gaaataccaa acccctgcaa tggaggtgca gcgttgtact ctttcagctt
      121 cgcaaccaga tgattgaggg gtaacgcggc gcggttttct gcgccggtca cgcccgctcg
      181 tgacagcaaa tatgtgtaac cacgaccgta agaggctatc tggcgcagca ggtcgtcatc
      241 ggcatttggc gggcagatga agataggtgc gacattatgg cgcaacgcgg cctggcggaa
      301 gggcgcggac tcttcaactg gcacatcggc aaccagcacc gaatcgacgc cgactttctc
      361 gcactgggca taaaactcat caatgccttt gttaaacacc agattggcat acatcaacag
      421 gccaatggga atggtcgggt gtttctggcg aatcagtgcc agcatttcaa aacattgtgc
      481 cggagtcaca cctgccgcaa aggcgcgcag agtggcgttt tgaatggtcg ggccatccgc
      541 cagtgggtcg gagaagggga tacctaactc cagcgcgtca gcaccggctt caattagcgt
      601 atcgataatt ttcaatgact gctcaatgcc cggatcaccg agcgtgacga aaggaacgaa
      661 tgcgccttct ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt ccatcagatt
      721 tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa
      781 aggttaacca ccagtagctg ctctttatcc gggttttcgc gcatcatttt cagcgcatgg
      841 gccagggcgt gggaggattc cagcgccggg atgatccctt cgtgcaggca cagcgttttg
      901 aaggcttcaa gggcttcatc atcggtaata gacacgtaat cagcgcgtcc agtgctgtta
      961 agatacgcgt gttgtggacc gacagacggg aaatccagtc cggcggagat ggagtaagat
     1021 tcttcaatct gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa ataaatgccc
     1081 acgcgaccat gttttagcgg tgcgccgtgc tcgccagttt cgataccgtg accaccaggc
     1141 tccacaccaa tcaggccgac gttggtttca ttgatgaaat cagcaaacat gccgatggca
     1201 ttcgaaccgc cgccaacaca ggcgataacg gcatccggca ggcgaccttc tctttccaga
     1261 atctgcgctt tggtttcttc gccaatcatc cgctgaaact cacggacaat ggtcggataa
     1321 ggatgcgggc cagccgcggt gcccagcata tagtgcgcgg tttcataact gccggaccag
     1381 tcgcgtagcg cctcattaca ggcatctttc agcgtggcgg aaccgctatg taccgggatc
     1441 acttccgcac ccattaagcg catacgaaaa acgttaggcg actggcgttc aacgtctttg
     1501 gcacccatat aaatacggca tttcaggccg agcagggcgc tggcaagggc cgacgccacg
     1561 ccatgctgac cggccccggt ttcggcgatg atttcggttt tacccatccg cttcgccagc
     1621 aacgcctgcc ccagcacctg gttggtttta tgtgcgccgc cgtgcagcaa atcttcacgc
     1681 ttgagataca gcgtggtgtt cgtcccggct gtaatgttct ggcatttggt cagtgcggtt
     1741 ggacgcccgg catagttttt cagcaggtcg ttgaactggg cttgaaattc aggatctttt
     1801 tgcgcgctga caaaagcttc ttccagctgg cgcagagcag gcatcaggat ttgtggcacg
     1861 tacatgccgc caaactcacc aaaatagggg ttaagtaatg ttgtcattgt tcctttcctt
     1921 aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg atgcccggtt
     1981 gcgactctac agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc gcttccacgc
     2041 agttatctgc gcctaagccc cccgccagca gaacgttgcc aagcgattga ccatttaata
     2101 gtgaccagtc aaaacgttgc ccgcttccac cctggccgtt gtctaaaaca tatttatcaa
     2161 cgtgctgaaa ctcgcgggcg ggcagggttt caccgacgct taatgctttc cagatggcaa
     2221 catgtgctgg cagagcttca cgcagcgtat cgatatacag ctgatcttca ttgccatgca
     2281 gttgcactgc cgccagcgat aacaccttag ctttgtccac cacatcggca atatcgtgat
     2341 tgcggaacac gccaacatac tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa
     2401 cgttgacgca acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt
     2461 cataagctgc tttagcatct tgcccacgcg tcaggccaca tactttattc tcacccagca
     2521 acacccggcg cacggcggcg tgcaaatcgt catgggccat caacgccgaa ccaatcagaa
     2581 aaccgttagc gaagtggctt aactcgcgca cctgagcgta agtattgatg ccggattcgc
     2641 tgattaccgt tacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg ttgagatcaa
     2701 tcgacaaatc acgcagatcg cggttgttga tgccaacgac ctttgcccct aatgcaatgg
     2761 cgcgctccag ttcctcttca ttactgactt cggtcagcac ccccatctcc agactgtgag
     2821 cgacggctgc aagctggcga tattggtcgt catccagtac tgaaagcatt aataagcagg
     2881 catcggcctg gtaatagcgc gccagataga tctggtaagg gtcgataatg aagtctttac
     2941 ataaaatcgg ctgcggggcg atttggctga cgatgaggag gaaatcaaag ctcccctgaa
     3001 aatatttctc atcagtcagc accgaaattg ccgaagcgta atgtttataa atggcggcaa
     3061 tgcgtgccgg atcgaaatca tcacggatca cgccttttga cggcgacgct tttttacact
     3121 ccagaataaa cgccgtgcgt gcaccctgta gcgcatcata aaaatgtcgt gtgctcggct
     3181 gaacctcatt ctgaaaactg gccagcggtt gctgctgttt gcgggtttct acccaaatcg
     3241 ccttgtctgc gacgattttc gctaaaacgg tttgcatcat ttaccctcgt gccgccagcg
     3301 cggtaactct gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt tgcgcattgg
     3361 cttgcagatc ttcatggcca tgcaggcgca ttaacatggc gacgttcgca gcgacggctg
     3421 cttcatgggc ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca cggttttctt
     3481 caggtgttcc gcctgccagt tgctcctggt ggtagggagt caggccaaaa tcttcagcgg
     3541 tcaattgata gctcttaatt ttgccgtcat gcagctcggc aacgattgtc ggcgcgtgta
     3601 atgaaacttc atccatcccg ccgctgtgca ccaccgccgc gcgttgatac cccagcacgc
     3661 gcaaggtttc ggcaatcggc agcaccagtt ccggactata aacaccaatt aacgccagcg
     3721 gcggatgcgc cgggttaatc aatggcccca gcacattgaa cagggtgcgg gttttcagtt
     3781 gctggcgaac cggcatcgcg tggcggaatc cggtgtgata tttcggcgca aagaggaaac
     3841 atacgcctaa ctcgtccagc gcctggcgcg atttattggc gttcatatca agattaatac
     3901 cgaatgccgc cagcagatcc gacgagccgg atttactgga gacgctgcgg ttaccgtgtt
     3961 tcgccacttt cagcccacag gccgcggcga caaacgcact ggcggtagaa atattgatac
     4021 tgttgctgcc gtcaccgcca gtaccgacga tatcagcaaa cagataatcc gggcgcggga
     4081 acggcgctgc gttttccagt agcgcggttg ctgccccggc gatctcgttc gggtgctcac
     4141 cgcgaatttt catgctcacc agcgccgccg ccagttgttc cggcttcagc tcgccacgca
     4201 ccaccgctga aaacagctgg tggctttctt gttggctaag cgtctgcgcc tgatacagtt
     4261 tttccagaat cggttgcagc gtgttggctg gctctagttt ctgctgcgcc caggccagcg
     4321 tttgttccag caggcgagcg ccctgggtgg tgagaatgga ttccggatgg aactggaatc
     4381 cacaaacgcg atccgcatcg tgacgtactg ccatcaccat gccattaaaa tgggcgttga
     4441 tggttaaacc ggccggaatg ttactgccaa ccagcgagtg ataacgcgcc accggcagcg
     4501 ggtttgttaa tccggcaaac atcgcctgac cgtcatgttc aatgctcgac gctttaccgt
     4561 gaagaatttc gcccgcctga ccgacatagc ccccgtaagc ttcgacaatt gcctgatgtc
     4621 cgaggcaaat gccaataatg ggcagcttgc cacgcaagcg ggtgaggagt tccggcatac
     4681 aaccggcttc gctcggcaca ccggggccag gagaaagcat cagtaccgga ttgctcatcg
     4741 tcgccaggcg ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc accacgttat
     4801 gaccattgct gcgcaactga tctgccaggt tgtacgtaaa agagtcgata ttatcgagca
     4861 gcagaatgtc agccatcaga aagtctcctg tgcatgatgc gcggtggcaa tagcgcgcag
     4921 tacagcgcgg gctttgttac gggtttcgtc ggcttccgac tgcggaacag aatcaaggac
     4981 tacaccagca cccgcttgca cggtggcgat accgttttcc accagcgccg agcggatcac
     5041 aatgcaggtg tcgagatcgc catgcgcggt gaaataacct accgcgccgc cgtagctgcc
     5101 gcggcgacga ccttccgcct cggcaattaa ctgcatagcg cgtactttcg gcgcaccgct
     5161 taacgtcccc atattcatac aggcgcgata agcgtgcagg gcgtcaagat cgtgacgcag
     5221 ttcgccgact acgcgagaga cgaggtgcat cacataggaa taacggtcaa ctttggtgag
     5281 atcggcgatg tagcggctgc caggggtgca aatgcgtgcc agatcattac gggcgagatc
     5341 aaccagcatc agatgttcag acagctcttt atggtcagta cgcatttcca gttcaatacg
     5401 gctgtcgaga tctctgtcca gtgaaccatc ggcgcgacga ccgcgtgggc gtgttccggc
     5461 aatcgggtag atctcaatct ggcggctggt ggcatcatac ttgagcgagc tttccggcga
     5521 cgcgccaaat agggtgaaat cattatcctg cataaaaaac atgtacgggc tgggattact
     5581 ctttttcagc acgtaatagg ccgccagcgg tgacgggcag ggcagagaga aacggcgaga
     5641 tggcaccacc tggaaaattt ctccagcgcg aatcgctttt tgcaacaaac gcactacgcc
     5701 accgaactct tcatcgctct gattacattc acaacgcata tgcggcacgg aaaccactgg
     5761 cagcggcggc gcggcttcgg tcagttgctg acgtagttcg ttcaggcgag cagtgagacg
     5821 ttgtttttct tcttcattcg gagcaaacag gctggcctga atacgggtgc tttttttctg
     5881 atggtcaatc accatcagcg tttcagcgag ataaaaacag aaatcagggc agttattttc
     5941 cgctgacagt tgcggtaaat cttcaaatcc cgccacaagg tcataagaga acaggccgcc
     6001 gaagaacatt gcttctcgtt cttccttcgg tacattcaac agattctgca ataaacggaa
     6061 agcgtcaaaa accgaaaggg agcataagcg ggcgtcttca tccagcagtg gactgacagg
     6121 ggggaagcgc agcacacggc agtttggtga ttgttcactt tccacacccg caggcagggc
     6181 gttatccagt agtgccagca gggcttcgcc attgccggaa agtgcctgaa ttgtgacggt
     6241 gtcacctaat gctgtaatgc gcagcgcact gtctatcagc agcaggcttt ttaaatcatc
     6301 tttgctgtcg atatctgcgg attccagcag cagcgttgcc ggacgattcc cacacaactg
     6361 gtgaaaaagc gcagtcgggt tgtcgcgata agcgccttcg caggttagca gttcgagagt
     6421 cggtttttgt gtttgcattg ttattctcta attttgttca aaaaaaagcc cgctcattag
     6481 gcgggctggg tatctgattg ctttacgcat ggtgaataca ctgcccgttt caggaagtgc
     6541 gccaccaacc tttcagtacg aaaattgctt tcattgtcga tacccttttt acgtgaactt
     6601 gcgtactagt taactggttc gatgattaat tgtcaacagc tcatttcaga atatttgcca
     6661 gacccgttat gatgtcggcg caaaaaacat tatccagaac gggagtgcgc cttgagcgac
     6721 acgaattatg cagtgattta cgacctgcac agccatacca cagcttccga tggctgcctg
     6781 acgccagaag cattggtgca ccgtgcagtc gagatgcgcg tcggcaccct ggcgatcacc
     6841 gaccatgaca ccacagctgc aatcgcgcca gcaagagagg aaatttcacg ttcgggactg
     6901 gcgctgaatc ttattcccgg cgtggaaatt tccacggtct gggaaaatca tgaaattcat
     6961 attgtcgggc tgaatattga tattactcat ccgctaatgt gtgagttcct cgcgcagcag
     7021 acagaacggc gcaatcagcg ggcgcagcaa attgccgaaa gacttgaaaa agcgcaaata
     7081 cctggggcgc tggaaggcgc acaacgactg gcgcagggcg gcgcagtgac gcgcggtcat
     7141 tttgcgcgtt tccttgtgga gtgcggcaaa gccagttcaa tggcagatgt ctttaaaaag
     7201 tatctggcgc gcgggaaaac cggatacgtt ccgccacagt ggtgtacaat agaacaagct
     7261 attgatgtca ttcatcattc tggcggtaag gcggtgctag ctcatcctgg gcggtacaat
     7321 cttagtgcta aatggctgaa aagattggtt gcgcattttg ccgaacacca cggcgacgcg
     7381 atggaagtcg cgcagtgcca gcaatcgccc aatgaacgca cccagctggc cacacttgcg
     7441 cgtcagcatc atttatgggc atcacaagga tctgattttc atcagccatg cccgtggatc
     7501 gaactgggtc gtaaactctg gctgcccgca ggcgttgaag gcgtctggca gctatgggaa
     7561 cagccgcaga acaccacaga gagggaatta tgagccagtt tttttatatt catcctgata
     7621 acccgcagca acgtctgatc aaccaggcgg tggagatcgt gcgtaaaggc ggggtgattg
     7681 tctatccaac tgattccggc tatgcgctcg gctgtaaaat tgaagataaa aacgccatgg
     7741 agcgtatttg tcgtattcgc cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg
     7801 atctttctga actctcgacc tattcattcg tggataacgt tgcgtttcgt ttgatgaaaa
     7861 acaacacgcc gggcaactat accttcatcc tgaaggggac gaaggaagtg ccacgccgtc
     7921 tgttgcagga gaaacgcaaa accatcggta tgcgtgtgcc ttctaacccg attgcgcagg
     7981 cgttgcttga agcactgggc gaaccgatgc tttccacttc gctaatgctg ccaggcagcg
     8041 aatttaccga atccgatccc gaagaaatta aagaccgtct ggaaaaacag gtagatttga
     8101 ttattcacgg cggctatctc gggcagaaac caacaacggt tattgatctt accgatgata
     8161 cgccggtcgt ggtgcgtgaa ggcgtaggtg atgtgaagcc tttcttataa gtgcgtaact
     8221 ctgtatacta cgcggcctta aaatggcgct atacggcgcc attgattctc ctgttcgacg
     8281 cctgtgaagg cgacaccaaa aggaagctct atgagcgaaa aattacagaa agtgctggcc
     8341 cgcgctggcc acggctcccg ccgtgaaatc gaatctatta ttgaagccgg tcgtgtgagt
     8401 gttgatggca aaattgctaa actcggcgat cgcgttgaag ttacccctgg gctgaaaatt
     8461 cgtatcgatg gtcatctgat ttcggtacgt gagtccgctg aacaaatttg tcgcgttctg
     8521 gcctattaca aaccggaagg tgagttgtgt acccgtaacg atccggaagg gcgtccgacg
     8581 gtgtttgacc gtctgccaaa gctgcgtggc gcacgctgga ttgccgtagg tcgtctggac
     8641 gttaacacct gtggtctgct gctgttcacc accgatggtg aactggcaaa ccgtttaatg
     8701 catccaagcc gtgaagttga acgtgaatac gccgtgcgtg tatttggtca ggttgacgac
     8761 gcgaaactgc gtgatttgag tcgtggcgtg caactggaag atggtccggc agcttttaaa
     8821 accatcaagt tcagcggcgg cgaagggatc aaccagtggt acaacgtgac tctgaccgaa
     8881 ggccgtaacc gtgaagttcg tcgtctgtgg gaagcggttg gtgtgcaggt gagccgcctg
     8941 atccgtgttc gttacggtga tatcccactg ccaaaaggtc tgccacgcgg tggctggacg
     9001 gagctggacc tcgcccagac taactatctg cgcgaactgg tggagcttcc gccagaaacc
     9061 agctctaaag tcgctgtaga aaaagaccgt cgtcgcatga aggctaatca gattcgtcgt
     9121 gcggtgaaac gtcacagtca ggtgagcggc agtcgtcgtt ctggcgggcg taataacggt
     9181 taatcaaagt atgccggacg tcatatccgg catttttaca gattaataat caatccctat
     9241 ttgcgcctta acaccggcat caaacgcatg tctgatgggg cgcagctcac ttaccgtatc
     9301 cgccagttca agaatatccc gatgacagcc tcgacccgtg ataatcaccg tctgttgatg
     9361 tggacgttca tttaacgcct gcaccacttc ttccagtggc aaatagtcat acgccaccat
     9421 atacgtcagt tcatcaagca aaaccatatc cagtgaggaa tcagcaagca tccgctttgc
     9481 atgttgccag acttcgcggc aggcggcggt atcagactcg cggttttgtg tatcccaggt
     9541 aaagcccgtt gccatcacct gaaactcaac gccatgtggc tccagcagat tgcgttcgcc
     9601 attaggccag gtgcctttaa taaactgcac gacgcccact ttttttccgt gaccaactgc
     9661 gcgtgttgcc gtaccaaatg ccgcggtggt tttgcctttt ccattgccgg taaagacgat
     9721 gataataccg cgttcatcct gggcctgggc cacacgagca tctacttttt ctttcactcg
     9781 ctgctgacgc tgttggtagc gttcatcact cattgggaaa ttcctggttt acggcccggt
     9841 tgggcgtcaa aggtcatgcc ggttttacgg cggctgtcat cgcccatcag ccagaggtag
     9901 agcggcatga tatcagcggg tgttttaagt ttctgtggat cttcggtcgg gaaggcgctt
     9961 gcgcgcattg cggtgcgcgt accgcctggg ttaatgcagt tgacgcgcag gcgctgctga
    10021 tattcatcgg ccagtacctg catcatccct tcggtggcaa atttcgacgc tgcatatgca
    10081 ccccagttgg ctcgtccctg acgtccaacg cttgatgaag taaagaccag tgaaccggcg
    10141 tccgatttga gtaataaagg aagcagtgcc tgggtgagca taaaggtggc attaacgttg
    10201 atctgcatga cgtcctgcca gacctgcgga ttttgttcgc tcattgggca aacatcgccg
    10261 agcaatccgg cattatgcaa aacaccatcc agacgcggat aattaacgac aatgcgctgt
    10321 gccagttgtt ggcaattttc ggacgtgcag gtcagcaaat cgagaataaa ccactgtggc
    10381 tgacgcccag tttcttcgtt tatgtggctg gctacctgac gtaatttttc ttcattacgg
    10441 cccaacagaa tcactgtcgc accatagcgt gcatacgtca tcgcggcttc acgaccaatc
    10501 ccatcgctgg ctcccgtcac caggataatg cgatcattga gtaaatcttg ttttggctgg
    10561 taatgcatgg ctactcctca acgacgttgt ctgtcgcgct gccagtgtac tttatgactt
    10621 tggggcttta tgcctgaaac agaagggcat ttcaatcagc ctggggcgat aacaccgcaa
    10681 aatggatact ttgtcatact ttcgctgcaa taacatctct gcgagacggc ttaacatgcc
    10741 tgttgtaaac tgtgagccaa agcgttgttt aaccaaggtg gggactcgtg gaattgttgt
    10801 ctgaatatgg tttgtttttg gctaaaatcg ttaccgttgt gctagcgatt gcggcgattg
    10861 ccgccattat tgtcaatgtt gctcaacgta ataaacgcca gcgtggcgag ttacgggtca
    10921 acaatctcag cgaacagtat aaggagatga aagaagaact ggccgcggcg ctgatggact
    10981 cacatcagca aaaacagtgg cacaaagcgc agaagaaaaa gcacaagcaa gaagcgaaag
    11041 cagcaaaagc gaaagccaaa ctgggcgagg tggcaactga cagtaaacct cgcgtctggg
    11101 tgctggattt taaaggcagc atggacgccc atgaagtgaa ctcgctacgt gaagagataa
    11161 cggcggtact cgcagcattc aaaccgcagg atcaggttgt gctccgtctg gaaagccctg
    11221 gtggcatggt gcatggttac gggttggcgg cttcgcagct gcagcgtctg cgtgataaaa
    11281 acattccttt aactgttacg gtagacaaag tcgctgccag cggcggttac atgatggcct
    11341 gtgtggcgga caaaattgtt tccgcaccgt ttgctattgt gggttccatt ggggtggtgg
    11401 cgcaaatgcc caactttaac cgcttcctga aaagcaaaga tattgatatc gaactgcaca
    11461 ccgccgggca gtataagcgc acgctgacct tgctgggtga aaataccgaa gaagggcggg
    11521 agaaattccg cgaagagctg aacgaaacgc atcagttatt taaagatttt gtgaagcgta
    11581 tgcgtccgtc tctggatatt gaacaggtgg caacgggtga acactggtac ggacaacagg
    11641 cggtagagaa aggcctggtt gatgaaatca acaccagtga tgaagttatt cttagcctga
    11701 tggaaggccg tgaagtggtt aatgtacgct atatgcagcg taaacgactc attgaccgat
    11761 tcaccggcag cgcggcagag agcgccgatc gattgttgct acgctggtgg cagcggggtc
    11821 aaaagccatt gatgtaaaag acaaacgcga ggctaagacc tcgcgttttg ctttaatcaa
    11881 ccagatgata tttttctgaa agcacatggg ccaggtgttt gaacatatta aacaccgcgg
    11941 tgcttttggc tgttggcaat ccttgttcat ctaaaaagta gtcgccggta aataccagca
    12001 cgccattacg ctgcgtgacg ccggtggctt caatccctgc gagcgtatcc tcatgctcac
    12061 gaatgatttt gttggcctct ttcaacagcg tttcgcggtc gatgggttgt gtctctttgt
    12121 tcattattta ctccttaaac aaggacatta gtctacgcca ggcatggctt gcagacaaat
    12181 ataccacgct ggtggcaaga gcgccttact ggcaactttg gattttgcat gctaataaag
    12241 ttgcgtatcg gattttatca ggtacagtgt gacgctttcg tcaatctggc aatagatttg
    12301 cttgacattc gaccaaaatt ccgtcgtgct atagcgcctg taggccaaga cctgttaact
    12361 cagtcacctg aattttcgtg aacagagtca cgacaagggg ttgatatccg cagagagcga
    12421 gtccatatcg gtaactcgtt gccagtggaa ggtttatcaa cgtgcgacgc attcctggaa
    12481 gaatcaaatt aggtaaggtg aatatgggta aagctcttgt catcgttgag tccccggcaa
    12541 aagccaaaac gatcaacaag tatctgggtt gtgactacgt ggtgaaatcc agcgtcggtc
    12601 acatccgcga tttgccgacc agtggctcag ctgccaaaaa gagtgccgac tctacctcca
    12661 ccaagacggc taaaaagcct aaaaaggatg aacgtggcgc tctcgtcaac cgtatggggg
    12721 ttgacccgtg gcacaattgg gaggcgcact atgaagtgtt gcctggtaaa gagaaggtcg
    12781 tctctgaact gaaacaactg gctgaaaaag ccgaccacat ctatctcgca accgaccttg
    12841 accgcgaagg ggaagccatt gcatggcacc tgcgggaagt gattggtggt gatgatgcgc
    12901 gctatagccg agtggtgttt aacgaaatta ctaaaaacgc gatccgccag gcatttaaca
    12961 aaccgggtga gctgaatatt gatcgtgtta atgcccagca ggcgcgtcgc tttatggacc
    13021 gcgtggtggg gtatatggtt tcgccgctgc tgtggaaaaa gatcgctcgt ggtctgtctg
    13081 ccggtcgtgt gcagtcggtg gcggtccgcc tggtggtaga gcgtgagcgt gaaattaaag
    13141 cgttcgtacc ggaagagttc tgggaagtcg atgccagcac gactacgcca tctggtgaag
    13201 cgttggcgtt gcaggtgact catcagaatg acaaaccgtt ccgtccggtc aacaaagaac
    13261 aaactcaggc tgcggtaagt ctgctggaaa aagcgcgcta cagcgtgctg gaacgtgaag
    13321 acaaaccgac aaccagtaaa cctggcgctc cttttattac ctctacgctg caacaagctg
    13381 ccagcacccg tcttggtttt ggcgtgaaaa aaaccatgat gatggcgcag cgtttgtatg
    13441 aagcaggcta tatcacttac atgcgtaccg actccactaa cctgagtcag gacgcggtaa
    13501 atatggttcg cggttatatc agcgataatt ttggtaagaa atatctgccg gaaagtccga
    13561 atcagtacgc cagcaaagaa aactcacagg aagcgcacga agcgattcgt ccttctgacg
    13621 tcaatgtgat ggcggaatcg ctgaaggata tggaagcaga tgcgcagaaa ctgtaccagt
    13681 taatctggcg tcagttcgtt gcctgccaga tgaccccagc gaaatatgac tccacgacgc
    13741 tgaccgttgg tgcgggcgat ttccgcctga aagcacgcgg tcgtattttg cgctttgatg
    13801 gctggacaaa agtgatgcct gcactgcgta aaggcgatga agatcgtatc ttaccggcag
    13861 tcgataaagg cgatgctctg acgctcgttg aactaacacc agcccagcac tttaccaagc
    13921 cgccagcccg tttcagtgaa gcatcgctgg ttaaagaact ggaaaaacgt ggtatcggtc
    13981 gtccgtctac ctatgcgtcg atcatttcga ccattcagga tcgtggctat gtgcgagtag
    14041 aaaatcgtcg tttctatgcg gaaaaaatgg gcgaaatcgt caccgatcgc cttgaagaaa
    14101 atttccgcga gttaatgaac tacgacttta ccgcgcagat ggaaaacagc ctcgaccagg
    14161 tggcaaatca cgaagcagag tggaaagctg tactggatca cttcttctcg gatttcaccc
    14221 agcagttaga taaagctgaa aaagatccgg aagagggtgg tatgcgcccg aaccagatgg
    14281 ttctgaccag cattgactgc ccgacttgtg gtcgcaaaat ggggattcgc acagcgagta
    14341 ccggggtatt ccttggctgt tctggctatg cgctgccgcc gaaagagcgt tgcaaaacca
    14401 ccattaacct ggtgccggaa aacgaagtgc tgaacgtgct ggaaggcgaa gatgctgaaa
    14461 ccaacgcgct gcgcgcaaaa cgtcgttgcc cgaaatgcgg cacggcgatg gacagctatc
    14521 tcatcgatcc gaaacgtaag ttgcatgtct gtggtaataa cccaacctgc gacggttacg
    14581 agatcgaaga gggcgaattc cgcattaaag gttatgacgg cccgatcgtt gagtgtgaaa
    14641 aatgtggctc tgaaatgcac ctgaaaatgg ggcgtttcgg taaatacatg gcct
//
LOCUS       DAZXUK010000086        14669 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZXUK010000086 DAZXUK010000000
VERSION     DAZXUK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14669
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.086"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(9..1463)
                     /gene="xylB"
                     /locus_tag="ACH3L8_002045"
     CDS             complement(9..1463)
                     /gene="xylB"
                     /locus_tag="ACH3L8_002045"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFT4085171.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1535..2857)
                     /gene="xylA"
                     /locus_tag="ACH3L8_002046"
                     /pseudo
     CDS             complement(1535..2857)
                     /gene="xylA"
                     /locus_tag="ACH3L8_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            3223..4215
                     /gene="xylF"
                     /locus_tag="ACH3L8_002047"
     CDS             3223..4215
                     /gene="xylF"
                     /locus_tag="ACH3L8_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFT4085172.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            4293..5834
                     /gene="xylG"
                     /locus_tag="ACH3L8_002048"
     CDS             4293..5834
                     /gene="xylG"
                     /locus_tag="ACH3L8_002048"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFT4085173.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5812..6993
                     /gene="xylH"
                     /locus_tag="ACH3L8_002049"
     CDS             5812..6993
                     /gene="xylH"
                     /locus_tag="ACH3L8_002049"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFT4085174.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7071..8249
                     /gene="xylR"
                     /locus_tag="ACH3L8_002050"
                     /pseudo
     CDS             7071..8249
                     /gene="xylR"
                     /locus_tag="ACH3L8_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
     gene            complement(8357..9181)
                     /locus_tag="ACH3L8_002051"
     CDS             complement(8357..9181)
                     /locus_tag="ACH3L8_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFT4085175.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9501..11530
                     /gene="malS"
                     /locus_tag="ACH3L8_002052"
                     /pseudo
     CDS             9501..11530
                     /gene="malS"
                     /locus_tag="ACH3L8_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            11708..12961
                     /gene="avtA"
                     /locus_tag="ACH3L8_002053"
     CDS             11708..12961
                     /gene="avtA"
                     /locus_tag="ACH3L8_002053"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFT4085176.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13112..13585)
                     /locus_tag="ACH3L8_002054"
     CDS             complement(13112..13585)
                     /locus_tag="ACH3L8_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFT4085177.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(13687..14535)
                     /gene="yiaJ"
                     /locus_tag="ACH3L8_002055"
     CDS             complement(13687..14535)
                     /gene="yiaJ"
                     /locus_tag="ACH3L8_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFT4085178.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
BASE COUNT         3734 a         3722 c         3586 g         3627 t
ORIGIN      
        1 gataacgttt acgccattaa tggcagaagt tgctgataga ggcgacggaa cgtttcacgt
       61 cgtggctgat aagcggcata acgctgcgca tctggtagat gcgactgttc taacggtagc
      121 tgcggcaaca actcaatgag cgatttctct ggattcgccg caatctgcgc cagccttgct
      181 gcgcccagtg ctggccccac atcccccccc gtacggtaat cgagctgctg accgctgata
      241 tccgccagca tctgacgcca gtactcacta cgcgccccgc caccaatcaa cgtaacactt
      301 tgcggtttaa tgccgcaggc atgcacgaca tccatgccat ctgccagcgc ataacccacg
      361 ccttccagca ctgctcgcgc cagttcattg gggccatgtt gatgagtcaa accaaagaaa
      421 acccccttcg cctggggatt attgtgtggt gtacgctcgc cggaaagata aggcagaaac
      481 caaactggct cggcactttc atcagcctgt tgagctgcag cgattaaagc tgggacattg
      541 cacaggccgg ttaatttcgc ggcccaatcc agacacgatg ctgcactcag cattacagac
      601 attaaatgcc aacgctgcgg tagcgcatgg caaaagctgt gtacagcgct ttctggcttg
      661 cttaagaacc cttcgctgac agcaaaatag acccccgacg tccccagcga taacattgcc
      721 tgattagcat caaccattcc cacaccaact gcaccagctg cattgtcgcc accgcctgcg
      781 acaactggca ccgtcgccat accccacgct ttcgcaactt caggtaacaa agcaccagta
      841 atttcgctgc cttcgtataa tgcgggcatc tggtcacgag ataagtcgca agcctgcagc
      901 atgacgtcac tccagtcacg ctttgcgaca tccagccaca tggtgccagc tgcgtcagac
      961 atatcgctgg caaactcccc cgtcatacgc agacgcaagt aatctttcgg taataatact
     1021 ttgtcgattt gacggaatat ctccggctca tgccgctgaa cccatagcaa tttaggcgca
     1081 gtaaatccgg gcatcatcag gttgccggta atcactcgtg attgcggaac tctcgcttcc
     1141 agcaaagtgc actcttgcgc acagcgcccg tcgttccaca aaatggcagg gcgcaatacc
     1201 cgttgttgag catccagtaa ggttgctcca tgcatctggc cggcaatacc caatgcttta
     1261 acgtcctgca gagaatgctg atcgcccaga gctttcattg cgcgatcagt tgcctgccac
     1321 cactgttccg ggtcttgttc cgaccaaagt ggatgcgggc gcgaaacggt cagcttttcc
     1381 gtttgcgaag caaccacctc accctgctcg ttgagcaaaa taacttttac gcccgaggtg
     1441 ccaagatcta tcccgatata catatcgatc gttccttaaa aaaatgcccg gtatcgctac
     1501 cgataaccgg gccaacggac tgcacagtta gccgttattt gtcgaacaga taatgattta
     1561 ccagattttc cagttgctcc tggcgaccac tctgatgcac cggagacaaa ttatgttcct
     1621 gagcatattt ggctaaatct gccagtgaca tttggccttt caggatttgc tggcccaatt
     1681 cgctattcca gccggaataa cgctgcgcga tgcgtttatc cagctcgcca tcttcaatca
     1741 tgcgcgctgc aattttcagc gccagtgcca tcgtatccat cgcgccgata tgaccgtaaa
     1801 acagatcata tttatcagta ctttgacgac gtactttggc atcgaagttc agaccaccgg
     1861 tggtgaaacc gcctgctttg agaatttcat acatcaccag cgcattctct tccacactgt
     1921 tcgggaactg gtcggtgtcc cagcccagtt gcgcatcgcc acggttggcg tcgacagaac
     1981 cgaacaggcc aagcgcaatg gcggtggcta tttcatgatg gaaagagtga cctgccagcg
     2041 tcgcgtggtt agcttcaatg ttcagtttaa tctctttttc cagaccaaac tgtttcagga
     2101 agccatagac cgtcgcggca tcgtaatcat attgatgttt agtcggttct tgcggtttcg
     2161 gttcgataag caacgtgccc tagaagccga ttttatgttt atgctcaacc accatctgca
     2221 taaagcggcc cagttgttca cgctcctgac gcaagtcggt atttaacagc gtttcgtaac
     2281 cttcacgacc accccacagg acatagtttt caccgcccaa tttatgggtt gcttccatcg
     2341 ctgtaacaac ttgcgttgcc gcccagctga agacttcagg atctgggttc gtcgccgcac
     2401 ccgcgccata gcgagggttt gtaaagcagt tagcggttcc ccacagcagc ttcacgccgc
     2461 tctcttcttg cttggctgcc aggacatcaa ccatttgcgc aaaattattg atgtactctt
     2521 ttaacgacgc gccctcaggg gaaacatcca catcgtggaa gcaataaaat ggcacatgta
     2581 acttgtggaa aaactcaaat gcgacatctg ctttacgctt cgccaacgcc agtgcctcac
     2641 caggctgctg ccacggacga ttaaacgccc ccacaccaaa catgtccgcc ccgttccagc
     2701 agaaggtgtg ccagtagcag gcggcaaaac gcaagtgctc ttccatacgc ttacccaaca
     2761 ccagttcgtc gggattgtag tgacggaatg ctaacgggtt tgaggatttt gagccttcat
     2821 aacgaacgcg atcgagctgg tcaaaatagg cttgcatatt gaactccata atcaggtaat
     2881 gccgcgggtg atggatgatg tcgtaatatt gggcactccc tttcaattgc tcaattatgt
     2941 tatttcacac tgctattgag ataattcaca agtgtgcgct cgctcgcaaa ataaaatggg
     3001 atgatgaaaa tgggtaattc ctcgaagata aaaatgcaat aagtacaatt gcgcaacaaa
     3061 agtaagatct cggtcataaa tcaagaaata aaccaaaaat cgtaatcgaa agataaaaat
     3121 ctgtaattgt tttcccctgt ttagttgcta aaaattggtt acgtttatcg cggtgattgt
     3181 tacttattaa agctgtcctc taactacaga aggccctaca ccatgaaaat aaagaacatt
     3241 ctactcaccc tttgcacctc acttctgctt accaacgttg cggcacacgc caaagaagtc
     3301 aaaataggta tggcgattga tgatctccgt cttgaacgct ggcaaaaaga tcgagatatt
     3361 tttgtgaaaa aggcagaatc tctcggtgcg aaagtatttg tacagtctgc aaatggcaat
     3421 gaagaaacac aaatgtcgca gattgaaaac atgattaacc ggggtgtcga tgttcttgtc
     3481 attattccgt ataacggtca ggtattaagt aacgttgtaa aaaaagccaa acaagaaggt
     3541 attaaagtat tagcttacga ccgtatgatt aacgatgcgg atatcgattt ttatatttct
     3601 ttcgataacg aaaaagtcgg tgaactgcag gcaaaagcct tggtcgatat tgttccgcaa
     3661 ggtaattact tcctgatggg cggctcgccg gtagataaca acgccaagct gttccgcgcc
     3721 ggacaaatga aagtgttaaa accttacgtt gattctggaa aaattaaagt cgttggtgac
     3781 caatgggttg atggctggtt accggaaaac gcattgaaaa ttatggaaaa cgcgctaacc
     3841 gccaataata acaaaattga tgctgtagtt gcctcaaacg atgccaccgc aggtggggca
     3901 attcaggcat taagcgcgca aggtttatca gggaaagtag caatttccgg ccaggatgcg
     3961 gatctcgcag gtattaaacg tattgctgcc ggtacgcaaa ctatgacggt gtataaacct
     4021 attacattgc tggcaaatac tgccgcagaa attgccgttg aattgggcaa tggtcaggag
     4081 ccaaaagcag atacctcact gaataatggc ctgaaagatg tcccctcccg cctcctgaca
     4141 ccgatcgatg tgaataaaaa caacatcaaa gatacggtaa ttaaagacgg attccacaaa
     4201 gagagcgagc tgtaagcgtt acgccccggc gcggagcggg ggcgtgattt ctctccatgc
     4261 cgcgtgaatg aattggctta ggtggagtcg ttatgcctta tctacttgaa atgaagaaca
     4321 ttaccaaaac cttcggcagt gtgaaggcga ttgataacgt cagcttgcgg ttgaatgctg
     4381 gcgaaatcgt ctcactctgt ggggaaaatg ggtctggtaa atcaacgctg atgaaagtgc
     4441 tgtgtggtat ttatccccat ggctcctacg aaggcgaaat tatttttgcg ggagaagaga
     4501 ttcaggcgag tcacatccgc gataccgaac gcaaaggtat cgccatcatt caccaggaat
     4561 tggccctggt gaaagaattg accgtgctgg aaaatatctt cctgggtaac gaaataaccc
     4621 acaatggcat tatggattat gacctgatga cgctacgcta tcagaagctg ctcgcacagg
     4681 tcagtttatc catttcacct gatacccgcg ttggcgattt agggcttggg caacaacaac
     4741 tggttgaaat tgccaaggca cttaataaac aggtgcgctt gttaattctc gatgaaccga
     4801 cagcctcatt aactgagcag gaaacgtcgg ttttactgga tattattcgc gatctacaac
     4861 agcacggtat cgcctgtatt tatatttcgc acaaactcaa cgaagtcaaa gcgatttccg
     4921 atacgatttg cgttattcgc gacggacagc acattggtac gcgtgatgct gccggaatga
     4981 gtgaagacga tattatcacc atgatggtcg agcgagagtt aaccgcgatt taccctaatg
     5041 aaccacatac caccggagat gaaatattac gtattgaaca tctgacggca tggcatccgg
     5101 tcaatcgtca tattaaacga gttaatgatg tctcgttttc cctgaaacgt ggcgaaatac
     5161 tgggtattgc cggactcgtt ggtgccggac gtaccgagac cattcagtgc ctgtttggcg
     5221 tgtggcccgg acaatgggaa ggaaaaattt atattgatgg caaacaggta gatattcgta
     5281 actgtcagca agccatcacc caggggattg cgatggtacc cgaagacaga aagcgcgacg
     5341 gcatcgttcc ggtaatggcg gttggtaaaa atattaccct cgccgcactc aataaattta
     5401 ccggtggcat tagccagctt gatgacgcgg cagagcaaaa atgtattctg gaatcaattc
     5461 agcaactcaa agttaaaacg tcgtcccccg accttgctat tggacgtttg agcggcggca
     5521 atcagcaaaa agcgatcctc gcccgctgtc tgttacttaa cccgcgcatc ctcattcttg
     5581 atgaacctac caggggtatt gatattggcg cgaaatatga gatctacaaa ttaattaacc
     5641 aactcgtcca gcagggtatt gccgttattg tcatctcttc cgaattacct gaagtgctcg
     5701 gccttagcga tcgtgtactg gtgatgcatg aagggaaact aaaagccaac ctgataaatc
     5761 ataacctgac tcaggagcag gtgatggaag ccgcattgag gagcgaacat catgtcgaaa
     5821 agcaatccgt ctgaagtgaa attggccgta ccgacatccg gtggcttttc tgggctgaaa
     5881 tcactgaatt tgcaggtctt cgtgatgatt gcagctatca tcgcaatcat gctgttcttt
     5941 acctggacca ccgatggtgc ctacttaagc gcccgtaacg tctccaacct gttacgccag
     6001 accgcgatta ccggcatcct cgcggtagga atggtgttcg tcataatttc tgctgaaatc
     6061 gacctttccg tcggctcaat gatggggcta ttaggtggcg tcgcggcgat ttgtgacgtc
     6121 tggttaggct ggcctttgcc acttaccatc attgtgacgt tggttctggg actgcttctc
     6181 ggtgcctgga acggatggtg ggtcgcgtac cgcaaagtcc cttcatttat tgtcaccctc
     6241 gcgggcatgt tggcatttcg cggcatactc attggcatca ccaacggcac gactgtatcc
     6301 cccaccagcg ccgcgatgtc acaaattggg caaagctatc tccccgccag taccggcttc
     6361 atcattggcg cgcttggctt aatggctttt gttggttggc aatggcgcgg aagaatgcgc
     6421 cgtcaggctt tgggtttaca gtctccggcc tctaccgcag tagtcggtcg ccaagcttta
     6481 accgctatca tcgtattagg cgcaatctgg ctgttgaatg attaccgtgg cgttcccact
     6541 cctgttctgc tgctgacgtt gctgttactc ggcggaatgt ttatggcaac gcggacggca
     6601 tttggacgac gcatttatgc catcggcggc aatctggaag cagcacgtct ctccgggatt
     6661 aacgttgaac gcaccaaact tgccgtgttc gcgattaacg gattaatggt agccatcgcc
     6721 ggattaatcc ttagttctcg acttggcgct ggttcacctt ctgcgggaaa tatcgccgaa
     6781 ctggacgcaa ttgcagcatg cgtgattggc ggcaccagcc tggctggcgg tgtgggaagc
     6841 gttgccggag cagtaatggg ggcatttatc atggcttcac tggataacgg catgagtatg
     6901 atggatgtac cgaccttctg gcagtatatc gttaaaggtg cgattctgtt gctggcagta
     6961 tggatggact ccgcaaccaa acgccgttct tgattttgat aaaaattttc tcaaagccgg
     7021 ttacgtatta ccggttttga gtttttgcat gattcagcag gaaaagaacc atgtttacta
     7081 aacgtcaccg catcacatta ctgttcaatg ccaataaagc ctatgaccgg caggtagtag
     7141 aaggcgtagg ggaatattta caggcatcac aatcagaatg ggatattttc attgaagaag
     7201 atttccgcgc ccgcattgat aaaatcaagg actggttagg agatgtcgtc attgccgact
     7261 tcgacgacaa acagatcgag caagcgctgg ctgatgtcga cgtccccatt gttggggttg
     7321 gcggctcgta tcaccttgca gaaagttacc cacccgttca ttacattgcc accgataact
     7381 atgcgctggt tgaaagcgca tttttgcatt taaaagagaa aggcgttaac cgctttgctt
     7441 tttatggtct tccggaatca agcggcaaac gttgggccac tgaacgcgaa tatgcatttc
     7501 gtcagcttgt cgccgaagaa aagtatcgcg gagtggttta tcaggggtta gaaaccgcgc
     7561 cagagaactg gcaacacgcg caaaatcggc tggcagactg actacaaacg ctgccaccgc
     7621 aaaccgggat tattgccgtt actgacgccc gagcgcggca tattctgcaa gtatgtgaac
     7681 atctacatat tcccgtaccg gaaaaattat gcgtgattgg catcgataac gaagaactga
     7741 cccgctatct gtcgcgtgtc gccctttctt cggtcgctca gggcgcgcgg caaatgggct
     7801 atcaggcggc aaaactgttg catcgattat tagataaaga agaaatgccg ctacagcgaa
     7861 ttttggtccc accagttcgc gtcattgaac ggcgctcaac agattatcgc tcgctgaccg
     7921 atcccgccgt tattcaggcc atgcattaca ttcgtaatca cgcctgtaaa gggattaaag
     7981 tggatcaggt actggatgcg gtcgggatct cgcgctccaa tcttgagaag cgttttaaag
     8041 aagaggtggg tgaaaccatc catgcaatga ttcatgccga gaagctggag aaagcgcgca
     8101 gtctgctgat ttcaaccacc ttgtcgatca atgagatatc gcaaatgtgc ggttatccat
     8161 cgctgcaata tttctactct gtttttaaaa aagcatatga cacaacgcca aaagagtatc
     8221 gcgatgtaaa tagcgaggtc atgttgtagc gatgatgaga attgtcggcg tcacatcagg
     8281 gaactcagca cagccacctg atgcgagcaa aagccttatc cgaccgacaa atcagataag
     8341 gctcgaggaa atgaaattac atatgcgccg cgattaaccg ttggttatcc tggtacattg
     8401 cgaacaggta gttgttataa ctcttccctt tggtcgagta gcccttcagc ttgtgaatca
     8461 tcgctgtggc agtcacttcc tgatccgctt tacgcagctg tgcacgcgat ttacggaacg
     8521 aagagtaagc cgggtgcgta ttcaggttag tgacataggc gctcaccgat tctttgacag
     8581 aactaaactg tgagtaccct ttcactttac ccggcgcatt ggtacaacgt cctttcatgc
     8641 atttcatgcc gaacagattg ttattgttgc gcgccagctt cgacgttccc caaccgcttt
     8701 ctgctgcagc catcgtcgcc accatactgg tagggataat gtctacgcgt tcaagcaagg
     8761 tattccacgg gatttttcgc gtattaccag accacttcac cttgtagcgt ttggcgatgt
     8821 ctttcagacg cgcacgctca gcaggtgccc attggccctg atactgtttt gaaatcagcc
     8881 agttacgttc tgcagtaatg gctgcatttt ggctggtaat gtaaggcatt acggtccgga
     8941 gaaacgcctt tttccttggt gttccggaag ggtattttcg caaatcagga agtgaactac
     9001 tctttgcact attgcgagaa tactcttgtt tactgcttac ctgtttatta ctagctttag
     9061 ttaagtggga cttttgactc gctgttgttg tgtgcgtctt cgctaacacc tcactcgaaa
     9121 acaccagagt gagtaacata agaatcatcg ccccatatcg tcgtatggga gtcaaaatca
     9181 tcaggtctcc tggtcggatt taatcattcc aacaccttat atttttcaca aatttgagag
     9241 ttgaatctca aatcatatca aaaatagctg tcaagagcac cccaaggaat agtccaaatc
     9301 tgaaactatg tcacgagtta acgattcaga ttggcgctaa atcgcagaaa atgtgggggt
     9361 tatcgcaaaa ttcagccgtt ttttgcgcgc gatcgctcac ccttgcttct catcctgtgg
     9421 acttaccgct cagggatgag ttttgtctgg cttatcgctg gcaaactgtc tgaaatcgca
     9481 gcaataagga ctcatccacc atgaaactcg ccgcctgttt tctgacactc cttcctggct
     9541 tcgccgttgc cgccagctgg acttctccgg ggttccctgc ctttagcgaa cagggaacgg
     9601 gaacatttgt cagccacgcg cagttgccca aaggtacgcg tccactcacg ctaaattttg
     9661 accaacagtg ctggcagcct gcagatgcga taaaactcaa tcagatgctt tccctgcaac
     9721 cttgtagcaa cacgccgcct caatggcgat tgttcaggga cggcaaatat acgctgcaaa
     9781 tagacacccg ctccggtacg ccaacattga tgatttccat ccagaacgcg ccgaaccggt
     9841 agcaaacctg gtccgtgaat gcccgaaatg ggatggatta ccgctcacgc tggatgtcag
     9901 cgccactttc ccggaaggag ccgccgtacg ggattattac agccaacaaa ttgcgatagt
     9961 gaagaacggt caaataacgt tacaacccgc tgctaccagc aacggtttac tcctgctgga
    10021 acgggcagaa actgacacat ccgccccttt cgactggcat aacgccacgg tttactttgt
    10081 gctgacagat cgtttcgaaa acggcgatcc cagtaatgac cagagttacg gacgtcataa
    10141 agacggtatg gcggaaattg gcacttttca cggcggcgat ttacgcggcc tgaccaacaa
    10201 actggattac ctccagcagt tgggcgttaa tgctttatgg ataagcgccc catttgagca
    10261 aattcacggc tgggtcggcg gtggtacaaa aggcgatttc ccgcattatg cctaccacgg
    10321 ttattacaca caggactgga cgaatcttga tgccaatatg ggcaacgaag ccgatctacg
    10381 gacgctggtt gatagcgcac atcagcgcgg tattcgtatt ctctttgatg tcgtgatgaa
    10441 ccacaccagc tatgccacgc tggcggatat gcaggagtat cagtttggcg cgttatatct
    10501 ttctggtgac gaagtgaaaa aaacgctggg tgaacgctgg agcgactgga aacctgccgc
    10561 cggacaaacc tggcatagct ttaacgatta cattaatttc agcgacaaaa caggctggga
    10621 taaatggtgg ggaaaaaact ggatcagaac ggatatcggc gattacgaca atcctggatt
    10681 cgacgatctc actatgtcgc tggccttttt gccggatatc aaaaccgaat caactaccgc
    10741 ttctggtctg ccggtgttct ataaaaacaa aacggatact cacgctaaag tcatcgaagg
    10801 ctttacacct cgcgattact taacccactg gttaagtcag tgggtccgcg actatgggat
    10861 tgatggtttt cgggtcgata ccgccaaaca tgttgagttg cccgcctggc agcaactgaa
    10921 aaccgaagcc agctccgcgc ttcgcgaatg gaaaaaaact aaccccgaca aagcattaga
    10981 tgacaaacct ttctggatga ccggtgaagc ctggggccac ggcgtgatgc aaagtgacta
    11041 ctatcgccac ggcttcgatg cgatgatcaa tttcgattat caggagcagg cggcgaaagc
    11101 agtcgattgt ctggcgcaga tggatacgac ctggcagcaa atggcggaga aattgcaggg
    11161 tttcaacgtg ttgagctacc tctcgtcgca tgatacccgt ctgttccgtg aagggggcga
    11221 caaagcagca gagttattac tattagcgcc aggcgcggta caaatctttt atggcgatga
    11281 atcctcgcgt ccgttcggtc ctacaggttc tgatccgctg caaggtacac gttcggatat
    11341 gaactggcag gatgttagcg gtaaatctgc cgccaacgtc gcgcactggc agaaaatcag
    11401 ccagttccgc gcccgccatc ccgcaattgg cgcgggcaaa caaacgacac tttcgctgaa
    11461 gcagggctac ggctttgttc gtgagcatgg cgacgataaa gtgctggtca tctgggctgg
    11521 gcaacagtga cttttccggc ttcccgttcg tcagtacctc gggaagccgc caaccaggat
    11581 aaaatgtcag ccctaatcag cgttgcggga taaagcaccg ctcactcttc aacagaccga
    11641 tttgcacccc agcaaatgta gcgttattgt taccttcctt gctacagagt tcgacagata
    11701 tcccgctatg acattctccc tttttggtga caaatttacc cgccactccg gcattacgct
    11761 gctgatggaa gatctgaacg acggtttacg cacgcctggc gcgattatgc tcggcggcgg
    11821 taatccggcg cagatcccgg aaatgcagga ctacttccag acgctactga ccgacatgct
    11881 ggaaagtggc aaagcgactg atgcactgtg taactacgac ggtccacagg ggaaaacgga
    11941 gctactcaca ctgcttgccg gaatgctgcg cgagaagttg ggttgggata tcgaaccaca
    12001 gaatattgca ctaacaaacg gcagccagag cgcgtttttc tacttattta acctgtttgc
    12061 cggacgccgt gccgatggtc gggtcaaaaa agtgctgttc ccgctggcac cggaatacat
    12121 tggctacgct gacgccggac tggaagaaga tctgtttgtc tctgcgcgtc cgaatattga
    12181 actgctgccg gaaggccagt ttaaatacca cgtcgatttt gagcatctgc atattggcga
    12241 agaaaccggg atgatttgcg tctcccggcc gacgaatcca acaggtaatg tgattactga
    12301 cgaagagttg ctgaagcttg acgcgctggc gaatcaacac ggcattccgc tggtgattga
    12361 taacgcttat ggcgtcccgt tcccgggtat catcttcagt gaagggcgcc cgctatggaa
    12421 tccgaatatc gtactgtgca tgagtctttc caagctgggg ctgcctggct cccgctgcgg
    12481 cattatcatc gccaatgaaa aaatcatcac tgccatcacc aatatgaacg gcattatcag
    12541 cctggcacct ggcggtattg gtccggcgat gatgtgtgaa atgattaagc gtaacgatct
    12601 gctgcgcctg tctgaaacag tcatcaaacc gttttactac cagcgtgttc aggaaactat
    12661 cgccatcatt cgccgctatt taccggaaaa tcgctgcctg attcataaac cggaaggagc
    12721 cattttcctc tggctatggt ttaaggattt gcccattacg accgagcagc tctatcagcg
    12781 cctgaaagca cgcggcgtgc tgatggtgcc ggggcacaac ttcttcccgg gactggataa
    12841 accgtggccg catacgcatc aatgtatgcg catgaactac gtaccagagc cggagaaaat
    12901 tgaggcgggg gtgaagattc tggcggaaga gatagaaaga gcctgggctg aaagtcacta
    12961 aaaattgcct gatgcgctac gcttatcagg cctacatgat ttctgcaata tattgaattt
    13021 ggaagaattt gtaggccgga taaggcgttt acgccgcatc tggcataaac aaagcgcact
    13081 attccggcgt tgagaaacgc cggaaaacgt ttcaaacagg ctgctgctgt agccgatgcc
    13141 gctgcacttt ctcgacatcc atgcactgca acgcctttgt cgggcaggct tcaacacagg
    13201 ccggccccgt ctcccgatgc cagcacagat cgcacttaat cgccctcgcc tttttgcgcg
    13261 aagaaacgac ctccatcgca ccaaacgggc aagccagcat acagctttta cagccaatgc
    13321 aacgtgtttg ttcaacgaaa atatgcccat gttcgcggct tatcgcgtca acagggcaga
    13381 cattcgcgca cggtgcatct tcacactgat gacaggctac tggcgtggtc cagctgtggt
    13441 ctttaatgac acgaatacgg gaaataaact cgtctggtga caacgcagcg caatcctgat
    13501 tctcctgatg cgacactgcg caagccactt cacaggtacg gcaaccgata catttcgtcg
    13561 catccgcaat aataaaccgg ttcatgatca ttcctttgca ctaaaacagc agagtgccag
    13621 agagcgcgtc cctactgctt tgatccaacg aaggaaatgt taaagttttg gcacttgtcc
    13681 aaagcgttat gtgatcgcgc ccaggtcatc cctgacagta aatcccagtt cattagaaat
    13741 agcctgcgcg gtttcacgca gtggtttcag gagatttttc tctcccacct gtttcagacg
    13801 tgatgtcgaa agcgaaatcg acacggcgta cggcacccgc ccatgaatat caaacaccgg
    13861 aacagcaata caggagacgc cgagttcgtt ttcttctctg tccatcgccg ctccgctttc
    13921 acgaatgtgc gccagttcgt cgaacatcgc gggcagctcg gtaatggtat tgcgggttaa
    13981 cggctggatc tcatgctgat ggttttccca gtatgacttc acgtagtccg ggtgaccaaa
    14041 cgccatgtag atcttgccca ttgcggaaca gtagagcggc atatgctggc caatataggc
    14101 acgggttcgc agcatcccgg ttgtgggttc cagcttataa atcaaaatag cgtgatcgtc
    14161 ttcgcggctg gagaagttaa tggtttcacc agtggcgatg ttcagtgcct caagatgcgg
    14221 agcggcgata tgaatgatat tcagcgaaga cagcgccttc tgcccgacgg caataaattt
    14281 ggtagtcagg cggtaactcc ctgcggcggg cgcggtggtc acatagccgc aggactgtaa
    14341 tccctgcaat aagcgatgga cggtactctt atttaaacca gccagctccg aaagatgcgc
    14401 caacggacaa ccgtttggat agttgctcaa aatctcaatc agcatcaacc cgcgaaacag
    14461 actctggctt ccggctggac gctctttttc ctgcgccatc tcgttctctt tttttcccat
    14521 cacttctttc cccattttgt cgcgtcctga tggtagcgca aagtgtgccg tagttcacga
    14581 tctcgacaga taatttataa ccacttgatt tttatgtctt ttgaaattca tcaatcagat
    14641 tgctttgtta aaaagtgatc gatatattt
//
LOCUS       DAZXUK010000087        14458 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZXUK010000087 DAZXUK010000000
VERSION     DAZXUK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14458
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.087"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            3..548
                     /locus_tag="ACH3L8_002056"
     CDS             3..548
                     /locus_tag="ACH3L8_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4085179.1"
                     /translation="MAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEIHQDDIDK
                     EVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFDSSAKTTG
                     ADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFFAWMEQID
                     QATPVTPGAVTANATYVLDYK"
     gene            599..3058
                     /locus_tag="ACH3L8_002057"
     CDS             599..3058
                     /locus_tag="ACH3L8_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFT4085180.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3073..3804
                     /locus_tag="ACH3L8_002058"
     CDS             3073..3804
                     /locus_tag="ACH3L8_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFT4085181.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3801..4877
                     /locus_tag="ACH3L8_002059"
                     /pseudo
     CDS             3801..4877
                     /locus_tag="ACH3L8_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            5029..6075
                     /locus_tag="ACH3L8_002060"
     CDS             5029..6075
                     /locus_tag="ACH3L8_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFT4085182.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6072..6863)
                     /gene="nei"
                     /locus_tag="ACH3L8_002061"
     CDS             complement(6072..6863)
                     /gene="nei"
                     /locus_tag="ACH3L8_002061"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFT4085183.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6899..7633)
                     /gene="pxpA"
                     /locus_tag="ACH3L8_002062"
     CDS             complement(6899..7633)
                     /gene="pxpA"
                     /locus_tag="ACH3L8_002062"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFT4085184.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7623..8555)
                     /gene="pxpC"
                     /locus_tag="ACH3L8_002063"
                     /pseudo
     CDS             complement(7623..8555)
                     /gene="pxpC"
                     /locus_tag="ACH3L8_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(8549..9205)
                     /gene="pxpB"
                     /locus_tag="ACH3L8_002064"
     CDS             complement(8549..9205)
                     /gene="pxpB"
                     /locus_tag="ACH3L8_002064"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFT4085185.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(9228..9971)
                     /gene="ybgI"
                     /locus_tag="ACH3L8_002065"
     CDS             complement(9228..9971)
                     /gene="ybgI"
                     /locus_tag="ACH3L8_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFT4085186.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10242..11723
                     /gene="dtpD"
                     /locus_tag="ACH3L8_002066"
     CDS             10242..11723
                     /gene="dtpD"
                     /locus_tag="ACH3L8_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFT4085187.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11873..13291)
                     /gene="phrB"
                     /locus_tag="ACH3L8_002067"
     CDS             complement(11873..13291)
                     /gene="phrB"
                     /locus_tag="ACH3L8_002067"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFT4085188.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(13288..13797)
                     /locus_tag="ACH3L8_002068"
     CDS             complement(13288..13797)
                     /locus_tag="ACH3L8_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFT4085189.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            13926..14213
                     /locus_tag="ACH3L8_002069"
     CDS             13926..14213
                     /locus_tag="ACH3L8_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HFT4085190.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(14268..>14458)
                     /locus_tag="ACH3L8_002070"
     CDS             complement(14268..>14458)
                     /locus_tag="ACH3L8_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085191.1"
                     /translation="KFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELKTRLSTL
                     HVNLEQFINSSEDDEYPL"
BASE COUNT         3521 a         3860 c         3522 g         3555 t
ORIGIN      
        1 cattggccgc actggccgta tctctgctgt tcacagcacc tgtttatgcg gctgatgaag
       61 gatccggtga aattcacttt aaaggtgaag ttattgaagc accgtgtgaa atacatcagg
      121 atgatattga taaagaggtt gaactcggtc aggtgaccac cagccacatt aatcagtcac
      181 atcacagcga tgccgttgct gtcgacctgc tcttagtcaa ctgtgatctg gaaaactcca
      241 gcaacggttc cggtggcaag atttctaaag ttgcagtcac ctttgatagc tcagcgaaaa
      301 ccaccggcgc agatccgatt ctcaacaaca ccagcacggg tgaagccacc ggcgtcggcg
      361 tacgtttaat gaataaagat caaagcaata tcgttttagg caccgctact ccagatatcg
      421 acctggctcc gacctccagc gaacaaacgc tgaatttctt tgcctggatg gaacaaattg
      481 atcaggctac acctgtaacg ccaggcgcag ttacggcgaa tgcaacctac gtgctggatt
      541 ataaataagt attactttgg cggcgctatc gccgcctttc ataaaatatt tatcaggtat
      601 ggacaccgtg aatatttatc gactctcttt tgtatcctgc ctggtcgtgg cgatgccttg
      661 cgcattggcg gtcgaattca atctgaatgt tctcgataaa tcaatgcgcg accgcattga
      721 tatttcatta ttaaaggaaa aaggagtcat tgctcccggt gagtattttg ttagcgttgc
      781 ggtaaataat aaccaaatca gtaacgagca aaagattaac tggcacaaaa atgacgataa
      841 aaccattccg tgcatcaatg atttactggt cgataaattt ggcttaaaac ctgaagtccg
      901 tcagtcgtta ccattgataa atcagtgcgt cgattttagc tcccgacctg aaatgctctt
      961 caatttcgat caagccaatc agcaactgaa tattagtatt ccgcaagcct ggctggcgtg
     1021 gcactcagaa aactggaccc caccctccac atggaaagaa ggtgtcgccg gtgtcctgat
     1081 ggattacaac ttgtttgcca gcagctaccg cccacaggat ggcagcagca gcactaatct
     1141 taacgcctat ggaacagccg gaatcaacac gggggcatgg cgcttacgca gtgattatca
     1201 gttgaatcat actgatagcg atgataacca tgaacagtca ggcgaaatat cacgtaccta
     1261 tctttttcgc ccgttaccgc aattaggttc aaaattaacc ctcggcgaaa cggattttag
     1321 tcccaatatt ttcgacggtt tttcttatac cggcgcggca ctggcaagtg acgatcgaat
     1381 gttgccatgg gaactacgcg gctacgcccc acaaattagc ggtattgcac agaccaatgc
     1441 cacggtgacg atcagtcaat caggccgcgt catttaccag aaaaaagtcc caccaggccc
     1501 atttatcatt gacgacctta atcagtctgt tcagggcaca ctggatgtca aagtgacgga
     1561 agaagatggt cgggtgaaca atttccaggt ttcagcagca tcgacgccct tcctgactcg
     1621 tcagggacag gttcgctata aactggccgc gggtcagcca cggccctcca tgtcacatca
     1681 aactgaaaat gaaacctttt ttagcaatga agtttcctgg gggatgctgt caaacacctc
     1741 gctgtacagc ggcctgctgc tttctggtga tgactaccat tctgccgcaa tgggtattgg
     1801 gcaaaatatg ctgtggcttg gtgcgctgtc gtttgatgtc acgtgggcca gtagccattt
     1861 tgatactcag caggacgagc ggggcttaag ctaccgtttt aattacagca aacaagtgga
     1921 tgccactaac agcacgattt ccctcgccgc ttatcgtttc tccgatcgtc attttcacag
     1981 ctacgccaac tatctggatc acaaatacaa cgacagcgat gcgcaggacg aaaaacagac
     2041 gatcagctta tctgtgggcc aaccgattac cccactaaac ctcaatcttt acgccaacct
     2101 gctacatcaa acctggtgga atgcagacgc ctccacgacc gccaacatca cggcaggttt
     2161 taatgttgat attggtgact ggagagatat ctctatttct acgtcattca atacaaccca
     2221 ttacgaagat aaagatcgcg acaaccagat ttacctgtcg atttcgctcc ccttcggtaa
     2281 cggtggtcgg gttggctatg acatgcaaaa cagtagccac agcaccacac accgcatgtc
     2341 gtggaacgat acgctggatg aacgtaatag ctggggcatg tctgccggac tgcaatccga
     2401 ccgtcctgac aatggagccc aggtgagcgg taactatcag cacctgagtt cagcgggtga
     2461 gtgggatatt tctggtacct atgccgccaa tgattacagt tccgtcagca gcagctggag
     2521 cggttctttc accgcaaccc aatatggtgc agcgtttcat cgccgcagct ccaccaatga
     2581 accacgcctg atggtcagca ccgatggcgt ggcagatatt ccggttcagg gcaatctcga
     2641 ctacaccaac cattttggca ttgcggtggt gccgttgatt tccagttacc agccttccac
     2701 cgtggcggtg aacatgaatg acttacccga cggcgtaaca gttgcagaaa acgttatcaa
     2761 agaaacgtgg attgaaggcg cgataggtta caaatcactg gcttcccgtt ccggtaaaga
     2821 cgttaacgtc atcattcgca acgccagcgg tcagtttcct cccctcggag cggatatccg
     2881 ccaggatgac agcggcatta gcgtggggat ggttggcgag gaaggacatg cctggttaag
     2941 cggagtcgct gaaaatcaaa agtttaccgt ggtctggggt gatagccagc attgctcgct
     3001 ccatcttcct gaacatatgg aagacaccgc aaatcgcctg attttacctt gtcattaatt
     3061 aaggaaatga aaatgacatt tatgaaagga ctaccccttt tgttgttggt tgccagtctg
     3121 tgcagccacg ctgcactaca acccgatcgc actcgtattg tgtttaacgc taatgataaa
     3181 gctaccagtc tgcgggtgga taatcgtagc gataaactcc cctacctcgc ttattcctgg
     3241 ctcgaaaatg aaaaagggga gaaaagcgat gatcttctgg tcgccctgcc gccgatacaa
     3301 cgtctggaac cgaaagcaac aacacaggta cggatagtaa agcaagcctc gaccacaaaa
     3361 ttaccgggcg atcgcgaaac gctatttttc tacaatatgc gtgaaattcc tcccgccccg
     3421 gaaaaaaaca gcgaccatgc cgttctgcaa gtcgccattc agagtcgtat caaggtgttc
     3481 tggcgaccgg ctgcattacg caagaaagca ggagaaaagg tcgaactgca gttacaggtc
     3541 agccagcagg gcaaccaact gacactgaaa aatcctaccg cgtattatct gaccattgcc
     3601 tacctgggac gaaatgaaaa aggcgttctc cccggcttca aaaccgtgat ggttgcgcct
     3661 tttagtacgg tcaacacaaa caccggaaat tatagcggca gccagtttta tctcggttat
     3721 atggatgatt acggcgcgtt acgcatgacg acgctcaact gcagcggaca atgccattta
     3781 caagccgtgg aggcgaagaa atgagtgctg gcaagggagt gttgctcgtc atctgtctgt
     3841 tatttctgtc gttgaagtct gcgcttgccc tgaactgcta ctttggttca tccggtggct
     3901 cggtagaaaa atcagaagcg attcaacctt ttgctgtacc aggcaatgcc aaactcggcg
     3961 ataagatctg ggaatctgac gatattaaaa taagatctgg gaatctgacg atattaaaat
     4021 tcccgtctat tgtgacaaca ataccaacgg caactttgaa agcgaacatg tctacgtatg
     4081 ggtaaacccc tacccggggg tgcaggatcg ttattatcaa ctcggagtaa cttacaatgg
     4141 cgttgattat gacgccaacc agggaaaaag ccgcatcgac actaaccagt gtatcgacag
     4201 taagaacatt gacatttata cccctgatgg gatggcagaa caaaatttgc tcgggtgatc
     4261 ccgctaatat tcacatgtcg cgaacctttc tcgctcgcat gcggttatac gtcaaaatac
     4321 gagaaatgcc gccgcatgat tatcaaagta cgcttagcga ctatatcgtc gtgcaatttg
     4381 acggtgccgg tagcgttaac gaagacccta ctgcccaaaa cctgaaatat catattactg
     4441 gtctggaaaa cattcgcgtg ctggattgca gcgtcaattt ttccatttca ccggaaacac
     4501 agatgattga ttttggtaaa tttaatttgc tggatattcg tcgccacaca atgtcgaaaa
     4561 cattcagtat taagacgaca aaatcacaaa atgaccaatg caccgacgga tttaaagtca
     4621 gttcctcctt ttataccgaa gaaacgttgg ttgaagaaga taaagcattg ttgattggta
     4681 atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc ttataccttc aataaatatg
     4741 ctgaatacgc cgatttcacc agcgacatgt tggtctacga aaaaacctat acagctgaac
     4801 tttcgtccat tgcaggtacc cccatcgagg ctgggccctt cgatacagtg gtgcttttta
     4861 aaattaacta taactgattt gccatgatgc cctttgcagc cggaaagggc atctctttca
     4921 gaaaattgct gattttcccc ttttccttga ttgctgttat tttttcctag cccggctttc
     4981 tttatgctgt atcgatacac caaagagaaa ataatgaggg agcgaaggat gccagttttg
     5041 cagtggggaa tgttatgtgt gctatcactt ctcctttcta ttggtttcct cgcggtacat
     5101 ctcccggcgg cgctattact tgggccgatg atcgccggga tcatcttcag tatgcgcgga
     5161 attactctgc aacttccccg ctccgctttt ctcgccgcgc aggccattct tggctgcatg
     5221 attgcgcaaa acctcactgg ttcgattctc accacgctgg ccgccaactg gccgatcgtg
     5281 cttgctattt tactggtgac gctgctttcc agcgccatcg tgggttggtt attggtgcgc
     5341 tatagctcac tgcctggaaa taccggtgcc tggggttctt ctccaggcgg cgcagcggca
     5401 atggtcgcta tggcgcaaga ttacggcgca gatattcgcc tggtggcgtt tatgcaatat
     5461 ctgcgagtgc tgttcgtcgc gggggccgct gttctggtca cccgcatgat gctgggtgat
     5521 aacgctgaag cggttaacca gcaaatcgtc tggttcccgc cagtgagcat taatctcctg
     5581 cttacgattt tgctcgcagt cgttgccggt acggcgggat gcatgctgcg tcttccttcc
     5641 ggcacgatgc tcatcccgat gctggcgggc gcagtgctcc agtctggtca gctcatcacc
     5701 atcgaactac cggaatggct gctggcgatg gcgtatatgg caattggctg gcggattggt
     5761 cttggtttcg ataaacaaat cttactgcgg gcactacgcc cgctaccgca aatcctgctg
     5821 tcgatttttg ctctgctggc tatttgtgca ggtatggcgt ggggactgac ccggtttatg
     5881 catattgatt ttatgactgc ctacctcgcc accagtcccg gcgggcttga taccgtggcg
     5941 gtcatcgccg cagggagcaa tgccgatatg gcgcttatta tggcgatgca aaccctgcgc
     6001 ctgttcagta ttttgctgac ggggccagcc attgcgcggt ttatttcaac ctatgcgccg
     6061 aagcggtcgg cctagtgctg gcagcctgga caccagtaaa acgggcgcga ggacaacgtg
     6121 gttttctcaa tgatgccgcc acagcgttcg cacagttcgc catcacgatg aaaaacctta
     6181 aagcgaaaca gcgccccatg atgcttattc tcatccacct gcccccgcgt agcgtaagaa
     6241 aaccgaggaa tctccagtaa cgcgtgtgcg agtgcatcca gttgcgccgc attgagatct
     6301 ttcgctttat gatttccagt caacccaacc tgccagagga tctccacccg caaataattg
     6361 ccaagcccag ccagaaacgc ctgatcgagc agtaatcctg caaactgacg gttacgaaag
     6421 cgcggcgaca ataaccgttc ctttaccacc tccggcgtca gattcggatc cagcacatcg
     6481 ggaccaacgc gttgtaaaaa cggatgcgtg gtcagttgtt ccggcctcaa catctcaata
     6541 tccgaggcgc tataaagcag aatggttttg tcagccgttt gcagttttac ccgcaatact
     6601 cgcgtggtct gcggctcttc gccggtatca accacgcgcc agacgccgta aagctgatta
     6661 tggctgtaga gcgttaaacc gttgggaaaa tgagttaaca acgccttacc acgcgtttcc
     6721 acatgggtaa cgtgttgacc gataagttgt gattgataag tttttaactg cgggaaggca
     6781 aaccagacat cagttagtgg tttgcctttg atcgccgcct ccaggttatc cgctgcacgg
     6841 cggatctccg ggccttcagg catggtgata tccttttttc attctttatt aagcggggtt
     6901 atgctgcgac aacaatcccc tttttggcaa atgtagctcg taatcggcgg gcgaacgcca
     6961 gagcgtgctc gccgtcgcca tgcaggcaga ccgtttgcgc cgcgaccgtt gcccattcgc
     7021 cggtgatact tttgactctg ccgtgttgca ccatctccag cgtttgcgcc aacgcctgtt
     7081 cttcgttttc aatcaacgcg cctggctgac ttcgcggcac cagcgagccg tcagcctgat
     7141 aaccgcgatc ggcaaacact tcctcgcgcg ttgtcaggcc atattgcttg cctgcacgaa
     7201 tcagctcgct tcctgccagc ccaacgagaa ccaatgccgg atcgcaagcg tataccgctc
     7261 tggcgatggc gtctgccagt tgtgcttctt tcgccgcctg gttgtacaac atgccgtgcg
     7321 gtttgacatg acgcattacg ccgccttgcg cacgggcaat cgttgccagc gcgccaattt
     7381 gatacagcgt ctgggcgtaa acggtttccg gcggcagctg catggcgctg cgaccaaaat
     7441 tttccctgtc gggaaaactc gggtgagcgc caatcgcgac accatttttt attgcttcac
     7501 gcacgcaagc ctgcatggtt tgcgcatcgc ctgcatgaaa tccacaagca atattggcag
     7561 aggaaaccag cgttaatagc tctgcgtcgc tggcgcagcc ttcgcccaga tcggcgttca
     7621 ggtcaatttt cattgtgcag ccgccacgct aattgttcaa aataacgttg ctgatcttgc
     7681 cgcgctttca gtgcctcttc cagtgaacac tggacaaaat gaatcggctg accgagcgga
     7741 atttgcgcca gatggtacat atcagcctca atgatacagg caatacgtgg ataaccaccg
     7801 gtggtctgtg cgtcgttcat caacacaatc ggctgcccgt tatgcggcac ctgcaccacg
     7861 cccggtaaca aaccgtgaga taacagttcg cgatcggtga tgcgttttaa aatttgcccc
     7921 tgtaaacgat agcccattcg gttactttgc gagctaagct gccagggcga acgccagaat
     7981 gcatcctgcg aggcgcgatc gaactcatga tattccggcc ccggcaaggc gcgaatgcgg
     8041 ttgccccaca gcagctgttt aacgccctgc gcttccataa aatcacgctt cgatttgcca
     8101 atcgggagtc agtcaccatc cttcagtaaa cggccttcca gcccgccaat ccccactttg
     8161 agatcggtgc tgcatgaccc cattaccggc ggaacatcaa taccacccgc gaccgccaga
     8221 taactgcgca tcccgtgctg cgggcgttta agcgttaaac gctggcctgc tttcatcggc
     8281 aatcgccagc cggtccagac ggcgttatca tccaaccgcg cttcgcaacc tgcacccgtc
     8341 agggcaaacc acccatcagt ttcgaactca actgttaact gaccgagcgt gatctccagc
     8401 gcgggggcat tggcgtcatt acccaccagc aggttagcaa tgcgcagcgc aggcatatcc
     8461 agtgcgccac agtggctgat acccgactgg caaaaaccgt gacgaccgcc atcctgcaca
     8521 gtggtgtaca tgcccgcacg aataatcttc agcatattcc ctccttctgc ggtacaaatc
     8581 gcacgctgtc tcccggacgt aataagatgg gttcgtcacg cgccggatca aacaggctga
     8641 gtgaggtatg accaatcaac tgccagccac ccggcgttgc cagcggataa acaccagtct
     8701 gcggcccgcc gatcccgaca gaacctgccg gaacgagcaa gcgcggttca gcgcgccgtg
     8761 gcgtgtgtag ttgttccggc aaactcccga gatacgggaa gcccggttga aaacctaaaa
     8821 accagaccac gtattccacg gaggagtgca attcaacaac ctgtttttcg ctcaacccgc
     8881 aatacgccgc gaccaccgcc aaatccggtc cgcctgcacc accgtaaacc accggaattt
     8941 caataaagcg agactcaggt tccagggcct cgctctcctc ccaccagcgt tgcaaacgct
     9001 ctatggcatc cagcgccagc gactcaggat tacgcaaaat caccgtgata ttgttcatgc
     9061 cgggaatggc ttcaaccaca ttcggcatat ccaccagacg ctgcgccagt cgccagatcc
     9121 gtttctggct agccagcgtc accggcggtt ccagttccag cactaccgcc gtttcaccta
     9181 tcagataaca acgcgctcgt tgcactttcg atccctctga ttattcgtta tgcaggatta
     9241 ggaatatcaa taaaggtcac atcaagatcg gtattttcat tcagccactc gctcaatgcg
     9301 cgaataccac cgcgttcagt ggcatggtga cctgcagcat aaaaatgcaa tccctgctcg
     9361 cgggctgaat gaatggtctg ttcagaaact tcgccagtaa taaaagcatc cacgccaaaa
     9421 cgcgcggcgc tatcgataaa actttgcccg ccgcccgtgc accaggcgac gcgctggacc
     9481 acctcaggtc cggtatcgcc acaccataac ggcttacgtc ccagacgcgc ttcaatccag
     9541 gaggctagtt ccattcccgg cacaggcatg gtcagctctc cccacggcac caacggctca
     9601 atttcgccca tgaccgtgat ccccagtaac gccgccagct gtgcgttatt gcccagctca
     9661 ggatgtgcgt caagcggcaa atgccagcca tacaggttga tatcattcgc cagcaacgtt
     9721 tttaaacggt tacgcttcat tccgcgaatg accggagact ctcctttcca gaagtagcca
     9781 tgatgcacaa tgaccgcatc cgcgcccaga cgcactgcct catcgagcaa agcctggctg
     9841 gcggtgacac cggtaacaat tttttgcacc gtctctttgc cttccacctg caaaccattc
     9901 ggcgcgtaat cactaatcgc cgcgctgttc agtttttcgt taatcagttg ttccagttcg
     9961 gtgtttttca tcttcgcgcc ttccgtattc tgatagctac accgttaaca tagcgttaca
    10021 tcgcctgttc gtcgatagcc aatcttttga catattaaga aacaaacccg acgccaaaag
    10081 acgcaaattg tttatatcat cacgcttcag ctaatgtata tttatgcaat cagacagcat
    10141 attgcataaa taatcagatc tgtatcaaag ttttaacttt aaggaaacac aagcacgccg
    10201 cgcaactatt acgtgacgtt gccgtcaatt gtggaattat tatgaataaa cacgcatcac
    10261 aaccgcgcgc tatttactat gtcgttgcgc tgcaaatctg ggaatatttt agcttttacg
    10321 gcatgcgtgc cctgctgatt ctctatctca ccaatcaact aaaatacaac gatactcacg
    10381 cctacgagtt atttagcgcc tactgttcgc tggtgtatgt cacgccaatc ctcggtggct
    10441 ttttggcgga taaagttctc ggcaatcgca tggcggtgat gctgggggcg ttgttgatgg
    10501 cgatcggtca tgtggtgctg ggtgccagtg agatccatcc gtcattcctc tatctgtccc
    10561 tggcgattat cgtctgcggc tatggcctgt ttaaatctaa cgtaagctgt ctgctcggcg
    10621 agctgtatga gccaaccgat ccgcgtcgtg atggcggttt ctcgctgatg tatgcggcgg
    10681 gtaacgtggg gtctattatc gcacctatcg cctgtggtta cgcccaggaa gagtacagct
    10741 gggcgatggg ctttggcctg gcggcggttg gaatgatcgc gggtctggtc attttcttat
    10801 gtggcaatcg tcatttcact catacccgcg gcgttaacaa aaaagtactg cgtgcgacaa
    10861 actttctcct gccgaactgg ggatggctgc tggttctgct ggtggcaacg cctgcactga
    10921 ttaccgtgct gttctggaaa gagtggtcgg tatacgcctt aattgtcgcg actattattg
    10981 gcctgggtgt actggcaaaa atttatcgca aagcagaaaa ccagaaacag cggaaggagc
    11041 tggggctgat tgtgacgctc accttcttca gtatgttgtt ctgggccttc gcacaacagg
    11101 gcggtagctc gattagcctt tatatcgacc gcttcgttaa ccgcgatatg tttggttata
    11161 ccgttccgac cgcgatgttc cagtcgatta atgccttcgc agttatgctg tgcggtgtgt
    11221 tcctggcgtg ggtggttaaa gaaagcgtcg caggtaatcg taccgtgcgc atctggggaa
    11281 aatttgctct tggccttggc ctgatgagcg ccggattctg cattctgacc ttaagcgccc
    11341 gctggtccgc aatgtatggt cactcttctc tgccactgat ggtgttaggc ctcgcggtga
    11401 tgggctttgc ggaactgttt atcgacccgg ttgccatgtc gcaaattacg cgcattgaaa
    11461 ttccgggcgt gaccggcgta ttaaccggca tctacatgct gctttctggc gcgatcgcga
    11521 actatctggc gggcgtgatt gccgatcaga catcgcaggc ttcgtttgat gcttccgggg
    11581 cgatcaacta ctccatcaat gcatatattg aagtatttga tcaaattacc tggggcgcac
    11641 tggcgtgtgt aggactggta ctgatgattt ggctgtatca ggcgctgaaa ttcagaaacc
    11701 gcgcgctggc gctggagtca taataggcca gcaccgatgc ctgatgcgac gcttgacgcg
    11761 tcttatcagg cctacgtatt tcctgcaatt tattgaattt gcacaaattt gtaggctgga
    11821 ttaggcgttc acgccgcatc cggcatctgg gctcgattgc ctgacgcgtc tgttatttcc
    11881 ccttccgcgc cgcctcatac gctgccaacg tttgtactct cgcctctttg tgctcgacta
    11941 tcggttgcgg ataatccagc ttcacacctg ctttctgcgc ccacttccac ggctcatgca
    12001 ccgctttccc tggtacatcg cgcagctccg gtaaccatcg acgaataaac tcgccctcac
    12061 ggtcaaattt ctcgccctga gttatcggat tgaaaatacg aaaatacggc gcagcatcag
    12121 tacccgtaga agccgcccac tgccagccac cgttattagc tgccaaatca ccatcaatca
    12181 gctgcgacat gaaatatcgc tcgccttcgc gccagtcgat caacaaatct ttcaccagaa
    12241 aactggctgt aatcatccgt agccggttat gcatccagcc agtgctgtta agctgacgca
    12301 tagcggcatc aataatcggg tatcccgttt tgcctttctg ccaggcctgt aaatgtgcgg
    12361 gattgctttg ccactgcaca cgatccgtcc aggcaataaa tggacgatgt ttacacaacg
    12421 aggggtaata cgtcatcaga tgacggtaga actcgcgcca gatcagctca ctaagccaga
    12481 cactaccggc cccaccgtcc agcgcctgcg gctgttccgc caacaagcga tgcaaacact
    12541 ggcgaggcga taacccgccc gttgccaagc tggcggacaa acggctggtg ccttccactg
    12601 ccggaaaatc tcgttgttgc tcatactctc cagcaccgtt ctcgcaaaac tggcgtaatt
    12661 gcgcaatcgc cgctttttct tccaccggga aatgcgcagt atcgaaagac tgacgaggat
    12721 aattcagcgt aatggatggc gcgggcttta tcgatccgct actacgaact tttggtgcag
    12781 cgacgcactc cggcatccct tcccgcagcc gtttcagcca ggcattctta aaaggcgtaa
    12841 agactttgta catctcgtga ttgccggtca tcaccgcccc cggcggcagg atcacgctgt
    12901 catcaaatcc ttcacacacc acgttacgca gcgctctttc aacgtccaca tcccgcgctc
    12961 gctcattcac ctcatactga tagttataaa aaagatgagt aacgctgttt tccgcgcaca
    13021 cctgtttaac tatttcgaca ctggcgacaa agtcatccac ttcacggaac aataaaggaa
    13081 tacctttttc cgccagcgcg atttgtagcc cattcagttg agcattgata agttcagcct
    13141 gacgcggcga catgttatgc gtctcccact tgcgtggtgt agcgatatac agcgccagca
    13201 cgcgtgcaga cgaattgcgg caggcggcag ccagtgcgag attatcgtgc agacgtaaat
    13261 cctggcgaaa ccaggccaga tgggtagtca taaaactcct gaatggcgca agttaatcac
    13321 acgcggccaa agttcgaaat aacgctgccc tgaaagccag gcgtcaggat aaagcgccat
    13381 atagtgtttg atacgcatca gcggcgcaag aagtggttgc tcgccacggc gataactgtc
    13441 gataagcgca gccagctgct ggcgttctgt ggaatcaata tgcgggcgaa aataaccctg
    13501 aacgtgcatc aaaacgttgg tgtgatcgcg cgggttggcg ggatgagaaa gcaaaacaat
    13561 tacgcgttgg cggtactggt tgtaaaaatc atccagattt tgccactgat gaatatcagc
    13621 cacaaacggg ccaagtttgc ggtactcggg ctgagaatgg gccagaaaga cgagtttata
    13681 gcggctgtga taatcgagaa gcgccccgcg cgttaagcca tgttctttca gtcgatgtaa
    13741 ctcatgaagg gcgaagatac ggattagggt gctgtcatca aatcgttgta gattcattac
    13801 tgctcctgaa cggtatacag gagtaagtgt aggcgttggt aaatgatgtt tgtgaagtta
    13861 atccgctagc agggcaagat tacgaaagcg cgctccccgc aagcaactaa tcaaggtagt
    13921 taatcatggc tgaacacgat attacgccag aagtcactat ttccaaaaca cagcgccgct
    13981 ataaagtcgg ctatgtcaga gcccgccacc aggaccgcaa aaccggtata gcccggtatt
    14041 attcacaaca tcccagtctg caccttaagg gcgactggct gaaagaggcc ggatttgata
    14101 ccggacgggg tgtcacggtg aagatttcgg aggggggcct gaccatcatc gctgacagtg
    14161 acgaggtgca ggaactgcgg gatgggatgt ttagtgtgct gaacgaaagt taaatattaa
    14221 aaaagctggg aagatttaaa accttcctag ctttttattc tgtcttatta taatggatac
    14281 tcatcatctt ccgatgaatt tataaattgc tccaagttta catgaagagt acttaaccta
    14341 gtttttaact cattaagtga agcgataatt tcatattttc caacataatc atatgatgga
    14401 tcgtattcat tatctatgtc agaagctaaa aatgatgata ttcgtagttt aaatttag
//
LOCUS       DAZXUK010000088        14407 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZXUK010000088 DAZXUK010000000
VERSION     DAZXUK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14407
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.088"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            232..1290
                     /gene="citC"
                     /locus_tag="ACH3L8_002071"
     CDS             232..1290
                     /gene="citC"
                     /locus_tag="ACH3L8_002071"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFT4085192.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1305..1601
                     /gene="citD"
                     /locus_tag="ACH3L8_002072"
     CDS             1305..1601
                     /gene="citD"
                     /locus_tag="ACH3L8_002072"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFT4085193.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1598..2506
                     /gene="citE"
                     /locus_tag="ACH3L8_002073"
     CDS             1598..2506
                     /gene="citE"
                     /locus_tag="ACH3L8_002073"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFT4085194.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2517..4049
                     /gene="citF"
                     /locus_tag="ACH3L8_002074"
     CDS             2517..4049
                     /gene="citF"
                     /locus_tag="ACH3L8_002074"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFT4085195.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4053..4603
                     /gene="citX"
                     /locus_tag="ACH3L8_002075"
                     /pseudo
     CDS             4053..4603
                     /gene="citX"
                     /locus_tag="ACH3L8_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4578..5456
                     /gene="citG"
                     /locus_tag="ACH3L8_002076"
     CDS             4578..5456
                     /gene="citG"
                     /locus_tag="ACH3L8_002076"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFT4085196.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5507..6969
                     /gene="citT"
                     /locus_tag="ACH3L8_002077"
                     /pseudo
     CDS             5507..6969
                     /gene="citT"
                     /locus_tag="ACH3L8_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7083..7889
                     /gene="rna"
                     /locus_tag="ACH3L8_002078"
     CDS             7083..7889
                     /gene="rna"
                     /locus_tag="ACH3L8_002078"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFT4085197.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8118..8528
                     /gene="rnk"
                     /locus_tag="ACH3L8_002079"
     CDS             8118..8528
                     /gene="rnk"
                     /locus_tag="ACH3L8_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFT4085198.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8666..8755
                     /gene="yldA"
                     /locus_tag="ACH3L8_002080"
     CDS             8666..8755
                     /gene="yldA"
                     /locus_tag="ACH3L8_002080"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFT4085199.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8759..9997)
                     /locus_tag="ACH3L8_002081"
     CDS             complement(8759..9997)
                     /locus_tag="ACH3L8_002081"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFT4085200.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10218..10646
                     /gene="uspG"
                     /locus_tag="ACH3L8_002082"
     CDS             10218..10646
                     /gene="uspG"
                     /locus_tag="ACH3L8_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFT4085201.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10767..12332)
                     /gene="ahpF"
                     /locus_tag="ACH3L8_002083"
     CDS             complement(10767..12332)
                     /gene="ahpF"
                     /locus_tag="ACH3L8_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFT4085202.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12577..13140)
                     /gene="ahpC"
                     /locus_tag="ACH3L8_002084"
     CDS             complement(12577..13140)
                     /gene="ahpC"
                     /locus_tag="ACH3L8_002084"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFT4085203.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13512..14258
                     /gene="dsbG"
                     /locus_tag="ACH3L8_002085"
     CDS             13512..14258
                     /gene="dsbG"
                     /locus_tag="ACH3L8_002085"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFT4085204.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3473 a         3798 c         3593 g         3543 t
ORIGIN      
        1 ttaattaaaa aacttaaaac cattaaataa ataaaaaacc actaactcta tgtgaaataa
       61 atcaaaattt cacgccgaaa tactccttag gatgtatagc gaaaggagaa aaagatatac
      121 ctcgatcacc cccctttctc ccaagtgaaa ataaaaggtt atcagtttgc aacattgaac
      181 aacattcgtt gcaaatcgat aacaacatgc accttcagga tactatttat tatgttcggc
      241 aatgatattt tcacccgcgt aaaacgttca gaaaataaaa aaatggcgga aatcgcccaa
      301 ttcctgcatg aaaatgattt gagcgttgac accacagtcg aagtatttat taccgtaacc
      361 cgcgatgaaa agcttatcgc gtgcggtgga attgccggaa atattattaa atgcgttgct
      421 atcagtgaat ccgttcgcgg tgaaggactg gcgctgacat tagccactga actgataaac
      481 ctcgcctatg agcggcacag cacgcatctg tttatttata ccaaaaccga atacgaggcg
      541 ctgttccgcc agtgcggttt ttccacgctg acctgcgtac ccggcgtgat ggtgctgatg
      601 gaaaacagcg ccacgcgact gaaacgctat gccgaatcgc tgaaaaaatt tcgtcatcca
      661 gggaacaaga ttggctgcat tgtgatgaac gccaatccct ttacgaatgg tcaccgttat
      721 ctgattcaac aagctgcggc acagtgcgac tggttgcatc tgtttttagt caaagaagat
      781 tcttcacgct tcccctatga agaccggctg gatctggtgt taaaaggcac cgccgatatt
      841 ccacgcctga ctgtgcatcg cggctcagaa tacatcatct cccgcgctac gttcccttgc
      901 tacttcatta aagaacagag cgtcattaac cattgttaca ccgaaattga tctgaagatt
      961 ttccgtcagt acctcgctcc cgcgcttggt gtaactcacc gctttgtcgg tactgaaccc
     1021 ttttgtcgcg ttaccgccca gtacaaccag gatatgcgct actggctgga aacgccgact
     1081 atctccgcag cgcccatcga actggttgaa attgagcggc tgcgttacca ggagatgccg
     1141 atatccgctt cccgggtacg tcaactgctg gcgaaaaacg atctcacggc tatcgcgccg
     1201 ctggtccctg cagtcacgct gcattatttg cagaacctgc ttgagcactc ccgccaggac
     1261 gcggcagctc gtcaaaagac ccccgcatga gaaacaggtg aaaaatgaaa ataaaccagc
     1321 ccgccgttgc aggcaccctt gagtctgggg atgtgatgat acgcatcgcc ccactcgata
     1381 cgcaggatat cgacctgcaa atcaatagca gcgttgagaa acagtttggc gatgcaattc
     1441 gcaccaccat tctggacgtt ctcgcccgct acaacgtgcg cggcgtacag ctgaatgtcg
     1501 atgacaaagg cgcactggac tgcattttac atgcacgact ggaagccctg ctggcacgcg
     1561 ccagcggtat cccggctctg ccatgggagg attgccaatg atttccgctt cgctgcaaca
     1621 acgtaaaact cgcacccgcc gcagcatgtt gtttgtgcct ggtgccaatg ccgcgatggt
     1681 cagcaactcc ttcatctacc cggctgatgc cctgatgttt gacctcgaag actccgtggc
     1741 attacgtgaa aaagacaccg cccgccgcat ggtttatcac gcgctgcaac atccgctgta
     1801 tcgcgatatt gaaaccattg tgcgtgtcaa cgcgctggat tccgaatggg gtgttaacga
     1861 cctggaagcc gtcgttcgcg gtggtgcgga cgttgtgcgt ctgccgaaaa ccgataccgc
     1921 tcaggatgtt ctggatattg aaaaagagat cctgcgtata gaaaaagcct gtggtcgtga
     1981 acccggcagc accggcctgc tggcggcgat tgaatctccg ctgggcatta cccgcgcagt
     2041 ggaaatcgct cacgcttccg agcgtttgat cggtatcgcc ctcggtgcag aagactatgt
     2101 gcgcaacctg cgtacagaac gctccccgga aggaactgaa ctgctgttcg cacgctgttc
     2161 cattttgcag gccgcgcgct ctgcgggtat tcaggcgttc gataccgtct attccgacgc
     2221 taacaacgaa gccggattcc tgcaagaagc cgcccacatc aaacagctgg gctttgacgg
     2281 caaatcgctg atcaacccgc gtcagattga tctgctgcac aacctctacg caccgaccca
     2341 gaaagaagtg gatcacgccc accgcgtcgt agaagccgct gaagccgccg ctcgcgaagg
     2401 cctcggcgtg gtttccctga acggcaagat ggtggacggt ccggttatcg atcgcgcccg
     2461 tctggtgctc tcccgtgcag aactttccgg catccgcgaa gaataaggca atcaaaatga
     2521 cgcagaaaat tgaacaatct caacgacaag aacgggtagc ggcctggaat cgtcgcactg
     2581 aatgcgatct tgccgctttc cagaactcac caaagcaaac ctaccaggct gaaaaagcgc
     2641 gcgatcgcaa actgtgcgcc aacctggaag aagcgattcg tcgctcaggt ttacaggacg
     2701 gcatgacggt ttccttccat cacgctttcc gtggcggtga cctgaccgtc aatatggtga
     2761 tggacgtcat cgcgaagatg ggctttaaaa acctgaccct ggcgtccagc tccctgagtg
     2821 attgccatgc gccgctggta gaacacattc gccagggcgt ggttacccgc atttatacct
     2881 ctggcctacg cggtccactg gcggaagaga tctcccgtgg tctgctggcg gaaccggtgc
     2941 agatccactc tcacggcggt cgtgtgcatc tggtacagag cggcgaactg aatatcgacg
     3001 tggctttcct cggcgtcccg tcctgtgatg aattcggtaa tgccaacggc tacaccggta
     3061 aagcctgctg cggctccctc ggctatgcaa tggttgatgc cgacaacgca aaacaggtcg
     3121 tgatgcttac cgaagaactg ctgccttatc cgcataatcc ggcaagcatt gagcaagatc
     3181 aggttgattt gatcgtcaaa gttaatcgcg ttggcgatgc tgcaaaaatc ggcgctggta
     3241 ctacccgtat gaccactaac ccgcgcgaac tgcttattgc ccgtagcgct gcggatgtga
     3301 ttgtcaactc tggctacttc aaagaaggtt tctccatgca aaccggcacc ggcggcgctt
     3361 cgctggcggt aacccgtttc ctggaagaca aaatgcgtag ccgcgatatt cgcgccgact
     3421 tcgccctcgg cggtattacc gcgacgatgg ttgatctgca cgaaaaaggt ctgatccgca
     3481 aactgctgga tgtgcagagc tttgacagcc atgctgcgca atcgctggcc cgtaacccca
     3541 atcacatcga aatcagcgcc aaccagtacg ctaactgggg ttcgaaaggc gcatcggttg
     3601 atcgtctcga cgtggtggta ctgagcgcgc tggaaattga caggcagttc aacgttaacg
     3661 tgctgaccgg ttctgacggc gtactgcgtg gcgcttccgg tggtcactgc gataccgcga
     3721 ttgcctctgc gctttccatc atcgtcgcgc cactggtacg cggtcgtatt ccgactctgg
     3781 tggataacgt gctgacctgc atcaccccgg gctccagtgt cgatattctg gtcacagacc
     3841 acggtatcgc agttaacccg gcacgtccgg aactagcaga acgtctgcag gaagcgggca
     3901 ttaaagtggt ttccattgag tggctgcgcg aacgtgcgcg tctgctgacc ggtgaaccac
     3961 agccgattga attcaccgac cgcgtcgttg ccgttgtgcg ttaccgcgat ggctcggtga
     4021 tcgatgttgt gcatcaggtg aaggaataag ccatgcacct gcttcctgaa ctcgccagcc
     4081 accatgcggt gtcaattccc gagctgctcg tcagccggga tgaaaggcaa gcacggcaac
     4141 acgcctggct caagcaccat cctgttccac tggtctcctt taccgtggtt gcgcctgggc
     4201 cgattaaaga cagcgaggtc acacgccgaa tttttaatca tcgcgtgaca gccttgcgtg
     4261 ccttagccac aaagcagggc tggcaaattc aggagcaggc tgcactggtt tccgccagcg
     4321 ggccggaggg catgttgagc attgccgccc cggctcgcga cctcaagctc gccaccattg
     4381 agcttgaaca tagtcatcct ctcgggcggt tatgggatat cgatgtcctg acgcccgaag
     4441 gcgaaattct ctcccgccgc gactattcac tgcgcctcgc cgctgcctgt tgtgcgagca
     4501 aagcgcagcc gtctgcgcgc gtggaaaaac ccatcaactg accgatttac tcaaccgcat
     4561 ggaggcactg ctgaacgatg tcgatgcctg caacgtcaac taaaaccaca aagcttgcga
     4621 cgtcattaat cgatgagtac gccctgctgg gctggcgcgc catgctgact gaagtcaatc
     4681 tgtcaccgaa accaggcctc gtggatcgca ttaactgcgg tgcgcacaaa gatatggcgc
     4741 tggaagattt ccaccgcagc gcgctggcga ttcagggctg gttaccccgt ttcattgaat
     4801 ttggtgcctg tagtgcagaa atggcaccgg aagcggtact ccacggatta cgcccaattg
     4861 gtatggcttg cgaaggtgat atgttccgcg ccactgcggg cgtaaacacg cataaaggca
     4921 gcattttttc tttagggctg ctatgtgcgg caattggccg tttgcttcag ctcaaccagc
     4981 cggttacgcc aacaaccgtt tgttctacgg cggcaagttt ctgccgtggc ctgaccgatc
     5041 gcgaactgcg taccaataat tcacgactga cggcaggtca acggttgtac cagcagcttg
     5101 gccttaccgg cgcacgcggt gaagccgaag caggttatcc actggtgatc aaccacgcct
     5161 tgccgcatta cctcactctg ctggatcagg ggttagatcc tgaactggca ttgctcgata
     5221 ccttgctcct actgatggct accaacggcg ataccaacgt tgcatcgcgc ggtggcgagg
     5281 ggggcctgcg ctggctacag cgcgaggcgc aaacattatt gcaaaaaggg ggcattcgaa
     5341 cccccaccga tctcgattat ctccggcagt tcgacaggga gtgtatcgaa cgaaatctca
     5401 gtccaggcgg cagtgccgac ctgctgatcc ttacctgttt tttagcacag atttaattat
     5461 ttaagcactt gataaatttg gaaatattaa ttttcggaga acccgtatgt ctttagcaaa
     5521 agataatata tggaaactat tggccccact ggtggtgatg ggtgtcatgt ttcttatccc
     5581 tgtccccgac ggtatgccgc cgcaggcatg gcattacttc gctgtgtttg tggcaatgat
     5641 tgtcggcatg atcctcgagc caattccggc aacagcgatc agttttattg cggttactat
     5701 ttgcgttatt ggcagtaatt acctgctctt tgatgccaaa gaattagctg acccagcgtt
     5761 taatgcgcaa aaacaggcgc tgaaatgggg cctggctggt ttttccagca ccacggtatg
     5821 gctggtattt ggcgcattta tttttgcatt agggtatgaa gtttccgggt taggtcgtcg
     5881 cattgccctt ttcctggtga aattcatgga caaacgcacg ctgacgttgg gttatgcgat
     5941 tgtcattatc gacattctgc tggcaccgtt tacaccgtcc aacaccgcgc gtaccggggg
     6001 tacggttttt ccggtcatta aaaacctgcc gccgttgttt aaatcattcc cgaacgatcc
     6061 gtccgcgcgt cgtattggcg gctatttgat gtggatgatg gtcattagta ccagtctgag
     6121 ttcgtccatg tttgtcaccg gtgcggacca aacgtgctgg gtctggagtt cgtcagcaaa
     6181 attgccggta tccagattag ctggttgcag tggttcctct gcttcctgcc ggttggggtt
     6241 atcttgctta tcattgcgcc gtggctttcc tacgtgctgt acaaaccgga aatcacacac
     6301 agtgaagaag tggcaacctg ggcgggtgat gaactaaaaa ccatgggtgc gctgacacgc
     6361 agagagtgga cgctgattgg ccttgtattg ctcagcttag gtttgtgggt atttggcagt
     6421 gaagtcatta atgctactgc ggttggtctg ctggcagttt cgctaatgct ggctctgcac
     6481 gttgtgccgt ggaaagacat tacccgctat aacagcgcat ggaacacgct ggtcaacctg
     6541 gcaactctgg ttgtgatggc taacggcctg actcgttctg gttttattga ctggttcgcc
     6601 ggtaccatga gtacgcacct ggaaggattc tcaccaaacg caacggtgat tgtactggtt
     6661 ctggtgttct actttgcaca ctacctgttt gccagcctgt ctgcgcacac cgcaaccatg
     6721 ctgccggtta ttctggccgt cggtaaaggt attccgggcg taccaatgga acaactgtgt
     6781 atcctgctgg tgctgtctat cggtatcatg ggctgtctga cgccgtatgc aaccggtcct
     6841 ggggtgatta tttacggctg tggctatgtg aaatcaaaag attactggcg tcttggcgca
     6901 atcttcgggg tgatttacat ctctatgttg ctgttggttg gctggccgat tctcgccatg
     6961 tggaactaat acgttcgcca aacgctgaat aaatcatcac gcccgccagg taagcccacc
     7021 tggcgggctt ttttatgatt taatagatag tattcagtgt atgtatgagt tccacaccca
     7081 ttatgaaagc attctgccgt aacgccgcgt tgctcgcggt ttctctgctt cccttatctt
     7141 ctgccaacgc cgtagcgttg caggcaaaac agtatggcga ttttgatcgc tatgtcctgg
     7201 ccctctcctg gcaaaccgga ttttgccaga gtcaacacga tcgaaatcgt aacgaacgag
     7261 atgaatgtcg cctgcaaacc gaaacgacca acaaaactga ttttctgacc gtacatggtc
     7321 tgtggccagg attgcctaaa tcggttgctg cccgtggtgt tgatgaacgc cgctggatgc
     7381 gcttcggttg cgctactcgc ccaatcccga atctaccaga agcacgcgcc agccgaatgt
     7441 gttcatcgcc ggaaaccggg ttatcactgg aaacggccgc taaactaagt gaagtcatgc
     7501 caggagctgg cggacgttcc tgcctggaac gctacgaata tgccaaacac ggtgcctgct
     7561 ttggttttga tccggacgca tacttcggta cgatggtacg cctgaatcaa gaaattaaag
     7621 agagcgaagc cggaaaattc cttgcggata attacggtaa aacagtgagc cgccgtgact
     7681 ttgacgccgc ctttgccaaa agctggggaa aagagaacgt gaaagcagtt aagctaacgt
     7741 gccagggtaa ccctgcgtat ttgactgaaa ttcagatctc gatcaaagct gacgccatca
     7801 acgctccgct ttctgcaaac tcattcttgc cacaacctca cccaggtaac tgtggcaaaa
     7861 cctttgtgat tgataaagcg ggttattaat cctctgattt acaaaacacg ctaattcgtc
     7921 ttccataacc atgccgatta agaattatac ataatgcgct gcattgagga attccctgaa
     7981 tgtgacgcaa atcacttcaa agagtgagca acagtcagta tcatcatgtg agttaaaccc
     8041 tcgccgcctg acggtgaggg ttttcttttg ggtatgttta tctgcgacac tcgcagtacc
     8101 gacgacatgg agtaaaaatg tccagaccaa ctatcatcat taacgacctg gatgccgaac
     8161 gcatcgatat tctgctggag caacccgcct atgctggttt gccaatcgcc gacgcgttaa
     8221 acgcagagtt ggatcgcgcc caaatgtgtt cgccagaaga gatgccacac gacgtggtga
     8281 caatgaacag ccgggttaaa ttccgcaatc ttagcgatgg cgaagtgcgt gtgcgcacgc
     8341 tggtgtatcc ggcaaaaatg accgatagca atactcagct ttccgttatg gctccggtag
     8401 gtgccgcact gctggggctg cgcgttggcg attccattca ctgggaactt ccgggcggcg
     8461 ttgcaaccca ccttgaagtg ctggaactcg aataccagcc agaagctgct ggcgactacc
     8521 tgctttaatc ccatctgcac aagcgataac tcgctttgcc cagaggatgc actcgcatcc
     8581 tctttacgca aaatcagcaa tttttcctgg ttctcactgt tcacttttgc tgcaaaatta
     8641 ttccgtttcg tttgtgggaa taaccatggc agaagcattt tacatcctga taggatttct
     8701 gattatggcg gcgattatcg tcatggctgt cctttacctc gaaaaccact cctgacgatc
     8761 atattgttcc ccccggcatc gcattcacca gacctgaaac cgccttctgc gccgcctctg
     8821 cgctttgtgc accgggtacc agaatcacct tacggcactc ctcttcacgt ttttcgaaaa
     8881 tctcatatcc ccgggcggcc tcttcaaacg gcatatagtg ggtaacaatt tcttctggtt
     8941 tcagtaatcc tttctcaatt aacggtaata attctcccag ccatgcgtga acgtgggtct
     9001 gtcccatttt aaacgtcaac cctttatcaa aggcgtcgcc aaacaggaaa ccgtgaataa
     9061 atccagcgta gacgcccggt acgctaacaa tgccgccacg cctgaccgcc gcaatacact
     9121 ggcgcaacgc tttaccgctg ctgccctcca gtttcaggtt agtcagcacc gtttccgtgg
     9181 tgctgccttt cgcttcaaaa ccgacggcgt ctattactgc atccacgccc cggtgacctg
     9241 ccgtttgttc aataattgac tgtgccggat cgctatcttc atcgaaatta atcgggatcg
     9301 cgccgtagcg gtcagcggcg aaacgcaagc ggtagggatg atgatcaaca acaaaaatct
     9361 gttccgcacc gagcaaccgt gcacaggcga ttgtcaacaa tcccacagga ccagcaccat
     9421 agactgcaac gcttgaacct tgttggatct gcacattttt tgctgcctgc catgccgttg
     9481 gcagaatatc agaaaggaaa agggctttat catctgaaag caaaggcggt actttaaacg
     9541 gccccacatt ccctttcggg acgcggacat attccgcctg cccgccagga acgccaccat
     9601 acaggtgact ataaccaaac aatgccgcgg gagcaggtat ctgtttttta ttgagcgcag
     9661 cgcctttacc cgcattggta ttttcgcagg cggcatattg ctgtaatcga cagaaaaaac
     9721 agtcgccaca agcaatgacg aacggaatta ccacccggtc gcctttttgc aaatttttta
     9781 cgtccttccc ggtttcaacg acttccccca taaattcatg accaaaaata tcgccatgtt
     9841 taacctgggg tattttgcct cgataaagat ggaggtcaga gccacagatc gccgttaccg
     9901 taatacgcag aataatatca tctgcctgtt caatgcccgg atcgggaaca ttttctacct
     9961 gaacgtgatg tgggccgtga taagtcaatg ctttcatgca acctccagag acagttcaaa
    10021 aaagagtcag tctttaaggg tagctatcac agcgggaaaa cgggcggtac cgcaggtttt
    10081 gggagaattc ctagtgttca gtgagaatat attaatcaca gaaaccgttc gtaaggaaaa
    10141 agaaccagac aaatatcagg gataaatatt ataatcatgg agaatgattt gttcatgatt
    10201 aacagggaga aagggttatg tataaggcaa tcattatgcc agttgatgta tttgaaatgg
    10261 aattgagcga caaagctgtt cgccacgctg aattcctcgc ccaggatgac ggagttattc
    10321 atctacttca cgtactaccc gggtcagcca gcctgagcct gcaccgtttt gccgctgatg
    10381 tgcgtcgttt tgaagagcat ctgcaacatg aagcagaaga acgtctgcaa acgatggtca
    10441 gccacttcac catcgatcct tcccgcatta aacaacatgt ccgttttggt agcgtgcggg
    10501 atgaagtcaa tgagttggca gaagaactgg gggctgatgt tgtagttatc ggttctcgca
    10561 acccatcgat ttcgacccat ctgttaggtt ctaacgcctc gagcgtgatc cgccacgcca
    10621 atctgcctgt actggtcgtt cgctaacaga taaaagtatt tatgccgccc tgcctggaac
    10681 atcccggcag ggctttttaa tgccataaaa aagccgccag gtttgacccc ggcggctaag
    10741 caattgcagg tgaatcttac ttcttcttat gcagttttgg tgcgaatcag gtagtcaaaa
    10801 gcactcagag aggctttggc accttcgcca gtggcgatga tgatctgctt gtacggaacc
    10861 gtcgtacagt cacccgctgc gaacacgcct ttgacgttgg tttcacattt cgcatcaatg
    10921 ataatctcgc ccatgcggtt acgttcgact gcgccttcca gccagttggt gttcggcagc
    10981 agaccaatct ggacaaaaat accggccagt tcgatgttgt gaatattgcc gctgacacga
    11041 tctcgatatt ccagaccaac gactttgctg ccgtcgcctt tcacttccgt ggtttgcgca
    11101 ttcagaataa tgtcgacgtt tttcaggctg cgcagtttgt cctgcagaac ctggtcggct
    11161 ttcatttctg gcgcaaattc cagcagcgtt acgtgctcaa cgatacccgc caggtcgatt
    11221 gccgcttcca cgccggagtt accgccgccg ataaccgcta cgcgtttgcc tttaaacagc
    11281 gggccgtcgc agtgcgggca gtaggtcacg cctttagtgc gatactgatc ttcgcccgga
    11341 acgttcatgt tgcgccattt tgcacccgtc gccacgataa tgctgcgtgc tttcagtacc
    11401 gcgccagaag ctgtttcaat ctggtgtaga ccaccttcaa ccgctgccgg gatcagtttg
    11461 ctggcgctct ggctgtcgat cacatcaacg tcgtattcat caacgtgaac tttcagtgcg
    11521 cctgccaact tctgcccttc agtcttcggt acagaaatgt agttttcgat atcaacggta
    11581 tcgaggatct gaccaccaaa acgttcgccc atcagaccgg tacggatgcc tttacgtgcg
    11641 gagtaaattg ctgccgctgc acccgccggg ccggaaccaa cgattaatac gtcataagca
    11701 tcacgcttgt tcagctcttc tgccgcacga ttttccgcgc cagtatcaat tttggcaacg
    11761 atttcagtca acgtcatgcg gccctgacca aactctttcc cgtttacgaa cactgccgga
    11821 acgcccatca cgttgcgatc ggtgatttcg ttctggaagg tgccgccgtc aattgcagtg
    11881 tgcttgatgc gcgggttcaa tacgctcatc aggttcagcg cctgcaccac gtccgggcag
    11941 ttatggcaag agagcgagta ataggtttcg aattcaaaat caccgtcaat atggcgaatc
    12001 tgctccagca gagactgcgc ttctttcgac ggatgaccac cggtccacag caacgccagt
    12061 accagcgagg tgaactcgtg gcccagcggg gagcctgcaa aacgtggtcc ctggttggaa
    12121 cctgggttgg tgatcaggaa agacggctta cgcactggca agctgttatc ttctttaaag
    12181 gtgactttgt ctgacagttc tgcgatttca gccaacagtt ccttgatttc tgccgattta
    12241 gcgctgtcat ccagcgtggc aattaactca acaggcttgg tcaatttctc aaggtaagcc
    12301 ttgagttgag ttttcatatt tgtgtcgagc atgtttatct cctgggcttt aaacatcatc
    12361 atgcaagcgg cctctgcggc tacctggatg cagcttgcat catggtgcgg ggaagaaaat
    12421 cgggtgccgc agcacccgaa gaattaggtg aatttccgaa gtatttcgcg tcagagcaag
    12481 gcgactagcc tgtgaatccc cgggagctta cacaagtaag tgaccggggt gagcgggcga
    12541 cgccaacgcc gctatggcgt gaaagacgaa ggaaatttag attttaccaa ccaggtccag
    12601 agacggagcc agagttgctt caccttcttt ccatttagcc gggcaaactt cacctgggtg
    12661 agaagctacg tactgagctg ctttgatttt acgcagcaga tcagacgcgt cacggccaat
    12721 gccttcagcg gtaacttcga ttgcctggat gataccctgc gggtcaacaa cgaaggtcgc
    12781 acggtcagcc agaccttcat cttcacgcat gttgtcgaag ttacgggtca gggcgccagt
    12841 cgggtcgccg atcatcgcat atttgatttt agcgatggtt tcagagctgc tgtgccatgc
    12901 tttgtgggtg aagtgagtat cggtagatac tgcgtatacg tctacgccca gtttctgcag
    12961 ttcttcgtag tggtcagcaa cgtcacccag ttcggtcggg catacgaaag taaagtcagc
    13021 cgggtagaag aagaagacgc tccagcggcc ttcggtatct ttttcggtga tttcgatgaa
    13081 ttcgccgttt ttgaatgcct ggtttttaaa aggtttaatt ttggtgttaa tcaaggacat
    13141 ctatacttcc tccgtgtttt cgatgagatg taaggtaacc aatttttgcc gattcggcta
    13201 atgcgtttcc attatcaaat cgataagttt taccttacaa ccttcgtaag acaactttgt
    13261 gaactttgca ccgccagcgg cgataaaaag taaaatggcc ccctttccag gtgaccgtgt
    13321 aatctgaact accctttggt atcttcagcg cctgcctgca ggctgaggtg ttgcccttac
    13381 aaatgcaaca acgacatgga ttacaacacc ctcataaaca aagggcaatc acctgatcta
    13441 agctcttacc tatgacagtg ataggttatg ccttttactc gacttttgca ctgactgaaa
    13501 agaacaaatt aatgttaaaa aagatacttt tactggctct gcttcctgca atcgccttcg
    13561 cagaggaact tccttcccca gtaaaagcga ttgaaaaaca gggcattaca atcatcaaaa
    13621 cgttcgatgc ccccggagga atgaaaggtt atctcggaaa gtatcaggat atgggcgtca
    13681 ccatctacct gactcccgat ggtaagcacg ctatctctgg ttacatgtac aacgagaaag
    13741 gtgaaaacct gagtaacaca cttatcgaaa aagaaattta cgcaccagcc ggacgcgaaa
    13801 tgtggcaacg gatggaacaa tcccactggc tcctcgacgg taaaaaagat gcgccggtca
    13861 ttgtctacgt cttctccgat ccgttctgcc catattgtaa acagttctgg cagcaggcgc
    13921 gcccgtgggt agattctggc aaagtgcaat taagaacatt gttggttggg gttatcaagc
    13981 cagaaagccc ggcgacagca gcggcaattc ttgcctccaa agatcccgca aaaacctggc
    14041 aacaatatga agcctctggt tgcaagctta agctaaacgt gcctgcaaac gtaagtacag
    14101 agcaaatgaa agtgttaagt gacaatgaga aactgatgga cgatctgggg gcaaatgtca
    14161 cgccggctat ctattacatg agtaaggaaa atacgctgca acaggccgtg gggttgcccg
    14221 atcagaaaac gcttaatatc attatgggaa ataaataaaa ggtaattcat ggatggttga
    14281 actatatccc tgcttttcaa ccatcctaaa aaagcatgaa tttctcgctc taccctaagt
    14341 ggctgtgcta ttttagaaaa acttaatcct gagttaagca aaaaaacata atcaataaaa
    14401 atacata
//
LOCUS       DAZXUK010000089        14346 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZXUK010000089 DAZXUK010000000
VERSION     DAZXUK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14346
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.089"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..3051
                     /locus_tag="ACH3L8_002086"
                     /pseudo
     CDS             1..3051
                     /locus_tag="ACH3L8_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="ACH3L8_002087"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="ACH3L8_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="ACH3L8_002088"
     CDS             6768..7175
                     /locus_tag="ACH3L8_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085205.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="ACH3L8_002089"
     CDS             7717..8805
                     /locus_tag="ACH3L8_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFT4085206.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..11095
                     /locus_tag="ACH3L8_002090"
     CDS             8816..11095
                     /locus_tag="ACH3L8_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFT4085207.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11088..12225
                     /locus_tag="ACH3L8_002091"
                     /pseudo
     CDS             11088..12225
                     /locus_tag="ACH3L8_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            12222..14252
                     /locus_tag="ACH3L8_002092"
     CDS             12222..14252
                     /locus_tag="ACH3L8_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085208.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASVDAFHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVG
                     SGIAGEAKLDHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSP
                     AATRRLFSFQAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPL
                     RQPGIVALREYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEG
                     EDNLLTLSLPASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKT
                     RAWALDAETAPVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQA
                     ELLANDLTAVGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3550 a         3769 c         3662 g         3365 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacctggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatta tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgcggcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggccc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     9061 ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt
     9121 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
     9181 gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt
     9241 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
     9301 gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg
     9361 gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca
     9421 cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt
     9481 gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct
     9541 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg
     9601 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg
     9661 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     9721 actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     9781 aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     9841 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     9901 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact
     9961 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
    10021 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    10081 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    10141 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactcctgga
    10201 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac
    10261 cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga
    10321 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
    10381 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
    10441 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag
    10501 accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct
    10561 gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgcgg cggtagcctt
    10621 gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg
    10681 tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    10741 gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca
    10801 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag
    10861 tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc
    10921 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
    10981 cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca
    11041 actcgaacag caggcactgg catcgctgca acactgggga gttttccatg tctgaactga
    11101 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    11161 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc
    11221 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct
    11281 caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    11341 ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacaaatc
    11401 tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca
    11461 aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg
    11521 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    11581 gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
    11641 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaattc ccccgcttta
    11701 acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg
    11761 gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac
    11821 ccggcattcg tacccatctg gtggcgtttg accacaagcg tcgttgatct cacggcagac
    11881 gttgccgatc cggtagagtt attaatgaaa gtgcaattag gcggcggaac caatatcgcc
    11941 agtgccatgg agtatggtcg gcaacttatt gaacaacctg caaaaagcgt cattatcctc
    12001 gtgagcgatt tttatgaagg gggttcatca tcattgctga cgcaccaagt gaaaaagtgt
    12061 gtccagagcg gcatcaaagt gctgggactg gcagcgctcg atagcacagc aacaccttgc
    12121 tatgaccgcg atacggccca ggcgctggtt aatgtcggcg cacaaatagc cgccatgaca
    12181 ccgggcgagc tggcatcatg gcttgcggag aatcttcagt catgaattca ctacgtccgg
    12241 aattattaga actgacaccg caggcactga cggcgttaag caatgccggt tttgttaagc
    12301 gcagccttaa ggaactggaa aatggcaacg tcccggaaat cagccatgag aacagcgctt
    12361 taatcgccac cttcagtgac ggtgtccgta cccagctggc gaacggccag gcactgaaag
    12421 aggctcagtg cacctatggg gccagcggca tgtgccgtca tcgcgtgatg ctggtgttaa
    12481 gttatcaacg actttgtgct accgcccagc ctacgggaaa agaagaggaa tgggatccgg
    12541 caatctggct ggaagaactg gctacccttc ccgatgccac ccgcaaacgc gcacaggcgc
    12601 tggtcgctaa aagcatcacc attgagttgt tctgtgcgcc gggtgaaatt ccctctgccc
    12661 gcttaccgat gagcgatgtg cgtttttatt cccgcagcag tattcgtttc gcccgttgtg
    12721 attgtattga aggcacactt tgcgaacatg tcgtactggc agtacaggcc tttgtccagg
    12781 caaaagcgca gcaagcggaa ttgactcatt taatctggca gatgcgcagc gaacacgtca
    12841 catcatctga cgatccgttt gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcaac
    12901 aactaagcca ggcattatgg ctgagcggca tcagccagcc gcttattcac tacgaggccg
    12961 ctttcagccg cgcgcagcaa gcagcggaac gctgcagctg gcgatgggtg agtgaatcgc
    13021 tacggcaact gcgcgccagc gttgatgcct tccacgcccg cgccagcgtt gatgccttcc
    13081 acgcccgcgc cagccactat catgccggag aatgcttacg tcagcttgcg gcattaaaca
    13141 gtcgattaaa ttgcgcacaa gagatggccc ggagcgacag tgttggtgaa gttcctcctg
    13201 tgccgtggcg cacggtcgtt ggctctggca ttgccggaga agcaaagctt gatcatctgc
    13261 ggctggtgtc tttaggtatg cgttgctggc aggatattga gcattatggt ttacgcatct
    13321 ggtttaccga tcccgacacc ggcagtattt tgcacctttc gcgcagttgg ccgcgaagtg
    13381 aacaggaaga ctcaccggca gctacgcgtc ggctgtttag ttttcaggct ggcgcactgg
    13441 cgggtgggca aattgtttca caagcagcaa aacgcagtgc cgatggcgag ctgctgttag
    13501 ctacccgcaa ccgcttaagc agcgttgtac cgctgtcgcc tgatgcctgg caaatgttga
    13561 gcgcgccgtt acgccagccg ggcattgtgg ctttgcggga atatttacgc cagcgtcccc
    13621 ccgcctgcat acggcctctt aatcaggtcg ataacttatt tattctgccg gtcgctgagt
    13681 gtatttcgct cggttgggat agcagccgcc agacgctgga tgcgcaggta atcagcggcg
    13741 aaggtgaaga taatctgctg acgttatcac tgccagcctc agccagcgcg ccttatgcca
    13801 ttgaacgcat ggcggcgctt ttgcaacaaa cagacgaccc cgtgtgtctg gtttctggct
    13861 ttgtcagttt tgttgatggg caattgacac tggaaccaca ggtaatgatg acaaagactc
    13921 gtgcctgggc gctggacgca gaaactgcgc ctgtggtggt atcactacct tctgccagcg
    13981 ttctgcctgt gccgtctact gctcatcagt tgctgatgcg ctgccaggcg ttacttattc
    14041 aactgctcca taacggctgg cgctatcagg aacagagcgc tattaatcag gcagagttgc
    14101 tggcgaatga cctcaccgcg gtgggttttt atcggctggc acatgtgttg gcacaatttc
    14161 gtaatacaga aagcgaggca cgggtagaag caatgaataa cggtgttttg ctttgcgaac
    14221 aattattccc catgcttcag caacaaggat gaaatagtgc tttttactaa gagttctact
    14281 ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc ctggtgagag
    14341 tcacca
//
LOCUS       DAZXUK010000090        14345 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZXUK010000090 DAZXUK010000000
VERSION     DAZXUK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14345
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.090"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..185
                     /locus_tag="ACH3L8_002093"
     CDS             <1..185
                     /locus_tag="ACH3L8_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFT4085209.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="ACH3L8_002094"
     CDS             complement(210..401)
                     /locus_tag="ACH3L8_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFT4085210.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="ACH3L8_002095"
     CDS             complement(506..742)
                     /locus_tag="ACH3L8_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFT4085211.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="ACH3L8_002096"
                     /pseudo
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="ACH3L8_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="ACH3L8_002097"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="ACH3L8_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="ACH3L8_002098"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="ACH3L8_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFT4085212.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYRRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="ACH3L8_002099"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="ACH3L8_002099"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFT4085213.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="ACH3L8_002100"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="ACH3L8_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFT4085214.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="ACH3L8_002101"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="ACH3L8_002101"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFT4085215.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="ACH3L8_002102"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="ACH3L8_002102"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFT4085216.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="ACH3L8_002103"
     CDS             complement(10860..12233)
                     /locus_tag="ACH3L8_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4085217.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="ACH3L8_002104"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="ACH3L8_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFT4085218.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="ACH3L8_002105"
     CDS             complement(13344..13583)
                     /locus_tag="ACH3L8_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFT4085219.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="ACH3L8_002106"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="ACH3L8_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFT4085220.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="ACH3L8_002107"
     CDS             13960..14247
                     /locus_tag="ACH3L8_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFT4085221.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3762 a         3512 c         3684 g         3387 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggttgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaactgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcca ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctacgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtat
     1561 aggtcgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtctct gcactcaaac tcatggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagtatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgtcggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac tgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgacgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgcagat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagagg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actct
//
LOCUS       DAZXUK010000091        14294 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZXUK010000091 DAZXUK010000000
VERSION     DAZXUK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14294
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.091"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..79
                     /locus_tag="ACH3L8_002108"
     CDS             <1..79
                     /locus_tag="ACH3L8_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085222.1"
                     /translation="RAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACH3L8_002109"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACH3L8_002109"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFT4085223.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="ACH3L8_002110"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="ACH3L8_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFT4085224.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACH3L8_002111"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACH3L8_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFT4085225.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACH3L8_002112"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACH3L8_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFT4085226.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACH3L8_002113"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACH3L8_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFT4085227.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="ACH3L8_002114"
     CDS             5377..5835
                     /locus_tag="ACH3L8_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFT4085228.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="ACH3L8_002115"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="ACH3L8_002115"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFT4085229.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="ACH3L8_002116"
     tRNA            6887..6971
                     /locus_tag="ACH3L8_002116"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="ACH3L8_002117"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="ACH3L8_002117"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACH3L8_002118"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACH3L8_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFT4085230.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACH3L8_002119"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACH3L8_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFT4085231.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACH3L8_002120"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACH3L8_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFT4085232.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="ACH3L8_002121"
     CDS             complement(10252..13995)
                     /locus_tag="ACH3L8_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFT4085233.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3541 a         3764 c         3423 g         3566 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcg
//
LOCUS       DAZXUK010000092        14147 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZXUK010000092 DAZXUK010000000
VERSION     DAZXUK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14147
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.092"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            44..676
                     /gene="comR"
                     /locus_tag="ACH3L8_002122"
     CDS             44..676
                     /gene="comR"
                     /locus_tag="ACH3L8_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFT4085234.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(738..1277)
                     /locus_tag="ACH3L8_002123"
     CDS             complement(738..1277)
                     /locus_tag="ACH3L8_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFT4085235.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ACH3L8_002124"
     CDS             complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ACH3L8_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFT4085236.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3216..3758)
                     /gene="ycfP"
                     /locus_tag="ACH3L8_002125"
     CDS             complement(3216..3758)
                     /gene="ycfP"
                     /locus_tag="ACH3L8_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFT4085237.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3781..4806)
                     /gene="nagZ"
                     /locus_tag="ACH3L8_002126"
     CDS             complement(3781..4806)
                     /gene="nagZ"
                     /locus_tag="ACH3L8_002126"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFT4085238.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(4817..5639)
                     /gene="thiK"
                     /locus_tag="ACH3L8_002127"
                     /pseudo
     CDS             complement(4817..5639)
                     /gene="thiK"
                     /locus_tag="ACH3L8_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
     gene            complement(5620..6261)
                     /gene="lpoB"
                     /locus_tag="ACH3L8_002128"
     CDS             complement(5620..6261)
                     /gene="lpoB"
                     /locus_tag="ACH3L8_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFT4085239.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6276..6653)
                     /locus_tag="ACH3L8_002129"
     CDS             complement(6276..6653)
                     /locus_tag="ACH3L8_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFT4085240.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6656..7015)
                     /gene="hinT"
                     /locus_tag="ACH3L8_002130"
     CDS             complement(6656..7015)
                     /gene="hinT"
                     /locus_tag="ACH3L8_002130"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFT4085241.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7354..9539
                     /gene="fhuE"
                     /locus_tag="ACH3L8_002131"
                     /pseudo
     CDS             7354..9539
                     /gene="fhuE"
                     /locus_tag="ACH3L8_002131"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(9599..11032)
                     /gene="ptsG"
                     /locus_tag="ACH3L8_002132"
     CDS             complement(9599..11032)
                     /gene="ptsG"
                     /locus_tag="ACH3L8_002132"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFT4085242.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11327..12124)
                     /locus_tag="ACH3L8_002133"
     CDS             complement(11327..12124)
                     /locus_tag="ACH3L8_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFT4085243.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12135..13139)
                     /gene="holB"
                     /locus_tag="ACH3L8_002134"
     CDS             complement(12135..13139)
                     /gene="holB"
                     /locus_tag="ACH3L8_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFT4085244.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13136..13777)
                     /gene="tmk"
                     /locus_tag="ACH3L8_002135"
     CDS             complement(13136..13777)
                     /gene="tmk"
                     /locus_tag="ACH3L8_002135"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFT4085245.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13767..>14147)
                     /gene="mltG"
                     /locus_tag="ACH3L8_002136"
     CDS             complement(13767..>14147)
                     /gene="mltG"
                     /locus_tag="ACH3L8_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFT4085246.1"
                     /translation="IEKETAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNG
                     KLSRADLETPTAYNTYTITGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTF
                     NTNLASHNKSVQDYLKVLKEKNAQ"
BASE COUNT         3549 a         3760 c         3456 g         3382 t
ORIGIN      
        1 ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa ctgactcaac
       61 acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga
      121 taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt
      181 cgaagcaacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt
      241 atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct
      301 gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg ccatcgccaa
      361 ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact gcaccaccct
      421 ctccccagat tcaggagata tagccaatac gttgaaatca cgccatgcga tgcaagagcg
      481 cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga
      541 cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg
      601 cgaaggtgca tcgctggaaa aactgatgca gattgccgga acgactttgc gtttatggcc
      661 cgaactggtg aaataagtcg caaaaaaggc ccctcagcct gagcgaatga ggggccaaat
      721 tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccgtt actatctagc
      781 gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta
      841 taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt
      901 tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat
      961 ccaccaccca gcgcccccac aacagtggcg acatctttac cgcgaccacc accaaactga
     1021 tgccctatca cgccaccagc aacagcgccg agcaccgacc cggtaatgcg attttcatcc
     1081 tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc
     1141 gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccccg ttcaaacacg
     1201 ttcagactgg ccactgccgc tacgcccaac gcagctgcga caccaatccc gatacccgcc
     1261 aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc atgcaagcga
     1321 aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatgactgg aaatacttaa
     1381 ggattcggag gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta
     1441 ccgctcgtac catttgtcca gggcggataa ggcgtttata ccacatccgc cagtgtacgt
     1501 cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat
     1561 taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata gcgagatata
     1621 tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag
     1681 gctaccaacg gtggagaagt tcgacagcga taccagcgaa ccatgatctt tatactgata
     1741 atttttcagc ggcttaccgt tcatctgcgc cagaatgttg ttcattgcgc aagtcgccat
     1801 ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg ggcatgacgc
     1861 gcagtcgcca atagcgtaaa tgtctggatc gcgagtggtt tgcagcgtcg gttccaccac
     1921 cagctggttg atacggttag tttcaagacc accgatatct ttcaggaagt ctggcgcttt
     1981 gatcccggct gcccatacca tcagatcagc ctcaatatat tcgccatctt tagtgtgcag
     2041 gccgccttca tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt
     2101 tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttctcc
     2161 cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg
     2221 cagttgcttg atcgcgttgt gcaattcagc ggagagttct acacccgtcg cgccgccgcc
     2281 gacaatcgca atgttcactt tgccatttgc gcccaggttg gcagagtatt tcaggaacag
     2341 attcagcatc tcctggtgga agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt
     2401 ctctttgacg cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca tgaccagggt
     2461 gtcataggcg attttacgtt ccggaaccaa cagttcacct ttctcgtcgc gtagttctgc
     2521 aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccaact ggaactggaa
     2581 accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc
     2641 agtcgccact tcgtgcagca gtggtttcca caggtggctg tggttacgat cgaccagcgt
     2701 aattttggct tttttcttac gtcccagctt atgccccagc tgtgttgcca tttccagccc
     2761 accagcaccg ccgccgacaa tcacaatctt tttcaatggc gtagtcaacg tgcccccctt
     2821 aaatttatta accaattgtt aattaatggt tattaacata gctcttaatt aacaacaggt
     2881 tacagcaatg aaaatgttct tcgctattga gaataacatg aatggtgcat tggtcatacc
     2941 aaaattgatg ttaatcaagt tttgttgctt aaaagatagg caactttacc gggggagatc
     3001 aaaaaataga gtttattgtt tattatttga gcaaattcgg aagggtaata tagggtgaag
     3061 cgtatatcga gggcgtttgg tcgtcctgat aagatgcgca gcatcgcatc aggcattgat
     3121 gctcgactgt cggatgcggc gtggacgcct tatccgactt acagaattcg gagcgcatca
     3181 ggtaccggca aggttacctg atgcgacgag ggcatttacc cgagggtttt gaacgcttta
     3241 atgcgctgta aatgcgggga gatattcttg aatttgtgcg tctgctcttc gtcccagaca
     3301 atctcgtaat aatgatgcaa ctcttcagat gtccgctggc tgttaagtgc ttcatcatta
     3361 cgcgacaaaa taaccagaca acgatcgcga ttcttctcgc ggaagttggt cacacactta
     3421 gtggcaatat cggcatactc ttccgggcga tcaatcttgc cttccatgtt ctcataaggg
     3481 aacaaattag ggttgaagat cacctggcgg atatcgcaaa gaaaaccaat ccgttccgcc
     3541 cagtatccgc ccaggccaac gccgcaaatt agcggacgct cgtcaacgtt cagttgcaac
     3601 attttgtcca cttctttaag cagatgctgc atatcatgtt tcggatgccg cgtactgtag
     3661 cttatcaagc gtacatccgg gtcaataaac tgcaattgta agactttctc gtggttaccc
     3721 ggactgttag agtcaaaacc gtgtaaatag ataatcatcg cttcctcaca taagccaggg
     3781 ttagtgacct actttctctt cctgccagcg ttcatgtaac tgattcagac gggtgctgat
     3841 cgctttccag cgagccgagt ccatcagttc ctgtcgcgaa aatgaacctt tatgatacaa
     3901 acgtgtaaca cgttctgcct tgatcggtga cagattatct aacacgctga cggccccttt
     3961 acgattattg cagaccagga tcatatcgca acccgcatcc agtgatgcct gcccgcgttc
     4021 ggcataactg cccataatcg cggcaccttc catcgataaa tcgtcagaga aaatcacgcc
     4081 gtcaaacccc agttcctgac gcaaaacggt tttcagccag tagggagaac cgctcgccgg
     4141 acgcggatca acatcactgt agatcacatg cgcaggcata atggcgtcga gtttattttc
     4201 gcggattaag gaactgaaga ccgacatatc tttagcgcga atctccgctt gtggacgtgg
     4261 atcgcacggt gtttctttgt gtgagtctgc cgttactgca ccgtgtcctg ggaagtgttt
     4321 cccggtcgtt ttcattccgg cttcatgcat accatcaata aaccggctgg caattgccag
     4381 ggctttttgt ggatcggcat gataagaacg ctcgccaatc gccgcgctga tatgcccgac
     4441 atccagcact ggcgcaaagc tgatatcaat atccatagcg atcatttcgc tggccatcag
     4501 ccaacccgcc tcttgcgcca gtttgccgcc ctcttccatt cccaacagcg cagcaaagga
     4561 ttgtgctgcc ggtaagcggg taaaaccttc gcgaaaacgc tgcacgcgtc caccttcctg
     4621 atctaccgcc accaccagat gattgcgcga tgctgcgcgg atctggcgca ccagttcacg
     4681 taactgggca ggatcatgat agttacgcgt aaagagaatc agccctccca ccagcggatg
     4741 cgccagtatt tcacgctctt ccgcgtccag ttcgtaacct tcgacatcca acattactgg
     4801 acccacactg ctctccttat tgttttatta atagctgccg ccaggtgtca tcggccagcc
     4861 tgataaattg ttgatcgccg gtttgtcgcc agcggtactc aaaccaccct cctttgagca
     4921 tcagcagcca gggaaaccat cgcctgacct gacgccataa ttgcgccgga taaatcttcg
     4981 cgcgagtggc atagtcattg accaattgcc ggtgctgttc agtattttcc acccacaccg
     5041 ccgccagttc cagcgcgata tcaccatctc cagcatactc ccagtcgatg agttttaacc
     5101 ctgatgcgct atgcactaaa tttggcgtgg acatccatat gcaatggact taagcgtaaa
     5161 ggtcgtggtt cccgcgcttt gcgcagacgt tttaacattc gcagccaacc cacagtccgc
     5221 cgcgccggat cgctttgctg ccagtacagt tccagtaagg gcaacagcgt tattcgccag
     5281 ccaaaacgtg gttgttgatg tagataatac agcaagcctg ccagttcgtt ggtatccggc
     5341 aaatacgttt ttaccgcacc gggcagatag tcgactacca tccagtcacg gagatataaa
     5401 tgcggcttcg gtgcaatgct tgcgggtagt tgtgataaag cccgatactg gcgcaagaac
     5461 gcggactgcg gcgcatcagg atcgtgcggc tgacgcacaa caaaacgctg gccctgatgt
     5521 tcaatgagaa aactaccgcc actaagccca ctattaaacg tcgtgacggg atgaaactgc
     5581 gggaaaaagc gcgacagcaa ttcgtcgcgc gtgatgggat tattgctgcg aaacggcacc
     5641 tttacctgac cagataattt cgcccgtctg caccagcatc agctgcattt gtagggtcgg
     5701 agcgttaacg ttgccagagg cgcaggagta cagcacgtaa tgagcgccga cattgcgggc
     5761 aatgcctatg gctttactac gggtgcctaa actgtcctgc ggcgacaaac ctaactgttg
     5821 cttagccatc gacagttgct gggcggaaac cagggtaaat ttcccgttat tagccagcgc
     5881 atttcgcagc gtttcggtcg cttctgcggc attcagcgaa ccgttagtac ggttgttaac
     5941 gctatcaacc agcaggacgc tacccgcagt caccccgtca gccccaagca tcttactgac
     6001 catcggctgc attgcgccat tccagtcata atggcgaata tgcggcgcag gcggtgccgt
     6061 tcgatcttcg tgctcaattg ggcctggctg ctgcgggatc gtcggcaccg agggcactgt
     6121 ggggacaggc tgttgtggct cggctggttg ttccggcgct ggtttcactt cttctaccgg
     6181 tgcaggttca cgttgcccca cacacccggc gagaaacatc gccagcgcgg taatcaaggc
     6241 gtagcgactc atttttgtca tcaagattca cccctttaca aatatagata aagtctgact
     6301 ttgtgcgccc ccagaaaatt ggcgctgccg tacagcgtta ccgccgaatg tgcgggaatg
     6361 gtaacgctgc gtggcctttc cagaggatgc atctccagcc ctctggcgtc ataccagtaa
     6421 aaacgataat gaacggtaac gggttcttgc ctttcgttat aaatcgttga ggatgctgaa
     6481 ggttgaatat cagacgtcga aaggacgggc ttttctgcac tgatgcccgc agccaataaa
     6541 gatgactcca tcaccagcga ttgctcatca ttcaccggaa tttccggatg tgaacgacag
     6601 cccaccagca gtaacaacac cagagacacc agcccaaagc atccttttct catcgttaca
     6661 gacctttatg cgccagcatt ggtcccagcg gacggccacc caacaagtgc atatggatgt
     6721 ggtaaacctc ttgtccgcca tggcggttgg tattcatgat cagacgatag ccatcttcgg
     6781 caataccttc ttgctcagca atttttgccg ctacggtgat catgcgtccc agcgcctgct
     6841 catgctcagc tgacacgtcg ttcacagtcg gaatgagaat attcggaatg atcagaatat
     6901 gcgttggcgc ttgcggcgaa atatcgcgaa acgccgttac cagatcatcc tggtagacga
     6961 tatcggaggg gatctcacga cgaataattt tgctgaatat agtttcttct gccacgacat
     7021 tttccttttt caaatagcca tgcgttgcgc catgctacgc cgcgccacac tgcgagtata
     7081 gagtatggtc gagttaaacc tgctctttca atcttaactc acgatttctt aagcaaaaaa
     7141 tcgttttgct gccgtatata tcgccattat tcccatttct gcctgcgaat gcgctgaaaa
     7201 gacattctct gtttggttta cacgacaaat atgaatgcgt atatttctca tttgcatttg
     7261 catttgcagc aaaattattc gcacactaaa tataaccgct catcaccctc ctccggcatg
     7321 agcctgacga caacataaac caagagattt caaatgcttt caacacaatt taacagggat
     7381 aatcaatatc aagtcatcac caaaccgtca ctacttgccg gttgcatagc actggcacta
     7441 ttaccttctg ccgcttttgc tgcaccagcc actgaagaaa cggtgattgt tgagggttca
     7501 gccacagctc cagatgatgg cgaaaatgat tacagcgtaa cgtctacctc tgcgggtacc
     7561 aaaatgcaga tgactcaaca tgatattcct cagtcggtca ctattgttag ccagcagcgg
     7621 atggaagatc agcagttaca aacgctgggc gaagtgatgg aaaacacgct ggggatcagc
     7681 aaaagtcagg cggattccga tcgtgctctt tattattccc gcggattcca gatcgataac
     7741 tatatggttg atggtatccc cacctatttt gaatcgcgct ggaatctggg cgacgcactt
     7801 tctgatatgg cactgtttga acgcgtagaa gtagtgcgtg gcgcgacagg actcatgacc
     7861 gggacgggta atccatctgc ggcaattaat atggttcgaa aacacgcgac ctgtcgtgaa
     7921 tttaaaggcg atgtctcggc ggaatacggt agctggaaca aagaacggta tgtggcggat
     7981 ttacaaagcc cactcaccga agacggtaaa atccgcgcgc gaattgtcgg cggctaccag
     8041 aataacgact catggctgga ccgctacaat agtgaaaaga ccttcttctc gggcattgtc
     8101 gatgctgatt taggcgatct tacgacgctt tcagccggtt acgaatatca gcgcattgat
     8161 gttaatagcc caacctgggg cggtttaccg cgctggaata ctgatggcag cagcaacagt
     8221 tacgatcgcg cacgcagtac cgcacctgac tgggcgtaca acgataaaga gatcaacaag
     8281 gtctttatga ccctgaagca gcggtttgct gatacctggc aagcgacact gaatgccacc
     8341 cactctgaag tcgaatttga cagcaaaatg atgtatgtcg atgcctatgt aaacaaagcg
     8401 gatggtatgc tggttgggcc atacagtaat tatggacctg gctttgatca tgtcggcggc
     8461 accggttgga acagtggcaa acgtaaagtt gatgcgctgg atttgttcgc tgacggtagt
     8521 tatgaattgt ttggtcgtca gcacaatcta atgtttggtg gcagttacag caaacaaaac
     8581 aatcgttcag ttcatgggcc aacatcttcc cggatgaaat tggcagtttc tacaacttta
     8641 atggcaattt cccacaagcc gactggtcat cacagagcct ggcgcaggac gataccacac
     8701 atatgaaatc gttatatgct gccactcgtg tcacccttgc cgatccgctg catctgatcc
     8761 tcggcgcacg ttataccaac tggcgggttg atacgctgac ttacagcatg gagaaaaacc
     8821 acaccacgcc ttacgctggt ctggtgtttg acatcaatga caactggtcg acctacgcca
     8881 gctatacctc tattttccag ccgcaaaatg atcgtgacag ttcaggcaaa tatctggctc
     8941 caatcaccgg taacaactac gagctgggtc tgaaatcgga ctggatgaat agccgtctga
     9001 ccaccacgtt agccatcttc cgtattgagc aggataatgt cgctcagtcc accggtacac
     9061 ctatccccgg cagcaacggc gaaaccgcct ataaagcggt ggatgggaca gtcagtaaag
     9121 gggtggaatt tgaactcaac ggcgcaatta ccgacaactg gcagctgaca tttggcgcaa
     9181 cgcgctatct tgcagaggat aacgaaggaa acgccgttaa tcctaatctg ccacgcacca
     9241 cggttaaaat gttcaccagc tatcggttgc ctgtcatgcc agagttgaca gtcggcggtg
     9301 gtgttaactg gcaaaatcgc gtgtataccg acaccgtgac accgtatggc accttccgcg
     9361 ccgagcaagg tagctacgcg ctggtggatc tcttcacccg ctaccaggtg acgaaaaact
     9421 tctcgttaca ggggaacgtc aataacctgt tcgacaaaac ctacgatacc aacatggaag
     9481 gttctatcgt ctacggcgca ccgcgtaatt tcagcattac cggcacgtat caattctgaa
     9541 taacacctgt aaaaaaggca gccatctggc tgccttagtc tccccaacgt cttacggatt
     9601 agtggttacg gatgtactca tccatctcgg ttttcaggtt atcggattta gtaccgaaaa
     9661 tagcctgaac accagaacca gcaaccacta cgcccgctgc gcccagtttc ttcaggccag
     9721 cctgatccac tttagacaca tcagcaacgc tgacgcgcag acgggtaata catgcgtcga
     9781 ggttagtaat gttttcttta ccaccaaatg cagcaaccag agccggtgcc atttcgctgg
     9841 tacctgttgc ttttgcatct tcagtcgcgt cttcacgacc cggcgttttc agatccagtg
     9901 ctttaatcag cacgcggaag atggtgtagt aaacaatcgc ataaccgata ccgacgatcg
     9961 gtaacagcca cagtttgctg ctgttaccag acagaacgat gaagtcgatc agaccgtgcg
    10021 agaacgacgt accgtcacgc atccccagaa gaatacagat tgggaatgcc aggcctgcca
    10081 gaatcgcgtg gatgatgtac aggatcggcg caacgaacat gaagaagaac tcgatcggct
    10141 cggtgatacc ggtcaggaac gaggtcagcg ccgcggagat cataataccg cccactttcg
    10201 cgcggttttc tggtttagca gagtgccaga tagcaattgc ggcagctggc agaccgtaca
    10261 ttttgaacag gaagccacca gacagtttac ccgcagtcgg gtcacccgcc atataacgcg
    10321 gaatgtcgcc gtggaaaacc tgacctgctg cgttggtgta ttcaccaatc tgcatctgga
    10381 aaggtacgtt ccagatgtgg tgcagaccaa acggtaccag gcaacgttcg atgaaaccgt
    10441 aaatgccaaa cgcaactacc gggttctggt aagcagccca ctgagagaag gtctggattg
    10501 cagaaccaat cggcggccaa atgaaggaca gcacaacgcc agtaaagatg gcagccaggc
    10561 cagaaatgat cggcacaaag cgtttaccgg caaagaagcc aagatactca ggcagcttaa
    10621 tacggtagaa acggttaaac atgtacgctg caatcgcacc ggagataatc cctccgagta
    10681 cgccagtatc cgccaagtgt ttagaggcga tttcttcagc aggtaaatgc agtaccagtg
    10741 gcgcaaccac ggccatggtt ttaaccatga tgccataggc aacaactgcg gccagcgcgg
    10801 atacgccatc gttattggta aagccgaggg cgacaccgat cgcaaaaatc agtggcatgt
    10861 ttgcaaagac ggaaccgcct gcttctgcca taacatgcga tacaacggcg ggcagccagc
    10921 tgaaattcgc ggaaccgacg cccagcagaa tacctgcgat aggtagtacg gataccggca
    10981 gcatcagcga tttaccgacc ttttgcaggt tagcaaatgc attcttaaac ataattgaga
    11041 gtgctcctga gtatgggtgc tttttttacg ttctcacgcg tggcaagggg ggagagcctc
    11101 gccgtgtaca gggcatctaa gcgcccttta tttattacac agagtaaaat aattcagtgc
    11161 caatttgttt gacggctatc acgtttcaac ttcagatcat tggagaagtt gcacattttg
    11221 acaaaacata tgtcacaata tttcaaacgc ggttattcac cgcccaattt tcggcggtga
    11281 actttactcg ttttagccat taattacgag ctttaaaaaa actcattcaa cggatggatt
    11341 gaaggcggga agcgtcgatg tgaaacagac gggcgaagtt atcggtggtt acctgcgcca
    11401 gttcttcaac ggcaacacct ttcaacacag ccatgtattc tgcaacgtca cgaaccatcg
    11461 caggttgatt ctcttttcct cgatgcggta ccggcgcaag gtaaggtgag tcagtttcca
    11521 ccagtaatcg atccaggggg acataacgcg cagcatcgcg cagttgctcc gcattacgga
    11581 aggtcacaat gccggaaaag gagatgtaaa atccgagatc cagtaattta cccgccgttt
    11641 ctctgtcctc tgtaaaacag tgtagtacgc caccgcaatc cgtcactttt tcttcgcgca
    11701 gaattgccag cgtatcggca cgggcgtcac gggtatggac gataaccggc ttgttcagtt
    11761 cacgaccaat ctggatatga tggatgaagg actcttgctg acgtacttta gtttccggtg
    11821 tgtaataata atccagcccg gtttcaccca gcgctacaac accctcttct gccgccagac
    11881 ggcgtaaatc ttctacatcg tagggatcat tctggttgag tggatgcacg ccacaagaaa
    11941 atacgacgtt gtcacgttcg cctaccagat cccgcatatg taaataaccc ggtaatgttg
    12001 tggcgactgc cagacaaaat ttcacatcgc gtgcggcggc tttcgccaga acgtcatcca
    12061 cgtccttatg caaagattca taatccagac catcgagatg gcagtgtgag tcgactaaaa
    12121 acatgatgtc tctcttaaag atgaggaacc ggtaacacaa cgcccggttg caggtaatgc
    12181 tcaatacgca gtaaaagatc ggtgatgaga agctcgcggt tgatgcctgt gacagacatt
    12241 aactgttcac gaatgtggca aacatccccc agtatagcct gcaggcacga gggagaaaga
    12301 tggtttgcca gttcggcgac caggcccggc acatcaacat tggtcacctg cgcagcgcca
    12361 tgatggcgtt ttagcgcatc catcagcaac gttgccagcc agtgtaaacg cgccggagct
    12421 tgttcatgat taagggccgc cagcagcgaa taccagtcgc ccgatggcac gctatatgcc
    12481 aacgcctgac acaatgtttc acgagcctgc cagttatctc cctgaaacaa cgccagtgcc
    12541 gcgccaggcg aaccggcgct taagcgcaat gcggcaagta atgcatcctg tgacattgtc
    12601 acttcgcgtg aaagccaagt cacggcgtac tgttctggag gcggcgcaag gtaatgtaac
    12661 cgacaacgac tacgtaatgt tgccagtaaa cgttcaggct cgcgggtagc caggaaaaac
    12721 caggtttctg ctggtggctc ttcaagcgtt ttcagcaatg cgttagccgc ggcgtcggtc
    12781 agtaaggcag catcggttac ccagacaact ttcgcaccac ctaggcgtgc gtgctcattc
    12841 agcttttcgg tgacctcacg taccgcatca atgcccagcg tatttttccc tttttcggga
    12901 gccagggtgt agtaatcggg atgcgtgcca gcctgcatca actgacatcc acgacagtga
    12961 ccgcaacttt tgtggccctg cggttgttgg cagagtaaat aacggctcag ggcgtagatt
    13021 aaagcatcat cgcccatgcc cggtaacgcc tgaatgagta gcgcatggtg acctcttccg
    13081 gcctgatagc tggctaccag tttttcgaaa tcaggtcgta accatggata ccatctcatg
    13141 cgtccaactc cttcacccag tgggtcacgg tagtgcggat tgcatccatc acggcctcca
    13201 gcggctgggt ggcatcaatg gtatgaatac ttttatcttg tgcagccagt tccagatagc
    13261 gggcgcgggt gcgattaaag aaatcgaaag actcttgctc aatacgatcc agctcgccgc
    13321 gcgcacgcgc gcgttttaag ccaacttccg gggtaacatc gagatagagc gttaagtctg
    13381 ggcgaaaatc cccgagaaca gcatcacgca gtgttgccag catatgttgg tcaataccac
    13441 gtcctccgcc ctgatacgcc tgagtggaaa gatcgtggcg atcgccaatc acccaggtgc
    13501 cgttagccag cgcaggtttg atgaccgttt ctaccagttg aacgcgcgcg gcataaaaca
    13561 tcagaacttc ggctttatcg gtaatgactt catcgcctac cgatttgata tccagcacca
    13621 ggcttcttaa cttttcggca agttgcgtac cgccaggttc ccgagtgaaa accatgtcgc
    13681 ggatacccag ttgctcgagc gtctcaacca ccacattacg cgcggtagtt ttgcctgcgc
    13741 cttccagccc ctcaatgacg atatacttac tgcgcatttt tttccttaag cactttcaga
    13801 taatcctgca cagacttgtt atgactggca agattggtat taaacgtgtg accaccttta
    13861 ccatcggcca caaaatagag atacggcgtt tttgccggat gcgcagcagc cttcagcgaa
    13921 tccgcccccg gcgtagctat cgcacccggc ggcaaaccgg taatggtata ggtgttatac
    13981 gctgtcggcg tttccaggtc tgcacgagaa agtttgccat tataacgctc tcccatcccg
    14041 taaatcacgg ttgggtcggt ctgcaggcgc ataccaatgc gtaaacggtt gataaatacc
    14101 gaggcaacct gatcgcgttc actggcaacg gcggtttctt tttcgat
//
LOCUS       DAZXUK010000093        14020 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZXUK010000093 DAZXUK010000000
VERSION     DAZXUK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14020
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.093"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(47..139)
                     /locus_tag="ACH3L8_002137"
     tRNA            complement(47..139)
                     /locus_tag="ACH3L8_002137"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:Ser,seq:gct)
     gene            complement(455..640)
                     /gene="csrA"
                     /locus_tag="ACH3L8_002138"
     CDS             complement(455..640)
                     /gene="csrA"
                     /locus_tag="ACH3L8_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFT4085247.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(875..3505)
                     /gene="alaS"
                     /locus_tag="ACH3L8_002139"
     CDS             complement(875..3505)
                     /gene="alaS"
                     /locus_tag="ACH3L8_002139"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFT4085248.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3633..4133)
                     /gene="recX"
                     /locus_tag="ACH3L8_002140"
     CDS             complement(3633..4133)
                     /gene="recX"
                     /locus_tag="ACH3L8_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFT4085249.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4376..5437)
                     /gene="recA"
                     /locus_tag="ACH3L8_002141"
     CDS             complement(4376..5437)
                     /gene="recA"
                     /locus_tag="ACH3L8_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFT4085250.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5517..6014)
                     /gene="pncC"
                     /locus_tag="ACH3L8_002142"
     CDS             complement(5517..6014)
                     /gene="pncC"
                     /locus_tag="ACH3L8_002142"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFT4085251.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6159..7244)
                     /gene="mltB"
                     /locus_tag="ACH3L8_002143"
     CDS             complement(6159..7244)
                     /gene="mltB"
                     /locus_tag="ACH3L8_002143"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFT4085252.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7500..8062
                     /gene="srlA"
                     /locus_tag="ACH3L8_002144"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7500..8062
                     /gene="srlA"
                     /locus_tag="ACH3L8_002144"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8059..9018
                     /gene="srlE"
                     /locus_tag="ACH3L8_002145"
                     /gene_synonym="gutE"
     CDS             8059..9018
                     /gene="srlE"
                     /locus_tag="ACH3L8_002145"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFT4085253.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9029..9400
                     /gene="srlB"
                     /locus_tag="ACH3L8_002146"
                     /gene_synonym="gutB"
     CDS             9029..9400
                     /gene="srlB"
                     /locus_tag="ACH3L8_002146"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFT4085254.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9404..10183
                     /gene="srlD"
                     /locus_tag="ACH3L8_002147"
     CDS             9404..10183
                     /gene="srlD"
                     /locus_tag="ACH3L8_002147"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFT4085255.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10288..10647
                     /gene="gutM"
                     /locus_tag="ACH3L8_002148"
     CDS             10288..10647
                     /gene="gutM"
                     /locus_tag="ACH3L8_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFT4085256.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10714..11487
                     /gene="srlR"
                     /locus_tag="ACH3L8_002149"
                     /gene_synonym="gutR"
     CDS             10714..11487
                     /gene="srlR"
                     /locus_tag="ACH3L8_002149"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFT4085257.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11480..12445
                     /gene="gutQ"
                     /locus_tag="ACH3L8_002150"
     CDS             11480..12445
                     /gene="gutQ"
                     /locus_tag="ACH3L8_002150"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFT4085258.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12442..13956)
                     /gene="norR"
                     /locus_tag="ACH3L8_002151"
     CDS             complement(12442..13956)
                     /gene="norR"
                     /locus_tag="ACH3L8_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFT4085259.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
BASE COUNT         3261 a         3882 c         3505 g         3372 t
ORIGIN      
        1 tcggttcgta gccgagtact ctatccagct gagctacgga tgcaaatggc ggtgaggcgg
       61 ggattcgaac cccggatgca gcttttgacc gcatactccc ttagcagggg agcgccttca
      121 gcctctcggc cacctcacca cacgcctctt acgagtgctt cgaagaactt gtttatcgct
      181 catcatcgct gcgtggcgca catattactt tctgagactt ataagtcaaa caatttttcc
      241 cacactttta tcgtttgcac acttcacgct caattagtct gtaaaagcgt caaaaagagt
      301 gttttatcta cagaaaaacg gaggtctggc agatagcagt aatgcaaaaa aatggagact
      361 taagttgaat gaacgggagt aaagcgaaaa gactatagag tgaaggagaa attttgaggg
      421 tgcgtctcac cgataaagat gagacgcgga aagattagta actggactgc tgggattttt
      481 cagcctggat acgctggtag atctcttcac ggtgaacaga aacttccttc ggggcattta
      541 cgccaatacg tacctggttg ccctttaccc ctaaaactgt cacggtgacc tcatccccaa
      601 tcatgagggt ctcaccaact cgacgagtca gaatcagcat tctttgctcc ttgaaagatt
      661 aaaagagtcg ggtctctctg tatcccggca ttatccatca tataacgcca aaaagtaagc
      721 aatgacaaac acattacatg taagcagtca tggcattaca ttctgttaaa cctaagttta
      781 gccgatatac acaacttcaa cctgacttta tcgttgtcga tagcgttaat gcgaatgccg
      841 tgaagcgagt ccacggcatt gcctgacgct tatattattg caatttcgcg ctgacccagc
      901 ctttcacact ggctaacgct gcaggtaagg ccgcagcatc cgtaccaccg gcttgcgcca
      961 tgtcaggacg tccaccaccc ttgccgccca cctgctgagc gaccatacca atcagttccc
     1021 ctgctttcac acgatctgtg acgtccttag atacgcctgc aatcagagaa accttacctt
     1081 cggctaccgt tgccagcacg ataattgtcg accccagctg attttttaaa tcgtcaacca
     1141 tggtacgcaa cattttcggc tcaacaccgc taagctcgct aaccaacagc ttaacaccat
     1201 taacatcaat tgccttactg gaaagatttg cgctctcctg tgcggcagct tgttctttaa
     1261 gctgttgtag ttctttttct aactgacgcg tacgttccag tactgagcgc actttatccg
     1321 ccagattatt gctatcgcct ttcagcagat gcgcgacttc gcttaagcga tcgctgtctg
     1381 catgaacggt ggtgatagcg ccttctccgg ttaccgcttc gatacgacga acgcctgcag
     1441 cagtacccga ttcagagatg atgcggaaca gaccaatatc accagtgcgg ctggcgtgag
     1501 taccgccaca cagctcggtg gagaaatcgc ccatgctcag cacgcgtaca cgctcatcat
     1561 acttctcgcc gaacagcgcc atcgcacctt tcgctttcgc cgcttcgaga ttcatgatgt
     1621 tggtttcgat cggcaaattg cgacgaatct gtgcgttcac caggtcttcg accgcacgaa
     1681 tctcttccgg tttcatcgct tcgttgtgtg agaagtcgaa gcgcagcacc ttgtcgttaa
     1741 ccagtgaacc tttctgcgat acatgagtcc ccagaacctg gcgcagcgca gcgtgcatca
     1801 ggtgcgttgc ggagtgattc agacgaatac gggcgcgacg agcctcatca atatcagcct
     1861 gcacagcgtc gcccactttc agagaacccg cagcaagttt accgatgtga ccaatcgcct
     1921 ggccgtattt ctgcgtatct tccaccgcga aggagaagtt agcgcctttc agttcgcctt
     1981 tatcaccaac ctgaccgccg gattccgcat agaatggcgt ttgatccagc acgaccacag
     2041 cttcctggcc ggcattgatg gcatcaaccg ctttaccatc aacaaacagc gcggtcactt
     2101 tgccgttcag ttccagatgg tcatagcctt taaattcaga tgcactatca acacggatca
     2161 ttgcgttgta atcggcacca aagccgctgg cttcgcgcgc gcgacgacgc tgctcttcca
     2221 ttgctgcttc aaaaccagct tcgtcaactt tgatgttgcg ctcacgacaa acatcagccg
     2281 tcaggtcaac cgggaagcca taggtgtcgt acagacggaa agccgtttca ccatccagcg
     2341 tatcaccaga aagttttgcc agctcttcat ccagcaacgc cagaccgcgc tccagagtac
     2401 gggcaaactg ctcttcttca gtcttcagca cctgctcaac ctgcgcctgc tggcgtttca
     2461 ggtcttcacc cgcagagccc ataacgtcga tcagcggacc aaccagtttg tagaagaagg
     2521 tttctttcgc gccgagcata ttgccgtgac gcactgcgcg acgaatgata cgacgcagta
     2581 cataaccacg gttttcattg gacggcatta cgccatccgc gatcaggaac gcacaagaac
     2641 gaatgtggtc agcgattacg cgcagcgatt tattgctcag atcggtcgcg ccagtgactt
     2701 tcgctaccgc ctggatcaac gtgcggaaca ggtcgatgtc atagttagag ttaacgtgtt
     2761 gcagcaccgc agcaatacgc tccagaccca taccggtatc tacagacggc ttcggcagcg
     2821 gttccatcgt gccatctgcc tggcggttga actgcatgaa gacgatgttc cagatctcaa
     2881 tgtagcggtc gccgtcttct tccggacttc ccggagggcc accccaaatg tggtcgccgt
     2941 gatcgtagaa gatttcggtg cacgggccgc acggaccagt gtcacccatc tgccagaagt
     3001 tgtcagatgc gtatggcgca cctttgttat cgccgatgcg aataatacgt tcgcgcggga
     3061 tcccgacttc tttttcccag atttcgtagg cttcgtcgtc gctttcatag acggtaaccc
     3121 acagacgctc tttcggcagg gcaaaccatt tttcgctggt cagcagttcc catgcaaact
     3181 gaatggcatc gtgtttgaaa tagtcgccga agctgaagtt gcccagcatt tcgaagaagg
     3241 tatggtgacg cgcggtgtaa ccgacgtttt ccaggtcgtt gtgtttacca cccgcacgca
     3301 cgcagcgttg ggaagtggta gcgcgggaat aattacgctt gtcgagccca aggaacacat
     3361 ccttgaactg gttcatcccg gcgttggtaa acaacaaagt tgggtcgtta tggggtacca
     3421 gggagctgct ggcaactacc tgatgtccct tactatggaa aaagtcgaga aacgcctgac
     3481 ggatctcagc ggtgctcttg ctcataatta tcctgaaatc aagctaacga aatatcgcca
     3541 ccagctccag cgtgtcttaa ccgccgggct ggtaactgaa aagtgggaat aagataagtt
     3601 ttcttgactg ggaagtaaaa taccgtatgc gttcagtcgg caaaatttcg ccaaatatcc
     3661 tggatatctt ccatcagata gccacgatag agcagaaaac gctggatctt aactttttct
     3721 gaaaagacag ttggcaaagg ttcaccatat tttcgcgtcg cctgatcgcg cgccagtgcg
     3781 caccagtcga tgtcacattc acgcatcgct ttttctgtcg cttcgcggga aatacctttc
     3841 tgattcagtt cctgacgaat acgtgcaggt ccataccctt tgcggctacg gctggcgata
     3901 aagcgcgcaa caaatcggct gtcatcgaga tagccatgtt catggcacca ggcaataacg
     3961 cgctcgtaat cttctgccgt agcatcaatc tcttctgggc catttttgcc cataatcggt
     4021 gccgcgagtt tacgtcgcag ttcttgctca ctgtgatcgc gcaccgccag aatgcgtacc
     4081 gcacgatcca acaggcgagc atatgccggg cgacgggatg ttgattctgt catggcatat
     4141 ccttacaact taaaaaagca aaagggccgc agatgcgccc caggttttgc tgataaacag
     4201 atttaatagt caatgagtat gcagaatgtt cccgcaggcc caattcgtta gaggatcgag
     4261 gtgagagtaa tttttcacgc cagtgctggc ctgttcccct caccctaacc ctctccccaa
     4321 aggggcgagg ggaccgtccg tgcacacatt atctattatc atcaataacg aacgattaaa
     4381 aatcttcgtt agtttctgct actccttcgc tgtcatctac agagaaatcc ggcgttgagt
     4441 tcgggttgct cagcagcaac tcacgtactt tcttctcgat ctctttcgcg gtttccgggt
     4501 tatctttcag ccaggcagtt gcattcgctt taccctgacc gatcttctca cctttgtagc
     4561 tgtaccacgc gcctgctttc tcgatcagct tctcttttac gcccaggtca accagttcgc
     4621 cgtagaagtt gataccttcg ccgtagagga tctgaaattc agcctgttta aacggtgcag
     4681 cgattttgtt cttcaccact ttcacgcggg tttcgctacc caccacgttt tcgccctctt
     4741 tcaccgcgcc gatacgacgg atgtcgagac gaacagaggc gtagaatttc agcgcgttac
     4801 caccggtagt ggtttccggg ttaccgaaca tcacaccaat tttcatacgg atctggttga
     4861 tgaagatcag cagcgtgttg gactgcttca ggttacccgc cagcttacgc atcgcctggc
     4921 tcatcatacg tgccgcaagg cccatgtgag agtcgccgat ttcgccttcg atttccgctt
     4981 tcggcgtcag tgccgccacg gagtcaacga cgataacgtc tactgcgcca gaacgcgcca
     5041 gggcgtcaca gatttccagt gcctgctcgc cggtatccgg ctgggagcac agcaggttat
     5101 cgatatcgac gcccagttta cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa
     5161 acgcacaggt tttaccttca cgctgcgctg cggcgatcac ctgcagcgtc agcgtggttt
     5221 taccggaaga ttccggtccg tagatttcga cgatacggcc catcggcaga ccacctgccc
     5281 caagcgcgat atccagtgaa agcgaaccgg tagagatggt ttccacatcc atggaacggt
     5341 cttcacccag gcgcatgatg gagcctttac caaattgttt ctcaatctgg cccagtgctg
     5401 ccgccaacgc tttctgtttg ttttcgtcga tagccatttt tactcctgtc atgccgggta
     5461 ataccggata gtcaatatgt tctgttgaaa caattatact gtatgctcat acagtatcaa
     5521 gtgttttgta gaaattgttg ccacaaggtc tgcaatgcat acgcagtagc ctgacgacgc
     5581 accgcatcac ggtcgccgct gaagcattcc cgccgggtaa tgccttcacc gcgggcagtg
     5641 gcaaaagcaa accagacggt gccgacaggc ttctcttcac tgccgccatc cggcccggcg
     5701 ataccactaa tagacacggc ataatcagca cgagccgctt tcagtgcacc tatcgccatt
     5761 tccaccacga cgggttcact caccgcgcca tgctgcgcca gcgtctcttc gcgtacgccg
     5821 atcatctgcg ctttggcttc gttactgtag gtgacaaatc cgcgttcaaa ccaggcggag
     5881 ctaccggcaa tatcggtaat cactttcgct acccaaccac cggtacaaga ctcggcagtt
     5941 gttacggttg cgccacgggc tttcagcgcc tgcccaacct gttcgcttaa ctgcatcagt
     6001 tcactgtcag tcatcacaac ctctgtcagt caaaaattta tgccggacaa gatagcactt
     6061 tcgtcaggaa gatggtgttg gcgttgcaat tttacgagga ggattcagaa aaaagctgat
     6121 tagccagagg gaagctcacg cccccctctt gtaaatagct actgtactcg cgccagcgcc
     6181 acggcttgtc ctaactgcca gaccgccatt gcgtaatggg tgctgtggtt gtaacgggtg
     6241 atggtgtaga agttcggcag accgtaccag tactggtagc cggtgccaac atccagacgc
     6301 agcaggctgg cttgttgatg gttgcccagc ggctgctgtg gcgttaaacc tgcggcggca
     6361 agctgcgaaa tgctgtactt agttttgaaa ccatttggca agcctggagc ctgaccgttt
     6421 gccattatcg cgacctgatc gcctttcacc cagccgtgcg ctttgaaata gttcgccacg
     6481 ctaccgatcg catcaaccgg atcccacagg ttgatatgcc cgtcgccgct gaaatctacc
     6541 gcatattgtt tgtaagacga cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa
     6601 cctttcagat tgagcggatc gtcctgctcg tcgcgcgcca tcagcaggaa ggtttccagc
     6661 tcgccagaga aatactccgc gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg
     6721 aggatgcgag ttttccccat cacgcgcccc cagcgggttt caacgccgat aatcccgacg
     6781 ataatttccg gcggtacgcc atacacctgc cacgcgcgat tcaacgcatc ttcatactga
     6841 ttccagaaaa ccacaccgtt ctgcacgttg tccggcgtaa taaatttttt gcgataacgc
     6901 agccatgcgc cgttcggacc tgatggcggt ttcaccgatg tggttggtgc ctggttatcc
     6961 atcagccgca gtaccgaatc cagacgcttc gcctgggaga gaatttcctg caactgctga
     7021 cgatcgaaac cgtgtttgtt caccattttg tcgatgaact gctgggcatt cgggttatta
     7081 gcgaaatcgc cgcccatctg catcacattg tgctgcggct caagcaggaa gccgccagac
     7141 ggcgttccgg tggtcgtttc agtctcagta ggttttggct tgctgctaca ggcggcaagc
     7201 aacacaaaaa ggggaagcaa tgttacataa cgacgcttga acatgagggg tccatttaac
     7261 agattcaacc aggggcaagt atggtaaagc atcacacccc gcacaaggaa gcggtagtca
     7321 ctgcccgata cggactttac ataactcaac tcattcccct cgctatactt ttattcaaac
     7381 tttcaaatta aaatatttat ctttcatttt acgatcaaaa taacactttt aaacctttca
     7441 acctgattag attaggttgc cgtttggtaa taaaacaata aatcctgaag gagagaacaa
     7501 tgatagaaac cattactcat ggtgcagagt ggtttatcgg gctgttccaa aagggcggag
     7561 aggtgtttac cgggatggtg accggcattc ttccgctgtt gattagcctg caggttatca
     7621 tgaacgcact gattaatttt atcggtcagc atcgtattga acgttttgct caacgttgcg
     7681 ccggtaaccc tgtttcccgt tacctactgt taccgtgcat tggcacgttt gtcttttgca
     7741 atccgatgac cttaagcctg ggtcgcttta tgccggaaaa gtacaaaccc agctactacg
     7801 cggcggcctc ttatagctgc cactcaatga atggcctctt cccccatatc aaccctgcga
     7861 actgtttgtt tatcttggca ttgccagcgg tctgacaacg ctgaacctgc cacttggccc
     7921 actggcggtg agttatctgc tggttggtct ggtcaccaat ttcttccgcg gctgggtgac
     7981 cgatctgacc accgccattt ttgagaaaaa gatgggcatt caacttgaac aaaaagttca
     8041 cctggcagga gcaacatcat gacgcgtatt cggatcgaaa aaggaacggg tggctggggc
     8101 ggcccgcttg agctggaagc cacgccgggc aaaaaaatcg tctatatcac cgccggtacc
     8161 cggcctgcga ttgttgacaa actggcacaa cttactggct ggcaggctat tgacggattt
     8221 aaagaaggtg aacccgcgga ggcggaaatt ggtgtcgcgg taatcgactg tggcggcaca
     8281 ttacgctgcg gcatctatcc gaaacgacgt attcccacca ttaatatcca ctcgacgggc
     8341 aagtccggtc cgctggcgca gtacattgtg gaagatattt atgtctctgg cgtaaaagaa
     8401 gaaaacatca ctgtagtggg tgatgcgaca ccacaaccct cttccgtggg ccgtgactat
     8461 gacaccagta agaaaatcac cgaacaaagc gatggtttac tggcgaaggt gggaatgggc
     8521 atggggtccg ccgttgccgt gttgtttcag tctggtcgtg acaccatcga cactgtatta
     8581 aaaaccattc tgccgtttat ggcattcgtc tcggcgctca ttggcatcat tatggcttct
     8641 ggccttggtg actggattgc ccacggtctt gctccgctag cgagccatcc actgggtctg
     8701 gtcatgctgg cgctcatctg ctccttccca ctgctttcac ctttcctcgg cccaggcgca
     8761 gttatcgcac aggttatcgg cgtattgatt ggcgtgcaga ttggtctcgg caatattccg
     8821 ccgcatctgg ctttaccggc actgtttgcc atcaacgcgc aggcggcttg cgacttcatc
     8881 ccggtcggtt tgtcgctggc ggaagcccgt caggacacgg ttcgcgtcgg tgtcccttct
     8941 gtactggtga gccgcttttt aaccggcgcg ccgactgtac tgatcgcctg gtttgtctcc
     9001 ggttttatct atcaatagag gctgaaacat gaccgttatt tatcagacca ccatcacccg
     9061 tatcggcgcg agtgccactg acgccctcag cgaccagatg ctcatcacct ttcgtgaagg
     9121 cgcgccagcg gacctcgaag agtattgctt cattcattgc cacggcgagt tgaaaggtgc
     9181 actccatccc ggtttgcaat tttcactcgg gcagcatcgc tatccggtga ccgctgttgg
     9241 cagcgtggcg gaagacaacc ttcgcgaact gggtcatgtc accctgcgct tcgatggttt
     9301 aaacgaagcg gaatttccgg gcactgtcca tgtggcaggc cctgtacccg acgatatcgc
     9361 gccgggatcg gttttgaagt ttgaatctgt taaggagtaa aaaatgaatc aggttgccgt
     9421 tgtcatcggt ggtgggcaaa ccttaggcgc gttcctgtgc cacggtctgg ctgccgaggg
     9481 gtatcgcgtc gcggttgtcg atattcagag cgacaaagcc gcaaatgtgg cacaagaaat
     9541 taacgccgaa tatggtgaag gtatggcgta cggttttggt gctgacgcca ctagcgagca
     9601 aagcgttctg gcgctctctc gtggggtaga tgaaatcttt ggtcgcgtgg atttgctggt
     9661 ctatagcgcc ggaatagcaa aagcagcctt tatcagcgac ttccagctcg gcgattttga
     9721 ccgttcgcta caagtgaatc tggtgggtta tttcctgtgt gcccgtgaat tttcgcgttt
     9781 gatgatccgc gacgggattc agggtcgcat tattcagatc aactcgaaat ccggcaaagt
     9841 gggcagcaaa cacaactctg gctacagcgc agcgaaattt ggtggcgtcg ggctgactca
     9901 atcactggcg ctggatctgg cggagtacgg cattacggtg cattcactga tgctcggtaa
     9961 cctgctgaaa tcgcggatgt tccagtcact gttgccacaa tacgcgacca agctgggtat
    10021 caaaccggat caagtcgagc agtattacat cgacaaagta ccgctcaaac gcggctgcga
    10081 ttatcaagat gtgctgaata tgctgctgtt ctacgccagt cctaaggcgt cgtactgcac
    10141 cggacagtcg atcaatgtca ccggcggtca ggtgatgttc tgatcaacag cggagatccg
    10201 ttaaggatct ccgtgagact atagaatgcc tgatgcgcta cgctcatcag gcatacagga
    10261 cttccgccac tacattaagg aaaagttatg gtatccgcac tcatcaccgt cgccgttatc
    10321 gcctggtgtg cgcaactggc cttaggcggc tggcaaattt ctcgttttaa ccgtgccttc
    10381 gacacactat gccagcaagg gcgggttggc gtggaccgtt ccagcgggcg ctttaaaccg
    10441 cgggtcgtgg tcgccatcgc gctggacgat cagcagtgca tcgtcgacac cttgtttatg
    10501 aaaggactga ctgtcttcgc ccgaccgcaa aaaattcccg caattaccgg catgcatgcg
    10561 ggtgatttac agcccgatgt gatctttccc catgatccac tatcacagaa tgctctatca
    10621 ttggcgctta aactgaaacg tggataattt cgttgtgaat gttacttgct tgcgaagtta
    10681 tcattttgaa acctaaatca ggtaatcacg cccatgaaac ctcgtcagcg tcaggccgcc
    10741 attctggagt atctgcaaaa gcagggtaaa tgctcggttg aagaattggc gcaatacttt
    10801 gacaccacag gcacaaccat tcgcaaagat ctggtcattc tggaacatgc cggaaccgtc
    10861 attcgtactt atggcggagt ggtgttgaat aaagaggaat ccgatccgcc tatcgatcat
    10921 aaaacgctca tcaacaccca caagaaagag ctgattgcag aagctgccgt tagttttatc
    10981 catgatggcg attcgatcat tcttgatgct ggcagtaccg ttttgcagat tgttcccctg
    11041 ctctcgcgct ttaataacat cacggtgatg accaacagcc tgcatatcgt caatgcgcta
    11101 tccgaactgg ataacgaaca aactatcctg atgccaggcg gaacatttcg caaaaaatcg
    11161 gcctcatttc acgggcaact ggcagagaat gccttcgagc atttcacctt cgataaattg
    11221 tttatgggca ccgacggcat cgatctcaat gcgggcgtaa ccacgttcaa cgaggtttat
    11281 accgtcagta aggcaatgtg caatgccgcg cgcgaagtga ttttgatggc ggactcatca
    11341 aagtttggcc gtaaaagccc caacgtagtt tgcagtcttg aaagcgtcga taagctaatt
    11401 accgacgcag gtatcgatcc ggcgtttcgt caggcgctgg aagagaaagg gatcgatgtg
    11461 atcataaccg gagagagcaa tgagtgaagc actactgaac acgggacgtc agacgttaat
    11521 gctggagttg caggaagcaa gccgtttacc ggaacgtctg ggcgatgatt ttgttcgcgc
    11581 cgccaatatc atcctgcact gcgaaggcaa agtggtggtt tcgggaattg gcaaatcggg
    11641 ccacattggt aagaaaatcg ccgcaacact tgccagtacc ggcacaccgg ctttttttgt
    11701 ccatccggca gaagcactgc acggcgatct ggggatgatt gaaagccgcg atgtgatgct
    11761 gtttatctct tactccggtg gcgcgaagga actggatctg attattccgc gtctggaaga
    11821 taaatctatc gcgctgctgg cgatgaccgg caaaccgacg tcaccgctgg gcctggcggc
    11881 gaaagcggtg ctggatatct ccgtagaacg cgaagcctgc ccgatgcacc ttgcgccgac
    11941 ctccagcacc gtcaataccc tgatgatggg tgacgcgctg gcgatggcgg tcatgcaggc
    12001 gcgcggtttt aatgaagaag attttgcccg ctcccaccca gctggggcac tgggcgctcg
    12061 cttgctgaat aaagtgcatc atctgatgcg tcgtgatgac gctatcccac aggtagcgtt
    12121 aaccgccagc gtgatggatg cgatgctgga actcagccgc accggtctgg ggctggtggc
    12181 ggtatgtgac gctcaacaac aggtacaagg cgtctttacc gacggcgatt tacgtcgctg
    12241 gctggtaggc ggcggcgcac tcaccacgcc agttaatgaa gcgatgacga caggcggcac
    12301 cacattacag gcacaaagtc gcgccatcga cgccaaagaa gttctgatga aacgcaaaat
    12361 cactgccgca ccagtggtag atgaaaacgg caaactcacc ggcgcaatta acctgcagga
    12421 tttctatcag gccgggatta tttaatcctt caatcccaga cgtttcgcca gccgatgcag
    12481 gttggcgacg tcagtttcca gcattcgcgc acaggctgcc cagttgtgat gattttgtgc
    12541 cagtgcctgg cgaatagttt cacgctggaa cgcttctgtc gcttcacgca ggttttgctt
    12601 aacaacgggc accgccgccg cttctggcgg cggcaacgtc acctcaggaa aagcaaaatg
    12661 ttgcgcctca agaatcactt catcgccgtt gcgggtggct ctcgccagca ctaccgcccg
    12721 atgaatagca tgttccagtt cgcgcacgtt tcccggaaaa ctgtagtgtt gcagtaaatt
    12781 tcgcgccccg gcacttaata ccacgcggga gagcccctgc cgcaaacgac actgctcgca
    12841 gaaatacccc gccagcagaa tgacatcatc gccccgctca cgcagcggcg gcaccgaaag
    12901 tggaaacacg ctcaggcgat gaaacaaatc ggcgcggaat cgccctgcca gcacctcttc
    12961 gcgtaagtcg cggttagtcg ccgccagcac gcgcacatcg acccgcaaac tacggtcatc
    13021 gccaacgcgc tgaatatcgc catactgcaa caccctcagc agcttggcct gcaatgccaa
    13081 cgacaactcg ccgatctcat cgagaaacag agtaccgtta tccgccattt caaacttccc
    13141 gctgcggtta ctgatagcgc cagtaaacgc tcctttcaca tgcccgaaca actcactttc
    13201 cgccacactt tccggcagtg cggcacagtt gagatagacc agcggattca ccgcccgtgg
    13261 cgaggcttca tgaatcgctt tcgccaccag ctccttaccg gttcccgtct caccgctgat
    13321 cagaacgttg agatcggacg ccgccacaat ctcaatctct tttttcagtt gcgtcatgcc
    13381 aggggacaag ccaatcatct gcgtctgttt caccgcttca aacggcgtgg catcgcctgg
    13441 cagcatattc tggctttcca gttgttcaat cagcaacgca ttgcttaacg ctcccgccgc
    13501 cagcgcggca atcagccgta actcttcgtc gctgaaaaca tcgaactgat cgggctgcat
    13561 cccgtcgagc gtcagtgcgc cgatcaggtt ttgtccgaca aacaatggca gaccaacgca
    13621 ggcgtgaacc ttcagactct cctgcccagg aatcaaaccg tcatagggat cgggcaattc
    13681 gctgtctgcg ggaaagcgca ccacatcccc ggcgcgggca atcgcttcca gccgtggatg
    13741 cccttccagc gcaaagcgtc taccgagtac atcctttgcc agaccgtcga tggcaagcgg
    13801 aataaactgc cgcgaatcgt aacgtagcaa cgcagacgca tcgcactcca gcacctgacg
    13861 tagcgtggtg atcaggcgct gaaaacgatc ctgatgacca atcccacgct gcaattcgat
    13921 ggcgatattc gccagcacat caacggaaaa actcatcttt gcctcactgt caatttgact
    13981 atagatattg tcatatcgac catttgattg atagtcattt
//
LOCUS       DAZXUK010000094        13801 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZXUK010000094 DAZXUK010000000
VERSION     DAZXUK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13801
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.094"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..453
                     /locus_tag="ACH3L8_002152"
                     /pseudo
     CDS             1..453
                     /locus_tag="ACH3L8_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            513..620
                     /locus_tag="ACH3L8_002153"
     CDS             513..620
                     /locus_tag="ACH3L8_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFT4085260.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(601..1332)
                     /gene="yehW"
                     /locus_tag="ACH3L8_002154"
     CDS             complement(601..1332)
                     /gene="yehW"
                     /locus_tag="ACH3L8_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFT4085261.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1337..2263)
                     /gene="yehX"
                     /locus_tag="ACH3L8_002155"
     CDS             complement(1337..2263)
                     /gene="yehX"
                     /locus_tag="ACH3L8_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFT4085262.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(2256..3413)
                     /gene="yehY"
                     /locus_tag="ACH3L8_002156"
     CDS             complement(2256..3413)
                     /gene="yehY"
                     /locus_tag="ACH3L8_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFT4085263.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3420..4337)
                     /gene="osmF"
                     /locus_tag="ACH3L8_002157"
     CDS             complement(3420..4337)
                     /gene="osmF"
                     /locus_tag="ACH3L8_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFT4085264.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4589..6886)
                     /gene="bglX"
                     /locus_tag="ACH3L8_002158"
     CDS             complement(4589..6886)
                     /gene="bglX"
                     /locus_tag="ACH3L8_002158"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFT4085265.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7082..8797
                     /gene="dld"
                     /locus_tag="ACH3L8_002159"
     CDS             7082..8797
                     /gene="dld"
                     /locus_tag="ACH3L8_002159"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFT4085266.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8835..9767)
                     /gene="pbpG"
                     /locus_tag="ACH3L8_002160"
     CDS             complement(8835..9767)
                     /gene="pbpG"
                     /locus_tag="ACH3L8_002160"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFT4085267.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9941..10528)
                     /locus_tag="ACH3L8_002161"
     CDS             complement(9941..10528)
                     /locus_tag="ACH3L8_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFT4085268.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10698..11276
                     /locus_tag="ACH3L8_002162"
     CDS             10698..11276
                     /locus_tag="ACH3L8_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFT4085269.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11393..12115)
                     /locus_tag="ACH3L8_002163"
                     /pseudo
     CDS             complement(11393..12115)
                     /locus_tag="ACH3L8_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12207..13643)
                     /gene="mdtQ"
                     /locus_tag="ACH3L8_002164"
                     /pseudo
     CDS             complement(12207..13643)
                     /gene="mdtQ"
                     /locus_tag="ACH3L8_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
BASE COUNT         3275 a         3874 c         3487 g         3165 t
ORIGIN      
        1 gcaatctaca tagcctgcga acgtggatca aagagcgcgg tcaggattac cccgcccaga
       61 cactcaccac acatctgttt attcctctgc gccgacggct tcagtgccaa caaccgactc
      121 tccaggcgct gctggcgatc ctcgacggcg tactgatcaa ctacatcgcc atttgtctgg
      181 cttcggcacg taaaaaacag ggtaaagatg cgctggtggt tggctggaat attcaggata
      241 ccacccgtct gtggctggag ggctggattg ccagtcaaca aggatggcgc attgatgtcc
      301 tcgctcgccc actcgctcaa tcaactacgc cctgaactat tcgaaggccg tacattgctg
      361 gtgtggtgcg gtgaaaatcg aacctccgcc caacagcagc aactcaccag ttggcaagaa
      421 caaggccatg atattttccc actcggtatt taatgattcg ttaacaaatg cgctttactg
      481 tacaatcctt tcgttaacat aaggagtgca ttatgcgcat agctaaaatt ggggtcatcg
      541 ccctgttcct gtttatggcg ttaggcggaa ttggtggcgt catgctcgca ggttatacct
      601 ttattttgcg tgctggctaa gcgcctgcac cagcctttca aacaggcggt ctgcgatgat
      661 cgccgccagt gccaccagta acgccccctg gatcacatac gcggtattaa atccgctaag
      721 cccgatgatg atgggcgtac ccagcgtact ggcccctacc gttgaggcga tcgtcgccgt
      781 accaatgttg ataatcaccg aagttcgcac gcccgccaga atcaccggag ccgccagcgg
      841 tagctcgacc ttacgcagtc gctgacctcg actcattccc atacctttcg caacttctgt
      901 cacgctggga tcaatcgctc ccagcccggc aagtgtcacc tgcaggatgg gcagcacacc
      961 gtaaaggatc aaggcgataa tcgctggttg cagaccaaag ccgatcaccg gaacggcaat
     1021 cgccagcact gcgacaggcg gaaaagtctg tccaacggcg gcaatagttt ccaccagtgg
     1081 gcgaaattcc gcgccccacg ggcgagtaac agcaattccg gcacctgtgc caatgatcac
     1141 cgcaaacaaa ctcgaaattc ccaccagcca gaaatgagcc agtgccagag ctgcaaaact
     1201 ttcttgctga taaacgggtc gtggcagttg tgggaacaag gcagcaaaca gcggctggct
     1261 gtaaggcagc cagaaaatca gcgccacaaa aagagcaatg agccagaaca gcggatcgcg
     1321 caacatcttc atacgcttac gcctccacca gcagatcctg aaaatgcagc gtgccgcaag
     1381 gctggccctg cgtgttcacc accggcagca cctcgcatcc ccgcgcgaca aacagggaga
     1441 gcgcatcgcg tagcgtcatc tcttctgcca gtgcctcacc ttctgcccgt tcttcgcgac
     1501 gcacgtaatc cgccacgcta cgtaacgaaa gcaggtgcac ccccagttca ctacgtccaa
     1561 aaaactggcg gacaaaatca ttcgccggac gagtcagcat cgtcagcgga ttcccctgct
     1621 gcaccacttc accgtgatcc atcaatacca gatgttctgc cagccgtagc gcctcatcaa
     1681 tatcatgagt gaccagcaca atagtgcgcc ccagcaaacg gtgaatgcgc gtcatctctt
     1741 gttgcaacgc gccgcgcgtt accgggtcca gtgcgccaaa aggttcatcc atcagtaaga
     1801 cttgcggatc ggcagccagt gcacgcgcca ctcccacacg ttgctgctga ccaccggaaa
     1861 gctgatgcgg ataacgctca cgcaaatttg actccagccc cagtagcgcc attaattcgt
     1921 cgatacgatc gtcaatccgc gcccgcgacc atttttgtaa ttgcggcacg gtagcgatgt
     1981 tttgcgccac gctccagtgg gggaacaggc caatagattg aatggcatag cccatccggc
     2041 ggcgcaactc cagtactggc agcgagcgaa tttcttctcc ggcaaagcgg atcactccgc
     2101 tgtcatgctc caccaggcgg ttaatcattt tcagggtggt ggatttgccg gagccagatg
     2161 tgccaatcag caccgaaaaa ctcccttcct gaaaattgag attgagatcg ttaacggctt
     2221 tttgtgcgcc gaacagtttg ctgacatggc taaattcaat cattacgttt caccttcagc
     2281 agtgcgataa gcaaatcgaa cagcgcgtcg gtaagcaccg ccagaacaat taccgggatc
     2341 acccccagca acactaaatc aatggcgctg cttagcagcc cctggaaaac cagcgcacca
     2401 aaaccgcctg cgccgattaa cgccgcaatc accgccatac ctacagtttg caccatcacc
     2461 acccgcaggc tgcgcagaaa taccggtaac gccagcggta actgaacatg caggaaacgc
     2521 tgcgccccgc tcatccccat cgctctggcg ctctccagca catcgcgcgg gatctggttc
     2581 aaaccgacta ccacgcctcg caccagcggc agcaaggcat agagcaccag cgcaatcagt
     2641 gcgggtgtca ttccggtccc tgctatgccg agcttcccca gccacggaaa ggccgtaacc
     2701 agcgcggcaa gcggcgcaat caacaggcca aagagcgcca ccgaaggcac ggtctgaatg
     2761 acattgagca gagaaaaaat tgccccctgc cgcgcagtgg aaaagtagca ccagatgccc
     2821 aacggcacac caatcactaa cgcaggcagc accgcaccaa acagcaacgt cagatgttgt
     2881 gccagcgcgt cgtcaaacac atcctgacgg ttggcgtatt ctttcattag tgagagatcg
     2941 ttaagcgtgc cggagtacag caaccacagc ggaataatgg caatctgcat atgcaacaac
     3001 cagcgccaca gcggatgcgt ggagattcgg cggatggcat cgctacaggc cagcaatgcc
     3061 agcgccgcag ccagccagaa acctctgccg aggctggtac gcgccagcgc actgccattt
     3121 tgcgccagtt gggtcgccgc ctttccagct ccccacacca acaatacgaa gacgaattgc
     3181 gccagaatga gtgcaaaaat gctgcctttt ttagcgggga caaaacaggc tgtcagccag
     3241 gcgcaaccaa cgcccaccag catccagatc gtttgcggcc acagttgcca gagatgaagc
     3301 ccttcacccg agactaaacg attaggcgcg taactgataa acggcagcgc cgctgcgatt
     3361 gccgtcagca acaacagcag cgccagaaca ggattaatac gtagataagt cacgggaaat
     3421 tacttcgccc accctttttg tttcaggtag tcggcagcca cttttttggc atccagtcct
     3481 tccactgcaa tgctggcatt cagttgctgc aatgtttttg catcgaggct ggcgaagact
     3541 ggctgtagcc actgtgccat ttgcggatac tccttcaaca ccgattcacg caccactggt
     3601 gcaggcgcgt agataggttg cacgccttgc ggatcgctta aggtttgcag ccccagcgcc
     3661 gcgaccggac cgtcagtgcc gtaagccatt gcggcattaa cgccagaggt ttgctgggca
     3721 gcggctttaa tcgtcaccgc cgtgtcgcca ccagccagtg acagcaactg atcctgaccg
     3781 agcttaaagc cataggcttt ttcaaacgtg ggtaacgcat cggcgcgttc gataaactct
     3841 gccgaggctg ccagtttgaa ggtgccgccc tcttgcagat aacgactcag gtcggcaagc
     3901 gaagtgagtt tgtttttctc tgccacatcc tgacgcacgg cgatagtcca ggtgttattt
     3961 gcaggcgcgg gcgtcagcca gattaacttg ttgtgctccg catcgagctt tttgactttc
     4021 tcgtagccct gcccggcatt tttccacgct gcatcgtttt catctttaaa gaagaaagcg
     4081 ccattgccgg tatattccgg atagatatcc agttcatcgg aagtaatcgc cccgcgcccc
     4141 acaggagtcg ttccgagttg cactttattc accgtcggta cgccgtggct ttcgagtacc
     4201 tgcaagatga tgttgccgag cagcgcgcct tcggtatcga tttttgaacc gactttaacg
     4261 ggggaagccg cctgtagcgg caggctcacg gctgccaaca taaccagtga acctgcccag
     4321 agctttaaga gtggcatgat gctttcctca ttcttttact gttgttttca gcgaattaag
     4381 agaaaagcat agttgataat gacagagtta gcctgagcgg aaggattcag ttgcagaatc
     4441 agataaatac ttaagaggca tattcggtcc ggcgtttcct gccggatgcg gcgcgagcgc
     4501 cttatccggc ctacaaaggg cgaaaattca gcaagttaag atatgaacat caggcaaagg
     4561 gcgcaaacgt cgcgccctta ctaaagcatt acagcaactc aaactcgcct ttcttaacgc
     4621 gtgcggaatc agtgccgata aatacattga acttgccagg ctcggcgtca tatttcatct
     4681 gttgattcca gaacttcagc gcctcaatat cgatcgggaa gctgacagtc tgagtttcgc
     4741 ccggtttcag ggtgattttc tcaaagcctt tcagctgttt cacagggcga ctcatggaag
     4801 ccgtcacatc ctgcaagtac atctgcacta ccgtggcccc ctcgcgctta ccggtgttcg
     4861 tcacctgcac gctggccgtc actttgccgt cacgcttcat ggtcggcgca gaaagtttca
     4921 catcagagac ggtgaaagtg gtatagctca gaccatagcc gaacggataa agcgctccgt
     4981 tagcttcatc aaaataacgc gaagtatatt tgttcggctt gtcggcatta tacggacgac
     5041 cggtgttcag atggctgtag tacaccggga tctgcccgac agaacgcggg aaggaaattg
     5101 gcagcttgcc ggacgggttg taatcgccaa acaatacatc ggcaattgca ttaccgcctt
     5161 cagtccccgc aaaccaggtt tccagaatcg catcagcctg ctgatcttct ttcaccagcg
     5221 ccagcggacg cccgttcatc agcaccagca ccagcggttt accggtggct ttcagcgccg
     5281 caatcaagtc acgttggcct tgcggaatag tgatatcggt ccggctggag gcttcgtgcg
     5341 ccatcccctg tgcttcaccg actacagcca ccaccacatc agattgtttc gccgtctgca
     5401 ccgcttcatc aatcatctct tgcggcgaac gcggatcgac tttgaccgct tcttcatact
     5461 gattcaggaa atcgataatg cctttgtcac tggtaacgtt cgcccctttg gcatacagca
     5521 ctttaccgtt ttcaccgacg gaatttttaa tcccggtcag tacggtcacg gattgatcgg
     5581 caacaccggc tgcggaccag ctgcccatca cgtcacgttt actgtccgcc agtggcccaa
     5641 ccaccgcaat ggtggccgat tttttcagcg gtaacgtttc gagacggttt ttcagcaaca
     5701 ccaggctttc gcgcgccact tcacgcgctt ctttacggtg caggcggctt tcggcattgg
     5761 tatccaccgg gtcagactct ttcggcccca aatggctgta tgggtcgtta aacaacccca
     5821 tatcatattt aacgttcagt acatggcggg cagcatcgtc cagctctgcc atggtcactt
     5881 tgccggattt aatcaaccca ggcagatact tcgagtagta ctcgtcgctc atactcatgt
     5941 tgattccgga tttcagcgcc acgcgcaccg catcttccgg gtctgccgcc gtgccatgtt
     6001 taatcagctc tttgattgca ccgtgatcgg aaacggtgat gcctttaaag ccccactggt
     6061 cgcgcagaac atctttcagc agccaggaat cggaggtggc tggcgtgccg ttcagcgagt
     6121 tcagcgccac catcaccgcg ccgctgcctg cgtccagccc cgctttgtac ggcggcatat
     6181 aatcattaaa caggcgctgc ggactcatat cgacggtgtt gtactcttta ccgccttcta
     6241 ccgcgccgta tgcggcaaag tgtttgacgc tggtcatcac cgagtaacga tctgccgggc
     6301 ttttaccctg catcgcttcc accatggttt tacccattat tgaggtgaga tacgtatctt
     6361 cgccaaaacc ttcggaagca cgtccccagc gcggatcgcg cgagacatcg accatcggtg
     6421 cccaggtcat attcaggcca tcatctgccg cttcataagc agagacacgc ccgaccgttt
     6481 tcaccgcatc gaggttaaaa gacgaggcca gaccgaggct aatcgggaac accgtgcgct
     6541 gaccgtgcag cacgtcgtaa gcaaagaaaa gaggaatttt caggcggctt aattccatca
     6601 cctgatcctg catggcgcgg atatcctgac gggttacggt gttgaaaatc gccccaacct
     6661 gaccgtcttt gatcatctcg cggatcgcct ctttcggatt atccgggcct acgctgatta
     6721 agcgcagctg accaattttc tcatcaaccg tcattttctt gagcagatcg gtgacgaacg
     6781 catcccgcgc ttcaggcgtt aatggatggt taccgaataa atcatccgcc agtgctggct
     6841 gcaggaccag actcaccgcg attcctactg aacatagcca tttcatatgg atttttcttc
     6901 ctttgttgcc cgacgtggca gcgaaaatgg tgcaaaaacc gtagtttgcc ataagcatga
     6961 tggagagaga aaaagaatgc tcagtttatt gtctgaattt tcaaaatatt cactcgctga
     7021 attgtcatac aaggcgctat tctagtttgt gatatttttt cgccaccaca aggagtggaa
     7081 aatgtcttcc atgacaacaa ctgataataa agcctttttg aatgaacttg cccgtctggt
     7141 cggtcattca cacctgctca ccgatcccgc aaaaacggcc cgctatcgca agggcttccg
     7201 ttctggtcag ggcgacgcgc tggctgtcgt tttccctggc tcactactgg aattgtggcg
     7261 ggtgctgaaa gcctgcgtca ccgccgacaa aattattctg atgcaggctg ccaatacagg
     7321 cctgaccgaa ggatcgacgc caaacggtaa cgactatgat cgcgatatcg tgatcatcag
     7381 caccctgcgt ctcgacaagc tgcacgttct cggcaagggc gaacaggtgc tggcctatcc
     7441 gggcaccacg ctctattcgc tggaaaaagc cctcaaaccg ctgggacgcg aaccgcattc
     7501 ggtgattgga tcatcgtgta taggcgcatc ggtcatcggc ggtatttgta acaactccgg
     7561 cggctcgctg gtgcaacgtg gcccggcgta taccgaaatg tcgttattcg cgcgtataga
     7621 tgaagatggc aaactgacgc tggtgaacca tctggggatt gatctgggcg aaacgccgga
     7681 gcagatcctt agcaagctgg atgacgatcg catcaaagat gacgatgtgc gtcacgatgg
     7741 tcgtcacgcc cacgattatg actatgtcca ccgcgttcgt gatattgaag ccgacacgcc
     7801 cgcacgttat aacgccgatc cggatcggtt atttgaatct tctggttgcg ccgggaagct
     7861 ggccgtcttt gcggtacgtc ttgatacctt cgaagcggaa aaaaatcagc aggtgtttta
     7921 tatcggcacc aaccagccgg aagtgctgac cgaaatccgc cgtcatattc tggctaactt
     7981 cgaaaatctg ccggttgccg gggaatatat gcaccgggat atctacgata ttgcggaaaa
     8041 atacggcaaa gacaccttcc tgatgattga taagttaggc accgacaaga tgccgttctt
     8101 ctttaatctc aagggacgca ccgatgcgat gctggagaaa gtgaaattct tccgtccgca
     8161 ttttactgac cgtgcaatgc aaaaattcgg tcacctgttc cccagccatt taccgccgcg
     8221 catgaaaaac tggcgcgata aatacgagca tcatctgctg ttaaaaatgg caggcgatgg
     8281 cgtcggtgaa gccaaatcgt ggctggtgga ttatttcaaa caggccgagg gcgatttctt
     8341 tgtctgtacg ccggaggaag gtagcaaagc gtttttacac cgtttcgccg ctgcgggcgc
     8401 agcaattcgt tatcaggcgg tgcattccga tgaagtcgaa gacattctgg cactggatat
     8461 cgctctgcgg cgtaacgaca ccgagtggta tgagcattta ccgccggaga tcgacagcca
     8521 gctggtgcac aagctctatt acggccattt tatgtgctat gtcttccatc aggattacat
     8581 agtgaaaaaa ggcgtggatg tgcatgcgtt aaaagaacag atgctggaac tgctacagca
     8641 gcgcggcgcg cagtaccctg ccgagcataa cgtcggtcat ttgtataaag caccggagac
     8701 gttgcagaag ttctatcgcg agaatgatcc gaccaatagc atgaatccgg ggatcggtaa
     8761 aaccagtaaa cggaaaaact ggcaggaagt ggagtaaaaa ttacggatgg cagagtatcg
     8821 ccatccgaat tcacttaatc gttctgtgcc gtctgccccg ccgccgccat ttgggcggct
     8881 ttttgttttt tatagctcaa cgctgctgcc ggcacaggca tcactttacc ggtttcaatc
     8941 caggtacgca ggcggctggc atcggcaaaa tgggtatatt tgccaaacgc gtccatcact
     9001 accagcgcca ccggtttatt attgataacc gtacgcatca ccagacaatg gcccgccgca
     9061 ttggtaaagc cggttttggt taactgaata ttccagttat cgcgatacac cagatgatta
     9121 gtattgcgga acggcagcgt atacgtcggg ttagagaagg ttgccatatc ttcccgggta
     9181 gtacttaact gcccgatcaa cggatattgt ttgctggcaa tgagcagttt ggttaagtca
     9241 cgggcagttg aaacgttatg caccgacaat ccggtaggtt caacaaagcg cgtgttgttc
     9301 attccgagcg atttcgcttt cgcattcatt gccttaataa aggctttgta accaccggga
     9361 taatggtgcg caaggcttgc cgccgcgcgg ttttctgaag acatcagcgc cagcaacagc
     9421 atatctttac ggctgatttc gctattcagt cgtacgcgcg aatagacccc tttcatctcc
     9481 ggcgtctggc tgatatccac ttttagtttt tcatccagcg gcagtcgtgc atccagcaca
     9541 accatcgcgg tcattaattt gctgatagac gcaatcggac gcaccagatc cgggtggttc
     9601 gaatagatca ctttgttggt attcagatca acaatcatcg cgctaccgga ggcaatttcc
     9661 ggttgtgaag cggtggtagc ggctgccgtt ttcgcaacgg cctgcggtgc aaaaggcaca
     9721 gccagcatca gggccaagct aaataaagaa actcgaaatt tcggcatgat gagcattcag
     9781 atagtggttc acgcgcacgg gttgcgcacc gccggagtaa ggatttactg aggctagcga
     9841 cgccatcata acgagcaaaa agtgcgatcg tcaaaggaga atcgtgagta aatgctgcat
     9901 tgctgacatt tacgccagca atgcaacgtc aaagaacttt ctagaacaaa cgataaccgt
     9961 agccccatag tataacggtt agggtgagca gcacttccag taccagcacg ccaatcgcca
    10021 gcgtcgaact ggagaagcta aggccttcct ctttgttgat attcaggaag ctcggaatac
    10081 caaggtaaag caggtagccg gtgtaaaaca gcgccaccgt gccgaccagc gcacacaacc
    10141 agaccagtgg ataaagcgcc accagaccgc ttaaaaacag cggagttgca acgtagcccg
    10201 cgaagaccat gcagtgcgca agagacggac gctgcgggta attgcgcgcc atccaccaga
    10261 tgacccgccc catcaccgca accccagcca gcataacgcc gtaaaacaag acagccagcg
    10321 ccagtccggt aaaccaggat aacttcagga tagtgccatc gccaaaattc cagccaatct
    10381 gtgtagtgcc aatgaaggcg caaatcaccg gaatcgccgc catcagcaaa acgtggtggg
    10441 tgtaatggtg agaaatcgtt tcgttttcgc gattaatcac ctgcatttca cgatcgggat
    10501 gggaaaacag tccccagaca tggctcatac cgcccccttg ttgtgagttc atgaacctga
    10561 cagttcaagt ataagccagc ttgtgattat tttttgttca tccatgtaat ttgcaccgtc
    10621 tgatgtatgt cctttttcag cgtgaatgat tcacagggtg tatgcttaca ggcaaccaaa
    10681 gggagacaaa ctggcctatg gatctcaata cactcatctc acaatatggt tatgccgcgc
    10741 tggttatcgg tagcctggcg gaaggtgaaa ccgtgacttt gctgggaggc gttgcggcac
    10801 atcaggggct attaaagttc ccactggtgg tactttctgt ggcgcttggt ggcatgattg
    10861 gcgaccaggt gctctatctg tgcgggcggc ggtttggcgg caagctgtta cgccgtttct
    10921 cgaaacatca ggataaaatt gagcgggcac agaaacttat ccaacgccat ccgtatctgt
    10981 ttgtcattgg tacgcgcttt atgtatggct ttcgggtgat tggcccgacg ctgattggtg
    11041 ccagccagct gccgccgaaa atctttctgc cgctgaatat tctcggcgca tttgcctggg
    11101 cgttgatttt taccactatt ggttacgctg gtggtcaggt gattgcgccg tggttgcaca
    11161 atctcgacca gcatttgaag cactgggtct ggttgattct ggtcgtggtt ctggtggtgg
    11221 gcgtgcgctg gtggctgaaa cggcgcggga agaaaaagcc ggataatcag gcgtaaagca
    11281 gttgccacag ataagcattc actccacatc cggcaccggg tgttccatgc ctgatgcgac
    11341 gctggtgtct tatctggcct aacccattga aatatccacg cacctcactg cgctattctg
    11401 ggttgaactg cggattcgcc aacataaagc cgccatccac tatcaacgac tgccctgtcg
    11461 tgtaatttgc gccctccgaa caaagccaca ccaccaggct ggcaatctca tgcgttgcgc
    11521 caaaacgccg tagaggaatc gaaggctccg cgtcgggctt cacgtcgcta tcatccatgc
    11581 tgttcattgg cgtggctatc gccccaggcg cgactgcgtt caccaaaatc ttatgcctga
    11641 ccaactccag cgccatcgtt ttggttaacc cgccgagcgc atgtttagcg gctgtgtagg
    11701 cgctggcatc cggcagcggc gtatgttcat gtaccgacgt aatgttgatg atgcgaccgc
    11761 cctgccctta tttcaccatc tgacgagccg caatttgcga gcataaaaat gcaccatcaa
    11821 catcaacggt aaaaatcttg cgccactcat caaaagccat atcaagaaac ggcgctttgg
    11881 tcattgcacc cgcattattc accagcacat caatacgccc aaatcgctgg atgagtgtct
    11941 ccagcgcctg cgcaccttct ggcagattgc cgagatccag ttgcacgatc tccgcacgta
    12001 cgccgtgggt aaccacctca cgcgcggtat cttttgcccc ttcttcatct gagtgccagg
    12061 taatgccaat atcaaacccc tgctgcgcca gtaataacgc gcactctttg ccgatccccg
    12121 aatcggaagc ggtaattatc gcgacctatg ccatcgagtt ctccacttaa cgctgaataa
    12181 acgttaagta tagaaggcgc atatcatcag cgtttgtaac ccccgcccag cgcaccggtg
    12241 agtcgaatgg aggcatccag ccactgccct tgcaataaca ggccattggc ccgctcacgc
    12301 agcgccggga ttctggcttc gctgacgcgg gaaccagcaa tgatgcccgc gttaaagcgg
    12361 gcctgcgcaa gacctaccac acgcaaggca tcgcgctcaa tttgcgcctg atgctggttt
    12421 ttctccgcca gtgtctgaac ctgactggct gcccgcgcca cgtcattcac tgcttcaacc
    12481 accgctttgt tgtagctggc gatagacaag ttgctttcgg cttttgcgat atcgagattg
    12541 gcgttaagac gaccactatc gaaaataggt agcgtcaggc ctgccgtaac gcccatttgc
    12601 tgcgcggaat gacggaacag atcgcttaag tgcaacgcat cctgttgcag gaaggccatc
    12661 aggttgatgt caggataaaa tgccgctttt gccgcatcaa tagtgcttaa cgatgactca
    12721 acgtaccagt gcgccgcctg caaatctgcc cgccgggcca gtaaggagta ccccagttca
    12781 tcaggaagct ggcttgccac tttcggcaac gcgaccggtt taagcttcaa tgacttagtc
    12841 tggtgatttg tgagtgcatt taaccgtgcc tcaataattt tcattttccc cgcgacatcg
    12901 ttgagctgct gccgggtttt gctggcatta atatcggttt ccacaccgtc aactgaagaa
    12961 gtaatcccgt tctgatatag ctggcgatcg gtcgcgataa tggtgttctg ctctttttct
    13021 atttgctgca agaccgtgtt taacgccgcc tgggtttgcc actcccagta caggcgggct
    13081 acgctgccag ccagcaattg gcgggtttgc tcgcgttccg ccgcccgtgc tttaaccgta
    13141 cccaggcggg cagtaacctc cgcccgattc tttccccaga tatcgagatg ccagcccgcc
    13201 gttaagccaa aagttccgtt ggtgtaccac ggaccggtcg tacctgcggc cggatcgttc
    13261 agagcaaacg gccccattag cccttctgcc gacatttttt gccgctccat atccgccgaa
    13321 aagtcgatct gcggaccatc ctgagtggca actgccttcg cctgggcttc agctagctaa
    13381 atgcgctgtt cagccacctg catatccggt gcgttctgta gtgcattgtt aattaatgaa
    13441 gtgagctgat tatcgtgata ctccagccac cattggctgt ctggccaacc atttttcagc
    13501 gccgtgggta atgcggtgtc aacttgtgca gcgggcgttt gctggcttaa cgcctggcgg
    13561 gtttcatgca taggcgcaca cccggccagc atcagtaaca gcggaaaaca ggcgatggct
    13621 ggataaaagg aatcacgatt catgggggaa taatcaggta agaaacggtg cgcggagatt
    13681 accgtgtgtt gcgatatatt ttttagtttc gcgtggcaat acatcagtag caataaaacg
    13741 acatacccag aaaaatatag actaagtaaa tgatatcttc cgatttatct taatcgttta
    13801 t
//
LOCUS       DAZXUK010000095        13535 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZXUK010000095 DAZXUK010000000
VERSION     DAZXUK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13535
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.095"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            112..1167
                     /gene="phoE"
                     /locus_tag="ACH3L8_002165"
     CDS             112..1167
                     /gene="phoE"
                     /locus_tag="ACH3L8_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFT4085270.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1206..1607)
                     /gene="crl"
                     /locus_tag="ACH3L8_002166"
     CDS             complement(1206..1607)
                     /gene="crl"
                     /locus_tag="ACH3L8_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFT4085271.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="ACH3L8_002167"
     CDS             complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="ACH3L8_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFT4085272.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="ACH3L8_002168"
     CDS             complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="ACH3L8_002168"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFT4085273.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3721..5178
                     /gene="pepD"
                     /locus_tag="ACH3L8_002169"
     CDS             3721..5178
                     /gene="pepD"
                     /locus_tag="ACH3L8_002169"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFT4085274.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5235..5849)
                     /gene="prfH"
                     /locus_tag="ACH3L8_002170"
                     /pseudo
     CDS             complement(5235..5849)
                     /gene="prfH"
                     /locus_tag="ACH3L8_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5911..6954)
                     /locus_tag="ACH3L8_002171"
     CDS             complement(5911..6954)
                     /locus_tag="ACH3L8_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFT4085275.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            complement(7200..7652)
                     /locus_tag="ACH3L8_002172"
     CDS             complement(7200..7652)
                     /locus_tag="ACH3L8_002172"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFT4085276.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="ACH3L8_002173"
     CDS             complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="ACH3L8_002173"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFT4085277.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="ACH3L8_002174"
     CDS             complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="ACH3L8_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFT4085278.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="ACH3L8_002175"
     CDS             complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="ACH3L8_002175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFT4085279.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="ACH3L8_002176"
     CDS             complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="ACH3L8_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFT4085280.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10291..12003
                     /locus_tag="ACH3L8_002177"
                     /pseudo
     CDS             10291..12003
                     /locus_tag="ACH3L8_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="ACH3L8_002178"
     CDS             complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="ACH3L8_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFT4085281.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(12893..>13535)
                     /locus_tag="ACH3L8_002179"
     CDS             complement(12893..>13535)
                     /locus_tag="ACH3L8_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFT4085282.1"
                     /translation="KILTFIQPVYVFHVFVLARQIFASRRVFVMRFAPFTRQRHHNRE
                     QIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGG
                     TRPDQGFDCSGLVFYAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHS
                     REIADHMGVYLGDGQFIESLRTGETIRVSRLAEPFWQDHFLGARRILTEEMIL"
BASE COUNT         3365 a         3514 c         3385 g         3271 t
ORIGIN      
        1 aatatcatta atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca
       61 acggggctct cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag
      121 agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa
      181 atatataata aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
      241 atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt
      301 gaaactcaaa ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga
      361 aataaagcgg agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
      421 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
      481 gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt
      541 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcgttatc
      601 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
      661 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc
      721 attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc
      781 acaggcaagc gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat
      841 ctggcaactt tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat
      901 aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
      961 ctgggttatg tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc
     1021 aattatatcg acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat
     1081 tataaaatca accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc
     1141 gcggttggga tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat
     1201 ttcactcacg ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc
     1261 agattcagcg tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc
     1321 agtcgttcaa ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg
     1381 aacagaccaa actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac
     1441 cagccccaga attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa
     1501 tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta
     1561 aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt
     1621 tgtgatgcaa ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt
     1681 tcgatccagc cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat
     1741 gggatctcta ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc
     1801 gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc
     1861 agcaatcctt gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa
     1921 gcatcatgca tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc
     1981 tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc
     2041 agacgcggcg attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca
     2101 aaggccgcga cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc
     2161 tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc
     2221 atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa
     2281 tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg
     2341 cctttcggca tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc
     2401 tgccgcatcg tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc
     2461 tgcgcttgct cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac
     2521 gtagccgcat gcagccagtg tcgaccactg agttgcggat cttcttccgt acaggctttt
     2581 tgttgccaca ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca
     2641 atcacggtat cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc
     2701 tggacgtcga ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc
     2761 atgcgatacc agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg
     2821 ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac
     2881 agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac
     2941 tattgtaacc tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga
     3001 ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca
     3061 ggtatcttgc gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc
     3121 gaagatggtg acaaagtgcg cttttggata catttcacga atcgcaacag cagtaccacc
     3181 ggtatccacc aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag
     3241 cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg
     3301 acgaataccc agttcacgcg ccagtaacgc ccccggtacc agaccgccac ggcttacggc
     3361 aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat
     3421 ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt
     3481 atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg
     3541 cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga
     3601 cgcgggataa agtggtattc tcaatacata tctcgcaagc ctgtcttgtg ttgacaacat
     3661 tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac
     3721 gtgtctgaac tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt
     3781 tctattcctc acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca
     3841 aaagagaaag gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtcaacct
     3901 gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg
     3961 ccgcagaaaa ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt
     4021 gatggcgaat gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg
     4081 gcctctgcac tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg
     4141 ctgaccatga ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg
     4201 caggctgata ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt
     4261 gcggggggta tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt
     4321 tttgaaacct tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc
     4381 cacgttggcc tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa
     4441 gaactggacc tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt
     4501 gaagcctttg cgaccattgc tgtcgcagct gataaagtcg acgcactgaa atctctggtg
     4561 aatacctatc aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg
     4621 ttgctggact ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt
     4681 attcgtctgc tgaacgccac cccgaacggt gtgatccgca attccgacgt ggcaaaaggt
     4741 gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt
     4801 cactgcctga tccgttcact aatcgacagc ggtaaagact acgtggtgag catgctggat
     4861 tcgctgggta aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag
     4921 ccggacgcta attctccggt gatgcatctg gtacgtgaaa cctatcagcg cctgttcaac
     4981 aagacgccga acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg
     5041 tatccggaaa tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat
     5101 gagcaagttc acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa
     5161 gaaattccgg cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc
     5221 agtttcatcc ttcattatcc ttcgataaaa gccatccctg taaacgttcg tcgcgggttg
     5281 ccacgttcaa tctggtgatg gaacattcgc cgctgtgatt tcagcaccgc gctattttcc
     5341 tattgctgct gatccagctt ccaggcaatc agcaatcgcg ccagccgttt gttagcatgc
     5401 tggctgcgct ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc
     5461 gccgagtcgg ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcatac
     5521 cggatcgcat cggattgctc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag
     5581 tttttacgcc cgtgatgtgg gcgatacggg ctcgggcaaa tccactgaat agtgccacac
     5641 cagctttcgc ttaacgccca tgcgttgtcg ccatccagag aaatcagcgc cgaacgcagc
     5701 gtgtcagagt agcggcctgt ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca
     5761 gcttctttaa ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc
     5821 tgcgctgagg agagttatag caagatcatc cttttttccc tccactgttt ttgagcgtta
     5881 gcaccggacg cagccgcgcg acaggaataa ttaatccggc ctgccctgac attgcaccac
     5941 actttcagcc gatttatagg cttgtggcgc ttcttcgaag atgagttgtt tatcgcgaca
     6001 aattacccga ctgccaagtt cagtacgcga gagttgcgtc gctgtgtatt tcgctgccag
     6061 acgcccttta cactcggtgc gcccccattt acgcccagcc ccatgcgcca gcgaatgcaa
     6121 tgtttcctca ctcctgacgg gctgaaccag cctggagtaa tcaccgcgtg aaccgggaat
     6181 aaccaccaga ccacagtcat caggcgtggc acctttacga tgtaagaccc cttgttgatc
     6241 accgattcga cacgcgctaa caaagttatg cgccacatcc agaaccggac ttccggtggc
     6301 cttaacctgt tgcattatgc gcaaagcgat caggtggcga ttaaaacgcg caaacgccag
     6361 cgcgtcgtca ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa
     6421 aatagactgc cctagccccc tcgagccgct atgaaccagc agttgcagat gctgcgcatc
     6481 cagacctgcc agtgcaaaca gttcagcgtt gatgatttga tcaacttgtt gcagttcagc
     6541 gaaatgattg ccaccaccga tagagccaag cgagctgcgc cacggatgct gtgcaaatgc
     6601 tgacggcagg ttttcctcca gccagctttc ttcagcaacg tcatccaaag ctgataatcg
     6661 cttttcaaac ttatcggcgt tgtatttgcg agcgagaata tctgtttgcc atagcgccat
     6721 gccacagccg atatcattgc cgaccagtgc tgggtaaaaa cggcctgcgg agaagaacgc
     6781 tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta cgcgctgcat
     6841 gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc
     6901 atcagatgcg atggtaaata ccgcatcgga taagggacga atataattgc ccatatacca
     6961 ttccgtaaat agactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttattgagag
     7021 taacaattaa aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc
     7081 ctggaaaggt ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt
     7141 ttgtggaata agaaaacgcg cacatactag gcgggcgtta ttttcattgc aagctggatt
     7201 taatgttgcg gtttataacg catataaaaa ttaataaacc acgctccccg gcattcaacg
     7261 cgctgctgct taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctt
     7321 gcgtccaccg taagttcgga ttcagactta ataaaaggtt ttaacaaagc gctggcaacc
     7381 ccacggcggg tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta
     7441 ataaagccga ccagttttgc attaataact gcaacccgca cttgtgattt cgcgagtttc
     7501 tccttccagc gagattcgtc aatctgcgcc caggcggcaa tttgttgtag tgaataatgc
     7561 tgactgacgg tcatcgtaac cgctctaatg aaaatagcgc atagttgctg aaaatcgcca
     7621 ggctgataat ctcttatttg tatgttattc ataatataaa ttcaaaaacg catgcgaaac
     7681 gcttctgcca tttttccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt
     7741 tcaggcttca gaatggcaat gagcaaccat gcttgctcat caaacgcacc ctgacaatat
     7801 accaaatgcg cttcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta
     7861 ttcacatttg ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac
     7921 gagtcgtcgt agagtgcatc gcgaccaaat atatctggca aaacaccgtc acgcttatag
     7981 gaaataaaat ccgccgttaa cgcatcaagt tcctctgctg taagttgcag gcgaataagt
     8041 tttgttttga atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat
     8101 cattaagata ttgtttagcg cggcgtgtaa tttcctctaa tcttgcagca cttggacgaa
     8161 agcgcgcctt tatgggcttt ttctcctctt gttcagcaag catgtccatt aacgcttcga
     8221 atgcgctggc acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaactggtt
     8281 gatcaataaa gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag
     8341 cgagaattcg atgcatacag tgataccctc ataataatgc acaccagaat atacataata
     8401 gtatacatca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     8461 gcagccccac cagacgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     8521 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgggtg gtctgctgaa
     8581 aatcgtcgaa ctttaatttc accccctggc gagcaatcag taaatcaggt tttacctttg
     8641 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     8701 gatgaatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgtaac cgttcgctgt
     8761 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     8821 agcgtttaag cagaatcacc agatcacact tttgtacatc accgcaggtc cgcagcccca
     8881 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gattttttcc agtggtaagg
     8941 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctgaccgttg ggtttattca
     9001 tgtcggaggc gattttggcg agaaacttta ctggtgccac gcccgcagat gccgtcagtt
     9061 gcagctcgtt gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     9121 aatggacgct atcggtgaca tcgagataag cctcatccag tgacaacggt tcaatgcgcg
     9181 aggtgtagcg cgagaagatt tcacggatat gatttgaggc ttctttgtag gcgtcaaagc
     9241 gccccggaag caaggtgaga tgtgggcata atttgagcgc catcccagta ggcatagcgc
     9301 tacgtacgcc aaatttacgc gcgggataat tggcggtgct gatcaccccc cgacgttcgc
     9361 ggctgccgcc aatagcaata gggatatcgc gcagagcggg attgtcgcgc atctccaccg
     9421 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     9481 tggtaaagta tacagtgatt tcaggttttg agaaatgcgt aaagattcag catttagcgc
     9541 attcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     9601 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     9661 atctcaatac gccgattgcc cgcgctttgc ggattcttcg cgtccagcag catctggtcc
     9721 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     9781 cgagctgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag
     9841 gccatcgcat cggtatgccc ggtaataata attttgttat agagcgagtc gaacactggc
     9901 gcgagctcca ccagcaaagt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     9961 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
    10021 tccagattcg cttccatatg cgcatcacgg gcaatggtgt tgatactggt tgccaactcg
    10081 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattcac atcacgcgct
    10141 tttttctccg tctcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
    10201 attttattta acggcgacag cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg
    10261 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat cgtcgtcacc
    10321 aaagaggccg agcgtatttc cgaggtttct gcccgcttta ccctggacgc gatgcccggc
    10381 aaacagatgg cgattgacgc cgatcttaac gccggattga tcaaccaggc gcaggcgcaa
    10441 acccggcgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
    10501 aagtttgtac gcggagacgc catcgccggg atgatgattc tggcgatcaa cctgagcggc
    10561 ggcgtctgta tcggcatctt caaatacaac ctgagcgccg acgccgcctt ccagcaatac
    10621 gtgctgatga ccatcggcga cggcctggtg gcgcagatcc cttccctgct gctctccacc
    10681 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgc
    10741 caccagttgc tggcaagccc gtcggtgctc tacaccgcca ccgggatcat gttcgtgctg
    10801 gcggtggtgc cggggatgcc gcatctgccg tttttgctgt tcagcgccct gcttggcttt
    10861 accggctggc ggatgagcaa acgcccgcag gcggcggagg cggaagagaa aagcctcgaa
    10921 acgctgaccc gcaccatcac tgaaaccagc gaacaacagg tcagttggga aactattccg
    10981 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcg
    11041 cagggcaacc cgctcaccca gcggattcgc ggcgttcggc aggtgatttc cgacggtaac
    11101 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gcctcaagcc cagccagtac
    11161 gctattttca tcaacggcat tgaggctgat gaagcggata ttccggcgga taaactgatg
    11221 gcgctgccct ccagcgaaac ctacggcgag attgacggcg tgctggggaa cgacccggcg
    11281 tacgggatac cggtcacctg gatccagcct gcgcagaaag cgaaggctct gaatatgggg
    11341 tatcaggtga tcgacagcgc cagtgtgatt gccacccatg tgaacaagat tgtgcgcagc
    11401 tatattcctg atttgtttaa ctatgatgac atcacgcagt tgcataaccg tttgtcgtcg
    11461 atggcaccgc gcctggcgga ggatttaagc gcggcgctca attacagcca attgctgaaa
    11521 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
    11581 gtgctggtcg ccagtagcac ggtgactaaa gatcatattc tgctggcggc cgatgtgcgc
    11641 ctggcgctgc ggcgcagcat tactcatccg ttcgttcgca agcaggagct gacggtgtat
    11701 acgctgaata atgagctgga aaatctgctg accaacgtag tgaatcaggc gcaacaggcc
    11761 ggcaaagtga tgctcgacag cgtgccggtt gacccgaata tgctcaacca gttccagagc
    11821 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagatccggt gctgctggtg
    11881 ccgccgcagc tacgcccttt gctgccgcgt tatgcaaggt tgtttgcgcc ggggctgcat
    11941 gtgctgtcgt ataacgaagt gccggatgag ctggagttga agattatggg ggcgttgatg
    12001 taagaatccg gcatcggtgc acaattgccg gatgcggcgt aaacgcctta tccggcctac
    12061 cgttcaggca caagttttta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
    12121 cataactgcc ggatgcggcg tgaacgcctt atccggccta ccgttaaagc acgatttgta
    12181 ggccggataa gcgtaagcgc atccggcacc agcgcacagt tataaaatga tacgctcccc
    12241 ggcacttaaa tccgttatgt ctcccgccca atcgatggga tataaccctc gcgcgacatc
    12301 gcgatggaac gttgagaatg gccaatcact cacctgcttt acccagccat gtttctctgg
    12361 atttatataa atataatcaa catgatggcg ataatctttt gtattgcgaa tggcatgctc
    12421 ccaaaaacgc ggttgccaga tatttttcaa tccgcaagca tgggtgaact gctttttaat
    12481 ctcccgccag cgagaggaaa aatcatcatc gctttcgggt aatgtccata tgcaatgcat
    12541 atgctccggc aaaacgaccc aggcgttaat ttcaaaaggt ctgtctcgct taacgttaat
    12601 aatggcgtta cggagcgtct ggaactgggt ggtcagaaga tggcttcgac gattgcgtaa
    12661 atttaccgtg aaaaaccatg ttcccccctt gatgtaataa cgacgatatt ccgacatagc
    12721 gactcctttt tctcaacggt aagaggaaaa ggaaaggccg acaatgacgc tgtgttatat
    12781 cgggaaagcg gctcgcaaaa acgcacagat ttgtaggcat gataagacgc gtcagcgtcg
    12841 catcaggcat ccgtgcacaa ccgccggatg cggcgtaaac gccttatccg gcctacaaaa
    12901 tcatctcttc cgtcaaaatc ctgcgcgcgc ctaaaaaatg gtcctgccag aaaggttcgg
    12961 ctaaccggct tacccgaatg gtttccccgg tgcgcagtga ctcaataaac tgaccgtcgc
    13021 ccagatacac ccccatatgg tcggcaatct cgcggctgtg gatatggaaa aatagcaaat
    13081 ctccccggcg caggtcgtta ttcgccacaa tcgttgcccg acggtagtgg tacatctcat
    13141 tggccgtgcg cgggagtttc gcctcaagga tcttgttgta ggcataaaaa actaacccac
    13201 tacaatcgaa gccttgatcg ggccgcgtgc cgccccagac gtaaggcttg ccaagctgct
    13261 gctccaggcg gtgaatcgcc acttcggtga tgttatgcag gtgatcgctg cttaaaaagt
    13321 ggttgttttc cgccagcgcc tgccagcgtc tgtcactcgc cttgagcggc gcaggaaacc
    13381 attctggatg ctgttttatc tgctcccggt tgtggtggcg ctggcgagta aagggagcaa
    13441 aacgcataac gaaaacacgc ctggatgcaa aaatctgccg agcaagaacg aagacatgaa
    13501 agacataaac cggctgaatg aaggtgagaa tttta
//
LOCUS       DAZXUK010000096        13153 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZXUK010000096 DAZXUK010000000
VERSION     DAZXUK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13153
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.096"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(26..133)
                     /gene="rprA"
                     /locus_tag="ACH3L8_002180"
     ncRNA           complement(26..133)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACH3L8_002180"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(319..1431)
                     /gene="ydiK"
                     /locus_tag="ACH3L8_002181"
     CDS             complement(319..1431)
                     /gene="ydiK"
                     /locus_tag="ACH3L8_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFT4085283.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1820..4876
                     /locus_tag="ACH3L8_002182"
     CDS             1820..4876
                     /locus_tag="ACH3L8_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFT4085284.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4873..5283
                     /gene="menI"
                     /locus_tag="ACH3L8_002183"
     CDS             4873..5283
                     /gene="menI"
                     /locus_tag="ACH3L8_002183"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFT4085285.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5383..5571
                     /locus_tag="ACH3L8_002184"
     CDS             5383..5571
                     /locus_tag="ACH3L8_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFT4085286.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            6119..6487
                     /gene="sufA"
                     /locus_tag="ACH3L8_002185"
     CDS             6119..6487
                     /gene="sufA"
                     /locus_tag="ACH3L8_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFT4085287.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            6496..7983
                     /gene="sufB"
                     /locus_tag="ACH3L8_002186"
     CDS             6496..7983
                     /gene="sufB"
                     /locus_tag="ACH3L8_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFT4085288.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7993..8739
                     /gene="sufC"
                     /locus_tag="ACH3L8_002187"
     CDS             7993..8739
                     /gene="sufC"
                     /locus_tag="ACH3L8_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFT4085289.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8714..9985
                     /gene="sufD"
                     /locus_tag="ACH3L8_002188"
     CDS             8714..9985
                     /gene="sufD"
                     /locus_tag="ACH3L8_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFT4085290.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9982..11202
                     /gene="sufS"
                     /locus_tag="ACH3L8_002189"
     CDS             9982..11202
                     /gene="sufS"
                     /locus_tag="ACH3L8_002189"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFT4085291.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            11215..11631
                     /gene="sufE"
                     /locus_tag="ACH3L8_002190"
     CDS             11215..11631
                     /gene="sufE"
                     /locus_tag="ACH3L8_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFT4085292.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11780..12784
                     /gene="ldtE"
                     /locus_tag="ACH3L8_002191"
     CDS             11780..12784
                     /gene="ldtE"
                     /locus_tag="ACH3L8_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFT4085293.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12847..13083)
                     /gene="lpp"
                     /locus_tag="ACH3L8_002192"
     CDS             complement(12847..13083)
                     /gene="lpp"
                     /locus_tag="ACH3L8_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFT4085294.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         3306 a         3246 c         3535 g         3066 t
ORIGIN      
        1 ggcgaggtag cgaagcggaa aaatgttaaa aaaaagccca tcgtgggaga tgggaaaaga
       61 ctacacacag caattcgttg tttcactcag gggatttcca tgcttataaa tcaatatgtt
      121 gatttataac cgtgagctaa tagtaggcat acggactttt tgcgtcgatc agattcgtct
      181 cattagttta aaaactgtaa agaatttgcg acaggtacta ccagctgaat ggcggtaagc
      241 aggaaaatat ggggaagagc aagcaaatat caattagcgg tgattaacaa tcaggcggca
      301 gagccgcctg atgagaaatt acttattcgg tttctcgatt tcgccaagtt cttcgagaat
      361 ttcttccggt tggtccgtcg gtggcgggac ttcttccacc cacgcggcaa acagacgcca
      421 ggaaacggct aacagaaccg gaccaataaa cagaccgatc atcccgaaag caatcaaacc
      481 accaataacg ccagagagga tcaggatcag cggtaaatcg gcacccatgc gaattaacat
      541 tgggcggatg acgttatcca gcgtgccaac cacaccgctc cacactaaca ataccgttcc
      601 ccaggtggta tcgccggtcc agtagagcca gataatcgcc ggaatcagta ccggcaacgg
      661 gccaagctgg acaaggcagg agaggatcat taacaccgtt agcaacgttg cataaggtac
      721 gccggatacg gcgaggccga taccgccaag cactgcctgt actaacgccg tcaccaccac
      781 acccagcgcc accgcgcgga tagcctgtgc cgccagcagc acggcggcat ctccgcgaac
      841 gcctgccaga cgggttgcaa aatggcgaat gccttgtgcc acctgttcac cgcgccagta
      901 cagcagggca ctgaagagaa gcatcagcgc acaatgcacc ataaagcgcc cgatatgcgc
      961 cgcctgccca acgaaccagg tggtggtggt gccaatataa gggcggactt tcgccatgat
     1021 cgccgtaccc cccatatcca gcaagttgtg ccagcctgca tacagcttcg cgccaatcac
     1081 cggaatggta ttaagccacg ccagatcggg taacgtcatg tcgccgctgg aaatggcttt
     1141 aattagcggg ccgctgccgt cgacgatact gttaaccagc aaagcaatag ggatgataaa
     1201 caccatcact aataacagcg tcatcaccag aacggcgaga gagcggcggc caaacatgat
     1261 cttttgcaaa cgtaacaata ccggccaggt ggcgataacc accgtaccgg cccatgcaaa
     1321 gccgagaata aagggttgaa caatccacag acatgccaca atcatgatgg ctaaaaacag
     1381 caccgaaagc agaatttgtg cgacatccct gggctgacga acatttacca taactacttt
     1441 tcaccttttt tgtgcgtcaa atcgtcgacg cgacgtgaac acgcgaaact cacctgcata
     1501 atgatgagca atttcagcgg tttttaacag gccgattctg cccttaattc tgatgggcgt
     1561 gttagaaaaa aatgtggtac aacaactcag gcaacacgca aacgatttac tcgtcgtatt
     1621 tcaagttgca tgtgctgcgg ctacgttcgt tcaccccagt cacttactat tgtaagctcc
     1681 tggagttcac tcgcttgccg ccttcctgca actcgaaatc catagagtaa ataaacatca
     1741 cctttcagtg cgctattgca gccgcctgaa agaatagtgt atcgacctac atcacagacc
     1801 gcaggaaagg gtcaatataa tgattccaca gatttccctg gcacccggcg tcgttcaact
     1861 ggtgcttaat tttttgcaag agctggagca acaaggtttt accggcgatg cggcgacaag
     1921 ttatgccgat cgtctgacaa tgtcgaccga caacagtatt taccaacttc tccccgatgc
     1981 ggtggtattt ccgcgttcaa ccgcagatgt ggcgctgatc gcccgtcttg ccgcgcagga
     2041 acgctattca tcgctgatct ttaccccccg cggcggcggc accggcacta acggtcaggc
     2101 gctcaaccag gggattattg ttgatatgtc ccgccatatg aaccgcatca tcgaaattaa
     2161 ccctgaagag ggctgggtgc gcgttgaggc cggggtgata aaagaccaac tcaatcagta
     2221 cctgaaaccg ttcggctact tttttgcgcc ggaactttcg accagcaacc gggcaacgct
     2281 cggtgggatg atcaataccg atgcatccgg tcagggatcg ctggtctatg gcaaaacgtc
     2341 agatcacgta cttggcgtac gcgcggtgtt gttggggggc gatattctcg atacgcaacc
     2401 tttacccgtc gagctggcgg aaacgctggg taaatccaat accacaatcg ggcgaattta
     2461 taacacggtt tatcaacgtt gccgtcagca acgccagtta atcatcgaca acttccccaa
     2521 acttaaccgc tttcttaccg gttacgatct gcgtcatgtc tttaacgatg agatgaccga
     2581 gttcgacctg acgcgcattc tgacgggttc agaagggacg ctggccttta ttaccgaagc
     2641 gcggctggat attacgcgct tgcctaaagt gcgccgtctg gtgaatgtca aatatgactc
     2701 ttttgactct gcgctgcgta acgcgccgtt tatggttgag gcgcgggcgc tttcggtaga
     2761 gacggtggac tcaaaagtgc tgaatctggc gcgggaagat attgtctggc attccgtcag
     2821 cgagttgatt accgatatgc ctgacaaaga gatgctcgga ctgaacattg tggaatttgc
     2881 tggtgatgat gaggcgctga ttgacgagcg agttaatgca ctctgtgtgc ggcttgatga
     2941 gctgattgcc agccaacagg caggtgtgat tggctggcag gtgtgccgcg agctggcggg
     3001 ggttgaacgt atctatgcga tgcgcaaaaa agccgttggt ctgcttggca atgccaaagg
     3061 tgccgctaag ccaattccgt ttgctgagga tacctgcgta ccgccggaac atctggccga
     3121 ttatattgct gaatttcgcg cgctgctcga cagccacggc ttaagctacg gtatgttcgg
     3181 tcacgtcgac gcaggtgtct tgcacgtccg tccggcactg gatatgtgcg atccgcaaca
     3241 agagattttg atgaagcaaa tctctgatga cgtggtggcg ctgactgcga aatacggtgg
     3301 tttgttgtgg ggcgagcacg gcaaaggttt tcgcgctgaa tacagcccgg cgtttttcgg
     3361 tgaggaactt tttgcagaac tgcgcaaagt gaaagcggca tttgacccgc ataaccgact
     3421 caacccaggg aagatttgcc cgccagaagg tctcgatgcg ccgatgatga aagtggacgc
     3481 ggtgaagcgc ggtacattcg atcggcagat ccccattgcg gtacgccagc agtggcgcgg
     3541 tgcgatggag tgtaacggca acggtttatg cttcaacttt gatgcccgta gtccgatgtg
     3601 tccgtcgatg aagatcaccc agaaccggat tcattcaccg aaagggcgcg caacgctggt
     3661 gcgtgaatgg ctgcgcttgt tggcggatcg cggcgttgac ccactcaaac tggaacaaga
     3721 actgcctgaa tccggcgtca gtttgcggac gttaattgcc cgcacgcgca atagctggca
     3781 tgcgaataaa ggcgaatatg acttctcaca cgaagtcaaa gaggcgatgt cgggctgtct
     3841 ggcctgtaaa gcttgttcga cccagtgccc catcaaaatt gatgtgccgg agtttcgctc
     3901 tcgttttctg cagctctatc acacccgtta tttacgcccg cttcgcgacc acctcgttgc
     3961 tacggtcgag agctacgcac cgctgatggc acgcgcgccg aaaaccttta acttcttcat
     4021 taaccagccg ctggtgcgca aactctcgga aaaacatatc ggcatggttg atttgccact
     4081 gctgtcggtc ccctcgctac aacaacaaat ggtggggcat cgctcggcaa acatgacgct
     4141 ggaacagctt gaagctctca atgcagagca gaaagcgcgc acggtgttgg tggtgcagga
     4201 cccctttacc agctattacg atgcgcaagt ggtggcggat tttgtccgtc tggttgaaaa
     4261 attaggtttc cagcctgtgt tactgccatt ttcgccaaat ggcaaagccc agcatatcaa
     4321 aggctttctt aatcgttttg cgaagacggc gaaaaagacg gcggatttcc tcaaccgtat
     4381 ggctaagctt gggatgccaa tggtgggcgt cgatccggcg ctggtacttt gttatcgcga
     4441 tgaatataaa ctggcgctgg gcgaggaacg tggcgagttt aacgtcttac ttgcgaatga
     4501 atggctggca agcgcacttg agtcacatcc agtggctaca gtcagcggtg aatcatggta
     4561 tttctttggt cactgtaccg aggttaccgc tttgccgggc gcgccagcac aatgggccgc
     4621 gatatttgcc cgttttggcg cgaaactgga aaatgtcagc gtgggttgct gcggcatggc
     4681 agggacttac ggacatgaag cgaaaaacca tgaaaattcg ctcgggatct atgagttatc
     4741 ctggcatcag gctatgcagc gactgccgcg taaccgctgt ctggcgaccg gatattcctg
     4801 ccgtagccag gtaaaacggg ttgaaggcac gggggtacgc catcctgtgc aggctttact
     4861 ggagattatt aaatgatatg gaaacggaaa atcaccctgg aagcactgaa tgctatgggt
     4921 gaaggaaaca tggtggggtt cctggatatt cgctttgaac atattggtga tgacaccctt
     4981 gaagcgacaa tgccagtaga ctcgcggaca aagcagcctt tcgggttgct gcatggagga
     5041 gcatccgtgg tactggccga aagtatcggt tccgttgccg gttatttatg taccgaaggt
     5101 gagcaaaaag tggttggtct ggaaatcaat gctaatcacg tccgctcggc acgagaaggg
     5161 cgggtgcgcg gcatatgtaa accgttgcat ctcggttcgc gtcaccaggt ctggcagatt
     5221 gaaatcttcg atgagaaagg gcgtttgtgc tgttcgtcac gattgacgac cgccattttg
     5281 tgacgttgtc accagaaaag tgtgacgcat attgcttttg gtcattatct gcacagtaaa
     5341 cggtggtata ctgtgcaggt cttgtctaaa agcgaggatg ctatgtctac tcaacttgat
     5401 cccacccagc tggccattga atttttacgc cgtgaccaga gcaatctttc acctgcccaa
     5461 tatctcaaac gtctgaaaca actagaactt gaatttgctg acctgcttac tctctcttct
     5521 gctgaattaa aagaagaaat ctactttgcc tggcgtctgg gcgtgcattg atcctgactt
     5581 aatgccgcaa gttcgccaaa tcgacctgga ttaacctccg tccaggttcg atgaccttct
     5641 taatactgct tatctaccgc ctgatattgt tcaaaaataa tactaatcgc agtttgtgtt
     5701 aaaacggcgg gttagcttta tgagattatt cttatcgccc cttcaagagc taagccactg
     5761 agagtgccgg agataagcgc cggatggggt agaaaccctt aagcctgtgt cgcacagact
     5821 taagggtttt cttatttctg aacgctgcac ttttttacct atctcttaaa tagtcatttt
     5881 caatacaact ttttcttttt catcacagcc ttaatgctcc ttagataata accattatct
     5941 aacaatgaga tacctaattc ttagcgtgcc tgttaaccca cacatcaggg gctatgctta
     6001 ttaaaagcag gcaaaaagaa tgttttacgg taaagcccct gcgtttgctg ggttgaactg
     6061 ataatcatta tcactaacat gctgttatac gctgaaagcg atgaagtgag gtaaatagat
     6121 ggacatgcat tcaggaacct ttaacccaca ggatttcgcc tggcaaggct taacgctgac
     6181 acccgcagcg gcgatacaca tccgcgagct ggtggcaaag cagccaggta tggtcggcgt
     6241 gcgcttaggt gtgaagcaaa cgggctgcgc gggctttggc tatgtgctcg acagtgttag
     6301 cgagccggac aaagacgatc tgctgtttga acacgacggc gcgaagctgt ttgtcccgct
     6361 gcaatcgatg ccgtttattg atggcacgga agtcgatttc gttcgtgaag gacttaatca
     6421 gatattcaaa tttcacaacc ctaaagccca gaatgaatgt ggctgtggcg aaagctttag
     6481 ggtataggcg gtactatgtc tcgtaatact gaagcaactg acgatgtcaa aacctggacc
     6541 ggcggcccgc tgaactataa agaaggattc ttcacccagt tagccaccga tgagctggca
     6601 aaggggataa acgaagaggt ggtgcgcgca atttcggcga agcgtaatga gccggagtgg
     6661 atgctggagt ttcgtctaaa cgcctatcgc gcatggctgg agatggaaga accgcactgg
     6721 ttgaaagcgc actacgacaa gctgaattat caggattaca gctactactc agcaccatcg
     6781 tgcggtaatt gtgacgacac ttgcgcgtct gaacctggcg cggtgcagca aactggcgcg
     6841 aacgcctttt taagtaaaga ggtggaggcg gcgtttgagc agttgggcgt tcccgtgcgg
     6901 gaaggcaaag aggtggcggt ggatgccatt ttcgactctg tttcggttgc caccacttat
     6961 cgcgaaaaac tggcggagca gggaattatt ttctgttcct ttggtgaggc gatccacgat
     7021 cacccggaac tggtgcgtaa atatctcggc accgtggtgc cggggaatga caacttcttt
     7081 gcagcactta atgcggcggt agcctctgac ggtacgttta tttatgtgcc taaaggcgtg
     7141 cgctgcccga tggaactttc cacctatttt cgcattaacg cggaaaaaac cgggcagttt
     7201 gagcgcacaa ttctggtggc cgacgaagac agctacgtca gctacattga aggctgttcc
     7261 gctccggtgc gtgacagcta tcagttgcac gcggcagtgg tggaagtcat catccataaa
     7321 aacgccgagg tgaaatattc cacggtacaa aactggttcc ctggcgataa caacaccggc
     7381 ggtattctca acttcgtcac caagcgtgct ttgtgcgaag gcgaaaacag caaaatgtca
     7441 tggacgcaat cagaaaccgg gtcagcgatt acgtggaaat atcccagctg cattttgcgc
     7501 ggcgataact ccattggtga gttttactca gtggcgctga ccagcggtca tcagcaagcg
     7561 gataccggca ccaagatgat ccacatcggt aaaaacacca aatcgaccat tatctcgaaa
     7621 gggatctctg ccggacatag tcagaacagt tatcgcggct tagtgaaaat catgccgacg
     7681 gcaaccaatg cgcgcaattt cactcagtgc gactcaatgc tgattggcgc taattgtggg
     7741 gcgcatacct tcccgtatgt cgaatgtcgc aataacagcg cacaactgga gcacgaggca
     7801 acgacatcac gtattggtga agatcaactg ttttactgcc tgcaacgcgg gatcagcgaa
     7861 gaagacgcca tctcgatgat tgttaacggt ttctgcaaag acgtgttctc agaactgccg
     7921 ctggaatttg ccgttgaagc acaaaaactc ctcgccatca gtcttgaaca cagcgtcgga
     7981 taaggaataa acatgttaag tattaaagat ttacacgtca gcgtggaaga taaagctatc
     8041 ctgcgtggat taagcctcga cgttcgtccc ggcgaagttc acgccattat ggggccaaac
     8101 ggttcgggca aaagtacctt atcggcaacg cttgccgggc gcgaagatta tgaagtgacg
     8161 ggcggcacgg ttgagttcaa aggcaaagat ttgcttgcac tgtcgccgga agatcgtgca
     8221 ggcgaaggca tctttatggc cttccagtat ccggtggaga ttccaggcgt cagtaaccag
     8281 tttttcctgc aaacggcact taatgcagtg cgcagctatc gcggccagga aacgctcgac
     8341 cgctttgatt ttcaggattt gatggaagag aaaatcgctc tcctgaagat gccggaagat
     8401 ttattaaccc gttcggtaaa cgttggtttt tccggcggcg agaaaaagcg caacgatatt
     8461 ttgcaaatgg cggtgctgga accggagtta tgcattcttg atgagtcgga ctccgggctg
     8521 gatattgacg cattaaaagt ggtcgccgat ggcgtgaact cgctgcgtga tggcaagcgc
     8581 tcattcatca ttgttacgca ctaccaacgc attctcgact acatcaagcc tgattacgtt
     8641 catgtgctat atcagggacg aattgtgaaa tccggcgatt tcacgttggt caaacaactg
     8701 gaggagcagg gttatggctg gcttaccgaa cagcagtaat gcgctgcaac agtggcatca
     8761 cttgtttgaa gctgaaggga caaaacgctc accgcaagca cagcagcatt tacaacaatt
     8821 gctgcgtacc ggactgccga cacgtaaaca tgaaaactgg aaatatacgc cgctggaagg
     8881 gctgaccaat agtcagtttg tcagcattgc gggagatata tccccacagc agcgtgatgc
     8941 cttagcgtta acgttagacg ccgtgcggct ggtgtttgtc gatggacgtt acgtgcccgc
     9001 gctgagcgat gcaattgaag gcagcggata tgaagtgagc attaacgacg accgtcaggg
     9061 gttacccgac gctattcagg cggaagtgtt tctgcatttg acggaaagcc tggcacaaag
     9121 cgtgacgcat atcgccgtga agcgcggtca acggccggca aagccattgc tgttaatgca
     9181 tatcacccag ggcgtggcag gtgaagaggt gaacactgcc cattaccggc atcatctgga
     9241 tctggcggaa ggtgccgaag caacggtgat cgaacatttt gtcagcctta atgatgctcg
     9301 tcattttacc ggggcacggt tcactatcaa tgtcgcagcg aacgcccact tgcagcatat
     9361 caagctggcg tttgaaaacc cggtcagtca ccactttgcc cataacgatt tgttgctggc
     9421 tgacgatgcc accgcattta gccatagttt cctgctgggt ggcgcagtgt tacgacacaa
     9481 caccagtacg caactcaatg gcgaaaacag cacgctgcgg atcaatagcc tggcgatgcc
     9541 ggtgaaaaac gaggtgtgtg atacccgtac ctggctggaa cacaataaag gtttttgtaa
     9601 cagccgacag ttgcacaaaa ctatcgtcag cgacaaaggc cgcgcggtat ttaacggttt
     9661 gatcaacgtc gcgcagcacg ccatcaaaac ggatggtcag atgaccaaca ataatctgct
     9721 gatgggcaaa ctggcggaag tggatacaaa accgcagctg gaaatctatg cagatgatgt
     9781 gaaatgcagc cacggcgcga cggtggggcg tattgatgat gaacagatgt tctatctgcg
     9841 ctcgcgcggg atcaatcagc aggatgccca gcagatgatc atttacgcct ttgctgccga
     9901 actgacggaa gcactgcgtg atgaggggct taaacagcag gtgctggccc gaatcggtca
     9961 acggctgcca ggaggtgcaa gatgactttt tccgtcgaca aagtgcgggc cgactttccg
    10021 gtgctttcgc gtgaggtaaa cggtttgccg ctggcttatc tcgacagcgc cgccagtgca
    10081 cagaaaccga gccaggtgat tgacgccgag gccgagtttt atcgtcatgg ctacgcggcg
    10141 gtgcatcgcg gtattcatac cttaagcgcc caggcgaccg agaaaatgga gaacgtgcgc
    10201 aagcgggcat cgctgtttat taatgcccgt tcggcggaag agctggtgtt tgtccgcggc
    10261 acgacggaag ggatcaatct ggtcgccaat agctggggca acagcaatgt gcgggcgggc
    10321 gataacatca tcatcagtca gatggagcac cacgctaaca ttgtcccctg gcagatgctt
    10381 tgcgcacgcg ttggcgcaga gctgcgtgtg atccctctca accccgacgg tacgctgcaa
    10441 ctggagacgc tgcctacgct gtttgatgag aaaactcgcc tgctggcaat tactcatgtc
    10501 tccaacgtgc ttggcacaga aaatccactg gcggaaatga tcacgcttgc gcaccagcat
    10561 ggcgcaaaag tgctggtgga tggcgctcag gcggtgatgc atcatccggt ggatgttcag
    10621 gcgctggatt gcgactttta cgtgttctcc gggcataaac tgtatggccc caccggaatt
    10681 ggcattcttt atgtgaaaga agccttgttg caggagatgc cgccgtggga agggggcggt
    10741 tctatgatcg ccaccgtcag cctgagtgaa ggcactacct ggaccaaagc accatggcgg
    10801 tttgaagccg gtacacccaa taccgggggc atcattggtc ttggcgcggc gctggagtat
    10861 gtttcggcgc tggggcttaa tagcatagcc gagtatgaac agaatctgat gcattatgcg
    10921 ctatcacagc tggaatctgt accggatctc actctctatg gccaacaaaa caggcttggc
    10981 gttattgctt ttaatctcgg taaacaccac gcctatgatg ttggcagttt tctcgataat
    11041 tacggcattg ctgtgcgtac cggacatcac tgcgctatgc cattaatggc ctattacaac
    11101 gtccctgcga tgtgtcgggc gtcgctggcc atgtataaca cccatgaaga agtggagcgt
    11161 ctggtgaccg gcctgcaacg tattcaccgt ctgctgggat aacagggagg cactatggct
    11221 ttattgccgg ataaagaaaa attgctgcgt aattttttac gctgcgccaa ctgggaagag
    11281 aaatatctct acattattga gctgggccag cgtctgccag aattacgcgc cgaagacgga
    11341 agcccacaaa atagcattca gggctgccag agtcaggtgt ggattgtcat gcgccagaat
    11401 gcgcagggaa ttattgaatt gcagggcgac agcgatgcgg cgattgtgaa agggcttatt
    11461 gcggtcgtct ttattctcta cgatcagatg acgccgcagg atattgtcaa tttcgatgtg
    11521 cgtccgtggt ttgaaaaaat ggcgctcacc caacatctca ccccatctcg ttcacaaggt
    11581 ctggaagcga tgattcgcgc aattcgcgcc aaagccgctg cacttagcta aactaaaggg
    11641 acagctttca tccggttggc tcacattgcc ggatggtcaa tgtttacact gaccggcaca
    11701 ggagtgtttt ggtttcaggt gaacataagc accatccgac gtttcagcgt gagtctccgg
    11761 caaatacagg aggtttacaa tgaaacgcgc gtctttgctt acactcacgc ttatcggcgc
    11821 ttttagcgcc atccaggctg cctgggcggt tgattatccg ctaccaccaa ccggaagccg
    11881 actggttggg caaaatcaaa cgtatacggt gcaagaaggg gataaaaacc ttcaggctat
    11941 tgcccgacgt tttgatactg cagcaatgtt gatccttgaa gccaataaca ctatcgcccc
    12001 ggtgccaaaa cctggtacga cgataactat tccttcacaa ctgttattac ctgatgcacc
    12061 gcgtcagggg attatcgtta accttgcaga gctgcgcctt tattattatc cgccgggaga
    12121 aaatattgtg caggtttatc caataggtat tggattgcag gggctggaaa cgccggtgat
    12181 ggaaacgcgt gttgggcaga aaatccctaa cccaacctgg acgcctacgg caggcattcg
    12241 tcagcgttcg ctggagcgtg gcattaaatt accgccagtc gttcctgccg gaccaaataa
    12301 cccgctagga cgttacgcac tgcgcctcgc gcatggtaat ggcgaatacc tcattcatgg
    12361 caccagtgcg ccggacagcg ttggtttgcg cgtcagttca gggtgtattc gcatgaatgc
    12421 accggatatt aaagccttgt tctccagcgt gcggacggga acgccggtga aagtgatcaa
    12481 cgaaccggtg aaatattccg tggaacctaa cgggatgcgt tatgttgaag tacatcgacc
    12541 actatcagca gaagaacagc aaaacgttca gacaatgcca tacacactgc cagcaggctt
    12601 tacgcaattt aaagacaata aggctgtaga tcagaagtta gtcgataaag cgttgtatcg
    12661 tcgggcaggg tatccggttt cggtgagcag tggagcaact cccgcagcca gcaatgcgcc
    12721 ttcagtagag tcagcgcaga atggtgaacc agagcaaggg aatatgttac gcgcgacgca
    12781 gtagtggtaa atagcagaca aaaaaatggc gcacaatgtg cgccattttt cacttcacag
    12841 gtactattac ttgcggtatt tagtagccat gttgtccaga cgctggttag cacgagctgc
    12901 gtcatcttta gcagcctgaa cgtcggaacg cattgcgttc acgtcgttgc tcagctggtc
    12961 aactttagcg ttcagagtct gaacgtcaga agacagctga tcgattttag cgttgctgga
    13021 gcaacctgcc agcagagtag aacccaggat taccgcgccc agtaccagtt tagtagcttt
    13081 cattattaat accctctaga ttgagttaat ctccatgtag cgttacaagt attacacaaa
    13141 gttttttatg ttg
//
LOCUS       DAZXUK010000097        13129 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZXUK010000097 DAZXUK010000000
VERSION     DAZXUK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13129
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.097"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..124
                     /locus_tag="ACH3L8_002193"
     CDS             <1..124
                     /locus_tag="ACH3L8_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4085295.1"
                     /translation="SKLTDTTVSIPITASYYVYDTNKVKSGALEATALINVKYD"
     gene            complement(206..544)
                     /gene="rcnB"
                     /locus_tag="ACH3L8_002194"
     CDS             complement(206..544)
                     /gene="rcnB"
                     /locus_tag="ACH3L8_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFT4085296.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(763..1593)
                     /gene="rcnA"
                     /locus_tag="ACH3L8_002195"
                     /pseudo
     CDS             complement(763..1593)
                     /gene="rcnA"
                     /locus_tag="ACH3L8_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            1715..1987
                     /gene="rcnR"
                     /locus_tag="ACH3L8_002196"
     CDS             1715..1987
                     /gene="rcnR"
                     /locus_tag="ACH3L8_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFT4085297.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      2079..2175
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2210..2998
                     /gene="thiM"
                     /locus_tag="ACH3L8_002197"
     CDS             2210..2998
                     /gene="thiM"
                     /locus_tag="ACH3L8_002197"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFT4085298.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            2995..3795
                     /gene="thiD"
                     /locus_tag="ACH3L8_002198"
     CDS             2995..3795
                     /gene="thiD"
                     /locus_tag="ACH3L8_002198"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFT4085299.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            3860..4677
                     /locus_tag="ACH3L8_002199"
                     /pseudo
     CDS             3860..4677
                     /locus_tag="ACH3L8_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            4729..5475
                     /locus_tag="ACH3L8_002200"
     CDS             4729..5475
                     /locus_tag="ACH3L8_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFT4085300.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5449..6414)
                     /locus_tag="ACH3L8_002201"
     CDS             complement(5449..6414)
                     /locus_tag="ACH3L8_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFT4085301.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(6411..7414)
                     /locus_tag="ACH3L8_002202"
                     /pseudo
     CDS             complement(6411..7414)
                     /locus_tag="ACH3L8_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            7454..8506
                     /gene="fbaB"
                     /locus_tag="ACH3L8_002203"
     CDS             7454..8506
                     /gene="fbaB"
                     /locus_tag="ACH3L8_002203"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFT4085302.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            8814..9668
                     /gene="gatY"
                     /locus_tag="ACH3L8_002204"
     CDS             8814..9668
                     /gene="gatY"
                     /locus_tag="ACH3L8_002204"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFT4085303.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            9697..10959
                     /gene="gatZ"
                     /locus_tag="ACH3L8_002205"
     CDS             9697..10959
                     /gene="gatZ"
                     /locus_tag="ACH3L8_002205"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFT4085304.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGHALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            10969..11421
                     /gene="gatA"
                     /locus_tag="ACH3L8_002206"
     CDS             10969..11421
                     /gene="gatA"
                     /locus_tag="ACH3L8_002206"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFT4085305.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            11452..11736
                     /gene="gatB"
                     /locus_tag="ACH3L8_002207"
     CDS             11452..11736
                     /gene="gatB"
                     /locus_tag="ACH3L8_002207"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFT4085306.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            11740..13095
                     /locus_tag="ACH3L8_002208"
     CDS             11740..13095
                     /locus_tag="ACH3L8_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFT4085307.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         3231 a         3211 c         3434 g         3253 t
ORIGIN      
        1 gtcaaaactt acagatacaa cggtttcaat tccgataaca gccagttact acgtctacga
       61 tacaaacaaa gttaaatctg gcgcactgga ggcaaccgca ttaatcaacg tgaaatacga
      121 ctaataacaa gcaaaacgag caagtggcta ataataaaaa ataacaaggt gtctgcattt
      181 ccccctcatg atgagggggc tttttttagc gatgataaaa aatctcaccg tcgtaggctt
      241 taatgatttt accgtcggtg tcgctgatca acacgtacgc gccacccata taggtccagt
      301 gcgtcccggc atcaggcgcg ggcaggttac gtaactgcca ctgcttaatg ttgtactcat
      361 cggtgcggta catctctggg acggtatcac ctggtttgaa atgcttaaag tcagcaatga
      421 aactacttaa ctcatactta gaaattcccg aggtattggt cgaacccgcg gtggctggtg
      481 cggcccaggc ggcactggta accagcaaaa gcacacccag caacatctta tttttaatgg
      541 tcataaactc tccactttgg ggatctgtaa tatcagtcgc atatctttcc tgtcatacaa
      601 tataactgac aagcgatatt ttgttagtga tcccatgatt tcgtaaaaag tgtaaatgaa
      661 aggatgccct aaaagcatcc ttagcgagca gagagtcagc ggaaatagct gatttaagaa
      721 tcatcctggg ctgaagataa catgcaagcc taaaggattg agttatcgca ttatgcccat
      781 gaagccatgt acgcccatat acacaccgac taagccaatc aacagactgg aaaaataggg
      841 ggcgcgttta gcgagagtgt taaatccgct ccagcgtttt gcgacctgct gaacgctgat
      901 tgctgcgcca acgcctacgg tgacaagcgt taacgccagg ccaatgctga aactgacgac
      961 cagtgttgcg cccagtgtca gggctttcaa ctgaatgcat atcaacagca cggtaattgc
     1021 tgccgggcag gggataaagc caccggttaa gccaaataac aaaatttgcc agttgatgac
     1081 ctctctacca tcaaagcgtc gtttaatgtc attggcatgg gctcgtgcat gggcatcctg
     1141 atactcgcca tgttcgtgat ggtgatgatg tccttggtcg tggtgatgtt cgtgatcgtg
     1201 atgatggtga tgatgctcat aatcatgccc gtgcatattc tccagccagt tgcgttcgcc
     1261 gcgctaggta cgccagaaca tccagaacgc ggtgccaata atgatcactg cggaaatcag
     1321 ctggagccac ggttctgctg attgagcagt aaagcgctta ctgatcacca tcccgccaaa
     1381 ggcaatcaac cagaccaccg cggtatgcga aatggttgct gccagtccga gcatcaccgc
     1441 ctgtttaatg gtgcctttga tggcgatgat aaacgccgcc atcatcgttt ttgagtgccc
     1501 tggttccagg ccatgaagcg caccaagtaa gatggcgctg gggatgaaga accaggcgtt
     1561 tccttgctga agaagagttg taaattcggt catgataata attcttagta ttaattcggc
     1621 aatctgattc tactcccccc cagtacctga tactaccccc ccagtagatt aatagtgcta
     1681 tgatttttca tattcttgtt aaccaggtgt tgccatgtct catacaatcc gtgataaaca
     1741 gaaactgaaa gcgcgtgcca gtaagattca gggccaggtc gtggcgctca agaaaatgct
     1801 cgacgagccg cacgaatgcg ctgcagtttt acaacagatt gctgctatcc gtggagcggt
     1861 aaacggtctg atgcgggaag tgattaaagg tcatctgacg gaacacatcg ttcaccaggg
     1921 ggatgagcta aaacgtgaag aagatctgga tgtcgttctg aaggtgctgg attcatatat
     1981 caaataattt attaacgcga ttgtaaaact gccgtttttc ctcgtttaca acgggggcgc
     2041 tggacattac catcctcctc tgcgatttat catcgcaacc aaacgactcg gggtgccctt
     2101 ctgcgtgaag gctgagaaat acccgtatca cctgatctgg ataatgccag cgtagggaag
     2161 tcacggacca ccaggtcatt gcttcttcac gttatggcag gagcaaacta tgcaagtcga
     2221 cctgctgagt tcagcgcaat ctgcgcacgc gttacacctt tttcaccaac attcccctct
     2281 tgtgcactgc atgaccaatg atgtggtgca aacctttacc gccaatacct tgctggcgct
     2341 cggtgcatcg ccagcgatgg ttatcgaaac cgaagaggcc agtcagtttg cggctatcgc
     2401 cagtgccttg ttgattaacg ttggcacact gacgcagcca cgcgcacagg cgatgcgtgc
     2461 tgccgttgag caagcaaaaa gctctcaaac acgctggacg cttgatccag tagcggttgg
     2521 tgcgctcgat tatcgccgcc atttttgtca tgaactttta tcttttaaac cggcagcgat
     2581 acgtggtaat gcttcggaaa tcatggcatt agctggcatt gctaatggcg gacggggagt
     2641 ggataccact gacgccgcag ctaacgcgat acccgctgca caaacactgg cacgggaaac
     2701 tggcgcaatc gtcgtggtca ctggcgaggt ggattatgtt accgatggac atcgtatcat
     2761 tggtattcac ggtggcgatc cgttaatgac caaagtggta ggaactggct gtgcattatc
     2821 ggcggttgtc gctgcctgct gtgcgttacc aggcgatacg ctggaaaatg tcgcatctgc
     2881 ctgtcactgg atgaaacaag ccggagaacg cgcagtcgcc agaagcgagg ggccaggcag
     2941 ttttgttcca catttccttg atgcgctctg gcaattgacg caggaggtgc aggcatgaaa
     3001 cgaattaacg ctctgacgat tgccggtact gatccgagtg gtggtgcggg gattcaggcc
     3061 gatcttaaaa ccttctcggc acttggcgct tatggttgct cagttattac tgcactggtg
     3121 gcgcaaaata cccgtggcgt acagtcggtg tatcgcattg agcctgattt tgtcgccgcc
     3181 cagctcgatt cggtgttcag cgatgtgcga atcgatacca ctaaaatcgg tatgttggcg
     3241 gaaaccgata ttgttgaagc ggtggcagaa cggttgcaac gttatcagat ccaaaacgtg
     3301 gtactcgaca ccgttatgct ggcaaaaagc ggcgacccgc tgctttcacc ttcggcggtt
     3361 gctacgctgc gcagtcgatt attgccacag gtttcattaa taacgccaaa cttgcccgaa
     3421 gctgccgcct tgctcgacgc gccacacgcg cgcaccgaac aggaaatgct ggaacaaggg
     3481 cgatcgctgt tggcgatggg ctgtggcgca gtgctaatga aaggtggtca tctggatgat
     3541 gagcaaagcc cggactggct gtttacccgc gagggtgaac aacggtttac cgcaccgcgc
     3601 attatgacca aaaacaccca cggcactggt tgtacactct ctgcggcgtt ggctgcacta
     3661 cgcccgcgcc atacaaactg ggctgacacc gtacaggagg caaaaatctg gctttcatcg
     3721 gcgttagccc aggccgacac gctggaagtt ggtcacggta ttggtccggt tcaccacttc
     3781 cacgcctggt ggtgagcacc gtactatagc tgctttggtt attgtcagcc tttggcactg
     3841 acattcaggg atgaaaccaa tgcagctaag aattaccagt cgaaaaaaac tcaccgctct
     3901 attatgcgct cttgggctaa tttccatcgt tgcaatttat ccgcgtcaga cggtgaattt
     3961 tttctattcg acagcagttc agattacaga ctacatccac ttctacggtt atcgcccggt
     4021 taaatctttc gctattcgca ttcctgccag ttacaccatt cacggattag atgtttcgcg
     4081 ctggcaggag cgtatcgact ggcagcgggt ggcaaaaatg cgcgacaacg atatccgctt
     4141 acagtttgct tttattaagg ctacggaagg cgaaaagctg gtggacccct atttttcgcg
     4201 taactggcaa ttaagccgcg aaaatggcct gctgcgcggg gcgtatcatt atttctcccc
     4261 gtcggtatcc gcttcagttc aggcgcgatt atttctgcaa acggtggatt tctcacaagg
     4321 cgatttccct gccgtgctgg acgtagaaga acggggaaaa ttatcggcaa aagaattacg
     4381 caagcgggta agtcagtggc taaaaatggt cgaaaaacgc acgggaaaaa agccgattat
     4441 ttactcagga gccgtttttt atcacaccaa tctggcgggc tatttcaatg aatatccgtg
     4501 gtgggtggct cactattatc aacgtcgtcc ggacaatgac ggcatggcct ggcgcttctg
     4561 gcagcattcc gaccgtggac aggtagatgg catcaatggt ccggtggatt ttaatgtgtt
     4621 taatggcacg gggatgagtt gcaggcattc gttgatggga ttaaagaaac gccttaaata
     4681 agaagcagtg atatactccc accagactcc ctggcaggaa aaagacaaat ggaacaagcg
     4741 catacccagc tgattgccca actgaacgag cgtattttag cggcggataa cacgccgctt
     4801 tatattaagt ttgccgaaac ggtaaaaaat gccgtgcgca gcggggtgct ggagcatggc
     4861 aatattttgc ccggtgagcg tgatttaagt cagttaaccg gcgtttcgcg cattacggtg
     4921 cgcaaggcga tgcaggcgct ggaagaagag ggcgtggtga cgcgttcgcg cggttacggc
     4981 acgcagatca acaacatctt cgaatactcg ttgaaagaag cgagaggttt ttctcagcag
     5041 gtggtattgc gcgggaaaaa gcccgatacg ctatgggtta ataagcgtgt cgtgaaatgt
     5101 cccgaagaag ttgcgcagca gctggcggtc gaagcgggaa gtgatgtctt tttgcttaag
     5161 cgtattcgct atgtcgatga agaagcggta tcgattgagg aatcgtgggt tccggcgcat
     5221 ttaattcatg atgttgatgc catcggaatt tctctttatg actatttccg cagccagcac
     5281 atttacccac aacgtacgcg ttcccgcgtt agcgcccgga tgccggatgc cgagtttcag
     5341 tcacatattc agttagatag caaaataccg gtgctggtga tcaagcaagt ggcgcttgac
     5401 caacagcaac ggcctattga gtacagcatt agccactgtc gcagcgatct atacgttttt
     5461 gtgtgcgagg agtagttcct cgcgcgttgg cgcacaatca ccgccccgat gcccgacaac
     5521 ccacgacgcc actgcattgc ccagtaatac ggcatccgcc agtggcagac cagaggccag
     5581 cccggcaagt acgccacctg catgactgtc gcccgcccca atggtgtcta caacttgcgt
     5641 tggaaatgcc ggaatgcagc cagaagcgtc gttgctgaaa taccatgcgc cttctttatc
     5701 gagacgaacg atcaacggcg cggcaaattt ctcctgccat tgcttgccaa gtgttgttat
     5761 ctctgcggat aaagcaaaac gttcggcggc aatctcagcc tcttgacgat tgagtgacac
     5821 taaaggtcga caggccatga tccgcgccag taatgcgtcc gggatatcgc caatacgtgg
     5881 gcaaaaatcg ataaacggcg tcacgtcttg cagcttttcc agccattcca ctaacaattc
     5941 gccgcagggc gaggccagtt gataaccgga aaaatagagc aggttgccag gcgcaacggt
     6001 taatcgtgcc aaccattggc gattccattg attttccaca ccgctgaatg acataaaagt
     6061 gcgttcgcca tccggctcaa ccagtgccag acaccagccg ttatcgcttt cggcgttatc
     6121 gatcaggctg attaacccct ctttcgccat ccggttgcga ataatctccg cccacacgcc
     6181 ctgaccgagc ggcaaggcat tacccgcttc gatgccgagg cgctttaacg ccacggcaat
     6241 attcagtgcg cagccgccaa cgttaacgct ctgctgtttc agttcgatat cgcacccacg
     6301 ccaggggagg gcataagcgt cggcgatcac gtcaatgacc gccgcgccga cgaccatcac
     6361 cggctgacgc gtggttaatt caggcagcag tgtgtgtaat cgagcgccgc tcataccgcc
     6421 tcccgttgtt gacgatattt cgccagcgct gtggcatagc ggttgaaatc aagctgattt
     6481 accgcatcca gctccgcctt taacgcagga tcgacagcgt taatgccatg caacgcgccg
     6541 caaatcgccg tcgccatagc acctatggtg tctgtgtcgc cgccaagatt ggcgcacagg
     6601 acggcgcagc ggtttgggtc ggtttgtgcc agttcaacta gcgcaatggc gcacggaacg
     6661 gactcaatag tgctggtgcc tgcgccaacg acctggtaaa gctgttcgct ggcggattcg
     6721 gtgccgtcgg cattacgcac aattttcagc gcaatctcca gacgtgccgc cagtgaggcg
     6781 ctgaacgtgg tggtgcgttt ttgctgtgca tgtcgcgcaa ttgaaggcaa tgaatcaaca
     6841 atcgctgacc agcgttctcc gtcaatggca cgagaaatcg cccatgcgat gactaccgcg
     6901 cctgcaaccg ccagatcgga tttatgggtc gggctggacg ccagcgccac atcatcaata
     6961 aaggaatcaa catcacgcgc tggaagcaaa caacctaatg gcgagacgcg catcgctgcg
     7021 ccgttggtta cgccgttgtt ttccagttca gcaacgggtt taccgtcgcg aatggcgtta
     7081 agcgcaatct tcgaggtcgg acctagtacg tttttgttaa aggcgtcgaa acgcaacgcc
     7141 cagtcgagaa tattacgccc aatcagattc ggatcgatct tgccttcaca ttccagtaac
     7201 gcatccgcca gacacaggcc atcgaggtat cgtcggtgaa ttcggcgcgg ttaaaataac
     7261 aggccgcgtt attctccttt ggtccaggca gaaaacggtc aatccagcca aagtgtgctt
     7321 taacgcggct gcgtggccaa agctcggagg gcatccccat cgcatcccct aacgcctgcc
     7381 cataaagagc accgagaata cgttctgttt tcatttaact tccccttgaa tcggaattta
     7441 cgggagcata gtaatgacag atattgcgca gttgcttggc aaagacgccg acaacctttt
     7501 acagcaccgt tgtatgacta ttccttctga ccagctttat ctccccggac atgactacgt
     7561 agaccgcgtg atgattgaca ataatcgccc gccagcggtg ttacgtaata tgcagacgtt
     7621 gtacaacacc gggcgtctgg ctggcacagg atatctttct attctgccgg ttgaccaggg
     7681 cgttgagcac tctgccggag cttcatttgc tgctaacccg ctctactttg acccgaaaaa
     7741 cattgttgaa ctggcgatcg aagcgggctg taactgtgtg gcatcaactt acggcgtgtt
     7801 ggtgtcggta tcgcggcgct atgcgcatcg cattccattt ctcgtcaaac ttaatcacaa
     7861 cgagacgcta agttacccga atacctacga tcaaacgctg tatgccagcg tggagcaggc
     7921 cttcaacatg ggcgcggtgg cggttggtgc gactatctat tttggctcgg aagagtcacg
     7981 tcgccagatt gaagaaattt ctgcggcctt tgaacgcgcg cacgagctgg gcatggtgac
     8041 agtgctgtgg gcctatttgc gaaactccgc ctttaagaaa gatggcgttg attaccatgt
     8101 ttccgccgac ctgactggtc aggcgaacca tctggcggca accataggtg cagatatcgt
     8161 caaacaaaaa atggcggaaa ataacggcgg ctataaagcg attaattacg gttataccga
     8221 cgatcgcgtg tacagcaagt taaccagcga aaacccgatt gatctggtgc gttatcagtt
     8281 agctaactgc tatatgggcc gggctgggtt gataaactcc ggcggtgctg caggcggtga
     8341 aactgacctc agcgatgcag tgcgtactgc ggttatcaac aaacgcgcag gcggaatggg
     8401 gctgattctt ggacgtaagg cgttcaagaa atcgatggct gacggcgtga aactgattaa
     8461 tgccgtgcag gacgtttatc tcgatagcaa aattactatt gcctgatacg cctcatacga
     8521 acagcaccac cccgtaggac ggataagacg tttacactgc atccggcaac ctatgcctga
     8581 tgcgacgcgg aagcgtctta tcatgcctac atagcactgc cacgtatgtt tacaccgcat
     8641 ccggcataaa aacacgcgca ctttgctacg gcttccctat cgggaggccg tttttttgcc
     8701 tttcgctcct cgaacaattt tcatattgtt gtttttgcga tcattatctc gacatttaaa
     8761 agcaaataat ttcattatat tttgaaatcg aaagcaaacg acaagatatg aaaatgtacg
     8821 tggtatcgac aaagcagatg ctgaacaacg cacagcgcgg cggttatgcg gttccggcat
     8881 tcaatattca caatctcgaa acgatgcaag tggtggtgga aaccgctgcc aacctgcatg
     8941 cgccggtcat catcgccgga acgcctggca catttactca tgctggtaca gaaaatctgt
     9001 tggcgctggt cagcgcgatg gcgaagcaat atcaccatcc actggcaatt catctcgacc
     9061 atcacacgaa atttgacgat atcgctcaga aggttcgttc tggcgtgcgc tcagtcatga
     9121 ttgacgcctc gcatttgcct tttgcgcaaa atatttcacg ggtcaaagag gtggtggatt
     9181 tttgccatcg ctttgatgtc agcgtcgagg cggagctggg gcaacttggc ggccaggaag
     9241 atgatgtgca agtcaatgaa gtcgatgcgt tgtacaccaa ccccgcacag gcgcgtgaat
     9301 ttgccgaggc aaccgggatt gattccctgg cggtcgccat cggcacggct cacggtatgt
     9361 atgccagcgc accggtgctt gatttttctc gactggagaa cattcgccag tgggtgaact
     9421 taccgctggt gctgcatggc gcgtcaggat tatcgactaa ggatattcag caaaccatca
     9481 aactggggat atgcaaaatc aacgttgcaa cggagctgaa aaatgccttc ttgcagtcgt
     9541 taaaaaatta cctgaccgag caccccgaag cgaccgatcc ccgggattat ttgcagtcgg
     9601 ctaaatccgc aatgcgcgat gtggtgagca aagtgattgc cgattgtggc tgcgagggca
     9661 gggcataacg cacctgccat ttaacaagga aaaaacatga aaacgttaat tgcccggcat
     9721 aaagctggtg aacatatcgg catatgttca gtctgttctg cccatccgtt ggttatcgaa
     9781 gcggcgctgg catttgatcg caacagcacg cgcaaagtgc tgattgaagc aacgtcaaac
     9841 caggtcaatc aatttggcgg ttataccgga atgacaccgg cagactttcg cgaatttgtt
     9901 tttacgattg ccgataaagt tgggtttgca cgcgaacgca ttattctcgg cggcgatcat
     9961 ctgggaccaa actgctggca gcaagaaaat gcggatgcgg cgatggaaaa atccgtcgag
    10021 ctggtaaaag catatgttcg tgctggcttc agtaaaattc atcttgatgc gtcaatgtcc
    10081 tgcgcggggg ataccatacc gttagcacca gaaacggttg cggaacgagc tgctgtgctt
    10141 tgctttgctg cggaaagtgt ggcgacagat tgccagcgtg agcaactgag ctatgtcatt
    10201 ggcactgaag ttccggttcc gggcggtgag gccagcgcca ttcagtcagt acacatcacc
    10261 catgttgaag atgccgccaa tactttacgt acgcatcaaa aggcctttat tgcccgtggg
    10321 ctgacagagg cgttaacacg tgtgattgcc atcgtggtgc agccgggtgt ggaatttgat
    10381 cacagcaata ttatccatta tcagccgcag gaagcgcagg cgctggcgca atggatagaa
    10441 aacacccgaa tggtttatga agcacattct accgattacc agacccggac ggcttattgg
    10501 gaattagtcc gcgatcactt tgcaatattg aaagtcggtc acgcattaac ctttgcttta
    10561 cgcgaggcga tatttgcact ggcacaaatt gagcaggaac ttatcgcccc cgaaaatcgc
    10621 agcggttgcc tggcggtaat tgaagaagtg atgctcgacg aaccgcaata ctggaaaaaa
    10681 tattatcgca cgggttttaa cgattcatta ctggatattc gttacagcct gtcggatcgt
    10741 attcgttatt actggccgca tagtcggatt aaaaatagcg ttgaaacgat gatggtgaat
    10801 ctgcaaggcg tggacatccc actgggcatg attagtcagt atcttcccaa acaatttgaa
    10861 cgcattcagt ccggggaatt atcagcaatg ccgcatcagc ttattatgaa taaaatttat
    10921 gatgttttgc gcgcctatcg ctacggctgt gcggaataag gacggtatat gactaacctg
    10981 tttgttcgta gcggaatttc ttttgtcgat cgtagcgaag ttttaaccta tatcggtaat
    11041 gagatgctcg cgaaaggtgt ggttcatgat acctggccac aggcattaat tgccagagaa
    11101 gcagaattcc ctaccgggat aatgcttgag cagcacgcca ttgcaatacc gcattgtgag
    11161 gcgattcatg ctaagtcgtc agccatttat ctgttaaggc caacaaataa agttcatttt
    11221 cagcaagcgg atgatgataa cgacgtggcg gtatcgttgg ttattgcgtt gattgtggaa
    11281 aatccgcagc agcaattgaa acttttacgc tgtttatttg gcaagttaca acagcccgat
    11341 atcgtcgaga cactaattac tcttcctgaa actcagttaa aggaatactt cacaaagtat
    11401 gttttagatt cagacgaata aatccctctg taacaataaa aaggactatt tatgaaacgc
    11461 aagattattg tcgcttgcgg aggcgcggtt gcgacctcta cgatggcggc ggaagaaatt
    11521 aaagagttgt gtcagagtca taatattcct gttgaattaa tccagtgtcg ggttaatgaa
    11581 atagaaacct atatggatgg tgtgcatttg atatgtacca ctgccagggt ggatcgtagt
    11641 tttggcgata ttccgttagt tcacggcatg ccttttgttt ctggtgtcgg tatcgaagca
    11701 ttacaaaata aaattctgac tatcttacag ggatgaccta tgttttcaga agtcatgcgt
    11761 tatattctcg acctcggccc tacagtgatg ctgccggttg tcatcattat tttttctaaa
    11821 atattaggca tgaaggcagg cgattgcttt aaagcgggtc tgcatatcgg gattggcttt
    11881 gttggcattg gccttgtgat tggcttaatg ctggattcca ttggtccggc ggcgaaagcg
    11941 atggcggaaa atttcgacct gaatctgcat gtggtcgatg tcggctggcc gggctcttca
    12001 ccaatgacct gggcgtcgca aattgcgctg gtggcgattc cgattgcgat attggttaac
    12061 gtggcgatgt tactgacccg tatgacgcgg gtggtaaatg ttgatatctg gaatatctgg
    12121 catatgacct tcaccggcgc gttgctgcac ctggcaaccg gttcatggat gatagggatg
    12181 gcgggtgtgg taattcacgc ggcgtttgtt tataagctcg gcgactggtt tgcccgcgat
    12241 acccgaaatt tctttgagct ggaaggtatt gctattccgc acggtacgtc ggcgtatatg
    12301 gggccgattg cggtgctggt cgatgctatc atcgagaaaa tcccaggcgt taaccgaatt
    12361 aaatttagcg ccgacgatat tcagcgcaaa tttggccctt ttggcgaacc tgtcaccgtg
    12421 ggttttgtga tggggctgat tatcggcatc ctcgcgggtt acgatgtcaa aggtgtattg
    12481 cagctggcgg taaaaacggc ggcagttatg ctgctgatgc cacggatgat taaacccatc
    12541 atggatggtt taacgcccat cgctaagcag gcccgtagtc gtttacaggc gaagttcggc
    12601 ggtcaggagt tcctgattgg cctggatccc gcattactgc tggggcatac ggcggtggta
    12661 tcggcaagcc tgatttttat tccgctcacc attttaattg ctgtttgtgt tccgggtaat
    12721 caggtactgc cgtttggcga tcttgccact atcggcttct tcgtggcgat ggcggtcgcg
    12781 gtgcatcgtg gaaatctgtt ccgcacctta atctcgggtg tcatcattat gagcatcacc
    12841 ctgtggatcg cgacgcaaac tattggtttg cacacccaac cggcggctaa tgctggggcg
    12901 ttaaaagccg ggggtatggt ggcttcaatg gatcagggcg gttctcccat tacctggtta
    12961 ctgattcagg ttttctcccc gcaaaatatt cccggtttca ttattatcgg cgcaatttat
    13021 ctgaccggta ttttcatgac ctggcgtaga gcgcgtggtt ttactaaaca agagaaagcc
    13081 gttctcgcag aataattttt acctgagggg gaggttatcc cccttaaca
//
LOCUS       DAZXUK010000098        13119 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZXUK010000098 DAZXUK010000000
VERSION     DAZXUK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13119
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.098"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..222)
                     /locus_tag="ACH3L8_002209"
     CDS             complement(<1..222)
                     /locus_tag="ACH3L8_002209"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085308.1"
                     /translation="MPEQVITKAPYIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEIIK"
     gene            159..1658
                     /gene="pitB"
                     /locus_tag="ACH3L8_002210"
     CDS             159..1658
                     /gene="pitB"
                     /locus_tag="ACH3L8_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFT4085309.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1707..2399)
                     /locus_tag="ACH3L8_002211"
     CDS             complement(1707..2399)
                     /locus_tag="ACH3L8_002211"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFT4085310.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2573..3286
                     /locus_tag="ACH3L8_002212"
     CDS             2573..3286
                     /locus_tag="ACH3L8_002212"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFT4085311.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3318..4076
                     /locus_tag="ACH3L8_002213"
     CDS             3318..4076
                     /locus_tag="ACH3L8_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085312.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            4122..5471
                     /locus_tag="ACH3L8_002214"
     CDS             4122..5471
                     /locus_tag="ACH3L8_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFT4085313.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5471..6295
                     /locus_tag="ACH3L8_002215"
     CDS             5471..6295
                     /locus_tag="ACH3L8_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085314.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            6307..6867
                     /locus_tag="ACH3L8_002216"
     CDS             6307..6867
                     /locus_tag="ACH3L8_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFT4085315.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6898..7968
                     /gene="lptF"
                     /locus_tag="ACH3L8_002217"
     CDS             6898..7968
                     /gene="lptF"
                     /locus_tag="ACH3L8_002217"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFT4085316.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            7965..9044
                     /gene="lptG"
                     /locus_tag="ACH3L8_002218"
     CDS             7965..9044
                     /gene="lptG"
                     /locus_tag="ACH3L8_002218"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFT4085317.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9080..10251)
                     /locus_tag="ACH3L8_002219"
                     /pseudo
     CDS             complement(9080..10251)
                     /locus_tag="ACH3L8_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(10251..10499)
                     /locus_tag="ACH3L8_002220"
     CDS             complement(10251..10499)
                     /locus_tag="ACH3L8_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFT4085318.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10532..11446)
                     /locus_tag="ACH3L8_002221"
     CDS             complement(10532..11446)
                     /locus_tag="ACH3L8_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFT4085319.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11443..>13119)
                     /locus_tag="ACH3L8_002222"
     CDS             complement(11443..>13119)
                     /locus_tag="ACH3L8_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFT4085320.1"
                     /translation="DFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTRAEAG
                     AKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSWSAKL
                     QGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPVPNDI
                     AYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMGLVGF
                     LLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNEKDLA
                     ELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSFSDEA
                     SGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPVAERV
                     VGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLIIIRG
                     RNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHALAARI
                     QSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         3131 a         3227 c         3412 g         3349 t
ORIGIN      
        1 tttgattatt tctaaaggtt ttgaatgaat gcttattgtc tgatacacaa gaaataacac
       61 tctttttatc gttaaaaaat gatatttcac tttgcccatg ccgttaaagt cactgataat
      121 gcgtccgttc gtaaattcaa aatggcgtaa tctaatatat gctaaattta tttgttggcc
      181 ttgatatata tacggggctt ttgttattac ttgctctggc atttgtgttg ttctacgaag
      241 caatcaatgg ttttcatgac acggcgaatg cggtggcaac cgttatttat actcgtgcca
      301 tgcagccaca acttgctgtg gtgatggcgg cattttttaa cttttttggc gtgttattgg
      361 gcggacttag cgttgcctat gccattgtcc atatgttacc aatcgatttg ttgctgaata
      421 tggggtcaac tcacggtttg gcgatggtct tttccatgct gctggcggcg attatctgga
      481 acctgggaac gtggttcttt ggtttaccgg cctccagttc gcacaccttg attggtgcga
      541 ttatcggcat cggtttaacc aacgcgctgt taaccggctc atcggtgatg gatgcgttaa
      601 acctgcgtga agtaaccaaa attttctcct cgctgattgt ttcccctatc gtcggcctgg
      661 tcattgcggg tggtctgatt ttcctgctgc gccgtttctg gagtgggacg aaaaagcgtg
      721 accgtattca ccgcattccg gaagatcgca aaaagaaaaa gggcaaacgt aaaccgccat
      781 tctggacgcg tattgcgctg attgtttccg ctgcgggcgt ggcgttttcg cacggcgcga
      841 acgacgggca gaaagggatc ggcctggtaa tgctggtact ggtggggatt gcccctgctg
      901 gcttcgtcgt caatatgaat gcgtccggct atgaaattac ccgtacccgc gatgccgtta
      961 ccaacttcga acactacctg caacagcatc ctgaactgcc gcagaagttg attgcgatgg
     1021 aacctccatt gcctgcagca tcgactgatg gcacgcaagt aacagagttt cactgtcatc
     1081 cggcaaatac ctttgatgcg attgcgcgcg ttaaaacgat gctgccaggc aatatggaaa
     1141 gttacgagcc gttaagcgtg agtcagcgca gccagctgcg ccgcattatg ctgtgcatct
     1201 ccgatacctc cgcgaagctg gcgaaactgc caggcgtcag taaagaagac cagaacctgc
     1261 tgaaaaaact gcgcagcgat atgttaagca ccattgagta cgctccggtg tggatcatca
     1321 tggcggtagc actggcgctc ggcattggca ccatgattgg ctggcgtcgt gtagcgatga
     1381 ccatcggtga gaagattggt aagcgcggca tgacgtatgc gcaaggcatg gcggcacaaa
     1441 tgacggcggc agtgtctatc ggccttgcca gttatattgg gatgcctgtc tccacaacac
     1501 acgtcctctc gtctgcggta gcagggacga tggtggtgga cggcggtggg ttacagcgta
     1561 aaacggtaac cagcatcctg atggcgtggg tgtttacttt accggcggca atttttcttt
     1621 ctggtgggct gtactggata gcattgcagt tgatttaacc ttcctgaaaa tgcccggtcc
     1681 tggcgatcgg gcatttccat ttttgactag tgataaccac gcgcggtcat aaaatccgta
     1741 atcgcttttt ctgcatcaac caacacctgt tccagtggct gattggcatc gatatcaacc
     1801 agttgtgcac caccaaaggt taactgtggc gttatggcaa tcttcctcgc cagcgattcc
     1861 cgtttatggt cgggtttacg tgcacaggca acttcaaggt caacattgag tttgatgacc
     1921 agatcaggct tatggctcgc catccagtga aacgctttac gttcctggct tgccagccat
     1981 gagacaaaac gaccaccttc aacgttaggt gggaacaccg taccatcgta agcgccagga
     2041 atttggtcct gaggataacg gtcggttaga acaattaacc cgcgacgacg acaggcaagc
     2101 atatgacgaa agcgcagtaa gcgacgggcg acaaacgctg taattaccag cgccggaact
     2161 ggtccaggca atttttttgc tgttttcacc tgatttcgtt caattgtttt atgtaaggat
     2221 tttcccatca aaggtaattt tgtcactgca cgaccgacat ttccggcctg ttttcctaaa
     2281 tgaactcttt cggcagcacc atatttttcg acaacggtaa taagatgttc acacaccgtt
     2341 gacttgcctg aagcatcgct accaataacg gcaattaatg gaggtgttat tgactgcatg
     2401 aatatatcct ttaatttaaa tatccattaa aaatatttat ttggttaata tgtttttatg
     2461 aaagcgtaat tcaggtcaat gtcacaatta gtcattgtca caataaggtt gaacggatat
     2521 tctgagcacg gaccacgtaa tattcaatac attatttact gccgggtttt tcgtgaatca
     2581 acgtaatatg tcaataatta attccacgcc agtgcgtgtt attgccattg taggatgtga
     2641 tggttcaggt aaatcgaccc tcacggcaag cctggtaaat gaactggcag caagaatgcc
     2701 aacagaacac atttatctcg ggcaatcgtc cgggcgaatt ggcgaatgga tttcacagct
     2761 ccctgttatt ggcgcacctt gtgggtgtta tctgcgaagt aaagcggcac atgtacacga
     2821 aaagccctta actccgcctg gcaatattac tgcgctggtt atctatctgc tttcctgctg
     2881 gcgggcgtac aagtttcgca aaatgttgtg taaaagccag caaggctatc tgctcatcac
     2941 cgaccgctac ccgcaggttg aagtgccggg ttttcgcttt gatggcccgc aattggcaaa
     3001 aaccacgggc ggtaacggtt ggataaaaat gttaaggcag cgcgagttga agctgtacca
     3061 atggatggcg tcttatttac ccgtattgtt gattcgtctt ggcattgatg aacaaaccgc
     3121 gtttgcgcgt aaacctgacc accaactggc agcgttacag gaaaaaatcg ccgttacgcc
     3181 gcaactgaca ttcaatggcg ccaagatcct tgaactggat ggtcggcaac cagccgatga
     3241 aattatgcaa gcgtcactac gcgcaattca cgccgcgctt tcctgatcac caaaatggat
     3301 aggggatgat ttaacgaatg gatgcactac aaacacaaac tgttaatagt acaaccgcgc
     3361 cgcagcccaa ctacattccg gggctgattg cggtggtcgg gtgtgatggc accggtaaat
     3421 ccacgctgac caccgacctg gtgaaatcgc tgcaacaaca ctggcaaacc gagcgccgct
     3481 atctggggct gctctccggc gaagacggcg acaaaatcaa acgattgccg ttggttggcg
     3541 tctggctgga acggcgattg gcggccaaat cctcgaaaac ccaaagcatg aaaaccaaat
     3601 ctccggcgct atgggcggcg gtgattatgt actgcttctc gctgcgaaga atggcgaatc
     3661 tacgcaaggt tcagcgactg gcgcaaagtg gcgttctggt ggtcagcgat cgcttcccgc
     3721 aggctgaaat ttcgggcttt tattatgacg gaccggggat cggcgtcgaa cgtgcgaccg
     3781 ggaaaatcag catgtttctg gcgcagcgcg aacggagttt ataccaaaaa atggcgcaat
     3841 accgtcctga attaatcatt cgtttgggga ttgatattga tactgccatc tcccgcaagc
     3901 ctgaccatga gtatgccgag ctgcaggaca aaatcggcgt catgtcgacg attggctata
     3961 acggcacaaa aattcttgag attgattcca gagcgcccta cagcgaagtg ctggaacagg
     4021 tacagaaggc ggtttctctg gttgccatcg tttctgaccg ccgaagttta acttaaggct
     4081 ggcgggatat tttttactat caacgataag gtcaggctga attggcgggt tttaacatca
     4141 aacattggtt tgcagatggc gcgtttcgca ccattattcg caatagcgcc tggttaggct
     4201 ccagtaatgt cgagagcgcc ttgctggggc tgttggcgct ctcgtgtgcc ggtaaaggga
     4261 tgacgcccgc catgtttggt gtactggtga ttgtgcaatc gtacgccaag tcgatcagcg
     4321 attttattaa gtttcagaca tggcagctgg tggttcagta cggaacgcca gccttgacca
     4381 acaataatcc gcagcaattt cgcaatgtcg tctcattttc cttctcgctg gatatcgtca
     4441 gcggcgcggt ggcgattgtc ggcggcattg ccttactgcc attcctttcc cattcattag
     4501 gtctggatga ccaaagcttt tggctggcgg cgctgtattg cacgctcatt ccttcaatgg
     4561 cttcctccac gccgaccggc attctgcgtg cggtagatcg ctttgatttg attgctgtac
     4621 agcaggcgac gaaacctttt ctgcgcgcag cggggagcgt cgcagcctgg tattttgact
     4681 ttggttttgt gggttttgtt attgcctggt acgtgtcgaa tctggttggc ggcaccatgt
     4741 actggtggtt tgccgcgcgc gaattacgcc gtagaaatat ccataacgcc ttcaaattga
     4801 atctgtttga gtctgcccga cacattaaag gcgcgtggag ttttgtctgg tcaaccaaca
     4861 ttgcccactc catctggtcg gcgcgtaact cgtgcagcac agtgttggtg gggatcgtgt
     4921 taggacccgc tgccgccggg ttatttaaaa tcgccatgac attcttcgac gccgccggaa
     4981 cgccagcggg tttgctgggt aaaagttttt acccagaggt gatgcgttta gatccgcgca
     5041 ccaccagacc gtggttgtta ggtgtgaagt ccggtttact ggcgggcgga atcggtattc
     5101 tggtggcgct cgcggtgttg atcgtcggca agccgctcat ttcgctggtg tttggcgtta
     5161 agtatctcga agcgtatgac ctgattcagg tgatgctggg cgcaattgtg atttcgatgc
     5221 tgggcttccc gcaggagtca ttgctattga tggcgggaaa acagcgcgct tttctcgtgg
     5281 cgcaaaccat cgcctcaatc ggctacatcg tattgctgtt catgttctgt catctgtttg
     5341 gtgtgctggg agcagcgttt gcttacttcg gtgggcaatg tctggacgtg gcgctctcgt
     5401 taattccaac cttaaaggca ttttttcagc gccattcctt gctttataac gcagccggag
     5461 agaaatcctg atgatgagaa aatatttccc gcttgaggcg agcgagcgtc tatttgttgc
     5521 gattgaagaa gatgatgtcg ttgatgcaca ggttagtttg ccgccgacta ttgcactaag
     5581 ttgtaccacc gaaatcattc atgataatta cgcgttatgc ctgcaattct ggctcaatgg
     5641 cgttgatcgt caggaattac tgcgtttagt tcgcaaacaa gccaaaggcg atgagctgac
     5701 tgctgatgag cgcaaacaat ttaaatacat gcgagcgcgc tacaagcacc tgcgttttgc
     5761 gcaacggctg tatttaaaga agcatcaggc cggattttta ttcggcaaaa cgaccgtttt
     5821 tctggggcgt tttcaggacg gctttcgtaa cggtaagaaa aatattgtgt cgtattacgg
     5881 caacctgtta cggatttatt taagctctcc ggtctggtcg ttggtgaact attcattacg
     5941 ccatagccag ttagaaagcg taagcagttt tattgcctat cggcaaaagc aaatgcatac
     6001 cctgaaagag attattgcca aaccgcgact aaccggcagg gaatttcatg atgtgcgaaa
     6061 aatcatcagc cagcaggttt cttattacga caccttaaga tcgcttgatc ctgaaaataa
     6121 agaggcgtta cagatttccc gtttcctcgc cgccattaat ggcctgatgg gagataaaca
     6181 cgacgacatg gtggcggatg atatggaaaa ccgccagtcg tattatgcac cgttggcgct
     6241 ggatagcgat atttgtcagc gcttagagct gctgatttcg cgttttccgc tatagccgga
     6301 gcaatgatgt catctcgttt gattattgcg ttgattatca tgctcttagc cccgggtgtg
     6361 caggcacaca atttcgtcac tggcaaaacg gttacccctg tttatatcca ggaaggcggc
     6421 gagctgcttc tgaatagcga cgacgaaatt cactaccaaa agtggaacag cacacaatta
     6481 gcgggaaaag tccgcattat tcagtatatc gccgggcgta agtcggcgaa gaaaaagaac
     6541 tcactgttaa ttaaagcggt tgaagcggca aactttccgc aggatcgttt tcagcccacc
     6601 accattgtga acaccgatga tgccatcttt ggtactggct attttgttgt tggcaaaatc
     6661 gaaaaaaata aacgccgcta tccgtgggcg caattcgtta ttgacggcaa tggacaggga
     6721 cgcgttgcct ggcgcttgcc ggagcagagt tcaaccattc tggtgctgaa taaagccggg
     6781 caaattcagt gggcaaagga cggcagcctg acgccggaag aggtagatca tgtcattgcg
     6841 ttggcgcaga aactgatcaa tgaataaaaa actcaaagga ttgagggcgt tagtgagatg
     6901 aagctggttg aacactacat catgcgtggt acgcgccgtc tggtgctgat tatcgtcggt
     6961 tttctgatct ttattttcgc cagctactcc gcacagcgtt atctgaccga agcggcaaat
     7021 gggacattag ctcttgatgt tgtgctggat atcgttttct acaaagtact gattgcactg
     7081 gagatgttgt tacctgttgg tctgtatgtg tcagttggcg tgacgctagg gcagatgtat
     7141 accgactcgg aaattaccgc tatctctgcg gcggggggca gtccgggacg cttgtacaaa
     7201 gccgttcttt atctggcgat accgctaagt atttttgtca cccttctgtc gatgtatggt
     7261 cgtccgtggg cttatgcgca gatttatcaa ctggagcagc agtcgcagtc ggagctggat
     7321 gttcgccagt tgcgggcaaa gaaatttaac actaacgata acggacgaat gatcctttcg
     7381 cagacggttg atcaggataa taatcgcctg actgacgcgc tgatttatac ttctactgcc
     7441 aatcgaaccc gtattttccg cgccggttcg gttgatgtgg ttgacccatc acccgagaaa
     7501 ccgaccgtta tgttgcacaa cggcactgcc tatcttctcg atcatcaggg gcgtgacgac
     7561 aacgaacaga tctaccgtaa tctgcaatta catctgaatc cgctggatca aagcccgaac
     7621 gtcaaacgca aagcgaaatc ggtcacggag ctggcgcgct ccgcctttcc tgccgatcat
     7681 gccgaactgc aatggcgaca aagccgtggc ctgaccgcat tgttgatggc gctgctggcc
     7741 atttcattaa gtcgggtaaa accgcggcaa gggcgatttt caacgttatt gccactgacg
     7801 ttgctgttta ttgccatttt ttatggcggc gacgtctgcc gtacactggt ggctaacggc
     7861 gcgattcccc tcattcctgg tttgtggtta gtgcccggac tcatgctaat gggcctgctg
     7921 atactggtcg cacgtgactt ctctttgctg cagaaatttt cccgatgaat gttttcagtc
     7981 gctatttaat ccgtcatctc tttctcggtt ttgccgccgc tgcagggcta ttgctgccgc
     8041 tttttaccac cttcaacctg attaacgaac tggatggtgt cagcccgggc ggttatcgct
     8101 ggactcaggc ggtgctggtg gtgctaatga ccttaccgcg cacactggtc gaactttcgc
     8161 cgtttatcgc gttattgggt ggtattgttg gactggggca gttatcgaaa aacagtgagc
     8221 ttaccgccat tcgcagcacg gggttttcta tcttccgtat tgcactggtg gcgctggttg
     8281 ccgggatatt gtggactgtt tcgttaggcg cgatagatga gtgggtggcg tcgccattgc
     8341 agcaacaggc gttgcaaatc aaatcgaccg ccaccgcgtt gggggaggac gatgacatta
     8401 ccggcaatat gctgtgggcc aggcgtggta atgaatttgt gacggtgaaa tcgctgaacg
     8461 agcagggcca gccggtgggc gtggagatat ttcattatcg cgacgatctc tcgcttgaat
     8521 cctacattta tgcacgcagt gccaccattg aagacgacaa aacgtgggtc ctgcatggtg
     8581 tgaatcataa aaaatggctg aatggcaaag aaacgctgga aacattagat aatcttgcct
     8641 ggcaatcggc cttcaccagt atgaatcttg aagagttatc aatgccgggg aatacttttt
     8701 ctgtccgtca gcttaatcat tacatccatt atttgcagga aaccgggcaa cccagcagcg
     8761 aataccacct tgcactgtgg gaaaaactgg ggcaacccat cctgaccctg gcgatgattt
     8821 tgctggctgt gccgtttacc tttagtgccc cgcgttcgcc tgggatgggt agccgtctcg
     8881 ctgtaggcgt catcgtgggc ttactcacct ggatcagcta tcaaatcatg gttaatctgg
     8941 gattgctatt tgcgttaagc gcacctgtta ccgcgctcgg tttaccggta gcgtttgtgt
     9001 tggtggcgtt gagcctggtg tattggtatg acagacaaca ttaaacccgc cgtggcgcaa
     9061 acaccacggc gggctcacat tactgcttca gagtagcaaa tgcctgaatg atggcatcga
     9121 tttgctcacg actgtgggca gcatttacgc tacagcgcag cagcgtgatg cctgcaggcg
     9181 cggcaggcgg caggataaga ttcacataca cgcccggcga gataagctcg cgccagatgc
     9241 gtaaaccttc ctctttcgaa ccgattatta ctggcactac cgggctaata tgtgaactta
     9301 actcataacc cagtttttgc agaccgtcat acagatggtt ggcgttatcc atcagctttt
     9361 gccgcagctc tggttgtgtg gcaatagttc ttaacgacga gcgcacggtc gcaatgcagg
     9421 acggagaagg agaggcggta aaaatatacg ggcggctgcc gtagcgcagc acttccatcg
     9481 cttccgaaac gacagcaaaa ccgccaatcg aggccagact tttactgaaa gtaccaacga
     9541 taatatccac atcatcttca acgccgacag cttcagccag tccgcgcccc gttgcgccta
     9601 acacgccaaa cgaatgcgct tcatcgacaa tcaaataacc accgagacga cgtttgatat
     9661 caacaatttc cgccagcggc gcaacgtcac cgagcatgct gtagatgccc tcaacgatga
     9721 taatcgcctc tttcgcccgt tcaccgaggc gaaccatgcg ccgttccaga tctttagcat
     9781 cgttatggcg gaaacgaata atttccgcgc caccgagaga acagacgtca taaatactgg
     9841 cgtggctgtc ggcatccagt aacaccacgg cgttatggtc ggcaagtgcg ctgatgacgc
     9901 ctaaattagc ggtataaccg gttgaaaaac gatagcggta gggcgattga aaaactcggc
     9961 aatctctttc tctaacgcca ggtgcggcgc ataactgccg tttgccatac gagaaccggt
    10021 ggtgccggtg ccttgggccg ctaacgcggc ctgaccttcg ctgatggcat catgattaaa
    10081 ggttaaaccg agataattat tggtcccggc cagaatcact tttttattgc cgatccggcc
    10141 ttcggtggcg gaatacactt catcaataca ggtgccaaac ggtgttaacc cgttgtcact
    10201 aaattgcaga cgctcgccag caaggcgcgc atatttatcg tatagcccca ttatttattc
    10261 tccagccatg gaagtagagt ttccattaat tgcgcagggg ttttgacatc cagcagaata
    10321 ttaataggaa tggaaatatc gaatctgtct tccagcatca ttaagagatc catcactttg
    10381 atcgactcca ggccaagatc gttgacgaga tcgctgtcgg gcttaatttc cacaccgttt
    10441 tcgaccaaat cctgtaaaca ggaaaggatg taatccatta ctatttcacg atttaccata
    10501 gaaacgttaa cacaccttta aaacaagaca atcaaaatag cccgttgcgt aatgcgtgtt
    10561 ccaggctaat cccgggaaac caattgatat cttcagaaat acggttatta cttgccgacc
    10621 agtcggcgtg ggttaattca cgaattttcg agcgggtcag cataggttct ttacctgcca
    10681 ggcgactcaa cgcggtactg atatccgcaa ggcctgtgag taccggcaga ggaatgtcaa
    10741 ccattcgcac ggaaccacaa cgggctttgg cggcaagctg tcgtacgcgt tgccagtcat
    10801 agccaccggc gacgccatcg cataattcat aggtttgcgt ctgtacagtt tcggcgctta
    10861 accactgacc cactgcttgc gcaaaatcgg tgacgtgaag aaaagagagt tgtgtatctg
    10921 gcgcaccaag tcgtggcagc aggccgcgta gcatccagtc aaacagcggt tttaactctt
    10981 tatcgccggg accatacacg gctgtcgggc gaaaaacgcc cagcgtaatt tcgtccgcca
    11041 ttgctgccag tcgttgctcg gcgacgtatt tggattttgc ataccaggag agctcgggat
    11101 ggcgcgccgc cagcgaagag ataaacagaa aacgttggca aaagccgctc tcttttgctg
    11161 cttgcatcag gcgcaggctg ccgtcaacgt tacagcgggt gaaaatctct tctttgtgcc
    11221 cgcgcacttg cccggcgcaa tggaccacta cgctggctcc ggcaaccagc tcgctaagtg
    11281 aatgagtatc ttccagcgaa ccgcgcaccc aggtgagatt atcattgacg tgagcgcggg
    11341 cagtacgcgt caatgcgcga acgtgaaagc cgcgggcgag caggttatcg ataatatatt
    11401 taccgataaa cccggtagcg cccgtcaccg cgacagtttg atttatgcca gggattcctg
    11461 cacattaaga ctggcagcat aagccttctg ataacgtttt ttcgcttccg cacgggcagg
    11521 cttgccggag gacgttcggg gaatactgtg gggcggcaac agatcgatag ccgcggtcac
    11581 gccaaattcg ctttggatcc gtgccgccag cgcgtggata agctgcccgc gacgttcttc
    11641 gtcgctgatc cgacactgga tctgcaaaat gattttttcc tgggcggtaa caaaagcaat
    11701 cgcatcgcca gaatgaattt ccggttcctg ttccgctata tattcaatat cctgcggcca
    11761 gatattacgg ccacgaataa taatcagatc tttaatgcgt ccggtgacat acagataacc
    11821 gtccagcaga taaccgaggt cgccggtgtc taaccagccc gtcgcggcaa tctcgtcttg
    11881 cgaaacctgg tcgccaaagt aaccgctcat cagactggga ccggagatgc aaatatggcc
    11941 taccacacgt tccgcgaccg gcatacctgc ttcattgcgg atttcaatac catgttccgg
    12001 caacgctttg ccgcagttga cgaaagtcga tacggcgcgt gtctctgcac ccggcgcgac
    12061 ggctttaccc tgatattcga ggatgtcgcg atccacttcg ttaaccacaa ccccggaggc
    12121 ttcatcagag aagctgacag ccagcgcatt ttctgccagt ccgtagcacg gcatgaaagt
    12181 tttattgtca aagttaacct gacggaaaca ttcagcgaat tgatggagtt gttctgcgga
    12241 gatcggctca gcaccaatac cagcgacgcg ccagcaggaa agatccagtt cagcgagatc
    12301 tttttcattc acgcggcgtt ggcacaattc atagccaaac ggcggcgcaa cggaaacggt
    12361 gccgcgattt ttactgatca atttaagcca ttgcaaagga cgcatggcaa aatcctgagt
    12421 gcgcaaataa tctactgaaa gttgcgtggc gacggggggc aggagaaagc cgaccagtcc
    12481 catatcatgg tagaaaggct gccaggagac gcagcggtcg ctagggcgta atttaatgcc
    12541 atcgtggctt atagcacgta gattagccat cacttcgcga tgggtgataa tgacgccacg
    12601 gggaaaacgg gtgctgccgg aggtgtactg gaggtaggcg atatcgttcg gaactggacg
    12661 ctggagcgca acatcggctt ccggtaatgc cttaaaccaa gcgtggctta aaacatgtaa
    12721 ttcggggttg ttatgcgtcg cggcattgac cagtggcaac cactcatcac cagtgataat
    12781 ggctgcgggc tggcaacttg ccagtaaacc ctggagtttg gcgctccagg aatcccgctg
    12841 accgacgccc attggaatcg ccaacgggac ggcgactaag ccagcatact gacaggcaaa
    12901 aaaagcctct acaaacccgc tactcgtttc ggcaatcagc gccacgcgtt cgcctttttt
    12961 caggttcagc gataacaacc gcttcgcacc agcttcggca cgcgttttta atgtctgata
    13021 ttccagttga tcttcaagtt ggcaacgtct gtcataaaaa ttcattccgg ctctactcag
    13081 agcggcgtag tccaaagcat caaccagcgt tggaaaatc
//
LOCUS       DAZXUK010000099        13084 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZXUK010000099 DAZXUK010000000
VERSION     DAZXUK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.099"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACH3L8_002223"
     CDS             complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACH3L8_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFT4085321.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="ACH3L8_002224"
     CDS             1131..1931
                     /locus_tag="ACH3L8_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFT4085322.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="ACH3L8_002225"
     CDS             2009..2779
                     /locus_tag="ACH3L8_002225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFT4085323.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACH3L8_002226"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACH3L8_002226"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFT4085324.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACH3L8_002227"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACH3L8_002227"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFT4085325.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="ACH3L8_002228"
     CDS             5034..5756
                     /locus_tag="ACH3L8_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFT4085326.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACH3L8_002229"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACH3L8_002229"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFT4085327.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACH3L8_002230"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACH3L8_002230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFT4085328.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="ACH3L8_002231"
     tRNA            7149..7225
                     /locus_tag="ACH3L8_002231"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="ACH3L8_002232"
     CDS             complement(7356..8402)
                     /locus_tag="ACH3L8_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFT4085329.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACH3L8_002233"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACH3L8_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACH3L8_002234"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACH3L8_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFT4085330.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACH3L8_002235"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACH3L8_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFT4085331.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="ACH3L8_002236"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="ACH3L8_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
     gene            complement(12179..13084)
                     /gene="tagK"
                     /locus_tag="ACH3L8_002237"
                     /pseudo
     CDS             complement(12179..13084)
                     /gene="tagK"
                     /locus_tag="ACH3L8_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137593242.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3006 a         3392 c         3480 g         3206 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatt
//
LOCUS       DAZXUK010000100        13007 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZXUK010000100 DAZXUK010000000
VERSION     DAZXUK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13007
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.100"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..309)
                     /locus_tag="ACH3L8_002238"
     CDS             complement(<1..309)
                     /locus_tag="ACH3L8_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFT4085332.1"
                     /translation="MAISDTKLRTIYGKPYSGPQELADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     Y"
     gene            complement(515..1234)
                     /locus_tag="ACH3L8_002239"
                     /pseudo
     CDS             complement(515..1234)
                     /locus_tag="ACH3L8_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1250..1582)
                     /gene="cusF"
                     /locus_tag="ACH3L8_002240"
     CDS             complement(1250..1582)
                     /gene="cusF"
                     /locus_tag="ACH3L8_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFT4085333.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1606..2988)
                     /gene="cusC"
                     /locus_tag="ACH3L8_002241"
     CDS             complement(1606..2988)
                     /gene="cusC"
                     /locus_tag="ACH3L8_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFT4085334.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3145..3828
                     /gene="cusR"
                     /locus_tag="ACH3L8_002242"
     CDS             3145..3828
                     /gene="cusR"
                     /locus_tag="ACH3L8_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFT4085335.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3818..5260
                     /gene="cusS"
                     /locus_tag="ACH3L8_002243"
     CDS             3818..5260
                     /gene="cusS"
                     /locus_tag="ACH3L8_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFT4085336.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5410..7647
                     /gene="nrfB"
                     /locus_tag="ACH3L8_002244"
     CDS             5410..7647
                     /gene="nrfB"
                     /locus_tag="ACH3L8_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFT4085337.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7634..10602
                     /gene="nfrA"
                     /locus_tag="ACH3L8_002245"
                     /pseudo
     CDS             7634..10602
                     /gene="nfrA"
                     /locus_tag="ACH3L8_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10603..11493
                     /locus_tag="ACH3L8_002246"
     CDS             10603..11493
                     /locus_tag="ACH3L8_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFT4085338.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11676..12437
                     /gene="envY"
                     /locus_tag="ACH3L8_002247"
     CDS             11676..12437
                     /gene="envY"
                     /locus_tag="ACH3L8_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFT4085339.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12483..12559)
                     /locus_tag="ACH3L8_002248"
     tRNA            complement(12483..12559)
                     /locus_tag="ACH3L8_002248"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12523..12525),aa:Arg,seq:tct)
     gene            12802..>13007
                     /locus_tag="ACH3L8_002249"
     CDS             12802..>13007
                     /locus_tag="ACH3L8_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFT4085340.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3184 a         3372 c         3303 g         3148 t
ORIGIN      
        1 gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa aataagtgcg
       61 tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc tggcatcctt
      121 caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat gccagcgata
      181 gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc catcagcatc
      241 agccaattct tgtgggccag aatatggttt accataaata gtacgcagtt ttgtgtcgct
      301 tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac atctggtaca
      361 caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg tagacatact
      421 caggtgaaaa aaaggttgtt tttcgagtta taacagacag ttaattaact acagtggaca
      481 atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg accacgttat
      541 ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca tcaatgggcg
      601 ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga atatccgcct
      661 ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc gccgtaccgc
      721 cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga atggtcaggt
      781 cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac accttgtcga
      841 taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa ctgacattcg
      901 tcgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt ttcaccccca
      961 gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct tcatcggcat
     1021 atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg aaccgcgtat
     1081 tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc attttttctg
     1141 cgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata ccgcccgcga
     1201 tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt tactggctga
     1261 ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac gccactttgt
     1321 cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc atggtcatct
     1381 ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg ctttccagat
     1441 caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg ctcatggttt
     1501 catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga ctgaacattg
     1561 cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt gctgccaacc
     1621 gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat tcaggtcaag
     1681 taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca gataacttac
     1741 cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg acgccagata
     1801 acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg catctgccac
     1861 ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct gctggcgaat
     1921 ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga tctcaatttt
     1981 aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat cgctgctggc
     2041 ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg cagcaccaat
     2101 attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc gctgcaataa
     2161 gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc tgtttactgt
     2221 ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat tattcgcctg
     2281 cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca ccccgcgagc
     2341 ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga caaacgcata
     2401 tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat acgccagctg
     2461 ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg cgcgctgggc
     2521 ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta agcgaccgaa
     2581 aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg tggctgaatc
     2641 gcctttaaga ttgccgctcc agctgccgct gccctcgcca ttgagctgtg ggtagcggtc
     2701 ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg ccatgcgcaa
     2761 atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat caacaaaaaa
     2821 ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt tctggctgag
     2881 tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca gtgaacaacc
     2941 agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag gctcataatt
     3001 tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg tgacaggaaa
     3061 atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta ctctagaatt
     3121 gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga aaagaaaacc
     3181 ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt ggccgacaac
     3241 gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat cctcgatatt
     3301 atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc caataaaggg
     3361 atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa ggggctggag
     3421 ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct ggcgcgggtg
     3481 cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca ggttgccgat
     3541 ctgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat cactctgacc
     3601 agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt gctgccccgc
     3661 tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa cgccattgat
     3721 gtggcggtga agctgctacg cggcaaaatc gacaacgact ttgagccgaa gctgattcaa
     3781 accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc catttcagcg
     3841 cccgttttcg ctggcaaccc gcctgacctt ttttatcagc caggccacca tcgcggcgtt
     3901 tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg agcaggacat
     3961 taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc ctgacgaaac
     4021 gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca acgtgttgat
     4081 ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc ctgatatccg
     4141 cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg tgtatctcct
     4201 ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac acagcaactg
     4261 gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt atacgctcta
     4321 catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga ataaacttat
     4381 tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg cggtacataa
     4441 aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct cgaaagatct
     4501 cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg tactgtcgtt
     4561 caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt tctcagcgga
     4621 tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg aaatcgccct
     4681 cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc tcgaagagct
     4741 gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg ataacaacca
     4801 gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag tgttcgattt
     4861 tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg acaaacgtca
     4921 ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt ctaatgccct
     4981 tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg atcaccaggt
     5041 gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac cgcgattgtt
     5101 tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta gcggtattgg
     5161 gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg taacgtcaga
     5221 tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca aacggcaact
     5281 tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg gtaaaaagct
     5341 gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc gttaatgtaa
     5401 ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg cttaaaagta
     5461 atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt ttttattgat
     5521 gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg ctacccgcga
     5581 atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat ggttccggcg
     5641 tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac gctcgactac
     5701 gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca acgtgatgtt
     5761 gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg tcctggtccc
     5821 accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt tgaacgtagc
     5881 gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat ttcaccgatg
     5941 gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat cccagtgtat
     6001 ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga gttttcagaa
     6061 ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc cagcgcaggc
     6121 gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg taacggtatt
     6181 gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct gaaagaaaaa
     6241 ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga acgcgagcag
     6301 cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga atatttcccc
     6361 gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat tgttttccag
     6421 ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct ctggcgcgac
     6481 cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat gatccagctt
     6541 ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct ggcatttcct ttctattttt
     6601 agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat ggttaaccgc
     6661 atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg cctgctttcc
     6721 gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg cgtgctgaaa
     6781 caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac gcatgacttc
     6841 cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct gctggaaaat
     6901 caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga aggtctacgc
     6961 ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc acaggcgctg
     7021 gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc gtcctcgctg
     7081 attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct gcgtctggaa
     7141 aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct ggcggccctg
     7201 acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca aattgtcaca
     7261 gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat gttgtacaat
     7321 gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg gcaatatgtg
     7381 ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa tcgttcagca
     7441 attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa gtttttggtc
     7501 accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca acgcgaatta
     7561 caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca ggttgcgcaa
     7621 ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt ctggtttact
     7681 gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca gcgcagaaga
     7741 gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata aggcgctgaa
     7801 ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata tacaccaaca
     7861 ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc attttggtca
     7921 tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag gagatgcccg
     7981 acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga ctgttgaaga
     8041 actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc gcagtgaagt
     8101 cgggcagaat gccctgcggc tggcacaatt acctgtcgcc agagcgcaac tgaacgatgc
     8161 gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc aacgggcaat
     8221 ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc agcagaacac
     8281 attaagcgcg gcagaacgcc gtcagtggtt tgacgtgctt cttgccgggc agctggacga
     8341 tcggatcctg gcactgcaat cacaggggat cttcaccgat cctcagtcat atattactta
     8401 cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc tcattgaaaa
     8461 taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt tatctaaata
     8521 cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg ataaccgcca
     8581 gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg acgcagcgca
     8641 aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg ctgtcagcgt
     8701 ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc agcaagaacc
     8761 gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg agcagtcacg
     8821 cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc gtgtcagcca
     8881 gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag caacgccggc
     8941 gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt ggcaaagtca
     9001 gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg atatgtcgcc
     9061 ttcctacgat gctgccgctt ggaaccgtct ggcaaagtgt tatcgggaca cgctacccgg
     9121 tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct ggcaacatcg
     9181 tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg cggcctggca
     9241 gaaaatcagt cttcacgaca tgagcaatga tgatctgctc gccgccgcca acaccgctca
     9301 ggcagcggga aatggcgcag ctcgcgatcg ctggcttcaa caggcagaac aacgtgagct
     9361 gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag ctcagccaga
     9421 actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca actcttacgt
     9481 tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga gtgatttgcg
     9541 cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg gttacgcctt
     9601 gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc ataaagggct
     9661 tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc tggatgacat
     9721 gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc aggcataacc
     9781 ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca tgaggaggtc
     9841 ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg cgcaatgagt
     9901 accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag ctacggacaa
     9961 ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct gctctcagtt
    10021 tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt gaaaaatccg
    10081 atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt cctcgccgtc
    10141 gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct gcgcgccagc
    10201 gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg ttcaggctgg
    10261 tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat tcaggcgtgg
    10321 acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac tattgagcct
    10381 tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc gcagcttggc
    10441 ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc ctggccgcac
    10501 aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca acgtaacgga
    10561 gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa gttcattttc
    10621 gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg tattatctgg
    10681 caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct gatgagtcag
    10741 ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg cgatgcattt
    10801 acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca acagactggc
    10861 ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca gaaacagtcg
    10921 tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca gcaagccaga
    10981 ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc ggaaattgac
    11041 gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt aaacaacgcg
    11101 cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt tttcgccgga
    11161 aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac cggagttaat
    11221 gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg tgaacgttat
    11281 ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt ttatgaactg
    11341 tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc agaaattgcc
    11401 tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc tctgcgctat
    11461 ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt cgggtacgac
    11521 ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta aaaagcatgc
    11581 taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa gcatagatcc
    11641 tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc agtgaacctt
    11701 gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat gctacgttta
    11761 cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt gaactctcaa
    11821 cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat tatctgttgt
    11881 ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctagcaacc ccggttatcg
    11941 cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc aagcagcaat
    12001 ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta ttgtctaact
    12061 ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc agcgtccgcg
    12121 acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg cgaattgtcg
    12181 ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac gaaaatacca
    12241 gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg ttattgatgg
    12301 ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg tcgtacttta
    12361 tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc aaacagcgac
    12421 aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa atgaagaaga
    12481 aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc gttgctctat
    12541 ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga attatacggt
    12601 caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac gaccgcgtct
    12661 gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg cgtctgaatc
    12721 aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg aaaattgtcc
    12781 gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga tactcatcct
    12841 atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca gattgtcctg
    12901 aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt tgatttaatc
    12961 attatggata tagacttgcc cggaacagac ggttttacct tcctgaa
//
LOCUS       DAZXUK010000101        12922 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZXUK010000101 DAZXUK010000000
VERSION     DAZXUK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12922
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.101"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            34..1044
                     /gene="glpX"
                     /locus_tag="ACH3L8_002250"
     CDS             34..1044
                     /gene="glpX"
                     /locus_tag="ACH3L8_002250"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFT4085341.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            1141..1887
                     /gene="fpr"
                     /locus_tag="ACH3L8_002251"
     CDS             1141..1887
                     /gene="fpr"
                     /locus_tag="ACH3L8_002251"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFT4085342.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(1892..2320)
                     /gene="uspD"
                     /locus_tag="ACH3L8_002252"
     CDS             complement(1892..2320)
                     /gene="uspD"
                     /locus_tag="ACH3L8_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFT4085343.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(2347..2646)
                     /locus_tag="ACH3L8_002253"
     CDS             complement(2347..2646)
                     /locus_tag="ACH3L8_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFT4085344.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(2858..3298)
                     /locus_tag="ACH3L8_002254"
                     /pseudo
     CDS             complement(2858..3298)
                     /locus_tag="ACH3L8_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            3399..3998
                     /locus_tag="ACH3L8_002255"
     CDS             3399..3998
                     /locus_tag="ACH3L8_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFT4085345.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKIQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            4106..4873
                     /gene="tpiA"
                     /locus_tag="ACH3L8_002256"
     CDS             4106..4873
                     /gene="tpiA"
                     /locus_tag="ACH3L8_002256"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFT4085346.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(4928..5683)
                     /gene="cdh"
                     /locus_tag="ACH3L8_002257"
     CDS             complement(4928..5683)
                     /gene="cdh"
                     /locus_tag="ACH3L8_002257"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFT4085347.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(5790..6779)
                     /gene="sbp"
                     /locus_tag="ACH3L8_002258"
     CDS             complement(5790..6779)
                     /gene="sbp"
                     /locus_tag="ACH3L8_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFT4085348.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(7099..8061)
                     /gene="pfkA"
                     /locus_tag="ACH3L8_002259"
     CDS             complement(7099..8061)
                     /gene="pfkA"
                     /locus_tag="ACH3L8_002259"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFT4085349.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(8242..9144)
                     /gene="fieF"
                     /locus_tag="ACH3L8_002260"
     CDS             complement(8242..9144)
                     /gene="fieF"
                     /locus_tag="ACH3L8_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFT4085350.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(9292..9792)
                     /gene="cpxP"
                     /locus_tag="ACH3L8_002261"
     CDS             complement(9292..9792)
                     /gene="cpxP"
                     /locus_tag="ACH3L8_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFT4085351.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            9942..10640
                     /gene="cpxR"
                     /locus_tag="ACH3L8_002262"
     CDS             9942..10640
                     /gene="cpxR"
                     /locus_tag="ACH3L8_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFT4085352.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            10637..12010
                     /gene="cpxA"
                     /locus_tag="ACH3L8_002263"
     CDS             10637..12010
                     /gene="cpxA"
                     /locus_tag="ACH3L8_002263"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFT4085353.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(12116..12790)
                     /gene="yiiM"
                     /locus_tag="ACH3L8_002264"
     CDS             complement(12116..12790)
                     /gene="yiiM"
                     /locus_tag="ACH3L8_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFT4085354.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT         3267 a         3375 c         3194 g         3086 t
ORIGIN      
        1 cttcgcgcca ttccttactg cttagagttt gctatgagac gagaacttgc catcgaattt
       61 tcccgcgtca ccgaatctgc ggcgctggct ggctacaaat ggttaggacg cggcgataaa
      121 aacaccgcgg acggcgcggc ggtaaacgcc atgcgtatta tgctcaacca ggtcaacatt
      181 gacggcacca tcgtcattgg tgaaggtgaa atcgacgaag caccgatgct ctacattggt
      241 gaaaaagtcg gtactggtcg cggcgacgcg gtagatattg ctgttgatcc gattgaaggc
      301 acgcgcatga cggcgatggg ccaggctaac gcgctggcgg tgctggcagt aggcgataaa
      361 ggctgcttcc tcaatgcgcc ggatatgtat atggagaagc tgattgtcgg gccgggagcc
      421 aaaggcacca ttgatctgaa cctgccgctg gcggataacc tgcgcaatgt agcggcggcg
      481 ctcggcaaac cgttgagcga actgacggta acgattctgg ctaaaccacg ccacgatgcc
      541 gttatcgctg aaatgcagca actcggcgta cgcgtatttg ctattccgga cggcgacgtt
      601 gcggcctcaa ttctcacctg tatgccagac agcgaagttg acgtgctgta cggtattggt
      661 ggcgcgccgg aaggcgtggt ttctgcggcg gtgatccgcg cattagatgg cgacatgaac
      721 ggtcgtctgc tggcgcgtca tgacgtcaaa ggcgacaacg aagagaatcg tcgcattggc
      781 gagcaggagc tggcacgctg caaagcgatg ggcatcgaag ccggtaaagt attgcgtctg
      841 ggcgatatgg cgcgcagcga taacgtcatc ttctctgcca ccggtattac caaaggcgat
      901 ctgctggaag gtattagccg caaaggcaat atcgcgacta ccgaaacgct gctgatccgc
      961 ggcaagtcac gcaccattcg ccgcattcag tccatccact atctggatcg caaagacccg
     1021 gaaatgcagg tgcacattct ctgagtgatt tgatcgattg agccttccag tccttcggga
     1081 ctggaatttt tttgttcgga gaacgaagat aaggcaagtc aatcaaaaca ggagaaaaac
     1141 atggctgatt gggtaacagg caaagtcact aaagtgcaga actggaccga cgccctgttt
     1201 agtctcaccg ttcacgcccc cgtgcttccg tttaccgccg ggcaatttac caagcttggc
     1261 cttgaaatcg acggcgaacg cgtccagcgc gcctactcct atgttaactc gcccgataat
     1321 cccgatctgg agttttacct ggtcaccgtc cccgatggca aattaagccc acgattggcg
     1381 gcactgaaac cgggcgatga agtgcaggtg gttagcgaag cagctggctt ctttgttctg
     1441 gatgaagtac cggactgcga aacgctatgg atgctggcaa ccggtacagc gattggccct
     1501 tatttatcga ttctgcaact aggcaaagat ttagatcgct tcaaaaatct ggtcctggtt
     1561 cacgccgcac gttatgccgc cgacttaagc tatttgccac tgatgcaaga actggaaaaa
     1621 cgctacgaag gcaaactgcg cattcagacg gtggtcagtc gggaaacggc agcggggtcg
     1681 ctcaccggac ggataccggc attaattgaa agtggggaac tggaaagcgc gattggcctg
     1741 ccgatgaata aagaaaccag ccatgtgatg ctgtgcggca atccacagat ggtgcgcgat
     1801 acacaacagt tgctgaaaga gacccggcag atgacgaaac atttacgtcg ccgaccgggc
     1861 catatgacag cggagcatta ctggtaagcg gttacttatc gataaacggc acgatgagca
     1921 aatccgcact catcttattg atcatcccgc gatatgccgg catcaaacgg ttgataaatg
     1981 agtgatgatg accacagaca aggaggtcgc actgctcttt ttgcataatt tccagcagtg
     2041 tttccggcat ttctccgcgt tcaatacgca gttttgtctt cggccattga atatttttcg
     2101 tcagtttata cagcttgtta tccgacttat tcttcaacaa ttgaagaata tcttctgttg
     2161 cagggaagta gatacccgga tacaactcgc ttaagccatc atcaatatga attaacgtca
     2221 ggtgagcgtc attatgtctg gcgagctcca gggctttatt taccagtaag gcatcttctt
     2281 cattcccgga aattgccacg ccaatgtgtt tataagccat gtttaactcc ttctaaagca
     2341 actccatcaa gctaacgaac gcagtgataa ctcaaaaata atcatctcgg cctggcatga
     2401 gaaagtgaag gccgcatcaa gctcaacttg cccctccaga tccttaatct tataggccac
     2461 gctggcccct tcatctgcag aggatgcagc ggcggcaagc tctttcagtt tggtcataaa
     2521 agactctgct tcttcgcggg tggcaaaaaa gcgcgaaaac ttactggtac agttgtcgtt
     2581 atcaatcacc gtaccgatat caatcgcaca tcctttagta ctgcatttat ctacgacatc
     2641 tttcataggg cacctctgta tattcgcctc tctgtcgatt acgacttaag aataatgcgc
     2701 cgttaaggga agaacaaaca agtaatgatc ttatggatta ccattttttt tgatattgct
     2761 taaggtgaag agttcagaag gattttccgg gtattgtcat ttgcatcgtg gaaataacaa
     2821 tcccggaaaa aaatagcagt aagagatata ttaaaggtta attacttgat ttattgtcgg
     2881 ctttatactt cacatcctga gtatctttac catatttatt ttcgccttgt gtgccaacaa
     2941 acgcaccaag atcgataagc atcatcacca gaatcaacgt cgggacaaaa cgccccaccg
     3001 cccattgcca gacacccggt aaaatcgctc agttacccgc cagcagcatc cacgccacaa
     3061 tcatcagaaa tgcccatgcg ccggaacgcc cgcgatcatg caagcgctta acagttactg
     3121 ccgctgttgg ccagagcaag cacacaaggc aaaacgccgc ggtctgaata tcgagtagat
     3181 tcttacccgc cagtgagaaa agcaccagca tgcctgcgaa ccacaggcct atccaaatcc
     3241 agaaatcacg gcgtccaata cgccctttaa atgagaataa ccattgctgt atggtcatgt
     3301 aagttccttg atggttgtct tttccaggat tctacccttt tgacaagggc gacagatatc
     3361 gttttaatcg gagccagtca taacaaaagg tactgtcaat gaagccaggg tgtacgctgt
     3421 tttttctctt atgttctgca ttaaccgtta caacaacggc gcatgcacaa acaccagata
     3481 cggcaacgac cgcgccttat ctgctggctg gagcccctac tttcgatctc tccatcagcc
     3541 agtttcgaga agactttaac agccagaatc ccagcctgcc actgaacgaa tttcgtgcca
     3601 tcgacagcag tcccgacaaa gccaatctca ctcgtgctgc aagtaaaatt aacgagaact
     3661 tgtatgcttc tacagcgctg gagcgcggta ccttaaaaat caaaagcatt caaatgacct
     3721 ggctacccat ccaggggcca gagcaaaaag cagcgaaagc gaaagctcag gaatacatgg
     3781 cagcagtgat ccgcacactc accccattaa tgaccaaaat acaaagccag aaaaaactgc
     3841 agtcgctact aacggcgggg aaaaacaaac gttattacac cgagacagaa ggtgcactgc
     3901 gttatgttgt cgcggacaac ggcgaaaagg ggctgacctt cgctgttgaa ccgattaagc
     3961 tggcgctatc tgaatcgctt gaaggtttga ataaatgaca aaaagcaaag cctttgtgcc
     4021 gatgaatctc tatactgttt cacagacctg ctgccctgcg gggcggccat cttcctttat
     4081 tcgcttataa gcgtggagaa ttaaaatgcg acatccttta gtgatgggta actggaaact
     4141 gaacggcagc cgccacatgg ttcacgagct ggtttctaac ctgcgtaaag agctggcagg
     4201 tgttgctggc tgtgcggttg caatcgcacc accggaaatg tacatcgata tggcgaagcg
     4261 cgaagctgaa ggcagccaca tcatgctggg tgcgcaaaac gtggacctga acctgtccgg
     4321 cgcattcacc ggtgaaactt ctgccgctat gctgaaagac atcggcgctc agtacatcat
     4381 catcggccac tctgaacgtc gtacttacca caaagagtct gacgaactga ttgcgaaaaa
     4441 attcgcggtg ctgaaagagc agggtctgac tccggttctg tgcatcggtg aaaccgaagc
     4501 tgaaaacgaa gcgggaaaaa ctgaagaagt ttgcgcacgt cagatcgacg cggtactgaa
     4561 aactcagggt gctgcggcat tcgaaggtgc ggttatcgct tacgaacctg tatgggcaat
     4621 cggtactggc aaatctgcaa ctccggctca ggcacaggct gttcacaaat tcatccgtga
     4681 ccacatcgct aaagttgacg ctaacatcgc tgaacaagtg atcattcagt acggcggctc
     4741 tgtaaacgcg tctaacgctg cagaactgtt tgctcagcca gacatcgacg gcgcgctggt
     4801 tggcggtgct tctctgaaag ctgacgcttt cgcagtgatc gttaaagctg cagaagcggc
     4861 taaacaggct taagtctgac aggtgccgga tttcatatcc ggcacttact ttccttaact
     4921 cctcgcctta acgcaaaatc tcacactcat gatcctggat ttcctcggct gaagcacggt
     4981 tcagcgtcag tagatttcgt tgtgtcgcca gcaacacaaa cgaattatca ctctgccgta
     5041 ccatcgccag cccgtagctt cccatatgtt cccgcgcctc aggcacttct tctgcaagca
     5101 tcataaatgg gctgcgttgt accagttcgc tttccgttac ccgacgcgcc aggtattcat
     5161 gcccgcgcaa accacctggc agtggcaacc agcggctgct gatgttcgcc agatttttat
     5221 ccagttgttc gcgcacatca ggacgaatac aagagatatg aatatgaaaa tggttttgcg
     5281 tatgcccggt gcgggagttg atcgccaaag aaaccgcgcg atcgggaacc ggctggccgt
     5341 attttttgct cataaaatca cgcgcctgcc aggccaacca aaagaagttc ggcgttgaag
     5401 gatcggtcaa caaaggactt tcagtaccgt taatacgata cgttggcatc aacagatatt
     5461 gcagtggacc attaagatct tttaaaacca cgtatccggc attgggtttg acttccgcac
     5521 atggcgaagg attttgattt tgctgctgat tgggcaaaca ttcctcaagg acaatcttac
     5581 gtaatgtatc cgactcttca ccggttaatt tccagtaacc aataccggca gccacaacgg
     5641 cgataactat catcaccaaa aaaagaagac ccgctttttt catctttttt tccctgtacc
     5701 tcaaagagat gcaagggtaa cgcaaaatcg tgacaaataa aaaacccggt cgcgagaaac
     5761 caccgggttt gataattatc ctagggaaat cagcgtttgc tgatctgatc gaacgtaccg
     5821 ccgttagcaa aatgctcttt ttgcgctttc gtccagccgc cgaactcttc atcaatggtg
     5881 aataacttca gctttggaaa cgcattttcg tactttttcg ctacttcagc gtcgcgcgga
     5941 cggtagtagt ttttcgcggc aatttcctga ccttctggcg agtagagata tttcaggtag
     6001 gcttccgcca cctctttggt gccttttttc tcgaccactt tatcgaccac cgacaccgtt
     6061 ggctctgcga ggatagactc actcggcgtg acgatttcga atttatcttt ccccagttca
     6121 ttcgctgcca gcagagcttc gttttcccag gcgatcagta catcgccaat cccgcgctcg
     6181 acaaaggtgt tagtggagcc gcgcgcgcca gaatccagaa cttcgacgtt tttatacagt
     6241 gcccgaacaa aatcctgtgc ttttgcctga tcgttgttgt tgtgatgcag cgcgtagccc
     6301 caggcagcca ggtagttcca gcgtgcgcca ccagagcttt tcggattagg cgtgatcacc
     6361 gaaacacccg gtttaatcag atcgttccag tcatggatct gcttcggatt gcccttacgc
     6421 accaggaaaa caatggtgga agtgtacggt gcggagttat ccggcagacg tttgatccac
     6481 tctttatcaa tccgcccgcg ttccgcaatc gcgtccacgt cataggccag agccagcgtg
     6541 acaacatcag cttcaatacc gttgattacc gacgtcgctt gtttacctga accaccgtgt
     6601 gactgacgaa tcaccacgtt atcaccagtt tgctgtttcc agtgggcgct gaatgccttg
     6661 ttgtactgtt cgtacaattc gcgcgttgga tcatatgaaa cgttaagaag ctgaatatcc
     6721 tttgccataa cgctggttgc cgccagcaaa aatgttaacc ctacgcccca cttgttcatc
     6781 gcccgactct cttatgttgt gttgtgatga gcaaagcgtg ccagaaggtt aaccaaacat
     6841 taaagaataa aaaaagattg gctataactt gcgggtatat gttgagggat taaaaaggcg
     6901 gaagaaatcc gcctcatatt gctgacaaag tgcgctttgt ccatgccgga tgcggcgcga
     6961 acgccttatc cggcctacaa aagtttgcaa attcaataaa ttgcagaatt catgtaggcc
     7021 tgataagcga agcgcatcag gcatttttgc ttctgtcatc ggtttcaggc taaaggaatc
     7081 tgcctttttc cgaaatcatt aatacagttt tttcgcgcag tccagccagt cacctttgaa
     7141 cggacgcttc atgttttcga tagcgtcgat gatgtcgtgg tgaaccagct gttcgttctg
     7201 gatacctaca caacgaccgc cgtaacctgc cagcagcaga tcgatagcgt aagcgcccat
     7261 acgggaagcc agaatacggt cgtaaggcac cggagaacca ccgcgctgga tgtggcccag
     7321 cacagttgcg cgggtttcac gaccggtttc tttctcgatg aaatgcgcca gttcgtcaac
     7381 atcacacata tgttcggtaa tcgccacgat cgcgtgtttt ttacctttcg cgatacccgc
     7441 tttgatttcg tttaccaggt cttcacggct gaattcaact tccggaacta caacgaattc
     7501 acagccaccg gcaatggccg cagccagggt cagatcgcca caataacggc ccatcacttc
     7561 caccacggaa atacgctggt gagaagaaga ggtgtcacgc agacggtcga tcgcttctac
     7621 aacggtgctc agcgcagtga agaaaccgat agtgtagtca gtgcctttga tgtcgttgtc
     7681 gatagtgccc ggcagaccga tgcacgggaa gcccatttcg gtcagacgca ttgcacccat
     7741 gtaggaaccg tcaccgccga taaccaccag cgcgtcgata ccacgttttt tcaggttttc
     7801 gatagccacg gcgcggatgt tctcgtcgcg gaattccggg aaacgcgcag aaccgaggaa
     7861 cgtaccgcca cggttgatca tgtcagacac gctgtaacgg tctagctgta ccatacggtc
     7921 ttcatacaga cccagatagc cgtcataaat acccattact tccagacctt ctgtcagcgc
     7981 agaacgaaca accccgcgaa ttgcggcgtt catgcctggc gcatcaccgc cgcttgtcaa
     8041 cacacctatt ttcttaatca tgactacctc tgaactttgg aatgcaaaat gaaatctgtt
     8101 gccggaagtc ttcttgcaca tcgaagtgat ccaacgaatg tgcaaatagt ataacaatca
     8161 cttcctgctg aattgattca ggtcaggcca aatggcggta ttttatacac aaaatgcggg
     8221 tctggctctc ttttatactg attatgaaag catagaccgt ttaccctccc tgggtacgac
     8281 ggaacagggg tcctgatgga taatcacatc cgatcccgga aaacgccgta aaatagcctg
     8341 ctctacctga tccgccacca tatgtgcctg aaccaaaggc agagagtctt ccatttccaa
     8401 atgaatctga ataaagcggg tcggccctga ctgccgcgtg cgaagatcgt gagcgccgct
     8461 aacacccggc caggaagtca cgatatcaat aatttcttgc cgttcttcat caggcaatgc
     8521 gcgatccagt aatgactgta ccgcctcata tcccatgcgt aacgcgctat ataaaatata
     8581 gatgccgatt cccaatgcaa acagagcatc ggcgcgatgc cagccgtacc aagacaaccc
     8641 cagcgccagc agaattgcgc cgttcatcat aacatcagac tggtaatgta gcatatcagc
     8701 ccgcaccgcc tggctttgcg tccggcgcac cacccaccgc tgaaacgaga caaggataat
     8761 cgtacaaatt agcgccacaa ttgtcacgat aaccccgacg cctggatctg tcatcggtgt
     8821 tggagatacc agatgttgaa tacccgtcaa aaacaggaat agtgccgaac cggagataaa
     8881 catactttgc gccagcgccg cgagggactc agctttaccg tgaccaaacg agtgattatc
     8941 gtcggcaggt tgcagggaat atcgcaccac cagtaaattc gtcaacgacg cgccgatatc
     9001 caccagcgaa tccaccagcg cggcgagaat actcaccgac ccggtatacc accatgcaaa
     9061 aattttaatc agcaatagca acgaagccat cgccgtcgca gcaatcgccg cccgactgac
     9121 cagccgtcca taagattgat tcataaatac tcccgctatc aactgacgct agtataacgg
     9181 aagcaaatca tctgcaatgc attaagcagc aggcaaattg aggataaaaa aaaccccaca
     9241 tcatgtgggg gaagacaggg atggtgtcta tggcaaggaa aacagggttt actactggga
     9301 acgtgagttg ctactactca atagcttcaa cgatgaactt ttttgccatt gcgtcacgtc
     9361 acgcaactgc tccattcgtt gttgatgttt ctcgtttaaa accgcttgct gctccggcgt
     9421 taacaggcga tacatttggt tgcggacttt tgccatctca acctgacgag caatttgctc
     9481 attcgccatt ttttctgcct gtgcgcgcac agcgttttca tcaaaatttt ctgcggtgac
     9541 aaggcgatgc attgtctcca gttcgctaac attaacagga ggctgttcgt gccgggcctg
     9601 ttgcataaga tctcgcatct gctgacgctg atgttcggtt aaacttatgc cgtcgaacat
     9661 atggctctgc gtactgcgct gcgtaagttc ttcacccgga tgccagttat cgcctgaacc
     9721 gacttcagca gcgtggctta atgaactgac tgccagcgtt gaggccatga cggcagcggt
     9781 aactatgcgc atcatttgct cccaaaatct ttctgtcgcg attcaacgat agagagttta
     9841 cgattcaggc tgcaaacatg cgtcaggggg tgtaaaacaa cgtaaagtca tggattagcg
     9901 acgtctgatg acgtaatttc tgcctcggag gtatttaaac aatgaataaa atcctgttag
     9961 ttgatgatga ccgagagctg acttccctat taaaggagct gctcgagatg gaaggcttca
    10021 acgtgattgt tgcccacgat ggggaacagg cgcttgatct tctggacgac agcattgatt
    10081 tacttttgct tgacgtaatg atgccgaaga aaaatggtat cgacacatta aaagcacttc
    10141 gccagacaca ccagacgcct gtcattatgt tgacggcgcg cggcagtgaa cttgatcgcg
    10201 ttctcggcct tgagctgggc gcagatgact atctcccgaa accgtttaat gatcgtgagc
    10261 tggtggcacg tattcgcgca atcctgcgcc gttcgcactg gagcgagcaa cagcaaaaca
    10321 acgacaacgg ttcaccgaca ctggaagttg atgccttagt gctgaatcca ggccgtcagg
    10381 aagccagctt cgacgggcaa acgctggagt taaccggtac tgagtttacc ctgctctatt
    10441 tgctggcaca gcatctgggt caggtggttt cccgtgaaca tttaagccag gaagtgttgg
    10501 gcaaacgcct gacgcctttc gaccgcgcta ttgatatgca catttccaac ctgcgtcgta
    10561 aactgccgga tcgtaaagat ggtcacccgt ggtttaaaac cttgcgtggt cgcggctatc
    10621 tgatggtttc tgcttcatga taggcagctt aaccgcgcgc atcttcgcca tcttctggct
    10681 gacgctggtg ctggtgttga tgttggtttt gatgttaccc aagctcgatt cacgccagat
    10741 gaccgagctt ctggatagcg aacagcgtca ggggctgatg attgagcagc atgtcgaagc
    10801 ggagctggcg aacgatccgc ccaacgattt aatgtggtgg cggcgtctgt tccgggcgat
    10861 tgataagtgg gcaccgccag gacagcgttt gttattggtg accaccgaag gccgcgtgat
    10921 cggcgctgaa cgcagcgaaa tgcagatcat tcgtaacttt attggtcagg ccgataacgc
    10981 cgatcatccg cagaagaaaa agtatggccg cgtggaactg gtcggtccgt tctccgtgcg
    11041 tgatggcgaa gataattacc aactttatct gattcgtccg gccagtagtt ctcaatccga
    11101 tttcattaac ttactgtttg accgcccgct attactgctg attgtcacca tgttggtcag
    11161 tacgccgctg ctgttgtggt tggcctggag tctggcaaaa ccggcgcgta agctgaaaaa
    11221 cgctgccgat gaagttgccc agggaaactt acgccagcac ccggaactgg aagcggggcc
    11281 acaggaattc cttgccgcag gtgccagttt taaccagatg gtcaccgcgc tggagcgcat
    11341 gatgacctct cagcagcgtc tgctttctga tatctctcac gagctgcgca ccccgctgac
    11401 gcgtctgcaa ctgggtacgg cgttactgcg ccgtcgtagc ggtgaaagca aggaactcga
    11461 gcgtattgaa accgaagcgc aacgtctgga cagcatgatc aacgatctgt tggtgatgtc
    11521 acgtaatcag caaaaaaacg cgctggttag cgaaaccatc aaagccaacc agttgtggag
    11581 tgaagtgctg gataacgcgg cgttcgaagc cgagcaaatg ggcaagtcgt tgacagttaa
    11641 cttcccgcct gggccgtggc cgctgtacgg caatccgaac gccctggaaa gtgcgctgga
    11701 aaacattgtt cgtaatgctc tgcgttattc ccatacgaag attgaagtgg gctttgcggt
    11761 agataaagac ggtatcacca ttacggtgga cgacgatggt cctggcgtta gcccggaaga
    11821 tcgcgaacag attttccgtc cgttctatcg gaccgatgaa gcacgcgatc gtgaatctgg
    11881 cggtacagga ttggggctgg cgattgttga aaccgccatt cagcagcatc gtggctgggt
    11941 gaaggcagaa gacagcccgc tgggcggttt acggctggtg atttggttgc cgctgtataa
    12001 gcggagttaa actccgcatt tgtaggcagg ataaggcgtt tacgccgcat ccggcatttg
    12061 agcaggatgc ctgatgcgac gctgatagcg tcttatcagg cctacactcc gggggttatt
    12121 ttccccacaa ccggcgggaa aaatcttcga tcttgccgct cagtcggcgc ttttgcatcg
    12181 tccgcgtcca gcttttcgat aacccggcgg cggagagtaa acggtgatac tgatcgtcat
    12241 caaacggcat atgccatgcg atggcggcag cctcctgcac ggtcacatcg ctgacacggg
    12301 aaaccaactc cagcggcgcg tccgcagaca ctttccctgg cgctatcacg ctatacaacc
    12361 accccacctt acctgtgttt tgcatcaact gcgctatatc gctgatatca aaatggtaat
    12421 tgagtttata gcaaggcgag cgcggctggc tgacctgaat taatgcctct ccccagcgga
    12481 aaatatcgcc catatgaaca ttactttccg tgagaccgtc ggttgagaga ttttcgccaa
    12541 acgcaggcgc aacaaacaac tccgcctgtt ccggaaattc ccgcacccag tagagatagt
    12601 gctcacgagg ataatgacac agcgctctgt ctggcccgcc gtgaaccttt ttctccgcct
    12661 gctcgtcacc ttccagcccc agctccgtca gcatcaactc accatcaacc tggattttgg
    12721 caattgcgct gggtttgcct tcgggataag cctgaatctt gcctgtgtat acatcaaccg
    12781 gatatcgcat ctgttccctc tccacgagaa atttccccat tagaccatat ttattttatg
    12841 tcagccgtgc tgttttcgcg gtaagtatcc aggcaggaac tcaatagcaa accggacgaa
    12901 cgttagttca tccggctgtg gg
//
LOCUS       DAZXUK010000102        12907 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZXUK010000102 DAZXUK010000000
VERSION     DAZXUK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12907
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.102"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACH3L8_002265"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACH3L8_002265"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFT4085355.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="ACH3L8_002266"
     CDS             complement(1806..3224)
                     /locus_tag="ACH3L8_002266"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFT4085356.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEVLKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="ACH3L8_002267"
     CDS             complement(3378..3722)
                     /locus_tag="ACH3L8_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFT4085357.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACH3L8_002268"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACH3L8_002268"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFT4085358.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5653)
                     /locus_tag="ACH3L8_002269"
                     /pseudo
     CDS             complement(4712..5653)
                     /locus_tag="ACH3L8_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="ACH3L8_002270"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="ACH3L8_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFT4085359.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDTLSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="ACH3L8_002271"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="ACH3L8_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFT4085360.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACH3L8_002272"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACH3L8_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFT4085361.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="ACH3L8_002273"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="ACH3L8_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFT4085362.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="ACH3L8_002274"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="ACH3L8_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFT4085363.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="ACH3L8_002275"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="ACH3L8_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFT4085364.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACH3L8_002276"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACH3L8_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFT4085365.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11368..12483
                     /gene="ydeE"
                     /locus_tag="ACH3L8_002277"
                     /pseudo
     CDS             11368..12483
                     /gene="ydeE"
                     /locus_tag="ACH3L8_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="ACH3L8_002278"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="ACH3L8_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFT4085366.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            12705..12808
                     /gene="mgtT"
                     /locus_tag="ACH3L8_002279"
                     /pseudo
     CDS             12705..12808
                     /gene="mgtT"
                     /locus_tag="ACH3L8_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
BASE COUNT         3402 a         3299 c         2948 g         3258 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcacctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tacactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatg
//
LOCUS       DAZXUK010000103        12733 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZXUK010000103 DAZXUK010000000
VERSION     DAZXUK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.103"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1038
                     /locus_tag="ACH3L8_002280"
     CDS             <1..1038
                     /locus_tag="ACH3L8_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFT4085367.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1039..3204
                     /locus_tag="ACH3L8_002281"
     CDS             1039..3204
                     /locus_tag="ACH3L8_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFT4085368.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKEADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3218..4129
                     /locus_tag="ACH3L8_002282"
     CDS             3218..4129
                     /locus_tag="ACH3L8_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFT4085369.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4129..5424
                     /locus_tag="ACH3L8_002283"
     CDS             4129..5424
                     /locus_tag="ACH3L8_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFT4085370.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5469..5699
                     /locus_tag="ACH3L8_002284"
     CDS             5469..5699
                     /locus_tag="ACH3L8_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085371.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5677..6177
                     /locus_tag="ACH3L8_002285"
     CDS             5677..6177
                     /locus_tag="ACH3L8_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFT4085372.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6177..7595
                     /locus_tag="ACH3L8_002286"
     CDS             6177..7595
                     /locus_tag="ACH3L8_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFT4085373.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7595..8443
                     /locus_tag="ACH3L8_002287"
     CDS             7595..8443
                     /locus_tag="ACH3L8_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFT4085374.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8443..8898
                     /locus_tag="ACH3L8_002288"
     CDS             8443..8898
                     /locus_tag="ACH3L8_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFT4085375.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8901..9593
                     /locus_tag="ACH3L8_002289"
     CDS             8901..9593
                     /locus_tag="ACH3L8_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFT4085376.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9603..10934
                     /locus_tag="ACH3L8_002290"
     CDS             9603..10934
                     /locus_tag="ACH3L8_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFT4085377.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10935..12665
                     /locus_tag="ACH3L8_002291"
     CDS             10935..12665
                     /locus_tag="ACH3L8_002291"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFT4085378.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT         3436 a         3144 c         3355 g         2798 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
     1081 gacgcagact ggatggcgag cgatgaagcc agaaccgagg cgacaaatga cctgtatttt
     1141 agccgagtgt cgcaatggga tgactggcta tcaaactaca ctaccctgca atatcgcgga
     1201 caattcgatg ttgttcgccc ggaggtcagg aaactggtcg cagagatgcg ccggaaccct
     1261 gtcgacgttc tcttccgacc caaagacggc gctaatcctg atgcagccga tgtattgatg
     1321 gggatgtatc gtactgatat gcgccataac acggcaaaga ttgccgttaa cgttggcgtt
     1381 cgtgagcaga tagagtcagg cgttggtgca tggcgtctgg tcacgcagta cgaagacaac
     1441 gatccaacaa gcaacaatca ggtaatccga cgcctgccaa tccatgaagc ctgctcacac
     1501 gtcatatggg acgccaacag caagcagatg gataagagcg acgctaagca ctgcacggtg
     1561 attaacgcct tgtcgcgcaa tggctggaaa gagttcgcag aggattacgg tattgacccg
     1621 gataccctgc catctttcca gaatcctaac gatacatggc tattcccgtg ggtgtcgaat
     1681 gatgtcgtct acgtcgctga gtattacgag gtagaagagg agaaggagaa ggtcttcatc
     1741 taccgcgacc cgctgacagg tgagccggtc agctattacc agcaggatat caaagacgtc
     1801 atcgacgacc tggctaatcg tggattcatt aaggtagcag agcgcaaggt gaagcgtcgg
     1861 cgtgtgtata agtcgatcat cacctgtacg cagatactga aagaccgcga gaagatagcc
     1921 ggagagcata ttccaatcgt tccagtgtat ggcgaatggt cattcgctgg tgacaaggag
     1981 tgctacgagg gtgtggtaag gctgacgaaa gacggtcaac gccttcgtaa catgattatg
     2041 tcgttcaacg ccgatattgt tgctcggtca ccgaagaaga aaccgacctt cttccctgag
     2101 caaatcgaag gctacgaata catgtacggc ggaaatgatg actatccgta ctatctgcag
     2161 aacaggactg atgaaaacgg taacgacctg ccgattggtc caatctccta catggaaaac
     2221 cctgaagtgc cgcaagccaa cgcttacatg cttgaggctg ccaccaacgc agtgaaagaa
     2281 gtggctagtc ttggcgtgga tgcgcaggcg gcaaatggtc aggtcgcttt tgataccgtc
     2341 aatcaactga acatgcgggc agaccttgag acatacgtgt ttcaggataa cctggctacc
     2401 gcaatgcgac gtgatggcga gatttatgcc tcaatggtca acgatattta tgacgttcct
     2461 cgtcatgtaa cgctgacact tgaagatgga agcgagaaag acgttcaact ctatgcgcaa
     2521 gttgtcgatt accagtccgg caatgtggtc acactcaacg acattcgcgg tcgatatgag
     2581 tgctatacag acgttgggcc atccttccag agtatgaagg aacagaaccg cgcagagatt
     2641 caggagttgc tcaccaaggt tccgcaaggt actccagagt tccagatgct gatgctgcaa
     2701 tacttcacgc tgcttgacgg taaaggcgtc gagatgatgc gagagtacgc gaacaagcaa
     2761 ctggtgatga tggggctgaa gaaaccagaa acacctgaag agatggagat ggtgcagcag
     2821 gcacaacaac agccgcagca gccatcagca gagcaaattc aggcgcaggg tatccttctg
     2881 caaggtcagg ctgaattgct caaggcagag aaccaacagg cgcagattca ggttgaagct
     2941 gccaaggttg aagcccaaaa ccaactcaac gccgcgaaga ttgcagaaat attcaacaat
     3001 atggacctcg acaagcaggc agaactgcgt gagtacctca ggctcgtagg tcaattccag
     3061 caacagcgca gcaaagatgc tcgtgctaac gctgagctgc ttcttaaaga agcagaccag
     3121 actcattcac aacgcatgga tttcgcgaat cttatgcgtc aagttcaaat cccctccggc
     3181 ggagtagccg agacacctca ataagagaga gttaatcatg gaccaaacca ccgacattca
     3241 ggcttctgaa gaattaaccc tgcccggcaa tcatgcagcg gcatctgctg atggcttagt
     3301 tgtcgataat gccaacgaca gcgcaggtca ggaagaaggc tttgagattg tccggaaaga
     3361 cgatgagaaa ccaaaacaag acccggcaac taatgctgaa tttgcccgtc gccgcatcga
     3421 acgcaaacgc cagcgtgagc ttgagcagca gatggaagcg gttaagcgtg gagagttgcc
     3481 ggagcacctg cgggtgaacc ctgagttacc aaaacaacca gaccctaacg attatctttc
     3541 cgaagatgca ctggctaagt acgactatga ccagagccgc gcactggctg ccttccagca
     3601 ggcaaacagt gaatggcaga tcaaggctat ggacgcacga agccaggctg tcgccgagca
     3661 gggtcgcaaa actcaggagt tcacccagca atcagcgcaa tacgtcgagg cggcccgtaa
     3721 gcactacgag gcagcggaaa agctcaatat ccctgactat caggagaaag aggatgcatt
     3781 catgcaactg gtgccgccag cagtcggtgc cgacatcatg cgcctcttcc cggagaaatc
     3841 cgccgctctc atgtatcacc ttggtgctaa tcctgagaaa acacgccagt tgctggcgat
     3901 ggacgggcaa tccgcgctga ttgaactcac tcgactgtca gaacgtttaa ctctcaagcc
     3961 tcgagccaag cctgtttcag aagccccgtt acctgatgaa cccattcagg gacatgctgt
     4021 tgctgcaaat atctctgcga ttgaaaagca gatggaggcg gcagcaaaca aaggggatgt
     4081 agagacgtac cgcaagctca aggcgcaact gaataaagga attcgataat ggcattaaat
     4141 gaaggtcaac tggtcacgta tgctctggat gaaatcatcg gaaccgtcca gaacctgacg
     4201 ccaatggcgt caaaagtgac aaaatacccc cctccggcag aatccatgca gcgttcaagc
     4261 aacaccgtgt ggatgcctgt tgagcaggaa gcgccaaccc agactggctg ggatttaact
     4321 ggcaacgcaa ccgggattct ggaactctcc gtgaagtgca acatgggcga tccggataac
     4381 gatttcttcg agcttcgtgc agatgacctg cgtgatgagc gttcttaccg tcgccgcatc
     4441 caggcatccg ccaaaaaact ggcgaataac attgagtcag cgattgccaa acaggcaact
     4501 gaaatgggct cgcttgttgt tcacgatacc cgcgcaattg gtccatcgac tggcctgcct
     4561 ggctgggatt ttgtgtctga tgcagagcga ctgatgttct cccgtgagct aaaccgcgac
     4621 atgggcatca gttacttcct gaaccctgac gattaccgca aagcaggccg caacctggta
     4681 gatggtgaca tcttcgggcg cgttcctgaa gaagcatatc gaaacggtac tattcagcgt
     4741 cagattgctg gctttgatga aattcttcgc tcaccgaaac ttccggcagt taccaagtca
     4801 accgcgactg gtgtaactgt ttctggtgcg cagaagttta agccgcaggc atacaccctt
     4861 gataccgatg gtaacaaaga gaacgtcgac aaccgtgttg caacggtgac cgtatcctcc
     4921 accaccggat ttaagcgcgg cgacaaaatc agcttcattg gtgtgaagtt cctgtctcag
     4981 atggcgaaga atgtgctgac tgatgacgcg actttctcaa tcacccgtgt gatcgatggt
     5041 actcacatcg aaatcacgcc gaagccgatt gcgctggatg acgcgtcact gacaaaagaa
     5101 gagaaggctt acgctaacgt aaacacctct cttgctgata ccactccggt aaacgttctg
     5161 aacgtggcaa caaccaccgc taacgtgttc tgggctgatg actcaatccg cctgctgtct
     5221 cagccgatcc cggtaaccca tgaactgttt gctggcatga aaacgtcttc cttcagcatt
     5281 ccaggtattg gtgttaacgg catcttcgca acgcagggtg atatcaacac tctgtctggt
     5341 aagtgccgta ttgctgtgtg gtattcagca tgtgctgtac gaccagaggc aattggtgtt
     5401 ggtctgccta accagaccgc gtgataacca gagggagctt cggctcccgt ttttatctgg
     5461 agacaagcat gacacacatg atctttcgtc atggcgacat gaagaagtgg aaaggcgttg
     5521 gctacgactt tgaaatcgtg aaagccgaag agcttcagga atatctggat gctggctggt
     5581 tttcacatcc tgatgacctt ttgaaggatg ttgcagagcc agagccagaa gaaaaacagc
     5641 gtaaaaagcc tggtcgaaaa cctaaggcgg cagcagatga acctgacaac gaagggtgat
     5701 ttagttcttg cggcattacg taagctcggt gttgcatcaa atgccacgtt aaccgatgtc
     5761 gaaccgcagt ctatggaaga cggcgtcaac gaccttgaaa tgatgatggc tgaatggctt
     5821 ggcggtgatg cgtcacctgg tatcaacgtt ggctacattt ttgctgatgc agatgttgct
     5881 ccggatccgg gcgatgagca cggtttatca aataacgcta tcaatgccgt cattttcaac
     5941 cttgcctgcc gaattgctcc agattatgcg ctggaagcgt ctgcaaaact tataaccact
     6001 gccagatacg ggaaagagcg actcgtcaaa ctgtctgcaa tggacagggc aaaagcagct
     6061 aaatgtaagt ccggttatcc aaaccgtatg cctgttggta gtggtaacca gttggcgaag
     6121 tggaatggtt ggaattactt ccaccgaaag gaaccttgcg ataacgggag cgaataatgc
     6181 ctattcagca acttccgctt atgaaaggtg tcggcaaaga cttccgaaac gccgactata
     6241 tcgactatct gccagtgaat atgctggcta cacccaaaga aatcctgaac agcagcggat
     6301 atcttcgctc attcccgggc attgccaaac gttctgacgt gaacggtgta tctcgcggcg
     6361 tcgagtacaa catggcgcag aatgctgttt atcgcgtgtg tggcggcaag ctgtacaaag
     6421 gagaaagtga agtcggtgat gttgccggaa gtggtcgtgt atcaatggcg catggtcgga
     6481 catcacaggc ggtaggcgtt aatggtcaac tggtcgagta tcgctatgat ggcacggtca
     6541 aaaccgtctc aaactggcct acagacagtg gattcactca gtatgagtta ggttcagttc
     6601 gcgacattac gcgcttacgt gggcgttatg cgtggtcaaa agacggaact gattcatggt
     6661 ttatcactga ccttgaagac gaatctcatc ctgaccgtta cagcgcacaa tatcgtgccg
     6721 agtctcagcc tgacggcatc atcggtatcg ggacatggcg agacttcatc gtctgctttg
     6781 gttcatcgac tattgaatat ttctccctga ctggcgcaac caccgttggt gccgctttgt
     6841 atgttgcaca accatcgctg atggtgcaga aaggcattgc cggaacttac tgcaaaacgc
     6901 cattcgctga ttcgtatgcg tttatcagca atccggcaac gggtgcgccg tctgtgtata
     6961 tcatcgactc cggtcaggtg tcaccaattg ccagcgcgag cattgagaaa atcctccgct
     7021 cctacactgc tgatgaactg gctgatggtg tgatggaatc gctgcgattt gatgctcatg
     7081 agttgctgat tatccacctt ccgcgtcacg tcctcgtgta cgacgcatct tcaatcgcca
     7141 atggcccaca atggtgtgtg ctgaaaacag gtctgtatga cgatgtgtac cgcgctatcg
     7201 acttcgttta cgaaggcaat cagataacgt gcggcgataa gctggaatct gttaccggga
     7261 aactgcaatt cgatatcagc agccagtacg acaagcaaca ggaacacctg ctgtttactc
     7321 cactcttcaa agcggataac gccagagtgt tcgaccttga ggttgaatcg tcaactggcg
     7381 ttgcgcagta cgccgaccgc ctttttctct ctgcaaccac tgactgcatc aattacgggc
     7441 gtgagcagat gattgagcag aatgaaccgt tcgtttacga caagcgtgtt ttgtggaagc
     7501 gagtagggcg catcaggaaa aatgtcggct tcaaattgcg cgttatcacg aagtcacctg
     7561 tcactctgtc tggcgctcag ataaggattg agtaatggct gattcgaatc tcaatgtgcc
     7621 ggtaatcatc caagctacgc ggctcgatac atcagtcctt ccacgcaata tcttctcgca
     7681 gtcgtatctg ctttacgtta tcgcacaggg cactgatgtt ggtaacgtgg ctaacaaagc
     7741 caacgaggcc ggacagggcg cttatgatgc acaggtcagg aacgatgagc aggatgtgat
     7801 tctcgctgac catgagcagc gaatttctgc tgcggaagca acgcttgtta atcatgagga
     7861 gcgaatcaga caggcagaat caactcttca ggaccatgaa acacgaatag ctcagaatga
     7921 aagcgatatt tcgtcgcttg acacaagagt tcagtcgctg gagtcgcagg tttcagacca
     7981 tgaaacgcgc atcgatgctc tggagtatgc aaccacacgc aagaagtcag aggttgttta
     8041 ctctggtgta tctgtaacca tcccgacagc gccgaccaac cttgttagcc tgctgaaaac
     8101 gctcacaccg tcatccggct cgttggcacc attcttcgac accgttaaca acaagatggt
     8161 tgtgttcaac gagaacaaaa ccctgctctt caagttgtcg attgtcggca cgtggcccag
     8221 cggaaccgcc aacaggtcaa tgcagctaac cttttccggc tctgttcctg acacactggt
     8281 cagcagtcgt aatgcggcga caacaaccga caacatcctg ttagctacgt tcttcagcgt
     8341 ggataaagac ggctttcttg ccacaaatgg cagtacgtta accattcagt cgaatggtgc
     8401 ggcgtttact gccacaacca tcaagataat cgcggagcag tgatgattac attcaaacca
     8461 acgcgaaaca tcgacctgat agaagcagtc ggaaatcacc ctgacattat cgccgggagc
     8521 aacaacggtg atggatacga ctacaaacct gaatgccgtt actttgaggt gaacgtgcac
     8581 ggtcagtttg gcggcattgt ttactatcag gagattcagc cgctgacatt agattgccac
     8641 gccatgtacc tgccagagat tcgcggcttc agcaaggaaa ttgggctggc gttctggcga
     8701 tacattctga ctaacaccac cgttcagtgc gtaacatcgt tcgctgcacg caaattccga
     8761 cacggtcaga tgtactgcgc aatgattggc cttaagcgtg taggaaccat caagaaatac
     8821 ttcaaaggcg tggatgacgt gactttttac agcgccacac gcgaagaact aatcgacttc
     8881 ctgaatcacg ggagatagcc atgttatatg catttaagct gggcagaaaa ctgcgcggcg
     8941 aggaacctta ttgccctgaa aaaggcggga aaggtggcag ttctgataaa agcgcaaagt
     9001 atgcagcaga agcccagaag tatgctgcag acctgcaaaa tcagcagtgg cagacgatca
     9061 tgaaaaacct tgctccgttc acgcctcttg cggagcagta tgttaaccag cttcagaacc
     9121 tttccagttt agaaggtcag gggcaggcac ttaatcagta ttacaactct aagcagtata
     9181 aagaccttgc agggcaggcg cgttaccaga gtcttgctgc tgcggaggcg acgggtggac
     9241 ttggttcgac agccacaagc aatcaactgg ctacgatcgc gccgacactc ggtcagtctt
     9301 ggttatcaaa tcagatgagc aattacaaca atctggcaaa cgttgggctt ggtgcgctgc
     9361 aaggtcaggc aaacgccggg cagacgtacg ccaacaacat gagcagcatt gcacagcaaa
     9421 gcgcagctct tgccgctgct aatgccaata aaccatcaag tcttcagact gcaattagcg
     9481 gtggcacgtc tggtgcgatt gccggtgcag gtcttgccag ccttttggga acatcaacgc
     9541 cttggggcgc tggtattggt gctggtatcg gattgcttgg ctcgttgttt taagggggaa
     9601 tcatggctac ttggcaagga acaaacggcg gattgttggc tggtatcggc ggcgtcaact
     9661 caaacgctcc gagcgtaaat gacatcggca atacgcttca gcttatcagg cagaacaatg
     9721 atattgagcg ttcaggcgct aacaatgttg ggctgactgc tttgcaaggt ctttcaggta
     9781 ttgcgggtgt ttttcagcag gaaaagcagg ctcagcggca gaaagaattt cagcaggcgt
     9841 acgctaatgc ttatgcgtct ggtgatcgcg gtgctttgcg tcagttggct actcaatatc
     9901 cagaccagat tgaatccgtt cgtaaaggca tgggattcat tgatgaagag cagcgtaatt
     9961 ctatcggcac cttagcggct ggcgcacgcc ttgcgtcatc gtctccagaa gcaatgcaat
    10021 catggctgca aaacaacgcc ggtgagttag ctcgtgttgg cgttaatcct catgacgtcg
    10081 ctcagatgta ccaacagaac ccgcggcagt tcggcgaatt tgtcgatcac ctggggatga
    10141 acagtctcgg gcccgaaaaa tactttgacc tacaggataa aatgcagggt cgccaggtta
    10201 ccatgcgcgg tcaggatctg gattcgcaaa ccgccgctcg gaatcaggca atcacaatgc
    10261 gcggacaaga tatccaggcg aatttaggtc agcagcgcat taatctggac gcagaaacaa
    10321 accgcattaa caacgaaaat aagcgccttg accggatgct atcagcagaa actaacgacc
    10381 tgaagcgcca ggaaatacag agccgcatag cagccaacaa ccagcagttg cagcagaagc
    10441 agcaagcgct aaatgatggc tacaaagacg gcatcaacac cctcacaacc agcatgttca
    10501 ctctgaacga tatcgttagt tctccttcac ttaagagcat tacaggctta cgtggagtaa
    10561 tccccaacgt tccaggctca caggctgcag acactcaggc acgacttgat acctttaaat
    10621 cacaagcata cctgacagcg gttcaggcca tgcgaggcat gggcgcactt tctgatgccg
    10681 agggcaaaaa gctcgaccag gctgttggtt cgctgcagaa ctcgcagagc gaggagtcct
    10741 ttcgtcgcaa cgctggcgtc atcctgaaca cgctcaacca gaagcgtaat gaggcggttg
    10801 gtaagtacgt tcagcaaaac ggtatcaagc gagtggaagc gcctcaggct tctatagatt
    10861 acctgaagca gcaccccgag ctgtcaatcg acttcattaa tcgctacgga tatcttccat
    10921 ctttggggca gtaaatggct aattaccgtg atttgttaga gcaggctggc gcacgttacg
    10981 gtgtgccaga agggttgatg actgcactgg gtgccaagga gtcttcttac aaccctgccg
    11041 cagtaagctc cgccggggct gtaggattga ctcaggtcat gcctgggaca tggcgtgata
    11101 tgggttatac cgatgagcaa atgcaaaacc ccgaatatca ggctgacgct ggcgcgcgct
    11161 atctggcaaa gatgtaccag cagtttggta actggcgtga ctctcttcag gcttatcacg
    11221 acggtcccgg caacgttatg aaggcaaagc gtggtgaata tacgccagga cctgaaggcc
    11281 gcggttacgt tgatgatcgc tttgctcaat gggctggtga cccggtgaca gactcaacag
    11341 tcgaacagcg cgccacctct gcaaaggtac atcctcagca agaccctaac aacccgtttg
    11401 cacaactgga agtacagtca tccgaacaag tatcggcatc aggtgtgcag tcagacccaa
    11461 ataatccatt tgctcagatt gagcagcagg cagccagtca gcagccacct caacccgtaa
    11521 gttctgtcgc accgaaacct gttcagcaac aaacagttaa tcaggccaat aatgcaccaa
    11581 cacgggaaga accatcattg atgcaacaag ctggcgattg gctcactggt ggtcaaagtg
    11641 cagggcaaat tgcagaacag gctggtcgtg gtctggtaaa cataccattt gacgtattgc
    11701 agggtggcgc aagtctgatt aatgcaatca gtcaggggct tggggggcca aaagtattgg
    11761 atgatgttta tcgtccagta gacagaccga cagaccccta cgcgcaagct ggagagtcaa
    11821 taggcggtta tcttgttcct ggagcaggtg tagctggaaa catggtcatt ggttctctcg
    11881 ctgacgcggc gaatcaacgg ggtgattttg ccgaaaatgc cgctattaat gccggactta
    11941 gcattgctac gcatggcctg ataaatggcg ttacccgtgg tgttcgtggt gcatcaaata
    12001 taattagtgg caataaaaca tctgcacaga gagcgaccac tgcgccaaca gaaacatcac
    12061 cattctccgg tgatgccgct gcagcaacaa atcctgcggt tcatgccgca gaggcaagag
    12121 tagcacaagg tgtaccaatg acgcctgcga cgaagaaccc agaggaagtc gttcgcacag
    12181 tagcagcaca aaaaaggcca aatctcgctt catcgcttga tgaactagat atcaatcctc
    12241 aggctgaagt tctggagtct gctgaaaggc ttaatgttga ttcattactc ccttcacact
    12301 tttccgggaa cgagcaatac aaggcagttg agcaagcaat caagtcccgt gcgggttctg
    12361 ctctacaggt gcaggaaaat gaagcaatca ggcagctagc acagggcgcg ggtgagataa
    12421 ttgatcgcgt ttccggtgca aaagatgctc ttggtatgag cgacaagttt attgatacgg
    12481 tcaatggaag aatgtctgcg ctgatgaaac gaagcgacca gctttatcgc aatgttgaaa
    12541 aggcgatgcc tgcaggtgca aaaattgatg cgccatcaac aaggtcaatg ctcaaacagg
    12601 tggcagaaga tcttggcggg atgaaaaacc ttgaccctat tgaaaagaga gttgaggtag
    12661 cctgagttta acggacactc cttcctgaaa tagaatggca tcagaaggag ctaataatga
    12721 gcagaaaaac cca
//
LOCUS       DAZXUK010000104        12536 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZXUK010000104 DAZXUK010000000
VERSION     DAZXUK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12536
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.104"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACH3L8_002292"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACH3L8_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFT4085379.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="ACH3L8_002293"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="ACH3L8_002293"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFT4085380.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="ACH3L8_002294"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="ACH3L8_002294"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFT4085381.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="ACH3L8_002295"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="ACH3L8_002295"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFT4085382.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="ACH3L8_002296"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="ACH3L8_002296"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFT4085383.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="ACH3L8_002297"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="ACH3L8_002297"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFT4085384.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="ACH3L8_002298"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="ACH3L8_002298"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFT4085385.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="ACH3L8_002299"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="ACH3L8_002299"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFT4085386.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /gene="hisIE"
                     /locus_tag="ACH3L8_002300"
     CDS             7139..7750
                     /gene="hisIE"
                     /locus_tag="ACH3L8_002300"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFT4085387.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACH3L8_002301"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACH3L8_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFT4085388.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACH3L8_002302"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACH3L8_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACH3L8_002303"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACH3L8_002303"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFT4085389.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..>12536)
                     /locus_tag="ACH3L8_002304"
     CDS             complement(11944..>12536)
                     /locus_tag="ACH3L8_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085390.1"
                     /translation="SIVLTYVLFWFSVTQGDRYLSPIYPLSVLQVAISIASLIKDINI
                     TNYKLGRYFILVCIIFFSGRSLYFEFKYFYSEYLNFNSRLESRYSYELMKKASEYSEL
                     YGDNLIQLGLEDGIYFYKGMAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNS
                     SFIVISKRSYANFNPEKYPKFKVLMDTPNGILIAIK"
BASE COUNT         3201 a         3162 c         3111 g         3062 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggatcaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgagatatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccccg aaaaaaaaat
    12361 aatacacaca aggataaaat atcgtcccaa cttgtaattt gtaatgttga tatcttttat
    12421 taaacttgca attgagatcg caacctgcaa tactgacaat gggtaaatgg gtgataaata
    12481 cctgtcacct tgcgtaacag aaaaccaaaa taagacatat gttaaaacaa tcgaga
//
LOCUS       DAZXUK010000105        12469 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZXUK010000105 DAZXUK010000000
VERSION     DAZXUK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12469
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.105"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1466)
                     /gene="emrY"
                     /locus_tag="ACH3L8_002305"
     CDS             complement(<1..1466)
                     /gene="emrY"
                     /locus_tag="ACH3L8_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFT4085391.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAA"
     gene            complement(1466..2629)
                     /gene="emrK"
                     /locus_tag="ACH3L8_002306"
     CDS             complement(1466..2629)
                     /gene="emrK"
                     /locus_tag="ACH3L8_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFT4085392.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            3045..3659
                     /gene="evgA"
                     /locus_tag="ACH3L8_002307"
     CDS             3045..3659
                     /gene="evgA"
                     /locus_tag="ACH3L8_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFT4085393.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            3664..7257
                     /gene="evgS"
                     /locus_tag="ACH3L8_002308"
     CDS             3664..7257
                     /gene="evgS"
                     /locus_tag="ACH3L8_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFT4085394.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(7313..8458)
                     /gene="yfdE"
                     /locus_tag="ACH3L8_002309"
     CDS             complement(7313..8458)
                     /gene="yfdE"
                     /locus_tag="ACH3L8_002309"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFT4085395.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(8532..9476)
                     /gene="yfdV"
                     /locus_tag="ACH3L8_002310"
     CDS             complement(8532..9476)
                     /gene="yfdV"
                     /locus_tag="ACH3L8_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFT4085396.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(9546..11240)
                     /gene="oxc"
                     /locus_tag="ACH3L8_002311"
     CDS             complement(9546..11240)
                     /gene="oxc"
                     /locus_tag="ACH3L8_002311"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFT4085397.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(11294..>12469)
                     /gene="frc"
                     /locus_tag="ACH3L8_002312"
     CDS             complement(11294..>12469)
                     /gene="frc"
                     /locus_tag="ACH3L8_002312"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFT4085398.1"
                     /translation="LAWFGADVIKIERPGVGDVTRHQLRDIPDIDALYFTMLNSNKRS
                     IELNTKTAEGKEVMEKLIREADILVENFHPGAIDHMGFTWEHIQEINPRLIFGSIKGF
                     DECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLVSAAALGDSNTGMHLLIGLLAALL
                     HREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDKLGYLEEYPQYPNGTFGDAVPRGG
                     NAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWENTCKAIGKPEWITDPAYSTAHAR
                     QPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCAPVLSMKEISLDPSLRQSGSVVEV
                     EQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         3717 a         2795 c         2540 g         3417 t
ORIGIN      
        1 gccgccatac ggaaaatctc atttgcagaa atagaaagtg actgctgggt tatttcatta
       61 ttaatttcat taagaactcc tgaaagcgaa ccatagtatt tatccataat ttgtgacgat
      121 gaattaaaca cggggttaaa ttgatcgatg gttgctgtca actgactatg gtgtaacgat
      181 tcgcgtcgtc cccacagcgt cattgtcaac gacgtaccaa ctgatcctga caaggtacga
      241 aaaaaattac tcatactcga ggcattggca aatttattat ctggcaagcc tgaaaacgaa
      301 atcgttgtta agggtaaaaa gaaacaggca acggcgaatc cctggaaaaa ctgtggcaaa
      361 atgatgcctg taaaatcaat cgttggcata aatgtcacag aacgccagta atagcaaacc
      421 gcatacatca aaaaactgaa tgtcactaac acccgcatgt ctattttatt gccataacgt
      481 cctatcaaag gtgaaatcaa tagtggcatg atgccgatgg gcgcataagc aagcccggcc
      541 catatcgcat tataccccat cgttttctgg agtaactgcg gcataaggac gatcgctcca
      601 gagtaaaata aatacgcgca ggtgatactc acaataccaa tggtgaaatt acgggactta
      661 aacaaactga gatcaagaat cgggttctct gaggtcgatt cccaaatgac taaagagatc
      721 agagaaataa ctgatactac tgttaatatt attattgtac tcgagttgaa ccaatccaga
      781 tcgcgccctt tatcaagcat aatttgcaag ccaccaacac cgagcactaa cagggtcagt
      841 cttggtagat tcatttttac cggtgaagtt tcagtttctc ttcctttaag taaggttaag
      901 cataatgtca ggacgataat ccccataggg acattgatta aaaatatcca accccagcta
      961 aagttatcgc aaatatagcc gcccaatatc ggcccacata tcggagcgat aatcacggtc
     1021 attgaccata atgccagagc aaatgttctt ttttctggtg gataattcct taataataaa
     1081 ctctgtgaca gtggaattaa cggccccgcc attaatccct gaacgactct aaaaaatatc
     1141 agcacatcaa gattggtcga taaactacac attaaggaag acagcgaaaa aaaagtgact
     1201 gaaagtaaaa ataaccttaa ttcgcctatt ctttgtgcca gcctgccggt aacagggatc
     1261 gcaatggcat ttgctacgcc aaacgaggtg ataacccagg tgccttcgtc tgttgatgct
     1321 cccagaaagc cagatattgt cggtattgcg acgttagaaa tagtggaatc caacatttgc
     1381 ataaacgtcg ctaatgacaa tgcaatagta acgcaccata acgtcccacc ggttaatggt
     1441 gccggagttg atttagtgat tgccattaaa gttgtccatt atgcgaaata atgttgctaa
     1501 tttctttttc tatcggactg gtatcgataa ctaaagcctt actggtataa gcaggcatgg
     1561 aggtcacggt tgaagccaga tcaggcatct cagcaatgtc ttcgtccttc gtatcaatag
     1621 ttgcagtcat cgataaacca atgcgcaagg ggtgttccat gagttctttt ggatcaagag
     1681 aaacttcaac cggtacacgc tgaacgattt tgatccagtt ccctgtcgca ttttgtgcag
     1741 gtaataagga gaacgcattg ccggttccca tattgatccc tgtcacccga ccatgaaaca
     1801 caacattttc accataaaga tcgctgataa tattgactga ttgaccaatc cgtacatccg
     1861 tgagttgtgt ttctttaaag ttggcattaa cccacatttg acgtgccggt acgacagcca
     1921 ttaacgattg tccggaactc actgtttcgc cgacctgaac acttcgctgg gcaatatagc
     1981 cagtaaccgg actccttata tccgtacgtt taagcaccaa ccaggcttct ttggttgcat
     2041 ccgccgcttc aactacttgt ggctgacggt ttaatggtgt gttcattact aaagctttat
     2101 tcgctttata agcctggata gcggcattca gtgccgcttt gctacttatt aacgtatctt
     2161 tggtatgctc cagcgtttct tttgaaataa ccccctgctt cgctaaaggc acacgacggt
     2221 tataatcttc taaagattgt tgatactgaa tacgtgctga agcgacttcg gcactgtatt
     2281 gtttatcctg taagtacagt ttattcgttt gccgaacaat atttgccaga ttatttttag
     2341 ctttattgag tgcgatagtg gcatcagttt tatccagtga aactaaaatg tcaccctgtc
     2401 gaacgtagtt cgtatcttta tgattaacta cagtgacact acctgagact tgtgcagaaa
     2461 ttggatctgc attccccgtg acataggcgt catctgtact aatcatgtct tctaattcca
     2521 ttgaccaata ggcataggca cctgaaaacg caataaataa aactatcacc aataaagaaa
     2581 agtatttttt tctgttagac tgttttttat ttgaattaat ctgttccact attatctctc
     2641 atttcgcata gatgaaattt atgaattgaa tgaaaagagt attcattctt catatcaaac
     2701 acagtgcatg tgttgcacat tatcccagga acgttggagg cctaatttaa atgaggtgca
     2761 aaaaaaacaa cagaggacta aaccgtggct tttgcaatac aattcttacg cctgtaggat
     2821 tagtgagaag acttatagtg ctaacttgaa actataaatc atcggtataa tccctgattt
     2881 tattgttgac atttcattta tgccgactat ttatatggta tacttgtcga attatcttaa
     2941 aggaaactca gattttctta tttttattga gaaaatgaga tgacgcctta tgtctgtatt
     3001 actacaggga gaagggagat gcttcattgc aaagggaata atctatgaac gcaataatta
     3061 ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt attgatcaaa aacgatattg
     3121 aaatcttagc ggagttaact gaaggcggaa gcgccgttca gcgggtggaa acacttaagc
     3181 ctgatatcgt catcattgat gtcgatatcc ccggagttaa cggtatccag gtgttagaaa
     3241 cgctgaggaa gcgccaatat agcggaatta ttattatcgt ctccgctaaa aatgaccatt
     3301 tttacgggaa acattgtgct gatgctggcg ctaatggttt cgtgagtaaa aaagaaggca
     3361 tgaacaatat cattgcggct attgaagctg caaaaaatgg ctactgctat ttccccttct
     3421 ctctcaaccg gtttgttgga agtttaacgt ccgaccagca aaaactcgac tccttatcga
     3481 aacaagaaat tagtgtcatg cggtatattc ttgatggcaa ggataataat gacattgcgg
     3541 aaaaaatgtt catcagcaac aaaactgtca gcacgtataa aagtcgcctg atggaaaaat
     3601 tagaatgtaa atcactgatg gatctttaca cattcgcgca acgtaacaaa atcggctaat
     3661 cacatgaagt ttttacccta tttttttctt ctctgttgtg gtctttggtc gaccataagt
     3721 ttcgcagacg aagattacat cgaatatcgt ggcatcagta gtaacaaccg tgtcacactt
     3781 gatccagtac gtctgagcaa caaggaatta cgttggttag cgagcaaaaa aaatcttgtg
     3841 attgcagtac acaagtccca aacggctacc ttgttgcata ccgattcgca gcaacaggtt
     3901 cgtggtatta atgctgatta tttaaatctt ttaaaaagag cgttaaatat caaattaaca
     3961 ctccgggaat acgcagatca tcaaaaagca atggacgcgc ttgcagaagg tgaagtcgat
     4021 atagtgttat cacatttagt tacttcgccg cctcttaata atgacattgc tgcaaccaaa
     4081 ccattgataa ttacctttcc ggcgctggta accacccttc acgactcaat gcgaccgctt
     4141 acctcaccaa aaccagtaaa tattgctcgg gtagcaaatt accccccaga cgaggtaatt
     4201 catcaatcat ttccaaaagc aacaattatc tcttttacaa atttatatca ggcattagca
     4261 tccgtctcag ctgggcataa tgattacttt attggtagta acatcattac cagcagtatg
     4321 atttcccgct atttcactca ctccttaaat gtagtgaaat attataactc gccgcgtcaa
     4381 tataattttt tcttgaccag aaaagaatct gtcattctta atgaagtact caatagattt
     4441 gttgatgctt taacaaatga agttcgctat gaagtatcac aaaattggct tgatacagga
     4501 aacctggcct ttctgaacaa accattagaa ctcactgaac atgaaaaaca gtggattaag
     4561 cagcatccca atttaaaggt gctggaaaat ccttactcgc ccccctattc tatgacggat
     4621 gaaaatggct cggttcgggg cgttatgggg gacattctta atattattac cttgcaaaca
     4681 ggtttaaatt tttctccgat caccgtttca cacaatatcc atgctggaac acaacttagc
     4741 cccggaggat gggatataat acctggcgct atttatagtg aagatcgaga aaataatgtt
     4801 ttatttgctg aagccttcat aacaacgcct tacgtttttg tcatgcaaaa agcgcctgac
     4861 agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc catattatta tgagctgcat
     4921 tcgcaattaa aagagatgta tccggaggtt gaatggatac aggtcgataa tgccagcgct
     4981 gcatttcaca agattaagga aggtgaactt gatgctctgg tcgcgacaca gctaaattcg
     5041 cgttacatga tcgatcatta ctatcctaat gaactttatc attttcttat tcctggcgtt
     5101 ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac cggaacttaa ggatattatt
     5161 aataaagcac tgaatgcaat tcccccaagc gaagttctgc gcctgacgga aaaatggatt
     5221 aaaatgccca atgtgaccat tgacacatgg gacctatata gcgagcaatt ttatattgtt
     5281 acgacattat ccgttttatt agttggcagt agccttttat ggggattcta cctgttacgc
     5341 tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa accaaatatc attccgaaaa
     5401 gcactctcgg attccttacc gaatccaact tatgttgtaa actggcaagg taatgtcatt
     5461 agtcataata gtgcttttga acattatttc actgcggatt actacaaaaa tgcaatgtta
     5521 ccattagaaa acagtgattc accctttaaa gatgtttttt ctaatgcgca tgaagtcaca
     5581 gcagaaacga aagaaaatcg aacaatatac acacaggtat ttgaaattga taatggcatc
     5641 gagaaaagat gcattaatca ctggcataca ttatgcaatc ttcctgcaag tgacaatgca
     5701 gtatatattt gtggttggca agatattact gaaacgcgtg atctaattaa tgcactcgag
     5761 gtagaaaaaa ataaagcgat aaaggctacc gtagcaaaaa gtcagtttct ggcaacgatg
     5821 agtcacgaaa taagaacacc aataagctct attatgggct tcctggaact tctgtcgggt
     5881 tctggtctta gcaaggagca acgggtggag gcgatttcac ttgcctacgc caccggacaa
     5941 tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc gggtaactat
     6001 caacttcaac cacaatgggt cgatatccct actttagtcc agaacacttg tcactctttc
     6061 ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca gcagtacatt tcctgatcat
     6121 tacctggtta agatcgaccc tcaggcgttt aagcaggtct tatcaaattt actgagtaat
     6181 gctctcaaat ttaccaccga gggggcagta aaaattacga cctccctggg tcacattgat
     6241 gacaaccacg ctgttatcaa aatgacgatt atggattctg gaagtggatt atcgcaggaa
     6301 gaacaacaac aactgtttaa gcgctacagc caaacaagtg caggtcgtca gcaaacaggt
     6361 tctggtttag gcttaatgat ctgcaaagaa ttaattaaaa atatgcaggg cgatttatca
     6421 ttagaaagtc atccaggcat aggaacaaca tttacgatca caatcccggt agaaattagc
     6481 cagcaagtgg cgactgtcga ggcaaaagca gaacaaccca tcacactacc tgaaaagttg
     6541 agcatattaa tcgcggatga tcatccgacc aacaggctat tactcaaacg ccagctaaat
     6601 ctattaggat atgatgttga tgaagccact gatggtgtgc aagcgctaca caaagtcagt
     6661 atgcaacatt atgatctgct tattactgac gttaatatgc cgaatatgga tggttttgag
     6721 ttgactcgca aactccgtga gcaaaattct tccttaccca tctgggggct tacagccaac
     6781 gcacaggcta acgaacgtga aaaagggtta agttgcggca tgaacttatg tttgttcaaa
     6841 ccgttgaccc tggatgtact gaaaacacat ttaagtcagt tacaccaggt tgcgcatatt
     6901 gcacctcagt atcgccacct tgatatcgaa gccctgaaaa ataatacggc gaatgatcta
     6961 caactgatgc aggagattct catgactttc cagcatgaaa cgcataaaga tctacccgct
     7021 gcgtttcatg cactagaagc tggcgataac agaactttcc atcagtgtat tcatcgcatc
     7081 cacggtgcgg ctaacatcct gaatttgcaa aagttgatta atattagcca tcagttagaa
     7141 ataacacctg cttcagatga cagtaagcct gaaattcttc agctgctgaa ctctgttaaa
     7201 gaacacattg cagagctgga ccaggagatt gctgttttct gtcagaaaaa tgactaaatt
     7261 gcggctccca caatgttcta atgtgggagc tatttaccag cacatcttac atttatgatg
     7321 agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc ggcataacat
     7381 gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt cctccggctt
     7441 caatcaacat atttctcgcc tgagtttgtg gcaaattaat ggcctcagcc acacttaata
     7501 acggcgcgac gggcacacca acttcatgta ttttggctaa ccaaacttca gctgcctgcg
     7561 tttttaacgt tcgctcaata tattgtttaa gaatagcctg gttttgtacg cgtaaaatat
     7621 tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc cagtgcctgg cataacgcag
     7681 aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta tcgaaaacat
     7741 caaaaggtgc catgtaggga tggcgatttc ccaggcgttg tggtgacttc cctgtcgcaa
     7801 tatatgccat cagaccatgc tccagaaaac tcagcgtggc atcaaacatc gctatatcga
     7861 catgcgcccc tctctggctc ttttcgcggc cataaagtgc actcactatt ccgctgaata
     7921 aatagacacc gccgcataga tccgcaagag aggtaccaac acgcactggc ggagcatcag
     7981 ggtaccctgt ttccatcatt atcccactca ttgcctgaat gatggtatcg taggcaggag
     8041 catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatatg aggcgcgggt
     8101 tgatttcttg taacctttcc catgaaaacc ccagtttttc cattgtacct aggcgaaaat
     8161 tctcagctaa tacatcagct tgtttgagca tatttataaa tatactttta tcgtgatcat
     8221 tctttaaatc aagaaccaca ctctctttgc catgattaat aaaactgtaa tagagtgact
     8281 gtccatccac atagggacca aatgtgcggg tatcatcacc atgacctggt ggctcaactt
     8341 taattaccct tgcgcccata ttataaagaa gttgagttcc gaaaggtcca ttaaggacat
     8401 gtgtcatatc gataactaat aagccttcaa acggcccttt gctttcatta tttgtcattt
     8461 gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc ggcatttatg taaaatcaat
     8521 aacatgatta attatgaaac cagtcgactg acataaatcc acaagggagc cgtgacgaca
     8581 aagcccagta cgctcacagc caatgacgct gtaccagtac gggtgtagac attaaaccgg
     8641 ctggcaataa tgattccgga gaatgccggc ggtaatgcgc ctgccagtac catcatctgc
     8701 aaatgttcgc tgttcaaatg acatgccata ccaacgagaa gcagcgccag tggcatcaga
     8761 atcaacttca ggaaggtgtt ataagcaatt tcggcactga actcgaattt atgtgcagcc
     8821 agagtcaacc ccgcagcgaa taccgctacc cctgagttag ccttcgcaat cagattaaag
     8881 gttgggtccc atgctgccgg aatttttacc ccaaccagca ccaggatcgt tgccagaaca
     8941 ggtgcccata ctactggctc ctttgccgca gaaattaatg cgctcagatt actattcttc
     9001 ttaccatccg ctcctgagga aggattcagc aaatacagac caataggaat agtaattgcg
     9061 ttaacaataa tagaaataat tgctaccact aaacctgttg atacggaatc accataaata
     9121 ggatcgagaa ctgcaaaccc caggaatcca atggtaggtg aacccgcaat taatgcacat
     9181 acagctgctt ctgcatgggt acgtttaaaa aatttgtagc aaccaaacca ggagaaaaag
     9241 aaacatccga caataaccac aagtgataca agggtcagac gagtgtccgc aaaaatcatt
     9301 tccctgtttg cccgggtaat agaaacaaat agagccgcag gaagagcgta gtttaatacc
     9361 agtttattaa atgcgcgggc ttgatcttct gaaaaagttt ctcgtctgcc gctaaaataa
     9421 cccaacagca tgattacgat aataggcaat aaatcgccaa taaaaaatgt tagcataata
     9481 gtatttccga taagggtccc ctcaccatcg cataatgagt tggcaagagg agacgatgtc
     9541 agtatttaat taccagcgac ttgttttggg ttaagtttgg taatatggcc actttcagtt
     9601 cctgctgcag ggtcgataac cacattaata atggtcggtt tgcgcgactg aataccggtg
     9661 gttaaagcat gacgaagttc atctgtcgtg gtgacgttat agccaacgcc acgaaacgca
     9721 tccattaatt tgtcatacct tgcatggtgc aacagatccg ttggtgatgg tgcaccagcg
     9781 ccactgagat caacaccgtc tcctctgtag atgccgccat tattaaaaat aacgatcgtc
     9841 accggcaggt tatatcgaca aatcgtttca atttccatcc cactgaaacc aaaagcacta
     9901 tcaccttcaa tggcgacaac cggagaacca gaagtcacgc tagcaccgat ggcatagccc
     9961 ataccgatgc ccatgacacc ccaggtacca caatccagac gacgacgtgg tttatacata
    10021 tcaataatat ttcgtgcatt atccagggta tttgcacctt cattaactaa ataaatatcc
    10081 tggttctcgc gcaatacgtc gcgcacagca cttaatgcat taaagtaatt taatggttga
    10141 gtatctgtac ttaatttttc atgcattttt tgtgcatttt gctgcttgtg gatatttaaa
    10201 atatcgcgcc ataccagtgg agtcgtaaat gtgttttgtt tcagttctgc cagcatacct
    10261 tgcatactgg atgcaatatc accaacgact ggcacagcaa tggggcggtt gctgtcaatt
    10321 tcctgcggtt caatatccag ttgaataaac tgtgtatctg ccgcccatcc ttttttaccg
    10381 tgtgccaata accaattcag tcgtgcacca acaagcatga caacgtcagc atttgccagg
    10441 gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat cttcaaggat ccctttcgcc
    10501 atagacattg gcaggaatgg aatctgagca ctttcaataa attcacgaag ctgttcatca
    10561 gcttgtgaat acgccgcgcc tttgccaagg ataattaatg gccgttcagc ttttgctaaa
    10621 agcgaaattg cgctagtgac tgacttcggg catggcaata atgctggcga cggattttca
    10681 actttaacaa tcgtggttaa cgcttcgtct ttttccatcg tcgcggccag gacatttgct
    10741 ggcaaatcaa gataaactcc gccagggcga cccgatacag agacccggat agcgcgtgcc
    10801 aatgcaatgc caagatcctg cggctgatta acgcgaaatg ctgctttggc atacggtttt
    10861 gccgcattca tttggtccag ctcttcataa tcaccttgct gtaggtcgac gatcgcgcgg
    10921 tcgctggagc cgctaatcat gataatcgga aaaccattta ccgttgcgtt ggccaatgcg
    10981 gtcaaaccat tgaggaatcc tggcgcagaa actgtcaggc agatccccgg tttttgggta
    11041 agaaaaccgc ttgccgcagc ggcatagcct gccgactgct catgacgaaa accaatataa
    11101 cgaatgcctt ccgcctgggc atggcgtgcc atatccgtca caggaatacc tacaacacca
    11161 taaatagtgt caatattatt ctgttttaat gcttcaacga tgatatgcat accatctgtc
    11221 atttgaagtt gatctgacat gattaataac ctcgaaattt ttataaattg ggagccagaa
    11281 gcccccgtta atatcagatg gcgtggtttt gcttcattgc agcaatttca tcgtcgctat
    11341 aacccagctc ctgcaataca gcagcggtat gttcacctaa tagcggcgca gctttaatat
    11401 ccggcgtaaa ggcagagaat ttcattggac aaccaacggt cagatatttt ccacgcaacg
    11461 gttgttccac ttcaacaaca ctgccacttt ggcgcaaaga gggatcaagt gaaatttctt
    11521 tcatacttaa aaccggtgca caaggaatat caaactgagt caaataggcc accgcttcat
    11581 gtttatcaat agtgacagtg tatttttcga tttcagcaaa aatatcgaaa atatgtggct
    11641 gtcgggcatg ggctgtactg tatgccggat cggtaatcca ttcaggtttg ccgatggctt
    11701 tacaggtgtt ttcccagttt tgctcctgaa tagtgaaata aatataggcg ttaggatcgg
    11761 tttcccagcc tttacatttc aggatccagc caggctgacc gccaccacct gcattaccac
    11821 cgcggggaac tgcatcacca aatgtaccat tcggatactg cgggtattct tccagataac
    11881 ccaatttatc gagacgctgc tggtcacgta atttcacgcg gcaaaggttc aatacggcat
    11941 cctgcattga catggtgact cgttgcccac gccccgtttt ttcgcgatgc agcaaagcag
    12001 caagtaaacc gatcagcaaa tgcattccgg tgttgctgtc acccaacgct gcagcgctta
    12061 ccagcggcgg accatcccaa aaacccgtag tggatgccgc gccacccgct gcctgagcaa
    12121 cgttttcata ggcttttaca ttcacataag gcgaacactc atcaaaccct ttgatcgaac
    12181 caaaaatcag acgtggattg atttcttgaa tatgctccca ggtgaagccc atgtgatcaa
    12241 tggcccctgg atgaaagttc tcgactaaga tatcagcttc gcggatcagc ttttccatta
    12301 cctctttgcc ttccgctgtt ttggtattta actcaataga acgtttgtta ctgttaagca
    12361 tggtgaagta aagcgcatcg atatcaggaa tatctcgcag ttggtgacgc gttacgtcac
    12421 caacgccggg acgttcaatt ttaataacgt cagcgccaaa ccaggccag
//
LOCUS       DAZXUK010000106        12449 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZXUK010000106 DAZXUK010000000
VERSION     DAZXUK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12449
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.106"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..273)
                     /locus_tag="ACH3L8_002313"
     CDS             complement(<1..273)
                     /locus_tag="ACH3L8_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFT4085399.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
     gene            complement(286..2067)
                     /gene="gcl"
                     /locus_tag="ACH3L8_002314"
     CDS             complement(286..2067)
                     /gene="gcl"
                     /locus_tag="ACH3L8_002314"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFT4085400.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2157..2972)
                     /gene="allR"
                     /locus_tag="ACH3L8_002315"
     CDS             complement(2157..2972)
                     /gene="allR"
                     /locus_tag="ACH3L8_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFT4085401.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3050..3532)
                     /gene="allA"
                     /locus_tag="ACH3L8_002316"
     CDS             complement(3050..3532)
                     /gene="allA"
                     /locus_tag="ACH3L8_002316"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFT4085402.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3762..4688
                     /gene="allS"
                     /locus_tag="ACH3L8_002317"
     CDS             3762..4688
                     /gene="allS"
                     /locus_tag="ACH3L8_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFT4085403.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            4757..5851
                     /gene="mnmH"
                     /locus_tag="ACH3L8_002318"
     CDS             4757..5851
                     /gene="mnmH"
                     /locus_tag="ACH3L8_002318"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFT4085404.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            6263..6346
                     /gene="dinQ"
                     /locus_tag="ACH3L8_002319"
     CDS             6263..6346
                     /gene="dinQ"
                     /locus_tag="ACH3L8_002319"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFT4085405.1"
                     /translation="MKTFLDKVLIVLKALIALLELIRQFIE"
     gene            complement(6564..8979)
                     /gene="ybbP"
                     /locus_tag="ACH3L8_002320"
                     /pseudo
     CDS             complement(6564..8979)
                     /gene="ybbP"
                     /locus_tag="ACH3L8_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
     gene            complement(8976..9662)
                     /gene="ybbA"
                     /locus_tag="ACH3L8_002321"
     CDS             complement(8976..9662)
                     /gene="ybbA"
                     /locus_tag="ACH3L8_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFT4085406.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            9633..10255
                     /gene="tesA"
                     /locus_tag="ACH3L8_002322"
                     /pseudo
     CDS             9633..10255
                     /gene="tesA"
                     /locus_tag="ACH3L8_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            10245..11054
                     /gene="ybbO"
                     /locus_tag="ACH3L8_002323"
     CDS             10245..11054
                     /gene="ybbO"
                     /locus_tag="ACH3L8_002323"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFT4085407.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            11115..11969
                     /gene="cnoX"
                     /locus_tag="ACH3L8_002324"
     CDS             11115..11969
                     /gene="cnoX"
                     /locus_tag="ACH3L8_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFT4085408.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(12032..>12449)
                     /gene="fetB"
                     /locus_tag="ACH3L8_002325"
     CDS             complement(12032..>12449)
                     /gene="fetB"
                     /locus_tag="ACH3L8_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFT4085409.1"
                     /translation="QVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKLSLGAT
                     PKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQIMVTF
                     MLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
BASE COUNT         3058 a         3447 c         3161 g         2783 t
ORIGIN      
        1 aatcgctgct gctacgccat cccgaaactc ttcttcacgg ccaggaatac aggcaatacc
       61 gcgctcgccc gccgcccagt cacctgccgg taaattgtgc agcgtatgtt cgagtttatt
      121 actcgccagt acctgtttta attcttcaat gtcgtagtca taaggaaaca taaattcaac
      181 gccgcgaaaa ccacactgcg cagctttctc aaaacgggcg agaaaatcat attcaccaaa
      241 taacatcgat aaattagcag agaaacgtaa catcatttat ctcccttatt catagtgcat
      301 gaagcaggtt tcagtcggtg catccgctgc gttatcggcg atatcttcaa attccatgac
      361 gttatccagt tcgctgccca tcgaaatatt ggtcacacgc tcgagaataa cttccacgac
      421 taccggtacc cgatattgcg ccattaaggc tttcgcctgt tcaaaggctg gcgcaatatc
      481 ttccggtttg aagacccgaa tagctttaca acctaaacct tccgctactt ttacgtggtc
      541 aacaccgtag ccattcactt cactggagtt gatattctcg aaagcgagtt gcacgcagta
      601 gtccatgtca aaagcgcgtt gtgactgacg aatcaggccg agataggcgt tattcaccag
      661 cacatggatg tacggaatgt tgaactgcgc gccaacggct aactcttcaa tcaggaactg
      721 gaagtcaaag tcgccagaaa tcgccaccac attgcgtttc ggatcagcgg cacaaccccc
      781 cagcgccgcc ggaatcgtcc agcctaacgg accagcctga ccacagttga tccagtggcg
      841 gtctttaaag acatgcagca tttgtgccgc agcgatttgt gacagaccaa tagtggtgac
      901 ataacaaaca tcgcgaccaa aggctttgtt catctcttca tacacgcgct gcggtttcac
      961 cggcacgttg tcgaagtggg ttttgcgcag caaagtgcgt ttacgctgct ggcagtcggc
     1021 gacccattct ttacgacacg gcagacgacc cgctttttgc atctcctgcg ccacttcaac
     1081 cagcagtgtc agcgccgctt tagcatcaga gacaatgccg agatccggac acagcacgcg
     1141 accaatttgc gtcggctcaa tatcaatatg aacgattttg cgcccttcgg tgtatttctc
     1201 taccgaaccg gtatgacggt tagcaaaacg gttaccgata ccaaacacca tgtcggacgc
     1261 cagcaacgtt gcgttaccgt aacgatgcgc ggtttgcaga ccgaccatcc cggccatcag
     1321 ttcatgatcg tccgggatac agccccagcc catcagcgtt gggatcaccg gaacgctggt
     1381 cagttcagca aactgttgta acagtgcagc tgcgtcagca ttgattaccc cgcccccggc
     1441 aacaatcacc ggacgttcgg cctggattaa catttctaca gctttttcga tctgcatacg
     1501 gctggcagca ggtttgtaga ccggcagcgg ttcgtacatg tcaggatcaa actcgatttc
     1561 cgcaacctga acgtcgaacg gtaaatccac cagtaccgga cccggacgac cagaacgcat
     1621 caggtgaaat gcctgttgca gcacgcgagg caccagcgcc gcttcacgaa ctgtaaccgc
     1681 cattttgctg accggtttag caattgcttc aatatctacg gcctgaaaat cttctttatg
     1741 cagacgggcg cgcggtgcct ggccggtaat gcacagaata ggaatggaat cagcagaagc
     1801 ggaatagagc gcggtgatca tgtccgtgcc cgcaggaccg gaagtcccca gacatacgcc
     1861 gatattccct gccgttgcgc gggtataacc ttccgccatg tgcgaaacac cttccacatg
     1921 acgcgccaga atgtgacgaa taccgccgtg cttacgcatc gctgagtaga acggattaat
     1981 tgcagctccc ggaacgccga aggcggtggt gataccttct ttctccagca catacattgc
     2041 cgcgtcaacg gctctcattt ttgccatttt attcctacct ctattagttg gaaaaatttt
     2101 ccaactttca aattttgtca aaaaaacccg ctccgcagag cgggatgcga cagacactat
     2161 ggatgtgctt tcagtcccaa cgccgtgctg atatcgcggg cggtgtctct gaccagctca
     2221 ccctgactga caaaacgatc ttctgtcagt cttgatgaag gcccggagat ggagatagcg
     2281 gcaacaactc taccgacatc atcgtaaatt gctgaagcta tgcaattcag acctacaaca
     2341 tgctcttctt tatctacggt atagcccagt tcacgcgctt gttccaggtc cttcagcaag
     2401 gtgggcatat ccacaagcgt agttggcgta aactgctgca aaccggtttg cagaatgatg
     2461 ctcatcaact cctcttccgc cagcggataa agcagcgctt tgcccgcacc ggaagcatgc
     2521 agcggcagac gactgcccag tggcgcacac atcctgacca tcgatttaca ctctaactga
     2581 ccaattaata ccgcttcatt gccgttacgg atcgcgacat tgaccgtttc gccggaaagt
     2641 aacatcaggc ggcgcataaa cggcccggcg acggagagga catcgcggtt atggatgtac
     2701 gccgcaccga cgttaaagac acctaatcct atatgccacc agcctaattg actgtcctga
     2761 tagacaaaat ccgctgcttg taaaaccttc agcaagcgaa aggtcgtgga gagcggcaaa
     2821 tccagattga gagaaatatc gctaaccgac gaacttcccc cacttttttc cagatattgc
     2881 agaatcgcaa ttccccgctc taacgcctgt gcgcccttct gtgccacagg ctccgcctgt
     2941 cctggcctgc cgcgccgtct aacttccgtc atacctacgt taactccgac ctacaagtga
     3001 gcataaacaa tgaacaatta gtttatctta tgcaagtcgg ttgcaggcgt cacgcaaaac
     3061 agagttcctg ttcaggaata ctttcaacat cacagttgtc actgccgccg cgatcgatgg
     3121 tcagaaaatc ggtgacgcgc tgccaggcga aaagtgggtg atgccagacg ttacgatggt
     3181 aattcacccc ctgttcaccg ttggtgataa acgcccgcag cgttgacagg tctggtttgt
     3241 cgtcgcctaa cgccacgacc accacaaaca cttcaccttt cattgggata aaggcctgag
     3301 tgcccagcgg atgacgttcg agttcgtgaa tggtcagcgg cagattcgcc ggttgcgcgc
     3361 ggttaatgct gataagcgta cggtcttgct caagaatctc aaccagcgcc aaatcgtggt
     3421 aacgctccac caggccattg ttaatatgga aaaaatcccg ttgctgcgtt tcgattacgt
     3481 cgccataagc actaaaggct tcctgactta acggtaatac ctgaagtttc atctttttgt
     3541 ccttgtaaca taattccgtt caaccaccgc cgaccggtca gcttggtttc aataacaggc
     3601 aatctaaaac cttaagagaa caacattaat acaaaagttg gaatgttttt ctaaaactag
     3661 atttagtcca caaattgaac aggattatca tgtaaaaata ctgataaatc agaggataag
     3721 aaaatcacgc aacttgcgga tttaaaaggg gatacctgaa gatgttcgat ccagaaacct
     3781 tgcggacttt cattgcggtt gctgaaacag gaagtttttc aaaagcggca gaacgattat
     3841 gtaaaaccac ggcgacgatc agttatcgca ttaaacttct ggaagagaat accggagtag
     3901 cgctgttttt ccgtacgact cgtagcgtaa cgttgacagc ggctggcgag catctacttt
     3961 cccaggccag agactggctg agctggctgg aaagtatgcc cagcgagctg caacaggtga
     4021 atgatggcgt ggaacgccag gtgaatattg tcatcaacaa cctgctctat aacccccagg
     4081 ccgtcgccca gttgctggcg tggctgaatg agcgttaccc ctttacccag tttcacatct
     4141 cccgacaaat ctatatgggc gtctgggact cgctattgta cgaaggtttt tcgctggcta
     4201 tcggggtcac gggaactgag gcgctggcaa atacctttag tcttgatccc ttaggatcgg
     4261 tgcaatggcg atttgtcatg gcggcggatc atccgctggc gaacgttgaa gagccgctaa
     4321 cagaagcgca gttgcgtcgc tttccggcgg tcaatattga agacagcgcc ggcaccttaa
     4381 ccaaacgcgt cgcctggcga ttgccggggc aaaaagagat tattgttccc gatatggaaa
     4441 cgaaaatcgc cgcccatctg gcgggtgttg gcattggttt tttgccaaaa tcactttgcc
     4501 agtcaatgat cgataatcaa caactggtca gccgggtaat cccaacgatg cgccctcctt
     4561 cgccattgag tctggcatgg cgcaaatttg gcagctgcaa agcggtagaa gatattgtga
     4621 ccttgtttac ccaacacagg ccggaaatca gcggattttt agaaattttc ggcaccccac
     4681 gcagttaaaa atcaagtttc cttgcgcgtt ggcgcgtaaa atagcgcgca agctgatttg
     4741 aggttcactt ttaactatgc aagagagaca cacggaacag gactatcgtg ccctgctgat
     4801 tgctgatacg cccattattg atgttcgcgc ccctatcgag tttgagcaag gcgcaatgcc
     4861 cgccgctatc aatctgccgt taatggataa cgatgaacgc gcctccgttg gcacctgcta
     4921 taaacagcaa ggctcagacg cagcgctggc gctggggcat aaactggtgg cgggtgaaat
     4981 tcgtcagcag cgtatgtacg cctggcgggc agcgtgcctg caaaatccgc aaggtattct
     5041 ctgctgcgcc cgtggcggtc aacgttcaca tattgtgcaa cgctggctgc atgaagcggg
     5101 gattgattat ccgctggtgg aaggcggtta taaggcactg cgccagaccg cgattcaggc
     5161 gaccattgaa ctggcacaaa aaccgatagt gctgattggc ggttgtaccg gcagcggtaa
     5221 aacgctatta gtgcaacaac agccaaacgg tgttgatctg gaagggctgg cgcgtcatcg
     5281 cggttcggcg tttggtcgca cgttacaacc acaacttagc caggcgagtt ttgaaaacct
     5341 gctggctgcc gaaatgctaa aaaccgacgc ccgtcaggat ttgcgcctgt gggtgctgga
     5401 agatgaaagc cggatgatcg gttcgaatca cctgccggaa tgtctgcgcg agcgaatgac
     5461 tcaggcggcg attgcggtgg tagaagatcc gtttgagatc cgtcttgaac gcctgaatga
     5521 agagtatttc ttgcgtatgc atcatgattt tacccacgct tatggcgacg aacagggctg
     5581 gcaggaatat tgcgaatacc tgcatcacgg actttcggcg attaagcgtc ggctggggtt
     5641 acagcgctat aacgaactcg ctgcccagct ggatacggcg ctgacaacgc aactcaccac
     5701 cggcagcacc gacggtcatc tggcctggct ggtgccgtta cttaaagaat attacgaccc
     5761 aatgtatcgc tatcagcttg agaaaaaagc ggaaaaagtt gtctttcgcg gtgagtgggc
     5821 ggaagtggcg gtatgggtta aggcgcagtg atgggcggta ttctgaaacg gcgattgtcg
     5881 tgagaacaaa atggttgcac cagaatacag atataaaaaa accgacatga cagtcggttt
     5941 ttttattttt tccgcccaag acttggtggt tccgggcaga aattaaacag tcctcatcta
     6001 gcaagtgaat tataagtcag gatgcctaca tattcaaaca gctatgttgg cgatacaatc
     6061 ccgacgctag tagtgatact gcctcaaggg attaccgcag tgcggaaaag tacttttaag
     6121 tcctggtcag gatacaggtg gtgctgattc tggtgctcta atttggagag agacagtcct
     6181 catctagcac atgaagaaaa tcatagttat aggcaacgta agggtaagct gtgattgtct
     6241 ttctccctta agggagcaac ggatgaaaac ttttctcgat aaagttctca tcgttcttaa
     6301 ggcgctaatc gcgctgctgg aactgatccg tcagtttatt gaataactga ggaaacgtag
     6361 ctaaggccga aggtttacct ccttcggcct ttaaaaaaac cgacatgaca gttggtttca
     6421 tatttattgc caaaatgcac actgttcatg ccagatgcgg cgtaaacgcc ttatccggcc
     6481 tacacaatct tgcaaaatca atatattgca cgctcttcgt aggcctgata agcgtagcgc
     6541 atcaggcaat ctaacacctg tcatcacccc acaaactgcc tgaacagcgc cttaccctta
     6601 accagtcgcg cacccagcca gccgccgaaa agcgacagca gcagcgcacc gctgcacggc
     6661 agaacaatcc acaatcgcca gtctggctcc cacgggaaat caaacacttt cgcctgcaac
     6721 actgccagtg ccgtttccgc accaattgcg gccaccaggc cggaaacaaa cccaagcatg
     6781 gcgaacatca caccacaatg tcgtgcgcag cagttttttc cccgcaccga gtgtgcgcca
     6841 taccaccagc tcctgatgac gctggcgcat tcccacctgc acctgtgcca gcaacagcaa
     6901 cataccgcag gcggtgacca gcaccaccat cacctccagc gcccgactta cctgctccag
     6961 cacctgaccg acctgtttta aaatcgcgcc aatgtctaac aggctaatgg tcgggaactg
     7021 gcggttgagt tgcgtcaaca tgccgttgcc attctcccag cggaaactgg taagccagct
     7081 ctgcggttgc ccgtctaatg ccccttcagg gaaaataaaa tagaaattag gccgcagact
     7141 ttcccagtcc actttgcgca ggctggtcac tttagcgcgg aactcctggg tatcgcccat
     7201 aaaagtcacg gtatcaccga gggcaacgtt taagcgtttc gccagcccct cttccatcga
     7261 cacttcatcg gcttttggcg gccagttacc ggcgacaatc ggattatgat cgggccgcgt
     7321 attttgccag gtaagattga gttcgcgatt aagcgcctca tcttcattac cttctgtcgg
     7381 cttatcatta atcgccgtca gccgcgcccg cactaccgga taaaatgatt ccgggactat
     7441 atgatgttcc gcgaggaacg cttttagcgg tgctacctgt tctgtggcga tgttaattaa
     7501 aaagtagttc gggctttctg gcggtagctg ctgctgccag cggtcgagca gatcgccacg
     7561 cagcaccagc agcagtgcca gcagcataaa ggagagcgaa aatgccgaaa gctggcttaa
     7621 cgttgaccac ggctgacgta acaggcggct aaccgccagg cgcagaggca gcgatttcag
     7681 cgtcatgcgg cgaagtacat tcagcagcat ccagcccagc acaccgcaca gcaaagccag
     7741 tactaccgcg cccgccagca ccgcccaaag cagcatgctg ccacccatta atccggcgag
     7801 cagcagcaca accaccacac tgacaatcgg cagataaaac ttcagcggcc agacgttcgc
     7861 gaccacatca ttacgtaata cgcgtaaagg ctgcgttgcc agcaacaagc ggtatggtcg
     7921 tagccccacc agcagcgaga tgaccgtcat ggtgccaagc gcccacagcc acggccagag
     7981 gctggctggc ggtagtgcag caggcagaac gggcttgagc agcaccatca acacgttttc
     8041 gaacaacagg cctatggccc caccggtaac ggctgaaagc gtcagcacca tcaaccactg
     8101 accgacgatt agcttacgca gttgcgctcg ccctgccccc agcgttttga ggatcgccac
     8161 cagatcgtag cgactgcgac agtaatgatt catcgctacc gccaccgctg ccactgccag
     8221 cagcaaggtc agaagcgccg aaagcagcag gaactgttgc gagcgttcca tcgatcgccc
     8281 cagcgcgcct tcgtcctgtt ccagaccgta ccagcgttgt tcgggtttaa gctgaggtaa
     8341 caaccatttc tcatagccgt cgagctggtt ctcgttgccg ccgaatttat aacgccaggt
     8401 gacccgactc cccggctgca cggctccggt tttatcgaca tccgccagat tcatcatcag
     8461 acgcggagcc atctggaagg ggttaaaacc ggaatccggt tcctgaatca cttctccggc
     8521 aatccgcaag gtggcatcgc ccacgtcaat ggtgtcgccc gttttcaggt taagcagtgc
     8581 catcaggcgt ggggccagca atacgctgcc cgcctgcggt ttcaggccag ggggattagt
     8641 ttgcagaccg ccatacatcg ggtagatatc atccaccgct ttgacgttcg ccagctgcgg
     8701 tgtgtcgcct gcaaaggtca ttgtggcgaa agtcagctgc ttgccgactt tcaggccgcg
     8761 cttttgcgct tcctccagcc acgcttgcgg cacttcgcgt gaactgcgca acgcccgatc
     8821 gcccgccata aactcacggc tttgctggct taagcccttc tccatgcgat cgctgatatt
     8881 gcccagcgcc agcacgcagg ccaccgccag gcttagcgcc agccagacaa ttaatagcga
     8941 cggcgaacgc cattcgcgcc agaaccaacg tgcaatcatg cttcctcctg caactgcccg
     9001 ttcaccagcc gtaagcagcg gtcgcagcgt gccgccagtt gcaggtcgtg ggtcaccata
     9061 atcaacgtgg tgccatgttc acggttgagg gaaaacagca ggtcggcaat tttatcgccc
     9121 gtctggcggt caaggttgcc ggtgggttcg tcggcaaaca gcacatcagg tcgaccatta
     9181 aaggctcgtg ccagcgccac tcgttgctgt tcaccgccgg aaagctgtgc cggaagatga
     9241 tccagacgtt tacccagccc taactgttcg agcaacgctt tcgccccgtt acgactttcc
     9301 gcgctactct caccgcgcag cagagccgga agctcgacgt tttccagcgc gttaagggta
     9361 ggaattaaca taaatgactg aaaaacaaag ccgacgtgct tcgcgcgcaa ctttgcccgc
     9421 gcttcttcgt ccatattatg tagcggttgt cccaccagac tcacttcgcc actgctgccg
     9481 tcatcaagcc cggcgaggat cgccagcaag gttgacttac ccgatcccga ctcgcccacc
     9541 agtgcgatgg tctcgccacg tttgacaacc agctcaactc cggtgaggat ggagagttca
     9601 tgctccccct gaccgacgga cttcttaaga tgatgaactt caacaatgtt ttccgctggc
     9661 atttgccctt cctgttcctg gtcctgttaa ccttccgtgc cgccgcagcg gacacgttat
     9721 tgattctggg tgatagcctg agcgccgggt atcgaatgtc tgccagcgcg gcctggcctg
     9781 ccttgttgaa tgataagtgg cagagtaaaa cgtcggtagt taatgccagc atcagcggcg
     9841 acacctcgca acaagggctg gcgcgccttc cggctctgct caaacagcat cagccgcgtt
     9901 gggtgctggt tgaactgggc gcaatgatgg tttgcgtggt tttcagccac agcaaaccga
     9961 gcaaacgctg cgccagattt tgcaggatgt caaagccgcc aacgctgaac cattgttaat
    10021 gcaaatacgt ctgcctgcaa actatggtcg ccgttataat gaagccttta gcgccattta
    10081 ccccaaactc gccaaagagt ttgatgttcc gctgctgccc ttttttatgg aagaggtcta
    10141 cctcaagcca caatggatgc aggatgacgg tattcatccc aaccgcgacg cccagccgtt
    10201 tattgccgac tggatggcga agcagttgca gcctttagta aatcatgact cataaagcaa
    10261 cggagatcct gacaggtaaa gttatgcaaa aatcggtctt aattaccgga tgttccagtg
    10321 gaattggcct ggaaagcgcg ctcgaattaa aacgccaggg ttttcatgtg ctggcaggtt
    10381 gccgaaaacc gaatgatgtt gagcgtatga acaacatggg atttaccggc gtgttgatcg
    10441 atctggattc accagaaagt gttaatcgcg cagcagacga ggtgatcgcc ctgaccgata
    10501 attgtctgta tgggatcttt aacaatgccg gattcggcat gtatggcccc ctttccacca
    10561 tcagccgtgc gcagatggaa cagcagtttt ccgccaactt tttcggcgca caccagctca
    10621 ccatgcgcct gttacccgcg atgttaccgc acggtgaagg gcgtattgtg atgacatcat
    10681 cggtgatggg attaatctcc acgccgggtc gtggcgctta cgcggccagt aaatatgcgc
    10741 tggaggcgtg gtcagacgcg ctgcgcatgg agctacgcca cagcggaatt aaagtcagcc
    10801 tgatcgaacc cggtcccatt cgtactcgct tcaccgacaa cgtcaaccag acgcaaagtg
    10861 ataaaccagt cgaaaatccc ggcatcgccg cccgctttac gttgggaccg gaagcggtgg
    10921 tcgacaaagt gcgccatgct tttattagcg agaagccgaa gatgcgctat ccggtaacgc
    10981 tggtgacctg ggcagtaatg gtgctcgagc gcctgctgcc tgggcgcgtg atggacaaaa
    11041 tattgcaggg gtgagttgaa gcgcgcgctt aagcccccat gtcaaagaaa atatcgacaa
    11101 cagagagcga ctccatgtcc gtagaaaata ttgtcaacat taacgaatct aacctgcaac
    11161 aggttcttga acagtcgatg accactccgg tgctgttcta tttttggtct gaacgtagcc
    11221 agcactgttt gcagttaacc ccgatcctgg aaagcctcgc ggcgcagtac aacgggcaat
    11281 ttattctggc gaagctggac tgcgacgcgg agcagatgat tgccgcccag tttggtctgc
    11341 gcgcgattcc gaccgtgtat ctgttccaga acgggcaacc ggtagatggc ttccaggggc
    11401 cgcaaccgga agaggcgatc cgcgccctgc tggataaagt gctgccgcgc gaagaagagc
    11461 tgaaagcgca gcaggcgatg caactgatgc aggaaggcaa ttacaccgat gccctgccat
    11521 tgctgaaaga cgcctggcag ttgtcgaatc agaacgggga gatcggcctg ctgctggcag
    11581 aaacgctgat tgcgctgaac cgttctgaag atgcggaagc ggtgctgaaa accattccgt
    11641 tgcaggatca ggacacccgc taccaggggc tggtagcgca aatcgaactg ctgaagcagg
    11701 cggctgataa gccggaaatt caacagttgc aacagcaggt ggcggagaat ccagaagatg
    11761 ccgcactggc gacgcaactg gcgctgcaac tgcatcaggt tgggcgcaat gaagaggcgc
    11821 tggagttgct gttcgggcat ctgcgtaaag atctcaccgc cgcagacggt cagacgcgta
    11881 aaacgttcca ggagatcctc gctgcgctgg gtacgggtga tgcactggcg tcgaagtatc
    11941 gccgccagct gtatgcattg ttgtattgat attccgttgt tcggcacatg aaccacaacg
    12001 ccggatgcga tgctgcgcat ccggcatcgc atcatttctt cttcaattga gtcaccacca
    12061 actggtggcg cgaattataa aacttacgat aggttaaata gcaggcaata atggtcgaca
    12121 agctggcggt tgagagcagc ataaaggtca ccataatctg atatttaatc gccttcaccg
    12181 gatcaatccc ggcaaatatc agcccggaca tcattcctgg taaactcact aagccaaccg
    12241 tttttgctga atcgaccgtc ggaattaaag ccgcgcgaat actgtcgcga atcaatatcg
    12301 ctgaagcctg cttcggcgtc gcgccaagac tcagtttctc ctgaatctgt tgctgttcgc
    12361 taatgacccg ttgccctaaa ttgttgtaac acaaccccac cgctaccatg gcgttaccgg
    12421 caatcatccc ggcgacaggg atcacctgc
//
LOCUS       DAZXUK010000107        12380 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZXUK010000107 DAZXUK010000000
VERSION     DAZXUK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12380
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.107"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            328..1968
                     /gene="trg"
                     /locus_tag="ACH3L8_002326"
     CDS             328..1968
                     /gene="trg"
                     /locus_tag="ACH3L8_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFT4085410.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2006..2929)
                     /locus_tag="ACH3L8_002327"
     CDS             complement(2006..2929)
                     /locus_tag="ACH3L8_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFT4085411.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3146..4489
                     /locus_tag="ACH3L8_002328"
     CDS             3146..4489
                     /locus_tag="ACH3L8_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFT4085412.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4714..6369
                     /gene="opgD"
                     /locus_tag="ACH3L8_002329"
                     /gene_synonym="mdoD"
     CDS             4714..6369
                     /gene="opgD"
                     /locus_tag="ACH3L8_002329"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFT4085413.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6509..6733
                     /locus_tag="ACH3L8_002330"
     CDS             6509..6733
                     /locus_tag="ACH3L8_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFT4085414.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6796..7332
                     /gene="rimL"
                     /locus_tag="ACH3L8_002331"
     CDS             6796..7332
                     /gene="rimL"
                     /locus_tag="ACH3L8_002331"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFT4085415.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7327..8307)
                     /gene="ydcK"
                     /locus_tag="ACH3L8_002332"
     CDS             complement(7327..8307)
                     /gene="ydcK"
                     /locus_tag="ACH3L8_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFT4085416.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8431..9423
                     /gene="tehA"
                     /locus_tag="ACH3L8_002333"
     CDS             8431..9423
                     /gene="tehA"
                     /locus_tag="ACH3L8_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFT4085417.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9420..10013
                     /gene="tehB"
                     /locus_tag="ACH3L8_002334"
     CDS             9420..10013
                     /gene="tehB"
                     /locus_tag="ACH3L8_002334"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFT4085418.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10316..10972
                     /locus_tag="ACH3L8_002335"
     CDS             10316..10972
                     /locus_tag="ACH3L8_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFT4085419.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(11071..11481)
                     /gene="tnpA"
                     /locus_tag="ACH3L8_002336"
                     /pseudo
     CDS             complement(11071..11481)
                     /gene="tnpA"
                     /locus_tag="ACH3L8_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11504..12380
                     /locus_tag="ACH3L8_002337"
                     /pseudo
     CDS             11504..12380
                     /locus_tag="ACH3L8_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
BASE COUNT         3224 a         2902 c         3111 g         3143 t
ORIGIN      
        1 ttgatagaaa tatcggtcgg ggccttcgtc tttctgattc ccggttagcc tgaaaacaga
       61 aagtctcagg cacccgcagg catcctatga ggtttcctta gggacgaaaa taatcacttc
      121 acgaaattgc gtgctgtttt ccagaatttt tcgtcattcg ggttagccag tttagccatt
      181 cgttactctc ttcattccaa tagcattaat tttctctgca ataattgttg taaaaatgtg
      241 acgcaaagag gtttttggtc ataagtaatt accgtcaagt gccgatgact ttctatcagg
      301 agtaaacctg gacgagagac aacggtaatg aatacaactc cctcacagcg attaggtttt
      361 ttgcatcaca tcaggctggt tccgttattt gcctgcattc ttggcggtat cttagttcta
      421 ttcgcattaa gttctgctct ggctggctat ttcctctggc aggccgatcg cgatcagcgt
      481 gatgttactg cggagattga gattcggacc gggttagcga acagttcaga ttttttgcgt
      541 tcagcccgga tcaatatgat tcaggccggg gctgcgagtc gtattgcgga aatggaagca
      601 atgaagcgaa atattgcgca agccgaatcg gagattaaac agtcgcagca aggttatcgt
      661 gcttatcaga atcgaccggt gaaaacacct gctgatgaag ccttcgacac tgaattaaat
      721 caacgctttc aggcttatat cacgggtatg caacctatgt tgaaatatgc caaaaatggc
      781 atgtttgaag cgattatcaa tcatgaaagt gagcagatcc gaccgctgga taatgcttat
      841 accgatattt tgaacaaagc cgttaagata cgtagcacca gagccaacca actggcggaa
      901 ctggcccatc agcgcacccg cctgggtggg atgttcatga ttggcgcgtt tgtgcttgcc
      961 ctggtcatga cgctgataac atttatggtg ctacgtcgga tcgtcattcg tccactgcaa
     1021 catgccgcac aacggattga aaaaatcgcc agtggcgatc tgacgatgaa tgatgaaccg
     1081 gcgggtcgta atgaaatcgg tcgcttaagt cgtcatttac agcaaatgca gcattcactg
     1141 gggatgacag tagggactgt tcgacagggc gcggaagaga tttatcgtgg caccagcgaa
     1201 atttcagctg gcaatgcgga cctgtcatct cgcaccgaag aacaagcggc ggctatcgaa
     1261 caaactgccg ccagcatgga gcaactcact gcgacggtga aacagaatgc ggataacgcg
     1321 catcatgcca gcaaactggc gcaagaggct tctattaaag ccagcgatgg cgggcagacg
     1381 gtttccggtg tagtaaaaac gatgggcgct atctccacga gttcgaagaa aatttctgag
     1441 atcaccgccg tcatcaacag tattgctttc cagacgaata ttctggcact gaatgctgcc
     1501 gttgaagccg cgcgagcggg tgagcaaggg cgtggatttg ccgttgtcgc cagcgaagta
     1561 cggacactcg caagccgcag cgctcaggca gcgaaagaga ttgaaggctt gatcagtgaa
     1621 tcagtcaggt taattgacct ggggtcggat gaggtggcaa cggcagggaa aaccatgagc
     1681 actattgttg atgccgtcgc gagtgtcaca catatcatgc aggaaatcgc cgccgcctcg
     1741 gatgaacaaa gtagaggcat aactcaggtt agccaggcga tttctgaaat ggataaggtg
     1801 acgcaacaga atgcttctct ggtagaagag gcctcagcag cggcggtgtc ccttgaagaa
     1861 caggctgcac gattaactga ggcggtgaac gtattccgtc tgaacaaaca ttctgtgtcg
     1921 gcagaacctc gcggagcggg tgaaccagtt agtttcgcta cggtgtgaaa atgttcaagg
     1981 agggatcgac agatcccttc acctttcaga acggcattga ttttcgaata gcgttaatca
     2041 tcaactggca accagaagag aacgtcgcat ctacgcgggt cagtattcca atcggttcgc
     2101 ctgcaccatg tcccggaaca ggcagggcca ccagcgtggc atgacgcagg tcgtctttta
     2161 cagcgccaga agggacaaac cacacgtaat cgtattcaac ggtaagttga cgagatagcg
     2221 aagtagacag cgtttcgata caacccgaag gaattttaca gccctggctc tgcactaatg
     2281 catctgaatg ctggcgtggc gcagtgcctt ctggtgatac aacgaccggc cattccagca
     2341 cccggcttag cgttacgttc tcctgaagta gcgggtgatt agggcggaca accagcttca
     2401 acgattcaag aaacagcagt tcgtaattaa gcccggtcat cagttcagga tctgacatcc
     2461 gaccaatgcc gatatcgatt tccccggttt ttaaacccgc cagaatcata gggttactca
     2521 ttgtcgcaac ttgcaaggtc gtctcttttt gttgctgatg aaactgacct ataaccgaag
     2581 gtaatatccc cagtgccgca gtaggtagtg caccaaccct gacgacatca ttattaagac
     2641 cttctttacg atgaagcgac tgtccggcag tgttgatggc gtcaagaact ctgactgcat
     2701 gcgttaaaaa ttgttcgccg ggtaaggtaa gttgcgcccc ctgacgacca cgctcaaaca
     2761 agcgagcgcc cgtcagctgc tccagttcat tcaatgtatt agagagcgca ggttgactca
     2821 aattaagggt ttcagctgcg cgccccaaag ttccttgttg tgcgacagct acgaatgtat
     2881 gaaggtggcg caaacggatg cgctgactaa acagactatt tttttccata agcgatgtta
     2941 aaaacgaagc ggtgtcgctg acaagtgaag ttgtttgatt atgataactt gattgcaaaa
     3001 tattattaac aattaaagca attatgttac agcaaaatgg ataatattga tgttttcgcg
     3061 gcgagatcac agtttgtaaa ttcttcccgc cagagtgaat gcggttacct acactccaga
     3121 ttactgacca ctggaggcag acactatggc gaacagcatc acggcggatg agattcggga
     3181 acagttttcg caggcaatgt cagccatgta ccagcaagaa gttccgcaat atggcacgct
     3241 gctggaactg gtagctgatg tgaatctggc tgtgctggaa aacaatcctc aactgcacga
     3301 aaaaatggta aatgcagacg agctggcgcg actgaatgtt gaacgtcatg gggcgattcg
     3361 cgttgggact gcacaagagc ttgctactct tcggcggatg tttgccatta tggggatgta
     3421 cccggtgagc tattacgatc tctcgcaggc aggggtgccg gtacattcga cagcatttcg
     3481 gcccattgat gatgcttctc tggcgcgtaa tcccttccgc gtttttacat ccttactccg
     3541 ccttgagctt atcgagaacg aaattttgcg ccagaaagcg gcggagattc tgcgtcagcg
     3601 cgatatcttc accccacgtt gtcgacaact gttagaggaa tatgagcagc agggcggttt
     3661 taacgaaaca caggcacagg agtttgtgca ggaagccctg gaaacgtttc gctggcacca
     3721 gttagcaacg gtagatgaag aaacctatcg cgccttgcac aacgaacatc ggttgattgc
     3781 tgatgtggtc tgttttcctg gatgccatat caaccacctg acgccacgta cgctggatat
     3841 tgaccgggtg cagtcgatga tgcctgaatg cggaattgaa cccaaaattc tcatcgaggg
     3901 gccgccgcgc cgcgaggtac cgattttact acgccagacc agctttaaag cactggaaga
     3961 gacggtgttg tttgcggggc agaaacaggg cacgcatacc gcgcgctttg gtgaaattga
     4021 gcagcgtggc gtggcattaa cgccgaaagg gcgacaactg tatgatgatc ttctgcgtaa
     4081 cgctggaacc gggcaggata atctcactca ccaaatgcat ttacaggaaa ccttccgcac
     4141 ttttcctgac agtgagtttt taatgcgtca gcaagggttg gcatggttcc ggtaccgtct
     4201 gacgccttcg ggtgaggcgc atcgtcaggc gattcatcct ggagacgatc cacagccctt
     4261 aattgaacgt ggttgggtag tggcgcaacc catcacctat gaagatttct tgcccgttag
     4321 cgcggcgggg atcttccagt caaatctggg taatgaaacg cagacacgca gtcacggtaa
     4381 tgccagtcgc gaagcatttg agcaggcgtt gggttgtccg gttttggatg agttccagct
     4441 ttaccaggaa gcggaagaac gcagtaaacg tcgctgtggt ttgctttaaa ttctgaccat
     4501 ccgcctttgc aaaaatttgc ctgattttac aaacgaatca ggctcatccc atcgacataa
     4561 aaaaaatgcc gatttatgca tattctctca gttcaacaat tggattatta ataaatattg
     4621 tctagagtga gcggtcataa ataagcactt tcttgccgct gaaaacgacc agcgcgggac
     4681 cattcacaac accagaagga ctcactttca ggtatggatc gtagacgatt tattaaaggt
     4741 tcaatggcta tggccgccgt gtgcggtacc agcggcattg cttctctttt ttctcaggca
     4801 gcattcgcgg cagattctga tattgccgac gggcaaaccc agcgttttga cttctccatt
     4861 ctacagtcaa tggcgcacga cttagcgcaa acagcgtggc gtggtgcgcc tcgtccgtta
     4921 cctgacacgc tggcgacaat gacgccgcag gcttataaca gtattcaata cgacgccgaa
     4981 aaatcgctct ggcataacgt tgagaaccgt caactggacg ctcagttctt ccatatggga
     5041 atgggattcc gtcgccgcgt tcgtatgttt tctgtagatc cagcaacaca tctggcgcgt
     5101 gaaattcact ttcgcccgga gttgttcaaa tacaacgatg caggtgttga taccaaacaa
     5161 ttagaagggc aaagcgatct cggctttgcc ggttttcgcg tgtttaaagc ccccgaactg
     5221 gcgcgccgtg atgtagtatc atttctcggc gcgagttatt tccgcgccgt tgatgataca
     5281 tatcaatacg gtttgtcggc ccgcggcctg gcgatcgaca cttacaccga cagtaaagaa
     5341 gagttccccg actttaccgc cttctggttt gatacggtaa aaccgggggc aactaccttt
     5401 accgtttatg cgttgctcga tagcgccagc attactggtg cctataagtt cactatccat
     5461 tgtgagaaaa gtcaggtgat tatggatgtg gaaaatcacc tgtatgcgcg caaagacatt
     5521 aaacagctgg gcatttcgcc gatgaccagt atgttcagct gcggtactaa tgaacgtcgg
     5581 atgtgcgata caattcatcc gcaaattcat gactctgatc gtctgtccat gtggcggggc
     5641 aacggcgagt ggatttgccg tccgctgaat aatccgcaaa aattgcagtt caatgcttac
     5701 accgacaaca acccgaaagg gtttggttta ttgcaactgg atcgtgactt ctcccattat
     5761 caggacatta tgggctggta taacaaacgc ccaagtctgt gggtggaacc gcgtaacaag
     5821 tggggtaagg gcaccatcgg cctgatggaa atcccaacaa cgggcgaaac gctggataac
     5881 attgtctgct tctggcagcc agaaaaagct gtaaaagcag gtgatgagtt tgcattccag
     5941 tatcgtctgt actggagtgc gcaaccgcct gttcattgcc cattagcgcg cgttatggcg
     6001 acgcgtaccg gcatgggcgg tttcccggaa ggttgggcgc caggtgaaca ctatcccgaa
     6061 aaatgggcgc gtcgttttgc cgtcgatttc gttggtggtg atctgaaagc tgccgcgcca
     6121 aaaggcattg agccggtgat tacgctttcc agtggggaag cgaagcaaat cgaaattctc
     6181 tatattgaac ccatcgatgg ttatcgtatt cagtttgact ggtatccgac ttcggactcc
     6241 actgatccgg tcgatatgcg gatgtatcta cgttgtcagg gggacgctat cagtgaaaca
     6301 tggctgtatc agtatttccc gccagcgccg gataaacgtc agtatgttga cgaccgcgtg
     6361 atgagttaat cgttttttct tcggcacctt cttcgggagg tgccgtctgg ttaaacacga
     6421 tcccgctcgc atttttccct aagttaaatg agtaatctga tggtgtgtat ttcagataca
     6481 ccgtgtcagc cactaacagg gagtgcgtat gtttccagaa taccgagatt taatatcccg
     6541 tctgaaaaac gaaaatcctc gctttatgtc cttgttcgat aaacacaata aacttgatca
     6601 tgaaattgcc agaaaggaag gttccgacgg tcgagggtac aatgcggaag tggtccgcat
     6661 gaaaaaacaa aagctacagt taaaagatga gatgctcaaa atcctgcagc aggagagcgt
     6721 caaagaggtg taaactttcc taagccgcca gccaggcggc ttttttaaca acagcatgga
     6781 ttgactggag ataagatgac tgaaacgata aaagtaagcg aatcacttga attacatgct
     6841 gttgcggaaa gtcacgtcac acctctttat cagttaatct gtaaaaataa aacctggtta
     6901 cagcagtcgc taaactggcc gcagttcgtt cagagtgaag aggacacgcg aaaaacggtg
     6961 cagggtaatg tgatgttgca tcaacgcggc tatgccaaaa tgttcatgat tttcaaagaa
     7021 gatgaactta tcggcgttat ctcgtttaat cgtattgaac cactgaataa aactgctgaa
     7081 atcggctact ggctggacga atctcatcag gggcagggga tcatttctca ggcgctgcag
     7141 acattgattc atcattacgc tcagtctggt gaacttagac gcttcgtgat caaatgtcgg
     7201 gtggacaatc cgcaaagcaa ccaggtcgct ttgcgcaatg gttttatcct tgaaggttgc
     7261 ctgaaacagg ctgagttcct gaatgatgcc tatgatgatg tgaacttata cgcgcgtatt
     7321 atcgattcat aataaccctg ccagcggcgt tcgcgtaatg cgctcctcgc cgttaatgac
     7381 ttttggacca cggacatgaa ccgtgtcgcc atcaaaggct tcaacgacgg cgtggtcggt
     7441 taactccaca tggttttcaa ttatcaccgc acctgtaata cggctctcac cttgtattac
     7501 aacgtgctcg tcgagcagaa tcggtccacc acgtaccacg gcatttccgc caatcaagac
     7561 atgatgtttt aacacacaat taccttccac aatggcatat tccgccacct gcgaactgta
     7621 atgaatcgtc ggaatggcat cttcttctat gccagctttc acctgcgcgt gaccgtagac
     7681 tttagcgcaa tcgcatagcc agacgttgtt ctcttcatta ccttcaacac tggcaaaatc
     7741 aaaaacttcg gcgcgatgtt caataaaagc ataccggacg acggcatcgc cataaatttg
     7801 tgcctggtgg acaatacgcg aggcgctaac ccttgcgcga tcataaattt ggagcaggag
     7861 ttgatggtcg ggcgttaagc cttgtgcggc gacgatcata gaatgttgat caattaaggc
     7921 gtgaccaaat attctacatt gcacgtaaac taatgagtca tgaatggtga cactgtcact
     7981 tatatatgca ccctggctga tttcactatt atcgatccag acgttatccg ttgcgtaaac
     8041 ctctccccac agcacgctgg tgccggtgat acgcgtatta ccggaaatca ccgttccgcc
     8101 gaacgcaata gcattctggt cgtaaatcca gcaatttcct tcctgtgcta acaccgtttc
     8161 gcgatcgatc cagccgccag cggttcctgc tatcacatcg ttaaaatcgc taatggcgat
     8221 aatctgccgt aataacacac tttttttagt gccatcttcc tgataactaa aggctcgctg
     8281 ttcttcactt aggcgatatt tgcgcatagc gttttcccac aggtgactta ctataaccgt
     8341 agcaaattct gcggctctgg ctatgctcga gaaaattcca taaaatgcat ttcaaatata
     8401 ctttataaat taaacaaaat gagtaagaag atgcagagcg ataaagtgct caatttgccg
     8461 gcaggctact ttggtattgt gttggggacg atagggatgg gatttgcctg gcgctatgcc
     8521 agccaggttt ggcaggtcag ccactggtta ggggatgggc tggtgattct ggcgatgatc
     8581 atctggggat tattgactag cgcatttatt acccgactca tacgctttcc gcatagcgtg
     8641 ctggcggaag ttcgccatcc agtgctgagc agttttgtga gtttgtttcc ggcaacgacg
     8701 atgctggtgg cgattggttt tgttccgtgg tttcgcccac tggcggtgtg catgttcagt
     8761 tttggtgtcg tggttcagtt ggcttatgcc gcctggcaaa ctgcgggatt atggcgcgga
     8821 tctcaccctg aagaagctac cacgcctgga ctgtatctgc cgacagttgc caacaacttt
     8881 atcagcgcaa tggcctgtgg tgcgttgggc tacaccgacg ccggtctggt gtttttaggc
     8941 gcaggcgttt tctcatggct aagccttgaa ccggtgatct tgcagcgtct gcgcagttcg
     9001 ggagaattac ccacggcact gcggacatca ctcggcattc agctcgctcc tgcgctggtg
     9061 gcctgtagtg cctggctgag cgtcaacggc ggcgagggtg acacgctggc gaaaatgctt
     9121 ttcggttatg gactgctgca actgctgttt atgctacgtc tgatgccatg gtatctctcc
     9181 cagccattta atgcttcatt ctggagtttc tcgttcggcg tatctgcact ggcaaccacc
     9241 ggtttgcatc tggggagtgg cagcgataat ggatttttcc atacgctggc ggtgccgctg
     9301 tttatcttta ccaattttat tattgcaata ctgctcatcc gtacttttgc gcttctgatg
     9361 cagggaaaat tgttagtcag aaccgagcgc gccgttttaa tgaaagcaga ggacaaagaa
     9421 tgatcattcg tgacgaaaac tattttactg ataaatatga attaacccgc acacactctg
     9481 aagtactgga agcggtgaaa gtggttaaac cgggtaaaac gctggatctg ggctgtggca
     9541 atggtcgtaa cagtctttac ctggcagcca atggttatga tgttgacgca tgggataaaa
     9601 atgccatgag tatcgccaac gtcgagcgca ttaaatccat tgaaaatctg gataatttac
     9661 acacccgggt cgttgatctg aataacctca catttgatgg acagtacgat tttattcttt
     9721 cgactgtggt gctgatgttc cttgaggcta aaaccatccc agggttgatt gccaatatgc
     9781 aacgttgcac taaacctggt ggttacaacc tgattgtggc ggcgatggat accgctgatt
     9841 atccatgtac cgtcggcttc acgtttgcct tcaaagaggg agaattacgt cgatattacg
     9901 aaggctggga gagggtgaaa tacaatgaag acgtcggcga gctgcaccgc accgacgcca
     9961 acggtaatcg tattaaactg cgtttcgcca cgatgctggc acgtaaaaaa tgacccggta
    10021 agcacaaaac gcgtgaaaat tccccacgct gagatgattt actgttcttc ttttcggtaa
    10081 gcatattttt tatcgaaggg atgtgaaatt aatcacagta gtcgaagttt tttagcagct
    10141 taacttactg aaatttaact actgatgatt gacttagccc ctttttcggc attgactatg
    10201 tcgtctgaaa aggggctgaa aaatttattt tcaccaacac tttttttgcc acaacacgaa
    10261 gcggcgcttt ttgctataac ttagaaagta atataatcat ctcaggaaac tattcatgcg
    10321 taccacatca tttgcgaaag ttgcagcttt atgcggcttg ttggctctgt ctggttgtgc
    10381 ctctaaaatc acccagccag ataaatataa caattactct gatttaaaag aaacaacctc
    10441 agcaacaggt aaacctgttt tacgttgggt agacccgagt tttgatcaaa gcaaatatga
    10501 cagcatcgtc tggaacccaa tcacttatta tccggtacca aaaccttcta cccaggtagg
    10561 gcagaaagtt ctggataaaa ttttgaacta taccaacacc gaaatgaaag aagcgatagc
    10621 gcagcgtaaa ccagtggtta ccaccgctgg gccgcgtagt ctgattttcc gtggggccat
    10681 taccggtgta gataccagca aagaagggct gcaattctat gaagtggttc ctgttgcatt
    10741 agtggttgcg gggacgcaaa tggctacagg ccaccgtacc atggatactc gcctctattt
    10801 tgaaggtgag ctgattgatg cagcgactaa taaaccggtt atcaaagtcg ttcgtcaggg
    10861 cgaaggtaaa gacctgaata acgaaagtac gccaatggct ttcgaaaata ttaaacaagt
    10921 tattgatgac atggcgaccg atgccaccat gtttgacgtt aacaaaaagt agttccagac
    10981 gcgccatcgt tagatggcgc tttttatccg gtgcgccgta aaaccccatc cttcagggcg
    11041 gggatataag gcgcggtttt ccacctgacc aggtgtttgc tgttgttcaa tgtattggcg
    11101 gatgacggat attggcgcac ctccgcagct actggcaaaa tagccaggac tccacaaaac
    11161 gcctttgtag taatacctga ctgcaatatc tggtcgatct cgtcgcagta acctacccga
    11221 tacgcctttg aggctgttta ccagactgga tatcgccagt ttggggggat agttaattaa
    11281 cagatggacg tgatctggtt cgccatccat ttcaaccagt tcagcttcaa aataagcaca
    11341 tacatttgaa aagtaagtgc gtagtttttc tgtcgcgtca tggtcaaaaa tctggcgtcg
    11401 gtatctggtg acaaagacta ggtgaacatg catcaggaaa acacaatgcc ttccacgccg
    11461 gatatcggtt tcttttttca cagaccaaag tatcatttga cctgtgaaac gattacaggc
    11521 atttaaattc cagttaagac caggtggtca acaggagtgt gaaatgaggc gcttcgcagg
    11581 cgcatgtcgt ttcgttttca atcgtgctct ggcacgtcag aatgagaatc atgaggccgg
    11641 taataaatac atcccttacg ggaaaatggc ttcctggctg gttgagtgga aaaatgccac
    11701 tgaaacgcaa tggcttaaag attctccctc acagccattg caacagtcac tgaaagacct
    11761 tgagcgggct tacaaaaact tcttccggaa gcgggctgct tttccccgat tcaaaaagcg
    11821 gtgacagaat gatgcattcc gctacccgca gggcgttaag ctcgatcagg aaaacagccg
    11881 tatttttctg ccgaaactgg gctggatgcg ctaccggaat agccgtcagg tcacgggtgt
    11941 tgtgaaaaat gtcactgtca gccagtcctg cggtacgtgg tacatcagta ttcagacaga
    12001 aagtgaagta tccactccgg ctcacccttc agcatcaatg gtcgggctgg atgctggcgt
    12061 ggctaaactc gccacgctgt cagatggcac agtctttgag cctgtaaaca gttttcagaa
    12121 aaaccagaag acgctggcga gacttcagcg acagttaagc cgcaaggtca aattcagcaa
    12181 caactggcag aagcagaaac gcaaaataca gcgactgcat tcctgtatcg caaatatccg
    12241 cagggactac cttcacaaag tcacaacggc cgtcagcaaa aaccacgcaa tgatagtcat
    12301 tgaggatttg aaggtcagca acatgtcaaa gtcagcagcg ggtacggtca gccagccggg
    12361 gcgcaatgtc cgggcaaaat
//
LOCUS       DAZXUK010000108        12287 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZXUK010000108 DAZXUK010000000
VERSION     DAZXUK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12287
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.108"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            119..1585
                     /locus_tag="ACH3L8_002338"
     CDS             119..1585
                     /locus_tag="ACH3L8_002338"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFT4085420.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            1703..2689
                     /gene="yeiR"
                     /locus_tag="ACH3L8_002339"
     CDS             1703..2689
                     /gene="yeiR"
                     /locus_tag="ACH3L8_002339"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFT4085421.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            2728..3441
                     /gene="lpxT"
                     /locus_tag="ACH3L8_002340"
     CDS             2728..3441
                     /gene="lpxT"
                     /locus_tag="ACH3L8_002340"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFT4085422.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            3853..4419
                     /gene="mepS"
                     /locus_tag="ACH3L8_002341"
     CDS             3853..4419
                     /gene="mepS"
                     /locus_tag="ACH3L8_002341"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFT4085423.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            4600..6156
                     /locus_tag="ACH3L8_002342"
     CDS             4600..6156
                     /locus_tag="ACH3L8_002342"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFT4085424.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            6238..8052
                     /locus_tag="ACH3L8_002343"
     CDS             6238..8052
                     /locus_tag="ACH3L8_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFT4085425.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            8053..9147
                     /locus_tag="ACH3L8_002344"
     CDS             8053..9147
                     /locus_tag="ACH3L8_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFT4085426.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            9147..10172
                     /locus_tag="ACH3L8_002345"
     CDS             9147..10172
                     /locus_tag="ACH3L8_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4085427.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            10174..11763
                     /gene="yejF"
                     /locus_tag="ACH3L8_002346"
     CDS             10174..11763
                     /gene="yejF"
                     /locus_tag="ACH3L8_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFT4085428.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(11767..12111)
                     /locus_tag="ACH3L8_002347"
     CDS             complement(11767..12111)
                     /locus_tag="ACH3L8_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFT4085429.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
BASE COUNT         2938 a         3048 c         3067 g         3234 t
ORIGIN      
        1 cactcgttga ttgatcgccc tcacacttca tcgcattaac aatccagacc aatttcaatt
       61 gctgtcatat aactttacac tgtcattgtt aatttatcgt tactaagacg tgactcctat
      121 gaagacaatt gcctccgtta cgctcccgca tcatgtacac gctccacgct acgatcgcca
      181 gcagttgcaa tcacgtatcg ttcattttgg ctttggcgcc tttcaccgcg ctcatcaggc
      241 gttactgacc gatcgtgtgc tgaatgccca gggcggcgac tgggggatct gtgaaatcag
      301 cttgttcagc ggtgatcaac tgatgagcca gctccgcgca cagaaccatt tatataccgt
      361 gctggagaaa ggtgcggacg gcaatcaggt gataattgtc ggtgccgttc acgaatgcct
      421 taatgcaaaa ctggattcct tagcggcaat tattgagaaa ttttgcgagc cacaggtggc
      481 aattgtttcc ctgacgatta ccgaaaaagg ctattgtatt gacccggcca ccggtgcact
      541 cgacaccagt aatccgcgga ttattcacga tctacaaacc cctgaagaac ctcactccgc
      601 accgggtatt ctcgtcgaag cactgaaacg ccgccgtgag cgcagcctta caccgtttac
      661 cgtgctctcc tgcgacaata ttcccgacaa tggccatgtg gtgaaaaacg cggtgctgag
      721 aatggcagaa aaacgttcgc cagaactcgc cgggtggata aaagaacacg tcagttttcc
      781 gggaaccatg gtcgaccgca ttgttccggc tgcaaccgac gaatcactgg cagaaatcag
      841 ccagcatctg ggggtaaatg atccctgcgc gattagctgc gaaccgttta tccagtgggt
      901 ggttgaagat aacttcatcg ctgggcgtcc tgcctgggaa gtcgcaggtg tacaaatggt
      961 gaatgatgtc ctgccatggg aagagatgaa actgcgtatg cttaatggca gccactcttt
     1021 tctcgcttat ctgggttacc tctcaggatt cgcctatatc agtgattgca tgcaggatcg
     1081 cgtatttcgc tatgctgcca gaacattaat gctggatgag caagcgccga cactgcaaat
     1141 taaagatgtc gatttaacac aatatgcgga taagttaatt gcacgttttg ctaatccgtc
     1201 gctgaaacat aagacctggc aaatcgcgat ggatggcagc cagaaattac cgcaacgtat
     1261 gctggcaggt attcgcatac atctggggcg tgaaacggac tggtcgttgc tggcattagg
     1321 cgttgctggc tggatgcgtt acgtcagcgg cgttgatgat gccggaaatg ccattgatgt
     1381 tcgcgatccg cttagcgata aaattcgcga acttgttgcg ggcagcagca gtgaacaacg
     1441 cgtaaccgcc ctgctttccc tgcgtgaagt tttcggtgat gatctgccag ataacccgca
     1501 ttttgtgcag gccatcgaac aagcctggca acaaatcgta caattcggcg cacatcaggc
     1561 gctattaaac accctcaaaa tttaacgatt tctgcggtta aagcggatga agctcacctt
     1621 cgtccgctct ccccttctct tttctgcctt ttttagccag gattaacgct cagttaactt
     1681 accagagtat tgcggagccg ttgtgaccag gaccaacctc atcaccggtt ttctcggcag
     1741 cgggaaaacc acgtcgattc ttcatctgtt agcccataaa gatcccaacg aaaaatgggc
     1801 ggtactggtt aatgaatttg gggaagtcgg aattgatggt gctttgctcg ccgatagcgg
     1861 cgcattgctg aaagagatcc ccggcggctg catgtgctgc gttaatggtt tacccatgca
     1921 ggtagggttg aataccttac tgcgtcaggg aaaaccagac cgcttgttga tagagccgac
     1981 cgggctgggc catccgaaac agatcctcga tctgttaacc gcaccagtct atgaaccgtg
     2041 gatagatctg cgcgccacct tgtgcattct cgatccacgc ctgctgctgg acgaaaaaag
     2101 cgccagcaat gaaaacttcc gtgaccagct ggctgccgca gacatcattg tcgccaataa
     2161 atccgaccgt gcgacgcccg aaagtgagca agcgctacag cgttggtggc agcaaaatgg
     2221 tggcgatcga caattaattc acagtgagca tgggaaagtt gacggtcatc ttctggattt
     2281 gccgcgtcgc aatttagccg agttgcccgc cagcgccgcg cattctcatc agcatgtcgt
     2341 gaaaaaaggg ttagcagcgt taagcctgcc agagcatcaa cgctggcgtc gcagtctgaa
     2401 cagcgggcaa ggatatcagg cctgcggctg gatattcgac gctgatacgg tattcgacac
     2461 cattggcatt ctggaatggg cgcgacttgc accggtggaa cgcgtcaaag gcgtgctgcg
     2521 tattcccgaa gggctggtac gaatcaaccg tcagggcgat gacctgcaca ttgaaacgca
     2581 aaacgttgcg ccaccggaca gccgtattga gctgatttcc agcagcgaag ctgactggaa
     2641 tgccttgcag agcgcgctgt tgaagcttcg tttagcgact accgcgtaag gttgcctgcg
     2701 tttttcagta agataattag agaaaatatg attaaaaatt tgccgcaaat agtgttgttg
     2761 aatattgtcg gccccgcgct gtttctttcc tggtatattc ccgttaatca tggattctgg
     2821 ttgccgattg atgcggatat tttttatttc tttaatcaga aactggtcga aagtaaggcc
     2881 tttttatggc tggttgcatt gactaacaat cgcgccttcg acggttgttc actgctggcg
     2941 atgggtatgt tgatgctgag tttctggctg aaagaaaacg cccctggcag acgacgtatc
     3001 gtgattatgg gtctggtcat gctattaact gcagtggtat taaaccagct gggtcaggca
     3061 ttaattcctg taaaacgggc cagcccaaca ttgactttta ccgatattaa ccgcgtcagc
     3121 gaactgctct ctgttcccac gaaagatgcc tcacgagata gctttcccgg cgatcacggc
     3181 atgatgctgc ttattttttc ggcattcatg tggcgttatt tcggcaaagt tgcaggcctt
     3241 atcgccctta ttatttttat ggtttttgca tttcccagag taatgattgg cgcacactgg
     3301 tttactgaca tcattgtcgg ttcgatgacc gtgatattga tcggtttgcc ctgggtgttg
     3361 ctgacgccat taagtgatcg attaatcacc ttttttgaca aatcactacc aggaaaaaac
     3421 aaacatttcc aaaacaaata actcacagta attaacatca tcagggttat ttttatagtg
     3481 aggataatcc tgatgatgcg caccgtgctt tcatctatcg aacgcaaaaa tcattctcta
     3541 agtaaatgaa tggattgcat gcgtttcact caattgtact ttaattgacc aaccccgctt
     3601 attaactttc tgtatcactt tttcttataa aaaatcatgt aaaaccgctc gccaagaccg
     3661 caccaatcgg gtaatctcga actcgttttg cctcggcggt agattatcct cacagcatat
     3721 aattttgtgc gttagtccac agatttggcc ttaaggaatt gtttcaacat gcccaggtaa
     3781 ttagtctcgt gtcgcttggc atttttttat aacgatattt gtcgttaagg acttcaaggg
     3841 aaaacaaaca acatggtcaa atctcaaccg attttgagat atatcttgcg cgggattccc
     3901 gcgattgcag tagcggttct gctttctgca tgtagtgcaa ataacaccgc aaagaatatg
     3961 catcctgaga cacgtgcagt gggtagtgaa acatcatcac tgcaagcttc tcaggatgaa
     4021 tttgaaaacc tggttcgtaa tgtcgacgta aaatcgcgaa ttatggatca gtatgctgac
     4081 tggaaaggcg tacgttatcg tctgggcggc agcactaaaa aaggtatcga ttgttctggt
     4141 ttcgtacagc gtacattccg tgagcaattt ggcttagaac ttccgcgttc gacttacgaa
     4201 cagcaggaaa tgggtaaatc tgtttcccgc agtaatttgc gtacgggtga tttagttctg
     4261 ttccgtgccg gttcaacggg acgccatgtc ggtatttata tcggcaacaa tcagtttgtc
     4321 catgcttcca ccagcagtgg tgttattatt tccagcatga atgaaccgta ctggaagaag
     4381 cgttacaacg aagcacgccg ggttctcagc cgcagctaat aaaccgtttg gatgcaatcc
     4441 cttggctatc ctgacgagtt aactgaaagc actgcttagg cagtgctttt ttgttttcat
     4501 tcatcagaga aaatgatgtt tccgcgtctt gatccaggct atagtccggt cattgttatc
     4561 ttttaaatgt tgtcgtaatt tcaggaaatt aacggaatca tgttcatacg cgctcccaat
     4621 tctggacgta agctcctgct tacctgcatt gtagcaggcg tgatgattgc gatactggtt
     4681 agctgccttc agtttttagt ggcctggcat aagcacgaag tcaaatacga cacactgatt
     4741 atcgacgtac aaaagtatct cgatacctat tttgccgacc tgaaatccac tactgaccgg
     4801 cttcagccgc tgaccttaga tacctgccag caggctaacc ccgaactgac cgctcgcgcg
     4861 gcgtttagca tgaatgtccg tacgtttgtg ctggtgaaag ataaaaaaac attctgttca
     4921 tctgcgactg gtgagatgga cattccactc aatgaattga ttccggcgct cgacattaat
     4981 aaaaacgtcg atatggcgat cttacccggc acgccgatgg tgccgaacaa acccgcaatc
     5041 gtcatctggt atcgcaaccc tttgctgaaa aatagcggtg tgtttgccgc tctgaatctc
     5101 aacctgacgc cttctctctt ttatagttca cggcaggaag attacgatgg cgtcgccctc
     5161 attattggca atactgcgct atctaccttt tcttcacgtt tgatgaacgt taatgaatta
     5221 accgacatgc cggtccgtga aactaaaatt gcgggcattc ctctgaccgt tcggctttat
     5281 gcggatgact ggacatggaa cgatgtgtgg tacgcatttt tactgggcgg catgagtgga
     5341 actgtcgttg gcctgctttg ctattacctg atgagcgtac gtatgcgccc cgacagagaa
     5401 atcatgaccg ccatcaagcg cgaacaattt tacgtggcgt atcaaccggt ggtggataca
     5461 caagctttgc gggtaacggg cctggaagta ctgctacgct ggcggcatcc tgtagcggga
     5521 gaaattcccc cggatgcctt cattaacttt gccgaatcgc aaaagatgat tgtgccgctg
     5581 actcagcacc tgtttgagtt aattgcccgc gatgccgcag aattagaaaa agtactgccg
     5641 gtaggcgtca aatttggcat taacattgcg ccggaccatc tgcacagcga aagctttaaa
     5701 gcagatatcc agaaactgct cacttccctg cccgcacacc atttccagat tgtgctggaa
     5761 attaccgagc gcgatatgct gaaagagcaa gaagccacac aactcttcgc ctggctgcac
     5821 tcggtcggcg tagaaattgc tattgatgac ttcggcaccg ggcacagcgc gcttatctat
     5881 cttgagcgtt ttacgctcga ttatctgaaa attgaccgtg gatttatcaa cgccatcggt
     5941 acggaaacga tcacttcacc cgtacttgac gcggtgctga cgctggcgaa acgtctcaat
     6001 atgctgacag ttactgaagg ggtcgaaacg ccggaacaag cgcgatggct aagcgaacgc
     6061 ggcgttaatt tcatgcaagg ctactggatt agtcacccgt taccgctgga cgattttgtt
     6121 cgctggctga agaaaccgta tacgccgcag tggtaaggtg tgcttacgtc ccttattatt
     6181 catagtgaaa gcatgccgga ttgcggctaa tgatgagtaa aaggaaatcc gttgcagatg
     6241 attgtgcgca tactgctgct gtttatcgct ctgttcacct tcggtgcgca ggcgcaggct
     6301 atcaaggaaa gctatacctt tgccgtgctg ggcgaacccc ggtacgcgtt taatttcaac
     6361 cattttgatt atgtgaaccc cgccgcgcca aaaggtgggc agataacgtt gtcagccctc
     6421 ggtaccttcg ataatttcaa ccgctatgca ctgcgcggca acccgggcgc acgcaccgag
     6481 cagctgtacg acacgttatt tacgacttcc gatgacgaac caggcagtta ttacccgctg
     6541 attgctgaaa gtgcacgcta tgctgacgat tattcctggg tggaggtcgc tattaatccg
     6601 cgcgcccgtt ttcatgatgg ttctcccatt actgcccgcg atgtagagtt tacttttcaa
     6661 aaatttctga ccgaaggcgt gccgcaattt cgtctggtct acaaaggcac caccgtcaaa
     6721 gccattgcgc cgttaaccgt gcgcattgag ttagctaaac ccggcaaaga agatatgttg
     6781 agtctgtttt cgctgccggt atttccagaa aagtactgga aggatcacaa acttagcgac
     6841 ccgctcgcca cgcctccgct tgccagtggt ccgtaccacg ttacgtcctg gaaaatgggg
     6901 caaaatattg tctattcccg cgtgaaagat tactgggcag caaacttacc ggtaaaccgt
     6961 ggacgctgga atttcgacac cattcgctac gattattacc tcgatgataa tgtcgccttt
     7021 gaagcgttta aagcaggtgc ctttgatttg cgtatggaaa acgacgctaa aaactgggcc
     7081 acgcgttata ccggtaaaaa tttcgataaa aaatacatca tcaaagatga gcaaaagaac
     7141 gaatcagccc aggatacgcg ctggctggcg tttaatatcc aacgtgcggt attcagcgat
     7201 cgccgggtgc gggaagcaat cactctcgcc tttgactttg aatggatgaa caaggcgttg
     7261 ttttacaacg cctggagtcg cacgaacagt tattttcaaa ataccgaata cgcggccaga
     7321 aattaccccg acgccgcgga gctggtgctt ctggcaccaa tgaaaaaaga tctaccgcca
     7381 gaagtcttca cacaaatcta ccagccgccg gtatctaaag gcgatggcta cgatcgtgac
     7441 aacctgttaa aagccgacaa acttctcaac gaagcgggct gggtgctgaa gggtcagcaa
     7501 cgcgttaatg ccacaacggg tcagccgctc agctttgaat tattgcttcc cgcaagcagc
     7561 aatagtcagt gggtattgcc gttccagcac agcctgcaac ggctgggtat caatatggac
     7621 attcgcaagg tggataactc gcaaatcact aaccgcatgc gcaatcgcga ctatgacatg
     7681 atgccgcgac tatggcgggc gatgccgtgg cccagttccg atttacagat ttcctggtca
     7741 tcggaatata tcaattccac ttataatgcc cccggcgtgc aaagcccggt tatcgactca
     7801 ctgatcaacc aaattattgc cgcgcaggga aataaagaaa aattactgcc gttggggcga
     7861 gcactggatc gcgtattaac gtggaattat tacatgctgc caatgtggta catggcggaa
     7921 gaccgtctcg cctggtggga taaattctcc caacccgctg tacgccctgt ttacagcctg
     7981 ggtatcgata cctggtggta tgacgttaat aaagcggcca aactgccgtc agccagacaa
     8041 cagggagagt agatgggcgc ttacctgatt cgccgtctgt tgctggtgat cccaacgtta
     8101 tgggctatta tcactatcaa ctttttcatc gtgcaaattg cgcctggcgg tccggtcgat
     8161 caggccatcg ccgccattga gtttggtaat gccggagtat tacccggcgc aggcggtgaa
     8221 ggtgttcgtg ccagccatgc gcaaacgggt gtcggcaata tcagcgacag taattaccgt
     8281 ggcggacgcg gattagatcc agaagtgatc gctgagatca ctcatcgcta cggtttcgat
     8341 aagccgatcc acgaacgtta cttcaaaatg ctctgggact acatccgctt tgattttggt
     8401 gatagcctgt ttcgcagcgc ctcggtgctg acgctgatta aagacagtct gccggtttcc
     8461 atcaccctcg gattgtggag cacgctgatt atctatctgg tgtcgattcc gttaggcatt
     8521 cgcaaagctg tttataatgg gagtcgcttt gacgtctgga gtagcgcatt tatcatcatc
     8581 ggctacgcca ttccggcctt tttgtttgcc atcctgctga ttgtcttctt cgcgggcggc
     8641 agctatttcg acctgttccc tctacgcggc ctggtttccg ctaactttga ttcgctgccg
     8701 tggtatcaga aaatcaccga ttatctgtgg catatcacgc taccggtgct ggcgacagtg
     8761 attggtggct ttgcggcgct gaccatgctg accaaaaact cattccttga tgaagtgcgc
     8821 aagcaatacg tggtgaccgc gcgtgcgaaa ggggtaagtg agaaaaacat tctctggaaa
     8881 catatgttcc gcaacgccat gctgctggtg attgccggtt ttccggcgac gtttatcagc
     8941 atgtttttta ccggctcgct gctgattgag gtgatgtttt cactcaatgg tcttggctta
     9001 ctgggctacg aagcgaccgt ctcgcgcgat tatcctgtaa tgtttggtac cttgtatatt
     9061 ttcaccctga ttggcctgct gctgaatatt gtcagtgata tcagctatac gctggttgat
     9121 ccgcgtatag attttgaggg acgttaatgt cgcgactcag ccccgtcaat caggcccgtt
     9181 gggcgcgttt tcgtcataac cgtcgcggct actggtcgtt atggattttc ctcgttttgt
     9241 ttggtttgag tttgtgttct gaacttatcg ctaacgataa accgttgctg gtgcgttatg
     9301 acggcagttg gtatttcccg ttgttgaaaa actacagcga aagcgatttt ggcggcccgc
     9361 tggcaagtca ggctgattat caggacccgt ggctgaaaca acggctggaa aataacggct
     9421 gggtactgtg ggcaccgatt cgctttggtg ctaccagtat caactttgct accgataagc
     9481 ccttcccttc tccaccctcc cggcaaaact ggctgggaac ggatgcaaac ggcggcgatg
     9541 tgctggcacg cattctctat ggcacgcgga tctcggttct gtttggcctg atgctgactc
     9601 tctgttccag cgtgatgggc gtgctggcgg gggcgctaca aggctattac ggcggtaaag
     9661 tcgatctctg ggggcaacgc tttattgaag tatggtcggg gatgccgacg ctgtttttga
     9721 ttattttact ttccagcgtc gtacagccta acttctggtg gctgctggca attactgtct
     9781 tgtttggctg gatgagtctg gtcggcgtgg tgcgggcgga gtttttacgt actcgtaatt
     9841 tcgactacat tcgtgcggca caggcgcttg gcgtcagcga tcgcagtatc atcctgcgtc
     9901 atatgttgcc taatgccatg gtcgcgaccc tcaccttttt accgtttatt ttatgtagtt
     9961 cgataaccac cctgacctcg ctcgatttcc tcggcttcgg tctaccgctc ggttcaccgt
    10021 cactcggtga actgctgtta caagggaaaa ataaccttca ggccccgtgg cttgggatca
    10081 ccgccttctt gtcggtggcg atattattgt ctttgctgat ctttattggt gaagccgtcc
    10141 gcgacgcatt tgatcctaat aaggcggtgt agcatgacgc aaactctgtt agcgattgaa
    10201 aatttgtcgg tgggttttcg ccatcagcaa accgtacgta cagtagtcaa tgatgtttca
    10261 ctacagattg aggctggcga aacgctggcg ctggtgggtg agtcaggttc aggcaaaagc
    10321 gttaccgcgc tgtcaatttt acgcctgctc ccttccccgc cggttgaata tctctccggc
    10381 gatattcgtt ttcatggcga atcgctgctt catgccagcg atcaaacgtt gcgcggtgta
    10441 cgcggtaata agatcgccat gatttttcag gaaccgatgg tgtcgttaaa tccattgcat
    10501 accctggaaa aacagcttta tgaagtgctt tcactccacc gcgggatgcg tcgggaagcg
    10561 gctcgtggcg aaattcttaa ctgccttgat cgcgtcggta tccgccaggc ggcaaaacgg
    10621 ctgacagatt atccgcatca gctctccggc ggcgaacgcc agcgggtgat gattgcgatg
    10681 gcgctgttaa cgcgaccgga attattaatt gccgatgaac cgaccaccgc actggacgtc
    10741 tctgtccagg cgcagatttt acagctgttg cgcgaactgc aaggcgagct gaatatgggc
    10801 atgctgttta ttactcataa cctcagcatt gtcagaaaac tggcccaccg cgtggcggta
    10861 atgcaaaacg gtcgctgtgt cgagcaaaat aacgccgcta cgctatttgc ctcacccact
    10921 catccttaca cacaaaagct actcaacagt gaaccgtcag gcgatccggt accgttgcca
    10981 gaacctgcct caacgttgct ggatgttgaa cagcttcagg ttgccttccc cattcgcaaa
    11041 gggattttga agcgcattgt ggatcataat gtggtggtga aaaacatcag ttttacgcta
    11101 cgggcgggtg aaacactggg tttagtgggc gagtctggtt ccgggaaaag cacgacggga
    11161 ctggcgctgc tgcgactgat tacctctcag ggcagcatcg tctttgacgg tcagccactg
    11221 caagatttaa atcgccgcca gctgttacct actcgtcatc gtattcaggt ggtatttcag
    11281 gatccaaact cctcgctcaa cccacgactc aacgttttgc agattattga ggaaggctta
    11341 cgggttcacc agccgacgct ttctgccgca caacgcgaac aacaagtgat agccgtgatg
    11401 catgaagtgg gattagatcc tgaaacacgc caccgttatc cggcggagtt ctctggtggt
    11461 cagcgacaac gtattgcgat tgccagggca ttaattctta agccctcgct gatcatactt
    11521 gatgaaccga catcatcact cgacaaaacg gtacaggcgc aaatattgac gcttttgaaa
    11581 tcattgcaac aaaagcatca actggcctat ttgtttatca gccacgattt gcacgttgtc
    11641 cgcgcgttat gtcatcaggt tatcgtactg cgacaagggg aagtagtgga acaaggaccg
    11701 tgcgcgcgcg tgtttgccac accgcagcag gagtatacgc gtcagttact ggcgttgagc
    11761 tgacgcttaa aaaggattgt agtttgaaaa gggttcggca attgccacac caaaattttt
    11821 caatcggcat gttgcagcaa actcgtcctg gcgatttaca aacaggcacg gctcaccttc
    11881 acactccaac accgaacatg gaacttcgat gtcgcttaac gcctggctta gtttatacat
    11941 caccgaccat gcattatcac catccgggct aagcaattta agcgccacca ggctgttgtc
    12001 accgcacggt ccattttgcg gaatcggttc aaccttcgaa cctgcgaaac cagcagacca
    12061 gcgatagttc tgcggcagtc gatggacgat tgagagttgt aatgaagtca cttattttcc
    12121 ccggaagtac attaacttca caatttgttt acatcaattt taacacatca tcaacaatcc
    12181 gtctttatac agatcgtaaa gattgatgcc tcgcctgcgt ggcctctatg gctctgattt
    12241 aagtaatatc tgcgggcgtg gtcacgtttg cggggctata tgtaccc
//
LOCUS       DAZXUK010000109        12262 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZXUK010000109 DAZXUK010000000
VERSION     DAZXUK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12262
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.109"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..98
                     /locus_tag="ACH3L8_002348"
     CDS             <1..98
                     /locus_tag="ACH3L8_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFT4085430.1"
                     /translation="FEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            95..496
                     /locus_tag="ACH3L8_002349"
     CDS             95..496
                     /locus_tag="ACH3L8_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFT4085431.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            507..1247
                     /locus_tag="ACH3L8_002350"
     CDS             507..1247
                     /locus_tag="ACH3L8_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFT4085432.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1306..1692
                     /locus_tag="ACH3L8_002351"
     CDS             1306..1692
                     /locus_tag="ACH3L8_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFT4085433.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1701..2030
                     /locus_tag="ACH3L8_002352"
     CDS             1701..2030
                     /locus_tag="ACH3L8_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFT4085434.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2002..5043
                     /locus_tag="ACH3L8_002353"
     CDS             2002..5043
                     /locus_tag="ACH3L8_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFT4085435.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5043..5372
                     /locus_tag="ACH3L8_002354"
     CDS             5043..5372
                     /locus_tag="ACH3L8_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFT4085436.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5372..6070
                     /locus_tag="ACH3L8_002355"
     CDS             5372..6070
                     /locus_tag="ACH3L8_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFT4085437.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            6075..6818
                     /locus_tag="ACH3L8_002356"
     CDS             6075..6818
                     /locus_tag="ACH3L8_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFT4085438.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6782..7357
                     /locus_tag="ACH3L8_002357"
     CDS             6782..7357
                     /locus_tag="ACH3L8_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFT4085439.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSDGWYQVRIAG
                     RDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIM
                     ASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7418..10897
                     /locus_tag="ACH3L8_002358"
     CDS             7418..10897
                     /locus_tag="ACH3L8_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFT4085440.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10965..11564
                     /locus_tag="ACH3L8_002359"
     CDS             10965..11564
                     /locus_tag="ACH3L8_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFT4085441.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHRSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11628..>12262
                     /locus_tag="ACH3L8_002360"
     CDS             11628..>12262
                     /locus_tag="ACH3L8_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFT4085442.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT         2886 a         2913 c         3926 g         2537 t
ORIGIN      
        1 catttgaaga tgcccgcgac cgcatcattg ctgcggaaat gccgaaacag ctggggtatg
       61 ccctgaaaca acaactgagg ttacatctga gtaaatgaac cgacacacac aaatccgtca
      121 gagcgtgctg gcacgccttc gggaacagtg tggagacagc gccacgtttt ttgacgggct
      181 tccggcattt attgatgcgc aggaactgcc cgccgtggcg gtgtggctga gtgatgcgca
      241 gtacaccgga aaaatgacgg atgaggacga ctggcaggct gttctgcata ttgccgtttt
      301 cgtccgtgca caggcgccgg attctgagct ggatacgtgg atggagaaca ccatatttcc
      361 ggccatgaag gatattccgg tgctttccgg actcatcgac accatgatcc cactcggttt
      421 taactatcaa cgtgataatg agatggccac ctgggcgatg gcggaaatca cgtaccagat
      481 cacgtacacg aattaaggag gtggtaatga ccacaccaaa tccactggca aagacgaaag
      541 gtgcggggac gacgttctgg atgtataccg gcaacggcga tgcgtttgcg aaccctttgt
      601 cggacactga ctggctgcgt cttgcgatgg tgaaggatct gcaacctggc gaaatgaccg
      661 ctgatgcaga agatgacact tatctcgatg atgaagatgc agactggaaa acgacaaccc
      721 aggggcagaa atccgttggt gatacttcgg cgacgctggc ctggcgtccg ggtgacagcg
      781 ggcagaaaaa actggttcag ttgttcgact ccggtgaagt ctgcgcgttt cgtatcaaat
      841 atcccaacgg cactgttgat gttttccgcg gctggctgag ctcactgggt aaaaccattg
      901 cctcaaaaga cgtgatgacc cgcactgtga aaatcagcgg tgtggggcgt ccgtatctgg
      961 cagaggaagg cactgaaacc gtgggtgtta ccggactgac ggtggcaccg gcatctgtca
     1021 gtgtcaaagc gggagcaacc accacactga cctttacagt aaaacctgac ggggccagtg
     1081 acaaagcgat cagtgtgcat tcgtcagatc ctcagactgc ttcggtgacc ctgagcgggc
     1141 ttgtggccac ggtgaaaggc gtgaagcagg gcagtgtcag catcgtgggc atgacctctg
     1201 acggagagtt tgtggcagtg gctgcggtga ccgtcagcgc accataacag gacgatactc
     1261 atcattgccc cggttatccg gggctttttg catccggaga acatgatgtt tctgaaacag
     1321 gatacgttta attatgaaaa acagtccgtg gtgctcagtg agctgtccgg gctgcagaga
     1381 attgaatatc tgacgtttgt tcagcagcga acggcaaagt ttgatgcaca ggagggagaa
     1441 ctgccggagg ctgaacgaca gattgctttt ctgcgtatgg gaatggatat caatgcctgg
     1501 ctggtttccc gctcactgtg gaatgctgag cagtctcagg atgttgagac gctttgcgca
     1561 tccattatga caacatggtc gtatgatgcg ctgggcgcgg gggcggagag ggttctgtcg
     1621 ctgagcggta tggggaccat tgagaatgcc ggggatgatg atcatgaggc gctgacgccg
     1681 gaaaagtcct gacgcgggaa atgcagtttg tcatgcggct tgcccgggag ttccggcggg
     1741 cagactggcg gcggatgctg tcggatatgt cggccactga gcttggtgag tggggcgatt
     1801 atttccggat gcagagcttc agtgatgtgt ggatggatgc gcagtttgcc tcgctgaagg
     1861 cattgatcgt gagaatggtg tccggcagca gcgatgctgc ggtggctgat ttcagccttt
     1921 taccggaaga gaacgggata ccggagcgaa cggacgaaga actgatgcat cttggggaag
     1981 gtatttccgg aggtgtgcgt tatggaccag atagccaacc tggtcattga tttggggatt
     2041 gatgcggcag agtttaaaaa tgaaattccc cgtatcaaaa accttctgaa tggtgcagcc
     2101 agcgatgcag aacggtcttc tgcccgtatg cagcgtttta tggagcgtca gactcaggcg
     2161 gcccggcaga caatgcaggc ggcttcttcg gctgcaacag cagcatcagc ccatgcgcag
     2221 acggtggaga agaatgcacg ggctcatgaa cgcatggccc gtgaggtgga acaaacccgt
     2281 ctgcgcgtgg atgccctgaa tcagaaaatg cgcgaggaac aggcgcaggc cagggcactg
     2341 gcggaggcgc aggataaagc ggctgccgcc ttttatcgcc agattgacag tgtgaaacag
     2401 gccggtgcgg ggcttcagga attacagcgt attcagcagc agatccgaca ggccagaaac
     2461 agtggcgggg ttggtcagca ggattatctg gcgctgattt cggagatcac ggcgaaaacc
     2521 cgtgccctga cgcaggcaga ggaacaggcc acccggcaga aagcggcgtt tatccgccag
     2581 cttaaagagc aggcaacccg ccagaatctg tcgtcttctg agttgcttcg cgccagggcg
     2641 gctcagctgg gggtaagcag tgctgcagaa gtgtatatcc gcaaaatgga gcgggcagga
     2701 aaagccacac attcgctggg gctgaaaagt gcggcagccc ggcgggagct gggggtgtta
     2761 atcagtcaga tggcgcgagg caattttggt gcgctgaggg gatccgggat aacgctggct
     2821 aaccgtgccg gatggacagg cgcactgatg tcgccgaaag gcatgatgac tggcggcgtt
     2881 attggcggac ttgtcgcggc ggtcctgggt ctgggtaaag cctggcatga cggccggaag
     2941 gagggcgaga aatttaaccg tcagctggcg ctgacgggac attatgccgg tgtcactgtc
     3001 gggcagttgt ggaaactcag ccgggccata tccgggaatg gtatcacgca acatgcggca
     3061 gccggtgcgc tggcacaggt ggtgggcagt ggggcatttc atggaaacga tattggtatg
     3121 gtggcgaaag ctgccgcaca gatggagcga tcggttggcc agtcggtcag cgataccata
     3181 aatcagttta agcggctgaa ggatgatcct gtaaatgccg cgaaggcgct ggacaatgcg
     3241 ctgcattttt tgaatgccac tcagcttgag cagatacgcg tccttgggga gcaggggcgg
     3301 tccagtgatg cggcccggat cgccatgtct gcgctggcag aggaaaccgg taaacgcaca
     3361 tctgatattg ataataatct caatgcgctg ggtagtacgc tgcaaacctt gtctgactgg
     3421 tggaagcagt tctgggatgc ggccatgaat attggtcgtg aagactcgct ggatgcgcag
     3481 attgatgcgt tacaggaaaa aattcagcgc gcgaaaaaat atccgtggac aaacgcctcc
     3541 acacaggtgg agtacgatca gcagcgtctt aacgatcttc aggagaaaaa acgccggaag
     3601 gatttgcagg atgcaaaagc gcaggcagaa cggaattacc aggagcaaca gaaacgccgg
     3661 aatgctgaaa atgccgcgct gaaccggatg aatgaaacgg aagctgcacg acatcagcgg
     3721 gaaattgcgc gtattaatgc catgcagtac gccgaccagg ctgtcaggga tgcggcgata
     3781 cagcgtgaaa atgaacgtta cgaaaaagcc attaagaaaa atacacgggc aacccgtaat
     3841 gatgaggcca cccggttatt gctgcagtac agtcagcagc aggcacaggt ggaagggcag
     3901 attgctgctg ccagacagtc agcaggcatt gccactgaaa ggatgactga agcgcataaa
     3961 cagcttctgg ccctgcagca gcgcatcagc gacctggacg ggaaaaagct gacggcagat
     4021 gaaaagagtg tgctggcccg taagaatgag ctgattcagg cgctgacgct gctggatgtg
     4081 aaacagcagg agctgcagaa acagacagcg cttaacgacc tgagaaaaaa aacggttcag
     4141 ctgaccagcc agctggcaga caaagaacgt gcactgcgtg agcagcacaa tctggatatt
     4201 gccactgcag gtatggggga taagcagcgg cagcgctacc aggcacagtt gcgcatccgg
     4261 caggaatacc ggcaacagtt gcaacagctt gagaatgaca gtcgccagaa aggcacttac
     4321 gggacggagg actaccggag ggctgaggag gtgctgaagg ggagcctgaa gcgacaactg
     4381 aatgaaaaca aacgctactg gcaggaactg gaagtggcgc agggcgactg gaaaaacggt
     4441 gccatgcggg cgtttcagaa ttttacggcg gatgcggata atgcggcggg aacggcagaa
     4501 cagatgttta cagtggcatt cagcagtgcc ggtaatgcac tggcgacatt ctgtaccacc
     4561 ggtaagctga attttaaatc cttcacctct tccctgttgt cagatatggc cagaattatg
     4621 gcacagatgg ccatgatgca ggcggtaaag ggcgtcggtt ctttattcgg cttcacgact
     4681 aatgctgatg gcggtgttta ccagtctgct gatttgagtc gctacagtgg cacggtggtt
     4741 aaccgtccga cgttttttgc ttttgcaaaa ggcgcgggtg tgatggggga agcgggacct
     4801 gaagccattc tgccactgcg tcgcggtgct gatggtaagc tgggggttgt ggcggatact
     4861 ggtggttcag gcatggtgat gtttgccccg cagtacaaca ttgagatcaa taacgacggc
     4921 acgaacgggc agataggtcc ggctgccctg aaggtggttt atgacctcgg gaaaaaagcg
     4981 gccgcggact ttatgcaaca gcaggcccgt gatggtggcc ggttaagtgg agcataccgg
     5041 taatggagac gtttcactgg aaagtgcgcc cggatatgaa tgtggtatca gagccgaaag
     5101 tggtgacagt gaagctgggc gatggttatg aacagcgtcg tgcggcggga ctgaataacc
     5161 agttgtcgac ttacagcgtg acgatacgtg ttcgtaaagg tgaacaccca tctttaaaag
     5221 cctttctgga acggcacggt ggcgtccgcg catttcagtg gacgccacct tatgactgga
     5281 aactgatcag ggtggtttgt cgtaaatggt cggcaagcgt gggggcgtta tgggtgactg
     5341 taacggccga ttttgaacag gtggtgaact gatgcaggat atccggcagg aaacactgaa
     5401 tgaatgcacc cgtgcggagc agtcagcccg ggtggagctc tgggaaatcg atctgacaga
     5461 ggtcggtggt gagcgttatt ttttctgtaa tgagcagaac gaaaaaggtg agccggtcac
     5521 ctggcagggg cggcagtatc aggcataccc cattcagggg tcggggtttg aactgaacgg
     5581 caggggctgt gctgcccgtc cgacactgac ggtctctaac ctgcacggca tggtcaccgg
     5641 gatggcggaa gatctgcaga gtctggtcgg cggaacggtg gtccggcgta aggtttacgc
     5701 ccgttttctg gatgcggtga acttcgtcaa cggaaacagc gacgccgatc cggaacagga
     5761 ggtgatcagc cgctggcgca tcgagcagtg cagcgaactg agcgcggtca gtgcctcttt
     5821 tgtgctggcc acaccaacgg agacggatgg cgcggttttc ccggggcgta tcatgctggc
     5881 gaatacctgt atgtggactt accgttctga tgagtgtggt tacacgggca gggcagtggc
     5941 tgacgagttc gacaaaccaa caacggatat ccggaaggac aaatgcagca agtgtatgcg
     6001 cgggtgtgag ttgcgcaaca ataccggtaa tttcggcggt ttcctttcca tcaataaact
     6061 ttctcagtaa atccatgaca cagacagaat cagcgattct ggcgcacgcc cggcgatgtg
     6121 cgccagcgga gtcgtgtggc ttcgtggtga gaacgccgga gggagacagg tatcttccca
     6181 gcgagaatat ctccggtgag ccggaggaac ggttccggat ggctccggag gactggctgc
     6241 gggcacaaat gcagggtgag attgtggcac tggtccacag tcatcccggt ggtctgccct
     6301 ggctgagtga ggctgaccgg cggctgcagg tgcagagtga tttgccgtgg tggctggtct
     6361 gccgggggac gattcacaag ttccgctgtg tgccgcatct caccgggcgg cgctttgagc
     6421 acggggtgac ggactgttac acgctgttcc gggatgctta tcatctggcg gggattgaga
     6481 tgccggattt tcatcgcggg gatgactggt ggcgtcacgg tcagaatctc tatctggata
     6541 atctggaggc cacagggctg tatcaggtgc cgttgtcatc agcacaaccg ggcgatgtgc
     6601 tgctgtgctg ttttggttca tcggtgccga atcatgccgc catttactgc ggcgatggtg
     6661 agctgctgca ccatattcct gaacaactga gtaaacgaga gaggtatacc gacaaatggc
     6721 agcgacgcac acactccctc tggcgtcacc gggaatggca cgcatctgcc tttacgggga
     6781 tttgcaacga tttggccgcc gcatcgacct tcgtgtgaaa acgggggctg aagccatccg
     6841 cgcactggcc acacagctcc cggcgtttcg tcagaaactg agtgacggct ggtatcaggt
     6901 gcgcattgcc gggcgtgatg caggtgaaac cgaattgtct gcccgtctta atgagccgct
     6961 ggcaaatggt gcagtgatcc acatagtacc gcgtctggtg ggagctaaaa gtggcggtgt
     7021 gtttcaggcg gtgctggggg cagctgttat ggcggttgct atatggatgc cgggggtagg
     7081 aattatggcg agtaatctgc tgttttctct cggtgccagt atgacgcttg gcggtgttgc
     7141 acagatgctg gcaccgaaag ccagaactcc ccgtacacag acaacggata acggcaaaca
     7201 gaacacctgt ttctcctcac tggataacat ggttgcccag ggcaatgttc tgcctgttct
     7261 gtacggtgaa atgcgcgtgg ggtcgcgggt ggtatctcag gagatcagca cggcagacga
     7321 aggggatggt ggtcaggttg tggtgattgg tcgctgatgc aaaatgtttc atgtgaaacc
     7381 gcctgcgggc ggttttgtcg tttatggagc gtgaggaatg ggtaaaggca gcagtaaggg
     7441 gcataccccg cgcgaagcga aggacaacct gaaatccacg cagttgctga gtgtgattga
     7501 tgccatcagc gaagggccgg ttgacggtcc ggtggatgga ttaaaaagcg tgctgctgaa
     7561 cggtacgccg gtcctggata gcgaggggaa gacaaacttt tccggtgtta cggtggtgtt
     7621 ccgcgccggc gagcaggagc agacaccgcc ggaggggttt gaatcttccg gctcagagac
     7681 tgtgctgggt acggaagtga aatacgacac gccgatcacc cggaccatca cgtcggcaaa
     7741 cattgatcgt ctgcgcctga ccttcggtgt gcaggcactg gtggaaacca cctcaaaggg
     7801 ggaccggaat ccgtcggaag tccgcctgct ggttcagata cagcgtaacg gtggctgggt
     7861 gacggaaaaa gacatcacca ttaagggcaa aaccacttcg cagtatctgg cctcggtggt
     7921 ggtgggtaac ctgccgccgc gcccgtttaa tatccggatg cgcaggatga caccggacag
     7981 caccacagac cagctgcaga acaaaacgct ctggtcgtcg tacaccgaaa tcatcgatgt
     8041 gaaacagtgc tacccgaaca cggcgctggt cggcgtgcag gtggattcag agcagttcgg
     8101 taaccagcag gtgagtcgca attatcatct tcgcgggcgc attctgcagg tgccgtcgaa
     8161 ctataacccg cagacgcggc aatacagcgg tatctgggac ggaacgctta agccagcata
     8221 cagcaacaac atggcctggt gtctgtggga catgctcact catccgcgct acggcatggg
     8281 gaaacgtctt ggtgcggcag atgtggacaa atgggcgctg tatgtcatcg gccagtactg
     8341 cgaccagtca gtgccggacg gatttggcgg cacggagccg cgcatcacct gtaacgctta
     8401 cctgaccaca cagcgtaagg cgtgggatgt tctcagtgat ttctgctcgg cgatgcgctg
     8461 tatgccggta tggaacgggc agacgctgac gttcgtgcag gaccgaccgt cggataaagt
     8521 gtggacctat aaccgcagta atgtggtgat gccggatgat ggcgcgccgt tccgctacag
     8581 cttcagcgcc ctgaaggacc gccataatgt cgttgaggtg aactggattg atccggacaa
     8641 cggtcatgag acggcgacag agcttgtgga ggacacgcag gccattgtcc gttacggtcg
     8701 taacgtcacg aagatggatg cctttggctg taccagccgg ggacaggcgc accgcgccgg
     8761 gctgtggctg attaaaacgg aactgctgga aacgcagacc gtggacttca gcgtcggcgc
     8821 agaagggctt cgccatgtac cgggtgatgt cattgaaatc tgcgatgatg attatgccgg
     8881 tatcagcacc ggtggtcgcg tactggcggt gaacagccag acccggacgc tgacgctcga
     8941 ccgtgaaatc acgctgccgt cctccggtac cacgctgata agcctggttg acggaaatgg
     9001 caatccggtc agcgtggagg ttcagtccgt caccgacggc gtgaaggtga aagtgagccg
     9061 ggttcctgac ggcgttgcag aatacagcgt gtgggggctg aagctgccga cgctgcgcca
     9121 gcgcctgttc cgctgcgtga gtatccgtga gaacgacgac ggcacgtatg ccatcaccgc
     9181 cgtgcagcat gtgccggaaa aagaggccat cgtggataac ggggcacact ttgacggtga
     9241 ccggcgcggc acggtgaatg gtgtcacgcc gccagcggtg cagcatctga ccgccgaagt
     9301 caccgcagac agcggggaat atcaggtgct ggcgcgatgg gacacgccga aggtggtgaa
     9361 gggtgtgagc ttcctgcttc gcctgaccgt ggcagcggac gacggcagtg agcggctggt
     9421 cagcacagcc aggacgacgg aaaccacata ccgcttcagg cagctggcgc tggggaatta
     9481 cagtctgaca gtccgggcgg taaatgcccg ggggcagcag ggcgatccgg cgtcggtatc
     9541 gttccggatt gcggcaccgg cagcgcctgt cactattgaa ctgataccgg ggtattttca
     9601 gataacggtg gtcccgaaac tggctgtata tgacccgacg gtgcagtttg agttctggtt
     9661 ttcggaaaag cggattgcgg atatcaggca ggttgaaacc agcgcccgct atcttggcac
     9721 ggcgctgtac tggatagccg ccagtatcaa tatcaggccg ggccatgatt attattttta
     9781 cgttcgcagt gtgaacaccg ttggcaaatc ggcatttgtg gaggccgtcg gtcgggcgag
     9841 cgatgatgcg gaaggttatc tgagttttta taaagggttg atcaataaaa cgcatctcgg
     9901 caaggagttg tggacgcaga ttgataacgg tcagcttgcg ccggacctga ctgaaatcag
     9961 gacgtccatt acgaatgtca gcaatgaaat cacgcaaacc gtcaataaaa aactggaaaa
    10021 tcagagtgct gcaatccagc agatacagaa agttcaggtt gatacaaata ataacctgaa
    10081 cagcatgtgg gctgtgaagc tgcaacagat gaaggacgga cgcctttata ttgcgggtat
    10141 cggtgccggt attgagaata cgccagcagg aatgcagagt caggtgctgc tggcggcaga
    10201 caggattgcg atgattaatc ctgcgaatgg caacacaaag ccgatgtttg ttggtcaggg
    10261 cgatcagata ttcatgaacg acgtgttcct gaaacgcctg acggctccga ccattaccag
    10321 cggcggtaat cctccggcat tttccctgac acctggcgga cggctgacgg cgaaaaatgc
    10381 cgatatcagc ggtaacgtga acgcgaactc cgggacgctc aacaacgtca cgattaataa
    10441 gaactgtcgg gttctgggaa aattgtccgc gaaccagatt gaaggcgatc tcgttaaaac
    10501 agtgggcaaa cctttcccac gggactcccg ggcaccggag aggtggccat cagggaccat
    10561 taccgtcagg gtttatgacg atcagccgtt tgatcggcaa attgttattc ccgcggtggc
    10621 gtttcgcggt gctaaacatg agcggaagaa taacaatatt tattcgtcat gccgcctgat
    10681 agtgaagaaa aacggtgctg aaatttataa ccgaacgacc ctggataata cgctgatata
    10741 tacgggtgtt attgatatgc ctgccggtca cggtcacatg acgctggagt tttctgtatc
    10801 ggcatggctg gtaaatggct ggtatcccac agcaagtatc agcgatttgc tggttgttgt
    10861 gatgaagaaa tccacagcag gtatcagtat cagctgaatt ttataaccca gatacgggca
    10921 ccagaaatgg tgcctttttt attgcagaaa agcgagaggt aattatgcgt aaactttatg
    10981 ctgccatttt gtccgcagcc atctgtctgg ctgtatccgg tgcgcctgca tgggcgtctg
    11041 aacatcggtc cacgctgagc gcggggtatc ttcatgcctc gacgaacgct cccggcagtg
    11101 atgatctgaa cgggattaac gtgaaatacc gttatgagtt tacggacacg ctggggctgg
    11161 tgacgtcatt cagctatgcc aacgctgaag atgagcaaaa aacgcattac agtgataccc
    11221 gctggcatga ggattccgtg cgtaaccgct ggttcagcgt gatggcgggg ctgtctgtgc
    11281 gcgtgaatga atggttcagc gcgtatgcga tggcgggcgt ggcttacagc cgtgtgtcga
    11341 ccttctccgg ggattatctc cgcgtaactg acaacaaggg gaaaacgcac gatgtgctga
    11401 ccgggagtga tgacaaccgc cacagcaaca cgtctctggc gtggggggct ggcgtgcagt
    11461 ttaacccgac cgaatccgtg gccattgacc ttgcttatga aggttccggc agtggcgact
    11521 ggcgcactga cggtttcatc gtgggtgtcg gttataagtt ctgattagcc aggtaacaca
    11581 gtgttatgac agcccgctgg ttcaggcggg ctttttgtgg ggtgaatatg gcagtaaaga
    11641 tttcaggtgt actgaaagat ggggcgggta aacctgtcgt aaattgtgcg attgaactgc
    11701 gggccagaag aaccagtccg accgttgtgg cacacgttgt tgccacttgc gtgacggaca
    11761 ataacggtgc ttatgtgatt gaggctgagc cggggtatta cgaggttgcg cttcactgta
    11821 acggctggca gccaacccgt gtcggggata ttgatgtggc accgactgat gcaccgggga
    11881 cactgaacgc gtttctgaat gcaccaaagg atggtgattt acgtccggag gtgatgaagc
    11941 gctttgagga aatggtggcg caggcgcagc agagtgccgg ggctgcagcc ggaaacgcac
    12001 agcagacggc gcaggatgtg gcggcagccg caacggcccg tgatgatgca caacgttttg
    12061 cggagaaagc ccgacaggat gcaaccgtca cagctgagga cagaaaggcc actgcggaag
    12121 atgtgacaag cacaggagca aatgcagccg cagccggaca gagcgcacag gatgccgcag
    12181 gttatgcccg cgcagcagaa caggccaaaa atgacattga tgctgcgctg accggcactc
    12241 tgaaaatggc taaccatctg tc
//
LOCUS       DAZXUK010000110        12220 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZXUK010000110 DAZXUK010000000
VERSION     DAZXUK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12220
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.110"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..493)
                     /locus_tag="ACH3L8_002361"
     CDS             complement(<1..493)
                     /locus_tag="ACH3L8_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFT4085443.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="ACH3L8_002362"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="ACH3L8_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFT4085444.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="ACH3L8_002363"
     CDS             complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="ACH3L8_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFT4085445.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2459..3325
                     /gene="folD"
                     /locus_tag="ACH3L8_002364"
     CDS             2459..3325
                     /gene="folD"
                     /locus_tag="ACH3L8_002364"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFT4085446.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3327..3539
                     /gene="ybcJ"
                     /locus_tag="ACH3L8_002365"
     CDS             3327..3539
                     /gene="ybcJ"
                     /locus_tag="ACH3L8_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFT4085447.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3647..4156
                     /locus_tag="ACH3L8_002366"
     CDS             3647..4156
                     /locus_tag="ACH3L8_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFT4085448.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="ACH3L8_002367"
     CDS             complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="ACH3L8_002367"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFT4085449.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5751..6245
                     /gene="ppiB"
                     /locus_tag="ACH3L8_002368"
     CDS             5751..6245
                     /gene="ppiB"
                     /locus_tag="ACH3L8_002368"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFT4085450.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6248..6970
                     /gene="lpxH"
                     /locus_tag="ACH3L8_002369"
     CDS             6248..6970
                     /gene="lpxH"
                     /locus_tag="ACH3L8_002369"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFT4085451.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7088..7597
                     /gene="purE"
                     /locus_tag="ACH3L8_002370"
     CDS             7088..7597
                     /gene="purE"
                     /locus_tag="ACH3L8_002370"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFT4085452.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7594..8661
                     /gene="purK"
                     /locus_tag="ACH3L8_002371"
     CDS             7594..8661
                     /gene="purK"
                     /locus_tag="ACH3L8_002371"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFT4085453.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8798..9691)
                     /locus_tag="ACH3L8_002372"
     CDS             complement(8798..9691)
                     /locus_tag="ACH3L8_002372"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFT4085454.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9688..10335)
                     /locus_tag="ACH3L8_002373"
                     /pseudo
     CDS             complement(9688..10335)
                     /locus_tag="ACH3L8_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10338..11570
                     /gene="allC"
                     /locus_tag="ACH3L8_002374"
     CDS             10338..11570
                     /gene="allC"
                     /locus_tag="ACH3L8_002374"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFT4085455.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11581..>12220
                     /gene="allE"
                     /locus_tag="ACH3L8_002375"
     CDS             11581..>12220
                     /gene="allE"
                     /locus_tag="ACH3L8_002375"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFT4085456.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2966 a         3158 c         3118 g         2978 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1321 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1381 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1441 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1501 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1561 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1621 cgacacccgt tgctgttgtt gtatttgtac tggatacaat ggccaataaa ttatcattga
     1681 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1741 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1801 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1861 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1921 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1981 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     2041 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2101 actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa aatatattca
     2161 ccacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2221 gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca gatttacaac
     2281 gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg gtgcgcctaa
     2341 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
     2401 ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
     2461 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
     2521 aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
     2581 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt
     2641 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
     2701 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
     2761 accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga
     2821 cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
     2881 caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
     2941 cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgttgct
     3001 cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc atcacgtaga
     3061 aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat
     3121 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     3181 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg
     3241 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     3301 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     3361 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     3421 tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca
     3481 aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt gttgcctgat
     3541 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     3601 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     3661 tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag gtctgttatt
     3721 tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta aatttggcgt
     3781 tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt ttgcgtttgt
     3841 tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     3901 ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg taaccactgg
     3961 cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     4021 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     4081 gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     4141 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca attacttacg
     4201 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt taagacggtc
     4261 acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     4321 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     4381 ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt gagatgccat
     4441 tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg ccatatcaaa
     4501 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     4561 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     4621 gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt tcagctggct
     4681 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     4741 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     4801 cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     4861 gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     4921 gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag gacgacccgc
     4981 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     5041 catccggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca gctggtcgag
     5101 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgct
     5161 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     5221 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca aagcatcaaa
     5281 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     5341 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt
     5401 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     5461 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     5521 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     5581 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     5641 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     5701 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     5761 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     5821 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     5881 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     5941 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     6001 caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     6061 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     6121 ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     6181 acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     6241 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     6301 caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg cgctgtatat
     6361 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     6421 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     6481 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga cattattgcc
     6541 ggaagaaaag gtgctcgaac tttatggtcg ccgggtgttg attatgcatg gcgacacgct
     6601 gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac cctggctgca
     6661 aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgcgc
     6721 gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     6781 tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc atacccatcg
     6841 cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg tactgggtgc
     6901 ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc tgattcattt
     6961 tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt tttccttgct
     7021 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     7081 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     7141 cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg tcccgcacca
     7201 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     7261 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     7321 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     7381 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     7441 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc
     7501 gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc agaccgacga
     7561 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     7621 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     7681 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     7741 atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg
     7801 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct tttcgataag
     7861 ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg
     7921 tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc
     7981 ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg ttacggtgaa
     8041 tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg cgcgcgcggc
     8101 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     8161 cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc cgaagagatg
     8221 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     8281 accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag cggtcactgg
     8341 acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat taccgatctg
     8401 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     8461 gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga caaagaagtc
     8521 cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgact
     8581 gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgccagcgg cgtgatttgg
     8641 gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg cgtaaacgcc
     8701 ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc aagcgtcgca
     8761 tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata caggtccccg
     8821 cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg ggtttactac
     8881 ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
     8941 caaatggcgc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
     9001 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
     9061 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccatctt
     9121 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
     9181 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc acccggcgca
     9241 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
     9301 ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga tcatcaggcg
     9361 aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt gcgctcaagc
     9421 tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca tccagcggat
     9481 acggttctac ctctttccac gccagattct gtaacgccag cagccccacc tgcggcccgt
     9541 tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg ggtacagcac
     9601 tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg agtaaggcgt
     9661 tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt gttggccaag
     9721 ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca gcgaatcaat
     9781 cgcaacagcg gtacgtttcg ggcaactcag agcatggaca aagtgcagga gtggcgaggc
     9841 gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg taacgagttg
     9901 aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc gcatctaaag cgccataata
     9961 ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta atccggggcc
    10021 tttacccagc cagtgccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    10081 acgaaactgg cgtaattcag cgggcagcgg atcgcttgcc gccatcgcca gtggcccgaa
    10141 aagtccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag gatgcgaagg
    10201 cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac gcccgaggcg
    10261 aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    10321 gaattgcgtg cggcgaaatt acacattccc gtcaagctat agaagaaacg ctgccctggc
    10381 tttcctcttt tggcgctgac ccaacgggtg ggatgacccg tttactttat tcgccggaat
    10441 ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc aagtgggctg gaaacacgtt
    10501 tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg
    10561 ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg
    10621 gcgcgctggc ggcgtggctg gcaattgact ggttgaaaac gcaatacggc gcaccgttac
    10681 gtacggtcga agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgtcttct
    10741 ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg
    10801 ccaaaggaaa tagttttgcc gatgcgatga aggcttgcgg atttactctg ccgaacgccc
    10861 cactaactcc gcgtcaggct ataaaagcct ttgttgaact gcatattgaa cagggctgtg
    10921 tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg cagcgtcgct
    10981 atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc
    11041 gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga
    11101 tgggcgatcc gctggttctg acctttggca aagtagagcc gcgcccgaat acggtaaatg
    11161 tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgct gccgtgctgc
    11221 gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg
    11281 gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg
    11341 ccaccctgac agaattgtgt gaaagagaaa aactgaatta ccgggtgatg cacagtggtg
    11401 ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca
    11461 gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattactgac cttgccgaag
    11521 gggtcaaaac gttggcactc atgctttatc aacttgcctg gcaggaataa ggagtcataa
    11581 atgggatatt taaataacgt caccggttac cgcgaagatt tactggctaa ccgtgcgatt
    11641 gttaaacacg gtaatttcgc actgttaacc ccggatggtc tgataaaaaa tattattccg
    11701 gactttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt
    11761 gattatctgg tcacactgca tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc
    11821 attgaaacgt tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca
    11881 tttgccttaa gcgaaggtgg ctatctttat tgcccaccag gctccttaat gacgtttgtt
    11941 aacgcccagg ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa
    12001 ggccatgcac cgtggctgtt ttctggcaat gccagcgaac tggaacgcat tcactatgaa
    12061 ggtatggatg atgtgatcct gctggatttt ctgcccaaag agttaggttt tgatatgaac
    12121 atgcatatcc tctcttttgc accaggtgcc agccacggtt atatcgaaac acacgttcag
    12181 gaacatggtg cctatattct ttccggtcag ggggtttata
//
LOCUS       DAZXUK010000111        12112 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZXUK010000111 DAZXUK010000000
VERSION     DAZXUK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12112
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.111"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..370
                     /locus_tag="ACH3L8_002376"
     CDS             <1..370
                     /locus_tag="ACH3L8_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085457.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="ACH3L8_002377"
     CDS             complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="ACH3L8_002377"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFT4085458.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="ACH3L8_002378"
     CDS             complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="ACH3L8_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFT4085459.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="ACH3L8_002379"
     CDS             complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="ACH3L8_002379"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFT4085460.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="ACH3L8_002380"
     CDS             complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="ACH3L8_002380"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFT4085461.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3448..5049)
                     /gene="tap"
                     /locus_tag="ACH3L8_002381"
     CDS             complement(3448..5049)
                     /gene="tap"
                     /locus_tag="ACH3L8_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFT4085462.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5095..6756)
                     /gene="tar"
                     /locus_tag="ACH3L8_002382"
     CDS             complement(5095..6756)
                     /gene="tar"
                     /locus_tag="ACH3L8_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFT4085463.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="ACH3L8_002383"
     CDS             complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="ACH3L8_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFT4085464.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="ACH3L8_002384"
     CDS             complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="ACH3L8_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFT4085465.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9394..10320)
                     /gene="motB"
                     /locus_tag="ACH3L8_002385"
     CDS             complement(9394..10320)
                     /gene="motB"
                     /locus_tag="ACH3L8_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFT4085466.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10317..11204)
                     /gene="motA"
                     /locus_tag="ACH3L8_002386"
     CDS             complement(10317..11204)
                     /gene="motA"
                     /locus_tag="ACH3L8_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFT4085467.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="ACH3L8_002387"
     CDS             complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="ACH3L8_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFT4085468.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11912..>12112)
                     /locus_tag="ACH3L8_002388"
     CDS             complement(11912..>12112)
                     /locus_tag="ACH3L8_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFT4085469.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2782 a         3388 c         2934 g         3008 t
ORIGIN      
        1 cactacgcta atgagttggg atgccgtgct ctatgatcta cgtagtgaaa aaattatcag
       61 taaagataat tacttgaccg acttaaaccg cagacatctt gaacttgttc ttcatactaa
      121 ccccaatcca agcggttcgg taacgaagat tttacgcact atttttacta agcaggtaaa
      181 gagtttaggg ccggaactct gtcatttctt gcaccatgaa ctgacgaaaa aacgttatga
      241 agtatggcat cactacgatg tactgcacta ccagtcatcg tcattttctc gttcacaatt
      301 taatgcattg ataaatttac tggcgcaaca atcacgcttc gctgatctaa aattatcaaa
      361 ctacaaataa ccatcctcca cgtactccct gaacgatacc cgccaccctg ctatcggctt
      421 ggcatcatcg cgcaggccga agttcaggca atacaaatca aaatccaaga ctatccaaca
      481 aatcgtccac ctgatcctga ctggctacca cgccggcttt gctggtatcg acctgaggtc
      541 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
      601 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
      661 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
      721 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
      781 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
      841 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
      901 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
      961 tggcttccgc cgcttcggca atggcctgaa ccagccccag ttcccgcaaa ctgtcgcgca
     1021 gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     1081 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     1141 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     1201 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     1261 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     1321 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     1381 tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     1441 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     1501 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt gccaacattt
     1561 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     1621 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     1681 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     1741 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     1801 gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca ctacgcgcca
     1861 gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaagaca cgttctccgt
     1921 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     1981 tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc ggcaacggtt
     2041 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     2101 cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc ttatgtgctg
     2161 caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta tacgccagca
     2221 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     2281 cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca acgggcattg
     2341 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
     2401 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc agcggatcag
     2461 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
     2521 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
     2581 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
     2641 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
     2701 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
     2761 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
     2821 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
     2881 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
     2941 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcac
     3001 ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
     3061 cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg cgagcagagg
     3121 gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
     3181 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
     3241 cagtcccaga gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
     3301 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
     3361 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
     3421 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
     3481 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
     3541 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgagga
     3601 gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct caatcccccg
     3661 ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
     3721 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
     3781 acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg cgctacggct
     3841 ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
     3901 agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
     3961 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
     4021 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgccagttc
     4081 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
     4141 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
     4201 gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac gcacatcact
     4261 caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
     4321 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
     4381 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
     4441 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
     4501 acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc
     4561 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
     4621 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
     4681 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
     4741 acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt
     4801 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgcggtact
     4861 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
     4921 gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
     4981 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
     5041 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa ctcgtcaaaa
     5101 tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg gtggttgctc
     5161 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa
     5221 cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg cggcggcagc
     5281 tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa
     5341 tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
     5401 gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac
     5461 cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt tgatctcttt
     5521 tgccgccagg gcgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc
     5581 acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt
     5641 ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc
     5701 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
     5761 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
     5821 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt
     5881 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
     5941 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
     6001 ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
     6061 accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca acatacggcg
     6121 aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
     6181 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
     6241 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
     6301 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagttaacgc
     6361 tgtgtaatag tttttatatt tttcatcaaa attacggctg gtagcgacca tttcaggtaa
     6421 cggtgccatg cttttgaatt ttttataatg cgtcgcggcc tgggccaatg ttttcctggc
     6481 gctatcgagc aattcaactt tggcgttact ttgttgattg gaggaatcca tcatcatccg
     6541 caccgctgaa cgactcaggt taatgcgcgt ttgcagcatt aaatcccagg ttgacgtcag
     6601 ctcgccctgc tgttcccgta attgattgga aaccacaaag ctcttctggc tatggtgaag
     6661 ggaagaaaaa aacaaactgc cggaaataag ctgtaacagt gcgaataccc ccagcaccat
     6721 taccaacagc gtgactacgc ggatacggtt aatcataagg caccttcctg aaaacaagtt
     6781 gatctcgtta tcggcaagaa ggggggaaac tttattgctg atgccacccg ccgcgaaatt
     6841 gaaataaaaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa atcgggagaa
     6901 ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt tcagcagttt
     6961 ttcgatgttc accagaatca acatacggtc gcccagtgcg cccagtccag tgagatattc
     7021 tgttgaaagc gtcacggcaa actctggtgc cggacgaatt tgctccgccg tcaatgaaag
     7081 cacgtctgaa acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat tcaggacgat
     7141 aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc gtaagtcaac
     7201 aatcggcaca ataacgccgc gcagattcgt gacgcctttg ataaacgctg gcgtgttcgc
     7261 aatccgtgtt acctgatcgt agccacggat ctcctgcact ttcaggatat caataccgta
     7321 ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc tggccagctt
     7381 tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc ggcggtgttc
     7441 gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga catcaacaat cagtgccacg
     7501 ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata gttactttca
     7561 aggtttttaa ccacaacctg gtgttgacca attaattgat ccaccagcaa ggcgtagcgg
     7621 cgaccgccac tttgtaagat caccacaatt ccctgggtgg cttcggtttt cgcgcccgcg
     7681 acgttgaaca ctttccacag ttcgacgatg ggcagatatt caccccgcac ttccagcacc
     7741 cgctcgccgc cggccagtgg atggagatcg gcttcacggg gttgcagtga ttccataaca
     7801 gcattcagcg gcagaatgaa aacttcatcc gcaacgcgta cggacatgcc gtcgaggatg
     7861 gccagcgtca gcggcagtaa aatgcggatc gtagtgccag taccctgctt cgactggatt
     7921 tcgacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc gacgccgcgc
     7981 ccggagacgt cggtgacctg ctctgccgtg gagaagcctg gtgcaaatat cagcattgca
     8041 acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt gcgaggccgc ttttgccaga
     8101 attcgctcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat gttgccgccc
     8161 tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcggc gagccgtttt
     8221 tctggcagtt caataccgtg atcgaggcta ttgcgtacca ggtgggtcag cgggtcgata
     8281 atgcgttcta tcaggctctt gtcgagctcg gtggaactgc ccaccagcgt cagttctacc
     8341 tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact aaagacatat
     8401 tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgggc gttacgttgt
     8461 aactgcccca tgctggtaat caaatcacca tgattaaccg ggtccagttc gctggaacgc
     8521 tgggcaagca tggactgggt gataaccagc tcgccgacga ggttaattaa ttgatcaacc
     8581 ttttctaccg ctacacggat gctggtggat tcactgctgc gcgtcgtttt ttcccgctcc
     8641 acgcggccgg ttggcgcttg ttcggctgcc agtttaagca caggtggggt agctattttt
     8701 ggcgaaactt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa acagagtacc
     8761 gctgtgatgt catcttcggc gatatcgccc ggtaatattg ccgagagaga atccgcccct
     8821 ttcaccacgt catttaacgt tgtcagatgc cccagctctt cttccagcag gtcgacttcg
     8881 ccggccttca ggcgcgaaag gataattcgt cgcggcaact gactgcgact ttgctcatct
     8941 tgcggttcac ttttggcaac cacacttaat cgggtcactg cggatggcgt ttcgcctttc
     9001 gcttctaatg ccagttgacg caaggcctgg cagatataat cgtagctggc ggcatccggc
     9061 tcttgcgact gtttataagc gtcgagctgt tcttgcatga tgtccttcgt ttccaaaaac
     9121 agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc gagcaggttt
     9181 tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa aagttcctgc ccctcctttg
     9241 atcgagtggg cagcccgaaa gatggcattc aattgttcgg catctggcgc ttccggctgc
     9301 aaaaccagca gatgctgctc catatcagcc aacagttcgt ccgcttcatc aaaaaatgtc
     9361 tgataaaaat cgcttatatc gatgctcacg ctgtcacctc ggttcggctg atggcattgt
     9421 gggaacactg acctgtggtg caacctcagg tttttctagg gcgcttactg gctcattctg
     9481 gctttcggcg ttttcatgca aaatggcctg ttcggcttgt ttgttcagta ccaacaggct
     9541 gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg ttgccgccat
     9601 gccgacgaca cgtaacactt tgccgctatc caaccctccg accatcagtt cgcggcggga
     9661 tgcattggcc cgatcggcag aaagctccca gttgctgtaa cctttctcac cgctggcgta
     9721 gggaaaatca tcggtatgac ctgaaaggct aatacggttg ggaataccgt tcagcacagg
     9781 cgcgatggcg cgcagaatgt cgcgcatata gggttcgaca tcggcactgc cggttctaaa
     9841 catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga ccagatcgat
     9901 tttgagatgg agacgtaacg cccgcagttt cggatcggac tctatcaact ggtcgagatc
     9961 accccgcaat ttccgcaatc gactttgctc catgcgtttt ttcagctctt cgatgttcgg
    10021 ctgcttattc acttccccct ggctttgggt gtaatcatca ccaccgccgg gaattgggct
    10081 ttcactattg gaaatgcgat cgccgcccgt aaccgctgtc gccagtggag tccggaagta
    10141 ctccgcaatc tgaatcaact cttttgggct ggagatggag atcagccaca tcaccagaaa
    10201 aaaggccatc atcgcagtca taaagtcggc ataagcaatc ttccacgatc catgtgctgc
    10261 cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt gattcttcat
    10321 gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg cacgcacatg ctcttccagt
    10381 tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc aacggcgata
    10441 ggcggtgcgt aaccgttcag attagaaagc agagtgactt tgacgcactg catcattttg
    10501 ctggtttcgg cgcttttctg acgtaaaaca ctcgctagtg gggaaataaa tccgtaagcc
    10561 aataaaatac cgaggaaagt ccccaccatc gcatgtgcga taagcgcccc cagctcggcg
    10621 gcaggacgat cggctgaacc taacgcgtga acgcccccca ttacagccgc gacaatacca
    10681 aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc tgcctcgctt
    10741 tcgtgcgtct caatctcttc atccatcagg gcttcgattt cgaaggtgtt catgtgaccg
    10801 ctgataatca ggcgcagata atcgacgata aaatcaagca tgacgctatc cgcgaggatg
    10861 cgtgggtagc tggcgaagat ctcgctctca cggggatttt caatatcacg ttccagcgaa
    10921 aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag cagatccata
    10981 tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcttt cagcgtgcct
    11041 ttaatcgctt tgccattatt gccgacgata aacgacccaa tccctgcacc ggcaataatc
    11101 accagttcag cgggttgata gagtgctcca aggcttccac cggtcatcaa ataaccgccg
    11161 aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc atccttccac
    11221 tgttgaccat gacaggatgt tcagtcgtca ggcgttaacg cgcgattggg gaaaaaaaaa
    11281 gcagcggtac gtcgttaccg ctgctggaat gttgcgccac accgtatcag ttaaacagcc
    11341 tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg ggaaagttta
    11401 cgtcttttta ctgcccggga tggcggttga cataagctgc aggcaaagct gccaacaggc
    11461 tggtgagcgt gggtaataaa attgccgccg cagcagttgc agctggaaag ttgcagtaat
    11521 ccactttcaa caaaccgcac caatgtccag gcacgggtta atgccagcag tggtccttct
    11581 tctgcttgtg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc atcgacgcca
    11641 ttacacaaac cggttttcag taaaaactgc catgcattac agaacatcga agcatgaacg
    11701 ttttgttccc aggtcataaa ccagtcggtt gagaatggca gcatgccttt cggcggtggg
    11761 cttccgcgca gttctttata aagttttatc aggcgtccgc gacttaactg tgtttcgctt
    11821 tccagcatct gcaaacgagc gcccagggtg atcaattcca ttgccagctg aatatcccgc
    11881 gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttgcgc agcgcttctt
    11941 caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta tgaatttgct
    12001 ggagatcgtc aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg ctgtcaaaac
    12061 ggaagtgaca aaccagctga ttggtttctg ccagcttaac catttgcgga ag
//
LOCUS       DAZXUK010000112        12001 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZXUK010000112 DAZXUK010000000
VERSION     DAZXUK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12001
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.112"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..2250)
                     /locus_tag="ACH3L8_002389"
     CDS             complement(<1..2250)
                     /locus_tag="ACH3L8_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HFT4085470.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTEGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFRDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTLYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVE"
     gene            complement(2274..2897)
                     /locus_tag="ACH3L8_002390"
     CDS             complement(2274..2897)
                     /locus_tag="ACH3L8_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFT4085471.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2894..4582)
                     /locus_tag="ACH3L8_002391"
                     /pseudo
     CDS             complement(2894..4582)
                     /locus_tag="ACH3L8_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4731..7358)
                     /gene="gyrA"
                     /locus_tag="ACH3L8_002392"
     CDS             complement(4731..7358)
                     /gene="gyrA"
                     /locus_tag="ACH3L8_002392"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFT4085472.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7505..8227
                     /gene="ubiG"
                     /locus_tag="ACH3L8_002393"
     CDS             7505..8227
                     /gene="ubiG"
                     /locus_tag="ACH3L8_002393"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFT4085473.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8355..11930)
                     /gene="yfaL"
                     /locus_tag="ACH3L8_002394"
                     /pseudo
     CDS             complement(8355..11930)
                     /gene="yfaL"
                     /locus_tag="ACH3L8_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2779 a         3325 c         2957 g         2940 t
ORIGIN      
        1 ttcgacattg accagttcac caggctggta atgggttttg tccgttttca cccggatatc
       61 aagctgagga acggcaactt tgatcccggc gttctgaaaa ctgtactgac cattacgggt
      121 atacagcacc gaaaaagtga tgttaggcgc aaaggaattg ctcactggta cccgggcttc
      181 atacagggta tcgtttaaac gttgtagcgt tagccagttt gccggatgcg aaagcagcga
      241 ctgctgttcc acgcgatcgc gttccagcgt caataacgct tcatcaattg gctccgggaa
      301 agtaatcagc atcttcgcgg tttcgcctgg ctggtacagc gttttatccg ccacgatatc
      361 taccgtacca gtatgcgccg tgctgccctt accgctgacg gcatgactta acccagcgag
      421 aattaagccg tctttatcgc gtaatgtcag attgtagttg ccaggtttag cgaaattgac
      481 ggtaaaggat ttgccgcctg acggtagctc tccgctatgg ctcgtgcggt cttcgagacg
      541 caaccattca tacgtaacag gaacctgttt tgaagattcc agcgcggcat aacggaacac
      601 aaccgactcg ccgctattac tgtattgtgc ggcggtactt aaagagtaat gcgccaggcc
      661 gcgttcaatg aggatctctt tggtggtggt gacgcgatac gccgcgccgt cactggcgga
      721 gacggttaac aaatagcggc tcggtttatc ggcggcgggg agatttaacg ccacatgacc
      781 gctggcgtcg gacaccgttt cgctgccttc cagcgacacg gggaaacgtc cggcataacg
      841 caaatcgtta ccgaccattg ataattgctg agcgcgcaaa cttaactgca cgcgggcatt
      901 ttttaccggc tcgccatccg ggtagagcag ttgcagtttg ccgctgaccg cttcgccagt
      961 tttgaactct tttttgtcga gagctaaacc aatctcgaaa tgtggcttga tgtagtttgc
     1021 cacgcgaaaa ctgctgctat agacctgatt gcggtaagca agacgtaact cataacctcc
     1081 ggcaacggca ttttctggca ggcggaaact tccctgcccg ccattgcgcg catccagcgt
     1141 gacatcgacg gtttgcaaca gactgccgtt agcgtccagc accgaaagct tcgccggggc
     1201 gctgacgatg ggggatgaat gcaacggatc gcggaactcg cggcccatca ctttaacatc
     1261 gacacgatcg cctgcgcgat atagcgggcg atcggtaaaa atatacaagc gggtgttgta
     1321 gatttcgctt tcgtagaaga agttctcgga gacaaaaacg ccgccttcag catccttacc
     1381 cagaatgtat gaacgttctg gcgatatatg ttgtaactgc aaagtaccgc tgtcatcggt
     1441 caccccgcgg gtcatcacgc caagaccttc agtccacaag atctctgagc cgggcttcgc
     1501 ttcaccctgt tttttacccg cggtccatac cagaagctcg ttacctgaca ctttgctaag
     1561 cgccacggta tcggaaacaa acaccaccgt cgtcgcccga tacccaccta ccatcgcctc
     1621 gacgaggtac agccccggct cttgttggcc gagaggaata taaatattac ccggctgcgg
     1681 cgagatgaaa ttgctggatg cgccttccag ttttacccct tgctgcggct cgaccggttt
     1741 agcctgccat agtggataac ggaactgttc caccaaggga tattttttca gcggggaaaa
     1801 ctggttgttc tgtacataac gggaaggttt aataatggca ttgccgagat gtaattcggg
     1861 taatgcctga gtcacattct gccgtgactg agaagagaaa gtacgctgca tcacgcggcg
     1921 agatttgccg taccagttat cccacagcca ggtcagcgta ttgttcagcc cgtcgcccag
     1981 atattgcggt tgcaccacaa tgcgatgcag gtttttctgc tggcgcaaaa atgccatcgg
     2041 gtcaggaata cgatataggc gaacgtccac gccgccgtac tcttccatct gatagcgccg
     2101 ataatcacgc cccggcgctt ccagtcgcac tttcgcctct tcactgctgc taaaactgct
     2161 gtcagcaagc aaaaagaatg ttcccccggc gggcggcgca tagttactgg aaggaagcga
     2221 atcatcagct ttagcaagcc ctgttcccac aagagacagg gacaagcacg ctgctatcgc
     2281 gccagaaaat ttaaacgata aatgccaatg aaattgggat tggaatcgtt gggtatccat
     2341 cgggtgtcct tccatgtcat aagttgttgc agactgactg cgcgcattcc gttgtcagtt
     2401 ttcgtggcgc ttccggtgtg gtagatgacg taacgcccca tccagaccat taagtgctgg
     2461 gcatcgccct gatcgaaaaa aatcatatcg ccaggcagcg cctggtttat gtcttggcca
     2521 ataaactggc tgttgtactg aatcaaatta atcgcggtca cgtaggggcc gtttttcccg
     2581 ctcccctgat tccagttttg cgccagttga cgctgttcag gtgttagcgt catctctggc
     2641 ggcaaatact ggctagataa accgttactt tttagccatt tactgtcgtg aactttcagc
     2701 gtctcgttcg ccgcaaatcg caccaggccc gcacaatcct gctgatacca gcgtggactt
     2761 ggcccctggc ggagctgttc ttgtgcaatg cgtacaaacc aggcgcgaaa cagcctggat
     2821 tgttcgacat tcagcatttc gctatgggca acaacacaac acagccagca aatcagcgcc
     2881 agcagcccgt gtctcatagc ggctgccagg ttatgggtag ccactgccag gcgacaccgg
     2941 gttccatctg ggccagcttc atgacataac gcggttgttg agataaagcg tccagcttcg
     3001 gcattaataa agtttgtgcg gcgttataaa aaaccggttc gagattcttc ggcagactgg
     3061 tcaacgtttc gttacgcagc agtttcgcca cgccttgtgg attgatatag agcggaacga
     3121 tgccatcagt gggtattaca tccaccattg ccgggcgggt tttattcagt gtttgcaggg
     3181 cgttattaac cagcgtgtca tcgagggaga aaagcagcgt tttgttttgc atcgccagcg
     3241 acacacggaa aaaataatcc gacattaatt gatcgggttg cgccgcctgc gctttcggat
     3301 actgaccgta tcgggaactc acttcgcgac accagatttg cgcttcgccc tgctgagtct
     3361 ggcttaccgg caaaacacct tctggcgctt tgctttcgtg cgcaccaata ttttgcgtaa
     3421 acagtttccc tggcaattgc gcctgttcgg cagtgccatc aaactgaccg acaaacagcg
     3481 gggtttgcaa ttttgagtct tcataccagc acagccccgc ggcaccgtct aacgccccat
     3541 tcagcttgtc gttttcctgg ctgatgtgcg aaagcatctc ttcggcaata ccgtgtgaat
     3601 acggcaccgc cacacagaag ctggctccgg caggcatact gttccagacc ggcgtaaaat
     3661 cgaaactggc atctacgctg gcggattcat catttaacgc cacaaaactg tgccagccgt
     3721 cgttacccat ttcgaagcgt acgccagcaa aagaaggcat taaccgctgg tagccaaaac
     3781 ccagcaacct ggcgctgacc acgatgcgca ggcgtactgg cgttttttcg gctgtacgct
     3841 cttccaggcc aaagctggct tgccagcgtt ttttaccgct caacaaatca cttgcgatcg
     3901 ccgtggcttc ggtatcctgc tgatcatctt taaacaacat atccgtactg gaaaacacca
     3961 gcatcttgtc ctgatatgtc gcgaacatca gggcgttatt gccgttatag cgcaactgat
     4021 aaacaggaat agtttcacta tttatcttga tgctactgat ttccgttttg cttaactagc
     4081 tgtcgctggt agcggcaaac aacaatggct ccagcagttt gcttaaacca ctgcgctgga
     4141 tcagcaccat gtaatgtgaa agatggcctt gtttatcgtg ccatagcgcc gcctgcgcgg
     4201 gctgatctaa gagtgacgaa aagagcttat ctttcagcgt gagatcgtgt tcatagacaa
     4261 tgcggcgaat gctgccttca atgcccagac gatcggcatg attctgataa tagaaaacga
     4321 aatcttcgct cagaacatcg tggagaaacg gaatggtgag gagatctttg ggaagctggc
     4381 tcagagagtc gctgtctaga aagaggtccg gctcattgag atcgatttgc agattgttgt
     4441 gcaccaccag cggcgacaac gttttttctg gcccactgcc agcatattgc aacgcccaga
     4501 cgccagcgga aagcagtgct attgcgccaa aacctacaag accatagaac cgccagcctt
     4561 tcgccttttt ttcaccactc attgccacat tccttgtgta tagccagcca ttttttacgg
     4621 gcacagccaa actttaccgc gccctaatac gacaaaagcc cagactttgc agcctggact
     4681 tttcaattca aacaagggag atagctccct tttggcatga agaagtaaaa ttattcttct
     4741 tctggctcgt cgtcaacgtc cacttccgga gcaatttcat catccccttc cgcggcactg
     4801 ccgtcgatgg tatccagatc ttcctcgtca accggttcag caacacgctg cagacccact
     4861 acgttttcat cttccgcagt acggatgagg atcacgccct gggtgttacg gcccacgatg
     4921 ctgatttctg aaacgcgagt acgtaccagc gtaccggcat cggtgatcat catgatctgg
     4981 tcgcagtcat ctacctgcac cgcgccaacc actaaaccgt tacgttcggt aaccttgatg
     5041 gagataaccc ctttcgtcgc acgcgacttg gttgggtatt ccgccactgc agtacgttta
     5101 ccgtaaccgt tttgcgttgc ggtgaggatt gcgccatcgc cacgaggcac gatcagagag
     5161 acgactttat cgccttcacc taagcgaata ccgcgcacac cagtggtatt gcagcccatc
     5221 gcacggacag aagactcttt aaagcgcacc actttacctt cggcggagaa cagcattact
     5281 tcatcttcgc cactggtcag atcaacgccg atcagctcat cgccgtcaac cagtttgatc
     5341 gccactttac cggcggtacg cagacggttg aattcggtga ggacggtttt cttcacagta
     5401 ccgttagcgg tcgccatgaa gactttcacg ccttcttcaa actcggtcac cggcaggatc
     5461 gcggtgatac gttcgtcctg ttccagcggt agcaggttga cgatcggacg accgcgcgcg
     5521 ccacgagtgg cttccggcaa ctggtaaact ttcatcgaat agacgcgacc acggctggag
     5581 aagcacagaa tatggtcgtg cgtgttcgcc accagcagtc ggtcgataaa gtcttcttct
     5641 ttaatacgtg cggcagattt acctttcccg ccacgacgct gcgcttcgta ttcagaaagc
     5701 ggctgatatt taacgtagcc ctggtgagag agcgtcacga ccacatcttc ctgggtgatc
     5761 agatcttcca ggttgatgtc tgcgctgttg gcggtgattt cagtacgacg tttgtcaccg
     5821 aactgttcac gaaccagctc cagctcttcg cggatcactt ccatcagacg atcggcgcta
     5881 ccaagaatac gcaacagttc cgcgatctga tccagcagct ctttgtattc gtcgagcagt
     5941 ttttcgtgct caaggccggt cagtttctgc aaacgcagat ccagaatcgc ctgagcttgc
     6001 tgttcggtca ggtagtacag accatcacgc acgccgaact ctggctccag ccattccgga
     6061 cgcgcagcat cgtcgccagc acgttccaac atggcggcaa cgttgcccag ctgccacgga
     6121 ttagcaacca gcgcagtttt cgcttctgca ggcgtcggcg catgacggat cagttcgatg
     6181 atcgggtcaa tgttcgccag cgccacggct aatgcttcaa ggatatgagc acgatcgcga
     6241 gctttacgca gttcgaaaat agtacgacgg gtcaccactt cacggcggtg acgaacaaac
     6301 gccgcgatga tgtctttcag gttcatgatc ttcggctgac catggtgcaa tgccaccatg
     6361 ttgataccga aagaaacctg caactgggtc tgggagtaga ggttgttgag cacaacttca
     6421 ccgaccgcat cgcgtttcac ttcaatcacg atgcgcatac cgtctttgtc agactcgtca
     6481 cgcagcgcgc tgatgccttc cacgcgtttt tcttttacca gttccgcaat cttctcgatc
     6541 aggcgcgctt tgtttacctg atacggaatt tcgtggacga taatggtttc acgtccggtt
     6601 ttggcgtcaa cttccacttc agcacgggcg cggatatata ccttgccgcg accggtacgg
     6661 taagcttctt caataccgcg acgaccgtta atgattgccg ccgtcgggaa gtccggcccc
     6721 gggatgtgtt ccatcagccc ttcaatgctg atgtcttcat catcgatata cgccagacaa
     6781 ccgttgatga cttccgtcag gttgtgcggc gggatgttgg ttgccatacc tacggcgata
     6841 ccggaagaac cgttcaccag caggttagga attttggttg gcatgacgtc gggaattttt
     6901 tccgtgccgt catagttatc aacgaaatcg accgtctctt tttcgagatc ggccatcagt
     6961 tcatgggcaa ttttcgccag acggatttcc gtataacgca ttgccgccgc agagtcgccg
     7021 tcgatggaac cgaagttacc ctgaccgtct accagcatgt aacgcagcga gaatggctgc
     7081 gccatacgga cgatcgtgtc ataaaccgcc gagtcaccat ggggatggta tttaccgatt
     7141 acgtcaccaa cgacacgggc agatttttta taggctttgt tccagtcatt gcctagtacg
     7201 ttcatggcgt aaagtacgcg acggtgtacc ggcttcaggc catctcggac atctggcagc
     7261 gcacggccaa caatgaccga catcgcataa tccagatagg agctctttag ctcttcctca
     7321 atgttgaccg gtgtaatttc tctcgcaagg tcgctcatct aaccgctatc cctctactgt
     7381 atcccggatt caaaggtcgc aaattataac acagccgcgc agtttgaggt aaacctatac
     7441 gctttattca catccaatgc ctgatatact cgtttgtctt gccaattacg gagtagaagt
     7501 gccaatgaat gccgaaaaat cgccggaaaa ccataacgta gaccacgaag agatcgctaa
     7561 atttgaagcc gtcgcctccc gctggtggga tcttgaaggt gagttcaaac cgttgcaccg
     7621 cattaacccg ctgcgtctgg gctatattgc cgagcgtgct ggcggtttat ttggcaaaaa
     7681 ggtgctcgat gtcggttgtg gcggcggcat tctggccgag agtatggcgc gcgaaggcgc
     7741 gacggtgacc ggtctggata tgggctttga gccattgcag gtggcaaagc tgcacgcgct
     7801 ggaaagcggc attcaggtgg attacgtgca ggaaaccgtg gaagagcacg cggcaaaaca
     7861 tgccgggcag tatgatgtgg tgacctgcat ggagatgctg gagcacgtac ccgatccgca
     7921 gtcagtggtc agagcctgtg cgcaactggt gaaaccaggc ggcgatgtct ttttctcgac
     7981 gcttaaccgc aacggcaagt catggctgat ggcggtggtt ggcgcggaat atattttgcg
     8041 catggtgccc aaaggtacgc atgatgtgaa gaagtttatt aaaccggcag agctgcttgg
     8101 ctgggtggat cagaccagtt taaaagagcg acatataacc gggctacatt acaacccgat
     8161 cactaattcc tttaagctcg gccccggcgt ggatgtgaac tatatgctgc acacgcagaa
     8221 taagtgaggt tgatgtttgg ccgcgccaat gcctgatgcg acgcttgcag cgtcttatca
     8281 ggcctacaaa tgctctccgt aggccagata aggcgtttac gccgcatccg gcaattgtga
     8341 tggtcagaca gcgattacca tttcaccgtc atcgacaaaa accctgccgt ctgggcaaaa
     8401 tcatcactcc ctttctgcca cgccacgctg ccgcgcaggg acactcgctg actgatattg
     8461 cccgtgactc ccacttttat ttcaccccgt agcttcaccg catcgtcact gatagtgctg
     8521 ccatcttctt caatttccgt cgcatgggga tcgtgatagt aattcagatc taatgttggt
     8581 gtgacaccaa ccgcggtacg ccattcgctg tgtaaaccca gccgcgtctg aatatcatcc
     8641 ccctgcgatt gtgacacgcg cgcatggtta gcggcggtaa aatcatcctg ctgcacgccc
     8701 tgataaatca cctgcgcctg cggttcaatc accacaccac gccccggtaa ccactgatac
     8761 ccagcttcca gcgaggcgat aatccccgac gaatggtaac gatccacgcc atcttcatgt
     8821 tcggaaacat cattgttaaa ccacgcgtat tgcagccagc tatccagcca ggccccctgc
     8881 ttctgattac cgtgctgata ccagcttgag gtcagcccaa cggcataacc gtggttctgg
     8941 ttatcggcgc gagttccggt catattcgag cggctgtcgc cctgattatc actgtagcca
     9001 ccaaccgccc caagcatcca ctcgccatcc gtgccccagc gcccgctaaa caagccgccg
     9061 ctaagctgca ccgtagaagt gtcttcctgc tgagccagtt gccctgccgc cgtgtaatga
     9121 taacgtccgc cgataacacg taaattcagc gtctgaccat cgccaccagc gtgatcgcgt
     9181 cgctccatca taaacgcctg atttgccgcc cgcaggttat tgagataacc gccaactttg
     9241 gcattcagca ccggctggta agcaggcgta ggggccgggt cgggggttgg gtcaggatcc
     9301 ggcgtgggat caggatcggg agtcgggtct ggctcaggtg gcgatggcgg cgttacttct
     9361 tgcgatcgca gataccagtc gttgttatct tccactagcg tgtagtcata cgctcccatg
     9421 ttgacgtagc cactgccagc cagactgaac tgtgcattgt tttgaaactg cgtgggatca
     9481 gctgcgaaat caaccacttt aatgcctgtc gatgtcggct caccaatccc tgtaatggag
     9541 ttaaccacca cagttgtgtt gccagcagta ttaccgttca tcaccaattg atcgcttacc
     9601 gagtcatcgc cgtttaattc gctgtcgaat agtagcgtac cgccgccggt ataatcaccg
     9661 tttaccgtca gcgtatcgcc agcgacgcca ttttgcaggc taaccgcacc ggacgtattg
     9721 gtcagatcac cattaaccgt cacgtcagca agataggtca ggctgtcagt ttcaaggttg
     9781 gtattaagct cgcctgtcaa cgtcacgcca tcggtagcat ttaccagcgt cccatcgccg
     9841 ttaaatagtg aggcattaag ggcgacggaa gtatcctgcc cggtcagacg caaaaccgta
     9901 ccatcgctga tgtcgatagt gccgctggaa gtagcatcaa ttgactgaat atcctgatcc
     9961 gcgccagtaa cgaacgttgc cccatcacca acccacagtg acgaagcata aggaaagata
    10021 tccgcaacat cgcctttcag ggtgccttct tcgacacgta ccgccgactg cgttgtaccg
    10081 ctggcggtca gctccagcgt acccgccccc gttttagtaa atgtgctacc ctcatcctga
    10141 tgctgcccgc tgctgtcagc catcagtgcg ccccactgcg tgtctacccc cgcatcaact
    10201 gccacttcac cgtcggcgcg catttcaata tcacgcccat gctcagtcgt cgtagagcga
    10261 gtatcgacat tggcagtaat ctccatcacg ctttcttgtt acttatcggt aaagataacc
    10321 tggcggttat agtgggtatc tccaagctgc gagttgtcgc tcaccatcag cgtaccggag
    10381 gcgatttgtg ttgtgcccaa ataactatta ttgtttgcca gacttacctg cccgtcgctc
    10441 gcatcgacca ccacgtagac gccatctttc ccgtcgccgc cgatatgcaa attgtcgcca
    10501 cctgcaagat ctaccacatc ctgactgccg ataaccgttc cgctgctgcc actgacttca
    10561 aggccatcat tgtatgatgt gccgctctgc caagtggaga aatcagagag atcgagcaca
    10621 ccgccgttta acacgatcga ctgcggatcg tcctggagag cggtaagatc tgccgcgtcg
    10681 ccttccagcg aaagcaccgc accatcatca acgactatat cgccggttag cgccatcgac
    10741 tgcgcccctg ccagcacgta gctgccgttt tgcgcaatgg tgagctgacc agcaccttcg
    10801 atgatgccag caaaactgcc ctgattaaca gtaacgttgc caccagcatc gatatttaaa
    10861 gtgccattct gaaagcccgt caatgcgtgc acaaaagttt gttgggtcga gccaacgttt
    10921 agctcagcct gattctgata ctgatcaata ctccctatcg tcagaccgta gcagtcttgc
    10981 ggatcgtcct ggcaatgcgt atcgccgaca ttcatcaggg agttgctgcg acccagggta
    11041 acttcaccgt tttcaatctg catctcacca gtaaagtcat tgttatctgc attaagtacc
    11101 agatcgccgg aacctgtttt ggtgattaac ccggtaccag caatagagtc aacagctccg
    11161 tcattctctg tattgccgat aaccagcgtt tttccgtcgg caatatcaaa ggtaacttca
    11221 cttaagccga gatacataaa gccacccgcc gcagtggagg gaccatctcc atagcctgct
    11281 gcgctattgt tctcatcgac taacacgccg ccgttctggc tgtagctgtc atcaacagaa
    11341 atatcaataa gatagggagc cgtcgcgcta ttggtatata tcgccccgcc ataaccttcg
    11401 gctgtgttat ttgtaaaggc ggtgttattt attatcgtat aacctgaagg atgcttgctg
    11461 tcgctattat tatcggtaac atcgattgcc ccgccatcgc catcactgta acttgttgat
    11521 gtatatgcct ggttattgtt aaaaacatca tcacttaaat agacgtcatt attaatggta
    11581 taaattgcgc cacctttgcc atcattagcg atattattga tggcgttagt gacacgtaaa
    11641 tcgacggcac cagtatcatt agtaccagaa gaatagattg cgccaccata gccgcctgcg
    11701 acgttaccgg aaaaaataac atcagtaaga ttcagcgttg agttttcttt agcaaatatg
    11761 gccccgccgt tattatattc tcctgtaacg gtgttattgg caaacagagt cattccagtt
    11821 tcattttctg gtaatagtga aaattccgct ccttgttgca aaaatacggc cccaccgctg
    11881 gcattattgg tcatatccga aaaaaccagc cattgcccat cggcgataga ctggccccct
    11941 gaatctccag acaaccagta tcacttattt aagtgatagt cttaatacta gtttttagac
    12001 t
//
LOCUS       DAZXUK010000113        11933 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZXUK010000113 DAZXUK010000000
VERSION     DAZXUK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11933
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.113"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(139..843)
                     /locus_tag="ACH3L8_002395"
     CDS             complement(139..843)
                     /locus_tag="ACH3L8_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFT4085474.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(980..1432)
                     /gene="moaE"
                     /locus_tag="ACH3L8_002396"
     CDS             complement(980..1432)
                     /gene="moaE"
                     /locus_tag="ACH3L8_002396"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFT4085475.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1434..1679)
                     /gene="moaD"
                     /locus_tag="ACH3L8_002397"
     CDS             complement(1434..1679)
                     /gene="moaD"
                     /locus_tag="ACH3L8_002397"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFT4085476.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1672..2157)
                     /gene="moaC"
                     /locus_tag="ACH3L8_002398"
     CDS             complement(1672..2157)
                     /gene="moaC"
                     /locus_tag="ACH3L8_002398"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFT4085477.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2160..2672)
                     /gene="moaB"
                     /locus_tag="ACH3L8_002399"
     CDS             complement(2160..2672)
                     /gene="moaB"
                     /locus_tag="ACH3L8_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFT4085478.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2694..3683)
                     /gene="moaA"
                     /locus_tag="ACH3L8_002400"
     CDS             complement(2694..3683)
                     /gene="moaA"
                     /locus_tag="ACH3L8_002400"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFT4085479.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3671..3815)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4080..4988
                     /gene="yvcK"
                     /locus_tag="ACH3L8_002401"
     CDS             4080..4988
                     /gene="yvcK"
                     /locus_tag="ACH3L8_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFT4085480.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5180..7201)
                     /gene="uvrB"
                     /locus_tag="ACH3L8_002402"
     CDS             complement(5180..7201)
                     /gene="uvrB"
                     /locus_tag="ACH3L8_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFT4085481.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7780..8457)
                     /gene="bioD"
                     /locus_tag="ACH3L8_002403"
     CDS             complement(7780..8457)
                     /gene="bioD"
                     /locus_tag="ACH3L8_002403"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFT4085482.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8450..9205)
                     /gene="bioC"
                     /locus_tag="ACH3L8_002404"
     CDS             complement(8450..9205)
                     /gene="bioC"
                     /locus_tag="ACH3L8_002404"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFT4085483.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9192..10346)
                     /gene="bioF"
                     /locus_tag="ACH3L8_002405"
     CDS             complement(9192..10346)
                     /gene="bioF"
                     /locus_tag="ACH3L8_002405"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFT4085484.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10343..11383)
                     /gene="bioB"
                     /locus_tag="ACH3L8_002406"
     CDS             complement(10343..11383)
                     /gene="bioB"
                     /locus_tag="ACH3L8_002406"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFT4085485.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            11470..>11933
                     /locus_tag="ACH3L8_002407"
     CDS             11470..>11933
                     /locus_tag="ACH3L8_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFT4085486.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSV"
BASE COUNT         2746 a         3286 c         3043 g         2858 t
ORIGIN      
        1 aatacttttg tatttatgga tacggaaaac gattccgctc gctcataagg catattttat
       61 acctggtact tgttaagtgt cgtttttagt aagaaaaaaa tcccgacaca tggccgggat
      121 agtgatggtg aaaaagaatt aacggcggtt gccgaagatc cgtaacaaca tcaggaacag
      181 gttgatgaag tccagataca aggttaacgc accaagaatg gaatatttgc gcaggttcga
      241 cgtgtcgcgg gtatcaatct gctcacccat atttttcaat ttctgagtgt catacgccgt
      301 caatccgaca aagacaatca cgccgatgta ggtaactgcc cacatcaatg cttcgctttt
      361 cagccagaag ttgaccagcg atgccagcac aatgccgatt aacgccataa acagcatatt
      421 gccaaagcca cttaaatcgc gcttcgtggt gtaaccgtac aggctcattg cgccgaacat
      481 cccggcagta acgacgaaag tactggcgat agaagcagcg gtatagacaa tgaatatact
      541 ggaaagcgta agacccgtca gcgccgaata aagcataaag agcatcgtcg ttacacctgc
      601 gctcagcttt tgaatcatcg ctgataacac aataaccaat gctaattgcg cgatgatcag
      661 accgattaaa aagacacggt tagtgaacaa cagctccatc acggccgcgg aattagccgc
      721 ataccaggca acaaatgcgg tcagcaacaa gccaacggtc atccagccat agacttgcgc
      781 catataagtt tgcaagccag cccagggttg tacgattgaa tcagaacgtg ggaatctgtc
      841 catgacgatc tcctgaagat ataaggaata tcttaaggat actgcaaaat gatgaggctg
      901 tgcatcgacg cagcgtaaac gcatgtactg agcggtgaaa ttgccggacg cagcggtgcc
      961 ttatccggct aacaaaaaac taccagcgtt ttgccgcctg cagatcgctc tcccgggctt
     1021 caacccagcg gtcgccttcc ggcgtggctt cgcgcttcca gaacggtgcg cgggttttga
     1081 gataatccat aataaactgc ccggcttcaa acgcactgct gcgatgcgca ctggtgacac
     1141 cgacaaaaac gatttcatcg cccggccata attccccgat gcggtgaatc accgtgacgc
     1201 gccccagcgg ccagcggtta cgcgcttcat caacaatttc tgccagtgct ttttcagtca
     1261 tccccggata gtgttcgagg gttaatgctt tgacgctgtc gcccaggtta tggttgcgca
     1321 cctttccggt gaaggtgact accgcaccgt cttcgtcacg ctccgccagc cacgggtact
     1381 cttctcctac gctgaacggc tgcgggccga caacaatttt ggtttctgcc atcttaacct
     1441 ccggttaccg gcgggaagaa agctacttcg tcgccgtcag tcagcggatg gtcaaaactc
     1501 accagcgtct ggttgacggc agccagcaat ttgccatctt ccagcgccag cgcccagcga
     1561 tcgctctgcg cagccaggtg ctggcgtaac gcttcaacgg ttgggtaatc cgcagccact
     1621 tcggttgcat ctgttcccac caactcgcgc acctgggcga aaaaaagaac tttaatcatc
     1681 cgcttccacc ttaaagtcac ccgacttgcc gccgctcttc gccagcaaac gtaccggacc
     1741 aatcaccata tctttttgca ccgctttgca catgtcataa atggtcagcg ccgccacgga
     1801 ggccgcggtt aacgcttcca tttcgacacc ggttttcccg gtcaggcggc ataaggtttc
     1861 tatacgcacc cgattgtgct ccggctcggc ctgtaaattg acttcaactt tgctgagcat
     1921 cagcgggtga cacagcggga tcagatccca agtgcgtttt gccgcctgaa taccggcaat
     1981 acgcgcagtg gcaaatacgt cgcctttgtg gtggcgacca tcaataatca tcgccagcgt
     2041 ctcgctgcgc atggtgacaa aggcttccgc ccgcgcttca cgcacggttt ccgctttggc
     2101 ggagacatcc accatgtgcg cttcgccagc ggcgttgata tgggtcagtt gcgacatact
     2161 tatttcttca aatgtggatg gaaattacac ggacgcgtac gggcatccag ctgcggcgcg
     2221 atgatatttt cccatgcggt acggcacgct ttggtcgaac ccggcatggc gaaaatcagc
     2281 gttttgttag cgacgcccgc taccgcacga gattgcaacg tggaagtgcc aatctcttca
     2341 aacgacaaca tacggaacac ttcaccaaaa ccttcaacct cacggtcgaa caacggcagc
     2401 agcgcttcgg gagcctgatc accttccgtc aggccagtac cacccgtaat caataccact
     2461 tgtacatcgt cgctggcgat ccacgcagat acctgagcgc gaatagcgta gcggttttct
     2521 ttcacaatgg cttcatcgac aacgtgatgg cccgcttctt gcgccgaatc gcgcagatag
     2581 tgaccggagg tatcgtcttc ttcaccgcga cgattagaaa ccgtaagaat agcaatacgg
     2641 gtcgggataa attcagtgct tacctgactc atctgatctc tccttttgac gttttagccg
     2701 ccaatgtacg ataagttttg cgtaataccg gtgttgtttt gatgcaggaa atgggtctgt
     2761 ttcttctccc gcagcgccgc tgaaatacgc gcttccagcg cctgttgctg ggtatcgcct
     2821 tccagcagat cgcgcaggtt aacgccgcct tcaccaaaca ggcagagatg gagtttacca
     2881 atggaggaaa cgcgcaggcg gttgcaagtg gcgcagaagt ctttttcata cggcatgata
     2941 aggccaatct ctccggcgta atctggatgg caaaagactt gcgcgggacc gtcgctgcgt
     3001 tgacgtaatt ggtggatcca gccgcgacgc agtagctcgt cacgcagaac ctgaccagag
     3061 atgtgatgct tacggaagag ctcgctgccc tcgcccgttt ccatcagttc gatgaaacgc
     3121 agctggatag ggcgatgctg gatccagttc agaaaggtgt cgagctggtg atgattaaca
     3181 tcacgcatca gcacggtatt gactttgacc ttctcaaaac cggcctcaaa tgcagcatca
     3241 atccctgcca tgacctggtt gaatttatcc tgcccggtaa tagcgtgaaa ctggcgggcg
     3301 tccaaactgt cgacactgac gttaatgcca gtaagtcccg catcgcgcca gttcgccaca
     3361 tcgcgttcca gacggtaacc attggtggtg accgcaatct ggcggatagc gtcgttttcc
     3421 cgcacagcgg cgatgatatc ggtaaagtcg cggcgtaaag acggctctcc tcctgtcagg
     3481 cgcacttttt cggtgcccag actggcgaag gcgcgcgtaa cccggcgaat ttcatcgacg
     3541 gtaagaaagc ctttattggt gacgccgctc ggtttgtagc catccggcag gcagtaggtg
     3601 caacgaaagt tacacacatc ggtaatcgac aggcgcaagt agtaaaactt acgcgcaaat
     3661 acatcagtca gttgtgaagc catgtacacc tttccagata cgggaggcga agtcatttct
     3721 tccttcgccc tggtggcaat ctttacgagc attgtcacgg ccaaagcacc gtatcagttg
     3781 acccaggtgc agaggctaga gtgtttagtg gttaagccga tactagcgtg taaatgagat
     3841 ttttaccatc cacactttcg ctatgtaatc atatatatag cgtcatgatc gtgctatttc
     3901 gctacagaat aagggaaaat ggcggtttta ctttgatata catcatcagg gaggcatagc
     3961 gtgcatcgtc ctttagagat agaagaaaat agtttgtatc ctcaatattg gcaggttaat
     4021 tgctgtttcc ccgcaatttg cgctactgta gcgcggataa tctgactcca ggaaactata
     4081 tgcgcaatcg tacgctggct gatcttgatc gtgtcgttgc tctcggcgga gggcatggac
     4141 tgggacgcgt tctctcatca ctttcgtctt tgggttctcg tttaacgggt atcgtcacca
     4201 ccacggataa tggtggctcg acggggcgta ttcgccgttc agaaggcggc attgcttggg
     4261 gcgatatgcg caactgcctc aaccagctga taacggaacc gaacgtcgcc tccgcgatgt
     4321 ttgaataccg ttttggcggc aatggcgaac tttccggtca taatctcgga aacttgatgt
     4381 taaaggcgct ggatcacctt agcgtgcggc ctctggaagc catcaattta attcgtaatc
     4441 tgctgaaagt ggatacgcat ttgattccaa tgtcagagca tcctgttgat ctgatggcga
     4501 ttgacgatca ggggcatgaa gtttacggcg aggtcaatat cgaccagtta actacgccga
     4561 ttcaagagtt attgttaacg cctaatgtac ccgcaacgcg tgaggcggtt cacgctatca
     4621 atgaagcgga tctcatcatt attgggcctg gcagttttta taccagcctg atgccaattc
     4681 tgctgctgaa ggaaatcgcc caggcattac gccgcacgcc agcgccgatg gtttatatcg
     4741 gcaatctggg gcgtgagttg agtttacctg cggctaattt gaagctggaa agcaagctgg
     4801 caattatgga gcagtatgtt ggtaaaaagg tcattgatgc ggtcatcgtc gggccaaaag
     4861 tggatgtctc ggcggtgaaa gagcggattg tgatccagga ggtactggag gccagcgata
     4921 ttccgtatcg tcatgaccgc cagttgttac ataacgcgct ggaaaaggcg ttacaggctt
     4981 taggttaacg gaaatccgaa ggaaaattcc ggcttcctat tgaagacaaa gtgcgcgttg
     5041 tttatgccgg atgcggcgtg aacgccttat ccggcctaca aaccgcgcaa attcaatata
     5101 ttgcggagaa aatgtaggcc tgataagcgt agcgcatcag actgttttcc gtttgtcatc
     5161 agtcttcttc gctatcctgt tacgatgcgg caataaacaa ctcacgcaac tgatgcaact
     5221 ggtcacgaat ttgtgccgct tcttcgaact ccagattctg cgcgtgttgc atcatcagcc
     5281 cttccagttc atggattttc tgctgcaacg ctttcggcaa catatccatc ggcacattat
     5341 ccggctcaac aatcgggcgc gattttcctc tgcccttcgc tttggttttg gcaatgttct
     5401 gccccagcgc caggatatcg accactttct tgttcaagcc ttgcggcgta atgccgtgtt
     5461 cctcgttgta cttctgctgt ttctcacggc gacgttcggt ttcgccaatc gctttcgcca
     5521 ttgatggagt gctcctatcg ccgtagagaa tcgctttacc gttaacgtta cgtgccgcac
     5581 gaccaatggt ctggatcaac gaacgttcgg aacgcaggaa gccttctttg tcagcgtcga
     5641 ggatcgccac cagcgacact tccggcatat ccagaccttc acgcagtaag ttgatcccta
     5701 ccaatacgtc gaactcaccc agacgcaagt cgcggataat ctccatacgc tcgacggtgt
     5761 cgatatctga gtgaagataa cgcacgcgct caccgtgttc ttcgagatat tcggtaagat
     5821 cttccgccat tcgcttggtc agtgtggtga ccagcacgcg ttcgttaatt gccgctcgct
     5881 gacgaatctc cgaaagaaga tcatcaacct gtgtcgccac cggccgcact tcgataatcg
     5941 ggtcaagcaa tccggttgga cgcaccacct gatccaccac atcgccgccg gatttttcca
     6001 gctcgtaatt acccggcgtc gccgaaacat agatggtttg cggcgctaat gcttcgaact
     6061 cttcaaactt aagcggacgg ttatccagcg ctgatggcag gcggaagccg tactccacca
     6121 gtgtctcttt acgcgcccgg tcaccgcgat acatgccgcc aatttgtgga atggtgacgt
     6181 gagattcatc gacgaccagc agcccatcgg caggcaggta atcaaacagc gtcggcggtg
     6241 gctcacccgg tccacgaccg gagaggaagc gcgagtagtt ttcaatcccc gaacagtagc
     6301 ccagctcgtc catcatctcc agatcaaact gggtacgctg ggtcagccgc tgctcttcca
     6361 gcagtttgtt gttttccaac agcactttgc gtctggcggc cagttcttct ttgatctcct
     6421 ccatcgcctg tacgatgcgc tcgcgcggtg tgacgtagtg cgttttcggg tagatggtaa
     6481 aacgtggaat agtggaaaca atctgcccgg tcagcgggtc aaataacgac aatcgttcca
     6541 cttcctcgtc aaacagttcc acgcgaagtg caatgtcatc cgattctgcc gggaagatat
     6601 ctatcacctc gccacgaacg cggaaagtac cacgctggaa tgcttgatca ttacgagcgt
     6661 attgcagctc cgccagtcgg cgcagaatcg cgcgctgatc gataatcata ccgaccgtga
     6721 gatggagcat catcttgaga tataaatcag gatcgcccag accataaatc gcggaaacag
     6781 acgccactac aatcacatca cgccgctcca gcatcgcttt ggtggcggac aaacgcatct
     6841 gctcaatatg ttcgttaacc gaggcatctt tctcaatgaa ggtgtcggaa ctcggtacat
     6901 aggcttccgg ctgatagtag tcgtagtagg agacgaaata ctccaccgcg ttttccggga
     6961 agaactcttt catttcgcca tacagctggg ccgccagcgt tttgttgggc gcaagtacca
     7021 tggttgggcg ctgaaggtca gcaatgacat tggcaatggt gaaggttttc ccggagccag
     7081 tcacgccaag taacgtctgg tgcgccaggc catcttccag cccctcttcg agacgtcgaa
     7141 tcgcctctgg ctgatcgcca gaaggtttaa aagcggaatt cagtttgaac ggtttactca
     7201 tgagtcgcta cctgaaggag ttgggcgggc aggtatgtaa ttttactcgt cgtacttaat
     7261 tatgccaaca aattatactg gataaaaaaa cagttcatca ccataatatt tctgatacag
     7321 cgtaaactcc gctgtcaatc atgagcaaaa tttactctgt ggcgagataa aactccagcc
     7381 ttaccgggtt atccccaaag caacggcttt tttaacattt gtcaagatga gtgatgacag
     7441 ttttatggca agagatgcct gttcagtgac tgccattgca tttatctaac cagttaaaaa
     7501 ataaaagata tttctttgag ccgtctttaa cgccaattcg cgtgcaagcc gcgtattctc
     7561 tcgcttgcct cgtgttttct aactctaata cacatggtta tccacaggaa tagtggataa
     7621 ctgtctccag cccctatccc ccgccgcctg ggcaattccc acattcgccg catgaggcgg
     7681 aagtaaaatt tttttcgtga ttattttaga atttaattgg ttaaattgca gtcaatcgaa
     7741 gacgcgatct cgctcgcaat ttaaccaaat acagaatggc tacaacaagg caaggtttat
     7801 gtactttccg gttgccgcat tttctggatt ttctgcaagc caggggatct ctcccagcag
     7861 cggcgcggga atcatgcggg tgagcgtggt catatattca gcgtgacgtt ttcccggagg
     7921 cgtaacatcg ttcgccaccc aacccgccag agtcagtccg gcgtgttgta ttgcctgcgc
     7981 agtcaacatc gcgtgattaa tacagccgag tttcacacca actaccagta tcaccggcag
     8041 ttgttcctgt gttacccaat ctgcaaaagt gaaagtgtca gaaagcggcg taaaccagcc
     8101 gccagcacct tccactaaca cccagtcagc ctgttgttca agcgcgcgta atccggcgct
     8161 cattaccaat gattctatcg gcctgccctc ttgggcgctg atgatgtgcg gcgaggtgga
     8221 ttctgcgaag gtgtaaggat ttaccgttgc gtaatccagc tgcaggctgc tgttgtgctg
     8281 taacgccagc gcgtcactat tacgtaagcc ttccggggtc ttttcgctgc cagaggcgac
     8341 cggtttataa cctgccgtcc gataacctac tgccttcgcg gcttgtaaaa gtgcacaact
     8401 ggcgacggtt ttccccactt cggtatccgt tccggtgaca aaataacgtt tactcacgag
     8461 caatcactcc caaaaaaaga tgatacgtca gaggatatcg cccctgttgt tgcggccagg
     8521 ccagttgcaa tcgctgcaac tgcgaacgcg ttaatattcg cgggtcgcgc ccttcatgaa
     8581 gatgcgtagc accgatgcct ttcagcgaac gcatggcact gagcgcatca tcaaaccata
     8641 gtgtgatgag ctgaatatga tgttgataac gcacgccgtt cagcgactct tcgatttcat
     8701 ctggcggtaa aaagcgatta gcatgcggac gctcgtccac cgcctgccgc gcctgatgca
     8761 gttcgggtaa cgatccctgc accagcgtgg taaacgcgac cacgcctccg gggcgcacca
     8821 cccgatacag ctcgcggagt gccgtggata aattaccgca ccactgcacc gcgagattgc
     8881 tccatgcaag atcgaacgtc gcagtcgcta acggcaggga ttcgatatct cctgccagat
     8941 aatgatctgc ggcatcttta tggcgggctt gcgcaagcat tggcggcgag agatctaacg
     9001 ccgtcacctg cgcgtgacgt tcccgccagt ggcggctcat ccagccaggt ccacaacccg
     9061 cgtccagtac gtgggtgtat ttacgctgtg gaagcattgc cagtaaggcg tcagcactct
     9121 ggcgctgtag atctgcatgt tgctcatagt gtgcggctgc ccgaccaaat gccgctgcaa
     9181 tggcttgttt attaaccgtt gccatgcagc acctccagca gacggtcgat atcctgcatt
     9241 tcatgcgcag cggttagcgt taagcgcagt cgcgcagtac cagcgggtac ggttggcggg
     9301 cgaatcgccg tgacccagca gccttgctgg cgcagttttt ctgccagttg taacgcacgg
     9361 ctgttatcac cgacgatcaa tggctggatg gcgctgcatg aatcagccag cgtaaacggc
     9421 aaatcctgta ctccggcacg aaaacgcgca atgagtgaca ccagtttttc gcgccgtgca
     9481 tccccctcat cacggcgaat gaccgccagc gacgcacgta atgcctgcgc ctgagcgggc
     9541 ggcatactgg tgctgtagat aaggtggcgg gcgaattgca gcagataatc cgccaccgta
     9601 cttgagcaaa gcaccgcagc cccactgacg ccaaatcctt tgccaaaagt cactaccagc
     9661 aattctggtt ttaccttttg cagccagcag ctgccgcgcc cctgctcccc gataacgccc
     9721 gtgccgtggg catcatcgac catcaaccag ccattgtgct gttgcgttac ctgctggatt
     9781 tccgccagtg gcgcgctatc gccgtccatg ctgaacacgc cttctgtcac caccagttgc
     9841 tgccccggac agggggaagc aagcagtcgc gccagatgag cgacatcgtt atgagtaaaa
     9901 cggcgaagct gcgacgggct taaactggca gcctccagca atgaggcatg gctaagccgg
     9961 tcggcaacaa tacggtcctc tttcgccatc atcgcggcaa tgactgcctg attcgcggca
    10021 aaaccagaga taaacagcag cgcccgcgaa tagccaagcc actcggccag ttcttcttcc
    10081 agcgcctgat gcaccacgct ataaccgctg acgtgaccgg agccgccgct accgatgcca
    10141 aattgctccg ccccctgctt ccaggcacgg ataatttgcg gatgatggct taaacctaaa
    10201 taatcgttac tggaaaagtt cagatactgg cgatcatccg ccaccagcca gcgtccggct
    10261 ccttgcgcca ccggataacg gcgacgcagg gcatcggcag cacgccgcgc atcgagcgcc
    10321 gcgttgattt tctcctgcca gctcataatg ctgccgcgtt gtaatattcg tcggtgtccg
    10381 gggtcatcag cgcctgtcca agacgttgct gttgttcgtt atcccctgcc agcacggcag
    10441 tttgctgcgg atttagcccc agtttgcgga acagttgcag gtctttatct tcttccggat
    10501 tcggcgtggt cagcagtttg caaccgtaga aaatcgagtt tgcgcctgcc ataaagcaca
    10561 tcgcctgagt ctgttcgttc atctgctcgc gtccggcaga aaggcgcacg taagaggtcg
    10621 gcatcatgat ccgcgctacg gcaatggtgc gaataaaatc aaaggcatca acgtcatcgt
    10681 tatcggcaag cggcgtgcct ttcaccttca ccagcatgtt gattggtacg ctttccggcg
    10741 gcgtcggcag gtttgccagt tgcagcaata atccggcgcg atcttttacc gtttcgccta
    10801 agcccacaat gccacctgag cagactttga tcccggcatc gcgcactttt tccagcgtat
    10861 cgaggcgttc ctgataagtg cgtgtggtga tgatattgcc gtaaaactcc ggcgaggtgt
    10921 ccaggttgtg gttgtagtaa tccagcccgg cgttcgcgag gcgctgcgcc tgagattcac
    10981 tcaacgtgcc cagcgtcata cacgcctcca gccccatcgc ttttaccccc tgcaccattt
    11041 gttccaggta cggcatatcg cgttcgtggg gattcttcca tgccgcgccc atacagaagc
    11101 gcgtcgatcc tgccgctttc gctttgcgcg ccgactccag cacctgttca acttccatca
    11161 accgctcggc ttccagcccg gttttgtagc gcgagctttg cgggcagtat ttgcaatctt
    11221 ccggacaagc tccggtctta atcgacagca acgtgctgac ctgcacctga cgaggttcga
    11281 aatgctggcg atgcacctgc tgcgcttcaa acagcagatc cagcaacggt ttttcaaata
    11341 attctgtgac ttgcgacaat gtccagcgtg ggcggtgagc catggggctt ctccaaaacg
    11401 tgttttttgt tgttaattcg gtgtaggctt gtaaacctaa atcttttcaa tttggtttac
    11461 aagtcgatta tgacaacgga cgatcttgcc tttgaccaac gccatatctg gcacccatac
    11521 acatccatga cctcccctct gccggtttat ccggtggcga gcgccgaagg ttgcgagcta
    11581 attttgtctg acggcagacg cctggttgac ggtatgtcgt cctggtgggc ggcgatccac
    11641 ggctacaatc acccgcagct taatgcggcg atgaagacgc aaattgatgc catgtcgcat
    11701 gtgatgtttg gcggtatcac ccatgcgccc gccatcgagc tatgccgcaa actggtggca
    11761 atgacgccgc aaccgctgga gtgcgttttt ctcgcggact ccggttccgt agcggtggaa
    11821 gtggcgatga aaatggcgtt gcagtactgg caagccaaag gcgaagcgcg ccagcgtttt
    11881 ctgaccttcc gcaatggtta tcatggcgat acctttggcg cgatgtcggt gtg
//
LOCUS       DAZXUK010000114        11818 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZXUK010000114 DAZXUK010000000
VERSION     DAZXUK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11818
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.114"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..95)
                     /locus_tag="ACH3L8_002408"
     CDS             complement(<1..95)
                     /locus_tag="ACH3L8_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085487.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGD"
     gene            complement(233..2074)
                     /gene="rpoD"
                     /locus_tag="ACH3L8_002409"
     CDS             complement(233..2074)
                     /gene="rpoD"
                     /locus_tag="ACH3L8_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFT4085488.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(2269..4014)
                     /gene="dnaG"
                     /locus_tag="ACH3L8_002410"
     CDS             complement(2269..4014)
                     /gene="dnaG"
                     /locus_tag="ACH3L8_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFT4085489.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(4125..4340)
                     /gene="rpsU"
                     /locus_tag="ACH3L8_002411"
     CDS             complement(4125..4340)
                     /gene="rpsU"
                     /locus_tag="ACH3L8_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFT4085490.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4578..5591
                     /gene="tsaD"
                     /locus_tag="ACH3L8_002412"
     CDS             4578..5591
                     /gene="tsaD"
                     /locus_tag="ACH3L8_002412"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFT4085491.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(5634..7097)
                     /gene="ttdT"
                     /locus_tag="ACH3L8_002413"
     CDS             complement(5634..7097)
                     /gene="ttdT"
                     /locus_tag="ACH3L8_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFT4085492.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(7145..7750)
                     /gene="ttdB"
                     /locus_tag="ACH3L8_002414"
     CDS             complement(7145..7750)
                     /gene="ttdB"
                     /locus_tag="ACH3L8_002414"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFT4085493.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(7747..8590)
                     /gene="ttdA"
                     /locus_tag="ACH3L8_002415"
                     /pseudo
     CDS             complement(7747..8590)
                     /gene="ttdA"
                     /locus_tag="ACH3L8_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            8797..9798
                     /locus_tag="ACH3L8_002416"
     CDS             8797..9798
                     /locus_tag="ACH3L8_002416"
                     /inference="COORDINATES: protein motif:HMM:NF007315.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFT4085494.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     ELLYRSMKFVSARNFWRHGASNGWASPMKAIRSCRSTRNHSGSFSRFLLNFVQIFVS"
     gene            complement(9741..10358)
                     /gene="plsY"
                     /locus_tag="ACH3L8_002417"
     CDS             complement(9741..10358)
                     /gene="plsY"
                     /locus_tag="ACH3L8_002417"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFT4085495.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            10463..10831
                     /gene="folB"
                     /locus_tag="ACH3L8_002418"
     CDS             10463..10831
                     /gene="folB"
                     /locus_tag="ACH3L8_002418"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFT4085496.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            10922..11743
                     /gene="bacA"
                     /locus_tag="ACH3L8_002419"
     CDS             10922..11743
                     /gene="bacA"
                     /locus_tag="ACH3L8_002419"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFT4085497.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
BASE COUNT         2704 a         3017 c         3202 g         2895 t
ORIGIN      
        1 taatccccca gagaagtcag tgggttacgc agagaatcgt aacgggatac cagatcaaga
       61 gcctgtccaa tgtgcataaa aaaatccgga aacaagtgag cgtttccgga ttcttacaca
      121 gccactggat cggtcaactg atccttaact gatcggcatt acaccgaaaa ggtggcgttt
      181 gtgcagagag actagtgccg ggtgcggcgt aacgcctgat ccggcctacc gattaatcgt
      241 ccaggaagct acgcaacact tcagaacggc tcgggtgacg cagtttgcgc agcgccttcg
      301 cttcgatctg acggatacgt tcgcgggtaa cgtcgaactg tttacccact tcttccagcg
      361 tgtggtcggt gttcatatcg ataccgaaac gcatacgcag aacttttgct tcacgcgcgg
      421 tcaggccagc cagcacgtcg tgcgttgccg cacgcaggct ttcggtggtc gcagaatcca
      481 gcggcagctc gagggtggta tcctcgatga aatcccccag atgcgaatct tcatcatcac
      541 cgatcggcgt ttccatggag attggctctt tggcgatctt cagcactttg cggatcttgt
      601 cttccggcat cagcatacgt tcagccagtt cttccggcgt cggttcacgg cccatctctt
      661 gcagcatctg gcgagaaata cggttgagtt tgttgatagt ctcaatcata tgcaccggaa
      721 tacggatggt gcgcgcctga tccgcgatag aacgggtgat cgcctgacgg atccaccagg
      781 ttgcgtaggt ggagaacttg taaccacggc ggtattcgaa tttatcaacc gctttcatca
      841 ggccgatgtt accttcctga atcaggtcaa ggaactgcaa gccacggttg gtgtatttct
      901 tggcgataga aataaccaga cgtaagttcg cttcaaccat ctctttcttc gcacggcggg
      961 ctttcgcttc accgatggac atacgacggt tgatatcttt aacctgctcg atggtcaggc
     1021 cggtttcttc ttcaatctgc tgcagttttt gcagggcgcg gtgcacttct tcagagacat
     1081 cgtgcagttt ttccgaccac ggcttgttca tcgcaattgc cgcgttgaac caggtatcgc
     1141 tggtttcgtt gccggtaaac agggtaatga agtttttctt cggcattttg cactgctcaa
     1201 cgcagagctt catgatcaga cgttcttgcg tacgaacgcg gtccatcatg acgcgcatgc
     1261 tgttgaccag gtagtcaaac tgcttcggca ccaggcggaa ctgtttgaat acttcagaca
     1321 gtttcaggat ctcttcctga gcggcagcgt gactgcgacc tttcgctttg atggtgtcac
     1381 gcgttacaac gtactgagcg cgtagttccg caaatttttc gcgagccagt tccgggtcga
     1441 tgctgttgtc atcatcggcg ctgtcgtcat cgccatcttc ttcgtcttca tcttcgtcat
     1501 cgtccagatc ttcctgggaa agctcagaac cgacgtgagt ggcggtaggt gccagatctt
     1561 cttctgcgtt cgggtcaaca aagccggtga tcagatcgga cagacgcgct tcttctgctt
     1621 caacacgatc gtactgttcc agcagatagg tgatcgcttc cggatattca gcaacggagc
     1681 attgaacctg gttgatcccg tcttcaatac gcttagcgat gtcaatttcg ccttcgcggg
     1741 tcaacagttc aacggtgccc atttcacgca tgtacatgcg taccgggtca gtcgtgcgcc
     1801 cgatttcaga ttccacgctg gaaagcacct gcgcggcggc ttcggcagca tcttcgtccg
     1861 cggtgttttc agccagcatc agatcatcgg catccggtgc ttcttccatc acctgaatgc
     1921 ccatgtcgtt gatcatttgg atgatgtctt cgatctgatc tgaatcgacg atatcttccg
     1981 gcagatggtc attgacctcg gcataggtca gatagccttg ctccttacca cgggtgacaa
     2041 gaagtttcag ctgtgactgc gggttttgct ccataagacg gtatccacac ttatttcatg
     2101 aggttggtgt tgggcgattg acccgatgtc cttcagccgt taacgctgaa atccaacggt
     2161 tgtttaccgc caacgataat tacgagggcg tacttatata tttgccgctg cctctcagtg
     2221 cggctgtcgg gggcttcccg atcgctcttc ggcacttaag ccgttaaatc actttttcgc
     2281 cagctcctgg tttaatgtcc agagctccag gcgttcttcg ttgcttaaac catgcgtgcg
     2341 ctcacgagcg attaactctt cctggcgcag ttcaagcagc gaatcaaaca tatggttgag
     2401 tgagtcggtg aaggtttgct cagcaatatt cttatctgct atatcgtccc acatcgacag
     2461 tttttcaagg gtggcagcat tatttgtacc acgatagtgc tctaaaagtt gcccggtggt
     2521 cagacctggc tgggagagac aagtgttgac cagttctctg aataagccaa gtccagggag
     2581 cttattttca tccagattct caagcggcgg gaccaacgtc gctaattctg gattttgcac
     2641 cagcaaccct ataagtatac gcatggtcgt gcgttttagc tgcggaacag ggcgagaaac
     2701 gccgctctct gccgcttttg gcattaatcg ttcaagctgg ctgtcatcaa gtatgcctaa
     2761 tttgttgcct aattcctgac gaagatatat tcgcagcgtt tcgcccggca cttgcgatat
     2821 caatggcagt gccagcgtac tcaaacgtgc gcgcccgtca ggggtactca gatcaacttg
     2881 cggcatcaga ctgttaaaca gaaatgcgga gagtggcatc gcctgctcca tccgcgcttc
     2941 aaacgcttct ttaccttctt ttcgtaccag cgtgtcaggg tcttcgccat ccggcaaaaa
     3001 cataaagcgt agctgacggc cgtctgtcat gtaaggcagc gccgtttcca gcgctcgcca
     3061 tgcggcatcg cggcctgcac ggtcgccgtc ataacagcaa atgacattgt tggtcgcgcg
     3121 gaacaacagt tgtatgtgat cggcggtggt tgacgtacct aacgacgcaa cggcgtaatt
     3181 aatgccgtat tgcgccagcg ccaccacgtc catatagcct tcgaccacaa gcagacgatt
     3241 gggttcagcg ttatcctgct gcgcttcata aagaccgtaa agctggcggc ctttatggaa
     3301 aatgtctgtt tccggcgagt tcaggtattt gggggtatcg ttgcccagca cgcgcccgcc
     3361 aaaaccaatc acccgaccgc gtttatcgcg aatggggaac atcacccgtt cgcggaaacg
     3421 atcgtaactg cgtccctgat cgttagtgac caacatcccc gcatcaatca atgactggcg
     3481 attttctgga ttgccgccaa accgcttcag gacgttgtcc cagccggggg gcgcaaaacc
     3541 aatcgcaaag cgagcgataa cctcgtggct taatccgcgt ttttccagat actggcgcgc
     3601 agacgtggca acaggttgtt gtaaagattg ttggtaaaac gtattcagac cgtccatcaa
     3661 ctgataaagc gtttgcctct gatggcgctc gatctggctg gggccgctgc ctgcttcaaa
     3721 tggcacttca agattgtgca ttgctgccag ctcttcgacc gtttcgacga actcgagctt
     3781 gtcgtagttc atcaggaagt cgatcgcgtt gccgtgcgcg ccacatccaa agcagtggta
     3841 aaactgtttc tcaccgttaa cggtgaatga cggggttttc tcgttgtgga atggacaaca
     3901 cgcgtggaaa ttcttgccct gctttttcag cttcacacgg gcatcgatca gatcgacgat
     3961 gtcagtgcgt gccagcagat cattaatgaa tacgcgtggg attcgtccag ccataggccc
     4021 cgatttttag caattcataa acgaaaataa gccgcgcatt cctttcggaa gcacggcctt
     4081 acaactacaa ctcggtctga tcggagagca acgctctcgg ggaattagta cagacgagtg
     4141 cggcgtgcgt tttcgcgagc cagtttcttc gcgtgacgtt tcactgcaga agctttagcg
     4201 cgcttacgtt cggtagtcgg tttttcatag aactcacgac gacgaacttc cgccagaaca
     4261 cctgcttttt cgcaggaacg cttgaagcga cgcagagcta cgtcgaacgg ctcgttttca
     4321 cgtactttaa ttaccggcat gtgcctctca cctttgatta attcggtttg ccgctggcat
     4381 caacgccagc ttatttcaaa atggtgcgga attttactgc aattgctgct gctttgtaaa
     4441 gcaccgcggc cttttttgaa agggactttt ataagggtga ggagtataca cgaggctttc
     4501 tccaggggcg aacaaagttt tacatcaacc cgcattggtc ctacactgcg cggtaataaa
     4561 gcgaggtaaa acaagtcatg cgtgtactgg gtattgaaac ttcctgcgat gaaaccggca
     4621 tcgccattta cgacgatgaa aaaggtttgt tagccaacca attgtatagt caggtgaaat
     4681 tgcacgctga ctacggcggc gtcgtgcctg aactggcctc ccgcgatcat gtgcgtaaaa
     4741 ccgtaccgtt gatccaggcg gcgctaaagg agtctggttt aacggcaaaa gacattgatg
     4801 ctgtggccta taccgcaggc cctggattag tcggcgcact gctggttggc gcaaccgtgg
     4861 ggcgttctct ggcgtttgcc tggaacgttc cggcaatccc ggtacaccat atggaagggc
     4921 atctgttagc gccgatgctg gaagataacc cgccggaatt tccgtttgtc gcgctgctgg
     4981 tgtccggcgg tcatacgcag ttaatcagcg tgactggcat tggtcagtac gagctgctcg
     5041 gcgagtctat cgatgatgcc gccggtgaag cgtttgataa aaccgcgaag ctgctggggc
     5101 tggattatcc tggcggaccg ttactgtcga aaatggcggc tcagggtact gccgggcgct
     5161 ttgttttccc gcgtccgatg accgaccgtc cggggctgga tttcagcttt tctggtctga
     5221 aaacctttgc ggcgaacacg attcgtgaca acggcaccga cgaccagacg cgtgctgata
     5281 tcgcccgcgc ctttgaagat gcggtggtcg atacgttgat gattaagtgt aagcgagcgt
     5341 tggatcagac gggctttaag cgactggtca tggcgggcgg cgtgagtgct aaccgcacgt
     5401 tacgggcgaa gctggctgaa atgatgaaaa aacgccgcgg cgaagtgttc tacgcgcgtc
     5461 cggaattttg tactgataac ggcgcgatga tcgcctatgc cggaatggtg cggtttaaag
     5521 caggcgcgac ggcggatctc ggcgttagcg tgcgtccgcg ctggccgctg gcggagttac
     5581 cggctgcgta aaactattgg gtgccggaga gcaatttccg gcaccgtcct cacttaaagc
     5641 aacaccacgg gcatccacag taagccggta atcaccagca acacgaggaa tatcagccca
     5701 aaaatcgccc ccagtcgcca gtaatccgcc gttggcagat aaccactacc gtagtaaatc
     5761 ggactgggtc cggtggcgta tggtgtaaga atgctcccca gaccaattgc cgcgccaacc
     5821 atcaggcaga acaccggcag cgggatttcc ggcatcgcca gcgcggcggc gatcatcatc
     5881 ggtgcgaggg cgcaggtata cgccgtggcg ctggcgaaaa agtagcgcag tagataaaac
     5941 accacaatca acgtcaccat caccatcgtc ggcgaataac cacttaagct gcctgctaac
     6001 agtttgccaa accagctaat aaaaccggta ttgttgagtc cggtcgcgag ggtgataagc
     6061 gaggccagcc agaagaaaac gttccatgcc gctttattac tgacaatgtc gtcccaactg
     6121 ataatgcgca gaagcagcat cagcgccacc acgctgtaac cgaccatcgc ggcatcgata
     6181 taatcaccgc cgaaaatcca cagcaccagc gcgcctacca tcagccccag catccgtttt
     6241 tcacgcgaac agagcgggcc cattgcctgc agttccgtct ctgcccagcg cggcacctga
     6301 tcaccagact tcagtaccgg cgggtacagc acgtaagcca gccagggaac cagcagaacc
     6361 agtaaaatgc tgagcggcaa catcccgagg aaccagtcgc cccaactcag cgtggcgtga
     6421 gatgcgcttt tcatcagtcc aattaacagc aagttaggtg ccatcgccgt caggaaaatg
     6481 gcgctggtca cgcagtcggc aacaatcccc atccacatga tgtacgagcc aatgctgcgc
     6541 gaactgctgt cgtttggttg tgattgatag agcggtggca ggttacggat gatgggatag
     6601 ataatacccg caccacgcgc cgagttggac ggtgttacag gtgccaggat cagctcggag
     6661 aacatcaccg catagccgag aaacagcgtg cgatgcccca tctttttcac cagaatcagc
     6721 gcaatgcggc gtccaagccc ggttttttca tagcctgtgc caaacataaa ggcggcgaaa
     6781 atgagccaga taaccgaatt agaaaaacca gaaactgccc aggagaggga tttggcggtg
     6841 aacttaaagc ctggctgggc gagctgttcg gggctgaaca gcagccaggg agagagtatg
     6901 gcgatgatgg agatacccac catcgccacc acggcacccg gcacgggttc gaggatcagt
     6961 ccaacgatca cgccagtaaa aacggcaaag tagagccagg tatggttctc cagccctgcg
     7021 ggaaccggaa gtagagcaat aatggcgatg accgccagcg gcgcaagata tcgccaccat
     7081 tcagtggaag gtttcatcgt gttatgcctg cgaaaaagga gaggggtgag cctctccggg
     7141 agggttattt gatgtaatgg acgtgctcgc agatctcttc cacgatggga tcgcggcgtt
     7201 cggcgaacag ctttttgttt tcggctatca ggttgttgcc gtgggtatca atagagacaa
     7261 tcagcgggcc gaactctttg acccggcaga cccacagtga ctccggcatc ccgagttctg
     7321 tccagtgcac ttcttcaatc tcttctacct gggttgccgc cagcaccgcg cagcctgctg
     7381 ggaaaatcac atgtaacgcc ttgaatttct ggcagccttc ttcggtcagc ggccccatac
     7441 cacctttgcc aaccaccagt tttacgccgg tctgctcaat aaattcacgt tcaaaacttt
     7501 ccatacgcat actggttgtc gggccgacgg agaccatctc ccatttgtcg ccgtttttgc
     7561 gcacgatggg gccagcgtgg aaaatcgctt tgccgttgag atcgtaaggg atcggacgtt
     7621 tcagttcgat caaacggcgg tgacaaacgt cgcggcaggt caccagcgta ccggtcaggt
     7681 agatcacatc gccgacgcga atatcttgca gatcttcagc tttgatcggg gttgtcagga
     7741 tctttttcat aacgcgctcc gggtgtgaga cagattttca aaggtgagat cggcatgaac
     7801 cagcagcgtg ccgcgacgat gcgcccatca ctgaactgtt gccggtcagc ccttgtggac
     7861 caatccccag acggttgagt ccttcttcca ggcgtagctc cagttctgcc gcttttggat
     7921 tgggatggcg gctgccaatc gggcgcaaaa tggctttacg cgagagtacg gcggcggttt
     7981 ccaccgaggt ggcgatgccc acgcccacca gtaccggtgg acaggcgttt acggcgaggg
     8041 tggagatatt ttcgaagacg aatttcacca cgccttcgta gccttctgac ggcattaaca
     8101 ctttcgagcg gccaggtagc gtgcagccgc cgcctgccat gtaaacttcg atttccgcat
     8161 cgtcattgtc ggggatgatg tcccaggtga cccacggtac gccgctaccg gtatttttgc
     8221 cggtgtttac ttcgtcaaaa atttctaccg cattgtgacg tagtggcgct ttgacggttg
     8281 cctcttccac ggcttgtttg agtatgcttt gcagctcgcc aagcagtggg aagcgggaac
     8341 cgactttaac gaagaacata atctccccgg tgtcctgaca ggcaggacga ttcaggtcaa
     8401 tcgctttttg catgttgtcg aacatcgtat ggtagataat tttccccatc gacgacgttt
     8461 cggcatcctt tagctgtttt agtttatcca ccacgtcatc aggcattcgg gtagaaatca
     8521 tggcggtaaa gttagcgaca atctctgtca acttattcac tgcctgttgc ttattacttt
     8581 cgctcatcat ggcaactcca gtttttgtat ttgtctggaa aaattcaccg ccacaaacta
     8641 acactaaaaa tgtggtttta tattttttgc caccgttaat atgtaattaa cagttcgtga
     8701 atcaacgcga aaataagaat gaatattgcg ccagagcagg gaatggcaat gttttgcgaa
     8761 atacactgcg cttatcacaa ctgcggataa ttaacgatgc tgaatagctg gcctttagcc
     8821 aaagacttgc aggtactggt agaaatcgtc cacagcggga gttttagtgc tgcagcggcg
     8881 acgcttgggc aaacgcctgc atttgtcacc aagcggatcc aaatccttga aaatacgctg
     8941 gcaacaacgc tgctgaaccg ctccgctcgc ggcgtggcgc tgactgaaag tggccaacgt
     9001 tgttacgaac atgcgctgga aatcctcacc caatatcagc gactggtcga tgacgtcacg
     9061 caaatcaaaa cgcgcccgga ggggatgatt cgtattggtt gcagttttgg ttttgggcgc
     9121 agccatattg cgccagccat taccgaactt atgcgcaatt atcctgagtt acaggtgcat
     9181 tttgaattgt tcgatcggca aattgattta gtgcaggata atattgatct ggatattcgt
     9241 attaatgacg aaattcctga ttattatatt gcgcatctgt taacgaaaaa taaaagaata
     9301 ttatgcgcag cacctgagta tctgcaaaaa tatccgcagc cacaatcatt acaggaatta
     9361 agtcgtcatg actgtctggt gaccaaagag cgcgatatga cccacggaat atgggagttg
     9421 gggaacggtc aggagaaaaa atcggtgaag gttagcgggc atctttcctc caatagcggc
     9481 gagatagtgt tgcaatgggc gctggagggc aaagggatta tgctgcgttc cgagtgggat
     9541 gtgttgccgt ttctggagag tggcaaactg gtgcaagtat tgccggagta tgcacaaagc
     9601 gccaatatct gggctgttta ccgggagctg ctctatcgca gcatgaaatt cgtgtctgcg
     9661 cggaatttct ggcggcatgg tgccagcaac ggctgggcaa gcccgatgaa ggctatcagg
     9721 tcatgtagat ccaccagaaa tcactcggga tccttttcgc gctttctttt gaatttcgtc
     9781 cagatttttg tctcctgacg acgccacaga cgctggatgt tgtcatgatg acgcagcagg
     9841 atcaggcaag agagcatcga aaccgggaag gtgaattgtg gcttaaacca ccagacataa
     9901 aacggagcaa tcagtgcact gacaatcgct cccagcgacg agtatccgct caatagcacg
     9961 gtcagtaacc aggttcccgc cattactccg gtgagatccc agctaattgg tgcgattgct
    10021 ccaaaagcgg tagcaacgcc ttttcctcct ttaaaaccga agaaaacggg ccagatgtgt
    10081 ccaagacagg cggcgatggc aattaagcct agccagaagg ggctgacacc taattcatac
    10141 gcgccccaga caggcaacat tcctttcaga acgtcgaaaa tcagtactgc tacggctgct
    10201 cccttgccac cgatacgtaa cacattggtt gcgcctggat tgccggagcc gctggttcgc
    10261 ggatcgggca gcccacacaa gcggcaaacc agaatggcac tggaaatgga gccgcagagg
    10321 tacgcgatga ggatcattcc aggcgcgatt gcactcataa gctgttccgt tttgaaaatt
    10381 cgtgttcaac gatgaatctg tggataatac gcacatttcg ccggaagtgg tatccggtta
    10441 gccaaaaagc aggcaggacg tgatggatat tgtatttata gagcaacttt cggtaatcac
    10501 cactattggt gtttacgact gggaacagac catcgaacag aagttagtgt tcgatatcga
    10561 aatggcatgg gataaccgta aagcggcgaa aagtgatgat gtggcggatt gcctcagtta
    10621 cgctgacatt gcagaaacgg tggtcagcca cgtcgagggg gcgcgttttg cgctggtgga
    10681 acgcgtggct gaagaggtgg cggagctgct gttagcacgc ttcaactcgc cgtgggtgcg
    10741 tatcaaactc agcaagccag gcgcagtggc gcgggcggcg aatgttggcg taatcattga
    10801 gcgtggcaat aatctgaaag aaaataatta attttacagc tgttaaacca aacggttata
    10861 acctggtcat actgcagtag ttcggacaag cggtacattt taataattta ggggtttatt
    10921 gatgagcgat atgcactcgc tgctgatagc ggcaatattg ggtgtggtcg aaggattgac
    10981 agaatttctg ccggtatcca gcacgggcca tatgattatt gtcggtcact tgttggggtt
    11041 tgagggcgac acggcgaaaa cctttgaagt tgtgatccag ttaggatcaa ttctggcggt
    11101 agtagtgatg ttctggcggc gtctgtttgg cctgattggc atccactttg gccgcccgtt
    11161 gcagcacgaa ggtgaaagca aaggtcgttt aacgctgatc cacattttgc tggggatgat
    11221 tccggcggtg gtactggggc tattattcca cgacacgatt aagtcattgt ttaacccgat
    11281 aaatgtgatg tatgcgctgg tcgttggcgg tttgttgctg attgccgccg aatgcctgaa
    11341 gccgaaagag ccgcgtgcgc cgggtcttga tgatatgacc tatcgtcagg cgtttatgat
    11401 tggctgtttc cagtgtctgg cgctgtggcc gggtttctcc cgttccgggg cgaccatttc
    11461 aggtgggatg ctgatgggcg tgagccgtta cgctgcttcc gagttctcgt tcctgctggc
    11521 ggtaccgatg atgatgggcg caacggcgct cgatctctac aaaagctggg gcttcctgac
    11581 aaccggcgat atcccgatgt ttgccgttgg gtttatcacc gcttttgtgg tggcgctgat
    11641 agcgattaaa accttcctgc aattgattaa gcgcatttcg tttatcccgt tcgccattta
    11701 tcgctttatt gtggcggctg cggtgtatgt cgtgttcttt tgattgagaa aaggttcggg
    11761 agcaggccgg gttttctccc tcgctctgta gggagagggc cggggtgggg gtgattgt
//
LOCUS       DAZXUK010000115        11650 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZXUK010000115 DAZXUK010000000
VERSION     DAZXUK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11650
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.115"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..655
                     /locus_tag="ACH3L8_002420"
     CDS             <1..655
                     /locus_tag="ACH3L8_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family substrate-binding domain-containing
                     protein"
                     /protein_id="HFT4085498.1"
                     /translation="GEAGELRIGFTSSAPFIRAVSDTLSLFRRDYPDVHLQTREMNTR
                     EQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPLMAMIPYDHPLANNPSVTLAELAE
                     LAELAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGL
                     GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            790..2010
                     /gene="mlc"
                     /locus_tag="ACH3L8_002421"
                     /gene_synonym="dgsA"
     CDS             790..2010
                     /gene="mlc"
                     /locus_tag="ACH3L8_002421"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFT4085499.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            2135..2830
                     /gene="bioD"
                     /locus_tag="ACH3L8_002422"
     CDS             2135..2830
                     /gene="bioD"
                     /locus_tag="ACH3L8_002422"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFT4085500.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(2783..4075)
                     /gene="clcB"
                     /locus_tag="ACH3L8_002423"
     CDS             complement(2783..4075)
                     /gene="clcB"
                     /locus_tag="ACH3L8_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFT4085501.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISQTLHRDSIYRQHTAQHS"
     gene            complement(4234..4848)
                     /gene="dmsD"
                     /locus_tag="ACH3L8_002424"
     CDS             complement(4234..4848)
                     /gene="dmsD"
                     /locus_tag="ACH3L8_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFT4085502.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(4891..5745)
                     /gene="ynfH"
                     /locus_tag="ACH3L8_002425"
     CDS             complement(4891..5745)
                     /gene="ynfH"
                     /locus_tag="ACH3L8_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFT4085503.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(5747..6364)
                     /gene="ynfG"
                     /locus_tag="ACH3L8_002426"
     CDS             complement(5747..6364)
                     /gene="ynfG"
                     /locus_tag="ACH3L8_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFT4085504.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(6375..8413)
                     /locus_tag="ACH3L8_002427"
                     /pseudo
     CDS             complement(6375..8413)
                     /locus_tag="ACH3L8_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(8474..9508)
                     /locus_tag="ACH3L8_002428"
                     /pseudo
     CDS             complement(8474..9508)
                     /locus_tag="ACH3L8_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            9566..11155
                     /locus_tag="ACH3L8_002429"
     CDS             9566..11155
                     /locus_tag="ACH3L8_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFT4085505.1"
                     /translation="MTDINHDNTAYPSSYFSGSPYLYGQVYTPSPDTRLTQINLLGVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNHTDYSTIGDSTSILKNEQGDRPVVFTQE
                     RQPEVTICSFSELVSSLLAEKVQTLKSSSYKDLSSSLNTVSRFLPEDMDLMSRSEWLA
                     VRDAMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDVDSKRRAFT
                     DEEMERLLVRVESEYQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGACGLDLKSFLEWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDATDVSLHSLRHWLATRMKERGANLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLAEVLKTALL"
BASE COUNT         2873 a         2968 c         3021 g         2788 t
ORIGIN      
        1 gggtgaagcg ggggagttgc gtattggttt tacttcgtcc gctcctttta ttcgggcggt
       61 gtccgatacg ttatcgctgt ttcgccgtga ttatcctgat gtccatttac aaacccgcga
      121 aatgaacact cgcgagcaaa tcgctccgct cattgaagga acgctggata tgggattgct
      181 gcgtaacaca gcgttaccgg agacgcttga acacgcagtc atcgtccatg aaccgcttat
      241 ggcgatgatc ccgtacgatc atcccctggc aaataatccg agtgtaacac tggctgaact
      301 ggctgaactg gctgaactgg ctgaactggc gaaagaaccc tttgtctttt ttgatccgca
      361 cgtcgggact gggctgtatg acgatattct cggactgatg cgacgttacc atttgacgcc
      421 cgtcatcact caggaggtgg gcgaggcaat gaccatcatc ggtctggttt ccgccggtct
      481 gggtgtttca attttgcctg cgtcatttaa acgtgttcag ctcaacgaaa tgcgctgggt
      541 gccgattgct gaagaggatg cggtttctga aatgtggttg gtctggccga aacatcatga
      601 acaaagtcca gctgcgcgta actttcgtat tcatctgctg aatgctctca ggtgagggaa
      661 atttcagcga aaaagcccga aaaatgtgct gttaatcaca tgcctaagta aaaatttgac
      721 gacacgtatt gaagtgcttc accatagcct acagattatt tcggagcgcg aaaatatagg
      781 gagtatgcgg tggttgctga aaaccagcct gggcacattg atcaaataaa gcagaccaac
      841 gcgggcgcgg tttatcgcct gattgatcag cttggtccag tctcgcgtat cgatctttcc
      901 cgtctggcgc agctggctcc tgccagtatc actaaaattg tccgtgagat gctcgaagca
      961 cacctggtgc gagagctgga aatcaaagaa gcggggaacc gtggccgtcc ggcggtgggg
     1021 ctggtggttg aaactgaagc ctggcactat ctttctctgc gcattagtcg cggggagatt
     1081 ttccttgctc tgcgcgatct gagcagcaaa ctggtggtgg aagagtcgca ggaactggcg
     1141 ttaaaagatg acttgccatt gctggatcgt atcatttccc atatcgatca gttttttatc
     1201 cgccaccaga aaaaacttga gcgtctaact tcgattgcca taaccctgcc gggaattatt
     1261 gatacggaaa atggtattgt acatcgcatg ccgttctacg aggatgtaaa agagatgccg
     1321 ctcggcgagg cgctggagca gcataccggc gtaccggttt atattcagca tgatatcagc
     1381 gcatggacga tggcagaggc cttgtttggt gcctcacgcg gggcgcgcga tgtgattcag
     1441 gtggttatcg atcacaacgt gggggtgggc gtcattaccg atggtcatct gctacacgca
     1501 ggcagcagta gtctcgtgga aataggccac acacaggtcg acccatatgg taaacgctgt
     1561 tattgcggga accacggctg cctcgaaacc atcgccagtg tggacagtat tcttgagctg
     1621 gcacagctgc gtctcaatca atccatgagc tcgatgttac atgggcaacc gttaaccgtg
     1681 gactcattgt gtcaggcggc attgcgcggc gatctactgg caaaagacat cattatcggg
     1741 gtgggcgcgc atgtagggcg cattcttgcc atcatggtga atttatttaa cccacaaaaa
     1801 atactgattg gctcaccgtt aagtaaagcg gcagatatcc tcttcccggt catctcagac
     1861 agcatccgtc agcaggccct tcctgcgtat agtcagcaca ttagcgttga gagtactcag
     1921 ttttctaacc agggcacgat ggcaggcgct gcactggtaa aagacgcgat gtataacggt
     1981 tctttgttga ttcgtctgtt gcagggttaa cattttttaa ctgttctacc aaaatttgcg
     2041 ctatctcaat ttgggccagg aaagcataac ttagactttc aaggttaatt attttcctag
     2101 tttatatttg tgaagcataa cggtggagtt agtgatgctg aagcgtttct ttattaccgg
     2161 tacagacact tctgtaggga aaacggtggt ttcccgcgca ttgctacaag cgttagcctc
     2221 ccagggaaaa agggttgcgg gatataaacc cgtagcgaag gggagcaaag agacacccga
     2281 agggctgcgt aataaagatg ccttggtgtt gcagagtgtt tcaaccatcg aactgcctta
     2341 tgaagcagtt aatcctatcg cgttaagcga agaagaaagt agcgtggcgc acagttgccc
     2401 aatcaattac accctcattt caaacggcct ggcaaacctg accgataaag tcgatcatgt
     2461 cgtggtagaa gggactggcg gctggcgcag tctgatgaat gatttgcgtc cactctctga
     2521 atgggtagtg caggaacaac tgccggtgtt gatggttgtc ggtattcagg aaggttgcat
     2581 taaccatgca ctgctaacag ctcaggcgat cgccaacgac gggctgccgc tcattggctg
     2641 ggtggctaac cgaatcaacc caggactggc gcattatgcg gaaatcattg atgtgctggg
     2701 taaaaaactt ccggcaccgc tcattggtga actgccttat ctgccgcgcg ccgaacagcg
     2761 tgaactgggg caatacatcc gcttagctat gctgcgcagt gtgctggcgg tagatagagt
     2821 cacggtgtaa cgtctgcgaa attaccgacg caattacgca ggcaatcaat aaaccgggga
     2881 gtagctgata ctccccggtc atttcacata tcatcaacgt cgacataatc ggcgcgtgcg
     2941 tggttgccgc caacagtgtc gccatcccgg tcaatccgag taaaagcgta atttcttcgc
     3001 catcagggaa ccataatccc aggctacgac catacaacat gccaatggca agaccgataa
     3061 atagcgtcgg tgtaaagaca ccaccgggtg cgccggaacc gctactcgcc agcacggcac
     3121 acagtttaca aaggaagatc ccggcaatga tcattaacag tggtggggcg gttaaaaagg
     3181 attgtacggt gctatagccg ttgccccaca ctgcaggtgt gaacagggaa agcagaccca
     3241 cgatcaaccc gcctagtgcc agttgccagg gcggcgcaag tttgagactc acaaatccac
     3301 gatgacaggc gttcattaac gttaacaaca gtggtccgca cagacctgcc agcacacctg
     3361 tactgataat cagcgcatag tcacgagcct gaaccgtcac tgagagttgt acgttgtaga
     3421 gtaacgcgtc gctatgatta atcagattgc taaccagcca tgccacgatg gcggaaataa
     3481 tcaccgggcc gagagaggcc aacatcatag tgccaaacag cacttcggct ataaataaac
     3541 tgccagcaag cggggcacga taggccgcag ccattcccgc cgcggcccca caggcgatcc
     3601 ataatttcca ttcctggcgt ggcgtaaaac gttgggcaaa acaggaggcg gcaagggcag
     3661 ctaaaagaat catcgcacct tcgcgaccaa ttgcactgcc gctggttact accagcagcg
     3721 aggcaagcga tttaaccagg cttgctgcgt aatcgaactg tccatcggtt tgcaacgctt
     3781 ccatgtaatc ggtcggcgca tgagggcgtt gttgggtaaa tttctgccag cccatcagca
     3841 acaaacccgc cgccagtccg ccgagcgccg gagttagcag ccgtcgccag gaggaaaggt
     3901 ttgtcgctgc attgaccaga ctgccggagt cattattgag gaacaaccac tccagtagca
     3961 gcatcgcatg acgaaacccg gcaacggcaa agacagcgag aataccgacg actgttgcga
     4021 taagcagacg gcggaacata gtgcgcagat ccggatagat gtgtagatga tgcatgagca
     4081 gaaatgggca atgaaacacg ggcgactatt ttgcggcaaa agaggccaac cgcaaaagca
     4141 gtgcgtgctt taagagataa aaaaacgtga cattgtaacg tattattgcc gaatcgcgcg
     4201 taatcgtcac catccggcaa tattacggtg atcctatcga aagagtggtt taaccgcgac
     4261 aggaatcaac agttgtgatt gccattgcgc cagcgttaag cgagccagtt cacccagtgc
     4321 acggtagaaa gggtgttctg ctttttcgat aaaaacatca agaaaacgtg ttgaccacgg
     4381 aaaaaggtgc catgccagca attcttcgca ttctgtctgg cgaccattct ccgccaacca
     4441 cgcagccatc aacagcagtg aaccaaaatg atcttccggt tcgttttgct tcatttcaaa
     4501 ctgaatgcct ttctcgcgca tccactgacg aagtgccaat gttgaatcgc caaacagcac
     4561 agattcgcga tccagccaga ccgaccccca cggcggagac ggcagtgccc acgggccgac
     4621 aaacaaacgc tgccaggcct gggcgtgagt ctcttcacac tgtgtctgaa aagccgtcac
     4681 cagcggcgct aatgacgctt ctggtagagg ccactgagtt tcccagccgt cactggtgag
     4741 taccgcaacc agaggtgcgg cttccgcact ctctggagcg taataaaaca gcgcgcccaa
     4801 cacgcgcgcc gcgacagaaa aattatcttg ctgtgaaaaa tgggtcattc ctaacatcct
     4861 gaaatgtgcg ggttgccccg caccaatttg ttaacctgca atagccattc ctactgtcat
     4921 gtgcaggcca taaaagagca cacgcccgat catctcacca acaagtatga ggattagccc
     4981 cagaacaagt ccggcaacgt gaggttcacg gcgacgaatg agcggacaaa gccagcatcc
     5041 aagacctgcg cataacaata ccacacgcca gacctgtaag gaggcgtaat ctggtaccag
     5101 tgcgctggct tgttgcacgg atgagtgaat agacgccaga gacaaacctt gcagcacaat
     5161 cacctcggca caagcgatca gcgccagaac gctaatgatg gcaaatggtg tggtattaaa
     5221 tgtaacgcgg gctgcccgca gaattgcggc agcaagtatt ggaccgctca acaataccgt
     5281 caggaagaaa gccagcgtag tgtaaccgtt atgccaggtt ggcacggtgt cgatttgata
     5341 cacgcaggtc atcatccaga cgaaaatgac gccgagcgcc atgctgacaa gtaaccagag
     5401 tttccccaac gcttgcggca ttttaccgat gaccgccacc agccaccata gtccgccaac
     5461 ggcaaaaaat atggaaccgg cagcaatctc gttactcaga ccagaagctc cgattcgatt
     5521 cagcgagtta aacgctcgca gtggcgatcc caggtgcatg acagaggcaa taaacccgat
     5581 gcccatcaac agccagagga aaaacatgcc acgaacaatg tgctgacggt ctgcgtcatt
     5641 tttggcggcg aaccagccaa ttccgctaac aatcagcgcg cctactacgc attgacccaa
     5701 tacggtaaag ataaccagtg gccattcatg ccatccattt cccattttac acttcctccg
     5761 gattagccag ataaccagtg gtatcgccgg tcgggcggct gttggcgtta ggtttgataa
     5821 cgatattggg ttttgtgaaa tgcgcgcgcg gcagtggcgc gacggcagcc agagtgccgt
     5881 gcttctggcg cagctcttca attggaccga actcaagtgc acgcagcggg caggattcga
     5941 cgcatatggg ttgtttcccc tcggcgacgc gcgaataaca accatcgcac ttcgtcatgt
     6001 gccctttttc agcattgtac tgtggtgcgc cgtacggaca agccatgtgg cagtagcggc
     6061 agccgataca aacatcttca tcaaccacca caaagccatc ttcacgctta tgcattgcgc
     6121 cgctggggca gacttttgta catgcggggt catcgcaatg gttacaggag atggagagat
     6181 aataagcgaa cacgttctgg tgccagatgc cattatcttc ctgccagtcg ccgccagcat
     6241 attcataaat acggcggaaa ctgacttccg ggccaagatc tttaaaatct ttgcacgcca
     6301 gttcgcaggt tttacagcca gtacagcggc tggaatcgat aaaaaatcca tattgtgtgg
     6361 tcatgggcta ctccttaaac cttttcgatc tggacaagat tgctgtgcga cgggtttccc
     6421 tttgccagcg gtgaagggcg gtgagaggtc agaatattga tactgccgcc gtgatcgacc
     6481 cggtcaccaa acatatccgc tttaagccac gcaccttgcc cgatggcggt aacgccaggc
     6541 agaatacgcg gagtcacttt tgcggcaatc agcatttctc cattattgtt aaatacccgc
     6601 acggtatcgc catgacggat accgcgtgcc tgagcatcaa tggggttgat ccacacctct
     6661 tgtgggcagg cctgctgtaa cacatcaata ttgccgtagc tggagtgggt acgcgctttg
     6721 gtttggcgac ttcactcagc atccagtaga tgggtttgcg ttcaaatttt gctgaggttg
     6781 cgggttgggc gaggatcaca tagcccatat tccctgcaga ttcatgagag ataaggtctt
     6841 cttgctctgt tggcatcagg tcaggcaaca ggatatcgca atacttagcc gaggccgtca
     6901 tgaagtggtc aatgccaaca atcatctcgc acttgctgtc atcctgaagc acctcatggg
     6961 tgtgattgat gtcgccatgt tgattgatca atgtgttact ggcgtagcac cataaaaact
     7021 tgatggggac atccagtttt tcttttccac gaacaccatc acgggtcgcg gtcattttcg
     7081 taccatggtc gatggcatct gtccatgtaa aaacggaaat ctgcgtttta acaggattct
     7141 cgagcatcgg gaaccattct acccccagat cccagctacc ttcgcgtaca cctgagttgc
     7201 cgccgtttat gccgacgtta ccggtaagaa cggaaagcat ggcaatagcg cgggacgttt
     7261 gttcgccgtt ggagtgtcgt tgtggccccc aaccctgaca aatataagca ggttttgctg
     7321 aaccgatctc tcgtgccaac tggataattt tttctgccgg gatgctggtg atttttgctg
     7381 cccattccgg cgttttagct atgccgtcag gcccttcgcc cagaatatag gctttataat
     7441 gcgcgttacg tggtgcgttg gcgggcagcg ttttttcatc gtaaccaaca caatatttgt
     7501 cgagaaatgg ctgatcgacc atgttttcag taatcagtac ccaggcaatc gcacaggcca
     7561 gtgcgccatc ggtgccaggg cgaatgggca gccattcatc ttcacgcccg gcagctgtgt
     7621 cgttatagcg tggatcgatg acgatcatgc gtgcgtttga acgttcgcgg gcttgctcga
     7681 cgtagtaagt gacaccaccg ccgctcatcc gcgtttctgc cgggttattt ccgaacataa
     7741 cgaccagttt cgtattggcg atatcatccg ggctgttgcc atcattggca ccgaacatat
     7801 aactcattgc ggcactgatc tgtgcggtac tgtagctgcc atagcgactg agaaaacccc
     7861 cgcaagagtt catcagacgg tacgggacgt tcgagttggt gatatttccg ccatctacgc
     7921 ctgttccgta caggacatgt acagcctcat tgccgtaatc tttcaggatc cgccgaagat
     7981 tatcactgat ggtatccagg gcttcgtccc aacttatccg ttcaaattta ccttcaccgc
     8041 gctcgccgac gcgcttcatg ggatatttca acctgtcagg atgattcatc cgtcggcgga
     8101 tagagcgccc gcgtaaacat gcccgaacct gatgattacc gtaaacgtcg tcacctgtcg
     8161 tatcagactc cacccagtac acggtgtcat ctttcacatg caaacgtagc agacagcggc
     8221 tcccgcagtt aacggtgcag gaactccaga ccgttttctc ttctaccgga gcctctgccg
     8281 cccggaccat ttgggaaaat ggcagagtga aagcactgct tgccagcgcg agactgccaa
     8341 gtgcggaggt tttcatcaga cttctatggc tgatttcagc cttcatgagc gcctctgtgg
     8401 tatggatttt catcattact cacttattgc ttttcaaaca aaatgtcatg ccagaattta
     8461 tggttgtagt gggttatatt ttttcgatct cgaccagatt agtgtgctgc gggtttccct
     8521 tcgccagtgg tgaagggcgc agagtggtta gcgtattcac acatccgcca tggtcgattt
     8581 tatcgccaga catattgggc tcgtgccagg ctccctggcc catagcgcta actccaggga
     8641 gaatgcgtgg tgttactttg gctggtagcc gaacttcgcc acgatggtta aacacccgca
     8701 ccatatcgcc gttggcaatc ccacgtttct gtgcatctat agggttgatc cacacctcct
     8761 gacggcaggc tgccttcagg acatcaatgt tgccgtaggt cgagtgagta cgggatttgt
     8821 aatggaaacc aaacagttgc agtgggaagg ttctacgttc aggggagtcc cagccttcaa
     8881 aggttgaggc ataaactggc agtgggctta tcacttcatc tttttccagt tcccaggtac
     8941 gggcaatttc cgccagcttg ctggaataaa tttcaatctt accggaaggc gttttaagtg
     9001 gatttgcctc ggggtcgtca cgaaatgctt tgtaggcgac aaaatggcca ttgggatctt
     9061 tacgcttata gatacccatt tttttcagtt cgtcgtaaga cggtaacgcc ggatctttgg
     9121 caagcatttt ggcgtacaga tgttgtaacc attgttcctg cgtgcgacct tctgtgaact
     9181 tttgatagac gtcaggtcca agacgtttcg cgacttcact caggatccag taaatcggtt
     9241 tacgttcgaa tttttcgctg gtgactggct ggaggaaaat gagatatccc atgttaccgg
     9301 cgtagtcgtt aggaataata tcttcctgct caacggtcat caggtcttcc agcagaatgt
     9361 cggcatattt tgccgatgag gtcataaagt tttcgatgac cacaatcatt tcgcatttcg
     9421 attcgtcctg cagaatttca tgcgttttgt tgatgtcaga atgctgatta acgagggtat
     9481 ttcccgcgta gttccagatg gactccactg tcagacggat ggacggactt gaagtagttg
     9541 actcaacgct acttaaggtc ctgatatgac tgacattaac catgacaaca ctgcatatcc
     9601 atccagttat ttctctgggt ctccctatct gtatgggcaa gtctacaccc caagtcctga
     9661 cacaaggctg acacaaatca atctactcgg cgtagctaag cctcagttag tccgtagagc
     9721 caatggtcga tatactatcc gcttcagact caagggacag accacaccgt ttctttcagt
     9781 atcgacccgc agcacagaca ggagagtagc cacaatgcgt cagagagagc ttgcagccac
     9841 agctaaggcc ttcatgttgg atagacctga agtctcacta caggagctta cagagcatct
     9901 ccggtctatg gctgagcagt tcctgacaga cgctagtgac gattactgga atggacttga
     9961 ggtcgctact ctggtcgacg agaagtccaa cctgaaggag ctagccgcta cgcaagcctt
    10021 gagtctggac cagcagaagg gcataaggtt agcgctggaa gtcctgaccg cagcacagca
    10081 gagagtcgac actggtgata cctctggtct gattaagctg attgatgata ataaccatac
    10141 tgattactca actataggcg actctacgtc aattctgaag aatgagcagg gggatagacc
    10201 agtagttttc acacaggagc gccaacctga ggtcactatc tgtagttttt ctgagctagt
    10261 gtcctcgctg ctggctgaga aggtccagac cctgaagtcc tccagctaca aggacctctc
    10321 gtcctcacta aatactgtct cccggttcct gcctgaagac atggacctga tgtctcgctc
    10381 tgagtggctg gctgtcaggg acgctatgct ggcttctgag gtgagaccgt cgacgattaa
    10441 caagctgctg actaaggcga agatgtgtct ggactatggc ctgatgaatg ggcaacttga
    10501 gggccgcaac ccgattgagc gcatgaagtt aactaaggat gtagactcca agcgtagagc
    10561 attcactgac gaggagatgg agagacttct ggtccgtgtt gagtctgagt atcagttcac
    10621 acgacacacg gctcacacga cctctgaggc ccgtagatgg gcgtctctgg tgtctgtagt
    10681 tactggcgct cggtctgctg aggtctgcca cctgactaaa cgtgacattg taaccttgga
    10741 caatgggctg gtctgtatcg acatcaatga agatggggac ggtaagtctg tgaagaacaa
    10801 gcattcagtc cgacttgtgc cgttgaccga tggggcctgt ggtcttgacc tgaagtcctt
    10861 cctcgaatgg gtagacatgc agcctgacga gggtcctctc ttcgggatga ctccaagcgc
    10921 atactctagc tggtttaact caagggtcct gactgaagct ctgggagacg ctacggatgt
    10981 ctctctccac agtctgaggc actggctagc gaccagaatg aaagagcgtg gagccaatct
    11041 ggtggacgct cagggcatct taggtcacag cagtcagtcc attacgtatg acctgtacgg
    11101 gaaaggacac gctgttggta ggctggctga ggtgctgaag acagctttac tttaagtagg
    11161 aagacccaag gtgggagacc atatacggct tgatggttga gaccttgggt cactgaagtc
    11221 tgactgaagt tagggagggt gataataatc ataatccctc ctgactatag gcccactggt
    11281 tcgccttcgg ctgtccgact ctaagtctgt ctgtggtctt atttaaactg gttccctaac
    11341 tcaggtcctc caacttggtg tcttacttaa actggttccc taactcaggc ccctccaaat
    11401 ttcgttgtct gtcagcgact taaatatccc ctcaccttag aagagtttag ggttcagacc
    11461 cttaactatc accttaggag agacgggccg tcctccaccc aatcaaaacc ttaagaggag
    11521 gaccatacat ggctattaaa attcagtgct acaccgagtc cgacaatgcg gctttcttcg
    11581 gtgacgattc cgaccaccag caccagctac cagcaccgac atatgacgaa cctaaggccc
    11641 gcacaggtaa
//
LOCUS       DAZXUK010000116        11517 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZXUK010000116 DAZXUK010000000
VERSION     DAZXUK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11517
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.116"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(209..661)
                     /locus_tag="ACH3L8_002430"
     CDS             complement(209..661)
                     /locus_tag="ACH3L8_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFT4085506.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(714..2048)
                     /gene="adeQ"
                     /locus_tag="ACH3L8_002431"
                     /gene_synonym="yicO"
     CDS             complement(714..2048)
                     /gene="adeQ"
                     /locus_tag="ACH3L8_002431"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFT4085507.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            2223..3989
                     /gene="adeD"
                     /locus_tag="ACH3L8_002432"
     CDS             2223..3989
                     /gene="adeD"
                     /locus_tag="ACH3L8_002432"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFT4085508.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(4035..5426)
                     /gene="uhpT"
                     /locus_tag="ACH3L8_002433"
     CDS             complement(4035..5426)
                     /gene="uhpT"
                     /locus_tag="ACH3L8_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFT4085509.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(5564..6883)
                     /gene="uhpC"
                     /locus_tag="ACH3L8_002434"
     CDS             complement(5564..6883)
                     /gene="uhpC"
                     /locus_tag="ACH3L8_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFT4085510.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(6893..8395)
                     /gene="uhpB"
                     /locus_tag="ACH3L8_002435"
     CDS             complement(6893..8395)
                     /gene="uhpB"
                     /locus_tag="ACH3L8_002435"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFT4085511.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(8395..8985)
                     /gene="uhpA"
                     /locus_tag="ACH3L8_002436"
     CDS             complement(8395..8985)
                     /gene="uhpA"
                     /locus_tag="ACH3L8_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFT4085512.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(9060..9350)
                     /gene="ilvN"
                     /locus_tag="ACH3L8_002437"
     CDS             complement(9060..9350)
                     /gene="ilvN"
                     /locus_tag="ACH3L8_002437"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFT4085513.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(9354..11042)
                     /gene="ilvB"
                     /locus_tag="ACH3L8_002438"
     CDS             complement(9354..11042)
                     /gene="ilvB"
                     /locus_tag="ACH3L8_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFT4085514.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(11148..11246)
                     /gene="ivbL"
                     /locus_tag="ACH3L8_002439"
     CDS             complement(11148..11246)
                     /gene="ivbL"
                     /locus_tag="ACH3L8_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFT4085515.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            11233..>11517
                     /locus_tag="ACH3L8_002440"
     CDS             11233..>11517
                     /locus_tag="ACH3L8_002440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085516.1"
                     /translation="MEVVIGDLVGFVFNNIYTSTALQGRQWKIFQQGKIEGLITFCTE
                     LQITKNPAGARFRQPPAAGNRKAHCIMSTLKVYVFRAERAISARILTYLVH"
BASE COUNT         2954 a         3230 c         2905 g         2428 t
ORIGIN      
        1 tgttacccgt ggcatgtttc aggggaaaag ccgaccactt taacattgaa atgtgtgacg
       61 aatttaacgt ttttgcaact tttacggtgt tgcttgagct ggagttaaga tcgaaacgga
      121 acagggcaaa gattaccgga tgcggcgtga acgtcttatc cacccgacag gtcaggcgcg
      181 gtctgtaggc tgataagacg aaacggtttc aatgcaacca gtgaacgccc tcggcaggct
      241 gaaagaacgc gttatagcgc atctgaaaag cattaatatc ctgcatttca ggttcggttt
      301 cacgcagaaa acccagcgcc agcctgacct gagcatcggt aatcggcgcg gaaaggcggg
      361 ctttttgtaa aatccggtgc cattgcagca ggttctgttc gctatcatcg acaatgacaa
      421 tttgccagat aagcaacacc tgcgcggcgt tttgcaggtg cgactcatcc gggcgatgca
      481 aataacgtaa aaattcgtca ccggctgact ttcccatttg atcgaccatc gactccacgg
      541 gtacgacatc gatattcagc cgatcgctga gacggcgaat cgcttttctg gccccggatg
      601 tcagcacccg aaaaccaacc acaaacacta ccgccagcgt ggcgagcatt atccagatca
      661 tgagttctcc tgcgtaatca gaactcatga tatctggaaa ccctcgccag ttactaatcc
      721 accagaataa tcttcaatgc gaacagagct gcgaccacca ccacacacag gttcagatcg
      781 cgccagcgcc cggtgcacac tttcatgatg cagtacgaca taaagccgag tgcaatccct
      841 tcggtgatcg agaaagtaaa gggcatcatc accgtggtaa taaacgcagg caccgattcg
      901 gtaaaatcat cccagttaac gcgcgccagg ctcgaagtca tcagcacgcc aacaaagatt
      961 aacgctccag cggttgcgta aggaggaacc atcgccacca gcggtgagaa gaacataact
     1021 aacaggaaca taacgccgac cacgacagcc gttagccccg tgcgaccacc gactgccaca
     1081 ccagaagtac tttcaatata ggcagtaaca gacgaggtac cgataaatgc gcctgccacc
     1141 gaactgacgc tatcaacata cagcgcctta ttcatattgg ggaatttacc gttgctatct
     1201 attaagcccg ctttatcagt tacaccaatt aatgttcctg atgaatcaaa taggttgatc
     1261 agcataaagg agaaaatgat accggcgagt tcaagtgtta acgcgccgct caaatctact
     1321 tcaccaatga cgccgctaat atcaggcgga atggaataga cgccgctaaa atgaacatca
     1381 ccgaaaaata atccacagca agacgtcacc acaatagaaa ccagcaccgc ggcatgaaaa
     1441 tgacgtgatg acaacacggt tataataaaa aaccctaaaa tacctaacaa cacgccgtga
     1501 gaacttaaat cgccaatcat caccagcgtg tctttattgg cgacaataac accagtgttt
     1561 tttaatccca ttaaggcaat aaataatccg attccactgg tgataccaat acgtaaactt
     1621 aatggaatgt tggagatcat ccagtaccgg atacgaaaca gcgtgagcaa aaatagccca
     1681 attgcgcccc agaatatcgc gcccatcccg gtctgccagg agatccccat cgcccccacg
     1741 accacgaacg caaagaaggc gttcagcccc attgccggag ccagcgccac gggtaagtta
     1801 gcaaatatcc ccatcgcaat actgccgata ccggcaatca aacaggtggt aacaaacacc
     1861 actttcgggt ccatttgtgc cgcgccgagg atttgcgggt tcacaaaaac aatgtacacc
     1921 atggttaaaa aagtggtcat ccccgcaatc aattctgtgc ggacggtggt cccatgctga
     1981 ggtagtttaa ataatcgcga aagcgtccct gattcattac tcacgtaatc ggtattgtca
     2041 ttattcattt ttttgtccat tggaggagat ttaatccctt gcttttgtaa aacaaatgac
     2101 aacaacggtt cagtgataat tatcacattt caattgcaca ttaatagata ttctttaata
     2161 atctcgcgac gtttctttat gataaataat aatcaaattg ataaaatcaa aatgagaaaa
     2221 atatgaataa ttccattaac cataaatttc atcacattag ccgggctgaa taccaggaat
     2281 tgttagccgt ttcccgtggc gacgctgttg ccgattatat tattgataat gtctctattc
     2341 tcgacctgat caatgccgga gaaatttccg gcccaattgt gattaaagga cgttacattg
     2401 ccggtgttgg cgcagaatac gctgatactc cggctttgca gcggattgat gctcgcggcg
     2461 caacggcggt gccagggttt attgatgctc acctgcatat tgaatccagc atgatgacgc
     2521 cggtcacttt tgaaaccgct accctgccgc gcggcctgac gaccgttatt tgcgaccctc
     2581 atgaaatcgt caacgtgatg ggagaagccg gattcgcctg gtttgcccgc tgtgccgaac
     2641 aggcaaggca aaaccagtac ttacaggtca gctcttgcgt gcccgctctg gaaggctgcg
     2701 atgttaacgg tgccagtttt acccttgaac agatgctcgc ctggcgggac catccgcagg
     2761 ttaccggcct tgcagaaatg atggactacc ctggcgtaat taacgggcag aatgcgctgc
     2821 tcgataaact cgatgcattt cgctacctga cgctggacgg tcactgtccg ggtttgggtg
     2881 gcaaagaact taacgcctat attgctgcgg gtattgaaaa ctgccacgaa agttatcagc
     2941 tggaagaagg acgccggaaa ttacaactcg gcatgtcgtt gatgatccgc gaagggtccg
     3001 ctgccagcaa tctcaacgca ctggcaccgt tgatcaacga atttaacagc ccgcaatgca
     3061 tgctctgtac cgatgaccgt aacccgtggg agatcgccca tgaagggcat atcgatgcct
     3121 taattcgccg cctgatcgaa caacacaatg tgccgctgca tgtggcatat cgcgtcgcca
     3181 gctggtcgac ggcgcgccac tttggtctga atcacctcgg cttactggca cccggcaagc
     3241 aggccgatat cgtcctgttg agcgatgcgc gtaaggtcac ggtgcagcag gtactggtga
     3301 aaggcgaacc gattgatgcg caaaccttac aggcggaaga gtcggcgaga ctggcgctgt
     3361 ctgccccgcc atatggcaat accattgccc gccagccagt ttccgccagc gactttgccc
     3421 tgcaattcac gcccggaaaa cgctatcggg tcattgacgt catccataac gaattgatta
     3481 cgcactcccg ctccagcgtc tacagcgaaa atggttttga tcgcgatgat gtgtgcttta
     3541 ttgccgtact tgagcgttac gggcaacggc tggctccggc ctgtggtttg ctcggcggct
     3601 ttggcctgaa tgaaggcgcg ctggctgcga cagtcagcca tgacagccat aatattgtgg
     3661 tgatcggtcg cagtgccgaa gagatggcgc tggcggtcaa tcaggtgatt caggatggcg
     3721 gcgggctgtg cgtggtacgt aacggccaag tacaaagtca tctgccgtta cccattgccg
     3781 ggctgatgag caccgacacg gcgcagtcgc tggcggaaca aattgacgcc ttgaaagccg
     3841 ccgcccgtga atgcggtccg ttacccgatg agccgtttat tcagatggcg tttctttctc
     3901 tgccagtgat ccccgcgcta aaactaacca gtcaggggct atttgatggc gagaagtttg
     3961 ccttcactac gctggaagtc acggaataat aaaaaaagcc cggcgtcatg ccgggcaaaa
     4021 gtcaccagtt acgtttatgc cactgtcaac tgctgaattt ttttctcgcg gcggattttg
     4081 cgttcttcca ttaccgccac tatcgccatc aggcagatac aaccaatcgc ggcgatatcc
     4141 agcgcggcga aggtgcctgc ccagccggta aggccgaata ccggcgtccc atcggcaatc
     4201 attcccagac ctaacttggc aaagctgtca ccaatcaggt aagcaaaggt gcctttaata
     4261 ccatcggcag cgccaatcgc ttttttaggt acaaagccaa cagcagccac accaatcaac
     4321 aattgcgggc caaagaccag gaaacccaac gcaaagagag aagccagata gatatattcg
     4381 ttactggcat gttgatacac accgagcgtg gcgataatca gcgccagcgc gatgcaggcc
     4441 accaggccac ggcgaccgtt cgccaggtca gagagccagc cccacagcag cgtaccgacc
     4501 agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca ccgctttaga gagtttcagt
     4561 tcctggaacg cgtatacggt tgaccactgg tcgataccaa tacgtaccac atagaggaaa
     4621 atgttggcga agcacagcag ccagatcact ttgtttttca gcacatactc aacaaagatc
     4681 tgccacttgg tcatatcggt agattctgtc tctttgtcct cttcgctgat ctcctcgccg
     4741 aacagttctt cagctttgcc gaggccataa gattccgggg agtcgctgcc gtaacgcagg
     4801 ccgataaaac cgacaatcag cgcgataatc gacgggaaga taaacatgcc gatgacatgg
     4861 ccatcgaaca ggtaatttgc cccgaacagc gccacacctg ctgcgcctgc accgccaagg
     4921 ttgtgagaaa tattccagaa accgaggaat gtcccgcgtt tacgacgcgg cgtccatttg
     4981 gtgatggtgg agtaactgca cgaaccgccg gtactctgga aaaagccgct taaggcgtag
     5041 aaggcaatca tcaggaacag gctaaccgag ccgctgccca tactggcact gaagcccagc
     5101 atacaaatgg cagagaggat aagcatgaac ggcaggaatt gtttggtgtt tttgccgtcg
     5161 gcgtagtatg aaaccaacgt tttacccacg ccataagtga tggagaaacc caggccgatc
     5221 atccccagct gcgtcatgct caacccgtag gtcgaaatca tatcgttctg cgcgatgtta
     5281 aagttcttgc gaatcaggta catcgtcagg tagccgataa agaccaccag gtaggattgc
     5341 atgaacggtt tgaaccacat tttgcgccgc acttcgagcg gaaggtccag ggtcggcttg
     5401 cgaacttggt ttaggaaagc cagcatgggt tactcctgaa atgaatacct gcgtgaggca
     5461 tgcattgtaa aaatcagcag caaatcaggc ctgagatagc gtccaagaaa aacctggaaa
     5521 atttcttagt tttgccggat atgaagtgaa aaggtgagat gcatcacgct tcacgcggtg
     5581 tctgggcgtt caaaaagggc agtaacagca gtgcggaaat cccggcggcg atagagataa
     5641 ccacaaaaaa tccgctccag tgccaggtat cgagcacttt cgccagcggc caaccagcaa
     5701 gcgacgcccc cagataagca aacaagccga caaaccccgt cgccgcccct gccgcctctt
     5761 tgtgggaaca ctctgccgcc gccataccaa ttaacatctg cgggccaaag acaaaaaaac
     5821 caatggtgaa gaagcaggtt gcctgcatca cgtagctggc aaatggcatc agccacagcg
     5881 agccgactga aagcaaaatt ccggcggcga aaatcaaatt catcggccca cggttgccgt
     5941 taaacaattt gtccgagccc caaccggcta ccagcgcacc gataaatccg cccagttcaa
     6001 acatcgtcac tgccgtattc gccgtgacca gatcgacgcc cagcgtctcg gacatataca
     6061 aattgcccca gtcgttgatc gccgcccgga ccacatagac cagcacatag caaaacgaaa
     6121 gcagccagat atacggattc agcaacacat atttggtgag gatctcttta cgcgtcaacc
     6181 ctgccccttc ttgttgttga gcaatttcca gcgcgtcgtg ccgccattca ccgaccgccg
     6241 gtaaacctaa cgcctgcggg cgatcgcgta gccgccagca gagaaaaatc cccacgacta
     6301 tcgccataca accagcaatc atcatcccgg cacgccagcc gtaatgcagc gcgctcgctg
     6361 ccatcacaat gggaatgagt gcgccgccga cgttatgcgc cgtgttccat aatgcccacc
     6421 aaccgccgcg ctcggtacgt gaataccagg ccgttaacag acgcgcacac accggtgaac
     6481 cccagccctg gaaaaaggcg ttcagcaccc agagcacggc aaacgcccat agcgacgtcg
     6541 agaagccaaa cagaatgttc atgatgcccg tggcgataag ccctatcccc ataaaataac
     6601 gggcatttga gcgatcgctg acaatgccgg agacaaactt cgacacgccg taagtaatgt
     6661 aaaacagggt cgctaacagg ccgatatcgc tacggctgag cacgccgtta gcaaggattt
     6721 ctggtaccgc ggcgttaaaa cttttccgcg tgaagtaaaa cagcgcgtaa cccagccaga
     6781 tggtcagcag aatatgccga cgccagtagc gataacgggc atcaatttca tgtttatcag
     6841 tcattaatgg cgcatcggca ggcgctttca gaaacggcaa catcatcgaa ccttagacat
     6901 aacgttgagg tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg tgtaatgtgc
     6961 cgcccagcgc cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt tgcccggaac
     7021 ccggcggcaa accgctgcca tcgtcttcaa tcaccagcat caaccgttca tcctgctgcc
     7081 agccttgcag cgtaaccgcg ctggcatcgg catgtttcac aatgttgttc agcccttcct
     7141 ggcagacacg aaacagcgtc acgcgctggt tttcgcttaa tgctgattca tcgatacgcc
     7201 attcgagatg gctgacaatg ccgcgccctt ccagctccat ttcccgcatc agtgagcgga
     7261 tggcctgctc cagggtgaga tcatccagct ggcgcggacg taaccgtcct aacaaacggc
     7321 gcaccgcgtc gtaaacgccc agcgacagtt gttcgatgag ctgcccgctc tgcttcacac
     7381 tggcgttatc tgccgccagc cgctgaacaa tgcccgcctg agtacgaata gcagtgatgg
     7441 tctgaccgat atcgtcgtgc agctcacgcg ccacgtcgcg gcgcacgctc tcttcggttt
     7501 ctaacaaacg ttcagccaga tgctgattgc gcgccagttc cttttgtagc gactggttaa
     7561 gttcacgcaa ccgctggatg cctgcgccca gtaacaaccc tgtcagactt tgcaccagca
     7621 acgagagtaa taaatccacc agatgatcgc gccaggtttg actggcgatc agcgcgatgg
     7681 cgttcatcaa cgtcgcaatc agcgctcctt gccagccata gtgccaggcc agcgcgataa
     7741 tcggcagcgc cagacagaat ggcgtaaagc gcgacagttc gtccggcaat cccaactgga
     7801 gccagagact gataacaaac agcagcaagt accagaccag atgccgcccg cgccagttga
     7861 tgggctgaga aaccagtgac gggccgagcg gcagccaggt gttattggcg agatagtgcc
     7921 agaacaccag acatatcggg gccagcgtca gaccgccagt taaagtcagc aacagcgcat
     7981 tccacgactc tttgccgtgc caaagccagg gcagcgactg caacaacgcc gccgccgtta
     8041 acgccgcacc ctgtaacagc aaggtgcgcc agtcacgctg atggcgatag cgcgagatca
     8101 gcgctacggg cagtaacgtc agcaaactac cgatcatcaa taacggaaaa tgggttaaac
     8161 cgaccgcctg cgttagccag taaatcagca gccactccgc acccagcaat acgggccagt
     8221 atccgcgcgg gcattgcagc attagcccca gacgcagacc aaacggaaat aacagcaccg
     8281 ccatatcagg gcgctcaacc agatgcaggc tgatactcca taggcaaaac catgcggcag
     8341 agaagataaa aaagcaggca ataacggtaa ttaagcggga gaacaacgtc ttcatcacca
     8401 gccatcaaac atgcggcgcg ccagctctac gtcgttactg acgcccagtt tttccatcag
     8461 attggcgcga tggacgtgta ccgttttcgg tgacaagccc agttcggcgg caatctcttt
     8521 caccgccatt ccttgcgcca gtttttccgc cacctggcgt tcacgtttgg tcagcgggtc
     8581 ctgacgaccg gatgccagtt taatggcaat atccggcgtc agataacagc cgcccgtggc
     8641 aaccgtatgc accgcagcaa tgagttcatc cgggctacag cgtttggaaa gaaagccgcg
     8701 tgcccccgcg ttaagcgcct gctcaaccag cgcaggactg tcgtgaacgg agagcataat
     8761 cgtcgccata cctttcggca gctggcttag cagctccaga ccggagatat cgggcatgga
     8821 gatatcgcaa atacacacct gcacaccgcg ccccggcagc cccgccagcg cctcgcgccc
     8881 cgaaccaaac tcggcaacta cctgcaaatc aggttccagc cccagcagct gcgcaaagcc
     8941 ggagcggacg atgaggtgat cgtctataag ggcaacggtg atcatggtct tgtcctggcg
     9001 ggtaaaaaaa aacgcgctta ccttaacgat aagcgcgatg ttgttcaagc cttgagcggt
     9061 tactgaaaaa acaccgcgat cttgttaaac atcgtcggat cggactgatt acgctgcact
     9121 ttcacgacat cttccagctt agcgatttgg cttatcatct gctccagacg ctggtcgtca
     9181 ttgaccagta gccagatatg gcttttgtcg ctgtcctgaa tcggcagaca aagaatgcct
     9241 tcaacgttaa aagcgcggcg ggcaaaaagg ccacaaacgt gggtcattac gcccggatgg
     9301 ttgcgaacgg tgagctccag aattacgttg ttatgagttg tgttttgcat ggcttattcc
     9361 cccaccattt cagtattcgc cgcacctggc ggcaccatcg ggtaaacttt ttcttcggca
     9421 tcaatgcgca catggatcag cgccgggcca gggcgattga tgatttcctg caatgcagcc
     9481 tgcggatcgg cttcgttatt caaatcacag gtttcgaggc cgaatccggc ggcaatctgc
     9541 ataaagttga ttttgcccgg ataggtggcg gcaaaaacgc cttgctcgta gaacagactc
     9601 tgttgctgat gcaccagccc cagcgcttcg ttgttcatca gaatgatttt gacatccagc
     9661 tgattttcac tggcggtcgc catctcctga atattcatca tcaggctgcc gtcgccggag
     9721 aaacacaaca ctttgcgatc cgggttcgcc agcgccgcgc caatcgccgc aggcaggcca
     9781 aaacccatcg tgcccagccc accggaggtc agccactggc gtgggcgatt gagcggataa
     9841 gcttgcgcgg tccacatctg atgctgaccc acatcggtgg tgataatcgc attgtcatcg
     9901 acacaggcgg caacggcgtt gatcaggccg taatggctta atggatcgca cgctttcggg
     9961 attggacacg ggaattcgcg ctgcaaatcc gctaccaact ggtgccactc tgcacgcggt
    10021 tgcgcttcca cccgcgggat caattgcgcc agcacgtcat caacatccgc ctgaatcgcc
    10081 acatgcggct gcttgatttt acccagctct gcgcgatcga tatcgacatg aataattttg
    10141 gcattcggac agaactgctc ggttttgcca atcgcccggt catcaaaacg cgcaccgagc
    10201 acaatcaaca aatccgcctc ctgcaaaata tagttggtac tgcgcacgcc gtgcatcccc
    10261 agcataccca gcgacaacgg atgcgctttt ggcagcatgc ccagcgccat taaagtcatg
    10321 gtggtaggca gttgcgcttt ctccgccagt tcacgcaccc gcgcaggcgc attgatcaca
    10381 ccaccaccca gataaagcac cgggcgtttg gcagcgttaa tcattgtagc tgcgtcacga
    10441 atgctttctt cgctaaaggc gggtgcaacg gctttttctg ccaccgcggg ctgagcttca
    10501 atctcaaaaa ccgccgtttg tacatcctta ggaatgtcta tccacaccgg gctagggcgg
    10561 cctgattgcg caatgcggaa ggcatcgctc atgacctgtg ggagctcttc gatatgtctg
    10621 accagatagt tgtgtttggt gatggggata gagatgccgt aggtgtccac ttcttggaag
    10681 gcgtcggtgc cgatcatcga ggcgggaacc tgaccagtga tgcaaatcag cgggatggag
    10741 tccagccgcg catcagcaat ggcggtcacc aggttagtcg cacccggccc gctacaggcc
    10801 atacagaccg ccggtttacc gtcggtgcgc gccattccct gagcgataaa ccccgcgccc
    10861 tgttcatggc gagccagaat atggcggatt tgcgtgcttt ggcttaaggc gtcgtaaaca
    10921 ggcaggatag aaccgcccgg aatgcccgtc acaatcttaa tgccctgctg ttccaggaaa
    10981 tgaacgataa attctgcgcc ggtaaagcgc ttacgcgtcg atgttgtgcc cgaacttgcc
    11041 atgctccagt ccttttcttc tgggccgact ttccgggagg tgcttaaacg aaaaaccccg
    11101 cccggtttgc gccggcgggg ttttggaatc gtgtgttgtt ccagtcccta cggcgcattg
    11161 ccgacgacca ccaccacacg cacgacgacc actgcggcgg atggcgcagt tggtagtagt
    11221 tttgcgttga acatggaagt agtcattggg gaccttgttg gttttgtgtt taacaatatt
    11281 tatacaagca cagctttaca ggggagacaa tggaaaattt ttcagcaagg gaaaattgag
    11341 gggttgatca cgttttgtac tgaattgcag ataacaaaaa accccgccgg agcgaggttt
    11401 cgtcagccgc ctgcggctgg taaccgcaaa gcacactgta ttatgtcaac actgaaagta
    11461 tacgtgttcc gcgcagaacg cgcaatttcg gcacgaattt tgacgtattt agtgcat
//
LOCUS       DAZXUK010000117        11425 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZXUK010000117 DAZXUK010000000
VERSION     DAZXUK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11425
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.117"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            318..617
                     /locus_tag="ACH3L8_002441"
     CDS             318..617
                     /locus_tag="ACH3L8_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFT4085517.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(737..916)
                     /locus_tag="ACH3L8_002442"
     CDS             complement(737..916)
                     /locus_tag="ACH3L8_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFT4085518.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            990..2351
                     /gene="pabB"
                     /locus_tag="ACH3L8_002443"
     CDS             990..2351
                     /gene="pabB"
                     /locus_tag="ACH3L8_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFT4085519.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2355..2933
                     /locus_tag="ACH3L8_002444"
     CDS             2355..2933
                     /locus_tag="ACH3L8_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFT4085520.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            3117..4481
                     /gene="sdaA"
                     /locus_tag="ACH3L8_002445"
     CDS             3117..4481
                     /gene="sdaA"
                     /locus_tag="ACH3L8_002445"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFT4085521.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4612..6210
                     /locus_tag="ACH3L8_002446"
     CDS             4612..6210
                     /locus_tag="ACH3L8_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFT4085522.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6214..7764)
                     /gene="yoaE"
                     /locus_tag="ACH3L8_002447"
     CDS             complement(6214..7764)
                     /gene="yoaE"
                     /locus_tag="ACH3L8_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFT4085523.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(7752..7910)
                     /gene="yoaL"
                     /locus_tag="ACH3L8_002448"
     CDS             complement(7752..7910)
                     /gene="yoaL"
                     /locus_tag="ACH3L8_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFT4085524.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8227..9198
                     /gene="manX"
                     /locus_tag="ACH3L8_002449"
     CDS             8227..9198
                     /gene="manX"
                     /locus_tag="ACH3L8_002449"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFT4085525.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9261..10061
                     /gene="manY"
                     /locus_tag="ACH3L8_002450"
     CDS             9261..10061
                     /gene="manY"
                     /locus_tag="ACH3L8_002450"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFT4085526.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10074..10925
                     /gene="manZ"
                     /locus_tag="ACH3L8_002451"
     CDS             10074..10925
                     /gene="manZ"
                     /locus_tag="ACH3L8_002451"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFT4085527.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10980..>11425
                     /locus_tag="ACH3L8_002452"
     CDS             10980..>11425
                     /locus_tag="ACH3L8_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFT4085528.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
BASE COUNT         2816 a         2876 c         2856 g         2877 t
ORIGIN      
        1 attcattatt tatatttata atttcaattt tatctataaa actaactata taaataaaca
       61 taaccatgcc gttgtgttcg ccctttgatt agcaactctg gttttctttc ttagctgaac
      121 aatctggtta tagcgtgcgg gctgaccgtt aattttctcc atcttactgg ataacacacc
      181 atgagctgct acctttaaaa cacctgaatg tcaacaggtt aactcgtgcc atatcgttat
      241 aatccctttg atattagatg caaattaagg tcatatatag ccttatcgat aacatggtta
      301 attttaagga taagaatatg cctgctgtaa tagataaagc cctggatttc attggtgcca
      361 tggatgtatc agcgccaaca ccaagttcga tgaatgaaag cacggcgaag ggaatcttta
      421 aatatttaaa agaactggga gtacccgcga gtgccgctga tattacggcg cgagctgacc
      481 aggaaggctg gaaccctggg ttcacggaaa aaatggttgg atgggcaaaa aaaatggaga
      541 caggtgaacg ctctgtgatt aaaaatcctg aatacttttc aacatatatg caggaagaac
      601 taaaagcact ggtctgagtt aaatttatat cagcataaat gggtcaggac gcttttaatc
      661 acatataaaa agcgtccttt ttctcacaat caaatgatca tcggcttcta taacgaaatc
      721 gatacggatt agtgtgttac tcgtcttcat cctcaaaacg cgccacgatt agctcgccgg
      781 agtggttggc acgcagctct tctgccacca gcgcgattgc ctggccgcta ctcatccctt
      841 gtgccatcag ttcctggatc cgctcgacag ctttttgctg ctgttcatgg atgagtgaag
      901 gtaaacctgc aaacattgtt aactcctgct aaattgttgg cgctaattat ttcatgctac
      961 ccggcacata gccagtagag tcaggactga tgaagacgtt atctcccact gtgattactt
     1021 taccctggcg tcaggacgcc gctgaatttt atttctcccg cttaagccac ctgccgtggg
     1081 cgatgctttt acactccggc tatgccgatc atccgtatag ccgctttgat attgtggtcg
     1141 ccgatccgat ttgcacttta accactttag gtaaagaaac cgttgttagt gaaagcgaaa
     1201 aacgcacaac gaccactgat gacccgctac aggtgctcca gcaggtgctg gatcgcgcag
     1261 acattcgccc aacgcataac gaagatttgc catttcaggg cggcgcactg gggttgtttg
     1321 gctacgatct gggccgccgt tttgagtcac tgccagaaat tgcggaacaa gatatcgttc
     1381 tgccggatat ggcagtgggt atctacgatt gggcgctcat tgtcgaccac cagcgtcata
     1441 cagtttcttt gctgagtcat aatgatgtca atgcccgtcg ggcctggctg gaaagccagc
     1501 aattctcgcc gcaggaagat ttcacgctca cttccgactg gcaatccaat atgacccacg
     1561 agcagtacgg cgaaaaattt cgccaggtac aggaatatct gcacagcggt gattgctatc
     1621 aggtgaatct cgcccagcgt tttcatgcga cctattctgg cgatgaatgg caggcattcc
     1681 ttcagcttaa tcaggccaac cgcgcgccat ttagcgcttt tttacgtctt gaacagggtg
     1741 caattttaag cctttcgcca gagcggttta ttctttgtga taatagtgaa atccagactc
     1801 gcccgattaa aggcacgcta ccacgcctgc ccgatcctca ggaagatagc aaacaagcag
     1861 aaaaactggc gaactcagcg aaagatcgtg ccgaaaatct gatgattgtc gatttaatgc
     1921 gtaatgatat cggtcgtgtt gccgtagccg gttcggtaaa agtaccagag ctcttcgtgg
     1981 tggaaccctt ccctgccgtg catcatctgg tcagcactat aacggcgcga ctaccagaac
     2041 agttacacgc cagcgatctg ctgcgcgcag cttttcctgg tggctcaata accggggctc
     2101 cgaaagtacg ggctatggaa attatcgacg aactggaacc gcagcgacgt aatgcctggt
     2161 gcggcagcat tggctatttg agcttttgcg gcaacatgga taccagcatt actatccgca
     2221 cgctgactgc cattaacgga caaatatact gctctgcggg cggtggaatt gtcgccgata
     2281 gccaggaaga agcggaatat caggaaactt ttgataaagt taataagata ttacgccaac
     2341 tggagaagta agacgtggaa taccgtagcc tgacgcttga tgatttttta tctcgctttc
     2401 aacttttgcg cccgcaaatt aaccgggaac ccctaaatca tcgtcaggct gccgtgttaa
     2461 tccccatcgt ccgtcgaccg caaccggggt tgttgctgac tcagcgttca attcatctgc
     2521 gtaaacacgc tggacaagtg gcattccctg gaggtgcagt cgatgacacg gacgcatcag
     2581 ttatcgccgc cgcgctgcgc gaagctgaag aagaggtcgc tataccgcct tccgccgttg
     2641 aagttatcgg cgtgctgccg cccgtcgata gcgtaactgg ctatcaagta accccagtgg
     2701 tcggcattat cccgcccgat ctgccgtatc gcgccagtga agatgaagtc tcggcggtgt
     2761 ttgaaatgcc gctcgcccag gcattacatc tgggtcgtta tcacccttta gatatctacc
     2821 gccgtggcga ttcacatcgg gtatggctat cctggtacga acagtatttt gtatggggaa
     2881 tgaccgcagg cataattcgt gagctggcgc tgcaaattgg tgtgaccccc tgactatact
     2941 tatctttaca tctacaaaac actacttgag acaatcatcg caatattagt taaatcgcgg
     3001 tttttgatta gtttaattca tgtgaatagt taagccagtc gccgcgttcc ctcttacact
     3061 atgcgctgtt attagttcgt tactggaagt ccagtcacct tgtcaggagt attatcgtga
     3121 ttagtctatt cgacatgttt aaggtgggga ttggtccctc atcttcccat accgtagggc
     3181 ctatgaaggc gggtaaacag ttcgtcgatg atctggtcga aaaaggctta ctggatagcg
     3241 ttactcgcgt tgccgtggac gtttatggtt cactgtcgct gacgggtaaa ggccaccaca
     3301 ccgatatcgc cattattatg ggtcttgcag gtaacgaacc tgccaccgtg gatatcgaca
     3361 gtattcccgg ttttattcgc gacgtaaaag agcgcgaacg tctgctgctg gcacagggac
     3421 ggcatgaagt ggatttcccg cgcgacaacg ggatgcgttt tcataacggc aacctgccgc
     3481 tgcatgaaaa cggtatgcaa atccacgcct ataacggcga tgaagtcgtc tacagcaaaa
     3541 cttattattc catcggcggc ggttttatcg tcgatgaaga acactttggt caggatgctg
     3601 ccaacgaagt gagcgtgccg tatccgttca aatctgccac cgaactgctc gcgtactgta
     3661 atgaaaccgg ctattcgctg tctggtctcg ctatgcagaa cgaactggcg ctgcacagca
     3721 agaaagagat cgacgagtat ttcgcgcatg tctggcaaac catgcaggca tgtatcgatc
     3781 gcgggatgaa caccgaaggt gtactgccag gcccgctgcg cgtgccacgt cgtgcgtctg
     3841 ccctgcgccg gatgttagtt tccagcgata aactgtctaa cgatccgatg aatgtcattg
     3901 actgggtaaa catgtttgcg ctggcagtta acgaagaaaa cgccgccggt ggtcgtgtgg
     3961 taactgcgcc aaccaacggt gcctgcggta tcgttccggc agtgctggct tactatgacc
     4021 actttattga atcggtcagc ccagacatct atacccgtta ctttatggca gcgggcgcga
     4081 ttggtgcatt gtataaaatg aacgcctcta tttccggtgc ggaagttggt tgccagggcg
     4141 aagtgggtgt tgcctgttca atggctgctg cgggtcttgc agaactgctg ggcggtagcc
     4201 cggaacaggt ttgcgtggcg gcggaaattg gcatggaaca caaccttggt ttaacctgcg
     4261 acccggttgc agggcaggtt caggtgccgt gcattgagcg taatgccatt gcctctgtga
     4321 aggcgattaa cgccgcacgg atggctctgc gccgcaccag tgcaccgcgc gtctcgctgg
     4381 ataaggtcat cgaaacgatg tacgaaaccg gtaaggacat gaacgccaaa taccgcgaaa
     4441 cctcacgcgg tggtctggca atcaaagtcc agtgtgacta atacttctta ctcgcccatc
     4501 tgcaacggat gggcgaattt atacccgctt tctcgtctgc tgtaatattc cccactacac
     4561 ttccactgtt gcgtcaggcg tttgtcgcca tacgcttaca gggtggcccg catgcaaaaa
     4621 gcacaacgga tcattaaaac ctatcgccgt aatcgaatga ttgtttgtac gatttgcgca
     4681 ctcgttacgc tcgcttcgac cctgagcgtg cgatttattt cacagcgtaa cttaaatcaa
     4741 caacgggtag tacaattcgc caatcacgct gtagaggaat tagataaagt actgcttccc
     4801 ctacaggcag gtagcaaagt cttgcttccg ctgattggtc tgccctgctc tgtcgcccat
     4861 ttgccattac gtaaacaggc ggcaaaactc caaactgtgc gatccattgg cctggtgcaa
     4921 gacggcacac tttattgctc cagcattttt ggttatcgca atgtgcccgt cgtggacatt
     4981 ctggctgaac ttcctgcacc gcaaccactt ttacgcctga cgatcgaccg tgccctgatt
     5041 aaaggcagtc cggttttgat tcaatggacg cctgcagcgg gcagtagcaa tgctggggtc
     5101 atagagatga ttaacatcga cttactgacg gcaatgctcc ttgagccaca actgccgcaa
     5161 atcagtagcg ccagcctgac ggtggacaaa cggcatttgc tctatggtaa tgggctggta
     5221 gattcccttc cgcaacctga agacaatgaa aactatcagg tttcttcgca aagctttcct
     5281 tttaccatta acgtaaatgg tccgggggct acggcgctgg catggcacta tcttccaaca
     5341 caattaccgc tggcggtgct gctaagttta ctggtgggct atatcgcctg gctggcgacc
     5401 gcttaccgga tgagcttttc ccgcgaaatt aatctgggcc tggcgcaaca tgagttcgaa
     5461 ttgttctgtc agcctttgct taatgcgcgc agccagcaat gtattggtgt agagattttg
     5521 ctgcgctgga ataatccgcg tcagggctgg atttcaccgg atgtgtttat tcctatcgcg
     5581 gaagaacatc atttaattgt gccactgacc cactatgtga tggcagaaac cattcgtcag
     5641 caccatgttt tcccgatgag tagtcagttt catgttggca ttaacgtcgc acccagccat
     5701 tttcgccgtg gtgtgctgat aaaagatctc aatcagtact ggtttagcgc tcacccgatt
     5761 cagcaactga tcctcgaaat caccgaacgc gatgccttac tggatgttga ttatcggatt
     5821 gcccgcgagc tgcatcgtaa aaacgtcaaa ctggcgattg atgacttcgg caccggcaac
     5881 agctcgtttt cctggcttga aacattacgt cctgacgtgc tgaaaattga taagtcattt
     5941 accgcagcta taggttctga cgcggttaac tcgacggtga ccgatatcat catcgcgctg
     6001 gggcaaagac tgaatattga actggtggcg gagggcgtgg aaacgcaaga acaggcgaag
     6061 tatttgcgcc gtcatggcgt gcatattttg caagggtatt tgtacgcaca gccgatgccg
     6121 ctacgtgatt ttcccaaatg gctggcgggc agccaaccgc cgcccgcccg gcataatgga
     6181 catatcacgc ccattatgtc gttacgttaa cgcttactca tcttcatcgt gcgccggttg
     6241 ctctttaaca atgcgaacca gatcaacacg ataatcattg gcttcaaaga tggtgatatg
     6301 cagtggccct acatcaatca catcgcccac acggggaatg tgaccatttg ccgagatcac
     6361 gaggcccgcg accgtcgcga tatcatcgtc atcggcaagg tgctcaacat caagcgcctg
     6421 ctgcaaggca tgcaaatctg taccgccttt taccagccag ccgttaccat cggtaatgat
     6481 ttccggcgtt tcgtcagcgt ccgggaattc acccgcaatg gcttccagca catccagcgg
     6541 cgtgaccaag ccttgtacca caccaaactc gttggtcacg ataacaaagc tcccgcgagc
     6601 gcgacgcagc acgcccagca ggttgatcgg atcgagggtt tccgggacga taatcgccgg
     6661 agacgccgaa gcaatcgccg ccacatcaac gccctcttcc agcgccacca gcagttcttt
     6721 agcacgtacg ataccgatga tttcatccag ttcaccgcga cataccggga acagactgtg
     6781 cggtgaagag agcagttgct cgcggatttc atcgaccccg agattagcgt caacccagct
     6841 tatttcaccg cgcggcgtca tgatcccgcg cagagaacgc gacgccagcg tcagtacgcc
     6901 gttaatcatg taccgttctt cttcggcaaa tgcaccttcc gggatcggca tcgggttatc
     6961 ggcatcgtgc tgaacattgg cctgacgttt cccgcccatc aaacgcagga tggcatcggc
     7021 agtacgcgct cgcagcggca aagtcgactg gtgacgaata aagttgcgac gcgcaatctg
     7081 gttaaacact tcgataatga tcgagaagcc aatcgcggca tacaggtaac ctttcggaat
     7141 gtggaaaccg aaaccttctg ccaccagact cagaccaatc attaacagga agctcagaca
     7201 gagcaccacc accgtggggt gctggttaac gaatcgcgtc agcggtttcg atgccagcaa
     7261 cataaccgcc atcgcaatca ctaccgccgc catcatcacc ggcagatggt taaccatccc
     7321 tactgcagta attaccgcat ccaacgagaa gacggcgtca aggatgacaa tctgtgtgac
     7381 gaccacccag aaactggcgt agcctttacc gtggccggaa tcatgatcgc ggttttccag
     7441 ccgttcatgc agttcggttg ttgctttgaa cagcaagaat atccccccga acaacataat
     7501 cagatcgcgt ccggagaagg agaaatccat gacggtaaat agcggtttgg tcagcgtgac
     7561 catccatgaa atcagcgaca gcagccccag acgcataatc agcgccagtg ataaccccag
     7621 caaacgcgct ttatcgcgtt gttttggcgg cagtttgtca gcaagaatgg cgatgaagac
     7681 caggttatcg atacccagca caatttcgag aacaacaagc gtgagtagcc ccgcccaaat
     7741 tgaggggtcc attaagaatt ccatgacaag ctcctgctta aggaatagct attcgacgcc
     7801 agaaataatg caggcgtaac gacaaaatgc aaacgaaagg tgcggcatag agtgccagaa
     7861 aggcaggcgt taaaaggcct gatgctgaaa tgacgtcggt gacgatccat actgcgggct
     7921 actgccctat actccatggt tgttaaacgg gagttaaaca tatcagagac gcctctgatt
     7981 tggcaaagat ttaccttcct ttgcaaacga atgtgacaag gatattttac ctttcgaaat
     8041 ttctgataat cgaaagttaa attacggatc ttcatcacat aaaataattt tttcgatatc
     8101 taaaataaat cgcgaaacgc aggggttttt ggttgtagcc cttatctgaa tcgattcgat
     8161 tgtggacgac gattcaaaaa tacatctggc acgttgaggt gttaacgata ataaaggagg
     8221 tagcaagtga ccattgctat tgttataggc acacatggtt gggctgcaga gcagttgctt
     8281 aaaacggcag aaatgctgtt aggcgagcag gaaaacgtcg gctggatcga tttcgttcca
     8341 ggtgaaaatg ccgaaacgct gattgaaaaa tacaacgctc agttggcaaa actcgacacc
     8401 actaaaggcg tgctgtttct cgttgataca tggggaggca gcccgttcaa tgctgccagc
     8461 cgcattgtcg tcgacaaaga gcattatgaa gtcattgcag gcgttaacat tccaatgctc
     8521 gtggaaacgt taatggcccg tgatgatgac ccgagctttg atgaactggt tgcgctggca
     8581 gtagaaacag gccgtgaagg cgtgaaagca ctgaaagcca aaccggttga aaaagccgcg
     8641 ccagcacccg ctgccgcagc accaaaagcg gctccaactc cggcaaaacc aatggggcca
     8701 aacgactaca tggttattgg ccttgcgcgt atcgacgacc gtctgattca cggtcaggtc
     8761 gccacccgct ggaccaaaga aaccaatgtc tcccgtatta ttgttgttag tgatgaagtg
     8821 gctgcggata ccgttcgtaa gacactgctc acccaggttg cacctccggg cgtaacagca
     8881 cacgtagttg atgttgccaa aatgattcgc gtctacaaca acccgaaata tgctggcgaa
     8941 cgcgtaatgc tgttatttac caacccaaca gatgtagagc gtctcgttga aggcggcgtg
     9001 aaaatcacct ctgttaacgt cggtggtatg gcattccgtc agggtaaaac ccaggtgaat
     9061 aacgcggttt cggttgatga aaaagatatc gaggcgttca aaaaactgaa tgcacgcggt
     9121 attgagctgg aagtccgtaa ggtttccacc gatccgaaac tgaaaatgat ggatctgatc
     9181 agcaaaatcg ataagtaacg tattgtgttg attatcattc agttttcaca cttaagtctt
     9241 acgtaaacag gagaagtaca atggagatta ccactcttca aattgtgctg gtatttatcg
     9301 tagcctgtat cgcaggtatg ggatcaatcc tcgatgaatt tcagtttcac cgtccgctaa
     9361 tcgcgtgtac cctggtgggt atcgttcttg gggatatgaa aaccggtatt attatcggtg
     9421 gtacgctgga aatgatcgcg ctgggctgga tgaacatcgg tgctgcagtt gcgcctgacg
     9481 ccgctctggc ttctatcatt tctaccattc tggttatcgc aggtcatcag agcattggtg
     9541 caggtatcgc actggcaatc cctctggccg ctgcgggcca ggtactgacc atcatcgttc
     9601 gtactattac cgttgctttc cagcacgctg cggataaggc tgctgataac ggcaacctga
     9661 cagcgatttc ctggatccac gtttcttctc tgttcctgca agcaatgcgt gtggctattc
     9721 cggccgtcat cgttgcgctg tctgttggta ccagcgaagt acagaacatg ctgaatgcga
     9781 ttccggaagt ggtgaccaat ggtctgaata tcgccggggg catgatcgtg gtggttggtt
     9841 atgcgatggt tatcaacatg atgcgtgctg gctacctgat gccgttcttc tacctcggct
     9901 tcgtaaccgc agcattcacc aactttaacc tggttgctct gggtgtgatt ggtactgtta
     9961 tggcagtgct ctacatccaa cttagcccga aatacaaccg cgtagccggt gcgcctgctc
    10021 aggcagctgg taacaacgat ctcgataacg aactggacta acaggtgagc gaaatggttg
    10081 atacaactca aactaccacc gagaaaaaac tcactcaaag tgatattcgt ggcgtattcc
    10141 tgcgttctaa cctcttccag ggttcatgga acttcgaacg tatgcaggca ctgggtttct
    10201 gcttctctat ggtaccggca attcgtcgcc tctaccctga gaacaacgaa gctcgtaaac
    10261 aagctattcg ccgtcacctg gagttcttta acacccagcc gttcgtggct gcaccgattc
    10321 tcggcgtaac cctggcgctg gaagaacagc gtgctaatgg cgcagagatc gatgacggtg
    10381 ctatcaacgg tatcaaagtc ggtttgatgg ggccgctggc tggtgtaggc gacccgatct
    10441 tctggggaac cgtacgtccg gtatttgcag cactgggtgc cggtatcgcg atgagcggca
    10501 gcctgttagg tccgctgctg ttcttcatcc tgtttaacct ggtgcgtctg gcaacccgtt
    10561 actacggcgt agcgtatggt tactccaaag gtatcgatat cgttaaagat atgggtggtg
    10621 gcttcctgca aaaactgacg gaaggggcgt ctatcctcgg cctgtttgtc atgggggcat
    10681 tggttaacaa gtggacacat gtcaacatcc cgctggtggt ttcgaccatc accgggcagg
    10741 atggacagac ccgtgtcacc accgtgcaaa ctattcttga ccagttaatg ccaggccttg
    10801 taccactgct gctgacattt gcttgtatgt ggctgctgcg caaaaaagtt aacccgctgt
    10861 ggatcatcgt tggcttcttc gtcatcggta tcgctggtta cgcttgcggc ctgctgggac
    10921 tgtaagactg tcgtacacta ccggggcctt ttggccccgt ttttttatct ggaggattaa
    10981 tgacaatcac ggacctggta ctgattcttt tcatcgccgc actcctggcc ttcgcgatct
    11041 acgatcagtt catcatgccc cgccgtaacg gccccaccct gctggcaatt cctttgctcc
    11101 ggcgtggtcg catcgatagc gttatcttcg tcggattgat tgtcattctt atctataaca
    11161 acgtcacgaa tcatggcgcg ctaataacaa catggttatt aagtgcactg gctctgatgg
    11221 gtttttatat attctggatc cgcattccga agatcatctt taaacaaaaa ggttttttct
    11281 tcgccaatgt ctggattgaa tatagccgaa tcaaagcgat gaacttgtcg gaagatggcg
    11341 tgttggtgat gcaattagaa cagcgtcggc tgttaatccg cgttcgaaat atcgacgatc
    11401 tggaaaaaat ttataagctt ctcgt
//
LOCUS       DAZXUK010000118        11350 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZXUK010000118 DAZXUK010000000
VERSION     DAZXUK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11350
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.118"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            340..921
                     /gene="mdaB"
                     /locus_tag="ACH3L8_002453"
     CDS             340..921
                     /gene="mdaB"
                     /locus_tag="ACH3L8_002453"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFT4085529.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            952..1266
                     /locus_tag="ACH3L8_002454"
     CDS             952..1266
                     /locus_tag="ACH3L8_002454"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFT4085530.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1314..3206)
                     /gene="parE"
                     /locus_tag="ACH3L8_002455"
     CDS             complement(1314..3206)
                     /gene="parE"
                     /locus_tag="ACH3L8_002455"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFT4085531.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3235..3816)
                     /gene="yqiA"
                     /locus_tag="ACH3L8_002456"
     CDS             complement(3235..3816)
                     /gene="yqiA"
                     /locus_tag="ACH3L8_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFT4085532.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3816..4643)
                     /gene="cpdA"
                     /locus_tag="ACH3L8_002457"
     CDS             complement(3816..4643)
                     /gene="cpdA"
                     /locus_tag="ACH3L8_002457"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFT4085533.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4668..5090)
                     /locus_tag="ACH3L8_002458"
     CDS             complement(4668..5090)
                     /locus_tag="ACH3L8_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFT4085534.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5091..5720)
                     /gene="nudF"
                     /locus_tag="ACH3L8_002459"
     CDS             complement(5091..5720)
                     /gene="nudF"
                     /locus_tag="ACH3L8_002459"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFT4085535.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5925..7406
                     /gene="tolC"
                     /locus_tag="ACH3L8_002460"
     CDS             5925..7406
                     /gene="tolC"
                     /locus_tag="ACH3L8_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFT4085536.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7554..8225
                     /locus_tag="ACH3L8_002461"
     CDS             7554..8225
                     /locus_tag="ACH3L8_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFT4085537.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8231..9391
                     /locus_tag="ACH3L8_002462"
     CDS             8231..9391
                     /locus_tag="ACH3L8_002462"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFT4085538.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9429..10217)
                     /gene="ygiD"
                     /locus_tag="ACH3L8_002463"
     CDS             complement(9429..10217)
                     /gene="ygiD"
                     /locus_tag="ACH3L8_002463"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFT4085539.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     LNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10360..11133
                     /gene="zupT"
                     /locus_tag="ACH3L8_002464"
     CDS             10360..11133
                     /gene="zupT"
                     /locus_tag="ACH3L8_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFT4085540.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11191..>11350)
                     /gene="yqiD"
                     /locus_tag="ACH3L8_002465"
     CDS             complement(11191..>11350)
                     /gene="yqiD"
                     /locus_tag="ACH3L8_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFT4085541.1"
                     /translation="WYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYLNSTR
                     EETAEKVE"
BASE COUNT         2820 a         3062 c         2800 g         2668 t
ORIGIN      
        1 tttaattttc tgctttaaca tatatcagta cgctcataaa aatccattta taattatcaa
       61 catgacacgt tgatattttc atcttttact tattcaaagc catattttca atccccaaaa
      121 aaaccgaacc aaacgtgtaa attattccgg tgcgagtaag aattatgagg aatggctatc
      181 agtattgtca ttttcagaaa atatttatcc tgcatcggtg agtcagagct agatcagact
      241 tttgctaaat tcgcaaaaga ctttgcacat tttgctaatt tcacagtacc gctctgtgac
      301 gtactatagt cgacaaacgt ctcaccttga gattaaaaaa tgagcaacat cctgattatc
      361 aacggcgcga aaaaattcgc ccactccaat ggtcaactga acgacaccct gaccgaagtc
      421 gcggatggca cactgcgcga cctcgggcat gatgtccgca tcgttcgcgc cgacagcgac
      481 tacaatgtca aagcggaagt acaaaacttt ctctgggctg atgtggtgat ctggcagatg
      541 ccaggctggt ggatgggcgc gccgtggaca gtgaaaaaat atattgatga tgtattcacc
      601 gaaggtcacg ggactctgta tgccagcgat ggtcgtaccc gcaaagatcc gtcgaaaaaa
      661 tacggttccg gcagcctggt acaggacaaa aaatatatgc tttctctaac ctggaacgca
      721 ccaatggaag ccttcaccga aaaagatcag ttcttccacg gcgttggcgt tgacggtgtg
      781 tatctgccgt tccataaagc aaaccaattc ctcggtatgg aaccgctgcc gacatttatc
      841 gctaatgacg tgataaaaat gcctgatgtt ccccgctata ctgaagaata tcgcaagcat
      901 cttgtggaaa tttttggtta actagagctc aggctttaga aggagttaac catgcttacc
      961 gtaatcgcag aaatccgtac tcgtcctggt caacatcacc gtcaggcggt attggatcag
     1021 tttgctaaaa tcgttccaac cgtactgaaa gaagaaggtt gccacggcta tgcgccaatg
     1081 gtggattgcg ctgctggcgt gagtttccag tctatggcac cggattctat cgtgatgatt
     1141 gagcagtggg aaagcatcgc gcaccttgaa gcgcatctgc aaaccccgca catgaaggcg
     1201 tacagcgaag ccgtaaaagg cgacgtgctg gagatgaata tccgtattct gcagccaggg
     1261 atttaatcct gccttgtttg cccggccatc ctgaccgggc aatgttcttt cctttaaacc
     1321 tcaatctccg ccatgtcgcc tttctcttgc aaccagttgc ggcgatcttc cgagcgtttc
     1381 ttcgccagca gcatatccat catcgcgtca gtacgctgat cgtcttcatc atcgatagtc
     1441 aactgcacca gacggcgagt gttcggatca agcgtggttt cacgcaattg catcgggttc
     1501 atttccccca gacctttaaa acgctggacg ttcggcttgc ctttcttgcg ttttaattgc
     1561 tcaagtacgc cctctttctc ttcttccgtc agcgcgtaat aaacctcttt cccgagatca
     1621 atacggtaga gcggtggcag tgcgacgtaa acgtgaccgt gtttcaccaa cgcgcggaag
     1681 tgttttacga acaaagcgca gagcagcgtg gcaatgtgca gaccatcaga gtccgcatcc
     1741 gcgaggatac agattttgcc ataacgaagc tggctcagat cgtcgctgtc aggatcgata
     1801 ccgatcgcta ccgaaatatc atgcacttcc tgcgaggcca gcacttcgtc ggaagaaact
     1861 tcccaggtgt taaggatctt acctttcagc ggcatgatcg cctgatattc acgatcgcgc
     1921 gcctgcttgg cagatccgcc tgcggagtca ccttccacga ggaacagttc ggtacggtta
     1981 aggccctgcg cggtacaatc agccagtttg ccaggcagcg ccgggccgct ggtcagcttt
     2041 ttacgcacca cttttttagc cgcacgcata cgacgctggg cgctggaaat cgccatctct
     2101 gccagcaact ccgccgcctg aacgttctgg ttcagccaca ggatgaaggc atctttcacc
     2161 acgcccgaaa cgaatgccgc acactgacgc gaagagagac gctctttggt ctgcccggca
     2221 aactgcggat cctgcatttt aaccgacaga acataggcgc agcgatccca gatatcttcc
     2281 gccgacagct tcacgccgcg cggcagaatg ttgcggtatt cacagaactc acgcatcgcg
     2341 tccaacaggc cctgacgcag accattaaca tgggtaccgc cctgcatcgt tgggataagg
     2401 ttgacgtagc tttcggtcag cagttcaccg ccttccggca gccacagtag cgcccagtcc
     2461 acagcttcag tatcgccagc gaaattaccg ataaacggtt tttctggcag cgtcggtaaa
     2521 ccgtttaccg cttccgccag gtaatcattc agaccgtcct gatagcacca gcgctgttcg
     2581 gtgttgttga tctcatcttt aaaggtaatc tcaacgccgg ggcacagtac tgctttggcc
     2641 ttcagcacat gcgtcaggcg tgaaacagaa aaacgcgggc tgtcaaagaa ggtttcatcc
     2701 ggccagaagt gcacgctggt accggtattg cgtttaccgc aagtgccgac aacctgtaaa
     2761 tcctgcacct tttcgccatt ttcaaaggcg atgttataaa cctgaccatc gcggcgcacg
     2821 ttaacttcta cgcgcttcga caaggcgtta actaccgaaa tccccacgcc atgcaggccg
     2881 ccagagaact ggtaattttt gttagagaat ttaccgcctg catgcagacg gcaaagaatc
     2941 agttcaaccg ccggtactcc ttcttccggg tgaatatcca ccggcatccc gcgcccatcg
     3001 tcaatcactt ctaacgactg gtcagcatgt aagattacat ccacgcgttt tgcgtgaccc
     3061 gccagtgctt catcgacact gttatcaata acttcttgcc ccaaatggtt agggcgagtg
     3121 gtatcggtat acatccccgg acggcggcga accggctcaa gcccggtgag tacctcaatg
     3181 gcatcagcgt tataagtttg cgtcatggtt taagttagca attcgagttg atcgtcagag
     3241 atggtgcaga ccaagaaaat cgacgatcgg gttgaaataa tcttcgaagc ccgtgaatgc
     3301 gtggttgccg ccttctatga cagtctggcg gcaggaagca tagtacgcca ccgcctggcg
     3361 gtaatccagc acttcatctc ccgtctgttg cagcagccag atcaaatccg gcgcttccag
     3421 cgggtcaatc tgcatgactt tgagatcgta aatatggcgt gactctagca catattgctg
     3481 cccggtgtag gggttctcgt tctgaccgag atagtccgtc agcaattcaa acgggcgtac
     3541 cgccgggttt accaccactg cgggcagcat aaaacattgc gacaaccagg tggcgtaata
     3601 tccccccagt gacgaaccga caatacccag cgaatcgccg ccatgttcca ggacaatgga
     3661 ttccagcagt tctgccgcgt cggaaggata cggcggcaac tgcggaatga tcatctcaac
     3721 gtcagggtga tgttccgcca gccagttttt taacaagctc gcttttgcag agcgcggcga
     3781 gctgttgaaa ccgtgtaaat aaagaagcgt agacatcagt agccttctga agcggtatca
     3841 ggttggaaac gtgtgtccgc caggcgatgc acctcggtgg tcagcgtgcc atcagcatgt
     3901 aactcgagag tacgccagcc gggcgcgata gtatccagcg taaagttgga acagtgcggc
     3961 ttaaactgca cacaggtcga cggcgttgcc agcaggcggc gaccgttcca gtcgagatcc
     4021 agctcctgat gaatatgacc gcacagcaag tatttgacgt gcggaaactt cgccagcacg
     4081 gtatccagtt cgcccgcgtt acgcagactg tgttgatcga gccaactaca acccgcaggt
     4141 agcggatgat gatgcagcag cagcaacgta tggcgttctg gcgcatcggc cagtttacgt
     4201 tccagccact caagctgaaa ctcgctcagc tcaccgtgcg gcacgccaaa cacctggcta
     4261 tccagcaaca ggatttgcca ttgctcacca ataaacaccc gcttcgccgg ggagataccc
     4321 gcatcctgta acgcgctgta catcgcgggc tggaaatcgt ggttgcccgg cagccagacg
     4381 cagggcgcac gaaaacttgc gatgccttca gcgaaatgct gataggccgc agaggattga
     4441 tcctgcgcta aatcacctgt cgcgacaatc aggtcgaatt cgtgctggtg tggacgaatc
     4501 gcctccagca ccgcctggta gctttcccag gtgtttaccc ctaacagggc ttcgtgcttt
     4561 tgtgcaaaca ggtgagtatc ggtaatttgt aaaatccttc ctctggcctc accagccaga
     4621 ggaagggtta acaggctttc caaatggtgt ccttaggttt cacgacgcta gtaaaccgga
     4681 atcgccatcg ctccatgtgc taaacagtat cgcaaccagt ccgctaaaaa ctggttaatt
     4741 tgatgctttt cgtcgcgttg atgcaacttt ttattaggat aatcataccg cgctttgaag
     4801 cgaaaaatct gctggcttga acacacttca gccaccatcg cgtcatgata cagacgcacc
     4861 gtcattgacg gaaggctcca gtaactgatc gcgggcgcag tctgttctat tgtcaccagg
     4921 gtagtgtatc gggtcgattc cacaatcgtc agccgatatt gtgcgtttgc cacctgatag
     4981 cttacagttt cgccgggtgc gtcattgcgc ggtaacaaac ggcgcaattg tgaaaagttc
     5041 atctcgcaca ggcgcatcat ttcaggaaag tcaggtgtgt aacgcttcat ttatgcccac
     5101 tcatttttta acgcttgatg atgcagctgc agccattgca aagcgatgac cgacgctgcg
     5161 ttgtcgattt tcccctcttc tacccactgg tatgcctgtt cccggcttac cacatgaacg
     5221 cgaatatctt cgttttcatc agccagacca tgaataccgc ttgcggtcgt ggcgtccact
     5281 tcgcccacca taattgacga acgctcactg gtgccccccg ggcttgccag gaaacttaac
     5341 accggtttgg tccgtttgac tatcagtccc gcctcttcaa tcgcttcgcg acgggcaaca
     5401 tcttccacac tttcaccctc ttcaatcatc ccggcaacca tctccagtag ccagggggtt
     5461 tcgctggtgt cgtacgcggc aatccgaatc tgctcaatca gcacaacttc atcacgcact
     5521 gggtcaaagg gtagcaagac tgcggcgtga ccgcgctcaa aaatttcccg ccgtacctca
     5581 tgactcattt gcccgttgaa tagacgatga cgaaatctat aaagatctaa tgaaaaaaag
     5641 ccgcgataaa gtgtttctcg tgcaataatt tctacatcgt ttttgccaaa tgtaacgggc
     5701 aggttgtctg gcttaagcat tgttaatgtc ctagcactaa tagtgaatta aatgtgaatt
     5761 tcagcgacgt ttgactgccg tttgagcagt catatgttaa attgaggcac attaacgccc
     5821 tatggcacgt aacgccaacc ttttgcggta gcggcttctg ctagaatccg caataatttt
     5881 acagtttgat cgcgctaaat actgcttcac cacaaggaat gcaaatgaag aaattgctcc
     5941 ccattcttat cggcctgagc ctttctgggt tcagttcgtt gagccaggcc gagaacctga
     6001 tgcaagttta tcagcaagca cgccttagta acccggaatt gcgtaagtct gccgccgatc
     6061 gtgatgctgc ctttgaaaaa attaatgaag cgcgcagtcc attactgcca cagctaggtt
     6121 taggtgcaga ttacacctat agcaacggct accgcgacgc gaacggcatc aactcgaacg
     6181 cgaccagtgc gtccctgcag ttaactcaat ccatttttga tatgtcgaaa tggcgtgcgt
     6241 taacgctgca ggaaaaagca gcagggattc aggacgtcac atatcagacc gatcagcaaa
     6301 ccttgatcct caacaccgcg accgcttatt tcaacgtgtt gaatgctatt gacgttcttt
     6361 cctatacaca ggcacaaaaa gaagcgatct accgtcaatt agatcaaacc acccaacgtt
     6421 ttaacgtggg cctggtagcg atcaccgacg tgcagaacgc ccgcgcgcag tacgataccg
     6481 tgctggcgaa cgaagtgacc gcacgtaata accttgataa cgcggtagag cagctgcgcc
     6541 agatcaccgg taactactat ccggaactgg cggcgctgaa tgtcgaaaac tttaaaaccg
     6601 acaaaccaca gccggttaac gcgctgctga aagaagccga aaaacgcaac ctgtcgctgt
     6661 tacaggcacg cttgagccag gacctggagc gcgagcaaat tcgccaggcg caggatggtc
     6721 acttaccgac tctggattta acggcttcta ccgggatttc tgacacctct tatagcggtt
     6781 cgaaaacccg tggtgccgct ggtacccagt atgacgatag caatatgggc cagaacaaag
     6841 ttggcctgag cttctcgctg ccgatttatc agggcggaat ggttaactcg caggtgaaac
     6901 aggcacagta caactttgtt ggtgccagtg agcaactgga aagcgcacat cgtagcgtcg
     6961 tgcaaaccgt acgttcctcc ttcaacaaca ttaatgcttc tatcagtagt attaacgcct
     7021 acaaacaagc cgtagtttcc gcgcaaagct cattagacgc gatggaagcg ggctactcgg
     7081 tcggtacgcg taccattgtt gatgtgttgg atgcgaccac cacgctgtac aacgccaagc
     7141 aagagctggc gaatgcgcgt tataactacc tgattaatca gctgaatatt aagtcagccc
     7201 tgggtacgtt gaacgagcag gatttgctgg cactgaacaa tgcgctgagc aaaccggttt
     7261 ccactaatcc ggaaaacgtt gccccgcaaa cgccggaaca gaatgctatt gctgatggtt
     7321 atgcgcctga tagcccggca cccgtcgttc agcaaacatc cgcacgcact accaccagta
     7381 acggtcataa ccctttccgt aactgatgac gacgacgggg cttcggcccc gtctgaacgt
     7441 aaggcaacgt aaagatacgg gttatctgcc gcattcttcc cccttctcgc ttcaatttcg
     7501 accagccatc ctctattctg atgggtattt accactggtc ccggaagaca aaaatgaaac
     7561 ggacaaaatc catacgccac gcatcgttcc gcaaaaactg gagcgcacgc catctgacac
     7621 cagtcgctct cgcggttgcc actgttttta tgctggctgg ctgtgaaaag agtgatgaaa
     7681 cagtgtctct ctatcaaaat gctgacgact gttcagctgc aaacccaggc aaaagcgccg
     7741 aatgtaccac cgcgtacaac aatgcgctga aagaagccga acgtactgcg ccgaaatacg
     7801 ccacccgtga agactgtgtt gctgaatttg gtgaaggtca gtgtcagcaa gcaccagccc
     7861 aggctggcat ggcaccagaa aaccaggcgc aggctcagca atccagcggg agtttctgga
     7921 tgccgctgat ggccggttac atgatggggc gtctgatggg cggcggcgcg ggatttgcac
     7981 agcagccgct gttctcctcg aaaaacccgg ccagtccggc ttacggtaaa tataccgacg
     8041 cgacgggtaa aaactatggc gcagcccagc caggccgcac catgaccgta ccgaagacgg
     8101 caatggcacc aaaaccggcg accaccacta ccgttacccg tggcggtttt ggtgaatctg
     8161 ttgccaaaca aagcactatg cagcgtagcg caaccggcac ctcttctcgt tcaatgggtg
     8221 gctgataccg atggaaagag tcagtattac cgagcgcccg gactggcgtg agaaagccca
     8281 cgaatacggt ttcaattttc acaccatgta cggcgagccg tactggtgtg aagatgctta
     8341 ctacaagttg accctcgccc aggttgaaaa gctggaagaa gtcaccgccg aactgcacca
     8401 gatgtgcctg aaagtggtgg aaaaagtgat cgccagcgat gagctgatga ccaaattccg
     8461 cataccaaaa cacacctgga gttttgtgcg tcaatcatgg ctgactcacc agccatcgct
     8521 ttattcgcgt cttgatctgg cgtgggatgg cactggcgaa cctaaacttc tggaaaataa
     8581 cgccgatacg ccaacgtcac tatacgaggc ggcgttcttt cagtggatct ggctggaaga
     8641 tcagcttaac gccggtaact tgccggaggg cagcgaccag tttaacagtc tgcaagaaaa
     8701 actgatcgat cgcttcgttg agctgcgtga acagtatggc ttccagttgc tgcatctcac
     8761 ctgctgtcgc gacacggtgg aagatcgcgg aaccattcag tatttgcagg actgcgcaac
     8821 cgaagctgaa atcgccaccg agttcctcta catcgacgag ataggtttag gcgaaaaagg
     8881 ccagttcacg gatttacagg atcaggtgat ttctaacctg ttcaagcttt atccgtggga
     8941 atttatgctg cgcgaaatgt tctccaccaa gctggaagat gctggcgtac gctggctgga
     9001 accggcgtgg aagagcatta tctccaacaa ggcacttcta ccgctactgt gggagatgtt
     9061 cccgaatcac ccgaacctgc tgcccgctta ttttgcggaa gatgatcatc cgcaaatgga
     9121 aaaatatgtg gttaaaccga tcttctcccg tgaaggcgca aacgtgtcga tcattgagaa
     9181 cggcaaaacc attgaagcag cggaaggtcc gtatggcgaa gaagggatga ttgttcagca
     9241 attccacccg ttaccgaaat tcggcgacag ctatatgctg attggtagct ggctggtgaa
     9301 cgatcaacct gccggaattg gcattcgtga agaccgtgcg ttgatcaccc aggatatgtc
     9361 tcggttttat ccacatattt ttgttgaata agccacgata ccggatggca ctcgccatcc
     9421 ggtaattgtt agcctatctg caccgacagc atacttaaac tgcccatttc tataccatcg
     9481 accggaatgg taattggctc ctgcccatcc cacacaccta acacatacaa caacggcaga
     9541 tagtgctctg gcgttgggtt caataacgcg ccaccttcat ggtcgagata attcaccaga
     9601 ggatgctgct ctaccggccc ttgccacgtc agattcgctt tcacatactc attaaacgac
     9661 gtcgcccacg gatacggtga actatcaccg tgccacttca ctgtgcgcag gttatgcacc
     9721 acgttaccgc tggcgaccaa cattattcct tcatcacgca gcgccgcgag tttgcgcccc
     9781 atttcgaaat gccaggcggc aggtttgcta ctgtcgatac tcaactgcac catagggata
     9841 tcggcgtcag gatacatctt aatcagcacg ccccacgagc cgtggtcaaa gccccaggct
     9901 tctttatcca gcgtcaccgg gatcggcgct aacagctcaa ccagacgctg tgccagcgca
     9961 ggcgaacccg gagcaggata atgcgtatcg tacagcgcct gcgggaagcc accaaagtca
    10021 tgaatcgtgg gcggcgtctc catcgcggtc actcctgttc cacgggtaaa ccagtgagcc
    10081 gaaaccacca caatcgcttg cgggcgtggc aatgtcatcc ccaacttctg ccagctgcgg
    10141 gtatacaaat tatcttccag cacgttcatc ggactaccgt gacctaaaaa caatgctggc
    10201 atacgtgttg aagacatgat gatatcctta actaaaggtg tcattttgat atcctcacaa
    10261 tacgcttgtt cggcggagta agaacccgga taacaatgat gatgatcatc agttattttg
    10321 acgatctgcc tgaaggtgaa gatttataag gagttgtcga tgtcagtacc tctcattctg
    10381 accatactgg cgggggcagc cacgtttatt ggcgcgtttc tcggcgttct cggacaaaaa
    10441 ccctcgaacc gcttactggc gttttcgcta ggttttgcgg cggggatcat gttgctcatc
    10501 tcattaatgg aaatgcttcc tgccgcacta gcagctgaag gaatgtcgcc ggtgttgggt
    10561 tatggaatgt ttatcttcgg tctgcttggc tactttggcc tggaccgcat gttgccgcat
    10621 gcgcatccgc aggatttaat gcaaaaatcg gtgcagctgt tgccaaaatc gatcaagcgc
    10681 acagccattc tgctcactct cggcatcagt ctgcataact tcccggaagg gattgccacc
    10741 tttgtcacgg cgagcagcaa cctggagctg ggatttggca tcgcactggc cgtcgcgttg
    10801 cacaatatcc ctgaaggtct ggcagtggca ggcccggttt atgcggcaac ggggtctaaa
    10861 cgcaccgcga ttctgtgggc ggggatttct ggactagcag aaattcttgg tggtgtgctg
    10921 gcgtggttaa tcctcggtag catgatttcc ccggtggtca tggcggcaat catggcggcg
    10981 gttgcaggaa ttatggtggc gctctcggtt gatgaattaa tgccgctcgc caaagagatt
    11041 gaccctaata ataaccccag ctatggcgta ctatgtggaa tgtcagtgat gggattcagt
    11101 ttagtgctgc tacaaacggc gggaattggt taaaaacaac atcagaagtt gtccagcaca
    11161 tggacaactt ctgccagctt cacgaaagcc ttattctacc ttttcggctg tctcttctct
    11221 cgtactgttt aagtatttgt tccgcgcttc aagcagtgcg tctctgtcct ggcgaaacgc
    11281 ccgacaataa cgtttcaaat gcaggaaata acccaccaca accagagcaa tcaatacaat
    11341 ccagtaccag
//
LOCUS       DAZXUK010000119        11285 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZXUK010000119 DAZXUK010000000
VERSION     DAZXUK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11285
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.119"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            159..1300
                     /gene="yghO"
                     /locus_tag="ACH3L8_002466"
                     /pseudo
     CDS             159..1300
                     /gene="yghO"
                     /locus_tag="ACH3L8_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
     gene            complement(1424..2194)
                     /gene="glcC"
                     /locus_tag="ACH3L8_002467"
     CDS             complement(1424..2194)
                     /gene="glcC"
                     /locus_tag="ACH3L8_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFT4085542.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            2445..3944
                     /gene="glcD"
                     /locus_tag="ACH3L8_002468"
                     /pseudo
     CDS             2445..3944
                     /gene="glcD"
                     /locus_tag="ACH3L8_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            3944..4996
                     /gene="glcE"
                     /locus_tag="ACH3L8_002469"
     CDS             3944..4996
                     /gene="glcE"
                     /locus_tag="ACH3L8_002469"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFT4085543.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            5007..6226
                     /gene="glcF"
                     /locus_tag="ACH3L8_002470"
                     /pseudo
     CDS             5007..6226
                     /gene="glcF"
                     /locus_tag="ACH3L8_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            6231..6635
                     /locus_tag="ACH3L8_002471"
     CDS             6231..6635
                     /locus_tag="ACH3L8_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFT4085544.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            6657..8828
                     /gene="glcB"
                     /locus_tag="ACH3L8_002472"
     CDS             6657..8828
                     /gene="glcB"
                     /locus_tag="ACH3L8_002472"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFT4085545.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9183..10769
                     /locus_tag="ACH3L8_002473"
     CDS             9183..10769
                     /locus_tag="ACH3L8_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HFT4085546.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
BASE COUNT         2656 a         2787 c         3123 g         2719 t
ORIGIN      
        1 aaggcattga ggcgtttctg tgtagcctct gaccttatca taatcattaa ttaatactat
       61 ttattcgtcg tttgtattca ttgagttgat tattaagatg ccgctcgttg ctgttgcaac
      121 ctgtttttca ccattaatta tttgttatcc acagagatat ggaatgcgat ctgctgatga
      181 ttaaaattga aaaagtaatt aataaaaatg accttaaggc atttatcgct tttccgtcat
      241 cactttatcc cgacgatcca aactggatac cccctttatt cattgaacgc aacgagcatt
      301 tgtctgcgaa aaatccgggg acggaccata tcatctggca ggcgtgggtg gcaaaaaaag
      361 cggggcagat agttgggcgt attaccgcgc aaatagatac cttgcaccgt gagcgttacg
      421 gtgaagatac cggtcatttc ggcatgattg acgccattga tgatccgcag gattttgccg
      481 cgttgtttgg cgcagcggaa gcgtggttga agtcacaagg tgcaagtaag atcagcgggc
      541 ctttcagcct gaatatcaat caggaaagcg gattactgat tgaaggtttt gacacaccac
      601 cctgtgcgat gatgccacac ggcaaaccgt ggtatgccgc gcatattgaa caactgggtt
      661 atcacaaagg cattgattta ctggcgtggt ggatgcagcg aaccgatctc actttctctc
      721 cggcgctaaa aaaactgatg gatcaggtgc gcaaaaaggt gaccattcgc tgcatcaatc
      781 gtcagcggtt tgccgaagag atgcagatcc tgcgtgagat tttcaactct ggctggcagc
      841 acaactgggg atttgtgccg tttaccgaac atgaattcgc gaccatgggc gaccaactta
      901 aatatctggt gccggacgat atgatctaca tcgctgagat tgattctgca ccctgcgcgt
      961 ttattgtggc ttgcctaaca tcaacgaggc gattgccgat ctgaacggat cgctctttcc
     1021 cttcggctgg gcaaaattgc tgtggcgttt gaaagtcagc ggtgtgcgaa ctgcgcgagt
     1081 accgctgatg ggcgtgcgcg acgagtatca gttcagccgc atcggcccgg tgattgcgct
     1141 gttattgatt gaagccttac gcgatccgtt tgcccgccgg aagatcgatg cgctggagat
     1201 gtcatggatc ctcgaaacca atacaggcat gaataacatg ctggaacgca ttggggcgga
     1261 gccgtacaag cgttaccgat tgtatgaaaa gcaaatttaa taccagatcc tgtttagcgc
     1321 aggggcggtc attcgggaga gggtatagat gaacagattt aacaccagat cctgttaagt
     1381 gcaggggcgg tcccctcgcc ttggggatag ggtaaaactc tgctcacatt cccaccttgt
     1441 tcatctccag cggcaccgcc gagcgaatca aatggtggcc ttccagctcg atatcgtgga
     1501 gattcttttt cacggtccgt acatgatcgc gcgctgcgcg ctgagcgacg tgcggcagcc
     1561 gctgcaacac cgcgttgtag atccgcgcat gctggcgatc gatctgcttt ttttgctgtg
     1621 gtcgatggta gagattattt acgctggcaa acactgaatt aaacatcaga tcggtcaatg
     1681 attgcagcgt aaacaccagc acctgattgt gagaagcctg acaaatggcg agatggaaag
     1741 cgtgatccaa ctgcgcatgt tcgatcagcg aaatctcttt gtggttctca ctggcggcga
     1801 gcattttttc ataacagcgg gttatcacaa caaaatcagc ctgcgttccc agcgttgccg
     1861 ccagccttgc cgattcgccc tccagtaatg cgcgaacgtc gagcagatcg tacagcgttc
     1921 gcggctgcgt actgaacaga tggatcagcg ggctggtgtc ctgctcccga ttaagccgtg
     1981 cgacatgaga atcgcgaccc tgcgccgttt caataatccc gcgcccgcgc agcacggtca
     2041 gcccttcacg cagtgcggag cgtgagaagc cgagcttttc acacagtcga cgttccgagg
     2101 gaagcggctg accgaccttc agtacgccgt cgataattaa ccgttcgata ctctctgcaa
     2161 ccacttcgca aatagggcga cgttcatctt tcattcccgg acctcgtgca caggtaggac
     2221 caatttttct gttcttaact cgcaaaacac gcacatcacg caagtgatta tgttacatca
     2281 atttaacatt aagttaacca agacaaggtc acagagctgg aaaaaaaatg gtctgaccgg
     2341 tagctaaaga gatagacgaa aacgaaaagc ccgcttaata actgttcaca gaagcagcgc
     2401 gcaaaaatca gctgccacac aacacaacaa agcgaagcct actcatgagc atcttgtacg
     2461 aagagcgtct tgatggcgct ttacccgatg tcgaccgcac atcggtactg atggcactgc
     2521 gtgagcatgt ccctggactt gagatcctgc ataccgatga ggagatcatt ccttacgagt
     2581 gtgacgggtt gagcgcgtat cgcacgcgtc cattactggt tgttctgcct aagcaaatgg
     2641 aacaggtgac agcgattctg gctgtctgcc atcgcctgcg tgtaccggtg gtgacccgtg
     2701 gtgcaggcac cgggctttct ggtggcgcgc tgccgctgga aaaaggtgtg ttgttggtga
     2761 tggcgcgctt taaagagagc ctcgacatta accccgttgg tcgccgcgcg cgcgtgcagc
     2821 cgggcgtatg taacctggcg atctcctagg ccgttgcgcc gcataatctc tattacgcgc
     2881 cggatccttc ctcacaaatc gcctgttcaa ttggcggcaa tgtggcggaa aatgccggtg
     2941 gcgtccactg cctgaaatat ggtctgaccg tacataacct gctgaaaatt gaagtgcaaa
     3001 cgctggacgg cgaggcgctg acgctgggat cggacgcgct ggattcacct ggttttgacc
     3061 tgctggcgct gttcaccgga tcggaaggta tgctcggcgt gaccaccgaa gtgacggtaa
     3121 aactgctgcc gaagccgccc gtggcgcggg ttctgttagc cagctttgac tcggtagaaa
     3181 aagccggact tgcggttggt gacatcatcg ccaatggcat tatccccggc gggctggaga
     3241 tgatggataa cctgtcgatc cgcgcggcgg aagattttat tcatgccggt tatcccgtcg
     3301 acgccgaagc gattttgtta tgcgaactgg acggcgtgga gtctgacgta caggaagact
     3361 gcgagcgggt taactacatc ttgttgaaag cgggcgcgac tgacgtccgt ttggcacagg
     3421 acgaagcaga gcgcgtacgt ttctgggccg gtcgcaaaaa tgcgttcccg gcggtaggac
     3481 gtatctcccc ggattactac tgcatggatg gcaccatccc gcgtcgcgcc ctgcctggcg
     3541 tactggaagg cattgcccgt ttatcgcagc aatatgattt acgtgttgcc aacgtctttc
     3601 atgccggaga tggcaacatg cacccgttaa tccttttcga tgccaacgaa cctggtgaat
     3661 ttgcccgcgc ggaagagctg ggcgggaaga tcctcgaact ctgcgttgaa gttggcggca
     3721 gcatcagtgg ggaacatggc atcgggcgag aaaaaatcaa tcaaatgtgc gcccagttca
     3781 acagcgatga aatcacgacc ttccatgcgg tcaaggcggc gtttgacccc gatggtttgc
     3841 tgaaccctgg gaaaaacatt cccacgctac accgctgtgc tgaatttggt gccatgcatg
     3901 tgcatcacgg tcatttacct tttcctgaac tggagcgctt ttgatgctac gcgagtgtga
     3961 ttacagtcag gcgctgctgg agcaggtgaa tcaggcaatt agcgataaaa cgccgctggt
     4021 gattcagggc agcaatagca aagccttttt aggtcgccca gtcaccgggc aaacgctgga
     4081 tgtgcgttgt catcgcggca ttgttaatta cgacccgacc gagctggtga taaccgctcg
     4141 tgccggaacg ccgctggtgg cgattgaagc ggcgctggag agcgccgggc aaatgcttcc
     4201 ctgcgagccg ccgcattatg gtgaagaagc cacctggggc gggatggtcg cctgcgggct
     4261 ggcggggccg cgtcgcccgt ggagcggttc ggtccgcgat tttgtcctcg gcacgcgcat
     4321 cattaccggc actggaaaac atctgcgttt tggtggcgaa gtgatgaaaa acgttgccgg
     4381 atacgatctc tcacggttaa tggccggaag ctacggttgt cttggcgtgc tcactgaaat
     4441 ctcaatgaaa gtgttaccgc gaccgcgtgc ctctctgagc ctgcgtcggg aaatcagcct
     4501 gcaagaagcc atgagtgaaa tcgccgagtg gcaactccag ccattaccca ttagtggctt
     4561 atgttacttc gacaatgcgt tgtggatccg ccttgagggc ggcgaaggat cggtaaaagc
     4621 agcgcgtgaa ctgctgggtg gcgaagaggt tgtgggtcag ttctggcagc aattgcgtga
     4681 acaacaactg ccgttcttct cgttaccagg taccttatgg cgcatttcat tacccagcga
     4741 tgcgccgatg atggatttac ccggcgagca actgatcgac tggggcgggg cgttacgctg
     4801 gctgaaatcg acagccgatg acaatcaaat ccatcgcatc gcccgcaacg ctggcggtca
     4861 tgcgacccgc tttagtgccg gagatggtgg ctttgccccg ctatcggctc ctttattccg
     4921 ctatcaccag cagcttaaac agcagctcga cccttgcggc gtgtttaacc ccggtcgcat
     4981 gtacgcggaa ctttgaggag caggctatgc aaacccgatt aaccgaagag atgcggcaga
     5041 acgcgcgctg gaagccgaca gcatcctgtg cgcctgtgtt cactgcggat tttgtaccgc
     5101 aacctgccca acctatcagc ttctgggcga tgaactggac gggccgcgcg ggcgcatcta
     5161 tctgattaaa caggtgctgg aaggcaacga agtcacgctt aaaacacagg agcatctcga
     5221 tcgctgcctc acttgccgca attgtgaaac cacctgtcct tctggtgtgc gctatcacaa
     5281 tttgctggat atcgggcgtg atattgtcga gcagaaagtg aaacgcccac tgccggagcg
     5341 aatgctgcgc gaaggattgc gccaggtagt gccgcgtccg gcggtcttcc gtgcgctgac
     5401 gcaggtaggg ctggtgctgc gaccgttttt accggaacag gtcagagcaa aactgcctgc
     5461 tgaaacggtg aaagctaaac cgcgtccgcc gctgcgccat aagcgtcggg ttttaatgct
     5521 ggaaggctgc gcccagccta cgctttcgcc caacaccaac gcggcaactg cgcgagtgct
     5581 ggatcgtctg gggatcagcg tcatgtcagc taacgaagca ggctgttgtg gcgcggtgga
     5641 ctatcatctt aatgcgcagg agaaagggtt ggcacgggcg cgcaataata ttgatgcctg
     5701 gtggcccgcg attgaagcag gtgccgaggc aattttgcaa accgccagcg gttgcggcgc
     5761 gttcgtcaaa gagtatgggc agatgctgaa aaacgatgcg ttatatgccg ataaagcgcg
     5821 tcaggtcagt gaactggcgg tcgatttagt cgaacttctg cgcgaggaac cgctggaaaa
     5881 actggcaatt cgcggcgata aaaaactggc cttccactgc ccgtgtaccc tacaacatgc
     5941 gcaaaagctg aacggcgaag tggaaaaagt gttacttcgt cttggattta ccttaacgga
     6001 cgttcccgac agccatctgt gctgcggttc agcgggaaca tatgcgttaa cgcatcccga
     6061 tctggcacgc cagctgcggg ataacaaaat gaatgcgctg gaaagcggca aaccggaaat
     6121 gatcgtcacc gccaacattg gttgccagac gcatctggcg agcgccggtc gtacctctgt
     6181 gcgtcactgg attgaaattg tagaacaagc ccttgaaaag gaataaaaaa atgaaaacta
     6241 aagtcattct tagccagcaa atggcgagtg caattattgc cgcaggtcag gaagaggcgc
     6301 agaaaaataa ctggtctgtt tccattgctg ttgccgatga cggcggtcat ctgctggcgt
     6361 taagtcgcat ggacgattgc gcgccgattg cggcttacat ctcccaggag aaagcgcgta
     6421 ccgccgcgct ggggcgtcgt gaaactaagg gctatgaaga gatggtgaac aacggacgta
     6481 ccgcgttcgt gactgcgccg ttactgacgt cgctggaagg cggcgtaccg gttgttgttg
     6541 atgggcaaat tattggtgcc gtgggcgttt ctggtttaac cggagcacag gatgcgcagg
     6601 tcgcgaaagt ggcagcagcg gtgttggcga aataagcgaa aacgaggaga gaaacaatga
     6661 gtcaaaccat aacccagggc cgtttacgca ttgacgccaa ttttaaacgt tttgtggatg
     6721 gagaagtttt gccgggcgtg gaactggacg ctgctgcatt ctggcacaat gttgatgaga
     6781 tcgttcacga tctggcacca gaaaatcgtc agttgctggc agaacgcgat cgcattcagg
     6841 cggcgcttga tgagtggcat cgcagcaatc cggggccggt aaaagataaa gcggcctata
     6901 aatctttcct tcgtgaactg ggctacctgg tgccgcaacc ggagcgcgtg acggtggaaa
     6961 ccacgggcat tgacagcgaa atcaccagtc aggcggggcc acagctggtg gttccggcaa
     7021 tgaacgcccg ctacgcgctg aacgcggcga acgctcgctg gggctcactg tacgatgcgt
     7081 tatacggcag cgacatcatc ccgcaggaag gggcgatggt cagtggctac gatccgcagc
     7141 gcggtgagca ggttatcgcc tgggttcgac gtttcctcga tgaatcacta ccgctggaaa
     7201 acggcagcta tcaggatgtg gtggcgttta aggtggtcga taaacaatta cgcatccagt
     7261 tgaaaaatgg taaagaaacc acgttacgta ctccagcaca gtttgtcggt taccgtggcg
     7321 atgccgctgc gccgacctgc attttgctga aaaataacgg cctgcatatt gaactgcaaa
     7381 ttgatgccaa cgggcggatt ggcaaagacg atccggcgca catcaacgat gttatcgtcg
     7441 aagctgctat cagtaccatt ctcgactgcg aagattcggt cgcggcggtt gatgcggaag
     7501 ataaaatcct gctgtaccgc aatctgctgg gcctgatgca ggggactctg caagagaaaa
     7561 tggagaaaaa cggtcggcaa atcgtacgta aactgaatga cgatcgtcag tacaccgccg
     7621 ccgatggctc tgaaatttct ctgcacggac gctcgttgct gtttatccgc aacgtgggtc
     7681 atttgatgac cattcctgtg atttgggaca gcgaaggcaa tgaaatcccg gaaggtattc
     7741 ttgatggcgt catgactggc gcgattgccc tctatgattt aaaagtgcag aaaaactcgc
     7801 gcactggcag cgtctatatt gtgaaaccga aaatgcacgg tccgcaggaa gtggcgttcg
     7861 ccaacaaact gtttacccgc attgagacaa tgctcggtat ggcaccgaat accctgaaaa
     7921 tgggcattat ggatgaagaa cgccggacct cgctgaactt gcgtagctgt atcgctcagg
     7981 cgcgcaaccg cgtggcgttc atcaataccg gtttcctcga ccgtaccggc gatgaaatgc
     8041 attcggtgat ggaagctggc ccgatgctgc gtaaaaacca gatgaaatcg acgccgtgga
     8101 tcaaagccta cgaacgtaat aacgtgcttt ccggtctgtt ctgtgggctg cgcggtaaag
     8161 cgcaaatcgg taaaggcatg tgggcaatgc cggacctgat ggcagacatg tacagccaga
     8221 agggcgacca actgcgtgcc ggggcaaaca cagcctgggt tccgtcacca accgctgcta
     8281 cgctccatgc gctgcactac caccatacca acgtacagag cgtgcaagcc aacattgccc
     8341 agaccgagtt cagtgctgaa tttgaaccgc tgctggacga tctgctgact attccggtgg
     8401 ctgaaaacgc taactggtcg gcgcaagaga tccaacaaga gctggataac aacgtgcagg
     8461 ggattctggg gtacgtggtg cgttgggtgg agcaggggat tggttgttca aaagtgccgg
     8521 atattcacaa cgtggcgctg atggaagacc gcgcaacgct gcgtatctcc agccagcata
     8581 tcgccaactg gttacgtcac ggtattctga ccaaagaaca ggtgcaggcg tcgctggaga
     8641 atatggcgaa agtggttgat cagcaaaatg ctggcgatcc ggcttatcgt ccgatggcgg
     8701 ggaatttcgc taactcgtgt gcttttaaag ctgccagcga tttaatcttc ctcggcgtga
     8761 aacagccaaa cggttatacc gaaccgttat tacacgcctg gcgtttacgc gaaaaagaaa
     8821 gtcattaata gcgtcaaaaa agccccggca agttttattg tcggggctat gatagtaact
     8881 gcggtagtac atttatcccg gaaataaccg gagcgattat cagggtttaa caatattaaa
     8941 tacccgtagc gcattactag cgggcctaaa acaacaatat ggacatctta acgatactct
     9001 gcgcctgcgt gtgcggaaca gaaaccctta cccgatatac ttgttatgcc agaggtagca
     9061 attgcgttag cggcgtactg ttgctgtaaa gtataacgcc tatattttac agttcagtcc
     9121 tcttttgctt attacaacct taagtatatc aagcatataa agataataag agactgaaca
     9181 atatggttac ctggacccaa atgtatatgc cgatgggagg actggggcta tccgctctgg
     9241 tcgccctgat cccgataata ttcttcttcg ttgcactcgc ggtattacgt ctgaaaggac
     9301 atgttgctgg agcaataacc cttatattat ctatcctgat tgcgatattc gcctttaaaa
     9361 tgccgattga tatggcattt gctgctgcgg gctatggctt tatttatgga ttatggccaa
     9421 ttgcgtggat tattgtcgcg gcggtgttcc tgtataaatt aaccgttgcc agcgggcagt
     9481 tcgatattat ccgcagctcg gttatctcca tcaccgacga tcagcgtttg caggtgttac
     9541 tgattggttt ctcctttggt gcgttgctgg aaggagctgc tggctttggt gcgacggtgg
     9601 cgtttggtgc gttgggcgtg ccgattctgg tcgccggtca ggtaacggga atcgatccgt
     9661 tccacattgg cgcaatggcg ggacgtcagt taccgttcct gtcggttctt gtgccgttct
     9721 ggctggtagc aatgatggac ggctggaaag gggtgaaaga gacgtggcca gcggcgctgg
     9781 ttgctggggg aagcttcgct gtcactcagt tctttacctc taactatatt ggtccggaac
     9841 tgccggatat tacttcggcg ctggtgagta tcgtctcact cgctttattc cttaaagtct
     9901 ggcggccgaa aaataccgaa acggcaatca gcatggggca atccgcaggt gcgatggtgg
     9961 taaataagcc atcttctggc ggtcccgtgc cttcagaata tagtctgggg caaatcattc
    10021 gtgcgtggtc accgttttta atcttaacgg tgctggtcac catctggacc atgaagccgt
    10081 ttaaagcgtt atttgctccg ggcggcgcgt tttattcact ggtgattaat ttccagatcc
    10141 ctcatttgca tcaacaagtg ttgaaagcgg cacccattgt cgcccaacca acgccaatgg
    10201 atgcggtgtt taaattcgac cctctctcgg ctggcggcac cgctattttt attgcggcga
    10261 ttatctctat cttcatcctc ggcgtgggca tcaagaaagg gattggcgtc tttgccgaaa
    10321 tgctaattag tctgaagtgg ccgatattgt cgattggcat ggtgctggca ttcgccttcg
    10381 tcaccaacta ttccggcatg tccaccacgc tggcgctggt actggcaggt acaggcgtga
    10441 tgttcccgtt tttctcaccg tttctcggct ggctgggcgt attccttacc gggtcggaca
    10501 cttcttcaaa cgccctgttt ggttcactgc aatcgaccac ggcgcagcaa atcaacgtct
    10561 ctgacactct gctggtggca gcaaatacca gcggcggcgt aactggcaag atgatctccc
    10621 cgcaatctat cgccgtggcc tgcgccgcga cgggcatggt gggccgagaa tctgaactgt
    10681 tccgctacac cgtgaagcac agtctgattt ttgccagcgt tatcaccctg ctgcaggcgt
    10741 atgtgtttac cgggatgtta gtctcgtaaa tcaataaagc cggatgaagg taaatatcac
    10801 ccggctttcc atttgtctgt cgtttatctc gccagggaac cgtttttacg attggcttct
    10861 ttatacgtag tctccgtttt acgatgcgaa ttgtttaata tttatacaac ttggcttctg
    10921 tgctggttat aaagtaccca ctttcatgag atcaatttta gtatgttatt acattgcttc
    10981 atctgttgct ttcatgacga atggcgtttt ttctcttatt tatatttttc actagaacaa
    11041 tgagtagatc aaaattccca aaactcccaa aaacataaga ttattcgcaa agctgatttt
    11101 gcagaataat gagaactttt aagatttgtc gaggatcaat tttaagcaat ttatctccac
    11161 ttgttacata aaagatgccg ttaggttaat aagttaatct tgctataaca gtgttcgtta
    11221 ctgcctatta ataaagatat ttgggatagg gtaacgtgga tgaggattcg aaatcatccg
    11281 tcttg
//
LOCUS       DAZXUK010000120        11270 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZXUK010000120 DAZXUK010000000
VERSION     DAZXUK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11270
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.120"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="ACH3L8_002474"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="ACH3L8_002474"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFT4085547.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /gene="malI"
                     /locus_tag="ACH3L8_002475"
     CDS             complement(721..1749)
                     /gene="malI"
                     /locus_tag="ACH3L8_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFT4085548.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /gene="malX"
                     /locus_tag="ACH3L8_002476"
     CDS             1924..3516
                     /gene="malX"
                     /locus_tag="ACH3L8_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFT4085549.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="ACH3L8_002477"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="ACH3L8_002477"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFT4085550.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4802..5803
                     /gene="add"
                     /locus_tag="ACH3L8_002478"
     CDS             4802..5803
                     /gene="add"
                     /locus_tag="ACH3L8_002478"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFT4085551.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5839..6879)
                     /locus_tag="ACH3L8_002479"
     CDS             complement(5839..6879)
                     /locus_tag="ACH3L8_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFT4085552.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7122..7247
                     /gene="blr"
                     /locus_tag="ACH3L8_002480"
     CDS             7122..7247
                     /gene="blr"
                     /locus_tag="ACH3L8_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFT4085553.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7520..7735
                     /gene="ydgT"
                     /locus_tag="ACH3L8_002481"
     CDS             7520..7735
                     /gene="ydgT"
                     /locus_tag="ACH3L8_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFT4085554.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7821..8261
                     /locus_tag="ACH3L8_002482"
     CDS             7821..8261
                     /locus_tag="ACH3L8_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFT4085555.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8338..8919
                     /gene="rsxA"
                     /locus_tag="ACH3L8_002483"
     CDS             8338..8919
                     /gene="rsxA"
                     /locus_tag="ACH3L8_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFT4085556.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8919..9497
                     /gene="rsxB"
                     /locus_tag="ACH3L8_002484"
     CDS             8919..9497
                     /gene="rsxB"
                     /locus_tag="ACH3L8_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFT4085557.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9490..>11270
                     /gene="rsxC"
                     /locus_tag="ACH3L8_002485"
     CDS             9490..>11270
                     /gene="rsxC"
                     /locus_tag="ACH3L8_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFT4085558.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAA"
BASE COUNT         2625 a         2904 c         2842 g         2899 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgctattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccacctc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac ccagattact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc
      421 aaatgcctga ccatccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgtgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccattaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctac caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga catcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc aatcgtggat
     4741 ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa agagtgcgac
     4801 catgattgat accaccctgc cattaactga tatccatcgc caccttgatg gcaacattcg
     4861 tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg cacaatccct
     4921 tgaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg tgagctttct
     4981 gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc gccgcgtggc
     5041 atttgaaaac attgaagatg cagcccgtaa cggcctgcgc tatgtcgagc tgcgtttttc
     5101 accaggctac atggcaatgg cacataagct gcctgtagcg ggtgttgtcg aagcggtgat
     5161 cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta tcggcattat
     5221 gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt tagcccaccg
     5281 tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg gaagtctgtt
     5341 cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc atgcaggcga
     5401 agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg agcgtattgg
     5461 acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg agcaacaaat
     5521 tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag agctggctgc
     5581 acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca ctgacgatcc
     5641 cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag ctgctgggtt
     5701 atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt tcctcagcgc
     5761 agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat ggatggtgca
     5821 aattgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg cggattcgat
     5881 gcccagctca atcaactcca ttacctggat tgcctggctt gccggaaccg gattttcacc
     5941 atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc caggcaccgt
     6001 caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc catcacgcat
     6061 atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt cttcctgtgg
     6121 atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc gtgctgactc
     6181 agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg acaagatggc
     6241 gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta ccgtcatgct
     6301 gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta aatcgtacca
     6361 gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg gacggaagcg
     6421 gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga gcaaaccttt
     6481 tagcgtcaag aaatcgctat cccaacggcg gttatggaat acagacagca cacgccccag
     6541 gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa agggtttatc
     6601 aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat gggtatcgtt
     6661 gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct tcggctcaga
     6721 gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc tggagattac
     6781 cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt tgctcgcata
     6841 accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc tcattgaagt
     6901 atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac ctgccagcaa
     6961 taagggatgt tgtttaactt aagtcaaaaa aatagcgaat tttccaacga caaaagctaa
     7021 atatcgcaaa aacctcagta aaaatcttgc tggagctatt attgctaagt aacatttacc
     7081 ccctgaagtt aatggatcaa tcaagagaga tgtgggctgt aatgaatcgt cttattgaat
     7141 taacaggttg gatcgttctt gtcgtttcag tcattcttct tggcgtggcg agtcacattg
     7201 acaactatca gccacctgaa cagagtgctt cggtacaaca caagtaagct ctgcacttgt
     7261 ggagcgacat gctgcccgtc cgggtgcatg ttttcacttg tcggatatta aaccaggaat
     7321 ttattatctt gttcgatgtt gttggtgatt gtcagggata gtaaagcgcg ttactctttt
     7381 cagagcagtt tttcatccct gaatatttgc catcagcgca ttcttattat tgtaataaca
     7441 caagcgttca cttttggtga aattttatca tcactaataa tacctacccc tttggtatta
     7501 attattttat ggacctttta tgactgttca ggactactta ttaaaatttc gcaaaatcag
     7561 ttcactcgaa agtctggaaa aactctacga ccatcttaat tacaccctga cggacgatca
     7621 ggaactgatc aatatgtatc gtgctgccga tcaccgtcgc gcagagctgg tttctggcgg
     7681 gcgtttgttt gacctcggcc aggtaccgaa gtccgtctgg cactatgtcc aataaagaaa
     7741 gtagcgatcg gttataaaag ccttataata acgcccctaa aattagattt gcctgtttgc
     7801 gtcgctattc tggagaacct atgaccacca caacgccaca acgtattgga ggctggttgc
     7861 ttggcccgtt ggcctggctt ttagtcgctc tgttgagtac gacgctggcg ttgttgctgt
     7921 ataccgctgc gttatcttct ccgcaaacat ttcaaacgct cggcggacaa gcactgacga
     7981 cacaaatctt atggggcgtt tcttttatta ccgctatcgc catgtggtat tacacactgt
     8041 ggttgaccat cgctttcttt aaacgtcggc gttgtgttcc taaacactat attatctggc
     8101 tgcttatttc cgtattgctg gcagtaaaag cattcgcttt ttcacccgtg gaagttggca
     8161 tagccgttcg ccagttgctg tttactttgt tggcaaccgc attgatcgtc ccctatttca
     8221 aacgctcgtc gcgggtaaaa gcgacgtttg tgaatccgta ataaccttac agttaacctg
     8281 ttgtcgcctg ctctggatta acggataata ggcggctttt ttatttcagg ccgaaaaatg
     8341 actgactacc tgttactgtt tgtcggaact gtactggtca ataactttgt actggtcaag
     8401 tttctcggtc tctgtccgtt tatgggggtt tccaaaaaac tggaaaccgc gatgggcatg
     8461 gggctggcaa caacgtttgt gatgacgctg acgtctattt gcgcctggct tatcgatacg
     8521 tggattttga tcccacttaa tctgatttac ctgcgcaccc tggcatttat tctggtgatt
     8581 gctgtggtcg tgcagttcac cgagatggtg gtgcgcaaaa ccagcccggt gctttaccgt
     8641 ctgctgggga tttttttgcc gcttatcacc accaactgtg cagtgctcgg cgtggcgttg
     8701 ctgaatatca atctcgggca caatttcttg cagtcggcgc tgtacggttt ttccgccgct
     8761 gtcggtttct cgctggtgat ggtgctcttc gccgccatcc gcgaacgcct tgctgtggct
     8821 gatgtcccgg caccttttcg cggtaatgcc attgcgttaa ttaccgcagg tcttatgtct
     8881 ctggccttta tgggctttag tggtttggtg aagttgtaat gaatgctatc tggattgccg
     8941 ttgccgccgt gagcctgctg ggcctggcgt ttggcgccat tctgggttat gcctcccgcc
     9001 gttttgcggt ggaagacgat ccggtcgttg agaaaattga cgaaatctta ccgcagagcc
     9061 agtgtggtca gtgcggttat cccggctgtc gcccctacgc ggaagccatc agctgtaacg
     9121 gtgaaaaaat caaccgttgc gccccaggtg gcgaagctgt gatgctaaaa attgccgagt
     9181 tgcttaatgt cgagccgcag ccgctggatg gcgaagcgca agagctaacg cctgcgcgga
     9241 tggtggcggt tattgatgaa aataactgta ttggctgcac taaatgtatt caggcttgtc
     9301 cggtagacgc catcgttggc gctacccgtg tcatgcatac ggtaatgagt gatctctgta
     9361 cgggctgcaa tttatgtgtt gatccgtgcc cgacgcactg catctcgttg caaccggtcg
     9421 cagaaacacc tgactcctgg aaatgggatc tgaacaccat tcccgtgcgt atcattcccg
     9481 tggaacacca tgcttaagtt attctctgca ttcagaaaaa ataaaatctg ggatttcaac
     9541 ggcggcatcc atccaccgga gatgaaaacc cagaccaacg gtacccccct gcgccaggta
     9601 ctactggcgc agcattttgt tattccactg aaacagcata ttggcgctga aggtgagttg
     9661 tgcgttagcg tcggcgataa agtattgcgc ggccagccgc ttacccgtgg tcgcggcaaa
     9721 atgctgcctg ttcacgcgcc cacctcgggt accgttacgg ctattgcgcc ccactctacg
     9781 gctcatcctt cagctttagc tgaattaagc gtgattattg atgccgatgg tgaagactgc
     9841 tggatcccgc gcgacggctg ggtcgattat cgctctcgca gtcgcgaaga gttaatcgag
     9901 cgcatacatc agtttggtgt tgccgggctg ggcggtgccg gattcccgac aggcgttaaa
     9961 ttgcagggtg gtggagataa gattgaaacg ttgattatca acgctgctga gtgcgagccg
    10021 tacattaccg ccgatgaccg tttgatgcag gattgcgcgg ctcaggtcgt agagggtatt
    10081 cgcattcttg cgcatattct gcagccacgc gaaattctta tcggcattga agataacaaa
    10141 ccgcaggcga tttccatgct gcgcgcggtg ctggcggact ctaacgatat ttctctgcgg
    10201 gtgattccaa ccaaatatcc ttctggcggt gctaaacaat taacctacat tctgaccggg
    10261 aagcaggttc cacatggcgg gcgttcatcc gatatcggcg tattaatgca aaacgtcggc
    10321 actgcttatg cagtgaaacg tgccgttatt gatggcgagc cgattaccga gcgtgttgta
    10381 accctgactg gcgaagcaat cgctcgcccg ggcaacgtct gggcacggct ggggacgcca
    10441 gtgcgtcatt tattgaatga tgccggattc tgcccctctg ccgatcaaat ggtgattatg
    10501 ggtggcccgc taatgggctt taccttgcca tggctggatg tcccggtcgt aaagattacc
    10561 aactgtctgt tggctccctc tgccaatgaa cttggcgaac cacaggaaga acaaagctgc
    10621 atccggtgta gcgcctgtgc tgacgcctgc cctgtggatc ttttgccgca acagttgtac
    10681 tggttcagca aaggtcagca acacgataaa gctaccacgc ataacattgc cgactgcatt
    10741 gaatgtgggg cttgcgcgtg ggtttgcccg agcaatattc ccttggtgca atatttccgt
    10801 caggaaaaag ctgaaattgc ggctattcgt caggaagaaa agcgcgccgc agaagccaaa
    10861 gcgcgtttcg aagcgcgcca ggctcgtctg gagcgcgaaa aagcgactcg ccttgaacga
    10921 cataagagcg cagccgttca acctgcagcc aaagataaag atgcgattgc tgccgctctg
    10981 gcgcgggtga aagagaaaca ggcccaggct acacagccta ttgtgattaa agcgggcgaa
    11041 cgcccggata acagtgcaat tattgcagaa cgggaagccc gtaaagcgca agccagagcg
    11101 aaacaggcag aactggagca aactaacgac gcagcaaccg ttgctgaacc acgtaaaact
    11161 gccgttgaag cagctatcgc ccgctccaaa gcgcgcaagc tggaacagca acaggctaat
    11221 gcggaaccag aagaacaggt cgatccgcgc aaagccgccg tcgaagccgc
//
LOCUS       DAZXUK010000121        11243 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZXUK010000121 DAZXUK010000000
VERSION     DAZXUK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11243
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.121"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(109..861)
                     /gene="tcyN"
                     /locus_tag="ACH3L8_002486"
     CDS             complement(109..861)
                     /gene="tcyN"
                     /locus_tag="ACH3L8_002486"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFT4085559.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(858..1526)
                     /gene="tcyL"
                     /locus_tag="ACH3L8_002487"
     CDS             complement(858..1526)
                     /gene="tcyL"
                     /locus_tag="ACH3L8_002487"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFT4085560.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1541..2527)
                     /gene="dcyD"
                     /locus_tag="ACH3L8_002488"
     CDS             complement(1541..2527)
                     /gene="dcyD"
                     /locus_tag="ACH3L8_002488"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFT4085561.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2632..3432)
                     /gene="tcyJ"
                     /locus_tag="ACH3L8_002489"
     CDS             complement(2632..3432)
                     /gene="tcyJ"
                     /locus_tag="ACH3L8_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFT4085562.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3520..4071)
                     /gene="fliZ"
                     /locus_tag="ACH3L8_002490"
     CDS             complement(3520..4071)
                     /gene="fliZ"
                     /locus_tag="ACH3L8_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFT4085563.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4117..4836)
                     /gene="fliA"
                     /locus_tag="ACH3L8_002491"
                     /pseudo
     CDS             complement(4117..4836)
                     /gene="fliA"
                     /locus_tag="ACH3L8_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(5001..6653)
                     /gene="fliC"
                     /locus_tag="ACH3L8_002492"
     CDS             complement(5001..6653)
                     /gene="fliC"
                     /locus_tag="ACH3L8_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFT4085564.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            6919..8325
                     /gene="fliD"
                     /locus_tag="ACH3L8_002493"
     CDS             6919..8325
                     /gene="fliD"
                     /locus_tag="ACH3L8_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFT4085565.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8350..8760
                     /gene="fliS"
                     /locus_tag="ACH3L8_002494"
     CDS             8350..8760
                     /gene="fliS"
                     /locus_tag="ACH3L8_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFT4085566.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            8760..9125
                     /gene="fliT"
                     /locus_tag="ACH3L8_002495"
     CDS             8760..9125
                     /gene="fliT"
                     /locus_tag="ACH3L8_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFT4085567.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9203..10690
                     /gene="amyA"
                     /locus_tag="ACH3L8_002496"
     CDS             9203..10690
                     /gene="amyA"
                     /locus_tag="ACH3L8_002496"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFT4085568.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10724..11137)
                     /gene="yedD"
                     /locus_tag="ACH3L8_002497"
     CDS             complement(10724..11137)
                     /gene="yedD"
                     /locus_tag="ACH3L8_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFT4085569.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
BASE COUNT         2799 a         2934 c         2752 g         2758 t
ORIGIN      
        1 cattctctta tgttattaat gagttatgct gatttgttaa gcagttttat cacgcttgaa
       61 atggcgtccc gccccgacag gtgaatcgtc ggggctgatt ttttcttatt attgcagcag
      121 aagcttctcg aggaactggc gggtgcgagg ctgctgggga tcggcaaata acgcttttgc
      181 tgccccctgc tcgactatcc gcccctggtc cataaagatc gcccggtccg caacatcccg
      241 ggcaaagctc atttcgtgtg tcacaatcac catcgtgcgc ttttcctgcg ccagctggcg
      301 gatggtgttc aggacttcgc ccaccagctc tggatccagc gccgacgttg gctcgtcaaa
      361 caaaatcacc tcaggacgca ttgccagtgc acgcgcaatc gcaacacgct gctgttgacc
      421 gccagacaaa cgacgtggat agctggtttc tttaccggcc agcccaactt tcgccagcaa
      481 ctcgcgagcg cgcgccgtgg cctcttcttt cggttcgcct ttgacgatca ccggcccttc
      541 aataatgttt tccagcaccg tacgatgcgg aaacaaatta aagttctgga agacaaaccc
      601 gacgtgctga cgcaactggc gaatcagcga tttttgctga cttaatgaac gtgcagtatc
      661 aatagtgata tcgccaacgg tgatcgttcc cgcttccggt tgttccagca gatttatgct
      721 acgtagcaat gtggttttgc cggaaccact cggaccgata attgccacca cttcgccagg
      781 ctttacctcg aggtcgatac cgtgcagcac cgtctgaccg tggaattttt tcaccaggtt
      841 cttaacttca atggcactca ttttggttct ctctcctggc gattaagttg attctcaaaa
      901 tggttctgca acgtcgataa cacagtcgcc atgatccagt agatgagcga agccgccagg
      961 tacatggtga acacctccag cgtgcgcgag gtaatcaact gcgcctgacg gaacagctcc
     1021 ggcacctgga ttgtcgcggc cagcgacgta tcttttacca gactgataaa gctgttcgac
     1081 agcggcggca acgccacccg cgccgcctgt ggcaaaatcg cgcgacgcat ggtctgccac
     1141 ggcgtcatac caatactggc agcggcttcc cactgacctt tatcaataga agaaatggcg
     1201 gcgcgcagcg tttcggcggc ataggcggca gtgttcagcg acagaccaat cattgctgac
     1261 ggaatgggat ctaattcgat accaaactgc ggcaggccgt aatagatcat aaacagttgt
     1321 gcgatgagcg gcgtaccacg aaaaatagag atataaaaac gcgccagcca gcgcacgggc
     1381 cagattggcg acaggcgcat cagcgcgaga ataaacccga gtagtaagcc aaaaaacatg
     1441 ccgccgatac tgagttgcag ggtataccca gcccctttca acaggaacgg caaggaatca
     1501 ataaccagtt gtatactttc ttgcatgagc gttcccggag ctaaacgtgg ggatgatagg
     1561 cgaacagcgc aggcgcgccg ccggtatgaa taaacagaat cggcccttca tctttgaagc
     1621 gtttctgact gataccgtca atcagccccg ccatcgcttt tccggtatac acaggatcaa
     1681 gcagaatgcc ttccagacgc gccagcaatt tcactgcttc catgccttcg tcgttcggca
     1741 cgccgtagcc aggtgcaaaa tagtcatccc agagtaaaat ttccgctgat gcggtcagct
     1801 ccagttcttt cgcaatcgcc tgttgtaggt taaccacttt cggcaattga tcggcaacgg
     1861 aacgcgacac ggtcacgcca atcagttcgc tttcaggcat caggtgttcc agcccaacag
     1921 ccagtccggc gtgagttccg gcactgcccg atgcgactac caccgacgaa atattaaccg
     1981 ccccttcaca ctgttgcgcg atttccagcg cactctccac ataacctagc gcgccaagag
     2041 cattagaacc gccaaccgga atgacatacg ggcgaaagcc ttgtgcttcg actcgcgtcg
     2101 ccagttcttc cagttgggca ttgggatcgg tcagtgcgtc gcacatttca atctgggtat
     2161 tgaacagatc cagcaacaaa cgattgccgt tggttaaata gttttctgcg gttgtgccaa
     2221 taggattttc cagcagcgcc acgcagtgca gaccgagttt cgccgcgact gcggcagtct
     2281 ggcgcacatg gttagactgg atcgcgccgg cagtaatcag cgtatcggca ccttcgcgca
     2341 gggcatctgc cgcgagaaat tccagcttac gtaatttatt gccgcccatt gccatggggg
     2401 tgacctcatc ccgtttgatg aaaatttccc gtccaagata atcagaaaag cgcggcagat
     2461 attcgagcgg cgttggcgcg ccgataaact ccaaccgtgg aaaacgggtt aaattatgca
     2521 gtggcataac agcctccgat gtgtgttgtt gtgattttct tattatgcac gctgaaaacg
     2581 cgtaaataaa aaaggcgcta gtgaaagcgc ccttttttgt cattatgctg attatttggt
     2641 cacatcagca ccaaaccatt tttcggaaag agcttgcaga gtgccgtctt tttgcatttc
     2701 cgcaattgca tcattcaccg ctttcagcag gtcttcattt cctttacgca gcgccacgcc
     2761 agactcctga cgggagaatg cttcaccggt tactgccagc gtatcgttgg ttttcttcac
     2821 cagatccagc gccgccagac gatcaacgag gatcgcatcg atacgcccta cgcgcagatc
     2881 ctgatatttg gtcgggtcat catcataggt acgcacatcg acaccctgaa cattctgccg
     2941 cagccactct tcatagttgg tgcccagacc gacacccact tttttgcctt tcagatcagc
     3001 agccgtttta atggtgcctt cgttaccttt tttcaccagc gcctgaatac cggaaatggt
     3061 gtaaggggta gagaaatcgt attttttctt gcgctcgtca gaaatggtga cctgattaat
     3121 caccacatca atgcgtttag agtccagcga cgccagcata ccgtcccatt tggtcggttt
     3181 tagtgacgcc tcaacgccaa gatgttttgc cagctgttgg gcaaattcca cttcaaaacc
     3241 ggttaatttg ccgtcatccc cctgaaaact gaacggcgga taagtgcctt ccagccctac
     3301 cagcagcgtg ccgctctctt tgactttatt aagcagacct tcatctgcaa aacttttaac
     3361 gctcatgccc gcaaccagcg ccacggccat cacacccatc aatgcctgac gtcccagatg
     3421 tgctaatttc atattcaccc cgaatgttgt tatgtctgtt tgcagtgtag agccattgtt
     3481 tgtaaacaca aaaacaactc cgctacatct tattcttatt taatatatat cagaagcagg
     3541 caggctggag gatttctgca caagtccggt acaggtttgg cgctgatagt gttgatactt
     3601 ccgtaatgcg atgcggtaat tgttggtgct ggtggtgggc agccacgggg cgaggatttc
     3661 atcaaggaaa ccgtcctgca gctgatcgag ggaaatattt tgctcatgta gatgattacc
     3721 caaacggcgc agacgaacga catattcacg caccgtacct gggctcattt cagtttgctc
     3781 aaaaagaaat ttcttaaagc cgataatatc gaaaaaatca ctctgcgttt tgcaatgtaa
     3841 atcaccgcaa aatcggcaca atgccgccca tgattgttgt tccgtttgcc agcctttttc
     3901 atcaagcagc gtgtccaggc gggaaatctc gattttattc actattttgc cgtcgcgaac
     3961 taaggtaatg cgatcgagta atttacggca atgcgcgcaa tgggtctggc tgtgtttaaa
     4021 gtctttaaga tagcggctta atggccgtct tttcaggtgc tgcaccatca ttaagaactc
     4081 ctggtagtca aagttaaagt gcggcattta ctgacgttat aacttaccca gtttagtgcg
     4141 taaccgttta atggcctggc tgtgtaactg actgacccgc gattccccga cctccagcac
     4201 cgcgccaatc tctttgagat tcagctcttc ctggtaatag agggttaata ccagtttttc
     4261 gcgctccggc aacgtttcga tggcttccat cacccgctgg cgcagattac tgtccagcag
     4321 ttgttgtagc gggttttctc gctgatgatc atcagtaacc agttcgatgc tatcgccgtg
     4381 ctcttcacgc cactcatcgt aggagaagag ctggctgtta ttggtgtcga gcagcatttg
     4441 acgataatcg gcaatatcga tccctaaacg ttccgctacc tcagtttccg tggcgttgcg
     4501 gccaagttcc tgctccagtt gccctattgc ctgtgccact tcacgcgcgt tgcgtcgcac
     4561 gctgcgcggc acccagtcac ggctgcgaag ttcatccagc atagcgccac ggatacgcta
     4621 cactgcgtaa gttgtaaatg ccgttccttg tagggcgtca tagcgttcga cagcattaag
     4681 taacccaatg ccgcccgcct gtagcagatc gtcaagttcc acgctcgcgg gcagtcgaac
     4741 ctgcaggcgc aatgcttcgt gacgcaccag cgggacataa cgctgccaca gcgagtgttt
     4801 atccattaca ccttcagcgg tatagagtga attcacgata aacagccctg cgttatatga
     4861 gttatcggca tgattatccg tttctgcagg gtttttaatc ggacgattag tgggtgaaat
     4921 gaggggttat ttgggggtta cgcaaaaagc cgaatgtaaa aaaccccgcc ggtggcgggg
     4981 tttgagaggc attcagacga ttaaccctgc agcagagaca gaacctgctg cggtacctgg
     5041 ttggcttttg ccagcacgga gttaccggcc tgctggataa tctgcgcttt cgacatgttg
     5101 gacacttcgg tcgcatagtc ggcgtcctga atacgggact gcgcttcaga caggttggta
     5161 gtggtgttgt tcaggttggt gactgcggaa tccaaacggt tttgcaccgc acccagggag
     5221 gagcggaatt tgtcgatgga gctgatggct tcatccagag ctttcagggg atcggctgtc
     5281 gtagctgctt ttgtttcagc atctgtcgtc aatttaccat cagcatcttt ataaactgcc
     5341 ttaccgctgc cgttagtcac tgaaccatca tcctgtaggt aaaaatctac agtatcagat
     5401 gtggtcaacg cctcgttgtt atcaacataa accgaatcaa caccttttgt tacagcgcca
     5461 gttgtgccgt tgacgctata acctgcagta gtgaaatcaa cattgtttcc cgaggttgat
     5521 acagctttat cgaaagctgc ctgatcaact tttgcacctg ttactgaata agtaatagtg
     5581 tccggcgtag tactattacc ggtaaaggag atagaggtac cgccaatgtc aatggttgtc
     5641 gctttggtat tattggctgc aagtgtggac agactagcct cagtcaaaga agcaccagag
     5701 ccgtttttcg ttaagcgccc agttgtatca atgtagagtt tatctccatt gcttgccgta
     5761 attttaccat cactgctgat ctgaacatcc tgtgcagaac catcgatagt aatagtgccc
     5821 ttagcggtat caccaactcc cggggtcaaa tatttctgaa catcggcagc tgaagccgtt
     5881 gtggtatcaa aagagtatga cttacttgca ctgtcatact tgtaattcgt tgcactagcc
     5941 gccgttccga agccgttatt aatggttgcc tgaacagtat caccatcact aactccagcc
     6001 agcaaatcag acgctttagc agcatcgtaa cccgcactca cagtatattt gttgccgacc
     6061 actgttgcat tagcagctac caagtcagct ttagatgcag cagtattagc cacagccccg
     6121 ccaccattca cattaaaccc attcagcccc agcgtatcag agtcaatttt cttcagatca
     6181 atagtgatag tctggccgtc attcgcacca acctgaattt tcatcgaacc gtcttttgcc
     6241 agtacgttca cgccgttgaa ctgggtctga ccggatacgc ggtcaatttc gtcgagacgg
     6301 gatttgattt cgtcctgaat ggagtccaga tccgaatcag agttagtccc ggtagaagcc
     6361 tgaaccgtca gctcacggat acgctgtaag ttgttgttga tttcggacag cgcgccttca
     6421 gtggtctgtg caacagaaat accgtcgttg gcgttacgtg cagcctgagt caggccttta
     6481 atgttagaag taaaacggtt agcaatcgcc tgacccgcgg cgtcatcctt cgcgctgtta
     6541 atacgcaagc cagaagacag acgctcgata gaactcgaca gcgcagactg gttcttgttg
     6601 atattatttt gagtgatcag cgagaggctg ttggtattaa tgacttgtgc catgattcgt
     6661 tatcctatat tgcaagtcgt tgattacgtt ttgggtttcc acccgtcggc tcaatcgccg
     6721 tcaaccctgt tatcgtctgt cgtaaaacaa cctttagaat ttttttcaca aacagccatt
     6781 ttttgttagt cgacgaaata ctcttttctc tgccccttat tcccgctatt aaaaaaaaca
     6841 attaaacgta aactttgcgc aattcaggcc gataaccccg gtattcgttt tacgtgtcga
     6901 aagataaaag gaaatcgcat ggcaagtatt tcatcgctgg gagtcgggtc aggtctggat
     6961 ttaagttcaa tccttgatag cctcaccgcc gcgcaaaaag cgacgctaac ccctatttca
     7021 aatcagcaat cgtcgtttac cgctaaactt agcgcctacg gtacgctgaa aagcgcgctg
     7081 acgactttcc agaccgccaa tactgcattg tctaaagccg atcttttttc cgccaccagc
     7141 accaccagca gcaccaccgc gttcagtgcc accaccgcgg gtaacgccat cgccgggaaa
     7201 tacaccatca gcgtcaccca tctggcgcag gcgcaaaccc tgaccacgcg caccaccaga
     7261 gatgatacga aaacggcgat cgccaccagc gacagcaaac tcaccattca acaaggcgac
     7321 gacaaagatc cgattaccat tgatatcagc gcggctaact catcgttaag cgggatccgt
     7381 gatgccatca acaacgcaaa agcgggcgta agtgcgagca tcattaacgt gggtaacggt
     7441 gaatatcgtc tgtcagtcac atcaaatgac accggccttg ataatgcgat gacactctcg
     7501 gtcagcggtg atgatgcgct acaaagtttt atgggctatg acgccagtgc cagcagcaac
     7561 ggtatggagg tctcggtcgc cgcccagaat gcgcagctga cggtcaacaa cgtcgccatc
     7621 gagaacagca gcaacactat cagcgacgcg ctggaaaaca tcactctgaa cctgaacgat
     7681 gtcaccacgg gcaaccagac gctaaccatc actcaggaca cctccaaagt gcaaacggcg
     7741 attaaagact gggtgaatgc ctataactcg ctaatagata ccttcagcag cctgaccaaa
     7801 tacaccgccg tagatgcggg agctgatagc cagagttcta gcaatggcgc actgctcggc
     7861 gactccacgc tgcggacgat tcagacgcag ttgaagtcga tgctgagtaa taccgtcagt
     7921 tcttccagct ataaaacgct ggcgcagatt ggtatcacga ccgatcccag cgatggcaaa
     7981 ctggaactgg atgccgacaa actcaccgct gcactgaaaa aagatgccag cggcgtaggt
     8041 gcattgattg ttggcgatgg taaaaaaacc ggcatcacaa ccaccatcgg cagcaacctg
     8101 accagttggc tttcgacaac gggcattatt aaagccgcta ccgatggcgt tagtaaaacc
     8161 ctgaataagt taactaaaga ctacaacgcc gccagcgatc gcattgatgc gcaggtcgcg
     8221 cgctacaaag aacaatttac ccaactggac gttttaatga cctcgttaaa cagcaccagc
     8281 agctacttaa cgcagcagtt cgaaaacaac agtaattcca agtaatcatt attcatcggg
     8341 agacaggtca tgtacgcggc aaaaggcacc caggcctatg cacaaattgg cgtcgaaagc
     8401 accgtaatga gcgccagcca acagcagttg gtcaccatgc tatttgatgg agtgctgagc
     8461 gcactggtta gagcgcgact gtttatgcag gacaacaatc agcaaggcaa aggcgtctct
     8521 ttgtcaaaag cgatcaacat cattgagaac ggactgcggg tgagtcttga tgaagagagc
     8581 aaagacgaat taacccaaaa cttaattgct ctttatagct atatggtcag gcgtttgctg
     8641 caagccaatt tacgcaacga tgtctcagca gtcgaagagg tggaagcatt aatgcgtaat
     8701 attgccgatg cctggaaaga gtctttactc tccccttctt cgattcagga cccagtctga
     8761 tgaacaatgc accgcatttg tatttcgcct ggcaacaact cgtcgaaaaa agccagctca
     8821 tgttacgcct ggcaacggaa gaacaatggg acgaactcat cgctagcgaa atggcgtatg
     8881 tgaatgcggt gcaggagatc gcacacctga ctgaagaggt tgccccgtcc actacgatgc
     8941 aggagcagct ccgcccgatg ctgcacctga ttctcgacaa cgaaagcaag gtaaagcagt
     9001 tattacagat tcggatggat gaactggcga aactggtcgg tcagtcatcg gtgcaaaaat
     9061 cggtgttaag tgcctatggc gatcagggcg gctttgtgct ggctccgcag gataacctct
     9121 tttgaatctt aatgagccga tggctcgcga ataatccgat tacggctacg cttctaatat
     9181 tccccttgaa tggagtcgaa gaatgcgtaa tcccacgctg ttacaatgtt ttcactggta
     9241 ttacccggaa ggcggtaagc tctggcctga actggccgag cgcgcagacg gttttaatga
     9301 tattggtatc aatatggtct ggttgccgcc cgcctataaa ggcgcatcgg gcggctattc
     9361 ggtcggctac gactcctatg atttatttga tctaggtgag tttgatcaga aaggcagcat
     9421 ccctactaaa tatggtgata aagcacaact gctggccgcc attgacgccc tgaaacgtaa
     9481 tgacattgcg gtgctgctgg acgtggtagt caaccacaaa atgggcgcgg atgaaaaaga
     9541 agctattcgc gtgcagcgtg taaatgctga tgaccgtacg caaattgacg aagaaatcat
     9601 tgagtgtgaa ggctggacgc gttacacctt ccccgctcgt gccgggcaat actcgcagtt
     9661 tatctgggat ttcaaatgtt ttagcggtat cgaccatatc gaacaccctg atgaagatgg
     9721 catttttaaa attgttaacg actataccgg agaaggctgg aacgatcagg ttgatgatga
     9781 actcggtaat ttcgattatc tgatgggtga gaatatcgat tttcgcaatc atgccgtgac
     9841 ggaagagatt aaatactggg cgcgctgggt gatggaacaa acgcaatgcg acggctttcg
     9901 tcttgatgcg gtcaaacata ttccggcatg gttttataaa gagtggatcg aacacgtaca
     9961 ggaagttgcg ccaaagccgc tgtttattgt ggctgaatgc tggtcgcatg aagtcgataa
    10021 actgcaaacg tatattgatc aggtggaagg caaaaccatg ctgtttgatg cgccgctgca
    10081 gatgaaattc catgaagcat cgcgcatggg tcgcgactac gacatgacgc agatcttcac
    10141 tgggacgctg gtggaagccg atccttttca cgccgtaacc ctcgttgcca accacgacac
    10201 ccaaccgttg caagccctcg aagcaccggt cgaaccgtgg tttaaaccgc tggcgtatgc
    10261 cttaattttg ttgcgggaaa atggcgttcc ttcggtgttc tatccggacc tctacggtgc
    10321 acattacgaa gatgtcggtg gtgacgggca aacctatccg atagatatgc caataatcga
    10381 acagcttgat gagttaattc tcgcccgtca gcgtttcgcc cacggtgtac agacattatt
    10441 tttcgaccat ccgaactgca ttgcctttag ccgcagtggc accgacgaat atcccggctg
    10501 cgtggtggtc atgtcgaacg gagatgatgg cgaaaaaacc attcatctgg gagagaatta
    10561 cggcaataaa acctggcgtg attttctggg gaaccggcaa gagagcgtag tgacagacga
    10621 aaacggcgaa gcgaccttct tttgcaacgg cggcagcgtc agcgtgtggg ttatcgaaga
    10681 ggtgatttaa atccatcccc ggcggcaagc cggggagatt tcattacggc agtggcgtcg
    10741 gtaacggcgc tttatccagc gcggcggcac actctgccgt cgggcgatcc acacgctcca
    10801 tcgtcatacc atcgtactca atggtgttac cgtcacgctc aacctcgtac agctcacgtt
    10861 ttacggtaac gttagtgaga tcatcagaca tcaatgtcaa cttacccggc acggcaatcc
    10921 cccgctgcca ctgacggcaa tccaaggtat cgccctcttt agtgacaatc aggctgccta
    10981 tcgcttccgg actgaccagc gcacgttgcg gccctttggt ctgccagtag cccgccagcc
    11041 agtccggcgc gggggttttc acgacattgt tatagttttc gacttcggca cagcccgcca
    11101 gcaacatgag cgcgcctgca attgcgagtt ttttcatcat tcgtcctgca tgagaagaaa
    11161 agaggattgt ggcattaaag ctctgatgtc gccagccttt ccaggttcag agagaaattt
    11221 gatctaacta ttatttttgt gaa
//
LOCUS       DAZXUK010000122        11158 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZXUK010000122 DAZXUK010000000
VERSION     DAZXUK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.122"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(59..655)
                     /gene="nrfG"
                     /locus_tag="ACH3L8_002498"
     CDS             complement(59..655)
                     /gene="nrfG"
                     /locus_tag="ACH3L8_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFT4085570.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(652..1035)
                     /gene="nrfF"
                     /locus_tag="ACH3L8_002499"
     CDS             complement(652..1035)
                     /gene="nrfF"
                     /locus_tag="ACH3L8_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFT4085571.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(1028..2746)
                     /locus_tag="ACH3L8_002500"
     CDS             complement(1028..2746)
                     /locus_tag="ACH3L8_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFT4085572.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(2766..3722)
                     /gene="nrfD"
                     /locus_tag="ACH3L8_002501"
     CDS             complement(2766..3722)
                     /gene="nrfD"
                     /locus_tag="ACH3L8_002501"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFT4085573.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(3719..4390)
                     /gene="nrfC"
                     /locus_tag="ACH3L8_002502"
     CDS             complement(3719..4390)
                     /gene="nrfC"
                     /locus_tag="ACH3L8_002502"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFT4085574.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(4387..4953)
                     /gene="nrfB"
                     /locus_tag="ACH3L8_002503"
     CDS             complement(4387..4953)
                     /gene="nrfB"
                     /locus_tag="ACH3L8_002503"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFT4085575.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(4998..6434)
                     /gene="nrfA"
                     /locus_tag="ACH3L8_002504"
     CDS             complement(4998..6434)
                     /gene="nrfA"
                     /locus_tag="ACH3L8_002504"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFT4085576.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6827..8785
                     /gene="acs"
                     /locus_tag="ACH3L8_002505"
                     /pseudo
     CDS             6827..8785
                     /gene="acs"
                     /locus_tag="ACH3L8_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            8985..9299
                     /locus_tag="ACH3L8_002506"
     CDS             8985..9299
                     /locus_tag="ACH3L8_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFT4085577.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            9296..10945
                     /gene="actP"
                     /locus_tag="ACH3L8_002507"
     CDS             9296..10945
                     /gene="actP"
                     /locus_tag="ACH3L8_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFT4085578.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT         2607 a         3120 c         2926 g         2505 t
ORIGIN      
        1 tatgttcatt cgattatttt ttatgcagct caatgacata atcttcatca ccagatgatt
       61 aatccgatct ccgctgcaac agttttgcca tattaatcga ctcgaccagc tgtgtccggt
      121 tgatccgggg tgagttgaga tccatcactt tttgccatag ttcgatggct tgcgcgtaat
      181 tcgcctgcat aaacgcatcg gaagccagca gcatcagagc ggtgatttcg ttagagtcca
      241 gcgcgagggc tttgtcgatc attgcgcgag tctgggcggt catatgctgg ctggcctggt
      301 aataaagcac cgtcgccagc gccgcataca gttcagcgtt ctcaccacgt agttgcaacg
      361 cctgacgata cgccagcagc gaattgctgt aatcgttttg ccacagatag tactcgccca
      421 gtaacgccca ttgttcgctg ttttgtggat tagcgcggat tttatcctgc aatgcctgaa
      481 gctgcgcttc cggggtttgc tggctggcaa actgatgtag cggatcgcgc tgacgctgat
      541 actccgcacg taccgcctgc cattttgggc ttaacagata actgccgaca cagaggaata
      601 ccatcaatat cgtgagtgtt gtcagcattt tcaccggtat ttgaggctgt ttcatcgctt
      661 agccctcact cgccagagga tcagtgccat cagcaataac aacaccactg gcagcgccca
      721 caacaccagc gtctgacccg ttaacggtgg gttatagcga acaaaatctc cgtagcgttc
      781 ggtcatccag ccaatgattt cgacttcgtt cttcccctcc gacaccatgc tataaacctg
      841 atggcgcata ctgaccgcca ccggcgcatt ggattcgagt aagttttgat tctggcactg
      901 cggacaacgt aactggctgg caatatttaa cgcctgttgc tgttgttgcg gattggcgaa
      961 ttgccaggtg tctacaacct gagcgcgcgc aaaacaggta aataacagca gtagcgtgag
     1021 aagcccttta ttcatcgcgc ttcttccccc gccatccgct taacaatgcg cccgcaatca
     1081 tcaacaatcc tcccccccag atccagcgca caccgctttg tacatacaaa cgaaaagcgt
     1141 aacgatccgc tctggttttt tcacccatga ccgcatacca gtcatggatg ccgttccagc
     1201 gaattgacgg ttccatcatt tgctgacggc gtgcttcata aaaacgccgc tccggcgtta
     1261 attcaccaat gcgttgctga tggtcaaaca gcgccactat cgctttttcg ctggtgtaat
     1321 tgcctctggc ttgcagatcg agtcgctcaa aacggaaggt gtatcctgcc agcgtcacct
     1381 gctgacccgg ctgtaaattc aggctgatct cctgacgact gacactggaa accacgatcc
     1441 ccgcggcaaa taacagcacg ccagcatgag ctaccagcgc cggaagctgc acgcgtttgc
     1501 cagagacaaa cgtcgccagc acaatcacca ccagcatcaa cagaccaaac ggtaacgtcg
     1561 cgcggttaaa atacggcgcg ccaacggaga ggcgtcccca gcccagcagg ccgtaaatca
     1621 tcggataaag cgttcccacc agcacgatca gtagcactgc gcaaaacagc aacagcgtag
     1681 cgaggattaa catttcccgc gataaccccg aaaaacgcac cgccgggcca ccgtcccgcg
     1741 ctcgccagcc atacagagcc aaagacgcaa ggctaatcag tgcaaacagg ctgaacaacg
     1801 gcacggcgcg gacgttatcc agtgcgaacg catgaaccga aaccagaatg ccagaacgga
     1861 caattaaggt gcccagcagc gacagcatca gagtaactat cgccagcaac agcgaccagt
     1921 ggcggaaaat cccccgctgg cgtgtcaggg acaaactgtg cagcagcgca gtggcagaaa
     1981 gccagggtaa taaagaggcg ttttccaccg gatcccagaa ccaccagccg ccccagccga
     2041 gttcgcagta ggcccaccag gaaccgagga tgatccccgc cgttaatgca ctccagccag
     2101 gtaacgccca gcgccagcaa attcgggcgc tggccgcatc aaactcgccg cgcagtagac
     2161 tcgccagcgc cacgctcgcc gctaccatca aaccgccata gccaaggtaa agcagcggtg
     2221 gatgaaagat aagaccggga tgttgcagca tcggattgag atcgcggcct tcgattgctg
     2281 gtggaaatat ccgcacgaag ggatcggacc acagcaccac aaacagtagc agtgcggcga
     2341 gcatgagaga taaaacggct agcgtcagcg gaaatagcgg atcgctttgc tgccgataat
     2401 gccaggcaaa cagcgcgctc caggcggaaa gcagcagcac ccaaagcagc agcgaacctt
     2461 catgaccgcc ccacaccgcc gccagtttga gttcccacga cagcaggctg tagctatgtt
     2521 gggcgacata aatgaccgag aaatcgctga tgagaaaaca atacgtcagt acgccaaagg
     2581 cgagcagcag gagcgcgaac tgcgccagaa tgccgatgca agtgagtcgc atcatggcag
     2641 gccagcgcaa ccgcactccc gcaaaggccg tcaacggggt caacacgttg accccgagac
     2701 ttaacaacaa cgccagaaaa ccgacttcag gaaggaatac gtccagaaac cacctctttt
     2761 ctggcttacg ccaccgtcaa ctgtccggcg tagagaatga aaaagcgcag catcaacacg
     2821 ccgaccagac tggccccaca cgccgccagc acggcaggaa tgccggaact gcgattgacc
     2881 cacggtttga gcaacattgg cacaatcagc cccagcccgg cgacaccaag ccagaaccac
     2941 cacgtccaga aaccgccacc taatgccgcc accagcgcac gaactttacc gtcatcgccc
     3001 agcgccagac cgacaaaaaa cgccaccagc aggaagattt caccccatac cacggggatt
     3061 tccatccggt gaacaaagtg cgcttccgtg gaatgcgggt tactgcgttg gcgtatcgcc
     3121 atggcgatca gcgccaccgc cgcaccggac gagatgccgg agaagaggag cagcaccggc
     3181 aggctcgggt tattgaggaa cggatacgat ttcagcgccg acagcagaaa gccggtataa
     3241 gcccccagca gcaccgccaa caccagcatc agcgtttcca gtccgcgatg aacgggcgtc
     3301 agcaggctca gaaccttttg cacgatcccc agcttcggca accagcgttg ttgcagggca
     3361 agcaaatcat gttcaaagat tttcgccagc cacagcacca gcaccaccat gtagagctga
     3421 aacagcatca cccccatcga catcaccgag gtaaaactgt agtggaacat cagcttccag
     3481 aaggtccacg gtcttgtcag gtggaagacg aggatcaaca gaccgaggat caccgcgccc
     3541 ggcccgacaa tcagcgtggt gcgcagcaac gtactgtctg caccgcccgc ctgcgggtag
     3601 aagcgacgta acagcacggc cagcgtcacc agaccggcag aaataccaat caaaaacagg
     3661 tagatggcaa tcggccagtc ccacaccagc gattcaaaat gaaatgcgga agtctgcgtc
     3721 attggctcac ctccccgtat ttaaacggta cgcggtacag cttcggtttg gttcccagcg
     3781 ccagcttgta gcggtaagta ggcttctggc gcagcagttg cgaaatctca ctgttaggat
     3841 cgtccagatt gccaaacgtc agcgccttcg tcgggcaagc ttcaacgcac gcgggaagct
     3901 taccggcctg caaattggtc ttacgacaga aatcgcattt gtccgccgtc ttcgtgaccg
     3961 gatggataaa gcgcacgcgg tacggacagg cggcgatgca gtactgacaa ccgacgcaga
     4021 gatcaggatt aacatcgaca atcccgctag cggcatcacg aaaagacgca ccggtcgggc
     4081 agacgtcaac gcacggcgca tgatcgcagt gctggcaaga cttacggaag aagcgatatt
     4141 tcacatcagg aaattcgccc tgcggctcgc tacgaataat cgtcaggcgc gagacgcctt
     4201 ccggcacctt gttcacttcc cgacaagcat ccatacaggc ggtacagccg atgcataacg
     4261 actcgtcatg caccatgccg taacgcaccc cattgatatt caacgttttc gccaccacgc
     4321 gccctgcggt gccgctgacg gctgccagca cgccgacgcc ggtgagaaac tggcgacgag
     4381 accaggtcat ggctgctcct taagcaacgg aacagacgcc gggttaaagt tcggattggt
     4441 gcgctgatcg ctgtggcaat cgacgcaaat cttaatccgt cctttgtcac ttaacgtctg
     4501 catcgtatct tgctgtggat gcagggaatg gcagctggcg cacgccactt tggttacgtg
     4561 gacatcgtgc ggccagaacg ctttttgcaa ctgttccggc agatgacagg acatacagac
     4621 gctgttctgc tctccgacct tatacatcgg ctcgttaaag cgcatcacat ctttcacccc
     4681 ttcgcggtgt tgcggtgatg cttggccgtg acagttggtg caggtgaccg gcaatttgtt
     4741 attcgggttg ataacggaag catgtttgcc atgcatacct tcggtatctg gtttatgaca
     4801 gtccagacag gcggcatccg ggttacgctg ctgggtaact tcgtagcgat caccagatgc
     4861 ctgcaccgca ggggtagcgg taattccgtt caggctccac aacaggcccg acgccagcac
     4921 cccagcagtt aataacgaac gtaatacgct catattcact ccattgtgtt ttccgcccct
     4981 tgcgaggcgg aacggggtta ttggcttaac agaccgtttt tacgtgcctg ctcttcccac
     5041 tgcgggatca ccgttttgat gaagtcctgc ttctcggcct tgatttgttc catgttcagg
     5101 ccaatggcct gctgggcttt ctctttggtc gagatatccg ggatctggat ttcatgggtg
     5161 atacctttgg tcgccagcag gcgcgccagt ttggtgcgcg catccgccgc tttatccatc
     5221 gccgtaccga gcatccgtag accttcttcc ggtgcgtgca tatgaatacc gtgggaagcg
     5281 atcgccagat cccaacgcca ctgggcatga cggatatcgt cctgaatcgg cttcatttcg
     5341 gcttccgtcg cgcctgcatc cagcgccgct ttcgcttcga agtgagcgtg aaccagttga
     5401 tcttcaacct tgattttcag gtcgttaatc gattgcttac gttccgcgac cactttttgc
     5461 aaggcagctt tatcctgggt atggcagttc gcacaagtct gggcgaagtt atcaaacgga
     5521 ttaccaattt tatggtcggt gtagagtttg ccttcggcgt tctgcacttt tggcatatgg
     5581 cagtcgatac aggtcacgtt gtttttaccg tgaatgcccg ctgtccaggt ttcatattcc
     5641 gggtgctgcg ctttcagcat tggcgttttc gacagggagt tagtccagtc agagaaggca
     5701 attttgtcgt aatactgctc catattttcg actttcatgc cgtcatccca cgggaattta
     5761 accgctttgt ttttgccgtc gaagtaatac tccacatggc actgaccgca aaccatcgat
     5821 tgctggtcga aacgtccggc tttctcaaaa ggtttaccaa tggcttccat cgcgcgagcc
     5881 gcatacggac gggaaagggt taactccggt ttgcctttgg cgaactctgg tgaggcggtg
     5941 ttatggcagt cggcacaacc taagttgttg acgatttccg ggccgccgcg cgcccattta
     6001 ccgtggaagt agccatcttc gccgtctttc tggatcagac gcgccacatc cgggctttta
     6061 caactccagc acgccatcgg tagcggacca tcttcagcgt ttttcggcgc accggtacgc
     6121 agggtttcac gcacatcggt cacagcaaag gcatgtccac gaggcttgtt gtaatcgcgc
     6181 gagaagggat accccgccca caggatcacc agccgtggat cttccgccag ggcgtcaaca
     6241 cgctctgact gttccgaggt ggctttccag gagagatatt gatcgtgatg ctgcggggca
     6301 aaggtttcat tcttcgcttc cacagttaca ggttttgcgg gagctgccgt ttgttcagcg
     6361 tgaacagaag tgaaaaagaa aaaaggaatc aataagctga agatacggcg tgcgtttatt
     6421 tttatccttg tcataggggc ttcatccgaa ttgcgccatt gttgcaatgg cggtttttat
     6481 tgtttttcac gacagtaacc gcacctacac tgtcatgaca ttgctcaccc ctatgtgtaa
     6541 caaataacca cactgtgaat gttgtcttta atcaattgta agtgcatgta aaataccact
     6601 ttagagttag tcagtatctt cctctttttc aacagcatgc ataactgcat gttcctcaaa
     6661 gaattaatca acttttgttg gtgaccttca aaaattaccc tgccgtttat ttgcaccatt
     6721 ctacttttgc gtgatctgtc gcccaaatac taaacaaaac tgccaatacc cctacattta
     6781 acgcttatgc cacatattat taacatccta caaggagaac aaaagcatga gccaaattca
     6841 caaacacacc attcctgcca acatcgcaga ccgttgcctg ataaaccctc agcagtacga
     6901 ggcgatgtat caacaatcta ttaacgtacc tgataccttc tggggcgaac agggaaaaat
     6961 tctcgactgg atcaaaccgt accagaaggt gaaaaatacc tcctttgccc ccggtaatgt
     7021 gtccattaaa tggtacgagg acggcacgct gaatctggcg gcaaactgcc ttgaccgcca
     7081 tctgcaagaa aacggcgatc gcaccgccat catctgggaa ggcgacgacg ccagccagag
     7141 caaacatatc agctataaag agctgcaccg cgacgtctgc cgcttcgcca ataccctgct
     7201 cgagctgggc attaaaaaag gtgatgtggt ggcgatttat atgccgatgg tgccggaagc
     7261 cgcggttgcg atgctggcct gcgcccgcat tggcgcgatg cattcggtga ttttcggcgg
     7321 cttctcgccg gaagccgttg ccgggcgcat tattgattcc aactcacgac tggtgatcac
     7381 ttccgacgaa ggcgtgcgcg ccgggcgtag tattccgctg aagaaaaacg ttgatgacgc
     7441 actaaaaaac ccgaacgtca ccagcgtaga gcatgtggtg gtactgaagc gtactggcgg
     7501 aaacatcgac tggcaggaag ggcgcgacct gtggtggcac gacctggttg agcaagccag
     7561 cgatcagcac taggcggaag agatgaacgc cgaagatccg ctgtttattc tctatacctc
     7621 cggttctacc ggaaaaccaa aaggcgtact gcacactacc ggcggttatc tggtgtacgc
     7681 ggcgctgacc tttaaatatg tctttgatta tcatccgggt gatatctact ggtgcaccgc
     7741 cgatgtgggc tgggttaccg gacacagtta tttgctgtac ggcccgctgg cctgcggcgc
     7801 aaccacgctg atgtttgaag gcgtaccgaa ctggccgacg cctgcccgta tggcgcaggt
     7861 ggtggacaag catcaggtca atattctcta taccgcaccc acggcgatcc gcgcgctgat
     7921 ggcggaaggc gataaagcga ttgaaggcac cgaccgttcg tcgctacgca ttctcggttc
     7981 cgtgggcgag ccaattaacc cggaagcgtg ggagtggtac tggaaaaaaa tcggcaacga
     8041 gaaatgtccg gtggtcgata cctggtggca gaccgaaacc ggcggtttca tgatcacccc
     8101 gctgcctggc gctaccgagc tgaaagccgg ttcggcaaca cgtccgttct tcggcgtgca
     8161 accggcgctg gtcgataacg aaggtaaccc gctggaaggg gctaccgaag gtagcctggt
     8221 gatcaccgac tcctggcctg gtcaggcgcg tacgctgttt ggcgatcacg aacgttttga
     8281 gcagacctat ttttccacct tcaaaaatat gtatttcagc ggcgacggcg cgcgtcgcga
     8341 tgaagatggc tattactgga tcaccgggcg tgtggacgac gtgctgaacg tctccggtca
     8401 ccgtctgggg acggcagaga tagagtcggc gctggtggcg catccgaaga ttgccgaagc
     8461 cgccgtagta ggtattccgc acaatattaa aggtcaggcg atctacgcct atgtcacgct
     8521 taatcacggg gaggaaccgt caccagaact gtacgcagaa gtccgcaact gggtgcgtaa
     8581 agagattggc ccgctggcga cgccagacgt gctgcactgg accgactccc tgcctaaaac
     8641 ccgctccggc aaaattatgc gccgtattct gcgcaaaatt gcggcgggcg ataccagcaa
     8701 cctgggcgat acctcgacgc ttgccgatcc tggcgtggtc gagaagctgc ttgaagagaa
     8761 gcaggctatc gcgatgccat cgtaacccac aattgccgga tgcgaatcgg taacggtttg
     8821 taggcctgat aagacgcgac agcgtcgcat caggcattga ttgccggatg cggcgtaaac
     8881 gccttatccg gcctacattc ggcaagggtt acccgagcgt taaccttctc ccataaggga
     8941 gcgggaatta aaacaatccc tacattacct ctggagaatc tgtgatgaat ggtacgattt
     9001 atcagcggat agaagacaat gcgcatttca gggagttagt cgaaaaacgg caacggtttg
     9061 ccaccatcct gtcgattatt atgctggcag tttatatcgg ctttatttta ctgatcgcct
     9121 tcgcgcccgg ctggctgggc acgccgctga atccgaacac cagcgtcaca cgcggtattc
     9181 caattggtgt tggagtgatt gtgatctcct ttgttctcac cggtatctac atctggcggg
     9241 cgaacggcga attcgaccgt cttaataatg aagtcctgca tgaggtacaa gcatcatgaa
     9301 aagagttctg acggcgcttg ccgccacact ccctttcgca gctaacgccg cggatgctat
     9361 tagcggggcc gtagagcgcc agccaacgaa ctggcaggcg attattatgt tcctgatttt
     9421 cgtcgtgttt acgctcggca ttacctactg ggcatcaaaa cgcgtacgtt ctcgtagcga
     9481 ctactacacc gcaggcggca atatcactgg cttccagaac gggctggcga ttgccgggga
     9541 ctatatgtcc gccgcctcat tcttggggat ctccgcgctg gtgtttacct ccggctatga
     9601 cgggctgatc tactcgttgg gcttcctggt gggctggcca atcattctgt ttctgattgc
     9661 cgaacgtctg cgtaacctgg gacgctacac ctctgccgat gtggcctctt atcgtctgaa
     9721 acaagggccg attcgtattc tttcggcctg tggttctctg gtagtggtgg cgctttacct
     9781 tatcgctcag atggtaggcg caggtaaact gatcgagctg ctgtttggcc ttaactatca
     9841 cattgcggtg gtgctggtcg gcgtgctgat gatgatgtac gtcctgttcg gcggcatgct
     9901 ggcgaccacc tgggtacaaa ttatcaaagc tgtgctgttg ctgttcggtg ccagctttat
     9961 ggcctttatg gtgatgaaac acgtcggctt tagcttcaac aatctgttca gcgaagcgat
    10021 ggcggtacac ccgaaaggtg tcgacatcat gaaaccgggc ggactggtga aagatccgat
    10081 ctccgcgctc tctctggggc tgggactgat gtttggtacg gcgggcttgc cgcacattct
    10141 gatgcgcttc tttacagtca gcgatgcccg cgaagcacgt aagagcgtgt tctacgccac
    10201 cggatttatg ggctacttct atattctgac ctttattatc ggcttcggcg cgatcatgct
    10261 tgttggtgcg aatccggaat ataaagacgc ggcgggccat ctgattggtg gtaacaacat
    10321 ggcggccgtt cacctggcga atgcagtggg cggcaaccta ttcctcggtt ttatttcagc
    10381 ggttgctttc gccactatcc tcgcggtggt tgcggatctg acgctggcgg gcgcatcggc
    10441 ggtttcgcat gacttgtacg ctaacgtctt caaaaaaggc gcgaccgaac gtgaagagct
    10501 gcgggtatca aaaatcaccg tactgatcct cggcgtgatt gcgattatcc tcggcgtgct
    10561 gtttgagaat cagaacatcg cctttatggt aggccttgcg tttgccatcg cggcgagctg
    10621 taacttcccg atcattctgc tttccatgta ctggtcgaaa ctgactaccc gtggcgcgat
    10681 gctgggcggc tggctggggc tgattaccgc agtggtgctg atgatcctcg gcccgactat
    10741 ttgggtacag atcctcggtc acgaaaaagc catcttcccg tatgaatacc cggcgctgtt
    10801 ctctatcagc gtggcattcc tcggcatctg gttcttctcg gcaaccgata actcagcgga
    10861 aggggcgcgc gagcgtgaac tgttccgcgc gcagtttatc cgctcccaga ccggctttgg
    10921 cgttgagcaa ggccgcgcac attaatctct ccccttcccc ggtcgcctga ccggggaata
    10981 cgcttcctct ccaacatgca tcaccttttc ccataatatt aaacaaataa actcattaaa
    11041 aaatgagcga tttttgacag tcgtagaaaa tgataatgca gagaatatgc cttttctttc
    11101 ttgttaatta taaggatatt ttatgcgcta taatggttta aataatatgt ttttccct
//
LOCUS       DAZXUK010000123        11064 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZXUK010000123 DAZXUK010000000
VERSION     DAZXUK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11064
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.123"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..958)
                     /locus_tag="ACH3L8_002508"
     CDS             complement(<1..958)
                     /locus_tag="ACH3L8_002508"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFT4085579.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRT"
     gene            1110..3050
                     /gene="csrD"
                     /locus_tag="ACH3L8_002509"
     CDS             1110..3050
                     /gene="csrD"
                     /locus_tag="ACH3L8_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFT4085580.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQILIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            3355..4398
                     /gene="mreB"
                     /locus_tag="ACH3L8_002510"
     CDS             3355..4398
                     /gene="mreB"
                     /locus_tag="ACH3L8_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFT4085581.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            4464..5567
                     /gene="mreC"
                     /locus_tag="ACH3L8_002511"
     CDS             4464..5567
                     /gene="mreC"
                     /locus_tag="ACH3L8_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFT4085582.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            5567..6055
                     /gene="mreD"
                     /locus_tag="ACH3L8_002512"
     CDS             5567..6055
                     /gene="mreD"
                     /locus_tag="ACH3L8_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFT4085583.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            6064..6657
                     /gene="yhdE"
                     /locus_tag="ACH3L8_002513"
     CDS             6064..6657
                     /gene="yhdE"
                     /locus_tag="ACH3L8_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFT4085584.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            6647..8116
                     /gene="rng"
                     /locus_tag="ACH3L8_002514"
     CDS             6647..8116
                     /gene="rng"
                     /locus_tag="ACH3L8_002514"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFT4085585.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            8184..>11064
                     /gene="yhdP"
                     /locus_tag="ACH3L8_002515"
     CDS             8184..>11064
                     /gene="yhdP"
                     /locus_tag="ACH3L8_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFT4085586.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIK"
BASE COUNT         2572 a         2757 c         3098 g         2637 t
ORIGIN      
        1 gcgtgcgacc ctggatctgg ttattaatga tggcctcggc aaagttcggt gcctctgaca
       61 gagatatctc ttttgccgcc tgggtataga atgattccgg taaatcggcg accagtcgct
      121 gccaggcttg tgcgcggcgt tctggtggcg tcattactga atccacccct tgcaaacgga
      181 cattacgcag aataaatggc atgaccgtgg ttggcagagt aaaaccaccc gccagaccac
      241 aggccgccac gcagccgccg taattcattt gcgccagcac ttttgccagc actttgtcgc
      301 caacagtgtc aattgcccca gcccagactt gtttttccag aggacgggat tcggcaaact
      361 catcacgagg gagaataccg ctggcaccta aacttttcag atattcatgg gtactttcgc
      421 gaccggaaac ggcaacgacc tgataaccca acttatgcag cagcgccacg gcggtactgc
      481 cgacgccacc actggcaccc gtcacgacaa tctcaccgtc ctgcgggcga acaccggcat
      541 cttccagcgc catcacacac agcatggcgg taaaaccggc agtaccgata atcattgctt
      601 tacgcgcgtc cagcccttgc ggcatggcaa ccagccagtc acctttcact cgcgcctgct
      661 ccgccagccc accccagtgg ttttcaccaa cgccccagcc agtgagtaac acctcctgac
      721 cggcatgaaa acgcggatct tcgctggtgc gtacagttcc ggcaaaatcg atcccaggaa
      781 tcatcggaaa attacggatg atttttccct taccggtaat cgccagcgca tctttatagt
      841 tcaggctcga ccagtgaaca tcgaccgtga catcgccctc cggcaggcga cgttcgtcca
      901 gagtctgtac tgatgcgaga gttttgccgt cctgctgttc taaaagtaac gcctgcataa
      961 gtggtcctca tgtgcatgat ggattggaaa ataatttctg aagactatac tcgctaatgg
     1021 aaaagcaaag ccgatgaagc gcaataaatt gcgagataaa tctgatttgc tagtatgccc
     1081 gcttcctcac tatcggagtt aacacaagga tgagattaac gacgaaattt tcggcctttg
     1141 ttacgctgct caccgggtta acaatttttg tgactttgct gggctgttcg ctaagtttct
     1201 acaacgccat tcagtataag tttagtcatc gcgttcaggc ggtggcgacg gcgattgata
     1261 cccaccttgt gtcgaatgac ttcagcgcat taaggccaca aattaccgaa ttaatggtgt
     1321 cagcagatat cgttcgtgta gacctgctgc atggtgataa acaggtttat accctggcca
     1381 gaaatggtag ttatcgtccg gttggcacca gcgatctgtt tcgtgaactg agcgttccgt
     1441 tgataaagca tccggggatg tcgttgcgtc tggtttatca ggatccgatg ggcaactatt
     1501 tccattcgtt gatgaccacc gcgccgctca cgggggcgat tggctttatc attcttatgc
     1561 tctttctggc ggtacgctgg ttacaacggc aacttgccgg gcaagaattg ctggaaaccc
     1621 gggctactcg tatcttaaac ggtgagcgtg gctctaatgt gttgggaacc atctatgaat
     1681 ggccgcccag aaccagcagt gcgctggata cgctgcttcg tgaaattcag aacgcacgcg
     1741 aacaacacag ccgtcttgat acgctgatcc gctcttatgc cgcacaggac gtgaaaaccg
     1801 gcctcaataa ccgactcttt ttcgataatc agttagcaac gttactggaa gatcaggaga
     1861 aagtaggtac ccacgggatc gtgatgatga ttcgtctgcc ggatttcaat atgttgagcg
     1921 atacctgggg gcacagccag gttgaagaac agttcttcac tctgacgaat ctgctgtcga
     1981 catttatgat gcgctaccct ggcgcactgc tggcgcgtta ccaccgcagt gattttgctg
     2041 cgctgttacc gcaccggacg ttaaaagagg cagagagcat agccagtcag ttaatcaaag
     2101 ccgtcgatac cttgccgaac aataaaatgc tcgatcgcga cgatatgatc cacattggta
     2161 tctgtgcctg gcgtagtggt caggataccg agcaggtaat ggaacatgca gagtctgcca
     2221 cgcgtaatgc gggattgcag ggtggcaata gctgggctat ttatgatgac tcgttgcctg
     2281 aaaaaggacg cggtaatgtt cgctggcgta cgcttatcga gcaaatgctc agtcgcggcg
     2341 gcccgcgcct ttatcaaaaa ccggcggtta ctcgcgaagg tcaggttcat catcgcgaac
     2401 tcatgtgccg catcttcgat ggtaatgaag aggttagctc ggcggagtat atgccgatgg
     2461 tcttgcagtt tggcttatcg gaagagtatg accgtctgca aatcagccgt cttattccac
     2521 tattgcgtta ctggccagag gaaaatctgg cgattcaggt taccgttgag tcgctgattc
     2581 gtccgcgttt tcagcgttgg ctgcgcgata cgttaatgca atgtgaaaaa tcacaacgaa
     2641 aacgcataat tattgaactt gcagaggccg atgtaggtca acatatcagt cgtttacaac
     2701 ctgttattcg tttagtgaat gctttagggg tacgggtagc cgtcaaccag gctggtttga
     2761 cgctggtaag caccagttgg atcaaagaac ttaatgttga gttactcaag ctccatccgg
     2821 ggctggtcag gaacattgag aagcgaacgg agaaccagct gctggttcaa agcctggtgg
     2881 aagcctgctc cgggaccagc acccacgttt acgccaccgg cgtgcgttcg cgaagcgagt
     2941 ggcagatcct gattcagcgc ggtgttacag gcgggcaagg ggattttttc gcgtcctcac
     3001 agccacttga tactaacgtg aaaaaatatt cacaaagata ctcggtttaa cctgccgttt
     3061 aatccgtttt cacgtagaat aacgcgcgct gcgtctcatg ggagtgtgct tgtctgctcg
     3121 ccagattgtt gcagcacata tgcagatgaa tgaccttacg cggttgcaaa caggcgagga
     3181 atgctgctga tgcattaagc ctttctggac tcaggcagag atttgtaaca aaggaaacga
     3241 actgcactaa ttttcaccgt agcagatgat ttttgcgcct tgtcgctgct gcgtgtggtt
     3301 ggtaaagtaa gcggattttc ttttccgccc cagctttcag gattatccct tagtatgttg
     3361 aaaaaatttc gtggcatgtt ttccaatgac ttgtccattg acctgggtac tgcgaatacc
     3421 ctcatttatg taaaaggaca aggcatcgta ttgaatgagc cttccgtggt ggccattcgt
     3481 caggatcgtg ccggttcacc gaaaagcgta gctgcagtag gtcatgacgc gaagcagatg
     3541 ctgggccgta cgccgggcaa tattgctgcc attcgcccaa tgaaagacgg cgttatcgcc
     3601 gacttcttcg tgactgaaaa aatgctccag cacttcatca aacaagtgca cagcaacagc
     3661 tttatgcgtc caagcccgcg cgttctggtt tgtgtaccgg ttggcgcgac ccaggttgaa
     3721 cgccgcgcaa ttcgtgaatc cgcgcagggc gctggtgccc gtgaagtctt cctgattgaa
     3781 gaaccgatgg ctgccgcaat tggtgctggc ctgccggttt ctgaagcgac cggttctatg
     3841 gtggttgata tcggtggtgg taccactgaa gttgctgtta tctccttgaa cggcgtggtt
     3901 tactcctctt ccgtgcgcat tggtggtgat cgtttcgacg aagctatcat caactatgtg
     3961 cgtcgtaatt acggttctct gatcggtgaa gccaccgcag aacgtatcaa gcacgaaatc
     4021 ggttcggctt acccgggcga tgaagtccgt gaaatcgaag ttcgtggccg taacctggca
     4081 gaaggtgttc cacgcggttt taccctgaac tccaacgaaa tcctcgaagc actgcaggaa
     4141 ccgctgaccg gtattgtgag cgcggtaatg gttgcactgg aacagtgccc gccggaactg
     4201 gcttccgaca tctccgagcg cggcatggtg ctcaccggtg gtggcgcact gctgcgtaac
     4261 cttgaccgtt tgttaatgga agaaaccggc attccagtcg ttgttgctga agacccgctg
     4321 acctgtgtgg cgcgcggtgg cggcaaagcg ctggaaatga tcgacatgca cggcggcgac
     4381 ctgttcagcg aagagtaatc ggatgcaggc aggggaagcg tctgtttacc ctgcctggtc
     4441 tgatacgaga atacgcataa cttatgaagc caatttttag ccgtggcccg tcgctacaga
     4501 ttcgccttat tctggcggtg ctggtggcgc tcggcattat tattgccgac agccgcctgg
     4561 ggacgttcag tcaaatccgt acttatatgg ataccgccgt cagtcctttc tactttgttt
     4621 ccaatgctcc tcgtgaattg ctggatggca tatcgcagac gctggcctcg cgtgaccaac
     4681 tagaacttga aaaccgggcg ttacgtcagg aactgttgct gaaaaacagt gaactgctga
     4741 tgcttggaca atacaaacag gagaacgcgc gtctgcgcga gctgctgggt tccccgctgc
     4801 gtcaggatga gcagaaaatg gtgactcagg ttatctccac ggttaacgat ccttatagcg
     4861 atcaagttgt tatcgataaa ggtagcgtta atggcgttta tgaaggccag ccggtcatca
     4921 gcgacaaagg tgttgttggt caggtggtgg ccgtcgctaa actgaccagt cgcgtgctgc
     4981 tgatttgtga tgcgacccac gcgctgccaa tccaggtgct gcgcaacgat atccgcgtaa
     5041 ttgcagccgg taacggttgt acggatgatt tgcagcttga gcatctgcca gcgaatacgg
     5101 atattcgtgt tggtgatgtg ctggtgacct ccggtctggg cggtcgtttc ccggaaggct
     5161 atccggtcgc ggttgtctct tccgtaaaac tcgataccca gcgcgcttat actgtgatcc
     5221 aggcgcgtcc gactgcagga ctgcaacgtt tgcgttatct gctgctgctg tggggcgcgg
     5281 atcgtaacgg cgctaacccg atgacgccgg aagaggtgca tcgtgttgct aatgaacgtc
     5341 tgatgcagat gatgccgcag gtattgcctt cgccagacgc gatggggcca aagttacctg
     5401 aaccggcaac ggggatcgct cagccgactc cgcagcaacc ggcgacagga aatgcagcta
     5461 ctgcgcctgt tgcgccgaca cagcccgctg ctaatcgctc tccacaaagg gctacgccgc
     5521 cgcaaagtgg tgctcaaccg cctgcgcgtg cgccgggagg gcaatagtgg cgagctatcg
     5581 tagccaggga cgctgggtaa tctggctctc tttcctcatt gcgctgttgc tgcaaatcat
     5641 gccctggccg gataacctga ttgttttccg gccaaactgg gtgttactta tcttgttgta
     5701 ttggatcctg gccttgcccc atcgcgtaaa tgtgggcaca ggttttgtga tgggtgccat
     5761 actggatctg atcagcggct cgatgcttgg cgtacgcgta ttggcgatga gcatcattgc
     5821 ttacctggtg gcgctgaaat accagctttt ccgcaacctc gcattatggc agcaggcgct
     5881 ggtcgtcatg ttgctttcgc tggtggtgga tattattgtt ttctgggcag agtttttagt
     5941 gattaacgtc tctttcagac cggaagtgtt ctggagtagt gtagtcaatg gggtgctctg
     6001 gccgtggatt ttcttgctga tgcgtaaagt ccgtcagcag tttgcagtgc aataaaggtt
     6061 tctatgactt ctctgtattt agcttccggt tctccgcgtc gtcaggagtt acttgcgcaa
     6121 cttggcgtga cctttgaacg tattgttacg ggcattgagg agcagcgtca gccgcaggag
     6181 agcgcgcagc agtatgttgt gcgtctggca cgcgagaaag cacaggcggg tgtcgcgcaa
     6241 acggcacagg atctcccggt gctgggtgcg gatattatcg ttatcctgaa cggagaagtg
     6301 ctcgagaaac cgcgcgacgc agagcatgcg gcgcagatgt tgcgcaaatt atcgggtcag
     6361 acccatcagg tgatgacggc agtggcgttg gccgacagcc agcacattct cgattgcctg
     6421 gtggtcaccg atgtgacttt cagaacgtta acagacgaag acatcgcggg ctatgtcgcc
     6481 agcggtgaac cgttagataa agcaggtgca tacggtattc aggggctggg tggctgtttt
     6541 gtcaggaaga taaatggcag ctatcacgcc gtagtcggct taccgctggt tgaaacgtat
     6601 gaattattaa gtaattttaa cgcactgcgt gagaaaaggg ataaacatga cggctgaatt
     6661 gttagtaaac gtaacgcctt cggaaacgcg agtggcgtat attgatggcg gtattctgca
     6721 ggaaattcat attgaacgtg aggcgcgacg cggaatagta ggcaatatct acaagggtcg
     6781 tgtaagtcgt gtacttccgg gtatgcaggc ggcttttgta gatattgggc tggataaagc
     6841 cgcgtttctt catgcatccg acatcatgcc gcataccgaa tgtgtggcgg gtgaagaaca
     6901 aaagcaattc acggtgcgcg acatctcgga actggttcgt caggggcaag atctgatggt
     6961 gcaggtggtg aaagatccgc ttggcactaa aggtgcgcgc ctgaccaccg atatcacgct
     7021 gccttctcgc tatctggtat ttatgccagg ggcttctcac gttggggttt cccaacgaat
     7081 tgaaagcgaa tcagaacgtg aacgtctgaa aaaagtggtc gcagagtatt gcgacgagca
     7141 gggcgggttt atcatccgca ccgcagcgga aggggttggc gaggctgaac tggcctccga
     7201 tgccgcttat ctgaaacgcg tctggaccaa agttatggag cgtaaaaagc gcccgcagac
     7261 ccgttatcag ctgtacggcg aactggcgct ggcgcagcgt gttctgcgtg attttgccga
     7321 cgccgaactg gaccgcattc gcgttgactc acgcctgact tacgaagcat tgctggagtt
     7381 tacctcggag tacattcccg agatgaccag caagctggag cattacaccg gacgccagcc
     7441 gattttcgat ctctttgatg tcgaaaacga aatccagcga gcgttggaac gcaaagtaga
     7501 actgaaatcc ggtggttatc tgattatcga ccagaccgaa gcgatgacca ccgtggacat
     7561 caataccgga gcgtttgtcg gtcatcgcaa tctggacgac actattttca ataccaatat
     7621 tgaagcgacg caggctatcg ctcgccagtt acggttgcgt aatctgggcg ggattatcat
     7681 tattgatttc atcgatatga ataatgaaga tcaccgccgc cgcgtgctgc attcgctgga
     7741 gcaggcgttg agcaaagacc gggtgaaaac cagcgttaat ggtttttcgg cgctggggct
     7801 ggtggagatg acgcgtaaac gcacccgcga aagcattgag cacgtactgt gtaacgaatg
     7861 cccaacctgc cacggtcgcg gaacggtgaa aaccgtggaa acggtatgct atgaaatcat
     7921 gcgcgagatt gttcgtgtcc accatgctta cgactccgac cgtttcctgg tctatgcttc
     7981 tccggcagta gccgaagcct tgaaaggcga agagtcacac tcgctggcgg aagtggaaat
     8041 tttcgttggc aaacaggtta aagtacaaat tgaaccgctc tataaccagg agcagtttga
     8101 cgtcgtaatg atgtaaacag atgctgggcc gccatccggc aaagggtttt tgagtcacat
     8161 ttttagcaga caaggagtga cgggtgaggc gattgccggg gattttactg cttactggag
     8221 ccgcgctcgt tgtgatcgct gccctgctgg ttagcggcct gcgtattgct ttaccgcatc
     8281 ttaacgcctg gcgtccggaa atcctcaaca aaatagaatc cgcgactggc atgccggtag
     8341 aagccagtca gctctcagcc agctggcaga attttggccc gacgcttgaa gcacacgaca
     8401 tccgtgcaga actaaaagat ggcggcgaat tttcggttaa acgcgttact ctggcgctgg
     8461 atgtctggca gagcctgtta catatgcgct ggcagtttcg cgacctcact ttctggcagc
     8521 tgcgctttcg caccaacact cctatcacca gcggtggtag cgatgacagt ctggaagcca
     8581 gtcacatcag cgatctgttt cttcgtcaat ttgaccattt cgatctccgc gacagtgaag
     8641 tcagttttct gacgccatcc ggtcagcgcg ccgagctggc gatcccacaa ctcacctggc
     8701 tgaacgatcc acgtcgacac cgtgcggaag gcctggtaag cctctccagc cttaccggac
     8761 agcacggcgt gatgcaggtg cgcatggatt tgcgcgatga tgaggggttg ttaagcaatg
     8821 gtcgcgtctg gctacaggcg gatgacatcg acctgaagcc gtggctcggt aaatggatgc
     8881 aggacaatat tgctctggaa acggcacagt tctcccttga aggctggatg acgatcgaca
     8941 aaggcgatgt aaccggcggt gacgtctggc tgaaacaggg cggtgccagc tggttgggcg
     9001 agaagcaaac gcatacgctg tcggtggata atctgaccgc gcatattacg cgtgaaaatc
     9061 cgggctggca gttctctatt cccgatacac ggatcacgat ggacggcaaa ccctggccga
     9121 gcggagcatt gacgctggcc tggataccgg aacaggacgt tggcggcaaa gacaataaac
     9181 gcagtgacga actccggatt cgcgctagta atctggagct ggcaggcctg gagggcatac
     9241 gcccgctggc cgcgaaactt tcacctgcac tgggtgatgt ttggcgctcc acacaaccga
     9301 gcggcaagat taacactctg gcgctggata tcccgcttca ggcggcagac aagacccgtt
     9361 ttcaggcatc gtggagcgat ctggcctgga agcaatggaa attattaccg ggtgcggaac
     9421 acttctccgg gacgctttcc ggcagcgttg aaaatggttt gcttaccgcg tcgatgaagc
     9481 aggcaaagat gccttacgaa acggtattcc gtgcgccact ggaaatcgcc gacggccagg
     9541 caactataag ctggctgaac aatgacaaag gtttccagct ggatgggcgt aatattgacg
     9601 ttaaagccaa agccgtccat gcgcgcggcg gttttcgtta cctgcaacct gctaacgatg
     9661 aaccctggct gggtattctg gctggcatca gtaccgatga tggttcacaa gcctggcgct
     9721 atttcccgga aaacttgatg ggtaaagacc tggttgatta tttaagtggt gcgattcagg
     9781 gcggtgaagc ggataacgcg acgctggttt atggtggcaa tccgcaactc ttcccctata
     9841 aacacaacga aggtcagttt gaagtgctgg tgccgctgcg caacgcgaag tttgccttcc
     9901 agccggactg gcctgcatta actaaccttg atattgaact ggactttatt aacgacggtt
     9961 tatggatgaa aaccgatggc gttaatctgg gcggcgtgcg cgcgagtaat ctcaccgcag
    10021 tgatccctga ctactcaaaa gaaaaactgc tgattgacgc tgacattaaa ggtccgggta
    10081 aagccgttgg cccttacttt gatgagacac cgctgaaaga ttctctgggt gcgaccctgc
    10141 aagaactcca gctcgacggc gatgtgaatg ctcgcttaca tcttgatatc ccgctgaacg
    10201 gcgaactggt aaccgcgaaa ggtgaagtga cgctgcgtaa taacagtctg tttatcaaac
    10261 cactcgacag catcctgaaa aatttgagcg gtaaattcag ctttatcaat ggcgatctgc
    10321 aaagtgaacc actgacagca agctggttta atcagccgtt gaacgtggat ttttccacca
    10381 aagaaggggc aaaagcctac caggttgcgg tgaatctcaa cggtaactgg caaccggcga
    10441 aaaccggcgt tctgcctgaa gcggtgaacg aagcattgag tggcagcgtg gcgtgggatg
    10501 gtaaagtggg cattgatctg ccttatcatg ctggcgcgac ctataacgta gagctgaacg
    10561 gcgatctgaa taatgtgagt agtcacttac cttcaccgtt agccaaacct gcgggtgaac
    10621 cactagcggt aaacgttaag gttgatggca atctcaacag ctttgaatta accggacagg
    10681 ctggtgcgga taaccatttc aatagccgct ggttgcttgg tcaaaagctg acgctcgatc
    10741 gtgctatttg ggcggcagac agtaaaacgc tcccgccgtt gccggaacaa agtggcgttg
    10801 aacttaatat gccgccgatg aatggtgccg agtggctggc cctgttccag aaaggcgctg
    10861 cggagagtgt cggtggtgca gcgagtttcc cacaacacat aacgttacgt acgcctatgt
    10921 tgtcactggg aaatcagcaa tggaataacc tgagtattgt ttcgcaaccg acggcaaatg
    10981 gcaccctggt tgaggcgcaa gggcgtgaaa tcaacgccac gctagcgatg cgtaataacg
    11041 cgccgtggct ggcgaatatc aaat
//
LOCUS       DAZXUK010000124        11047 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZXUK010000124 DAZXUK010000000
VERSION     DAZXUK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11047
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.124"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            24..158
                     /locus_tag="ACH3L8_002516"
     CDS             24..158
                     /locus_tag="ACH3L8_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFT4085587.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTASGKGKWKIITA
                     "
     gene            137..241
                     /gene="speFL"
                     /locus_tag="ACH3L8_002517"
     CDS             137..241
                     /gene="speFL"
                     /locus_tag="ACH3L8_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFT4085588.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            552..2749
                     /gene="speF"
                     /locus_tag="ACH3L8_002518"
                     /pseudo
     CDS             552..2749
                     /gene="speF"
                     /locus_tag="ACH3L8_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2746..4065
                     /gene="potE"
                     /locus_tag="ACH3L8_002519"
     CDS             2746..4065
                     /gene="potE"
                     /locus_tag="ACH3L8_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFT4085589.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMAPAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4121..5761)
                     /gene="pgm"
                     /locus_tag="ACH3L8_002520"
     CDS             complement(4121..5761)
                     /gene="pgm"
                     /locus_tag="ACH3L8_002520"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFT4085590.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLTDI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5787..6332)
                     /gene="seqA"
                     /locus_tag="ACH3L8_002521"
     CDS             complement(5787..6332)
                     /gene="seqA"
                     /locus_tag="ACH3L8_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFT4085591.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6517..7281
                     /gene="ybfF"
                     /locus_tag="ACH3L8_002522"
     CDS             6517..7281
                     /gene="ybfF"
                     /locus_tag="ACH3L8_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFT4085592.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7421..7714
                     /gene="ybfE"
                     /locus_tag="ACH3L8_002523"
     CDS             7421..7714
                     /gene="ybfE"
                     /locus_tag="ACH3L8_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFT4085593.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7854..8384
                     /gene="fldA"
                     /locus_tag="ACH3L8_002524"
     CDS             7854..8384
                     /gene="fldA"
                     /locus_tag="ACH3L8_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFT4085594.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8594..8680
                     /gene="uof"
                     /locus_tag="ACH3L8_002525"
     CDS             8594..8680
                     /gene="uof"
                     /locus_tag="ACH3L8_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFT4085595.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8673..9119
                     /gene="fur"
                     /locus_tag="ACH3L8_002526"
     CDS             8673..9119
                     /gene="fur"
                     /locus_tag="ACH3L8_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFT4085596.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9203..9529)
                     /gene="chiQ"
                     /locus_tag="ACH3L8_002527"
     CDS             complement(9203..9529)
                     /gene="chiQ"
                     /locus_tag="ACH3L8_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFT4085597.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9566..10972)
                     /gene="chiP"
                     /locus_tag="ACH3L8_002528"
     CDS             complement(9566..10972)
                     /gene="chiP"
                     /locus_tag="ACH3L8_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFT4085598.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2674 a         2735 c         2761 g         2877 t
ORIGIN      
        1 taatatggcg atattcataa ttaatgattt attatctggg gtatgatcgt tttttgttga
       61 tcttcttcac agattatagc catttcatgg atagaataac tctaccttca actgacacag
      121 caagcggtaa aggtaaatgg aaaataataa ccgcttaatg cctcatataa ggcggacaac
      181 ccatatcatg aagtttgccc atcgtaatag ttttgacttt catttcttta atgcccgcta
      241 gtcttctgac taaagggcac cccaacgtac aggtctccct gactttaagc attacaggtt
      301 aatacctgta ttcctcggtg ctcatatact gctaaccctt ttaaactcta aataattcga
      361 gtcgcagcac ttgcaacttg aggtatgacg agtatagcca gttaccgggc tggtctgggt
      421 tattgcatct gcaaaaagca aactactgat ttatttatca gcggtggagc tttgcttttt
      481 ttccgacgtg atcgatttct cctttgagaa attgaggacc tgctattacc tgaaataaag
      541 agatgaataa aatgtcagaa ttaaaaattg ccgttagccg ttcttgcccg gattgttttt
      601 ccactcatcg tgcatgcgtg aatattgacg aaagtaatta tattgacgtt gccgccatta
      661 ttttatcagt caatgatgtt gaacgtggaa aactcgataa aatagacgct actggctatg
      721 gcattcctgt ttttattgca acggaaaatg aagaacgtgt accagcagaa tatctgccac
      781 gtatttctgg tgtcttcgag cattgcgaat cacggaaaga gttttatgga cgtcagttag
      841 aaaccgctgc tagccattat gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg
      901 attatgtcaa tcagggcaat agcgcttttg actgcccggg tcatcaggga ggtgagtttt
      961 tccgtcgtca tccggcaggg aatcagtccg tggagtactt cggagaaacg ctgttccgtt
     1021 ccgacttatg caacgcagac gtagcaatgg gcgatctgct gattcacgaa ggcgcgccat
     1081 gcattgcaca gcaacatgcg gcaaaagtgt ttaatgccga taaaacatac ttcgttctaa
     1141 atggcacttc atcttctaac aaagtggttt taaacgccct gctaacaccg ggtgatctgg
     1201 tgctgtttga tcgcaataac cacaaatcta accaccacgg agcgttgcta caggctggtg
     1261 caacaccggt ttatctggaa acggcacgta acccgtatgg ctttatcggt ggcattgatg
     1321 cgcactgttt tgaagaaagt tacctgcgtg agctgatcgc ggaagtcgca ccgcagcggg
     1381 caaaagaggc tcgtcctttc cgcctcgctg tgattcagtt aggcacctac gacggtacga
     1441 tttataacgc ccgccaggtg gtggataaaa ttggtcatct gtgtgactac attctgtttg
     1501 actccgcatg ggttggctat gagcaattta tcccgatgat ggctgactgt tcgccgctgt
     1561 tgctggatct taatgagaac gatccgggta ttctagttac gcaatccgta cataaacagc
     1621 aggctggttt ttctcaaact tcacaaatcc ataaaaaaga cagccacatc aaagggcaac
     1681 agcgttatgt accgcacaaa cgcatgaaca acgcctttat gatgcacgcc tccaccagcc
     1741 cgttctatcc gctgtttgcc gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg
     1801 gtcgtaatat gtggatggat tgtgtggtaa atggcattaa tgcccgcaaa ctgatcctcg
     1861 ataactgtca gcatattcgt ccgttcgtac ctgaactggt ggatggtaaa ccctggcagt
     1921 cgtatgaaac agcgcaaatt gcggttgatc tgcgcttctt ccagtttgta ccaggggaac
     1981 actggcattc ttttgaaggc tatgcagaga atcaatactt tgtcgatcct tgcaaactgt
     2041 tgctgacaac cccaggtatt gatgcacgta acggcgaata tgaagcgttc ggtgtacccg
     2101 cgacgattct tgctaacttc ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta
     2161 acgccatcct cttcctgctg actccggcag aagatatggc caaacttcag caacttgtgc
     2221 cctgctggta cgcttcgaaa aactgcttga gtccgacgcg ccattagcag aagtgctgcc
     2281 ttccatctac aaacagcatg aagagcgcta cgccggttat accctgcgtc agttgtgcca
     2341 ggaaatgcat gatttgtatg cccgccacaa cgtgaaacaa ctgcaaaaag agatgttccg
     2401 caaagcgcac ttcccacacg tcagcatgaa tccgcaagaa gccaactacg cctatttacg
     2461 cggtgaagtg gagctggttc gtctaccgga tgccgaaggt cgtatcgctg ccgaaggggc
     2521 gcttccttat cctccgggtg tgctgtgtgt tgttccgggt gaaatctggg gtggtgctgt
     2581 tttgcgttac ttcagcgctc tggaagaagg gatcaacctg ctgccaggtt ttgcaccgga
     2641 gctgcagggt gtctatatcg aagaacatga tggtcgtaag caagtttggt gctatgtcat
     2701 caagcctcgt gatgcgcaaa gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa
     2761 tcgaacaaaa tgggcgtcgt tcagttaacc atactgacga tggtcaacat gatgggctcc
     2821 ggtatcatca tgctgccgac aaagcttgcc gaagttggga caatttcaat tatctcctgg
     2881 ctggtgacag ccgtaggctc aatggcaccg gcatgggcat tcgcaaaatg cggtatgttc
     2941 agccgtaaat caggtggtat gggcggttat gccgagtatg ctttcggtaa atccggtaac
     3001 tttattgcga actataccta cggcgtctca ctgctgattg ctaacgtcgc gattgctatt
     3061 tcggcggttg gttacggcac cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt
     3121 cttgcgacca tcggggtgct gtggatttgt accgtggcta actttggtgg tgcgcgcatt
     3181 accgggcaaa tcagtagcat taccgtgtgg gggggcatta ttccggtggt aggcctgtgc
     3241 attatcggct ggttctggtt tagcccgacg ctttacgttg attcctggaa tccgcatcat
     3301 gcaccgttct tcagtgcagt aggttcttcc atcgctatga cgctgtgggc ttttcttggt
     3361 ctggagtctg cgtgtgcgaa tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc
     3421 gcggtactcg gcggtacgtt aggtgcggcg gtgatttata tcgtctccac caacgtgatt
     3481 gcagggattg tgcctaatat ggagctggca aattcaacgg caccatttgg cctggccttc
     3541 gcacagatgt ttacaccaga agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc
     3601 tgcggttcgc tgcttggctg gcagttcacc attgcccagg tgtttaaatc ttcatctgat
     3661 gaaggctact tccctaaaat tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga
     3721 atgttgacca ttgtgattat ccagagcggg ttggcgctga tgacgattag cccgtcgcta
     3781 aacagtcagt tcaacgtgct ggttaacctg gccgtggtga ccaatatcat tccgtatatt
     3841 ctgtcaatgg cggcattagt cattattcag aaggtcgcta atgtgccccc ctcaaaagcg
     3901 aaagttgcaa actttgttgc ttttgttggc gcgatgtata gcttttatgc gctgtactca
     3961 tccggggaag aagccatgct gtacggttcc atcgtgacct tccttggttg gacactgtat
     4021 ggtctggtat caccacgctt tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa
     4081 aagggcgatc ttgcgaccgc tcttttttta ttaaatgtgt ttacgcgttt ttcagaactt
     4141 cgctaacaat ctcaaccgct tctttctcaa tctgcttgcg atgttcttca ccgaggaagc
     4201 tttcgcagta gatcttatat gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt
     4261 tgtcagtcat cactttcaga ccgccaatag aagcaccgtt gcccggagca gcagtcaggc
     4321 gcgcggtgat cgggtcacct gccagggtgc tggcgctcac catttccgga gacagcttag
     4381 acagcgccgc tttttgtgcg gaagtcgcag ctgcctgcaa acggttgtag ctcggcgcac
     4441 caaagcgttt tgccagttcg ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga
     4501 tttccgccgc cagcagacac ataatgatgc cgtctttgtc ggtggaccac ggcgtgccgt
     4561 cgaaacgcag gaaggaagcc cctgcgctct cttcgccgcc aaagccgaag ctgccgtcga
     4621 acagaccatc aacaaaccat ttgaaaccta ccggtacttc taccagctta cggcccaagt
     4681 cattgaccac acggtcgatc atcgcagaag aaaccagcgt tttaccgaca gcaacatctt
     4741 tgccccactg cggacgatgc tggaacaggt agttgatcgc caccgccagg tagtggttcg
     4801 gattcatcaa acctgccgga gtgacgatac cgtgacggtc ataatccggg tcgttagcaa
     4861 acgccagatc gaacttatca cgcagtgcca gcagacccgc catcgcacac tcggaggagc
     4921 agtccatacg gatcgcaccg tctttatcga ggtgcataaa gcggaaggtt tgatcgacct
     4981 gatcgttaac gatggtcagg ttgaggttgt aatactcgcc aatgcgcttc cagtattcga
     5041 taccggaacc gcccaacgga tcaacgccca gcgtcaggcc cgctttctga atcgccgcca
     5101 tatcaacgat atcggtcagc ccttccacga acggctgcac cagatcctgc tctttcacat
     5161 agccggatgc catcgcttcg tcgagggaga tacgcttcac gcctttcagg ccatcggcca
     5221 tcagtgcgtt ggccttgtct tccaccactt tggtgacgtt agtatcagcc gggccaccat
     5281 ttggcggatt gtacttgata ccaccatctt ccggcgggtt atgggacggt gtaatcacga
     5341 taccgtctgc cagcgggcca ccttttttat tgtgaaccag gatggcattg gaaacggcag
     5401 gcgtcggagt gaagccattg ttttcctgca caatgacatc aacgccgttc gctgccagca
     5461 cttccagtac ggaaatgaac gcaggctcgg acagggcgtg agtatcttta cccacatagc
     5521 aagggccagt gatgccgttt ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa
     5581 tgtgcggctc gttaaagctg tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt
     5641 tcaccgcgtg ctccgcattc cctgcttctg gtttcagtac ataatattgc gccgtcagtt
     5701 gggcgacgtt aatcaaatca ctctgttgtg caggttggcc tgcacgattg tggattgcca
     5761 ttgctttgtc ctttgtctgc aacgttttag atagttccgc aaaccttctc aatcaattcc
     5821 gccgggaatt gcatcgactg catgatgtgt tcgatcatgc tgcatttacg gccggtgttg
     5881 gtgttggtga tcacccaata cggcgtgcct ggcacatgtt tcggcttggt ctgattacca
     5941 tttttcagca gcgtttgttc atctgccgca aagtacacgc gtgtacgacc gtgcaacgat
     6001 tccgttgctt cggcaaacgc ctgggcgtca agagaatata gtgtagacaa cagcagcata
     6061 aagcgattga ccgctcgctt ttgctctgcg tattcgtccg aaagcagaag ttcacgcatt
     6121 gcgcgaacct tgtctttaat cgttttgacc ggcttcgctt cgacgatagc aggtgacgca
     6181 acgcgaacct ctttcgtcac cggagcagca ggctgtgatg cggcggaaaa tttcaacata
     6241 cgccgtaaaa tgtcggatgc gctctcgccg atatgcttag tgtggctggc aatatagctg
     6301 tagagttcat catcaacttc aatcgttttc atcttaatcc agtgcagtgt cttagctgaa
     6361 tacaagtcgc caagagtata tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg
     6421 cagaaaaact cggaataaac cttatttgtt gcagccctgt ggcacaatgg tcaacataat
     6481 accctaaccc gacaacgcga aaaaaagaac tttgccatga aattaaatat ccgcgcgcaa
     6541 actgcacaaa accagcacaa taattctccc atcgttcttg tccatggtct gtttggtagc
     6601 ctcgacaacc ttggcgtact ggctcgcgat ctggtaaacg atcacaatat catccaggtt
     6661 gatatgcgta accacggtct ttcaccgaga gagccggtaa tgaattaccc ggcgatggcg
     6721 caggatcttg ttgataccct ggatgcactg cagatcgaca aagcaacatt tatcggtcac
     6781 tccatgggcg gtaaagcggt aatggcactt actgcactgg cccccgatcg catcgataaa
     6841 ctggtggcga tcgatatcgc accggtcgac tatcacgtac gccgtcatga tgagattttc
     6901 gcagctatca acgcggtcag tgaatcggac gcacaaactc gccagcaagc agcagcaata
     6961 atgcgccagc atcttaatga agaaggggtg attcagtttc tgttgaaatc ttttgttgac
     7021 ggggagtggc gctttaacgt gccggtattg tgggatcagt atccgcatat tgtgggttgg
     7081 gagaaaatcc cggcatggga tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat
     7141 gttagcgagc agtaccgtga tgatttactg gcacaatttc cacaggcacg ggcgcatgtg
     7201 attgcaggcg cgggtcactg ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc
     7261 cgtcgctatc tcaatgatta acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg
     7321 cgcgtgggcg ttggcgtgac tcatcgcgct gatgtattat ggcgcgctat ccatccgggc
     7381 agaggcctgg ctgattgttt cccccgaagt caccaagatc atggccaaag aacaaacgga
     7441 ccgtacgaca ttagatctgt tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc
     7501 gctttcgcgc gatgaacagc tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt
     7561 acgtggcctt aagcgtgtcg aactgaagct gaacgcggaa gctgtcgagg cgctgaacga
     7621 gctggcggaa tcgcgtaata tgagccgtag cgaactgatc gaagagatgt taatgcagca
     7681 actggcggcg ctgcgtagtc agggcatcgt ttaaatttcc actttcatgt agcacagtgt
     7741 gcagtcctgc tcgtttgctg attccgcaag actgcctgtt ctgctatgat tgcctttatc
     7801 cgtgggcaat tttccacccc catttcaata agtttcaaga ggttatttca ctcatggcta
     7861 tcactggcat ctttttcggc agcgacaccg gtaataccga aaatatcgca aaaatgattc
     7921 aaaaacagct tggtaaagac gttgccgatg tccatgacat tgcaaaaagc agcaaagaag
     7981 atctggaagc ttacgacatt ctgctgctgg gcatcccaac ctggtattac ggcgaagcgc
     8041 agtgtgactg ggatgacttc ttcccgactc tcgaagagat tgatttcaat ggtaaactgg
     8101 ttgcgctgtt tggttgtggt gaccaggaag attacgccga atatttctgc gacgcattgg
     8161 gcaccatccg cgacatcatt gaaccgcgcg gtgcaaccat cgttggtcac tggccaactg
     8221 cgggctatca tttcgaagca tcaaaaggtc tggcagatga cgaccacttt gtcggtctgg
     8281 ctatcgacga agaccgtcag ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac
     8341 agatttctga agagttgcat ctcgacgaaa ttcttaatgc ctgatgtgat gcggcgtaga
     8401 ctcatgtcta cgccgtatta atagataatg ccaatcaaaa taattgctac aaatttgtaa
     8461 cttttgctgt tgtacctata caatgtccca gtgttcaagt ggccttgccg ttgtaaatgt
     8521 aagctgtgcc acgtttttat taacaatatt tgccagggac ttgtggtttt catttaggcg
     8581 tggcaattct ataatgatac gcattatctc aagagcaaat tctgtcactt cttctaatga
     8641 agtgaaccgc ttagtaacag gacagattcc gcatgactga taacaatacc gccctaaaga
     8701 aagctggcct gaaagtaacg cttcctcgtt taaaaatcct ggaagttctt caggagccgg
     8761 acaaccatca cgtcagtgcg gaagatttat acaaacgtct gatcgatatg ggtgaagaaa
     8821 ttggtctggc tacggtatat cgcgtactga accagtttga cgacgctggt atcgtcaccc
     8881 gccacaattt tgaaggcggt aaatccgtat ttgaactgac acagcaacat caccacgatc
     8941 acctgatttg cctcgactgc ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc
     9001 gtcagcgtga aattgccgca aaacatggca ttcgcctgac taaccacagt ctctatcttt
     9061 acggtcactg tgccgaaggc gattgccgcg aagatgagca cgcgcacgaa ggcaaataag
     9121 ccagcctgaa cgagaaaagc caacctgcgg gttggctttt ttatacaagg gaaataagaa
     9181 aagcgagagt tacagctctc acttatttgt tattactgcg tatttcctgc cagaccttat
     9241 cacaatcggc tttcaccgcc tgatcatttc cggtttgcgt tgcacgcaga cactgctgat
     9301 aatccagtac gcgtacactt tcctggtcgg caaactgctg atgttgtttc tctttcttca
     9361 gcacgtttaa cacgctctgg caggcttcaa ttttttctgg cgaaccttgc gcggtgttga
     9421 tacaggcgct gtatgcctct ttcagacggc tgtcttcctg tggtgcggtt gattgcgcac
     9481 aagctaccag ccccgatgcc atcatggcga tgagaatcag ttttttcatt gaatgcggca
     9541 ggcacaaaac ctgccgcgtc gggcatcaga agatggtgaa tggtgcgatt accataaatt
     9601 tcacgtcacg ctcatcctgg aagatgttac cgtaaccgcc gccccagctt gggatgtcgg
     9661 agtggttgtc gtattcggtg aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct
     9721 gaatggtgta gaccgcatcc aggctgtagg cagactcttc aatagtccgg tttttgtcgt
     9781 agtacgcatc cggattgctc tgccaggtcg caggtttagc atcccatgcg taaacgtagg
     9841 aagcgccgat ggcgaagcct ggaagattcc agtttttcag gtcatacatc gcactgaaga
     9901 agaccgcttt ttcgccgtta gcgttgaagt cggaacggtt atcccaccag atatccaggc
     9961 ggccgttgga ggaagcgtag gttggggtca tacgttgcag gaagtatccc tgctgactgt
    10021 cagccttaat ccaggtgcct tcgaggcgca aatccactac gtcagccgcc cggtaaccaa
    10081 aggtcaacgc ttgcagccag gcggtgccgt cataaagatc gttgacgctg cgatcgtcaa
    10141 ctttatcgcg ggtaccatag aactggtagc tggtggttaa cgggctaccg gcgatatcaa
    10201 atttgtagct ggctttggca aaatattgat cgatataccc ttccgcctga ccaaatgccg
    10261 cttccagtac gaagttattt ttgaagtcgt atttcgcacc gatggagtgc agataatcaa
    10321 ctttggtggt tttatcgttc tgataaaact catccatttc cagatgccac ggcgctttgt
    10381 attcgttggt ccacatgtag gagaaactca aggcaccagc atcgccgtaa tcaaaattcg
    10441 ccccggcttc cgccccctga taagtacccg gcataaagct ccagtgcggc gctaacagcg
    10501 tttgaccggt tggctgaatg taacctgccc tcgcccaaac cggaccgtat ttaaatttgg
    10561 ccgcagcttt atacaggctt ataccgcttt tgtcgccgga ccagtcttcg tcataggctt
    10621 tattactttt tgaaaacgcg atttcgttcg ggtgggagct gtcgccgttt tccgccattt
    10681 caatcgccgt aaacgcagca atatcaaggc cgaacatatc agcagcataa ccggactgaa
    10741 aatcgaggtt ggcattccag gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg
    10801 taacatcttt acggtcgcgt tcacgctgcc agtaatagat accgccggtt aaggttgaat
    10861 cgtcgatgaa tccttcggcg cgagcctccg gcattgccat aaagcccgac attgctgtaa
    10921 caccggcaat agccagcgcc agcgtactac gtttgccact aaacgtacgc atgggttaat
    10981 cctctttgac gtataaattg ctgcaccaaa ggtgaacagc gaaaaaaaat aaaaattaaa
    11041 aaagtag
//
LOCUS       DAZXUK010000125        11046 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZXUK010000125 DAZXUK010000000
VERSION     DAZXUK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11046
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.125"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            162..812
                     /gene="csgD"
                     /locus_tag="ACH3L8_002529"
     CDS             162..812
                     /gene="csgD"
                     /locus_tag="ACH3L8_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFT4085599.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            817..1206
                     /gene="csgE"
                     /locus_tag="ACH3L8_002530"
     CDS             817..1206
                     /gene="csgE"
                     /locus_tag="ACH3L8_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFT4085600.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1231..1647
                     /gene="csgF"
                     /locus_tag="ACH3L8_002531"
     CDS             1231..1647
                     /gene="csgF"
                     /locus_tag="ACH3L8_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFT4085601.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1674..2449
                     /locus_tag="ACH3L8_002532"
                     /pseudo
     CDS             1674..2449
                     /locus_tag="ACH3L8_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
     gene            complement(2513..3004)
                     /locus_tag="ACH3L8_002533"
     CDS             complement(2513..3004)
                     /locus_tag="ACH3L8_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFT4085602.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3106..3660)
                     /gene="ycdY"
                     /locus_tag="ACH3L8_002534"
     CDS             complement(3106..3660)
                     /gene="ycdY"
                     /locus_tag="ACH3L8_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFT4085603.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3684..4421)
                     /gene="ycdX"
                     /locus_tag="ACH3L8_002535"
     CDS             complement(3684..4421)
                     /gene="ycdX"
                     /locus_tag="ACH3L8_002535"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFT4085604.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4476..5414)
                     /gene="ghrA"
                     /locus_tag="ACH3L8_002536"
                     /pseudo
     CDS             complement(4476..5414)
                     /gene="ghrA"
                     /locus_tag="ACH3L8_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            5649..5736
                     /locus_tag="ACH3L8_002537"
     tRNA            5649..5736
                     /locus_tag="ACH3L8_002537"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5683..5685,aa:Ser,seq:gga)
     gene            complement(5886..6167)
                     /locus_tag="ACH3L8_002538"
                     /pseudo
     CDS             complement(5886..6167)
                     /locus_tag="ACH3L8_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(6449..7453)
                     /locus_tag="ACH3L8_002539"
     CDS             complement(6449..7453)
                     /locus_tag="ACH3L8_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HFT4085605.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(7525..8085)
                     /locus_tag="ACH3L8_002540"
                     /pseudo
     CDS             complement(7525..8085)
                     /locus_tag="ACH3L8_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(8170..8367)
                     /locus_tag="ACH3L8_002541"
     CDS             complement(8170..8367)
                     /locus_tag="ACH3L8_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFT4085606.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(8379..8870)
                     /locus_tag="ACH3L8_002542"
     CDS             complement(8379..8870)
                     /locus_tag="ACH3L8_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFT4085607.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(8867..9241)
                     /locus_tag="ACH3L8_002543"
     CDS             complement(8867..9241)
                     /locus_tag="ACH3L8_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFT4085608.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(9288..9662)
                     /locus_tag="ACH3L8_002544"
     CDS             complement(9288..9662)
                     /locus_tag="ACH3L8_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFT4085609.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(9825..10046)
                     /locus_tag="ACH3L8_002545"
     CDS             complement(9825..10046)
                     /locus_tag="ACH3L8_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFT4085610.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(10109..10585)
                     /locus_tag="ACH3L8_002546"
     CDS             complement(10109..10585)
                     /locus_tag="ACH3L8_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFT4085611.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(10601..>11046)
                     /locus_tag="ACH3L8_002547"
     CDS             complement(10601..>11046)
                     /locus_tag="ACH3L8_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFT4085612.1"
                     /translation="SACAPETGLQQLVATPVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT         2825 a         2722 c         2692 g         2807 t
ORIGIN      
        1 ggctagattg aaatcagatg taatccatta gttttatatt ttacccattt agggctgatt
       61 tattactaca cacagcagtg caacatctgt cagtacttct ggtgcttcta ttttagaggc
      121 agctgtcagg tgtgcgatca ataaaaaagt ggggtttcat catgtttaat gaagtccata
      181 gtattcatgg tcatacatta ttgttgatca ctaaaccttc tttgcaggcg acagctctct
      241 tgcagcacct taaacaatcg ctagcaatta caggaaaatt acataatatt caacgttctc
      301 tggacgatat ctcttcaggc tctattattc ttctggatat gatggaagcg gataaaaagc
      361 ttatccatta ttggcaggat actttgagca ggaaaaacaa caatatcaaa atattgttgc
      421 taaatacgcc tgaagattac ccgtaccgcg acattgaaaa ctggcctcat atcaacggcg
      481 ttttttatgc catggaggat caagaacgtg ttgtcaatgg gttgcaaggc gtcctgcgtg
      541 gcgaatgcta ctttacgcaa aagcttgcca gttacctgat tacacattct ggtaactatc
      601 gttataacag cacggaatca gccctcctta ctcatcggga aaaagagatc ctgaataagc
      661 tgcgtatcgg cgcgtctaat aacgagatcg ctcgttcgtt gttcatcagc gaaaatacgg
      721 ttaaaacgca tctttataat cttttcaaga agatagccgt aaaaaaccgg acacaagcgg
      781 tttcctgggc aaacgataac ctcaggcgat aaagccatga aacgttattt acgctggatt
      841 gtggcggcag aatttctgtt cgccgcaggg aatcttcacg ccgttgaggt agaagtcccg
      901 ggattgttaa ctgaccatac tgtttcatct attggccatg atttttaccg agcctttagt
      961 gataaatggg aaagtgacta tacgggtaac ttaacgatta atgaaaggcc cagtgcacga
     1021 tggggaagct ggatcacaat aacggtcaat caggatgtta ttttccagac ttttttattc
     1081 ccgttgaaaa gagactccga gaaaactgtc gtctttgcac tgattcaaac tgaagaagca
     1141 ctaaatcgtc gccagataaa tcaggcgtta ttaagtacgg acgatttggc gcatgatgaa
     1201 ttctaaataa aaaattgttc ggaggctgca atgcgtgtca aacatgcagt agttctactc
     1261 atgcttattt cgccattaag ttgggctgga accatgactt tccagttccg taatccaaac
     1321 tttggtggta acccaaataa tggcgctttt ttattaaata gcgctcaggc ccaaaactct
     1381 tataaagatc cgagctataa cgatgacttt ggtattgaaa caccctcagc gttagataac
     1441 tttactcagg ccatccagtc acaaatttta ggtgggctac tgtcgaatat taataccggt
     1501 aaaccgggcc gcatggtgac caacgattat attgttgata ttgctaaccg cgatggtcaa
     1561 ttgcagttga acgtgacaga tcgtaaaacc ggacaaacct cgaccatcca ggtttcgggt
     1621 ttacaaaata actcaaccga tttttaagcc ccagcttcat aaggaaaata atcatgcagc
     1681 gcttatttct tttggttgcc gtcatgttac tgagcggatg cttaaccgcc ccgcctaaag
     1741 aagccgccag accgacatta atgcctcgtg ctcagagcta caaagatttg acccatctgc
     1801 cagcgccgac gggtaagatc tttgtttcgg tatacaacat tcaggacgaa accgggcaat
     1861 ttaaacccta cccggcaagt aacttctcca ctgctgttcc gcaaagcgcc agggcttaca
     1921 aaacctgctt aacgagcgca aggttattcg tgcggcacaa gaaaacggca cggttgccat
     1981 taataaccga atcccgctgc aatctttaac ggcggcaaat attatggttg aaggttcgat
     2041 tatcggttat gaaagcaacg ttaaatcggg cggtgttggg gcaagatatt ttgggatcgg
     2101 tgcagacacg caataccagc tcgatcagat tgccgtgaac ctgcgtgtcg tcaatgtgag
     2161 taccggtgag atcctctcct cggtgaacac cagtaagacg atactttcct atgaagttca
     2221 ggccggggtt ttccgcttta ttgactacca gcgcttgctg gaaggggaag tgggttacac
     2281 ctcaaacgaa cccgttatgc tgtgtctgat gtcggctatc gaaacagggg tcattttcct
     2341 gattaatgat ggtatcgacc gtggtctgtg ggatttgtaa aataaagcag aacggcagaa
     2401 tgacattctg gtgaaatacc gccatatgtc ggttccaccg gaatcctgac agataaaaat
     2461 gccggaatta ataatccggc atttactgtt taagcgaaag cgtgagtgcc cctcacgctt
     2521 tatttttgat ttcctgctca cggtgagccg tctttgcact acgcgccgcc agccacaggc
     2581 cgctgttttt cattgcgtaa ccaaatacca gcccaagcgc cagcgaaggt aacaccagtt
     2641 tccagtcacc ctgcccggca aacgtcgcgc atgcgcctat aaacgtcccc ggaacaaatg
     2701 aaagcagcag ctgtttggcc tgaatacaca tcagaaacgc gacaataccg gttatgacat
     2761 aaccgagaat ttccagatgt ggtgccagtg cactaccgta aataatgacc atcgcccaca
     2821 ccacgccact aagcagggtt gcagcggaga tcgccagccc tttcagccca ccttgcgggc
     2881 aagcaaagta ggccgtacag cccaggaagc ccgcccagct tagtaagccc agggaaacag
     2941 ccacccatcc ccagataccg gagagaatgc ctgttgtgat tgcaatagag agaagtatgt
     3001 tcatgccgcg catgatagca gaaaacgcgg ccatgaatga gatcgagcac acattttatg
     3061 caacaacaaa ttatctgttg cagttatgag tgacgatgat cacatttact cttcagaatc
     3121 ttcttccagc tcgtcccaca ttgcactaat ggcatcacgg gttagcggtg ccatggtgcg
     3181 ccagaaaggc gtggttgcat gggcctccac tttgccgagg aacgcaccac accagggtaa
     3241 cagatactca ctgaacagtg tttccagtgc ttcgctctca tcttccgttg actgatcttc
     3301 cagccaggaa gccgcaagca gcaatgtgcc gatgtgatcg gctggcgtat ccgccagtag
     3361 catccctcgt tcggaaagaa aagcgcgcac ttccgcttcc gttgcgtcct caacccatgc
     3421 gctacgatat ggcggcacgg cacattcatc gccgataaac aacgcattgt aatcggcaga
     3481 gacttgcgtc atatcacagc ttttctgtaa acgcgtcagt aactcatcct gctccagtgg
     3541 ccagttcgca gccagtttcc cctcacgaat cagagtaaac agcggcacca gtaaaggatc
     3601 ttgcggttgg cggtaataga gcgaacccag cacacgacag aggatagaaa actcgttcat
     3661 taatatattt ccattactag taattaaagg tctgcaaatt ccgcaatcgg tgccataccg
     3721 cgagattcaa ggaagttcag taagcggcgc ggagaaacat tcaaaatgcg ctctagcgga
     3781 aaatctaccg cgtcgaggat tttaagacac tcttcaaatt cccccatggt aaacgcggtg
     3841 tgagaatccg agcctaatgc cacccaacct ccagcatcac gtaccgctgc agctaccgca
     3901 cgacagttgt cttcactgcc cttacgtgag tgtaaaaatg aggaattatt gatttccagc
     3961 gccacctgat gtttcgcggc tgcttcagca acggctttaa catctatttc atatttcgga
     4021 tttccgggat ggcttattat atgcacattg ccgctggcga tagtggagat catcgcttgt
     4081 gtattggtcg ctttgtcatg tggcgcaaaa accggctcat gaaaaccggc aataatcaga
     4141 tccagcgagt caaacatttt accgctgcag tcaatttcac catcaacatt tttaatgtta
     4201 gcttcaatgc cgcgcaggat ccctacccca tcaaccactc gcggccagat acgcatgtta
     4261 atgaagtgcc agtgatgcgg cgcatcttcc atatccgggc catgatcggt gatcgcaaaa
     4321 agtttaatgc ccttttgttt ggcctgggca atgtaatcac tcaatgtgct atatgcatgt
     4381 gtgctggcaa cggtatgcat atgaaggtcg acgggataca taactctctc ctgtattcaa
     4441 ttcccgccaa ggatagcagg aatcctgatg ctttattagt agccacgtgc gcggtcgact
     4501 tgcccgcaga ccctctcccc tttttcgagc tgggcaatag tgcgagaaat gtactccaca
     4561 gcttcagcgg gacgggtaat cgcggcgaca tgtggtgtta tcgtcacgcg tggatgttgc
     4621 cagagcggac tttcaggcgg taagggttca cgattaaaaa catccagcat tgcgccttta
     4681 actttcccgc tatccagcgc cgcgagcagg tcatcttcca caacatgaac accacgcgcc
     4741 aggttgagga gatacgcgcc atcaggtaat ttttcgagta attgttgatt aataatgccg
     4801 acggtttcag gggtattcgg taacaaatta atcaataccc gacattggct cagaaatgca
     4861 gacagttctt cccgtccggc aaagctttgc acgccaggcc acgatttacg ggttcgactc
     4921 cagcaacgca gcggaaagcg ctaggtttgc agactctgag caactttaca gcccagtacg
     4981 cctgcgccca aaatgccgat ggtaaaatct tcccgatgat attcaggcag cggttgccaa
     5041 tgcgaactat tttgctggat gcgataatcg tcaaaacgtc gaaaccaatg cagcacctga
     5101 ctgacagcat attcctgcat ttgctcgccc ataccggtat cttccaggcg aaaaagtgga
     5161 acagaagggt tcagcatttc agggtgtgcc tgtagcttgc tcaaaataga atcaacaccg
     5221 gccccgagtg cgaacaccgc tttaagatcg cgccctgcca gcatttcaac aggaggatgc
     5281 cagactaaaa cataatcagc agagtcatta tctccgcttt tccatgccct gactcttgcc
     5341 tgaggaatag ctttgcgcag tgcctcaatc caccattggg tatcgaacgt tgggtgataa
     5401 aagatgatat ccatactgac tcccgaaaag cgttgtgcga aatttattcg cacttatcgt
     5461 tatgatctac aaaggccacc agcataacaa atccgtggtc ggtggcaaaa aaagcagatt
     5521 tcgcttatta aaaccacaca ttgattgaat gtttgaataa acgcgcgatt ttttcaaaaa
     5581 gtttgttgac ctcaggtcat gatttcccta aattagcgcc cgttccagca agacaggaac
     5641 gacaatttgg tgaggtgtcc gagtggctga aggagcacgc ctggaaagtg tgtatacggc
     5701 aacgtatcgg gggttcgaat ccccccctca ccgccagatt ataagtgcat gttattgttt
     5761 aacatgcgct tttttatttt tccttccaga cagtatgcca tcgggtatgc catctgaaac
     5821 cggtttatat gccccgcgat catatcttat cggacaattt ccgatctttg agcatactgg
     5881 caaagctata ctgccagcat atcaggatag tgttttgcga taatatcatt cagtctgtca
     5941 accagttctg gttttctgaa cgtgatatgc gcggagccct tcttaaagta tctgatgctg
     6001 aacatctcat cttcatagct ctctttaccc tgaacagact gaatgtgatc atccagacga
     6061 agggtgatgt ttccccggtt gtcggggatt ggtttaccgc tgaacaggtg caacattctt
     6121 tccagatcgg cgagtcggtc agcctgttgt ccggtgataa gatgaaatct atggtcaccc
     6181 ccgtttttgc aacactgatt tttgattaat ggttggcttg cttaaatcta tccggcgtct
     6241 ctgtgggcaa ggatgcccgc gccccgatga gttccgcacc tgataatcct aaaaatcccc
     6301 tcggcttcag tgagccattt catctgttag gtttttaatc aggtcgattt gccgttcatg
     6361 tcatcaataa tcagtcttcg caaaaccaga aaggtaactt gtatcttatt gtttaaaata
     6421 aactaattga gttatatcag catctatatc aggcctgata ttcttttttc tgagtagtga
     6481 ttgcccaggc aattcgagcc agtttatttg ccagagcgca ggtaaccaca ttcgaatgct
     6541 tcttgttgag ttgctcccga acccactcag caagttttcc atgcttgcgt tcaagtcgca
     6601 ttatgaatga gcgggcacac tgaaccagta accggcgtaa atttttatca cctcgcttac
     6661 tgatacccaa cagagtcgat tttcctccag tgctatactg ccggggaaca agtcctgtgg
     6721 aagctgcaaa atccctgctg catgagaact gtttcccgtc accaagttgc gacgatagta
     6781 agctggcagt aatcggacca acaccaggaa ttgtcataat gcgctgagcg gtatcatcag
     6841 cgttgattga ctgacttaat tctgattcca gctctgctat ttgctcaaca aggtaaaagt
     6901 agtgggtatg caacctcacc aaaaggcggc tcagatattc ggggatttcg tgttcagcca
     6961 acacgagaga caaccgtttt attacagcat cacctatcgg caggctgatt ccaaattcca
     7021 gcaagaaacc gtggatctgg ttggttgttt tcaccttatc tctgatgagc gattctctga
     7081 cccggtgtaa cgccctcatg gcctgctggg tctcagttct tggctggaca aaacgcattg
     7141 atggtcgtga agccgcttcg catatggctt ctgcatcaac aaaatcattc ttattacttt
     7201 tgacaaacgg gcgaacgaac tgtggagaaa tcagcttagt ttcatgccca aaatcagaga
     7261 tacagcgagc cataaagtgt gcacccgcac aggcttccat tacaacaact gagggcggac
     7321 atgtcgccag aaactgcatc agtttagtac gggtaaattt tttacgcaga agagccttac
     7381 cagacttgtc ttgacaatga atgtggaagg agtgctttcc gagatcgata ccaataagtg
     7441 tgatgttctg catgatggtc tctccgttaa agggatttac cttaacagcg tactgctgtt
     7501 aaggtggggg tgaccatatc attatcccca ccggtcccat ctcaccagat tgttgacgat
     7561 aattttactg ccaaatttac aggggcaatt ggttttgtaa ttccagttca gccctctgaa
     7621 gacgttgatc acccctcgct caaacacctc atccttattc tggtgcagtt gttcaaatgt
     7681 gctcagaatg ttcgcttcgc tgatttccgg aaaattatcg tactccagtg accggtacca
     7741 tgtatcacgg gcctgcgtat ccattaatga cagcatcccg gaacgttgca tcaggtcacg
     7801 ccagatttcg cgatcaagat tccgggtaat ggctttcatt gctgtttcgg gtttctccat
     7861 cagccagcag ccgcagcgaa aatcctgttt catggcccag tccggggcag ttttacctcc
     7921 gatgctttcc gtcagcaccg agatttcagc cagttgtcgg ataagtgtgt cgatttgcaa
     7981 cagtgtactc tttcgtacag ccagaatgtg gcgaatgctg gttgagcaga taacgtccgt
     8041 atgcccgttg accacttccg gacgggagat tgtgatatct gacataatcg atattccttt
     8101 ttgcagataa atgaaaacgc cagccctgtg caggctggcg tcagaggaat gaagtctggt
     8161 gagtgagctt caccggttct ggtgcagaag ctgcagatga cgcagggtac ggacatcctc
     8221 ccgggcctgt tcgatacagg gcaacagtgc tgctgaatcc gttttatcct catcgttgtc
     8281 aaagataatt cccgatccgc agtcgatatt gtcctgcagc cacgcaatca gaatatccag
     8341 cgctgtttcc gtggttaatg atttcatgtt gttcctggtt accagtcgaa agtgggtaaa
     8401 cccggcaggc attcagcgct ggcatccaga tgaatgagac tgacgccata acgccggatg
     8461 agtgtgacga ccagacgacg gaacgttttt gacagcccca ggcgtttaag ccgcagaacc
     8521 gggtatgacc acgcatcggt acgtaacaga tatccgctac ctgtaaaatg aatccattct
     8581 gattcaccga agtcactggt ctggtgtgac agcgagtaca accaggcgtt gtccgtttcc
     8641 gtgatatgtg cggtactgca gcgtatgccg gtaagggtag caaatggtgg cgtgcattct
     8701 gctgaggtct ggtcagattc atcaacaaca cgaagggagt agccgttatc gcagaccatg
     8761 ttaatcagtt cagcgagctc aataccatcg acgtcaacga caatgcgccc tatgttcagt
     8821 gcctgtacgt taacgctgtc ggcttccagt gtcagggcca gtttcatcat ttcgcctccg
     8881 gatacttacc cagggtaatg ttatttaccg ttctgtaatt gtcgcgggtc atcaggccgg
     8941 tcgcccggcg agcccggagg atatcaatac tgtttattaa ctgagagcgg ggacaggtgc
     9001 tgaatcccgg ctggtcggta cgcaccagcg cgtatttttc cacgagaaaa ttaactgcat
     9061 cacacagtga aatgcctgcc tcaatatgct gctcaatcac acgttcatcg acaaacggtg
     9121 tgtcattcag tgtgaggcca taatgctggt ccagcagtcg gatgagcagt gtctgccaga
     9181 tggtgacggg agacgggcag cgagatacct cccgtacatg tgtgtcggat aatgttttca
     9241 tcttaagttc tctgtaactg gtagtgctaa aggtgagagc ttcggggtta tgaggtggtt
     9301 gcgggtgctg gtgtcggata aacagccaga taaacgtaac cacaggagcc gagggtgtcg
     9361 gcttcgcagg tcagcccttt tgcatacagc gtgacggtat gctgatatcg gggattcagt
     9421 tcaccgctgg tgagcatgag ttccagttgt ttcatcagca gcggaaaggc ctggtccaga
     9481 tggtatgcat ctgcgtcgct gaagcggcct ctgataccgg cgcggtcggc aaggtaatac
     9541 aggcggttac cctcttgcac cagacgagcg ccaaaacagg gcctcactgt gcagggaagc
     9601 ccccaccagg ggcggtcgtg attgtcatcg ggatgagtta tcccggagag tttgtctgac
     9661 acgataaaat cctctctgaa aagtggttaa gaatggttta acgatgacag gtcagccggc
     9721 gcgctgaccg gtttcggtga agatgtatgc attgatgaga atggcctcat ccacagcggc
     9781 gtctgaggtc atctggtcgt actcgtttcc cgggtgacgg aacatcagtt cagggtgaca
     9841 ctcatcagcc ggacatacgg gccaaaactg tccttacggc gttctgcaaa cacggcaaga
     9901 acgccgggaa tatcctgcac ttcacgacca gtatacgctt cagcactgcc gtgccagcga
     9961 tacttaccgg tacagaacgg aaaaagacgg gatgcaggat gctgttggtg aatacgcatg
    10021 gcttcaccac gggtgatgat tctcatgatg tgatacctct gaagacagaa gataaaagtg
    10081 aaaacaggtg tgatgtggtt gtgacgggtt aaagcagacc atgttcggca aaggagaaaa
    10141 cctggttgcc accgactatc agatggtccg gcacccggat atccaccagg cccagtgcct
    10201 gtaccagacg ttcggtgata agccggtctg ccttactggg tgtgacttca ccggacgggt
    10261 gattgtgcgc cagcaccacg gcagcggcat tgtggtacag ggcgcgttta atcacttccc
    10321 ggggatgaac ttccgtacgg ttgatggtgc cggtgaagag ggtttcaccg gcaatcagct
    10381 gattctggtt gttcagatac agcacccgga actcttcccg ctccagtccc gccatgttca
    10441 gaatcagcca ttcccgtgcc gcacgggtgg aggtgaaggc cacgccgggt tcatgaagat
    10501 ggcggtccag ggtttgcagg gcccgcagaa tgagactgcg ctcgccgggc gtcatgtttc
    10561 cgggcagaaa ggaaagctgc tgcattgtcc ctgcctccat tcagtcgatg atgcgcataa
    10621 tggcgctgca ttccggatgc tgcagggcgt aatcccgcag ccggtaataa tggaccgtca
    10681 tggcataaca ctccgtacga caggcatgat gactgtacgt catcagacag gcagcaatgc
    10741 cggcggcttc cgggctcatt tcagcgcggt taccgttcat ggcattgaac agtacccatg
    10801 tttcgtcatc atcgtcatcc ggttcgggtg ccataaatgc cccgccgttg ttcagggtgt
    10861 acagattcca gataccaccg cagtagtctt cacacagacg gtccatccag ccgaagacac
    10921 ggggttccag ggtgacccac tgtggaatga ggccaaaatg ctgcggccag aagctgatgc
    10981 gctgttcatc agggacggga gtggcaacca gttgttgcag gccagtctcc ggtgcacagg
    11041 ccgaag
//
LOCUS       DAZXUK010000126        11025 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZXUK010000126 DAZXUK010000000
VERSION     DAZXUK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11025
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.126"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..110)
                     /locus_tag="ACH3L8_002548"
     CDS             complement(<1..110)
                     /locus_tag="ACH3L8_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFT4085613.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASG"
     gene            complement(236..586)
                     /locus_tag="ACH3L8_002549"
                     /pseudo
     CDS             complement(236..586)
                     /locus_tag="ACH3L8_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(789..1802)
                     /gene="intI1"
                     /locus_tag="ACH3L8_002550"
     CDS             complement(789..1802)
                     /gene="intI1"
                     /locus_tag="ACH3L8_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFT4085614.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1963..2436
                     /gene="dfrA17"
                     /locus_tag="ACH3L8_002551"
     CDS             1963..2436
                     /gene="dfrA17"
                     /locus_tag="ACH3L8_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFT4085615.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            2567..3355
                     /locus_tag="ACH3L8_002552"
     CDS             2567..3355
                     /locus_tag="ACH3L8_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFT4085616.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            3561..3908
                     /locus_tag="ACH3L8_002553"
     CDS             3561..3908
                     /locus_tag="ACH3L8_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFT4085617.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            3902..4741
                     /gene="sul1"
                     /locus_tag="ACH3L8_002554"
     CDS             3902..4741
                     /gene="sul1"
                     /locus_tag="ACH3L8_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFT4085618.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            4869..5072
                     /locus_tag="ACH3L8_002555"
     CDS             4869..5072
                     /locus_tag="ACH3L8_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085619.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            5228..6433
                     /gene="chrA"
                     /locus_tag="ACH3L8_002556"
     CDS             5228..6433
                     /gene="chrA"
                     /locus_tag="ACH3L8_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFT4085620.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            6444..6749
                     /locus_tag="ACH3L8_002557"
     CDS             6444..6749
                     /locus_tag="ACH3L8_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFT4085621.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            6976..7740
                     /locus_tag="ACH3L8_002558"
     CDS             6976..7740
                     /locus_tag="ACH3L8_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFT4085622.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(8233..8817)
                     /gene="mphR(A)"
                     /locus_tag="ACH3L8_002559"
     CDS             complement(8233..8817)
                     /gene="mphR(A)"
                     /locus_tag="ACH3L8_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFT4085623.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(8817..10055)
                     /gene="mrx(A)"
                     /locus_tag="ACH3L8_002560"
                     /gene_synonym="mrx"
     CDS             complement(8817..10055)
                     /gene="mrx(A)"
                     /locus_tag="ACH3L8_002560"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFT4085624.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(10052..10957)
                     /locus_tag="ACH3L8_002561"
     CDS             complement(10052..10957)
                     /locus_tag="ACH3L8_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFT4085625.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT         2286 a         3191 c         3256 g         2292 t
ORIGIN      
        1 ccggatgcct tgtcgctaaa agcgcgatca accttgacgc cttccagttg ccgttccggg
       61 ttctggtcga aggtgctgac cctgatatac ccaatgcgct gtccagtcat ggaattccct
      121 gcaaaatgtc agggaagact ctatgacctt caacgagata tgtcaataaa ttcaaaattc
      181 aatcctatcc tgacgcaatt tacacatggc atctgacatc aggttagggt atgcctcaac
      241 ctgacggcgg cgaatcacaa gcgtccggtt tgacgctggt ttcggtcgca gtgctggccc
      301 gcgcctggaa gcgctgatag cactccagcc cgcagaaatg ctccacgtac tcggcccctt
      361 ccggcgtgaa ggcggcatcg agcgggattt ccttgcagca cacgcagcaa ctggtgctcg
      421 gttcattggc gttcatggtg gtgttcctcc attggttgac gaagccgacg aaggccgccg
      481 ccggcatcgg cctggcgaac aggaaaccct gcaccgtgtc gcaacccgcc tgccgcaacc
      541 acgcaaggca gtcgggtgtt tccacaccct cggctaccac ctccattgtc gttttcagaa
      601 gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatccat
      661 caggcaacga cgggctgctg ccggccatca gcggacgcag ggaggacttt ccgcaaccgg
      721 ccgttcgatg cggcaccgat ggccttcgcg caggggtagt gaatccgcca ggattgactt
      781 gcgctgccct acctctcact agtgaggggc ggcagcgcat caagcggtga gcgcactccg
      841 gcaccgccaa ctttcagcac atgcgtgtaa atcatcgtcg tagagacgtc ggaatggccg
      901 agcagatcct gcacggttcg aatgtcgtaa ccgctgcgga gcaaggccgt cgcgaacgag
      961 tggcggaggg tgtgcggtgt ggcgggcttc gtgatgcctg cttgttctac ggcacgtttg
     1021 aaggcgcgct gaaaggtctg gtcatacatg tgatggcgac gcacgacacc gctccgtgga
     1081 tcggtcgaat gcgtgtgctg cgcaaaaacc cagaaccacg gccaggaatg cccggcgcgc
     1141 ggatacttcc gctcaagggc gtcgggaagc gcaacgccgc tgcggccctc ggcctggtcc
     1201 ttcagccacc atgcccgtgc acgcgacagc tgctcgcgca ggctgggtgc caagctctcg
     1261 ggtaacatca aggcccgatc cttggagccc ttgccctccc gcacgatgat cgtgccgtga
     1321 tcgaaatcca gatccttgac ccgcagttgc aaaccctcac tgatccgcat gcccgttcca
     1381 tacagaagct gggcgaacaa acgatgctcg ccttccagaa aaccgaggat gcgaaccact
     1441 tcatccgggg tcagcaccac cggcaagcgc cgcgacggcc gaggtcttcc gatctcctga
     1501 agccagggca gatccgtgca cagcaccttg ccgtagaaga acagcaaggc cgccaatgcc
     1561 tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg
     1621 acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga
     1681 acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca
     1741 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     1801 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     1861 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     1921 ggcagtcgcc ctaaaacaaa gttagccatt aagggagtta aattgaaaat atcattgatt
     1981 tctgcagtgt cagaaaatgg cgtaatcggt agtggtcctg atatcccgtg gtcagtaaaa
     2041 ggtgagcaac tactctttaa agcgctcaca tataatcaat ggctccttgt cggaagaaaa
     2101 acatttgact ctatgggtgt tcttccaaat cgcaaatatg cagtagtgtc aaagaacgga
     2161 atttcaagct caaatgaaaa cgtcctagtt tttccttcaa tagaaaatgc tttgaaagag
     2221 ctatcaaaag ttacagatca tgtatatgtc tctggcgggg gtcaaatcta taatagcctt
     2281 attgaaaaag cagatataat tcatttgtct actgttcacg ttgaagtcga aggtgatatc
     2341 aaattcccta taatgcctga gaatttcaat ttggtttttg aacagttttt tatgtctaat
     2401 ataaattata cataccagat ttggaaaaaa ggctaacaat gcgttgcagc accagtcgct
     2461 tcgctccttg gacagctttt aagtcgcgtc tttgtggttt tgctgcgcaa aagtattcca
     2521 caaagccgca acttaaaagc tgccgctgaa cttaacgtta ggcatcatgg gtgaattttt
     2581 ccctgcacaa gttttcaagc agctgtccca cgctcgcgcg gtgatcgagc gccatctggc
     2641 tgcgacactg gacacaatcc acctgttcgg atctgcgatc gatggagggc tgaagccgga
     2701 cagcgacata gacttgctcg tgaccgtcag cgccgcacct aacgattcgc tccggcaggc
     2761 gctaatgctc gatttgctga aagtctcatc accgccaggc gatggcggaa catggcgacc
     2821 gctggagcta actgttgtcg ctcgaagcga agtagtgcct tggcgctatc cggcgcggcg
     2881 tgagcttcag ttcggtgagt ggctccgcca cgacatcctt tccggaacgt tcgagcctgc
     2941 cgttctggat cacgatcttg cgattttgct gaccaaggcg aggcaacaca gccttgcgct
     3001 tctaggccca tccgcagcca cgtttttcga gccggtgccg aaggagcatt tctccaaggc
     3061 gcttttcgac actattgccc agtggaatgc agagtcggat tggaagggtg acgagcggaa
     3121 cgtcgttctt gctcttgctc gcatttggta cagcgcttca actggtctca ttgctcctaa
     3181 ggacgttgct gccgcatggg tatcggagcg tttgcctgcc gagcatcggc ccctcatctg
     3241 caaggcacgc gcggcgtacc tgggtagcga ggacgacgac ctagcaatgc gcgtcgaaga
     3301 gacggccgcg ttcgttcgat atgccaaagc aacgattgag agaatcttgc gttgagcggc
     3361 atgtgcgaaa agtgcatcga cccgcgccga gggcatctga tgcctaactc ggcgttcaag
     3421 cggacgggct gcgcccgccg ctcaactatg cgttagatgc actaagcaca taattgctca
     3481 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta
     3541 cacaaattgg gagatatatc atgaaaggct ggctttttct tgttatcgca atagttggcg
     3601 aagtaatcgc aacatccgca ttaaaatcta gcgagggctt tactaagctt gccccttccg
     3661 ccgttgtcat aatcggttat ggcatcgcat tttattttct ttctctggtt ctgaaatcca
     3721 tccctgtcgg tgttgcttat gcagtctggt cgggactcgg cgtcgtcata attacagcca
     3781 ttgcctggtt gcttcatggg caaaagcttg atgcgtgggg ctttgtaggt atggggctca
     3841 taattgctgc ctttttgctc gcccgatccc catcgtggaa gtcgctgcgg aggccgacgc
     3901 catggtgacg gtgttcggca ttctgaatct caccgaggac tccttcttcg atgagagccg
     3961 gcggctagac cccgccggcg ctgtcaccgc ggcgatcgaa atgctgcgag tcggatcaga
     4021 cgtcgtggat gtcggaccgg ccgccagcca tccggacgcg aggcctgtat cgccggccga
     4081 tgagatcaga cgtattgcgc cgctcttaga cgccctgtcc gatcagatgc accgtgtttc
     4141 aatcgacagc ttccaaccgg aaacccagcg ctatgcgctc aagcgcggcg tgggctacct
     4201 gaacgatatc caaggatttc ctgaccctgc gctctatccc gatattgctg aggcggactg
     4261 caggctggtg gttatgcact cagcgcagcg ggatggcatc gccacccgca ccggtcacct
     4321 tcgacccgaa gacgcgctcg acgagattgt gcggttcttc gaggcgcggg tttccgcctt
     4381 gcgacggagc ggggtcgctg ccgaccggct catcctcgat ccggggatgg gatttttctt
     4441 gagccccgca ccggaaacat cgctgcacgt gctgtcgaac cttcaaaagc tgaagtcggc
     4501 gttggggctt ccgctattgg tctcggtgtc gcggaaatcc ttcttgggcg ccaccgttgg
     4561 ccttcctgta aaggatctgg gtccagcgag ccttgcggcg gaacttcacg cgatcggcaa
     4621 tggcgctgac tacgtccgca cccacgcgcc tggagatctg cgaagcgcaa tcaccttctc
     4681 ggaaaccctc gcgaaatttc gcagtcgcga cgccagagac cgagggttag atcatgccta
     4741 gcattcacct tccggccgcc cgctagcgga ccctggtcag gttccgcgaa ggtgggcgca
     4801 gacatgctgg gctcgtcagg atcaaactgc actatgaggc ggcggttcat accgcgccag
     4861 gggagcgaat ggacagcgag gagcctccga acgttcgggt cgcctgctcg ggtgatatcg
     4921 acgaggttgt gcggctgatg cacgacgctg cggcgtggat gtccgccaag ggaacgcccg
     4981 cctgggacgt cgcgcggatc gaccggacat tcgcggagac cttcgtcctg agatccgagc
     5041 tcctagggat cgcctcagaa aacggaaaat aaagcacgct aagccgtaag taagcgtgct
     5101 cctgtgaaag ccacagctaa aactgcgtag tacacataga ggtatttttt tctagattta
     5161 tatcgaattt cgttatcgaa tttcgcttat tattgatcaa ctcgttgcag atcatgaagg
     5221 cgaaagtatg acgaataacc ctaccgatga cagcagacca tggtcggtct ttcttatttt
     5281 tctgcggctt ggattgacat cttttggcgg ccccattgcg cacttgggct acttccgcgc
     5341 cgaatttgtc acacggcggc gctggctctc cgaacggagc tatgctgact tggtcgcgct
     5401 ttgtcagttc ttgccagggc ctgcaagcag ccaggtcggc atagcggtag gactgtctcg
     5461 ggctggatac agcggggcgc tggctgcttg ggctggcttc acgctgccgt ctgccatagc
     5521 cttgatcctt tttgcgctcg gcatctccag ctatggcgat tacgtctcgc agggcgcgtt
     5581 gcatggctta aaagtggtgg ctgtggccgt ggtcgctcaa gcagtatggg gcatggcgcg
     5641 taacctatgc acggatgggc tgcgagtcac catcatggca attgctacct gcgtcgtttt
     5701 acttgtgccg tccgcgtggg gacaggttgg cgtgattgct atcgcaggca tcgcaggccg
     5761 gttattgttc aagccagcga aagttgttga gcatgacccc ctacctatca cggtcagtca
     5821 ccgggccggc gtgctttggc tctcgctgtt ctttgtcttg ctgattggcc tgccggtgtt
     5881 ggccgaactg atgccaagtc aaaccatggc aatggtggat tccttctatc gtgtcggatc
     5941 actggtgttc ggcggtggtc acgttgtgct gccattactg caagccgaag tggtgccctc
     6001 cggctgggtc aacaatgaat cctttctcgc ggggtacggg gcagctcaag cggtgcccgg
     6061 ccctttgttc acgttcgccg cgtttcttgg tgcctcgatg aacaccgccc cgtcgggctg
     6121 gatcggcggc attgtgtgtc tgctggctat cttcgcgccc tcgttcttgc tggtcgtcgg
     6181 atcaatgcca ttttgggagc gtttgcgccg caatacaggc atccaagctg cgctggccgg
     6241 gatcaatgcc gctgtagtcg gcttgctgct ggccgcgctg tatcagcctg tatggactag
     6301 cgccatcttt cagccgcaag acttcggctt ggcattagtt gcccttgtcg cacttatgtt
     6361 ctggaagctc ccgccgtggc tggttgtcct cggtagcggt gcagctgggt ggctgttgag
     6421 cgtcgctctg tgaggcacaa aaaatgactg acaaagacct ctacggcggt ttgatccgcc
     6481 tgcacatcct tcaccatgca gccgaggaac ctgtctttgg gctggggatc atcgaagagc
     6541 tacgccgaca cggctacgag atgagcgctg gcaccgtgta cccgatgctg cacggcctgg
     6601 aaaaaaaagg ctatctgacc tcacgccacg aacgcaccgg gcgacgtgaa cggcgtgttt
     6661 atgacatcac tgaacaaggc agaactgcac ttgctgatgc gaaaacaaag gtcaaggagc
     6721 tgttcggaga gttggtagaa ggtggttgag tttttgcttc tatgtcaggt tgagctatac
     6781 cctaactgga ttgtcatttt cagaagacga ctgcaccagt tgattgggcg taatggctgt
     6841 tgtgcagcca gctcctgaca gttcaatatc agaagtgatc tgcaccaatc tcgactatgc
     6901 tcaatactcg tgtgggctct gttgcaaaaa tcgtgaagct tgagcatgct tggcggagat
     6961 tggacggacg gaacgatgac ggatttcaag tggcgccatt tccagggtga tgtgatcctg
     7021 tgggcggtgc gctggtattg tcgctatccg atcagctatc gcgaccttga ggaaatgctg
     7081 gcggaacgcg gcatttcggt cgaccatacg acgatctatc gctgggtcca gtgctacgcc
     7141 ccggagatgg agaagcggct gcgctggttc tggcggcgtg gctttgatcc gagctggcgc
     7201 ctggatgaaa cctacgtcaa ggtgcggggc aagtggacct acctgtaccg ggcagtcgac
     7261 aagcggggcg acacgatcga tttctacctg tcgccgaccc gcagcgccaa ggcagcgaag
     7321 cggttcctgg gcaaggccct gcgaggcctg aagcactggg aaaagcctgc cacgctcaat
     7381 accgacaaag cgccgagcta tggtgcagcg atcaccgaat tgaagcgcga aggaaagctg
     7441 gaccgggaga cggcccaccg gcaggtgaag tatctcaata acgtgatcga ggccgatcac
     7501 ggaaagctca agatactgat caagccggtg cgcggtttca aatcgatccc cacggcctat
     7561 gccacgatca agggattcga agtcatgcga gccctgcgca aaggacaggc tcgcccctgg
     7621 tgcctgcagc ccggcatcag gggcgaggtg cgccttgtgg agagagcttt tggcattggg
     7681 ccctcggcgc tgacggaggc catgggcatg ctcaaccacc atttcgcagc agccgcctga
     7741 tcggcgcaga gcgacagcct acctctgact gccgccaatc tttgcaacag agcccgccgt
     7801 gctagtctgc tcggtgatgg tggagtgaag ccaacccgca atcgggttat gaatctgcat
     7861 cgcgattcgc aatcagctgt ctcttgagca tgtcgaactc ctgcgatgtt atctcgccgt
     7921 gctcgtgctg ctacaccaac ttgtccaact catccgccac accgctcccc accacctgcg
     7981 atggccaggt tgtttgctgg gcgcattggc ggagtatcgt ctcgacccgg gacacccaca
     8041 ccggcacaac cttacgggag tgattcactg tcaaagaatc ggcccggtgc tctgacgcaa
     8101 gtatcgggat ggtcaccatt tgtaagccgt agacctgagt ggtgatcaag acttcgatac
     8161 caccgaccgt accggtacta atcgacgacg gtcgtgttcg tcgcctgccg cagggactct
     8221 gcacacctcc gtttacgcat gtgcctggag gagttggaaa tcgtcgtgtt cgggaaacat
     8281 taaacacagg atggcagcga tctgagccag cacatgatca gctagctcac catccggatc
     8341 gacggcccac tgcatcgtcg cgccagcgat gaccgagtgc aggagcaact cagctgccgc
     8401 aggagcacct gggggcagtc gcttgcggat cccctccacc accgcgcggt tccgctggat
     8461 cgcaagcgtg cgtagctccg gcacctggag ctcgtaccag gagatgagat agttcaccga
     8521 gaagtcgttg cgagtgttca tgctccgaac gagcacctgc aaaaattccc agagcccttg
     8581 cggccctgcg cctatcggta tcgcattcag gtaatgccgc acctgctcga cgccgcgctc
     8641 catcatcctc accagcagcg tatcgcggtt ggtgaagcgc tggattaacg ctgcgcggga
     8701 gagccccacc tcctttgcta ctccgctgag cgtgaactct atgggaccgc aacgcttcag
     8761 cactacggtg gcggcctcga gtacctcgtc atcggacttg agcttggggc ggggcatcag
     8821 tgttcacctt ctgtatgggt tggggcggag gctgtggctg ccgccgccat tgtagcaaat
     8881 tgaagacgga gcgagagtag agccacgagc cccgcaaaca cggccgatac aacgaggcca
     8941 gggagcggac cagcaaggtc gacaaacgcg ccggccgcaa gcataaccat gggcgaggct
     9001 gacagcatca ccgccgagac cgtgccgagt acccggccga gaagttctgg cggcgtgcgg
     9061 ttgtagatgg cagcgttgag aatgggagag actgagccgg tcagcagtcc cacgagcgcg
     9121 cccaacaaca tcagcaccgg cacgcctggc aactgtgaaa gcagaagcga gcccaccgca
     9181 gagccacaaa atgccaccgc cagccagttc tgcgctgata tccgggcgcc gaccgacgca
     9241 tgaatggcaa tgccaaggag accaccagcc cccatcattg aggagaacag cccgagctct
     9301 gctacttggc gtcctgcatc tacaaacagc gcaggcatga tgacgctgcc gttggcgcca
     9361 acgatgccca cgaagatcat cactatacca aagagagggc gcagcagggg ttcgctccag
     9421 agaaaagcga cgccggcgcg catggagaga gtcgccgtcg tggtcatcgt ccgagcggca
     9481 cgcgcgggaa gcacccacgc gccgagcaga cctgcaagga cggagcagaa cgccgtcagc
     9541 ccgagcgttg gcgcagcgcc aagcaggccg attgcggccc ccccaagggc cgggccacct
     9601 agaatcgcga cgttcccgat caccgctttc agtgacgaga cgcgctcaac ggagagcccg
     9661 gcgacgtggc cgagtttggg cagctcactg tcctgcgcgg ccataccggg tgcgtcgaac
     9721 gcggcaccga gcaccacgca agcgatcagc ccagtgttcg agagggcgcc aacggcatcg
     9781 agcagtggga tgctcgccat ggccacgccg cccaccacac ccgagatcaa tgcgacgggc
     9841 gcgcgcccga accgatcgac gaggccacca ccaacccacg cgccgatgat ggtcgcgatg
     9901 acgctgctag cggccgtggc gcccgcccag gccgcgctct ttgtatgaga caggacgaac
     9961 catggaagcg cgagggccgc caccgcgttg ccgatccgga agagaaaggt cgccgcgaac
    10021 agcgtcgcga gcgggctata tcgacgttcg ctcattccgc tgcggcgagc tgcgccttcg
    10081 ccgcagcgag gtactcttcg ttacccgagt cgagggcgaa gagtgcgtag gtgaccgccc
    10141 cgaacgcaag gcgctccgcg atgtggtggg cgagccgcgg ccacacccgg ccaccggccg
    10201 cttcatacgt gaggaggagc ttcgcgagcc cctcttcacc aaagaccata aggtgcgcgg
    10261 ccatgtcgat ggcagggtca tcaacgcggg cctcgctcca gtcgatcatc ccgctgacgc
    10321 gctccgtgtt gtcgatgagc acatggccca cgtagagatc gccatgcacc accacggaga
    10381 aatctggcca cgacgaatcg tcgtcgagcc agcgctgcca ccggtggagg cgcttgtcgt
    10441 tcaccacgaa ctcgcgtcgg acgcggtcaa cgtcgtcggc caccttctga cgggcctgcg
    10501 tcggtgtacg gatgagcatc cccgcatcca cggcggcgga aatggggacg gcatgcaggg
    10561 cggcgagcgc ggtcgcgaag ctctccgcga agacctccga gtcctgcggc acgacccagt
    10621 cgggcgtgga cgaaccaggc tggatgacca tcgcagtcga gtcttcgagc atgggatagg
    10681 caacgagctc ggcgttggcc acgcgccagt ccggcaccgc gaacggcagg cgattcttga
    10741 gcattgccag cacccgcgcc tctggttcga ccttcgcgct tacctcggct cggcgcggga
    10801 tgcgcagcac ccaccgacgt ccatcgtcga cggtggcgat cacgatccta tagtcgagcc
    10861 caagctcatt gacagtcagc gggccatgga gcttgagccc atgtcgggct gcaagtgcgt
    10921 acagttggga ggtatcggcg gtcgtgacta cggtcatgat tcactcctga gggcttgacg
    10981 ggtttagcca cctaaatgta acagtcacgt cggttatatt caatc
//
LOCUS       DAZXUK010000127        11011 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZXUK010000127 DAZXUK010000000
VERSION     DAZXUK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11011
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.127"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..317)
                     /gene="chbB"
                     /locus_tag="ACH3L8_002562"
     CDS             complement(<1..317)
                     /gene="chbB"
                     /locus_tag="ACH3L8_002562"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFT4085626.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAA"
     gene            complement(616..954)
                     /gene="osmE"
                     /locus_tag="ACH3L8_002563"
     CDS             complement(616..954)
                     /gene="osmE"
                     /locus_tag="ACH3L8_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFT4085627.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1156..1983
                     /gene="nadE"
                     /locus_tag="ACH3L8_002564"
     CDS             1156..1983
                     /gene="nadE"
                     /locus_tag="ACH3L8_002564"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFT4085628.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            2213..3100
                     /gene="cho"
                     /locus_tag="ACH3L8_002565"
     CDS             2213..3100
                     /gene="cho"
                     /locus_tag="ACH3L8_002565"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFT4085629.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3060..3635)
                     /gene="ves"
                     /locus_tag="ACH3L8_002566"
     CDS             complement(3060..3635)
                     /gene="ves"
                     /locus_tag="ACH3L8_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFT4085630.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3838..4323)
                     /gene="spy"
                     /locus_tag="ACH3L8_002567"
     CDS             complement(3838..4323)
                     /gene="spy"
                     /locus_tag="ACH3L8_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFT4085631.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4653..5621)
                     /gene="astE"
                     /locus_tag="ACH3L8_002568"
     CDS             complement(4653..5621)
                     /gene="astE"
                     /locus_tag="ACH3L8_002568"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFT4085632.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5614..6947)
                     /gene="astB"
                     /locus_tag="ACH3L8_002569"
                     /pseudo
     CDS             complement(5614..6947)
                     /gene="astB"
                     /locus_tag="ACH3L8_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(6944..8422)
                     /gene="astD"
                     /locus_tag="ACH3L8_002570"
     CDS             complement(6944..8422)
                     /gene="astD"
                     /locus_tag="ACH3L8_002570"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFT4085633.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8419..9453)
                     /gene="astA"
                     /locus_tag="ACH3L8_002571"
     CDS             complement(8419..9453)
                     /gene="astA"
                     /locus_tag="ACH3L8_002571"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFT4085634.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9450..10670)
                     /gene="astC"
                     /locus_tag="ACH3L8_002572"
                     /pseudo
     CDS             complement(9450..10670)
                     /gene="astC"
                     /locus_tag="ACH3L8_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
BASE COUNT         2680 a         3003 c         2717 g         2611 t
ORIGIN      
        1 tttgctgcgg cttttttaat cgctgcaacc gcagccttaa gcacgcctaa accatcgact
       61 ttgccataaa gcagcgagtc aattacttca accggtttgt tgggtaacaa acgctggatt
      121 tcgggcaaca tataagcaat ctgcggccct aataacacga catcggcatt ctgacctttt
      181 tcaccagcca gtgtttccgg aaatgcttca ataatgaccg gaacttcata tttttctgcc
      241 tgtgcgcgca tttttgatac cagtaaagag atagacatgc ccgcagaaca aaacagataa
      301 atgtgtttct tttccataaa actgccctca tcgacgatta tctgtcagcc agacactccg
      361 caagccttga cctgcttcca tgctctgggt aacttgcgaa accaacatga ctttttttgt
      421 ggctgaaagg agtatacggt atagacctga gcaatagtat ttctttgacc ttataaattg
      481 tttctccttg acctgtggtt atgaccctct tcacatttcg ggcaaataat tcgcgaagat
      541 aattaagaac tcaggccaaa aaaaaccggc gcaatggccg gtttccgttg ttactcaagc
      601 tttcagacga attgattact tcgcagcctg tggatcagtg tcgtattcag cacaggtctg
      661 ataaccggag ttgatgacat gtccggtatc atctaacgcg acaaagtagg tttctgcttt
      721 accatcacgt tgacccagga tgtaggtctg gcaagtaccg cgagcatgga tcatgctcac
      781 ttcagacgaa ggtttacccg caatttgtgc aacctgcgcc cggctcatgc cttttttgac
      841 gtctttcacc acaggctgta caaactggtc tttggtacga tcataagctg tacaacccgc
      901 cagcatggtt aataccgccg ctgcactcag aattcctgcc atattcttgt tcatattccg
      961 tcctcttgtt tatcagcgtg ttagataagc ctggaataca ttgggcgctt tttcaagccc
     1021 gtgaacgaaa cggctccgct ttcagaggat tcctgtatga cgttttaacc accattcagc
     1081 ccgctgtcgc ttgtcgtttc agtagcaacg ggttagcttt aaggaagttt tgtcttttct
     1141 gtctggaggg gttcaatgac attgcaacaa caaataataa aggcgctggg cgcaaaaccg
     1201 cagattaatg ctgaagagga aattcgtcgt agtgtcgatt ttctgaaaag ctacctgcaa
     1261 acttatccgt tcattaaatc actggtgctc gggatcagcg gcggtcagga ctccacgctt
     1321 gccggaaagc tgtgccagat ggcgattaat gagctgcgcc aggaaactcg caacgaatca
     1381 ctgcaattta ttgccgtacg cctgccctat ggtgttcagg ctgacgaaca agattgccag
     1441 gatgccattg cctttattca accggatcgc gtattaaccg ttaatatcaa gggcgcggta
     1501 ttggctagcg agcaggcatt gcgggaagca ggcattgaac tgagcgattt tgtccgtggc
     1561 aatgaaaaag cgcgtgagcg gatgaaagca caatatagca ttgcgggtat gaccagcggt
     1621 gtcgtggtgg gcaccgatca tgcagcagaa gccattaccg gattcttcac taaatatggt
     1681 gacggcggta cggatattaa tccgctgtat cgtctcaaca aacgccaggg taaacagtta
     1741 ctgacggcat taggttgccc ggaacacctt tataagaaag ccccaactgc cgatcttgaa
     1801 gatgatcgcc cttctctgcc agatgaagtg gcactcggcg tgacctatga caatatcgac
     1861 gactatctgg aagggaaaaa cctacctgaa caggtcgcca gaacaataga gaactggtat
     1921 ctgaaaaccg aacataaacg ccgtccgcca attaacgttt tcgatgattt ctggaaaaag
     1981 taataatttg cacctattgg attgtgcgaa aaagagtaat ttgttcacgc cggatgcggc
     2041 gtgaacgcct tattctacct acaaaactat gcaaattcaa tacattgcag gagtcgaata
     2101 ggcctgacag gcgtagcacg tcagacggtg taacctttgt catcaacccg cctctttttt
     2161 aatcgcctcc cgcctgttac actggataga taaccagcat tcggagtcaa cagtggtacg
     2221 gcgtttaact tctccgcggc tcgaatttga agctgcggca atttatgaat atcccgaaca
     2281 tttacattca ttccttaatg acttacccac ccgaccaggg gtgtatctgt ttcatggcga
     2341 aagtgacacc atgccgctct atatcggcaa aagcgttaat atccgcagtc gggtgctttc
     2401 tcatttacgc accccggatg aagccgccat gctacggcaa tcccggcgaa ttagctggat
     2461 ctgcaccgca ggcgaaatcg gcgctctgct ccttgaagcg cgattaatca aagaacaaca
     2521 gccgctgttt aataaacggt tgcgccgcaa tcgccagctc tgtgccctgc aattaaatga
     2581 aaagcgcgtc gatgtggtgt atgccaaaga ggtggatttt tcacgagccc ccaacctgtt
     2641 tggcctgttt gccaataggc gcgcagcttt gcaagcattg cagagcatcg ctgatgaaca
     2701 aaaactttgt tatggcctgt tgggactgga accgttaagt cgcggtcgtg catgttttcg
     2761 ttcagcgcta aaacgttgcg ccggagcatg ctgcggtaaa gagagccatg aggaacatgc
     2821 gctacgcttg cgccagtctc tggagcgttt gcgagtggtg tgttggcctt ggcaaggtgc
     2881 ggtggcactg aaagaacagc acccggaaat gactcaatat catattattc aaaactggct
     2941 gtggctgggg gcggttaatt cgctggaaga agcgacaacg ttaattcgga cacccgccgg
     3001 gtttgatcac gacggttata aaattctttg taagccgctg ctttccggta actatgaaat
     3061 tactgaactt gatccggcga atgaccagcg agccagttga tttcactgaa caacagttta
     3121 ccttgcggtt gtaaaagccg cagggtgtgc ctgccatcga accagcacac gccctggtcg
     3181 gtagtgagca gtttgtcgcc cagttgccac gccccgttaa tcacaaacac caccccgccg
     3241 cgtgagccaa aggtagtaaa cgtacgttcg gcaattctga ctttcgcctt acagacatcc
     3301 aggcgcgtca taatattgaa atccatcgac atctgccctg ccgtcagttt cgcttttacc
     3361 acctggtccg ccgtaaaggc aaaaggctgt aacggtttta aggtatggtt aaagcggtct
     3421 gcgctttcaa ggagcatctc gccgccttcc agcaacgtca ctatcctttc catgccggga
     3481 aacaaagaaa actccccatt cgccgcgatg gatgcaatgc tggcacgcca gtaaaaatca
     3541 cgttttgccg gtgggaaagt gcaaatttcg cgcgtttcac cggcagcgtt tcgccacaga
     3601 ttcaccgaca ttttacgcat atcaaagtat tccatactcg ctcccggccc gttcttttca
     3661 ttgttatcgt tcaggtaacc gtggatggtt atcggcaact cgggtgattt acgttagtgg
     3721 tgatcaggct ttttttacaa ctgtttgaga agagaaaaga aaaccgccga tcctgtccac
     3781 cgcattactg caaggtagtg gacaagaccg gcggtcttaa gttttttggc tgaaagatta
     3841 ttcagcagtt gcaggcattt taccttttgc cgctggacgt tctgtcagac gcttctcaaa
     3901 attagcatta aattgctttt tctgttccgg cgtcaggatg ttgtaaattt tgttctgggt
     3961 ttccatgtgc gccagcatgt tagctttgcg ctgttcttcc atttttgcga tctgcgcttc
     4021 agcttttgct ttatcgaagg tatcgctggc aatgatgtca tgcattgcgc ggcgttcttc
     4081 cagcggcgga cgtttcatct ggtcacgctg gcctttcatg atttcgtgga tctgctgttt
     4141 ctgcgcgtcg gtcaggttca ggtctttgaa catcatgtcc tgatgcggac cgaacttgcc
     4201 tttgtggtgc atcatcggct tcgcgtcagc cggtgctgcg gtagtggtgt ctgcggcatg
     4261 ggccaggtta gccgcgccaa gagccagggt agaggcaaca aacagtgcag ttaatttacg
     4321 catattctat atccttcctt tcagttattt attacggctt tcttaagtag cgtgccgtgt
     4381 tgacgagatt aactttactg actttagcgt caattaatca gagcaacggt aaaacaatga
     4441 aagtgtaaaa aacacttttg cgccaattat ggagaaaaaa agaaaatttg atggagagtg
     4501 atgagagaat attacaacac gatgattttg cagagattat gaagaaccat accggatgac
     4561 tggtgataaa taaagcaaat aaccaggatt aatctgtatt aatttataag aaagcaactt
     4621 aatacccgca gaatgatttc tgcgggtaag tattagctta ttttttcgag cattaatccc
     4681 gcgcgtaatc ccaacgctac caacggatta gggaataaca catactctac atcatgggtt
     4741 acggtaaaac gttcctctcc gtcctgcgcc agcaatgttc ctttctcaaa cggcataaaa
     4801 ttcagcgtgt cacttgccat atgcatttcg aaggacggcg agtggcgagt aatttgcgaa
     4861 accacccgat aacggagcgg cggtgttctc acgataccga cactctcacc agatagcagc
     4921 gcagcaattg cgctggcagt tactgcaaac tggcgaagat cgttttgccc aaagggcaac
     4981 gctttgccaa gttccaacgt acaggccagc gcgccaaaat gtctgacgct gaaatgggta
     5041 aacgtaccac caggctcctg atggaacacc agcgcctcca gccccgccgc acccagccac
     5101 gtcagaaatt tctcgtccca gggaatgtcg cgttgcggta atacaccgaa ctgcggatgc
     5161 aaggagccac gaattgcggt atgtagatca aggtgccaac gcacagattc tttgccctgg
     5221 tcataaaaat cttccaggca ctgttccagt tcgcgcgccc gacaggtttc tccgctttca
     5281 gcaaatagct gccaacgacc gccaaacatt cggttcatat cgctatggca ataacgtttc
     5341 ccttgcttca gcgcaggagg attcccgagg atcaccagca accgccaacg taacgggatc
     5401 tcgccgtgag atatcgcgcc aagcaacgcg tccagcattt ccacaggtgc cgtctcatta
     5461 ccgtggatcc ccgcagaaat caccagcgcg ccttgcggtg gcgttaatgg cgtcagttcc
     5521 agcacaccat cgcccagcca gcgccagcga acgccgttga tttctcgctc ggtgataacc
     5581 ggttttttac ccgttaaggt cagagcaaga aaattatcca ttgccccctc cctcgcgctg
     5641 gaacggataa accgaaccga gattcagtaa ttggctcaat acatccagtg cttcccgccc
     5701 ttcgcgcagc aattgcgggt cggccagatc ggcagcagta agacgatcgc ggtagtaacg
     5761 atccacccag tcattgagcg cattaaacaa ggtatcgttc atcatcaccg ccggattcac
     5821 cgcccggcgt tcttcttctg tcaataccac ccgcaaccgc aggcacgccg ggccgccgcc
     5881 attcgccatg ctttcacgta agtcaaagac ttttagttcg ctaatcgggt tgtcagcggc
     5941 aaggagttca ttgagataac cccatactcc ggcgtgttcc cgacactcct gaggcagcac
     6001 caacatcatg gaaccatcat cgcggctcaa cagttggctg ttaaacagat aggtagacac
     6061 cgcatccgac acggaaacct gagttgccgg aacttctatc gccataaaac cgttcacccg
     6121 cgtgccagta actgtgactg gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg
     6181 ttactcacgg caatcacgtc attatgaaaa acgctctggt cgataacgtc cgggttttgc
     6241 tgggcgaaaa tcacctgttg gggattcacc tgattaagcc ttgccaccgc ctcgctggct
     6301 tcgcgagtct gtcgcgccgg ataacgggaa ggccgggtat cattgccttc ttctcgcccg
     6361 tagacaaaaa gtagcatacc cggttcaccg taatgaccgc cgagacgatt gtggtttgcc
     6421 gccccctcat caccgagcaa cgctacctgc ggcaacgccg aatggacgct aaatttctct
     6481 tcgtccttaa aaatcgcttt taacagcgat tcagtgacgg gcgcttccag cgaacggtga
     6541 aatttattgt tcaggttggc aacggtgaga tgcactttgc catccagcgt atcggcagat
     6601 ggcgcgatcg ttgccgcatt ggctacccac attggcgaag cggaactgac gctggaaagc
     6661 cagtgcggtg cctggcgtgc aactttttcc agtacctgct catcgctacc actgaatccc
     6721 aactgacgca gcaccggaat aaacggacgc tcgtgcggcg gaatcacggc ctgggggaat
     6781 cccgcatcgg caagggcttt cattttcagt aagccctgtt ttgccgccag tcgcgggtta
     6841 gacacctgaa aacggtgacg ggtagaggct tcattaccaa acgacaggcc cgcgtaatga
     6901 tgcgtcagcc ctaccagccc gtcgaaatta acttcccagg cgttcatcgc accacctcat
     6961 cggaaaaatc cagcccgggg ttaagcgtgg cgggcaatgt taacgagtcc gactccaggc
     7021 tcgccatagg ccatgcgcag taatctgcgg cataccaggc gctggggcga tggttaccgg
     7081 aagcaccaat gccgccgaat ggggcggtac tggcagcacc agtaagcggt ttgttccagt
     7141 taacaatccc cgcccgcgcc tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa
     7201 ccagaccgca aaagagtccg aagcgagtgt tgttcgccat ccgaatcgcc tcatcaaaag
     7261 tatcataacg ccagacgcgc aaaaacggcc cgaacacctc ttcatccggc acaccgccaa
     7321 cgcccgtcat ttcaatgatc cctggtgtca gcaacgatgt ccctgcttgt aataagcgcg
     7381 gcgcaagcag ggttcgtccg cccatcgctt ccagttgctg ccaggcagta accacctgct
     7441 gtgcggcctg ttcagaaatc agcccgccaa taaacggctg cggttcgtca tcccagttgc
     7501 ccggcgttaa tcgctggctg acggcaacca gactagcaag aaacgcatcg ccctgcgctc
     7561 cgcttttgag caataaacgg cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg
     7621 actgaatggt cagatggaca gccgcgtcga tatccgccac ctcatcgata attagcggat
     7681 tattaccgcc catctcaagg gcgagaattt tctccggctg accggagagc tggcgatgca
     7741 gctggtagcc ggtattggcg ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac
     7801 tcagcgcctg acccgtttca cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc
     7861 cagcctgctg ccataaacgc attaccgctt cgccactcca cggtgtcagt tcgctgggtt
     7921 taaagataat ggtgttacct gccagcaatg ccggaacgat atgtccgttc ggcaaatgac
     7981 cagggaaatt atacggccca aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg
     8041 ccgcaccgtc cggcatttca ctacgctgct cgccggtacg aacgtgatac gccttaattg
     8101 atatcgcgat tttattgatc atcgccgtca cttcggttgc cgcttcccag cgcggcttac
     8161 ccgtttctct ggcaataatc gcggttaatt cggctttatt gctttccagc aatccggcaa
     8221 agcgttcgac aacgacctga cgttcagcca atgagagccg cgcccagcgc ggaaacgccg
     8281 cacgggctgc ccgacaagcc tgttcgacct gagcggcatc ggcatcattg ccttgccata
     8341 acacctcgcc cgataccgga ttacgcttca cacgcgatgc gccctggccc gttatccagt
     8401 caccgttaat ccataaagtc atgctgtttt ctcctctgcg cacaggcgca ccagacgaac
     8461 gcgatcccct gcgtggcatt tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc
     8521 ggttgccgga tcggtacgcg ccagcaccac gcggaaatgg tgataatttt cattggcgac
     8581 caggcaggcc ggaaaatcgc cctgcgcagg ctgcccttct gccacttcca ccagccgact
     8641 tttacggatg gcgcgcacgc ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat
     8701 gtcgatatag ttgcggtaac gaaaaccttc tttctccagc accgcgcggg caggcgcggt
     8761 ttgcggatgt acctgaccga tgacgtcctg ggcttcctgg gataaaaagt gggtatagat
     8821 cggatgtttc ggcatcagtt ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc
     8881 ggcgcggcta aaatccatcg aaaagaagcg tttaccgagg ctttgccaga acggtgaata
     8941 gccgtgttcg tcaatcaccc cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg
     9001 aaaagccgcc ataaacataa agcgcgattt cgatagcaaa tagccgttgc cctctttgcg
     9061 ccagtccggg tcgagaaaca gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag
     9121 aaacagcgtc ggcaatgcgt tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc
     9181 gacgcgatag ttgtaccagg gatcgttcag cccaaccgcc acctcaatag cgcaaatccc
     9241 cgccacggtg cctgtctcgc tatcttccag cacgaacaca tagccctgct cgcttttggg
     9301 cagttcacct tgccaggttt tgattgccct ttcgatacgc gccgaaagcg tggcttcatt
     9361 ggcgggaagc gacgtcaggc cgccgcccgt tttgctggca agctgcatca gcgccgagac
     9421 atctgagcgc tcaacgggac ggatgaccat catgatgaac ctcggctaac aaagtgttcg
     9481 caagcagctg caaagcgatc cagtccggtc gtcacctctt cttcgctgac attgagcgca
     9541 ggcgcaaaac gcaccacgtt gccacccgca atcagtacca tcacgcctgc tttcgccgct
     9601 tccagagaga tctgtttcgc ttgcccggcg taatcggcat tcagtacaca gccaatcaac
     9661 aaacctaagc cgcgaacttc actgaacaaa ccatagcggt gattaatagt attaagacgc
     9721 tcaacaaacc agtcgtgacg ctgtttaacg ccattaagca tctctggtgt gttgatgagc
     9781 tccagcactt tgcctgctac cgccgaggcc agcggattac cgccataggt ggtgccatga
     9841 gtgccaacgg tcatcacgcg ggcgcactct tcggttgcca acaacgcacc gaccgggaaa
     9901 ccgccgccca gcgctttggc ggtggttaac agatcgggcg tcacgccgta gtgcatatag
     9961 gcatacagct caccagtgcg cccgacgccg gtttgtacct catcaaaaat cagcaaggca
    10021 ttgtggcggt cacacaattc acgcagacct tgtaaaaacg cgttgctggc tggcaccaca
    10081 ccgccttcgc cctagatggg ctcgacaatc accgcacagg tggcgtcgtc aatcagcgcg
    10141 ctggcagagt taatatcgtt atatgcagca tgacgaatat ccgccggcag tggcgcaaaa
    10201 tcctgtgaat aggctggctg cccacccgca ctgacagtaa acagcgtgcg accatgaaac
    10261 gcatttttga acgccacgat gccactctta tggctgccgt agcggtcgtg agcgaattta
    10321 cgcgccagtt ttagcgccgc ttcgttggct tccgcaccgg agttacaaaa gaagacgcga
    10381 tcggcaaacg tggcgtcgat caattttttc gccagtcgca gtaccggctc gttggtgtaa
    10441 ccgttgccgg tatgccagaa cttactcgcc tgttcgttca gcgcttcacg cagttccgga
    10501 tgcgcatggc ccagcgcgtt caccgcaatg ccacccgcga agtcgatata ctctttcccc
    10561 tgctgatccc acaagcgcga accttcgcca cgtaccggta taaagggtgc cggagcgtaa
    10621 acaggtatca tccattcatc aaagttttca cgcgtaattg gctgagacat agcgacctct
    10681 acagtaaata attcgttatt tgtatgttaa caataagtaa tgtttgcgct gtaaatgtag
    10741 attgcagggt tcgtgccagc cagtgataaa agtgcataaa cggcggaggc taactggaaa
    10801 tcaaggagtt ataaccaaac catatgcatt taaagtgcat ataaagtgaa tacgtttgcg
    10861 atgtgggtga ataaaaagaa taaaaaacgc aatgttatgc agaagtaaaa tataattcta
    10921 gaattgtgat cattgacgaa atttactgga aattactgcg ccattctgac gcagcgcgca
    10981 ccaaaagcgg gcattttttg cgccatcgtt g
//
LOCUS       DAZXUK010000128        10970 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZXUK010000128 DAZXUK010000000
VERSION     DAZXUK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10970
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.128"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            88..849
                     /gene="adiY"
                     /locus_tag="ACH3L8_002573"
     CDS             88..849
                     /gene="adiY"
                     /locus_tag="ACH3L8_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFT4085635.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            986..2323
                     /gene="adiC"
                     /locus_tag="ACH3L8_002574"
     CDS             986..2323
                     /gene="adiC"
                     /locus_tag="ACH3L8_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFT4085636.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2427..4070
                     /gene="eptA"
                     /locus_tag="ACH3L8_002575"
     CDS             2427..4070
                     /gene="eptA"
                     /locus_tag="ACH3L8_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFT4085637.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            4067..4735
                     /gene="basR"
                     /locus_tag="ACH3L8_002576"
     CDS             4067..4735
                     /gene="basR"
                     /locus_tag="ACH3L8_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFT4085638.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4736..5836
                     /gene="pmrB"
                     /locus_tag="ACH3L8_002577"
     CDS             4736..5836
                     /gene="pmrB"
                     /locus_tag="ACH3L8_002577"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFT4085639.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5812..5901)
                     /gene="pmrR"
                     /locus_tag="ACH3L8_002578"
     CDS             complement(5812..5901)
                     /gene="pmrR"
                     /locus_tag="ACH3L8_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFT4085640.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6013..7515)
                     /gene="proP"
                     /locus_tag="ACH3L8_002579"
     CDS             complement(6013..7515)
                     /gene="proP"
                     /locus_tag="ACH3L8_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFT4085641.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7779..8657)
                     /locus_tag="ACH3L8_002580"
     CDS             complement(7779..8657)
                     /locus_tag="ACH3L8_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFT4085642.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEGLRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(8654..10882)
                     /gene="crfC"
                     /locus_tag="ACH3L8_002581"
     CDS             complement(8654..10882)
                     /gene="crfC"
                     /locus_tag="ACH3L8_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFT4085643.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2742 a         2869 c         2690 g         2669 t
ORIGIN      
        1 tattcatcat gcaattatat aaccatttcc cggtatcgct ttgctttagc gagaactggt
       61 cttttattcg cactcaggag tacatgtatg aggatttgca gcgaccaacc ttgtattgtt
      121 ttattgactg aaaaagatgt ctggataagg gtgaatggga aagaacctat tagccttaaa
      181 gctaaccata tggcgttatt aaattgtgaa aataatatta tcgacgtctc ctctcttaac
      241 aacactttgg ttgctcatat tagtcacgac atcatcaaag attacctccg gtttctgaat
      301 aaagatctct cgcaaatacc agtatggcaa cgtagcgcta cgcccatact caccctgcca
      361 tgcctgacgc cagacgtctt tcgcgttgcc gcgcaacaca gcatgatgcc cgcagaaact
      421 gagtcagaaa aggaacgaac acgcgcatta ttattcacgg tgctatcccg ttttctcgac
      481 agtaaaaaat tcctttcatt aatgatgtat atgttacgta attgtgtaag tgacagcgtt
      541 tatcaaatta ttgaaagcga tattcacaaa gactggaatc ttagtatggt agccagttgt
      601 ttatgtctta gcccaagtct gttaaagaaa aagttgaaaa gcgaaaacac cagttatagc
      661 caaataatca ccacctgccg catgcgttat gccgtaaatg aattaatgat ggacggtaaa
      721 aatatctctc aggtatcaca gtcctgcggc tacaacagta cgtcgtactt tatttctgtc
      781 tttaaagact tctacggtat gacgccgctg cattatgtta gtcagcacag agaacgcact
      841 gtcgcctgat ttttaacctt aacgaagagc tatattaata acggcatcag cgataacccg
      901 gtcgataata attcaactat cgaatgcagg cgtatgatat gacgtaattt attgtcacga
      961 agctcgcctt cgcaggagtt taattatgtc ttcggatgct gatgctcaca aagtgggctt
     1021 aatccccgtc accctgatgg tgtcggggaa tattatgggg tcaggtgttt ttctgttacc
     1081 tgcaaacctg gcctccactg gcgggattgc catttatgga tggttggtga cgattatcgg
     1141 tgcgctgggg ctctcgatgg tatacgccaa aatgtcgttc ctcgacccaa gtcctggtgg
     1201 ttcttacgct tacgcccgcc gctgctttgg cccgtttctc ggttatcaaa ccaacgtcct
     1261 ctactggctg gcctgctgga tcggcaatat cgccatggtg gtcattggcg taggatattt
     1321 aagttacttc ttcccgattc tgaaagatcc attggtatta accatcacct gcgtcgtggt
     1381 gctgtggatc ttcgtcctgc tgaacattgt cggtccgaaa atgatcaccc gcgtgcaggc
     1441 cgttgccact gtgctggcgc tgatccccat cgtcgggatt gccgtatttg gctggttctg
     1501 gttccgtggt gaaacctata tggcggcatg gaacgtcagc ggcctgggca ccttcggtgc
     1561 gattcaaagt acccttaacg ttacgctgtg gtcgttcatc ggtgtggaaa gtgcctccgt
     1621 tgccgcaggt gtggtgaaaa acccgaaacg caatgtccct atcgccacca ttggtggggt
     1681 attgattgcc gccgtttgct atgtgctctc caccaccgcg attatgggga tgatccctaa
     1741 tgccgcactg cgcgtttctg cctcgccatt cggtgatgcc gcacggatgg cgttgggtga
     1801 caccgctggg gccattgttt ccttctgcgc agctgcgggt tgcttaggtt cactgggcgg
     1861 ctggacgttg ctggcgggtc aaacggcgaa agccgctgcc gatgacggac tgttcccacc
     1921 gatttttgcc cgtgtaaata aagcgggtac gccagtggcg gggctgatta tcgtcggtat
     1981 tttgatgacc atcttccagc tcagcagcat ttcaccaaac gcgaccaaag agttcggtct
     2041 ggtttcttcc gtctcggtca tctttacact ggtgccatat ctttacacct gtgcggcgtt
     2101 actgctgctc ggccacggtc actttggtaa agcacgcccg gcatatctgg ctgttactac
     2161 cattgccttc ctctactgca tttgggccgt ggtggggtcc ggggcgaaag aggttatgtg
     2221 gtcatttgtc accctgatgg tcatcaccgc catgtatgcg ctgaattaca accggctaca
     2281 taaaaacccg tatcccttag atgcaccaat aagcaaagat taattcccct taatccagca
     2341 aacataaaag ccaaccttaa gaacttaagg ttggcttaat tttgctttgc gagcatatgc
     2401 gcactttgtt cgatggaaac accgtgatgt tgaagcgcct actaaaaaga ccctctttga
     2461 atttactcgc ctggctattg ttggccgctt tttatatctc tatctgcctg aatattgcct
     2521 tttttaaaca ggtgttgcag gcgctgccgc tggattcgct gcataacgta ctggttttct
     2581 tgtcgatgcc ggttgtcgct ttcagcgtga ttaatattgt cctgacacta agctctttct
     2641 tatggcttaa tcgaccactg gcctgcctgt ttattctgat tggcgcggct gcacaatatt
     2701 tcataatgac ttacggcatc gtcatcgacc gctcgatgat tgccaatatt attgatacca
     2761 ctccagcaga aagttatgcg ctgatgacac cgcaaatgtt attaacgctg ggattcagcg
     2821 gcgtgcttgc tgagctgatt gcctgctggc taaaaatcaa acctgccacc tcgcgtctgc
     2881 gcagtgttct tttccgtgga gccaatattc tggtttctgt actgctgatt ttgctggtcg
     2941 ccgcactgtt ttataaagac tacgcctcgt tgttccgcaa taacaaagag ctggtgaaat
     3001 ccttaagccc ctctaacagc attgttgcca gctggtcatg gtactcccat cagcgactgg
     3061 caaatctgcc gctggtgcga attggtgaag acgcgcaccg caacccgtta atgcagaacg
     3121 aaaaacgtaa aaatttgacc atcctgattg tcggcgaaac ctcgcgggcg gagaacttct
     3181 ccctcaacgg ctacccgcgt gaaactaacc cgcggctggc gaaagataac gtggtctatt
     3241 tccctaatac cgcatcttgc ggcacggcaa cggcagtttc agtaccgtgc atgttctcgg
     3301 atatgccgcg tgagcactac aaagaagagc tggcacagca ccaggaaggc gtgctggata
     3361 tcattcagcg agcgggcatc aacgtgctgt ggaatgacaa cgatggcggc tgtaaaggtg
     3421 cctgcgaccg cgtgcctcac cagaacgtca ccgcgctgaa tctgcctgat cagtgcatca
     3481 acggcgaatg ctatgacgaa gtgctgttcc acgggctgga agagtacatc aataacctgc
     3541 agggtgatgg cgtgattgtc ttacacacca tcggcagcca cggtccgacc tattacaacc
     3601 gctatccgcc gcagttcagg aaatttaccc caacctgcga caccaatgag atccagacct
     3661 gtaccaaaga gcaactggtg aacacttacg acaacacgct ggtttacgtc gactatattg
     3721 ttgataaagc gattaatctg cttaaagaac atcaggataa atttaccacc agcctggttt
     3781 atctttctga ccacggtgaa tcgttaggtg aaaatggcat ctatctgcac ggtctgcctt
     3841 atgccatcgc cccggatagc caaaaacagg tgccaatgct gctgtggctg tcggaggatt
     3901 atcaaaagcg gtatcaggtt gaccagaact gcctgcaaaa acaggcgcaa acgcaacact
     3961 attcacaaga caatttattc tccacgctat tgggattaac tggcgttgag acgaagtatt
     4021 accaggccgc ggatgatatt ctgcaaactt gcaggagagt gagtaaatga aaattctgat
     4081 tgttgaagac gatacgctgt tattgcaggg actgattctg gcggcgcaaa ccgaaggcta
     4141 cgcgtgcgat ggcgtgacaa ccgcgcggat ggcggaacaa agccttgagg acggtcatta
     4201 cagcctggtg gtactggatt tagggttacc cgacgaagat ggactgcatt ttctcgcccg
     4261 tatccggcag aaaaaataca ccctgccggt actgatcctc accgctcgcg atacgctgac
     4321 cgacaaaatc gccgggctgg atgtcggtgc cgatgactat ctggtgaagc cttttgcgct
     4381 ggaagagtta cacgcccgta tccgcgccct gctacgacgc cataataatc agggcgaaag
     4441 tgagctgatt gttggcaatc tgacgctgaa tatgggtcgc cgtcaggtat ggatgggcgg
     4501 tgaagagttg attctgacgc ccaaagaata tgctctgctg tcacggttaa tgctcaaagc
     4561 aggcagtccg gtacatcggg aaattctcta caacgacatc tataactggg acaatgaacc
     4621 ctcgaccaac accctggaag tgcatatcca caatctgcgc gacaaagtgg gcaaagcccg
     4681 tatccgcacc gtgcgcggct ttggctatat gctggtcgcg aatgaggaaa actaattgaa
     4741 tctgatgcgt tttctgcgcc gacctatatc gctgcgccaa cggctgatat tgaccatcgg
     4801 ggccattttg ttggtgtttg agctgatcag cgtcttctgg ctatggcatg aaagtaccga
     4861 gcagattcag ctgtttgagc aggcgctacg cgacaatcgc aacaacgatc gccacatcat
     4921 gcgtgagatc cgcgaggcgg tcgccagcct gattgtcccc ggcgtcttta tggtcagcct
     4981 gacgctattt atctgctatc aggcggtacg ccgcatcacc cgcccgctgg cggagctgca
     5041 aaaagagctg gaagcgcgca ccgccgacaa cttaacgccc attgccattc acagcgccac
     5101 cctcgaaatc gaagcggtgg tttcggcgtt gaacgatctg gtcagtcgcc tgaccagcac
     5161 gctggataac gaaaggttgt ttaccgctga cgtcgcgcac gaactgcgaa cgccactggc
     5221 gggggtgcgt ttgcatctgg aactgctggc gaaaacgcat cacattgatg tagcaccgtt
     5281 agtggcacgg cttgatcaga tgatggagag cgtctcccag ctgctgcaac tggcgcgtgc
     5341 cggacagtca ttttcttccg gtaattatca acatgtaaaa ctgctggaag atgtcattct
     5401 cccctcgtat gacgaactca gtaccatgct cgaccagcga cagcaaaccc tgctattgcc
     5461 agagagcgcc gccgacatca ccgttcaggg tgatgcaacc ctgctgcgga tgttattgcg
     5521 aaacctggta gaaaacgccc atcgttacag cccgcaaggc agcaacatta tgattaagct
     5581 gcaagaagat ggcggagcgg tcatggcagt tgaagatgaa ggaccaggta ttgatgagag
     5641 taaatgcggg gagttgagta aagcgtttgt acgtatggac agccgttatg gcgggattgg
     5701 tctggggtta agtattgtca gccgcattac acagttgcat cacgggcagt ttttcctgca
     5761 aaaccggcaa gagacttccg gcacgcgggc ctgggtacgg ctgaagaaag atcagaacgt
     5821 ggcaaaccag atataaagaa agctgctgac caccagcacg ctgaacacgg tagttaaact
     5881 ttcataaaca cggtttttca ttgcactctc ctccgttaac ctggaggaga gtatgcgcgt
     5941 cagagattaa gtgaacctta agagttcaat tggcctgata agacagcgtc acatcaggcc
     6001 atccgtttca gcttattcat caattcgcgg atgttgctgc accaggcggg tacgtttcgc
     6061 ctgcaaatcg gcaatctcgt ggtcaatatt atcgattttc tgctcgatat tatcgtaatg
     6121 ctcgacgaga atttccttcg cttcctgtat atctgacgcc gccggtgtcg cacctttcaa
     6181 cggacgattt gccgtctctt tcatggttac gccggtgatt aaaccaatca ccgccactac
     6241 catcaggtaa taggcaggca tcatcagatt ctgcgagctt tcgaccagcc aggccgccag
     6301 tgttggcgtc agaccggcaa ccagcaccga aatattaaat gccgccgcca gcgcgctgta
     6361 acggatatgc gtcgggaaca tcgctggcaa ggtagaagcc ataacgccca taaagcagtt
     6421 aaggatcacc gccagcatca gtaacccggc aaaaatcagg ccgatgacgt tactgttaat
     6481 cagaataaac gccgggatcg ccaacacaaa cagtgcaaca ctaccaagta gcacaaacgg
     6541 acgacggcca aaacggtcac tcagcaagcc catcaccggc tggacaaaca gcataccgat
     6601 cataatggcg ataataatca gcaccccgtg gtcttcggag taatgcaggt tatgcgacaa
     6661 ataactcggc atataggtca gcaacatgta gtaagtcacg ttggtggcaa ttaccagacc
     6721 aatacatgtc aacaggctgc gccagtattt agtggcaatc tctttaaacg agactttcgg
     6781 gccatcctgc aaaccttcgc ggtcgccctg ttccagttta tcaacatgct gctggaacgc
     6841 cggagtttct tccagcgcat ggcgcaggta aagcccgata atccctaacg gcagagcaat
     6901 aaagaacgga atacgccagc cccagtcgag gaagttcgct tcgccgacaa tggtcgaaat
     6961 taacaccacc acgcccgcac ccagcacaaa cccggcaata gaaccgaagt ccagccagct
     7021 gcccataaag ccacgtttac ggtcagggga gtattccgca acaaatatcg acgccccggt
     7081 atattcacca ccgaccgaga aaccttgtgc catcttacag atcaacagca gaatcggtgc
     7141 ccaaatacca atcgtgtcgt aggacggtat taagccaata cagaacgtac tgatcgacat
     7201 aatcacaata gtgatagcga ggatcttctg gcgaccatat ttatcgccca acataccaaa
     7261 gaagagtccg ccaagcggtc gaatcagaaa gggaacggag aaagtggcaa gtgcagcaac
     7321 catctgcacg ctggggtcag cccccgggaa aaaaacttta cctaatgcgt aagcaacaaa
     7381 accataaaca ccaaaatcaa accattccat tgcattaccc agtgatgctg cggtaatggc
     7441 tttacgcagt ttaccgtcat caataatggt gacatcacga agggtaatcg gttttacttt
     7501 tttccttttc agcatagctt tcctcgcaga gttggcccat ttccgcaaac gccagaaaaa
     7561 tcagcgcgac ggccgcaaaa ggggagcggg aattgcagga tgcaatctct gtaacaagcg
     7621 tagcgggttt gcttacaccc tccggttaag gaatgtacaa tcaaaccagt tgacgcctga
     7681 attgggcagt taatgacctt tttaccctac cagtgtttaa atttgtgatc aacttcacac
     7741 atggatttcg tttaggactc attgatgtaa ctgattattt acgccaaatt tacagagaag
     7801 atttcctcga ccattgcccc caccaaacgc tgcgctgaac agagtcgtgg catccccagc
     7861 gcgacagttt gccagcgttg ggtcactgac cagcaaacgg gttgtttatc tgccgtggtc
     7921 cactttccca gccaacttaa aaaatcagca tgatccccgc ccgtcggaac ggtcgaagag
     7981 gcaaaccact gatggtaata gtgacgcgta gtcggtgccg cattgatact ctctgccaga
     8041 tgttgtgtcg ctttctcgcg caaatcaccc agcaacaaca ccaccgcttg cgggcttccc
     8101 agacggcagg catcgccaaa tgcaccccag cgcaacccct ccagaacgat aaaacagcgc
     8161 ccaggcaacg accaggcgtt atacgcccca gcacgccagc gggtaaaaat ttgctcgctc
     8221 tgatcacctg cctgcaccag taggcctcgt tgcgttaacg ccttttcctt ccagcgacaa
     8281 cgggctgaaa accactcata caaggcggca atttgctggg ggagctccgg gaaagcagag
     8341 tgctggcggt gatccatttt ttgcagcagc gccagatgct gcgcgatctg cgtcagcgcc
     8401 agatgaccgg gatccagcat cgacgccagt ttttccacca ttgtcagtcg caggctaagc
     8461 ggcgcattta acatgccgcc gttcgtccat gtctgaagct gcacacgcga aagaagttcc
     8521 tgcatcagtc gttcacgaaa ctgctgctga tgggcttgag gatggcgggc atcatcgccc
     8581 tgcgccagat ccactaaaaa gcgcggatga accgactcca gcacgcgacc ggggccgtcc
     8641 agtaacgtct tggtcaatat cgttctgccg tgaaaagtgt ttgaatatca tcgcgtaaca
     8701 gctgagcgtc ggtgtaaatc cagctgtgag tcatcacagt ctgctgcaat tgctgctgca
     8761 tcagcctgac caccgattca ttttgttgac gcagagccag actttcgcgt aaacgcgtct
     8821 gtaattctgt caaacatgaa gcgaactcag cgaaaaaagt attcatgcct gccgtaacag
     8881 attcatcgac ctgctctgcc agaactttag ccatttgttg gcaatagaga tcgacttctg
     8941 cgcttaatgc tcgttgcaac acactgtaat caaccgtttc tgtcggggat ttctcatttc
     9001 cccgtcccca gtcgggctga ttcaaccagc gcgaaaaagt ctcacgcaca acgcctaaac
     9061 gcgtgctctg ctcgtccgtt gcttcctggc gcgaaatgac ttcactgaac agctggcgag
     9121 tgttgaagtg gggaactacg ccgtgaaaaa caggaaaatg aaacccagga cgaaaccctg
     9181 actcgctcaa ttccattttg acttgttgct caatggggcg aataacatcg gttaacaccc
     9241 ggcaaagggt ggattccatc tcggcaaaac gcagcgtaaa gtcgcgactg atggtgttct
     9301 gcgccgtctg taacagtgtc tcacagcggg tacgaatctc gcttaacggc tccgaatcat
     9361 cctgaaacaa ggcggctaac tgcgcattca ccgcatcttg ctgttgacgc agaaagtggt
     9421 tggcggaggt cagggccagc tcgatttcat gtttaatctc gccgctcacc tgcgcctgat
     9481 tgagttgcaa tagctgcaaa ctttcttcga cctgatggat attttgccgc aattgttcac
     9541 aagcgacgtt taacccgtgc gcacgaaaat ccaggtattc ccgcgcctgc tgcgcgtaat
     9601 tcaacagttt atgcgcagca gatcgcagag catacaatga ggcgttagcg taagcggcat
     9661 gaagcaacgc ctgaattggc tgggcgaaca gcgaatcttc ccacaactga tcggcagcat
     9721 gacgaatatg ttcgaggtcc gccagatcgg catgacgcca gcgcctgccg agcgcggcat
     9781 gggcaaaatc ttccacccag cgttgttgct ctggcgctgg taacttaccg ttgttggcta
     9841 actcatagcg cgcccgattc gccaggtagc cccacatcga cgacaccggg aatatctgct
     9901 gtggcgtaat acagcctttc atcagcgtcc cggaaatcag tgcccgcacc tggtcggcgt
     9961 cgtcactgtt acgatcctgt tgatcgaact tattgaccag cacatacagc ggcaccgatt
    10021 gccccaccgc caaaatcgcc tcacggacct cttcatcgga gatcgatttc agttgcgtat
    10081 aatccagcac cgccagtacc gccgaggcgc gtgccagctg ctggttaagc attttttgca
    10141 gatgcggttg cccggcttca tttggcccgg gggtatccag taacgtcaac tgaccgggat
    10201 aactttccag ccccgccaga tggacaaact ccacttcaat cacgggaata tgctcaatgg
    10261 cggcgtaagc agaaaaagga aaatcgacgt ccagcgcctt cgccagtcgc actaaatcat
    10321 tcaaactttt cagacaatga aaaataggct gggcacccag ataatatttt tcgaaagcga
    10381 cgccattttc gatccgctgc ataagcgcac gcatatcttt atctatttcc agcacatcgg
    10441 tcagatgctt aatatcgcaa tcacgcaggc gctgttgtaa ttgttgaatt aaacaatcga
    10501 ttggcgcgac atgtgaaaaa tgcagtaccg gttccttttg cccgggcgta tggcgaataa
    10561 gcgtcggcag cgcagtcatt gggcgattac gattaggcag aacctccgta ccaacaatgg
    10621 cattaatggt ggttgatttc cctgctttca tggtaccgac aattgccagc accatttcca
    10681 gtcgggaaat tttacacaac tcattattca gcatcgcgtg acgttcggcg atattaggct
    10741 gactccaggg taaagccagt tgtggcgcgt cgtctccggg tacagagaga ggcatttttt
    10801 ccagtaactg caactgttgg cgagaaagct gtaacaggcg ttcagcctcc tgacttaact
    10861 catacagggt ctgtgtgtac atagaaaatt cttccttaaa gcaaattttg ttacttttat
    10921 ttagccagat tgtttttgag ttctgttttc ggcttttata attactgcaa
//
LOCUS       DAZXUK010000129        10755 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZXUK010000129 DAZXUK010000000
VERSION     DAZXUK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10755
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.129"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            758..841
                     /gene="dinQ"
                     /locus_tag="ACH3L8_002582"
     CDS             758..841
                     /gene="dinQ"
                     /locus_tag="ACH3L8_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFT4085644.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACH3L8_002583"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACH3L8_002583"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFT4085645.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACH3L8_002584"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACH3L8_002584"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFT4085646.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="ACH3L8_002585"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="ACH3L8_002585"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFT4085647.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACH3L8_002586"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACH3L8_002586"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFT4085648.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="ACH3L8_002587"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="ACH3L8_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            7850..7903
                     /locus_tag="ACH3L8_002588"
     CDS             7850..7903
                     /locus_tag="ACH3L8_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085649.1"
                     /translation="MVLNFVLAGDFDCVRRS"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="ACH3L8_002589"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="ACH3L8_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFT4085650.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="ACH3L8_002590"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="ACH3L8_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFT4085651.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="ACH3L8_002591"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="ACH3L8_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFT4085652.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
BASE COUNT         2653 a         2763 c         2876 g         2463 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttga
//
LOCUS       DAZXUK010000130        10663 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZXUK010000130 DAZXUK010000000
VERSION     DAZXUK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10663
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.130"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1802)
                     /gene="selB"
                     /locus_tag="ACH3L8_002592"
     CDS             complement(<1..1802)
                     /gene="selB"
                     /locus_tag="ACH3L8_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFT4085653.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRG"
     gene            complement(1799..3190)
                     /gene="selA"
                     /locus_tag="ACH3L8_002593"
     CDS             complement(1799..3190)
                     /gene="selA"
                     /locus_tag="ACH3L8_002593"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFT4085654.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3288..3896)
                     /locus_tag="ACH3L8_002594"
     CDS             complement(3288..3896)
                     /locus_tag="ACH3L8_002594"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFT4085655.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3967..5052)
                     /locus_tag="ACH3L8_002595"
     CDS             complement(3967..5052)
                     /locus_tag="ACH3L8_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFT4085656.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5055..5417)
                     /locus_tag="ACH3L8_002596"
     CDS             complement(5055..5417)
                     /locus_tag="ACH3L8_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFT4085657.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5954..7867
                     /gene="mtlA"
                     /locus_tag="ACH3L8_002597"
     CDS             5954..7867
                     /gene="mtlA"
                     /locus_tag="ACH3L8_002597"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFT4085658.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            7962..9110
                     /gene="mtlD"
                     /locus_tag="ACH3L8_002598"
     CDS             7962..9110
                     /gene="mtlD"
                     /locus_tag="ACH3L8_002598"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFT4085659.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9110..9697
                     /gene="mtlR"
                     /locus_tag="ACH3L8_002599"
     CDS             9110..9697
                     /gene="mtlR"
                     /locus_tag="ACH3L8_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFT4085660.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9709..9918)
                     /gene="yibT"
                     /locus_tag="ACH3L8_002600"
     CDS             complement(9709..9918)
                     /gene="yibT"
                     /locus_tag="ACH3L8_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFT4085661.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10202..10564
                     /locus_tag="ACH3L8_002601"
     CDS             10202..10564
                     /locus_tag="ACH3L8_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFT4085662.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         2560 a         2882 c         2751 g         2470 t
ORIGIN      
        1 tttccacgac gacgcgtaaa gccaatgcgg tcaaaatatt ccagaatctg aattgccagc
       61 tttcggccta cgcctaagcg atcgcggaaa tccgccgcgc aggttgaacc acactcctga
      121 tcgagatcgc ggatcatatt ggcaaattcg acaatccgat cgttacggta ataacgatct
      181 ttgacgatcg cggtaattat tccttgctgc gccgcctggc gtagcgtcag gcgcattgcc
      241 tgctcgtcgg tacccgtctc ttttgccaga tcacgcaccc accacggctc gtcaccaaac
      301 agcggctctg ctttttgcca gatggcctgc tgctcttcgc tgaagcccgc tttgtgatcc
      361 ggcagatgca gccaaccgtg atgactgagg atgtcgccgc tttcgcgcat cttttcaatc
      421 agcaacagta ctagcgcttc atcttccatt ggcaacgcca tacgtcgcag acgttcgcgc
      481 ccaggcccag gttcatcgcg atgttgctca tgataagtcg ctaatgtgtc gagaattttc
      541 cgttgccagc gagcggcaac cggcgcattc aacaagctat aaccagcctg aatatagcca
      601 ggctgttgca gcagttcgcg catcccttcg ccgttgagct ggcgcgccca agcgaaatca
      661 ggaagattaa ccgcgccgcg ttgcaggtga acggctaacg cttcgctatc attctgtgcc
      721 ctggcgaggg aagccagcca ttgcagatac tctggtttac gtttaccgcg acgcggcggg
      781 ttaagcatca cgacgcgcgc tccggccagc gtgttgcggg cagagatatc gcgcaatacc
      841 aggcggtcgt tatctgccag ccataacggg gtgtcgaaga ccagttcagc aaggttatct
      901 tccagcagtg aaacgcgtcc cgtgacgtgg ctggcggcgt ggtgaatatg cagcggctgc
      961 cactgggtca gcggtgcatg ggtttgaagc tcgacaatca cccgtgtgaa cggctctggc
     1021 ggcacatcgg caagcagcca gtcgccacgg ttaatctgct ctttttccgc atcacccgcg
     1081 atgtcaagcg cgatacgctg cccggcattg gcggtttctg ttggctggtt ttgcgcatgc
     1141 agcgcacgta cacgcatcgg tttatttaca ccagtcagcc agagtgaatc gcctaccttc
     1201 acttccccgc ttaacgccgt accggtgacg accagcccgg caccttttac ggtaaatgcg
     1261 cggtcaatcg cgaggcggaa actatgttgg ctggcgtgct cgcgttccgg caactgaagc
     1321 agatgctcgc gcagggcatc aattccccga ccttcggttg ctgcggtgat aaacagtttt
     1381 gcctcagcaa aaccgtattc ccgcagaacc tcctttacct ggcgttcaac ctcatcaaca
     1441 cgcgcttcgt ccacgcgatc ggctttggtc agcgccactg tcagcatcgg gttaccggtc
     1501 agctgcaaaa tcgccagatg ctcacgggtc tgtgccatca cgccgtcatc gcacgccacc
     1561 accaacagcg cgtgatcgat accaccaacg cccgccagca tgttggaaag aaacttttca
     1621 tgaccgggaa cgtcgataaa accaggcacg cgaccatccg gctgcggcca gtaggcatag
     1681 ccgagatcga tggtcatgcc gcgctttttt tcttccggca gacggtcagc atttacgcca
     1741 gtaatcgcct gcaataaggt tgttttgccg tggtcaacgt gtccggcagt cgcaataatc
     1801 atttcaacaa catctccaaa aaccgttgct catcttcaag gcagcgtaaa tccagccaca
     1861 atcgtccgtc ataaatacga ccaatcaccg gcactggcaa ttcacgccag cgggcggcta
     1921 atgactcaag gtggctaccg cgtccatcat ggggtgtaaa cgttaatgcc gcgctcggca
     1981 ggcgatcaac cggcagcgaa ccactgccaa tctgcgaaag acatggcata acctgtaccg
     2041 caaactccgc gccgtaatgt gcgacaaggg gagcctgtaa acgttgtgcc tggatttgaa
     2101 tgacctctgc gctgcgggta agcaggcgca gggtcggtaa tttttcactc agagcttcag
     2161 ggtgtaaata aagacgcaac gtggcttcca gcgccgcgag ggtcatttta tccgcgcgta
     2221 atgcacgctt cagcgggtgg ctttgcaggc gggcgatcat ctctttttta ccaacgataa
     2281 tcccggcctg cggcccgcct aacaacttgt cgccggagaa gctcaccaga ctgacgcccg
     2341 ccgcaatcaa ctcctgcggc attggctctt tcggcaaacc gtactggcta agatcgacca
     2401 gcgagccact gcctaaatca gtcaccactg gaacatccag ctctttgccg agcgccacca
     2461 gttcagcttc gtctatcgct ttggtgaacc cctgaatgct gtagttactg gtatgtactt
     2521 tcatcaacag tgcggtattt tcattcaccg cctgacgata atcattcgcg tgcgtgcggt
     2581 tggtggtccc tacttcgtgt agggtgcagc ctgcctgacg cataacatcg ggaatacgaa
     2641 acgcgccgcc aatctccacc agttcgccgc gagataccac cacctctttc ccgctggcag
     2701 tggccgccaa catcaataac accgccgccg cattgttatt gacgatacag gcatcttccg
     2761 cccccgtaat acggcacagc agctgcgcca gcgcccgatc gcgatgtccg cgtccggcgt
     2821 cgtccagatc gtactcgagg gtcactggcg aacgcatagc ctgcgcaacg gcttccaccg
     2881 cggcttccgc ctgtaaagct cgcccaaggt tggtatgcag cacggttccc gtcaggttga
     2941 tcaccggacg cagcgcgctc tgcgcctctt tcgtcaaccg ggcatcgact tcttgcgccc
     3001 agttttcaca ccacgcaggc agcgtctggc tgccacgaat cacttctcgc gcttcgtcga
     3061 gcatctgacg caacaattcc accacgcggg tgtgaccata agtatcacgc aaagaaagga
     3121 aggagctatc gcgcaataag cgatcaatag ccggaagttg actatagagg gaacgcgttt
     3181 cggttgtcat aggaaacctg gctgatcaag gccctctcac acggagaagg gcgtttaaca
     3241 taaccacgga ttgtaacgtg agatgggtca ggaggacata tcgcgcatca agcctttggc
     3301 ggttcggtgc gagcaaaact ttcgcggcta aacaggtttt cgaccagttt gactaagtgt
     3361 gggcggtcga cacaccaacc tggcgcaacg cggcggaaat tgagatatcc gacagcacag
     3421 gcaatggcga tagtcgccag attgaccgta tcggttttga gtgtgccatc gaccagatat
     3481 ccttccagca catccagact gcggttgatt ttctcccgct ggcgtaacaa ttcatcttca
     3541 gactgctgcg ccgccggacg cgcctgttca cgcaccgata ccagcccggc atccataatg
     3601 ccatccgcca gcgcctcaat tttgcgcacc cgcaacgact ccagcggatc gcgcggcaac
     3661 atcgccggag cgacattcat taactcaata tattcagcga tgatcggcga atcaaaccag
     3721 cattcgccct cttcggtcac cagcaccggc actttcccta acgggttaaa ttgcgccacg
     3781 ccgttatccg cgttataggg cagttcatta ataaattcga aagttatgcc cttttctaac
     3841 aacagaataa aaagtttgcg tacaaacggg ctggtgtagc taccgacgag tttcatgccg
     3901 agtcctttgt gcgaggaaaa atatcagtat ggctcaggct cacgcaaaac acaggtgata
     3961 taagtctcaa tgatccaaat aaagataatg catccaactg gtcattctta gcagcacttt
     4021 ccgcatcacc gaaacatggc tgaaatggtc ggagtaaacc gccacctggg cgtagatgcc
     4081 gtcgggtaag gcatcgatat catcgttagg gtccagttca atggttccca gcacaccgtc
     4141 cgtgccggga acgaccgtta atgattgcaa taccccctgc gcctgataag aaccgcctgg
     4201 cacgacaggt aaaatactag tcaatttgcc gtggaacacc tgcccaggta gcgcgttaaa
     4261 caccacttcc gcatcatcgc caggtttcag acgtaacagc gagttttgcc gaaattgggc
     4321 gacaatttgc cgtttttgct cggggatgaa gaccatcacc ggacgcagcg gcaaggcagc
     4381 tgcgtatgta cctgggcgga tcagtacctg agtgacgtag ccattgcttg gtgcgcgaat
     4441 gacagtctgc tcaagattat attttgcttc agtaagttgc gctcttaagc tcacaatctg
     4501 cgattgctcg ccgttgacca tactgtcgag ctgactctgg atctgcgcct gctccgccac
     4561 cgagcctttc actaacgcat cctgcgcgag gaaattttgc cgcgcatcgt cgatgtcacg
     4621 ttccgagaac ggattcaccg ccgcctggct gcctttcagg taacgttgat aatttttaaa
     4681 cagacggtcg cgctccgctg aaacctgggt ggtgttggcc tgcgcttcag tgagctgcgc
     4741 acgcagcgtc tttatattat gcgtcgccgt catcaggtca gcctgaagtc tgtcaactcg
     4801 cgcctggtaa cgaaccgggt cgagcttaaa aagcacctcg cccttttgaa taagctgatt
     4861 attcttgtca gtgacttcag taacaattcc cgtcacctgt ggcgtgatag ggatcgctat
     4921 cactgccttt tgcgcggtaa aagtgtaagg gtggttgtag ttcatcaaca aaatcaaacc
     4981 actcaccaga aacacgcctc ccagcgccgc cgtcgccagc gccacgtaag ttaaaacaat
     5041 caatagatcc ataattagat ctccgggaac gtggaatagt cagtcttttt ctcggcggcg
     5101 agttgatgct ccagccggga aattcggtca gaaagtgcgg cgatttccgg ttcagtcgct
     5161 ttctcctgcg acgcaacatg tgactgcata ccccagccac gctccggttg atagagcgtc
     5221 gcccagatcc acagaaacgg ccagataaca tgcagagtaa acaggctcac ccagcccgcc
     5281 gtatgaatag cgtcggcatg ggggtgattg cgctttttgg caatcagata agggatgtca
     5341 tgtatcgcga tgattccata aaaaatcacc agaaagacga agatcagcac tcccaacgcg
     5401 aaatagttta gaaacatatc tgcctcggat tcacgtttat cagtgttgtt ttttggctgg
     5461 cagccagaag ggagtcaggc tgatattttg acaataatcc gggttcgcga ttctcgccat
     5521 aacaccaaag aataattttt agaggtgatg agttgcttag ttacataacg attgtatgac
     5581 gaaggcataa catgctgtag atcacatcag gtgaacgccg taagaaaata tcttgtgatt
     5641 cagatcacaa agattcaaca aaccatcaaa acaaaaatgt gacattactc acatttaaat
     5701 gccattttta gcgaaaatca ccgccttgtt tcttttttat acaggcgttt tgtgatgagc
     5761 gtcacgtcaa ttacctctct accccctata tttatgtgac tgatatcaca caaaagcccg
     5821 tcgactggac agttaaccga ttcagtgcca gatttcgcag tatctacaag gtccggctac
     5881 ctctgccgcc acattaacaa aaaacctcgg gcttccagcc tgcgcgacag caaacataag
     5941 aaggggtgtt tttatgtcat ccgatattaa gatcaaagtg caaagctttg gtcgtttcct
     6001 cagcaacatg gtgatgccaa atatcggcgc gtttatcgcg tggggtatca tcaccgcgtt
     6061 atttattcca acagggtggt taccgaacga gacgctggcg aagctggtcg ggccgatgat
     6121 cacttatctc ctaccgctgc tgatcggtta taccggtggt aagctggtag gcggcaaacg
     6181 tggtggcgta gtcggtgcca ttaccaccat gggcgttatc gtcggcgcag acatgccgat
     6241 gttcctcggt tctatgattg caggtccgct gggcggctgg tgcattaagc acttcgaccg
     6301 ctgggtagac ggtaagatca aatccggttt tgagatgctg gtgaataact tctccgcagg
     6361 catcatcggg atgatcctcg ctattctggc attcctcggc attggcccga ttgttgaagc
     6421 cctgtccaaa atgctggctg cgggcgttaa cttcatggtt gtccatgaca tgctgccgct
     6481 ggcgtctatc tttgttgaac cggcgaaaat cctgttcctc aacaacgcca ttaaccacgg
     6541 tatcttctcg ccgctgggta ttcagcagtc ccatgaactg ggtaaatcaa tcttcttcct
     6601 gattgaagct aacccaggtc caggtatggg cgtgctgctg gcgtacatgt tctttggtcg
     6661 tggtagcgct aaacagtctg cgggcggtgc ggcaatcatc cacttcctgg gtggtatcca
     6721 cgaaatctac ttcccgtatg tgctgatgaa tccgcgtctg atcctcgcag tcatcctcgg
     6781 cggtatgact ggcgtgttca cgctgactat cctgggcggt ggtctggttt ctccggcatc
     6841 tccgggttct atccttgctg tactggcgat gacaccaaaa ggtgcttatt tcgctaacat
     6901 cgcgggtgtg tgtgcggcga tggctgtctc cttcgttgtc tctgctattt tgctgaaaac
     6961 cagcaaagtg aaagaagaag atgatattga agcagcaact cgtcgtatgc aggacatgaa
     7021 agctgagtct aaaggcacat ctccgctatc tgcgggcgat gtgactaacg acctgagcca
     7081 cgtacgtaaa atcatcgttg cctgtgacgc cggtatgggt tcaagtgcga tgggcgcagg
     7141 cgtgctgcgt aagaaaattc aggatgcagg tctgtcgcag atttcggtca ctaacagcgc
     7201 gatcaacaac ctgccgccag atgtggacct cgtcatcact caccgtgacc tgaccgaacg
     7261 cgctatgcgc caggttccgc aggcacagca tatttcgctg accaacttcc tcgacagcgg
     7321 cctgtacacc agcctaaccg aacgtctcgt tgctgcccag cgccatactg aaaacgaagt
     7381 gaaagtgaaa gacagtctga aagacagctt tgacgattcc agtgctaacc tgttcaaact
     7441 gggcgcggag aacatcttcc tcggtcgcaa agcagcaacc aaagaagaag cgattcgttt
     7501 tgctggcgag cagctggtga aaggcggtta cgttgagccg gaatacgttc aggcgatgct
     7561 ggatcgtgaa aaactgaccc cgacttatct gggtgagtct atcgcggtgc cacacggtac
     7621 ggttgaagcg aaagatcgcg tactgaaaac gggcgtggtg ttctgccagt acccggaagg
     7681 cgtgcgcttc ggtgaagaag aagatgacat tgcccgtctg gtgattggta ttgcagcccg
     7741 taacaacgag cacattcagg ttatcaccag cctgaccaat gcactggatg atgagtccgt
     7801 catcgagcgt ctggcacaca ccaccagcgt ggatgaagtg ctggaactgc tggcaggtcg
     7861 taagtaatcc aatcctaccc tctcctcacg gagagggttt gggtgaggga aaagcctcac
     7921 cccagccctc tcgggtaaaa acattgatga aggttaatac tatgaaagca ttacattttg
     7981 gcgcaggtaa tatcggtcgt ggctttatcg gtaaactgct ggcagacgcg ggtatccaac
     8041 tgacgtttgc cgatgtcaat caggtggtac ttgatgccct gaatgcccgt catagctatc
     8101 aggttcatgt ggtcggtgaa accgagcagg tagataccgt ttccggcgtc aatgctgtca
     8161 gcagcattgg tgatgatgtc gttgatctga ttgctcaggt cgatttagtc actaccgccg
     8221 ttggcccggt tgtgctggaa cgtattgctc cggctatcgc caaagggctg gtgaaacgca
     8281 aagaacaagg taatgaatcc ccgctgaaca tcatcgcctg tgaaaacatg gtacgcggca
     8341 ccacgcagct gaaaggtcat gtgatgaacg ccctgccgga agacgccaaa gcgtgggtag
     8401 aagagcacgt tggctttgtc gattccgccg ttgaccgcat cgtaccgcct tcggcttcgg
     8461 caactaacga tccgctggaa gtgacggtag aaactttcag cgaatggatt gtcgataaaa
     8521 cgcagttcaa aggcgcactg ccgaacatcc caggcatgga gttaaccgac aacctgatgg
     8581 catttgtcga acgtaaactc ttcaccctga acacgggtca tgctataacc gcgtacctcg
     8641 gaaaactggc cggtcatcag accattcgtg acgctattct cgacgagaaa atccgcgcgg
     8701 tggtaaaagg tgcgatggaa gaaagtggtg cggtactgat caagcgctac ggctttgacg
     8761 cagacaaaca tgcggcgtac atccagaaaa tcctcggtcg ttttgagaac ccgtatctga
     8821 aagatgatgt agagcgcgta ggccgtcagc cgctgcgtaa actgagtgct ggcgaccgtc
     8881 tgatcaagcc actgctcggt acgctggaat acagccttcc gcacaagaat ctgattcagg
     8941 ggattgctgg tgcaatgcac ttccgcagtg aagatgatcc gcaggctcag gaactggcag
     9001 cactgatcgc tgacaaaggt ccgcaggcgg cgctggcaca gatttccggt cttgatgcca
     9061 acagcgaggt tgtatccgag gcggtaaccg cttataaagc aatgcaataa tggtggacca
     9121 ggcgcaggac accctgcgcc cgaataacag attgtcagat atgcaggcaa caatggaaca
     9181 aacccaggcc gttgaaaacc gtgtgcttga gcgtctgaat gctggcaaaa ccgtgcgaag
     9241 ctttctgatc accgccgtcg agcttttgac cgaggcggta aatcttctgg tgcttcaggt
     9301 attccgcaaa gacgattacg cggtgaagta tgctgtagaa ccgttactcg acggcgacgg
     9361 tccgctgggc gatctttctg tgcgtttaaa actcatttac gggttgggcg tcattaaccg
     9421 ccaggaatac gaagatgcgg aactgctgat ggcattgcgt gaagagctaa atcacgacgg
     9481 caacgagtac gcctttaccg acgacgaaat ccttggaccc tttggtgaac tgcactgcgt
     9541 ggcggcgtta ccaccgccgc cacagtttga accagcagac tccagtttgt atgcaatgca
     9601 aattcagcgc tatcaacagg ctgtgcgatc aacaatggtc ctttcactga ctgagctgat
     9661 ttccaaaatc agcttaaaaa aagcctttca aaagtaagca acgtctgctt actgcccctc
     9721 tacctgcttc ggccgataaa gccgacgata atactccaga cgttgtagat ataacggcac
     9781 gctttcatcg ggtattccgg acggaatcgc gttacgggga ggaagttttt tcagatactc
     9841 ccggaacgcc tggcttgatg ccatgaaatc tacggcttta tcgataagaa gcggaacgtt
     9901 ttcacctaat ttgcccatga tgatatctcc gtattacccc gcgccgggaa tgcgcggccg
     9961 ccaattttag tttaggttcc cggtaaagat gacattaagg aaaacgtgct gaatcctcaa
    10021 taatcatccg ctgctgttta ttatcttttt ctatagattt cttaattaat caacgaattg
    10081 cgtagttttt gagcatgtta atgattacgt tgcagaacta ttacaatgtc atcagttgtg
    10141 aacaaagcac ctggtcgcgc atactagggg ctataaattt atctttatca gaagccatca
    10201 catgaaagaa gtcgaaaaaa acgaaatcaa gcgtctcagc gatcgcctgg acgccatccg
    10261 ccaccagcag gccgatctgt cgctggttga agccgcagac aaatatgccg agctggaaaa
    10321 agagaaagcc acgctggaag cagaaattgc tcgcctgcgt gaggttcata gccaaaaact
    10381 gagtaaagaa gcacagaagc tgatgaagat gccgttccag cgcgcaatta ccaaaaaaga
    10441 gcaggctgat atgggcaagc tgaagaaaag tgttcgcgga ctggtggttg tgcacccaat
    10501 gaccgcgctg gggcgcgaaa tgggcctgga agagatgact ggattttcta agactacgtt
    10561 ttaagcccct cagacgactg gttcgatcgt atcaacccgc atctggcttg ccttagctgg
    10621 tcaatgcggg gactctcctg tctactcgcc taattaccaa aca
//
LOCUS       DAZXUK010000131        10647 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZXUK010000131 DAZXUK010000000
VERSION     DAZXUK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10647
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.131"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="ACH3L8_002602"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="ACH3L8_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFT4085663.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="ACH3L8_002603"
     CDS             complement(1614..1937)
                     /locus_tag="ACH3L8_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFT4085664.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACH3L8_002604"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACH3L8_002604"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFT4085665.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACH3L8_002605"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACH3L8_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFT4085666.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACH3L8_002606"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACH3L8_002606"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFT4085667.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACH3L8_002607"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACH3L8_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFT4085668.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="ACH3L8_002608"
     CDS             7537..8574
                     /locus_tag="ACH3L8_002608"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFT4085669.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENSCQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACH3L8_002609"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACH3L8_002609"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFT4085670.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="ACH3L8_002610"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="ACH3L8_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFT4085671.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACH3L8_002611"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACH3L8_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFT4085672.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
BASE COUNT         2647 a         2710 c         2737 g         2553 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaactc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgtt
//
LOCUS       DAZXUK010000132        10503 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZXUK010000132 DAZXUK010000000
VERSION     DAZXUK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10503
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.132"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..510
                     /locus_tag="ACH3L8_002612"
     CDS             <1..510
                     /locus_tag="ACH3L8_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFT4085673.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACH3L8_002613"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACH3L8_002613"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFT4085674.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="ACH3L8_002614"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="ACH3L8_002614"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFT4085675.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="ACH3L8_002615"
     CDS             complement(2878..3075)
                     /locus_tag="ACH3L8_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFT4085676.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACH3L8_002616"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACH3L8_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFT4085677.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACH3L8_002617"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACH3L8_002617"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFT4085678.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACH3L8_002618"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACH3L8_002618"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFT4085679.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACH3L8_002619"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACH3L8_002619"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFT4085680.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACH3L8_002620"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACH3L8_002620"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFT4085681.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACH3L8_002621"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACH3L8_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
BASE COUNT         2478 a         3065 c         2704 g         2256 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcataggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc cgcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct ggcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggt
//
LOCUS       DAZXUK010000133        10465 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZXUK010000133 DAZXUK010000000
VERSION     DAZXUK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10465
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.133"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            3..203
                     /gene="cspG"
                     /locus_tag="ACH3L8_002622"
     CDS             3..203
                     /gene="cspG"
                     /locus_tag="ACH3L8_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFT4085682.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            214..402
                     /locus_tag="ACH3L8_002623"
     CDS             214..402
                     /locus_tag="ACH3L8_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFT4085683.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            377..607
                     /gene="ymcE"
                     /locus_tag="ACH3L8_002624"
     CDS             377..607
                     /gene="ymcE"
                     /locus_tag="ACH3L8_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFT4085684.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            597..770
                     /locus_tag="ACH3L8_002625"
     CDS             597..770
                     /locus_tag="ACH3L8_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFT4085685.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(819..1892)
                     /locus_tag="ACH3L8_002626"
     CDS             complement(819..1892)
                     /locus_tag="ACH3L8_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFT4085686.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1994..4705)
                     /gene="torS"
                     /locus_tag="ACH3L8_002627"
                     /pseudo
     CDS             complement(1994..4705)
                     /gene="torS"
                     /locus_tag="ACH3L8_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4788..5816
                     /gene="torT"
                     /locus_tag="ACH3L8_002628"
     CDS             4788..5816
                     /gene="torT"
                     /locus_tag="ACH3L8_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFT4085687.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5789..6481)
                     /gene="torR"
                     /locus_tag="ACH3L8_002629"
     CDS             complement(5789..6481)
                     /gene="torR"
                     /locus_tag="ACH3L8_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFT4085688.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6568..7740
                     /gene="torC"
                     /locus_tag="ACH3L8_002630"
     CDS             6568..7740
                     /gene="torC"
                     /locus_tag="ACH3L8_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFT4085689.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDITGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7740..10280
                     /gene="torA"
                     /locus_tag="ACH3L8_002631"
                     /pseudo
     CDS             7740..10280
                     /gene="torA"
                     /locus_tag="ACH3L8_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10277..>10465
                     /gene="torD"
                     /locus_tag="ACH3L8_002632"
     CDS             10277..>10465
                     /gene="torD"
                     /locus_tag="ACH3L8_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFT4085690.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2525 a         2684 c         2789 g         2467 t
ORIGIN      
        1 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
       61 atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca
      121 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
      181 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
      241 ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga gcgaaaccct
      301 ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa cgtcgcgctg
      361 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg
      421 agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa gcgataacct
      481 gttgattatt gaatctttcg ggaagatggc ttataacatt tcttacctga ccagggtacc
      541 gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg ttaacagtga
      601 atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa
      661 tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc atcgcacgag
      721 aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac
      781 atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
      841 tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac
      901 attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca
      961 gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt caatgttgag
     1021 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
     1081 attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc acaatcgata
     1141 caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc
     1201 gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc
     1261 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta
     1321 acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
     1381 aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg cagaggaata
     1441 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
     1501 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
     1561 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
     1621 cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
     1681 cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg cacccaatac
     1741 cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt cgctttacgc
     1801 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
     1861 cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
     1921 caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc gcgttcaggt
     1981 ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
     2041 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc
     2101 ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctgtcct
     2161 ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt ttaaataatg
     2221 ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg agttgcgaga
     2281 catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt aactgaccga
     2341 gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg gtacgctggc
     2401 gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct
     2461 gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg
     2521 cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaatag
     2581 caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
     2641 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg gctgtttttg
     2701 gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
     2761 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
     2821 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
     2881 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
     2941 catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg gcattgctca
     3001 ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg ctcggtacat
     3061 catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
     3121 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact gaaacatttt
     3181 tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt
     3241 cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc gttaagtgcg gggttatctg
     3301 ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
     3361 ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
     3421 gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
     3481 cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg
     3541 tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
     3601 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gaatgagcga taaaccacgc
     3661 gccagaggat cataatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
     3721 gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgtgc agctcaatgg
     3781 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata ttattttggg
     3841 cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc
     3901 tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa
     3961 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
     4021 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
     4081 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
     4141 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
     4201 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgctccagg cgtgcgatct
     4261 catcggcggc agctatctgc tgactgagtt gccgttgttg ctggcgtagt tgcagacgcc
     4321 gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc tgttgttcaa
     4381 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
     4441 tttatgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca
     4501 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
     4561 tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag cgcaggttgt
     4621 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
     4681 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc
     4741 aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
     4801 tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac ctgttgcgct
     4861 ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc aaacgcgtat
     4921 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
     4981 gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct
     5041 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
     5101 ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag gtagcaaatc
     5161 tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc cgcgttggtg
     5221 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
     5281 aaccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt aaggagatgg
     5341 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
     5401 gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa cgccacccag
     5461 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
     5521 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
     5581 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
     5641 cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
     5701 cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc tcgctgtcac
     5761 cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
     5821 caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga cgaattaaca
     5881 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgcagcagac
     5941 gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt tcatactctg
     6001 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
     6061 aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
     6121 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
     6181 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
     6241 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
     6301 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
     6361 tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt
     6421 aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac aataacaatg tgatgtggca
     6481 tcagagggtt ttactcattc tgttcatatc tgttcatatt tgctcattaa gatcgcttca
     6541 ctaaaccata attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc
     6601 agtgctcgtt ggtcggtact ggcgctggtc gcaattggga ttgtgattgg cattgcgctg
     6661 attgtattgc cacacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc
     6721 cacagtatgc aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc
     6781 ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg atattacagg catggtgaag
     6841 cgcaaactgg aagcgagcaa cgacatttac cagaccttta ttgcccactc cattgataca
     6901 cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa
     6961 gaaaacaact cggcaacctg tcgctcctgc cataactacg atgcgatgga tcatgccagg
     7021 cagcatcctg aagcggcacg tcagatgaag gtggcagcga aagataatca atcctgcatc
     7081 gactgtcata aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag
     7141 ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt
     7201 aagccaattt atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg
     7261 gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg
     7321 gaaagcgccg gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc
     7381 tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg agaaaaccac cgttgccgac
     7441 accaataccg agtggagcaa gttgcaggcc accgcgtgga tgaagaaagg cgacatggtg
     7501 aacgatatca aaccgatctg ggcctatgcg gattcgttgt acaacggcac ctgtaacgag
     7561 tgccacggcg caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac
     7621 ggcatgattg gctttaccag tctcgataaa cgtgaagaac gcactttgtt gaaatatctg
     7681 caaatgaatg cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa
     7741 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
     7801 taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc gcgccgtgca actgcggcgc
     7861 aagcggcgac tgaggctgtc atctcgaaag agggcattct taccgggtcg cactgggggg
     7921 ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata
     7981 aatatccgtc gaaaatgatc gccggattgc cggatcacgt acacaacgcg gcacgtattc
     8041 gttatccgat ggtacgcgtg gactggctgc gtaagcgcca tctgagcgac acctcccagc
     8101 gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag
     8161 aactggaacg cgtacagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc
     8221 aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg
     8281 gtaatagcgt tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc
     8341 gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga
     8401 acagcaaaac cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt
     8461 ggtgcccgga tcacgatgtt tatgaatatt acgagcagct aaaagcgaaa gtcgccgccg
     8521 gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc
     8581 gcgagcatgt gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc
     8641 tggcacatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct aactactgtg
     8701 tgggttttga gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg
     8761 ccgcatgggc tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc
     8821 agatggcggc gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg
     8881 gtgaacagtg ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc
     8941 caggtggtgg ttttggctgg cactacaacg gcgcaggcac gccggggcgt aaaggcgtta
     9001 ttctgagtgg tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca
     9061 aaggctacag cagcactatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga
     9121 aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg
     9181 ccggaactaa cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca
     9241 agctggaaac ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata
     9301 tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact
     9361 ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc gtagttcgag gcgcgcaacg
     9421 actttgatat tttccgcgag ctgtgccgtc gctttaatcg tgaagaagcc tttaccgaag
     9481 ggctggacga aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag
     9541 gacgcggcgt tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt
     9601 ttgaccatcc gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac
     9661 cgctgggcac gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact
     9721 acgacgattg tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc
     9781 ccggctcgca gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact
     9841 cgcagctatg tgagtcggaa acgctgcgtc agcaatatac ggtagcgggt aaagagccag
     9901 tgttcattaa cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg
     9961 tctttaacgc tcgcggtcag gtgttggcag gagcagtagt ttctgaccgc tatgcacccg
    10021 gcgtggcgcg aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg
    10081 cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga catcgggact tcgcagctgg
    10141 cgcaggcgac cagtgcgcac actacgctgg tggaaattga gaagtacaat ggaacagtgg
    10201 agcaggtgac ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg
    10261 cgtcgcaggt gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg
    10321 gctggcgcag ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc
    10381 gcagatggct gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa
    10441 tgatctggaa aacagtattg ccacg
//
LOCUS       DAZXUK010000134        10446 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZXUK010000134 DAZXUK010000000
VERSION     DAZXUK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.134"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..794
                     /locus_tag="ACH3L8_002633"
     CDS             <1..794
                     /locus_tag="ACH3L8_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shiE"
                     /protein_id="HFT4085691.1"
                     /translation="SETKAAYRCFSNDKVSANKIMTPHKENITERAQQFKRVLVLQDT
                     TELNYSGQKEKQGVGPKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKN
                     TRQEICNDLLHKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFER
                     AAQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQ
                     ARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="ACH3L8_002634"
     CDS             complement(744..1937)
                     /locus_tag="ACH3L8_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4085692.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="ACH3L8_002635"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="ACH3L8_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFT4085693.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="ACH3L8_002636"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="ACH3L8_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFT4085694.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="ACH3L8_002637"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="ACH3L8_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFT4085695.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="ACH3L8_002638"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="ACH3L8_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFT4085696.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="ACH3L8_002639"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="ACH3L8_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFT4085697.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2560 a         2656 c         2749 g         2481 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgaccccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcata
//
LOCUS       DAZXUK010000135        10370 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZXUK010000135 DAZXUK010000000
VERSION     DAZXUK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.135"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1407
                     /gene="norV"
                     /locus_tag="ACH3L8_002640"
     CDS             <1..1407
                     /gene="norV"
                     /locus_tag="ACH3L8_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFT4085698.1"
                     /translation="VGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEKNVLIDTVDHKF
                     SREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYCTANAIDSINGH
                     HHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDAVLFSNDAFGQH
                     YCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNLPVDMIATSHGV
                     VWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGIAETDPRVAVKI
                     FNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLRFRNKRASAFGS
                     HGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREIARQWALAPLPQ
                     STVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGTPWSEVPDNFLC
                     PECSLGKDVFDELASEAK"
     gene            1404..2537
                     /gene="norW"
                     /locus_tag="ACH3L8_002641"
     CDS             1404..2537
                     /gene="norW"
                     /locus_tag="ACH3L8_002641"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFT4085699.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(2665..4917)
                     /gene="hypF"
                     /locus_tag="ACH3L8_002642"
     CDS             complement(2665..4917)
                     /gene="hypF"
                     /locus_tag="ACH3L8_002642"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFT4085700.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(5070..5597)
                     /gene="hydN"
                     /locus_tag="ACH3L8_002643"
     CDS             complement(5070..5597)
                     /gene="hydN"
                     /locus_tag="ACH3L8_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFT4085701.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(5746..6756)
                     /gene="ascG"
                     /locus_tag="ACH3L8_002644"
     CDS             complement(5746..6756)
                     /gene="ascG"
                     /locus_tag="ACH3L8_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFT4085702.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(6818..6889)
                     /locus_tag="ACH3L8_002645"
                     /pseudo
     CDS             complement(6818..6889)
                     /locus_tag="ACH3L8_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6899..8608)
                     /gene="hycE"
                     /locus_tag="ACH3L8_002646"
     CDS             complement(6899..8608)
                     /gene="hycE"
                     /locus_tag="ACH3L8_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFT4085703.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(8626..9549)
                     /gene="hycD"
                     /locus_tag="ACH3L8_002647"
     CDS             complement(8626..9549)
                     /gene="hycD"
                     /locus_tag="ACH3L8_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFT4085704.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(9552..>10370)
                     /locus_tag="ACH3L8_002648"
     CDS             complement(9552..>10370)
                     /locus_tag="ACH3L8_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HFT4085705.1"
                     /translation="SVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMA
                     ALPPLNGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVT
                     FLGAPRTKEAENATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANT
                     TVSQPMITLLLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAM
                     PVKQAFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
BASE COUNT         2466 a         3003 c         2762 g         2139 t
ORIGIN      
        1 gtgggtcaac gtgactggga agtgcgtgat tttcacggca cggaatataa aacgctgcgc
       61 ggcagcagct acaacagcta cctcatccgc gaagaaaaaa acgtgctgat cgacaccgtc
      121 gaccataaat tcagccgcga atttgtgcag aacctgcgta atgaaatcga tctggcggat
      181 atcgattaca tcgtgattaa ccatgcagaa gaggaccacg ccggggcgct gaccgaactg
      241 atggcgcaaa ttcccgatac gccgatctac tgtactgcca acgctatcga ctcgataaat
      301 ggtcatcacc atcatccgga gtggaatttt aatgtggtga aaactggcga cacgctggat
      361 atcggcaacg gcaaacagct catttttgtc gaaacaccaa tgctgcactg gccggacagc
      421 atgatgactt acctgacagg cgacgcggtg ctgttcagta acgatgcttt cggtcagcac
      481 tactgcgacg agcatctgtt caacgatgaa gtggatcaga cggagctttt cgagcagtgc
      541 cagcgttact acgccaatat cctgacgccg ttcagccgcc tggtaacgcc gaaaattacc
      601 gagatcctgg gctttaactt accagtcgat atgatagcga cctcccacgg cgtggtctgg
      661 cgcgataacc cgacgcaaat tgtcgagctg tacctgaagt gggccgctga ttatcaggaa
      721 gacagaatca ccattttcta cgacaccatg tcgaataaca cccgcatgat ggctgacgct
      781 atcgcccagg gaattgcgga aaccgaccca cgcgtggcgg tgaaaatttt caacgtcgcc
      841 cgaagcgata aaaacgaaat cctgaccaat gtcttccgct caaaaggcgt gctggtcggc
      901 acttcgacga tgaataacgt gatgatgccg aaaatcgccg ggctggtgga ggagatgacc
      961 ggattacgct tccgtaacaa acgcgccagt gctttcggat ctcacggctg gagcggcggt
     1021 gcgatggatc gcctttccac gcgcctgcag gatgcgggtt tcgaaatgtc gctcagcctg
     1081 aaagccaaat ggcgaccaga ccaggacgct ctggagttat gccgtgaaca cggtcgcgaa
     1141 atcgcccgtc agtgggcgct cgcgccgctg ccgcagagca cggtgaatac ggtagttaaa
     1201 gaagaaacct ctgccacgac gacggctgac ctcggcccac gaatgcagtg cagcgtctgc
     1261 cagtggattt acgatccggc aaaaggcgag ccaatgcagg acgttgcgcc aggaacgccg
     1321 tggagtgaag tcccggataa cttcctctgc ccggaatgct ccctcggcaa agacgtcttt
     1381 gacgaactag catcggaggc aaaatgagca acggcattgt gatcatcggt tcgggcttcg
     1441 ccgcccgcca actggtgaaa aatattcgca aacaggacgc cactattcca ttaaccctga
     1501 ttgccgccga cagcatagat gagtacaaca aacccgacct cagccatgtt atcagtcagg
     1561 ggcaacgtgc cgacgacctt acccgccaga cggcgggtga atttgccggg cagtttaatc
     1621 tgcgcctgtt tccgcacacc tgggtgacgg atatcgatgc cgaagcccat gtggtgaaaa
     1681 gccagaataa tcagtggcaa tacgacaagt tagtgctggc aaccggtgcc agcgcctttg
     1741 tcccgcctgt gcctgggcgt gagttaatgc tgacgttaaa tagtcagcaa gagtatcgcg
     1801 cctgtgaaac gcaactgcgg gatgcccgac gcgtgttgat tgtcggcggt ggtttgattg
     1861 gtagcgaact ggcgatggat ttttgtcgtg caggcaaagc ggtcacgcta atcgacaacg
     1921 ctgccagtat tctggcgtca ttaatgccac cagaagtaag cagccgcttg cagcatcggt
     1981 tgacggagat gggcgttcat ctgctgttga aatctcagtt gcaggggctg gaaaaaacgg
     2041 attctggcat tctggcaacg ctggaccacc agcgtagtat cgaagtggat gcggtaattg
     2101 ccgccaccgg actgcgcccg gaaacagccc tggcacgacg cgccgggctg acgattaatc
     2161 gcggcgtttg cgtcgatagt tatctgcaaa ccagtaatgc cgatatttac gcgctgggcg
     2221 attgcgcgga aattaacggt caggtattgc cgttcctgca gccgattcaa cttagcggga
     2281 tggtgctggc aaaaaatctt ctcggcaata acacgccgct gaaactaccg gcgatgctgg
     2341 tgaaaatcaa aacgccggaa ttaccgctgc atctggcagg cgaaacccag cgtcaggatt
     2401 tacgctggca gattaatacc gaacgccagg gaatggtggc gcgcggcgtt gacgatgctg
     2461 accagcttcg cgcctttgtg gtcagtgagg atcggatgaa agaggcattt gtattgttga
     2521 aaacgttgcc ggtgtaggtg ggctactgtg cctaaaatgt cggatgcgac gctggcgcgt
     2581 cttatccgac ctacggggac gcatgtgtag gccggataag gcatttacgc cgcatccggc
     2641 aagggtgtcc aaatgcaaca cgctttatcc gttctggact tcacccgcta accaacgcgc
     2701 cgcagcaata accccctgcc ccagagacaa accgccatca cccgccggta aactctgtgg
     2761 aaagagcaat gtgaaatcag cgagataatg cgccagacgt gcacgcagca aacggttatg
     2821 aataaccccg ccgctaaata ccagcgtagt gataccacgc atcgtggcct gctcacgcat
     2881 caacgcggca aaaccctgcg ccagcgcatc atgaaacgcc cacgcgcgtt gattaaccag
     2941 tgcctgccag ttcagccact gctgccagaa agtggcgaga tccagttgat tgtccacccg
     3001 cggcatcgtc accggatgcg tcactccgtc gcatgaggct gcgagcgcct ccagagcaca
     3061 agccgcttca ccttcataac ttaacgtggc tggcgcacag cccagtgccg ccgccactgc
     3121 atcgaacaac cgcccacacg atgatgccag cggcgcgtta attccacgct caatggcccg
     3181 cgccagtacg ctccagtttt gctgttgcac acttgccgtt tcagggtaat tctgccactc
     3241 cggcacaaag cgcaggcact gcgccagcag gtttcgccac ggctgcttcg ccgccaaatc
     3301 gccacccgga agcgccactg caggcaagcc gcccaggtgc tcacattcgc gatagttcac
     3361 ccgcaggcac tcgccgcccc acaaagcgcc attctccccc ataccgatac cgtcgagcgt
     3421 caaagcaatg acatcaccgc catccagcgg ccactgatgc tctgccagac acgccgctgc
     3481 atgggcatga tgatgcagta ccgtttgcgt cggcagattc atttcgctcg cccactggct
     3541 ggagacatag cccggatgca catcatgcac aatgtattgc ggagtgaaat cgtagatgtt
     3601 ttgcatcagg cgtaacgctt cgcgccactg catctggatg ccatcgtcac ttaaatcgcc
     3661 cagatgctga ctcaacaccg cttgttcacc gcgcaccagg cagaaggtat ttttcagatc
     3721 cgcgccaaga cacagcacag gcggaacatt tttaaagccc ggaggcaaaa ccagcgcatc
     3781 cggcacatac ccccgcgaac ggcgcagcat ttcgccgctt tcgcgcacca ctgaatcatc
     3841 catccgctgc acgatgtcgc ggttatgtag caagaatccg tcggcaatgc cctgcaaatc
     3901 cgccagcgcc tgttcgttgc tgatagctgg tggtttaccg ctcaggttgc cggaggtcat
     3961 caccagcggg cattgcagtt cctgtaacag caaatgctgg agcgggttcg caggcaacat
     4021 caccccgact tcgttaaggc caggggcgat atcatcacaa agctcaggta cgtatttttt
     4081 atccaccagc acaatcggcg cagcgggcgt ggtaagcaac tggcgcgcag cgtctggtaa
     4141 actttctgcc actggcaaca tgactgccag cggtttcgcc gggcgatgtt tgcgctcccg
     4201 cagtgtcgcc accgcgttac tgttacgtgc atcgcaggca agatgaaatc cgccaatccc
     4261 tttgatggcg acaatgttgc ccatttttaa ctgtgcgata gctgcctgta atgccgcctc
     4321 ttgttctgca tgttcaccat gacttaccca ttcaagatgc gggccacact ccgggcaggc
     4381 caccggctgg gcgtggaagc gacgatcgag cgggtcgcgg tactctttgt cacaggccgg
     4441 acatagcgga aacgccgcca tcacggtaaa cgggcggtcg taaggcatcg cgcgaataat
     4501 ggtgaaacgc gggccgcagt gggtacagtt gataaacgga taacgataac gccgctcgcc
     4561 tggggtattc atttcggcaa ggcaagcagg gcaagtggcg gcatccggga caatttgcgt
     4621 attcatggtg ccgccggtgc tctggcggat ggtgaactcg gtgggcagtt gtgaccagat
     4681 aaacggctca cgctcgacgc tatcaatacg cgccagtggc ggacagtgct gatgcaattg
     4741 aacaagaaac gtttccgggt cttccagcag ccggacttct acgccatcgc cgtcattaca
     4801 tacatcgccg tgaagattta attgctgtgc cagctgccag acaaacggac gaaaaccgac
     4861 gccctgcact ttgccacgaa tacgcagttg gacaccgcaa gatgtgtttt ttgccattga
     4921 gttattcccg ccatcatgaa ttgcgtaacc cgccctgccg gacacgacag cgtcgtatcc
     4981 ggcagtctca ggtcggcgat accgcccgct ccgtattcta cgaatatttc cgggaattcc
     5041 tttgatgcca gaacagttct gtaagatttt tagaacatca gcgccgtacg gcggcgtttt
     5101 tctgcgctca gttgttcaag tttattacga tcgacacaaa tcagcgcatg ggtcgggcaa
     5161 gccgccatac acgccgggcc gtcttcacga tggttgcaca ggtcgcattt attggcttcg
     5221 gctttgtcag cccgtacatt cagacccgcg ccgctgttgc gaatcacagg acgtaccacc
     5281 acttccatcg cgccatacgg gcaagccacc acgcaggttt tgcaaccaat gcaacgttcc
     5341 tgcatcacag gaacaaaccc tttatcacgg ctgatagcac cattcgggca gacgttagcg
     5401 cacggtgcat cttcacactg acggcaaact gtcgccgtgg aaatgttcac acctttaatg
     5461 acatggatac gcggtaaaaa tgtttccggg gtcagcgatg cacagtcctg attttcctga
     5521 tgagaaacca cgcacgccac ttcacaggta cggcaaccaa tacatttact cgcgtcagca
     5581 atgatgaaac ggttcatcaa attctccagc aatgacagtt aatgcgccga tacactcaca
     5641 aatcatgcca gtttttaatt tgctgttatt taaggaaatt aatttctgta atgcaggaaa
     5701 aaagatgtca tcgacactag tgacgatgac atgtgatgac aatgtttatc gcgaaagggc
     5761 aatgagtgag tcgcggcgga tcagttttcc gctaaaggtt ttcggcggtg agaaatcccc
     5821 gccatcgagc ataaaaatca gccgtccaat aatttcctga atcatctcag tcaccggaat
     5881 ttttacgctg gagagcgccg gaacgatata gggggcaatt gcgatatcat cgaatccgat
     5941 aactgacacc tgctctggca ccgctacgcc gcgctcgtgc aacgctttga tcgcacctat
     6001 cgccatatcg tcgttactgg caactaacgc gctaaattta gcccctcgtt cgagcaacgt
     6061 ttctacccct tcggccccgc tggcgggcgt ccatttaccg ttagcgataa gtttttcatt
     6121 gagcgcaata ccatgctgcg acagcgcgtc tttatacccg gcaagacgtt caatgctggt
     6181 gggggaatcc atcgagccgg taaggaaagc aatctcctga tgcccggcgt ttatcaactc
     6241 tgccacggcg ttaaagctgg tctgtttatg atcgcaccag acgctatggc tgctattatt
     6301 gcgcaggcgg cgattaagca ccattatcgg ctgactgtgc gcgtcaatga tgtcatcaat
     6361 ctcatccacg cttaaaaaac gcgggtaaat cataatggcg tcgcagcgca aatccagcag
     6421 atactgaatc gcctggcgct cttcttcggc gctgtgttta ccatctgcca atagcaactg
     6481 tcgccctttc tcttccgcca ttcgcgcggc atgaaacagt aattcactaa aataaatgcc
     6541 gtgataaagc gtgttggtca ctaccagccc cagcgtctga gtactcttcg ccgacagatt
     6601 gcgggccagc aaatttgggc ggtaactgct ctcttctacc gcctgaaaca cgcgatcttt
     6661 agtctcctgg ctgacgtagc cattacctga aagcacgcgg gaaacggtcg cttttgaaac
     6721 cccggcgcgc ttcgccactt ccagcatcgt cgtcatcatt ttcatccctt tacacgcaat
     6781 caacgcagtg tactgcaccg tttgccgatt gtccttgcgc aatcggctcc agcggataag
     6841 acgaggtcgc cgtgccggtt ttgatgactt ttttgataaa ggtaaacatg gcgattcctt
     6901 atttcagcgg cgagttttta cgctcaatgc tgtaacgctc gagttctttg tacggcacca
     6961 ctttgctctt cttcttacgc acatcgacta cggtcatgcg gtcggtacag gagtagcaag
     7021 ggtccaggct accgataatc agcggcgcat aggaaacggt attgccacgc agcatgtagc
     7081 gcagggtcgg ccagttcgcg taggtcgcgg cacggcagcg ccagcggtac agcttctggt
     7141 tgtcgccggt catgctccag tggatatcgt cgccgcgcgg cgcttcggca aagcccagcg
     7201 cgaaacggtg cggaatgtag gtaaagcctt ccaccatcag cggaccgccc ggcaggttat
     7261 ccagaccgta gtcgatcata ttcagcgcgg tatagacctc gttgattcgc actttcagac
     7321 gggaaataac gtcgcagccc tgctcgctgt ggacttccat tggcagcagg ccataaccga
     7381 caaacgggtg atcggcgcga gtatcgcggg cgagaccgct ggcgcgaacc atcgggccaa
     7441 cgttactgaa atcgcgggcg atttccgggt ccagacgacc aatgccgacg gtgcgttgtt
     7501 ccatgttcgg cgtactcagc agcacatcca ccagctcctg cacttcacga cgcatttgtt
     7561 gcgccagctg gcgggtctgg atcatgtcat ctttcagcag atcgcgacga atcccgccga
     7621 tcaggttcag gccgtaggtt ttacgcgcac cggtaaggat ctctgccatt ttcatggagg
     7681 tttcacgcac gcggaagaac tgcataaagc cggagtcgaa gccggtaaag tgacaggcga
     7741 ggccgaggtt gagcagatgc gggtgcaggc gttccacctc cagcagaatg gcgcggatca
     7801 tctgcgcgcg ttctggcacc tgaataccca tcgcgttttc caccgacgtg gtgtaggcgg
     7861 tgctgtgggc aaagccgcag atcccgcaca cacggtcaga gaggaaggtc acttcgttat
     7921 aacccatgcg ggtttccgcc agtttttcca taccgcgatg gacgtagaac agacggtagt
     7981 cggcgtcgat aatgttttcg ccatcgacga acagacggaa gtgacccggt tcgtcagaag
     8041 tgacgtgcag cggaccaatc ggcacgacgt tgtttttctt gtcgcccagt tcgttgatga
     8101 actcgtaggt ttcagcatcg gtagtcggtg ccggacgctg acgataatcc atgctgtctt
     8161 tacgcagcgg ataaagttca tccggccagt catccggcag caccagacga cgttcatccg
     8221 gcagaccaac cggaatcaaa ccgtacatat cgcgcacttc gcgctcgccc cacaccgccg
     8281 ccggaacgcg cggcgtcacg gacggatact ccggtttgtt ggcgtcaact tcgacgcgaa
     8341 ccgttaccca acacttggtg cccttctcca tcgacagcac gtaataaacg gcgtaatgac
     8401 cattcagttt gcgttcgtcg ttaccaaaca gcaccgacag ccagccaccc tgcttgtagt
     8461 agagaaactc caccacttcc ggcagatagt tcacctttac ggtgacagtc agctgatctt
     8521 tggtctgcca ggcgtggtcc agcacgacgc ctagaaatgc ctcattcagc gcggcgagat
     8581 aatgttgacc taatttttct tcagacatgc tcaaactctc tttaatcacg ccgccagcaa
     8641 ggagacgaac gctaaaaatg caaagccaaa cccagcccag gtaatgcgcg gagtaatatc
     8701 aagacgcaga cgggccatgc tgttttcgaa cagcgcgata accaggacgc cgaccaccag
     8761 tttgacgatg gcaatcacca gcgccagcag cagtccaccg acggtgaagg tttccatttg
     8821 tccccacgga ataaacaccc cgacgaacat ctgcaacacc accagctgtt tcaggctgat
     8881 accccatttc atgacgccaa agccgctacc gctgtattcg gagagcggac cttcctgcaa
     8941 ctcctgctcg gcttccgcca ggtcgaacgg cagtttgccc atttcgataa aagtggcgaa
     9001 cgcacaggcg cagagcgcca gcaccagcgg gatgctttgg ctcagcggcc agtgataaac
     9061 ggtgtcggta atgttgctga tgttggtgga accggcaacc tgtgcggcaa cccacagacc
     9121 aagcagcaac atcggttcga ccagcacgcc aagcatcgct tcacggctcg caccgatagc
     9181 ggtaaacggg ctaccggtat ccagaccgga aatggcaaag aagaaacgcg cgatggcgaa
     9241 gagatacagt aaggtgatca aatcgcccag ttgcggcagc ggagaaccga cggtcaccac
     9301 cggcagcgca gtagcgatag tcagcatgac gcccaccatc acatacggcg tcaggcggaa
     9361 catccagcca gaggcatccg ggccaacgct ctggcggccc agcagtttga taatgtcgcg
     9421 atactcctgc aacacgcccg gcccgcgacg gttatgcaga cgggcgcgcg ccacgcgggt
     9481 aataccggag agcagcggcg caacggcaaa taacaccagc gcctgaatta acggatataa
     9541 aacactcatt ctcaggctcc tcgtgaaaca ataatcacca ccagtaccgc cagttcaacc
     9601 agcgccatcc ggcggaacag caacgcacta ccttcgcact gccagcccgg cactagcgac
     9661 accggattca gccatttgcg cagtttcagc actggcgcaa acgcctgttt aaccggcatg
     9721 gcaaaaccgt gagcggtaat caccattgat ttttcatggt cgtagccgca cacccaggcc
     9781 gcaccgcggg aacgtgacgg caggcggtcg cctttgcaaa tcgccataat gatgaatggc
     9841 agcagcgggc aggcaatcag cagcaatgtg atcattggtt gagaaacggt ggtgttagca
     9901 ggctccagag gcagaggtac agcagcagag agcatcggca gcagccacgg cgcagcaaca
     9961 ccgccaatta cgcagcaaat cgccagtgcc actacgctta cgctcatcag gagcggcgca
    10021 caggtggcgt tttcggcttc tttggtacgc ggcgcgccga ggaacgtgac gccatagact
    10081 ttcgccatac acatcaccgc cagcgcaccg gtaattgcca gacccacagc gagcagcggc
    10141 cccagcagac gggcaacaaa cgcgccgcta ttgctcagtt tgaagaagga ttgatagata
    10201 acccattccc cggcaaaccc gttcagtggc ggcagcgcag ccattgccat caacccgact
    10261 aacatggcga tggagataac cggcattttc ttgccaatac caccgagttt ttcgatatcg
    10321 cgatgaccgg tacggaacca gacgctcccc gccccgagga acagtacgct
//
LOCUS       DAZXUK010000136        10332 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZXUK010000136 DAZXUK010000000
VERSION     DAZXUK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10332
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.136"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1100)
                     /locus_tag="ACH3L8_002649"
     CDS             complement(<1..1100)
                     /locus_tag="ACH3L8_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFT4085706.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="ACH3L8_002650"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="ACH3L8_002650"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFT4085707.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="ACH3L8_002651"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="ACH3L8_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFT4085708.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="ACH3L8_002652"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="ACH3L8_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFT4085709.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="ACH3L8_002653"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="ACH3L8_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFT4085710.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="ACH3L8_002654"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="ACH3L8_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFT4085711.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="ACH3L8_002655"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="ACH3L8_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFT4085712.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="ACH3L8_002656"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="ACH3L8_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFT4085713.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="ACH3L8_002657"
     CDS             complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="ACH3L8_002657"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFT4085714.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="ACH3L8_002658"
     CDS             complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="ACH3L8_002658"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFT4085715.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="ACH3L8_002659"
     CDS             complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="ACH3L8_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFT4085716.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="ACH3L8_002660"
     CDS             complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="ACH3L8_002660"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFT4085717.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
BASE COUNT         2392 a         2763 c         2605 g         2572 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaataatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaaaaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgctctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgcactcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggtgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgtgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttgacgtcc agcagctcca gctgtttatt gccgtagcgt acccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtgtcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgcg cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc cagctcttcg
     8281 gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt agccggagaa
     8341 gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc acggtcgata
     8401 tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg atcaacggtt
     8461 tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa gcggttttca
     8521 atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt cattatgact
     8581 cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag agatccccgg
     8641 gctgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc attgcgctta
     8701 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
     8761 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
     8821 gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
     8881 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
     8941 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
     9001 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
     9061 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
     9121 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
     9181 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
     9241 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
     9301 tagtggtcag agtcgctgtt tttcagcgat atggtgctgc caagcggtga taaagccctc
     9361 gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc actgtacgcc
     9421 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
     9481 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
     9541 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
     9601 atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat gttccagcaa
     9661 ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca ccagttcttg
     9721 taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat taattatcgc
     9781 catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt caccatatag
     9841 gtgcggctgc acattgctgg gactactagg aagataaatg ccatcgagtt tcggcaaaag
     9901 ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca agccgcctgc
     9961 gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac ccttaagcct
    10021 gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca tgctcaatct
    10081 cacaaaatgg actaaattat cgccattact gcgctttaaa tgacctttat gttcaatatt
    10141 ttttcaatct agcagtggat tttcattttt gcaaactcaa tttaacattt gacaaacatt
    10201 tggtttgcat acagattcga atgatggtca ttatatttta cgctttgata acgagcggaa
    10261 aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga actttgttca
    10321 gcagtcagaa ga
//
LOCUS       DAZXUK010000137        10243 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZXUK010000137 DAZXUK010000000
VERSION     DAZXUK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10243
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.137"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1951
                     /gene="acnA"
                     /locus_tag="ACH3L8_002661"
     CDS             <1..1951
                     /gene="acnA"
                     /locus_tag="ACH3L8_002661"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFT4085718.1"
                     /translation="SMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKFVE
                     FYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEKYA
                     KAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELKVN
                     ATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLGLK
                     RQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIETA
                     IKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEPIGHD
                     RKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDTYGWQ
                     EDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAGRY
                     LQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVVSI
                     YDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLIG
                     MGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCRCR
                     IDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(2015..2605)
                     /gene="ribA"
                     /locus_tag="ACH3L8_002662"
     CDS             complement(2015..2605)
                     /gene="ribA"
                     /locus_tag="ACH3L8_002662"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFT4085719.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            2775..3539
                     /gene="pgpB"
                     /locus_tag="ACH3L8_002663"
     CDS             2775..3539
                     /gene="pgpB"
                     /locus_tag="ACH3L8_002663"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFT4085720.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            3688..3996
                     /gene="lapA"
                     /locus_tag="ACH3L8_002664"
     CDS             3688..3996
                     /gene="lapA"
                     /locus_tag="ACH3L8_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFT4085721.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            4003..5172
                     /gene="lapB"
                     /locus_tag="ACH3L8_002665"
     CDS             4003..5172
                     /gene="lapB"
                     /locus_tag="ACH3L8_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFT4085722.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            5365..6102
                     /gene="pyrF"
                     /locus_tag="ACH3L8_002666"
     CDS             5365..6102
                     /gene="pyrF"
                     /locus_tag="ACH3L8_002666"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFT4085723.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            6102..6428
                     /gene="yciH"
                     /locus_tag="ACH3L8_002667"
     CDS             6102..6428
                     /gene="yciH"
                     /locus_tag="ACH3L8_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFT4085724.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(6554..6772)
                     /gene="osmB"
                     /locus_tag="ACH3L8_002668"
     CDS             complement(6554..6772)
                     /gene="osmB"
                     /locus_tag="ACH3L8_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFT4085725.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(7041..7790)
                     /locus_tag="ACH3L8_002669"
     CDS             complement(7041..7790)
                     /locus_tag="ACH3L8_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFT4085726.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(7880..8053)
                     /gene="yciZ"
                     /locus_tag="ACH3L8_002670"
     CDS             complement(7880..8053)
                     /gene="yciZ"
                     /locus_tag="ACH3L8_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFT4085727.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            8091..8219
                     /gene="ymiD"
                     /locus_tag="ACH3L8_002671"
     CDS             8091..8219
                     /gene="ymiD"
                     /locus_tag="ACH3L8_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFT4085728.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            complement(8201..10175)
                     /gene="pdeR"
                     /locus_tag="ACH3L8_002672"
                     /pseudo
     CDS             complement(8201..10175)
                     /gene="pdeR"
                     /locus_tag="ACH3L8_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
BASE COUNT         2609 a         2422 c         2613 g         2599 t
ORIGIN      
        1 ttccatgctt atcccggacg tagtgggctt taaacttacc ggaaaattac gtgaaggtat
       61 taccgccaca gacctggttc tcactgttac ccaaatgctg cgcaaacatg gcgtggtggg
      121 gaaattcgtc gaattttatg gtgatggtct ggattcacta ccgttggcgg atcgcgccac
      181 cattgccaat atgtcgccag aatatggtgc cacctgtggc ttcttcccaa tcgatgcagt
      241 aaccctcgat tacatgcgtt taagcgggcg cagcgaagat caggtcgagt tggtcgaaaa
      301 atatgccaaa gcgcagggca tgtggcgtaa cccgggcgat gaaccgattt ttaccagtat
      361 gttagaactg gatatgaatg acgttgaagc gagcctggca gggccaaaac gcccacagga
      421 tcgcgttgca ctgcccgatg taccaaaagc atttgccgcc agtaacgaac tgaaagtgaa
      481 tgccacgcat aaagatcgcc tgccagtcga ttatgttatg aacggacatc agtatcagtt
      541 acctgatggc gctgtggtca ttgctgcgat aacctcgtgc accaacacct ctaacccaag
      601 tgtgctgatg gccgcaggct tgctggcgaa aaaagccgta actcttggcc tcaagcggca
      661 accgtgggtc aaagcgtcgt tggcaccggg ttcgaaagtc gtttctgatt atctggcaaa
      721 agcgaaactg acaccgtatc tcgacgaact ggggtttaac cttgtgggat acggttgtac
      781 cacctgtatt ggtaactctg ggccgctgcc cgatcctatc gaaacggcaa tcaaaaaagg
      841 cgatttaacc gtcggtgcgg tgctgtccgg caaccgtaac tttgaaggcc gtatccatcc
      901 gctggttaaa actaactggc tggcctcgcc accgctggtg gttgcctatg cgctggcggg
      961 aaatatgaat atcaacctgg cttctgagcc tatcggccat gatcgcaaag gcgagccggt
     1021 ttatctgaaa gatatctggc catcggcaca agaaattgcc cgtgcggtag aacaagtctc
     1081 cacagaaatg ttccgcaaag agtacgcaga agtttttgaa ggcacagcag agtggaagga
     1141 aattaacgtc acacgatccg atacctacgg ttggcaggag gactcaacct atattcgctt
     1201 atcgcctttc tttgatgaaa tgcaggcaac accagcacca gtggaagata ttcacggtgc
     1261 gcggatcctc gcaatgctgg gggattcagt caccactgac catatctctc cggcgggcag
     1321 tattaagccc gacagcccag cgggtcgata tctacaaggt cggggtgttg agcgaaaaga
     1381 ctttaactcc tacggttcgc ggcgtggtaa ccatgaagtg atgatgcgcg gcaccttcgc
     1441 caatattcgc atccgtaatg aaatggtgcc tggcgttgaa ggggggatga cgcggcattt
     1501 acctgacagc gacgtagtct ctatttatga tgctgcgatg cgctataagc aggagcaaac
     1561 gccgctggcg gtgattgccg ggaaagagta tggatcaggc tcaagccgtg actgggcggc
     1621 aaaaggacct cgtctgcttg gaattcgtgt ggtgattgcc gaatcgtttg aacgaattca
     1681 ccgttcgaat ttaattggca tgggcatcct gccgctggaa tttccgcaag gcgtgacgcg
     1741 taaaacgtta gggctaaccg gggaagagaa gattgatatt ggcgatctgc aaaatctaca
     1801 acctggcgcg acggttccgg tgacgcttac gcgcgcggat ggtagccagg aagtcgtacc
     1861 ctgccgttgt cgtatcgaca ccgcgacgga gttgacctac taccagaacg acggcatttt
     1921 gcattatgtc attcgtaata tgttgaagta acaagtattt gcttgccggt tattttgctt
     1981 ccggcaagca aatgaattac acgatgcaag agggttattt gttcagcaaa tggcccattt
     2041 tctcggcttt ggtatcgaga taatgttcgt tattggggtt acgacctaca atcaatggta
     2101 cgcgttcaac aatattaatc cctgcttcgg tcagaatttc gacttttttc gggttattgg
     2161 ttaacaagcg gacttcattg acgccaagga gtttgaacat atcagcgcaa agagtgaagt
     2221 cgcgctcatc agcggcgaag cctaactggt ggttagcctc tacggtatcg taaccttgat
     2281 cctgcagtgc gtaagcgcgg attttattca gcagaccaat gttacgacct tcctgacggt
     2341 gatacagcaa aataccacgg ccttcctcgg caatttgcgt caatgccgct tcgagctgga
     2401 agccacaatc gcagcgcaag ctgaacaggg cgtcaccggt cagacattcg gaatggacgc
     2461 gcgcaagtac cggggtatgc ccggaaatat cgccatagac tagcgcaaca tgatcgtgtc
     2521 cggttgccag ttcttcaaat cccaccatca ggaaatcgcc ccatggggtt ggcagtttgg
     2581 cttctgccac acgtttaagc tgcatgaaat tctccagata atgctggttc tgtattggct
     2641 tattttgcca taacgagaag gggttcacct aatcacgacg cgtcgatcgt tcacggaatg
     2701 gcacaaatct gtcaattttt cttggaactg gcattttcag ttatgattgt gggacttatc
     2761 aaaaaggaga ggccatgcgt tcgattgccc gacgtaccgc agtgggagct gcactattgc
     2821 ttgtcatgcc agtagccgta tggatttctg gctggcgttg gcaacctgga gaacaaagtt
     2881 ggctactaaa agcggctttt tgggttactg aaactgtcac ccagccctgg ggcgtcatta
     2941 cacatttgat tttgttcggc tggtttctct ggtgtctgcg ttttcgcatt aaggctgcct
     3001 ttgtattatt tgccattctg gcggcggcaa tccttgttgg acaaggcgtt aaatcctgga
     3061 tcaaagacaa agtacaggaa ccacgacctt ttgttatctg gttggaaaaa acacatcata
     3121 ttccggttga tgagttctac actttaaagc gagcagaacg cggaaatcta gtaaaagaac
     3181 agttggctga agagaaaaat attccacaat atttgcgttc acactggcag aaagagacgg
     3241 ggtttgcctt tccttccggt cacacgatgt ttgctgccag ttgggcactg ctggccgttg
     3301 gtttgctgtg gccgcgtcgg cgaacgttaa ccattgctat cttgttggtc tgggcaacgg
     3361 gagtcatggg aagccgcctg ctgctcggga tgcattggcc acgcgatctg gtggtagcta
     3421 cgttgatttc gtgggcgctg gtggcggtgg caacgtggct tgcgcaacga atttgtgggc
     3481 cattaacacc acctgcggaa gaaaatcgcg aaattgcaca acgagaacaa gaaagttaac
     3541 gctggttgat tttccgaatt taggccttaa atcatcaaca atgcgcgtgg atgccatttc
     3601 gcagacggcg cgaaaatggt actttaaagg gctattgcgg taagttgacc ataatttatt
     3661 cgctctaacc acataacggg aagtaatgtg aaatatttac tcattttctt actggtgtta
     3721 gcgatcttcg tgatttcggt cacgttgggt gcgcagaacg atcaacaggt gacgtttaat
     3781 tatctgttag cgcaagggga gtaccgtatt tccacattgc tggcggtatt gtttgctgcg
     3841 gggtttgcta tcggttggtt gatttgtggc ctgttctggc tgcgagttcg tgtttccctg
     3901 gcgcgcgctg aacgtaaaat aaagcgactg gaaaaccagc tttcacccgc gactgacgtg
     3961 gctgtagtgc cgcactcgtc agcggcgaag gaataacttt ctatgctgga gttgttgttt
     4021 ctgcttttgc ctgtagccgc tgcctatggc tggtatatgg gccgcagaag tgcgcaacaa
     4081 aacaagcaag atgaagccaa ccgcttgtcg cgtgattacg tagcgggggt taacttcctg
     4141 cttagtaatc aacaggataa agcggtagac ctgtttctcg atatgcttaa agaggataca
     4201 ggcaccgttg aagcccacct tacgctcgga aacctgttcc gttcgcgtgg cgaagttgat
     4261 cgcgctattc gcatccatca gaccctaatg gaaagcgcct cgctgaccta tgaacagcgt
     4321 ctgttggcga ttcaacaact ggggcgtgat tacatggccg ccgggttata cgaccgcgcg
     4381 gaagacatgt tcaatcagct gaccgatgaa actgacttcc gcattggcgc gctgcaacag
     4441 ttgctacaaa tctaccaggc taccagtgag tggcagaaag caattgatgt tgccgaacgc
     4501 ctggtgaagc tgggtaaaga taaacagcgc gtcgaaattg cccatttcta ctgtgagtta
     4561 gccctgcagc atatggccag cgacgatctc gatcgtgcca tgaccttgct aaaaaaaggg
     4621 gcggcggcag ataaaaacag cgcccgcgta tccatcatga tgggacgcgt gtttatggcg
     4681 aaaggagaat acgccaaagc cgtcgaaagt ttgcaacgcg tcatatccca ggacagagaa
     4741 ctggtcagcg aaacgctgga aatgttgcaa acctgctatc agcagttggg taaaactgcc
     4801 gaatgggcag aattcctgca acgcgcggtg gaagagaaca ccggtgccga tgctgaattg
     4861 atgcttgctg atatcatcga agcgcgcgac ggtagtgagg ctgcacaggt ctatattaca
     4921 cgccagcttc agcgtcatcc gaccatgcgt gtattccata agctaatgga ttaccactta
     4981 aatgaagcgg aagaagggcg cgccaaagag agtctgatgg tgctgcgtga catggttggc
     5041 gagaaggtgc gtagtaagcc tcgttatagc tgccagaaat gtggttttac cgcatacacc
     5101 ctctactggc attgtccgtc ttgtcgtgcc tggtcaacta ttaaaccgat tcgcggtctt
     5161 gatggcctgt aatttttaaa aaaatccgac tttagttaca acatactagt tattgatgtc
     5221 ccattgtgct ccggcaacga cggagcagaa aaagcctgct aggggaaaaa tcgcaactgt
     5281 taatttttta ttttcacggg tagaatgctc gccgtttacc tgtttcgcgc cactcccggt
     5341 gcccatcatc aagaaggtct ggtcatgacg ttaactgctt catcttcttc ccgcgccgtt
     5401 acgaattctc ctgtggttgt tgcccttgat tatcataatt gtgatgacgc gctgtccttt
     5461 gtcgacaaga tcgacccacg cgattgtcgt ctgaaggtcg gtaaagagat gtttacattg
     5521 tttgggccac agtttgtgcg cgaacttcaa cagcgtggtt ttgatatctt tcttgacctg
     5581 aaattccacg atatccccaa cactgcagcg cacgctgtcg ctgctgcagc tgacttaggc
     5641 gtgtggatgg tgaatgttca tgcctccggt ggggcgcgta tgatgaccgc agcgcgtgag
     5701 gcactggttc cgtttggcaa agatgcaccg cttttgattg ctgtgacagt gttgaccagc
     5761 atggaagcca gcgacctggc cgatcttggc gtgacactgt cacctgcaga ttatgcagaa
     5821 cgtctggcag cactgacgca aaaatgtgac cttgatggtg tggtgtgttc tgctcaggaa
     5881 gctgtgcgct ttaaacaggt attcggtcag gagttcaaac tggttacacc gggcattcgt
     5941 ccgcagggga gtgaagctgg tgaccagcgc cgcattatga cgccagaaca ggcgttggcg
     6001 gctggtgttg attatatggt gattggtcgc ccggtaacgc aatcggtaga tccagcgcag
     6061 acactgaaag cgatcaacgc ctctttacag cggagtgcat gatgagtgat tccaacagcc
     6121 gtctggtcta ctcaacggaa accgggcgta ttgatgagcc caaagcggcc ccagtacgcc
     6181 ctaaaggcga tggagcggtg cgtattcagc gtcagaccag tggacgtaaa ggtaagggcg
     6241 tttgcctgat taccggtatc gatctcgatg atgccgaact gacaaaactt gcagcggaac
     6301 tgaagaaaaa atgcggctgc ggcggagcag ttaaagatgg agttattgaa atccagggcg
     6361 ataagcgtga tttattaaaa tcactacttg aagcgaaagg gatgaaggta aaactcgcag
     6421 gcggttaaca taaaaagcca cggatatatc cgtggctttc gaatatttta ctgtgtgtat
     6481 tattcagtta tttcgtactg aacagaatca agcgtattat aattgcgagt cgataatcga
     6541 catttattta cgattattta ccgacctggt gaccaataac accaccgaca gctgcaccac
     6601 ctaatgtacc caacgtactg ccatcggtca gtactgcacc gcctaatgcc cctgcacccg
     6661 cgccgattgc ggtgttgcgg tcccgtttag accagttaga acaggcactc agagacattg
     6721 ccaaagtaat tgccagaaca gccgcggtca tttttttgct cgttacaaac ataatactct
     6781 ctcctgaatt tatgattcac ggaagtaagc tctctataac tataatagct aagaataagc
     6841 ctggtgaaat tattccgtga aatctgctcg cgcgcagggt tatatcacgc aggtgataat
     6901 tacttcctgt tatatcgctg ataataattt tatatcttga gagtgttaat aacaggtaaa
     6961 tagtcttaat tatcaaccag gaatcatctt agagcggatg atttgccaaa ctgcaaatca
     7021 tccgtagaga agggaaatgg ttaaatgtca acgacgtgaa cggtgagttt actgtgctca
     7081 agatgcatca gatcgctggc gcgtattttc gaatcggtaa tcacgcgatt gaatcgctca
     7141 acgggaccga tggagtatga atgtacagca ccaaatttcg agctgtcagt caggactatc
     7201 gcttcgcact ctttttccag cacggcattg accacatcgg tgcgcatcat atcgcgaccg
     7261 gtgaatccag tttcaggttg ccagccatca ataccaataa atgctttgct gaaatgtacc
     7321 tgctggatgc actggcgtgt caaagggcca accatacttt cgcttttttt ctggtacacg
     7381 ccaccgagca aaatgacttc acaaggcgcg tctttcagca aatgcgcgat gtagctgctg
     7441 accgtgatga tagtgacatt tttcttctgc tcccccagag tccgggcgag cagggcattg
     7501 ctgctgccat tttcgataaa gatggtttcg cccggttgaa ccagtgacgc ggcaaactcg
     7561 gcgagttcac gcttcagcgt atagttgctc atcatacggg tttcgacgtc atcactatca
     7621 agcgaaactg caaagccatg tgcacggcgg aggtaactca gtttttcgag ggtgttgaga
     7681 tcctggcgaa tggtaacttc agaaactcca gtggcttttg ccagatcggt tacgctaacc
     7741 tgaccctggt caatgaccat ctgtagaata gtttgttgtc gggaattcat cgctgtaatt
     7801 cttataacgt tataatacta aataaaaaaa tactgacgcg ccgggcatcc tcaaatagga
     7861 aaacctgaag gcacgattgt taaatctccc acggtgtctt cgggatgtct ggcgtagatt
     7921 cagcaacctg gcgcaagagt tctgctttaa gtatctcgag attatggatc gcagagtggt
     7981 aatcgcctgc cacaaggata tctaacacaa tatcaatacg ttcggcaacg cgctgggcat
     8041 caaattcaga catcaaggac atccttatag caaaaggggg aaaatgaata atgcaaaaat
     8101 tgccgctaaa agagaagtgt ttaacagcaa cggctaatta tcatccagga atacgatata
     8161 taatgacggg atatagcgct aagtatatat attcatctac ttatgcgcgc ttcagatagc
     8221 gtttatacca gcgttcgaag gcgacggcgg gcatcggttt ggcaaacaaa aatccttgcc
     8281 gctcattgat cccgttcttg gttaaaaaag catcttcctt tgcactctct acaccttcgg
     8341 cgatcacctg aagattcaat gcctgggcca cagcgacgat cgcccggacc agtgactgcg
     8401 agacaggttg tttgtgaata tctcgaacaa aaacctggtc aagtttgatg gcatcgatcg
     8461 gaaagcgcgc cagttgcgaa agtgaagagt agccggtacc aaaatcatcc agatgcactt
     8521 gcgcacctag ttggctaaat tgttgaataa cagacagtgc cagttcatca ttctcaatca
     8581 gacaactctc tgtcagttca acatctatag ggcagtattc aaaattgagt tcctggagaa
     8641 cctgtttcag ggcggtgaaa atggtttgat cggcgagctg acgtgcagaa atatttaccg
     8701 ccactcgcag gtttatgcct ttatcccgcc actttgccac ctggcgtacg acatcgagaa
     8761 tcacccaacg gcctaaaggc acaattagcc ctgactcttc ggcgtaggaa atgaagtcca
     8821 acggtggaat caacccacgt tcaggtgact gccaacgtac tagtgcttcc agactgcgca
     8881 cttcgacacg ctaggtgatt ttcgataacc aactgatcgt tttccagtgc tttacgcaag
     8941 ttggtatcca gccagagata ttcaaatacc cgctgattca tttctggagt aaaaacgcaa
     9001 aattgtcctc gtccgccttc cttcgctgtg tacattgctg tgtcggcgtg acgaataata
     9061 gtcgcgctgt ccgaaccatg ttcgggagag agcgaaatac ctactgaaca gctggtataa
     9121 acttcaatta aaccaatacg aaaggggagg cgtaagcggg tcaaaattcg tgatgccatt
     9181 gcttccagcg cgctttgtga ggtattggat gccagtacca gaaactcatc gccacctgga
     9241 cgcgccaaca cctggtcatg ttcgagacag cttaaaatag ccaatgacac gtcgcgtaat
     9301 aactggtcac caaacaaatg cccataggcg tcgttgacct ttttaaaatt atccaaatca
     9361 agatacacaa ccccaacttt attgttatct gcctgattaa tagcgtgatc gattaactcc
     9421 tgcattgcgt tacgattcgg cagtccggtg atactgtcgg tatttgccag aatacgcagt
     9481 cgctcctgag tgcggcgctc ttcggtaatg tcggtaccgg aacagattaa aaaaatctcg
     9541 tttttgccac tgccgctgtg gacaaattta ttgcgaaaca gaaacagccg ctggccttta
     9601 cgtgttggta tccacagttc gacttcatat gcattgccgc ttcgaaaaaa tacacggtta
     9661 ttgcgcctgg atgccgcagc ttcacgacgg ctcataaaca gtttaaacac gctttaccca
     9721 atgacgtcgt gttctttcaa ccctgtgtaa tcttcacata accgattgaa gcgttggata
     9781 ttcccccggc tgtcgagaat cactattgcg gaatgtgctt cagaaactac ctgctcagca
     9841 aaggaaagcc cgtgtgataa atcacgagca accgccggtg tgtcatgcca tgcagacgcg
     9901 ttgccagccc attcccgttt attgattttt cgtcctacca gatgcacaga aagatcgctt
     9961 tcatcgacgg tcagactcat catcaggctg gaggtgatga ccgtcatttc cctgatgcga
    10021 gcagcctgtt cggcagataa ctgcaatgta cgatcaggtt ctgtggtttt ggtggtagaa
    10081 aagcgcaaag catcgctgct ttccgtcaac cgccagtatg gattgtgcga tccgagaaag
    10141 ttgtacaacg ttgtggactc cctaacggtt ttcatgatgc atatcctccg gttaacagcg
    10201 gagtccgcgg ttaaataaaa ggaacaacat gacttacatg aaa
//
LOCUS       DAZXUK010000138        10239 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZXUK010000138 DAZXUK010000000
VERSION     DAZXUK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10239
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.138"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            72..1715
                     /gene="sapA"
                     /locus_tag="ACH3L8_002673"
     CDS             72..1715
                     /gene="sapA"
                     /locus_tag="ACH3L8_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFT4085729.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            1712..2677
                     /gene="sapB"
                     /locus_tag="ACH3L8_002674"
     CDS             1712..2677
                     /gene="sapB"
                     /locus_tag="ACH3L8_002674"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFT4085730.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            2664..3554
                     /gene="sapC"
                     /locus_tag="ACH3L8_002675"
     CDS             2664..3554
                     /gene="sapC"
                     /locus_tag="ACH3L8_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFT4085731.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            3554..4546
                     /gene="sapD"
                     /locus_tag="ACH3L8_002676"
     CDS             3554..4546
                     /gene="sapD"
                     /locus_tag="ACH3L8_002676"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFT4085732.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            4548..5354
                     /gene="sapF"
                     /locus_tag="ACH3L8_002677"
     CDS             4548..5354
                     /gene="sapF"
                     /locus_tag="ACH3L8_002677"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFT4085733.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            5422..5775
                     /locus_tag="ACH3L8_002678"
     CDS             5422..5775
                     /locus_tag="ACH3L8_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFT4085734.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            6143..6931
                     /gene="fabI"
                     /locus_tag="ACH3L8_002679"
     CDS             6143..6931
                     /gene="fabI"
                     /locus_tag="ACH3L8_002679"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFT4085735.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTRESFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            7075..8202
                     /locus_tag="ACH3L8_002680"
     CDS             7075..8202
                     /locus_tag="ACH3L8_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFT4085736.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            8270..10204
                     /gene="rnb"
                     /locus_tag="ACH3L8_002681"
     CDS             8270..10204
                     /gene="rnb"
                     /locus_tag="ACH3L8_002681"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFT4085737.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
BASE COUNT         2331 a         2679 c         2683 g         2546 t
ORIGIN      
        1 acacaaacgg taatggcaaa tgtcattaaa tattagtaag atagcctctt tacataaaat
       61 cccttaatat tatgcgccag gtattatcgt ctcttttggt gattgctgga cttgtgagtg
      121 gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga tatccgcgac agcggttttg
      181 tctattgcgt cagcgggcaa gtcaacacct ttaacccatc caaagcgagc agtgggttaa
      241 ttgtcgatac ccttgccgcc cagttttatg atcgactgct ggatgtcgat ccctatacct
      301 atcgcctgat gccggaactt gccgaaagct gggaagtact cgacaacggc gcgacctatc
      361 gcttccacct gcgtcgcgat gttccgtttc aaaaaaccga ctggtttact cccactcgta
      421 aaatgaatgc cgacgatgtg gtgtttacct tccagcgaat ttttgaccgc aacaacccgt
      481 ggcataacgt caacggcagc aacttcccct acttcgacag cctgcaattt gccgataacg
      541 tgaaaagcgt ccgcaaactg gataatcata ccgttgagtt ccgactcgcc cagccggatg
      601 cttctttttt gtggcacctc gcaacccatt atgcttcggt catgtcggca gaatatgccc
      661 ggaagttaga gaaagaagat cgccaggagc aactcgaccg tcaaccggtc ggcaccggtc
      721 cgtatcagtt gtcggaatac cgcgccgggc aatttattcg cctacaacgt catgatgact
      781 tctggcgcgg taaaccgtta atgccgcagg tagtggtgga tttaggctcc ggcggcaccg
      841 gacgtctgtc gaaactcctg accggggaat gcgacgttct ggcctggcct gctgccagcc
      901 agctatccat tttgcgtgac gacccgcgct tgcgtttaac gctgcgtcct gggatgaacg
      961 tcgcctatct ggcatttaac accgccaaac cgccgctaaa taatcccgct gtccgccatg
     1021 cactggcact ggcgattaat aaccagcgcc tgatgcaatc catctattat ggtacggctg
     1081 aaacggcggc ctctatttta ccgcgcgcct cgtgggccta tgacaacgag gctaaaatta
     1141 ctgaatacaa tccggcgaaa tcgcgcgaac agttgaaggc gttggggctg gaaaacttaa
     1201 cgctgaaact gtgggtgccc acacgttcgc aggcgtggaa ccccagtcca ctgaaaactg
     1261 ccgaactgat tcaggcggat atggcgcagg ttggcgtaaa agtggtgatt gtgccggtag
     1321 aaggtcgctt tcaggaggcg cggttgatgg atatgagcca tgatctgacg ttatccggtt
     1381 gggcgacgga cagtaacgac ccggacagtt tcttccgtcc gttactgagc tgcgcggcaa
     1441 ttcattcaca gaccaacctc gcccactggt gcaatccgaa attcgacagc gtgttgcgta
     1501 aggcgctctc ctcgcagcag ctggcagcgc gtattgaagc ctatgacgaa gcgcagagta
     1561 ttctggcgca ggaattacct attctgccgc tggcgtcgtc attgcgtttg caggcctatc
     1621 ggtacgatat caaaggtctg gtacttagcc cgtttggtaa cgcctccttt gctggggtgt
     1681 atcgcgagaa acaggatgag gtgaaaaaac catgattatc ttcaccttac gccgcatttt
     1741 gttattgatt gtcaccttgt tcctgctgac cttcgttggc ttcagcttga gctatttcac
     1801 tccacacgcg ccgttacaag gcgcgtcact gtggaatgcc tgggtgttct ggtttaatgg
     1861 cctgatccac tgggattttg gtgtttccag cattaacggt caaccaattg ccgaacagtt
     1921 aaaagagctc ttcccggcga cgatagaact gtgcatcctc gccttcggtt ttgcgctgat
     1981 tgttggcatc cccgtgggga tgattgccgg gattacgcgc cataagtggc aggataactt
     2041 gatcaacgcc atcgccttac tgggcttttc aatcccggtg ttctggctgg cgcttctgtt
     2101 gacactgttt tgttcactca cgctaggttg gttgcccgtt tcagggcgtt tcgatctgct
     2161 ctacgaagtg aaaccgatta ccggttttgc gttgattgat gcctggcttt cggactcacc
     2221 atggcgggat gaaatgatca tgagcgcaat ccgccatatg atattgcccg tgatcaccct
     2281 gtcggttgcg ccaacaactg aagtgatccg cctgatgcgt atcagcacta ttgaagtgta
     2341 tgaccagaac tatgtaaaag cggcggcgac ccgcggtttg tcgcgcttta ctattttgcg
     2401 tcgccacgtt ctgcataacg cgttaccgcc ggttattcct cgcctgggct tacagttttc
     2461 caccatgctg acgctggcga tgatcaccga aatggttttt agctggccgg gtttagggcg
     2521 ctggttaatt aacgccatcc gccagcagga ttacgcagcc atttccgccg gagtgatggt
     2581 gtgtggctca ctggtcatta ttgttaacgt gatttctgat attttgggtg ccatggctaa
     2641 ccctctgaaa cataaggaat ggtatgcctt acgatagcgt atacagcgaa aagcgcccgc
     2701 cgggcacgct gcgtaccgcc tggcgcaaat tttatagtga cgcctctgcg atggtcggcc
     2761 tgtacggctg cgcgggactg gctgtactgt gtatttttgg cggctggttt gcgccttacg
     2821 gcatcgacca gcaatttctc ggttatcaat tattgccgcc atcatggtcg cgctatggcg
     2881 aagtttcttt cttcctgggg actgacgacc tcggacgtga tgttttaagc cgtttactga
     2941 gcggagctgc gccaaccgtg ggtggcgcat ttgtggtgac gcttgccgcg acgatctgtg
     3001 gcctggtact gggcaccttc gccggggcga cacacggcct ccgctcagcg gtgcttaacc
     3061 atattctgga taccttactg gcgatccctt cgctgctgtt ggcaattatc gttgtagcgt
     3121 ttgccggacc gagtttgtcc cacgctatgt ttgctgtctg gctggcattg ctgccgcgta
     3181 tggtacgttc gatttacagc atggtgcatg acgaactgga aaaagagtac gttatcgccg
     3241 cccgtctgga tggcgcatca acgctgaata ttctctggtt tgctgtgatg ccaaacatca
     3301 ccgccgggct ggtcaccgag atcacccgcg cactgtcgat ggcaattctc gatatcgccg
     3361 cgctgggctt tctcgatctc ggcgcacaac tcccctcgcc tgaatgggga gcgatgctcg
     3421 gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt catgctgcca ggtgcggcaa
     3481 ttatgattag cgtgttgttg gttaacctcc ttggcgatgg cgttcgtcgt gcgattattg
     3541 cgggggtgga ataatgccat tactcgatat tcgtaacctg accattgaat ttaaaaccgg
     3601 tgatgagtgg gttaaagccg tcgaccgggt aagtatgacc ttaaccgaag gtgaaatccg
     3661 cggtcttgtt ggtgaatccg gttccggcaa gagtttgatt gcgaaagcga tttgtggggt
     3721 gaataaagat aactggcgtg ttactgctga ccgtatgcgt tttgatgata tcgatttgct
     3781 gcgtctctcc gcacgcgaac ggcgcaaact ggttggccat aacgtgtcga tgattttcca
     3841 ggaaccgcag tcgtgtcttg acccttcaga acgtgtgggt cgccagttga tgcaaaacat
     3901 cccagcctgg acctataaag gccgttggtg gcagcgtttt ggctggcgca aacgccgtgc
     3961 cattgaactg ctgcaccgcg tggggattaa agatcacaaa gatgcgatgc gcagttttcc
     4021 ctatgagttg accgaaggtg aatgtcagaa agtgatgata gccattgcac tggcgaatca
     4081 accgcgtctg ctgattgctg acgaaccgac aaactcaatg gagccaacaa cccaggcgca
     4141 aatctttcgc ctgctgacgc gtctcaacca aaacagcaat accactattt tgcttatcag
     4201 ccatgactta caaatgctta gccaatgggc ggataaaatt aacgtgcttt actgcggtca
     4261 aacagtggaa accgcgccga gtaaggagtt ggtgacgatg ccacatcatc cttataccca
     4321 ggcgctgatc cgcgcgatac cagacttcgg cagcgcgatg ccgcataaaa gtcgcctcaa
     4381 tactctaccc ggcgctatcc cattgctgga acagttaccg attggttgtc gtctggggcc
     4441 acgttgcccg tatgcacaac gagaatgcat tgtgacgcca cgtttgacgg gggcgaaaaa
     4501 tcatctctat gcctgtcatt tcccgctgaa catggagaaa gagtgagatg atcgaaacgc
     4561 tgcttgaagt gcgtaattta agtaaaacgt tccgctaccg gaccggttgg tttcgtcgtc
     4621 agaccgtaga agcggtaaaa cccttgagct ttacgctacg tgaaggccag acactggcga
     4681 ttattggcga gaatggttcg ggtaaatcca cactggcaaa aatgctggcg ggaatgatag
     4741 aacctaccag cggcgagtta ttgattgacg atcatccgct gcattttggc gattattcct
     4801 tccgtagtca gcgtattcgc atgatttttc aggatccttc gacctcgttg aatccccgtc
     4861 aacgtatctc acaaattctg gattttccac tgcgcctgaa caccgacctg gaaccggaac
     4921 agcgtcgcaa acagattatt gaaacgatgc gtatggttgg gctgctaccg gatcacgtca
     4981 gttactatcc gcatatgttg gcacccggac aaaaacagcg tctgggtctg gcgcgcgcgc
     5041 tgatattgcg cccaaaagtc attattgccg atgaagccct cgcttcactg gatatgtcaa
     5101 tgcgttcgca gttgattaat ctgatgctgg agttacagga aaaacagggc atttcgtata
     5161 tttatgttac ccagcatatc ggaatgatga agcacattag cgaccaggtg ctggtgatgc
     5221 atcagggcga ggttgtcgag cgaggcagca ccgcggatgt gctggcatcg ccgctgcatg
     5281 aactcaccaa acggctgatt gccgggcact ttggcgaagc attgacagcg gatgcgtggc
     5341 gtaaagatcg ctaattttct gaatcctcct tccagattat tcacctcatt ctgacaagag
     5401 attaaatctt gtcagaatga ggtgatggat aaaataaaat caaatgcgcg tgatttaaga
     5461 cgcaatctca ctttgcagga acgaaagctc tggcgatatc ttcgcagccg acgttttggt
     5521 gatttcaaat ttcgccgtca acatccggtg gggagctaca ttctcgattt tgcttgctgc
     5581 tcggcgcgcg tagtcgttga gctggatggt gggcagcatg atttggcagt tgcctatgat
     5641 accaggcgca ctagctggct tgagtcgcag ggctggaccg tgctgcgttt ctggaataac
     5701 gagattgatt gtaatgagga ggcggtgctg gagattattc tgcaggaact gaaccgccgg
     5761 tcaccctcac cctgaaaggg cgagggggcc gaccgagccg aatagctgtt gtggtgaaaa
     5821 catggagaca gtgctggaga atattcggca aggtctgaac catcccagcc atcgccataa
     5881 aagggttagg ggctgtatga gcctgtttgt tgctggggta acaatatttg cacaatatgg
     5941 tcccctcgcc cctctgggga gagggttagg gtaaggggaa aagcgccccc cctgccgcag
     6001 cctgctccgg tcggacctgg taactatagc tactcacagc caggttgatt ataataaccg
     6061 tttatctgtt cgtactgttt actaaaacga cgaatcgcct gattttcagg cacaacaagc
     6121 atcaacaata aggattaaag ctatgggttt tctttccggt aagcgcattc tggtaaccgg
     6181 tgttgccagc aaactgtcca tcgcctacgg tatcgctcag gcgatgcacc gcgaaggagc
     6241 tgaactggca ttcacctacc agaacgacaa actgaaaggc cgcgtagaag aatttgccgc
     6301 tcaattgggt tctgacatcg ttctgcagtg cgatgttgca gaagatgcca gcatcgacac
     6361 catgttcgct gaactgggga aagtttggcc gaaatttgac ggtttcgtac actctattgg
     6421 ttttgcacct ggcgatcagc tggatggtga ctatgttaac gccgttaccc gtgaaagctt
     6481 caaaattgcc cacgacatca gctcctacag cttcgttgca atggcaaaag cttgccgctc
     6541 catgctgaat ccgggttctg ccctgctgac cctttcctac cttggcgctg agcgcgctat
     6601 cccgaactac aacgttatgg gtctggcaaa agcgtctctg gaagcgaacg tgcgctatat
     6661 ggcgaacgcg atgggtccgg aaggtgtgcg tgttaacgcc atctctgctg gtccgatccg
     6721 tactctggcg gcctccggta tcaaagactt ccgcaaaatg ctggctcatt gcgaagccgt
     6781 taccccgatt cgccgtacag ttactattga agatgtgggt aactctgcgg cattcctgtg
     6841 ctccgatctc tctgccggta tctccggtga agtagtccac gttgacggcg gtttcagcat
     6901 cgctgcaatg aacgaactcg aactgaaata atcgttctgt tggtaaagat gggcggcgtt
     6961 ctgccgcccg ttatctctgt tatacctttc tgatatttgt tatcgccgat ccgtctttct
     7021 ccccttcccg ccttgcgtca ggataacgat ttcctttacg accaaggagc gcccatggaa
     7081 caacgccaca tcaccggcaa aagccactgg tatcatgaaa cgcaatccag tactacggag
     7141 tatgacgttc tgcctctggt cccggaagcc gcaaaggtca gcgatccctt tctgctcgac
     7201 gtgatccttg atgaagagac gctggccccc ttcctttcat ggctggtccc tgcgcgcgtt
     7261 cttgcagtgg agttgttccc tgaccagctt accgtgaccc gttcacaaac cttcaccgct
     7321 tatgaacgct tgtcgacggc cctgacggtt gctcaggttt gcggcgtcca gcggttatgt
     7381 aactactatt cggcgcgact tacgccgctc cccggacctg attcctccag ggaaagtaat
     7441 catcggttgg cacaaatcac gcaatatgcc cgccaactgg ctagctcgcc ttctattatc
     7501 gacaaccgat cgcgccagca tctgaatgac gtcggtctta ctgtctggga ctgtgtgatc
     7561 attaaccaaa tcattggttt tattggcttt caggcgcgga caattgcgac atttcaggct
     7621 tatctcgggc atccggtacg ctggttaccc gggctggcga tacaaaacta cgccgacgcg
     7681 tcactgtttg ctgatgaatc aatacgctgg cgaagcagct atgaggtgga aaaactacct
     7741 gaagagcaca caaaaagttc aactgcagaa ctttgccaac tggccgaaac actctctctc
     7801 caccctattt cactttccct tctcgaaaag ttgttaaaca gcacacgggt caatgcacag
     7861 ccggataatc agcttgcggc gttgttatgc gcgcgtataa atggcagtcc tgcttgtttt
     7921 tccacctgta tggattcatc aaatgaatat aaaaaaatca gcacccttct gcgcaagggc
     7981 gaaaatgaaa ttaaccgatg ggctgaccgt cattctgttg agcgcgctac cgttcaggcg
     8041 atacaatggc tgacccgagc acccgatcgc tttagcgccg cccagttcag ccctttactc
     8101 gaacacgaaa aatcatcaac gcagattatt aatctgctgg tatggagcgg gctgtgtggc
     8161 tggataaatc gcttaaaaat cgcgttgggt gagacatatt aaccttgccg cgtcagacag
     8221 attcgcgtaa aactgtcagc cgctctaatg gccaccaaaa tagacaatta tgtttcagga
     8281 caacccgctg ctagcgcagc ttaaacagca actgcattcc cagacgccac gcgctgaagg
     8341 ggtggtaaaa gccacagaaa aaggctttgg cttcctggaa gtcgacgcac aaaaaagtta
     8401 tttcattccg ccgccgcaga tgaaaaaagt catgcatggc gaccgaatta tcgcggtgat
     8461 ccacagtgaa aaagaacgtg aatccgcaga gccagaagaa ctggttgaac cgttcctgac
     8521 tcgtttcgtg ggtaaagttc agggcaaaaa tgaccgtctg accatcgttc ctgatcatcc
     8581 acttttaaaa gacgccattc cttgccgcgc agcccgtggc ctgaaccacg agtttaaaga
     8641 aggcgactgg gcggttgccg aaatgcgccg tcatccgctg aaaggcgatc gttctttcta
     8701 tgctgaactg acacaataca tcacttttgg tgacgaccac tttgtaccgt ggtgggttac
     8761 ccttgcgcgc cataatctgg aaaaagaagc accagacggc gtcgctaccg aaatgctcga
     8821 tgaaggtctg gttcgtgaag atctgacctc gctggatttt gtcaccatcg acagtgccag
     8881 cactgaagat atggatgacg cccttttcgc taaggcgctg ccggatgaca aacttcagct
     8941 gattgtggcg attgccgatc caaccgcgtg gattgctgaa ggcagcaagc tggacaaagc
     9001 cgcgaaaatt cgcgcattca ccaactatct gcctggcttc aacatcccta tgctgcctcg
     9061 cgagctttct gacgatctct gctcactgcg cgccaatgaa gtccgcccgg tactggcatg
     9121 ccgcatgacg ctctccgctg atggcaccat tgaagataat atcgaattct ttgccgccac
     9181 catcgaatcc aaagcgaagc tggtgtatga ccaggtttct gactggctgg aaaataccgg
     9241 tgactggaag cctgaaagtg aagcaattgc cgaacaagtc cgtttgctag cgcaaatttg
     9301 ccaacgccgc ggcgagtggc gtcataacca cgcactggtg tttaaagatc gcccggatta
     9361 ccgctttatt ctcggtgaaa aaggtgaagt gctggatatc gtcgccgagc ctcgtcgcat
     9421 tgccaaccgt atcgtcgaag aagcgatgat tgccgctaac atttgtgcgg cccgcgtact
     9481 gcgcgataag ctcggttttg gcatctataa cgtgcatatg ggctttgatc cggcgaatgc
     9541 cgacgcgctg gcagcgttgc tgaaaacgca cggtctgcat gtcgatgccg aagaagtgct
     9601 cacgctggac ggtttctgca aactgcgtcg tgaactggat gcgcaaccaa ctggtttcct
     9661 cgacagccgc attcgtcgct tccagtcatt tgctgaaatt agctctgaac ccggtcctca
     9721 ctttggcctc ggtctggaag catacgccac ctggacttcg ccgatccgta aatatggcga
     9781 catgatcaac caccgtctgc tgaaagcggt tatcaaaggc gaaactgcga cgcgtccgca
     9841 ggatgaaatc actgtccaaa tggccgaacg tcgccgtctc aaccggatgg cagaacgtga
     9901 tgttggcgac tggttatacg cacgcttcct gaaagataaa gccgggaccg gcacccgttt
     9961 cgcggcggaa attgtcgata tcagccgtgg cggcatgcgt gtgcgtttgg ttgataacgg
    10021 cgctatcgcc tttattccgg cacctttctt acacgctgtg cgcgatgaac tggtttgcag
    10081 ccaggaaaac ggcactgtac aaattaaagg tgaaacggtt tacaaagtaa ctgacgttat
    10141 tgacgtcacc attgccgaag tccgcatgga aacccgcagc attattgcgc gcccggtcgc
    10201 gtaatctcct ttcacggccc attcctcatg gatgggccg
//
LOCUS       DAZXUK010000139        10027 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZXUK010000139 DAZXUK010000000
VERSION     DAZXUK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10027
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.139"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACH3L8_002682"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACH3L8_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFT4085738.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="ACH3L8_002683"
     CDS             complement(1433..1759)
                     /locus_tag="ACH3L8_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFT4085739.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="ACH3L8_002684"
     CDS             1894..2235
                     /locus_tag="ACH3L8_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFT4085740.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="ACH3L8_002685"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="ACH3L8_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="ACH3L8_002686"
     CDS             complement(2833..3543)
                     /locus_tag="ACH3L8_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFT4085741.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="ACH3L8_002687"
     CDS             3651..3956
                     /locus_tag="ACH3L8_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFT4085742.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..5568
                     /locus_tag="ACH3L8_002688"
                     /pseudo
     CDS             4155..5568
                     /locus_tag="ACH3L8_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(6295..7284)
                     /locus_tag="ACH3L8_002689"
     CDS             complement(6295..7284)
                     /locus_tag="ACH3L8_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085743.1"
                     /translation="MTMIKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGERPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTVKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7361..7852)
                     /locus_tag="ACH3L8_002690"
     CDS             complement(7361..7852)
                     /locus_tag="ACH3L8_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085744.1"
                     /translation="MLFNNNDWKLSVTDINLYENTVSLDGQSYPLSFAIKTLIPGYLS
                     GLPATSRESMELLEALAEAGVAIGNFFSNDLMTAYQRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQRELQRREQVKAERRTYGENLHSATHSAGRSRAAIVADIESGGNWM
                     DSL"
     gene            complement(7965..8168)
                     /locus_tag="ACH3L8_002691"
     CDS             complement(7965..8168)
                     /locus_tag="ACH3L8_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085745.1"
                     /translation="MAMIDPRTPIGKATLRYRGLPTRHLLSLLRLGVEDPERPFYSRD
                     ELIAMLVDRDLNNQLRRAFAKQS"
     gene            complement(8171..8668)
                     /locus_tag="ACH3L8_002692"
     CDS             complement(8171..8668)
                     /locus_tag="ACH3L8_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085746.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDTE
                     STQYKRFAQTKVGKLLRNRQKIRLIRMGELGSTVLADPAATVEFGRWLDFEYGLAVSE
                     AFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTAGR
                     CEWGK"
     gene            complement(8658..9173)
                     /locus_tag="ACH3L8_002693"
     CDS             complement(8658..9173)
                     /locus_tag="ACH3L8_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085747.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDPEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDTERLQAKADQEQRFKSFA
                     EALAGGASYDF"
BASE COUNT         2560 a         2535 c         2507 g         2425 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaaccctg cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccggat cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaacttcaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggacccccg gtggcctcgg
     5701 tctggacttg aggtgggcat atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagtc caaacctaag gacctagaag
     5821 tccacccaca gccttagaca gagtgtcgaa cacatccaca ccgagtcgac cagagaggac
     5881 catgtagaga cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtctgcggaa gtcatggaca taaagcctcc ttcgtctatt gtactactgc actagataac
     6001 tagtacaaaa caaaggtcca gaccattggt ccagaccata gttagttaat cacagtgata
     6061 gcccaccacc tgaagtcctg ctgatattca gtccagactt acctctgctc atggtcttac
     6121 ctccctcggt ctcctgacct tgggtatcga ctacagcttg ctgagcgtca tctggaggag
     6181 ctacaggagg agctacagga ggcgggaccg aagccccacc ttgagtccca ccaccacctg
     6241 tcaggccctt ggcgatggac ctgaagcctt tactaatgct cttacccatt ggtcttacgc
     6301 ttcggtcttg gtgaacttca ggcgtttgac tacaggcagg acggcagtag ctgagccagt
     6361 gcccatcttg acggtcgtag cagcttcagc gacaacgaac actttaccga ggatttccaa
     6421 gtcgtgggac tgagagtcgc catcacgtga aaggacgacc gtaatattct caccgtcgtg
     6481 acggaagcag ccgaagctca gggccgtgaa gcgtttagcc agcatgaaca cacctttgtc
     6541 ctcaccgttg taatacgcga tgtcggtacc cagcatatcc acgatagcgt cttcaccaga
     6601 gaagccctgt ttagcagcca gacgggacag cgctttcagt tgggactcat gggcgatgat
     6661 gccgtaatca gacacttcag agccgaaaga ggactcggtg ttctcaaaca gagcgtccat
     6721 gatagcgtct gccttagtgg tcaggcttcc agtcaggact ggcagctcta cggtcttcag
     6781 cagcttggag gactccagaa cgtcaaccat gcgagcgttg atggtacgga ccacattctc
     6841 acagatgttc atagcgtcct gctggagtgt gccatgagtg aaaccagtgg aggacttggc
     6901 gtcccacagt ttagtttgct ccgagatgag gattgcctgt tcgataggtt cgacgacaat
     6961 ctcctgacca gacggaacgg tcacgacttc aggtcgctca ccaccgcgta cagggacgac
     7021 cacagagcgg tcagacagag tgtgggcgat gattggggta ggcaggctat tgcgggtagg
     7081 caggacttct acagggaacg gaatgttatc ctgagcgtct ttaagtacac cgttcagcat
     7141 agccttaacc gtcttagcta actggagggc atagacctgt gggtcagcgt tagttggcag
     7201 accttcgtta acctgaatgt tgtcggtgtc ggtgatacgt actggctcaa tgccgaactg
     7261 atagctatct ttaatcatgg tcataataat caatctccta aattagtttt agatactgtg
     7321 ggtcccgaca tcaggtcagg accacttgtg tgtctgtgtc ttagaggctg tccatccagt
     7381 taccgcctga ctcaatgtca gcgacaatag cggcacggga gcgaccagcg gaatgggtag
     7441 cactgtggag gttctcacca taggtccggc gctcagcttt gacctgttcg cggcgctgta
     7501 gctcccgctg ccactctgcg tcggtcatgt tctcttcacg catacgctcg gcctgaagtc
     7561 gttgctcttt agcgatacgt tcagcctcag ctcgtttgtt catctggcgg cgttggtagg
     7621 cggtcatcag gtcattactg aagaagttgc caatagccac accagcttca gccagagctt
     7681 ccagtaattc catggactcg cggctggtgg caggcagtcc tgacaggtag cccggaatca
     7741 gggtcttaat agcgaaggac agtgggtagg actgaccgtc cagactcaca gtgttttcgt
     7801 agagattgat gtcggtgact gacagtttcc agtcgttgtt gttgaataac ataaattggt
     7861 actcctcttg agagttgaga ctatcggtat cgcagacgca gtagcgccta cgggtctccc
     7921 cgcatcggtc atctcaaaag gtgtttaaat taggtatggt atggtcagga ctgcttagca
     7981 aacgctcggc gtaactgatt attcaaatca cggtccacca gcatggctat cagctcgtct
     8041 cgactgtaga aaggacgctc ggggtcttcg actccaagtc tcagcagaga caggaggtga
     8101 cgtgtaggca gaccacggta acgaagggtc gctttgccga taggtgtgcg agggtcaatc
     8161 atagccatga ttacttaccc cactcacagc gaccagcggt cacagcttga tagtctgaat
     8221 actcagcctg tgagacttca cgtccggcga tgtagaggcc tgtgccatga cgctggatga
     8281 attccttggt ctccggcttg atgtcctcag cagacgctac agcagactgc acggtggcgc
     8341 tggaggtcat agacacgaag gcttcagaga ctgccagtcc gtactcaaag tccagccagc
     8401 gaccaaactc tacagtagcg gcggggtcag caaggacggt agacccgagt tcacccattc
     8461 ggataaggcg gattttctgc ctattcctca gtagctttcc aactttggtc tgagcaaaac
     8521 gcttgtactg tgtcgactcg gtgtcttcta ctttgccaat ctgaatgccg acccggcgaa
     8581 tttcatcgag tcgaaacaat ccgtcctgac tggtgatgtc ctgaccgaag atatgaatgg
     8641 tgtattcctg cttattgtca gaagtcataa gaggcacctc ctgccagagc ttcggcaaag
     8701 gacttgaagc gctgctcttg gtcagctttc gcctgaagac gttcggtatc aggtgggacc
     8761 agcttatcaa aggtgccgtt ttcgtaatcc tcagttagga cctgaagtcg gcgggaccat
     8821 ttgctctcca cctgcatcat cgcgttagag atgtgcttca agtcggcatc agagcgtgac
     8881 gctatccaat ccatctcttc tgggtcaccc tcacaggtgt aaggattgtt gaaatagaag
     8941 atgtcctgta ggccacgttt cagctcttcc tcggtgtgtg tctggaggta tgactggata
     9001 ggctccgcgt acatctcccg gattttctca tagtcccagt cttccgcttg ggcataccat
     9061 cgggataact tcagcaggtc taagttatag cgacagcaat cgacactgga ctctgcattg
     9121 gtcagcagga tgattaagaa ctggcggtta gttactggta attcggtatt catataaagg
     9181 atgctccttt ggtaaagcac tttctgtctc cacgtggagt ccgcacctat tcaatgcatc
     9241 caaacggacc caccaaaaag tgcttaacga aagtgaatag gattaaggat ggtctggagt
     9301 gacccggtgt ctctacatgt acgtataaag gcgagaccgc atgtcaggac gagctgtaag
     9361 gaccccagat ggtgagcggt gatagactta agccaatgta gtgagtagac gagctactaa
     9421 ctgagatgac tgaagaacgc taacgagctg gaggtcatac aggaggtctg acagaggcgg
     9481 tcacagacct tgactactta ggagagatac ttaatgtcgg ttgaccagat gctcgcatgt
     9541 tctgacgcat cagactcaga ggcaaggttg gtctccctat agtgatagtt taggggggtg
     9601 tccctatagt gagggggtat tccagcccag agctggcgcg gctctcagga caggcactta
     9661 tcgttgtagt cccaccatgt gaaatcccct cggtccttca gaaccttagc aagactcaca
     9721 gccgtagcct gcaagacgct attaacgtgg cgcaggtagc gaggcgaagg tgagcgaccc
     9781 gtgatggcgg tgtatgactt ggcagtgttg cgggagaaga tgtcgtttgt gccgtataag
     9841 gccgcgaaca ggtacgcctt ggataccggg agtccagcac cgatagagcc actggtagcc
     9901 cgtgagagtc ctgtgaacac atcgtcagcc cacgggtacg cctcaacaag ctgcgtatag
     9961 gagagacacc cagtctcaat cagccacgca cggacagccc ggttgtcgat gaccacagga
    10021 ggcttac
//
LOCUS       DAZXUK010000140         9918 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZXUK010000140 DAZXUK010000000
VERSION     DAZXUK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9918
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.140"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            327..1322
                     /gene="pgl"
                     /locus_tag="ACH3L8_002694"
     CDS             327..1322
                     /gene="pgl"
                     /locus_tag="ACH3L8_002694"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFT4085748.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1363..2316)
                     /locus_tag="ACH3L8_002695"
                     /pseudo
     CDS             complement(1363..2316)
                     /locus_tag="ACH3L8_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2500..3552
                     /locus_tag="ACH3L8_002696"
     CDS             2500..3552
                     /locus_tag="ACH3L8_002696"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFT4085749.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3628..5061
                     /locus_tag="ACH3L8_002697"
     CDS             3628..5061
                     /locus_tag="ACH3L8_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFT4085750.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5244..7424
                     /locus_tag="ACH3L8_002698"
     CDS             5244..7424
                     /locus_tag="ACH3L8_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFT4085751.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7565..8848)
                     /locus_tag="ACH3L8_002699"
     CDS             complement(7565..8848)
                     /locus_tag="ACH3L8_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFT4085752.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(8983..>9918)
                     /locus_tag="ACH3L8_002700"
     CDS             complement(8983..>9918)
                     /locus_tag="ACH3L8_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4085753.1"
                     /translation="TEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILAS
                     RGIKQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTL
                     SDAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMEL
                     AVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCK
                     EILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQ
                     ISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2399 a         2318 c         2519 g         2682 t
ORIGIN      
        1 tatttgtgcc ggaagtgagt tgattgttgc gcgaatggga agttggccag aagttgttat
       61 ggcatagtgt ttacggtcgt tgatacaggt aaacaaaagc ggggagagtc atactccctg
      121 aatagtcgcg aaaatatctg tggcattgtc aaattcgtgt cggtgactaa tattttagta
      181 atatgccatt cgaaatggag tgctaactta ttgatttaat tgatgtgcga tattgtattt
      241 tatcgtgttt tgttgtctgt tttttatcca gtaagtgttg ttatatgtat gaaagataag
      301 ggatttatca tttttgaggt gtgttcatga agcaaacagt ttatatcgcc agccctgaga
      361 gccagcaaat tcacgtctgg aacctgaatc atgaaggcgc actgacgctg acacaggttg
      421 tcgatgtgcc ggggcaggtg cagccgatgg tggtcagccc ggacaaacgt tatctctatg
      481 ttggtgttcg ccctgagttt cgcgtcctgg cgtatcgtat tgccccggac gatggcgcac
      541 tgacctttgc cgcagagtct gtgctgccgg gtagtccgac gcatatttcc accgatcacc
      601 aggggcagtt tgtctttgta ggttcttaca atgcgggtaa cgtgagcgta acgcgtctgg
      661 aagatggcct gccagtgggc gtcgtcgatg tagtcgaggg gctggacggt tgccattccg
      721 ccaatatctc accggacaac cgtacgctgt gggttccggc attaaagcag gatcgcattt
      781 gcctgtttac ggtcagcgat gatggtcatc tggtggcgca ggaccctgcg gaagtgacta
      841 ccgttgaagg ggccggcccg cgtcatatgg tattccatcc aaacgaacaa tatgcgtatt
      901 gcgtcaatga gttaaacagc tcagtggatg tctgggaact gaaagatccg cacggcaata
      961 tcgaatgtgt ccagacgctg gatatgatgc cggaaaactt ctccgacacc cgttgggcgg
     1021 ctgatattca tatcaccccg gatggtcgcc atttatacgc ctgcgaccgt accgccagtc
     1081 tgattaccgt tttcagcgtt tcggaagatg gcagcgtgtt gagtaaagaa ggcttccagc
     1141 caacggaaac ccagccgcgc ggcttcaatg ttgattacag cggcaagtat ctgattgccg
     1201 ctgggcaaaa atctcaccac atctcggtat acgaaattgt tggcgagcag gggctactgc
     1261 atgaaaaagg ccgctatgcg gtcgggcagg gaccaatgtg ggtggtggtt aacgcacact
     1321 aaccgctgat ttaaccggcg cagtctctcc tgcgccggtg tattaactta tctcctgtaa
     1381 cgcatgtctc tggcgttcga cgatattggt ccacaaattg tctttatcgt cagtccacaa
     1441 attaatcagc aaggaaaaaa agcgttcagc tgccggagaa agcacggcat ctttgcggcg
     1501 aataatcccc aatgtccgac gtataacggg ttccaccagt gggataccaa taatcgaaga
     1561 atagggcgca tggggcattg ccagaccagg aagcgccgaa atccccagtc ctgcctccac
     1621 tagtcctaat gacgtcgaaa gatgacgcac ttcgtaaaac cagtccagct tccagggctt
     1681 gtcggctagc tgttgttcta tcaacaatcg gttgccgctg gaggaacgta cgccaatcat
     1741 tttgtaaccc actaactcct gccattctac aagctacttt ttggccaatg gatggtcacg
     1801 ccgacaggcc agcacgaatg gctcgttgac cagtggggta aaatcaatgg atgaatttgt
     1861 gacgttgttc atgtttatgc caaagtctga ttcgttacac agcaccgatt ccatgcaatt
     1921 attcgtgcct tgttccagaa tccgcacttt aatattggga tacagctcat taaatttacc
     1981 gattgccagc ggtaaaaaat aaaatactgc ggtcggaata cacgctaatg tcaccatacc
     2041 acgatgataa gcattcatat cacgaatatt aaaaagcgtt tcatcgaatt tctttattaa
     2101 ttcccgcgcc tccgggagca acctttttcc tgcttttgtt aacgtaacct tgcgagttgt
     2161 acgctcaaat agttgaatat gtaaatcttc ctccatcttt tttattcttc gcgttaatgc
     2221 aggttgcgta atattgagta atttagcggc attgttaaat gaactggact ctgccagtat
     2281 gacaaatgcc ttcatacttg ataattcatg cttcattatt actccggaaa atggaagcga
     2341 cgattttggg gggctggtcg ttaaaaattt taactgcatt tagccaactt aaattaatga
     2401 aaaaatgtta ttaatcgttg agctaaagtc attagagatg ctttgtcctt aatgtaacca
     2461 tatcgcaata agttatgttt ttaaattgag ggcattatta tgaaaaaaat accctgcgtg
     2521 atgatgcgag gtggaacctc gaggggcgcg ttcctgttag cggaacattt acccgaagat
     2581 caaacgcagc gcgataaaat attgatggca attatgggtt ccggtaacga tctggaaatt
     2641 gacggtattg gcggcggtaa tccactgacc agtaaagtcg ccattattag ccgttccagc
     2701 gatccgcgtg ctgatgtcga ttatctgttt gctcaagtta tcgtccatga gcaacgtgtc
     2761 gataccacgc ctaactgcgg caatatgctg tctggcgtag gggcattcgc cattgaaaat
     2821 ggtttgattg cagcgacttc gccagttacc cgtgtacgta tccgcaacgt caatacgggt
     2881 acgttcatcg aagctgatgt gcaaacgcca aatggtgttg tcgagtacga gggtagcgcc
     2941 agaattgacg gcgtaccggg tactgccgca ccggttgcgc tcactttcct gaatgccgct
     3001 ggaacgaaaa ctggaaaagt tttcccgact gataatcaga ttgattattt tgacgatgtc
     3061 ccggtgacct gtatcgatat ggcgatgcca gtcgtcatta ttccggctga atatctggga
     3121 aaaacaggtt atgaattacc ggcggaactg gatgccgaca aagcattatt agcccgcatt
     3181 gaatctatcc ggctacaagc gggtaaagca atgggcttag gtgatgtcag taatatggtt
     3241 atccctaaac ctgtgcttat ttctccagcg cagaaaggcg gggcaattaa tgtgcgttat
     3301 tttatgccgc attcttgcca tcgcgcgctg gcgataaccg gtgctattgc tatttccagt
     3361 agttgtgcat tggaaggcac cgtcacccga caaatcgtcc cttctgtagg atatggcaat
     3421 atcaatattg aacaccccag tggtgcgctc gacgttcatt taagtaatga aggtcaggat
     3481 gccacgacgt tacgcgcatc tgttattcgg acaaccagaa aaatattttc cggtgaagtt
     3541 tatcttccct gaaaaaattc gttgtcagga taaggacaat caataaagga cttctgtatg
     3601 agtcatacag aaagaacagg attttaaatg aataagaaat cgttatggaa gctaattctg
     3661 atattagcga tcccatgtat tattggtttt atgccagctc cggcaggatt aagcgaactg
     3721 gcgtgggtgc tttttggtat ttacctggcg gccattgtgg gtctggttat caagcctttc
     3781 ccggaacctg tcgtactgtt aattgccgtt gctgcctcaa tggtggtggt cggtaactta
     3841 tccgacggtg cgtttaagac caccgccgta ttaagcggtt actcttcagg tactacctgg
     3901 ctggtgttct cggcgtttac cttaagcgcc gcatttgtga ccaccggttt aggtaaactt
     3961 attgcctatc tgctgattgg taaaatcggt aacaccatgc tgggtctggg ttacgttacg
     4021 gtattcctcg atctggtact ggctccggca acaccgtcta acaccgcgcg tgcgggcggc
     4081 attgtgttac cgatcatcaa cagcgtggcg gtggctttgg ggtccgaacc ggaaaaaagt
     4141 ccgcgtcgtg tcggacatta cctgatgatg tccatttaca tggtcaccaa aaccaccagc
     4201 tatatgttct ttaccgcaat ggcggggaac attctggcgc tgaaaatgat caacgacatt
     4261 ctgcacctgc aaattagctg gggtggatgg gcgctggccg ccggattgcc gggcatcatt
     4321 atgctgctgg tcaccccgct ggtaatttac accatgtatc caccagaaat taaaaaggtg
     4381 gataacaaaa ccatcgctaa agcgggcctt gccgaactgg gaccgatgaa aatccgcgaa
     4441 aaaatgctgc tcggtgtctt tgtgctggcg ctactgggct ggattttcag taagtctctg
     4501 ggggttgatg aatccactgt ggcaatcgtt gttatggcga ccatgctgct gctgggtatc
     4561 gttacctggg aagacgtggt taaaaataaa ggcggctgga ataccttaat ctggtacggc
     4621 ggtattatcg gcttaagctc cttattatcg aaagttaaat ttttcgaatg gttagctgaa
     4681 gtctttaaaa ataacctggc atttgatggt cacggtaacg ttgctttctt cgttattatt
     4741 ttcctcagca tcatcgtgcg ttatttcttc gcttccggta gtgcctatat cgttgctatg
     4801 ttacctgtat ttgccatgct ggcgaacgtc tccggcgcac cgttaatgtt aaccgcgctg
     4861 gcactgttgt tctccaactc ctatggcggc atggttactc actatggcgg cgcggcaggt
     4921 ccggtcatct ttggcgtggg ttacaacgat attaaatcct ggtggttggt cggtgcggta
     4981 ctgacgatat taaccttctt ggtgcatatc accctcggcg tgtggtggtg gaatatgctg
     5041 atcggctgga acatgctgta aatatactcg tcatacttca agttgcatgt gctgcgtctg
     5101 cgttcgctca ccccagtcac ttacttatgt aagctcctgg ggattcactc acttgtcgcc
     5161 ttcctgcaac tcgaattatt tagagtatat ccatttatta tctttctgcg cacttcacgg
     5221 tgcgcagata tctggagcat ttgatgatca agttatctga aaaaggcgtg tttctcgcca
     5281 gtaataacga aataattgcc gaagaacatt tcaccggcga aattaaaaaa gaagaagcaa
     5341 aaaaaggcac tattgcctgg tctattctct cttctcataa tacgtccgga aatatggata
     5401 aacttaaaat taagtttgat tcattagcct ctcacgatat tacctttgtt ggtattgtac
     5461 agaccgctaa agcgtccgga atggaacgtt tcccgctgcc gtatgtgctg accaactgcc
     5521 ataactcact ctgcgccgtc ggcggcacca ttaacggtga tgaccatgtt tttggtttat
     5581 cggcagctca gcgttatggc ggtatttttg tgcctccgca tattgcggtc atccatcaat
     5641 atatgcgtga gatgatggct ggcgggggca aaatgatcct cgggtcagac agccacaccc
     5701 gttacggtgc attagggaca atggcagtcg gtgagggtgg cggtgagttg gtaaaacagc
     5761 tgcttaatga cacctgggat atcgactatc cgggcgtggt tgcggtgcat ctgaccggaa
     5821 aacctgcgcc gtatgtgggg ccacaggatg tggcgctggc tatcattggc gcggtgttca
     5881 aaaacggtta cgtcaaaaac aaagtcatgg agttcgttgg accgggcgtt agcgcgctct
     5941 ctaccgattt ccgtaacagc gttgacgtga tgaccacgga aaccacctgt ttaagctccg
     6001 tctggcaaac tgatgaagaa gtgcataact ggctggcgct gcacggtcgc ggtcaggatt
     6061 actgccagct taaccctcaa ccgatggcgt actacgatgg ctgcatcagc gttgatttaa
     6121 gcgccatcaa accaatgatt gcgctgccgt tccacccgag caacgtgtat gaaatcgaca
     6181 cactgaacca gaacctgacc gacattctgc gtgagattga aattgagtcc gaacgcgtgg
     6241 cgcacggtaa agccaaactc tcgctgctgg ataaagtgga aaatggtcgc ctgaaagtgc
     6301 agcaggggat tatcgcgggc tgttctggcg gtaactacga aaacgtcatc gcggcggcga
     6361 atgcactgcg cggtcaatcc tgtggcaatg acaccttctc gctggcggtt tacccgtcat
     6421 cacagccggt gtttatggat ctcgccaaaa aaggtgtggt agcagatttg attggcgcag
     6481 gcgcaatcat cagaaccgca ttctgtggcc catgctttgg cgcgggcgat acgccaatca
     6541 acaacggttt gagtattcgc cacaccacgc gtaacttccc gaaccgcgaa ggctctaagc
     6601 cagccaatgg gcagatgtca gcggtggcgt tgatggacgc tcgttctatc gctgcgactg
     6661 cggcaaacgg tggctattta acctctgcca gcgaacttga ttgctgggac aacgtgccgg
     6721 agtacgcctt cgatgtaacg ccgtataaaa accgtgttta tcagggcttt gtgaaagggg
     6781 caactcagca accgctgatt tacggaccga acattaaaga ctggccggaa ttgggtgcgc
     6841 tgactgacaa tatcgtcctg aaagtgtgct cgaagatcct cgacgaagtc accaccaccg
     6901 acgaactgat tccttccggt gaaacctctt cttatcgttc aaatccgatt ggtctggcgg
     6961 ggaatgtcag cgagctgaca gaggtgtttg cgcgcattaa gcagattgct ggtcaggagc
     7021 atattgatcc gctgcaaact gaaattggca gcatggtcta tgcggtgaaa ccaggcgatg
     7081 gttctgcgcg tgaacaggcg gcgagctgcc agcgtgtgat tggcggtctg gcgaatattg
     7141 ccgaggagta cgcgactaaa cgctaccgtt ctaacgtcat caactggggg atgttaccgc
     7201 tgcagatggc ggaagtgcca acctttgaag tgggggatta catttacatc cctggcatta
     7261 aagcggcgct ggataatccg ggtacgacgt ttaaaggtta tgtgatccat gaagatgcgc
     7321 cggtaacgga aattacgctt tacatggaaa gcctgacggc tgaagagcgc gagattatca
     7381 aggcgggtag tttgattaac ttcaataaaa accgtcagat gtaaaaagcg ccatgtgaat
     7441 gtaggtcgca ttcggcactt attgtcggat gcgatgctgg cgcatcttat ccgacctaca
     7501 aatcgcatcg aaccgtaggc cggataaggc gtttacgccg catccggcaa atagttaata
     7561 gctcttactt cttctcctct gcaaccactt tgctgcccac gccgcggtta ttgtattccc
     7621 acatgcggtt gtagttagtg tcattcagat tgcgctgtat ttcgtcgtta tcatctacgc
     7681 tgccggtatt acccgcaaac ggacgattag agatcaccgc atcggcccac ggtttagccg
     7741 tgttaaaacc ttcgttgatg gcgctatcac ggatcaccac ctgaccgttg gtattagcat
     7801 caacatccag cgagcggccc agttgcgcca cgccatcacc ggaagcattg aaacggctgt
     7861 ttacggcgag gaaaccgtaa tagatgttag acagcgtagc cggtgcaaac acatatgctt
     7921 cttgctgggt acgtgagttc accacgcgga attcggtgtt atcgaacacc actgcgccgc
     7981 gaccagaaac gatatccaca tccccttcaa tatagctgtt ggtcaccagc gtacgcggct
     8041 gacggttggt ttccagacgg ttctgcacac cgctgttggt gacaaagaag gtgttctgac
     8101 gaccgagaat gttaacgttg ttaatctgta cttggtcacc gtcagtacgc agtgctaccg
     8161 ccggatggtt acctgcatct acgctatcgc ccagcgtgtt ttcgatggtc agattttgca
     8221 gttgcagtcc attgttttgt gaccagaaga ccgcagaaca cagaacaccg atactgtcgc
     8281 tgcgtttgct ctggcagcta tcgtacatat accacgctgg tttacctggc atatatttgc
     8341 cgcgcgggtt gacgtcgtga cgccagtcgg cagggctcat gcccccatca agggaaagcc
     8401 caatcttcac atcaatcggt ttttcacctg taccgtacag agtgattcca cctggagcag
     8461 cagggacata caccgttccc tgatactcac caggcatcac ggcaatatac tggcgcttgt
     8521 tggtgcgctt gataattgcc gcatctaccg ccgcctgaat cgtggtatgc gttacacctt
     8581 gagtacccgc cgggccgaca acaaagtcag gttgcgcagg cagggttatc ggggacggat
     8641 tccacgctgc agcacctggt gtcagggatg caaaatagtg ttgcgcatcg aaattcagcg
     8701 cttcttttgc cgacagaatc gggcgagaag aggtaccagg cgcagtttga tcagaaggac
     8761 gttgatcggg tggtgttgag ctacaggcgg tcagcgtcac gccaaaagcc aatgccagcg
     8821 ccagacggga aactgaaaat gtgttcacag gttgctccgg gctatgaaat agaaaaatga
     8881 atccgttgaa gcctgctttt ttatactaag ttggcattat aaaaaagcat tgcttatcaa
     8941 tttgttgcaa cgaacaggtc attatcagtc aaaataaaat cattatttga tttcaatttt
     9001 gtcccactcc ctgcctctgt catcacgata ctgtgatgcc atggtgtccg acttgtgccc
     9061 gagaagatgt tgagcaaact tatcgcttat ctgcttctca tagagtcttg cagacaaact
     9121 gcgcaactcg tgaaaggtag gtggatcccc ttcgaaggaa agacctgatg cttttcgtgc
     9181 gcgcataaaa taccttgata ctgtgccgga tgaaagcggt tcacgacgag tagatgcaat
     9241 tatggtttct ccgccaagaa tctctttgca tttatcaagt gtttccttca ttgatatccc
     9301 gagagcatca acatgcaatg ttgtagggat ggcaattttt acgcctgttt tgctttgctc
     9361 gacataaaga tatccatcta cgatatcaga ccacttcatt tcgcataaat caccaactcg
     9421 ttgcccggta acaacagcca gttccattgc aagtctgagc caacatggtg atgattctgc
     9481 tgcttgataa attttcaggt attcgtcagc cgtaagtctt gatctcctta cctctgattt
     9541 tgctgcgcga gtggcagcga ccgggtttgt tgttatatgg ccttcagcta ttgcctctcg
     9601 gaatgcatcg ctcagtgttg atctgattaa cttggctgat gccgccttgc cctcgtctat
     9661 gtatccattg agcattgccg caatttcttt tgtggtgatg tcttcaagtg gagcatcagg
     9721 cagccccctc cttattgctt taattttgct catgtaattt atgagtgtct tctgcttgat
     9781 tcctctgctg gcgaggattt tttcgtagcg atcaagccat gaatgtaacg taacagaatt
     9841 atcactgttg attctcgctg tcagaggctt gtgtttgtgt cctgaaaata actcaatgtt
     9901 ggcctgtata gcttcagt
//
LOCUS       DAZXUK010000141         9885 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZXUK010000141 DAZXUK010000000
VERSION     DAZXUK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9885
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.141"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            429..1547
                     /locus_tag="ACH3L8_002701"
     CDS             429..1547
                     /locus_tag="ACH3L8_002701"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFT4085754.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FMALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1613..1861
                     /locus_tag="ACH3L8_002702"
     CDS             1613..1861
                     /locus_tag="ACH3L8_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFT4085755.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1926..2294
                     /locus_tag="ACH3L8_002703"
     CDS             1926..2294
                     /locus_tag="ACH3L8_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFT4085756.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2388..3041
                     /gene="nfsB"
                     /locus_tag="ACH3L8_002704"
     CDS             2388..3041
                     /gene="nfsB"
                     /locus_tag="ACH3L8_002704"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFT4085757.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3149..4396
                     /gene="ybdG"
                     /locus_tag="ACH3L8_002705"
     CDS             3149..4396
                     /gene="ybdG"
                     /locus_tag="ACH3L8_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFT4085758.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="ACH3L8_002706"
     CDS             complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="ACH3L8_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFT4085759.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="ACH3L8_002707"
     CDS             complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="ACH3L8_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFT4085760.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9096..9614)
                     /gene="cusB"
                     /locus_tag="ACH3L8_002708"
                     /pseudo
     CDS             complement(9096..9614)
                     /gene="cusB"
                     /locus_tag="ACH3L8_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2423 a         2733 c         2394 g         2335 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagggttg atagaaatat
       61 ctcctggggc ttcatctttc cagacctcag agtaaacctg aaatcggaaa gcttcaggca
      121 cccgcagtta tcttaccctt cctcaacaaa atagctgttt atttatacag caaatcctgt
      181 ttcggacttc tcactcccca tttgatcaac tgaagctgaa cccgtttttt tctgcatgcc
      241 tcaatcgtca aaattcttcg ccaccagcca gctctgccat aacttgccac attgaaaaag
      301 tagccaaagt gcttcaggca ccatcagttc accatcacca aaaaaaagaa acattccaga
      361 acttccgcat ctcgcccagc cgctccgact tctatactga atagaaaacg tcaacttaag
      421 agaaacctat gccattaccc gattttcatg tttctgaacc ttttaccctc ggtattgaac
      481 tggaaatgca ggtggttaat ccgccgggct atgacttaag ccaggactct tcaatgctga
      541 ttgacgcggt taaaaataag atcacggccg gagaggtaaa gcacgatatc accgaaagta
      601 tgctggagct ggcgacggat gtttgccgtg atatcaacca ggctgccggg cagttttcag
      661 cgatgcagaa agtcgtattg caggcagccg cagaccatca tctggaaatt tgcggcggtg
      721 gcacgccccc gtttcagaaa tggcagcgtc aggaggtatg cgacaacgaa cgctatcaac
      781 gaacgctgga aaactttggc tatctcatcc agcaggcgac cgtttttggt cagcatgtcc
      841 atgttggctg tgccagtggc gatgacgcca tttatttgct gcacggcttg tcacggtttg
      901 tgccgcactt tatggccctt tccgccgcgt cgccatatat gcagggaacg gatacgcgtt
      961 ttgccccctc acgaccgaat attttttccg cctttcctga taatggcccg atgccgtggg
     1021 tcagtaactg gcaacaattt gaagccctgt ttcgctgtct gagttacacc acgatgatcg
     1081 acagcattaa agatctgcac tgggatattc gccccagtcc tcattttggc acggtggagg
     1141 ttcgggtgat ggataccccg ttaaccctta gccacgcggt aaatatggcg ggattaattc
     1201 aggccaccgc ccactggtta ctgacggaac gcccgttcaa acatcaggag aaagattacc
     1261 tgctgtataa attcaaccgt ttccaggcct gtcgctatgg tctggaaggc gtcattaccg
     1321 atccgcacac tggcgatcgt cgaccgctaa cggaagatac cttgcgattg ctggaaaaaa
     1381 tcgccccttc agcacataaa attggcgcat cgagcgcaat tgaagcactg catcgccagg
     1441 tcgtcagcgg tctgaatgaa gcgcagctga tgcgcgattt cgtcgccgat ggcggctcgc
     1501 tgattgggct ggtgaaaaag cattgtgaga tctgggccgg tgactaagcc gggaattgcc
     1561 ttacagaaga atatccctga caatggtgtt ttaacttaca tttaacaatg ttatgaaaca
     1621 ccctttagaa accttgacca ccgcagcagg cattttgctg atggctttcc tctcttgcct
     1681 gctgctgccc gcccccgcac tggggctgac gctggcacaa aaactggtga ccatgttcca
     1741 tctgatggat cttagccagc tttacacttt attgttttgt ctgtggtttt tagtgctggg
     1801 cgctattgag tattttgttc tgcgctttat ctggcgacgc tggttctcgc tggcggatta
     1861 aacgtcgatt atcgccgaac ttcgcggcat actttgctta ttctccttcg cctaaaggaa
     1921 cgtttatgga taagcaatca ctgcacgaaa cggcgaaacg cctagctctt gagttaccct
     1981 ttgtcgagct ttgctggcct tttggcccgg agttcgatgt ttttaaaatt ggcggcaaga
     2041 tttttatgct gtcgtcggag ctacgcggcg tcccctttat caatctgaag tccgatccac
     2101 aaaaatccct gttaaatcag caaatatacc caagcattaa gccagggtat cacatgaata
     2161 aaaagcactg gatttcggtg tattccggcg aggaaatctc cgaagcgtta cttcgcgatc
     2221 tgatcaacga ttcgtggaat ctggtggttg atggtctggc taaacgcgat caaaaaagag
     2281 tgcgtccagg ctaaagcggg aatctatagc gcatttttct cgcttaccat ttctcgttga
     2341 accttgtaat ctgctggcac gcaaaattac ttacacatgg agtctttatg gatatcattt
     2401 ctgtcgcctt aaagcgtcat tccactaagg catttgatgc cagcaaaaaa cttaccccgg
     2461 aacaggccga gcagatcaaa acgctactgc aatacagccc atccagcacc aactcccagc
     2521 cgtggcattt tattgttgcc agcacggaag aaggtaaagc gcgtgttgcc aaatccgctg
     2581 ccggtaatta tgtgttcaac aaacgtaaaa tacttgatgc ctcgcacgtc gtggtgttct
     2641 gtgcaaaaac cgcgatggac gatgcctggc tgaagctggt tgttgaccag gaagatgccg
     2701 atggccgctt tgccacgccg gaagcgaaag ccgcgaacga taaaggtcgc aagttcttcg
     2761 cctatatgca ccgtaaagat ctgcatgatg atgcagagtg gatggcaaaa caggtttatc
     2821 tcaacgtcgg taacttcctg ctcggcgtgg cggcgctggg tctggacgcg gtgcccatcg
     2881 aaggttttga cgccgcaatc ctcgatgcag aatttggtct gaaagagaaa ggctacacca
     2941 gtctggtggt tgttccggta ggtcatcaca gcgttgaaga ttttaacgct acgctgccga
     3001 aatctcgtct gccgcaaaac accaccttaa ccgaagtgta attctctctt gccgggcatc
     3061 tgcccggcta tttcccctca gattctcctg atttgcataa ccctgtttca gccgtcatca
     3121 taggctgctg ttgtataaag gagacgttat gcaggattta atatcccagg ttgaagattt
     3181 agcgggtatt gagatcgatc acaccacctc gatggtgatg attttcggta ttatttttct
     3241 gaccgccgtc gtggtgcata ttattttgca ttgggtggta ctgcggacct tcgaaaaacg
     3301 tgccatcgcc agttcacggc tttggttgca aatcattacc cagaataaac tcttccaccg
     3361 tttagctttt accctgcagg ggattatcgt caatattcag gcggtattct ggctgcaaaa
     3421 aggcaccgaa gcggcagata ttctgactac ctgcgcgcag ttgtggatca tgatgtatgc
     3481 gctgctttca gtcttctcgt tgctggatgt tattttgaat ctggcgcaga aattcccggc
     3541 ggcatctcag ttaccgctga aagggatatt tcaggggatt aaactgatcg gcgcaatcct
     3601 ggtcggcatt ttgatgatct cgctgctgat tggtcagtcg ccagcgattc taatcagcgg
     3661 tcttggtgca atggctgccg tgctgatgtt ggtatttaaa gatccgattc ttggtctggt
     3721 ggcaggtatt cagctttccg cgaacgatat gctgaaactg ggcgactggc tggaaatgcc
     3781 gaaatacggc gcggatggcg cggtgatcga tattgggtta accaccgtca aagtgcgtaa
     3841 ctgggacaat accattacca ctattcccac ctggtctctg gtttctgact cctttaaaaa
     3901 ctggagcggg atgtcagcat ctggcgggcg acgtattaag cgcagtatca gtattgatgt
     3961 caccagtatt cgttttcttg atgaagacga aatgcaacgt ctgaataaag cgcatttgtt
     4021 aaagccttat ttaaccagcc gccatcagga aattaatgag tggaatcgcc agcaaggttc
     4081 tacggagtcg gtattaaacc tgcgccgaat gaccaatatt ggaacctttc gtgcctatct
     4141 gaacgaatat ctgcgtaacc atccgcggat tcgtaaagat atgaccttaa tggtacgcca
     4201 actggctcca ggtgataacg gtttaccgct cgagatctac gcgtttacta acaccgtggt
     4261 gtggctggaa tatgaaagca ttcaggctga tatattcgat cacatatttg cgattgtcga
     4321 agagtttggt ctgcgacttc atcagtcgcc aaccggcaat gatattcgct ctctggcggg
     4381 tgcatttaag cagtaattaa aaaaaccgct ctcatcgaat ggatgagagc ggacatcgtt
     4441 ttgtcggatg tagcgtgaat gccttatttc cgacgcagcg ttttaaatgc cacaaacagg
     4501 aatacaatcc acaccggcag caggatcgct gacaagcgca tatcatccat cgtgcacatc
     4561 agcagcaaaa tcatgccgag gaaggcgatg caaagatagt tgccgaacgg atagagcagc
     4621 gccttaaact gtgtttcacg cccctgacgt cgcatcgccg cgcgaaaacg cagatgcgcc
     4681 aggcagatca taatccagtt caacagcaac gttgctacca ccagcgccat cagcagacca
     4741 aacgcttttt gcggcagcag atagttgatt aacaccacca gcgaagtgat cgctccggaa
     4801 agcatcagcg agttaatcgg cacaccgcga cggctgacgc gagtcaaaaa cttcggcgca
     4861 ttaccctgca cagaaaggcc aaacagcatg cggctgttag agtaaacccc gctgttatac
     4921 accgacagcg atgccactag aatgacgaag ttcagcgcag aagctaccac attgctgtcg
     4981 agattatgga aaatcatcac aaacgggcta ctgttggatt tcacttccac ccacggatag
     5041 agtgccagta aaaccaccag tgaaccgata taaaacagca ggatgcgata caccacctga
     5101 tttaccgctt ttggaatgct tttttccgga tcgcgcgctt cagcggctgt aatcccaatc
     5161 agctccagac cgccgaagga gaacataatt accgccagcg acaaaatcag cccattccag
     5221 ccggtggcga agaaaccacc gtagcgccag aggttgtcga tactggcttt ctcgccgccg
     5281 taaccagaaa acagcagcca caggccaaag ccgatcatac cgatgatcgc cagcacttta
     5341 atcagcgcaa accagaactc ggtttcgcca tataagcgca cgttcaccag gttaacggcg
     5401 ttgatgataa taaagaaggc ggcagcccaa atccacgttg gaacatccgg gaaccagtac
     5461 tgcatataga tgcccgcagc ggtcagctct gccattccca ccagcacgaa cattacccag
     5521 tagttccagc cagagaggaa gcccgcaaac ggtccccagt atttataggc aaagtgggca
     5581 aatgaaccgg ataccggctc ttcgaccacc atctcgccaa gctggcgcat aatcaggaaa
     5641 gcgatgatcc cggcgacgcc gtagcccagc aatacagccg gacccgccat ctgaatcgcc
     5701 gggccaatgc caagaaacag accagtacca attgcgccac ccagcgcaat cagttgaata
     5761 tgacggttat gtaatccgcg atgaagcgtc ggctcttgat tcgacgcagt atcttccgat
     5821 acggttgacg cgtttttcac gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt
     5881 taacatttag tgcccatcgt cgcaagacac aatccacacg gttaaacgag gtatcctgct
     5941 ttaattccgt acccgatgtc ggtgcagcca catcagctta tacgccgccg ggataataaa
     6001 cagcgacagc aaaggtgcgg tgatcatgcc gccaatcatc ggtgcggcaa tccggctcat
     6061 cacctctgaa ccagcccccg ttccccacag aatcggcagc agaccggcga taatcaccgc
     6121 caccgtcatc gctttcgggc gcacgcgcag gaccgcgccg tgatacaacg cctcatccag
     6181 cttctgctcg ctgaatgttt gtggattatt caacgacggc tcggcctcta tggcgtgacg
     6241 taaatacatc agcatcacca cgccaaattc ggcggcgacc ccggcaaggg caataaagcc
     6301 ggtacccgtc gccacagaaa gatgaaagcc catccaccac agtaaccaga tgccgcccac
     6361 cagcgcaaac ggtacgctgc tgataatcag caacgcttcg cccacccgac ggaatgccag
     6421 atacaacagc acgaagatga tcatcagtgt catcggcacc atgagtttca gcttatggtt
     6481 ggcgcgctcc agtagctcga actgcccgga gaatgccacg ctggtgcccg gttttaactg
     6541 cactttttca gctatcgctt tttgcaaatc gtgaaccacc gacaccatgt cacgatcgcg
     6601 ggcatcgata taaatccagc tcgtcgggcg cgcattctcg gttttcagca tcgacggtcc
     6661 ggtagagact ttaacgtcgg ccacgtctgc cagggtgatt tgctgcttca tcggcgtcag
     6721 gatcggcaac tggcgcagtg cctgcggact atcgcgccag ctttgcggat aacgcagatt
     6781 aattggataa cgggcaatcc cttccaccgt ttcgccaacc atcgccccgc ccaccgcaga
     6841 agtcacaaac aactgcacat ccgccaccgt cataccgtaa cgtgcggctt tttcacggtt
     6901 aatctcaacg ttgatatagc gcccaccttc cagccgctca gcaagcgcag aagctacgcc
     6961 tggcaccgtt cgcgccactt cttcaatttg ctcagccatc gcgtcgatat ccgccagcac
     7021 agtgccggaa actttaatgc cgatggggct tttaatgccg gttgagagca tatcgatacg
     7081 gttacgaatt ggcggcaccc acagattcgc cagccccggc agacgcacgg tgttatccag
     7141 ttcctcaatg attttgtcca ttgtcatgcc tggccgccac tgatcctgtg gcttaagctg
     7201 gatggtcgtt tctaccatct ccagcggagc agaatcggtg gcggtttccg ctttcccggt
     7261 tttgccaaat acccgcgcca cttcaggtac gctcataatt agcttgtcgg ttttttgcag
     7321 catactcgcc gcctctgctg cggaaatccc cggcagcgtc gatggcatat acaacaagtc
     7381 gccttcattg atctgcggta aaaattcccc gccaacttta ttgagcggcc agagaaccgt
     7441 cagcaccgaa agcgccgcca ccagcagcgt ggttttcggc cagtgcagta ctttcagcaa
     7501 cagcggatga taaacacgaa tcaaaaagcg attgagcgga ttactgcttt ccggcggaat
     7561 tttgccacgg atccagtaac ccatcaggat agggatcact acgatcgcca gcagcgccgc
     7621 acccgccatc gcatacgttt tggtgaacgc caacgggcca aacagacgcc cttcctgccc
     7681 ttccagggtg aagatcggga taaacgacaa cgtgataatc agcagactga taaacagcgc
     7741 cggccccact tcaacagacg catcggtgat cacctgccag cgcgttttat tatccagcgt
     7801 ggcgtcagga tgctggtgct gccactcttc cagccgttta tgcgcattct cgatcatgac
     7861 gatagcggca tcgaccatcg ccccgacggc aatcgcaatg ccacccagcg acataatatt
     7921 ggcattcagc ccctggaagg gcatgacaat aaaagcaata cacaacccca gcggcaacga
     7981 aataatcgcc accagcgccg agcgcacatg ccagagaaac ggcgcgcaga ctaccgccac
     8041 cacaataaac tcttccagca acttgccgct gaggttgtcg atggcccggt caatgagctg
     8101 gctgcggtcg tatgttgtaa ctatctccac gccttccggc agactacttt tcagcgtttc
     8161 cagtttgtcc ttcacggcgg cgatcacttc tcgggcgttt ttgccggatc gcaggatcac
     8221 caccccgccc gccacttcgc cttcgccgtt tagttcggca atgccccggc gcatcttcgg
     8281 gccaatctgg accttcgcaa catcgcgcag ataaacgggc acgccatttt cactggcttt
     8341 taaaacgatg tgattaaagt cgtcgagcgt ttgtagatag ccgctggcgc gcaccatata
     8401 ttctgcttcc gccagttcga ttgacgaacc gcccgcttcc tggtttgaag catccagcgc
     8461 gctttttact tcggcgagac tgatgccata ctgcgccagg cgctgaggat cgataaccac
     8521 ctgatactct ttcaccacac cgcctaccga cgccacttcc gcaacgtcag gaatggtttt
     8581 cagctcatat ttgagaaacc agtcctgcaa tgagcgtaaa tcggcaagat cgtgcttacc
     8641 gctgcgatcc accagtgcat attcatagat ccagccaaca cccgtggcat ctggtcccag
     8701 ctcggcgctg actcccgcag gcagcttacc ctgtacctgg ttgaggtact ccagcacccg
     8761 cgaacgcgcc cagtacggat cggtgccatc ttcgaaaatg acatacacat aagagtcgcc
     8821 aaactgcgag aaaccacgca cagtcttcgc gccaggcacc gacaacatgg tggtggttag
     8881 cggataagtc acctgatttt caacgatttg cggtgcctgg ccgggatagc tggttttaat
     8941 aatcacctgc acatcggaga gatccggcaa cgcatccact ggcgtattaa tgatggtcca
     9001 ggtgccccag atgctcagaa acaacgcgcc catcagcacc aaaaaacggt tcgccaccga
     9061 gcgacgaata atccattcaa tcattggtta ttccctcaat gcgcatgggt agcactttca
     9121 gagcgcatcc gctccagtgc gccagaaata ttggcttcgg aatcaatcag gaacaggccg
     9181 ccggaaacca ccttttcacc ttccgccaga ccagagcgta atgcggtgac gccttgcgat
     9241 gcctggaaaa cagcaacgcg tttcggtaca aagcgcccgt cggcatcaac ggtaatcacc
     9301 cgctgttcgt tgccggtatc aatcagcgcc tgtgacggaa tgagcagcat cggttcgctg
     9361 gcggtgttga gttgcaacca ggcgttcatg cccggtttta gcgcctcgtc ggcgttgtcg
     9421 acttccagac gcagctgcag cgtgcgggtc gcggcatcca cgccaggtag cagcgtccat
     9481 ttgcggatgg tgagtgtttt atccggtcgc gccggaacgg tgagggtaaa ctgcgaggca
     9541 tctttcacca gccaggcgat ggactccggg atcgcagcag tgacccacac cgggtccata
     9601 ccctgaattc gcgattacgc gcaatccccc tgagaaccgc catgaacaag ggtttcatga
     9661 tttgtgattt tagtttggta cgcaatttgg tacacaacac tattttcact tcagcgggta
     9721 gtcatcaaac tcaccataac gagcatcgtt gatgatgtat gtgatcacca caaatatcac
     9781 atctggacta gttccgtctt catgcgcagg tagactggcc ccctgaatct ccagacaacc
     9841 agtatcactt atttaagtga tagtcttaat actagttttt agact
//
LOCUS       DAZXUK010000142         9771 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZXUK010000142 DAZXUK010000000
VERSION     DAZXUK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.142"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            390..2021
                     /gene="oppA"
                     /locus_tag="ACH3L8_002709"
     CDS             390..2021
                     /gene="oppA"
                     /locus_tag="ACH3L8_002709"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFT4085761.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2107..3027
                     /gene="oppB"
                     /locus_tag="ACH3L8_002710"
     CDS             2107..3027
                     /gene="oppB"
                     /locus_tag="ACH3L8_002710"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFT4085762.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3042..3950
                     /gene="oppC"
                     /locus_tag="ACH3L8_002711"
     CDS             3042..3950
                     /gene="oppC"
                     /locus_tag="ACH3L8_002711"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFT4085763.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3962..4975
                     /gene="oppD"
                     /locus_tag="ACH3L8_002712"
     CDS             3962..4975
                     /gene="oppD"
                     /locus_tag="ACH3L8_002712"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFT4085764.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4972..5976
                     /gene="oppF"
                     /locus_tag="ACH3L8_002713"
     CDS             4972..5976
                     /gene="oppF"
                     /locus_tag="ACH3L8_002713"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFT4085765.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6029..6358)
                     /locus_tag="ACH3L8_002714"
     CDS             complement(6029..6358)
                     /locus_tag="ACH3L8_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFT4085766.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6393..7853)
                     /gene="cls"
                     /locus_tag="ACH3L8_002715"
     CDS             complement(6393..7853)
                     /gene="cls"
                     /locus_tag="ACH3L8_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFT4085767.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7996..8169
                     /locus_tag="ACH3L8_002716"
     CDS             7996..8169
                     /locus_tag="ACH3L8_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFT4085768.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8224..9477)
                     /gene="kch"
                     /locus_tag="ACH3L8_002717"
     CDS             complement(8224..9477)
                     /gene="kch"
                     /locus_tag="ACH3L8_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFT4085769.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2285 c         2432 g         2491 t
ORIGIN      
        1 aaagatgttt gttaaggcat ggatgcaagc tatagattct gatacggtca ataaaagaga
       61 atcgcttaac aattttgcaa aatgtattgg cgagtaagaa ccgcatttgg tactttccgg
      121 gcaaccgcca gacgattctt tattggtaat gagaataatt aacaattaaa gagcgtcgcg
      181 aaagaataat gtgtctcgac aggggagaca cagtacgaat cgacataagg tgatcgtctg
      241 aatcaccaga ataaataaag tcggtgatag taatacgtaa cgataaagta acctgacagc
      301 agaaagtctc cgagcttgtg cagggtccca atccgggatt acacatgctg gttaatacca
      361 gtaattataa tgagggagtc caaaaaacaa tgaccaacat caccaagaga agtttagtag
      421 cagctggcgt tctggctgcg ctaatggcag ggaatgtcgc gctggcagct gatgtacccg
      481 caggcgtcac actggcggaa aaacaaacac tggtacgtaa caatggttca gaagttcagt
      541 cattagatcc gcacaaaatt gaaggtgttc cggagtctaa tatcagccga gacctgtttg
      601 aaggcttact ggtcagcgat cttgacggtc atccagcacc tggcgtcgct gaatcctggg
      661 ataataaaga cgcgaaagtc tggaccttcc atttgcgtaa agatgcgaaa tggtctgatg
      721 gcacgccagt cacagcacaa gactttgtgt atagctggca acgttctgtt gatccgaaca
      781 ctgcttctcc gtatgccagt tatctgcaat atgggcatat cgccggtatt gatgaaattc
      841 ttgaagggaa aaaaccgatt accgatctcg gcgtgaaagc tattgatgat cacacattag
      901 aagtcacctt aagtgaaccc gttccgtact tctataaatt acttgttcac ccatcaactt
      961 caccggtgcc aaaagccgct atcgagaaat tcggcgaaaa atggacccag cctggtaata
     1021 tcgtcaccaa cggtgcctat accttaaaag attgggtcgt aaacgaacga atcgttcttg
     1081 aacgcagccc gacctactgg aacaacgcga aaaccgttat taaccaggta acctatttgc
     1141 ctattgcttc tgaagttacc gatgtcaacc gctaccgtag tggtgaaatc gacatgacgt
     1201 ataacaacat gccgatcgaa ttgttccaga agctgaaaaa agagatcccg gacgaagttc
     1261 acgttgatcc atacctgtgc acttactatt acgaaattaa caaccagaaa ccgccattca
     1321 acgatgtgcg tgtgcgtacc gcactgaaac taggtatgga ccgcgatatc attgttaata
     1381 aagtgaaagc gcagggcgac atgcccgcct atggttatac tccaccgtat actgatggcg
     1441 caaaattgac tcagccagaa tggtttggct ggagccagga aaaacgtaac gaagaagcga
     1501 aaaaactgct ggctgaagcg ggttataccg cagacaaacc gttgaccatc aacctgcggt
     1561 ataacacctc cgatctgcat aaaaagctgg cgattgctgc ctcttcattg tggaagaaaa
     1621 acattggtgt aaacgtcaaa ctggttaacc aggagtggaa aacgttcctc gacacccgtc
     1681 accagggcac ttttgatgtg gcccgtgcag gctggtgtgc agactacaac gaaccaactt
     1741 ccttcctgaa caccatgctg tcgaacagct cgatgaatac cgcgcattat aagagcccgg
     1801 cctttgacag cattatggcg gaaacgctga aagtgactga cgaggcgcag cgcacagctc
     1861 tgtacactaa agcagaacaa cagctggata aggattcggc cattgttcct gtttattact
     1921 acgtgaatgc gcgtctggtg aaaccgtggg ttggtggcta taccggcaaa gatccgctgg
     1981 ataataccta tacccggaat atgtacattg tgaagcacta atggcaatac gtggggcagg
     2041 agtgtcctgc tccacggtgt ctgattttta tcgcattaca gaaggcacag gccagaaggt
     2101 agggcaatgt taaaatttat tctacgtcgt tgtctggaag cgattccgac gctatttatt
     2161 cttattacta tttcgttctt tatgatgcgc ctcgcaccgg gaagcccttt taccggcgaa
     2221 cgtactttac cgccagaagt gatggccaat atcgaagcga aatatcatct taatgatccg
     2281 atcatgacac agtatttcag ctacctgaaa caactggcgc atggcgattt cggtccatcg
     2341 tttaaatata aagattattc ggtcaatgac ctggtggcat ccagttttcc cgtttctgcc
     2401 aaactgggag ccgccgcatt tttccttgcg gtaatactgg gtgttagtgc tggcgttatt
     2461 gccgcattaa aacaaaacac caaatgggac tataccgtga tggggctggc aatgaccggg
     2521 gttgttatcc ccagttttgt ggttgcgcca ttattagtca tgatatttgc gatcattttg
     2581 cattggctgc cgggcggtgg ctggaatggt ggggcgctta aattcatgat attgccgatg
     2641 gtggcgttgt cactcgccta tatcgccagt attgcgcgta ttacccgtgg ctctatgatt
     2701 gaagtattac actccaactt tattcgtact gcccgggcga aagggttacc tatgcggcgg
     2761 atcattttac gccatgcatt aaaacctgct ctgttacccg tgctctccta tatgggcccc
     2821 gcatttgtcg gcattattac cggttctatg gtcatcgaaa ccatttatgg tttgccgggg
     2881 attgggcaac tgttcgttaa tggcgcattg aaccgtgact attccttagt gttaagcctg
     2941 accatcctgg ttggtgcgtt aaccattttg tttaatgcca ttgtcgatgt gctatatgcg
     3001 gttatcgacc cgaaaatccg ttactgatac tggagctcgc gatgatgtta agtaagaaaa
     3061 acagcgagac gctggaaaat ttcagtgaaa agctggaggt cgaagggcgc agcttgtggc
     3121 aggacgcacg tcgacgtttt atgcataacc gtgcggcggt tgccagtctg atagtgctgg
     3181 tgctgatcgc gttatttgta atcctggcac cgatgctttc ccagtttgcc tatgacgata
     3241 ctgactgggc gatgatgtcc agcgccccgg atatggagtc cggtcactac tttggtactg
     3301 actcatccgg tcgcgacctg cttgtgcgcg ttgcgattgg cgggcgtatc tcactcatgg
     3361 tcggtgttgc tgcggcactg gtggcagtgg tcgtggggac actttatggt tcgctttccg
     3421 gttatctggg cggtaaagtg gattcggtaa tgatgcgtct gctggaaatc ctcaactcct
     3481 tcccattcat gttcttcgtc attttgctgg tgaccttttt cggccaaaac atcctgctga
     3541 ttttcgtggc gattggcatg gtttcctggc tggatatggc tcgtattgtg cgtgggcaaa
     3601 ccctgagtct gaagcgcaaa gagtttattg aggcggcaca agttggcggt gtatcgacgc
     3661 cgggcattgt tattcgccac attgtgccga acgtactcgg tgtggtggtg gtctacgcat
     3721 cgctactggt gcccagcatg atcctctttg aatctttcct tagcttcctg gggttgggta
     3781 cgcaagagcc gttaagcagc tggggggcat tgctgagtga tggcgcgaac tcgatggaag
     3841 tctctccatg gttactgttg ttcccagcgg gattccttgt ggtgacactg ttttgtttca
     3901 actttatcgg cgatggcttg cgtgatgccc tcgacccgaa agatcgttaa ggagtgcagc
     3961 catgagcgta attgaaactg caactgtgcc gctcgcacaa caacaggctg acgcactgct
     4021 gaacgtgaaa gatttgcgtg tcacctttag taccccggac ggcgacgtca cggcggtcaa
     4081 tgatttaaat ttttccctac gtgccggaga aacgctgggc attgtaggtg agtctggttc
     4141 gggtaaatcg caaactgcat ttgcgttgat gggcctgttg gctgccaacg gacgcattgg
     4201 cggatcggca accttcaatg ggcgtgaaat cctcaatttg ccagagcgtg aactcaataa
     4261 actgcgcgct gaacaaatct caatgatttt tcaggaccca atgacttcgt tgaatcccta
     4321 tatgcgcgtc ggtgagcagt tgatggaagt gctgatgctg cataagaaca tgagcaaagc
     4381 tgaggcgttt gaagagtcgg tgcggatgct cgatgcggta aaaatgccgg aagcacgtaa
     4441 acgcatgaaa atgtacccgc acgaattttc tggcggcatg cgtcagcgag tcatgattgc
     4501 gatggcattg ctatgtcgac ctaagttgct gattgcggat gaacccacta cagcgctgga
     4561 cgtcaccgta caggcgcaga tcatgacgct attgaatgaa ctgaagcggg aatttaatac
     4621 tgccatcatt atgatcaccc acgatcttgg cgtggtggcg gggatctgtg acaaagtgct
     4681 ggtaatgtac gccgggcgca cgatggaata tggcaatgcg cgcgatgtct tttatcaacc
     4741 cgttcatcct tattctatcg gtttgctcaa cgcggtgccg cgtcttgatg cggaaggtga
     4801 aacaatgttg accatcccgg gtaatccgcc aaacttgctg cgattaccga aaggttgccc
     4861 gttccagcca cgttgtccac atgcgatgga aatttgtagt agcgcaccgc cgctggaaga
     4921 gtttacgcct ggccgtctgc gtgcttgctt taaaccggtg gaggaactgt tatgaatgct
     4981 gtaactgaag gaagaaaagt cctccttgaa atcgccgatc ttaaagtgca ctttgaaatc
     5041 aaagatggca aacagtggtt ctggcaaccg ccgaaaacgc tcaaagccgt cgatggtgtc
     5101 actcttcgcc tgtatgaagg ggaaacatta ggtgtggtag gggaatcggg atgcggtaag
     5161 tccacctttg ctcgcgccat catcggtttg gtcaaggcga ccgacggtca tgttgcctgg
     5221 ttaggtaaag agttgctggg catgaagccc gatgaatggc gtgccgttcg cagtgatatt
     5281 cagatgattt tccaggatcc gttggcatcg ctaaacccgc gtatgaccat cggcgagatc
     5341 atcgctgaac cactgcgtac ttatcatccg aaaatgtcac gccaggaagt tcgcgagcgc
     5401 gtgaaggcga tgatgctgaa agtcgggtta ttgcctaacc tgattaaccg ctatccgcat
     5461 gagttctccg gtgggcagtg ccagcgtatc gggattgctc gtgctcttat tcttgaaccg
     5521 aagctgatta tctgcgatga gccggtttcg gcgctggacg tgtcaattca ggcgcaggtg
     5581 gttaacctgc tccagcagct gcaacgtgag atgggattgt cattaatttt tattgctcat
     5641 gacctggccg tggtaaaaca catttccgat cgtgtgttgg tgatgtatct cggccatgca
     5701 gtagaactgg ggacctatga tgaggtctac cacaatccac tacatcctta caccagggca
     5761 ttgatgtcgg cagtccccat acctgatccg gatctggaga agaacaaaac catccagtta
     5821 ctggaagggg aattaccgtc gccgatcaac ccgccttccg gttgtgtttt ccgtacccgt
     5881 tgcccgattg ccggtccgga gtgcgccaaa acacgtcctg ttctggaggg gagtttcaga
     5941 cacgccgttt cttgcctgaa agtcgatccg ctttaaaaac aataagggct gacagttgtc
     6001 agcccttttt cacgctaaaa gcgattattt attcccgcca gatgatatgg caaagtttgt
     6061 gatctttttc gcggcataac agaatgcggg cgaaaacatc gctgatttca ccatcttcac
     6121 tgtccgccag accaatcacc acttcggcaa aaaagtccgg gttcaaatcg aaatcaacat
     6181 gttcctgcca gtcttccgcc ggatcgaata actccgcgcc gccgcgctct tcaaactgaa
     6241 gattgaacag cagaacatca gctggatcga gattgtcggc agccagttcg agaaaaatat
     6301 cgtaggcctg ctcaagcgtt tcatcttcag tcaggcgatt gtttagatcc atatccatag
     6361 tcactacctg tttaacctct gttggcgacg ttttacagca acgggctgaa gaagtaaaac
     6421 agtcgctcgg cgacacgttg ccataatgga cgttttagcc ataaacgggc atcgagcaga
     6481 cgtgaacgcg aaatataatc gtcctgaacg gcggcaaggt ctgcaccaaa acctttatcg
     6541 tcgattgcca aggtaatctc gaaattcagc cacagactac gcatatcaag gttaacggtg
     6601 ccaaccagac ttagttcgcc atcgaccagc acgctcttgg tatgcagtaa cccgccttca
     6661 aactgataaa ttttaacccc agcagcaagc agttccgtaa agaatgcgcg actggcccag
     6721 ccgaccagca tcgagtcatt ttttcgcgga aggataatac tgacatccac cccgcgctgc
     6781 gccgccgtgc aaatcgcatg aagtaaatca tcgcttggca caaagtaggg cgtggtcatg
     6841 atcaaatatt catgcgccga ataagccgca gtcaataatg cctggtgaat gagatcttcc
     6901 ggaaagccgg ggccagaagc aattgtgtga atggtgtgac cgctggcctg ttcaaacggc
     6961 ataatattga catctggtgg tggcggcaga atacgttttc cggtttcaat ctcccagtcg
     7021 caggaataaa taatccccat cgcggtggcg atggggcctt ccatacgcgc catcagatca
     7081 atccattgcc ctacgcccgc atcttgtttg aagtagcgag gatcgaccat attcatgctg
     7141 ccggtgtacg cgatgtaatt atcgatcatg atcatcttgc gatgttggcg caggtccata
     7201 cggcgtaaaa acacacgcat cagattgacc tttaaggctt cgaccacttc aataccggca
     7261 ttacgcatta gctcgggcca cgggctgcgg aaaaaagcca cactcccggc ggagtcgagc
     7321 atcaatcggc aatgaatacc acgccgtgca gccgccatta atgattcagc cacctggtcc
     7381 gccatgccgc cgggctgcca gatataaaac accatctcaa tattatggcg cgcgagctgg
     7441 atgtcgcgga ttaacgcctg catcacatca tctgactcgg tcatcagctg tagctgattc
     7501 cctttgaccc cagcgatccc ctgacgacgc tcgcaaagct tgaataatgg cgcagcgaca
     7561 ctgctatttt cttcggcgaa gatatgctta caggctttaa ggtcgttaag ccattttgcg
     7621 gtggaaggcc acatcgctct ggcgcgctca gcgcggcgtt tgcctaaatg gagctcgcca
     7681 acggcaagat aggcaataat tccgactaac ggcagaatgt aaataatcaa cagccaggcc
     7741 atcgcggagg gaactgcgcg tcgtttcatt agaatgcgta aagttacgcc tgcaatgagc
     7801 aaccagtatc ccagaatggc caaccaactc accaacgtat aaacggttgt catagattag
     7861 aaatcctttt gaaagcgcat tgttatgagt ttacgcatat ggattcatct ggcaaataaa
     7921 aacgcgggaa aagcactggt cagcgtcggg ggcgggggta taatgaccat tctgttattg
     7981 catagagtag ttaacatgaa gcggagtaga acggaagtgg ggcgctggcg catgcagcgt
     8041 caggctagcc gacgtaaatc gcgttggctt gaggggcaat cgcgccgaaa tatgcgtatc
     8101 cacagcatca ggaagtgcat tctaaacaaa cagcgtaact cgttattgtt tgcgatctac
     8161 aatatctaaa tgtactgggc accattgcgg tgcccaataa gtttactctt ccgtctttaa
     8221 actctatttt tgcgccgatt ctttactgtc agctttcgtt tcttgttcat cgttatcgct
     8281 gaaaatacca tgacctgagt tcaacaacat tgaaacgagc atatcattat taatctcttc
     8341 accatttaaa actcgcgcca gaatttcgct gccaaacagt tgcggtgaga gaatgatatc
     8401 cggatgtacc atcttaatct tattcaggtt tttgctatca ctgacggcga gaactgtttt
     8461 gacatcactg ctcatatctt ttgccgagag tacaacaaac gcgttatctg catcgttatc
     8521 actcagcgcc agaatggccc ggcaacgatc gattcccgct ttctttaata ctgaactgtc
     8581 attactgtca ccggggataa catcagcgtt atcgcctaag cgttgctcaa gttgcttgat
     8641 atcatcttca ggcaagttgc tgataaccgt tacgttttgt ccgcgttgat tcagttgcag
     8701 gatcgtattg atggcgagaa tcgaatgtcc gcaaacaata aaatgatctt tacgatgcat
     8761 tgtatgattg tttcctttta aaagtttgtt gaatcccccg cggataagcg gaccaaaaat
     8821 tgaggtcata gatgtggcaa aaacggtaat gccggaaata atgaccgaaa tagtgaacaa
     8881 tcgtgctgat tcagaaacag ggacaatatc tccatagccg acggttgaca tggtttctat
     8941 cgaaaaatag aacgcggtca tcaaactttc tattcgcgga ttaaaacctt cgcttaaata
     9001 aagcgcaccg taggttgagt aaaacagtaa tgtcgtgaaa ctaataaatg caaaaattgt
     9061 cccggctgcg gcgctactgt gggagaagtc tttgcgcagt atcagcaaaa acaccagcgt
     9121 aaaaatgcaa aatccaatac taaatttcag ccagggataa aaatgcaggg tgtaaattag
     9181 cgctatcaac aacaaaatga tactgattgc ccatgcgagc tttgcccgga acagtaagcc
     9241 aatggagttc aggacaagaa agacgccgag cataaatagt ggggcgtttg ccagcaagga
     9301 gagatccaac tctgaaaatg ctttgatatg gaaaatatca agcaaattga ccgacatacc
     9361 atagattgtt ttaaaaataa gcaatccatt taaaaagacg gccagcgcga gaatgtcgtg
     9421 ccgtagggtc acccataaat ttgttgctgt ttgtttgaat gtagcccagt gactcactga
     9481 tacctctgta accacaattt tgtttaatta tcttagcgtg cacattttct gaacatacat
     9541 gcagcgtgtg aatgtgttaa acaatagtag atgactttat ccgcagtaat caaaggtttt
     9601 gagtgagcaa ctctgcgtca ggaaagggct tttttcctga acatgctcac atcatataaa
     9661 gaaatacaga taaagttatt atctgcttgt ggtagtgaat gcactgaccg gctataagga
     9721 aaggccaaac aagaacacgg ttgcaaaaac cgtgccctta aatattggat c
//
LOCUS       DAZXUK010000143         9755 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZXUK010000143 DAZXUK010000000
VERSION     DAZXUK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9755
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.143"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(87..860)
                     /gene="uxuR"
                     /locus_tag="ACH3L8_002718"
     CDS             complement(87..860)
                     /gene="uxuR"
                     /locus_tag="ACH3L8_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFT4085770.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1075..2535)
                     /locus_tag="ACH3L8_002719"
     CDS             complement(1075..2535)
                     /locus_tag="ACH3L8_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFT4085771.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(2616..3800)
                     /gene="uxuA"
                     /locus_tag="ACH3L8_002720"
     CDS             complement(2616..3800)
                     /gene="uxuA"
                     /locus_tag="ACH3L8_002720"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFT4085772.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4140..5483
                     /gene="gntP"
                     /locus_tag="ACH3L8_002721"
     CDS             4140..5483
                     /gene="gntP"
                     /locus_tag="ACH3L8_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFT4085773.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5734..6636)
                     /gene="fimH"
                     /locus_tag="ACH3L8_002722"
     CDS             complement(5734..6636)
                     /gene="fimH"
                     /locus_tag="ACH3L8_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFT4085774.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6656..7099)
                     /locus_tag="ACH3L8_002723"
     CDS             complement(6656..7099)
                     /locus_tag="ACH3L8_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4085775.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(7172..7702)
                     /gene="fimF"
                     /locus_tag="ACH3L8_002724"
     CDS             complement(7172..7702)
                     /gene="fimF"
                     /locus_tag="ACH3L8_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFT4085776.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7712..>9755)
                     /gene="fimD"
                     /locus_tag="ACH3L8_002725"
     CDS             complement(7712..>9755)
                     /gene="fimD"
                     /locus_tag="ACH3L8_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFT4085777.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2240 a         2590 c         2465 g         2460 t
ORIGIN      
        1 cctgcaattt acaaagtaat actcattgcg acttcttaat ttaagaaagc gcaaggaacg
       61 tttacccttg cgcttattat aataagtcag gcgtcgactt tatccagcgg ccatgaatca
      121 aagagatagc cgtcaaaata aatatcgtca acgttcgaga attccagcag acgttgctta
      181 acgttttcca gatgctgcca cattgccagc ttcgccgctc gggcatcttt tttgattaac
      241 gcggcgagga tctgtttgtg atcgcccaac cactctttgc gatacaggct gtcatccaga
      301 tggctgtgca actggatcca cattggattg ttttcccgcc actgccagga ctgacggaat
      361 aactccacca gcatgctgtt atgcgttgct tcagcaatgg cgagatggaa ctgcatgtca
      421 ccgctttcgc tgctacccgg cgcgctggaa gccagttcac gctcttccag ttgcaatgcc
      481 tgacgcattt tgacgatatc ttcgcgggta gcctgcagtg cggcaaactc ggcgatgtta
      541 ctttccagta actgacgcgc ctgtaacagc tcaaaaggac ccgcatcatt gcagacgttg
      601 gcatccggac tgtctgtgtt gtggctgcct gagctatcaa gcacatagat accggcaccc
      661 cggcgcactt ccaccagccc tttgatctcc agcatgatca gcgcttcacg taccaccgtc
      721 cgcgtgacat caagcatttc tgcaatttca cgctccggcg gcagccgttc gccaggattg
      781 tacggcgtct ttatgatcag atcgtggatc atcgccccga cttcctggta aggtctttgc
      841 gcagaggtga cagatttcat ggtgttgtgt ccgttaggtg cggttaatca aatttttgcc
      901 ggatgcggcg tgaacgcctt atccggccta caacgggcaa ttttacggcc cccgttaatc
      961 tcaaactata ccattagcac tggaagttgg cacgtacagg tgggcaaaaa aacgaaaact
     1021 ggtcaaccaa atatcagttt gattgaccag taagtctgta gggtaatcag ttagttacag
     1081 cgcagccaca cactcgcgcg caccgcgttc gcacagctgc tgatatgccg acgtcaccgc
     1141 gccaacaaag tcggcattct gcggcagatc atcggcaaaa ataccgctca ggcccagcag
     1201 cgctttcacg cggtctgcgc cctgatactg cgcgttaatc ttctggaact cggccagcat
     1261 cgggtcgacc acgtcaatgg cattaccctg ctcatccacg ccctgggtgt aacccatcca
     1321 gccagccacg cccagcgcca ggtgacgcca gctgccgccg ttttgcaggt gcagacgcac
     1381 cgggtccagt agacgctgcg gtaacttctg gctgccgtcc atcgcaatct gccaggtacg
     1441 gtgacgcaga gacgggttgc tgaaacgctc gatcagcagc gtcgcatagg cgttcaggtc
     1501 cgtaccttcc ggcatcgaca gcgttggcgc ttgttcctgc atcatcaggg caaaggccgc
     1561 tttgcgataa tccgggttag tcatggtgtc ggcaatggtt tcatagccgc cgaggtaacc
     1621 gaggtacgcc aggaaagagt ggctgccgtt cagcatacgc agcttcatca tttcgaacgg
     1681 cacaacgtct gcaacgaact gtgtgcccac tttatcccag tccgggcgac cattaacgaa
     1741 gttatcttca atcacccact gacggaacgg ttcgcaggca atggcgcacg ggtcgtaaac
     1801 acccagctgg tcagcaattt cctgtaaggt ttctggcgtc gccgccggaa cgatgcggtc
     1861 aaccatggtg cacgggaagg tgacgttctc ttcaatccat gccgctagct gcgggtcacg
     1921 cgcctgagcc agccccagta ccgcgacctt cgccacatgg ccgttttcac gcacgttatc
     1981 gcaggacatc accgtaaatg ctttcagccc tttttcacga cgcagacgca aggcttcgac
     2041 gatgtaacca atcgcggact tcggcgcagt cgggttttcc agatcgtgct tgatcagcgg
     2101 gttattgaga tccagttgac cgcttgccgc atcagcgcag tagccttttt ccgtgaccgt
     2161 tagagagaca atcgccgttt gcggacgcgc catcgcgttg agaatacctt cgcagccgtc
     2221 gatttccgga tgcagcgctt ctttcatcga accgataatt ttcagctcgg tgctttctgc
     2281 gcctttttcc gctacggtgt acagcagttg ctgttttttc aggttttcga tcagcatgcg
     2341 gtcgttgcct ggcatcaggt taacttcgca gatgccccag tcgctgtcgg tgctttccag
     2401 cagatgatgg gtatacagcg cctggtgcgc gcggtgaaac gccccgcaac cgagatgcac
     2461 aatgcgtgat tccagacgag aatgatccca tgacgggcgg gcaaccggca gattgctgtc
     2521 aacaatagta gtcatcgtaa actccagaat ccgtcttcag agagtgacgg caaggaagaa
     2581 ggaaccggca cggcgcgcag ccatgccggt gggtattaac ggctaaagaa agcgcgctgg
     2641 atcgccagtt cgacaccgcg aacttcggcc aggcctttca gacgaccaat tgcggagtaa
     2701 ccagggttgg ttttcttctt caggtcgtcc agcatctgat gaccgtggtc cggacgcatc
     2761 gggatcaggt cttctttgcc ttccgcttta cgacggtgtt cttcttcaac aatcgctttc
     2821 accactttgt acatatcaac gtcaccgttc aggtgcgccg cttcgtggaa ggttttcggg
     2881 ttatcttcac gcatggtgga gcgcagatgg gtgaagtaaa tacgcggacc gaactgcttg
     2941 atcatatcaa ccagatcgtt gtcagcacgc acgccgtagg aaccggtgca catggtgaaa
     3001 ccgttcgcca tgctgtttac ggtatcaacc atccactgca tatcttcaat ggtggaaaca
     3061 atgcgcggca agccgaggat cgggcgcggc ggatcgtccg ggtgaaccgc catacgcacg
     3121 ccaacttctt cagcaactgg aataatcgct ttcaggaaga cggcaaagtt ttcgcgcagt
     3181 ttggctttgt cgatatcttt gtacagctcc aggtgcttac ggaactggtc gagggtatac
     3241 ccttcttccg cgcccggcag accggcaatg atgttacgcg tcagacgcgc tttgtcttcg
     3301 tcgctcatag tggcgaagcg ttcagctgcc tgagcaattt cttcttcggt gtaatccgct
     3361 tccgcgcctg gacgtttcag gatatgcatt tcgaatgcag cgaattcgat ctggtcgaag
     3421 cgcagagctt tggagccgtc tggcagcacg tattcgaggt cagtacgggt ccagtcgagc
     3481 accggcatga agttgtagca cacggtgcga atgccacact gcgccaggtt gcgcagagtc
     3541 tgctgatagt tagcgatcca ctgctcatag ttgccagtgt gggttttgat atcttcgtga
     3601 attggtacgc tttctacgac agaccacacc aggcctgcgt cttcaacgat cgccttgcgt
     3661 ttgaggatct cttctacgga ccatacttcg ccgttcggga tatggtgcag cgcggtaacc
     3721 acgccagttg cgcccgcctg acggacatca gctaaagaaa ccggatcgtt tgggccgtac
     3781 cagcgccagg tctgttccat gatttttcct ctttcacata gtgggttcca cagcaaagag
     3841 acgcggaacc ttggagttgt ttaggtatcc ggatgaacaa ccagaccgca gccgtggcgt
     3901 tgcatcggat gagatggcgt ataagttcta ccttttggta agacatgtca accagaattc
     3961 tgaaattggt taaccgcgtc gcaagaattt cactctcatg gttgttgctg caaattgcag
     4021 caaagggtaa tcgatggtta ttgatcagag gatgtgacat tcatcgcaac aatggttgac
     4081 caatttacat aacatgtcgc ccaaaataac aacggttcaa ccaagtaagg taaaacgtta
     4141 tgcatgtgct taacattctc tgggtggtat tcggcattgg cctgatgctg atactgaatt
     4201 tgaagttcaa aatcaattca atggtggctt tgttggtggc ggcgctgtcc gtcgggatgc
     4261 tggcaggcat ggatttgatg tcgctgctgc acaccatgaa agcgggcttc ggcaacacgc
     4321 tgggagaact ggctatcatc gtggtgttcg gtgcggtcat cggtaaattg atggtcgact
     4381 ccggcgcggc tcaccagata gcacatacgc tgctggcgcg tctcggtctg cgctatgtac
     4441 agctgtcggt gattatcatc ggccttattt tcggtctggc gatgttctat gaagtggcct
     4501 ttatcatgtt agcgccgctg gttattgtta ttgccgccga agctaaaatt ccgttcctga
     4561 aactggcgat cccggcagta gcagctgcca ctaccgcaca ttcactgttc ccaccgcagc
     4621 cgggtccggt ggcgctggtg aatgcttatg gcgcggatat ggggatggtt tatatctatg
     4681 gcgtactggt gacgatccca agtgtaatct gcgcaggtct gatcctgccg aagttcctcg
     4741 gcaatcttga gcgcccaacg ccatcattcc tgaaagcaga tcaaccggta gatatgaata
     4801 atctgccctc tttcggcgtt tcgattctgg tgccgctgat cccggcgatc attatgatct
     4861 ctaccaccat cgccaatatc tggctggtaa aagatacccc tgcctgggaa gtggttaact
     4921 ttattggttc ctcgccgatt gcgatgttta ttgcgatggt ggttgcattc gtactctttg
     4981 gcaccgcgcg tggtcatgac atgcagtggg tgatgaacgc ttttgaaagc gcggtgaaga
     5041 gtattgcaat ggtgattctg atcatcggtg cgggtggcgt gctgaagcag accatcatcg
     5101 acaccggcat tggcgacacc atcggcatgt tgatgtccca cggcaatatc tcgccctaca
     5161 ttatggcatg gctgatcact gtgctaattc gtctggcgac gggtcagggt gtcgtttcgg
     5221 cgatgaccgc cgccgggatt atcagtgctg caatccttga tccagcaacc ggtcagctgg
     5281 ttggcgtgaa tccggcgctg ctggtactgg cgacggctgc gggttccaac accctcaccc
     5341 acattaatga tgcatctttc tggttgttca aaggttactt tgacctgtca gtaaaagaca
     5401 cgttgaaaac ctggggtctg ctggagctgg tcaactccgt ggttgggctg attattgtgc
     5461 tgattattag catggtagcg taaaagaaca ccagagcctg ccaatggcag gctcagactg
     5521 atgactaaag tctaaaaatg tgcccggaca gtcccctcgc ccctccgggg agagggttag
     5581 ggtgagggga acagaccagt aaagtgcgct ttgtttatgc cagatgcgac gctgacgcgt
     5641 cttatctggc ctacaaaggg ctaacgtgca ggttttgtag gtctgatagc gtagcgcctc
     5701 aggtaccagc attagcaatg cgctgtaatt tttttattga taaacaaaag tcacgccaat
     5761 aatcgattgc acattccctg cagtcacctg ccctccggta cgtgcgtaat ttgccgttaa
     5821 tcccagactt accgccgaag ttcctactgc tcctaacgat accgtgttat tcgctggaat
     5881 aatcgtaccg ttgcgcgtca actgtacgcc gacgccctgc gctggtgaaa acgacgcggt
     5941 attggtgaaa atcgagttgc ccgcatctgc ggttgtgccg gagaggtaat accccaggtt
     6001 ttggcttttc gcacaataaa cggtaagagg aattggcact gaaccagggt agtccggcag
     6061 agtaacggtg acatcacgag cagaaacatc acaaccgcca gtgggcacca ccacatcatt
     6121 attggcgtaa atattccaca caaactgaaa atcatcgctg ttatagttgt tggtctgtcg
     6181 caaaataagc acggcaatta atgaaccagc tttaatagcc accccgcccg cactgctcac
     6241 aggcgtcaaa taaagcgcca ccggccacgg cttatccgtt ctcgaattat aaacaacccg
     6301 cggcgtttcg ctggtggtcg gaaatggata gctactgcca ctatatttta cggtcccgga
     6361 aaaattagat aacacgccgc cgtaagccga gcctcgttgc agtgtgacat agtctgtaat
     6421 ggtttccgga taatcgttat ggcaaaagat ttgcgtcgaa agatctacga ccaggttttg
     6481 ccccacattc acgacaggcg caaggtttac ataaacatta gcgctgccac cgccaatagg
     6541 gatagcggta ccattggcgg ttttacaggc gaatgaccag gcatttaccg accagcccat
     6601 cagcagtaca gcaaacaggg taatagctcg tttcattaca attatctctt cgggttcagc
     6661 tgtaggtata ggtgatgcta atcactgcct gaatggttcc ctgagtggct ccgccattta
     6721 ccgtcaatgc tctgacctgt aacgggaagt gcgctgattg tgaggaatca tccacctgaa
     6781 ctgttttggt tgcgccagta ttcaatgtgt tgccactgtc atcctgtagc tctaactgga
     6841 tgttttgcgc ggtcccctgg tttttataat atccggtgct gtcggctgcc ccgctgaagc
     6901 tggcagtgac cctcgacgtt cccaccggac aattagtcaa ctcaagcgca acatcatgcc
     6961 aggccgatgc cgccccggca gacataagac tgaaagaata aagatcgccg agatctaccg
     7021 tggcattggt ggtggaaacc gtacacggtt tggcgacgac cttaccgttc accgtgatgg
     7081 tgacatcggc tgcctgtatc gtcgcacttg cgagcgccaa cattgccgcc aatacatacc
     7141 cacgtttgca ccatttcatg agcatctcca gttactgata ttcaagagtg aaggtagccg
     7201 tagcattgat atgccccgca gtgacaggca cctgtgtcgc cattagccgg gcgtaaaaat
     7261 tcagcgtatt tggtttaccc ggcgtcaggg tcgtccacga aatcgcagac gatggagcat
     7321 taaggggtat ttgattttgc tgctcattca gaagctgtat tcccagtccc gcagccgctg
     7381 acaccgtatt ttcaagtgca agcaggttgg cattgtggct atctgcaacg ccggtaaacc
     7441 caacttttac ggcagaaacg gcattaccac agggtgacag caaaatacga aatggaacga
     7501 caggagtcgt cgcgccaatg ttgttaaatt gcttcgccgc gttttccatc agatcaacag
     7561 taaaattggt tgattcagcg gccacactac agccgttatc tctgacatag ccgcggatag
     7621 taatcgtgct atccgcagcc aaaacgcgac ttaccgccag ccacaaaaaa gcgcacagaa
     7681 gataaaaagg tttgtttctc atcacgcccc cttaacgaca ttcagctgat agctgggtta
     7741 ataactgctg ctgactctct ggtggcagtt gataattggc gacacagtga gcattttcct
     7801 cttctcccca tttcacctga acttttcccg ctaaaggcat tccgctgagg taaacctgac
     7861 cattatccgc aacaatgccg ctactctggc tactctctga tgtcaccatc gccccaaacg
     7921 gcagcggctt attattgtga gttagcgtca tgagcagttt tatcccaacg cgcgctttaa
     7981 actctgctcg cacgatcgcc ccacgagtgg gaacaacgtt agcgaccgcg ttatctaaat
     8041 cgacgttatc agccaaggta ttggtatcca gcgccactct attttcccga tattcagtgg
     8101 cataaggcag cacggcataa ccacgccagt cggtacgcac ccccgtctgg ttttcgactt
     8161 ttgcatcttt tgcgccaggc gctttaacaa gcaccaccgt atcgtttaac ggctgcccca
     8221 gcgttacgcc attggcatga gccaataccc caccgctgac tccgtaatag agctgcttaa
     8281 tatcatcgct atggctgtaa ccgatattgg cattgccgta accaccgcga taattcagcg
     8341 tggcgtagcc tgtacttccg ctattaccat cgcctccccc ggcatagccg gtttgcacgc
     8401 tataactgag gttgttgtct tccagcaacg taccgtatac accagccaga ttggtcatcc
     8461 gaccgttgag atcgtgtgac atgctgtagc tggcactggc atgtcgccac tgagatttac
     8521 tgtcagaacg cagccagtgg ctgaaaggaa tattgacgtt aagcgctaac atctgatccc
     8581 gtcctttttg ccaggcgttt ttcgtcaggc tatagctgag cgtccagttg atatcttcga
     8641 acgcagtatt taatccagcc tggaattgct catcgacatt actcgttccc caataagttt
     8701 gatggctacc actcaaatac agtgttgatg tgcgcccgag ttgctgagta acggtgagct
     8761 gtaatttccc gcgtttgtta taagcgaggt tgtaatagtc ggtgaatttc ggcttaacct
     8821 gaataactcc atcctgtgtt tcgatgttgt agccattcat tcgactgtat gttgtatcag
     8881 cgaaattaaa atatccgctg gtcgaataac ggtaacccac taactgaata ttcgtgcctg
     8941 attcattgag cgatttgtta tagagaaaac gcaccgattg tccgtcatgc tgactgtcat
     9001 cgggaagtgt ggaattagcc tgcgtcatat ccacagacag agcgcccagt gcctccatgt
     9061 ttttcccgat accgaaatta aaagcacgat aacgatccgc cagttgcgtt ccaccatata
     9121 ttgtccagcc agccggaagg ccgtggagta atgtactctg gaaaaagcgg ggtttttcct
     9181 gctgcgcatt tccactacgg tattctcctg ccgtaatgga ataacgagta tgcccttcac
     9241 gttgcaaaag cgggactgac gaatagggta cggtaaaaat ctgcgtgctg ccgtcagcct
     9301 ctttgatcgt tacctgcaag tcaccactat tacctgcggc atagatatcg ttgatggtaa
     9361 aaggccccgg tggcaccgta ctattataaa tgccatatcc attttgttta atagtgacct
     9421 gtgcagtacc acgagcaata ccgtggatca ccggggcaaa tcctctttgg ctatcgggta
     9481 acatattgtc atctgaggcc aattgtgcgc cgcgaaagtt aataccatcg aaaatatcgc
     9541 cctgggtata accatcaccc aacgtcagcc gggaacgtaa aggaattatg tctcgctcaa
     9601 tccaggtaat gatatgctgc catttatttt tgctacctga tgaactgtcg ctactgttat
     9661 aactccaggt ggtattgtcg cgtaaacgcc acgcaccaat atttaaccca ctctgtaggt
     9721 ttaaatatgc ataatggctg ttacccccaa tccga
//
LOCUS       DAZXUK010000144         9740 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZXUK010000144 DAZXUK010000000
VERSION     DAZXUK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9740
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.144"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..663)
                     /gene="rarD"
                     /locus_tag="ACH3L8_002726"
     CDS             complement(<1..663)
                     /gene="rarD"
                     /locus_tag="ACH3L8_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFT4085778.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGI"
     gene            complement(715..1182)
                     /gene="yigI"
                     /locus_tag="ACH3L8_002727"
     CDS             complement(715..1182)
                     /gene="yigI"
                     /locus_tag="ACH3L8_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFT4085779.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1347..2216
                     /gene="pldA"
                     /locus_tag="ACH3L8_002728"
     CDS             1347..2216
                     /gene="pldA"
                     /locus_tag="ACH3L8_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFT4085780.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2349..4178
                     /gene="recQ"
                     /locus_tag="ACH3L8_002729"
     CDS             2349..4178
                     /gene="recQ"
                     /locus_tag="ACH3L8_002729"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFT4085781.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4242..4862
                     /gene="rhtC"
                     /locus_tag="ACH3L8_002730"
     CDS             4242..4862
                     /gene="rhtC"
                     /locus_tag="ACH3L8_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFT4085782.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(4924..5544)
                     /gene="rhtB"
                     /locus_tag="ACH3L8_002731"
     CDS             complement(4924..5544)
                     /gene="rhtB"
                     /locus_tag="ACH3L8_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFT4085783.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5655..6677
                     /gene="pldB"
                     /locus_tag="ACH3L8_002732"
     CDS             5655..6677
                     /gene="pldB"
                     /locus_tag="ACH3L8_002732"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFT4085784.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6685..7485
                     /gene="yigL"
                     /locus_tag="ACH3L8_002733"
     CDS             6685..7485
                     /gene="yigL"
                     /locus_tag="ACH3L8_002733"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFT4085785.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7561..8460
                     /gene="bioP"
                     /locus_tag="ACH3L8_002734"
     CDS             7561..8460
                     /gene="bioP"
                     /locus_tag="ACH3L8_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFT4085786.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8348..9301)
                     /gene="metR"
                     /locus_tag="ACH3L8_002735"
     CDS             complement(8348..9301)
                     /gene="metR"
                     /locus_tag="ACH3L8_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFT4085787.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9537..>9740
                     /locus_tag="ACH3L8_002736"
     CDS             9537..>9740
                     /locus_tag="ACH3L8_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085788.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYD"
BASE COUNT         2262 a         2476 c         2617 g         2385 t
ORIGIN      
        1 aataccggcg gcgatcagca gtaaattcag cgacatcggg ttttgcccca tatggctggt
       61 tgaactgtcg gcaatagcaa acaggtaaat cgccgccacg ggcagcagcc acatggtttc
      121 aattaacatg ccggtttgtg cttctaccgc aatcttcttg cgcaccaggc cgtagaaggc
      181 aaaactaaat gccagcccca gcgcgataat aggtagcgaa ccaaaagtcc acagctggac
      241 caacacgcca catatcgcca gaatcaccgc cagccattgc atccggcgga atcgctcgcc
      301 gaggaaaatc atccccagca caatgttcac cagcgggtta ataaagtaac caaggctcgc
      361 ttccagcata tggtgattgt tcaccgccca gataaacagt agccagttgc caccaatcag
      421 tacggcagag actgccagca taaaaatttt ctgtggcgtc tgaatcagcg tttttaaata
      481 ggaccactgg cggcaaatgc tcatcagcac caccataaag aaaaacgacc agatcacgcg
      541 atgcgtcagg atctcatcgg cgggcacgta gtaaatcagc ttgaagtatg ctggcgcaat
      601 accccaaata aaataagcgg caagagcgag taatacgccc tgccgcgttt gttttgcatc
      661 catcgggaat actcattttt aattggtaac agcagtttac ctgcttttat gtcttcaacc
      721 taccatatag gtggcggtgg cactggcaat ataaagctgt tcttcattgt gtaattcaac
      781 gcgggcgacg gcgactttat tgcctgcacg caacaggcta ctggtagcag taaaacgctc
      841 gcccctgcct gggcgcagat aatcaacgcg aagatcaatg gtccccatcc gcgatagccg
      901 ctggcgtagt tcatcttcac tgatggtttc gtggcgggtt aaggtacttc ccacgcacac
      961 cagaccggcg gcgacatcca gcgccgacgc aatgactccg ccgtgcaaaa tgctttgcgc
     1021 ccagttaccc accatcatcg gctgattttt aaaggccagc tgtgcgaact ccttttcgta
     1081 acgctccagt tccatcccca atgcgcggtt aaatggcatg tgataaacaa acatctcacc
     1141 cactaatttc agggcttgtt cagcggtcag tacggcagac atatcatcct tacacttcat
     1201 tggttaatga aatgttgatt ttatgcttct ttgttgttgg tttctacttt aggaagggat
     1261 aactaacggc aatggagtta agtatgtaaa atagcccgca gaaaaacatt caccttatca
     1321 ataattcgtt acggagaaca cgaccgatgc ggactctgca gggctggttg ttgccggtgt
     1381 ttatgttgcc tatggcagta tatgcacaag aggcaacggt gaaagaggtg catgacgcgc
     1441 cagcggtgcg tggcagtatt atcgccaata tgctgcagga gcatgacaat ccgttcacgc
     1501 tctatcctta tgacaccaac tacctcatct acacccaaac cagcgatctg aataaagaag
     1561 cgattgccag ttacgactgg gcggaaaatg cgcgtaagga tgaagtaaag tttcagttga
     1621 gcctggcatt tccgctgtgg cgtgggattt taggcccaaa ctcggtgttg ggtgcgtctt
     1681 atacgcaaaa atcctggtgg caactgtcca atagcgaaga gtcttcaccg tttcgtgaaa
     1741 ccaactacga accgcaattg ttcctcggtt ttgccaccga ttaccgtttt gcaggttgga
     1801 cgctgcgcga tgtggagatg gggtataacc acgactctaa cgggcgttca gacccgacct
     1861 cccgcagctg gaaccgcctt tatactcgcc tgatggcaga aaacggtaac tggctggtag
     1921 aagtgaagcc gtggtatgtg gtgggtaata ctgacgataa cccggatatc accaaatata
     1981 tgggttacta ccagcttaaa atcggctatc acctcggtga tgcggtgctc agtgcgaaag
     2041 gacagtacaa ctggaacacc ggctacggcg gcgcggagtt aggcttaagt tacccgatca
     2101 ccaaacatgt gcgcctttat actcaggttt acagcggcta tggcgaatcg ctcatcgact
     2161 ataacttcaa ccagacccgt gtcggtgtag gggttatgct aaacgacttg ttttgatgaa
     2221 cggttgagtg gttggcaaat ctggaatcca gcatccagga ttaccctctc agagactaaa
     2281 agcattgcag tttctcgcgc aggcgctgaa aatagcgcct gtttttattt caggcaatcg
     2341 gggtgaatgt ggcgcaggcg gaagtgttga atctggagtc cggagctaaa caggttttac
     2401 aagaaacctt tggctaccaa cagtttcgcc ccggccagga agaaattatc gacactgtgc
     2461 tttccggtcg cgattgcctg gttgttatgc ccactggtgg cggaaaatcc ctttgctatc
     2521 aaattcctgc cttattgcta aacggcctta ccgtggttgt ttcaccgctg atttcgttga
     2581 tgaaagatca ggtggatcaa ctacaggcca acggcgtggc ggcggcgtgc cttaactcga
     2641 cgcaaacccg cgaacagcaa cttgaagtga tgacaggctg ccgcaccggg caaattcgct
     2701 tactgtatat cgccccggaa cgcctgatgc tggataactt tcttgagcat ctggcgcact
     2761 ggaatccggt gttattagcc gtcgatgaag cgcactgtat ctcccaatgg ggtcacgatt
     2821 tccgcccgga atatgccgcg ctcggtcagt tgcgccagcg gttcccgacg ctgccgttta
     2881 tggcgctgac cgccacagcc gacgacacca cgcgccagga tatcgtgcgc ctgctggggc
     2941 tgaacgatcc gctgattcaa atcagcagtt ttgaccgtcc gaatattcgc tacatgctga
     3001 tggagaaatt caaaccgctc gatcagttga tgcgctacgt gcaggaacag cgcggtaagt
     3061 ctggcattat ctactgcaac agccgggcga aagtagaaga caccgctgcg cgcctgcaaa
     3121 gcaggggtat tagcgcggcg gcctatcatg ccgggctgga aaataatgtc cgcgccgacg
     3181 tgcaggagaa attccagcgc gatgatctgc aaattgtggt ggcgacggtg gcgttcggca
     3241 tgggcatcaa taaaccaaac gttcgcttcg tggtccactt tgatattccg cgcaatatcg
     3301 aatcctatta tcaggaaacc ggtcgcgccg ggcgtgatgg cctgcctgcg gaagcgatgc
     3361 tgttttacga tccggctgat atggcgtggc tgcgccgttg tctggaagag aagccgcagg
     3421 ggcagttgca ggatatcgag cgccacaaac tcaatgcgat gggcgcgttt gccgaagcgc
     3481 aaacttgccg tcgtctggta ttgctgaact attttggcga agggcgtcag gagccgtgcg
     3541 ggaactgcga tatctgcctc gatccgccga aacagtacga cggttcaacc gatgctcaga
     3601 ttgccctttc caccattggt cgtgtgaatc agcggtttgg gatgggttat gtggtggaag
     3661 tgattcgtgg tgctaataac cagcgtatcc gcgactatgg tcatgacaaa ctgaaagtct
     3721 atggtatggg ccgtgataaa agccatgaac attgggtgag cgtgatccgc cagctgattc
     3781 atctcggcct ggtgacgcaa aatattgccc agcattctgc cctacaactg acagaggccg
     3841 cgcgcccggt gctgcgcggc gaatcctctt tgcaacttgc cgtgccgcgt atcgtggcgc
     3901 tcaaaccgaa agcgatgcag aaatcgttcg gcggcaacta tgatcgcaaa ctgttcgcca
     3961 aattacgcaa actgcgtaaa tcgattgccg atgaaagtaa tgtcccgccg tacgtggtgt
     4021 ttaacgacgc aaccttgatt gagatggctg aacagatgcc gatcaccgcc agcgaaatgc
     4081 tcagcgttaa cggcgttggg atgcgcaagc tggaacgctt tggtaaaccg tttatggcgc
     4141 tgattcgtgc gcatgtcgat ggcgacgacg aagagtagtc agcagcataa aaaagtgcca
     4201 gtatgaagac tccgtaaacg tttcccccgc gagtcaaatg tatgttgatg ttatttctca
     4261 ccgtcgccat ggtgcacatt gtggcgctta tgagccccgg tcccgatttc ttttttgtct
     4321 ctcagaccgc tgtcagtcgt tcccgtaaag aagcgatgat gggcgtgctg ggcattacct
     4381 gcggcgtaat ggtttgggct gggattgcgt tgcttggcct gcatttgatt atcgaaaaaa
     4441 tggcctggct gcatacgctg attatggtag gcggtggcct gtatctctgc tggatgggtt
     4501 accagatgct acgcggtgca ctgaaaaaag aggctgtatc tgcgcctgca ccacaggttg
     4561 agctggcgaa aagcgggcgc agtttcctga aaggcttact gaccaatctc gccaatccga
     4621 aagcgattat ctattttggt tcggtattct cattgtttgt cggcgataac gttggcacta
     4681 ccgagcgctg gggcattttc gcgttgatta ttgtcgaaac actggcgtgg tttaccgtcg
     4741 ttgccagtct gtttgccctg ccgcaaatgc gccgtggtta tcaacgtctg gcgaagtgga
     4801 ttgatggttt tgccggggcg ttatttgccg gatttggcat tcatttgatt atttcgcggt
     4861 gatgccagac gcgtcttcag agtaagtcgg ataaggcgtt tacgccgcat ccgacattat
     4921 ttttcacgca tgcctcgccg atgctaacag cgctcccacc agcataaaca acgagccgaa
     4981 aatcttattc agcgccttca tctgctttgg tcctttaatc catagagcaa tcctttgagc
     5041 aagggtggcg taaccgatca tcacaataat atcgaccaca atagtggtga cgccgagcac
     5101 gatatactgc atcagttgcg gctgttgcgg catgatgaat tgcggaaata gcgccgccag
     5161 aaacacaata cttttgggat tggtgagatt cacaaaaact gcgcgctgga acaaatgtcg
     5221 acgcgattga gtagaggcca gcgatttaag atcaattgcg ccagcggcgc gccactgctg
     5281 gattcccagc caaatcaagt aagccgcgcc tgcccacttt aacacttcaa acgcaatcac
     5341 tgagcgggaa aatagcgtcc ccaaccccac gccaaccagc acaatatgaa tcgccagtcc
     5401 ggtctgaagc ccagcaatag acgccaccgc gccgcgataa ccgtggttga gcgaggtggt
     5461 catagtgttg attgcaccag agcctggcga caggcttaaa atgatcgatg tcagcaggta
     5521 ggcaaaccac cattctaagg tcatgatgaa ctcccggtgt gtctattttt gtgccacaat
     5581 acgctactgt cgcagcgttg tgtcaggcac gcttaaaaaa acgattttac gtggtttaag
     5641 aggcagatta cccgatgttt cagcagcaaa aagactggga aacaagagaa aacgcgtttg
     5701 ctgcttttac catgggaccg ctgactgatt tctggcgtca gcgtgatgaa gcagagttta
     5761 ctggtgtgga tgacattccg gtgcgctttg tccgttttcg cgcacagcac catgaccggg
     5821 tggtagtcat ctgcccgggg cgtattgaga gctacgtaaa atatgcggaa ctggcctacg
     5881 acctgttcca tttggggttt gatgtcttaa tcatcgacca tcgcgggcag ggacgttccg
     5941 gtcgcctgtt agccgatccg catctcgggc atgttaatcg ctttaatgat tatgttgatg
     6001 atctggcggc attctggcag caggaggttc agcccggtcc gtggcgtaaa cgctatatac
     6061 tggcacattc gatgggcggt gcgatctcca cattatttct gcaacgccat ccaggtgtat
     6121 gtgacgccat tgcgctaact gcgccaatgt ttgggatcgt gattcgtatg ccgtcattta
     6181 tggcacggca gatcctcaac tgggccgaag cgcatccacg tttccgtgat ggctatgcaa
     6241 taggcaccgg gcgctggcgc gcgttgccgt ttgctatcaa cgtactgacc cacagcagac
     6301 agcgatatcg acgtaactta cgcttctatg ctgatgaccc aacgattcgc gtcggtgggc
     6361 cgacctacca ttgggtacgc gaaagtattc tggctggcga acaggtgtta gccggtgcgg
     6421 gtgatgacgc cacgcaaacg cttctcttac aagctgaaga ggaacgcgtg gtggataacc
     6481 gcatgcacga ccgtttttgt gaactccgca ccgccgcggg ccatcctgtc gaaggaggac
     6541 ggccgttggt aataaaaggt gcttaccatg agatcctttt tgaaaaggac gcaatgcgct
     6601 cagtcgcgct ccacgccatc gttgattttt tcaacaggca taactcaccc agcggaaacc
     6661 gctctacaga ggtttaaatt tcttatgtac caggttgttg cgtctgattt agatggcacg
     6721 ttactttctc ccgaccatac gttatcccct tacgccaaag aaactctgaa gctgctcacc
     6781 gcgcgcggca tcaactttgt gtttgcgacc ggtcgtcacc acgttgatgt ggggcaaatt
     6841 cgcgataatc tggagattaa gtcttacatg attacctcca atggtgcgcg cgttcacgat
     6901 ctggatggca atctgatttt tgctcataac ctggatcgcg acattgccag cgatctgttt
     6961 ggcgtagtca acgacaatcc ggacatcatt actaacgttt atcgcgacga cgaatggttt
     7021 atgaatcgcc atcgcccgga agagatgcgc ttttttaaag aagcggtgtt caaatatgcg
     7081 ctgtatgagc cgggattact ggagccggaa ggcatcagca aagtgttttt cacctgcgat
     7141 tcccatgaac aactgctgcc gctggagcag gcgattaacg ctcgctgggg cgatcgcgtc
     7201 aacgtcagtt tctctacctt aacctgtctg gaagtgatgg cgggcggcgt gtcgaaaggc
     7261 catgcgctgg aagcagtggc gaagaaactg ggctacagcc tgaaggattg tattgcgttt
     7321 ggtgacggga tgaacgacgc cgaaatgctg tcgatggcgg ggaaaggctg cattatgggc
     7381 agtgcgcacc agcgtctgaa agatctgcat ccggagctgg aagttattgg tactaatgcc
     7441 gacgacgcgg tgccgcatta tctgcgtaaa ctctatttat cgtaatcgtt ctttatttgg
     7501 tcagttgtca acctgatact tcgctacaat ggatactcgt taatcaaaga gttttccatt
     7561 gtggcgttac ttatcatcac cacgattctg tgggccttct cctttagctt ttatggcgag
     7621 taccttgcgg ggcacgtcga tagctatttt gcggtgctgg tgcgcgttgg cttggcggca
     7681 ctcgtttttc tgccgtttct gcgtacccgt agcaatagcc tgaaaacggt cggcctgtat
     7741 atgctggtgg gcgcgatgca gctcggcgtg atgtatatgc tgagtttccg cgcttatctc
     7801 tacctgacgg tctccgaatt gttgctgttc accgtactga cgccgctcta catcacgctg
     7861 atttatgaca tcatgagtaa gcgccgtctg cgctggggct atgcttttag cgccttgctg
     7921 gcggtgattg gtgccgggat tattcgctat gatcaggtca ccgaccattt ctggactggc
     7981 ttgttgctgg tgcaactctc caatatcact tttgccatcg gcatggtggg ttacaaacgc
     8041 ctgatggaaa ctcgcccgat gccgcagtat aacgcctttg cgtggttcta tcttggcgcg
     8101 ttcctggtgg cggtaattgc atggttcttg ctgggaaatg cgcagaaaat gccgcaaacc
     8161 acgttgcaat ggggcattct ggtgtttctt ggcgtggtgg cttccgggat tggctacttt
     8221 atgtggaact acggcgcgac gcaggtggac gccggaacgc tgggcattat gaataatatg
     8281 cacgttccgg cagggctgct ggtaaacctg gctatctggc accaacagcc gcactggcca
     8341 acgtttatta caggcgcgct ggtgatcctg gcctcactgt gggtgcatcg taagtgggtc
     8401 gctccgcgct cttcacaaac ggcagatgat cgcaggcgtg attgcgcgct gagcgaataa
     8461 acgcttccgt aactggctga cgctgctcgc catcgcgcac ggcggcgtac agtcggctcc
     8521 acaagccttc gcccagggtt ttggtcacca ccagaccctg gcgctcaaaa ctctctacta
     8581 cccaatgcgg tagcgcggca atacccatcc gcgcggcaac catttgaatc aacaataagg
     8641 tgttatcgac gcttttcagt gacgggctga cgcctgccgg ctgaagaaaa tgccgccaga
     8701 catccagtcg actacgctgc accggataaa ttaatagcgt ctcgctggcg agatcttccg
     8761 gtgtaatacg cgtttttgcc gccagtggat ggtcaggtgc taacaccagg cgtacttcat
     8821 aatcgaacat cggcgaataa tgcaggccac tgcgcggcag gatatcggag gtcatcacca
     8881 gatccagctc gccctgttgc aacgcgggct gcgggtcaaa cgtcacgccc gatttaaaat
     8941 ccatctctac ctgcggccag ttcttatgga aatttcctaa agcgggtgtc agccactgaa
     9001 tacagctatg gcactcaatg gcaatgcgca gacgcgtctg ctgcggttca ttgcaggctt
     9061 gcagtgcctg gctgatttgc ggcagcacct ggtttgccag ttgcaacagg atttctccct
     9121 gcggtgtaaa gcgtagcggc tggctcttac gcacaaatag ccggaagcca aggcgttgtt
     9181 ccagatcgct aaactggtga gacagggcgg attgcgtctg atgcaacgtt gccgcagcgg
     9241 ctgcgagcga gccgcagttc cgcaacgctt gtagcgtttt caggtgtttt acttcgatca
     9301 tgaaagtcct tcacttcggc atgaataatt tgcgcttgag gaatatacag taaccgccaa
     9361 ttatggatgt gtaaacatct ggacggctaa aatcattcgt cttttaaatt tatggtgcgt
     9421 tggctgcgtt tctccacccc ggtcacttac ttcagtaagc tcccggcgat gaataaactt
     9481 gccgccttcc ctaaattcaa aatccatagg atttacatat aattagagga agaaaaatga
     9541 caatattgaa tcacaccctc ggtttccctc gcgttggcct gcgtcgcgag ctgaaaaaag
     9601 cgcaagaaag ttattgggcg gggaactcca cgcgtgaaga actgctgaca gtagggcgtg
     9661 aactgcgtgc tcgtcactgg gatcaacaaa agcaagcggg tatcgacctg ctgccggtgg
     9721 gcgattttgc ctggtacgat
//
LOCUS       DAZXUK010000145         9681 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZXUK010000145 DAZXUK010000000
VERSION     DAZXUK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.145"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(44..214)
                     /locus_tag="ACH3L8_002737"
                     /pseudo
     CDS             complement(44..214)
                     /locus_tag="ACH3L8_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACH3L8_002738"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACH3L8_002738"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFT4085789.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="ACH3L8_002739"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="ACH3L8_002739"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFT4085790.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="ACH3L8_002740"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="ACH3L8_002740"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFT4085791.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="ACH3L8_002741"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="ACH3L8_002741"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFT4085792.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="ACH3L8_002742"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="ACH3L8_002742"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFT4085793.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6242
                     /locus_tag="ACH3L8_002743"
     CDS             5109..6242
                     /locus_tag="ACH3L8_002743"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFT4085794.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6331..6864
                     /gene="blc"
                     /locus_tag="ACH3L8_002744"
     CDS             6331..6864
                     /gene="blc"
                     /locus_tag="ACH3L8_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFT4085795.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="ACH3L8_002745"
     CDS             complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="ACH3L8_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFT4085796.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="ACH3L8_002746"
     CDS             complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="ACH3L8_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFT4085797.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="ACH3L8_002747"
     CDS             complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="ACH3L8_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFT4085798.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7788..8354)
                     /gene="efp"
                     /locus_tag="ACH3L8_002748"
     CDS             complement(7788..8354)
                     /gene="efp"
                     /locus_tag="ACH3L8_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFT4085799.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8396..9424
                     /gene="epmB"
                     /locus_tag="ACH3L8_002749"
     CDS             8396..9424
                     /gene="epmB"
                     /locus_tag="ACH3L8_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFT4085800.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2480 c         2466 g         2270 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg
     5881 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5941 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     6001 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6061 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6121 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6181 aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt
     6241 aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac
     6301 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     6361 acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta
     6421 gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat
     6481 caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     6541 ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa
     6601 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     6661 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     6721 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     6781 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     6841 tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg
     6901 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg
     6961 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt
     7021 aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata
     7081 acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact
     7141 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaatttgggg
     7201 ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     7261 attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca
     7321 aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     7381 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7441 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7501 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7561 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7621 gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta
     7681 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7741 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7801 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7861 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7921 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7981 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     8041 ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8101 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8161 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8221 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8281 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8341 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8401 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8461 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8521 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8581 catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga
     8641 gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8701 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8761 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8821 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8881 ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact
     8941 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     9001 ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct
     9061 ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa
     9121 attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga
     9181 taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta
     9241 ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     9301 agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact
     9361 ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca
     9421 gtaactgatt aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg
     9481 cctacatcgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt
     9541 tacgccgcat ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct
     9601 ttgcacgttg cataaccttt aattattcat atgcattgct gttgttactt tttatatagc
     9661 taacaccaat aattaacctt t
//
LOCUS       DAZXUK010000146         9644 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZXUK010000146 DAZXUK010000000
VERSION     DAZXUK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.146"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            2..1369
                     /gene="dcuC"
                     /locus_tag="ACH3L8_002750"
     CDS             2..1369
                     /gene="dcuC"
                     /locus_tag="ACH3L8_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFT4085801.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMPFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACH3L8_002751"
     CDS             complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACH3L8_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFT4085802.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACH3L8_002752"
     CDS             complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACH3L8_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFT4085803.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACH3L8_002753"
     CDS             complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACH3L8_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFT4085804.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACH3L8_002754"
     CDS             complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACH3L8_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFT4085805.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACH3L8_002755"
     CDS             complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACH3L8_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFT4085806.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5324..5722
                     /gene="zapG"
                     /locus_tag="ACH3L8_002756"
     CDS             5324..5722
                     /gene="zapG"
                     /locus_tag="ACH3L8_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFT4085807.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            5876..7223
                     /gene="degQ"
                     /locus_tag="ACH3L8_002757"
                     /pseudo
     CDS             5876..7223
                     /gene="degQ"
                     /locus_tag="ACH3L8_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            7313..8380
                     /gene="degS"
                     /locus_tag="ACH3L8_002758"
     CDS             7313..8380
                     /gene="degS"
                     /locus_tag="ACH3L8_002758"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFT4085808.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACH3L8_002759"
     CDS             complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACH3L8_002759"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFT4085809.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
BASE COUNT         2338 a         2465 c         2444 g         2397 t
ORIGIN      
        1 aatgttcggc ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa
       61 aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga tgtgcggagt
      121 ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga
      181 catttataat gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat
      241 tatggcggtg ggcggttatg cccgctacat ggagcgcata ggagccagtt gcgcgatggt
      301 gagcttgcta agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac
      361 ttacgtcatc ggccaaatca tggcgcagtt tattaccagc gcctccggtc tgggtatgtt
      421 gctgatggtc accttatttc cgacgctggt gagtctggga gtaagtcgcc tctctgcggt
      481 ggcggttatc gcaaccacga tgtccattga gtgggggatt ctggaaacga actccatttt
      541 tgcagcccag gtcgcgggaa tgaaaattgc cacatacttc ttccactacc agcttccggt
      601 cgcctcttgc gtcattatct cggtggcgat ctcccacttt ttcgtgcaac gcgcttttga
      661 caaaaaagat aaaaatatca atcacgaaca ggcagagcaa aaaactctcg ataatgtccc
      721 gccgctctat tacgccattt tacctgtgat gccgttaatc ctgatgctcg gctcgctgtt
      781 cctcgcccac gtcgggctga tgcagtcaga actgcatctg gtggtggtga tgttactaag
      841 tttgacggtg acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga
      901 cgatgtgcag gcgttttttg acggcatggg tacgcagttt gtcaacgtgg taacgctggt
      961 ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt ggcactgttg atgcggttat
     1021 caggggtgcg gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctagt
     1081 cattgccatt tgtgccattg tgatgggctc tggcaatgcg ccgtttatgc catttgccag
     1141 tcttattccg aatatcgcag ccggactaca tgtaccagcg gttgtaatga ttatgccgat
     1201 gcattttgcc acgacgctag cgcgcgcggt ttcgccgatt actgcggtgg tggtcgttac
     1261 gtcaggaatt gcaggcgttt cgccttttgc ggtggtgaag cggacagcga tccccatggc
     1321 agtcggtttc gtggtgaata tgattgccac aatcacgcta ttttattaag tcattaaaaa
     1381 gacaaaacag gccgcctggg cctgttttgt attacttcac aacgcgtaat gccggtcgac
     1441 caccgcgtgg tggctgcgga ggttcatcgt caggatgagt gtcatcatcg tgatctggct
     1501 tgtcgccatc aataaccgac ataacggttt cgttgtctgc cgatgcctct tcatcattca
     1561 tgatgctggt atcttcatcg taggcagctt caggctcaaa catcgtgcct gcgccatttt
     1621 cacgggcgta gatagccagc acggcagcca gcggcacaga aacctgacgc ggaatgccac
     1681 caaagcgcgc gttaaagcgc acctcatcat tcgccagttc cagattgccg acagcacgcg
     1741 gcgcaatgtt gagtacgatt tgcccgtcac gcgcatattc cataggaacc tgcacgccag
     1801 ggagcgtcac atccaccacc aggtgcggcg tgagctggtt atccagcaac cactcataga
     1861 atgcacgcag cagataggga cgacgtggtg ttagctgtga caaatccata cagattaact
     1921 ccggcccaga cgcatttcac gttctgcttc agttaaagaa gcaaggaaag agtcacgctc
     1981 aaagacgcgg gtcatatagc ctttcagctc tttcgcaccc gggccgctga actcgatgcc
     2041 cagttgcggc agacgccaca gcagcggagc aaggtagcaa tcgaccaggc tgaactcatc
     2101 gctcaggaag tacggcttct gaccgaagac cggcgcaatc gccagcagtt cttcgcgcag
     2161 ttgcttacgt gcggcatctg cttcagaagc tgaaccgttg atgatagtgt tcatcagcgt
     2221 gtaccagtct ttttcgatgc gatgcatgta cagacggctt tcaccgcgag ctaccgggta
     2281 aacaggcatc agtggcggat gcgggaaacg ctcatccaga tattccataa tgatgcgaga
     2341 ttcccacagg gtcagctcac gatccaccag ggtcggaacg ctctgattcg ggttgaggtc
     2401 aatcagatcc tgaggcggat tgtccttttc cacgtgttcg atctcgaaac ttacaccttt
     2461 ctcagccagc acaatgcgga cctgatggct atagatgtca gtaggaccgg aaaacagcgt
     2521 cattaccgaa cgtttgttgg cagcgacagc catgaaaacc tccaggtata gtcagaattt
     2581 ttactgctac cagccaccag gtggccagtc agaagttgtg ttacccaata aggaacgact
     2641 ctctttgttc gaaaatcaaa caaaaaatga gcaatacccg acatttgggc agaaaattgg
     2701 atgatagttt accagatttt gcgacctttg tggtgagtgg atgccggaaa tggggaaaaa
     2761 gagatgcgct ttagtctgaa atagttgact tagaccctta ttggcgatgt ggtttttgtt
     2821 ttacctgtct ttcagacggc agcaaaaagc aacttcccat tttttacgct gattcagaat
     2881 ttagccataa aaaaacccgc cgaagcgggt tttttcgcaa attgttttct gccgtagcag
     2941 aagccaatta acgtttggag aactgcggac gacgacgtgc tttacgcaga ccgactttct
     3001 tacgttcaac ctgacgagcg tcacgagtaa cgaagccagc tttacgcagt tcagaacgca
     3061 gggactcgtc gtattccatc agagcgcggg tgataccgtg acggatcgca ccagcctgac
     3121 cagagatacc accaccttta acggtgatgt acaggtccag tttctcaacc atgtcgacca
     3181 gttccagcgg ctgacgaact accatgcggg cagtttcacg accgaagtac tgttccagag
     3241 aacgttggtt gattacgatt ttaccgttgc ccggtttgat gaaaacgcga gctgcggaac
     3301 ttttgcggcg accagtgccg tagtattgat tttcagccat tgcctataat cccgattaga
     3361 tgtcaagaac ttgcggttgc tgtgccgcgt ggttgtgctc gttacccgcg taaactttca
     3421 gtttacggaa catagcacga cccagcgggc cttttggcaa catgccttta accgcgattt
     3481 caatcacacg ctcaggacgg cgagcaatca tctcttcaaa ggtcgcttgt ttgataccac
     3541 cgatgtggcc ggtgtggtga tagtacactt tgtcagtacg cttgttgccg gttacagcaa
     3601 ctttgtcagc gttcagaacg atgatgtaat caccggtatc tacgtgcgga gtatattccg
     3661 ctttgtgctt accgcgcagg cgacgagcca gttcagtagc cagacggccc agagttttac
     3721 cggtcgcgtc aacaacatac cagtcgcgtt ttacggtttc tggtttagct gtaaaagttt
     3781 tcattaaaag cttacccaat aaatagttac acgttggtga acacccaaac gtcttcaatt
     3841 gttgaggttc acacgacaaa gtccggcaaa cctacccctt cgaatagcct atgccagcac
     3901 acaaaaagtt ttgggaaaaa aactttcttg taacgtgggg tcgcaggatt atagagaagt
     3961 cggggtcaaa gatcgacccc tttttgtgat ttgtggcagg ttttaacccg ccaaatgctc
     4021 gcgcttcaga tactcttcgc tttgcatctc ttgcagacgt gacaggcaac gctggaactc
     4081 aaacttcagc cgctcaccct gataaatttc atacagcggc acttctgcac tcaccactaa
     4141 tttgacatgg cgctcgtaaa actcatccac cagcgcaata aagcgccgcg cttcgctctc
     4201 catcaaccgc gtcatgactg gcacatcaaa caacattact gtgtgaaaca ggcgtgagag
     4261 cgcaatatag tcatgctgac tgcgggcgtc gacgcacagc gtagtaaaag aaaccgccag
     4321 cgtctggttc tcgacgccca ttgtcgccaa tggccgatgg ttgatttcta acgtcggtga
     4381 attttctcgt ttcacccccg ccagcgccaa ccatagttta tccatctgcg cccgggtttc
     4441 atcgttgagt ggcgaaagcc acagatgcgc ctgagtgagt gtacgcagtc gataatcaac
     4501 accagcgtcc acgttcatta catcacaatg ctgtttaatg gcatcgattg caggcagaaa
     4561 acgcgcacgt tgcaggccat ttcgataaag ttcgtccggc ggaatatttg acgtcgctac
     4621 cagggtaata ccgcgggtga acagggcttt catcagaccg ccaagtagca tggcgtcggt
     4681 aatatcagaa acaaaaaatt cgtcaaaaca gagcacgtca gtttcggctt taaagcgatc
     4741 ggcaataatt tccagcggat cggtctgccc ctgtaaggca gttagctctt cgtgcacccg
     4801 cagcataaaa cggtgaaagt gcaggcgctg tttccgctct cccggcaggc tttgatagaa
     4861 aaggtccatc agccaggttt tcccgcgtcc tacaccgccc cacatatata agccacgcac
     4921 tggcgtatgc tttgtgtctt cgcgtttacc ccacagctta ccgacccgcg ccattagccc
     4981 actcgtcctg ggggctggtg gcctgctatt gatgagttct tgataaataa tttccaggcg
     5041 gctgacggcc tctttttgaa cgtcgtcggg ttgatggctg ccttcattga gcgccttcag
     5101 gtattgcgat gttggggtaa cgctttgcat gatcttattg ttattccttg aataatcggt
     5161 gcgccgttgt tcacggttga cgaaaaaaag gccgttctac actacgcgat atgcagtcgg
     5221 gattccactt ctgtggaatt aacggttata gtggcataat cagccgcagg catggagcct
     5281 gaagccaaca ccctacggaa acaaaagaca acgggagatg ttcatgacct gggaatatgc
     5341 gctaattggg ttagtcgtcg gcatcattat tggtgctgtg gccatgcgtt ttggtaatcg
     5401 taaactacgc cagcaacagg cgttgcagta cgaactggaa aagaataaag ctgaactgga
     5461 cgagtatcgc gaagagctgg ttagccactt tgcccgcagc gcggaattac tggataccat
     5521 ggcgcacgac tatcgccagc tgtatcagca catggcaaaa agctccagca gcctgctgcc
     5581 ggaactgtct gctgaagcaa acccgttccg taatcgtctg gcagagtctg aagccagcaa
     5641 cgatcaggca ccggtgcagg tgcctcgcga ctattctgaa ggcgcatccg gcctgctgcg
     5701 tactggcgcg aagcgcgact aatttatttt tcgggcgcag ccattgcgcc ctcctcttct
     5761 ctccctcccc gactatcatt taatctggtg tctcattgtt agccgtctga aaattcaata
     5821 acatcaaact gttttgaatc tcttttctta tcattcaggt acgagagcag gaataatgaa
     5881 aaaacaaacc cagctgttga gtgcattagc gttaagtgcc gggttaactc tctcggcgtc
     5941 atttcaggcc gtcgcgtcga ttccaggcca ggttgccgat caggcccctc tccccagtct
     6001 ggctccaatg ctggaaaaag tgcttccggc agtggtgagc gtacgggtgg aaggaacggc
     6061 cagtcaggga cagaaaatcc cggaagaatt caaaaagttt tttggtgatg atttaccgga
     6121 tcaacctgca caacccttcg aaggtttagg ttccggtgtc atcatcaacg ccagtaaagg
     6181 ctatgtgctg accaacaacc atgtgattaa tcaggcacag aaaatcagta ttcagctcaa
     6241 tgatgggcgc gagtttgatg caaaactgat tggtagcgat gaccagagcg atatcgccct
     6301 gttataaatt caaaacccga gcaaattaac gcaaatcgct attgccgact ccgataaatt
     6361 gcgcgtcggt gattttgccg tagcggtcgg taacccattt ggccttgggc aaaccgccac
     6421 ctctggcatt gtttccgcat taggccgcag cgggttgaat cttgaaggtc tggaaaactt
     6481 tatccagaca gatgcttcca ttaaccgcgg taactccggc ggtgcactat taaaccttaa
     6541 cggtgagtta attggcatca acactgcaat ccttgcgcct ggcggcggga gcgtcgggat
     6601 tggatttgcc atccccagta atatggcgcg aacactggcg cagcagctta tcgactttgg
     6661 tgaaatcaaa cgcggtttgt taggcatcaa aggcaccgag atgagtgccg atatcgccaa
     6721 agccttcaac cttgacgtgc agcgtggcgc gtttgtcagc gaagtgttgc caagttctgg
     6781 ctcagcgaaa gcgggcgtca aagcgggcga tattattacc agcctcaacg gcaaaccgct
     6841 gaatagcttt gctgagttgc gctctcgtat cgcgactaca gagccgggca cgaaagtgaa
     6901 gcttggcctg ctgcgtaacg gcaaaccact ggaagtagaa gtgacgcttg ataccagcac
     6961 ctcttcgtcg gccagcgctg gaaggtgcaa cgttgagcga tggtcagcta aaagatggcg
     7021 gcaaaggtat taagatagat gaagttgtca aaggaagccc agctgctcag gctggcttgc
     7081 aaaaagacga tgtgatcatt ggcgtcaacc gcgatcgggt gaactcgatt gctgaaatgc
     7141 gtaaagtgct ggcggcaaaa ccggccatca tcgccctgca aattgtacgc ggcaatgaaa
     7201 gcatctatct gctgatgcgt taatgtcgta aaccgggcat caggctgacg tgtgatgtcc
     7261 ggttaactcg tggtatgctg ctgccgttcc cttttttaat gacgcctcca tcatgtttgt
     7321 gaagctctta cgttccgttg cgattggatt aattgtcggc gctattctgc tggttgccat
     7381 gccttcgctg cgcagcctta acccgctttc cactccgcaa tttgacagta ccgatgagac
     7441 gcctgccagc tataatctgg cggttcgccg cgccgcgcca gcggtggtta acgtttacaa
     7501 ccgtggtttg aacaccaact ctcacaacca gcttgagatc cgcaccctgg gatccggtgt
     7561 aatcatggat caacgcggtt atatcatcac caataaacac gtcatcaacg acgccgatca
     7621 gatcatcgtc gccttacagg atggacgtgt atttgaagca ttgctggtgg gatctgactc
     7681 tctaaccgat ctggcggtac ttaaaattaa tgccactggc ggtttaccta ccattccaat
     7741 taatgcacgt cgcgtaccgc acattggcga cgtagtactg gcgatcggta acccgtacaa
     7801 cctcgggcag accattaccc aagggattat tagtgccacg ggtcgaatcg gtctgaaccc
     7861 gaccgggcgg caaaacttcc tacaaaccga tgcttccatt aaccacggta actctggcgg
     7921 cgcgctggtg aactcgctgg gagaactgat gggcattaac acgctgtcgt ttgataagag
     7981 taacgatggc gaaacgccgg aaggtatcgg ctttgcgatt cctttccagt tagcaaccaa
     8041 aattatggat aagctgatcc gcgatggtcg cgtgatccgc ggctacattg gtatcggcgg
     8101 acgcgagatc gcaccactgc acgcgcagag cggtggtata gatcaactgc aagggatcgt
     8161 ggttaatgaa gtgtcacctg acggcccagc ggcgaatgcg ggtattcagg tcaacgatct
     8221 gattatttcg gtggataaca aaccggccat ctctgctctg gagacgatgg atcaggtggc
     8281 ggaaattcgc cctggttcgg tgatcccggt tgtagtgatg cgtgatgata agcagttaac
     8341 gctgcaggtc accattcagg aatatccggc aaccaattaa gtcgagtgct caaaacaaaa
     8401 accggagtct gtgctccggt tttttattat tcgctaatca attacttatt aacgaactct
     8461 ttgcccaggg cgatatcttt cttgagcgta tccagcatac cttccagcgc gttctgttca
     8521 aatgcgctca gggtaccgat agatttacgc tcttccacgc cgtttttacc cagcagcagc
     8581 ggttgagaga agaaacgggc gtactgaccg tcgccttcaa cgtaggcaca ttcgacaacg
     8641 ccttgttcgc cctgcagtgc acgaaccaga gacagaccaa aacgtgcagc tgcctggccc
     8701 atagacaggg ttgcagaccc gccaccggct ttcgcttcaa ccacctcagt acccgcgttc
     8761 tggatacgtt tggtcagatc agccacttcc tgctcggtaa aactaacgcc aggaacctgt
     8821 gacagcagcg gcagaatggt aacaccagag tgaccgccaa taaccggcac ttcaacttcg
     8881 cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg aacgaatgat atccagcgtg
     8941 gtaacgccga acagtttgtt tttgtcataa acaccggctt ttttcagcac ttcagcagca
     9001 attgcaactg tggtgttaac cgggttagtg ataataccaa tgcacgcttt cgggcagttt
     9061 ttcgcaactt gctgtaccag gtttttcacg atgccggcgt taacgttaaa caggtcggaa
     9121 cgatccatac ccggtttacg cgctacgcct gcagagataa gaacgacatc tgcgccttcc
     9181 agcgccggag tcgcatcttc accagaaaaa cctttgattt tcacagcagt agggatatgg
     9241 ctcagatcga cggccacacc gggagtcact ggagcgatat catacagaga gagttctgaa
     9301 cctgaaggca gttgggtttt taacagtagt gcaagcgcct ggccaatacc gccagcagcg
     9361 ccgaggactg cgactttcat cctaaactcc ttattatatt gataaactaa gatatgttgc
     9421 tccgctgccg cgaccttaat ccacaaaatt gccgtttaca atgaccacat ctcaagaatg
     9481 tgtagtcacg caagtttagc gtttatgcat ttaattgccg taatcaggaa cctaacttac
     9541 gtaattaaca gtcgcttatg cattagcgca acattccaac aggtggtgac aatataccct
     9601 accgttcagc caaaacaaca tcaatttgat aacaattaat ttac
//
LOCUS       DAZXUK010000147         9566 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZXUK010000147 DAZXUK010000000
VERSION     DAZXUK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.147"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..490
                     /gene="fecI"
                     /locus_tag="ACH3L8_002760"
     CDS             <1..490
                     /gene="fecI"
                     /locus_tag="ACH3L8_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFT4085810.1"
                     /translation="TFESLYGTHHGWLKSWLTRKLQSAFDADDIAQDTFLRVMVSETL
                     STIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPEERESQLETLQL
                     LDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKAVEHCLLFRLEY
                     GL"
     gene            487..1440
                     /gene="fecR"
                     /locus_tag="ACH3L8_002761"
     CDS             487..1440
                     /gene="fecR"
                     /locus_tag="ACH3L8_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFT4085811.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1527..3851
                     /gene="fecA"
                     /locus_tag="ACH3L8_002762"
     CDS             1527..3851
                     /gene="fecA"
                     /locus_tag="ACH3L8_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFT4085812.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            3896..4798
                     /gene="fecB"
                     /locus_tag="ACH3L8_002763"
     CDS             3896..4798
                     /gene="fecB"
                     /locus_tag="ACH3L8_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFT4085813.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPTWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            4795..5793
                     /gene="fecC"
                     /locus_tag="ACH3L8_002764"
     CDS             4795..5793
                     /gene="fecC"
                     /locus_tag="ACH3L8_002764"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFT4085814.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            5790..6746
                     /gene="fecD"
                     /locus_tag="ACH3L8_002765"
     CDS             5790..6746
                     /gene="fecD"
                     /locus_tag="ACH3L8_002765"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFT4085815.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            6747..7514
                     /gene="fecE"
                     /locus_tag="ACH3L8_002766"
     CDS             6747..7514
                     /gene="fecE"
                     /locus_tag="ACH3L8_002766"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFT4085816.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8072..8329)
                     /locus_tag="ACH3L8_002767"
     CDS             complement(8072..8329)
                     /locus_tag="ACH3L8_002767"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085817.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(8412..9566)
                     /locus_tag="ACH3L8_002768"
                     /pseudo
     CDS             complement(8412..9566)
                     /locus_tag="ACH3L8_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2033 a         2734 c         2665 g         2134 t
ORIGIN      
        1 aacgttcgag tcgctttatg gcacacatca cggctggttg aaaagctggc tgacgcgcaa
       61 actccagtct gcttttgatg cagatgacat tgcccaggac acttttttgc gggtaatggt
      121 cagcgaaacg ctctcgacga tccgcgatcc tcgctccttc ctctgcacta tcgccaaacg
      181 cgtgatggtg gacctgtttc gccgaaacgc gctggaaaaa gcgtatctgg agatgctggc
      241 gcttatgccg gaggggggag cgccttcacc tgaggaacgc gaaagccaac tcgagaccct
      301 acaactcctc gacagcatgc tggacgggct aaacggcaaa acacgtgaag cgtttctgct
      361 ttcgcaactg gatggtctga catacagcga gattgcgcac aaactcggtg tttccatcag
      421 ctccgtgaaa aaatacgtgg cgaaagccgt cgagcactgc ctgctgttcc gtctggagta
      481 tgggttatga atcctttgtt aaccgattcc cgccgtcagg cgctgcgttc agcttcccac
      541 tggtatgccg tgctaagcgg cgagcgcgtc agcccacaac aggaagcgcg ctggcaacag
      601 tggtatgaac aggatcagga taaccagtgg gcctggcagc aggttgaaaa cctgcgcaac
      661 cagcttggcg gtgtgcctgg cgacgttgcc agccgggcgt tgcacgatac ccgcctcacc
      721 cgccgtcacg tgatgaaagg attactgctg ttgctcggcg ctggcggagg ctggcagctc
      781 tggcagtcgg aaaccggcga aggtctgcgg gcagattacc gcaccgccaa aggcacagtc
      841 agccgtcagc aactggaaga tggctccctg ctcacgctga atacccaaag cgcggcggat
      901 gtgcgttttg atgcgcatca gcgcaccgtc cggctctggt acggtgaaat cgccattact
      961 accgcgaaag atgccctgca acgccccttc cgtgtcctga cccgtcaggg ccagctcact
     1021 gctttaggga cagaatttac cgtccgccag caggataatt tcacgcagct tgacgttcag
     1081 cagcacgctg tggaagtgct tctcgccagt gcccccgcgc aaaaacgcat cgtgaacgct
     1141 ggtgaaagcc tgcagttcag cgcctctgag tttggcgcag tgaaaccgct ggatgacgag
     1201 agtacaagct ggacgaagga catcctgagc ttcagcgata aaccgctggg tgaggtgata
     1261 gccacgctaa cccgttaccg caacggcgtg ctgcgctgcg atcccgccgt tgccgggctg
     1321 cgcctgagcg ggacgttccc gctgaaaaat accgatgcga tcctgaacgt tatcgcgcaa
     1381 acgcttcccg ttaaaattca gtctattacg cggtactgga taaacatttc accactgtaa
     1441 ggaaaataat tcttatttcg attgtccttt ttacccttct cgttcgactc atagctgaac
     1501 acaacaaaaa tgatgatggg gaaggtatga cgccgttacg cgtttttcgt aaaacaacac
     1561 ctttggttaa cgccattcgc ctgagcctgc tgccgctggc cggtctctcg ttttccgctt
     1621 ttgctgcaca ggttaatatc gcaccgggat cgctcgataa agcgctcaat cagtatgccg
     1681 cacacagcgg atttaccctc tcggttgacg ccagcctgac gcgcggcaag cagagcaacg
     1741 gcctgcacgg cgattacgac gtcgagagcg gcctgcaaca actgctggac ggcagcggac
     1801 tgcaggtaaa accgctggga aataacagct ggacgctgga gcccgcgccc gcaccaaaag
     1861 aagatgccct gaccgtggtc ggcgactggc tgggtgatgc gcgtgaaaac gacgtatttg
     1921 aacatgctgg cgcgcgtgac gtgatccgcc gtgaggattt cgccaaaacc ggcgcaacca
     1981 ccatgcgtga ggtacttaac cgcatccctg gcgtcagcgc gccggaaaac aacggcaccg
     2041 gcagccacga cctggcgatg aactttggca tccggggcct gaacccgcgc ctcgccagcc
     2101 gctcgaccgt cctgatggac ggcatccccg tccccttcgc cccttacggt cagccgcagc
     2161 tttcactggc tcccgtttcg ctcggcaaca tggatgccat tgacgtggta cgcggtggtg
     2221 gtgcggtgcg ttacggaccg cagagcgtgg gcggcgtggt gaactttgtt acccgtgcca
     2281 ttccgcagga ctttggtatc gaggcgggcg tggaaggtca gctcagccca acctcttcac
     2341 aaaacaaccc gaaagagacg cacaacctga tggtgggcgg cacagcggac aacggttttg
     2401 gcaccgcgct gctctactcc ggcacgcgcg gcagtgactg gcgcgagcac agcgccaccc
     2461 gcatcgacga cctgatgctg aaaagcaaat atgcgccgga tgaggtgcac accttcaaca
     2521 gcctgctgca atattacgac ggtgaagccg acatgcccgg tggcctgtct cgcgcggatt
     2581 acgacgccga tcgctggcaa tccacccgcc cgtatgaccg cttctggggt cgtcgcaagc
     2641 tggcgagcct gggctaccag ttccagccag acagccagca taaattcaac attcaggggt
     2701 tctacaccca aaccctgcgc agcggctacc tggagcaagg caaacgcatc accctctcgc
     2761 cgcgtaacta ctgggtgcgc ggtattgagc cacgctacag ccagatcttt atgatcggcc
     2821 cttccgcgca cgaagtgggc gtgggctatc gctatgtgaa tgaatcaacg catgaaatgc
     2881 gttactacac cgccaccagc agcgggcagt tgccgtccgg ctcaagccct tacgaccgcg
     2941 atacgcgttc cggcaccgag gcgcacgcct ggtatctgga tgacaaaatc gacatcggca
     3001 actggaccat cacgccgggt atgcgtttcg aacatatcga gtcataccag aacaacgcca
     3061 tcacaggcac gcacgaagaa gtgagctata acgcaccgct tccggcgttg aacgtgctct
     3121 atcacctgac tgacagctgg aatctttatg caaacactga aggctcgttc ggcaccgtac
     3181 agtacagcca gattggcaag gctgtgcaaa gcggcaatgt ggaaccggaa aaagcgcgaa
     3241 cctgggaact cggtacccgc tacgacgacg gcgcgctgac ggcggaaatg gggctgttcc
     3301 tgattaactt taacaatcag tacgactcca accagaccaa cgacaccgtc actgcacgtg
     3361 gcaaaacgcg ccataccggg ctggaaacgc aggcacgtta cgatctgggt acgctaacgc
     3421 caacgcttga taacgtttcc atctacgcca gctatgcgta tgtgaacgcg gaaatccgcg
     3481 agaaaggcga cacctacggc aatctggtac cattctcccc gaaacataaa ggcacgctgg
     3541 gcgtggacta caagccagga aactggacgt tcaatctgaa cagcgatttc cagtccagcc
     3601 agtttgcgga taacgccaat acggtgaaag agagcgccga cggcagtacc ggccgcattc
     3661 ccggcttcat gctctggggt gcacgcgtgg cgtatgactt tggcccgcag atggcagatc
     3721 tgaacctggc attcggtgtg aaaaacatct tcgaccagga ctacttcatc cgctcttatg
     3781 acgacaacaa caaaggcatc tatgcaggcc agccgcgcac gctgtatatg caggggtcgt
     3841 tgaagttctg atgtgttaac gcccggcttg ccgggctttt agctggaatg tgattatgtt
     3901 ggcatttatc cgttttcttt ttgcaggcct gctgctggtg atcagccacg cctttgccgc
     3961 catggttcag gacgaacacg gcacgtttac actcgaaaaa acgccacaac ggattgtggt
     4021 gctggaactc tcgttcgccg atgcgctggc cgccgtggac gtcagcccga tcggtattgc
     4081 cgacgataac gatgcaaaac gcatcctgcc cgaagtgcgt gcgcacctga aaccgtggca
     4141 gtccgtcgga acgcgcgcgc agccgagcct ggaagccatt gccgctctga aaccagacct
     4201 gatcattgcc gacagcagtc gccatgcggg ggtttacatc gccttgcagc aaatcgcgcc
     4261 ggtactgctg cttaagtccc gcaacgaaac ctacgctgaa aatttgcaat ctgcggctat
     4321 catcggcgaa atggtgggta aaaagcgaga gatgcaggca cgtctggaac aacataaaga
     4381 gaggatggcg cagtgggcca gccagcttcc caaagggaca cgcgtggcct ttggcacatc
     4441 acgcgaacag caattcaacc tgcatactca ggagacctgg accggcagcg tgctggcctc
     4501 tctggggctg aacgttcccg ctgcgatggc gggttcgtcc atgccgtcca tcggcctgga
     4561 gcaactgctg gcggtcaatc ctacctggct gctggttgcc cactatcgcg aagagagcat
     4621 tgttaaacgc tggcaacaag atccgctctg gcagatgtta accgccgcgc agaagcagca
     4681 ggttgcttcg gtcgacagta acacctgggc gcggatgcgc ggtatttttg ctgcagagcg
     4741 tattgccgct gacacggtaa aaatcttcca ccatcagccg cttaccgttg tgaaatgacc
     4801 gcgataaaac acccgatgct gctgtggggg cttcccgttg cagcacttat tattattttc
     4861 tggctgagtc tgttttgcta ctcggccatt cctgtttccg gagcagatgc aacccgcgcc
     4921 ctgctgcctg gacacacgcc aacgctacca gaagcgctgg tgcaaaacct tcgtttgcca
     4981 cgaagcctgg tcgccgttct gatcggcgca agcctggcgc tcgcgggcac gctgctgcaa
     5041 accctgaccc acaacccaat ggcctctcct tcactgctcg gcattaacag cggcgcggcg
     5101 ctggctatgg cgcttaccag cgcgctgagt ccgacgccga ttgcaggcta ttctctgtcg
     5161 ttcatcgcgg catgcggggg cggcgtgagc tggctgctgg tcatgaccgc aggaggcgga
     5221 tttcgtcata cccatgacag aaacaaactg atcctcgcgg gtatcgcgct gtcggccttt
     5281 tgtatgggcc ttacccgcat caccctgctg ctggccgaag atcatgctta cggcatcttt
     5341 tactggctgg caggcggagt gtcccacgcc cgctggcagg atgtctggca gctcttgccg
     5401 gtggtggtca ctgcagtccc tgtcgtgtta ctgctggcga atcaactgaa cctgctcaac
     5461 ctcagcgaca gcaccgccca tacgctggga gtgaacctga cgaggctacg tttggtcatc
     5521 aatatgttag tgctgcttct ggttggtgcg tgcgtcagcg tggcaggtcc ggtggcgttt
     5581 atcggtctgc tggtgccaca tctggcgcgc ttctgggcag gcttcgatca gcgcaacgta
     5641 ctgccagtga gcatgctgct gggggccacg ctgatgctgc tggcagatgt actcgcacgc
     5701 gcgctggcct tccccggaga tctgcccgca ggcgcagtgc tggcgctgat tggcagccct
     5761 tgctttgtct ggcttgtgag gaggcgagga tgaaaattgc gctggttatt ttcatcaccc
     5821 ttgccctggc gggctgtgcg ctgttatcac tccatatggg agtgatcccc gtgccgtggc
     5881 gcgcgctgct gaccgactgg caggccggac gcgagcatta ttatgtattg atggagtacc
     5941 gactgccgcg cttgctgctg gcactgtttg tcggtgcagc cctcgccgtg gcgggcgtgc
     6001 tgatacaggg gattgtgcgc aaccctctgg catcaccgga tattctcggc gttaaccatg
     6061 ccgccagcct ggcctctgtg ggggctctac ttcttatgcc gtcactgccc gtgatggtgc
     6121 tgccgctgct ggcctttgcg ggcggcatgg cggggttgat attactgaag atgctggcaa
     6181 agacccacca gccgatgaag ctggcgctca ccggcgtggc gctttctgca tgctgggcca
     6241 gcctgacgga ttatctgatg ctctcgcgcc cgcaggatgt gaacaacgcc ctgctgtggc
     6301 tgaccggcag cttatggggc cgtgactgga gctttgtgaa gattgccatc ccgctgatga
     6361 ttttatttct gccgctgagc ctgagttttt gccgcgatct cgacctcctt gcactcggcg
     6421 atgcgcgcgc caccacgctc ggtgtgtcgg tgccccatac ccgattctgg gctttgttac
     6481 tagctgtcgc catgacatct accggcgtgg ccgcctgcgg cccgattagc tttattggtc
     6541 tcgtggtgcc gcatatgatg cgtagcatca ccggtggacg tcaccgcaga ctgctgcctg
     6601 tttcagccct gacaggtgcg ttgctgttgg tggttgccga tctgctggcg agaattattc
     6661 atcccccact ggagctcccg gttggcgtgc tgaccgccat tatcggtgcg ccgtggtttg
     6721 tctggttgct tgtgagaatg cgataaatga ctttacgaac tgaaaatctg acggtcagtt
     6781 acgggacaga caaggtactt aacgacgttt cactctcact gccaacgggg aagatcaccg
     6841 ccctgatcgg ccctaacggt tgcgggaaat cgacgctgtt aaactgtttt tcgcggcttt
     6901 taatgccgca gtctggcacc gtatttctcg gcgataatcc cataaatatg ctctcatcac
     6961 gccagttggc ccgcaggctt tcgctgctgc ctcagcacca tttaacgcca gaggggatca
     7021 cagtccagga gctggtttcg tatggtcgta atccctggct gtcactctgg gggcgtctct
     7081 ccgctgaaga caatgcacga gttaatgtcg ccatgaacca gacccggatc aatcatcttg
     7141 ccgttcgtcg gttaaccgag ctttccggcg gtcagcgcca gcgcgcattt ctggcgatgg
     7201 tcctggccca gaatacgccc gttgtattac ttgatgagcc aaccacctat cttgatatca
     7261 atcaccaggt ggacctgatg cggttgatgg gcgaactccg gactcagggg aaaacggtgg
     7321 tcgctgtgct gcacgacctt aatcaggcta gccggtactg cgatcaactg gtggtaatgg
     7381 caaacggaca tgttatggcg caaggcacac cagaagaggt gatgacccca ggattgctga
     7441 gaacagtatt cagcgtggaa gcggaaatac accccgagcc ggtatctggc aggccgatgt
     7501 gcctaatgag gtagattgca caggccgtaa gaaccaaacc acgactgaat gaaactggac
     7561 tggcgccagc aagcctgttc agactggggc tgaacttttc cggactctga aagattacca
     7621 atactcatcg tccatccgct tgctttaggc tgacaggttc ataatcaacg caaaccagag
     7681 ctgtacaggc ttgggcgcgg ctttcaaacc agtcgtgatc acggcaatca attttgaact
     7741 ctgcttaacg gacatttctg tataaccctt acggcaacga aaaacgcgaa gttaaaattt
     7801 tagaaaccca aaaacgtgac atgactaagt ttagatttca gggggggaga tcaaaaaatt
     7861 tcgctctgtg ccagagcgga cattcacgga gctggttcat taccaatgag gttgggcttt
     7921 tgaggataaa tcaatgatca gacgccaacg taaatcaaaa gcaaccctgg aacggaattg
     7981 ctaatccagt ttctgaccat cgatttttct aaaaagtgtg cgtttgctac tacttaggta
     8041 ggtgcagctt tcttaatcac cggcagccac gttatacagg ccagttgatg gatcgattgt
     8101 tatcaatgat atcttcatga gtcggtgtct cacccagctt accgaagctg gcataaagtg
     8161 aaggcagacg ggcccgtcct tctccctttt tcgccagagg gaaagcgcga agcatggtgg
     8221 cagcctccca gtcacaacaa taggatggtg tgcacggctg ctgacgccat gattcagcga
     8281 tagagccgga aaatacgggg tcaaagccgg tatcattaac cagagtcatc gtgacctgta
     8341 ctgcggctgg atgatctcct gctactgcaa ctgctaggcg accgcggctc ccttcaggta
     8401 gtctgttgtg gtcaactaaa actggcctcc gcgttagagt ttttccagta tcggttttct
     8461 gattcgtttg gtggtaaccc accattatat tcgtgcggtc ttagtgcgct gtaatatcca
     8521 acgatatagt ccgttattgc gtgagctgca tcgctgaagc ttacatagcc cgtcgctggc
     8581 acccattagt tcttcagact cctgaagaag cgctccattg ggctgttatc ccagcagttt
     8641 ccacgccggc tcatactctg tctgatctgg tatcgccaca gtaactgccg gaactgcctg
     8701 ctcgtataat gactgccttg atcgcttgga acatcacccc gacgggctta ccacgggttt
     8761 cccatgccat ttccagtgct ttcatggtaa gcctgctgtc cggcgagaac gagatggccc
     8821 agcccactgg ttttcttgcg aacaggtcga gaacaacggc gaggtacgcc cagcgcttac
     8881 ccgtccagat ataggtcaca tcaccgcacc acacctgatt tggttccgtt acggcgaact
     8941 gtcgctcaag atgattcggg atagcaacgt gctcatgacc gccacgctta taccggtgag
     9001 tcggctgctg gcaactgacc agccccagct ctttcatgag tctgccagca agccagcgcc
     9061 ccatctggta gcctctctgg gttgccattg tggcgatgct tcttgctccg gcagagccgt
     9121 ggctgatgcc atgcagttca agtacctggc tgcgtaatac agcccgtctg ccgtctggtt
     9181 tttcaggacg gtttttccag tatttgtagc tgctgcgatg gaccccgaac acatggcaga
     9241 gagtggccac aggataacgc gccctgagtt tcccgattat cgaaaactgt tcagggagtc
     9301 tgacatcaag agcgcggtag cctttttaat atttcatttt ccatttcaat acgttgtagc
     9361 tttttcctga gctcacggat ttcaatttgt tccggggtaa tgggggaggc ttttggtgtt
     9421 tttccctgcc gctcatcacg taattgtttc acccatcgcg tcattgtgga aaggccgaca
     9481 tccatagcgc tggctgcatc tgccacggtg tatttctggt caacgaccag ttgagcggat
     9541 tcgcgtttaa actctgcgct gaaatt
//
LOCUS       DAZXUK010000148         9520 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZXUK010000148 DAZXUK010000000
VERSION     DAZXUK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9520
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.148"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..371
                     /locus_tag="ACH3L8_002769"
     CDS             <1..371
                     /locus_tag="ACH3L8_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFT4085818.1"
                     /translation="GLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEVA
                     LFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGVI
                     WLSDDKNNLEVNLTAWQQPS"
     gene            368..790
                     /gene="arfB"
                     /locus_tag="ACH3L8_002770"
     CDS             368..790
                     /gene="arfB"
                     /locus_tag="ACH3L8_002770"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFT4085819.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            804..1514
                     /gene="nlpE"
                     /locus_tag="ACH3L8_002771"
                     /gene_synonym="cutF"
     CDS             804..1514
                     /gene="nlpE"
                     /locus_tag="ACH3L8_002771"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFT4085820.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(1669..2493)
                     /locus_tag="ACH3L8_002772"
     CDS             complement(1669..2493)
                     /locus_tag="ACH3L8_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFT4085821.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(2546..4264)
                     /gene="proS"
                     /locus_tag="ACH3L8_002773"
     CDS             complement(2546..4264)
                     /gene="proS"
                     /locus_tag="ACH3L8_002773"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFT4085822.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(4375..5082)
                     /gene="tsaA"
                     /locus_tag="ACH3L8_002774"
     CDS             complement(4375..5082)
                     /gene="tsaA"
                     /locus_tag="ACH3L8_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFT4085823.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(5079..5483)
                     /gene="rcsF"
                     /locus_tag="ACH3L8_002775"
     CDS             complement(5079..5483)
                     /gene="rcsF"
                     /locus_tag="ACH3L8_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFT4085824.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(5601..6416)
                     /gene="metQ"
                     /locus_tag="ACH3L8_002776"
     CDS             complement(5601..6416)
                     /gene="metQ"
                     /locus_tag="ACH3L8_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFT4085825.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(6456..7109)
                     /locus_tag="ACH3L8_002777"
     CDS             complement(6456..7109)
                     /locus_tag="ACH3L8_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFT4085826.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(7102..8133)
                     /gene="metN"
                     /locus_tag="ACH3L8_002778"
     CDS             complement(7102..8133)
                     /gene="metN"
                     /locus_tag="ACH3L8_002778"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFT4085827.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            8321..8896
                     /gene="gmhB"
                     /locus_tag="ACH3L8_002779"
     CDS             8321..8896
                     /gene="gmhB"
                     /locus_tag="ACH3L8_002779"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFT4085828.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            9260..>9520
                     /locus_tag="ACH3L8_002780"
     rRNA            9260..>9520
                     /locus_tag="ACH3L8_002780"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2392 a         2388 c         2468 g         2272 t
ORIGIN      
        1 gtggtttgtg tgccgatgat gagccggaag cgtggctgcg taacgatcac ctgggcattg
       61 atttgtggat tgagctgggg ctgccggatg agcgacgaat taagaaagcc tgcacccagg
      121 ccgcagaagt ggcgctgttt acctataata gtcgggcggc gcaaatctgg tggcagcaaa
      181 atcagagcaa atgtgtgcag tttgccaatc tttccgtctg gtatctggac gatgaacaac
      241 tggcgaaagt aagcgccttt gccgatcgta ccatgacgct gcaggcaacg attcaggatg
      301 gtgttatctg gttatcggat gataagaata atctggaagt taacttaacc gcctggcaac
      361 aaccttcatg attgtgattt cccgacatgt tgctattccc gatggtgagc ttgagatcac
      421 cgccattcgt gcgcagggcg ctggcgggca gcatgttaat aagacctcaa cggctattca
      481 tctgcgtttt gacattcggg cgtccagcct gccagagtat tacaaagagc gtctgctcgc
      541 cgccagccat catttgatca gcagtgatgg cgtgattgtc attaaggcac aggaataccg
      601 cagtcaggaa ctgaaccgcg aagccgctct ggcccggctg gtggcagtga ttaaagattt
      661 aacaaaagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
      721 gctggcatcg aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
      781 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
      841 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
      901 cgcaggctgc cgaactgaaa ccgatgccgc aaagttggcg cggcgtgcta ccgtgtgccg
      961 attgcgaagg aatcgaaatc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     1021 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     1081 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     1141 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     1201 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     1261 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     1321 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     1381 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     1441 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     1501 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     1561 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     1621 taaggcgttt acgccgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     1681 cccaacaaag cgacccgcct tgatataaat cccaggcttc aggtgcccaa catactgtaa
     1741 ctgcgtttct ggcttaaacg cagagacatc accaatacgc atatgcatca gatcggcagg
     1801 gctaatccag cccgattgcg ccagtgtaag tggatgcccg gatttggcaa atgcatcagt
     1861 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cacacactgc ttaactgcgc
     1921 tttcgccagg tcgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     1981 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataaccgct
     2041 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     2101 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacaatctg
     2161 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agaaagattg caacgtgact
     2221 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     2281 cagcaaatcg ccgggtttga gatcaggtgc cgttatttct tttattgatt gttcggtgaa
     2341 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     2401 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     2461 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     2521 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     2581 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     2641 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     2701 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     2761 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     2821 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     2881 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     2941 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     3001 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     3061 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     3121 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     3181 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     3241 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     3301 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     3361 gatcaccgcg caccagcagc gcaaccagcg ggaagctact gccttcaacc gctttaacca
     3421 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     3481 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     3541 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     3601 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     3661 tagaaccggt gtcggcctgt acggcgcgga aatccagccc catgcggctg aagattttgc
     3721 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     3781 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     3841 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     3901 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     3961 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     4021 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgatcgca ccggcgttgt
     4081 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     4141 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     4201 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     4261 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     4321 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     4381 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     4441 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     4501 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     4561 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     4621 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     4681 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgcc
     4741 tttatggcaa acaacctctt tcagctctac cagcgacatg ccaattgggt tagggcggaa
     4801 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     4861 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     4921 gaatgcttcc aggccgcgaa cggcgtcggc ctggttgtag gaagctatga gatgcagttc
     4981 gccgttggcg ctttttacca gacctggctg gcgcggaacg gcgaattttt ctttataggg
     5041 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     5101 gcagagccga tacatacagc ctgacgatag cagcccggcg taccgctggt gacttcgcag
     5161 ctatgcagta atacagcatt ggctttcatt ttagaggcat tgatttgcat ctgcttacgt
     5221 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
     5281 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
     5341 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
     5401 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
     5461 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
     5521 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
     5581 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
     5641 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
     5701 tctttgttat cttcacgcgt tacgatcagg tttacgtatg gggagtcttt atcttcgaca
     5761 aagataccgt ctttcgccgg agtcaggcca atctggctgg cataggtggt attgataact
     5821 gccagagcga tttgcgcgtc gtccagagaa cgcggcagtt gcggtgcttc cagttcaaca
     5881 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
     5941 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
     6001 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
     6061 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
     6121 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
     6181 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
     6241 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
     6301 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
     6361 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
     6421 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
     6481 atgcggtcgc ctgcaaactg aattaaataa accagaatga ccagcaatac cagtaccgta
     6541 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
     6601 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
     6661 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacagac gatctgcatt
     6721 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
     6781 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
     6841 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
     6901 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
     6961 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
     7021 atcacaaacc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
     7081 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
     7141 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
     7201 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
     7261 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
     7321 cagacgcagc atcggcacgc agtcagtaaa tggctccgtt tgcagacgtt cctggtaatc
     7381 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
     7441 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
     7501 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
     7561 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
     7621 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
     7681 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
     7741 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
     7801 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
     7861 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
     7921 cgtggtcaat tcctggccat cgaccagcac gctaccctcg gttgggcgct ccagcaagtt
     7981 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
     8041 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
     8101 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
     8161 gtcgtagcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
     8221 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
     8281 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
     8341 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
     8401 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
     8461 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
     8521 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
     8581 tggtatctat tattgcccgc atcatccgca ggggagtgtt gaagagtttc gccaggtctg
     8641 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
     8701 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
     8761 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
     8821 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
     8881 aaaaccggcg caatgattaa aagatgaacg gttgaaataa aaatgcattt ttccgcttgt
     8941 cttcctgagc cgactcccta taatgcgctt ccatcgacac ggcggatgtg aatcacttca
     9001 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaat
     9061 aggaaagtgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
     9121 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
     9181 agacgataaa tgaataccaa gtctcaagag tgaacacgta attcattacg aagtttaatt
     9241 ctttgagcat caaactttta aattgaagag tttgatcatg gctcagattg aacgctggcg
     9301 gcaggcctaa cacatgcaag tcgaacggta acaggaagcg cttgctgctt cgctgacgag
     9361 tggcggacgg gtgagtaatg tctgggaaac tgcctgatgg agggggataa ctactggaaa
     9421 cggtagctaa taccgcataa cgtcgcaaga ccaaagaggg ggaccttcgg gcctcttgcc
     9481 atcggatgtg cccagatggg attagcttgt tggtggggta
//
LOCUS       DAZXUK010000149         9513 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZXUK010000149 DAZXUK010000000
VERSION     DAZXUK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9513
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.149"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..276
                     /locus_tag="ACH3L8_002781"
     CDS             <1..276
                     /locus_tag="ACH3L8_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFT4085829.1"
                     /translation="PAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIV
                     VMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            432..1967
                     /gene="gadC"
                     /locus_tag="ACH3L8_002782"
     CDS             432..1967
                     /gene="gadC"
                     /locus_tag="ACH3L8_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFT4085830.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2098..3417
                     /locus_tag="ACH3L8_002783"
     CDS             2098..3417
                     /locus_tag="ACH3L8_002783"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFT4085831.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3794..4864
                     /gene="dosC"
                     /locus_tag="ACH3L8_002784"
                     /pseudo
     CDS             3794..4864
                     /gene="dosC"
                     /locus_tag="ACH3L8_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            4864..5481
                     /locus_tag="ACH3L8_002785"
                     /pseudo
     CDS             4864..5481
                     /locus_tag="ACH3L8_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5739..6071
                     /gene="ddpX"
                     /locus_tag="ACH3L8_002786"
                     /pseudo
     CDS             5739..6071
                     /gene="ddpX"
                     /locus_tag="ACH3L8_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            6085..6981
                     /gene="ddpC"
                     /locus_tag="ACH3L8_002787"
     CDS             6085..6981
                     /gene="ddpC"
                     /locus_tag="ACH3L8_002787"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFT4085832.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6978..7952
                     /locus_tag="ACH3L8_002788"
     CDS             6978..7952
                     /locus_tag="ACH3L8_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFT4085833.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7945..8871
                     /locus_tag="ACH3L8_002789"
                     /pseudo
     CDS             7945..8871
                     /locus_tag="ACH3L8_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            complement(8927..9358)
                     /gene="osmC"
                     /locus_tag="ACH3L8_002790"
     CDS             complement(8927..9358)
                     /gene="osmC"
                     /locus_tag="ACH3L8_002790"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFT4085834.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2321 a         2331 c         2389 g         2472 t
ORIGIN      
        1 ccggcggttt gcttcaaact gaaagatggt gaagatccgg gatacaccct gtacgacctc
       61 tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc
      121 accgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga ctttgctgaa
      181 ctgttgctgg aagactacaa agcctccctg aaatatctca gcgatcaccc gaaactgcag
      241 ggtattgccc agcagaacag ctttaaacat acctgataac gtttaacggt aacggtgtcc
      301 cgaaacgaac ccgtttcggg ataatttcca aagtctgttc actggcatta gcaacggaaa
      361 atattgttct gaatacgctt cagaacaaaa caggtgcggt tccgacagga ataccgtttt
      421 agggggataa tatggctaca tcagtacaga caggtaaagc taagcagctc acattactcg
      481 gattctttgc cataacggca tcgatggtaa tggctgttta tgaataccct accttcgcaa
      541 catcgggctt ttcattagtc ttcttcctgc tattaggcgg gattttatgg tttattcccg
      601 tgggactttg tgctgcggaa atggccaccg tcgacggctg ggaagaaggt ggtgtcttcg
      661 cctgggtatc aaatactctg ggtccgagat ggggatttgc agcgatctca tttggctatc
      721 tgcaaatcgc cattggtttt attccgatgc tctatttcgt gttaggggca ctctcctaca
      781 tcctgaaatg gccagcgctg aatgaagacc ccattaccaa aactattgca gcactcatca
      841 ttctttgggc gctggcatta acgcagtttg gtggcacgaa atacacggcg cgaattgcta
      901 aagttggctt cttcgccggt atcctgttac ctgcatttat tttgatcgca ttagcggcta
      961 tttacctgca ctccggtgcc cccgttgcta tcgaaatgga ttcgaagacc ttcttccctg
     1021 acttctctaa agtgggcaca ctggttgtat ttgttgcctt cattttgagt tatatgggcg
     1081 tagaagcttc cgcaactcac gtcaatgaaa tgagcaaccc cgggcgcgac tatccactgg
     1141 ctatgttact gctgatggtg gcggcaatct gcttaagctc tgttggcggt ttgtctattg
     1201 cgatggtcat tccgggtaat gaaatcaacc tctccgcagg ggtaatgcaa acctttaccg
     1261 ttctgatgtc ccatgtggca ccggaaattg agtggacggt tcgcgtgatc tccgcactgc
     1321 tgttgctggg tgttctggcg gaaatcgcct cctggattgt tggtccttct cgcgggatgt
     1381 atgtcacagc gcagaaaaac ctgctgccag cggcattcgc taaaatgaac aaaaatggcg
     1441 taccggtaac gctggtcatt tcgcagctgg tgattacttc tatcgcgttg atcatcctca
     1501 ccaataccgg tggcggtaac aacatgtcct tcctgatcgc actggcgctg acggtggtga
     1561 tttatctgtg tgcttatttc atgctgttta ttggctacat tgtgttggtt cttaaacatc
     1621 ctgacttaaa acgcacattt aatatccctg gtggtaaagg ggtgaaactg gtcgtggcaa
     1681 ttgtcggtct gctgacttca attatggcgt ttattgtttc cttcctgccg ccggataaca
     1741 tccagggtga ttctaccgat atgtatgttg aattactggt tgttagtttc ctggtggtac
     1801 ttgccctgcc ctttattctc tatgctgttc atgatcgtaa aggcaaagcg aataccggcg
     1861 tcactctgga gccaatcaac agtcagaacg caccaaaagg tcacttcttc ctgcacccgc
     1921 gtgcacgttc accacactat attgtgatga atgacaagaa acactaacca tgtctgaatg
     1981 gttataagac aagggagcga taattcatcg ctcccttttt cgtgcttgct gtcgtattga
     2041 cttaaccggg ataagtacga gaatgagcgc acatctgttt accggaaacc agcacatatg
     2101 gatatctgct cccgaaacaa gaaattaacg attagaagac cagcgatact ggttgcattg
     2161 gcacttttac tgtgtagttg taaaagcacg cctccagagt ccatggtgac accaccagca
     2221 ggttcaaagc caccagccac gacgcaacaa tcgtcacaac cgatgcgtgg catctggctg
     2281 gccacggttt ctcgcctcga ctggccaccg gtttcctcgg ttaacattag taatcccacc
     2341 agccgagccc gtgtacaaca acaggcgatg atcgacaaac tggatcatct ccaacgtctc
     2401 ggcataaaca cggtcttttt ccaggtcaag ccggacggta ccgccctgtg gccatcgaaa
     2461 attttgccgt ggtccgatct tatgaccggt aagattggtg aaaatccggg ttacgatccg
     2521 ctgcaattca tgctcgacga agcccacaag cgtgggatga aagtacacgc ctggtttaac
     2581 ccctatcgcg tatcggttaa tacgaagccc ggtactatca gggaactgaa tagcactctg
     2641 tctcaacaac cggcgagcgt ctatgtgcaa caccgtgact ggatcagaac gtctggcgat
     2701 cgctttgtcc tcgacccggg catccctgag gttcaggact ggatcacatc aatagtcgct
     2761 gaagtggttt cccgctatcc ggtagatggc gtgcagtttg acgactattt ctatactgaa
     2821 tcaccgggtt cacggctaaa tgataacgaa acgtaccgta aatacggagg cgcatttgcg
     2881 tcaaaagcag actggcggcg caacaatact cagcagttaa ttgcaaaggt atcacacacc
     2941 attaaaagca ttaagccggg agtcgaattt ggcgttagcc cggcaggcgt gtggcgtaac
     3001 cgatcacacg atccgctcgg ttccgatacc cgaggcgcgg cagcctatga cgaatcctac
     3061 gcagacaccc gtcgatgggt ggaacaagga ttgctggatt acattgctcc ccaaatttac
     3121 tggccgttct cacggagtgc cgcgcgttat gacgtgttgg caaaatggtg ggcggatgtc
     3181 gttaaaccaa ccaggacccg cctgtatatc ggtatcgcct tctataaagt gggtgaacct
     3241 tcaaagatag agccagactg gatgattaac ggcggcgtac cggaactgaa aaagcagctc
     3301 gatcttaacg atgcggtacc agaaattagc ggcaccatct tgttccgtga ggactatctg
     3361 aataaaccgc agactcaaca agcggtcagc tatctgcaaa gtcgttgggg cagttaaact
     3421 accatgctta ctttgtaaca agccggtgct ttacccaccg gcttgttaca ccttgtgaaa
     3481 tatcccacta gtaatcatgc ttacataagt caaattaacc acgcaattaa ccacacttag
     3541 ttaaacagct ataaatccct aataaaaatg tatgaatatg aaaaaccata cgatattcgc
     3601 tgtcaccgaa aaataattac gatgggctat actcttctga agaacagtag cgtagattga
     3661 aactgtctta agtcgcttat ttcttttcag tatatcttca tatttcagga gaatatggtg
     3721 cagttagtcg atctcgcacg ttgcgtttcg ttttgattcc gtctgatttc ccagccttat
     3781 aagggtgtga attatggaga tgtattttaa aagaatgaaa gatgagtgga ccggacttgt
     3841 cgaacaggca gatcctctca ttcgtgctaa agccgcagaa attgcccttg cgcatgctca
     3901 ttatctgagt atcgagtttt atcgaattgt ccgcatcgac ccgcatgccg aagaattctt
     3961 gagtaatgaa caagttgagc ggcagttgaa aagtgcgatg gaacgctgga ttattaacgt
     4021 gctttctgcc caggttgacg atgtcgaaag gctaatacaa atccagcata ccgtcgcgga
     4081 agtgcatgcc cgcataggaa ttccggtaga aattgtcgag atggggtttc gggtgctgaa
     4141 aaaaatcctc tatccggtaa tcttctcttc ggattattcc gccgcagaaa aacttcaggt
     4201 ctaccatttc tcgattaaca gtattgatat cgcgatggaa gtgatgaccc gcgcgtttac
     4261 ctttagtgac agtagtgcct caaaggaaga tgaaaactat cgtatcttct cgttactgga
     4321 aaacgccgaa gaagaaaaag aacggcaaat agcctcaata ctttcatggg aaatagatat
     4381 tatctataaa gtcctgctgg attctgattt aggcagcagt ttgcctttaa gccaggctga
     4441 ttttggcctg tggtttaacc ataaaggtcg acattatttt agcggcattg ccgaagtggg
     4501 ccatatctcc cgtctgattc aggatttcga cggtattttc aatcaaacca tgcgtaacac
     4561 caggattttg aataacagaa gtttgcgggt gaaatttcta ttacagataa gaaataccgt
     4621 atcgcagatt attaccttgc tgcgtgaatt gtttgaagaa gtatcgcgcc acgaagtcgg
     4681 tatggatgta ctgacgaaat tacttaaccg ccgtttcctg ccgactatct tcaaacgcga
     4741 aattgcccat gccaaccgga ccggtacacc gctgtcagtg ctgattattg acgttgataa
     4801 attcaaagag atcaacgata cgtggggcca taacactggc gatgaaattc tgcgtaaagt
     4861 ctcattcctc tcgcagaaga gattggtgaa atcgaaaata ttgggcgctg ggtcatcgcg
     4921 gaagcttgcc gtcagttagc agaatggcgt agccagaata ttcatatccc ggcgttatcc
     4981 gtgaacttgt cggcgctgca ctttcgcagt aatcagctgc ctaatcaggt gtctgatgca
     5041 atgcaagcct ggggtattga cggccaccag ctgacggtgg aaatcacgga aagcatgatg
     5101 atggaacacg ataccgaaat ctttaagcgc attcagatcc tgcgtgatat gggcgtgggt
     5161 ttatcggtag atgattttgg cacgggcttt tccggattat cccgcttagt cagtcttccg
     5221 gtaacggaaa tcaaaattga caaaagcttt gtcgatcgtt gtctgacgga aaaacgcatc
     5281 ctggccttac ttgaagccat taccagcatt gggcaaagcc tcaatttaac cgtcgtggcg
     5341 gaaggggtcg aaaccaaaga gcaatttgag atgctacgta agatccactg tcgcgttatt
     5401 cagggatatt tcttttcccg cccccttccc gccgaagaaa ttccaggctg gatgagcagc
     5461 gtgttaccgc tgaaaatctg acaaattcct ctcgcccgca ctcgcgggtt ttcctttaag
     5521 gtgacactgt cacttaaata ctgtgatttc agctacgatt ccgggagatt ccttccttaa
     5581 ctttccctct tgcaccaaaa ctgcactacg ctgcacaaat gttgatcaaa agttaaaaaa
     5641 tataacttta agccagcatt ctggcgctta tcccggcatg gcatgagatc tgcataagcg
     5701 gaaagcgcag caatttttgt cttatacagg aaccgtttat gtcggatacc accgaactgg
     5761 ttgatttagc cgtgatcttc cctgatctgg agatcgaatt gaaatatgcc tgcgttgata
     5821 acatcacagg taaagctatt tatcagcaag cgcgttgtct gctacataaa gatgcgatta
     5881 ccgcgctggc gaaaagtatc agtatcgccc agctgtcagg cttacaactg gtgatttacg
     5941 atgcgtatcg cccacaacaa gcacaggcga tgttgtggca agcctgccca gacccgcaat
     6001 atgttgttga tgtgacggtc ggttctaatc acagccgtgg cacggcgctc gacctgacgc
     6061 ttcgtgatga cgtggaggtg gtgaatgatg ctaagcgagg aaacgtccgc cgtacgccca
     6121 caaaaacaaa cgcgatttaa cggtgcaaaa ctggtatgga tgctgaaagg cagtccgctc
     6181 accgtgaccg gcgcagtcat cattgtatta atgcgactga tgatgatttt ttcaccgtgg
     6241 ctggcgacgc atgatcccaa cgccattgat ttaaccgccc gccttttgcc gccttctgcg
     6301 gcgcactggt ttggcaccga tgaagtggga cgcgatctgt ttagccgcgt actggtcggc
     6361 agtcagcaat caattctcgc cggattagtg gtggtcgcca ttgcgggtat gattggttcg
     6421 ctactcggat gtctatccgg tgtgcttggc ggacgcgcag acgccatcat catgcgcatc
     6481 atggacatta tgctgtcgat tccttcgttg gtactgacaa tggcactggc tgccgctctc
     6541 gggccgagtt tatttaacgc catgctggcg attgctattg tgcgaattcc cttttatgtg
     6601 cgcttggcgc gggggcaagc attagttgta cgccagtata cctatgttca ggcggcgaaa
     6661 acctttggcg cgtctcgttg gcatctgatc aactggcata ttttacgtaa ctccctaccg
     6721 ccgctgatcg tgcaggcatc gctggatatc ggtagcgcga ttttaatggc cgccacgttg
     6781 ggatttattg gcctgggtgc tcaacaaccg agtgctgaat ggggcgcgat ggtagcgaat
     6841 ggtcgcaact atgtactcga tcagtggtgg tattgcgcat ttccgggggc agcgattttg
     6901 cttacctccg tcgggtttaa tctctttggt gatggtattc gcgatctgct tgacccgaaa
     6961 gcaggaggaa agcagtcatg acccaacccg ttctggacat tcaacagctg catttgagtt
     7021 tccccggttt taacggtgat gttcacgcgc tcaacaatgt gtccttgcag attaaccgcg
     7081 gcgaaattgt cggtctggtg ggagaatccg gctcaggtaa atcagtcacc gcaatgctga
     7141 ttatgcgtct gctaccgacg ggcagttatt gcgtacatcg ggggcagatt tcactgctgg
     7201 gagaagatgt tcttaacgct cgggaaaaac agcttcgtca gtggcgcggc gcacgagtgg
     7261 cgatgatctt tcaggaaccg atgaccgccc ttaatccgac acgtcgaata ggtcttcaga
     7321 tgatggacgt aatccgccat catcaaccaa taagtcgtcg ggaagccaga gctaaagcga
     7381 ttgcactgct ggaagagatg caaatcccgg atgccgtgga agttatgtcg cgctatccgt
     7441 ttgagctttc aggtggtatg cgccagcggg taatgattgc gccggcattc tcctgcgagc
     7501 cgcaattgat tattgccggc gaaccgacga cggcgctgga cgtcacagta cagttgcagg
     7561 tactgcgttt gcttaaacat aaagcacgcg ccagtggaac tgcggtactg ttcatcagcc
     7621 atgatatggc cgtggtgtcg caactgtgcg atagcgttta cgtgatgtat gccggaagcg
     7681 taattgaaag cggcgggacg gcagacgtta tccatcatcc ccggcatccg tataccattg
     7741 gtttgctgca atgcgcacca gaacatggag taccacgcca gccattaccc gccattccag
     7801 ggacggtacc aaacctcacc catttgcctg acggctgcgc ttttcgcgat cgttgctatg
     7861 cggcaggtgc acagtgtgcg ctgacagcgt gtggtgacaa caaccagcac tgcacctgtt
     7921 ggtatccgca gcaggaggtc ataagtgtct gacacgttat taacgttacg cgacgtccat
     7981 atcaattttc cggcccgtaa aaactggctt ggcaaatcta cggaacatgt tcatgccatt
     8041 aatggtattg atttacagat ccgccgtggt gaaaccttag ggatcgtcgg cgagtcaggc
     8101 tgcggcaaaa gcaccctcgc acagctttta atgggcatgc tgcaaccgag ccacgggcag
     8161 tacatccgtt caggctcaca acgcattatg cagatggtgt ttcaggaccc gctctcttcg
     8221 cttaatccgc gcttaccggt gtggcgcatc atcacagaat cgctctggat agctaagcgt
     8281 agtagtgaac aacagcggcg agcgttggca gaggagttgg ctgtgcaggt gggtattcgt
     8341 ccggagtatc tcgaccgcct gcctcatgcg ttctccggcg ggcagcggca acgcatcgcc
     8401 attgccagag cactctcttc gcagcctgac gtgattgtgc ttgatgagcc aacctcggcg
     8461 ctggatatct ccgtgcaggc gcagatcctc aatttgctgg taacgctaca ggaaaatcac
     8521 gggctgactt atgtgctgat ttcacacaat gtctcggtga tacgtcatat gagcgatcgg
     8581 gtggcggtga tgtatctcgg gcagattgta gaactgggcg acgcgcaaca ggtgttgact
     8641 gcttcggcac atccatacac ccgattattg ctggattccc tccccgccat tgataaaccg
     8701 ctggaggaag aatgagcatt acgtaaaacg gatctgccgg gaaaccgcac gttgccgcaa
     8761 ggctgttttt tctacgaacg ttgccctctg gcaacccacg gatgtgaagt ccggcaatca
     8821 ttagcaataa gggaggacgg acgtgacctc cggtgctggc gggcgctgta gagatgctaa
     8881 caacgcatca ggcattcacg cgcctgacgc gtcatccggc aatgctttac gatttcaact
     8941 ggtaatccag cgtaatttcc gctttcagta cctgagagac cgggcatcct gcttttgctt
     9001 tctggatgat gccgtcaaag gtagaggcat caatacccgg caccgcaact tcactcttca
     9061 gtgcgatttt cgtaatcgca aaaccggcat ccactttatc cagcgacaca tcggcggtgg
     9121 tatcaattga tgttggcgtg aatcccgctt cccccagcat taatgaaagc gccattgaga
     9181 aacatgcggc atgcgctgcg ccaatcagtt cttcagggtt ggttcctttt tcgccttcaa
     9241 aacgcgtgtt aaatccatac ggctgttggt tcagcacgcc gctctcggtg gatactgttc
     9301 ccttcccgcg tttgatatcg ccttcccagt gtgcctgacc tttcttatgg attgtcattg
     9361 ttgctctcct gtgggcttaa aaaggaaatt atagactacg tgagaaacag cacgaggata
     9421 agcaggatat tccgaataaa atacggcgaa aatattactt ctgcacttaa attaaattcc
     9481 cacaatagat aaccgggaat atatatctca aga
//
LOCUS       DAZXUK010000150         9389 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZXUK010000150 DAZXUK010000000
VERSION     DAZXUK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9389
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.150"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1272
                     /gene="hpaX"
                     /locus_tag="ACH3L8_002791"
     CDS             <1..1272
                     /gene="hpaX"
                     /locus_tag="ACH3L8_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFT4085835.1"
                     /translation="IIFLFILFVFSFLDRINIGFAGLTMGKDLGLTSTMFGLAATLFY
                     VTYVMCGIPSNMMLAKIGARRWIAGIMVVWGIASTCTMFATSPETLYLLRMLVGIAEA
                     GFLPGILVYLTWWFPAYHRARANALFMIAMPVTMMLGLILSGYILALDGLWNLKGWQW
                     LFLLEGLPSVILGVVTWFYLNDTPDQATWLDENEKQALKTMILREQESAFAHASVPRS
                     TLREVLTPAIMLYTLAYFCLTNTLSAINIWTPQILQSFNTGSSNIMIGLLAAIPQFCT
                     IFGMIWWSRRSDRLKERKKHTILPYLFAAAGWLLASATDHSLIQLSSIIMASMGSFTA
                     MTIFWTTPDQVISLQSRAVAMAVINAVGNVGSAVSPLLIGILKDATGSFSSGLWFVAG
                     LLVLGALVMTRIPMGSREHSLSSELIAPKPQ"
     gene            1282..2172
                     /gene="hpaA"
                     /locus_tag="ACH3L8_002792"
     CDS             1282..2172
                     /gene="hpaA"
                     /locus_tag="ACH3L8_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFT4085836.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            2199..3136
                     /locus_tag="ACH3L8_002793"
                     /pseudo
     CDS             2199..3136
                     /locus_tag="ACH3L8_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            3363..4921
                     /gene="hpaB"
                     /locus_tag="ACH3L8_002794"
                     /pseudo
     CDS             3363..4921
                     /gene="hpaB"
                     /locus_tag="ACH3L8_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            4939..5451
                     /locus_tag="ACH3L8_002795"
     CDS             4939..5451
                     /locus_tag="ACH3L8_002795"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFT4085837.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            5844..7994
                     /gene="btsT"
                     /locus_tag="ACH3L8_002796"
     CDS             5844..7994
                     /gene="btsT"
                     /locus_tag="ACH3L8_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFT4085838.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            8044..8247
                     /locus_tag="ACH3L8_002797"
     CDS             8044..8247
                     /locus_tag="ACH3L8_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFT4085839.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            8258..9214
                     /gene="yjiA"
                     /locus_tag="ACH3L8_002798"
     CDS             8258..9214
                     /gene="yjiA"
                     /locus_tag="ACH3L8_002798"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFT4085840.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(9283..>9389)
                     /locus_tag="ACH3L8_002799"
     CDS             complement(9283..>9389)
                     /locus_tag="ACH3L8_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFT4085841.1"
                     /translation="YLTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         2235 a         2427 c         2387 g         2340 t
ORIGIN      
        1 atcatttttc tttttattct gtttgttttt tcgtttcttg atcgcatcaa tataggattt
       61 gccggactga caatgggcaa ggatcttggc ctgacgtcca caatgtttgg cctcgccgca
      121 acactctttt atgtcacgta cgtcatgtgc gggatcccca gtaatatgat gttagcgaaa
      181 attggcgctc gtcgatggat cgctgggatc atggtggtct ggggaattgc ttccacctgc
      241 acaatgtttg caacaagccc cgaaacactc tatctactgc gtatgctggt tggtattgct
      301 gaggcgggtt ttttacctgg tattttggtt tatctgacct ggtggttccc ggcatatcat
      361 cgggccagag ccaatgcctt atttatgata gcgatgccag tcactatgat gctgggattg
      421 atcctttcag gctacattct ggccttggat ggactgtgga atctaaaagg ctggcaatgg
      481 ctctttttgc tggaaggtct gccatcggtc attcttggtg tcgtcacctg gttttatctc
      541 aacgacacac cagaccaggc gacctggctt gatgaaaacg aaaaacaggc tttaaaaacg
      601 atgattctcc gtgaacaaga gagtgcattt gcccatgcat ctgttccacg ctcaacctta
      661 cgtgaagtcc tgaccccagc catcatgcta tatacacttg cttatttttg cctgactaat
      721 actctgagtg cgattaatat ctggacgccg caaatcctac agagtttcaa tactggcagt
      781 agcaacatca tgattggttt gctggcggcc atcccccaat tctgcacaat ttttggcatg
      841 atctggtgga gtcgtcgttc cgaccgtctt aaagaacgca aaaagcatac gattcttccc
      901 tacctgtttg ccgctgccgg atggttactc gcttctgcga cagatcacag cctcattcaa
      961 ctgagcagca tcattatggc ctcaatggga tcatttaccg cgatgactat cttttggaca
     1021 acaccggatc aagttataag cttgcaatcg cgtgccgttg cgatggctgt catcaatgct
     1081 gtcggcaatg taggttcagc agtcagtcca ttacttatcg gtattttgaa agatgcaacc
     1141 ggcagtttta gctcaggttt gtggtttgta gcaggattgc tggtactggg cgcgttagtc
     1201 atgacccgaa tccctatggg gagtcgggaa cactctcttt catctgaact tatcgcacca
     1261 aaaccgcaat aaggagtcat gatgtccagt gaagctatct ttgaacatgg ttatgaaaat
     1321 attgatttgg tccgggaata tgatgcccgc tacgctggtg aagatgtgca ttatgaaaca
     1381 tttgcccggt tagcgaattt ctttggacgc gatatgcgcc ctcactggca cgatagatat
     1441 tttcaactcc attatctggt cactggacgc attactttac aactggatga gcacttctat
     1501 tctgtgcgcg caccgctttt tgtactaaca ccaccatcgg tgccacatac tttctttact
     1561 gatcctgata gcgatgggca tgttctgacc gtacgtcagg agttaatttg gcccctggta
     1621 gaaaagctct ggcctggtag tggagatgcc gcaatgctac aaggtatctg tctgtcactg
     1681 gagggaatgg aacatacact ggcggcatta aatcattatt ggccgataat atccctggag
     1741 tttcaacaaa ataagcaagg acgggaaatt ctattgcaat cactggcgca aagtattttt
     1801 acaatcctac tgcgtgaagc accaccaaat gatatttcaa catgcagcgt gcgcggcgag
     1861 atgtgtttat tccagaagtt taatcggctg attgacgaaa attatcgcca acatttaatg
     1921 gtccctgaat atgcaacaat gctcggtatg agtgaatcac ggcttaccga gctgtgccga
     1981 cgttttgcca atcaatcgcc gaagagattg attttcgaac gagtgagccg ggaagccaga
     2041 cggcttctgc tctatagcga gcaaagcatc aaccagatag cccttgatct tggctacaaa
     2101 gatcccgcct atttcgcccg ttttttcaac cgaatgatgg gttgctcacc aacacaattc
     2161 cgtggtcgct gaccatttat caacctgaga gactcgacat gaaaatatct ttacgctcac
     2221 tatttgtggc gtgcctgatt gcgccagtgg cattcaccta cgcctccgaa ccgcaataaa
     2281 tgaaaattgt actacttgga acgaaaggtg gcccttcttt attaaaagct gaccgtttac
     2341 cgatggcaac ggcacttatt attggcaata aaacctggtt aattgatgct ggttacgggg
     2401 ccagcttaca actggtaaaa aataatattc cactgcgcca gattgataat attttactca
     2461 cccatttaca cagtgatcat attctggact atccatcagt cttaatgaat gcatgggcta
     2521 gcggtctgca ggacaaaact attcatgtat tcggtccgcc aggcacccga gccatgaccg
     2581 ctgccagttg gcaggtcttc gatcgggaca tcacactgcg tatggaagaa gaaggaaaac
     2641 ccgatcctcg caaactcgtt acagctcaag atgttggtga aggcgtagtt tataaagatg
     2701 ataccgtgac cgtgactgca ttaaaggtcc ctcattctcc tttccctgac ggtgaggctt
     2761 ttgcttatcg cttcgacaca caaggcaagc gaattgtctt ctcaggagat acctcgtact
     2821 ttcccccact ggcaactttc tcagaaggcg ctgatatact tgtgcatgag gccgtacatg
     2881 agccctccgt cgctaagctg gcagacagta ttggcaatgg taaaacactg gcaaaagcca
     2941 ttgcttctca ccatacgact attgaggatg taggcaaaat tgccaatgaa gcaaaagtga
     3001 aaaaactcat ccttagccac ctggtaccaa tcaccgttcc ggatgaaaca tggaagcaag
     3061 aagcacagaa aactattcag gtcctatcgt cgttggtcat gaaaatatga cgattaccat
     3121 tccattcgcc ccataaaaaa ttccgtcccc gttagccctt gttagcgggg aatattatga
     3181 tttgccttac tgtaagttga tcacgatcgc agtttactat ttaactccac cttgctgtcg
     3241 aaaagtacca acgttcgcct gaaacgtctc ttcccgacaa ccgcgttcaa cgcttcaatt
     3301 aaataacaaa aacaacacac aaaattaaca atcgtattcc gattaatact gtagaggtcg
     3361 acatgaaacc agaagatttc cgcgccagta cccaacgtcc tttcaccggg gaagagtatc
     3421 tgaaaagcct gcaggatggt cgcgagatct atatctatgg cgagcgagtg aaagacgtca
     3481 ccactcatcc ggcatttcgt aatgcggcag cgtctgttgc ccaactgtac gacgcgctgc
     3541 acaaaccgga gatgcaggac tctctgtgtt ggaacaccga caccggcagc ggcggctata
     3601 cccataaatt cttccgcgtg gcgaaaagtg ccgacgacct gcgccagcaa cgcgacgcca
     3661 tcgctgaatg gtcacgcctg agctatggct ggatgggccg taccccagac tacaaagccg
     3721 ctttcggttg cgcactgggc gcgaatccgg gcttttacgg tcagttcgag cagaacgccc
     3781 gtaactggta tacccgtatt caggaaactg gcctctactt taaccacgcg attgttaacc
     3841 caccgatcgt catttgccga ccgataaagt gaaagacgtt tacatcaagc tggaaaaaga
     3901 gactgacgcc gggattatcg tcagcggtgc gaaagtggtt gccaccaact cggcgctgac
     3961 tcactacaac atgattggct tcggctcggc acaagtgatg ggcgaaaacc cggacttcgc
     4021 actgatgttc gttgcgccaa tggatgccga tggcgtgaaa ttaatctccc gcgcctctta
     4081 tgagatggtc gcgggtgcta ccggctcgcc atacgactac ccgctctcca gccgcttcga
     4141 tgagaacgat gcgattctgg tgatggataa cgtactgatc ccatgggaaa acgtgctgat
     4201 ctaccgcgat tttgatcgct gccgtcgctg gacgatggaa ggcggtttcg cccgtatgta
     4261 tccgctgcaa gcctgtgtgc gcctggcagt gaaactcgac ttcattacgg cactgctgaa
     4321 aaaatcactc gaatgtaccg gcaccctgga gttccgtggt gtgcaggccg atctcggtga
     4381 agtggtggcg tggcgcaaca ccttctgggc attgagtgac tcgatgtgtt cagaagcgac
     4441 gccgtgggtc aacggggctt atttaccgga tcatgccgca ctgcaaacct atcgcgtact
     4501 ggcaccaatg gcctacgcga agatcaaaaa cattatagaa cgcaacgtta ccagtggcct
     4561 gatctacctc ccttccagtg cccgtgacct gaacaatccg cagatcgacc agtatctggc
     4621 gaagtatgtg cgcggttcga acggtatgga tcacgtccag cgcatcaaga tcctcaaact
     4681 gatgtgggac gccattggca gcgaatttgg tggtcgtcac gagctgtatg aaatcaacta
     4741 ctccggtagc caggatgaga ttcgcctgca atgtctgcgc caggcacaaa gctccggcaa
     4801 tatggacaag atgatggcga tggttgatcg ctgcctgtcg gaatacgacc agaacggctg
     4861 gactgtgccg cacctgcaca acaacgacga tatcaacatg ctggataagc tgctgaaata
     4921 acgcagcagg aggttaagat gcaattagat gaacaacgcc tgcgctttca tgacgcgatg
     4981 gccagcctgt cggcagcggt aaatattatc accaccgagg gcgacgccgg acaatgcggg
     5041 attacggcaa cggctgtctg ctcggtcacg gatacaccac cgtcgctgat ggtgtgcatt
     5101 aacgccaaca gtgcgatgaa cccggttttt cagggcaacg gcaagttgtg cgtcaacgtc
     5161 ctcaaccatg agcaggaact gatggcgcgc cacttcgcgg gcatgacagg catggcgatg
     5221 gaagagcgtt ttagcctctc atgctggcaa aaaggtccgc tggcgcagcc ggtgctaaaa
     5281 ggttcgctgg ccagtcttga aggagagatc cgcgatgtgc aggcgattgg cacacatctg
     5341 gtgtatctgg tggagattaa aaacatcatc ctcagtgcag aaggtcacgg acttatttac
     5401 tttaaacgcc gtttccatcc ggtgatgctg gaaatggaag ctgcgattta agctccagcc
     5461 ccttcacgca ttcgtcgaag gggcattttt attgtgatct caatcacata tctacccgat
     5521 gaataattct tgcccaaccg ctcaccgcca ttcctgaccg cttacatccc taaaataacc
     5581 actcagttat ttaccttact ttacgcgcac gtatctctgg caacatcact gcaggatagc
     5641 ggtcaattta cctcctcaaa cgcaacgcaa acctagaacg gcttcggcct atgattaaca
     5701 aattactcta tggattttgg tttgcaggaa ggcagcaagt gagtgaatcc ccgggagctt
     5761 acaaaagtaa gtgtcagagg tgaacgaacg cagctgccgc acctgtaagc caaaagacga
     5821 cgagtaaaat gccaggtttt actatggata cgaaaaaact attcaagcac ataccctggg
     5881 tgattctcgg aatcatcggt gcattctgcc tcgcggtagt tgcattacgt cggggggagc
     5941 acgtcagcgc cctgtggatc gtggtcgcct ctgtgtcggt gtatctggtg gcgtatcgct
     6001 actacagtct gtacatcgcc cagaaggtga tgaaactcga ccccacgcgc gcgacgcctg
     6061 cggttattaa caacgacggt ctgaactacg ttccgaccaa ccgttacgtg ttgtttggtc
     6121 accacttcgc cgctatcgcc ggtgctggtc cgctggtggg accggttctc gccgcgcaga
     6181 tgggctacct gcctggcacg ctgtggctgc tggcgggggt agtgctggcc ggtgcggtac
     6241 aggactttat ggtgctgttt atctcctctc gccgtaacgg cgcatctctc ggtgagatga
     6301 tcaaagaaga gatggggcca gtaccgggga ctatcgcgct gtttggctgt ttcttaatca
     6361 tgatcatcat cctcgccgtt ctggcgctga ttgtggttaa agccctggcc gaaagtccgt
     6421 ggggtgtctt caccgtttgc tcaaccgtac cgattgcgct gtttatgggt atctacatgc
     6481 gctttatccg tccggggcgt gtgggtgaag tctctgttat tggtatcgtg ctgctggttg
     6541 cctctatcta cttcggtggc gtgattgctc acgatccgta ctggggtccg gcactgacct
     6601 ttaaagacac caccattacc ttcgcgctga ttggctatgc gtttgtttcc gcactgctgc
     6661 cggtgtggct gatcctcgca ccgcgcgact atctggcaac cttcctgaaa atcggcgtta
     6721 tcgtcggcct ggcgctgggt atcgtggtgc tgaacccgga actgaaaatg cctgcgatga
     6781 cccagtacat tgacggtact ggcccgctgt ggaaaggcgc tctgttcccg ttcctgttca
     6841 tcaccatcgc ctgtggtgcg gtatctggct tccacgcgct gatctcttcc ggtacgacgc
     6901 cgaaactgct ggctaacgaa accgacgcgc gtttcattgg ctacggcgca atgctgatgg
     6961 agtccttcgt ggcgattatg gcgctggttg cagcgtccat catcgaaccg ggtctttact
     7021 tcgcgatgaa caccccgcct gctggccttg gcatcaccat gcctaacctg catgaaatgg
     7081 gtggcgagaa cgcgccgatc atcatggcgc agctgaaaga cgttaccgca cacgcggcag
     7141 cgaccgtcag ctcctggggc ttcgtgattt cgccagagca gatcctgcaa accgcgaaag
     7201 acattggtga gccttctgtc ctgaaccgtg caggtggtgc gccgacgctg gcggtaggta
     7261 tcgctcacgt gttccacaaa gtgctgccga tggctgacat gggcttctgg tatcacttcg
     7321 gtattctgtt cgaagccctg ttcatcctga ccgcgctgga tgcgggtacc cgttctggcc
     7381 gctttatgct gcaagacctg ctgggtaact tcatcccgtt cctgaaaaaa accgactctc
     7441 tggttgccgg tatcatcggt actgcgggct gtgtgggtct gtggggctac ctgctgtatc
     7501 agggcgtggt cgatccgctg ggcggcgtta agagcctgtg gccgctgttc ggtatctcca
     7561 accagatgct ggcagccgta gcgctggtac tgggcaccgt tgtgctgatt aagatgaagc
     7621 gcacccaata catctgggta actgttgttc cggctgtatg gctgcttatc tgcaccacct
     7681 gggcgctggg tctgaaactg ttcagcacca acccgcagat ggaaggcttc ttctacatgg
     7741 caagccagta caaagagaag attgctaacg gtactgacct gacggcgcag cagattgcca
     7801 acatgaacca catcgttgtg aacaactaca ccaacgcagg cctgagtatt ctgttcctga
     7861 ttgtggtgta cagcatcatc ttctacggtt tcaaaacctg gcttgcggtg cgtaacagcg
     7921 acaaacgtac tgacaaagaa acaccgtacg ttccaatccc ggaaggcggc gtgaagatct
     7981 cttcgcacca ctaaccgtgt ttagccccgc ttcggcgggg ctttgttctt atcagagtga
     8041 actatgtttg gtaacttagg acaggcaaaa aaatatctcg gccaggcggc gaagatgttg
     8101 attggtattc cagactacga caactacgtc gagcatatga agaccaacca tcccgacaag
     8161 ccgtacatga gctatgaaga attcttccgc gaacgccaga acgcgcgcta cggcggcgat
     8221 ggtaaaggcg gtatgcgctg ttgttaatgg agagagcatg aacccgattg cagttaccct
     8281 actcaccggt tttttaggcg cgggcaaaac taccctgctg cgccatattc ttaacgaaca
     8341 acacggctac aagattgccg tgattgaaaa cgaattcggc gaagtctccg ttgatgacca
     8401 actgattggc gaccgcgcca cgcagatcaa aacgctgacc aacggctgca tctgctgttc
     8461 gcgctccaac gagctggagg acgcactact cgacctgctg gataatctcg acaagggcaa
     8521 tattcagttc gatcgtctgg tgattgaatg caccggcatg gccgatcccg gcccgattat
     8581 tcagaccttt ttctcccatg agattttatg ccagctctac ctgctggacg gcgtgattgc
     8641 gctggtggat gcggtacatg ccgatgagca gatgaaccag ttcaccatcg cccagtccca
     8701 ggtgggctac gccgaccgta ttctgctgac taaaaccgac gtcgcaggcg aagcagagaa
     8761 actgcgtgaa cgcctggcgc gcatcaacgc ccgcgcaccg gtctacaccg tcacccacgg
     8821 cgacatcgac ctgggcctgc tgttcaacac caacggtttt atgctggaag agaacgtcgt
     8881 cagcaccaaa ccgcgtttcc actttatcgc ggataaacaa aacgatattt cgtcaattgt
     8941 ggtggaactg gattacccgg tagatatcag cgaagtttcc cgcgtgatgg aaaacctgct
     9001 gctggaatcg gcggataaac tgctgcgtta caaagggatg ctgtggattg acggcgaacc
     9061 taaccgcctg ctgttccagg gcgtccagcg cctctacagc gccgactggg acaggccgtg
     9121 gggcgatgaa aaaccgcata gcacaatggt gtttatcggg attcagttgc cggaggaaga
     9181 gattcgggcg gcgtttgcgg gattgaggaa gtaagttcgg gaaagatcaa ctttttgaga
     9241 gttccaccgc aacattatta cgagtggaac tctttagata tgtcagacca accaaagctt
     9301 cttcaattgc gttttcattt tttcatgtct cattttcagt tcagccaatc cccattctga
     9361 agaattacaa acatcttttg ttaaataaa
//
LOCUS       DAZXUK010000151         9374 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZXUK010000151 DAZXUK010000000
VERSION     DAZXUK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9374
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.151"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            211..1524
                     /gene="gltP"
                     /locus_tag="ACH3L8_002800"
     CDS             211..1524
                     /gene="gltP"
                     /locus_tag="ACH3L8_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFT4085842.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(1602..2291)
                     /gene="yjcO"
                     /locus_tag="ACH3L8_002801"
     CDS             complement(1602..2291)
                     /gene="yjcO"
                     /locus_tag="ACH3L8_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFT4085843.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(2385..4532)
                     /gene="fdhF"
                     /locus_tag="ACH3L8_002802"
                     /pseudo
     CDS             complement(2385..4532)
                     /gene="fdhF"
                     /locus_tag="ACH3L8_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(4730..6196)
                     /gene="mdtP"
                     /locus_tag="ACH3L8_002803"
     CDS             complement(4730..6196)
                     /gene="mdtP"
                     /locus_tag="ACH3L8_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFT4085844.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(6193..8235)
                     /gene="mdtO"
                     /locus_tag="ACH3L8_002804"
     CDS             complement(6193..8235)
                     /gene="mdtO"
                     /locus_tag="ACH3L8_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFT4085845.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            complement(8235..9266)
                     /gene="mdtN"
                     /locus_tag="ACH3L8_002805"
     CDS             complement(8235..9266)
                     /gene="mdtN"
                     /locus_tag="ACH3L8_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFT4085846.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(9285..>9374)
                     /locus_tag="ACH3L8_002806"
     CDS             complement(9285..>9374)
                     /locus_tag="ACH3L8_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085847.1"
                     /translation="RANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         2085 a         2513 c         2517 g         2259 t
ORIGIN      
        1 cattaatcct gccataaact cgcgttatta tgcattaatg cagcgaaaag ctctgttgtt
       61 aaagggttgc gcaacatacc gcgcaaatga tactgatcat aagcgttaaa aaaatctaca
      121 aacctacgca acacaattca tgccctggca gtatgtcacg ttctcgcgtt tctgaacggg
      181 gaacggcgct ccattgagga agttattctg atgaaaaata taaaattcag cctggcctgg
      241 cagattctgt ttgctatggt gctgggcatt ctcctgggaa gctacctgca ctaccatagc
      301 gacagccgcg actggctggt cgtcaatttg ctctctccgg cgggtgatat cttcatccat
      361 ctgatcaaaa tgattgttgt gccgattgtg atctccacgc tggtggtggg tatcgcgggt
      421 gttggtgatg ccaaacagct cgggcgtatt ggcgcgaaaa ccattattta cttcgaggtg
      481 atcaccaccg tcgccatcat tttggggatc actctggcga acgtcttcca gcccggtgcc
      541 ggggtggata tgtcgcagct ggcgaccgtc gatatctcga aatatcagag cactacggaa
      601 gcggtacaaa gcagttccca cggcattatg ggcacgattt tgtcgctggt gccgacgaac
      661 attgtggcgt cgatggcgaa aggcgaaatg ctgccgatca tctttttctc ggtgctgttt
      721 ggtctggggc tttcttccct gcccgcgacg catcgtgaac cgctggtgac cgtgttccgc
      781 tccatctctg aaaccatgtt taaagtgact cacatggtga tgcgttatgc accggtgggt
      841 gtgtttgcgc tgattgcggt gacggtggct aactttggtt tctcgtctct gtggccactg
      901 gcgaaactgg tgctgctggt gcatttcgcc attctgttct tcgcgctggt agtgctggga
      961 attgtggcac gtctgtgcgg gttaagcgtc tggatcctga ttcgtattct gaaagatgag
     1021 ctgattctgg cgtactccac tgccagctct gaaagcgtac tgccgcgaat tattgagaag
     1081 atggaagcct acggagcacc ggcgtcgatc accagcttcg tggtgccgac cggttactct
     1141 tttaaccttg atggttcgac gctgtatcaa agtattgccg ctattttcat cgctcagctg
     1201 tatggcattg acctgtccat ctggcaggaa atcattctgg tgctgacgct gatggtgacc
     1261 tcgaaaggga ttgctggcgt ggctggcgtg tcgtttgtgg tgttgctggc aacgctgggt
     1321 agcgtaggta ttccgctgga aggtctggcg tttattgctg gtgttgaccg tatcctcgac
     1381 atggcgcgta ctgcgctgaa cgtggtgggt aatgcgctgg cggtgctggt gattgccaag
     1441 tgggaacaca aatttgaccg caaaaaggcg ctggcttatg agcgtgaagt gctgggcaaa
     1501 tttgataaaa ctgcggatca ataattgaag attgccgggg atatcccccg gcaatgtgtg
     1561 gatgcctgat gcgacgcttg ccgcgtctta tcaggcctac cttaaccgtt ggttaatttt
     1621 tcaaactctt cacacccggt atcaaaccct tccatacagc tcaggttcaa ccagtgcagc
     1681 gccttttgtt tgttcttctc gataaaacct tcctcaccgt ttaagaacat cattcccgcc
     1741 cagtactcgg aataaccggt tcgggaaatt gcagagctgc gtttgaaata ccaggttgcc
     1801 ttgtcatcgt cggccttaat gcccacgccg ttggcgtaaa tcaaaccaag cagcatttgg
     1861 gcatcgacgg cggagtcatt ctccagatct tccgaggcgt tttctaacag cgaaatcgcc
     1921 tttggataat ccggtttacc cgcttgagta tttaccagaa tatgcgccag cgttacttca
     1981 ccttctttac tgcccgcttg cgccgctttc tctgcaagaa cttttgcttg tggatagtcc
     2041 agactgaccg gattggtgat tttgatctgc gccaacagcg cgcaggcatc agcatcaccg
     2101 ctgtcggcgg ctttctgtgc ccaatattcg gctttgctca aatcgccgga gctaaaccag
     2161 ctgtcggcaa gaaaatattg tgcacgtcgg tccccggcct ctgctgcctt taaatactgg
     2221 ctgccaggct cggagtcgtt cgcgtgggca aagaatgtca aaaacaacat caatgcgata
     2281 atttttttca ttttgataat acgattgggt agggagtaac cagtataaag agttcgaatg
     2341 gataaaaaaa cagcctccga aaggaggctg tagaaaggac tgtattacgc cagtgccgct
     2401 tcgcgcaggc gagttttcaa cttgttgtac tcgtcaatca cgtactgctc ggcggcgcgc
     2461 tgatcggaga tcggctcgac gcgcacggcg cagtatttgt attccggcgt tttcgtaatc
     2521 gggcttaagc tttcggtaac cagctcgtta caggcaccaa tccaccactg gtaggtcatg
     2581 taaatcgccc ctttgttcgg acgatcgctg acctgcgcac gggtgataat tttgccttta
     2641 cgcgagtgca ctcaaaccaa tgcctcatct tcaataccca gacgtttggc gtcttcggta
     2701 ttgatttgtg cgtagccagg ttcatcagcc agcgccgcca gtgccgcaca gttaccggtc
     2761 atcgaacggc aagagtagtg accaacttca cgcaccgttg acagtaccat cgggtactcg
     2821 tcggtgagtt tgtcgattgg cgctacccag tcgcaggtga agaactgcgc cagaccgttc
     2881 ggggtatcaa acttctcttt aaacagataa gaagtcccct gatcggcatc tgaagtatcg
     2941 cggcaaggcc actgaatgaa gcccagttcg cccattttct cgtaagtcgc accgtagaaa
     3001 tccgggcaca gatgacgcaa ctcatcccag atctcctggg tgttgttgta gtgcatcgga
     3061 taacccatac gggtggcgat ttcactgatg atttgccagt ccgttttcag atcccatttc
     3121 ggttcaactg ctttgaagaa acgctggaag ccacggtcag ccgcagtaaa cacgccttca
     3181 tgctcgcccc acgacgttga cggcaaaatg acatccgccg ccgacgcggt tttggttata
     3241 aagatgtcct gaacgatgac cagttccaga tcttcaaagg ctttacgtac tgccgacagc
     3301 tccgcatcag tttgcagcgg atcttcgccc ataatgtacg cggcacgcac ttcgccatgc
     3361 gctgcgcggt gcggcagctc gctgatgcga tagccggtat gagcaggcag gctttccacg
     3421 ccccaggctt tggcgaattt ttcgcggtta gccggatctt tcacgtactg gtatcccgga
     3481 tacgtatccg gcagcgcgcc catatcgcag gcaccctgca cgttgttctg accacgaacc
     3541 gggtttacac ccgcatgcgg cttaccgagg ttactggtca gcatcgcgag gctggtcaga
     3601 gaacgcacgg tttccacgcc ctggtagaac tgggttacac ccatgcccca caggatggcg
     3661 gcgcttttcg cctgggcata catccgtgcc gcctgacgaa tctcactggc gctgacaccg
     3721 gtgatatctt caaccgactc cggcgtgtag ccttcaacga ttttacgata ctcttcaaag
     3781 ccttctgtac gggaagcgac gaacgctttg tcgtacagat tttcttcaat aatgacatgg
     3841 cccatcgcat tcaacagcgc gatgttcgag ccgtttttca gcgcaatgtg catgtcagca
     3901 atgcgcgcgg tttcaatttt gcgcggatcg cagacgataa ttttcgcccc gttacgttta
     3961 gcgttaatta cgtgattcgc cacgattggg tgggaatccg ccgggttgta cccgaaaacg
     4021 aacactaaat cggtattatc aatttcgtta atagcattgc tcattgcgcc attaccgacc
     4081 gattggtgca gacctgcaac cgatgggccg tgtcagacac gagcgcagca gtcaacgtta
     4141 ttggtaccaa taacggcgcg cgcaaatttt tgcattacat agttggtttc gttacccgta
     4201 ccacgcgagg agccggtcgt ctggatggca tccggaccgt acttctcttt gatggcgctc
     4261 aggcgctcgg caacgtaatt cagtgcctca tctcaggaaa caggttcgag tttgccgcca
     4321 cgctggcgac ggatcatggg ggttttcagg cgcggggtca ggatctgggt atcgttaatg
     4381 aaatcccagc cataataacc cttcagacac agggtaccct ggttggtttt cccctgcgct
     4441 gcctccgccc ggacgatttt gccgttatcg acgaccaggt tgattttgca acctgatgcg
     4501 caataggggc aaaccgtgac gacttttttc atcggtctcg ctccagttaa tcaaatcacg
     4561 catacgcgct ctcgactaca gtatgcatct tttatgccac attttatttg gggtcattcc
     4621 ctgatattac gggcactatt tattcaaaac tctgacgaaa aacaggctgt cgtcagtttt
     4681 gacgtgacga aacgaaatac cgcgtgacag ccatcacgcg gcagacattt tattttttct
     4741 cgacgacggg agccgcctga tacccgccgc ccagcgattt catcaactga atgctttgga
     4801 tcacccggcg gctgtccagc atcagtaatg acatctcttc ggcaagtact ggcaaccggg
     4861 cttcagtggc ctgtaagcgg ctggttaagc cgcgctgata ggcggcctcg gcagcgcgct
     4921 gggtgaagcg cgttgcttcc acgtgttcag cctgcatttc tcgctcgtcg ttgagcgttt
     4981 gcagacgcgt gccgttgaca gcaacgtcac gcaccgcgtt cagtactgac tggttgtaac
     5041 gttcaatcat catgttgctg gcggcgcgcg tgccttcgag attggcattc aaccgtccac
     5101 cgtcaaacag cggcaatttc agacccggga tgaagttgaa ctggcgactg gtttttttga
     5161 ataaggtatc caggtggatg gcatcaaggc cgaaaaacgc tttgatatca aagctcggat
     5221 agaacaacgc ccgcgcggaa tccacctgat ctaatgacgc ctgaacatac cagcgcatgg
     5281 cttgcagatc cgggcgtctg gcgagcaact cataagagag tgtcgccgga atgccggtct
     5341 ggactcgtgg taatgccaca ggtttgatct ccggcatatc gctggctccc gcgccaatca
     5401 atgcgcgcag agattctctc gtttcggtga tttgcccttt gacggcagca atttgtttat
     5461 cgaccgccag aatctgtgcc cgcgcgccgt ggaaaggcac ttgtgcttcc agaccgtgcg
     5521 ccactttact ttggtgcgct ttcaccgcgt aatcaatcac atcgcgagtt tgttctaaca
     5581 gatcgagcat ctgatagctg gcctgcatac tgtaataaag ctgcgctacg cccgtggtca
     5641 gcgatagctc tactgctgcg gtttctgcca gcgcggcatt atgcgcgcca atggcggcgg
     5701 caaccgctga gcgatgcaca ccccacaaat caagatccag tccggcaaac aaacctactg
     5761 tggcttccgt atagtacggc ccgtccatcc ccagtgcggg cgcatccatc gcataagggc
     5821 ttaaaaagcc gttcgccgag acacgttggc ggttaagcat ccctaacgct gccacctgca
     5881 actgtgaacc ggcatctaac aaatcggcct gcgattgcgc tttttcttcc cgcagtttcg
     5941 cttcggcgag ggtgtgtgaa ccacttagcg tccgttggat cagggcatcc aactgcgggt
     6001 cattgagttg tttccaccac tgcgcctgcg gccagccgga gctggcaaga tgaatatcat
     6061 cggccagttt gatttgttcc ggtttgagct gttgatgagg cgcagaatcc ttacgtacca
     6121 gggcacagcc ggaaatcagc gtcgtgctgc cgagaatgct gcacaacagc agacgtgaaa
     6181 gttgacgatt gatcattgcg tggctccttg tgcctgttcc gtggcgggcg ttaatgccgg
     6241 ggctgtccag tctggcaagc gggcgaaaag ctggcagacg tgttgctcct gttttaataa
     6301 ggtgggcagg atggcctgcg aggtgggtgt ctcttcaagc gttatttgag gagaacggct
     6361 gagtgcggtt gccagaccgg cagcgtattt ctccagggcg tcggcaaact gacctgctct
     6421 gtccggctgt agtgcgttat ccagcgcctg cgccgaattc caggtggcat cccaggcgtg
     6481 aaggatatcg cggccctgac gaataaccgt ttgcgaacgt tcaatcagta aggcgcgttc
     6541 ttcgctgtcc agttgacgct ccagcgccac gcgttggcac atctcttcac aggcattaaa
     6601 cgccgcatgc agaccgatac gaatttgcag ataagtgcgc agagccgtga cttcctgctg
     6661 gcgtgggatc cgcattactt tacttaacat acccagcgcg ccagccagtt tttgcggcag
     6721 cgtgcgcgct tcactttcag gccagacaaa ggtgtaaatc atcgcggaca ccacggtacc
     6781 aatgatgata cccagggcgc gatcgcgaat ttccaccagg tcgtacactg ggccaaaaac
     6841 gttttcgagc gtggcgagcg cgaaggtgac caccatctgt gtgccgatat aagaagagcg
     6901 ttcagagctg gtggcaatcc atgcgcccaa caggaaaatc ggtgccagca caaacagcaa
     6961 ttcgacaata ttgtccagcc agggcatgac cagtagcgtg aataacagcg ccagaatcgc
     7021 gccgcaaaag gccccgccaa aacgcagcac catcttctgg tacgacgaac cgacatttgg
     7081 attagcgacg atcacgcagg tcagcataca ggtgtgaatg ccttcccagt ccacgccgct
     7141 gtaaaaggtg taacagatca aacaggcgag caatgttttt accgcgtagc gtatatagtc
     7201 tggattggta aaagcatcgg cgaccattga tggcggtttg gcggcgggcg ttggcggcgt
     7261 attcgggttc atctggccca gttgtaacaa cgtctggcag atattctcca ggttacattc
     7321 ccgtgccgcc accgcttcac tttcactgag ccgccagtcg ctttgccagc actgaccttc
     7381 agcaacggca tgctgcagct tgttgatttc tgaggctaat ttttgccgaa attcaataat
     7441 tgcctgagaa tcagcaaaag aggtgggatc gtagcgattc agcgtcgagt aaatgtaggt
     7501 taccgttgcc acgcagcttt gccaccatgc gctttgagtt cgccagttgg catcgtccgc
     7561 gagacaaaag acattcagtt tttgcagcgc cagcgcctct ctttcaatcc gcgtttcggg
     7621 tagcggtgcg aggctgtccg tcaggtggct aatggcatca tcaagccgat cattaagcgc
     7681 ttgatgcatt tgcgtaatgg cacgattggg aaaccacagc acgccgatta acgtcatcag
     7741 caaggttgga tagaggccaa caacgataca ccacagcgtt aagcgcacga ctacttccgg
     7801 atagtcgagc atggcgggga aggtttgccc gtaaatggcg acagcgaaaa agaccagtcc
     7861 cagccgatgg gtgcgcatca aaaacatgca gcccatcagg atcggcccgg cgatgatcaa
     7921 tcggatcaac ggttcgccgt atgaccattt gtagatcaaa aacaggctgc cgatctccag
     7981 caccgtggca accacaaaca agatcgccac aaatttggtg taaaacgcgt tcgactgaat
     8041 accgtaaaac agcactgcca gcgataacgc cacaaaaggg atctcaaaag tcatcgagat
     8101 cagaatcacc agcaggcagc ctacccagag ttgcacgatc tgcggcacgc ggccgggtcg
     8161 ccgctcgctt aactcttcat gaaagaacgc cagcagcctg accaccggta atggcaggga
     8221 gttgagcgcg ctcattattg cggctcaaga ttagcgactg ccgaagcgcc gatgcggaac
     8281 atttccgggt caggtttatc gaccatgatt ttgaccggaa aacgctgggc aacgcggacc
     8341 cagttaatag aacgagacac ttttggcagg ccgcccagca ccaggccgcc gtcatccggt
     8401 agcacgccgt agccaatcga atccacttta ccctcgaagg ttttgccgct gtcactcatc
     8461 aggcgaatcg ttgcgggtgt acctgagcga atatttttca gatcggtttc gcggaagttg
     8521 gcgatcacgt accagtgacg agtgtcgatg agggtaaaaa tagggcgcat ggcagaagca
     8581 aattgcccga cggaggtttt gagggaaatg atccggccat caaatggcgc gcgaacggtc
     8641 gtcatttcca gatgtagttt ggtcagggca atatccgctt cgaccgccgc acgttgcgcc
     8701 accagcgcat cgacgccgct gacggcgctg gcggctgact gcgcctgtaa caatacggca
     8761 ttaagatccg cttctgcggc gcgctgcgcc gttcttgcac ggtcaacgtc ttctgctgag
     8821 acaaaaccct ctttcagtaa tggctcggtg cggcgtaatg tatctgtggc ctgtttcgcg
     8881 gcggcacggg ctttttctac cgtggcatta accgagtcgg caccaaactg ttgggcgtca
     8941 acgctacgct gggtgagcat aatttgctta tccagcgccg cgagggaggc ttcggctttc
     9001 gccaggttgg cttcgtacgg gcgcgggtcg atgcggaaca gcaaatcgcc ctgtttcacc
     9061 gcctggttgt cggtgaccgc cagttctaca atgcgaccgc tgacttccgg caccacatca
     9121 atggtatctg ctgacgcgta agcgtcatta gttgatggcg cactgtctac gcgccagata
     9181 acgaaaacaa gggcaaccag cgccaacgca accactaaca gagcagggaa tttactgcga
     9241 ggtgcttttt tcggcgtact ttccatgagc gcgtctcaaa tagattagaa aaatgccagc
     9301 cagaacagca tggcgtagag agcaaaaagg gcggtataaa taattccgac aaatgccaga
     9361 ttgatatttg cccg
//
LOCUS       DAZXUK010000152         9347 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZXUK010000152 DAZXUK010000000
VERSION     DAZXUK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9347
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.152"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="ACH3L8_002807"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="ACH3L8_002807"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFT4085848.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="ACH3L8_002808"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="ACH3L8_002808"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFT4085849.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="ACH3L8_002809"
     CDS             3115..3399
                     /locus_tag="ACH3L8_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFT4085850.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACH3L8_002810"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACH3L8_002810"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFT4085851.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACH3L8_002811"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACH3L8_002811"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFT4085852.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACH3L8_002812"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACH3L8_002812"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFT4085853.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="ACH3L8_002813"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="ACH3L8_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFT4085854.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2226 a         2471 c         2381 g         2269 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgaccccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtcta
//
LOCUS       DAZXUK010000153         9326 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZXUK010000153 DAZXUK010000000
VERSION     DAZXUK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9326
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.153"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="ACH3L8_002814"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="ACH3L8_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFT4085855.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="ACH3L8_002815"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="ACH3L8_002815"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFT4085856.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="ACH3L8_002816"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="ACH3L8_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4043)
                     /gene="rhaR"
                     /locus_tag="ACH3L8_002817"
     CDS             complement(3195..4043)
                     /gene="rhaR"
                     /locus_tag="ACH3L8_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFT4085857.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="ACH3L8_002818"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="ACH3L8_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFT4085858.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="ACH3L8_002819"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="ACH3L8_002819"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFT4085859.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7925
                     /gene="rhaA"
                     /locus_tag="ACH3L8_002820"
                     /pseudo
     CDS             6707..7925
                     /gene="rhaA"
                     /locus_tag="ACH3L8_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
     gene            8230..8991
                     /gene="rhaD"
                     /locus_tag="ACH3L8_002821"
     CDS             8230..8991
                     /gene="rhaD"
                     /locus_tag="ACH3L8_002821"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFT4085860.1"
                     /translation="MKGWDERNGGNLTLRLDDADIALYHDNFHPQPRYIPLSQPMPLL
                     ANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTNEAVPTSELPAHFL
                     SHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVG
                     ILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDEAFGLIDTAEKSAQVLV
                     KVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            9001..9315
                     /gene="rhaM"
                     /locus_tag="ACH3L8_002822"
     CDS             9001..9315
                     /gene="rhaM"
                     /locus_tag="ACH3L8_002822"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFT4085861.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2255 a         2451 c         2449 g         2171 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataggccca ttgtttttat tttggaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgc gttgctggaa cctaccgctg
     7741 agctgcgcaa gctggaagcg gcgggcgatt acactgcgcg tctggcactg ctggaagagc
     7801 agaaatcgtt gccgcggcag gcggtctggg aaatgtattg ccaacatcac gatacgccag
     7861 caggtagcga atggctggag agcgtgcggg cttatgagaa agcgattttg agccagcgtg
     7921 ggtaaacact gccggatgcg gcacgagcgc cttatccggc ctacgggtcg aatgtccatg
     7981 cgggctgtca ccctcacccc ggccctctcc ctgaaagggc gagggagaaa aacatagcct
     8041 cgtgcggcaa cggagctgaa caccgctccg gacagtcccc tcgcccctac ggggagaggg
     8101 ttagggtgag gggaacaagc cgcaccatgt ctaaacaagg caacctaatt tacaggaaca
     8161 cagaacatgc aaaacattac ccagtcctgg tttgtccagg gaatgatcaa agccaccacc
     8221 gatgcctagc tgaaaggctg ggacgagcgc aacggcggca acctgacgct gcgcctggat
     8281 gacgccgata tcgcgcttta tcacgacaac ttccacccac aaccgcgcta tattccgctc
     8341 agccaaccca tgcctttact ggcaaatacg ccgtttattg tcaccggctc cggcaaattc
     8401 ttccgtaacg tccagctcga tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat
     8461 ggcgcgggct accatattct ctgggggtta accaacgaag ccgttccaac ttctgaactt
     8521 ccggctcact ttctctccca ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg
     8581 atcatgcact gccacgccac caacctgatc gccctcacct atgtactgga aaacgacacc
     8641 gcggtcttta ctcgccaact gtgggaaggc agcaccgaat gtctggtagt attcccggat
     8701 ggcgttggca ttctgccgtg gatggtgccc ggcacggacg aaatcggcca ggcgaccgca
     8761 caagagatgc aaaaacattc gctggtgttg tggcccttcc acggtgtgtt cggttccgga
     8821 ccgacgctgg atgaagcctt tggtttaatc gacaccgcag aaaaatcagc acaagtatta
     8881 gtgaaggttt attcgatggg cggcatgaaa cagaccatca gccgtgaaga gttgatagcg
     8941 ctcggcaagc gcttcggcgt tacaccacta gccagtgcgc tggcgctgta aggagcaaac
     9001 atgatccgca aagcctttgt catgcaggta aaccccgacg cccacgaaga gtatcagcgt
     9061 cggcataatc ccatctggcc ggaactggaa gcagtgttga aatctcacgg tgtgcataac
     9121 tacgccattt acctcgacaa agcgcgtaat ctgctgtttg ccatggtaga gattgaatct
     9181 gaagaacggt ggaatgcggt agccagtact gatgtttgcc aacgttggtg gaaatatatg
     9241 accgatgtta tgcccgctaa cccagataac agcccggtga gtagcgagct gcaagaagtg
     9301 ttttacctgc cgtaattgcc caatgt
//
LOCUS       DAZXUK010000154         9317 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZXUK010000154 DAZXUK010000000
VERSION     DAZXUK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.154"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..290
                     /locus_tag="ACH3L8_002823"
     CDS             <1..290
                     /locus_tag="ACH3L8_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFT4085862.1"
                     /translation="QQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGI
                     SESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
     gene            290..5560
                     /gene="traI"
                     /locus_tag="ACH3L8_002824"
     CDS             290..5560
                     /gene="traI"
                     /locus_tag="ACH3L8_002824"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFT4085863.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRTATSEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5580..6326
                     /gene="traX"
                     /locus_tag="ACH3L8_002825"
     CDS             5580..6326
                     /gene="traX"
                     /locus_tag="ACH3L8_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFT4085864.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            6381..6941
                     /locus_tag="ACH3L8_002826"
     CDS             6381..6941
                     /locus_tag="ACH3L8_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFT4085865.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGETPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            7073..7285
                     /locus_tag="ACH3L8_002827"
     CDS             7073..7285
                     /locus_tag="ACH3L8_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFT4085866.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
     gene            7531..7992
                     /locus_tag="ACH3L8_002828"
     CDS             7531..7992
                     /locus_tag="ACH3L8_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFT4085867.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            8038..8247
                     /gene="hha"
                     /locus_tag="ACH3L8_002829"
     CDS             8038..8247
                     /gene="hha"
                     /locus_tag="ACH3L8_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFT4085868.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            8285..8875
                     /locus_tag="ACH3L8_002830"
     CDS             8285..8875
                     /locus_tag="ACH3L8_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFT4085869.1"
                     /translation="MKLIIFILIVLIIAALLIRIILKSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            9030..>9317
                     /locus_tag="ACH3L8_002831"
     CDS             9030..>9317
                     /locus_tag="ACH3L8_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085870.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNEDAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMM"
BASE COUNT         2236 a         2221 c         2968 g         1892 t
ORIGIN      
        1 cacaacagcc agtgtcttct gtcatcaatg ataagaagtc agatgcaggt gtgagtgttc
       61 cggcaggggg gatcgagcag gagctgaaaa tgaaaccgga agaagagatg gaacagcaac
      121 tgccacccgg gatcagtgaa tccggtgaag tggtggatat ggccgcttat gaggcatggc
      181 aacaggaaaa tcatccggac atccagcagc atatgcagcg tcgtgaagag gtgaacatta
      241 atgtgcaccg ggagcgcggg gaggatgttg agccgggaga tgatttctga tgttgagttt
      301 cagcgttgtt aaatcggcag gcagtgccgg aaactattac accgacaagg ataattacta
      361 tgtgctgggc agcatgggag aacgctgggc cggtcagggg gctgaacagc ttgggctgca
      421 gggcagcgtc gataaggatg tttttacccg tcttctggag ggcaggctgc cggacggggc
      481 ggatttaagc cgcatgcagg atggcagtaa taagcatcgt cccggctacg acctgacctt
      541 ctccgccccc aaaagtgtct ccatgatggc catgctcggg ggggataaac gtcttattga
      601 tgcacataac caggccgtgg attttgctgt ccgtcaggtg gaggcgctgg cttccacacg
      661 ggtgatgacg gacggacagt cagaaacggt gctgaccggt aatctggtga tggcactgtt
      721 taaccatgac accagtcgcg atcaggaccc acagttacac acgcatgtgg tggtggctaa
      781 tgtcacgcag cataacggcg agtggaagac actgagcagt gacaaagtgg ggaaaaccgg
      841 attttccgag aacgtgctcg cgaaccggat tgcgttcggg aaaatatatc agagtgagtt
      901 acgtcagcgc gttgaggcgc tgggttatga aaccgaagtg gtcggtaagc acggtatgtg
      961 ggagatgccg ggcgtaccgg tggaggcctt ttccagtcgt tcacaggcta tcagggaggc
     1021 cgtcggtgaa gacgcctcgc tgaaatcccg ggatgtggcg gcactggata cgcgaaaatc
     1081 caaacagcac gtcgatccgg aaatcagaat ggctgaatgg atgcagacgc tgaaggaaac
     1141 cgggttcgac atccgggcat atcgtgacgc ggcggatcag cgtgcggaga tccgcacgca
     1201 ggcgcccggg cctgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggcgat
     1261 tgccggatta agtgaacgaa aagtgcagtt cacgtatacg gacgtgctgg ccaggacggt
     1321 cggcatactg ccgccggaaa atggtgtgat tgaacgggca cgcgccggta tcgatgaggc
     1381 catcagccgt gagcagctta tccccctcga ccgtgagaag gggctgttca catcaggaat
     1441 tcatgtgctc gatgagctgt ccgtccgggc actcagtcgt gacatcatga aacagaaccg
     1501 ggtgaccgta catccggaga aaagtgtccc ccggacggct ggttacagcg atgcggtcag
     1561 tgttctggca caggaccgtc cgtcgctggc cattgtgtcc gggcagggcg gtgcagccgg
     1621 gcagcgtgag cgggtggctg aactggtcat gatggcccgg gaacaggggc gggaggtgca
     1681 gattatcgct gctgaccgtc gctcgcagat gaacctgaag caggatgaac ggctgtccgg
     1741 tgaactgata accggacgtc gtcagctgct ggaaggcatg gccttcacgc cgggcagtac
     1801 cgttatcgtc gaccagggtg aaaaactctc cctgaaagag acgttaaccc tgctggatgg
     1861 tgccgcacgt cataacgtac aggtcctgat aaccgacagc gggcagcgaa ccggtacagg
     1921 cagtgcgctg atggccatga aggatgccgg ggtgaacaca tatcgctggc agggcggaga
     1981 acagcgaccg gccaccatca tcagtgaacc ggaccgtaat gtccgctatg cccggctggc
     2041 cggagatttt gcggccagcg tgaaagccgg agaagagagc gtggcacagg tcagcggggt
     2101 acgggaacag gccatactga cacaggccat tcgcagtgag ctgaaaacgc agggcgtgct
     2161 cggacaccct gaggtgacca tgactgcact gtcaccggtc tggctggaca gccggagccg
     2221 ttatctgcgg gatatgtacc gaccggggat ggtgatggag cagtggaacc cggagacacg
     2281 cagtcatgac cgctatgtta tcgaccgggt gacggcgcag agtcacagcc tgaccctgcg
     2341 ggatgcgcag ggcgaaacgc aggtggtgcg tatttcctcc ctggacagca gctggtcgct
     2401 gttccggccg gaaaaaatgc cggtggcaga cggcgagcga ctgagggtga cagggaaaat
     2461 tcccggactc cgcgtctccg gcggtgaccg cctgcaggtg gcatccgtca gtgaagatgc
     2521 gatgacggtt gttgtgccgg ggcgggctga accggccacc ctgcctgtgg ctgattcacc
     2581 gttcacggca ctgaagctgg agaacggctg ggtggaaacg cccgggcatt ccgtcagtga
     2641 cagtgcgacg gtgtttgcct ccgtcacaca gatggcaatg gacaacgcca ccctgaacgg
     2701 tctggcccgc agcggtcgtg atgtccggct gtattcctca ctggatgaaa cccgtactgc
     2761 ggaaaaactc gcccgccatc cgtcctttac ggtggtttct gagcagataa aggcgcgtgc
     2821 cggtgagaca ttgctggaaa ccgctatcag tctgcagaaa gccgggcttc acacgccggc
     2881 acagcaggcc attcatctgg cccttcctgt gctggaaagt aaaaacttgg ccttcagcat
     2941 ggtggacctg ctgacagagg cgaagtcgtt tgctgcagaa ggaaccggtt ttactgaact
     3001 gggaggggaa atcaatgcgc agataaaacg cggtgactta ctgtatgtgg atgtggcaaa
     3061 aggctatggc acaggcctgc tggtttcccg tgcgtcgtat gaggcagaaa agagcattct
     3121 tcgccatatt ctcgaaggca aggaggcggt cacgccgctg atggagagag tacccggcga
     3181 actcatggag acgttaacgt cgggacagcg tgccgccacc cgtatgatac tggaaacgtc
     3241 cgaccgtttc acggtggtac aggggtatgc cggtgtgggt aagaccactc agttccgggc
     3301 ggtgatgtca gccgtgaaca tgctgccgga gagtgagcgt ccccgggttg tggggctggg
     3361 gcccacgcac cgtgcggtcg gtgagatgcg cagcgccggc gtggatgcac agacactggc
     3421 gtcctttctg catgacacgc agctgcagca gcgcagcgga gaaacgccgg atttcagcaa
     3481 cacgctgttt ctgctcgatg agagctcaat ggtgggcaat accgacatgg cacgggcata
     3541 cgccctgatt gcggccggtg gcggtcgtgc tgtggccagc ggtgacacgg accagctgca
     3601 ggccatcgcg cccggtcagc ctttccgtct ccagcagacg cgcagtgctg ccgatgtggc
     3661 catcatgaag gagattgtgc gtcagacgcc ggaactgcgg gaggcggtat acagcctgat
     3721 taaccgggat gtggaaaggg cactgtccgg gcttgagagt gtgaaaccgt ctcaggtgcc
     3781 acgtctggag ggcgcatggg caccggagca ctccgtgacg gagttcagtc acagccagga
     3841 agcgaaactg gcagaagcgc agcagaaggc gatgctgaaa ggcgaggctt tcccggatat
     3901 ccccatgaca ctgtatgagg ccattgtccg cgactatacc ggcaggacac cggaagcacg
     3961 ggagcagacg ctgattgtca cgcacctgaa tgaggaccgg cgcgtactga acagcatgat
     4021 tcatgatgca cgggaaaagg ccggtgagct gggcaaagag caggtcatgg tgcctgtcct
     4081 gaacacagcg aatatacgtg acggagaact gcgtcgtctc tccacctggg agaaaaaccc
     4141 ggatgccctt gccctggtgg atagtgtgta tcaccggatt gccggtatca gcaaggatga
     4201 cgggctgata accctgcagg atgcggaagg taacacgcgg ctgatttcgc cccgggaggc
     4261 ggtggctgaa ggtgtcacac tgtacacccc ggacaaaatc cgggtgggga ccggcgaccg
     4321 gatgcgcttc acgaagagtg accgggagcg cggttatgtg gccaacagcg tctggacggt
     4381 gacagcggtt tccggtgaca gtgtcacgct gtcggacgga cagcagaccc gggtgattcg
     4441 ccccggtcag gagcgggcag agcaacatat tgacctggcc tatgccatca ctgcccacgg
     4501 tgcgcagggg gcgagtgaaa cctttgccat cgcgcttgaa ggcacggaag gtaaccggaa
     4561 acagatggcc ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca
     4621 ggtgtacacc gataaccgtc agggctggac ggatgccatt aacaatgccg tacagaaagg
     4681 aactgcccac gatgtgctgg agccgaaacc ggaccgggag gtcatgaatg cgcagcggct
     4741 gttcagtacg gcacgggaac tgcgggacgt ggcggcaggc cgtgccgttc tccgtcaggc
     4801 ggggctggcc gggggagaca gtccggcacg gtttattgca ccggggcgca aatatccgca
     4861 gccgtatgtg gcactgcctg cttttgaccg taacggcaag tccgccggta tctggctgaa
     4921 cccgctgacc acggatgacg gaaacgggct gcggggattc agcggtgaag gacgggtgaa
     4981 aggcagcggg gatgcgcagt ttgtggccct gcagggcagc cgtaacggcg agagcctgct
     5041 ggctgataat atgcaggatg gtgtccggat tgcccgtgat aaccccgaca gcggtgtggt
     5101 ggtgagaatc gccggtgaag gtcgtccgtg gaatcccggt gccatcaccg gtggtcgcgt
     5161 gtggggggat atcccggaca acagcgttca gccgggagcc ggaaatggcg aaccggtcac
     5221 ggcagaggta ctggcacagc ggcaggctga agaggccatc cgccgtgaaa cggaacgtcg
     5281 cgcagatgaa attgtccgta aaatggcaga gaacaaacct gacctgccgg acggcaaaac
     5341 agagcaggct gtcagggaga ttgccggaca ggagcgtgac cggactgcca cttctgaacg
     5401 ggaagccgcg ctgccggaga gtgtgctgcg tgaatcacaa cgggagcggg aagcggtccg
     5461 ggaggttgcc cgggaaaatc tgctgcagga gcgtctgcag cagatggagc gggatatggt
     5521 tcgtgacctg cagaaagaga aaaccctggg cggagactga tacaggaaga taaacgctga
     5581 tgacaacgga taacacgaac acgacacgta acgattcact ggctgcacgg accgatacct
     5641 ggttgcagtc atttctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac
     5701 tggtgctgat ggtactggac cacatcaatc tgatattcca gctgaagcag gaatggatgt
     5761 ttctggccgg gcgaggagcc tttccgctgt ttgccctggt gtgggggctg aatctgtccc
     5821 gtcatgcgca tatccggcaa ccggccatta accggctgtg gggatggggg attattgccc
     5881 agttcgcata ttatctggca ggctttccct ggtatgaggg gaatatcctg tttgcctttg
     5941 cagtggcggc acaggtgctg acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg
     6001 ccattcttct gatggccctg tgggggcctt tgtccggcac cagttacggc attgccgggc
     6061 tgctgatgct ggcagtcagc caccggctgt accgggcgga agacagagcg gaacgtctgg
     6121 cgctggtcgc ctgtctgctg gctgttattc cggcgcttaa ccttgccacc agtgatgcag
     6181 cggcggtggc tggtcttgtg atgacggtgc tgaccgttgg tctggtgtcg tgtgcaggaa
     6241 aatcattacc ccgtttctgg cccggggatt ttttcccggt attctacgcc tgtcatctgg
     6301 ctgtgctggg cgttctggcg ctgtgacggg tgtggtatct ttgaccgtaa caggatgatt
     6361 cgtcagaggc agaacacagc atgacagagc agaagcgacc ggtactgaca ctgaagcgaa
     6421 aaacggaagg ggagacgcct gtccgcaggc ggaaaaccat catcaatgtc accacgccac
     6481 caaagtggaa ggtgaaaaag cagaagctgg ccgagaaagc agcccgggaa gcggagctgg
     6541 cggcaaaaaa agcgcaggcc agacaggcgc tgtccattta tctgaacctg ccctcgctgg
     6601 atgaggccgt gaacaccctg aaaccctggt ggccgggatt atttgacggt gaaacgcccc
     6661 ggcttctggc ctgcggtatc cgggacgtgt tactggaaga cgtggcgcag cggaatatcc
     6721 cgctctcgca taaaaaactg cgcagggcgc tgaaggccat cacccgttca gaaagctatc
     6781 tgtgtgccat gaaagccggt gcctgccggt atgatacgga agggtatgtg acggagcata
     6841 tttctcagga ggaggaagcg tatgcggcag cgcgtctgga taaaatccgc cgccagaaca
     6901 ggataaaggc agagcttcag gccgtgcttg atgagaaata aaaaaaagcc tccatcagag
     6961 aggaggcagg gaaataaggc ttaaaaggaa ataacttccg tacccgaaag gaagccaatt
     7021 cagtagatta tttaacacca taatactgac gggttaaaga ggagtgataa agatgaatgg
     7081 attcagaaac agttcacgga acggtcaggt ctggcgctgg cagcgtgccg gaagccgggc
     7141 cgttattctg gaggtcagtg gacgctggat ggaagcggca gaagcatggc gacgggctgc
     7201 cggtgttgcg ccccggacag actggcagca gtttgcccga aaaagggctg aacactgcca
     7261 ccggcgcagc cggggaaggg tgtaacgaaa aaagcagcct tcattactcc agggattcag
     7321 aaggctgaat aatgcatgag atttgctttt tttaatatcc ggtcgggcag tatgaccgga
     7381 cagttctgag tagcacagag ttttttccgt attaagtcgt gattttatat tttgtgatta
     7441 atttcacaaa ataaggtgtt attcagtgtg tgctgcgata ttcaggatta cctgaaatac
     7501 aggtgccatt tatctgatat ggagaataac atgaggaaat atattccact ggtattattt
     7561 atcttttcat ggccggtatt gtgtgcagat attcatggac gggttgttcg tgtactggac
     7621 ggtgatacca tcgaggtcat ggactcacgg aaggcagtgc ggatcaggct ggtaaatatt
     7681 gatgcaccgg aaaagaaaca ggattatggc cggtggtcaa cggacatgat gaaatcactg
     7741 gttgcaggaa aaacggttac cgtcacatat tttcagcgag atcgttatgg tcgtattctc
     7801 gggcaggttt atgcaccaga cggaatgaat gttaatcagt ttatggtccg ggccggggct
     7861 gcctgggtat atgagcagta caacacagat cccgtgctgc ctgtccttca gaacgaagcc
     7921 agacaacaaa aacgtgggct ctggtcagat gctgatccgg ttcccccctg gatatggaga
     7981 caccgtaaat aaagcgatgg caatcttatc attcatctgt tctcagcggg aataaaaatg
     8041 gcgaaaacaa aacaggaatg gctgtatcag ttacggcgat gttcatcagt caatacgctg
     8101 gaaaaaatta ttcataaaaa cagagacagt ttattaaaca gtgaacgtga atcatttaat
     8161 tcagcagcag accaccggtt agcggaatta atcaccggaa agttatatga caggatacca
     8221 aaagagatat ggaaatatgt aaggtaataa ttcagaataa ttaattacca ggagcagata
     8281 aacaatgaag ctgattattt ttattctcat tgttctgatt attgcagcgt tacttatccg
     8341 gattatcctc aaaagtgtaa atcagcattc tcctttgctg atgcagttgc atgcggcagg
     8401 tatacggacc ggtgatgcag agcgaatact gtccggcggt gaatactggc aacgtcagaa
     8461 gacgctgctg acagaaaggg aagtcagttt tatgaaagga ctgttcagaa ttgtggatat
     8521 gaagcggtgg tatctgtgtc cgcaggtacg ggtcgcggat atcgtccagc tgaacgggaa
     8581 tatccggcca cgatcacgcc agtggtggca gttattcagg atggtgtctc agtggcatgt
     8641 tgatgtggtt atcgttgaac ggcgttcgtt cagtattgtt gcagcagtag agctggatga
     8701 tgccagccat ttacgaccgg aacgccggcg ccgggatatt cttctggaag aggttctgag
     8761 gcaggctggt attccgttgc tcagaagcca cgatgccaga aaactgctgc agatgaccgg
     8821 agaatggctg aatacaacag gggctgatca gcagtccccg gaacatcgta gctgacgcct
     8881 tcgcgttgct cagttgtcca accccggaaa cgggaaaaag caagttttcc ccgctcccgg
     8941 cgtttctgta ataaaaagcg ggaaatggtg gtcacagagt aaattccgga accacctgta
     9001 cccaaacttt atataataat ggtgaatata tgaaatatga tgatgtatca gaattgatga
     9061 ttaagcgtga acacaatatt cttattgttg ggaactctgc ctcccacatt gataagtttt
     9121 ttaaggggta ttctatacca ggttctaaat attatgattt cactcagatt aacagtgatt
     9181 cagatgtaga gagaaatgag gatgctgtct cctttatcag ggatgcaatg aatagtgaag
     9241 agcagacaat catttttaat tgtgctgggt ggcctgattt aggtggtgga tcagcagtat
     9301 cacagtttgc aatgatg
//
LOCUS       DAZXUK010000155         9215 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZXUK010000155 DAZXUK010000000
VERSION     DAZXUK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9215
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.155"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACH3L8_002832"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACH3L8_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFT4085871.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACH3L8_002833"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACH3L8_002833"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFT4085872.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACH3L8_002834"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACH3L8_002834"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFT4085873.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACH3L8_002835"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACH3L8_002835"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFT4085874.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="ACH3L8_002836"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="ACH3L8_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFT4085875.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /gene="smg"
                     /locus_tag="ACH3L8_002837"
     CDS             5562..6035
                     /gene="smg"
                     /locus_tag="ACH3L8_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFT4085876.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="ACH3L8_002838"
     CDS             6064..6606
                     /locus_tag="ACH3L8_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFT4085877.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="ACH3L8_002839"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="ACH3L8_002839"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFT4085878.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="ACH3L8_002840"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="ACH3L8_002840"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFT4085879.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="ACH3L8_002841"
     CDS             8003..8260
                     /locus_tag="ACH3L8_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFT4085880.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="ACH3L8_002842"
     CDS             complement(8236..8790)
                     /locus_tag="ACH3L8_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFT4085881.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2323 a         2232 c         2294 g         2366 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagaga aaaaatcctg aaattcaggg ttgactctga
     9061 aagaggaaag cgtaatatac gccacctcgc gacagtgcgc taaagcgcgt cgcaactgct
     9121 ctttaacaat ttatcagaca atctgtgtgg gcactcgaag atacggattc ttaacgtcgc
     9181 aagacgaaaa atgaatacca agtctcaaga gtgaa
//
LOCUS       DAZXUK010000156         9167 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZXUK010000156 DAZXUK010000000
VERSION     DAZXUK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9167
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.156"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..923
                     /locus_tag="ACH3L8_002843"
     CDS             <1..923
                     /locus_tag="ACH3L8_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4085882.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="ACH3L8_002844"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="ACH3L8_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFT4085883.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACH3L8_002845"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACH3L8_002845"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFT4085884.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="ACH3L8_002846"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="ACH3L8_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFT4085885.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="ACH3L8_002847"
     CDS             4446..4805
                     /locus_tag="ACH3L8_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFT4085886.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACH3L8_002848"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACH3L8_002848"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFT4085887.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="ACH3L8_002849"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="ACH3L8_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFT4085888.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="ACH3L8_002850"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="ACH3L8_002850"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFT4085889.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2094 a         2219 c         2476 g         2378 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg acgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa
     9121 atgcttgact ctgtagcggg aaagcgtatt atgcacaccc cgcgcag
//
LOCUS       DAZXUK010000157         9162 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZXUK010000157 DAZXUK010000000
VERSION     DAZXUK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9162
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.157"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            51..1142
                     /gene="rutA"
                     /locus_tag="ACH3L8_002851"
     CDS             51..1142
                     /gene="rutA"
                     /locus_tag="ACH3L8_002851"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFT4085890.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            1142..1834
                     /gene="rutB"
                     /locus_tag="ACH3L8_002852"
     CDS             1142..1834
                     /gene="rutB"
                     /locus_tag="ACH3L8_002852"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFT4085891.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            1846..2232
                     /gene="rutC"
                     /locus_tag="ACH3L8_002853"
     CDS             1846..2232
                     /gene="rutC"
                     /locus_tag="ACH3L8_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFT4085892.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            2240..3040
                     /gene="rutD"
                     /locus_tag="ACH3L8_002854"
     CDS             2240..3040
                     /gene="rutD"
                     /locus_tag="ACH3L8_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFT4085893.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            3050..3640
                     /locus_tag="ACH3L8_002855"
     CDS             3050..3640
                     /locus_tag="ACH3L8_002855"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFT4085894.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            3651..4145
                     /gene="rutF"
                     /locus_tag="ACH3L8_002856"
     CDS             3651..4145
                     /gene="rutF"
                     /locus_tag="ACH3L8_002856"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFT4085895.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            4166..5494
                     /gene="rutG"
                     /locus_tag="ACH3L8_002857"
     CDS             4166..5494
                     /gene="rutG"
                     /locus_tag="ACH3L8_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFT4085896.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(5576..5683)
                     /locus_tag="ACH3L8_002858"
     CDS             complement(5576..5683)
                     /locus_tag="ACH3L8_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFT4085897.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            6056..6652
                     /gene="wrbA"
                     /locus_tag="ACH3L8_002859"
     CDS             6056..6652
                     /gene="wrbA"
                     /locus_tag="ACH3L8_002859"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFT4085898.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            6673..6900
                     /locus_tag="ACH3L8_002860"
     CDS             6673..6900
                     /locus_tag="ACH3L8_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFT4085899.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(6938..8179)
                     /gene="agp"
                     /locus_tag="ACH3L8_002861"
     CDS             complement(6938..8179)
                     /gene="agp"
                     /locus_tag="ACH3L8_002861"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFT4085900.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(8472..>9162)
                     /locus_tag="ACH3L8_002862"
     CDS             complement(8472..>9162)
                     /locus_tag="ACH3L8_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085901.1"
                     /translation="FIQSEINKTYGLKYAPVDGDGYCLLRAILVLKEHEYSWALGSHK
                     TQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQSDNKIQGVGSFMSWSF
                     LFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRIKAVKNFLAVLSDSIC
                     SLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPPELFSQAYKLKNKEMN
                     AQLDYLNRYAT"
BASE COUNT         2104 a         2348 c         2406 g         2304 t
ORIGIN      
        1 atgcagcgcc ccgtctgact ttcactttac gagacgaaga gaggttgatg atgaaaattg
       61 gcgtattcgt acctattggc aacaacggct ggctcatttc gacccacgcg ccgcagtaca
      121 tgccgacctt tgaactgaat aaagccattg cgcaaaaagc ggagcattac catttcgatt
      181 tcgccctgtc gatgatcaaa ctgcgtggct ttggcggcaa aactgagttc tgggatcaca
      241 accttgagtc gttcaccttg atggcgggtc tggcagccgt gacctcgcgc attcagattt
      301 acgccactgc tgccacctta acgttacctc cggcaatcgt cgcccgtatg gccgcaacca
      361 tcgactccat ctctggcggg cgttttggcg tcaacctcgt gactggctgg caaaagcccg
      421 agtatgagca gatgggtatc tggcctggcg atgactattt ctcccgtcgt tacgactatc
      481 tcaccgaata tgttcaggtg ctgcgcgacc tgtggggcac ggggaaaagc gattttaaag
      541 gcgatttttt caccatgaat gattgtcgcg tcagtccgca accgagtatc cccatgaaag
      601 tgatctgcgc cgggcaaagc gacgctggca tggcgttctc cgcccagtat gccgatttca
      661 acttctgttt cggcaaaggc gtaaatacac ccacggcttt cgccccgacc gctgcgcgga
      721 tgaaacaggc cacagagcaa accggacgcg acgttggctc ttatgtgttg tttatggtga
      781 ttgccgatga aaccgacgat gccactcgcg ccaaatggga acactacaaa gcgggcgcgg
      841 atgaagaggc gttaagctgg ctaaccgaac aaagtcagaa agatacccgc tccggtactg
      901 acaccaacgt tcgtcagatg gccgatccca cttcggcggt aaacatcaat atggggacgt
      961 tagtcggttc ttacgccagt gtcgcgcgca tgttagatga agtcgcaagc gtgcctggtg
     1021 cagaaggcgt gctgttgacc ttcgatgatt ttctgtcggg aatcgaaacc ttcggcgagc
     1081 gcattcaacc actgatgcag tgccgcgccc atctctctgc gctgactcag gaggtggcat
     1141 gatgacaacc ttaaccgctc gaccggaagc cattaccttc gatccgcagc aaagtgcgca
     1201 gatcgtggtg gatatgcaaa acgcttatgc cacgccaggc ggctacttag atctcgccgg
     1261 gtttgatgtc tcaaccactc gcccggtcat tgccaacatt caaaccgctg tgaccgcagc
     1321 gcgagcggca gggatgttga tcatctggtt tcaaaatggc tgggatgaac agtatgtcga
     1381 ggctggcggc cccggctcac cgaattttca taaatcgaac gccctgaaaa ccatgcgtaa
     1441 gcagccgcag ctgcagggga aattgctggc gaaaggctcc tgggattatc aactggtgga
     1501 tgaactggtg ccgcagcctg gcgatattgt gctgccgaag ccgcgctaca gcggtttctt
     1561 caatacgccg ctggacagca ttttacgcag ccgtggaata cgccatctgg ttttcaccgg
     1621 tatcgctacc aacgtctgcg tcgaatcgac gctacgcgat ggcttttttc tggagcattt
     1681 cggcgtggtg ctggaagacg caacacacca ggcggggccg gaatttgcac agaaagccgc
     1741 gttgttcaat atcgaaacct tttttggctg ggtcagcgac gtcgaaacgt tctgcgacgc
     1801 gctttctccc acgtcctttg ctcgtatcgc ttaaggagtt tagcgatgcc aaaatccgta
     1861 attattcccg ctggcagcag cgcaccgctg gcccccttcg ttcccggcac gctggctgat
     1921 ggcgtggtgt atgtctccgg tacgctggct tttgatcaac ataataacgt gctgtttgcc
     1981 gatgacccaa aggcgcaaac ccgccacgtt ctggaaacta tccgcaaggt gatcgagacg
     2041 gcgggtggca cgatggcgga tgtgaccttc aacagcatct ttattaccga ctggaaaaat
     2101 tacgccgcga ttaacgaaat ctacgccgag ttttttccgg gtgataaacc ggcgcgattc
     2161 tacattcagt gcggactggt aaaacctgac gcgctggtgg aaatcgccac aattgcgcat
     2221 atcgccaagt gaggccgcga tgaaactttc actctcatct cccccttatg ctgatgcacc
     2281 cgtagtggtg ttgatttcgg gtcttggcgg tagcggcagt tactggttac cgcaactggc
     2341 ggtgctggag caggagtatc aggtagtctg ttacgaccag cgcggcaccg gcaataatcc
     2401 cgacacactg gcagaagatt acagtatcgc ccagatggca gcggaactgc atcaggcgct
     2461 ggtagccgca gggattgagc gttacgcggt ggtcggccat gcgctcggtg cgctggtggg
     2521 gatgcagctg gcgctggatt atcccgcgtc ggtaactatg ctggtcagcg ttaacggctg
     2581 gctacgaata aacgcccata cgcgccgctg ttttcaggtt cgcgaacgat tactgtatag
     2641 cggcggcgcg caagcatggg tggaagcgca gccgttgttc ctctatcccg ccgactggat
     2701 ggcggcccgc gcacctcgcc tggaggcaga agacgcgctg gcactggcgc attttcaggg
     2761 caaaaataat ttactacgtc gacttaacgc cctcaaacgc gctgacttta gtcaccatgc
     2821 ggatcgcatc cgctgcccgg tgcaaatcat ctgcgccagt gatgatctgc tggtgccaac
     2881 agcatgttcc agtgaacttc atgccgccct gcccgatagc cagaaaatgg tgatgcccta
     2941 tggcggacac gcctgcaacg tgaccgatcc cgaaacgttt aatgctctgt tactcaacgg
     3001 gcttgccagc ctgttacatc accgtgaagc cgccctgtaa ggaattgcta tgaacgaagc
     3061 cgttagccca ggtgcgctta gcaccctgtt caccgatgcc cgcactcaca acggctggcg
     3121 ggagacaccc gtcagcgatg agacgttaca ggagctttat gccctgatga aatgggggcc
     3181 gacatcagct aactgttctc cggcacggat cgtgtttatc cgcactgcag aaggaaaaga
     3241 acgtctgcgc ccggcgcttt ccagcggcaa tctgcaaaaa accctgaccg cgcccgtcac
     3301 cgctatcgtc gcctgggaca gtgaatttta tgaacggtta ccactactgt ttccccacgg
     3361 tgatgcccgc agttggttta cctccagccc acaacttgcc gaagaaacag cgtttcgcaa
     3421 cagttccatg caggcggctt atctgatcgt cgcttgccgt gcgctgggac tggataccgg
     3481 cccgatgtcg ggttttgacc gtcaacacgt tgacgacgcc ttttttgcgg gcagcacgct
     3541 gaagagcaat ctgctgatta atatcggcta tggcgatagc agcaaacttt ttgcgcgcct
     3601 gccacgtctg tcctttgaag aagcctgcgg gctgttgtaa ggagcaaact atgaacatag
     3661 ttgatcaaca aacctttcgc gatgcgatgt cctgtatggg cgcggcggtc aatatcatca
     3721 ccacggacgg tccagccggg cgcgccgggt tcaccgccag cgccgtctgc agcgtgaccg
     3781 acacgccgcc cacattactg gtgtgcctga atcgtggggc gtccgtctgg ccggtattca
     3841 atgaaaatcg aacgctgtgt gtaaatacac tgagcgccgg gcaggagccg ctttcaaatc
     3901 tttttggcgg caaaacgccc atggaacacc gctttgccgc cgcccgctgg cagacgggtg
     3961 tgaccggatg cccgcaactg gaagaggctc tggtttcgtt tgactgccgt atcagccagg
     4021 tggtgagcgt cggcacccac gacattctgt tttgcgccat cgaagcgatt catcgtcaca
     4081 ccacacccta cgggctggtg tggttcgatc gcagttatca cgcgctgatg cgccctgctt
     4141 gttaacctcc ttaaggagac agctgatggc aatgttcggt tttcctcact ggcagttaaa
     4201 atcgacctct acagagagcg gcgtggtcgc gccggatgaa cgattgccgt ttgctcagac
     4261 ggcagtaatg ggcgttcaac acgcggtggc gatgtttggc gcgacggtgt taatgccgat
     4321 tctgatgggg ctggatccca atctttccat tttaatgtcg gggatcggca cgttactgtt
     4381 tttctttatt accggcgggc gcgtccccag ttatctcggc tccagcgcgg cttttgtcgg
     4441 cgtggtgatt gccgcgacgg gctttaacgg tcagggcatt aacccgaata tcagcatcgc
     4501 gctgggcggg attatcgcct gtgggctggt ttataccgtc attggcctgg tggtgatgaa
     4561 aattggtaca cgctggattg aacgactaat gccgccggtg gtgacgggtg cggtggtgat
     4621 ggcgattggc ctgaacttag cgcctatcgc agtgaaaagt gtatcggctt cggcctttga
     4681 tagctggatg gcggtaatga cggtgctgtg tattgggctg gtggcggtgt ttacccgtgg
     4741 catgatccag cggttattga ttctcgtagg attaattgtc gcctgtctgc tgtatggcgt
     4801 gatgaccaac gttttagggc tgggaaaagc cgtcgatttt actctcgtca gctatgctgc
     4861 ctggttcggt ctgccgcatt tttccactcc tggttttaat ggccaagcga tgatgttgat
     4921 tgcgcccgtg gcggtgattc tggtggcgga aaacttaggt catctcaagg cggtcgccgg
     4981 gatgaccgga cgcaatatgg acccgtatat ggggcgagca ttcgtcggtg atggtctggc
     5041 aacgatgctt tccggctctg tcggcggtag cggcgtcacc acctatgctg aaaatatcgg
     5101 tgtgatggcc gtgacaaaag tctactcgac gctggtgttt gttgcagctg ccgtcatcgc
     5161 tatgctgttg ggcttttcgc cgaaatttgg cgcgctgatc cataccattc cggccgcggt
     5221 gattggtggt gcatcaattg ttgtgttcgg gctgattgcc gtcgcaggcg caaggatatg
     5281 ggtacaaaac cgtgtcgatt taagccagaa cgctaatttg attatggtcg cagtaacctt
     5341 cgtgctgggc gcgggtgatt ttgctctcac gcttggcggt tttacgttgg gagggatcgg
     5401 tacagcaacc tttggcgcga ttttactcaa tgcgttatta agcagaaggt tggttgacgt
     5461 tccaccgcca gaagttgttc atcaggagcc ataaacagaa aaccgccggg ttaccggcgg
     5521 tgatgacttt ttgacgagct taagaaaaaa atgcattcgt cattgattag cccggctagt
     5581 tgtcgctttt gccgtgactg ctcttgccac cttttttacc tgcttctgat gctctttcgc
     5641 ggtcttctgc aaaattgccg gaaccgcctc gatggtttgc cattactgac ctccgtctgg
     5701 gtatagacat catattgcat agaattaacg cggcgtatgg cggctgccat attttcgcta
     5761 cgtgggacta agcttagtga actgacgcgg acagacccgc gtttggtaat atcctgcaac
     5821 agcttcttag cgagaataga tatcatctcc aataattatc cataagccgc tcttatgatt
     5881 aaaaaaacgc gatgagataa aaatgtgtca ttttgcgaca aaattacgtg cttgtgaaag
     5941 ttgttataaa tcaaataagt ggttgtgaaa tttgcactct gaaaaggacg tcttatcttt
     6001 aaataagtgg tagcgaatcg ctacggaata gagataacac gaggagtggt tagaaatggc
     6061 taaagttctg gtgctttatt attccatgta cggacatatt gaaacgatgg cacgcgcagt
     6121 cgctgagggt gcaagcaaag tggatggcgc agaagttgtc gttaagcgtg taccggaaac
     6181 catgccgccg caattatttg aaaaagcagg cggtaaaacg caaactgcac cggttgcaac
     6241 cccgcaagaa ctggccgatt acgacgccat tatttttggt acacctaccc gctttggcaa
     6301 catgtccggt caaatgcgta ccttcctcga ccagacgggc ggcctgtggg cttccggcgc
     6361 actatacgga aaactggcga gcgtctttag ttccaccggt actggcggcg gtcaggaaca
     6421 aaccatcaca tccacctgga cgacccttgc gcatcacggc atggtgattg tccccattgg
     6481 ctacgcagcg caggaattat ttgacgtttc acaggttcgc ggcggtacgc cgtacggcgc
     6541 aaccaccatc gcaggcggtg acggctcacg ccagccaagc caggaagaac tgtctattgc
     6601 tcgttatcaa ggggaatatg tcgcaggtct ggcagttaaa cttaacggct aatcttcaac
     6661 aggaggatac gcatgccaac tcaagaagcg aaagctcatc acgtcggtga atgggcatct
     6721 ctgcgcaata cgtcgccgga aatagccgag gccattttcg aagtcgccgg gtacgacgaa
     6781 aaaatggcag aaaagatttg ggaagaaggt agtgatgaag tgttagtcaa agcctttgct
     6841 aagaccgata aagattcgct tttttggggc gaacagacca tcgaacgtaa aaacgtttaa
     6901 ccagcgactc ccccgcttct cgcgggggag ttttctgtta tttcaccgcc tcattcaaca
     6961 cgctatcaaa cttatccatc gggcagaaac cgttggcatc tatcgggcaa ccgcttaatt
     7021 ccagcgtcac gcgctgcgca ggtgcctgca gggttaacgc atcggcatta cgtaactgtt
     7081 ccgcactctg gtacacatat tcaattttca tcaaatcgcg attggctttg ctgtcatgcc
     7141 aacgctggaa aacgattttg ccgccaatcg gcgtgcgttc gttctggtca tgcaactgat
     7201 acggtttgaa atccagcgcc gttaacagag aggctatgtt ggagtcgtgc ccaactaaca
     7261 ccgtgatttt cggagcactg gcgcgatcgg tgaccagggc tttgtcgata taactgacca
     7321 gcggtttcgc aacattgcgc gccacttccg gtgaggtaaa cagactgtcc tggtaaccgt
     7381 ttttcagctt cgacaatacc ttccactgct ggtcagattt gatttctccc caggccacct
     7441 gatccatcgg aaaaccttcg tagtattgca aagtaaacgc atctaccagc gagttgccga
     7501 ctttcagcgg cccggaaaca cctggttctt gttgatactt ggcgctaaag gtatttttgc
     7561 catccaccag cgaacactgt tgtttctctt tacaggcagg ggaatcttta tagttaacga
     7621 ttttttccag tagctggtag ctgtcggtaa gctggagttt gctgagctct ttctccattg
     7681 ccgccaccgc ctgttcactg aatgcggcgg aatcatcggt gatcatcggg ttaaaggttg
     7741 ggtccatagt gcccattttt tcctggtgat gcacaggaat atcacaccca gggaatgcac
     7801 cagtaataaa gaactgggcg gtagcgacgg tacgttgcag actattggca taggcataaa
     7861 cggtgtccgg cggcgggcat tcccccgatt tcactatccc ctgctctgcc agccattcac
     7921 gcatgtaatg gcccatatac acttcgagca cgccaccttt ggtggtgagt tgcccaccgg
     7981 ggacgtccca ttcgggccat ttattcggcg tcgactgctc cagcacactg ccattgttcg
     8041 ccagcggcgc acgtaagtta tggcggctca tcatgagcac ttgctgtagc tgatagcctt
     8101 ccggtacggt ttgtgcctga gcatttgaag cgagtaaaac tatccctgcc acagttgcgg
     8161 cgattagcgt tttgttcatt cctggcacct cttttgttat caatcattaa agagtgtgac
     8221 agaaatatga cttttttccg ggaacaggtt cgcaaaatgg cttgttgaac gcctgttttt
     8281 ctatatacat cttcaaaaac aggcaaggtt tgacgcaaac tatgcctgat tagcccctgc
     8341 ctcgcgtacg ccgctgataa ttgcacctgt ggctaaacag aatatctgcg gcgttaccaa
     8401 tacagagaca atatttacca ataaaaaagt gtgagactaa taacaatggt ttttagcata
     8461 tgcctttgcc attaagttgc ataacgattt aaatagtcaa gttgcgcatt catttcctta
     8521 ttttttaact tataagcctg agaaaaaagt tcaggaggga ggtaaacatc ataatgattg
     8581 ccagtgtttg ttaaatatcc atactcaaga tcttcatctg ttgaaaagga ttcatttccc
     8641 aaggagattg atgccacatt actattaaca ataaataatg agcaaatgct atctgaaaga
     8701 acagcaagaa aattcttcac cgctttaatc ctatccgacg tgtcggtatc taaagcaagc
     8761 aaattcttgt tatcattaaa tataaactcg ggtaaatgca gtatgcattt tttgtcattc
     8821 aagcaactat caatgaattg ttttttaaaa aacagaaagc tccatgacat aaaactaccc
     8881 acaccctgaa ttttgttgtc agattgcaga tcaacgccaa ataatgtctt tacatcttcc
     8941 ctgagattat agaatgccgt atcaacaaga gcctcgatcg tttgtttatc aacaatttta
     9001 ataaactctt cgtatacttg cttttgcgtc ttatggctgc ccagcgccca tgaatactca
     9061 tgttctttta aaaccagtat ggctcttaac agacaatatc catcgccatc tactggagcg
     9121 tattttaatc catatgtttt attaatttca ctctgaataa aa
//
LOCUS       DAZXUK010000158         9108 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZXUK010000158 DAZXUK010000000
VERSION     DAZXUK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9108
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.158"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(41..943)
                     /locus_tag="ACH3L8_002863"
     CDS             complement(41..943)
                     /locus_tag="ACH3L8_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFT4085902.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(1081..2322)
                     /gene="yihS"
                     /locus_tag="ACH3L8_002864"
     CDS             complement(1081..2322)
                     /gene="yihS"
                     /locus_tag="ACH3L8_002864"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFT4085903.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDEKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(2339..3217)
                     /gene="yihT"
                     /locus_tag="ACH3L8_002865"
                     /pseudo
     CDS             complement(2339..3217)
                     /gene="yihT"
                     /locus_tag="ACH3L8_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
     gene            complement(3241..4137)
                     /gene="yihU"
                     /locus_tag="ACH3L8_002866"
     CDS             complement(3241..4137)
                     /gene="yihU"
                     /locus_tag="ACH3L8_002866"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFT4085904.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4305..5201
                     /gene="yihV"
                     /locus_tag="ACH3L8_002867"
     CDS             4305..5201
                     /gene="yihV"
                     /locus_tag="ACH3L8_002867"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFT4085905.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5235..6020
                     /locus_tag="ACH3L8_002868"
     CDS             5235..6020
                     /locus_tag="ACH3L8_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFT4085906.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6119..6718
                     /gene="yihX"
                     /locus_tag="ACH3L8_002869"
     CDS             6119..6718
                     /gene="yihX"
                     /locus_tag="ACH3L8_002869"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFT4085907.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            6712..7584
                     /locus_tag="ACH3L8_002870"
     CDS             6712..7584
                     /locus_tag="ACH3L8_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFT4085908.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            7581..8018
                     /gene="dtd"
                     /locus_tag="ACH3L8_002871"
     CDS             7581..8018
                     /gene="dtd"
                     /locus_tag="ACH3L8_002871"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFT4085909.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            8063..9004
                     /gene="fabY"
                     /locus_tag="ACH3L8_002872"
                     /gene_synonym="yiiD"
     CDS             8063..9004
                     /gene="fabY"
                     /locus_tag="ACH3L8_002872"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFT4085910.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2119 a         2307 c         2450 g         2232 t
ORIGIN      
        1 gaaatgttct tcaatgattt tgactgctaa tcaatacaag ttattttctc tgtccgtaaa
       61 tattaaaaaa caggcggtgc ggttttcctg gttccagtaa taataagtcg atacctgaat
      121 taaaggcgtt aggcgggcaa ctcataggtt caactgccag tccctgacgc tgtaattttt
      181 caccgctata aacctgcacc cagggttgat cggaacaaag gcttaccgaa agcgcctgct
      241 gtggatgggt aatggtcatt tcccacaaat cattagcggt tttaaaggtg tggtcgattt
      301 ttgtcgcggc tatttttttc gcctgcgtga aatttaaatc cagctcatca acgtggtgaa
      361 gcgttgtcgg atttacgtgt tcatcgacgg cataaacctg gtttgccgga agctggaaca
      421 gatattcatc aacagatgtt aagttgcagg ttaaataagg atgaataccc acgccataag
      481 gcgctgcgac agtgccgata ttctggcttg cgatctcaac ggaaagaccc gtgtgtgcgt
      541 tgagcgaata aacaacttgt gaagccagca taaacggata gccgtaactg ggcggcagaa
      601 aagctgtcag ggtaacgctg gtggtggtta attcactaat ttgccagtct cgccaggcaa
      661 gcagcccgtg aatagcggct tttgaactat gttcattaat gggtaactga tattcctgcc
      721 cctgataacg gtaacagccg ttggcgatcc ggttcggcca cggaataagc actttaccca
      781 gatgcgccag cggcatctct tcgggtttgt gcgggatgac taaatgacaa ccctgaaagg
      841 ttaattcagc cagaccagca ccgacggtca caatggtcgc gtgataatcc cccgcggcaa
      901 gtgaaacggt ctgtccactg caatgcatat tggtgatttg catacaaggc accttaatta
      961 acgacataca gcctcccgtg aggctgatgt caaacccaaa aactgcaccg gacggtcccc
     1021 tcgccccttt ggggagaggg ttagggtgag gggaaaaccc gcaccctatt ttcagatcag
     1081 ttatttcgca ttaatatcca gcagacccgc tgcaaccgct ggagccatgc cgggcgctaa
     1141 cgggatacgc gggatcacca ggcaatgcag caggtgataa atatcctgtt tgccgtccca
     1201 gactttggtg atgaccttat tgtccgcatc cagctcctgc caccaggaac cattttcata
     1261 gtccatcagg tacttaatgc agtactccca ccatgtttga taccaggttt catactggcg
     1321 atcgccagtg acggtgtaga gcgcgtaggc cgtacccatt gcttcgacga taggccaacg
     1381 tacacgttcg cggaccaccg gttttccttc ccagtcaacg gtataaacaa tcccgtccgc
     1441 tccatcgggt gcccaggcat cgcgcacggt ggcgttaaac agacctttgg catcttctag
     1501 cagccatgct ggtggttgtt cgcaacgggc ttccagggca gcatggatgt gcagcattaa
     1561 acggccccat tcgatccagt ggcctggtgt acctccgaac gcgcggaagc gatgcgccgg
     1621 gttatctttg ttgtaatccg gcagcggatt ccactgggta tcgaagtgtt cgttaacgcg
     1681 ataatgatta tttctggcga cgtcgtggat aatcacggaa gccacgcgaa tcgcgcgatc
     1741 cagccatttt ttgtcgtgag tgacgtcata aacaatcaag aaagcttcca ccgcgtgcat
     1801 attggcattg ccgccgcggt actcttcggt tttgctgaag gcttcgtccc aggactccag
     1861 gcacatctgc tcttcttcgc tccagaaata tttctcgata atctcaatgg tgtaatcgag
     1921 cagcttgcgc gcttccgggt gacccgttgt gacggcgctg gcggcaccca gcagagcaaa
     1981 gaagtgttga tagccctgtt tggaggcatc caccacgccc tcgtcattca cgcaggcata
     2041 ccagccgcca tatttttcat cacgcagtgc gccgttcatg gctttaatac cgtgatcaac
     2101 caacgcgtaa gcgccaggtc gacccatcgc cgcagcaacg gaataaacgt gcaacatacg
     2161 agctgtgatc cacagatggg tgcccatctc ttctttgatt tgccctttat tgcctaacca
     2221 gccaaaacca gtcggcacta cggaattttt accaaaatca aagatgcggt cggtttcctg
     2281 ttccagccaa cggttgtggc ttagggtgtt aaaccatttc attcctttta tcctcatctt
     2341 agcggcgttt agccatcatt tcgtcgacga tatcgccaag ttgttgtaat ttcggtgcgc
     2401 aaacgtcacg gagcatcagc tcgttgtctg gtaaaccgac gaccgatgcc cagactgcac
     2461 ggcctgccag gaatcccgat gcgcccgccg tcattgccac gcgtacggca cgcgggaaca
     2521 gtttttcgtc gacactggaa gagaggatca cccatggcat attgatatgg tcattcagac
     2581 gttgtgaagc acagagaagc tcttgttgcg gacctttgcc ataaagcggc atttcaactt
     2641 tgtagaggtc agcgccactg tcgcccagct ctttggcggc atcgatgatc gcttgttcgc
     2701 gatcgaattt atcgccacga cgcggtgggc ggacgactgg ctcaatgatg cttaccagac
     2761 cgtgtgagtg gcacagttcg ttgaactctt tcaccatatc cagacgttgc tgcgcatctt
     2821 catcactacg ctacagcacc agcagtttta aggctttgcc accgtcttgt ttgatctgta
     2881 gcggattgat tttgcggtca ataaccacgc tatcgaccgg aataccgttg ccaggaatga
     2941 actcatcggc ggcgacaatc atggcgcaac ttttggcaat cgcgttttgc tcaaccacct
     3001 ggcggtagca gaattgttga tctaccagaa tcgccgaggc ataaggcgag agggcctttg
     3061 cagcgttaac tttgaaatca gttaaaacgc tatcggctac cggagcgggt gccccagccg
     3121 cggcaaacat catgcgcatg gcttcgcgct gatcgaccgc cagcatggca aaaccgcccg
     3181 atgcgcgcgt aatgtcgttg atggtgtact tattcattca tttattcctt tatccagtcg
     3241 ttacattttt actttggcag tcatcccggc actgacacgg acctgttcca gaatggcgga
     3301 ccagtcctgg cgaccgcgac ccgctgcgcg cgcctggcta taaacctccc gtgaggcggc
     3361 ccccagcggc attggcacat gcagctggtt ggcgacatca agggcgatgc caagatcctt
     3421 atgggcaaga tcgatcatga aggcgggaga aagatctccg ctgaggactt tgtttggcca
     3481 ggaagtggtg aagtgacctt taccggcggc ggtaccgctc atcactttga cggcaacatc
     3541 gaagggaaga ttcagggctt cgcacaaaac ggcggcttct gccgaaagcg cattgagcgc
     3601 gatgctcatg tagttgttga tgagcttaac gcggatcccc atgcccggac cgccagcgtt
     3661 gatcaactca ctgcccatcg ccatcaggat cggtgtggca cgttcaactt gttcagcggt
     3721 gccgccagcc agcagtaaca gagtaccggt aatggcattt gcagaagtac ggcctaccgg
     3781 aacatccatc atgctgaagc ctttggcttg catatcggca atcaatttat cggtttgcag
     3841 cggatggatg gtggacatat caatgaccag cgcatcggta gataagcctt cgcaaacgcc
     3901 gttttcaccg aacaacacgt tgcgcaccag atcgccattc ggcaacatgg taatgataaa
     3961 ttcggcatct ttagcggcct gcgccgggtt ggcggcggga gtcgcacctt tgtctaccag
     4021 atgccgcaca gcttcggcgt tcacatcaaa gacgcgaagt tgatgccctt gctgcaataa
     4081 attgctcgcc attggcgaac ccatttgtcc taaaccgata aacgcgattg ctgccataac
     4141 cctctcctga atacagtgat gtcacttttt gtcatttatg acatgctttg cttgtctgtt
     4201 tttgatcgaa tttgtaattt atcgtcaaaa aattgacagc cgtcactttt taaacaattg
     4261 gtgaaattaa aatgaacgca tcccaaaatg tttaaggaat gaccatgatt cgtgttgctt
     4321 gtgtaggtat aaccgtgatg gatcgcatct attacgtgga agggttaccg acggagagcg
     4381 gtaaatacgt ggcgagaaat tatacggaag ttggtggcgg gccagcggcg acggcagcgg
     4441 ttgcggcggc aaggctgggg gcgcaggtcg attttattgg tcgcgtaggt gatgacgacg
     4501 cgggcaacag cctgctggca gagctggaat cctggggcgt taacacccgt tacaccaaac
     4561 ggtataacca ggcgaaatct tcgcaatccg ccatcatggt ggataccaaa ggcgagcgga
     4621 taatcattaa ctaccccagc ccggatctgc tgcctgacgc agagtggttg gaggaaattg
     4681 atttctctca gtgggatgtt gtgctggcag atgtacgctg gcacgacggc gctaaaaaag
     4741 ccttcaccct ggcccgtcag gcgggtgtga tgaccgttct ggacggggac attacgccgc
     4801 aggatatcag tgagctggtg gcattaagcg atcacgcggc cttttcagaa ccgggtctgg
     4861 cgcgcttaac gggcgtgaaa gagatggcca gtgcgctaaa acaggcacaa acgctcacaa
     4921 atggtcatgt ctatgtgacc cagggtagcg caggctgcga ctggctggaa aatggtgggc
     4981 gtcagcatca accggccttc aaagttgatg tggtagatac cacaggtgcg ggagatgttt
     5041 ttcacggcgc tttggcggtg gcgctggcaa caagtgggga tttagcggag tcagtccgct
     5101 tcgccagcgg tgtagcggcg ttaaaatgca cacgtcccgg tggacgagcc gggatccctg
     5161 actgtgatca aacccgatct tttttgtcac tttttgtata aaatgccagg gtgatggttt
     5221 ttcgaggaat tttcatgagc cttaccgaac taaccggtaa cccgcggcac gaccaactcc
     5281 tcatgctgat cgccgagcgt gggtatatga atattgatga gctggcaaat ctgctggatg
     5341 tctccacgca gacggtccgc cgggatattc gtaaattaag cgagcaaggc ctgattacgc
     5401 gccatcacgg tggcgcgggt cgggcttcca gcgtcgttaa tacggcgttc gagcagcgtg
     5461 aggtttcgca aaccgagaaa aaaaaagcga ttgccgaagc ggtggcagac tatattcctg
     5521 atggttcaac aatatttatc accattggta cgactgttga gcatgttgcc cgggcgttac
     5581 ttaaccataa tcatttgcgg ataatcacca acagcctgcg tgtggcgcat attctttacc
     5641 acaacccgcg ctttgaagtg atggtgcccg gcggtacgtt gcgctctcat aatagcggga
     5701 tcattggccc ttcagcggcg tcctttgtgg ctgattttcg tgctgattat ctggtaacaa
     5761 gcgttggggc gattgagagc gatggcgcgt tgatggagtt tgatgtaaac gaagctaacg
     5821 tggtgaaaac gatgatggcg cacgcgagaa atattctgct ggtcgccgat cacactaagt
     5881 atcatgcttc ggcggcggtt gaaattggta acgtggcaca ggtcactgcg ctctttaccg
     5941 acgagctgcc gcccgctgcg ctaaaatcac gcttacaaga cagccaaatt gaaatcattc
     6001 ttccccagga agacgcgtag atttttgtga ccgttaatct ggcttcatac cttgccacat
     6061 agccaaaccc atcctttccc gctacagtta atttcttgtg gcgcgaaagg aggcaaaaat
     6121 gctctatatc tttgatttag gtaatgtgat tgtcgatatc gactttaacc gtgtgctggg
     6181 agcctggagc gatttaacgc gtattccgct ggcatcgctt aagaagagtt ttcatatggg
     6241 ggaggcgttt catcagcatg agcgtgggga aattagcgac gaagcgttcg cagaggcgct
     6301 gtgtcatgag atggctctac cgctaagcta cgagcagttc tctcacggct ggcaggcggt
     6361 gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat aaactgcgtg agcaggggca
     6421 tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc accttctggc cggaagaata
     6481 cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg caagatctgg ggatgcgcaa
     6541 acctgaagca cgaatttacc agcatgtttt gcaggcggaa ggtttttcac ccagcgatac
     6601 ggtctttttc gacgataacg ccgataatat agaaggagct aatcagctag gcattaccag
     6661 tattctggtg aaagataaaa ccaccatccc ggactatttc gcgaaggtgt tatgctaaaa
     6721 accattcagg acaaagccag gcaccgtacc cgtccactat gggcctggct aaaactactc
     6781 tggcaacgta ttgatgagga caacatgaca accctggcag gtaaccttgc ctatgtgtcg
     6841 ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc ttttcgccgc ttttcccatg
     6901 ttttccgacg tcagcattca gttacgtcac tttatttttg ccaactttct gcctgctact
     6961 ggcgatgtta ttcagcggta tatcgaacaa tttgttgcta attccaacaa gatgaccgcc
     7021 gttggggcgt gcgggctgat cgtcaccgcg ttattgttga tgtactccat cgatagcgcg
     7081 ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa tttactcgtt cgccgtgtac
     7141 tggatgattt taacgctggg gccgctgctg gcaggggcca gtctggcgat cagttcctat
     7201 ttgctctccc tgcgctgggc gagcgatctc aatactgtca tcgacaacgt gctgcgtatt
     7261 tttccgttgc tgttgtcgtg gatctccttc tggttgctgt acagcattgt tcctaccatc
     7321 cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg ccgcactcct gttcgaagca
     7381 ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt catatcagct catttacggt
     7441 gtgctggcgg tgatccccat tctctttgtt tgggtctact ggacatggtg tatcgtcttg
     7501 cttggcgcgg aaattactgt cactctcggg gaataccgca aactaaaaca agcagctgaa
     7561 caagaagaag acgacgaacc atgattgcat taattcaacg cgtaacccgt gccagcgtca
     7621 ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact tttggtgtta ttgggtgtcg
     7681 aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga gcgtgtgctc ggctaccgca
     7741 tctttagcga tgccgaaggc aagatgaatc tcaacgtgca acaggcgggc ggcagtgtac
     7801 tggtggtttc ccagtttacc ctcgccgcag ataccgaacg ggggatgcgc ccaagtttct
     7861 ccaaaggcgc atcaccggat cgcgcagagg cgttatatga ctatttcgtc gaacgctgcc
     7921 gccagcaaga gatgaacacg caaacaggac gcttcgctgc cgatatgcag gtatcgctgg
     7981 tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc cagcttccag ggttgtcacg
     8041 ggaaacaaga gagagtatcg ctatgtatca ccttcaggtt ccacaaacag aagaagagtt
     8101 agagcgttac tatcactttc gctgggaaat gttgcgtaag cccctgcatc aaccaaaagg
     8161 ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag atggtcgtcg acgagcaggg
     8221 taatctggtg gcggtaggcc gattgtatat taatgccgac aatgaagcgt ccattcgctt
     8281 tatggtcgtt cacccggacg tgcaggacaa agggttagga acgctgatgg cgatgaccct
     8341 ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc tgtagcgccc gtgaagacgc
     8401 ggtggagttt ttcgccaagc tggggtttgt taatcaggga gaaatcacca cgccaaccac
     8461 cacgccgatt cgccattttt tgatgattaa gcccgtcgcc actctggatg atattttgca
     8521 tcgcggcgac tggtgcgcgc agctgcaaca ggcgtggtac gaacatatcc cgcttagtga
     8581 aaaaatgggc gtgcgtattc aacaatatac cgggcaaaaa tttatcacca ccatgccgga
     8641 aaccggcaat cagaatccgc accatacgct gtttgccggg agtttattct cgctggcgac
     8701 gctcaccggt tgggggctta tctggctgat gctgcgtgaa cgccacctcg gcggaacgat
     8761 tattctggcg gatgcgcata tccgctacag caaaccgatt agcggtaaac cccatgcggt
     8821 agccgacctt ggtgccttaa gcggcgatct cgaccgtctg gcgcgcggac gaaaagcacg
     8881 ggtgcagatg caggtcgaaa tctttggcga cgagacgccg ggtgcagtgt ttgaaggcac
     8941 gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa gagggcggga acgaagaaga
     9001 gtagcttatg gagaggggcg gtgctgcctc tctcattcat tccggtcatt tacatcagtc
     9061 cctgactatt ccggttatat tgaataagaa ctttataaat ttttattc
//
LOCUS       DAZXUK010000159         9071 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZXUK010000159 DAZXUK010000000
VERSION     DAZXUK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.159"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="ACH3L8_002873"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="ACH3L8_002873"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFT4085911.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="ACH3L8_002874"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="ACH3L8_002874"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFT4085912.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACH3L8_002875"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACH3L8_002875"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFT4085913.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACH3L8_002876"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACH3L8_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFT4085914.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACH3L8_002877"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACH3L8_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFT4085915.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACH3L8_002878"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACH3L8_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFT4085916.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="ACH3L8_002879"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="ACH3L8_002879"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFT4085917.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="ACH3L8_002880"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="ACH3L8_002880"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFT4085918.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="ACH3L8_002881"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="ACH3L8_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFT4085919.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACH3L8_002882"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACH3L8_002882"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFT4085920.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACH3L8_002883"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACH3L8_002883"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFT4085921.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..>9071
                     /locus_tag="ACH3L8_002884"
     CDS             8212..>9071
                     /locus_tag="ACH3L8_002884"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFT4085922.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNI"
BASE COUNT         2132 a         2461 c         2293 g         2185 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg a
//
LOCUS       DAZXUK010000160         8960 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZXUK010000160 DAZXUK010000000
VERSION     DAZXUK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8960
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.160"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1060
                     /gene="ompN"
                     /locus_tag="ACH3L8_002885"
                     /pseudo
     CDS             1..1060
                     /gene="ompN"
                     /locus_tag="ACH3L8_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpN"
     gene            1201..1635
                     /gene="uspF"
                     /locus_tag="ACH3L8_002886"
     CDS             1201..1635
                     /gene="uspF"
                     /locus_tag="ACH3L8_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFT4085923.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1811..2746
                     /gene="ttcA"
                     /locus_tag="ACH3L8_002887"
     CDS             1811..2746
                     /gene="ttcA"
                     /locus_tag="ACH3L8_002887"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFT4085924.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2875..4242)
                     /gene="dbpA"
                     /locus_tag="ACH3L8_002888"
     CDS             complement(2875..4242)
                     /gene="dbpA"
                     /locus_tag="ACH3L8_002888"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFT4085925.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(4272..4445)
                     /gene="ynaL"
                     /locus_tag="ACH3L8_002889"
     CDS             complement(4272..4445)
                     /gene="ynaL"
                     /locus_tag="ACH3L8_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFT4085926.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4720..5703)
                     /gene="zntB"
                     /locus_tag="ACH3L8_002890"
     CDS             complement(4720..5703)
                     /gene="zntB"
                     /locus_tag="ACH3L8_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFT4085927.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5717..5764)
                     /gene="ydaN"
                     /locus_tag="ACH3L8_002891"
     CDS             complement(5717..5764)
                     /gene="ydaN"
                     /locus_tag="ACH3L8_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFT4085928.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            5958..7190
                     /gene="dgcM"
                     /locus_tag="ACH3L8_002892"
                     /pseudo
     CDS             5958..7190
                     /gene="dgcM"
                     /locus_tag="ACH3L8_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(7211..7774)
                     /gene="smrA"
                     /locus_tag="ACH3L8_002893"
                     /pseudo
     CDS             complement(7211..7774)
                     /gene="smrA"
                     /locus_tag="ACH3L8_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(8028..>8960)
                     /locus_tag="ACH3L8_002894"
     CDS             complement(8028..>8960)
                     /locus_tag="ACH3L8_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4085929.1"
                     /translation="LVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
BASE COUNT         2447 a         2231 c         2053 g         2229 t
ORIGIN      
        1 tttataataa agacggcaac aaattagatc tgtatggcaa agttgatggc ctgcattatt
       61 tttctgataa ttcagcgaaa gatggcgacc agagctatgc gcgtctgggt tttaaaggcg
      121 aaacccaaat taacgatcaa cttactggct acggtcaatg ggaatacaat attcaggcaa
      181 acaacactga atcttcaaaa aaccagtcat ggacccgtct ggcatttgcc ggactgaaat
      241 ttgcagatta cggttctttc gattacggac gtaattatgg cgtaatgtac gacatcgaag
      301 gctggaccga tatgctgcct gaatttggcg gtgactctta taccaatgca gacaacttta
      361 tgactggtcg agccaatggc gtcgcgactt atcgtaatac tgatttcttc ggtctggtaa
      421 atggtctgaa cttcgcggtg cagtatcaag gtaacaacga aggagccagt aatggtcagg
      481 aaggcaccaa taacgaacgc gatgttcgtc atgaaaacgg tgacggctgg ggtctttcca
      541 caacatatga tttaggcatg ggctttagcg ctggtgcggc atacacctct tctgaccgca
      601 ccaatgacca ggttaaccat actgcggcgg gtggtgataa agcagacgcg tggactgctg
      661 ggctaaaata cgatgctaac aatatttacc tagcaaccat gtattcagaa acgcgtaata
      721 tgaccccgtt tggcgacagc gattatgcag ttgcgaacaa aacccagaac tttgaagtta
      781 ctagtaccag tttgattttt gtctgcgtcc ggcagtctct ttcctgatgt ctaaaggccg
      841 tgaccttcac gctgctggtg gtgcagacaa cccggcaggt gttgatgata aagatctggt
      901 taaatacgcc gatgttggcg cgacttacta tttcaataaa aacatgtcca cctatgttga
      961 ctataaaatc aacctgttgg atgaagatga cagtttctac gctgccaacg gcatctctac
     1021 cgatgatatt gtcgctttag gtctggttta tcagttctaa atcctcctgc ccgctgttat
     1081 ggcgggcttt ttctgcttat tcttcctcct ttgatctaaa ttaaaaatgt gaactccgtc
     1141 attacacaaa aagtgtcatc tggcgttaca ctttatgcgg atactaaaac aggaggtttt
     1201 atgaacagaa cgattcttgt ccctatcgat atttcagatt cagaattaac tcaacgcgtg
     1261 attagccacg ttgaggcaga ggcaaagatt gatgatgcag aggttcattt cctgacggta
     1321 ataccttcac tgccctacta tgcctctctg ggtttagcgt attccgcaga attaccggca
     1381 atggatgacc tgaaagcgga agccaaatcg caactggaag agatcattaa aaaatttaaa
     1441 ctgccaaccg acagagtgca tgtccatgtt gaggaaggct cgcccaaaga ccgcattctg
     1501 gaattggcga agaagatccc cgctcatatg atcatcattg cttcccatcg accggatatc
     1561 accacttatc tgctcggttc caacgccgca gctgtagtgc gtcacgcaga gtgctccgtg
     1621 ctggttgtgc gctgacacta acgcccgcac attgctgcgg gctttttgat tcatttcgca
     1681 aatgtgctga cattttcccc tctaatccgt accatacacg ccacagtttt tatatcagat
     1741 ttcttatgct gagcgttccg gcatgacgca tactcttctg atgccatata acgaattgag
     1801 tcgcttttaa atgtcgcaaa atcaagaaat tagtaagaaa gaacaataca acctgaacaa
     1861 attacaaaaa cgtctgcgtc gtaacgtggg cgaagccatt gctgacttca atatgattga
     1921 agaaggcgat cgcatcatgg tttgcctctc cgggggtaaa gacagctata ccatgctgga
     1981 gattctgcgc aatttgcagc aaagcgcgcc aatcaatttt tcgctggtgg ctgttaacct
     2041 cgatcaaaag caaccgggct tcccggaaca cgttctgccc gagtatcttg aaaagctggg
     2101 cgttgagtac aagattgttg aagagaacac ttacggtatc gtgaaagaga agattccaga
     2161 gggcaaaacc acttgctcac tgtgttctcg gctacgtcgc ggtatccttt atcgcaccgc
     2221 aacggaactg gggacgacga agatcgcgtt gggtcaccat cgtgacgata tcctgcaaac
     2281 gttgttctta aatatgttct acggcggcaa gatgaaaggt atgcctccga aactgatgag
     2341 cgatgatggc aaacatatcg ttattcgtcc actggcctac tgccgcgaga aagatattca
     2401 gcgatttgcc gatgcaaaag cgttcccgat tattccgtgc aacctgtgcg gttcacagcc
     2461 taacctgcaa cgtcaggtga ttgctgacat gttgcgtgac tgggataaac gttatccagg
     2521 gcgtatcgag acgatgttca gcgcgatgca gaatgtggtg ccgtcgcatc tgtgcgatac
     2581 caacctgttc gatttcaaag gcattaccca cggttctgaa gtggttaacg ggggtgatct
     2641 ggcgtttgat cgcgaagaga tcccactaca accggcgggc tggcagccag aagaagatga
     2701 aaatcagttg gatgagttac ggctgaatgt ggttgaagtg aaataaccag gatagcgccc
     2761 gatgcgcaag cgtatcgggc tactcttatg gaggccggat aagacgcggc cagcgtcgca
     2821 tctggcaatc ccgaataaga tgtttactct tgcacccggc aattcaacat ttcattattt
     2881 taataaccgc acccggcacg tttttccttt aatcttcccg ccctgtaact gtttccatgc
     2941 tttatgagca acagcctgac ggaccgcgac atagacatgc gccggatgca cggcgatttt
     3001 gccaatatct gcgccatcaa gcccgatatc tcctgtcagt gcacctaata catcacccgg
     3061 gcgcattttg gcttttttcc cgccatcgat acacaacgtt gccatttctg cttccagcgt
     3121 cacaatggaa ctattagctg gcggcgtttg ccagttaagt tttatctgca acatgtcaga
     3181 aatgatattg gcccgctgtg cttcttccgg agcacagaaa ctgatcgcca gaccgctatt
     3241 tcctgcacga gctgtacgac cgatgcgatg tacatgaact tcagggtccc acgccagctc
     3301 aaagttcacc accagctcaa gcgatttaat atccagacca cgcgcagcaa catcagtcgc
     3361 aaccagtaca cgggcgctac cgttagcaaa acgtaccagg gtctgatcgc gttgctccag
     3421 atcgccgtgt aacgacaatg cactttgctc tacttcattc agcgcgtcgc agacagcctg
     3481 gcaatctttt ttggtattgc aaaacaccac acaagaggat ggctgatgca agcttaataa
     3541 ccgttgcaac agaggaattt tgcctttact ggatgtctca taaaattgtt gttcaatggg
     3601 tggcaaagca tctgttgagt caatttcaat cgccaaagga tcgcgttgca ctcgtccgct
     3661 gattgcggcg atggcttccg gccaggttgc cgaaaacaga agcgtctgtc gagatgcagg
     3721 cgcaaaacgg atgacatcat caatggcatc gctaaatccc atatccagca tgcggtcggc
     3781 ctcatccatc accaaagtat tcagcgcatc cagtgatacc gtgccttttt gcaagtgatc
     3841 cagcaaacga cccggcgttg ccacgataat atgcggcgca tgttgcagcg aatcacgctg
     3901 cataccgaac ggttgaccac cgcacaacgt caaaattttg gtatttggca gaaaacgcgc
     3961 cagccgacgc aattcacctg ccacctgatc cgccagttca cgcgtaggac acagcactaa
     4021 agcctgggtt tgaaatagcg acgcatcaat ttgctgtaac aagccgaggc caaaagccgc
     4081 cgttttgccg ctgccggttt tcgcctgcac gcgaacatct tttccggcaa ggatcgccgg
     4141 aagcgcggcg gcctgcaccg gcgtcatggt taaataaccc aactcattaa ggttcgtgag
     4201 ttgggcggga ggcaaaacat tcagggtaga aaaagcggtc acaatctatt ctcgtggtca
     4261 tcgacgcaaa gttagcaggc gcgtatcctc gcagatctac gctcacgatg cgacaattta
     4321 atcggttctt catcgggtgg tgggtcaggc atgggttgcg ggcgagggat cggatcgggc
     4381 actggaacag gatcgccagg aatcggttca gggacaggaa tttgcaaata aataagtgtc
     4441 gtcatatttc cctccggtca ttgggtggac tcttaaaggg tagacgctga taaataacag
     4501 gcaaaaaaaa gccgactcat caaagtcggc gtcgtacgaa tcaattgtgc tatgcagtaa
     4561 ttcaaaaaag gaagtaagac aatatggagc gcaacgccca tcgcttgacg ttgcattcac
     4621 ctgcaaggga gatattgccc tgaatgggta gagagtttat tgacttcgct caaactttgc
     4681 ggcgtttttg tatacagaca gccggaaaaa ttgcttttgt tacaaccatt tactacggtg
     4741 caaccataaa gcaacaccac caataagaac aactaacaga atacaaaaaa ttgaaaatcc
     4801 gaattgccac ccgccgccag ggatcccacc aaggttgacg ccaaataacc cggtcagaaa
     4861 ggtactgggt aaaaagacca ttgccatcaa cgacattgta taggtacgac gagctaaatt
     4921 ttcctgcatc acctgagcga tttcatccgc catcacgcca gtccgtgcta tacaggcgtc
     4981 gatttcgtca aggccgcgcc caaggcgatc ggcaatatcc tgcatccgac ggcgttggtc
     5041 atcgctcatc cacggcaaac gttcactggc aagacgagca taaacatcac gttgcggtgc
     5101 catatagcga cgcatcacaa ttaattgttt gcgcagcaga gccaggaatc cacgcggtgg
     5161 aatttgctga tcaaggagat tatcttcaag gtcgataatt ttatcgtgca gctgctcgat
     5221 aaattcactg gaatgatcgg tcaacgcatc gcacacatcc accagccatc ccccgcaatc
     5281 ggtcggaccc gtgccctctt ccagatcgct caccacatcg tccagcgcca gcactttgcg
     5341 ttgtcgggtc gaaacaatta accgcccgtc catatataca cgcatggcga ccagttgatc
     5401 ggggcgttca tcggtgctgc cgtttataca gcgcaatgta atcagcgtgc cttcaccgaa
     5461 acggctgact cggggccgtg tgctctcgcc cgccagcgca tcacgtacgt tattgggaag
     5521 cagcggtgtt gtcgccagcc attgggcgct atcatggtgt acataattaa ggtggagcca
     5581 gcagggatgc gcttcatcaa tcacatctgt attttccagc ggtttaacgc cgcctctacc
     5641 atccagcatc caggcaaata ctgcatccgg gacattaacg tccgatccct taatcgcttc
     5701 cactgtgcct ccatcatcaa cgcattattt tgtagtctag ccttctggcc ctgttacgca
     5761 acatctcatc accccattac cctgaaatga ttaataaaat tctgtctaaa ttgaatacaa
     5821 aaagcaaaat gcttttccgt atacaaaccg tgtgaagtgt taaatagcgt ctatcattat
     5881 cagaattatc tgatcatatg acgtggcttt tttgcgatcg gatagcaaca aaaattgata
     5941 aaaataacgg gatctcaatg attacgcaca acttcaatac cctggactta ctcaccagtc
     6001 ctgtctggat tgtttcgccc tttgaggaac agttaattta tgccaatagc gcggcgcgac
     6061 tgttgatgca agacctcacg tttagtcagc tacgaaccgg accctattcc gtctcctcac
     6121 aaaaagaact gccgaaatac ctctccgatc tgcaaaacca acacgatatt atcgaaatcc
     6181 tcactgttca gcgtaaagaa gaggaaacag cattaagctg tcggcttgtt ttgcgagagc
     6241 tgacagaaac agaaccggtg attattttcg aaggtatcga agcgccggca acgctgggtt
     6301 taaaagccag tcgctcggca aattatcagc gcaaaaaaca aggtttttat gcgcgctttt
     6361 ttctgactaa ctctgcacca atgttgttga ttgacccgtc acgagatgga caaatcgtcg
     6421 atgctaacct cgccgcgctc aatttctatg gttataacca tgaaacgatg tgccagaaac
     6481 atacctagga aataaatatg cttgggcgtc gcgtcatgcc tatcatgcat gaaatttcgc
     6541 atttacccgg cggtcataac cctttgaatt ttgttcataa actggcggat ggatctactc
     6601 gtcatgtgca gacctatgcc ggaccgattg aaatttatgg cgacaagctc atgttatgta
     6661 ttgtgcatga tattactgag caaaaacggc tggaggagca gctggaacat gctgctcacc
     6721 atgacgcgat gaccggatta ctgaatcggc gacagtttta tcacattacg gaaccaggcc
     6781 aaatgcagca tctcgccatc gctcaggatt acagcttgtt gctcatcgac accgatcgtt
     6841 ttaaacacat taacgatctc tatgggcatt ctaaaggtga tgaggtgtta tgcgccctcg
     6901 cccgccccct cgaaagttgc gctcgcaaag gcgatttggt gtttcgttgg ggaggcgaag
     6961 agtttgtctt attgctacca agaaccccac tggataccgc gctttcgctg gctgaaacta
     7021 tccgcgtaag cgtggcaaaa gtgagtattt cgggcttacc acgctttacc gtcagcattg
     7081 gtgtggcgca tcacgaagga aatgaaagca tcgatgaact gtttaaacgc gttgatgatg
     7141 ctttgtatcg ggcgaaaaat gatggacgca accgcgtgct ggcggcataa gccgcggatg
     7201 cgtctcgaga tcaacgactg cgcttagcgt ggcgctccca gttttcttgc ttcgcctgcg
     7261 ccgttttacg tagtgcgacg taacacgccc cgctgccgcc atgatgcggt agcgcggtgc
     7321 aatatgcctg aacatcatca aattcggtca gccagcgcgc cacatagctg cggacaatat
     7381 tggcatgtga tttatcttcc cgccctttac catgaataat cagcacatta cgcaaaccat
     7441 ccgccagggc ttgttgaatg aaactgaaca ccattttgcg gcactcttcc accggctggc
     7501 gcaaaagatt caggctcgcc tgttgcggat atttaccact gcgcagctta tccagcaccc
     7561 catgctacaa cccttcctgc cgaaactcca gcggctgact tagcgggatg atgtcgagaa
     7621 atccggtagt gaggaaatta tcaagctgca gcgtgtcgat acgctgcggc gcacgttggt
     7681 tacgcgttgg atgccagtgg acatctgtag cacgtttcag cggctggaca tcttccatgg
     7741 cgtcaagaaa cagcgatttg tcgtcaaggt tcatgtaacc tcctatgcac cttcacacgt
     7801 cgtgattcag acgggttaat tgttcatact tactgcatcg ttcccttcac agattgaaac
     7861 aatactctac ttcatacctg tgagtatctg cgcaggcggg gcattttata ctagcctgtt
     7921 agcagtatcg aacagtttag cccgagttta attcagcaca ggctccgatc aggatgattt
     7981 attcatagca ggaaccaaca aaaatgcgat aaataacaga ccactgatta gcagaaaggt
     8041 accgaaaatg ccatagtttg cgcctccctc agcatcaatt gactcaataa tttggttgaa
     8101 gccagtattt ccgatgtctg gtcacttcta ctgcgattaa aaaataaagc acttctggaa
     8161 atgaagtcat tcaccgtatg gggaacagaa accatttttt cacccattct cgaaaaatga
     8221 aaattagttc gattattgag aatagagcca cacagggcaa taccaatcgc gcccccaaga
     8281 ttacgtgtta aattaaatac accagaggct aacttcagcc gttcttttgg aattcccccc
     8341 agcgttaatg ttacgatggg ggccatcgca aactgctgcg aaatccctct aattgcctgt
     8401 ggaaacagta attcctgcca tccccattcg tgggttatag gggtaaaaag atacattgaa
     8461 aacacaaatc cgcccagccc cgccatcaac agccatcgca gatttatttt tttacttaac
     8521 cagaagtaaa agggaacaga aaataactgg aaaatacctg ttgtgcaaac ggcaaaacct
     8581 atctcttcgg catttaatcc gcgaatctgc cccaaaaaca caggtattaa atatacagta
     8641 gagaatatcc caacccctcc agaaaaagaa aaaaaacatc ccagagtgaa atttttatcc
     8701 ttaaaagcat gaagatccat tatcggattg gatattgtaa gtgtacgcgc agcaaataaa
     8761 ataaaactca ccaatgccag aactgatgtc agcaaaatag tattatcatc cagccatccc
     8821 cagcgcgctc cttcttcaag ggtatattcc agacatccca gagtcatggc caacaggata
     8881 atactcggat aatcagcaac tttcagtaaa gagagatcag gtttatcaaa attaaccaga
     8941 aaaggaatac tcaaaactaa
//
LOCUS       DAZXUK010000161         8954 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZXUK010000161 DAZXUK010000000
VERSION     DAZXUK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8954
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.161"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(11..2554)
                     /gene="mdoH"
                     /locus_tag="ACH3L8_002895"
     CDS             complement(11..2554)
                     /gene="mdoH"
                     /locus_tag="ACH3L8_002895"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFT4085930.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2547..4082)
                     /gene="mdoG"
                     /locus_tag="ACH3L8_002896"
     CDS             complement(2547..4082)
                     /gene="mdoG"
                     /locus_tag="ACH3L8_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFT4085931.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4476..5633
                     /gene="mdoC"
                     /locus_tag="ACH3L8_002897"
     CDS             4476..5633
                     /gene="mdoC"
                     /locus_tag="ACH3L8_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFT4085932.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5641..7122)
                     /gene="clsC"
                     /locus_tag="ACH3L8_002898"
     CDS             complement(5641..7122)
                     /gene="clsC"
                     /locus_tag="ACH3L8_002898"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFT4085933.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7064..7597)
                     /gene="ymdB"
                     /locus_tag="ACH3L8_002899"
     CDS             complement(7064..7597)
                     /gene="ymdB"
                     /locus_tag="ACH3L8_002899"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFT4085934.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7692..8003)
                     /gene="ymdA"
                     /locus_tag="ACH3L8_002900"
     CDS             complement(7692..8003)
                     /gene="ymdA"
                     /locus_tag="ACH3L8_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFT4085935.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8124..8456)
                     /gene="csgC"
                     /locus_tag="ACH3L8_002901"
     CDS             complement(8124..8456)
                     /gene="csgC"
                     /locus_tag="ACH3L8_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFT4085936.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8515..8880)
                     /gene="csgA"
                     /locus_tag="ACH3L8_002902"
     CDS             complement(8515..8880)
                     /gene="csgA"
                     /locus_tag="ACH3L8_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFT4085937.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT         2236 a         2319 c         2124 g         2275 t
ORIGIN      
        1 aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga ttgagcttta
       61 tcccttcgta ataactcacc catgaagaat atctttcagg ggaattccag acgcggaaat
      121 gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg cgattcagct
      181 tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc acgggcgatt tccagcacct
      241 tactggcgcg gtgacgcgct gtcgccattg cggttgccag agcgttaaat gacgggttaa
      301 acactgcgtg cataaagcca tcgtcaaggg agcgttggcg attcatctca aggaaccgat
      361 cggtatcaac cagcacctgc ggcggtgaat actcttccgg gatcaggaac agtttccagc
      421 gtttggtgcg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac ggtgacagga
      481 tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccagc cacgccatcc
      541 caacagccca cactaacccc aacagcagct gtgagccgtg gcgtttgaac gcttcgcccc
      601 atgaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag ccaaggaacg
      661 cgctgacaac gaagaccgta tggaacagca tgcgtaccgg agccagcagc acggaaaaca
      721 gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccgtattct ttcgttcctt
      781 tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc atggtcgaag
      841 caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac aactgccgtg
      901 gttgcaggaa gtattgcggt tcggtcaacg catgcactac ctgcaatgca gtagagagcg
      961 cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc aggaacaccg
     1021 cacggtgaac cgggtgcata cccttaacca ggaacagacg gaagttcatc aggttaccgt
     1081 ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggcaat tcttcataag
     1141 aacccgggag atcgtaagca atccagaccc cccaacctgc acggcgcatc aacgccgctt
     1201 ctacgaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc agcggagcca
     1261 gtgcgcagtg ctcgataaat ggtttcacgc ggataatcgc gttatgtccc cagtagtgcg
     1321 actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac acgcgggtcg
     1381 cgaactgctg acagcgcgca tacagcgtat ccataccgga cgctttcggc gacgactgaa
     1441 tgatcccggc gttcgggttg gcttccatca agcgcaccag gccgcataaa caatcaccgg
     1501 tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc cagcgacggc
     1561 agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg cgatagaaaa
     1621 tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc tctgcgacgc
     1681 agatatccgg gttataactg tcactcagaa tgtagacatc aaagtgtttg gcattcccgg
     1741 tggcttttac tgattcccac gttgcacgca agccagcaaa aacacggttc acgtcttcgt
     1801 tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca tcgccaactg
     1861 ttgacgcaga tatactgtat ttatcgcgac caataagcag ttgcaggaag cccatcaacg
     1921 ccgtccagaa tccggcggac acccaacaga acagtaccgc aaaaaggatc aggataccgg
     1981 tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga ccaaccatat
     2041 ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata taccaggtcg
     2101 cgacaacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg gtaccaacgg
     2161 tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga tagcgcggcg
     2221 tgacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta cgccacgggt
     2281 cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt aagctgatcg cgcccttcgt
     2341 cgtctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc agacgcgcct
     2401 ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt gcggtgttcg gcatccagcg
     2461 cctgatgaac ggcgcggata tcagtcttcg gcaatgctgc tttctcgctt gcggcgatgg
     2521 gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta gctccaggtt
     2581 tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtgat tttcttggca
     2641 tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt atagcgcacc
     2701 gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac cggggtatcc
     2761 tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat agtaccgtca
     2821 ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt ttgttgcacc
     2881 catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat ggtgtattta
     2941 aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc gacgatgtta
     3001 tcgttggttt catcgttggt tggaatttcc accagctcaa cgctgccttt gccccactcc
     3061 cctttcggag tcacccatgc gcttggacgg agatcgtaac gatcatcgag atcttcaaag
     3121 cgggagaaat cacgacctcg ttgcaacaga ccaaagcctc gcgggttttc catggagaag
     3181 ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc gccattacca
     3241 gcatggatag acagaccgtt agagtcgtgc aactccggac gatagttatt tgcaggcgac
     3301 ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag tttgccgact
     3361 ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc tggcataacc
     3421 acgaatttgt aagcacctgt cgcacgcggc gagtcaagca atgcataaat ggttaaacgt
     3481 ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa ttcttcaccc
     3541 gatggcaaag cggtatcaat tgctaggccg cgggcagaaa ggccataaac ctgacctgca
     3601 ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt tttatctttg
     3661 ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac cgtgtctttg
     3721 tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg tttgactgcg
     3781 gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg atggtagaac
     3841 tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt aaactggatc
     3901 tgctgatagt ccgcgtattt catgtcgcgg aaaacggagg gcaagttgct tttaggcgcc
     3961 tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat actgaaagcc
     4021 cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg cattttcatc
     4081 atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct tatatcgatc
     4141 cgatctattt taatggataa ccagccattc cgacaactga atcccggtat cgttcagcac
     4201 gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc ttctcctaaa
     4261 gacagaagca tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa ccgtcttaca
     4321 gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaacct tgaagcctgc
     4381 gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata acccgacagg
     4441 gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc gcgcaacgtg
     4501 aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct tttcatattt
     4561 ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca ttgtggctaa
     4621 ccctttttaa tgacttcatc cactcgttcc gcatgcaggt atttttcgtt atatccggat
     4681 acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa gtacgtgtcg
     4741 aacgtgtagg tatcccgatg ttaacagcca tccccctact gacattaccg caatttatta
     4801 tgctgcaata tgtcaaaggg aaagcggaaa gttggcctgg gctatcattg tatgacaaat
     4861 ataatacgtt ggcctgggaa ttaatatcac acctgtggtt tttactggtg ttagtagtca
     4921 tgacgacgct gtgtgtatgg atatttaagc gcatcagaaa taatttagaa aattctgata
     4981 aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttatgc ctcggcatcg
     5041 gctatgcggt aataagaaga acgattttta ttgtgtatcc acccattctg agtaatggca
     5101 tgttcaattt tattgtcatg caaacgctat tttatttacc gttctttatc ctcggcgcac
     5161 tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt ggctgtaccc
     5221 tcgctgcagc attggcgttt gtcgcttatt tactcaacca gcgctatggc agtggcgatg
     5281 cctggatgta cgaaaccgag tcggtgatca ccatggtcct cggcctgtgg atggtgaatg
     5341 tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg acttattttg
     5401 ttaacgcatc gctgtttatc tatctggttc accacccgtt aacgctgttt ttcggcgcat
     5461 acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg atattcgtag
     5521 tagggattgc gataattctg tatgaaattc atttacgcat cccgttactg aagtttttgt
     5581 tctctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt taagccacat
     5641 ttacaataac cattccacgg gcaatatcga cgccagtctg accataaccc gcttccagaa
     5701 actggtggcg ggttcttttt tgagaataac ctctttcttt gcatgacgat caacccagtt
     5761 gatccgtccc cacctgtcca gacggagctg ccaggccgca tcgtactggc tctgaataaa
     5821 gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca tttcagtatt
     5881 gagcaatgtt gaacgcggat cgaaattgaa agagccgata aacaccgttt taccatcgat
     5941 gctaaaagtt ttagcatgca ggctggcacc ggaattaccg gttatgccgc gatcgtgtaa
     6001 cgtactgctt tgttcacgcg tcggcttgag ttcatataat tccacgccat agcggagcaa
     6061 ttttttgcgc cagcgcgcgt atccggcatg gacgacagca acatcgttag cggcaagaga
     6121 attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt gcgccacacc
     6181 tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg gtgagcccat
     6241 gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct cccctttcgc
     6301 cggatcatca cttaataaac gtgtcttcgc ccagataagc ggcaacgttc catcaaccag
     6361 atgatttata aatggactgg attccatttt gcgtaaataa cgatgcgtca tggcatcgtt
     6421 atgccaggag gcgggtaact cgatgcgatc cgccatttca ccttccggga catccagcac
     6481 ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat cggcaacatc
     6541 ctctaccacg gggcctattg ccatgacatc taaatccgaa aaaagtggct cttcccctgc
     6601 accaaaatag gcatcaccaa tatttcgtcc tcccactagg gtaaccacgc catcgacagt
     6661 gaaactttta ttgtgcatcc ggcgattgag acgggaaaag tcggtgatat atccaagcgg
     6721 acgtagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcgtg gatgactgtc
     6781 aagcaagcgc aaaatgtcgt caagtccggg cgtattgttg tcgtccagca acaaacggac
     6841 gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgcc ccgacatatc
     6901 gtcctgccag atgtaatact gaacatccag cgtatgctct gccatttcgg ccagacgata
     6961 ccgggcggca aacgcatcca gacttttctc cagagggaaa agaccacact gaccgggatg
     7021 ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca tctccttgtt
     7081 gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca aagtatacct
     7141 gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt tttaactgcg atttcagccg
     7201 ctgccgcacg agggtaacta taaaccccag tactgattgc aggaaaagcc actgacgtat
     7261 agctgtttgc cgccaccagt cgtaggctat tcagataggc atcctgcaaa agctggtctt
     7321 cgttttgttc cccaccacgc cagactggcc cgacggtgtg tactacggct ttagcgggaa
     7381 gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc tgttgcctga
     7441 cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc ccatcgacgc
     7501 cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg gccagtttgg
     7561 taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac accttcttgt
     7621 ttcttaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag caacgtgagt
     7681 tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg tttatcaaga
     7741 tactgcactt ttactgaggc tatttgcgcg tttttgacat tgccgctcat taacgctttg
     7801 ctggagtagg ttttgcccgg aatagaacca ttctgtgggc agttcatttc tacgggtgat
     7861 gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaaatg gatcacgccc
     7921 ccttgcgcgg tgcttcctgc aatggcaata aaaggaaaaa acaaactgcc gagcataaga
     7981 gagtctaaaa aaggtctgaa catttattct atttcctcaa tgattagtca tccttgaagg
     8041 ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct ccagattcat
     8101 cttatgctcg atatttcaac aaattaagac ttttctgaag agggcgtcca ttgttgtgat
     8161 aaatgaagtg cctgtccatc agaaacagta acaactattt tcacccgatc ttccggggaa
     8221 atatttaaac tcaacttcgt caaagcaatg ggttgattag caggcaatga gagggtcttt
     8281 tcttgcttcg tctgactttg ccctgaactg ccttcgcgca gggacaatat ttgtactctg
     8341 cacagacaag attgagtaag agtaacttca ggaataatgg tgtacatatc cccttgctgg
     8401 gtcgtattaa aggttatctg gctggaaagt gccgcaagga gtaataacgc attcatattc
     8461 ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta atacaaatga tgtattaata
     8521 ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc acgttgacgg aggagttcga
     8581 tgcagtctgg tcaactgcag caccgttgcc accaccgaac tgtttaacag tcatttcaga
     8641 atttttgccg ttccactgat caagagtggc gctgttaccg aagccacgtt gggtcagatc
     8701 gattgagctg tcatctgagc cctgaccaac atctgcacca ttaccaccgc catgctgggt
     8761 aatagtcaag tcagagttac gggcatcagt ttgcagagca agtgcagagt taccgccacc
     8821 gtactggtaa atgttcagct ctgaatttgg gccgctatta ttactgaggt gtactggcaa
     8881 tagcggacac taccatttgt tcttttttaa gcagccatct gatgatattt ttccctgaag
     8941 gctgccgggg agat
//
LOCUS       DAZXUK010000162         8871 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZXUK010000162 DAZXUK010000000
VERSION     DAZXUK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.162"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..438
                     /locus_tag="ACH3L8_002903"
     CDS             <1..438
                     /locus_tag="ACH3L8_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFT4085938.1"
                     /translation="QKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQ
                     FAVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYA
                     KAKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="ACH3L8_002904"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="ACH3L8_002904"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFT4085939.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="ACH3L8_002905"
     CDS             complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="ACH3L8_002905"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFT4085940.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="ACH3L8_002906"
     CDS             complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="ACH3L8_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFT4085941.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4440..5147)
                     /locus_tag="ACH3L8_002907"
     CDS             complement(4440..5147)
                     /locus_tag="ACH3L8_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFT4085942.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="ACH3L8_002908"
     CDS             complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="ACH3L8_002908"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFT4085943.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6005..6208)
                     /locus_tag="ACH3L8_002909"
     CDS             complement(6005..6208)
                     /locus_tag="ACH3L8_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFT4085944.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6367..7875
                     /locus_tag="ACH3L8_002910"
                     /pseudo
     CDS             6367..7875
                     /locus_tag="ACH3L8_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7872..8768
                     /locus_tag="ACH3L8_002911"
                     /pseudo
     CDS             7872..8768
                     /locus_tag="ACH3L8_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            complement(8784..>8871)
                     /locus_tag="ACH3L8_002912"
     CDS             complement(8784..>8871)
                     /locus_tag="ACH3L8_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFT4085945.1"
                     /translation="VRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         2061 a         2415 c         2273 g         2122 t
ORIGIN      
        1 cagaaggtgg cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc
       61 gtgttttatg tcgcgggaga taaagtcggc atcgttcgtc tggatgcttt taaagccagt
      121 aaagatattc agtttgcggt gcagtcagga ccaatgttga tggaaaacgg tgtaattaat
      181 ccgcgtattc atcccaacgt cgcctcacgt aaaattcgta acggtgttgg gattaataaa
      241 catgggaatg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
      301 tatgccaaag cgaaactgaa cgttgagcag ctgctttatc ttggtggcac gatttcacat
      361 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
      421 gtcgagcgga aaggttagaa tgatagcttt gtgtcagata ctaaatatgc tgctgatgcc
      481 ggatgcggcg taaacgcctt atccggccta catggcgttg caatttattg aatctgaatg
      541 attttttagg ccaaataagg tgcgcagcac cgcatccggc aacgttacac cgactccagc
      601 cgggcgtaag ctgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg
      661 ctatgttcac cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgt
      721 acacgctggc cgagcttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga
      781 tggctgaccg ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc
      841 aactcgccaa taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt
      901 tccgcgtagg tcagcgtcag tttctgcatc gcgcgggtta cgccaacgta ggccagacgg
      961 cgttcttctt ccagacgccc gccttcatcc agcgacatct ggcttgggaa catgccctct
     1021 tccataccga cgataaacac ctgcgggaac tccagacctt tcgccgagtg catggtcatc
     1081 agttgtaccg catcctgcca ggtatccgcc tgcccttcgc ctgcttccag cgccgcatgg
     1141 gataggaacg cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc
     1201 gttgccgtca ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc
     1261 tgctcgtaca tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc
     1321 ggcatatcgg cagtttcctg cgctaaggcg tcgatcagtt ccataaaccg ctgtaaggcg
     1381 ctggcagcac gtccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt
     1441 gttaactggc gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc
     1501 cgcgttggcg tattcaccac acgctcaaag gccgcgtcgt cgttacggtt ggcaatcagg
     1561 cgcagatacg agagcgcatc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa
     1621 atacggtacg gcatactcgc ctgcaacagt gcttcttcca gcacacgcga ctgggcgtta
     1681 ctgcggtaga gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg
     1741 cggttaacca caaaacgcgc ttcatcgagt tcgttaaaag cgcaatagag ggaaataggc
     1801 tcaccgtccg cgccatcggt ccacagtttt ttacccagac gcccgttatt gttttcaatc
     1861 agggcgttag cggcgctcag aatattgctg gtagagcggt agttttgctc cagacgaata
     1921 atttcggcac cggggaaatc attaaggaaa cgctgaatat tctccacctg cgccccgcgc
     1981 cagccgtaga ttgactggtc gtcatcaccg acgatcatca ctttgccggt gtcgcccgcc
     2041 agcaggcgga tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggata
     2101 ttggtaaaac gttcgcggta gtgttgtagg atatgcggct tgttaagcca cagttcgtga
     2161 gcgcgcagca gcagctcggc gaagtccacc aggcccgcgc gatcgcacgc ttcctgatac
     2221 gcctgataca ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc
     2281 ggacgcaggc cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac
     2341 tgcttctcgt cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttcg
     2401 ctatcgagga tctggaaatc ctgcggcaga ttggcgtcca tatggtgcgc acgcagcagg
     2461 cggtgcgcca gcccgtggaa ggtgcctacc cacatgccgc cctggctggt gcccatcagt
     2521 tgtccgatac gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc
     2581 gagtatggcg agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc
     2641 gtcttaccac tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct
     2701 tcgcgttgtt tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg
     2761 taaaaatagg ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca
     2821 gatttgctga ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgtggca
     2881 gtaaacggct gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct
     2941 gcattccgct gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga
     3001 tctcgccgat cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt
     3061 ttgagcgccc gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca
     3121 gttccggctg ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt
     3181 ttaaggtgtc gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa
     3241 tcattgctgc gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg
     3301 cttgttcaat cgaacgaaaa cgccaacgtg tcacgtcgtg ataaatctcg ggttccgctt
     3361 cccgtaccgc ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat
     3421 gataattttg cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat
     3481 cgtaaagggt atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa
     3541 aacgcattat ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg
     3601 ttgaaaatca agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagtagctc
     3661 ctgcacacca cgaagatcgc cgctcgactc cagcatatgc gtagcgaacg agtgacgtaa
     3721 tttatgtggg tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt
     3781 ctgcacatta cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc
     3841 gctaccaaac aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc
     3901 aatcggcagg cggcgctctt tgctgccttt ccccatcacc cacacttcgc cggactccag
     3961 gtcgaggtgt ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat
     4021 cacttccagc attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tatccagcag
     4081 ccgattcata tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga
     4141 aactcctttc gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct
     4201 acgtagcgca gaaagccgta acgccagact tgctgctccc agccctttac ggcgactgcg
     4261 tacagcaaaa ttgcgtacca tcgctgcatc gcattgttgc cagctttgca ggccgttttc
     4321 gctggcaaaa ttgatgatcg cctcaagctg acgctggtag ttaagtaggg ttatcgggct
     4381 aagctgacgc tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt
     4441 catacgcgtt caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt
     4501 aactgcgttc cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacaaca
     4561 cccaaatcag catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc
     4621 ggtagcacca ccagcagctc cggtccgtta agcggcccaa gatagtgctg ttcctgcccc
     4681 aaacgctgaa tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc
     4741 gacggcgcac ctaagcgcca gcgatctgga aacaggcgca gactcgcacc agctaggccg
     4801 agatcgcgcg cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg
     4861 agactgcgct gcaggtagag taggcgataa aacagccctt cgttggcgat agcctgctcc
     4921 atcaacaacg ccatgttctc ttccagaaca tgaatgtgat tacgtgcgcg ggccatgtgc
     4981 cactcgacca acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg
     5041 cgcgcattgc ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca
     5101 tcaagctccg tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc
     5161 catcgtagac atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg
     5221 cgatatcaag acgaccgccg gggagttcca cgcgtacttc ttcgtccagc aaaccttgct
     5281 gaatccctac cgccaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc
     5341 cacgctcata aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat
     5401 tggcgcgctc cggaaaacgc tcgtgacttt ccagaacagg accaagcgtt tctaccgccg
     5461 cggtatcgac atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc
     5521 ataagattgt ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa
     5581 acggcacggc ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat
     5641 cggtgacggt cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg
     5701 tcagtccttt cagacgcaca aaacgggcaa agcagcgcgc accgttaccg cactgcgcca
     5761 cttcactgcc atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg
     5821 gctcaaccac cagcagctgg tcaaacccta cccccaggtg ccgatccgcc aggcgacgaa
     5881 tcagctccgg tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc
     5941 caaggccatg cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg
     6001 acttttagta attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca
     6061 ccgtggattg cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag
     6121 gcgggaaata gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata
     6181 cagtgagtgc cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg
     6241 ctttctatca tcgcaggaga aggcgtaaaa gcaagcgttt agcgactctt tatgaagtct
     6301 gaaaagtgag gaaatgctgc gagtataacg cttactcgtg aggatttatg ttctgctggc
     6361 agtttcgtga tcaacacctc gcgccaggat ggctttatgc actatcgcaa tgtggtttgt
     6421 ttgttgtctt gttcattgtt tttatcgtcg gcatggggat gtcggctttc cgcacctgaa
     6481 cataatattt accagaaaca gggaaaaggc gtggtgtacc tgcgccctta taaagaaaca
     6541 aatctttctc tacctgacgt gaattacaag agtttgcgtc ggttacccaa tttattaata
     6601 gacccgacaa cactagacga atgggataaa gaaccgcctc tgactgattt gacaaccgac
     6661 tacctctatg agggcgcaca ggcctggtat cctcattatt catggcatag cgatggtcgc
     6721 aatatcctct acgcgggtga agttgtgcaa aaccctcccg gcacacctcc cgtcgatgtc
     6781 gcctcgttca aggcatgggg tgattttgcc gcagataaac acagtctcta ttttgaaggc
     6841 aagcgcaccg atgacaatgg tggtggaaat agcttggata tcaaaacgct gcatcaggtg
     6901 gaattccggc ctccctggga tcctgactta ctggggctga tattacgtga cgctaatttc
     6961 ttgtatatca atgggcatcg cctcgctgac cccgagagtt tccgtgtgct ggcgcagaag
     7021 tcctgggatc agcgaggaaa gttttccacc acgtttaacc cctgcattgc cgtaccattt
     7081 ggcccctggg ataccctggc tcgtacacgg accaaaatcc tgctcaacgg cgaacagctt
     7141 gatgccgacc cggacacctt ttccgtcgtg cgctggatgc ccggctcact cttgacctgg
     7201 cgtgataaaa acgggctgca gcgtaaagtc ctcgacaagg aaaatctggc gtgggatgaa
     7261 gatttaacaa agcactgtct ggatttttct ctgctggaaa agaaagtgtt ctggcgtaaa
     7321 ggacctgctt gtaaacagga agaattaccc ggactcgatc cggagcagtt tcaacccatc
     7381 agtgatgctg tcgcccagca tcaggactcg ctttatacca tcatcgaaac agagtctggt
     7441 aaccgtaagc tggagatcgt gaaacttgat gatcccaatc ttattatcaa caaacgtttc
     7501 aacgccggga aacgccacgg ctatttactt acgcgtgccg aagggtagcc ataccattcc
     7561 ggtttacacg tgtttgaatc tgacggaccg ctgatcttac tggataaccg ttctccggat
     7621 gaacgcgaag cccatcttaa tgaccatccc tttttgcgca gatggtatgc ccgcgataac
     7681 cgctacattt acagctttga tggcgcgcag ctctggcgat accgcaccgc tgatccgaaa
     7741 caagttcgct taatctggaa ggaacaacat tcgggatatg tctatggcgt aaattacaaa
     7801 acgggatatc tggacggaaa aattactgat gacggcgaat ttattcctgc cccgcgcaat
     7861 gaggcgacaa aatgaagggt caaataatca cagttgcact catcctgttg ggacacacat
     7921 ttctgacgcc tgctgtacag gccatagggt tcgactatta caatgatcac ggtgttatgt
     7981 cttacggtaa aggttattgg ggggataaga aaatcattgc ccagtttccg gagatgagca
     8041 gggccggttt gcgccttgtg aaaaatatct ctgggaaaac gggttatata ccttccgatg
     8101 aaataagcat agaaaatgag gaataccact gggtgacaga cggccgcgtt attctctggc
     8161 gtggcaaaat cgttagcaat ccaccgggaa cactcactgt cgatattgcc agctttcagg
     8221 ctatgggccg cttcgcggtc gataaatata gcctctattt tgacggacag cgcaccgaaa
     8281 gtaatagcgg cgcgtcacgt gttgagctgg cgacactaaa agctatcgaa ggtaactcta
     8341 ccacgctggt ggatagtaaa aatctctact tgtccggtcg acgtcagggg agcagcagtg
     8401 atgttactgt attagaaaaa agatggtggg gtattaatcc acgtcttatg agtgtaaaca
     8461 gaaattcata ttccaatgat ctgcttatcc gcagtgggca gaatatttat ttaaatggcg
     8521 ttcaccttac ggcgaatgca gactcatttg agataattcg ctggatacct cattcactgc
     8581 tggtttttcg cgacaataag ggtctgcatc gttatccctt tggtcaatta tcaggcaaag
     8641 cgatacccgt agatgatgac gtctcttttg aagtagggga aagtcgcgtt cgctggcgta
     8701 aacagctcac gcccgaccgt cagtggagca agtggataga cctaccaggt attgaacctg
     8761 aacaattcgg taatgactcc aacttactga tagtgtttta tgttcagata atgcccgatg
     8821 accttgtcat gcagctccac cgattttgag aacgacagtg acttccgtac c
//
LOCUS       DAZXUK010000163         8836 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZXUK010000163 DAZXUK010000000
VERSION     DAZXUK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.163"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(62..406)
                     /locus_tag="ACH3L8_002913"
     CDS             complement(62..406)
                     /locus_tag="ACH3L8_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFT4085946.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            538..2448
                     /locus_tag="ACH3L8_002914"
     CDS             538..2448
                     /locus_tag="ACH3L8_002914"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFT4085947.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQIVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2506..3201
                     /gene="tsaB"
                     /locus_tag="ACH3L8_002915"
     CDS             2506..3201
                     /gene="tsaB"
                     /locus_tag="ACH3L8_002915"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFT4085948.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3241..3822
                     /gene="yeaY"
                     /locus_tag="ACH3L8_002916"
     CDS             3241..3822
                     /gene="yeaY"
                     /locus_tag="ACH3L8_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFT4085949.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4028..5713
                     /gene="fadD"
                     /locus_tag="ACH3L8_002917"
     CDS             4028..5713
                     /gene="fadD"
                     /locus_tag="ACH3L8_002917"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFT4085950.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            5795..6910
                     /gene="rnd"
                     /locus_tag="ACH3L8_002918"
     CDS             5795..6910
                     /gene="rnd"
                     /locus_tag="ACH3L8_002918"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFT4085951.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(6964..7929)
                     /gene="yeaX"
                     /locus_tag="ACH3L8_002919"
     CDS             complement(6964..7929)
                     /gene="yeaX"
                     /locus_tag="ACH3L8_002919"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFT4085952.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(7985..8602)
                     /gene="yeaW"
                     /locus_tag="ACH3L8_002920"
                     /pseudo
     CDS             complement(7985..8602)
                     /gene="yeaW"
                     /locus_tag="ACH3L8_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
BASE COUNT         2115 a         2212 c         2384 g         2125 t
ORIGIN      
        1 atgtaaaacc gaccagtatt atcgataaat tatcgtctga cttattttgc ttcgataaag
       61 cttacaccgc agccacaatc ttaatttcaa ctttatactt cgggttcatc aaacccgctt
      121 gtaccgtgca gcgcaccggc gcatgacccg cgacaaccca ggcatcccac gctttattca
      181 tcgccgcgaa gtcgttttta tcggccagga aaatggtggc atccagaatg ctcgatttat
      241 tgctgccctg tttttccagc acggcgtcaa tctgtgccag cgtgttggcg gtttgctcaa
      301 aggcatcggc gtcgaggttt tccggtacac cagtgtagta gagcgtgttg ttgtggatta
      361 ctacatcaga ccagcgggct tcagcatcga tacgaacgat agtcatcatt ttgtcctcat
      421 tatacttcca ttttcgtgct gcaaagactg ccacaatgtg aggttgccgt caccacttca
      481 actggcgaaa gcgccctcat cctgacataa tccctgttca aatcaacagg gggtagtgtg
      541 acggacgatt ttgcaccaga cggtcagctg gcgaaagcga taccaggctt taagccgcga
      601 gaaccacagc gacagatggc ggtagccgtc acccaggcga tagaaaaagg ccagccgctg
      661 gtggtggaag caggaaccgg tacgggcaaa acctacgctt acctggctcc tgcgctgcgg
      721 gcgaaaaaga aagtcattat ctcgaccggc tcaaaagcgt tgcaggatca gctctacagc
      781 cgcgatttgc caacagtctc aaaggcattg aaatatacgg gcaacgtggc gctgctgaaa
      841 gggcgctcaa actacctctg cctcgaacgt ctcgaacagc aggcgctggc ggggggcgat
      901 ctgccggtac aaatcttaag cgatgtgatc ctgctgcgct cctggtctaa tcaaatagtc
      961 gatggtgata tcagcacctg cgtcagcgtg gcggaagatt cacaggcgtg gccgctggtc
     1021 accagcacca acgacaactg tcttggcagc gactgcccga tgtataaaga ttgctttgtg
     1081 gtcaaagcac gtaaaaaagc gatggacgcc gatgtggtgg tggtaaacca tcatctcttt
     1141 ctggcggata tggtggttaa agagagtgga tttggcgagt tgatccctga agcggacgtc
     1201 atgatcttcg acgaagccca ccagctaccg gacattgcca gccagtattt tggtcagtca
     1261 ctctccagtc gacaactgct cgacctggca aaagacatca ccatcgccta ccgcaccgaa
     1321 ttaaaagaca cccagcagtt acaaaagtgc gctgatcgtc ttgcccagag tgcgcaggat
     1381 tttcgtctgc aactcggtga gccaggttat cgcggtaacc tgcgtgagct gttagctaat
     1441 ccgcaaattc agcgggcatt tttactgctc gatgacacct tggaactttg ttatgatgtg
     1501 gcgaaactgt cactggggcg ttccgccttg ctggatgcgg catttgagcg cgccacgttg
     1561 tatcgcacac ggctgaagcg gctaaaagag atcaatcagc cgggctacag ctactggtac
     1621 gaatgcactt cgcgccattt tactctggct ctcacgccgc tcagcgtggc ggataaattc
     1681 aaagagttaa tggcgcaaaa gcccggtagc tggatcttca cctcagcaac gctgtcggtg
     1741 aacgacgatc tgcatcattt cacctcgcgg cttgggatag aacaggcgga gtcgttgcta
     1801 ttgccaagcc cgtttgatta cagccgccag gcgttactct gtgtgccgcg caatctgccg
     1861 caaaccaacc agccaggttc tgctcgccag ttagcggcaa tgctgcgacc gatcatcgaa
     1921 gctaacaacg gtcgttgttt tatgctttgt acctcgcacg ccatgatgcg cgatctggcc
     1981 gagcagttcc gcgctaccat gacgcttccc gttttgttgc agggggaaac cagtaaaggc
     2041 caactgttgc agcagtttgt cagtgccggt aatgcgcttc ttgtggcaac cagtagtttc
     2101 tgggaagggg tggacgtgcg tggcgataca ttgtcattgg taattatcga caaattgccg
     2161 tttacctcgc cggatgatcc actgttaaaa gcgcgcatgg aagattgtcg tttgcgcggt
     2221 ggcgacccgt tcgatgaagt gcaactacca gatgccgtca ttactctcaa acagggggta
     2281 ggtcgactga ttcgcgacgc cgacgatcgt ggcgtgctgg tgatttgtga ctatcggctg
     2341 gtgatgcgtc cttacggcgc gacgtttctc gccagtctgc cgcccgcgcc acgcacccgt
     2401 gacattgccc gtgcggttcg tttccttgcg ataccatcct ccaggtaatt tgttacggag
     2461 tatggtatga tgcgcgcctt tttattgatc taagcacgaa gacccatgcg aattctggct
     2521 atcgataccg cgacagaggc ctgctctgtc gccctgtgga acgacggtac tgtcaacgct
     2581 cattttgagc tttgccctcg tgaacatact caacgaatct taccgatggt gcaggatatc
     2641 ctgaccacca gcggaacttc cctgactgat attaacgctc tggcttacgg gcgcggcccc
     2701 ggtagcttta ccggcgtgcg cattggtatt ggcatcgcgc aagggctggc gcttggcgcg
     2761 gaattaccga tgattggcgt ctccacgcta atgacgatgg cgcaaggggc gtggcgcaaa
     2821 aacggcgcaa cacgcgtgct gtcagccatt gatgcgcgaa tgggcgaagt ttactgggcc
     2881 gaatatcagc gtgatgaaaa cggtatctgg cacggtgaag aaaccgaagc cgtactcaaa
     2941 cccgaactcg ttcatgaacg aatgcaacag ctttccggcg aatgggtgac ggtagggacg
     3001 ggctggcaag cctggccgga tctcggtaaa gagagcgggc tggttttgcg cgatggcgaa
     3061 gtgttactcc ccgctgcaga agatatgctg ccgattgctt gtcagatgtt tgccgagggt
     3121 aaaacggtgg cggtggaaca tgccgagccg gtttatttgc gtaacaacgt cgcatggaag
     3181 aaacttccgg gcaaagaatg aatcttaaga gtatgccctg agaaaaaaag gagtcgcatc
     3241 atggcggttc aaaagaatgt tatcaaaggc atactggcag gtacgtttgc gctaatgctg
     3301 agcggttgtg tcactgtgcc ggacgccatt aaaggcagca gtaccacgcc gcaacaagat
     3361 ttagttcggg tgatgagtgc gccgcagctg tacgttggtc aggaggcacg ctttggtggc
     3421 aaagtggttg cggtacaaaa ccagcaaggg aaaacccgcc tggaaattgc taccgtaccg
     3481 ctggacagcg gagctaggcc gacgctggga gaaccttctc gcggtcgcat ttatgccgat
     3541 gtgaacggtt ttctggaccc ggtggatttc cgtggacaac tggttacggt agtcgggcca
     3601 atcaccggtg cggttgacgg caaaatcggc aatacgccct ataaatttat ggtgatgcaa
     3661 gtaacgggtt acaaacgttg gcatttaacc cagcaggtga ttatgccgcc tcagccgatt
     3721 gatccatggt tttatggcgg tcgtggctgg ccctatggct acggcggatg gggctggtat
     3781 aatcccggcc ccgcgagagt acaaacagtt gtaaccgaat aattgcttgt ttttaaagaa
     3841 tgcagaaaca gcggctggtc cgctgtttct gcattcttac ggtaaagata aaaataaata
     3901 gtgacgcgct tcgcaacctt ttcgttgggt aattatcaag ctggtatgat gagttaatat
     3961 tatgttaacg gcgtgtatat catttggggt tgcgatgacg acgaacacgc attttagagg
     4021 tgaagaattg aagaaggttt ggcttaaccg ttatcccgcg gacgttccga cggagatcaa
     4081 ccctgaccgt tatcaatctc tggtagatat gtttgagcag tcggtcgcgc gctacgccga
     4141 tcagcctgcg tttgtgaata tgggggaggt gatgaccttc cgcaagctgg aagaacgcag
     4201 tcgcgcgttt gccgcttatt tgcaacaagg gttggggctg aagaaaggcg atcgcgttgc
     4261 gttgatgatg cctaacttat tgcaatatcc ggtggcgcta tttggcattt tgcgtgccgg
     4321 gataatcgtc gtaaacgtta acccgttgta taccccgcgt gagcttgagc atcagcttaa
     4381 cgatagcggc gcatcggcga ttgttatcgt gtctaacttt gcccacactc tggaaaaagt
     4441 ggttgataaa accgccgttc agcacgtaat tctgacccgt atgggcgatc agctatctac
     4501 ggcaaaaggc acggtagtca atttcgttgt taaatacatc aagcgcttgg tgccgaaata
     4561 ccatctgcca gatgccattt catttcgtag cgcactgcat aacggctacc ggatgcagta
     4621 cgtcaaaccc gaactggtgc cggaagattt agccttcctg caatacaccg gcggcaccac
     4681 aggtgtggcg aaaggcgcga tgctgactca ccgcaatatg ctggcgaacc tggaacaggt
     4741 caacgccacc tatgggccgt tgctgcatcc aggtaaagaa ctggtagtaa cggcactgcc
     4801 gctgtatcac atttttgccc tgaccattaa ctgcctgctg tttatcgaac tgggtgggca
     4861 gaacctgctt atcactaacc cgcgtgatat tccagggttg gtaaaagagt tagcgaaata
     4921 tccgtttacc gctatcactg gcgttaacac cttgttcaat gcgttgctga acaataaaga
     4981 gttccagcag ctggatttct ccagtctgca tctttccgca ggcggtggga tgcctgttca
     5041 gcaagtggtg gcagaacgct gggtgaaact gaccggacag tatctgctgg aaggttatgg
     5101 cctgaccgag tgcgcgccgc tggtgagcgt taacccgtat gatattgatt atcatagcgg
     5161 tagcatcggt ttgccggtgc catcgacgga agccaaactg gtggatgacg acgacaacga
     5221 agtaccaccg ggtcaaccgg gtgagctttg tgtcaaagga ccgcaggtga tgctgggtta
     5281 ctggcagcgt ccggatgcta cagatgagat catcaaaaat ggctggttac acaccggcga
     5341 catcgcggtg atggatgaag aaggattcct gcgcattgtc gatcgtaaaa aagacatgat
     5401 tttggtttcc ggttttaacg tctatcccaa cgagattgaa gatgtcgtca tgcagcatcc
     5461 tggcgtacag gaagtcgcgg ctgttggcgt accttccggc tccagtggtg aagcggtgaa
     5521 aatcttcgta gtgaaaaaag atccatcgct taccgaagag tcactggtga ctttttgccg
     5581 ccgtcagctc acgggataca aagtaccgaa gctggtggag tttcgtgatg agttaccgaa
     5641 atctaacgtc ggaaaaattt tgcgacgaga attacgtgac gaagcgcgcg gcaaagtgga
     5701 caataaagcc tgagcgttaa gtcagtcgtc agacgccggt taatccggcg ttttttttga
     5761 cgcccactaa agagaaaaca atttgaatta ccaaatgatt accacggacg atgcgctggc
     5821 ttctttgtgt gaagccgtcc gtgtctttcc ggcgatagcc ctggatactg aatttgttcg
     5881 tacgcgcact tattacccgc agctggggtt gattcaactt ttcgatggcg agcatctggc
     5941 gctaattgat cctctcggga tcaccgactg gtcaccgctg aaagcgatcc tgcgcgatcc
     6001 gtccatcaca aaatttctcc atgcaggcag tgaagatctg gaagtgttcc tcaatgtctt
     6061 tggcgaatta ccacaaccct tgattgacac gcaaatcctt gctgccttct gcggacgccc
     6121 gatgtcatgg ggtttcgctt ccatggtgga agagtattcc ggcgttacgc tggacaagag
     6181 tgaatcgcgc accgactggc tggccagacc gctgaccgaa cgtcagtgtg aatacgcagc
     6241 ggcggatgtc tggtatctgt taccgatcac cgccaagctt atggtagaaa cggaggcctc
     6301 cggctggcta cctgcggcgc tggatgaatg ccgcctgatg caaatgcgtc gtcaggaagt
     6361 cgttgcgccg gaagatgcct ggcgtgatat caccaatgcc tggcagttac gcacacgcca
     6421 actggcctgt ctgcaactgt tagccgactg gcgactgcgc aaggcgcgag agcgtgatct
     6481 ggcggtgaac tttgtcgtgc gtgaagagca tttgtggtcg gtcgcgcgtt atatgccggg
     6541 aagtttaggc gaactggaca gcctgggttt atccggtagc gaaatccgct ttcacggtaa
     6601 aacgctgctg gcgctggtgg aaaaagcgca gacattgccg gaagatgcct taccgcagcc
     6661 gatgcttaac ctgatggaca tgcctggtta tcgtaaagcg tttaaagcga ttaagtcgct
     6721 gattactgac gtgagcgaaa cgcataagat cagcgccgaa ttgctggcat cgcgtcggca
     6781 aatcaaccaa ctgctgaact ggcactggaa actgaaaccg cagaacaatt tgccggagct
     6841 gatttccggc tggcgtggtg agctgatggc ggaagcatta cacaatttat tgcaggaata
     6901 tccgcagtaa aatcttccga agccggactg ggcgcgctca gcgccacatc cggctttggc
     6961 aaactacaaa tccaacacca ggcgtttacc cttcgcacgc gaacaacaga tcaacatact
     7021 ttgctggctg gcacgctctt catcgctaaa atactgatcc cgatggtcag cttcaccttc
     7081 cagtattgcc gtttcgcagg ttccgcatac cccttcacga cataaacatt ccactttcgc
     7141 ggctttatta ttttcaataa cctgcaaaat agtcatctct tccggcacca caaactcttt
     7201 tccggaacgg gcaagcacca gggtaaatgc atcgccggtt ttgtcttcga tagcaaattg
     7261 ctcaaagtgc agtgtatcgg cggcgatgtc cagacgcgca gcttcacttc ttaccgcttc
     7321 aattagcgcc tcggggccac aggtataaac gtgtgtgcca ggttcgatat ccgccaataa
     7381 tcgcgctaat tccagtcgtg ttccggttga tgaatgatgc aaatggactt tctcagcctg
     7441 cggatgctgg actagctcag cacgaaatgc gcaactttct ggatttcgcg agcagtaatg
     7501 tagctgccag tcgatgtcgc tgtgttgcaa ctctgccatg tgcgacagga aaggggtgat
     7561 accaataccg cccgcgataa acagatgctt tctggctgag ggaattagcg caaacaggtt
     7621 attaggcgtt gagatcgtta accgatcgcc cacttttacc tgctgatgca aaaagcggga
     7681 accgccgcgc gagttttctt ccagtcgaac ggcaatctga taactagagg tgttatgcgg
     7741 tgaactcagt agtgaatacg cattgctgta ctggttatca ccatcgctca tctgcactag
     7801 gatgtgactt cctccggtga acgcaggcag cggtttgcca tcggttgcca ccagcgtgaa
     7861 gcgtttcacc tgttcggtaa ggggttcaac ctggctcacc tgtacttcaa acatttgata
     7921 gtctgacatt gctgtactcc atcaaaaatc agcccagcgg cggaaaatac cgccgccgat
     7981 gtcattagtc cttaaacacc tgcgccagca gattatggaa atgggcgata ccatgttcgg
     8041 aaatgccact accgctactg tcggccatga tgcgcccctg accacgatag ccacgcgatt
     8101 tcagcccttt ctgtacgctt tcaaccagac gtaaatcttc cggatggaac acatcgcgat
     8161 accactcaat cagcgatttt tgctcgtcgg ttaactcttc attggtgaag taaatatcgt
     8221 agttttgcag ggtagtttca gaatccaccg ggaattcata aatgaccgtc atcatccctt
     8281 tgatcggggt gacgttcagc atcgtgcacg gccacagcca gaaaccgtgg aatgccgcat
     8341 ccgtaccctc ttcaaattta aacgactgtt cggacggttt ggcgaaaccg tattgcagcg
     8401 tccagttacc gtgcatggtg tgccagtaac gatcaacctg tacggagtcg gagaaaccta
     8461 gatgcgccgg accacagtga tagcactcga gatagttatc gacaatgttc ttccagttgg
     8521 caggcgtgcg ggtggtaaag cgggccgcta gtttcagatt gtggacttcc gggcaggctt
     8581 ccagcacttt cgcgcccaga ccggtgtatt gattcacttg aagtacgaaa aaaaaccggg
     8641 aggacattgg attattcggg ttctgatggg attagatttg gtggggcttg caagcctgta
     8701 gcgcaaattt tagtcgttaa tcaatgaaac gcgaaagata gtaaaaaatt gcttttgttt
     8761 cattgaaaat acgaaaaaca aaaacactgc aaatcatttc aataacagct tcaaaaaacg
     8821 ttcaaaaccg ataaca
//
LOCUS       DAZXUK010000164         8821 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZXUK010000164 DAZXUK010000000
VERSION     DAZXUK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8821
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.164"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            143..646
                     /locus_tag="ACH3L8_002921"
     CDS             143..646
                     /locus_tag="ACH3L8_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFT4085953.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(726..977)
                     /gene="yecJ"
                     /locus_tag="ACH3L8_002922"
     CDS             complement(726..977)
                     /gene="yecJ"
                     /locus_tag="ACH3L8_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFT4085954.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1092..1178)
                     /gene="azuC"
                     /locus_tag="ACH3L8_002923"
     CDS             complement(1092..1178)
                     /gene="azuC"
                     /locus_tag="ACH3L8_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFT4085955.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1441..1764
                     /locus_tag="ACH3L8_002924"
     CDS             1441..1764
                     /locus_tag="ACH3L8_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFT4085956.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1935..2432
                     /gene="ftnA"
                     /locus_tag="ACH3L8_002925"
     CDS             1935..2432
                     /gene="ftnA"
                     /locus_tag="ACH3L8_002925"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFT4085957.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2469..2708)
                     /locus_tag="ACH3L8_002926"
     CDS             complement(2469..2708)
                     /locus_tag="ACH3L8_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFT4085958.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2898..4109
                     /gene="tyrP"
                     /locus_tag="ACH3L8_002927"
     CDS             2898..4109
                     /gene="tyrP"
                     /locus_tag="ACH3L8_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFT4085959.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4171..4836)
                     /gene="yecA"
                     /locus_tag="ACH3L8_002928"
     CDS             complement(4171..4836)
                     /gene="yecA"
                     /locus_tag="ACH3L8_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFT4085960.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5032..5118)
                     /locus_tag="ACH3L8_002929"
     tRNA            complement(5032..5118)
                     /locus_tag="ACH3L8_002929"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5082..5084),aa:Leu,seq:taa)
     gene            complement(5131..5204)
                     /locus_tag="ACH3L8_002930"
     tRNA            complement(5131..5204)
                     /locus_tag="ACH3L8_002930"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5170..5172),aa:Cys,seq:gca)
     gene            complement(5259..5334)
                     /locus_tag="ACH3L8_002931"
     tRNA            complement(5259..5334)
                     /locus_tag="ACH3L8_002931"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5299..5301),aa:Gly,seq:gcc)
     gene            complement(5486..6034)
                     /gene="pgsA"
                     /locus_tag="ACH3L8_002932"
     CDS             complement(5486..6034)
                     /gene="pgsA"
                     /locus_tag="ACH3L8_002932"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFT4085961.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6091..7923)
                     /gene="uvrC"
                     /locus_tag="ACH3L8_002933"
     CDS             complement(6091..7923)
                     /gene="uvrC"
                     /locus_tag="ACH3L8_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFT4085962.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7920..8576)
                     /gene="uvrY"
                     /locus_tag="ACH3L8_002934"
     CDS             complement(7920..8576)
                     /gene="uvrY"
                     /locus_tag="ACH3L8_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFT4085963.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8718..8789
                     /locus_tag="ACH3L8_002935"
                     /pseudo
     CDS             8718..8789
                     /locus_tag="ACH3L8_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT         2180 a         2238 c         2046 g         2357 t
ORIGIN      
        1 gttattctta tttcagatca tcgtcagaat tgactccacg atcacatttc ggaccggcag
       61 aaaggaatta ttctgcaaac agtaattatg gtgttttgat ttatcttgca cctctccact
      121 tctggatata aggatattag gtatggcaac cgctggaatg cttctcaaac tcaactctca
      181 aatgaaccgc gagttttacg catccaatct ctaccttcac ctgagtaact ggtgttctga
      241 acagagtctg aacggcaccg ccactttcct tcgcgcccag gcacagagta atgtgaccca
      301 aatgatgcgc atgtttaact ttatgaagag tgtcggcgct acccccatcg ttaaagccat
      361 tgatgttcca ggtgaaaaac tgaactctct ggaagaactg ttccaaaaaa cgatggaaga
      421 atacgagcaa cgttcaagca ctctggcgca gttagccgat gaagcgaaag aactgaatga
      481 tgattcaacc gtcaatttcc tgcgcgatct ggaaaaagaa cagcagcatg atggcctgtt
      541 gctgcaaacc attcttgatg aagtgcgcag tgcgaaactt gcgggtatgt gccctgtgca
      601 gaccgaccaa catgttctga atgtcgtgtc acaccagctg cattgatcat acatcggcgc
      661 taatacattg cgccgatgaa gtttttgaga aaccgctgcc tcatctgttt gaagcagcgg
      721 tttttttaat gggattcacc ctgtggggta aacttgagtt caatcagcgc gatggctttt
      781 tggattgccc gcatggtgac cgggtctgcg gcagcgggat gatgagtaaa gtcgatattc
      841 ttcagctgac tggacatttt ttcacgaact tcaacgggcg cgattacatc gagaacatcc
      901 agaatttgtt tgataaccaa ctggcaagct accacatcag aaaccagttc ctgatcggca
      961 ttcagcggct gggacatcgt aaactcctga tagcattttg aaagccgtta tagtagcggc
     1021 ttcacatctt cagcgatagt cacatccacc gtcatcagga cacaaaaaaa cctgccggag
     1081 caggtttttt gttatcggaa catattgcct ggcggtacgt ctttgaacgt cttgcaatag
     1141 ttattgaaca tacttttcag gattttgcgc agtttcatcg cggcactccg accatttgtt
     1201 atacaggtgt tattgtcttt gcgcttataa tatgacaacc atcacaaaaa tcaatcttta
     1261 tgtgatacaa atcacataaa taccccttta atgttataaa aatgataatc aaaaaacagc
     1321 ccccctattt ctgacaccta cagatggcaa gaaatagcgc ctgccaggcg tcttttccgg
     1381 ccattgtcgc agcgctgtaa cgcgtaaaat agtgctttct cttactcttc tggctggacc
     1441 atgagacttc tgattctgac tctttcatta ataacgctgg cggggtgtac ggttactcgc
     1501 caggcccatg tgagcgaagt tgacgcggca acaggtattg tacggttggt ttatgaccag
     1561 gcttttttgc agcatgccca taccgatcgt tatgtcagtc gcgggatcgc cgatcgcgca
     1621 tgccagcagg aaggctatac ccacgcgatc ccctttggtc agccagtagg caactgtagc
     1681 ctttttgcgg gttccttatg tttgaatacg gaattcactt tgtcatatca gtgccatcat
     1741 tccgccttcc ctgtctttct ctaaatattt taatcagcga ggggatcttc gctgattaaa
     1801 gaaatagcgg taatgcgttt tattcccatt cgtattttta ataattgaag tttatatttt
     1861 accttttgca aataataaaa taacaaatta tagtgacgcc acagcaacaa atataacctt
     1921 tgtggagcac tatcatgctg aaaccagaaa tgattgaaaa acttaatgag cagatgaacc
     1981 tggaactgta ctcttcactg ctttatcagc aaatgagcgc ctggtgcagc tatcatacct
     2041 tcgaaggtgc tgccgcgttc ctgcgccgtc acgcccagga agagatgacg catatgcagc
     2101 gtctgtttga ttacctgact gataccggca atttaccgcg tattaatacc gttgaatctc
     2161 cgtttgctga atattcctca cttgatgaat tattccagga aacctataaa cacgaacaat
     2221 taatcaccca gaaaattaac gaactggctc atgctgcaat gaccaatcag gactacccaa
     2281 catttaattt cctgcagtgg tatgtttctg agcagcatga agaagagaaa ctgttcaaat
     2341 cgattattga taaattaagc ctggcaggca aaagcggcga aggtctgtat tttatcgaca
     2401 aagaactctc taccctcgac acacaaaact aatgctctcc ggcggcaggt ttctgccgcc
     2461 gtttcaaatc agtgacggca aatcttactc tgatcggtcg aaaaaccttc ttctgaaggt
     2521 ataaatttgc cttttgctgc cagaaacgct accagctctc ccgctgtcac cccttctgcc
     2581 gagcacgtgt gaaaacgtgc ctgttcacca aaacgcgctt taatcgcagc ttccagactg
     2641 gcatgcgtat attgctcgcc tgattcaatc atcatattta acacttcatg accgtgaata
     2701 gagtccatcg ttcctcctca aaaaaagcct agcgtagcga ttaccgctta tgaagacttt
     2761 gcgccagcgc aggactgaat gcttttattg tacatttata tttacacaat atgtaacgtc
     2821 ggtttgacga agcagccgtt atgccttaac ctgcgccgca gatatcactc ataaagatcg
     2881 tcaggacaga agaaagcgtg aaaaacagaa ccctgggaag tgtttttatc gtggcgggaa
     2941 ccacaattgg cgcaggcatg ctggcaatgc cgctggctgc ggccggtgtt ggttttagcg
     3001 ttacgttaat cttgttgatt gggctttggg cgttgatgtg ctacacggcg ctattactgc
     3061 tggaggtgta ccagcatgtt ccggcagata ccggtctggg cacgctggca aaacgctatc
     3121 tgggacgcta cggtcaatgg ctgacgggct tcagtatgat gttcttaatg tatgctctga
     3181 ctgcagcata catcagcggt gccggtgaat tgttggcctc cagcatcagc gactggacag
     3241 gtatttcaat gtcggcaacc gctggcgtgc tgttgttcac ttttgttgcc ggtggcgtgg
     3301 tttgtgttgg aacatcgctg gtcgatttat ttaaccgttt tctgttcagc gccaaaatta
     3361 tttttctggt ggtgatgctg gtattgctgc taccgcatat tcacaaagtg aatcttttaa
     3421 ccctgccgtt gcaacagggg ctggcactgt ctgcaatccc ggtgattttt acgtcgtttg
     3481 gttttcacgg tagcgtgccg agtattgtca gctatatgga tggcaacatt cgtaagctac
     3541 gctgggtgtt tataatcggt agtgccatcc ccctggtggc atatattttc tggcaggtgg
     3601 cgacgcttgg cagcattgat tcaacaacct ttatgggatt gctggctaat catgctggat
     3661 taaacgggct gttacaggcg ttacgcgaaa tggtggcctc tccgcatgtt gagctggcgg
     3721 tgcatttatt tgctgattta gccctcgcca cgtcatttct cggcgttgcg ttaggcttat
     3781 ttgattatct ggctgatttg tttcagcgtt caaataccgt tggtggacga ttgcaaactg
     3841 gtgcaattac ctttctgccg ccgttggcgt ttgcactgtt ttatccacga ggatttgtga
     3901 tggcgctggg ttacgccggt gtggcgctgg cggtactggc attgattatc ccttcactat
     3961 tgacctggca aagcagaaag cacaatcctc aggcgggtta ccgggtcaaa ggtggtcgtc
     4021 cggcgctggt ggtggtgttt ctctgtggta ttgctgtgat tggcgtgcaa tttttgattg
     4081 cggcagggtt gttaccagaa gtggggtgat cagatagcct caaattcctt attgggtgcc
     4141 agaattaacg ctgacaccca atgtgacctc ttaatgcagg cagcactgct taaatttctt
     4201 accactgccg cacggacaag gatcgttacg ccccggtttc tcttctgctt tgatcggttg
     4261 ctgaacagct ttttcctgcg gatgcgccat ccagtatgca tgtaaatcaa gcgccgccag
     4321 tcgaatggca tctacgctct cttcaaacgc ttctggcgac attttctcta cccgctcgaa
     4381 gttttcctca gtaccgtgca gcgcaatcgc ctccagcgct ggttttaaag aatcgggcaa
     4441 cgttgaccag tcagaaagtg ccacgccccg catatagcca aagcaccact cctcaacaat
     4501 cgtcagctcg ctgccatcaa cttctcgcaa gccgaataac ggctcaaact gctccgggaa
     4561 ttcgttcaga cgctctgcgg tatcggccat atgttgaaaa gccaggttca taaagcgcgt
     4621 catctctttc tctgacgccc aacgcggcac atagtcagcc ccaccccaca cggcaaccag
     4681 ccactgttct ggttcaatct cttgcggaga actcaacacc gccgtcaata aaccgtccag
     4741 ctccgccaca tcaaggatgg cgtggtcagt gttgtatttg gtcagaatat cgtccagcca
     4801 ttccaactca ctttcgttta acggtcccgt tttcatacgc ttttccttgt ggatctcaac
     4861 tcgccagcac ctatcttaca tgccggtccg tatcagagat actttttgag tggctttgcc
     4921 ggtgattaaa aattaaggag ggtgtaacga caagttgcag gcacaaaaaa accacccgaa
     4981 ggtggtttca cgacactgct tattactttg attttattct tatctttccc atggtacccg
     5041 gagcgggact tgaacccgca cagcgcgaac gccgagggat tttaaatccc ttgtgtctac
     5101 cgattccacc atccgggctc gggaagaaag tggaggcgcg ttccggagtc gaaccggact
     5161 agacggattt gcaatccgct acataaccgc tttgttaacg cgccaaattc ttcaggcctt
     5221 tcagccagac atccgcttga cgccgatgtc ttttaaactg gagcgggaaa cgagactcga
     5281 actcgagacc tcgaccttgg caaggtcgtg ctctaccaac tgagctattc ccgcattcat
     5341 caagcaatca gttaatcact tgattttatt atcgtctggc aatcagtgcc gccgttcgat
     5401 gcgttgcatt ctacttacct ggcgcgatga gtcaacgata tttttcacca cttttgatcg
     5461 tttgctgaaa attacgccga aacgatcact gatcaagcaa atctgcacgc gcagcgctca
     5521 aatattgcaa cattgaccac agagtcagta ccgcagccac aaagaaaagt gcaataccga
     5581 cgtactcaac ccaaatgttc ggacgccaca gcagccaggc caacgccacc atctgggcag
     5641 tggttttcac tttcccaatc caggagacgg ccacgctact gcgtttaccc aactccgcca
     5701 tccattcgcg tagcgcagaa ataataattt cacgggcgat catcgttgcc gccggtaatg
     5761 tcacccacca gctgtgataa tgctcggtta ccagcaccat ggcgatagcc acgagaactt
     5821 tatctgccac agggtcaagg aaagcaccaa accgggtact ctggttccag cggcgtgcca
     5881 gaaaaccatc gaaccagtca gtcaccgccg cgacgcagaa aatgagcgcg gcggcaaacg
     5941 gcgaccaggt gacaggcaga taaaagacca atacaaagaa tgggataagg atgacacgga
     6001 acagtgtaag caacgtaggg atattaaatt gcataatgac gggtaactat ctgttgtcag
     6061 taagattacc cctatgttgc tacagagaca tcaatgtttc aacgaccaga agatcttttc
     6121 tgccagacct tgcgaaatac ccggcacttt tgcaatttcc tcgacgctgg cgttacgtaa
     6181 accttgcaaa ccgcccatat atttcaacaa catttgccga cgttttggcc cgacgccttc
     6241 aatggtttcc agggaactgg tatttttgac cttcgcccgt tttttacggt gcccgccaat
     6301 cgcgtgatca tgtgattcat cgcgaatatg ctggataaca tgcagcgcgg gtgaatctgg
     6361 cggcaaacta aatccctcac cttccggctc aaagaacagc gtttccagtc cagccttacg
     6421 atctgctcct ttggcaacgc caagtagcag cggatgattt ttatcccatg agacatccag
     6481 ttcggcgaag acatttttcg cctgcgcaag ctggcctttg ccgccgtcga taaggatcac
     6541 atccgggatc ttactgtcgt caatggcttt accataacgc cgacgcagca cctgattcat
     6601 cgccgcataa tcatcgcccg gcgtgatgcc tgtaatgtta tagcgccgat actccgcacg
     6661 cagcgggccg ttagcatcaa acaccacaca ggaagcgacg gtttgttcgc ccatggtatg
     6721 gctgatgtca aagcactcca tccgcttcac ttccggcaat ttcaacacgc tggcaagcgc
     6781 ggtcagtcgc tggtgaacgg tagattgctg cgaaagtttg ctggttaagg ccgtcgccgc
     6841 attggtgcgc gcgagtttca gataacgcgc cctatcgccg cgaggtttgg tttgaacatt
     6901 aatcttgcgt cccgccagtt ctgaaaggga atcggcgagc agcgttttat cgctaagatt
     6961 aaaatcgagc aggatctcac ccggtaaggt gcgcatctgg ctgccttgta aatagaactg
     7021 acctacgaag gtttctacca cctcgctcag ttccgtaccg ccaggcactt tcgggaaata
     7081 gctgcggctg ccgagcactt tgccctgacg aatgaacaat acgtggacac aagccatgcc
     7141 cgcatcgaac gccacaccaa taacgtcaag gtcgtcgccg gtattggaaa caaactgttt
     7201 ttcggtgacg cgtcgcaccg cctgaatttg gtcgcgaata catgccgctt cttcaaactc
     7261 cagattctgg ctggcagttt ccatacggct aatgagttgc gtaagcacct gatcatcttt
     7321 gccagacaaa aacaggcgca catactcgac ctgctgagcg tattcttctt cactcaccag
     7381 tccttcaacg cacggtccca gacagcgccc tatctggtat tgcagacacg gacgcgagcg
     7441 attgcgataa acactatttt cgcactggcg aatggggaaa atcttttgca gtagcgccag
     7501 tgtttcacgt acggcatagc cattcgggaa cgggccgaaa tattcacctt tggcatgctt
     7561 tgcaccacga tgcatcgcca gacgcgggtg ggtatcgcca ctcaggaaga taaaaggata
     7621 agatttatca tcgcgtagca aaacgttgta acgcggctga tagagtttga tgtagttgtg
     7681 ttccagcaac agcgcttcgg tttctgtatg cgtaaccgtt acatcaattt gctggatctg
     7741 ggcgaccagc gcttcggttt tgcgcgaagc gaggttgctg cggaaatagc tggaaagccg
     7801 ttttttcagg tctttcgctt tgccgacata gataaccgta ccaccagcat cgtacatgcg
     7861 ataaacgcct ggctggctgg ttacggtttt taaaaacgct tttgcgtcaa actgatcact
     7921 cactgacttg ataatgtctc cgcattacac agaccatggc gaattgccag gtgagtcagc
     7981 tcaacatcgc catgaatgtt tagtttactg aacatacgat agcggtagct gttcaccgtt
     8041 ttcggactga gattgagctg ttctgagatc tcattgacct tctggccctt agtgatcatc
     8101 agcataatct gcaattcacg ttcagacaaa ctggcaaatg ggctttctgt tttttctggt
     8161 tcgatctggc ttaacgccat ttgttgagcg atatcagaag caatgtaacg ctgccctgaa
     8221 tagacagaac gaatcgcact cacgacttcc tgcggagccg cgcctttgct gaggtagcca
     8281 gcagcacctg cctgcatgac tttcgctggt aaagggtttt ctgtatggac cgtaagcatg
     8341 atgattttaa catcagctgt ggaacgcgcg attttacgcg tcgcctcaag accgccaatg
     8401 cccggcatac tcatgtccat tagcaccacg tcaacggcat ttgtccggca ccacttaacg
     8461 gcgtcttcac cgcacgatgc ctctccgacg acttttatac ccttaatatc ttccagaatg
     8521 cgtcgtatcc ctgcgcgcac cagttcgtgg tcatcaacaa gtagaacgtt gatcaaagga
     8581 atatctccag aaatagggat aacgctactg atagttagtc attcgtatat tagcggtttt
     8641 tattgcaact ttgaaacgtt aaaaatggtg ggtattcgat ttttctctcg tttttgtcaa
     8701 ttcgattgtc cacagttatg gtcacgcccg tcaccaacgg cttaccggat cgtttcacag
     8761 taagccctac gatgaaatgt gacaaaaatt gactttattc agcaaaaatg aaaatcagcc
     8821 c
//
LOCUS       DAZXUK010000165         8722 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZXUK010000165 DAZXUK010000000
VERSION     DAZXUK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.165"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            31..903
                     /locus_tag="ACH3L8_002936"
     CDS             31..903
                     /locus_tag="ACH3L8_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFT4085964.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            1237..4359
                     /gene="agn43"
                     /locus_tag="ACH3L8_002937"
                     /gene_synonym="flu"
     CDS             1237..4359
                     /gene="agn43"
                     /locus_tag="ACH3L8_002937"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFT4085965.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            4480..6996
                     /locus_tag="ACH3L8_002938"
     CDS             4480..6996
                     /locus_tag="ACH3L8_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4085966.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            7072..7527
                     /locus_tag="ACH3L8_002939"
     CDS             7072..7527
                     /locus_tag="ACH3L8_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFT4085967.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            7670..7759
                     /locus_tag="ACH3L8_002940"
     CDS             7670..7759
                     /locus_tag="ACH3L8_002940"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFT4085968.1"
                     /translation="MTAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            7938..>8722
                     /locus_tag="ACH3L8_002941"
     CDS             7938..>8722
                     /locus_tag="ACH3L8_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFT4085969.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWV"
BASE COUNT         2070 a         2304 c         2454 g         1894 t
ORIGIN      
        1 gttttgtctt ttttcaccgg gaatacatgt atgaatccgt ctgatgccat tgaggcaatt
       61 gaaaaaccgc tctcctccct gccttactca ctttcccgtc acatcctgga acatctgcgc
      121 aaactcacca gtcacgaacc cgtgattggc attatgggta aaagcggggc cggtaaatct
      181 tcactctgta atgcactgtt tcagggggag gtcacaccgg tcagtgatgt tcacgccggc
      241 acccgggaag tgcagcgctt ccgtctgagt ggtcacggtc acagcatggt tatcactgac
      301 ctgcccgggg tgggcgagag ccgggacagg gatgcagagt atgaagccct gtaccgtgac
      361 attctgcctg aactggacct ggtgctgtgg ctgattaaag ccgatgaccg ggccctgtct
      421 gtggatgagt atttctggcg acacatcctg cagtgcggac atcagcaggt gctgtttgtg
      481 gtgacgcagg ctgacaaaac ggaaccctgc catgaatggg atatggcagg tattcagcct
      541 tctcccgcac aggaacagaa cattcgtgag aaaacggagg cggtattccg tctgttccgg
      601 cccgtacatc cggttgttgc cgtatcggcc cgcaccggct gggaactgga tacgctggtc
      661 agtgcgctca tgacagcgct tcccgaccat gccgccagcc ccctgatgac ccgactgcag
      721 gacgagctgc gcacggagtc tgtccgctct caggcccgtg aacagtttac cggtgcggtg
      781 gaccggatat ttgacacagc ggagagcgtc tgtgttgcct ctgttgtccg tacggccctg
      841 cgcgctgttc gtgacaccgt ggtctctgtt gcccgcgcgg tatggaactg gatcttcttc
      901 tgaacctgtt gtggatgatg tcctccctgc ctctgagtct gctcacaaaa gcgctgtttt
      961 cgttactgtc tctcttgtcc gtgcaataga ctgataatag aataaaacga tcgataccta
     1021 ttttaccgat cgttaatatc gatcgttatg ctaataataa ctcctgttag caacgtgcgc
     1081 agatacacac agacatgaga ttcagggaac aacagagcca cacgtcagaa acttccgtca
     1141 gccggacctc cggcactgta accctttacc tgccggtatc cacatctgtg gataccggct
     1201 tttttattca ccctcactct gattaaggaa atgctgatga aacgacatct gaatacctgc
     1261 tacaggctgg tatggaatca cattacgggc gctttcgtgg ttgcctccga actggcccgc
     1321 gcacagggta aacgtggcgg tgtggcggtt gcactgtctc ttgccgcggt cacgtcactc
     1381 ccggtgctgg ctgctgacat cgttgtgcac ccgggtgaaa cagtgaatgg cggaacactg
     1441 gtaaaccatg acaaccagtt tgtatccgga acagctgatg gcgtgactgt cagtaccggg
     1501 cttgagctgg ggccggacag tgacgaaaac accggcgggc aatggataaa agcgggtggc
     1561 acaggcagaa acaccactgt caccgcaaat ggtcgtcaga ttgtgcaggc aggaggaact
     1621 gccagtgata cggttattcg tgatggcgga gggcagagcc ttaacggact ggcggtgaac
     1681 accacgctgg ataacagagg tgagcagtgg gtacacgggg gagggaaagc tgccggtaca
     1741 attattaacc aggatggtta ccagaccata aaacatggcg gactggcaac cggaaccatc
     1801 gtcaacaccg gtgcagaagg tggtccggag tctgaaaatg tgtccagcgg tcagatggtc
     1861 ggagggacgg ctgaatccac caccatcaat aaaaatggcc ggcaggttat ctggtcttcg
     1921 gggatggcac gggacaccct catttacgcc ggtggtgacc agacggtaca cggagaggca
     1981 cataacaccc gactggaggg gggtaaccag tatgtacaca acggtggcac ggcaacagag
     2041 acgctgataa accgtgatgg ctggcaggtg attaaggaag gaggaactgc cgcgcatacc
     2101 accatcaacc agaaaggaaa gctgcaggtg aatgccggcg gtaaagcgtc tgatgtcacc
     2161 cagaacacgg gcggtgcact ggttaccagt acagctgcaa ccgtcaccgg cacaaaccgc
     2221 ctgggagcat tctctgttgt ggagggtaaa gctgataatg tcgtactgga aaatggcgga
     2281 cgcctggatg tgctgaccgg acacacagcc actaataccc gcgtggatga tggcggaacg
     2341 ctggatgtcc gcaacggtgg caccgccacc accgtatcca tgggaaatgg cggtgtactg
     2401 ctggccgatt ccggtgccgc tgtcagtggt acccggagcg acggaaaggc attcagtatc
     2461 ggaggcggtc aggcggatgc cctgatgctg gaaaaaggca gttcattcac gctgaacgcc
     2521 ggtgatacgg ccacggatac cacggtaaat ggcggactgt tcaccgccag gggcggcaca
     2581 ctggcgggca ccaccacgct gaataacggc gccatactta ccctttccgg gaagacggtg
     2641 aacaacgata ccctgaccat ccgtgaaggc gatgcactcc tgcagggagg cgctctcacc
     2701 ggtaacggca gcgtggaaaa atcaggaagt ggcacactca ctgtcagcaa caccacactc
     2761 acccagaaag ccgtcaacct gaatgaaggc acgctgacgc tgaacgacag taccgtcacc
     2821 acggatgtca ttgctcagcg cggtacagcc ctgaagctga ccggcagcac tgtgctgaac
     2881 ggtgccattg accccacgaa tgtcactctc gcctctggtg ccacctggaa tattcccgat
     2941 aacgccacgg tgcagtcggt ggtggatgac ctcagccatg ccggacagat tcatttcacc
     3001 tccacccgca cagggaagtt cgtaccggca accctgaaag tgaaaaacct gaacggacag
     3061 aatggcacca tcagcctgcg tgtacgcccg gatatggcac agaacaatgc tgacagactg
     3121 gtcattgacg gtggcagggc aaccggaaaa accatcctga acctggtgaa cgccggcaac
     3181 agtgcgtcgg ggctggcgac cagcggtaag ggtattcagg tggtggaagc cattaacggt
     3241 gccaccacgg aggaaggggc ctttatccag gggaataagc tgcaggccgg tgcctttaac
     3301 tactccctca accgggacag tgatgagagc tggtatctgc gcagtgaaaa tgcttatcgt
     3361 gcagaagtcc ccctgtatgc ctccatgctg acacaggcaa tggactatga ccggattctg
     3421 gcaggctccc gcagccatca gaccggtgta agcggtgaaa ataacagcgt ccgtctcagc
     3481 attcagggcg gtcatctcgg tcacgataac aacggcggta ttgcccgtgg agccacgccg
     3541 gaaagcagcg gcagctatgg cttcgtccgt ctggagggtg acctgctcag aacagaggtt
     3601 gccggtatgt ctgtgaccgc gggggtatat ggtgctgctg gccattcttc cgttgatgtt
     3661 aaggatgatg acggctcccg tgccggcacg gtccgggatg atgccggcag cctgggcgga
     3721 tacctgaatc tgatacacaa cgcctccggc ctgtgggctg acattgtggc ccagggaacc
     3781 cgccacagca tgaaagcctc atcggacaat aacgacttcc gcgtccgggg ctggggctgg
     3841 ctgggttcgc tggaaaccgg tctgcccttc agtatcactg acaatctgat gctggagccg
     3901 caactgcagt acacctggca gggactctcc ctggatgacg gccaggataa cgccagttat
     3961 gtgaagttcg ggcatggcag tgcacaacat gtgcgtgccg gcttccgtct gggcagccac
     4021 cacgatatga actttggtaa aggcacctca tcccgtgaca ccctgcgcgg cagtgccaaa
     4081 cacagtgtgc gtgaactgcc ggtgaactgg tgggtacagc cttctgttat ccgcaccttc
     4141 agctcccggg gggacatgag catgggtaca gccgcagccg gcagtaacat gacgttctca
     4201 ccgtcacaga atggtacgtc actggacctg caggccggac tggaagcccg tgtccgggaa
     4261 aatatcaccc tgggcgttca ggccagttat gcccacagca tcaacggcag cagcgctgaa
     4321 ggttataaca gtcaggccac actgaatgta accttctgat aattctgcat tgcctctctg
     4381 tggtcccggt catgatgacc gggacccgga cgggggaaaa cgtttcagtg ccacattcac
     4441 tggcattcac aataacatga tattcatcac ggagtgacta tgttacagat agtcggcgcg
     4501 ctgattctgc tgatcgcagg atttgccatt cttcgccttt tgttcagagc attaaccagc
     4561 acagcgtctg cgctggcagg gttcatattg ctgtgtctgt tcggtccggc tttactggct
     4621 ggctatatca ctgaacgcat aacccggtta ttccatattc gctggctggc aggcgtattt
     4681 ctgacgattg ccggaatgat catcagcttc atgtggggac ttgatggtaa acatatcgca
     4741 ctggaggctc atacttttga ctctgtaaaa tttattctga ccaccgctct cgccgctggt
     4801 ctgctggctc ttcccgtgca gataagaacc attcagcaga acgggctcac acctgaagat
     4861 atcagcaagg aaattaacgg gtattactgc tgtttttata ctgctttttt ccttatggcg
     4921 tgttctgcat acgcaccatt gatcgcattg cagttcgata tttcaccctc actgatgtgg
     4981 tggggcgggt tgttgtactg gctggctgca ttagtgacgc tgctatgggc ggccagccag
     5041 atccaggcgc tgaaaagact gaccagtgcc atcagccaga cactggaaga acaaccggtg
     5101 ctcaacagta aatcgtggct gagcagtttg caaaacgatt acagccttcc tgaaacgctg
     5161 acggagcgca tctggctgac actcatttca caacggattt cccggggaga actgagggaa
     5221 tttgaactgg cagacggaaa ctggttactg aacaatgcct ggtatgaaag aaacatggca
     5281 gggtttaacg aacagttgaa agagaacctg tcattcacac ctgatgaact gaaaacgctc
     5341 ttccggaacc gcctgaattt atcaccggaa gcgaatgacg attttctcga tcgttgcctg
     5401 gacggcggtg actggtaccc cttttcagaa ggccgccgtt ttgtatcatt ccaccacgtg
     5461 gatgagcttc gtatctgtgc ctcctgcggg ctgacagaag tacatcatgc cccggaaaat
     5521 cataagccgg ctccggaatg gtactgctcc tctctttgtc acgaaacaga aacactgtgt
     5581 caggacattt atgaacgttc ttacaccggt tttatttccg atgcaacggc gaatggtctg
     5641 attctcatga aactgccgga aacctggagt acaaatgaga aaatgtttgc ttccggaggg
     5701 cagggacatg ggtttgccgc tgaacgggga aaccatattg tcgacagagt ccgtctgaaa
     5761 aacgcacgga tcctcggtga taataatgcc agaaatggag cagacagact ggtcagcgga
     5821 acagaaatcc agacgaaata ttgttcaact gcagcccgta gcgtcggtgc ggcattcgac
     5881 ggacaaaacg gacagtatcg ttacatggga aatcatggtc ccatgcaact ggaagtcccc
     5941 cgtgatcagt atgccggcgc tgtggaaacc atgaagaata agatccgcga aggtaaagta
     6001 cccggtgtaa ccgatcccgc agaagcgtcc cggctgattc gtcggggaca tctgacttat
     6061 acccaggccc gtaatatcac ccggttcggg accatcgaat cggtcactta tgatattgcc
     6121 gaggggtcgg ttgtcagtct ggcggccgga gggatcagtt ttgccctgac ggcatcggtc
     6181 ttctggctca gcaccggcga tcgcgatgct gccctgcaga cagctgctgt ccaggcagga
     6241 aaaaccttca cccgcacact ggctgtctac gtcacaaccc agcaacttca ccggctcact
     6301 gttgttcagg gtatgctgaa gcatattgat ttttcgacgg ccagcccgac tgtccggcag
     6361 gcgcttcaga aggggaccgg tgcaggaaat atcagtgccc tgaacaaagt gatgaagggt
     6421 acgctggtga catctctggc actggtagct gtcacaacca gccctgacat gatcaaaatg
     6481 ttgcggggac ggatctccgg tgcgcagttc atcaggaatc ttgccgtggc atcttccggt
     6541 gtggcaggtg gtgctgtcgg gtcagtggcg ggcgggatat tgttcagtcc actgggacca
     6601 tttggtgcac tgacagggcg tgtggttggc ggtgttctgg ggggaatgat tgcctccgct
     6661 gtatcaggaa aaattgccgg agcgctggtt gaagaagatc gcgtcaaaat tctggcaatg
     6721 attcaggagc aggtgacatg gcttgccggc agtttcctgc tgaccggaca tgagattgaa
     6781 aatctgaacg cgaatctggc ccgtgttatc gatcagaatg ctctggagat cattttcgcc
     6841 gccggtatac aacaacgggc cgcgaccaat atgttaatca aaccactggt ggtcagtatc
     6901 atcaggcaac gccccgtcat ggaatatgat gcatcccatc tcggcaagat ggttaaccgg
     6961 ctggaagaag cattcccccc ggagctcccg gcataacgtg tgttctgctc cttttaggtt
     7021 ttatcacctg atttgccagt gtcatcgtca tgactcagag aggtaaatac catgattcac
     7081 ctgttcaaaa cctgcatgat taccgccttc attctggggt taacgtggtc tgccccactc
     7141 cgggcacagg atcaacgtta catcagtata cgcaatacag atacgatatg gctcccggga
     7201 aatatttgtg cttaccagtt ccggctggat aatggcggaa acggtgaagg atttggcccc
     7261 ctcaccatca ctctgcaact caaagacaaa tatggtcaga cgctggtgac cagaaaaatg
     7321 gaaacggaag cttttggtga cagtaatgcc acgcgaacca cagacgcatt tctggaaacg
     7381 gagtgcgtgg aaaatgtcgc cacaaccgaa atcattaaag caactgaaga aagtaacggc
     7441 catcgtgtca gtctgccgtt atcggttttc gatccccagg actaccatcc actgctgatt
     7501 accgtttccg gaaaaaatgt taactgatcc ctgaagtcag tttcatgctt cagcctctgc
     7561 cacaacgtat ggcagaggct atttatcagg agaaaccaga tatgccgggt tacacacaga
     7621 gtatgtcctg gctgaaggat ctttctcata cgggcaggcg gttgctgtca taaccgctta
     7681 ccgcaatgtg tttatccagg atgacccggg aatgcatttc cgtcgggtta tccgcaacgc
     7741 ggaaggtcag cgacgctgac gcagccggaa ctctgaagct gatgccggaa aacagcttaa
     7801 tgcgtggctc gccagtggag ggcttctcag acaataaact tcattgattt catccatcag
     7861 gccgcgtctt ctccgggaga cgcggctttt ttcatttgtt gcaccagtaa atcttaacca
     7921 ccgataagga gcaaagtatg caattagtca gtcgttttgg ttatgcgaac cagatacgcc
     7981 gtgaccgtcc gctgacacat gaagagctga tgcaccatgt gccggggatt ttcggggaag
     8041 agaaacacac gtcccgaagc cagaactata cgtacatccc caccatcacc gtactggaaa
     8101 gtctgcagcg ggaaggcttc cagccctttt tcgcctgcca gacccgtgtg cgcgacccgg
     8161 gccgccgggg atacacaaaa cacatgctgc gtctgcggcg ggccggagag ataaacggag
     8221 aacatgtccc tgaaattatt ctgctcaact cccatgacgg tacctccagc taccagatgc
     8281 tgccgggtta cttcagattc gtctgccaga acgggtgtgt ctgtggtcag tctctggggg
     8341 aagtgcgtgt tccgcaccgg ggaaacgtgg tggagaaagt tattgaaggg gcttacgaag
     8401 tggtgggcgt gtttgaccgg attgaggaga aacgtgatgc catgcagtcg ctggtcctgc
     8461 cgccaccggc acgccaggcg ctggcacagg cggcactgac ttaccgttat ggtgacgaac
     8521 atcagcccgt caccaccgcc gacattctga cgccacgacg ccgggaggat tacggtaagg
     8581 acctgtggag tacttatcag accatccagg agaatatgct gaaaggcggg atttccggcc
     8641 gcagtgcaaa aggaaaacgt atccacaccc gtgccattca caacatcgac accgatatta
     8701 agctcaaccg cgcattgtgg gt
//
LOCUS       DAZXUK010000166         8675 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZXUK010000166 DAZXUK010000000
VERSION     DAZXUK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.166"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..823
                     /gene="odhB"
                     /locus_tag="ACH3L8_002942"
     CDS             <1..823
                     /gene="odhB"
                     /locus_tag="ACH3L8_002942"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFT4085970.1"
                     /translation="KGTGVGGRLTREDVEKHLAKAPAKESAPAAAAPAAQPALAARSE
                     KRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGF
                     MSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGM
                     ADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHA
                     IKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEEPTRLLLDV"
     gene            917..2083
                     /gene="sucC"
                     /locus_tag="ACH3L8_002943"
     CDS             917..2083
                     /gene="sucC"
                     /locus_tag="ACH3L8_002943"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFT4085971.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            2083..2952
                     /gene="sucD"
                     /locus_tag="ACH3L8_002944"
     CDS             2083..2952
                     /gene="sucD"
                     /locus_tag="ACH3L8_002944"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFT4085972.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(3055..3777)
                     /gene="mngR"
                     /locus_tag="ACH3L8_002945"
     CDS             complement(3055..3777)
                     /gene="mngR"
                     /locus_tag="ACH3L8_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFT4085973.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            3947..5863
                     /gene="mngA"
                     /locus_tag="ACH3L8_002946"
     CDS             3947..5863
                     /gene="mngA"
                     /locus_tag="ACH3L8_002946"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFT4085974.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            5881..8514
                     /gene="mngB"
                     /locus_tag="ACH3L8_002947"
     CDS             5881..8514
                     /gene="mngB"
                     /locus_tag="ACH3L8_002947"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFT4085975.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         2131 a         2092 c         2397 g         2055 t
ORIGIN      
        1 taaaggcacc ggtgtgggtg gtcgtctgac tcgtgaagat gtggaaaaac atctggcgaa
       61 agccccggcg aaagagtctg ctccggcagc ggctgctccg gcggcgcaac cggctctggc
      121 tgcacgtagt gaaaaacgtg tcccgatgac tcgcctgcgt aagcgtgtgg cagagcgtct
      181 gctggaagcg aaaaactcca ccgccatgct gaccacgttc aacgaagtca acatgaagcc
      241 gattatggat ctgcgtaagc agtacggtga agcgtttgaa aaacgccacg gcatccgtct
      301 gggctttatg tccttctacg tgaaagcggt ggttgaagcc ctgaaacgtt acccggaagt
      361 gaacgcgtct atcgacggcg atgacgtggt ttaccacaac tatttcgacg tcagcatggc
      421 ggtttctacg ccgcgcggcc tggtgacgcc ggttctgcgt gatgtcgata ccctcggcat
      481 ggcagacatc gagaagaaaa tcaaagagct ggcagtcaaa ggccgtgatg gcaagctgac
      541 ggttgaagat ctgaccggtg gtaacttcac catcaccaac ggtggtgtgt tcggttccct
      601 gatgtctacg ccgatcatca acccgccgca gagcgcaatt ctgggtatgc acgctatcaa
      661 agatcgtccg atggcggtga atggtcaggt tgagatcctg ccgatgatgt acctggcgct
      721 gtcctacgat caccgtctga tcgatggtcg cgaatccgtg ggcttcctgg taacgatcaa
      781 agagttactg gaagagccga cgcgtctgct gctggacgtg tagtagttta agcttcacct
      841 gcactgtaga ccgacgtgga tagggtgtcg gtctacggtt taaaagataa cgattactga
      901 aggatggaca gaacacatga acttacatga atatcaggca aaacaactgt ttgcccgcta
      961 tggcttacca gcaccggtgg gttatgcctg tactactccg cgcgaagcag aagaagccgc
     1021 ttcaaaaatc ggtgccggtc cgtgggtagt gaaatgtcag gttcacgctg gtggccgcgg
     1081 taaagcgggc ggtgtgaaag ttgtaaacag caaagaagac atccgtgctt ttgcagaaaa
     1141 ctggctgggc aagcgtctgg taacgtatca aacagatgcc aatggccaac cggttaacca
     1201 gattctggtt gaagcggcga ccgatatcgc taaagagctg tatctcggtg ccgttgtaga
     1261 ccgtagttcc cgtcgcgtgg tctttatggc ctccaccgaa ggcggcgtgg aaatcgaaaa
     1321 agtggcggaa gaaactccgc acctgatcca taaagttgcg cttgatccgc tgactggccc
     1381 gatgccgtat cagggacgcg agctggcgtt caaactgggt ctggaaggta aactggttca
     1441 gcagttcacc aaaatcttca tgggcctggc gaccattttc ctggagcgcg acctggcgct
     1501 gatcgaaatc aacccgctgg tcatcaccaa acagggcgat ctgatttgcc tcgacggcaa
     1561 actgggcgct gatggcaacg ccctgttccg ccagcctgat ctgcgcgaaa tgcgtgacca
     1621 gtcgcaggaa gatccgcgtg aagcacaggc tgcacagtgg gaactgaact acgttgcgct
     1681 ggacggtaac atcggttgta tggttaacgg cgcaggtctg gcgatgggta cgatggacat
     1741 agttaaactg cacggcggcg aaccggctaa cttcctggac gttggcggcg gcgcaaccaa
     1801 agaacgtgta accgaagcgt tcaaaatcat cctctctgac gacaaagtga aagccgttct
     1861 ggttaacatc ttcggcggta tcgttcgttg cgacctgatc gctgacggta tcatcggcgc
     1921 ggtagcggaa gtgggtgtta acgtaccggt cgtggtacgt ctggaaggta acaacgccga
     1981 actcggcgcg aagaaactgg ctgacagcgg cctgaatatt attgcagcaa aaggtctgac
     2041 ggatgcagct cagcaggttg ttgccgcagt ggaggggaaa taatgtccat tttaattgat
     2101 aaaaacacca aggttatctg ccagggcttt accggtagcc aggggacttt ccactcagaa
     2161 caggccattg catacggcac taaaatggtt ggtggcgtaa ctccaggtaa aggcggcact
     2221 acccacctcg gcctgccggt gttcaacacc gtgcgtgaag ctgttgctgc tactggcgct
     2281 tccgcgtctg ttatttacgt accagcaccg ttctgcaaag actccattct ggaagctatc
     2341 gatgcaggca tcaaactgat tatcaccatc actgaaggca tcccgacgct ggatatgctg
     2401 accgtgaaag tgaaactgga cgaagcaggc gttcgtatga tcggcccgaa ctgcccaggc
     2461 gttatcaccc cgggtgaatg caaaattggt atccagccgg gtcacattca caaaccgggt
     2521 aaagtgggta tcgtttcccg ttccggtaca ctgacctatg aagcggttaa acagaccacg
     2581 gattacggtt tcggtcagtc gacctgtgtc ggtatcggcg gtgacccgat ccctggctct
     2641 aactttatcg acattctcga aatgttcgaa aaagatccgc agaccgaagc gatcgtgatg
     2701 atcggtgaga tcggcggtag cgctgaagaa gaagcggcag cgtacatcaa agagcatgtt
     2761 accaagccag ttgtgggtta catcgctggt gtgactgcgc cgaaaggcaa acgtatgggc
     2821 cacgcgggtg cgatcattgc cggtgggaaa gggactgcgg atgagaaatt cgctgctctg
     2881 gaagccgcag gcgtgaaaac cgttcgcagc ctggcggata tcggtgaagc actgaaaact
     2941 gttctgaaat aaatatctgt aataagaaat agccctcgcc gcttccctct acaggaatgg
     3001 cgaagggctg tcggtttcga catggttggc catcgtatga tggccttttt gtgcttatcg
     3061 cgatgatttt cgctgcgcta tcagggtaaa tttatagtca tcggtattaa aagcgttgcg
     3121 gctatattca aacacccgac catcaactaa atatccacgc gatacttttt caagaatcgg
     3181 ctttgtctgg ctgatattaa gcagacggct catctcttcg gttggcatca gaggaatgat
     3241 ttcctgttcg ctacgatcga taaccatctt cttcacttct tcaataaagt gatatttcga
     3301 attttccatg acctgccagg tgagatccgg gaacaacgca agcggcatcc aggtttcttc
     3361 cagcgccatt ggcttttgct tgcgatagcg cacgcgcttc acatgccaaa cacgatcctg
     3421 cacggtgatt tgtagctgtt gctgaagaaa atcgtcagcc ggaatcactt caaatatcag
     3481 aacttcactg tgtgtatcga cgtgacggtc cgacagtttt tcatcaaaac tggttaactg
     3541 aaaaatatcg taattgaccc gctcttcttt gacgtaagtc ccgctgccct gaatgctttc
     3601 gaggatctgt tgctcgacta actggcgcaa agcctgacgc accgtaaccc ggctgacgcc
     3661 aaactctgtt tgtaatgccg attcggtagg taacgcatcg ccaggtttaa gctcgccacg
     3721 cgcaatttgc tcacgaatgc gatcagcaat ctgtcggtat aagggtttgt gtcccattat
     3781 ttagcatctc attaatacga atttagccat tatgcccgat aaactcatcc tgtaaacaat
     3841 acaaataaaa tgcaaataat ttcaatcaag tgaaattgat tacgcaatgg tattgttttg
     3901 tcgggcacac tggcgcgact ataaaaacga tcaagtgagg atcatgatga acctgacgac
     3961 tctgacccac cgcgatgcgt tgtgtctgaa tgcgcgcttt accagccgtg aagaggccat
     4021 tcacgcgttg actcaacgtc ttgctgctct ggggaaaatt tccagtactg agcaatttct
     4081 gaaagaagtg tatcgccgtg aaagccttgg cccgaccgcc ttaagtgaag ggttggctgt
     4141 gccgcatggc aaaactgctg cggtaaaaga agcggcgttt gcggtcgcca cactcagcga
     4201 gccgcttcag tgggaaggcg tggatggccc ggaagcggtt gatttagtgg tgttgctggc
     4261 gattcccctc aatgaagcgg gtactacgca catgcaactg ctgacagcgc tgaccacgcg
     4321 cctggcggat gatgagattc gggcgcgtat acagtcggcg acgacgcctg atgaattgct
     4381 ctcggcgctg gatgacaagg gaggcacgca accttctgcc tctttttcca acgcgccaac
     4441 tatcgtttgc gtaacggcct gcccggcggg tattgctcac acctacatgg ctgcggagta
     4501 tctggaaaaa gccggacgca aactcggcgt gaatgtctac gttgaaaagc aaggtgctaa
     4561 cggtattgaa gggcgtttaa cggcggatca actcaatagt gcaactgcct gtatttttgc
     4621 ggctgaagtc gccatcaagg agagtgagcg ttttaacggc attcctgcgc tttctgtgcc
     4681 tgttgccgag ccgattcgcc atgcagaagc gttgatccaa caatcgctta ccctcgagcg
     4741 tggcgatgag acgcgtacct tacagcaaga tacgcaaccg gtgaaaagcg tcaaaacgga
     4801 gctgaaacag gcactgttga gcgggatctc ttttgccgta ccgttgattg tcgcgggggg
     4861 gacggtgctg gcggtcgcgg tattactgtc gcaaatcttc gggctacaag atctgtttaa
     4921 tgaagaaaac tcctggctgt ggatgtaccg caaactgggc ggcgggctgc tcggaatttt
     4981 gatggtaccg gtgctggcag cctataccgc ctattcgctg gcagataaac cggcgttagc
     5041 gccaggcttt gcggctggac ttgccgccaa catgatcggc tccgggtttc tcggcgcggt
     5101 cgttggcgga ttgatagccg gttacttgat acgctggatg aaaaatcact tgcgtcttag
     5161 cagtaaattc aatggcttcc tgacttttta tctctacccg gtgctcggta cattgggagc
     5221 gggcagtctg atgctgtttg tggtggggga acctgtcgcc tggatcaata actcgcttac
     5281 cgcctggctg aacggtctgt caggaagtaa cgcgctgttg ctgggtgcca ttctcggttt
     5341 tatgtgttcc tttgaccttg gagggccagt gaataaagcc gcttatgcat tctgcctggg
     5401 cgcaatggcg aacggcgttt acggcccgta tgccattttc gcctccgtca aaatggtttc
     5461 ggcatttacc gtaaccgctt ccacgatgct cgcaccgcgc ctgtttaaag agtttgaaat
     5521 tgagaccggg aaatccacct ggctgttagg gctggcaggt attaccgaag gggcgatccc
     5581 gatggcgatt gaagatccgc tgcgggttat tggttcgttt gtgctgggct ctatggtaac
     5641 gggcgctatt gtcggtgcga tgaatatcgg cctttcgaca cccggtgctg gcattttctc
     5701 gctcttttta cttcatgata atggcgcggg cggtgttatg gcggcaattg gctggtttgg
     5761 tgcggcattg gtgggggctg caatctcgac tgcaattctc ctgatctggc ggcgtcacgc
     5821 ggttaagcat ggcaactatc tgactgatgg cgtaatgcca taaacaaaaa ggaaacgacg
     5881 atgaaagcgg tatctcgcgt tcacatcacc ccgcatatgc actgggatcg ggagtggtat
     5941 ttcaccaccg aagagtcacg cattctgctg gtcaataata tggaagagat cctgtgccga
     6001 ctggaacagg acaacgaata caaatattac gtactcgacg ggcaaacggc gatcctcgaa
     6061 gattatttcg cggtgaaacc ggaaaacaaa gaccgcttga agaaactggt agaagccggc
     6121 aagttgatta tcggcccctg gtatacccag accgatacca cgattgtttc tgcggaatcc
     6181 atcgtccgta atctgatgta cggaatgcgt gactgcctcg cgtttggcga gccgatgaaa
     6241 ataggttatt tacccgattc ctttggcatg tccgggcaac tgccgcatat ctacaatgga
     6301 tttggcatta cctgcaccat gttctggcgt ggatgttctg agcgccacgg tacggataaa
     6361 accgagtttt tgtggcaaag cagtgacggt agcgaagtga cggcgcaggt gatgccgttg
     6421 ggttatgcca tcggtaagta cttacctgcg gacgaaaacg gattacgcaa acgcctcgac
     6481 agttattttg acgtgctgga aaaagcgtct gtaaccaaag agattttgct gccgaatggg
     6541 catgatcaga tgccattgca gcaaaatatc ttcgaagtga tggagaaact gcgcgaaatt
     6601 tatcctcaac gaaagtttgt gatgagccgc tttgaagagg tatttgagaa gatcgaagcg
     6661 cagcgagata atctggcaac cctgaaaggg gaatttattg atggcaaata tatgcgcgtg
     6721 catcgcacca tcggttccac gcgcatggat atcaaagttg cccacgcacg tattgaaaat
     6781 aagattgtta atctgctgga accgctggca acgctggcca ggacgttggg ttttggatac
     6841 caccacggct tgctggagaa aatgtggaaa gagatcttaa aaaatcatgc ccacgacagc
     6901 atcggctgct gctgtcgtga caaagttcat cgcgaaatcg tcgcccgctt cgaactggct
     6961 gaagacatgg cggataatct gctgcgtttc tatatgcgta aaatcgccga caacatgccg
     7021 cagagcgacg ccgacaaact cgtcctgttt aacctgatgc cctggccgcg ggaagaagtg
     7081 atcaacacca ctgtgcggct gcgcgccagc cagtttaatc tacgagacga tcgcggtcag
     7141 cctgtaccgt attttattcg ccatgcccgt gagatcgatc caggcctaat cgatcggcaa
     7201 atagttcatt acggtaatta cgatcccttt atggagtttg atatacagat caaccagatt
     7261 gtcccttcta tgggctatcg cacgctttat atcgaagcga atcagcctgg caacgtgatt
     7321 gcggcaaaaa gtgacgctga agggatactg gaaaatgctt tctggcaaat tgcgctcaat
     7381 gaggatggaa cgctacaact ggtagataaa gatagcggtg tgcgctatga ccgggtgttg
     7441 caaattgaag aaagctctga tgatggtgat gaatatgact attcacccgc aaaagaagag
     7501 tgggtaatta cttcggcgaa tgcgaaaccg caatgcgaga ttattcatga ggcctggcag
     7561 agcagggctg ttatccgcta tgaaatggcg gtgccgcgta atctgcaaca acgcagcgca
     7621 agacaatcca ctggcagagt agatgtcgag ttggttgtca ctcttagtca taacaacagg
     7681 cgcattgatg tggatatcaa ccttgataac caggctgacg atcatcgcct tcgcgtcctg
     7741 atccctacat cttttaacac cgatagtgtt ctggcagata cccagtttgg ttcgctaacg
     7801 cgccccgtga acgacagtgc aatgaacaac tggcagcaag aaagctggaa agaagcgccg
     7861 gttccggtat ggaatatgct caactatgca gccttacagg aagggcgtaa cggcatggct
     7921 gtctttagcg aagggctacg tgaatttgaa gtcatcggtg aagagaagaa aacctttgcc
     7981 attacgttgc tgcgcggcgt gggcttactg gggaaagaag atctgctttt aaggcctagg
     8041 cggccttcgg gaattaaaat gccagtcccg gactcacaac tacgtggtct gctttcttgt
     8101 cgcctaagtt tattgagtta taccggtacg ccaaccgccg ctggtgtagc tcagcaggcg
     8161 cgagcatggc tgactccagt acagtgttac aacaaaatcc catgggatgc gatgaagctc
     8221 aacaaagccg gattcaacgt gccggaaagt tatagtttgt tgaaaatgcc cccagtggga
     8281 tgcctgataa gcgcacttaa gaaagctgaa gaccgacaag aagtgatttt acggctgttt
     8341 aatccggctg aatcagcaac ctgtgatgcg actgttgctt tcagtcgcga ggtgatttct
     8401 tgctcagaaa cgatgatgga tgaacacatt accaccgagg aaaatcaagg ttcaaatcta
     8461 tcggggcctt ttttacccgg ccagtcacgg acgttcagtt accggcttgc ctgaatagca
     8521 atcaaaccga agccacatat gcgcggccag attgttgaca aagggcgctt tgttcatgcc
     8581 ggatacggca tgaacgcttt attcggtcta caaaagcagg caaattcaat atattgcaga
     8641 gatgatgtag acactggcaa gcgtagcgca tcagg
//
LOCUS       DAZXUK010000167         8669 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZXUK010000167 DAZXUK010000000
VERSION     DAZXUK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.167"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(329..592)
                     /gene="rpsT"
                     /locus_tag="ACH3L8_002948"
     CDS             complement(329..592)
                     /gene="rpsT"
                     /locus_tag="ACH3L8_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFT4085976.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            695..913
                     /locus_tag="ACH3L8_002949"
     CDS             695..913
                     /locus_tag="ACH3L8_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFT4085977.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            921..1862
                     /gene="ribF"
                     /locus_tag="ACH3L8_002950"
     CDS             921..1862
                     /gene="ribF"
                     /locus_tag="ACH3L8_002950"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFT4085978.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1905..4721
                     /gene="ileS"
                     /locus_tag="ACH3L8_002951"
     CDS             1905..4721
                     /gene="ileS"
                     /locus_tag="ACH3L8_002951"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFT4085979.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4721..5215
                     /gene="lspA"
                     /locus_tag="ACH3L8_002952"
     CDS             4721..5215
                     /gene="lspA"
                     /locus_tag="ACH3L8_002952"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFT4085980.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5302..5751
                     /gene="fkpB"
                     /locus_tag="ACH3L8_002953"
     CDS             5302..5751
                     /gene="fkpB"
                     /locus_tag="ACH3L8_002953"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFT4085981.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5753..6703
                     /gene="ispH"
                     /locus_tag="ACH3L8_002954"
                     /gene_synonym="lytB"
     CDS             5753..6703
                     /gene="ispH"
                     /locus_tag="ACH3L8_002954"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFT4085982.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6769..7683
                     /gene="rihC"
                     /locus_tag="ACH3L8_002955"
     CDS             6769..7683
                     /gene="rihC"
                     /locus_tag="ACH3L8_002955"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFT4085983.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7850..>8669
                     /gene="dapB"
                     /locus_tag="ACH3L8_002956"
     CDS             7850..>8669
                     /gene="dapB"
                     /locus_tag="ACH3L8_002956"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFT4085984.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
BASE COUNT         1980 a         2157 c         2461 g         2071 t
ORIGIN      
        1 ggtctaatga ggagatattt atgaatggtt aaagtatata ggaatttaat tcaaaaatag
       61 agtggtctta agtttaatac ctcaatgtgt atcacaatat ccatattctt tgtgggggag
      121 tctggagatt gagtagatat tcttgttcag aatgtatcag ccgatggttc tacgattctt
      181 aagccacgaa gagttcagat agtacaacgg catgtctctt ttgactatct ggcaaccggc
      241 agtgtgttct ctcacgcatc acaaaagcag caggcataaa aaaacccgct tgcgcgggct
      301 ttttcacaaa gcttcagcaa attggcgatt aagccagttt gttgatctgt gcagtcaggt
      361 tagccttatg acgtgcagct ttgtttttgt ggatcagacc tttagcagcc tgacggtcca
      421 cgatcggttg catttcgtta aatgctttct gtgcagcagc tttgtcgcca gcttcgatag
      481 ctgcgtatac tttcttgatg aaagtacgca tcatagagcg acggcttgcg ttgtgcttac
      541 gagccttttc agactgaatg gcgcgcttct tagctgattt gatattagcc aaggtccaac
      601 tcccaaatgt gttctatatg gacaattcaa aggccgagga atatgccctt ttagccttct
      661 tttgtcaatg gatttgtgca aataagcgcc gttaatgtgc cggcactcgt tacgtagtga
      721 tggcgcagga ttctaccagc ttgcggggtg tgaatacagc ttttccgcga taaaaattgc
      781 agcaggcggt cagtttcttc ccgtgatttg ctccatggca atgaaaagcc acttctttct
      841 gatttcggta ctcaatcgcc ggttaacctt gaccgctgta caaggtatac tcggacgatt
      901 ttcactgttt tgagccagac atgaagctga tacgcggcat acataatctc agccaggccc
      961 cgcaagaagg gtgtgtgctg actattggta atttcgacgg cgtgcatcgc ggtcatcgcg
     1021 cgctgttaca gggcttgcag gaagaagggc gcaagcgcaa cttaccggtg atggtgatgc
     1081 tttttgaacc tcaaccactg gaactgtttg ctaccgataa agccccggca agactgaccc
     1141 ggctgcggga aaaactgcgt taccttgcag agtgtggcgt tgattacgtg ctgtgcgtgc
     1201 gtttcgacag gcgttttgcg gcgttaaccg cgcaaaattt catcagcgat cttttggtga
     1261 agcatttgcg cgtaaaattt cttgccgtag gtgatgattt ccgctttggc gctggtcgtg
     1321 aaggcgattt cttgttatta cagaaagctg gcatggaata cggcttcgat atcaccagta
     1381 cgcaaacttt ttgcgaaggt ggtgtgcgta tcagcagcac cgccgtgcgt caggcgctgg
     1441 ctgatgacaa tctggctctg gcagaaagtt tactggggca cccgtttgcc atctccgggc
     1501 gtgtagtcca cggtgatgaa ttagggcgca ctataggttt cccgacggcg aatgtaccgc
     1561 tacgccgtca ggtttccccg gtgaaagggg tttatgcggt agaagtgctg ggcctcggtg
     1621 aaaagccgtt acccggcgtg gcaaacatcg gaacacgccc aacggttgcc ggtattcgcc
     1681 agcagctgga agtgcatttg ttagatgttg caatggacct ttacggtcgc catatacaag
     1741 tagtgctgcg taaaaaaata cgcaatgagc agcgatttgc gtcgctggac gaactgaaag
     1801 cgcagattgc gcgtgatgaa ttaaccgccc gcgaattttt tgggctaaca aaaccggctt
     1861 aagcctgtta tgtaatcaaa ccgaaacacg gaaccgagaa tctgatgagt gactataaat
     1921 caaccctgaa tttgccggaa acagggttcc cgatgcgtgg cgatctcgcc aagcgcgaac
     1981 cgggaatgct ggcgcgttgg actgatgatg atctgtacgg catcatccgt gcggctaaaa
     2041 aaggcaaaaa aaccttcatt ctgcatgatg gccctcctta tgcgaatggc agcattcata
     2101 ttggtcactc ggttaacaag attctgaaag acattatcgt gaagtccaaa gggctttccg
     2161 gttatgactc gccgtatgtg cctggctggg actgccacgg tctgccgatc gagctgaaag
     2221 tagagcaaga atacggtaag ccgggtgaga aattcaccgc cgccgagttc cgcgccaagt
     2281 gccgcgaata cgcggcgacc caggttgacg gtcaacgcaa agactttatc cgtctgggcg
     2341 tgctgggcga ctggtcgcac ccgtatctga ctatggactt caaaactgaa gccaacatca
     2401 tccgcgcgct gggcaaaatc atcggcaatg gtcacctgca caaaggcgcg aagccggtgc
     2461 actggtgcgt agactgccgt tctgcactgg cagaagcgga agttgagtat tacgacaaaa
     2521 cttctccgtc cattgacgtc gctttccagg cggtcgatca ggatgcgctg aaagcgaaat
     2581 ttggcgtaag caacgttaac ggcccaatct cgctggtgat ctggaccact acgccgtgga
     2641 ctctgcctgc gaaccgcgca atctctattg cacctgattt cgactatgcg ctggtgcaga
     2701 tcgacggtca ggccgtgatt ctggcgaaag atctggttga aagcgtaatg cagcgtatcg
     2761 gcgtgaccga ttacaccatt ctcggcacgg taaaaggtgc agagcttgag ctgctgcgct
     2821 ttacccatcc gtttatgggc ttcgacgttc cggcaatcct cggcgatcac gttaccctgg
     2881 atgccggtac cggtgccgtt cacaccgcgc ctggccacgg cccggacgac tatgtgatcg
     2941 gtcagaaata cggcctggaa accgctaacc cggttggccc ggacggcact tatctgccgg
     3001 gcacttatcc gacgctggat ggcgtgaacg tcttcaaagc gaacgacatc gtcgttgcgc
     3061 tgctgcagga aaaaggcgcg ctgctgcacg ttgagaaaat gcagcacagc tatccgtgct
     3121 gctggcgtca caaaacgccg atcatcttcc gcgcgacacc gcagtggttc gtcagcatgg
     3181 atcagaaagg tctgcgtgcg cagtcactga aagagatcaa aggcgtgcag tggctcccgg
     3241 actggggcca ggcgcgtatc gagtcgatgg ttgctaaccg tcctgactgg tgtatctccc
     3301 gtcagcgcac ctggggcgta ccgatgtcac tgttcgtgca caaagacacg gaagagctgc
     3361 atccgcgtac cctcgaactg atggaagaag tggctaaacg cgttgaagtt gacggcatcc
     3421 aggcgtggtg ggatctcgat gcgaaagaga tcctcggcga cgaagctgac cagtatgtga
     3481 aagtgccgga tactctcgac gtatggtttg actccggatc tacccactct tctgttgttg
     3541 acgtgcgtcc ggaatttgcc ggtcacgcag cggacatgta tctggaaggt tctgaccagc
     3601 accgtggttg gttcatgtct tccctaatga tctccaccgc gatgaaaggc aaagcgccgt
     3661 atcgtcaggt actgacccac ggctttaccg tggatggtca gggtcgcaag atgtctaaat
     3721 ccatcggcaa caccgtttcg ccgcaggatg tgatgaacaa actgggcgcg gatattctgc
     3781 gtctgtgggt ggcatcaacc gactacaccg gtgaaatggc cgtttctgac gagatcctga
     3841 aacgtgctgc cgatagctat cgtcgtatcc gtaacaccgc gcgcttcctg ctggcaaacc
     3901 tgaatggttt tgatccagca aaagatatgg tgaaaccgga agagatggtg gtactggatc
     3961 gctgggccgt aggttgtgcg aaagcggcac aggaagacat cctcaaggcg tacgaagcat
     4021 acgatttcca cgaagtggta cagcgtctga tgcgcttctg ctccgttgag atgggttcct
     4081 tctacctcga catcatcaaa gaccgtcagt acaccgccaa agcggatagc gtggcgcgtc
     4141 gtagctgcca gactgcgctg tatcacatcg ctgaagcgct ggtgcgctgg atggcaccaa
     4201 tcctctcctt caccgctgat gaagtgtggg gctatctgcc gggcgaacgt gaaaaatacg
     4261 tcttcaccgg tgagtggtac gaaggcctgt ttggtctggc agacagtgaa gcaatgaacg
     4321 atgcgttctg ggacgagctg ttgaaagtgc gtggcgaagt gaacaaagtc attgagcaag
     4381 cgcgtgccga caagaaagtg ggtggctcgc tggaagcggc agtaaccttg tatgcagaac
     4441 cggaactggc ggcgaaactg accgcgctgg gcgatgaatt acgatttgtc ctgttgacct
     4501 ccggcgctac cgttgcagac tataacgacg cacctgctga tgctcagcag agcgaagtac
     4561 tcaaagggct gaaagtcgcg ttgagtaaag ccgaaggtga gaagtgccca cgctgctggc
     4621 actacaccca ggatgtcggc aaggtggcgg aacacgcaga aatctgcggc cgctgtgtca
     4681 gcaacgtcgc cggtgacggt gaaaaacgta agtttgcctg atgagtcaat cgatctgttc
     4741 aacagggcta cgctggctgt ggctggtggt agtcgtgctg attatcgatc tgggcagcaa
     4801 atacctgatc ctccagaact ttgctctggg ggatacggtc ccgctgttcc cgtcgcttaa
     4861 tctgcattat gcgcgtaact atggcgcggc gtttagtttc cttgccgata gcggcggctg
     4921 gcagcgttgg ttctttgccg gtattgcgat tggtattagc gtgctcctgg cagtgatgat
     4981 gtatcgctcg aaggccacgc agaagctaaa caatatcgct tacgcgctga ttattggcgg
     5041 cgcgctgggc aacctgttcg accgcctgtg gcacggcttc gttgtcgata tgatcgactt
     5101 ctacgtcggc gactggcact tcgccacctt caaccttgcc gatactgcca tttgtgtcgg
     5161 tgcggcactg atcgtgctgg aagggttttt accttccaaa gcgaaaaaac aataataaat
     5221 cctgccggat gcggcctaca gattgctacg aaatcgtagg ccgcatccgg caaaaatcct
     5281 caaaaataag agcaaacctg catgtctgaa tctgtacaga gcaatagcgc cgtcctggtg
     5341 cacttcacgc taaaactcga cgatggcacc accgccgagt ctactcgcaa caacggtaaa
     5401 ccggcgctgt tccgcctggg cgatgcttct ctttctgaag ggctggagca acacctgctg
     5461 gggctgaaag tgggcgacaa aaccaccttc tcgctggagc cagatgcggc gtttggcgtg
     5521 ccgtcaccgg acctgattca gtacttctcc cgccgtgaat ttatggatgc aggcgagcca
     5581 gaaattggcg caatcatgct ttttaccgca atggatggca gtgagatgcc tggtgtgatc
     5641 cgcgaaatta acggcgactc cattaccgtt gatttcaacc atccgctggc cgggcagacc
     5701 gttcattttg atattgaagt gctggaaatc gatccggcac tggaggcgta acatgcagat
     5761 cctgttggcc aacccgcgtg gtttttgtgc cggggtagac cgcgctatca gcattgttga
     5821 aaacgcgctg gccatttacg gcgcaccgat atatgtccgt cacgaagtgg tgcataaccg
     5881 ctacgtggtc gatagcttgc gcgagcgtgg ggctatcttt attgagcaga ttagcgaagt
     5941 gccggacggc gcgatcctga ttttctccgc acacggtgtt tctcaggcgg ttcgtaacga
     6001 agcgaaaagc cgcgatttga cggtgtttga tgccacctgt ccgctggtga ccaaagtgca
     6061 tatggaagtt gcccgcgcca gtcgccgtgg cgaagaatct attctcatcg gccacgccgg
     6121 gcacccggaa gtggaaggga cgatggggca gtacagcaac cccgaagggg gaatgtatct
     6181 agtcgaatcg ccagacgatg tgtggaaact gacggtcaaa aacgaagaga agctctcctt
     6241 tatgacccaa accacgctgt cggtggatga cacgtctgat gtgatcgacg cgctgcgtaa
     6301 acgcttcccg aaaattgtcg gtccgcgcaa agatgacatc tgctacgcca cgactaaccg
     6361 tcaggaagcg gtacgcgccc tggcagaaca ggcggaagtt gtgttggtgg tcggttcgaa
     6421 aaactcctcc aactccaacc gtctggcgga gctggcccag cgtatgggca aacacgcgtt
     6481 tttgattgac gatgcgaaag atatccagga agagtgggtg aaagaggtta aatgcgtcgg
     6541 cgtgactgcg ggcgcatcgg ctccggatat tctggtgcag aatgtggtgg cacgtttgca
     6601 gcagttgggt ggtggtgaag ccattccgct ggaaggccgt gaagaaaata ttgttttcga
     6661 agtgccgaaa gagctgcgtg tcgatattcg tgaagtcgat taagtcatta gcagcctaag
     6721 ttatgcgaaa atgccggtct tgttaccggc attttttatg aagaaaacat gcgtttacct
     6781 atcttcctcg atactgaccc cggcattgac gatgccgtcg ccattgccgc cgcgattttt
     6841 gcccccgaac tcgacctgca actgatgacc accgtcgcgg gtaatgtctc ggttgagaaa
     6901 actacccgca atgccctgca actgctgcat ttctggaatg cggagattcc gctcgcccag
     6961 ggagccgctg tgccgctggt acgcgcaccg cgtgatgcag catctgtgca cggcgaatcg
     7021 ggaatggctg gctacgactt tgttgagcac aaccgaaagc cactcgggat accggcgttt
     7081 ctggcgattc gggatgccct gatgcgtgca ccagagcccg ttaccctggt ggccatcggc
     7141 ccgttaacca atattgcgct gttactttta caatgcccgg aatgtaaacc gtatattcgt
     7201 cgtctggtga tcatgggcgg ttctgctggt cgcggcaact gtacaccaaa tgccgagttt
     7261 aatatcgctg ccgatccaga agcggctgcc tgtgtcttcc gtagcggcat cgaaattgtc
     7321 atgtgcggtc tggatgtcac caatcaggca atattaaccc ctgactatct cgctacttta
     7381 ccggagttga atcgcaccgg aaaaatgctc cacgccctgt ttagccacta ccgtagcggc
     7441 agtatgcaaa gcggtctgcg aatgcacgat ctctgcgcca tcgcctggct ggtgcgcccg
     7501 gacttattca ccctcaagcc gtgttttgtc gcggtagaaa ctcaaggcga atttacctca
     7561 ggcacgacgg tggttgatat cgacggttgc ctgggcaaac cagccaatgt acaggtggca
     7621 ttggatctga atgtaaaagg cttccaacag tgggtggctg aggtgctggc tctggtgccg
     7681 taacctgtca catgttatcg gcatgcagtc attcatcgac tcatgccttt cactgatatc
     7741 cctccctgtt tatcattaat ttctaattat cagcgttttt ggctggcggc gtagcgatgc
     7801 gctggttact ctgaaaacgg tctatgcaaa ttaacaaaag agaatagcta tgcatgatgc
     7861 aaacatccgc gttgccatcg cgggagccgg ggggcgtatg ggccgccagt tgattcaggc
     7921 ggcactggca ttagaaggcg tgcagctggg cgcggcgctg gagcgcgaag gatcttcttt
     7981 actgggcagc gacgccggtg agctggctgg tgcagggaaa acaggcgtta ccgtgcaaag
     8041 cagtcttgat gcgataaaag atgattttga tgtgtttatc gattttaccc gtccggaagg
     8101 tacgctgaac catctcgctt tttgtcggca gcatggcaaa gggatggtga tcggcactac
     8161 ggggtttgac gaagccggta aacaggcaat tcgtgacgcc gccgccgata ttgcgattgt
     8221 ctttgccgcc aattttagcg ttggcgttaa cgtcatgctt aagctgctgg agaaagcagc
     8281 caaagtgatg ggtgactaca ccgatatcga aattattgaa gcacatcata gacataaagt
     8341 tgatgcgccg tcaggcaccg cactggcaat gggagaggcg atcgcccacg cccttgataa
     8401 agatctgaaa gattgcgcgg tctacagtcg tgaaggccat accggtgaac gtgtgccagg
     8461 taccattggt tttgctaccg tgcgtgcagg tgacatcgtt ggcgaacata ccgcgatgtt
     8521 tgccgatatt ggcgagcgtc tggagatcac ccataaggcg tccagccgca tgacatttgc
     8581 taacggtgcg gtaagatcgg ctttgtggtt gagtggtaag gaaagtggtc tttttgatat
     8641 gcgagatgtg cttgatctca ataatttgt
//
LOCUS       DAZXUK010000168         8659 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZXUK010000168 DAZXUK010000000
VERSION     DAZXUK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8659
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.168"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(353..559)
                     /locus_tag="ACH3L8_002957"
     CDS             complement(353..559)
                     /locus_tag="ACH3L8_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFT4085985.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            872..961
                     /gene="kdpF"
                     /locus_tag="ACH3L8_002958"
     CDS             872..961
                     /gene="kdpF"
                     /locus_tag="ACH3L8_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFT4085986.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            961..2634
                     /gene="kdpA"
                     /locus_tag="ACH3L8_002959"
     CDS             961..2634
                     /gene="kdpA"
                     /locus_tag="ACH3L8_002959"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFT4085987.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2657..4407
                     /gene="kdpB"
                     /locus_tag="ACH3L8_002960"
                     /pseudo
     CDS             2657..4407
                     /gene="kdpB"
                     /locus_tag="ACH3L8_002960"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4713..5285
                     /gene="kdpC"
                     /locus_tag="ACH3L8_002961"
     CDS             4713..5285
                     /gene="kdpC"
                     /locus_tag="ACH3L8_002961"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFT4085988.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5278..7958
                     /gene="kdpD"
                     /locus_tag="ACH3L8_002962"
                     /pseudo
     CDS             5278..7958
                     /gene="kdpD"
                     /locus_tag="ACH3L8_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            7955..8640
                     /gene="kdpE"
                     /locus_tag="ACH3L8_002963"
                     /pseudo
     CDS             7955..8640
                     /gene="kdpE"
                     /locus_tag="ACH3L8_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
BASE COUNT         1846 a         2235 c         2498 g         2080 t
ORIGIN      
        1 ctgtttttat tgcggtagcg atactaataa caaaattcgg aatttattta gatgctggaa
       61 ttagaaaaag ggatgacggc tttctcacct taccctgaag tttcacgaaa gtgtgtaagg
      121 aagtatgcaa tctaactgga gatttctgat gatctagtcg cttagagcga caatgaaggt
      181 gaccgcatcc tgggtagccc tatgaaagga tactattgaa acgtgatgct aaaacagtga
      241 tgaaatgaat aatacgatgg aaacgtcttc catgttaatt atcatggaag acgttgatgt
      301 tgaacaccta aaccagcccc ctgatacgag gctggcttaa gcgcgcaggc aatcagtaaa
      361 aatcaccggt tgccttttcg gcctgatcca tccacaccgg tttatcgctg gttttcgacc
      421 agacgcggtg caggtagctg taaaagcggg cgcggtcttt ccagaaaagc atgaaaggca
      481 gcgccagaac gcccgctgca accgcatatg tacggcgtaa aaagataagc catgcaggat
      541 attctctgta gagttccata cttttctccc ccttttgtat ctacccggtg aatagcaccg
      601 gaaaaatgaa tttgtttatc tgatgaaaat agtaccgcct tttgtgtaat tttactactc
      661 atccgaccac ttatttttgc ttattgatgg tttatttaca ttcacactgt aattaaatta
      721 cacaaaagtt aaattaatac taaccatcag ttaaatcatg gcctttgcca tttttatact
      781 ttttttacac cccgtccgca gatttttgcg aaatctttgc agccagaatt ctactcttcc
      841 ggtatcactt ttaggccact ggaggtgcac tgtgagtgca ggcgtgatag ccggcgtgtt
      901 gctggtgttt ttattactgg gctatttggt ttatgccctg atcaatgcgg aggcgttctg
      961 atggctgcgc aagggttctt actgattgcc acgtttttac tggtattaat ggtgctggcg
     1021 cgtcctttag gcagcgggct ggcgcggctg attaatgaca ttcctcttcc aggtacagcg
     1081 ggcgttgaac gtatactctt tcgtttacct ggcgtctctg accatgagat gaactggaag
     1141 caatatcttt gtgccattct cggcctgaac atgctggggc tggcggtgct gttttttatg
     1201 ttgctcggtc agcactatct gccgcttaat ccacagcagt tgccggggct gtcgtgggat
     1261 ctggcgctga ataccgccgt cagctttgtc acgaatacca actggcaatc ttatagcggt
     1321 gaaaccacgt tgagctattt cagccagatg gcaggcttaa cggtacaaaa ctttctttct
     1381 gccgccagcg ggattgcggt gatttttgcc ttcatccgtg cgtttactcg ccagagcatg
     1441 agcacactcg ggaatgcctg ggtcgatctg ctacgcatca cgttatgggt gctagtccct
     1501 gtggcgttgt tgatcgcact gttttttatt caacaaggtg cgcagcaaaa ctttctgccc
     1561 tatcaggcag tgaataccgt tgaaggagcg caacagctgt tacccatggg accagtagct
     1621 tctcaggaag cgatcaagat gctcggtact aacggcggtg gcttctttaa tgccaactcg
     1681 tcgcatccgt ttgaaaaccc aaccgcactg accaacttcg tgcagatgct ggcgatcttc
     1741 ttgatcccaa cagcgctgtg ctttgccttt ggtgaagtga cgggcgatcg ccgccagggg
     1801 cgcatgttgc tgtgggcgat gtcagtgatt tttgtcatct gcgtaggcgt ggttatgtgg
     1861 gcagaagttc agggtaatcc tcatctgctg gcgctcggcg cagacagcag catcaatatg
     1921 gaaggtaaag agagccgttt cggcgtgctg gtcagtagcc tgtttgcggt cgtgactacg
     1981 gcggcgtcct gtggcgcggt gattgcgatg catgattcgt ttaccgctct cggcggcatg
     2041 gtgccgatgt ggctaatgca aattggtgaa gtggtgttcg gcggtgtcgg ttctggtctt
     2101 tacggcatga tgctgtttgt cctgctggcg gtgtttattg ccgggctgat gattggtcgt
     2161 acaccggaat atctgggtaa aaaaatagac gtacgcgaga tgaaactgac tgcactggcg
     2221 attctggtta ctccaacact ggtactgatg ggcgcggcgt tggcgatgat gaccgacgcc
     2281 ggacgtagcg ccatgcttaa ccctagcccg catggtttta gcgaagtgct gtacgccgtg
     2341 tcgtccgccg ctaacaacaa cggcagcgcc tttgccggat taagcgccaa ctctccgttc
     2401 tggaactgtt tactggcgtt ctgcatgttt gtcggtcgct tcggggtgat tatcccggtg
     2461 atggcaattg ccggttcgct ggtgagtaaa aagagccaac ccgccagctc cggcacgctg
     2521 ccaacgcacg gcccgctgtt tgttggcctg ttaatcggca ccgtgttgct ggttggcgca
     2581 ctgaccttta tccctgccct ggcgcttggt ccggtggcgg aatatctctc ctgatgatat
     2641 tgagtgagca ctgaatatga gtcgtaaaca actggcgcta ttcgaaccaa cacttgtcgt
     2701 tcaggcgctg aaagaagcgg tgaaaaaatt aaacccgcag gcgcaatggc gcaatccggt
     2761 gatgtttatc gtctggatcg gcagtctgct gaccacctgt attagcatcg cgatggcaag
     2821 cggtgcgatg cctggcaatg cgctgtttag cgcggcaatt agcggctggc tgtgggtcac
     2881 cgtactgttc gctaatttcg ccgaggcgct ggcagaaggc cgcagtaaag cgcaggccaa
     2941 cagtcttaaa ggggtgaaaa aaactgcctt tgcccgcaag ctgcgtgagc cgaaatatgg
     3001 cgctgcggcg gacaaagttc ctgccgatca actgcgtaaa ggtgacatcg tgctggtaga
     3061 agctggcgat attatcccct gcgatggtga agtcattgaa ggcggcgcat cggtcgatga
     3121 aagcgccatc accggggaat cggcaccggt gatccgtgaa tccggcggcg attttgcctc
     3181 cgtcaccggc ggcacgcgta ttctttctga ctggctggtg attgagtgta gcgttaaccc
     3241 cggtgagaca tttctggatc ggatgatcgc gatggtggaa ggcgcacagc gacgcaaaac
     3301 accgaacgag attgccctga ctatcctgct gattgctctg accatcgtct ttttactggc
     3361 aaccgccacg ctgtggccgt tttccgcgtg gggcggtaat gccgtgagtg taacggtact
     3421 ggtggcgctg ctggtctgtc tgatcccaac cactattggt ggcctgttgt cagcgatcgg
     3481 cgtcgccggg atgagtcgga tgctgggcgc gaatgtgatt gccaccagcg gacgtgcagt
     3541 tgaagcggca ggtgacgttg acgttctgct actggataaa accggcacca tcacactcgg
     3601 taaccgtcag gcgtcggagt ttatccccgc gcagggcgtg gatgaaaaaa cgctggctga
     3661 cgccgcacaa ctggcttcgc tggctgatga aacgccggaa ggtcgcagta ttgtgatcct
     3721 cgccaagcaa cgttttaacc tgcgcgagcg cgatgtgcag tcgctccatg ccacctttgt
     3781 accgtttact gcgcaaagcc ggatgagcgg gatcaacatc gacaaccgca tgatccgtaa
     3841 aggttctgtc gatgccattc gccgccatat tgaggctaac ggcggtcact tccctgccga
     3901 tgttgatcaa aaagtcgatc aggttgcgcg tcaaggagcc acgctgctgg tggtggtgga
     3961 aggttctcgt gtgctgggcg ttattgcgct gaaagatatc gtcaaaggcg gtattaaaga
     4021 gcgcttcgcc cagctgcgca aatgggcatt aaaacggtga tgattaccgg cgataaccgt
     4081 ctgactgccg ccgcgatagc tgcggaagcg ggtgtcgatg attttctcgc cgaagcgaca
     4141 ccggaggcca agctggcatt gatccgtcag tatcaggcgg aaggtcgttt ggtagcgatg
     4201 accggcgaca gcaccaacga tgctacggcg ctggcgcagg cagatgtcgc ggtggcgatg
     4261 aactccggca cccaggcggc taaagaggcg ggcaatatgg tggatctcga ctctaacccg
     4321 accaagttga tcgaggtggt gcacattggc aaacagatgc tgatgacccg tggctcgctg
     4381 accaccttca gcattgccaa cgatgtggcg aaatacttcg ccattattcc ggcggcgttc
     4441 gcggcaacat atccgcagtt aaatgcgctg aacatcatgc gcctgcattc gcccgactcc
     4501 gccattctca gtgcggtcat tttcaacgcc ttgattatcg tctttttgat tcctctggcg
     4561 ttaaaaggcg tgagttataa accgcttacc gcttctgcca tgttgcgccg taacttatgg
     4621 atttacggtc tgggtgggct gctggtgccg tttattggta tcaaagtcat tgatttactg
     4681 ctaaccgttt gtagtctggt gtgaggttta ccatgagtgg attacgtccg gcattatcga
     4741 catttctctt tctgttattg attactggcg gcgtttaccc gctgctgacc accgcactgg
     4801 ggcaatggtg gtatccctgg caggccaacg gttcgttgat tcgtgaaggt gatacggtgc
     4861 gcggttcggc attaatcggg caaaatttta ccggcaacgg ctattttcat ggtcgcccgt
     4921 cggcaacagc agaaatgccc tataatccac aggcttctgg cgggagcaat ctggcagtca
     4981 gtaacccgga gctggataaa cagatagccg cacgcgttgc cgcattacgg gccgctaacc
     5041 cggacgccag cacgaacgtt ccggttgaac tggtgacggc gtcggcaagc ggactggaca
     5101 ataacatcac cccgcaagcg gcggcctggc agatcccacg catcgcaaaa gcgcgtaatc
     5161 tcagcgttga acagctcacg cagctgatcg caaaatacag ccaacaaccg ctggtgaaat
     5221 atatcggcca gccggtcgtc aacattgtcg aactcaatct ggcactggat aagcttgatg
     5281 aataacgaac ccttacgtcc cgaccccgac cgtctgctgg aacaaactgc cgcgccgcat
     5341 cgggggaagc tgaaagtttt cttcagtgcc tgtgcaggcg tcgggaagac ctgggcgatg
     5401 ctggcagaag cccagcgact gcgggcgcaa gggctggata ttgtggttgg tgtggtagaa
     5461 acccatgggc ggaaagatac cgccgccatg cttgaagggc tggctgttct gcctctgaaa
     5521 cgccaggcgt accgtgggcg gcatatcagc gagtttgatc tcgatgccgc cctcgcccgc
     5581 cgtccggcgc tgatcttaat ggacgaactg gcgcacagta atgcgccagg ttctcgtcat
     5641 cctaaacgct ggcaggatat cgaagaactg ctggaagctg gcattgatgt tttcacaacc
     5701 gtcaacgttc agcatctgga aagtctgaat gatgtggtca gcggcgtcac cggaattcag
     5761 gtacgggaaa ccgtgcccga tccttttttc gatgccgccg acgacgtggt gctggtggac
     5821 ttgcccccgg acgatctgcg ccagcggctg aaagaaggca aagtctatat tgccgggcag
     5881 gcggagcgcg ccattgaaca ttttttccgc aaaggcaatc tgatcgccct gcgcgaactg
     5941 gcactgcgcc gtactgccga tcgcgttgat gagcaaatgc gcgcctggcg ggggcatcct
     6001 ggcgaagaga aagtctggca cacgcgcgac gcgatccttt tatgcatcgg ccataacacc
     6061 ggcagcgaaa actggtccgc gcggcggcgc ggctgacgtc acggctgggg agcgtctggc
     6121 acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa cgtcgggcaa
     6181 ttctcagcgc cttacgtctg gcgcaggagc tgggcgcgga gacggcaaca ctttctgatc
     6241 cagcggaaga gaaagcggta gtgcgttatg cccgtgaaca taatctcggc aagattattc
     6301 tcggtcgccc agcctcgcgc cgctggtggc gtcgggaaac atttgctgac cgactggcgc
     6361 gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc gcccgcacga
     6421 ttaacaacgc gccggatagc cgctcattta aagacaagtg gcgggtacag attcagggat
     6481 gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag tggctgatgg
     6541 cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg cgtggtggtg gcgctatttt
     6601 atggacgctg gccttcggtg gttgccaccg tcattaatgt ggtgagtttc gatctctttt
     6661 ttatcgcccc acgcggcacg ctcgccgtct ctgatgtgca atatctgctg accttcgcgg
     6721 tgatgttaac cgtcgggctg gtgatcggga accttactgc tggtgtgcgt tatcaggcgc
     6781 gggtagcccg ttaccgcgag caacgcacac ggcacttata tgaaatgtcg aaagctctgg
     6841 cggtgggccg cagtccgcag gacatcgccg ccaccagcga acaatttatt gcctccacgt
     6901 ttcatgcccg cagtcaggtg ttgttgcccg atgacaacgg taaattgcag ccgttaacac
     6961 atccgcaagg aattacgcca tgggacgatg ccatcgcgca gtggagtttt gataaaggcc
     7021 tgcctgcggg cgcgggcacc gacacgttac ccggtgtacc gtaccagatt ttgccgctaa
     7081 aaagcggcga gaaaacctac gggctggtgg tggtggagcc ggggaatctg cgccagttga
     7141 tgatcccgga acagcagcgc ctgctggaga cttttacgct gttagtcgcc aatgcccttg
     7201 agcggctgac gctaaccgcc agcgaagaac aggcgcggat ggcaagcgaa cgtgaacaga
     7261 tccgcaacgc cctgctggca gcgctttcgc atgatttacg cacgccgctt acggtgctgt
     7321 ttggtcaggc agaaatctta acgctcgatc tggcaagcga aggatcaccc cacgcccgcc
     7381 aggccagcga gattcgtcag catgtgctga acactacccg actggtgaat aatctactgg
     7441 atatggcgcg aattcagtcc ggcggcttta atttgaagaa agagtggtta acgctggaag
     7501 aagtagtcgg cagcgcgctg caaatgccgg aaccgggttt attgtcgccc atcaatcttt
     7561 ctctgccaga accgctgacc ttaatccacg ttgacgggcc actctttgaa cgggtgctga
     7621 ttaatctgct ggagaacgcg gtgaaatatg cgggtgcgca ggccgaaatt ggtatcgatg
     7681 cccacgttga gggcgaaaat ctacaactgg atgtctggga taacggcccc ggtcttccgc
     7741 caggccagga gcagacgata tttgataagt ttgctcgcgg gaataaagag tcggcggtgc
     7801 cgggtgtcgg gcttgggttg gcaatttgtc gggcgattgt ggatgtacac ggcggcacca
     7861 ttaccgcgtt caaccgaccg gaaggtggtg cctgttttcg tgttacactt ccccagcaaa
     7921 ccgctcctga acttgaagac tttcatgagg atatgtgaca aacgttctga ttgttgaaga
     7981 tgaacaggct attcgtcgct ttctgcgcac ggcgctggag ggcgacggga tgcgcgtctt
     8041 tgaggccgaa actctgcaac gcggcttgct ggaagcggca acccgtaagc cagatttgat
     8101 tattctcgat ctcggcctgc ccgatggtga tgggattgag tttatccgcg acctgcgcca
     8161 gtggagcccg gtgccggtga ttgtgctttc cgcacgcagc gaagagagcg acaagatcgc
     8221 cgcgctggat gccggagcgg atgattatct gagtaagccg tttggtattg gcgaattgca
     8281 ggcccgtctg cgcgtcgctt tacgccgcca ctccgccact ccgccaccgc cgcgcccgat
     8341 ccgctggtga aattttccgg tgttaccgtc gatttagccg cccgcgtgat tcaccggggt
     8401 gaggaagagg tgcatctcac accaattgag ttccgcctgc tggcggtgct gctcaacaat
     8461 gccggaaaag tactcaccca gcgccagctc cttaaccagg tgtgggggcc aaacgcggtc
     8521 gaacacagtc actatttgcg tatttatatg ggacatctgc gacaaaaact ggaacaggat
     8581 ccggcccggc ctcgccactt cattactgaa accggtattg ggtatcggtt tatgctttga
     8641 attgttattc aataaattc
//
LOCUS       DAZXUK010000169         8651 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZXUK010000169 DAZXUK010000000
VERSION     DAZXUK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8651
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.169"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..558)
                     /gene="aroC"
                     /locus_tag="ACH3L8_002964"
     CDS             complement(<1..558)
                     /gene="aroC"
                     /locus_tag="ACH3L8_002964"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFT4085989.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKI"
     gene            complement(593..1525)
                     /gene="prmB"
                     /locus_tag="ACH3L8_002965"
     CDS             complement(593..1525)
                     /gene="prmB"
                     /locus_tag="ACH3L8_002965"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFT4085990.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            1691..2242
                     /gene="smrB"
                     /locus_tag="ACH3L8_002966"
     CDS             1691..2242
                     /gene="smrB"
                     /locus_tag="ACH3L8_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFT4085991.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(2346..3236)
                     /locus_tag="ACH3L8_002967"
     CDS             complement(2346..3236)
                     /locus_tag="ACH3L8_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFT4085992.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(3223..3747)
                     /locus_tag="ACH3L8_002968"
     CDS             complement(3223..3747)
                     /locus_tag="ACH3L8_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4085993.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(3744..4214)
                     /locus_tag="ACH3L8_002969"
     CDS             complement(3744..4214)
                     /locus_tag="ACH3L8_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4085994.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(4211..4759)
                     /locus_tag="ACH3L8_002970"
     CDS             complement(4211..4759)
                     /locus_tag="ACH3L8_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4085995.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(4764..5486)
                     /locus_tag="ACH3L8_002971"
                     /pseudo
     CDS             complement(4764..5486)
                     /locus_tag="ACH3L8_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(5506..8146)
                     /gene="yfcU"
                     /locus_tag="ACH3L8_002972"
                     /pseudo
     CDS             complement(5506..8146)
                     /gene="yfcU"
                     /locus_tag="ACH3L8_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(8228..>8651)
                     /locus_tag="ACH3L8_002973"
     CDS             complement(8228..>8651)
                     /locus_tag="ACH3L8_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4085996.1"
                     /translation="DDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMT
                     TTFTGTVSSANSGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTST
                     NKGKAKQTLNFNAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT         2173 a         2183 c         2069 g         2226 t
ORIGIN      
        1 gattttgtcg gggtccgggc agaagaacgg attttgttca accagcgacc agtctttgat
       61 ttccagcgga atgtcgccca tctgggtcag gcagccgcgg atttcaatac caaatttctc
      121 ggcgagatat tttttggcaa tcgcccctgc cgccacgcgc atggcggttt cgcgggcgga
      181 agaacgtcca ccgccgcgat aatcgcgcag accgtatttt tgttcgtagg tgtaatcagc
      241 atggcctgga cggaagacgt ctttaattgc gctgtaatcc tgagaacgct ggtcggtatt
      301 ttcgatcaac aagccaatgc tggtgccagt agtaacgcct tcaaaaacac cggagagtat
      361 tttgacctga tccggctcgc ggcgctgggt ggtatagcgc gatgtcccag ggcgacgacg
      421 atcaaggtca tgttgcaggt ccgcttccgt cagcggaatg cctggcggaa caccatcgac
      481 gatgcagccg agcgccagcc cgtgcgattc gccgaaggtg gttacgcgaa agagttgtcc
      541 aattgtgttt ccagccatca cggctccgtt attgttgtgt ttgcgtgttt acttaatctt
      601 tataaatcgc gaaatgttct cgtgcggcaa taagctgctc tttagtgagc ataaacacac
      661 catcgccgcc gttatcaaac tccagccagg tgaacggaac atcgggatat tgttccataa
      721 gatgtaccat gctgttgccc acttcacaaa tcaacacgcc atcatcagca aggtaatctg
      781 ccgcgttacc gagaatgcga cgcgtcagtt tcaggccgtc ggtgccagat gccaggccca
      841 gttccggctc gtgacggtat tcgtttggca ggtcggacat atcttccgcg tcgacatacg
      901 gcgggttagt gacaatcaga tcgtattgca ctttcggcaa atcacggaac agatcggaac
      961 gaatcggaat gacgttgtgg atcagaccgt gttcttcgat gttctgttcg gcaaccgcca
     1021 gcgcgtcggg agagatatct accgcgtcga cttccgcttc cgggaaggca taagcacagg
     1081 caatggcgat gcagccgcta ccggtacaca tatctaaaat atgctgcggt tgcttgctga
     1141 tgagtccggc aaatttattg ttgatcagtt caccaatcgg cgagcgcggc accagcacgc
     1201 gttcatcgac gtaaaattca tgaccgcaga accaggcttt gttggtcagg taagccaccg
     1261 gaatgcgctc gttgacgcgg cggatcacgc gttcaacaat acggtgtttt tcgctggagg
     1321 tcagacgcgc ggtgcgcata tcttccggga tatccagcgg caggtagagc gatggtaaca
     1381 ccagctgtac ggcttcatcc cacgggttat cggtgccgtg accgtaccag atatttgccg
     1441 cgctgaagcg gcttaccgac cagcgcaata tgtcctgaat ggtttgcagc tcatttactg
     1501 cttcatcaac gaaaatttta tccacgtatt cctccagggc atgatcgcaa taatttcggc
     1561 ggctagtttg ccatgaagat gacgataaat cagcattcac gcgcggtgag tgaggaaaaa
     1621 tacgtttaaa acgatcgatt gtgctacgag tcgggtaaac tgtaggaaaa ttagaaatag
     1681 agacagataa atgaaaaaga aaacaacact tagcgaggag gaccaggctc tgtttcgcca
     1741 gttgatggcg gggactcgca agattaagca ggacacgatt gtccaccgac cgcagcgtaa
     1801 aaaaatcagc gaagtgccgg tgaaacgctt gatccaggag caggctgatg ccagccatta
     1861 tttctccgat gagtttcagc cgttattaaa taccgaaggt ccggtgaaat atgttcgccc
     1921 ggatgtcagc cattttgagg cgaagaaact gcgccgcggc gattattcac cggaattgtt
     1981 tttggattta cacggtctga cgcagctgca ggccaagcag gaactgggcg cgttgattgc
     2041 cgcctgtcgc cgcgaacatg tgttttgtgc ctgcgtgatg catgggcacg ggaagcatat
     2101 tttgaagcaa caaacaccgc tgtggctggc acaacatccg catgtgatgg catttcacca
     2161 ggcaccgaaa gagtacggcg gtgatgcggc tttgttggtg ttgattgaag tggaagagtg
     2221 gttgccgccg gagttgccct gaatgctggc agcaaatgcc ggagcagcgt aaacgcctta
     2281 tccggcctac aaatatgtga taattcaata aattgcaatt cacttcgtag gcctgataag
     2341 acgcatcaga gcaatactac gaacttcagt tcattcaata catactgaaa acgacacgtc
     2401 gaatcaatcg gttgcccact actggtattc acattcacca caatatcgtg tagtacttta
     2461 tccgttaccg gatgacgagt tggcgtaacc gttaccgttg atttatcaca accgtaagag
     2521 gtaaaggtta acaattctga taccgcgcgg cagtatgctc ctctgccatc attatcagaa
     2581 aaattactgc catcacagag tctgccgcct tccatcaggt aggtcgaaag cgtggtggtc
     2641 actgttgacc ccgtggactg aattcgtaac gccttattat ggctgacata attttcactc
     2701 caggagatat cgtatgaagc gaccggtatc gttgggcata ttgtgttatg cactgtaggg
     2761 gccgtatagc ttccaaatcc gggtaaatct gtataggaat aaaaggatgc gatccaaggg
     2821 caaccataat catcagatgc aacggtaatt ttaaacgtca tatatgcaca acgaaaaatt
     2881 ttcgtatcta tgccagggca atttgagtta ttaattgtat caaagtcagt cagtaccaaa
     2941 gttggagctg aacccgaccc agaccaggaa ataacccgca acgtatcgcc tttatacgca
     3001 aaagaatcat atttcccata tgcaacctcc tttggcgtta tcaccgtaac attgaaaaca
     3061 ctgctcgcta cagcaccggt taccttgtcc atggcataat aaactctcac actgtcaaaa
     3121 ctcgcactca taagtttatc tccagcataa ctcttagatg cgccgacaac actcagaacg
     3181 agcagcatta ggctcgcgaa aattatggtc cattttttca tttcagaaat actcaaaggt
     3241 tgccgtcact gtggcactaa agtctccgga cgttacatct cgcccgctct ttgcctgtaa
     3301 ccaggtatta aaatttaggc tgttatttcc ctgattcagc gggagcgtga cgcctgttgt
     3361 attgttgatt ggcaccgatg tgcctgccgc agtttcaata ccaatcccag cgccggatgc
     3421 tgatgacgag gagtcaaagg ccagaaagcc gggtagagca ctatcttctg ttcctgtcaa
     3481 cgttactttg acattaaaca atgtagaact atgacaatct ttcaattgaa acaccaatgg
     3541 cactcgctct gactgcccaa ggttcattaa atcactggcg gcaatggcag gaaaatgtac
     3601 ttctgccaac tggctaccat taatcactgg cgtacacgat ttactgatta aattgccggt
     3661 aaagtgcaaa ttatctcctg ctattgtggt tccactgccc agcaacatca ggctactaat
     3721 aaaaaacaac gtacgctttt tcttcattgg taatctacct gtaacgtggc ccaggcctca
     3781 aagtccgcct ctggcagaat gacaccactc ttttttactg gcacagccgt taacaccggt
     3841 aaatcggttt cattcaccac aagtggcgta ttgatcgtaa aactctgccc tgcctgtttg
     3901 agctgaatgc ctaaaccagt gatattgctg cttactgcag cattatcaaa atcgcttggt
     3961 gtgccactcc agctcaaggt catttccatt aatgggtcgc gaaccgccgt atcgcaggta
     4021 atggaatacg gcacggtttg ctcataattg ctgccattaa tgtcatcaat aagcacatcc
     4081 tgaaaagtta tttcaatggt ttgagcgttg ctaatcgtgc agttaggcgg ctgcactaaa
     4141 taaccgtgaa aagagacgtt tgagagtgcc atcgagcaaa agccccacag caaaataagc
     4201 gagatccgtt tcattggtaa tccaccacaa ttgtggcact ggcgttaaat tcagctgccg
     4261 ctaacgttgt accactctgt ttcaccggca cagcttccag aacgggaact cccgaggaaa
     4321 aattaaatgg caaccatgag ccagaggtta agtcgagtat tgtgtgatca ctacttttct
     4381 gaacccgaat accaaaccca ggaatacccg tgctgataac tgtttcaccg ttaatcacga
     4441 ccgttgtcgc ctgcatctgt agacgcaggt catccattgc cagattgtta caaacaagcg
     4501 agtagtcaat gggacgttta taatcaaccc tattgatctt attaatgaat acattaccaa
     4561 actcaactgc cgcaccattt accgtacaag gaggaggggc atcgacaagc acccgtaaat
     4621 tcatacttac tgacttactc gctgcaactg caaagccgct accaacacaa catatcagta
     4681 acccagttac cgcccagaca gaacaagatc ctttcatact ggctctcccg gcctcagcct
     4741 ttgcggctct gctcttcatc gacctagcaa ctgttaccgt tgcaagtgaa aaagagcggc
     4801 atccgagcgc catagtcgtt gacataggtc agtaccggca cgctacccat cttcacattc
     4861 agcggtgcag aggttttcgg cgacatcact attggcgaga aaccggctgc cggattgcca
     4921 tttttctgtg tgctggcatt actgaaaatg acgtaatacg acgtcgggtt attgacggta
     4981 aattcctgac cgttgcgggt taaagtaatc ttatattgct aggggttttt catgctgacg
     5041 ttctccagcg ccttcggtcg ccagaacaac ttaatacgcg tttgtagtgc tatttgcagc
     5101 gtgttggctt tgtttgattt tggcgggatc tcacgcacgt tgaagtagaa cagggtttca
     5161 cgatcggcag gcaatttgtt gatatccggc attccctgca cttttacctg cccgttcatc
     5221 attgaatcga tgcgttgcac cggtggcaga acagttaacg gtgaactgat cttgttaccc
     5281 ttttcatctt cgatccagct ttgggccaga taaggcagtt tcggatcgtt attgcgcaac
     5341 gtcacgctga ttgatttgtc gctttcatta aatatcaaac gggtacgatc tggcgttaca
     5401 gaagcctggg cagacaaact ggcagcgcta agcagtaacg ctagcgtcgt agcacccaat
     5461 ttggcagagc aaagtaaatc agacatggat cattccttaa tcacgttatt ggttagctga
     5521 aacagaaaca gggggattgg tgggtgtcac ctgttgcgtc tgctcctgaa tgacaggctt
     5581 tatgtcatca ggaacaaccg gcgcgacgtt gcctttatgc tggcatggca gtaacaagcc
     5641 gttaaacaga tcggcaggca gcgggtccgg caggttgata tcgcaatgcg caacaccact
     5701 ccagaacaca ctcatgtgtt cgccaggttt cactccagcc agataaacat tgccgtcatc
     5761 atcgacaagg cccactgtct gctcgttatc attttttact tctgcgccaa acggaggatg
     5821 gctgccgtct tgtaaacgca gcaccgccat cgctttttga ccactgatga cggcaaattt
     5881 gcggtagcca atcgcccctt cagttagcgt ggcttgtacc accgactggg ttgcttcggc
     5941 gttttccggc agtttgttga ggtcgatata cgcctgattg cggtaatagt tattgacgtc
     6001 agaaacgacg gctttaccaa acatattggt ataaacagca gcaccgttac cttctaccgg
     6061 aacatcggca acgccatcgg catcaatcaa caagcgtgtc ccgcccatat tctgggtacg
     6121 gtgaagtgcg ccaccgtggg cagtaagcgt tgcgccgccc tgtaacgaca agcccgcaga
     6181 ggtgtattgc ccttcatggt agttcgcact gaggtcaacc tgcgccagcg aaccatcatg
     6241 gctgtaatag ccgtcaacgc tggtgtgttt gtcactggtg ccgacgttca actgatagtg
     6301 agtcgcgtca tcgacacggc tgaaataacc gacctgactg ctgtccgtcc cactgccata
     6361 gtaccgttat agctaacggt actgttgtcg ccccacggca tactgagcga aatgtacatg
     6421 cctttgtcgg cctggttgtc atactcgtaa cggtagccag tcatcgagat gctgacatta
     6481 cgaatactgc ccatattgaa atagtgcgag agcatgatgt tgtagtttgt ctgctcctcg
     6541 cgatcccagt aggtatggcg ggtatagttg agataaaccg aaacacccgc atcgcggaag
     6601 ttctggttat aagtggcggt gtacatctct ttgtcgttgc ccgtgcgtac cattccgctg
     6661 tcactggcat ccagatactc gctcatggtc ataaagttct cttccgagaa gcgatatcca
     6721 gcaaaagtaa cgcggctgtt gagctggtca aagtctttgg aataactcac acggaaggag
     6781 ttaccgtcca gcgaaccttt gccataagcg gtgtctttat ccagtttggt gtgcgagtga
     6841 gtaacatcaa acgcgaccgc gccgaatgta gacaaatcgc gaccgacacc aagcgccgca
     6901 gactgatagt tttcatctcc cagtgcgccg ccatataacg accagccgtt agcgatcccc
     6961 caggaagctt cagcaccaga aaaaaattca ccctcgacat ggtgtcccca ctcttgcgga
     7021 cggcccatca tgatcttata gcgaacctga cctggacgag tgaggtatgg catcgaggcg
     7081 gtgctgatgt catattcctg cacctggccg ttctgttctt caatgcgaat atgcaacgta
     7141 ccggagacag aatcaccaag atcctgaata cgaaacggtc cagccggaac ctgcgtttcg
     7201 taaatcacac gccccatctg gctgacggtc acttttgctg tggtgtgtgc cacgccggaa
     7261 atgtctggcg cgtagccgcg cagattggga ggcaacattt gatcgtcagt actgacactg
     7321 ccaccaacat agttaaaacc atcaaaaata tcggatctga ggtaatcctc gcccagcgcc
     7381 agtttggctt ttaatgatgg taacgcccgc ctggcatagt agcgactcca ctcccatttt
     7441 tttgagtttc atcgccgctg aattcatcgt catcattact gcgagtatgt tgatagttag
     7501 tctgccagtc agcacgcata cgccacggcc ctaggttaac cccaaccgtc ccgttgccgc
     7561 tgatctcgtt actatcatca ccgccatttt cttcgtgccg ggtttgtgcg ttgatgctgt
     7621 agtccgcgac gatcccggag atgccgtcat cccaacgtga aggcggatcc ctgtcgggat
     7681 aagtatattc gaggtaagcc tgtggcagtg aaatgactaa tgcggactgg cttaaatcag
     7741 ccttaatttc catgccttcc agttgaccgg atttcaggca tttagcatcg tggctccatt
     7801 gcagattttt cgccacgtct tcttttaaac caaactgcgc taccagttcc ggtgtcagac
     7861 aagcatagct tttgctcgcg tcatcttcac cagcatacca gtaaatatcg tactcttccg
     7921 ccaatggctg tttatttagt tgaacctgta aattgtattt tccgggctca acatatccct
     7981 ggctggaaaa acgcttcata tcaatttttg tgtcgccttt taattccaga aaacgggaat
     8041 caaactgaat gtcgtcttca gcccagacac tgctataact ccctgacatt gccagcgcaa
     8101 tgcaccacgg aagaatccgt aatcgaaaaa gagaatggtc aggcatacac gtttctatcc
     8161 gtttttattc ttgttcggtc aaaatacatt taaatccccc atagcagtgt cgctatgggg
     8221 gtatgcctta caggtaagta atctggaagg tggtatttgc ttcaaaatta cccagatctg
     8281 gcgcatcagc tgcgccgacc agccaggcgt taaagttcag tgtctgcttc gctttgcctt
     8341 tgttggtaga agtgtctttc actattttct ggtcaatacc catgccgctt ttgtaagagg
     8401 tacccagaga gtcaccgatc gccaggctaa cattgttaaa tgccgcaccg gtatcggtat
     8461 tgaaaatggt gtaataattg ccgctatttg cagaagaaac ggtaccagta aaggtagtgg
     8521 tcattgtttc ctgagtatca aatacgcaat cttgcagacg aatctggaaa cttttcggcg
     8581 tggtggtgcc gttatttttc agtttttcgg caccgatatc acccatatca acttccagtt
     8641 tgtgatcgtc c
//
LOCUS       DAZXUK010000170         8611 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZXUK010000170 DAZXUK010000000
VERSION     DAZXUK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8611
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.170"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(70..810)
                     /gene="cysQ"
                     /locus_tag="ACH3L8_002974"
     CDS             complement(70..810)
                     /gene="cysQ"
                     /locus_tag="ACH3L8_002974"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFT4085997.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            1000..2943
                     /gene="cpdB"
                     /locus_tag="ACH3L8_002975"
     CDS             1000..2943
                     /gene="cpdB"
                     /locus_tag="ACH3L8_002975"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFT4085998.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3069..3449)
                     /locus_tag="ACH3L8_002976"
     CDS             complement(3069..3449)
                     /locus_tag="ACH3L8_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFT4085999.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3538..4398
                     /gene="qorB"
                     /locus_tag="ACH3L8_002977"
     CDS             3538..4398
                     /gene="qorB"
                     /locus_tag="ACH3L8_002977"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFT4086000.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            4507..5472
                     /locus_tag="ACH3L8_002978"
     CDS             4507..5472
                     /locus_tag="ACH3L8_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFT4086001.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5580..6242
                     /gene="ytfE"
                     /locus_tag="ACH3L8_002979"
     CDS             5580..6242
                     /gene="ytfE"
                     /locus_tag="ACH3L8_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFT4086002.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6287..7699)
                     /gene="cycA"
                     /locus_tag="ACH3L8_002980"
                     /gene_synonym="dagA"
     CDS             complement(6287..7699)
                     /gene="cycA"
                     /locus_tag="ACH3L8_002980"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFT4086003.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(8007..>8611)
                     /gene="fklB"
                     /locus_tag="ACH3L8_002981"
     CDS             complement(8007..>8611)
                     /gene="fklB"
                     /locus_tag="ACH3L8_002981"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFT4086004.1"
                     /translation="DTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIADALEGKH
                     PAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTESGLQFRV
                     INQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIEALTLMP
                     VGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         2173 a         2276 c         2187 g         1975 t
ORIGIN      
        1 tataactttt taggttggga taaatcagga tggcagaatc aggaaataca ctgtttctgc
       61 catctgaatt tagtaaatgg acactctgaa ccccggattc aggaacgact cgcgcggagt
      121 gtaatccagc ggtttaccct gccagtcgtg aacgtgcgct ccggcagctg cagccacagc
      181 atgtccggcg gcggtgtccc aaatattcgt tggtccgaag cgcgggtaca gctgcgcctg
      241 tccttccgcc accaggcaga atttcagcga agagccgatg gacgtggtct gatgttcgcc
      301 aagctgttgc agatactctt tcagctccgc atctgcatgg gaacggctga taaccaccag
      361 cggcgggcgc gcatcgcgga cctgaatcag cttgcgcaca ccgcactctt ctttccaggc
      421 cttgccttct gccgcgctgt acattacgtt cattaccggc gcatacacca cacctaaaat
      481 cggtttgcca tggtcaatga gcgcaatgtt aacggtgaat tcgccattac gtttaataaa
      541 ctctttagta ccatccagcg ggtctaccag ccagtaacgc tgccagtgct gacggacttc
      601 ccaaccggga ggatcttctt cagaaaggac cggaatatcc ggtgccagcg tacgtaaacc
      661 gtccatgata acggtgtgag cggcaatatc cgctgccgtt accggagaat tgtccgcttt
      721 gctgacgacg tccatcggtt tcgtcccgtc gtagacctgc ataatggcat cgcctgcatt
      781 ccgtgcaagc tggcatactt gatctaacat ttctccacct cgtctctgtg agcggtgtta
      841 acttattgtt ttacttatac cctatcgtta atgaatgcgc caactgtgat agtgtcatca
      901 ttttcaaagc gtaaaattgt ggcattcttc actgttctat aagtaaggcg tttattctcc
      961 cttttctttc gtattcccga tgataaaagg atgtccctga tgattaagtt tagcgcaacg
     1021 ctcctggcca cgctgattgc cgccagtgtg aatgcagcga cggtcgatct gcgtatcatg
     1081 gaaaccactg atctgcatag caacatgatg gatttcgatt attacaaaga caccgccacg
     1141 gaaaaattcg gactggtacg tacggcaagc ctgattaacg atgcccgcaa tgaagtgaaa
     1201 aacagcgtac tggtcgataa cggcgatttg attcagggga gtccgctggc cgattacatg
     1261 tcggcgaaag gattaaaagc aggtgatgtt catccggttt ataaggcgct gaatacgctg
     1321 gattatacgg tcggtacact cggcaatcat gaatttaact acggtctgga ttacctgaaa
     1381 aatgcgctgg cgggagcgaa attcccttat gtaaatgcca acgtcattga cgccagaacc
     1441 aaacagccaa tgtttacacc gtatttaatt aaagataccg aagtggtcga taaagacgga
     1501 aaaaaacaga cgctgaagat tggctatatt ggcgtcgtgc cgccgcaaat catgggctgg
     1561 gataaagcta atttatccgg aaaagtgacg gtgaatgata ttaccgaaac cgtgcgcaaa
     1621 tacgtgcctg aaatgcgcga gaaaggtgcc gatgtcattg tcgttctggc gcattccggg
     1681 ctgtctgccg atccgtataa agtaatggcg gaaaactcag tttattacct cagtgaaatt
     1741 ccgggcgtta acgccattat gtttggccat gctcacgccg ttttcccggg taaagatttt
     1801 gctgatatcg aaggggctga tatcaccaaa ggcacgctga atggtgttcc ggcggtaatg
     1861 ccgggcatgt ggggcgatca tcttggggtg gtcgacttac aactcagtaa tgacagcggt
     1921 aaatggcagg tgacgcaggc gaaagcggaa gcacggccga tttacgacat cgccaataaa
     1981 aaatccctcg cggcggaaga cagcaagctg gtagaaacac tcaaagccga tcacgatgcc
     2041 acacgccagt tcgtcagcaa gccaatcggt aaatctgccg acaatatgta tagctatctg
     2101 gcgctggtgc aggacgatcc gaccgtgcaa gtcgtgaaca acgcgcaaaa agcgtatgtc
     2161 gaacattaca ttcagggcga tccggatctg gcaaaactgc cggtgctttc agctgccgca
     2221 ccgtttaaag ttggtggtcg caaaaatgac ccggcaagct atgtggaggt ggaaaaaggc
     2281 cagttgacct tccgtaatgc cgccgatctt tatctctacc ccaatacgct gattgtagtg
     2341 aaagccagcg gtaaagaggt gaaagagtgg ctggagtgct ccgccggaca gtttaaccag
     2401 attgctccta acagcacgaa accacagtca ctcatcaact gggatggttt ccgcacttat
     2461 aactttgatg ttattgatgg tgtgaattat cagattgatg ttacccaacc cgcccgctat
     2521 gacggcgagt gccagatgat taatgccaat gcagagcgga ttaagaacct gacctttaat
     2581 ggcaagccga ttgatccgaa cgctatgttc ctggttgcca ccaataacta tcgcgcttac
     2641 ggcggcaaat ttgccggtac gggcgacagc catatcgctt ttgcttcacc ggatgagaac
     2701 cgctcggtgc tggcagcgtg gattgctgat gagtcgaaac gtgcggggga aattcatccg
     2761 gcggcagata acaactggcg tttagcaccg atagctgccg ataagaaact ggatatccgt
     2821 ttcgaaactt ccccgtcaga taaagccgca gcgtttatta aagagaaagg gcagtatccg
     2881 atgaataaag tcgcgaccga tgatatcggg tttgcgattt atcaggtgga tttgagtaag
     2941 taaaacactt ctttttcggc ctataaatca accgcatccg gcatttattg gcggatgcga
     3001 tgctgccgta tcttatccac cctacaagcc atgaaccgta gaccggataa gcgcagcgca
     3061 tccggcagtt atgccgcacg ttcatcccgc accgccaaca cctcgggcaa attcaactca
     3121 atccagtccg ccagtgcagc aaccttttcg ctcacctgct cgcccagcgg cgtgaggcta
     3181 tattccacat gcggcggcac caccggatac gcgatacggt taagaaaacc atcctgttct
     3241 aacgcctgta acgactgcgc aagcatcttt tcactcaccc caccgatttt gcgccgcagg
     3301 tcgctaaagc gatgagtacc ttcgcgtagc gccaccagaa tcaacacccc ccaacggctg
     3361 gtgacgtgtt tcaacacctc gcgcgacggg cactgttccg caaagagatt accctctttc
     3421 agttgttgtg acaggctaac ctgactcatt tcatacttac ctttttgcac gtacttacta
     3481 aaagtaagtt taggtgttag cgtatttaaa cacaagacaa aacgatggag acttcccatg
     3541 atcgctatta ctggtgccac tggccaactt ggtcactata ttattgaatc cttgatgaaa
     3601 acggttcctg ccagccaaat agtggctatc gttcgtaatc cggcaaaagc ccaggccctg
     3661 gcagcacaag gcattaccgt gcgtcaggct gactacagcg atgaagccgc actgacatct
     3721 gcacttcagg gagtggaaaa actactgctg atctcttcca gcgaagtggg tcaacgtgcc
     3781 ccgcagcatc gtaatgttat taatgccgca aaggcggctg gcgtgaaatt tatcgcttat
     3841 accagcctgc tacatgcaga tacctccccg ctcggcctcg ccgatgagca catcgagacg
     3901 gagaaaatgc tggctgattc tggcatcgtt tacaccctgc tgcgcaacgg ctggtacagc
     3961 gaaaactacc tcgccagcgc cccggcagca ctggaacacg gcgtatttat cggtgcggct
     4021 ggcgatggca aaatcgcctc agcaacgcgg gcagattatg cgacagctgc ggcacgcgtg
     4081 attagcgaag ccggtcacga aggcaaggtt tacgaactgg cgggcgatag tgcctggacg
     4141 ttgacacagt tagcggcaga gctgaccaaa cagagcggca aacaggttac ctatcaaaat
     4201 ctgagcgaag ccgatttcgc cgcggcactg aaaagcgtcg gactgcccga cggactggcg
     4261 gatatgctgg cggattctga cgttggcgca tcgaaaggtg gtctgtttga taacagcaaa
     4321 acgcttagca aattgattgg ctgcccaacg acaacgttag ccgaaagcgt aagccatctt
     4381 tttaatgtta ataactagtt aattaaagtg gcatcctccc gcatcctctc tgataatgac
     4441 gggatgccgg gagcaatcat gtctgcttcc tgaacgtttc ttctgacaga ccaatggatg
     4501 ccagtaatga ttagcggcgt gctgtacgcc ctgttagcag ggttgatgtg ggggcttatt
     4561 tttgtcgggc cgttgatcgt gccggaatac ccggcgatgt tgcagtcgat ggggcgttat
     4621 ctggcgttag ggttaattgc gctacccatt gcctggctgg gacgcgtgcg tctgcgtcag
     4681 ttggcgcgtc gggactggct taccgccttg atgctcacta tgatgggcaa cctcatttat
     4741 tacttctgcc ttgccagtgc cattcaacgt actggcgcgc ctgtttccac gatgattatc
     4801 ggcaccctgc cggtggtgat tcctgtcttt gccaatctgc tttatagcca gcgcgacggc
     4861 aaactcgcgt ggggaaaact cgccccggca ctgatttgta ttggcatcgg cctggcgagt
     4921 gtgaatattg ctgagttaaa ccacggactc cccgattttg actgggcacg ttatacctct
     4981 ggcatcgtgc tagcgttagt ttccgtggtc tgctgggcat ggtatgccct gcgcaacgcc
     5041 cgctggctgc gggaaaatcc cgacaaacat ccgatgatgt gggcgacggc gcaggcgctg
     5101 gtcacgctgc cggtttctct catcggctat ctcgtcgcct gttactggct gaatatacaa
     5161 acgccggact tctccttacc ttttggcccc cgtccgctgg tgtttattag tctgatggtt
     5221 gcgatagccg tgctttgctc atgggttggc gcactctgct ggaacgtcgc cagccagcga
     5281 ttaccgacag tgattctcgg gccgctgatt gttttcgaaa cgctggcagg tttgctgtac
     5341 acctttttac tccgccagca aatgccgccg ctaatgacgc tgagcggtat cgcgctgtta
     5401 gtgattggcg tggtcatcgc ggtcagagca aaaccagaaa aacctttaac tgaatctgtc
     5461 tcagaaagtt gacatgctgg cagtgagtta aataagcctc tgctacgtaa gggttatagc
     5521 ttttgcctta aaggtgtatt taaaatacat cttatcttat taagaatgag gtatcagcta
     5581 tggcttatcg cgaccaacct ttaggtgaac tggcgctctc tatccctcgc gcttcggctc
     5641 tgtttcgtaa atatgatatg gattactgct gtggcggtaa gcagacgctg gcgcgcgcgg
     5701 cggcacgtaa agaactggat gttgaggtca ttgaagctga actggcaaag cttgctgaac
     5761 aaccgattga gaaagactgg cgtagcgccc cgctggcaga aatcattgac catatcatcg
     5821 tgcgctacca cgatcgtcac cgcgagcagc tgccggagct gatcctgcaa gcgactaaag
     5881 tcgagcgcgt tcacgccgac aaaccgagcg tgccaaaagg gctgacaaaa tacctgacca
     5941 tgctgcatga agagctttcc agccacatga tgaaagaaga gcagatcctc ttcccgatga
     6001 tcaaacaagg catgggcagc caggcaatgg ggccaatcag cgtaatggaa agcgagcacg
     6061 atgaagcggg cgaactgctg gaagtgatca aacacactac caataacgtc acaccgccgc
     6121 cagaagcctg caccacctgg aaagcgatgt ataacggcat taatgaactg attgatgacc
     6181 tgatggatca catcagtctg gaaaacaatg tactgttccc acgcgcccta gcgggtgagt
     6241 gatctaaaag ctggatggca ttgcgccatc cagcatgata atgcggttat ttccgcagtt
     6301 cagcagcccg cttcttacca ataaacagcc agcccaaccc cagcgcgata aaccacagcg
     6361 gggtaaccag cagcgcctgg cgagtgtcat cttccagtgt cagcaacacg accacgaaca
     6421 caaagaacgc catacatacc cagcacatca gcttgccgag cggcatcttg tagatcgact
     6481 tttcatgcag atgaggacgc tgtttgcggt acaccaggta agagcaaagg ataatcgtcc
     6541 agacgaacat aaacagaatc gcggaaacgg ttgtaatcat cgtgaacgcg ccaatcacgc
     6601 taggattcac atacaacatc accacgccgc cgagcagaca gatacacgag aaggtcaacc
     6661 ctttcgcggg tactgcgcgc ttagaaagtt tagcgaacgc tttcggcgca acaccttcct
     6721 gcgccagacc aaacagcata cggctggtag agaagacacc gctgttagcg gaagacgccg
     6781 cagaggtcag caccacaaag ttgatcacgc tggcagccgc aggcaaacct accaacacaa
     6841 acagctcaac gaacgggctt ttctccggga ctaccgaact ccacggtgtc acggacataa
     6901 tcacaatcag cgcgaagacg tagaacataa tgatacggat cggaatggag ttaatcgcgc
     6961 gtggcagtga tttctccgga tctttggttt ccgcagctgt tgtacctacc agctcaatcc
     7021 ccacgaaagc gaaaaccgct atctggaatc cggcaaagaa gccacttaaa cctttcggga
     7081 accagccgcc gtcattccac aaatgcgcga atgacgcttc cacaccggtc ggtgactgaa
     7141 agtgcatcgc caccatgacc aggccgacga caatcagcga cacgatggcg acgattttga
     7201 tcatcgcaaa ccagaactcc atctcaccga acattttcac ggtggcaagg ttgagcgtca
     7261 gcagcagcac tatcaccgcc agcgaggcga cccagtcgga gagatcgggg aaccagaact
     7321 gagcataagc cgtgatcgcc accacgtctg ccataccggt tacaacccag cagaaccagt
     7381 aagtccagcc ggtgaaatat cccgcccacg gccccagtaa atcggaagcg aagtcactaa
     7441 aagatttgta ttccagattc gaaagcagca attcccccat tgcccgcatc acgaaaaaga
     7501 gcataaaacc aatgatcata taaacgaaaa tgatcgacgg cccggcaagg ctaatcgttt
     7561 tgccggaccc cataaacaac ccagtaccaa tggcacctcc aatggcaata agctgaacat
     7621 gtcggtttgt gagattgcgc cgtagcgact gttcagccgg agcctgatca tcggcaacga
     7681 cttttacctg atctaccatg ttttttcttc ctgtacctgt ctgtgttgtt caggctctac
     7741 ggcctttagt cagtctatga tacgacgaat ctgtatcggg actcatcgat attaggtaac
     7801 aatacgcggg atgaatacta agatttagat ctaatgttaa ttttatgttt aaagtgagtg
     7861 tcatatcacg gtgataacgc gaaacagctc acaaaaatac acgcttacgg ggtctttgca
     7921 acacaaaata gcgccgtttg gcaaactgaa gggtttattg ctgaatgcct gctcccctct
     7981 ggttcgaggg gaacagaatg cgctgcttag aggatttcca gcagttcgac ttcaaacacc
     8041 agggtgctga acggagggat ggatgcacct gcgccgcgct cgccatatgc cagttcctgc
     8101 gggatagtca gttcccattt agaacctacc ggcatcagag tcagtgcttc aatccagcca
     8161 gggatcacgc cattaaccgg gaattcagcg ggttcaccac gagcaacgga gctgtcaaac
     8221 acggtgccgt cgatcagttt accggtgtaa tgaacacgaa cgcggtcggt acgtgccgga
     8281 attgcgcctt caccctggtt gatcacgcgg aattgcaggc cagattcggt gctattcaca
     8341 ccttcttttt tggcgttttc ttccaggtat ttcacgcctt cagcagccat cgcctggaaa
     8401 cgctgacgac gaacggcatc ggcgcgttcg tggatttcac gcagcgcgcg atgcaccaca
     8461 tcaaccggca cagccggatg tttgccttcc agcgcatcgg caatacctgc aaccagtgct
     8521 tctggcagca gcccttccag gccagattca ctcagttgtt gccctacctg caaaccaatg
     8581 ccgtagcttg cttgcgcttc gatggtgtca a
//
LOCUS       DAZXUK010000171         8428 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZXUK010000171 DAZXUK010000000
VERSION     DAZXUK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8428
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.171"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..436)
                     /gene="murD"
                     /locus_tag="ACH3L8_002982"
     CDS             complement(<1..436)
                     /gene="murD"
                     /locus_tag="ACH3L8_002982"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFT4086005.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPAL"
     gene            complement(439..1521)
                     /gene="mraY"
                     /locus_tag="ACH3L8_002983"
     CDS             complement(439..1521)
                     /gene="mraY"
                     /locus_tag="ACH3L8_002983"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFT4086006.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(1515..2873)
                     /gene="murF"
                     /locus_tag="ACH3L8_002984"
     CDS             complement(1515..2873)
                     /gene="murF"
                     /locus_tag="ACH3L8_002984"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFT4086007.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(2870..4357)
                     /gene="murE"
                     /locus_tag="ACH3L8_002985"
     CDS             complement(2870..4357)
                     /gene="murE"
                     /locus_tag="ACH3L8_002985"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFT4086008.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(4344..6110)
                     /gene="ftsI"
                     /locus_tag="ACH3L8_002986"
     CDS             complement(4344..6110)
                     /gene="ftsI"
                     /locus_tag="ACH3L8_002986"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFT4086009.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(6126..6491)
                     /gene="ftsL"
                     /locus_tag="ACH3L8_002987"
     CDS             complement(6126..6491)
                     /gene="ftsL"
                     /locus_tag="ACH3L8_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFT4086010.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(6488..7429)
                     /gene="rsmH"
                     /locus_tag="ACH3L8_002988"
     CDS             complement(6488..7429)
                     /gene="rsmH"
                     /locus_tag="ACH3L8_002988"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFT4086011.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(7431..7889)
                     /gene="mraZ"
                     /locus_tag="ACH3L8_002989"
     CDS             complement(7431..7889)
                     /gene="mraZ"
                     /locus_tag="ACH3L8_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFT4086012.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         1989 a         2429 c         2094 g         1916 t
ORIGIN      
        1 tcaacgcagg caggccaata ttgccaccca caccaacgtt aacccccgcc gctttcgcca
       61 tttcacccac tagcgtggtg accgtgcttt tgccgttaga accggtaatc gccacaatcg
      121 gtgcttgtgc ttcgcgacaa aacagctcga tatcgccaac gatttcgatt ccggcatcag
      181 cggcagcgct taaggatgga tgcgccagtg caataccggg actggcgaca atcagatctg
      241 ccgccatcag ccattcatca ttcagactgc ccgtgtggcg ttctacggct tcgggtaatt
      301 tatccaggcc aggcggtgtc atacgcgtat ccataacgcg cggcgtcaca ccgcgagcga
      361 ggaaaaagtc cacgcaggaa agcccggtga ggcccaggcc gataatgaca acatttttac
      421 cctggtaatc agccatgatt aacgtacctt cagcgttgcc agaccaatca ggaccagcat
      481 cagcgaaata atccagaaac gcacaatgac gcgcggttcc ggccagcctt tcagttcata
      541 gtggtgatga atcggtgcca tgcggaaaat acgttgtccg cgcagtttaa aggagccgac
      601 ctgcaggatg acagaaagcg tttctaccac gaacacgccc cccataatca ccagcaggaa
      661 ttcctgacgt agcagtacgg cgataatgcc taacgcacca cctaacgcca gcgaaccgac
      721 atcgcccata aagacctgcg ccggataggt gttaaaccac aggaagccca gtcctgcccc
      781 gactatcgcg gtacagacaa taaccagttc cccggcgtgt cgcagatacg gtatgtgtaa
      841 atagctggca aagttcatgt tgccggtcgc ccacgccacc agcgcaaaac caccggcgac
      901 aaatacggtc ggcataattg ccagaccgtc aagaccatcg gtcaggttta ccgcgttgcc
      961 agtacccaca atgacgaagt aagccagcag aatgtagaac agccccagtt gcggcatcac
     1021 atctttaaag aatgggacca ccagctgcgt tgcgggcgtg tctttgccgg caaggtacaa
     1081 agcgaaagca acacccagcg caatgaccga catccagaaa tatttccaac gagcgatcaa
     1141 ccctttggtg tctttacgca ccactttgcg ataatcatca acaaagccaa taacaccgta
     1201 acctaccagc accaccaaca cgcaccagac gtacggattg gacgggtaag cccacagcag
     1261 cacggagatc acaatcgctg tcaggatcat aatcccgccc atggtcggcg taccgcgctt
     1321 gctgaagtgt gattcaggac cgtcgttacg cactacctga ccaaaggaaa gtttttgcaa
     1381 atgagcaatc atgcgcgggc ccatccacaa tgagatgaac agcgcggtca gcaggctgac
     1441 gatggcgcga aacgtcagat aggaaaagac gttaaagccg gaataatatt tgaccaaatg
     1501 ttcggccagc caaactaaca tgtcccattc tcctgtaaag cgcgtactac ctcttccatg
     1561 gcagcactac gtgaaccctt aactaaaatc gtaattacct gttgctcagc aatcagtgat
     1621 ttaagacgcg taattaacgc agttttatca gcaaaatgtt cgccaacgcc gctggcggtg
     1681 ctgatagcat ggctttgttt acccatgctt aacacgcggt caataccagc agcttttgcc
     1741 gcctcaccca cctgtacatg gcaggcttcg ctttcagcgc ccagttccgc catatcgccc
     1801 accaccagca cgcggtagcc cggcatttca gccagtacct gaacagctgc ggtcattgaa
     1861 ccgacattgg cgttgtagga gtcgtcgagc agcaactggt tttctgccag ttggatgggg
     1921 aacagacggc caggaacagc tttcagattt gccagccccg ctttgatagc atcaagcgtt
     1981 gcgcccacgg acatggagag cgcagcggct gccagcgcat tcgcaatatt gtgacgcccc
     2041 ggcaacggca gcagaacatc gacgctaccg gttggggttt gtagggtaaa ttccataccg
     2101 tgcgaggtca catggatatt ggtggcggtg aaatcgctgt tggcggcatt gggtgagaaa
     2161 cgccacactt tgcgtgagcc aattacgctc tgccagttca gccagtcgtt gttgtcggcg
     2221 ttcataatgg cgataccgtt ttccggcagg ccgctaaaga tttcaccttt cgctttcgcg
     2281 acacccgcaa gcgagccaaa accttccaga tgcgccgctg ccaggttgtt gaccagcgcc
     2341 gcttccgggc gagtcagact cacagtccag gcaatctcgc cctgatggtt cgcgccaagt
     2401 tcaataactg cgtaatcgta ttccggcgtt aagcgcaaca gcgtcatcgg tacaccgatg
     2461 tcgttgttga gattgcctgc cgtataaagc gtgttgccgc actggcttaa aatcgccgcc
     2521 gtcatctctt taacggaggt tttgccggag gaccccgtca gagcaaccac gagcgccgga
     2581 acttgctggc gaacccatgc agccagttca ccaaacgcca gacgcgtatc cttgacgatt
     2641 aactgcggca ggtcgatgtc cagcggacgg ctaaccagta gtgcgcctgc gccgccagct
     2701 ttcgcctggt cggcaaaatc gtgggcatca aaacgttcac ctttcagggc aacaaacagg
     2761 cagcccggcg tcagttttcg ggtatcagtg gttacagcat caagggtgat atctgcacct
     2821 tgcagttcac cgttgagaat atcggtaagt tggctaaggg ttacgctaat catgcaatcc
     2881 cccccagcag acgcgccacc gtgacgcgat cggagtagtc cagacgctga ttgccaacaa
     2941 tctggtaatc ctcatggcct ttgcccgcga ccagtaccac atcattctct ttagcctgca
     3001 taacggcgca agtcaccgct tcagcacggc cttccatcac tttggcatgt ccggcatcta
     3061 acattcccgc cagaatatcg ttgatgatgg cacgcggttc ttcggtacgc gggttatcgt
     3121 ccgtcaccac cgccacgtca gcaaactctt cggcaattgc gcccatcagt ggacgcttac
     3181 ctttatcgcg atcgccacca cagccaaaga cgcaccacag cttgcccgca cagtgcaggc
     3241 gcgccgcctg taaggctttt tccagtgcat ccggcgtatg cgcgtaatcc accaccaccg
     3301 tcggtttgcc tggcgcagtg aacacttcca tacgtccgca aaccggttgc agacgcgcgg
     3361 cggttttcag cagatcagcc agtggatagc ccagcgccag cagtgttgcc agcgcgagca
     3421 acaggttgct gacgttaaaa gcgcccatca gatggctttc aatttcgcca tcgccccagc
     3481 ttgaactaaa gcgaatggtc gcgccgctgt cgtgatagtt cacttcggtc gctttcaacc
     3541 agcgtccgtg acagttcgga ttgatatgat cttccattga taccgcaacc gcgtccggca
     3601 gttttgccag ccagcggcgg cccacttcat cgtcggcgtt aataatcgcc tgaccgcaat
     3661 gatgttcaga ataaagcagc catttcgcag cttcgtagtg ttccatatca ccatgataat
     3721 caaggtgatc gcggcttaag ttggtaaaga ccgacgccgg aaatttcaat gccgccacac
     3781 ggtgctgtac cagcccgtgg gaggaaactt ccattgcgca aaaagtcgcg ccctgatcca
     3841 ccagccccgc cagctcatgc tgaacatcaa ctgccgaacc ggtagtattt tctgtcggga
     3901 tcactttccc cagcagaccg ttaccaacgg tgcccattac cgcgctggtt tcgccaagca
     3961 gttggctcca ctgcgccaga agctgggtcg tcgtggtttt gccgttggtg cccgttacgc
     4021 ctacgagacg taaattgtca gagggttcat ggtaaaagcg gcccgccagt gcagataaac
     4081 gctcgttgag ctggctgaga tagatgaccg gtacgccgtg catttcacgg atttcaccgt
     4141 cggtcgcctc atctttcgcc tctgcaataa tggcagccac accttgcgct atcgcctgcg
     4201 ggatatatcg acgcccgtcc gcctgatgac ctactacagc tacaaagaga tcgcccgccg
     4261 cagccacacg gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg tctggcaccc
     4321 acggagcaag aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc ttgattaatc
     4381 acaaattcat ttttatcgcc cgttgtcagc gcatccggct cgatgttcat ggtacgcaat
     4441 acgccgccca tgatggcacc aaagaccggc gcggaaacgg cgccgccgta gtatttaccc
     4501 gcctgcggat cgttgataac aacaaccagc gcgaagcgcg gctgactcgc aggcgcaacg
     4561 cctgcggtat aagcaatata tttattgatg tagcgaccgt ccggcccgac ctttttcgcg
     4621 gtaccggttt taatggcgat acgatagcct ttaatcgccg ccttcacgcc gccgccgcct
     4681 ggtagcgcca cgctttccat catatgcacc acggtgcgaa caatggattc cgggaagaca
     4741 cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca gtgggcgata aatgccgtag
     4801 ctgccgatag ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag cccgtagccg
     4861 aaagagaagg tggccctctc tatgtcagac caccgttgtt tttgaggata taagccactg
     4921 cgttctccga ccaaccccaa attggtcgct tttcccagtc caaaacgtga gtaagtatct
     4981 actaacgctg aggacggcat cgttaacgcc agcttggaaa caccgacgtt actcgacttc
     5041 tgtaataccc cggtcagggt taattcgctg tagcgtgcca cgtctttgat ttcgtggccg
     5101 ttaattcgat aaggaatggt attgagtacc gagttttccc gcaccacgcc acgttgcaac
     5161 gcggtcatca ccaccatcgg tttaaccgtt gagcccggtt caaacacgtc ggtgatggta
     5221 cggttacgca tcgcctcttt cggcgtgccg ctcagattgt tagggttgta tgacgggctg
     5281 ttagccatcg ccagcacttc acctgtgttg acatccacca gcacggcgct accagattca
     5341 gccttgttaa aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg caggcgttca
     5401 tcaatactca gcgccaggtt gtgcgctgcc tggctgtcag tagaagaaat atcttcaatt
     5461 acgcgaccat agcggtcttt acgcacaatg cgctcacccg gctgcccggt aagccattta
     5521 tcgaaactct tctcaacgcc ctcaatccct tgactatcga cgttagtaaa gccgatgagg
     5581 tgagcagtca cttcgccgga cggatagtaa cggcgagact cttcacgcag atgaatcccc
     5641 ggcagtttca gttttttgat gtagtccgcc atgtcagagt ttacctgacg cgccagataa
     5701 ataaagcgcc ctttcgggtt ggcgttaatg cgggcagaaa gctgatccag cggaatattg
     5761 agcgcgttag ccagcgcctt ccagcggtca ccgacgctga taccgccagc gtcatgcact
     5821 tctttcgggt cagcccaaat cgcttttacc ggcacgctca ccgctaacgg gcgaccagaa
     5881 cggtcagtaa tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga acgcatgtcg
     5941 ccctctttca ccagcatatc cggggagata acttgtaacc acgctacgcg tccgagcaga
     6001 aaagccagcg ccaggagaat acagccgcat aacaacgcaa aacgccaact gataaagttg
     6061 gcatgttctt cctgacgttt tggtttctgc gttttcgccg ctgctttcat gcgtcgcgtt
     6121 tatccttatt tttgcactac gatattttct tgtgacggat caacatgctg catttgcagc
     6181 ttttccgtgg cgatcctttc cacccggcta tggtcgccga gcgcattctc ttcaaggatc
     6241 aggttgcgcc attcaatgtc taaagcatct cgctccagca ccagttgttc gcgctgagcg
     6301 gtcagtaaac gggtatggtg cgccgtggtt accacggtca ccgccgtcaa aataatgcaa
     6361 atgaacaggc agagtggcag cttcccaaat cgcaaaagat cgtcaccgat aacaccaggc
     6421 aatgcatggc gctcgtggct tcccatcgat cctttaactt tgcttagagc ttctgtcact
     6481 ctgctgatca tgcgttcgtc ctctctgcaa tacgcagaac tgaactacgg gcacgagggt
     6541 tctcagccac ctcttcttcg cccggcatta acttgcctag tgctcgcagc tgacggccac
     6601 ccagtttttt gagctgctct tcagtcatcg gtaaccctgc cggaacttgc ggaccgcggc
     6661 tgttttcacg cataaaacgt ttcacaatac ggtcttccag cgagtggaag ctgatgatcg
     6721 aaagccgccc acccggggcc agcacgttga gcgagctttt tagcgcctgc tctatctcct
     6781 ccagttcact gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg ggatgtttaa
     6841 acttatcttt caccggcgtt gcagcagcca cgacttccgc cagttctttg gtgcgggtca
     6901 tcggctgttc gcggttacgc tcgacaatgg cgcgggcaat gcgtttggca aaatgctctt
     6961 caccataggt tttcaatacc caggcgatat cggcttcttc tgcggtttgt agccattcag
     7021 cggctgactg cccacgggtt gggtccatac gcatgtccag cggaccatcg cgcataaagg
     7081 aaaagccgcg ttcagcatca tcaagttgcg gtgaagagac gccaagatcg aggagaatgc
     7141 cgtcgatctt gccaataaga tcgcgctcgg caacgtattc gcccagcgcg gagaaaggtc
     7201 cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata gcctgcgggt
     7261 cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg cgagaggatc agacgtgagt
     7321 gaccaccgcg accaaaagtc ccatcaatgt agatgccatc aggacggata ttgaggccat
     7381 taacggcttc atccagcagc accgtagtat gtttatagtt ttccatcatt ttatagagac
     7441 aagtcctgca gtcgctccga taagtctccg gtagccaact gctctgcgtc gatatcttcc
     7501 ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa acttgttgaa ctgtccaacc
     7561 agcatcactt cttttgtcag cccggcatgt tgccgcagta ctggcgcgat taacaatcga
     7621 cctgcgccat ccatctgaca ttcgctggca tgacctaaca gtaggcgctg cacacggcgc
     7681 tcaaccgggt tcatgctcaa cagacgcgat aatttttgct cgataatttc ccattcaggc
     7741 agggggtaaa gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat ttgaccggca
     7801 gcgttctcaa gcagctgttc ccgataacgg gtaggcactg ataagcgccc tttgctgtcg
     7861 agattgacta acgttgctcc ccggaacatg ccagcctcac cccttattcc cactttgccc
     7921 cacaatttcc cactgaaagg agtttacgga gcggaggaaa agcttgtcaa gccagtcaca
     7981 aggcataccg aggtaaaaaa cccgatgttt acggctaata tcagcgttgt ttattttacg
     8041 tccaacgtgc gaagcaaatt aacgttatga atagctgtaa gaaaaaatcc ctcatcgtca
     8101 tcactgtttt aaaatgactc actattttcc acagcaaata taaagtgtca gtttgcgacg
     8161 cgagcggcat tttaggacat atcttccccg gttaacagtc cttgttacgt ctgtgtggcg
     8221 cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgt ttgttaaatc ggcaattcat
     8281 cgccgcatgt aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat tattgtgaat
     8341 ttcagctggt gagttaatta acgggcgggt ttaaaaccat ttatcaataa tagtcaggaa
     8401 tagtcttatt tactttaagg atattgat
//
LOCUS       DAZXUK010000172         8361 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZXUK010000172 DAZXUK010000000
VERSION     DAZXUK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.172"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..840
                     /locus_tag="ACH3L8_002990"
     CDS             <1..840
                     /locus_tag="ACH3L8_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HFT4086013.1"
                     /translation="NFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLID
                     TGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLHW
                     RKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTFG
                     EGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEISA
                     TVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRKE
                     KAQ"
     gene            996..2441
                     /gene="tldD"
                     /locus_tag="ACH3L8_002991"
     CDS             996..2441
                     /gene="tldD"
                     /locus_tag="ACH3L8_002991"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFT4086014.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(2569..3498)
                     /gene="aaeR"
                     /locus_tag="ACH3L8_002992"
     CDS             complement(2569..3498)
                     /gene="aaeR"
                     /locus_tag="ACH3L8_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFT4086015.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            3681..3884
                     /gene="aaeX"
                     /locus_tag="ACH3L8_002993"
     CDS             3681..3884
                     /gene="aaeX"
                     /locus_tag="ACH3L8_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFT4086016.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            3892..4824
                     /gene="aaeA"
                     /locus_tag="ACH3L8_002994"
     CDS             3892..4824
                     /gene="aaeA"
                     /locus_tag="ACH3L8_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFT4086017.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            4830..6797
                     /gene="aaeB"
                     /locus_tag="ACH3L8_002995"
     CDS             4830..6797
                     /gene="aaeB"
                     /locus_tag="ACH3L8_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFT4086018.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            6889..7161
                     /locus_tag="ACH3L8_002996"
     CDS             6889..7161
                     /locus_tag="ACH3L8_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFT4086019.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(7217..7480)
                     /gene="yhcN"
                     /locus_tag="ACH3L8_002997"
     CDS             complement(7217..7480)
                     /gene="yhcN"
                     /locus_tag="ACH3L8_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFT4086020.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(7845..8315)
                     /gene="argR"
                     /locus_tag="ACH3L8_002998"
     CDS             complement(7845..8315)
                     /gene="argR"
                     /locus_tag="ACH3L8_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFT4086021.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2002 a         2016 c         2239 g         2104 t
ORIGIN      
        1 aacttccgtg gctggccgga cgcacaaata cgatgcacag agtgctggtt ctgggggcaa
       61 aaattcggtc gcattgacag tgatatcacc atttctggcg atacgttaac gctgaccaat
      121 ggactgattg atactggttt ctcgcggctt actgccgatg gtgaatgggt taataatccg
      181 gggaatgaac gtacctcgct gaaaggaaaa ctgcgtgggc agaaaattga tgccgccgca
      241 gaattttttg gtgtcacgac gcccatacgc cagtcgtcat ttaatgtgga ttacgattta
      301 cactggcgca aagcaccctg gcagccagat gaagcgacgt tgaatggcat cattcatact
      361 caactgggta aaggcgaaat taccgaaatc aataccggac atgccgggca attgctgcgc
      421 ttattgagcg tagatgccct gatgcgtaag ctgcgttttg atttcagaga cacttttggc
      481 gaagggttct attttgactc cattcgcagc accgcgtgga ttaaagacgg cgttatgcac
      541 accgacgaca cgctggtgga tggcctggag gcggatatcg ccatgaaagg gtcggtaaat
      601 ctggtacgcc gcgacctgaa tatggaagcg gttgtcgcac cagagatttc tgcgacggtg
      661 ggcgtggctg cggcttttgc ggttaacccc attgttggcg cggcagtgtt tgccgccagt
      721 aaagtgctgg ggccgctgtg gagcaaagtc tccattttgc gctatcacat ttcgggtccg
      781 ctggacgatc cgcaaatcaa cgaagtgttg cgccaaccgc gtaaagaaaa agcgcaatga
      841 tttgacgagg gcgcgtaatt gccccaatct catatgataa tcgttgccaa aggccaacga
      901 gccggaacat aactgtaggc cggatagggc gttcacgccg catccggcag ccgtaaaaaa
      961 tcctctactg cagtaactaa cgagtagcaa aaacgatgag tctgaacctg gtaagtgaac
     1021 aattgctagc ggcgaacggc ctgaaacatc aggacttgtt cgcgatcctc ggtcaactgg
     1081 ccgaacgtcg ccttgattat ggcgatctct attttcagtc gagctatcac gaatcctggg
     1141 ttttagaaga ccgcattatt aaagatggtt cttacaacat cgatcagggc gttggcgtgc
     1201 gtgcaatcag cggtgaaaaa accggatttg cttacgctga ccaaatcagc ctgctggcgc
     1261 tggaacagag tgcgcaagcg gcgcgcacca tcgtccgtga tagtggtgat ggcaaagtac
     1321 agacgctggg cgcggtagag catagcccgt tgtatacctc ggtagatccg ctgcaaagca
     1381 tgagccgtga agagaagctg gatatcctgc gtcgcgtcga taaggttgct cgcgaagcgg
     1441 acaagcgcgt acaggaagtg actgccagcc tcagtggtgt ctatgaatta attctggttg
     1501 cggccaccga cggcacgcta gcggcggatg tccgtccgct ggtgcgtctt tccgtgagcg
     1561 ttctggtcga agaagatggc aaacgcgaac gcggtgccag tggcggcggc ggtcgttttg
     1621 gttatgagtt cttccttgcc gatcttgacg gcgaagtccg tgcggatgca tgggcaaaag
     1681 aagcagtgcg tatggcgctg gtcaatcttt ctgccgttgc tgcaccagcg ggcaccatgc
     1741 cggtagtact tggcgcaggt tggccgggcg tgctgttgca tgaagcggta ggtcacggtc
     1801 tggaaggcga cttcaaccgc cgtggcactt cagtatttag tggacaggtc ggggagctgg
     1861 tggcttcaga actgtgtacc gtggttgatg acggcacgat ggtcgatcgc cgtggttcgg
     1921 tggcgattga tgatgaaggt acgccaggcc agtacaacgt gctgattgag aacggcattc
     1981 tgaaaggcta catgcaggat aaactcaacg cgcgtttgat ggggatgacg ccgactggca
     2041 acggtcgccg tgaatcctac gcccatctgc ccatgccgcg tatgaccaac acctatatgc
     2101 tgccgggtaa atcgaccccg caagaaatta ttgaatccgt tgagtacggt atctatgcac
     2161 cgaactttgg tggcggtcag gtggatatca cctccggcaa attcgttttc tccacttcag
     2221 aagcatatct gattgaaaac ggtaaagtaa cgaagccggt gaaaggcgca acgttgattg
     2281 gttccggtat cgaaaccatg cagcagattt cgatggttgg caacgacctg aaactggata
     2341 acggcgtggg tgtctgcggt aaagaagggc aaagtttgcc ggttggcgtg ggccagccaa
     2401 cgttgaaagt cgataacctg actgttggcg gtactgcgta ataatttcat tattttcaga
     2461 aggataatta atctttctac gtgcacgaac ggtcccctcg ctcctctggg gttagggtga
     2521 ggggaacccg ttggcacagg tttgtagaac gtaacagtac aatatgaatt acttctcttt
     2581 cccgcgcccg tgcatctcct gaaacaattt accgacctca acaaaataat ccgtcagcga
     2641 gttgatcacg acctgtacct tcagcggcag cttatctttt tcggtatata acgcataaac
     2701 cgggcgtgga tctgactgat aacgcggcag caggatctcc agctccccac gattgatctc
     2761 gttgatcacc cacatcagcg gcacgtaggc gatcccggca cccgccgtta gccagcgcac
     2821 cagcgtcatc ggatcattag tcacaaatct cccttgcggg atcaggcgag tcgagatccc
     2881 ttccggtgca atcagttcaa attcattgtc gggccgcacg ctgtattcaa gccatgaatg
     2941 gctacttaaa tcggcgggtt tttccggtat gccgtattgt gtgagatagc ttttcgcggc
     3001 gcacaccacc attggcatcg cgccaagacg gcgggaaaac aggctggaat cctgcaacgc
     3061 gccgacgcgg atcaccacat ccagaccgtc ggcaatcagg tcgggggctg gaattccggt
     3121 aaccagattg gcgctcaaac ctgggtattc tttcagcatt ttggctgtca gcccggcgag
     3181 aacattttgt gccatagttg aagaacagcc aatgcgtagc gtcccgatgg gggtgttatt
     3241 gaaggcatac agttgctcat gaacatcctg cacttcatga agcatacgac ggcagccctg
     3301 gtagtaaatt ctaccggctt cggtcaggcc aatgctgcgt gtgctacggt ttaacagctt
     3361 tacctgcaac tcatcttcca gttttgacac cgtctgactg atggacgaaa cgctcatctg
     3421 tagctgtctg gcggcggcgg taaaagagcc aaattcaact actttggcaa acaccgacat
     3481 gcgttttagt cgttccatta ttcactctga cttaaaagtg atttagatca cataatatag
     3541 ataacagcat aacagttacg ctaatatatt aaatatcaat ctacagcaat gttgctctcg
     3601 cccggctttc catgcccttc tctgcggcga cagatgctga aaataataac gcctgctctc
     3661 tctatacaac caaggtcaac atgagtctgt ttcccgttat cgtggtgttt gggctgtcct
     3721 tcccaccgat attttttgaa ttgcttttat cactggcgat tttctggctg gtgcgccggg
     3781 tacttgtgcc aacaggtatc tacgactttg tctggcatcc ggcgttgttc aacaccgcgc
     3841 tctattgctg cttgttttat ttgatatcgc gactgttcgt ttgaggttga agtgaaaaca
     3901 ctaataagaa aattctcccg tacggccatc acggtcgtat tagtcattct ggccttcatc
     3961 gcaattttta atgcctgggt ctattacacc gaatccccct ggacgcgtga cgcgcgcttt
     4021 agcgctgacg tcgttgcgat cgcgccggac gtttctggac tcattaccca ggtgaatgtt
     4081 catgataacc agctggtgaa aaaaggacag atactgttca ccatcgacca gccgcgctat
     4141 caaaaggcgc ttgaggaagc gcaagccgat gttgcttatt atcaggtact ggcacaggag
     4201 aaacgccagg aggccggacg tcgtaaccgt ctcggtgtgc aggcgatgtc tcgcgaagag
     4261 atcgaccagg ctaacaacgt actacaaacg gttctgcatc agttagcgaa agcgcaggcg
     4321 acccgcgatc tggcaaaact ggatcttgaa cgcacggtga tccgagcgcc agcagatggc
     4381 tgggtgacca acctcaacgt ctataccggt gagtttatta ctcgaggatc aacggcggtt
     4441 gcgctggtga aacagaactc cttctatgta ctggcctata tggaagaaac taagctggaa
     4501 ggggtgcgtc cggggtatcg tgcagagatc acgccgcttg gcagtaacaa agtgctgaaa
     4561 gggactgttg atagtgttgc cgctggggtc accaacgcca gcagcacgcg tgacgacaaa
     4621 gggatggcga ccatcgactc tgaccttgaa tgggtgcgtt tggcgcaacg tgttccggtt
     4681 cgtattcgtc tcgacaacca gcaagagaac atctggcctg cgggcaccac tgctacagtg
     4741 gtggtcactg gcaaacaaga tcgcgacgaa agccaggatt cgttcttccg taaaatggcc
     4801 catcgcctgc gtgagtttgg ttaatcacga tgggtatttt ctccattgct aaccaacata
     4861 ttcgctttgc ggtaaaactg gcgaccgcca ttgtactggc gctgtttgtt ggctttcact
     4921 tccagctgga aacgccacgc tgggcggtac tgacagcggc gattgttgcc gccggtccgg
     4981 cctttgctgc gggaggtgaa ccgtattctg gcgctattcg ctatcgtggc tttttgcgca
     5041 tcatcggcac atttattggc tgtattgccg gactggtgat catcattgcg atgatccgcg
     5101 caccattatt gatgattctg gtgtgctgta tctgggccgg tttttgtacc tggatatcct
     5161 cgctggtacg aatagaaaac tcgtacgcgt gggggctggc cggttatacc gcgctgatca
     5221 ttgtgatcac cattcagccg gaaccattgc ttacgccgca gtttgccgtc gaacgttgta
     5281 gcgagatcgt tatcggtatt gtgtgtgcga ttatggcgga tttgctcttt tctccgcgat
     5341 cgatcaaaca agaagtagat cgagagctgg aaagtttgct ggtcgcgcaa tatcaattaa
     5401 tgcaactctg tatcaagcat ggcgatggtg aagttgtcga taaagcctgg ggcgacctgg
     5461 tgcgacgcac cacggcacta caaggcatgc gcagcaacct gaatatggaa tcttcccgct
     5521 gggcgcgggc caatcgacgt ttaaaagcga tcaatacgct atcgctgacg ctgattaccc
     5581 aatcctgcga aacttatctt attcagaata cgcgcccgga attgatcact gatactttcc
     5641 gcgaattttt tgacacgccg gtagaaaccg cgcaggacgt ccacaagcag ctcaaacgcc
     5701 tgcggagagt tatcgcctgg actggggaac gggaaacgcc tgtcaccatt tatagctggg
     5761 tcgcggcggc aacgcgttat cagcttctca agcgcggcgt tatcagtaac acaaaaatca
     5821 acgccaccga agaagagatc ctgcaaggcg aaccggaagt aaaagtagag tcagccgaac
     5881 gtcatcatgc aatggttaac ttctggcgaa ccacactttc ctgcattctg ggcacgcttt
     5941 tctggctgtg gacgggctgg acttccggca gtggtgcaat ggtgatgatt gcggtcgtga
     6001 cgtcactggc aatgcgtttg ccgaatccac gcatggtggc gatcgacttt atctatggga
     6061 cgctggccgc gctgccgtta gggctgctct actttttggt gattatccct aatacccaac
     6121 agagcatgtt gctgctgtgt attagcctgg cagtgctggg attcttcctc ggtatagaag
     6181 tacagaaacg gcgactgggc tcgatggggg cactggccag caccataaat attatcgtgc
     6241 tggataaccc gatgactttc catttcagtc agtttctcga cagcgcatta ggacaaatcg
     6301 tcggctgtgt gctcgcgttc accgttattt tgctggtgcg ggataaatcg cgcgacagga
     6361 ctggacgtgt actgcttaat cagtttgttt ctgccgctgt ttccgcgatg actaccaatg
     6421 tggcacgtcg taaagagaac cacctcccgg cactttatca gcagctgttt ttgctgatga
     6481 ataagttccc aggggatttg ccgaaatttc gcctggcgct gacgatgatt atcgcgcacc
     6541 agcgcctgcg tgatgcgccg atcccggtta acgaggattt atcggcgttt caccgacaaa
     6601 tgcgccgcac agcagaccat gtgatatctg cccgtagcga tgataaacgt cgtcggtact
     6661 ttggccagtt gctggaagaa ctggaaatct accaggaaaa gctacgcatc tggcaagcgc
     6721 caccgcaggt gacggaaccg gttcatcggc tggcggggat gctccataag tatcaacatg
     6781 cgttgaccga tagttaagtc aaaaccgacg ccaaaagcgt cggttttttc atggctatac
     6841 ttagcgatgc acggcagaac tcgccgcgaa acgtgacggt ggcaacagat gaatatttat
     6901 gcctttgatt ttgatgagat tgagagtcag gaggattttt atcgtgactt tagccaaacc
     6961 tttggtctgg ctaaagataa ggtacgcgat ctcgactcac tatgggatgt gttaatgaac
     7021 gatgtcctgc cgctaccact tgagattgaa tttgttcatc tgggagagaa aacgcgtcgc
     7081 cgttttggcg cgttaatatt gctgtttgat gaggcagagg aagagctgga agggcatttg
     7141 cgttttaatg ttcgtcatta gcgcaaaaaa aagcccacga accgggggca atatcgtcgg
     7201 acaaaacgat gagggtttat ttgtacagtt cagccgtagc gtgccaggtg tcaccgctac
     7261 gagcttcagt aatctggtag gccgttgcgc ctttctcttc cgcttttttg ttcagcattt
     7321 cacgtatatc cattggcgaa gacgccacac cacttacgga tacggtcccg attgcttcac
     7381 gattttgtgc ttgtgcagca tcaatggagt cggcagcgaa tgcaccgaaa gagagaacag
     7441 aaagtacgct taatgcagct acagtggttt tgattttcat gatttttacc tcgacataat
     7501 cttttagctg ggcctttgtt tcgtgaccca tatcacaaaa tcaagtatac actaattatt
     7561 gaactaatta ataccacact aactgtttca gttgatacta agtgttaaaa atgtgaattt
     7621 taataacaaa aaagtataaa aaacacggtt atttattatt aattatagta ttttcaatta
     7681 gataatgaat tttgcataat gtgctttttt taccgtatgt attcattttg tgaatgatgt
     7741 gtcgcggtaa aacgcactat tcgttaaatg tatcgcagga gaaagaaggg gaatgagagg
     7801 gataagcaac attttccccg ccgccagaag cgacggggca gagattaaag ctcctggtcg
     7861 aacagctcta aaatcgcttc gtacaggtct ttgacggtga aaccgttagc aggggtggta
     7921 aagatggtgt catcgccagc gatggtgccc agaatacctt ctgctttgcc cagtgagtcc
     7981 agcaggcgag caattaactg cgccgcgcca gggctggtat gaatcacgac aactgcatcg
     8041 ttgtagtcga tatccagcac cagattcttc aatggactgg aggtggttgg tacacccagt
     8101 tcagctggca ggcagtaaac catttccatt ttggcattgc gtgtacgtac agcaccaaac
     8161 ttggtcaaca tccgcgagac tttagactga ttaatattgt caaagccttg ctcctgcaac
     8221 gcggcgacga tttcgccctg ggagctaaat ttctcttctt taagtaatgc tttaaatgct
     8281 ttaactagtt cttcttgctt agccgagctt cgcataagtc acccgatatg gtggttgata
     8341 caacattatt gtgcatacag a
//
LOCUS       DAZXUK010000173         8332 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZXUK010000173 DAZXUK010000000
VERSION     DAZXUK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.173"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="ACH3L8_002999"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="ACH3L8_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACH3L8_003000"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACH3L8_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFT4086022.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACH3L8_003001"
     CDS             complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACH3L8_003001"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFT4086023.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACH3L8_003002"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACH3L8_003002"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFT4086024.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="ACH3L8_003003"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="ACH3L8_003003"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFT4086025.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7783..7858
                     /locus_tag="ACH3L8_003004"
     tRNA            7783..7858
                     /locus_tag="ACH3L8_003004"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7816..7818,aa:Gly,seq:gcc)
     gene            8016..8091
                     /locus_tag="ACH3L8_003005"
     tRNA            8016..8091
                     /locus_tag="ACH3L8_003005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8049..8051,aa:Gly,seq:gcc)
     gene            8127..8202
                     /locus_tag="ACH3L8_003006"
     tRNA            8127..8202
                     /locus_tag="ACH3L8_003006"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8160..8162,aa:Gly,seq:gcc)
     gene            8238..8313
                     /locus_tag="ACH3L8_003007"
     tRNA            8238..8313
                     /locus_tag="ACH3L8_003007"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8271..8273,aa:Gly,seq:gcc)
BASE COUNT         1907 a         2151 c         2258 g         2016 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagctc
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagata
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa
     7861 atttgaaaag tgctgcaaag cacagaccac ccaatcgcag tatttcgaat tgcgacaagg
     7921 cggcaactga acgaatcctt gggaacttac aaaagtatgt gactaaggtg agtgaaggca
     7981 gccaaggcag tagcggttcg aaagacgaag attaagcggg aatagctcag ttggtagagc
     8041 acgaccttgc caaggtcggg gtcgcgagtt cgagtctcgt ttcccgctcc aaaatttaaa
     8101 agtgctgtaa ggcatagacc acccaagcgg gaatagctca gttggtagag cacgaccttg
     8161 ccaaggtcgg ggtcgcgagt tcgagtctcg tttcccgctc caaaatttga aagtgctgta
     8221 aggcacagac cacccaagcg ggaatagctc agttggtaga gcacgacctt gccaaggtcg
     8281 gggtcgcgag ttcgagtctc gtttcccgct ccaaattctt ctctcaataa aa
//
LOCUS       DAZXUK010000174         8323 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZXUK010000174 DAZXUK010000000
VERSION     DAZXUK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.174"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            279..1619
                     /gene="dcuB"
                     /locus_tag="ACH3L8_003008"
     CDS             279..1619
                     /gene="dcuB"
                     /locus_tag="ACH3L8_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFT4086026.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1697..3343
                     /gene="fumB"
                     /locus_tag="ACH3L8_003009"
     CDS             1697..3343
                     /gene="fumB"
                     /locus_tag="ACH3L8_003009"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFT4086027.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3466..4095
                     /locus_tag="ACH3L8_003010"
     CDS             3466..4095
                     /locus_tag="ACH3L8_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFT4086028.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(4234..5655)
                     /gene="melB"
                     /locus_tag="ACH3L8_003011"
     CDS             complement(4234..5655)
                     /gene="melB"
                     /locus_tag="ACH3L8_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFT4086029.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(5758..7113)
                     /gene="melA"
                     /locus_tag="ACH3L8_003012"
     CDS             complement(5758..7113)
                     /gene="melA"
                     /locus_tag="ACH3L8_003012"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFT4086030.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            7396..8304
                     /gene="melR"
                     /locus_tag="ACH3L8_003013"
     CDS             7396..8304
                     /gene="melR"
                     /locus_tag="ACH3L8_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFT4086031.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         2130 a         2184 c         2045 g         1964 t
ORIGIN      
        1 aaatatcaat aagataacag caaacaaaac attaacatct gcgcagtaca aactataaac
       61 ccatcgccag agagtttttc tctctgaaaa agccgcttat cacagtgcat aaatttgccg
      121 ctgctttaat cagccaatat tcactgtgag gtatttgcta aagccggtaa cgaccaaacg
      181 gatatttagt caggctctga aaacagttca tacaaaacag aacgtgactg tgatctattc
      241 agcaaaaatt taaataggat tatcgcgagg gttcacacat gttatttact atccaactta
      301 tcataatact aatatgtctg ttttatggtg ccagaaaggg tggtatcgcg ctgggtttat
      361 taggcggtat cggtctggtc attctggtct tcgtcttcca ccttcagcca ggtaaaccac
      421 cagttgatgt catgctggtt atcattgcgg tggtggcggc atcggcgacc ttgcaagctt
      481 cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct gctgcgccgc aacccgaaat
      541 atgtctcaat tgtcgcgccg tttgtgacct gtacactgac cattctttgc ggtacgggtc
      601 atgtggttta caccattctg ccgatcatct acgacgtcgc cattaagaac aacatccgtc
      661 cggaacgtcc gatggcggca agttctatcg gtgcacagat ggggattatc gccagtccgg
      721 tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa tgtcaccttt gatggtcgcc
      781 atcttgagtt cctcgatctg ctggcaatca ccattccatc gacgttaatc ggtatcctgg
      841 cgatcggtat cttcagctgg ttccgcggta aagatctgga taaagacgaa gagttccaga
      901 aattcatctc cgtaccggaa aaccgtgagt atgtttacgg tgataccgcg acgctgctgg
      961 ataaaaaact gccgaaaagc aactggctgg caatgtggat tttcctcggg gcaatcgctg
     1021 tagtcgccct tcttggtgct gattcggacc tgcgtccatc cttcggcggc aaaccgctgt
     1081 cgatggtact ggttattcag atgtttatgc tgctgaccgg ggcgctgatt attatcctga
     1141 ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt ccgttccggt atgatcgcca
     1201 tcgtggcggt gtacggtatc gcatggatgg cagaaaccat gttcggtgcg catatgtctg
     1261 aaattcaggg cgtactgggt gaaatggtga aagagtatcc gtgggcgtat gccattgttc
     1321 tgctgctggt ttccaagttt gtaaactctc aggctgcggc gctggcggcg attgttccgg
     1381 tcgcgctggc gatcggcgtt gatccggcat acatcgtggc ttcagcaccg gcttgctacg
     1441 gttattacat cctgccgact tatccgagcg atctggcagc gattcagttt gaccgttccg
     1501 gcaccaccca catcggtcgc ttcgtcatca accacagttt tattctgccg gggttgattg
     1561 gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc gatgtacggg ttcttataaa
     1621 tgcactttgc gtgccgcccg tgactacgcg gcacgccatt ttcgaataac aaatacagag
     1681 ttacaggctg gaagctatgt caaacaaacc ctttatctac caggcacctt tcccgatggg
     1741 gaaagacaat accgaatact atctactcac ttccgattac gttagcgttg ccgacttcga
     1801 cggcgaaacc atcctgaaag tggaaccaga agccctgacc ctgctggcgc agcaagcctt
     1861 tcacgacgct tctttcatgc tacgtccggc acaccagaaa caggttgcgg ctattcttca
     1921 cgatccagaa gccagcgaaa acgacaagta cgtggcactg caattcttaa gaaactccga
     1981 aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc ggcaccgcga tcatcgtcgg
     2041 taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa gaagcgctgt cgaaaggcgt
     2101 ctataacacc tatatcgaag ataacctgcg ctactcgcaa aacgcggcgc tggacatgta
     2161 caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc gacctgtacg cggtagatgg
     2221 cgatgagtac aaattccttt gcgttgcgaa aggcggcggc tctgccaaca aaacgtatct
     2281 ctaccaggaa accaaagccc tgctgactcc cggcaaactg aaaaacttcc tcgtcgagaa
     2341 aatgcgtacc ctcggtactg cagcctgccc gccgtaccat atcgcgtttg tgattggcgg
     2401 tacgtctgcg gaaaacaacc tgaaaaccgt caaattagca agcgctcact attacgatga
     2461 actgccgacg gaagggaacg aacatggtca ggcgttccgc gatgtccagc tggaacagga
     2521 actgctggaa gaggcccaga aactcggtct tggcgcgcag tttggcggta aatacttcgc
     2581 gcacgacatt cgcgttatcc gtctgccacg tcacggcgca tcctgcccgg taggcatggg
     2641 cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc aaccgcgaag gtatctggat
     2701 cgaaaaactg gaacacaatc taggtcagta cattccacaa gaactgcgtc aggccggtga
     2761 aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa gagatcctcg cccagctttc
     2821 gcaatatccg gtttccactc gtttgtcgct cactggcacc attattgtgg gtcgagatat
     2881 tgcacacgcc aagctgaaag agctgattga cgccggtaaa gaacttccgc agtacatcaa
     2941 agatcacccg atctactacg cgggtccggc gaaaacccct gccggttatc catcaggttc
     3001 acttggccca accaccgcag gtcgtatgga ctcctatgtg gatctgctgc aatcccacgg
     3061 cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag caggttaccg acgcgtgtca
     3121 taaacacggc ggcttctacc tcggtagcat cggcggtccg gcggcggtac tggcgcagca
     3181 gagcatcaag catctggaat gcgtcgctta tccggagctg ggtatggaag ctatctggaa
     3241 aatcgaagta gaagatttcc cggcgtttat cctggtcgat gacaaaggta acgacttctt
     3301 ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag taacctcttc ggcccagcgc
     3361 ctggcagcat gctgccaggt gatccccctg ggccacctct tttgcgattg taatttcatg
     3421 cttgctggtg aatagtcagt attttccctg acttgcgaac tcaccatgac ccgcacactc
     3481 aagccgttaa ttcttaacac tggcgcactg gcgctaacgt taatcctgat ttataccggc
     3541 atttcggccc atgacaaact cacctggctg ctggaagtga caccggtgat tatcgtcgtg
     3601 ccgctactac ttgccaccgc cagacgttac ccgttaacgc tgctgctcta tacgctcatc
     3661 ttctttcacg ccatcatcct gatggtaggc gggcagtaca cctacgcgaa agtccccgtt
     3721 ggttttgagg tgcaggaatg gctggggttg agtcgtaatc cgtatgacaa gctggggcac
     3781 tttttccagg ggctggtgcc tgcactggtg gcacgagaaa ttctcgttcg cgggatgtac
     3841 gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg tggcgctggc gataagcgcg
     3901 atgtatgaat taatcgagtg gtgggcggca ctggcgatgg gccagggagc ggatgatttt
     3961 ctcggtaccc agggcgacca gtgggacacg caatccgata tgttctgcgc gctgcttggc
     4021 gcattaacga cagtgatatt gctcgctcgc tttcattgcc gccagctacg gcgctatggc
     4081 ttgataactg gataacaacg tgcactttgt tgatgccgga tgcggcgtga attccttatc
     4141 cggtctacaa aatttgcaaa tccaaagcat tacattaaga cgtggcctga taaacatatc
     4201 gcatcaggcc attttcgtta gctattgtcc acgtcaggct ttcacatcac tcactgcacc
     4261 gaccggaata tcagcatgaa caggctcggg cggtactttg cgatatttat ccagcagatg
     4321 aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag aaacggaagt agagaatcag
     4381 cgttaccatg aaaaacagag tcggtagagc aatcataata aactgcatac ctaatagggc
     4441 ttgcgtagac tgttcaacat tcggtacata gccaatcatc cctaacacaa ccgcaatgaa
     4501 aaaagccgca aaggctgaac cgcccttcac caccatagtc tgcacggagt aagcgatgct
     4561 ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg gtatctgcca ccatgatgac
     4621 ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa atacccgcaa tcacaatgag
     4681 gacgacgttg tgatagctca ttaatgccat taacaggaga acaccacagc ttaacaccgg
     4741 aagaatagat gctccggccc ataaaatgcg tcgggataat gatttaacta agcgtgggaa
     4801 gaatactaac gtcaccaggt tagcagctcc cgcatacgac agataatagg ggaacaaatc
     4861 cgcatcaccg ataacatatg agaaataata gatagcaaat ccggtaataa tgttgctggc
     4921 tacattataa gcaagagcca tacccaagag gcatgaaagc tgatcgtttt tataaataag
     4981 cgcaacgatg gctttaagtg tcagatggct tccttcagca gacgattgat tgtctgacga
     5041 aaagacttca tgcacattgc gcagagtgat gatggttgaa acaataaaaa aggcgatcaa
     5101 taccagagtg aacatctgaa agccaaatcc ccgatcgcca ccgccgacat aattaacaaa
     5161 tggtagcgtc acacctgccg taacaaagcc tgccagactg gcaaaaaaac gcggataagg
     5221 aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt ggaaccagcg accagaaggg
     5281 aatatccata atggtgtaag tcatgcccca gaggatgtag gtcacgcaaa caaagacaat
     5341 ctgagtagta ccttcaaaca gatgcgcact aaagaggaga aataagatta cagagtttgc
     5401 caacgtaccg atcaggatcc agggtttgaa cttaccccat cgcgatcgcg tagcatttac
     5461 aatccatccc ataatcggat cgttaatagc atcccagatc ctcgccacca gaaacaaagt
     5521 accgaccaaa cccacagaca gcccgacgac atcggtgtag taatacatga ggtacatata
     5581 cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat ccataactga gttttgtagt
     5641 cattgaaatg ctcatagggt atcgggtcgc tgttaccaac gcccgccttc tgttattggt
     5701 atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag tagcgtttag tcgcgtttta
     5761 acggtgcaac cagcttggca gccagtcgcc gtgggcggca atcaggtcgt caacaagagc
     5821 atatatttcg tcaatgccca gcacggcggc agtatgcggg tccatcatcg cggcgtggta
     5881 aacacggtcg cgattttccg taagaatagc ttcggtcagc agcgtctgta cgttgatgtt
     5941 ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg actttggtcg gctgaatgcc
     6001 attagcatca accagacagg ctacttccac gcaacatcct tgtggcaggt tatcaatcaa
     6061 accatcgtta cggacgttgc cgtaaatcac actcggctcg ccagtccaga tagcgttcat
     6121 gattgtgctg gcatattccc gtgacggttt aatatcaatc cgggaggcgt ttttatactc
     6181 ctccagctct ttatgccagt tcgccagctg ctcgacgcag cgtttcgggt actcatccag
     6241 cggtacttta taacgctcaa tcaaatcctc acgacctagc ttaataaacc acggcgtata
     6301 ttcggcaaaa tgttctgatg attccgtgac gaagtagccc agctttttga acatttcgta
     6361 gcgcacaata ttctggcagc gagtattacc atgaatattt ggcttcggtg cctgccctgc
     6421 ttcataagcc gccagcagtt ccgggtagag attcacgtaa ctgccgtcgg cggttttgcg
     6481 ctccagctcc aggtaaaacg ccatatggtt gatacctgcg caacggtaac gcagcgtagc
     6541 cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt ccctgcaccg aatggcacag
     6601 cccgacctgt ttgatatacg gatagcgggc atacatcgcc caggtattca tcgccattgg
     6661 gttaacatag ttgagcatgg tggcatcggg gcagacttcc gtcatgttct cgcaaatttg
     6721 ccacagatgc ggaatggtac gtagcgcgcg cataataccg cccggcccca acgtatcggc
     6781 aatggtttgt tccagaccat gccgcttaca gacctcgaaa tcagtcaccg tgcaaggttc
     6841 ataaccgcca atctgaaatg ccaccacgac aaaatcggca tcctctaagg cttctttctg
     6901 ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa tccatcagct tacgcaccac
     6961 aatatgcgac tcttccaggc gggtggggtc aatgtccatc agggcaatat gcgccgtttt
     7021 cagcgcctcg cgatggaaca catcaccaag aatattttta acgaaaatcg tcgaaccagc
     7081 gccgataaat gtaattttgg gtgcagacat catgcagatc tcctggcttg cttgaataac
     7141 ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc caggttgagc attcccataa
     7201 actcagatcc tgcgtgaagc agcagtaaat ctgagtttat gggaataaat tacctgaaaa
     7261 gtagagtttt cggcctcatg atgctcggac agtcccctcg cccctctggg aagagggtga
     7321 aaaccgtgct cccactcgca gtcatcctcc ctcactcctg ccataattct gatattctag
     7381 gaaagagagc catccatgaa tacagatacg tttatgtgca gcagcgacga aaaacagacc
     7441 cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg agattgagtt tcgcgcaccg
     7501 catatcatgc ccaccagcca ctggcatggt caggtcgaag tgaatgtgcc tttcgatggc
     7561 gatgtggaat acctgatcaa caatgaaaaa gtgaatatca atcagggtca tatcacgctg
     7621 ttctgggcct gtacaccgca ccaactaaca gataccggaa cctgtcagag catggcgatt
     7681 tttaatctgc cgatgcatct gtttctctcc tggccgctgg ataaagacct gattaaccac
     7741 gtcactcacg gcatggtgat caaatcactt gccacacagc aacttagccc gtttgaagta
     7801 cgccgctggc agcaggaatt aaacagtccg aatgagcaaa ttcgccagct cgccattgat
     7861 gaaattggcc tgatgctcaa gcgatttagc ctctctagct gggaaccgat tctggtcaat
     7921 aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg cgcaatttta tgttagccag
     7981 atgctgggct ttattgccga aaactatgat caggcgctga ccatcaacga tgtggctgag
     8041 cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc agcgggtcat gcaattgacg
     8101 atgaaacagt acattaccgc gatgcgcatc aaccacgttc gcgcgttact gagcgatacc
     8161 gataaaagta ttctcgatat tgccctgacg gcaggctttc gttcaagtag tcgtttttac
     8221 agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat accgcaaact tagccaacag
     8281 cgccgccaga cgtttcccgg ctaaagacga tatcagtatc agc
//
LOCUS       DAZXUK010000175         8321 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZXUK010000175 DAZXUK010000000
VERSION     DAZXUK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.175"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..447
                     /locus_tag="ACH3L8_003014"
     CDS             <1..447
                     /locus_tag="ACH3L8_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086032.1"
                     /translation="VDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRSDDVSPIF
                     IETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTCI
                     YKSRHKMLPINLTQETLFSSKSHGKYALFPIFTASWRATRIKNKGI"
     gene            670..1167
                     /gene="ubiC"
                     /locus_tag="ACH3L8_003015"
     CDS             670..1167
                     /gene="ubiC"
                     /locus_tag="ACH3L8_003015"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFT4086033.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1180..2052
                     /gene="ubiA"
                     /locus_tag="ACH3L8_003016"
     CDS             1180..2052
                     /gene="ubiA"
                     /locus_tag="ACH3L8_003016"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFT4086034.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2207..4630)
                     /gene="plsB"
                     /locus_tag="ACH3L8_003017"
     CDS             complement(2207..4630)
                     /gene="plsB"
                     /locus_tag="ACH3L8_003017"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFT4086035.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4801..5169
                     /gene="dgkA"
                     /locus_tag="ACH3L8_003018"
     CDS             4801..5169
                     /gene="dgkA"
                     /locus_tag="ACH3L8_003018"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFT4086036.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5279..5887
                     /gene="lexA"
                     /locus_tag="ACH3L8_003019"
     CDS             5279..5887
                     /gene="lexA"
                     /locus_tag="ACH3L8_003019"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFT4086037.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5960..7285
                     /gene="dinF"
                     /locus_tag="ACH3L8_003020"
     CDS             5960..7285
                     /gene="dinF"
                     /locus_tag="ACH3L8_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFT4086038.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7401..7610
                     /locus_tag="ACH3L8_003021"
     CDS             7401..7610
                     /locus_tag="ACH3L8_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFT4086039.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7652..8167)
                     /gene="zur"
                     /locus_tag="ACH3L8_003022"
     CDS             complement(7652..8167)
                     /gene="zur"
                     /locus_tag="ACH3L8_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFT4086040.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1897 a         2017 c         2258 g         2149 t
ORIGIN      
        1 gttgataaaa ataacaattt ttttatccaa cttatcgatc aggccttacg atcaacggat
       61 gatatgatca aagcaaatgc ctggtatctt tataaagagt ggattcgtag tgatgatgtt
      121 tcacctatat ttatagaaac tgaagaaaag ttaagaacct ttaacactaa taaattaaca
      181 cgaaacgata atatatttat cctgttctcc tctgtcgatg atgggcctgt tatggtggta
      241 agctcccagc gcttacatga tatgttgaat cctacaaaag ataccaattg gaattccacg
      301 tgtatctata aatccagaca taagatgttg cctattaatc ttactcagga aacacttttc
      361 agctccaaat ctcatggtaa atatgcgctt ttcccaattt ttactgcgag ttggcgagct
      421 actcgtataa agaataaagg tatttaagta aaggaaaaca tcaccgttcc tagcatcatg
      481 ggcggtgatg cccctatggt tgccctcgcc agcacgggca tcggcaaagc gtaaggttca
      541 acagcgtttt accacttcat gcgattgttg cgtttttgtt gcgcattaga tcacttaatt
      601 tgctttacat ctcccgtaaa cacttttctg cgatacaatg cctttacgtt atgtaacgga
      661 gagttcggca tgtcacaccc cgcgttaacg caactgcgtg cgctgcgcta ttttaaagag
      721 atccctgccc tggatccgca actgctcgac tggctgttgc tggaggattc catgacaaaa
      781 cgttttgaac agcagggaaa aacggtgagt gtgacgatga tccgcgaggg gtttgtcgag
      841 cagaatgaaa tccccgaaga actgccgctg ctgccgaaag agtctcgtta ctggttacgt
      901 gaaattttgt tatgtgccga tggtgaaccg tggcttgccg gtcgtaccgt cgttcctgta
      961 tcaacattaa gcgggccgga actagcgtta caaaaattgg gtaaaacgcc gttaggacgc
     1021 tatctgttca catcatcgac actaacccgg gattttatcg agataggccg tgatgccgga
     1081 ctgtgggggc gacgttcccg cctgcgatta agcggtaaac cgctgttgct aacagaactg
     1141 tttttaccgg cgtcaccgtt gtactaagag gaaaaaaata tggagtggag tctgacgcag
     1201 aataagctgc tggcgtttca tcgcttaatg cgtacggata agccaattgg cgcgttactg
     1261 ctgctctggc caacattatg ggcgttgtgg gtggcgacac cgggcgttcc tcagctctgg
     1321 atcatggcgg tgtttgtcgc gggtgtctgg ctgatgcgcg ctgctggttg cgtggtgaat
     1381 gattatgctg atcgcaagtt tgatggtcat gttaagcgca cggcgaaccg accacttccc
     1441 agcggcgcgg taacagagaa agaggcgcgc gcgctgtttg tagtgctggt actgatttcg
     1501 tttttactgg ttctgacgct gaatacgatg accattctgt tgtcgattgc cgctctggca
     1561 ttggcgtggg tgtacccgtt tatgaagcgg tatacccatc tcccgcaagt ggtgctgggc
     1621 gcggcgtttg gctggtcgat tccaatggct tttgccgctg tgagtgagtc ggtgccattg
     1681 agttgctggt taatgttcct cgccaatatt ctctgggcgg tggcttacga cacgcagtat
     1741 gcgatggttg accgcgatga tgatgtgaag attggcatta aatccacggc aatcctgttc
     1801 ggccaatacg ataaattgat tattggtatt ttgcagattg gcgtactggc actgatggcg
     1861 atcatcggtg agttaaatgg cttaggctgg ggatattact ggtcaattgt ggtggctggc
     1921 gcgctgtttg tttatcaaca aaaactgatt gccaaccgcg agcgtgaagc ctgctttaaa
     1981 gcatttatga ataataacta tgttggtctg gtactgtttt taggactggc aatgagttac
     2041 tggcatttct gatgatgtaa aaaagccgga tgatcatccg gctttcttct gggttgcctg
     2101 atgcgttgcg cttctcaggc ctacccggtg aatcgcaatt tattgaattt gcaggtcatg
     2161 gtaggccgga taaggcgttt tcgccgcatc cggcaattct ctctgattac ccttcgccct
     2221 gcgtcgcact ctcaatcgtc aaacgcacgt ctgatgtaat caactccgcc agcaactgat
     2281 aaaccttcat cgtttctgct ggttcggcat cgccgctatc gctgatatac ccttcatcac
     2341 gcagcgtcag caccagagaa ctgaacaccg ccttgtcgaa gaactccggc gcgttgatgc
     2401 cgtgcagcac ggagagacgt tgcgcaacgg tgcggctctc tttctccagc gtaccgcggt
     2461 tgatcgacgg gttggcactc aacaaccaga aggtgatggc ataacgttgc agcgtttcgc
     2521 gcgcgcctgc ggccagcagc tgtagggtgc gagaatgcgc cgggttgata tgcaactcat
     2581 catcttgcag ggtaatcagc ccctgacgtt gcatctcatt tgccagcgca tcaataacgt
     2641 ccggcaactc gtcgcgatcc cagcgcagga acagctccgc tttcagcatt gggtaaagca
     2701 cattgacgtg ctccatcaat acgtcgcggg agatgtggcg atgctgggtg acaattgccg
     2761 ccatcagcga aggcagcacc aacatatgcg caatgttgtt gcgatagtag gtcatcagca
     2821 ccgcttgctc gcgtggcaga atgatgatgt cgccgatagt gtctttctcg acttcaaact
     2881 tgttcatttg cagcgcgtga tcgataagct cgctggcgct ggctgaagga acggtagagt
     2941 ccgtggagta aggtacgttg cgcatcagat ccaggtagca gttgagttgc tcggttaact
     3001 gctcgcgggt gagtgagcgc tgacgtgatg ccagtagcgc agtacagcac aggttcatgg
     3061 cgtttgccgc gcctgcgttg ttaatgcgta ccatcagatc ggcagcaata ttattgaccg
     3121 tcggcgttaa ccaggccgga cgcaccgctt cgatgggatc gatagattca cgccagtctg
     3181 gtacgtgctg gttaaggtag gtcatcaacg gcattggttc accgaagttg acgtaaccct
     3241 gaccgagatt acgcagcttg cttaaaccgc gcagcatctg cggcaggctc tctttctctt
     3301 tcgtcgcgcc gcgcagttct ttggcgtaag tacccacttc catgacgtgc tcataaccga
     3361 tatagatcgg aatcagcgta atcggacgcg tgccgccacg cagcatcgcc tgaatggtca
     3421 tcgacagcgt accggttttc ggatccagca aacgccccgt gcgggaacga ccgccttcca
     3481 cgaagtactc gacggaataa ccacggctga acagttcgcc gagatactca cggaaaacgg
     3541 tggaataaag tttattgcct ttaaaagtac ggcgaataaa gaacgccccc agacggcgga
     3601 aaatcggccc ggccggccag aagttcaggt tgatcccggc ggcgatatgc ggcggcacca
     3661 gcccctggtg gtacagcacg taagaaagta gcaggtagtc catgtgactg cggtggcaag
     3721 gcacatacac cagctcatga ccgtcgtggg ccagctggcg aacgcgctcg gcgttatgga
     3781 cgttgatgcc ctgataaaat cggttccagg tgaagcccag aatgcggtca gtcaggcgga
     3841 tcatctcgta agagaaattc gccgcaatct cttccatcag cgcaatcgcg ttctgctgcg
     3901 ctttttcatg ggagattttt ttgctgcgcg cttcatcttc taccgctttg gcaatggcgc
     3961 gggaggcgag cagcttatta aacagatcct gacgagcagg cagacgtggg cctacggcag
     4021 ccagacgttg acgggcaaag tgcatacgcg ccacgcgcgc cagtttctga gcgatagttt
     4081 tatccgtgcc gtgttcatcc gccatacggc gcagcgacac tgacggcgag aaacgcacaa
     4141 aactgtcgcg accgagccac agtacagcga aaaatttctg tacgccgtta agcatacgca
     4201 gcggcgggtt cacttcgcct ttttcacgcc ccggcgcgcg accaaacatc accgatactg
     4261 gcaccatctg cacatccaga tttgggttgc tacggtgcaa atcgagatag tcgtggaaaa
     4321 gcttaataga ctcttctttc ggcgtgtaat aggtgaatac acgcggcccg ccgtgaatga
     4381 acacatagcg cggcagtagc gtgccgtcga tttccagcgg ctctaacggg tcaggcaaat
     4441 catgtgccag acactgggcg cgcaacgtca gcaaatctgc tttcgagttg tacggtaaaa
     4501 cgtacataat tggacgagag gtatccagcc ccagttccgg ggcaggatct gccggaatag
     4561 acttgctttt taccaggatg cttaatggta aattcagtaa tttgtagtaa attcgtggcc
     4621 agccggacat aaacgatgta aagcctctgg ttaataatgc aaatgcgcgg caaggatagc
     4681 agaaagtcat gggaaattct gtggtatccg ctcatgtttc gcgcggcgtt acgcaaaccc
     4741 gaatcatcgg atttaacggt acactgatat tgacgctcat aatgtaaaaa ggttctttca
     4801 atggccaata atacaactgg attcacccga attatcaaag ctgctggcta ttcctggaaa
     4861 ggtttacgcg ctgcatggat caacgaagcg gcattccgtc aggaaggcgt agcggtattg
     4921 ttggcggtgg tcatcgcctg ctggctggat gtggacgcaa ttacccgcgt gctgcttatc
     4981 agctccgtga tgctggtgat gattgtggaa atcctcaata gcgccatcga agcagtggtt
     5041 gaccgaattg gctctgaata ccatgagctt tccggacgcg caaaagatat gggatccgcc
     5101 gcggtgctga ttgccattat cgtcgccgtg attacctggt gcatcctgtt atggtcgcat
     5161 tttggataac ccttccagat ttcgataaat ctctggttta ttgtgcagtt tatggttcca
     5221 aaatcgcctt ttgctgtata tactcacagt ataactgtat atacacccag ggggcggaat
     5281 gaaagcgtta acggccaggc aacaagaggt gtttgatctc atccgtgatc acatcagcca
     5341 gacaggtatg ccgccgacgc gtgcggaaat cgcgcagcgt ttggggttcc gttccccaaa
     5401 cgcggctgaa gaacatctga aggcgctggc acgcaaaggc gttattgaaa ttgtttccgg
     5461 cgcatcacgc gggattcgtc tgttgcagga agaggaagaa gggttgccgc tggtaggtcg
     5521 tgtggctgcc ggtgaaccgc ttctggcgca acagcatatt gaaggtcatt atcaggtcga
     5581 tccttccttg ttcaagccga atgctgattt cctgctgcgc gtcagcggga tgtcgatgaa
     5641 agatatcggc attatggatg gcgacttgct ggcagtgcat aaaactcagg atgtacgtaa
     5701 cggtcaggtc gttgtcgcac gtattgatga cgaagttacc gttaagcgcc tgaaaaaaca
     5761 gggcaataaa gtcgaactgt tgccagaaaa tagcgagttt aaaccaattg tcgttgacct
     5821 tcgtcagcag agcttcacca ttgaagggct ggcggttggg gttattcgca acggcgactg
     5881 gctgtaacat atctctgaga ccgcgatgcc gcttggcgtc gcggtttgtt tttcatctct
     5941 cttcatcagg cttgtctgca tggcattcct cacttcatct gataaagcac tctggcatct
     6001 cgccttaccc atgattttct ccaatatcac cgttccgttg ctgggactgg tcgatacggc
     6061 ggtaattggt catcttgata gtccggttta tttgggcggc gtggcggttg gtgcaacggc
     6121 gaccagcttt ctctttatgc tgttgctgtt tttacgcatg agcaccaccg ggctgactgc
     6181 gcaggcttat ggtgccaaaa atcctcaggc attagcccgt gcgctggtgc aaccgttgct
     6241 gttggcgttg ggggccgggg cgttaattgc gctgttgcgt acgccgatta tcgatctggc
     6301 gctgcatatt gttggcggta gtgaagcggt cctggaacag gcgcggcgct ttcttgaaat
     6361 ccgctggtta agcgctccgg cgtcgctggc gaatctggta ttactcggtt ggttactcgg
     6421 cgtgcaatat gcccgtgcgc cagtaatttt gttagtggtc ggcaatatcc tcaacattgt
     6481 gctggatgtc tggctggtga tggggctgca tatgaacgtg cagggcgcgg cgctggcgac
     6541 ggttattgcg gaatatgcaa cattgctgat tggtctgcta atggtgcgta aaatcctcaa
     6601 actacgcgga atttccggcg aaatgctgaa aactgcctgg cgaggaaact tccgtcgctt
     6661 gctggcgctt aaccgcgata tcatgctgcg ttcgctgttg ttgcaactct gtttcggcgc
     6721 gatcaccgta cttggtgcgc gactggggag tgacattatc gctgttaacg cggttctgat
     6781 gacgctactc acctttaccg cctatgcgct tgatggtttt gcctacgcgg ttgaagcgca
     6841 ctccggtcag gcatacggtg cgcgcgacgg tagccagttg ctggatgtat ggcgggcagc
     6901 gtgccgccag tcggggattg tagcgttact attttcggtg gtttatttgc tggcagggga
     6961 acacatcatt gcgttgctga cgtcgttaac ccagattcag cagctggctg accgctatct
     7021 tatctggcag gtgattttgc cgttggttgg cgtctggtgt tatctgctgg acggcatgtt
     7081 tataggcgca acgcgtgccg ccgaaatgcg taacagtatg gcggtggccg ccgcaggttt
     7141 tgcgctgacg ctccttacgc tgccgtggct gggtaatcat ggtttgtggc tggcattaac
     7201 cgtctttctg gcgttgcgcg ggctttctct ggcggctatc tggcggcgtc actggcgcaa
     7261 cggtacctgg tttgccgcaa cgtgacggtt aaaaattctg aataaattat cctaagccaa
     7321 attgctgact acacttaatc tcacgttcag aagaaaagtg aacgtactct cattcacaac
     7381 ctaacgatga ggtcttgatt atgaataaag atgaagccgg cggtaactgg aaacagttta
     7441 aaggtaaagt gaaagagcaa tggggcaaac tgaccgatga tgatatgacg atcattgaag
     7501 gtaaacgtga tcaactggtc ggtaaaatcc aggaacgtta tggttatcag aaagatcagg
     7561 cagaaaaaga ggtcgtggat tgggaaaccc gcaatgaata tcgctggtaa ttaatccctc
     7621 ctgcccgacg tgtacaagga tgtacgccct cttaacgcgg tttctttttc acctgcacag
     7681 agtgatcatg ctggcactgt tcaggatgac gacacgcttc cacttccacg catgccgcac
     7741 agagcccatg tgcttcaatc acattatgcc gcagggcaaa ccccattttt gccgccagcg
     7801 tatgcataat gtcttccacg ccttctgcac actcttcttt caccgcgccg cagcgatcgc
     7861 aaataaacat ggctgacgta tgcgtgggct ggtcgaacag atgacagagc acataactgt
     7921 tggtggattc caccttatgc acaaaacctt gctcaagcag aaaatccagc gcgcgataaa
     7981 ccgttggcgg cttggcttgc ggttcagctt cgcgcagtaa atcaagcaga tcataagcgc
     8041 tgatagcgcc atcctgcaga ctcatcaggc gcaacacttc caggcgctgt ggggtcaggc
     8101 gcacattacg ctgcgcgcag attttttcag cctgcgctaa taactcctgc gttgtggtct
     8161 tttccatcgg gcacctcaaa gtgggggtta agaatgtcct tactttacca tgttccagga
     8221 aaaacaccga gaacccttat tgttgccgta atgttgattt tctgttttgt aggtaaggtg
     8281 ttgtgttgcc ttatcgtacc attatcaaca cgataataat t
//
LOCUS       DAZXUK010000176         8288 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZXUK010000176 DAZXUK010000000
VERSION     DAZXUK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.176"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(380..1576)
                     /gene="hemY"
                     /locus_tag="ACH3L8_003023"
     CDS             complement(380..1576)
                     /gene="hemY"
                     /locus_tag="ACH3L8_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFT4086041.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1579..2826)
                     /gene="hemX"
                     /locus_tag="ACH3L8_003024"
     CDS             complement(1579..2826)
                     /gene="hemX"
                     /locus_tag="ACH3L8_003024"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFT4086042.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPAPQGDTPAAAPQGE"
     gene            complement(2848..3588)
                     /gene="hemD"
                     /locus_tag="ACH3L8_003025"
     CDS             complement(2848..3588)
                     /gene="hemD"
                     /locus_tag="ACH3L8_003025"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFT4086043.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3585..4526)
                     /gene="hemC"
                     /locus_tag="ACH3L8_003026"
     CDS             complement(3585..4526)
                     /gene="hemC"
                     /locus_tag="ACH3L8_003026"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFT4086044.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4913..7459
                     /gene="cyaA"
                     /locus_tag="ACH3L8_003027"
     CDS             4913..7459
                     /gene="cyaA"
                     /locus_tag="ACH3L8_003027"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFT4086045.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7499..7819)
                     /gene="cyaY"
                     /locus_tag="ACH3L8_003028"
     CDS             complement(7499..7819)
                     /gene="cyaY"
                     /locus_tag="ACH3L8_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFT4086046.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7867..>8288)
                     /locus_tag="ACH3L8_003029"
     CDS             complement(7867..>8288)
                     /locus_tag="ACH3L8_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFT4086047.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1846 a         2183 c         2157 g         2102 t
ORIGIN      
        1 gatcaagctc aggttaaata ttcactcagg aagttattac tcaggaagca aagaggatta
       61 cagaattatc tcataacaag tgttaaggga tgttatttcc cgattctctg tggcataata
      121 aacgagtaga tgctcattcc atctcttatg ttcgccttag tgcctcataa actccggaat
      181 gacgcagagc cgtttacggt gcttatcgtc cactgacaga tgtcgcttat gcctcatcag
      241 acaccatgga cacaacgttg agtgaagcac ccacttgttg tcatacagac ctgttttaac
      301 gcctgctccg taataagagc aggcgttttt ttatgcatca gaaaggtccc ggaggtgctt
      361 tcctccgggt gagaaggaac tactgtggcg ggttattctg caacgttaac atcaaaccgt
      421 cgcgacgcat cgctgcagct tcttccggct tgtgcagtct gtccagcgcg tcggcaagcc
      481 atgcgtaatc gtaggcgtcc ggacgttgtt tcagcgccgc gcggaaggcg agcgatgctt
      541 cctgccattc accgtgcttc atcagtgact gacccaatgt gctccacaac agcgggcgat
      601 caccgacgtt tttgatttgc tggcgtagca ctttttccag ctgttccggg ttattggttt
      661 tcagacgcgg gatcggcagc agtaggcgat cgtcatactg gcgtttcagg ccgtcgatga
      721 taatttgctg ggcagtatca tgatcgtcac attcaataag atgttccgcc attgccacct
      781 gcaacgctac ctgatgacgc gttttccggc tttggttttt ccaccagtta cgcaaacctt
      841 cactaccgtt atcggcacgc gcctgatcca tcaggccaat ccatgcctgt tgttccagca
      901 ttgcacgatg ttcttcatca ccaacatggg ctttcgccat tgatgggatg atatccagca
      961 gagaactcca tgcccctgtg cggatatacg cctgttccgc cagacgtaat acttcaggat
     1021 ggcgtggcgt aacttccagc agcttatcca cgccgtggcg tgcagcatgg ttttcattac
     1081 gggccagttg cagacgtaca cgggtgattt ctaccggaat ggtgtcattg ccggccagct
     1141 ccgctgcgcg ttccagatgt tggttggcgc gtgcttcatc accacgttgt tgcgcggctt
     1201 cggcagccag tagatagttc accaccggtt gttcagcgtg atcggcattt ttcgccatca
     1261 gcttttcaac ttgctgataa tcaccttccg ccagtttcag cagcgcctgt tcggtctgct
     1321 tacgcgcacg gcgacgctta cgtccgacaa accatccacg ggtgtgcgcg ccagtgcgga
     1381 agatccgccg cagtagccac tcaatggcaa acagcactac catcgccaga atcaatatga
     1441 tcgccaggcc cgtgacgctg gtttcgatat tgtagttgtc ggtctggatc agcacataac
     1501 cctgatgacc ggcaatcatc gggccaacca ctatccccgc aatcagcaaa acaaagagta
     1561 ataacacttt tagcatggtt attctccttg cggcgcggct gccggagtat caccttgcgg
     1621 tgcaggtgca ggtgcaggtg caggtgcagg tgcaggtgca ggtgcaggtg caggtgcagg
     1681 tgcaggtgca ggtgcaggtt tagcttccgt tgtccccgct gccggttgtg ccagcaggtt
     1741 acgcacgcga gtctgcatca gtttttccag catcgcctgg ctttgcaggg tttccggaag
     1801 atccatcgag atattttgct ggcttaactg gtccacctcg tcgaggaacg ctttggtggt
     1861 ggcatcatca gtatcgtagt aagcacgtac ccaggtggag acgttctcca gcgcctggcg
     1921 ataagtctct tcctgatgac gcggtacagc ttgtgctgcg accagcaggc gagagcgaat
     1981 attttcgcgc agatagatat cctgatttgg cgctaacagc ggtacggcgg tgtcatcacg
     2041 acggcgaatc gtaatgaagt tgtccataaa gttctgccag cttttttgca gattgatacg
     2101 ccattcgctg atggaactgg aaagctcttc accgtctgaa tccatcggcg aaccatcgct
     2161 gtcattatcg gccagacgca ggttatctac ctgatttgaa agctgattaa gcttaaggat
     2221 gatgccgtca taatccacct gcgatactgc agaaaggctg gcgatatcat cggtaattgc
     2281 ccgacgaacg gtaatcagac tcgggtcatt catatccgcc aggctggcgt ctgcactttt
     2341 cagcaacgct gcagaggtcg tgacgtcctg atcgctccac agcttccgtc cggcgagttt
     2401 caccagaaaa tcggcctgag ccagcagcca ggttttagca tcgctgccgg aaatggtggc
     2461 gatcttttgt tggacttcat ccaactgttt tgccagcgtt tcttgctgac gattcgcctg
     2521 cttaagctgc gcagcttgtt gcttaataat gccttccagc tcggcttttt ggctctcctg
     2581 ggctttttgc aatgccgtca gttggttagc cagggcatcg ctggtggcgg tctgattgac
     2641 ggcctgttgt ttaccccagc catacaaacc gacacccgcc gccagagcaa tagcgatagc
     2701 caccgcgctg agaatcaatg cagtattgtt cttactcttt ttttctgttg cgacaggttg
     2761 tgacgtggtg tccacggcct ccctggtctc ttcaaccacg gcggaggttt tttcttgttc
     2821 cgtcattatg gcttcctgtt atgagagtta ttgtaatgcc cgtaaaagcg catcgttgtc
     2881 agcgttatcg gcgaccttaa tgtcttgcca gcccagttcc cgggcgagtt tcgccaaacg
     2941 ctcactgacg accaatagtc gacagtgtag taaccagtgc tcacgatacc attgtgggat
     3001 cagcgaccag agttgctgca acatttcacc gctggtaaca acgaccatcg tcacctcgcg
     3061 ggattgccag cgcatcgctt cttctgcacc atcgtaatgg attgcgcatc gttgataaca
     3121 ttcacaaaaa gtgacctcag caccgcgcac cgtcagggta tccccaatta gctcacgacc
     3181 gccattgcca cgtaatatca gcgcacgttt gcccgcaata ttttgtaatt caggtaattg
     3241 tagcaagact tcgctgattt cccgatcctg tgggtagaga atcttctgtc cgcttacggt
     3301 atgtagcgcc agtgcggtgg tgcgtccaat ggcgaaataa gtaggtagtt ggggccattt
     3361 acgatcttgc tgatgcagct gtgattgggc aaaagcaacc gcgtgttgcg agagggcaaa
     3421 caacagatcg ctctccccca gcgctgccag ttgatcagca agttgcggta attgtcgacc
     3481 cggagaaaac tcaatcagcg gaaaatgcca ggccacctgc cccagtgtgc gcagacggct
     3541 cactaactct tctccagcgg gagacgggcg ggtgaccagg atactcatgc cggggcctct
     3601 ccgttataga cttcagcgag gatctcgcgc gcgccgttat tcagtagctc ttctgccagc
     3661 gaaatcccca tttgttcggc atcttgcggc gcaccgcggc gttcaccgcg aataatctgc
     3721 gaaccgtccg gcgcgccgac cagcgcacat agccagattt cgccatcaat aagctcggcg
     3781 tagctaccaa ttggcacctg acatccgcct tcgagacggg tattcatggc gcgttctgcg
     3841 gtaacgcgca gtgcagtttc gtggtgattc agcgcggcaa gcagctcgcg agtgcgtgta
     3901 tcatcaaggc ggcattcaat accgactgca ccttgtccta ccgccggaag agaaatctcg
     3961 ggtggtaacg cggcgcgaat gcgggactcc agacctaaac gttttagtcc ggctacggca
     4021 agaatgatgg catcgtattc gccgttatct agtttgctca ggcgagtgcc gacgttgccg
     4081 cgcagggagc ggataatcag atccgggcgg cgttcagcca gttggcactg gcgacgtaaa
     4141 ctggacgtcc cgacgatact gcctgctggt aacgcatcca gactgtcata gttattggac
     4201 acaaaggcat cgcgaggatc ttcacgctcg caaatagtga ccagccccag accttgcggg
     4261 aattcaaccg gcacatcttt cattgaatgt acggcgatat cagcgcgatt ctccaggagc
     4321 gcgacttcca gctcttttac aaataagcct tttccgccta ctttcgccag cggcgtatca
     4381 agaatcacat cgccgcgcgt caccatcggt accagttcaa cgaccaggcc cggatggctc
     4441 gccatcaact tgtctttgac atagtgtgcc tgccagagtg caagtgggct ttggcgtgtg
     4501 gcaattctta aaacattgtc taacatgctt gttaccgtca ttatcatccg tggtccatcc
     4561 taacatcctt gccagagtga tgtcagtgtt gtggtgaaac gtagacgcct gcgcaaaccg
     4621 taaaatgagg tctggcagtg gatcctgaca ggcgtttcac gccgtcgtaa taaggaattt
     4681 acagagaata aacggtgcta cacttgtatg tagcgcatct ttctttacgg tcaatcagca
     4741 aggtgttaaa ttgatcacgt tttagaccat tttttcgtcg tgaaactaaa aaaaccaggc
     4801 gcgaaaagtg gtaacggtta cctttgacat acgaaatatc ccgaatgccg cgtgttaccg
     4861 ttgatgttgg cggaatcaca gtcatgacgg gtagcaaatc aggcgatacg tcttgtacct
     4921 ctatattgag actctgaaac agagactgga tgccataaat caattgcgtg tggatcgcgc
     4981 gcttgctgct atggggcctg cattccaaca ggtctacagt ctactgccga cattgttgca
     5041 ctatcaccat ccgcgaatgc cgggttacct tgatggtaac gttcccaaag gcatttgcct
     5101 ttacacgcct gatgaaactc aacgccacta cctgaacgag cttgaactgt atcgtggaat
     5161 gtcagtacag gatccaccga aaggtgagct tccaattact ggtgtataca ccatgggcag
     5221 cacctcgtcc gtagggcaaa gttgttcctc tgacctggat atctgggtct gtcatcaatc
     5281 ctggctcgat agcgaagagc gccagttact acaacgtaaa tgcagcctgc tggaaagctg
     5341 ggccgcctcg ctgggtgtgg aagtcagctt cttcctgatt gatgaaaacc gcttccgtca
     5401 taatgaaagc ggcagcctgg ggggcgaaga ttgtggctcc acccagcata tactgctgct
     5461 tgacgaattt tatcgtactg ccgtgcgtct cgccggtaag cgtattctgt ggaatatggt
     5521 gccgtgcgac gaagaagagc attacgacga ctatgtgatg acgctttacg cgcagggcgt
     5581 gctgacgcca aatgaatggc tggatctcgg tggcttaagc tcgctttctg ctgaagagta
     5641 ctttggtgcc agcctttggc agctctacaa gagtatcgat tccccataca aagcggtgct
     5701 gaaaacactg ctgctggaag cctattcctg ggaatacccg aacccacgtc tgctggcgaa
     5761 agatatcaaa cagcgtttgc acgacggcga gattgtatcg tttggcctcg atccatactg
     5821 catgatgctg gagcgtgtta ctgaatacct gacggcgatt gaagatttca cccgtctgga
     5881 tttagtacgt cgctgcttct atttaaaagt gtgcgaaaaa ctcagccgtg aacgcgcctg
     5941 tgtaggctgg cgtcgcgcag tgttgagcca gttagtgagc gagtggggtt gggacgaagc
     6001 tcgtctggca atgctcgata accgtgctaa ctggaagatt gatcaggtgc gtgaggcgca
     6061 caacgagctg ctcgacgcga tgatgcagag ctaccgtaat ctgatccgct ttgcgcgtcg
     6121 caataacctt agcgtctccg ccagtccgca ggatatcggc gtactgacgc gtaaactgta
     6181 tgccgcgttt gaagcattgc caggtaaagt gacgctggta aacccgcaga tttcacccga
     6241 tctctcggaa ccgaatctga cctttattta tgtgccgccg ggccgggcta accgttcagg
     6301 ttggtatctg tataaccgcg cgccaaatat tgagtcgatc atcagccatc agccgctgga
     6361 atataaccgt tacctgaata aactggtggc gtgggcatgg tttaacggcc tgctgacctc
     6421 gcgcacccgt ttgtatatta aaggtaacgg cattgtcgat ttgcctaagt tgcaggagat
     6481 ggtcgccgac gtgtcgcacc atttcccgct gcgcttacct gcaccgacac cgaaggcgct
     6541 ctacagcccg tgtgagattc gccatctggc gattatcgtt aacctggaat atgacccgac
     6601 agctgcgttc cgcaatcagg tggtgcattt tgatttccgt aagctggatg tcttcagctt
     6661 tggcgagaat caaaattgcc tggtaggtag cgttgacctg ctgtaccgca actcgtggaa
     6721 cgaagtgcgt acgctgcact tcaacggcga gcaatcgatg atcgaagccc tgaaaactat
     6781 tctcggcaaa atgcatcagg acgccgcacc gccagatagc gtggaagtct tctgttatag
     6841 ccagcatctg cgcggcttaa tccgtactcg cgtgcagcaa ctggtttctg agtgtattga
     6901 actgcgcctt tccagcaccc gccaggaaac cgggcgtttc aaggcgctgc gcgtttctgg
     6961 tcaaacctgg ggcttgttct tcgaacgcct gaatgtatcg gtacagaagc tggagaacgc
     7021 catcgagttt tatggcgcta tttcgcataa caaactgcac ggcttgtcag tgcaggttga
     7081 aaccaatcac gtcaaattac cggcggtggt ggacggcttt gccagcgaag ggatcatcca
     7141 gttctttttc gaagaaacgc aagacgagaa tggctttaat atctacattc tcgacgaaag
     7201 caaccgggtt gaggtgtatc accactgcga aggcagcaaa gaggagctgg tacgtgacgt
     7261 cagtcgcttc tactcgtcat cgcacgaccg cttcacctac ggctcaagct tcatcaactt
     7321 caacctgccg cagttctatc agattgtgaa ggttgatggt cgcgaacagg tgattccgtt
     7381 ccgaacaaaa tcgattggca acctgccgcc cgccaatcag gatcacgata cgccgctatt
     7441 acagcagtat ttttcgtgat gagcgtgccg gaaagcgagg cttatccggc atgcaatctt
     7501 agcggaaact gactgtttca cccgcctgtt gcgtcgccgc ctgttccagc aaatcccaga
     7561 aggtttcgcc gctgcgatcg caaatccatt catcgccttt caggtcaaaa tggtagccgc
     7621 cctgtttggt tgccagccac acctggtgca gcggctcctg gcggttaata atgattttgc
     7681 tgccgttttc aaaggtaatg gtcagtacgc cgccgttgat ttcgcagtcg atatcgctgt
     7741 cgccatccct gtcgtccaga cgctcttcaa tggtcagcca cagttgatca gccaggcgat
     7801 gaaattcact gtcgttcatt gttgtatcct gtttttaagt gatggcggca gtatagcggc
     7861 atggggtcag ggcttcaaag tttgcacctc tgctgctgcg ttccggcatg attcatccgt
     7921 caccggaata atgatgtctc tgttttgaac ttcacgtacg ttctcctctc tgacacgagt
     7981 tttcgccggg cagggggagg gcgtaatcct tacctctgta tcggtccatg tataaacatt
     8041 gcgatcgtca taagcgaagc gatcgttttt agttagcgaa ccgttaactg gaataatttg
     8101 caagctcttt gaccgtaatt ggcggataaa gtaagcatgc tgtttgccgc tattaaatga
     8161 gcgatcaatg accaggtctg gcgtatccag cgggattatc tcaagctggt cttcaccaaa
     8221 tgtcgataat tttgttacat acagccgatc tttatattgc gcaatattgc cagtaatcag
     8281 atggaatt
//
LOCUS       DAZXUK010000177         8257 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZXUK010000177 DAZXUK010000000
VERSION     DAZXUK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8257
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.177"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            90..344
                     /gene="yjbL"
                     /locus_tag="ACH3L8_003030"
     CDS             90..344
                     /gene="yjbL"
                     /locus_tag="ACH3L8_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFT4086048.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            335..1075
                     /locus_tag="ACH3L8_003031"
     CDS             335..1075
                     /locus_tag="ACH3L8_003031"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFT4086049.1"
                     /translation="MFLIEHINDLKMWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFA
                     INSICNPGDNKDAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGF
                     LTFVNACQEHDDNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQR
                     DMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSF
                     DPTSKEEIKILTDTSKLIYDLFGVKSEK"
     gene            1438..2475
                     /gene="dusA"
                     /locus_tag="ACH3L8_003032"
     CDS             1438..2475
                     /gene="dusA"
                     /locus_tag="ACH3L8_003032"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFT4086050.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2609..2851
                     /gene="pspG"
                     /locus_tag="ACH3L8_003033"
     CDS             2609..2851
                     /gene="pspG"
                     /locus_tag="ACH3L8_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFT4086051.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(3017..4000)
                     /locus_tag="ACH3L8_003034"
     CDS             complement(3017..4000)
                     /locus_tag="ACH3L8_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFT4086052.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            4083..5498
                     /gene="dnaB"
                     /locus_tag="ACH3L8_003035"
     CDS             4083..5498
                     /gene="dnaB"
                     /locus_tag="ACH3L8_003035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFT4086053.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5551..6630
                     /gene="alr"
                     /locus_tag="ACH3L8_003036"
     CDS             5551..6630
                     /gene="alr"
                     /locus_tag="ACH3L8_003036"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFT4086054.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6883..8076
                     /gene="tyrB"
                     /locus_tag="ACH3L8_003037"
     CDS             6883..8076
                     /gene="tyrB"
                     /locus_tag="ACH3L8_003037"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFT4086055.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         2072 a         1911 c         2120 g         2154 t
ORIGIN      
        1 gttcagaaat tatgagcgat ttttgatagt gaccagatgg caaaagaatt ataaggagga
       61 cacggtacga caaaaaaggt agatgaatca tgttgaagat aatacccggt gcaactggat
      121 actttaataa aacactcaat tcaaatcaat tcgaaaataa agatgcaacc aaagataaat
      181 tggataatag aggttcaatc aaaggtaaat taaataatat atatggtaaa tctattgatt
      241 atgcagcgct gcgtcatcgg gatataatta ttgcaaaaat agatttgttt attcaacgaa
      301 tcacgcataa tttatggcat gcacgtaaaa aaatgtgttt ttaattgagc acataaatga
      361 tctcaaaatg tgggtcaata agtatactga cgattgcact gatgaggatt taaacgatcg
      421 tgactttatt gcatcagttg ttgaccgggc tatttttcat ttcgcgatta atagtatatg
      481 taatcctggg gataataaag atgcaatgcc cattgaacaa tgtacttttg atgtagaaac
      541 taagaatggc cttccctcca cggttcagct attttatgag gaatctaagg ataatgaacc
      601 tttagcgaat atacattttc aagcaatagg ttctggtttt ttaacgtttg ttaatgcctg
      661 ccaggaacat gatgacaaca gcttaaaatt atttgcttcg ctgttaattt cactttcata
      721 ttctagtgcc tacgcagatt tatcagaaac agtgtatatt aatgaaaata atgagagcta
      781 cctgaaagct cagtttgaaa aattatatca acgtgatatg aagaagtacc tgggagagat
      841 gaagcgtctg gcagatgggg gagaaatgaa ttttgatggc tatctggata agatgtcaca
      901 tctggtgaat gaaggaacgc tcgatcctga tattttaagc aaaatgcgag atgctgcacc
      961 acaattaatt agcttcgcga agtcgtttga cccaacctca aaggaagaga ttaaaatact
     1021 tacagacact tctaaattaa tttatgactt gttcggggtt aaatcggaga aataatatgt
     1081 gaagttcttc gatagtatgg aaggcattat ataaaaggac ccaatattta ttgggttctt
     1141 ttttctctat caatgctatt agcagggaga tatatcacca gagtttaatg tgtgattttt
     1201 tatttatcgt cgaacctgga ttgtttatca ttggccttaa caaagttaac ggctaataag
     1261 attatttcca tcacttcgtg agagcttcat gccttgagag gatctcaatt ttcttttgca
     1321 atgagacagg cgcttcctgt tgttatggta tagtaccccg ctattgagcc tcctgaacag
     1381 tgaagctgaa taacataacc ccatgatata tcgataaaat aatctctaca tttgaaaatg
     1441 cacggtaatt ctgaaatgca aaaaatcaac caaaccagcg caatgcctga aaaaactgac
     1501 gttcactgga gtggtcggtt tagtgttgca ccaatgctcg actggacgga cagacattgc
     1561 cgctatttct tgcgtctgct ttcccgcaat acgttgcttt ataccgaaat ggtgaccaca
     1621 ggggcgataa ttcacggtaa aggtgattat ctggcgtaca gtgaagaaga acatccggta
     1681 gcgttgcaac tgggcggtag cgatccggcg gcgctggcgc agtgtgcaaa gctggcagaa
     1741 gcgcgcggat atgatgagat caacctgaat gtcggctgcc cgtctgaccg ggtgcagaac
     1801 ggcatgtttg gtgcgtgtct gatgggtaat gcgcagctgg ttgccgactg cgtgaaagcg
     1861 atgcgcgatg tggtgtcgat tccggtgacg gtgaaaacgc gtattggcat cgacgaccag
     1921 gacagctatg aatttctctg cgatttcatc aataccgttt ccggcaaagg cgagtgtgag
     1981 atgttcatca tccacgcacg taaagcctgg ctttcggggt taagcccgaa agaaaaccgt
     2041 gaaatcccgc cgctcgatta tccgcgtgtg tatcaactga agcgtgactt cccgcatctg
     2101 acaatgtcga ttaacggtgg tatcaagtcg ctggaagaag ctaaagcgca tttgcaacat
     2161 atggatggcg tgatggtcgg gcgcgaggcg tatcagaatc cgggtattct ggcggcggta
     2221 gaccgggaga tctttggttc ctcggatacc gatgccgatc cggtggcagt agtgcgtgcc
     2281 atgtatccgt acattgagca tgaactcagc caggggacgt atctcggcca tattatccgg
     2341 catatgctgg gtttgttcca gggtattcct ggcgcgcggc agtggcggcg ttatttaagt
     2401 gaaaatgccc ataaagcggg tgctgacatt aacgtgctgg aacacgcgct caaactggtg
     2461 gcggataagc gttaactttt caccaaaaag tagtcaaatt caccacgccc tgcgcaccgt
     2521 cgcggggcgt tttgctgtta aatcaataga ttatttttgg catgattctt gtaatgccag
     2581 caagagattt catatttggg agagcatcat gctggaacta ctttttgtga ttggcttttt
     2641 tgtcatgctg atggtcaccg gcgtttcgtt gctgggcatt atcgccgcgc tggttgtggc
     2701 gacggccatt atgttcctcg gcggtatgct ggcattgatg attaagttgc tgccgtggtt
     2761 actactggcg attgcggtgg tgtgggttat taaggcgatt aaagcaccaa aaatgccgaa
     2821 atatcagcgt tatgaccgct ggcgttacta agggattgtg cggatgatca caacctaagg
     2881 ttttatcctt agaacaaaat aggaattgat aatcaaatct gtcactattg cgcctctaac
     2941 agattcatcg tgctgtaccc tacatacagc cgaactataa aaagaaaggg cttcccaggt
     3001 ggaagcccta tttcttttat ggaatcagca ggctggaacc ttgcgtcgcc cggctttcca
     3061 gaatctcatg cgcacgctgc gcatccttca gcggatattt ctgctgctcg gcgacatcga
     3121 ccttaatcac accgctggca atcaaagaga acagttcatt actggcctcg gttaattcct
     3181 cccgcgtggt gatatagcct tgcagggaag ggcgtgtcac atacaacgag cctttttgat
     3241 tgagaatgcc taagttcaca ccggtaaccg cacctgatga gttgccaaaa ctgaccatta
     3301 agccgcggcg ttgcaggcaa tccagcgacc gttcccaggt gtctctgccc acggaatcgt
     3361 acactacgcg cactttctta ccgccggtga tctctttcaa tcgctcgacc agattctctt
     3421 cacgatagtt aataacctgc cacgcgccgg cttttagcgc gctctgcgct ttttgcgcgg
     3481 ttcctacggt gccgatgagt ttcgcgccca gggcttttgc ccactggcag gcaattaagc
     3541 caatgccgcc agccgctgcg tggaacagga actgttcatc gggtttaatt tcataggttt
     3601 tgcgcagcag ataataaacc gttaagcctt tcaggaagga tgccgcagct tgctcaaaag
     3661 aaattgccgc aggcagaatc gccgctttat ccgcattaat gttatgcaca gagctgtaag
     3721 cgcctaacgc cgactgcgca tagactacat gatcgccagc cttaatatgc tttacaccac
     3781 tgccgacttt actcacgatg cctgccgctt cggtacctaa tccgctgggt agcgatggcg
     3841 gcgggtaaag gccgctgcgg atatacgtgt cgataaaatt gatgccgatg gctttatttt
     3901 cgacctggat ttcattctcc gccggatcgg caggagtgaa ctctacggct tgaagtactt
     3961 ccgggccacc gtgcttgtga aattcaattc gtgttgccat gtgtcctcca gaacgtatcg
     4021 tcagggtctg cttcatatgg taaagtttcg acccattctt tatttcggta actccattca
     4081 ctatggcagg aaataaaccc ttcaacaaac agcaggctga accccgcgaa cgcgatccac
     4141 aagttgccgg gctgaaagtg cctccgcact cgatcgaagc ggagcagtcg gtgttgggcg
     4201 gtttaatgct ggataacgaa cgctgggatg atgtagccga gcgtgtggta gcagacgatt
     4261 tttacacccg cccacaccgt catatcttta ctgaaatggc gcgtttgcag gaaagcggta
     4321 gtcctatcga tctgattacc cttgcggaat cgttggaacg ccaggggcaa ctcgatagcg
     4381 tcggtggttt tgcttatctg gcagagctgt caaaaaatac gccaagtgcg gcgaacatca
     4441 gtgcttatgc ggacatcgtg cgtgaacgtg ccgttgtccg tgagatgatc tcggttgcga
     4501 atgagattgc tgaagccggt tttgatccgc aggggcgtac cagcgaagat ctgctggacc
     4561 ttgctgaatc ccgcgtcttt aaaattgccg aaagtcgtgc aaacaaagac gaagggccga
     4621 agaacatcgc cgatgtgctc gacgcaaccg tggcgcgtat tgagcagttg tttcagcagc
     4681 cacacgatgg cgttaccggg gtaaacaccg gttatgacga tctcaacaaa aaaaccgctg
     4741 gcttgcagcc gtcggatttg atcatcgtcg ccgcgcgtcc gtcgatgggt aaaacaacat
     4801 ttgcgatgaa cctcgtcgaa aacgcggcaa tgttgcagga taaaccagta cttatcttct
     4861 cgctggagat gccatcagaa cagatcatga tgcgttctct ggcgtcgctg tcgcgcgttg
     4921 accagactaa aatccgtacc gggcagctcg atgatgaaga ctgggcgcgc atttccggca
     4981 ccatgggtat tttgctcgaa aaacgcaata tctatatcga tgactcttcc ggcctgacgc
     5041 caacggaagt gcgttcccgc gcacgccgta ttgcccgtga acacggcggc atcgggctta
     5101 tcatgatcga ctacctgcaa ctgatgcgcg taccggcgct ttccgataac cgtacgctgg
     5161 aaattgcaga aatctcccgc tcgctgaaag cactggcgaa agaactgaac gtgccggtgg
     5221 tggcgctgtc ccagttgaac cgttctctgg aacaacgtgc cgacaaacgt ccggtcaact
     5281 ccgacctgcg tgaatctggc tctatcgagc aggatgcgga cttgatcatg tttatctatc
     5341 gtgatgaggt gtatcacgaa aacagtgatt taaaaggcat cgcggaaatt attatcggta
     5401 aacaacgtaa cggcccaatc gggacggtac gcctgacctt taacggtcaa tggtcgcgct
     5461 tcgacaacta tgcggggccg cagtacgacg acgaataata attattttat gaattaggta
     5521 attaaagcaa acaattatca aggaacacaa atgcaagcgg caactgttgt gattaaccgc
     5581 cgcgctctgc gacacaacct gcaacgtctt cgtgaactgg cacctgccag taaaatggtt
     5641 gcggtggtga aagcgaacgc ttatggtcac ggtcttcttg agaccgcgcg aacgctcccc
     5701 gatgctgacg cctttggcgt agcccgtctc gaagaagctc tgcgactgcg tgcgggggga
     5761 atcaccaaac ctgtactgtt actcgaaggc ttttttgatg ccagagatct gccgacgatt
     5821 tctgcgcaac attttcatac cgccgtgcat aacgaagaac agctggctgc gctggaagag
     5881 gctagcttgg acgagccggt taccgtctgg atgaaactcg ataccggtat gcaccgtctg
     5941 ggcgtaaggc cggaacaggc tgaggcgttt tatcatcgcc tgacccagtg caaaaacgtt
     6001 cgtcagccgg tgaatatcgt cagccatttt gcgcgcgcgg atgaaccaaa atgtggcgca
     6061 accgagaaac aactcgctat ctttaatacc ttttgcgaag gcaaacctgg tcaacgttcc
     6121 attgccgcgt cgggtggcat tctgctgtgg ccacagtcgc attttgactg ggtgcgcccg
     6181 ggcatcattc tttatggcgt ctcgccgctg gaagatcgct ccaccggtgc cgattttggc
     6241 tgtcagccag tgatgtcact aacctccagc ctgattgccg tgcgtgagca taaagccgga
     6301 gagcctgttg gttatggtgg aacctgggta agcgaacgtg atacccgtct tggcgtagtc
     6361 gcgatgggct atggcgatgg ttatccgcgc gccgcgccgt ccggtacgcc agtgctggtg
     6421 aacggtcgcg aagtaccgat tgtcgggcgc gtggcgatgg atatgatctg cgtagactta
     6481 ggtccacagg cgcaggacaa agccggggat ccggtcattt tatggggcga aggtttgccc
     6541 gtagaacgta tcgctgaaat gacgaaagta agcgcttacg aacttattac gcgcctgact
     6601 tcaagggtcg cgatgaaata cgtggattaa tcgttctgta atatttgatt gtctgtgccg
     6661 gatgcggcgt gaatgcctta tccggccaat aaaatcctaa aaattcaata agttgatgtt
     6721 ctttcatgct cttataaagg tcgtgcctct ggcggatgta cgtttatcat tagtctcact
     6781 ctgttgctaa ttgccgttcg ctcctgaaca tccactcgat cttcgccttc ttccggttta
     6841 ttgtgtttta accacctgcc cgtaaacctg gagaaccatc gcgtgtttca aaaagttgac
     6901 gcctacgctg gcgacccgat tcttacgctt atggagcgtt ttaaagaaga ccctcgcagc
     6961 gacaaagtga atttaagtat cggtctgtac tacaacgaag acggaattat tccacaattg
     7021 aaagccgtgg cggaggcgga agcgcgcctg aatgcgcagc cccatggcgc ttcgctttat
     7081 ttaccgatgg aagggcttaa cagctatcgc catgccattg cgccgctgct gtttggtgcg
     7141 gaccatccgg tactgcaaca acagcgcgta gcaaccattc aaacccttgg cggctcaggg
     7201 gcattgaaag tgggcgcgga tttcctgaaa cgctacttcc cggaatcagg cgtctgggtc
     7261 agcgatccta cttgggaaaa ccacgtagca atattcgccg gggctggatt cgaagtgagt
     7321 acttacccct ggtatgacga agtgactaac agcgtgcgct ttaatgacct gttggcgacg
     7381 ctgaaaacat tacctgcccg cagtattgtg ttgctgcatc catgttgcca caacccaacg
     7441 ggtgccgatc tcactaatga tcagtgggat gcggtgattg aaattctcaa agcccgcgag
     7501 cttatcccat tcctcgatat tgcctatcaa ggatttagtg ccggtatgga agaggatgcc
     7561 tacgctattc gcgccattgc cagtgctgga ttacccgctc tggtgagcaa ttcgttctcg
     7621 aaaattttct ccctttacgg cgagcgcgtc ggcggacttt ctgttctgtg tgaagatgcc
     7681 gaagccgctg gccgcgtact ggggcaattg aaagcaacag ttcgccgcaa ctactccagc
     7741 ccgccgaatt ttggtgcgca ggtggtggct gcagtgctga atgacgaggc attgaaagcc
     7801 agctggctgg cggaagtaga agagatgcgt actcgcattc tggcaatgcg tcaggaattg
     7861 gtgaaggtat taagcacaga gatgccagaa cgcaatttcg attatctgct taatcagcgc
     7921 ggcatgttca gttataccgg tttaagtgcc gctcaggttg accgactacg tgaagaattt
     7981 ggtgtctatc tcatcgccag cggtcgcatg tgtgtcgccg ggttaaatac ggcaaatgta
     8041 caacgtgtgg caaaggcgtt tgctgcggtg atgtaatgca ggaaagcagg ctggagttac
     8101 ccagcctgca gtgaaattaa actgtcgtcg ctttcactat ttctttgtag atgatttttt
     8161 tgatgccgtt gttctacgtg agagataata aaacgttgtc agttctttta ttgttaagtt
     8221 tatcccaatt atctggaatt cctttcccta ttttttc
//
LOCUS       DAZXUK010000178         8236 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZXUK010000178 DAZXUK010000000
VERSION     DAZXUK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8236
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.178"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            290..1222
                     /gene="glaH"
                     /locus_tag="ACH3L8_003038"
     CDS             290..1222
                     /gene="glaH"
                     /locus_tag="ACH3L8_003038"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFT4086056.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1242..2510
                     /gene="lhgO"
                     /locus_tag="ACH3L8_003039"
     CDS             1242..2510
                     /gene="lhgO"
                     /locus_tag="ACH3L8_003039"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFT4086057.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2533..3981
                     /gene="gabD"
                     /locus_tag="ACH3L8_003040"
                     /pseudo
     CDS             2533..3981
                     /gene="gabD"
                     /locus_tag="ACH3L8_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            3989..5275
                     /gene="gabT"
                     /locus_tag="ACH3L8_003041"
                     /pseudo
     CDS             3989..5275
                     /gene="gabT"
                     /locus_tag="ACH3L8_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            5513..6913
                     /gene="gabP"
                     /locus_tag="ACH3L8_003042"
     CDS             5513..6913
                     /gene="gabP"
                     /locus_tag="ACH3L8_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFT4086058.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6934..7596
                     /gene="csiR"
                     /locus_tag="ACH3L8_003043"
     CDS             6934..7596
                     /gene="csiR"
                     /locus_tag="ACH3L8_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFT4086059.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7597..8046)
                     /gene="kbp"
                     /locus_tag="ACH3L8_003044"
     CDS             complement(7597..8046)
                     /gene="kbp"
                     /locus_tag="ACH3L8_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFT4086060.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8130..>8236)
                     /locus_tag="ACH3L8_003045"
     CDS             complement(8130..>8236)
                     /locus_tag="ACH3L8_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFT4086061.1"
                     /translation="LGKGFGWAFIINILLTLLGYIPGLIHAFWVQTRD"
BASE COUNT         1852 a         2171 c         2296 g         1917 t
ORIGIN      
        1 agtttactaa cggtcatttt gtagtgaagc catttactgt tttttatcga ccagataatc
       61 tgttctctaa tgttaactcc ccctaacctg ttgctttagt tattcatttc ctgtctcact
      121 ttgccttaat accctacgtt aaatgttact aatttgttgc ttttgatcac aataagaaag
      181 caatatgtcg cttttgtgcg catttttcag aaatgtagat atttttagat tatggctacg
      241 aaatgagcat cgccatgtca ccctacatct cataagagga tcgcttctga tgaatgcact
      301 gaccgccgta caaaataacg ctgtcgattc aggccaggac tatagcggat tcactctcat
      361 cccgtcggcg caatccccgc gtctgctgga actcaccttc accgaacaga cgaccaaaca
      421 gtttctcgaa caggttgccg aatggcccgt gcaggcgctg gagtacaaat cgtttctgcg
      481 ttttcgggta ggcaaaattc ttgacgatct gtgtgcgaat cagctgcaac cattgctgtt
      541 gaagaccctg ctaaaccgtg ctgaaggtgc gctgttgatc aatgcggtag gtatcgatga
      601 tgtggcgcag gcagatgaga tggtgaagct ggcaacggcg gtagcgcatc tgattggtcg
      661 ctcaaatttc gatgcgatga gcggtcagta ttacgcgcgt ttcgtggtga aaaatgtcga
      721 taactcagac agctatctgc gtcagccgca ccgcgtgatg gaactgcaca acgacggcac
      781 gtatgtcgaa gagatcaccg attacgtgct gatgatgaaa atcgatgact gggaacatct
      841 ggaccactat ttccgccatc cactggcgcg tcgcccgatg cgctttgccg cgccgccgag
      901 caaaaacgtc agcaaagatg ttttccatcc agtgttcgac gttgatcaac agggtcgccc
      961 ggtgatgcgc tatatcgacc agttcgtcca gccaaaagat tttgaagaag gcgtgtggtt
     1021 gagcgagctt tccgatgcca ttgaaaccag caaaggcatt ctttctgtac ccgttcccgt
     1081 tggcaaattc ctgttgataa acaacctgtt ctggctgcac ggtcgcgacc gctttactcc
     1141 gcacccggat ctgcgccgtg aactgatgcg tcagcgtggc tatttcgctt acgccactca
     1201 ccactaccag acgcatcagt aagcgcaaag gaattgagcg gatgtatgat tttgtgatta
     1261 ttggcggcgg catcatcggc atgtcgaccg ccatgcaact gattgatgtt tatccggatg
     1321 cccgcattgc gttgctggaa aaagagtccg gcccggcctg tcaccagacg ggccacaaca
     1381 gcggcgtgat ccatgccggg gtctattaca cgcccggcag cctgaaggcg cagttttgcc
     1441 tggcggggaa ccgtgccacc aaagcctttt gcgatcaaaa cggcattcgc tatgacaact
     1501 gcggcaagat gctggtcgcc acgtctcagc tcgaaatgga acggatgcgc gccttatggg
     1561 aacgcacagc ggcgaacggt atcgagcgcg agtggataaa cgccgtggaa ctgcgcgagc
     1621 gcgaaccgaa tatcaccggg cttggtggca tttttgtgcc gtccagcggc attgtcagct
     1681 accgcgaagt gacggcggca atggcaaaaa tcttccaggc cagaggcggc gagattattt
     1741 ataacgccga agtcagcgcc ctcagtgagc ataaaaacgg cgtggtgata cgcacccgtc
     1801 agggcggtga atatgaagca tcgacgctga ttagctgttc cgggctgatg gctgaccggc
     1861 tggtgaaaat gctcggcctc gaaccgggct ttattatctg cccgttccgt ggcgagtatt
     1921 tccgccttgc gccggagcat aaccagattg ttaaccacct gatttacccc attcccgacc
     1981 ccgcgatgcc atttttgggc gtgcatctca cccgcatgat cgacggcagc gtgaccgtcg
     2041 ggccaaacgc ggtgctggct ttcaaacgcg aaggctatcg caagcgcgac ttctcattta
     2101 gtgacacgct ggagattttg agctcgtcgg ggattcgccg ggtgctgcaa aaccatctac
     2161 gctcaggact gggcgagatg aaaaactcgc tgtgcaaaag cggctatctg cggctggtgc
     2221 aaaagtattg tccccggctt tcgttaagcg atctccagcc ctggcccgcc ggtgtgcggg
     2281 cgcaggcagt atcgccggac ggcaagctga ttgacgattt tctgtttgtc accaccccac
     2341 gcacgatcca cacctgcaat gcgccctccc cggcagcgac atcagcaatt cctattggtg
     2401 cgcatattgt cagcaaggta caaacgctgt tggcaagcca gagtaacccc ggacgcacgc
     2461 tgcgagcggc acgtagtgtg gatgccttac acgccgcatt taatcaataa cctttgaaaa
     2521 caggacgtaa caatgaaact taacgacagt aacttattcc gccagcaggc gttgattaac
     2581 ggggaatggc tggacgccaa caatggtgaa gtcatcgacg tcaccaatcc ggcgaacggc
     2641 gacaagctgg gtagcgtgcc gaaaatgggc gcagatgaaa cccgcgccgc tatcgacgcc
     2701 gccaaccgcg ccctgcccgc ctggcgtgcg ctcaccgcca aagaacgcgc caccattctg
     2761 cgcaactggt tcaatttgat gatgcagcat caggacgatt tagcgcgcct gatgaccctc
     2821 gaacagggta aaccgctggc tgaagcgaaa ggcgaaatca gctacgccgc ctccttcatt
     2881 gagtggtttg ctgaagaagg caaacgtatt tatggcgaca ccattcctgg tcatcaggcc
     2941 gataagcgcc tgattgttat caagcagccg attggcgtca ccgccgccat cacgccgtgg
     3001 aactttccgg cggcgatgat tacccgtaaa gccggtccgg cgctggcggc aggctgcacc
     3061 atggtgctga agcccgccag tcagacgccg ttctctgcgc tggcgctggc ggaactggcg
     3121 attcgcgcgg gcattccggc tggcgtgttt aacgtggtca ccggttcggc aggcgcggtc
     3181 ggtaatgaac tgaccagcaa cccgctggta cgcaaactgt cgtttaccgg ttagaccgag
     3241 attggccgcc agttaatgga acagtgcgcg aaagacatca aaaaagtgtc gctggagctg
     3301 ggcggcaacg cgccgtttat cgtctttgac gatgccgatc tcgataaagc cgtggaaggc
     3361 gcgctggcct cgaaattccg caacgccggg caaacctgcg tctgcgccaa ccgtttatac
     3421 gtgcaggacg gcgtgtatga ccgctttgcc gaaaaattac agcaggcggg gagcaaactg
     3481 cacatcggcg acgggctgga gaaaggcgtc accatcgggc cgctgatcga tgaaaaagcg
     3541 gtagcaaaag tggaagagca tattgccgat gcgctggaga aaggtgcgcg tgtggtttgc
     3601 ggcggtaaag cgcacgaacg cggcggcaac ttcttccagc caaccattct ggtggacgtt
     3661 ccggccaacg ccaaagtgtc gaaagaagag acgttcggcc ccctcgcccc actgttccgt
     3721 tttaaagatg aagccgatgt gattgcacaa gccaatgaca ccgagtttgg tcttgccgcc
     3781 tatttctacg cccgtgattt aagccgcgtc ttccgcgtcg gcgaggcgct ggagtacggc
     3841 atcgtcggca tcaataccgg cattatttcc aatgaagtgg ccccattcgg cggcatcaaa
     3901 gcctcgggtc tgggtcgtga aggttcgaag tatggcatcg aagattactt agaaatcaaa
     3961 tatatgtgca tcggtcttta actggagaat gcgaaggaac agcaataaag agttaatgca
     4021 gcgccgcagt caggcgattc cccgtggcgt tgggcaaatt cacccgattt tcgctgaccg
     4081 cgcggaaaac tgccgggtgt gggacgttta aggccgtgag tatcttgatt tcgcgggcgg
     4141 gattgcggtg ctcaataccg ggcacctgca tccgaaggtg gtggccgcgg tggaagcgca
     4201 gttgaaaaaa ctgtcgcaca cctgcttcca ggtgctggct tacgagccgt atctggagct
     4261 gtgcgagatt atgaatcaga aggtgccggg cgatttcgcc aagaaaacgc tgctggttac
     4321 gaccggttcc gaagcggtgg aaaacgcggt aaaaatcgcc cgcgccgcca ccaaacgtag
     4381 cggcaccatc gcttttagcg gcgagtatca cgggcgcacg cattacacgc tggcgctgac
     4441 cggcaaggtg aatccgtact ctgcgggcat ggggctgatg ccgggtcatg tttatcgcgc
     4501 gctttatcct tgcccgctgc acggcataag cgaggatgac gctatcgcca gcatccaccg
     4561 gatcttcaaa aatgatgccg cgccggaaga tatcgccgcc atcatgattg agccggttca
     4621 gggcgaaggc ggtttctacg ccgcgtcgcc agcctttatg cagcgtttac gcgctctgtg
     4681 tgacgagcac gggatcatgc tgattgccga tgaagtgcag agcggcgcgg ggcgtaccgg
     4741 cacgctgttt gcgatggagc agatgggcgt ggcgccggat cttaccacct ttgcgaaatc
     4801 gatcgcaggc ggtttcccgc tggcgggcgt caccgggcgc gcggaagtga tggacgctgt
     4861 tgcaccgggc ggtctgggcg gcacctatgc cggtaacccg attgcctgcg tggctgcgct
     4921 ggaagtgttg aaggtgttcg agcaggaaaa tctgctgcaa aaagccaacg atctggggca
     4981 gaagctaaaa gacggattgt tggcgatagc cgaaaaacac ccggagatcg gcgacgtacg
     5041 cgggctggga tcgatgattg ccatcgagct gtttgaagat ggcgatcaca gcaaaccgga
     5101 cgcaaaactc accgccgaga tcgtggcacg cgcccgcgat aagggcctga ttcttctctc
     5161 ctgcggcccg tattacaacg tgctgcgcat ccttgtacca ctcaccattg aagacgctca
     5221 gatccgtcag ggtctggaga ttatcggtca gtgttttgat gaggcgaagc agtagcaccg
     5281 tccccatgca ggaggcgacg ctgcgcgtct tgtccggcct acgagaatcc aagtcggata
     5341 aggcgtttac gccgcatccg acaatctgta cgtgaacagg aataaatcta tgtcggccgg
     5401 ataaggcgga gccgctctcc ggcaaaaaga atcaataaca attaaacgcg tgacccgacg
     5461 cgggaaatgt cggggcgctc tcccaagtga cacactttcg agaggattca ggatggggca
     5521 atcatcgcaa ccacacgagt taggcggcgg gctgaaatca cgccacgtca ccatgttgtc
     5581 tattgccggt gttatcggcg caagtctgtt tgtcggttcc agcgtcgcca tcgccgaagc
     5641 ggacccggcg gtattactgg cctatctgtt cgccggatta ctggtggtta tgattatgcg
     5701 gatgctggcg gaaatggcag ttgccacgcc cgataccggt tcgttttcca cctatgccga
     5761 taaagccatt ggccgctggg cgggctatac catcggctgg ttgtactggt ggttttgggt
     5821 actggttatc ccactggaag ccaacatcgc cgccatgatc ctgcactcgt gggttccagg
     5881 cattcccatc tggttatttt ccctcgtcat taccctcgcc ttaactggca gtaatttatt
     5941 aagcgttaaa aactacggcg aatttgagtt ctggctggcg ctgtgcaaag tcatcgctat
     6001 cctggccttt attttccttg gtgcagtcgc aattagcggt ttttaccctt atgccgaagt
     6061 gagcgggatc tcaagattgt gggatagcgg cggctttatg cccaacggtt tcggtgcggt
     6121 attaagcgcg atgttgatca ccatgttctc gtttatgggc gcagaaattg tcaccattgc
     6181 cgccgcggaa tccgacatgc cggaaaaaca tattgtccgc gccaccaact cggttatctg
     6241 gcgtatttct atcttctatt tgtgctctat ttttgtcgtc gtggcattaa taccgtggaa
     6301 tatgccgggg ctgaaagccg ttggatctta tcgctcggtt ctggaattgc tcaatattcc
     6361 ccatgcgaaa ttaatcatgg actgtgtgat attactttcc gtaaccagtt gcctgaactc
     6421 ggccctgtat accgcctcaa ggatgctcta ctccttaagc cgtcgcggtg atgctcccgc
     6481 ggtaatgggt aaaatcaacc gcagtaaaac cccgtacgtg gcggtgttac tctccaccgg
     6541 cgcagcattc ttaacggtgg tggtgaacta ttacgcgcct gcgaaggtat ttaaatttct
     6601 gatcgacagc tccggcgcta tcgccctgct ggtttattta gtcatcgccg tttcacagtt
     6661 gcggatgcgc aaaattctgc gagcagaagg aagcgaaatt cgcttgcgaa tgtggcttga
     6721 tccctggctc acctggctgg tcatcggctt tattaccttt gtgttggtag tgatgctatt
     6781 ccgtccggcg caacagttag aagtgatctc caccggctta ttagcgatag ggattatctg
     6841 taccgtgcca attatggctc gctggaaaaa gctggtattg tggcaaaaaa cacccgttca
     6901 taatacgcgc tgataatgat caggagtcac accatgacca ttacgtctct ggatggctat
     6961 cgctggctga agaacgatat tattcgcggt aattttcaac cggatgaaaa attacggatg
     7021 agtttactga catcgcgtta tgcacttggc gttgggccgt tgcaggaagc actttctcaa
     7081 ctggtggcgg aacggctggt cacggtggtg aatcaaaaag ggtatcgggt ggcgtctatg
     7141 tcagagcagg agctgctcga tattttcgac gcccgcgcca atatggaagc gatgctggtc
     7201 agtctggcga ttgcccgcgg tggcgatgaa tgggaggcag acgttctcgc aaaagcgcat
     7261 ctgctcagta agctggaagc ctgtgacgcc agcgagaaaa tgcttgatga gtgggatctg
     7321 cgtcatcagg cgtttcatac agcaattgtg gcgggctgtg gttctcacta tttgctgcaa
     7381 atgcgtgaac ggttgtttga tctggcggcg cgttatcgat ttatctggct gcggcgaacg
     7441 gtgctttcgg tggaaatgct ggaggataaa cacgatcagc accagaccct gactgcggcg
     7501 gtactgccgc gagatacagc gcgcgccagt gagttaatgc gccagcattt actgacgcca
     7561 attcccatta tccagcaggc gatggctggc aattaactac tcttccggaa tacgcaacac
     7621 ttgccccgga taaattttat ccgggctttt tagcatcggt ttattcgctt cgaagatttt
     7681 attgtacaga ttagcgttac cgtagacctg tttggaaatg gcactcagag tgtcgccaga
     7741 cttaacggta taaaactggc tggcagtggc tggtgtcgcc gttttcacct gatcatcaac
     7801 actggcaata ccggaaatat tccccaccgc aacaaggatt ttctccttcg cctcctgact
     7861 caggccgtca ccagtgaccg tcgctttgcc gtcggcaatt tgaatgttca ctttatcggc
     7921 atcaggtata ccggttttgc tcagatgctc ctgcaccttc ttcgcctgat cgtctttatc
     7981 gtgctgacct gtaaccgcat cccagagttt ttctccggca tctttaacaa aattgaacag
     8041 acccatagct acctcattcg ttaacggaaa cactcagaaa gtgtagcaga ggaacgcggc
     8101 gcgttaacgc cgcgggggtg agcacaggtt taatcgcggg tttgcaccca gaacgcgtga
     8161 atcaaaccgg ggatataccc aagcagtgtc agcagaatat taataatgaa cgcccaaccg
     8221 aaccctttac cgagca
//
LOCUS       DAZXUK010000179         8224 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZXUK010000179 DAZXUK010000000
VERSION     DAZXUK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8224
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.179"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..305
                     /locus_tag="ACH3L8_003046"
     CDS             <1..305
                     /locus_tag="ACH3L8_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFT4086062.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            277..2204
                     /locus_tag="ACH3L8_003047"
                     /pseudo
     CDS             277..2204
                     /locus_tag="ACH3L8_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2188..2391
                     /locus_tag="ACH3L8_003048"
     CDS             2188..2391
                     /locus_tag="ACH3L8_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFT4086063.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2388..3981
                     /locus_tag="ACH3L8_003049"
                     /pseudo
     CDS             2388..3981
                     /locus_tag="ACH3L8_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3971..5476
                     /locus_tag="ACH3L8_003050"
     CDS             3971..5476
                     /locus_tag="ACH3L8_003050"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFT4086064.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5513..5860
                     /locus_tag="ACH3L8_003051"
     CDS             5513..5860
                     /locus_tag="ACH3L8_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFT4086065.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5918..6946
                     /locus_tag="ACH3L8_003052"
     CDS             5918..6946
                     /locus_tag="ACH3L8_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFT4086066.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6998..7384
                     /gene="gpFI"
                     /locus_tag="ACH3L8_003053"
     CDS             6998..7384
                     /gene="gpFI"
                     /locus_tag="ACH3L8_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFT4086067.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7396..7773
                     /locus_tag="ACH3L8_003054"
     CDS             7396..7773
                     /locus_tag="ACH3L8_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFT4086068.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7760..>8224
                     /locus_tag="ACH3L8_003055"
     CDS             7760..>8224
                     /locus_tag="ACH3L8_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFT4086069.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1905 a         1932 c         2541 g         1846 t
ORIGIN      
        1 tactcattgc cagatgggaa ctgaccgcag aacaggctgt cgcacagcag ttaaaaaatc
       61 aggtgtcaaa aggaaatctt atcgataccg gattttgtat ttttgccctt agtaagctgg
      121 cgatggcttt atccagtacg cttgactcca ttcctttgtc catgcaacga cagttcccgg
      181 atttaacgcc gcgtcatatt gatcatctga aaatccttat tgctaaggga gcgaatcagt
      241 gcgcgcgtgc gggggataaa ttaccggatt tactcgatga atatatcaga acaacaactg
      301 agtaatatga tggtggcagt cactatcgca ctgcagccac tggtcagggt attgccggtg
      361 acggctgtag aatgggccga tcaaaattat tacctgccta aagaatcgtc atacggtgag
      421 ggggaatgga aaacgctgca gtttcaggtc gccatcatga actgcatggg caatgacgag
      481 attcgtacag ttaacctgat taaatctgcc cgtgttggtt atacaaaaat ggtgcttggt
      541 gtgatcgggt attttattga gcacaaatcc cgcaacactc ttctttttca gccgacggac
      601 tcagctgctg aagattttat gaagtctcac gtggaagcaa caattcgtga tgttccttgc
      661 ctgaaaaaac tttctccctg gctgggccgg aaacaccggg ataatacgct cacactgaag
      721 cgtttttcct ctggtgtggg gttctggtgt ttgggtggtg ctgctgccaa aaactaccgt
      781 gaaaaatctg tcgatgtggt ctgctatgac gaactctcct cgtttgaacc ggatgtggaa
      841 aaagaaggtt cgccaacact gcttggcgat aaacgtattg aaggttcggt atggccaaaa
      901 tccatacgcg gttcaacgcc aaaaattaaa ggctcctgtc agattgaaaa agctgcgaat
      961 gaatctgcgc atttcatgcg gttttatgtc ccttgccctc attgcgggga ggagcagtat
     1021 ctgaagtttg gcgatgatgc gacgtcgttt ggcctgaaat gggagaaggg taaaccggaa
     1081 acggtgtatt acctgtgtga acataatggc tgcgtgatcc gtcagtctga acttgatcag
     1141 accggggggc gctggatttg tgacaatacc gggatgtgga cgtgtgacgg tctgacgttt
     1201 ttcagtgctt cgggtaatga aattccgccg ccgcgctcca tcactttcca tatatggacg
     1261 gcgtacagtc cgttcaccac cagggtacag atagtctgtg actggctgga tgcactgaaa
     1321 gatcccaacg gcgtgaaaac ttttgtgaac accacgctgg gcgagacctg ggaagaggcc
     1381 gtgggcgaaa aactcgatca ccaggtactg atggataagg ttgtgcctta cacggcaacg
     1441 gtgcctgtcc gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag
     1501 atgtatgtct gggggtgggc tccgggagag gaagcctttc tggtggataa aatcatcatc
     1561 atgggacgtc ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caataaaaaa
     1621 taccgccatg cagacggtac cgaaatgacc atttcccgtg tctgctggga tatcgggggg
     1681 atcgatggcg aaatcgtgta tcagaggtca aaaaaacacg gtattttccg ggtactgccg
     1741 gtaaaaggtg cctctgtcta tggcaagccg gtgatcacca tgccgaaaac ccgcaatcag
     1801 cggggtgtgt atctgtgtga ggtggggacg gataccgcaa aagaaattct ctatgcccga
     1861 atgaaggccg atcccacccc tgtgaatgag gccacatcgt atgccatccg ctttcctgat
     1921 aatccggaga ttttttcgca gacagaggca caacaactgg tggcggagga actggtggaa
     1981 aagtgggaaa aaggaaagat gcgtctgttg tgggataaca aaaagcggcg taacgaagcg
     2041 ctggactgcc tggtgtatgc ctatgcggca ttacgtgtgt ccgtgcaacg ctggcaactt
     2101 gatctggctg tgcagttgca tcccgcaagg atgcaactgc acaaccgacc cttgaagaac
     2161 tggcagcgaa gctgtccgga ggagtgaatg gttacagtcg ctgaactgca ggcgctgcgt
     2221 caggcgcgcc ttgatttatt aaccggtaaa cgggttgtgt cggtacagaa agacggtcgc
     2281 aggatcgaat atacggcagc gtccctgaat gagcttaacc gtgcgatcaa cgatgcggag
     2341 tcggttctgg gtacgacccg acgccgccga ccgctgggag tgaggttatg aaacgaacgc
     2401 ctgtcctgat tgatgtgaac ggcgttccgc ttcgtgagag tctcagctac aacggaggcg
     2461 gcgcaggatt tggcgggcaa atggcagagt ggttaccacc ggcgcagagt gccgatgcgg
     2521 ccctgttgcc tgcattgcgt ctggggaatg cccgggcaga tgatctggtg cgcaataacg
     2581 gtatcgcggc taatgcggtg gccctgcata aggatcatat cgtcgggcac atgtttctta
     2641 tcagctaccg tctgaactgg cgttggctgg ggatgcggga gaccacggca aaaagttttg
     2701 tcggggaggt ggaagcagcc tggtcggaat acgcagaagg aatgtttggc gagatcgacg
     2761 tggaagggaa acgcacgttt acggaattta tccgtgaagg ggtgggcgtg catgccttta
     2821 acggcgaaat ctttgtgcag ccggtctggg atacggaaac cacgcagtta ttccgtacgc
     2881 gttttaaagc cgtgagtccg aaacgggtgg acacgccagg acacggtatg gggaaccact
     2941 ttctgcgggc cggggttgag gtcgatcggt atgggcgggc gattgcgtac catatctgtg
     3001 aggatgattt tccgttctct ggtgccggac gctgggagcg gatcccgcgt gagcttccca
     3061 ccgggcgtcc ggccatgctg catatttttg agccggtgga ggacgggcag acccgtgggg
     3121 ctaatcagtt ttacagcgta atggagcgga tgaagatgct ggactccctg caggccacgc
     3181 agcttcagtc ggccattgtg aaggcgatgt atgcagccac aatcgaaagt gaacttgata
     3241 ccgaaaaggc atttgaatat atcgcggggg caccgcaagg tcagcaggat aatccgctta
     3301 ttaatattct ggagaagttc acccgctggt atgacacgaa taacgtgacg ctgggcggtg
     3361 ttaaaattcc gcaccttttt tcctggcgat gcgctgaacc tgcagacagc gcaggattca
     3421 gacaatggat tttcggcgct tgaacaggcg ctgctgcggt atattgcagc cggtcttggc
     3481 gtttcctacg aacagttgtc ccgtgattac tcgaaggtca gttactcaag tgcccgcgcc
     3541 tccgccaatg agtcgtggcg ctattttatg gggcggcgaa aatttattgc ggcccggctg
     3601 gccacgcaga tgttttcctg ctggctggaa gaggcgcttc ttcgggggat tattcgtccg
     3661 ccacgggcgc gttttgattt ttaccaggcg cgttcagcct ggtcacgggc agagtggatt
     3721 agctccggaa gaatggccat tgacgggctc aaggaggtcc aggaatcggt gatgcgcatt
     3781 gaggccggac tgagcacgta tgagaaagaa ctggcactga tgggcgaaga ttatcaggac
     3841 attttccgcc agcaggtcag ggaatccgca gagcgcgaaa aagccggact ctcacgtccg
     3901 gtgtggatag cccaggcgta tcagcagcag atagcggaga gtcgcaggcc ggaagaggag
     3961 acaacatcac gtgagacgta atctttcaca tattatcgct gcggcattca atgagccgct
     4021 gcttctggag cccgcctatg cgcgggtttt cttttgcgcg ctcgggcgtg agatgggcgc
     4081 agcaagcctt tcggtaccgc aacagcaggt acagcttgac gctcccggga tgctggctga
     4141 aacggacgag tacatggccg gaggcaaacg acctgcccgt gtttaccggg tggtgaacgg
     4201 tattgctgta ctgccggtga ccggcacgct ggtacaccgg ctgggtggca tgcggccatt
     4261 ttccggaatg actggctatg acggcattgt cgcctgtctt cagcaggcaa tggcggacac
     4321 tcaggtacgg ggcgtactgc tggacattga cagttctggc gggcaggcca ccggcgcgtt
     4381 tgactgcgct gacatgattt accgtctccg gcagctgaag cctgtctggg cactgtgcaa
     4441 tgacacggcc tgttcagccg ccatgttact ggcatcggcc tgctcccgac ggctggttac
     4501 ccagacatcc cgtatcggct ccattggggt gatgatgggc catgtcagct atgccggtca
     4561 tctggcacag gccggtgtgg atatcacgct gatttacgcc ggatcgcaca aggtggatgg
     4621 caatcagttt gaatccctgc cagcaaaggt tcgccaggac atgcagcagc gcattgatgc
     4681 ggcgcgccgg atgtttgctg aaaaagtggc gatgtatacc ggtttatctg tggatgcggt
     4741 tatgggaaca gaggccgccg tttttgaagg tcagtccggt attgaggccg aactggcgga
     4801 tgaattaatc aatgcgtcgg acgccatcag cgtgatggct gcgacgctga acacacacga
     4861 tacaggaggc actatgccgc aattaactgc aacggaggct gccgcgcagg agaaccagcg
     4921 agtgatgggg atcctgacgt gtcaggaagc gaaaggacgt gaacaactgg ccacgatgct
     4981 ggcagggcaa cagggtatga gcgttgagca ggcccgggcg attctggccg tggcggcacc
     5041 gcaacaatct ggtgtatctg cgcagagtga agccgatcgc attatggcat gtgaagaggc
     5101 gaaaggacgt gaacaactgg cggcaacgct ggcggcgatg ccggagatga cggtggaaaa
     5161 agcccgtccg attctggctg cttcaccgca ggcgaatgcc ggaccctcac ttcgtgatca
     5221 gattatggcc ctggatgagg caaaaggggc agaggcgcag gctgaaaaac tggcggcctg
     5281 tccggggatg accgtggaga atgcccgggc tgtactggcg gcagcctcag gcagggcaga
     5341 accggtttct gcatccacaa ccgccatgtt tgaacatttc atggcgaacc attcaccggc
     5401 agcggtacag ggtggcgtgc cacagacgtc agcagacggt gatgcggacg tgaaaatgct
     5461 catggccatg ccatgaagtc agtgctgacc atcaatatga ggtttttaca atatggtaac
     5521 gaaaaccatc actgaacagc gtgcggaagt acgtattttt gccggtaatg atccggccca
     5581 taccgccaca ggctgcagcg ggatttcctc ggcaacaccg gctctgacgc ccctgatgct
     5641 ggatgaggat accgggaaac tggtggtctg ggacggacag aaggcaggcc atgtggttgg
     5701 catactggta ctgccgcttg aaggtacaga gacggtgctg acgtattaca agtcggggac
     5761 ctttgcgacg gaggccatcc actggcctga gagagtggat acacacaaaa aggcgaatgc
     5821 ctttgccggc agtgccctga gtcacgcagc gctgccgtaa cattttatca ggtcactgcg
     5881 gtagcctgac tgatttctga atgaaaggaa ctgatttatg ggattgttta cgacccgcca
     5941 gttactcggt tataccgaac aaaaagttaa atttcgtgcg ctgtttctgg agctgttttt
     6001 ccgccgtacg gtgaatttcc ataccgaaga ggtaatgctg gacaaaatta ccggaaaaac
     6061 gccggtggcg gcctatgtct ccccggttgt tgaaggaaag gtgttgcgtc accgtggcgg
     6121 tgaaactcgt gtactgcgcc cgggctacgt taagccgaaa cacgaattta attaccagca
     6181 ggcggtggag cgtcttcccg gtgaagatcc ggcccagctg aacggcccgg cttaccgtcg
     6241 cctgcgtatc atcaccgata acctcaaaca ggaagagcac gccattgtcc aggtggaaga
     6301 gatgcaggcg gtgaatgccg tgctgtatgg caaatacacc atggaaggag agcagtttga
     6361 aactgttgag gtcgattttg gccggtcggt gggaaataac attacgcagg caggagggac
     6421 ggagtggagc gctcaggacc gcgatacctt tgatccgacg catgatctgg atgcgtactg
     6481 tgattttgct tccggcacca tcaatattgc cattatggat ggtaaggtct ggcgtctgct
     6541 gaatggcttt aagctgttcc gcgaaaaact ggatacccgt cgtggctcaa attcacagct
     6601 ggaaacggca gtgaaagacc tgggggcggt ggtgtccttc aaagggtatt atggtgatct
     6661 ggcaatcgtg gtggcaaaaa cgtcctatgt ggcagaggac ggtaccgaaa aacgttatct
     6721 gccggacggc acactggtac tgggaaatac ggccgcagag ggtattcgtt gctacggtgc
     6781 cattcaggat gcgcaggcat tgtctgaagg cgtggtggcg tcttcccgtt atccgaaaca
     6841 ctggctgacc gtgggtgatc cggcgcgtga attcaccatg acgcagtccg cgccgctgat
     6901 ggtgctgccg gatccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcgggag
     6961 ctaaatgctc ccgtttttca ttcaatcagg agcgggtatg gctacgaaag aagaaaacct
     7021 gaaacgtctt tgtgagctgg ctgagaggct ggggcgtgaa ccggatgtct ccggtagtgc
     7081 ggcagatatt gcgcagcgag tggcggaact ggaggaagag cttggagatg cgggggaggt
     7141 cgcagagccg gatacgtact caccatcgaa agatgatccg accgcgcata aaggcgagtc
     7201 agttcctgaa cagccaggca ttgttaccgg agacgaaacg gggcaggtta cggtggtggc
     7261 actggcgaca ctgcatacaa aatgtaatgg ttcagtgata tttgtttcac cgggaacgtc
     7321 gtttcgtgtt tctgctgagg tggctgccag catggcggca ggtggtctgg caaaaaggca
     7381 gtaacaggag atgtcgtgga cggtttcgat aatctgtttg atgctgcgct tgccggtgtc
     7441 aatgaagtca ttctccgtga tatggggatc agcgccgtca ttacgtccgg agaacttgag
     7501 gggacacacc tgacaggggt ttttgatgat cccgaaagta tttcttttgt tgccgggggg
     7561 atccggcttg aggattcctc accgtgcctg tttgtgaaaa cggcagatat atcgcagtta
     7621 cgccgtcagg atacgttaac tatcggggat gattctttct ttgtggatcg tattactccg
     7681 gatgacgggg gatgctgtta tatccggtta aggcggggca gtcctgcccc acagaataat
     7741 gccagaatga ggcgatatga tgaagggact tgagaatgcc atccgcaatc tgaacagcct
     7801 cgatacccgc atggtgccac agaccagcgc atgggcggta aaccgtgtgg cggcaaagat
     7861 tgtttctgtc gccacacggc aggttgcgca gaataccgtt gccggggata accaggtgaa
     7921 aggtattccc ctgaaaacgg tcagggagcg tgtcaggctg cttaaagcca gcccctcagg
     7981 aaaaatgtat gcccgcatgc gtgttaaccg gggtaacctg cccgccatta agctgggtac
     8041 cgcacaggtc agactggccc gctcccggca tggcagcaac tcacgtcatc gtggcagcgt
     8101 actgaaagtg gggaaatacc ttttccggga tgcgtttatt cagcaactgg cgaatggtcg
     8161 ctggcatgtg atgcggcgta ttgacggcaa aaatcgttac cccattgatg tggtgaaaat
     8221 cccg
//
LOCUS       DAZXUK010000180         8099 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZXUK010000180 DAZXUK010000000
VERSION     DAZXUK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.180"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(22..594)
                     /locus_tag="ACH3L8_003056"
     CDS             complement(22..594)
                     /locus_tag="ACH3L8_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFT4086070.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="ACH3L8_003057"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="ACH3L8_003057"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFT4086071.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="ACH3L8_003058"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="ACH3L8_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFT4086072.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="ACH3L8_003059"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="ACH3L8_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFT4086073.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="ACH3L8_003060"
     CDS             complement(3556..5337)
                     /locus_tag="ACH3L8_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFT4086074.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="ACH3L8_003061"
     CDS             complement(5330..7102)
                     /locus_tag="ACH3L8_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFT4086075.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="ACH3L8_003062"
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="ACH3L8_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFT4086076.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..>8099)
                     /locus_tag="ACH3L8_003063"
     CDS             complement(7744..>8099)
                     /locus_tag="ACH3L8_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HFT4086077.1"
                     /translation="TRLQIQLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQH
                     LGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHY
                     LTHWLDYPHLPYSPE"
BASE COUNT         2006 a         2331 c         1995 g         1767 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacagtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catggcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaa
//
LOCUS       DAZXUK010000181         8049 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZXUK010000181 DAZXUK010000000
VERSION     DAZXUK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.181"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..499
                     /locus_tag="ACH3L8_003064"
     CDS             <1..499
                     /locus_tag="ACH3L8_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="metalloprotein; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFT4086078.1"
                     /translation="LHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAIVLDTAC
                     WAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQADMFSNGG
                     TDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLTRETVVQ
                     LTDFR"
     gene            524..3019
                     /gene="ptsP"
                     /locus_tag="ACH3L8_003065"
     CDS             524..3019
                     /gene="ptsP"
                     /locus_tag="ACH3L8_003065"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFT4086079.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(3022..3879)
                     /locus_tag="ACH3L8_003066"
                     /pseudo
     CDS             complement(3022..3879)
                     /locus_tag="ACH3L8_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3892..4626)
                     /gene="ypdB"
                     /locus_tag="ACH3L8_003067"
     CDS             complement(3892..4626)
                     /gene="ypdB"
                     /locus_tag="ACH3L8_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFT4086080.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(4641..6127)
                     /locus_tag="ACH3L8_003068"
                     /pseudo
     CDS             complement(4641..6127)
                     /locus_tag="ACH3L8_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
     gene            6504..7742
                     /gene="alaC"
                     /locus_tag="ACH3L8_003069"
     CDS             6504..7742
                     /gene="alaC"
                     /locus_tag="ACH3L8_003069"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFT4086081.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            complement(7807..7878)
                     /gene="ypdK"
                     /locus_tag="ACH3L8_003070"
     CDS             complement(7807..7878)
                     /gene="ypdK"
                     /locus_tag="ACH3L8_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFT4086082.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
BASE COUNT         1955 a         2037 c         2169 g         1888 t
ORIGIN      
        1 gctgcacgac gccgaactgc ctgcggaagt gtggctggtg gcaagttcca gcgaagaggt
       61 gggattacgc ggcgggcaaa ctgctacccg cgcggtgtcg ccggacgtcg ccattgtgct
      121 tgataccgcc tgctgggcga aaaactttga ttatggcgcg gctaaccatc gtcagattgg
      181 taacgggccg atgctggtgt taagcgataa atcgctgatt gcgccgccaa aacttaccgc
      241 gtggattgaa accgtggcgg cagaaattgg cgttccgttg caggcggata tgttcagtaa
      301 cggcggcacg gacggtggag cggtgcactt aaccggtact ggcgtaccca cattggtaat
      361 ggggcctgcc acccgccacg gacattgcgc ggcatcgatt gccgattgcc gcgacatttt
      421 gcagatggag caacttttat ctgcccttat tcaacgtctt acgcgtgaga cggttgttca
      481 actgacggat ttcagatgat ctcctgatta acccggagcg gttatgttaa cgattcaatt
      541 tctctgtcct ttgcctaacg gtctacatgc tcgtccggcg tgggaactta aagaacagtg
      601 cagccagtgg caaagcgaaa tcacctttat caaccatcgc cagaacgcaa aggctgatgc
      661 taaaagctcg ctggcgttga ttggaactgg caccttattt aatgacagtt gcagcctgaa
      721 cattagcggc agcgatgaag agcaggcgcg gcgcgtgctg gaagagtaca tcctggtgcg
      781 ctttatcgac agcgacagcg ttcagccgac gcaggcagaa ctgacggcgc atccgctgcc
      841 gcgttcatta agccgcctta acccggattt actgtacggc aatgtgctgg caagcggcgt
      901 cggcgtgggt acgctgaccc tgttacaaag cgacagcctc gacagttatc gggcaatccc
      961 cgccagtgcg caagattcca cccggctgga gcacagcctg gcaacgcttg ccgagcaact
     1021 gaatcagcaa ttgcgtgagc gtgacggcga aagcaaaact atcctcagcg ctcatttgtc
     1081 gctgattcag gatgatgaat ttgcaggcaa tatccgtcgc ctgatgacag aacagcatca
     1141 gggactgggg gcggcgatca tcagcaatat ggagcaggtt tgcgccaaac tatctgcttc
     1201 tgccagcgat tatctgcgcg agcgggtcag cgacattcgc gatatcagcg aacagttgct
     1261 gcatatcacc tggccggaac tgaagccgcg caacaatctg gtgctggaaa aaccgaccat
     1321 tctggtggct gaagatttaa ccccaagcca gtttttgagc cttgatttga aaaatcttgc
     1381 gggtatgatt ctggagaaaa ccgggcgcac ctcgcataca ctgattctgg cccgtgcctc
     1441 ggcgatcccg gtactgagtg gcttgccgct ggatgcgatt gcccgttatg ccgggcaacc
     1501 tgcagtgctt gacgcccagt gcggcgtgct ggcgattaac ccgaatgacg cggtgagcgg
     1561 ttattatcag gtcgcgcaga cgctggcgga taaacgccaa aaacaacagg cgcaggctgc
     1621 cgcgcagctg gcctattccc gtgataacaa gcgtattgat attgcggcga atatcggcac
     1681 cgctctggaa gcgccaggcg cgtttgccaa cggcgcggaa ggtgtcgggc tgttccgtac
     1741 cgaaatgctc tatatggatc gcgacagcgc gccggacgag caggagcaat ttgaagccta
     1801 ccagcaggtg ctactggcgg cgggcgacaa gccgattatc ttccgcacga tggacatcgg
     1861 cggcgataaa agcattcctt atctgaatat tccccaggaa gagaacccgt tcctcggcta
     1921 tcgcgcggta cgtatttacc cggaatttgc tggcctgttc cgcactcaac tgcgggccat
     1981 tttgcgcgcc gccagtttcg gcaacgccca gttgatgatc ccgatggttc acagcctcga
     2041 tcagatctta tgggtgaaag gcgagatcca aaaagcgatc gttgagctta agcgcgatgg
     2101 cctgcgtcat gcagagacga ttacgcttgg gatcatggtg gaagtgccgt cggtgtgcta
     2161 catcatcgac cacttctgcg atgaggtcga tttcttcagt atcggctcca acgatatgac
     2221 ccagtatctg tatgcggtcg atcgtaataa cccgcgcgta tcgccgctat ataacccgat
     2281 tacgccatcg ttcctgcgca tgttgcagca aatagttacc actgcgcatc agcggggcaa
     2341 atgggtaggc atttgcggtg aactgggcgg tgaaagccgt tatctgccgc tactgcttgg
     2401 gctgggcctg gatgagctga gtatgagtag cccgcgtatt ccggcggtga aaagccagct
     2461 tcgtcaactg gatagcgagg cgtgtcggga actggcgcgt caggcatgtg aatgccgcag
     2521 tgcgcaggaa attgaagcgt tactcaccgc ctttacgccg gaagaagacg ttcgcccact
     2581 gctggcgctg gagaatatct ttgttgatca ggattttagc aataaagagc aggcgatcca
     2641 gttcctgtgc ggcaacctcg gcgttaacgg gcgcactgaa catccgtttg agctggaaga
     2701 agatgtctgg cagcgggaag agattgttac caccggcgtt ggttttggcg tagcgatccc
     2761 gcacaccaaa tctcagtgga tccgtcattc cagtatcagc attgcccggc tggcgaaacc
     2821 ggttgactgg cagtcagaaa tgggcgaagt cgaactggtg atcatgctga cgctgggtgc
     2881 taacgaaggg atgaatcatg tgaaagtctt ctcgcagctg gcgcgtaaac tggtgaataa
     2941 aaacttccgc cagtcgttgt ttgccgcgca agatgcacaa agtatcctga cgctgctgga
     3001 aacagaatta accttctgac gttagccctg aaaacgggcg ctgtactctc ccggcgtcag
     3061 actaaactga cgccggaaaa cgcgacaaaa atagtcgcta tccggaaaac cgcaacgctg
     3121 tgccacttca tgaattgaca gatgatattt ctgcaaaatc atcctcgcct tcgccattcg
     3181 cacccaacgc acatactcga taaaacgcat cgttccatgc tgagcaaaca gttttgacag
     3241 atgattgggc gtgatattaa aaaactgcgc cacgctctcg cgggtgagcg gctgggcata
     3301 attatcctaa acccagttac aaatgctgtg atagagaaaa tcgccgcgcg gttgtgaatt
     3361 acccggcggc atattcacca cgttgcggca aagatgcagc aaacttaaca ccagtggctg
     3421 aatgatattc tgctccagcg gcgaacggct aagatgggtg agggcggtaa gcatcgcttc
     3481 accttcaccg cgttgcaggc tgggaagctg ggtctggcga gcaggatgca acaacgacgt
     3541 ggtgcggcta tcgtaaaacg ataacccaag ccaggtcggt gcgaacacca ggcttaacag
     3601 catcaccggt ttgttgttga tcggtaaatt agccgcgcgt gccggaataa acagcatttc
     3661 tccttccacc attcgctgct gctttgcttc cagcaggttg ccgtattcgc cgcgcagcac
     3721 gatatccaga cggggaaaat caatacataa actgcccacc ggtaacgagg caggctggct
     3781 ggcaaaccag acgcgcccca gtagttgcgg gttaagcact aggccgctga acagatcggc
     3841 gaaaaattgt tgatcggcgg gcaagcctgg tgctttcatg aaagttcccg attaaagatg
     3901 cattaactgg cgaaattctt tcactttgct gcggctgacc ggcacttcaa aatccagatc
     3961 tttcagtcgc agaatgtagg tgttattaaa ccacggttcg atttcgcgta ttttgttcag
     4021 attgacacaa aatgagcgat ggcagcggaa aaaatgcgac ggcggcagtt tgctgcaaaa
     4081 ttcggtaatg ttcatcggca ttacgtagga ttcacgccgc gtatagacaa aggtcatttt
     4141 ctcgtgcgct tcggcgtaat agatatcgtt aattggcgtg acgattattc gctcatcttt
     4201 caccagatta atggtgtcat tctcacgcgt taccgtcgcg gcaggcgtac tgctggtctg
     4261 ctgttgttgc caggccgctt ccagtttttg cagcatcccg gtaatacgtg actcctgata
     4321 cggtttgaga atgtagtcaa acgcctccag ttcaaacgct tctaccgcat gttctttcca
     4381 cgcggtgatg aacacaataa acggtttatg ggcgaactgg ctgatgtttt gcgccagcaa
     4441 cacgccatcc agcgacggaa tattgatatc cagaaaaatg gcgtcgacgc ggttatgctg
     4501 caaaaacttc aacacgtcca gaccgtcgtc aaaggtgccg acaatctcca tctggctgtg
     4561 ctctttaatt agccagctca gttcctgttg tgccaggaat tcgtcttcaa caatgatgac
     4621 tttcacaata tcactccggc tcaaagcaat aacgtagcct gtgaggcgac tggggtgcgt
     4681 tggttaggaa tgtaaaacgc aatttccgtc cccggctcca ggcggcggat atgcagcccc
     4741 tcgccataca ataacttcac gcgatgatgg acattcagca ggccaatttt attgcccggc
     4801 atttcattcg cttcgacccg ctcaatcacc tttggaacga tgccgtgccc ggtatctcgc
     4861 accgcaatgc gtacccgatt tccgcactct gcaacgctga tggtgacaac gcctttacct
     4921 ttgcaaggct gaataccgtg gacaatggcg ttctccacca acggctggat cagcaggctg
     4981 ggaatgcagc aattcacctc ttcatcaata tcatagataa ccgtcagctt gtcgccaaag
     5041 cgggcctgct caatggcaat atagtcttta atttgataca gctctttttt gatatcgatt
     5101 tgctcatcgt cttttaattc aatgttatag cgcagataac gcgacagatt aaagatcaac
     5161 tggcgagcgg tatccggatt cagacggatt gacgatgaaa tagcgttcag agcgttaaac
     5221 agaaaatggg gattaatttt gctttgcagg gcgcgaatct ctgccttatt tgccatttca
     5281 cgtagctgct cggcgcgtga aacctccagt tgcgtggaga tgatttgcga cagaccgacc
     5341 gccatttcct gtaatgacga ggtgatctga tgcgcgtggc agtagtaaat tttcagcgtt
     5401 ccggtcacga cccctttctc ccacaatggg atcaccagca tggaatgaat ctctggtgtg
     5461 cggtgggctt catcattgtt tttaatgatg atttttccgt aattcatcgc ctgacgggta
     5521 gtcgggctaa tgaagtcatc gccattctga tagttatgtt cacccacgcc aacataggcc
     5581 agcacatgat cggtattagt aatcgccacc gcatcggcgt gaatgtcatc gcggataatt
     5641 tcgcagacct tgcgtaatga ctcgctattg acatggcgaa acagcggtag cgttttgttg
     5701 gcgatatcca gcgccagcgc tgtgctttgg ggattttcag gtttatccag cctgatatac
     5761 aacccgccag aatgctggtg ataaagcagg ggatcgccgt cacgccgccg atatcaatta
     5821 aataccggtg aatacccgca atcacgccgg taatgatgcc tacccacggg ccaaacagaa
     5881 tcccgccgga catcaccgcg ataatacgta cgttcaccag cgagccttct acgggaacgc
     5941 cggaccaggt gctgaacagg gcgaacagcg agaaaatggc ggtaacggcg agcaattctt
     6001 ttggggagtg cgccgacttg tgcaacagtt cgcgaaacag gcggatacgg atgagaaaga
     6061 acaggcagat aagcattaac gctgcccgat cgaagaccgc cagcagcatg ttgaatattt
     6121 cgtgcacggg tgactctgaa taaggattaa acctctatga taggtaacct gaaggctgat
     6181 gaccagcagg ccgtttttga ggaaaaacca taaatttaaa aggggattaa aagataaatg
     6241 gtgtattagc aatgtatctc ttgataatgc acttctggct ggtaaattta ttgaaattat
     6301 aatgttaata aaattggtgg tgattttctt gttagcgtag aaaaatttcc tgtttttgaa
     6361 gtggagttcc tcatttaaat ccattgataa aaatgttcta aaacgctgga cagacagaaa
     6421 ttaatcaggc tattttcaaa acagccacca ttgtggcagc gtcgctcgga cggtccgggc
     6481 gctaacgtta atctgaggaa attatggctg acactcgccc tgaacgtcgc tttacgcgca
     6541 ttgatcgtct cccgccctac gtttttaaca tcaccgctga actgaaaatg gctgcgcgtc
     6601 ggcgcggcga agatattatc gatttcagca tgggtaaccc ggacggtgcg actccgccgc
     6661 atatcgtcga aaaattatgt actgtggccc agcgcccgga cacgcatggt tactccactt
     6721 cacgcggcat tccgcggtta cgtcgcgcca tttcccgctg gtatcaggat cgctacgacg
     6781 ttgaaatcga cccggaatca gaagccatcg tcactattgg ttcgaaagag ggcctggcgc
     6841 atctgatgct ggcgacgctg gatcatggtg acacggtgct ggtgccgaat ccaagttacc
     6901 cgatccatat ttatggcgcg gtgattgccg gggcgcaggt acgctcagtg ccgctggtgg
     6961 aaggtgtcga tttcttcaac gaactggagc gcgccattcg tgaaagttat ccgaaaccga
     7021 agatgatgat cctcggcttc ccgtcgaacc caaccgcgca atgcgtggaa ctggagttct
     7081 tcgaaaaggt tgtggcgctg gcgaaatgct acgatgtgct ggtggtccat gacctggcct
     7141 atgccgatat cgtctacgat ggctggaaag cgccgtcaat tatgcaggtg ccgggtgcac
     7201 gcgatgtggc ggtcgagttc tttacgctgt cgaaaagcta caacatggcg ggctggcgta
     7261 tcggctttat ggtcggcaac aaaacgctgg tcagtgccct ggcgcgaatc aaaagctatc
     7321 acgattacgg cacttttacg ccgttgcagg tggcggcgat tgcggcactc gagggcgatc
     7381 aacagtgcgt gcgcgacatt gccgaacagt acaaacgccg tcgtgatgta ttggttaaag
     7441 ggctgcatga agcgggctgg atggtcgaaa tgccgaaggc ttcgatgtat gtctgggcga
     7501 aaatcccgga accatatgcg gccatgggat cgctggaatt tgccaagaag ctccttaacg
     7561 aagcgaaggt ttgtgtctcg ccggggattg gctttggcga ctatggtgat acgcatgttc
     7621 gctttgcact gattgaaaac cacgaccgta ttcgtcaggc catcagaggg attaaagcga
     7681 tgttccgtgc cgacggttta ctacccgtca gcagcaaaca tattcacgaa aacgcggaat
     7741 aaacaccgtc gtcagaaatg caaacaggag cctgatggct cctgtttttc attgcacagt
     7801 ttgcttttag atcatcaggg caaaagtacc ggcccaaacg atgaccataa aagaaatgcc
     7861 cataaagaaa tatttcactt tacatctctc cgcgtaatta ctgttacgcc tgaatgactt
     7921 cattacacct gatacagtga gttgacaccg ggcaaagcga ccttaaaagc cctgtatttt
     7981 accgtgggca tcaccccaga attctgagct aaactgctcg agacggcgct aatgtacgcc
     8041 taaaactga
//
LOCUS       DAZXUK010000182         8035 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZXUK010000182 DAZXUK010000000
VERSION     DAZXUK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.182"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            4..741
                     /gene="ygaZ"
                     /locus_tag="ACH3L8_003071"
     CDS             4..741
                     /gene="ygaZ"
                     /locus_tag="ACH3L8_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFT4086083.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            731..1066
                     /gene="ygaH"
                     /locus_tag="ACH3L8_003072"
     CDS             731..1066
                     /gene="ygaH"
                     /locus_tag="ACH3L8_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFT4086084.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            1157..1687
                     /gene="emrR"
                     /locus_tag="ACH3L8_003073"
     CDS             1157..1687
                     /gene="emrR"
                     /locus_tag="ACH3L8_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFT4086085.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            1814..2986
                     /gene="emrA"
                     /locus_tag="ACH3L8_003074"
                     /pseudo
     CDS             1814..2986
                     /gene="emrA"
                     /locus_tag="ACH3L8_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            3003..4541
                     /gene="emrB"
                     /locus_tag="ACH3L8_003075"
     CDS             3003..4541
                     /gene="emrB"
                     /locus_tag="ACH3L8_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFT4086086.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(4605..5120)
                     /gene="luxS"
                     /locus_tag="ACH3L8_003076"
     CDS             complement(4605..5120)
                     /gene="luxS"
                     /locus_tag="ACH3L8_003076"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFT4086087.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(5270..6826)
                     /gene="gshA"
                     /locus_tag="ACH3L8_003077"
     CDS             complement(5270..6826)
                     /gene="gshA"
                     /locus_tag="ACH3L8_003077"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFT4086088.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(6899..7327)
                     /locus_tag="ACH3L8_003078"
     CDS             complement(6899..7327)
                     /locus_tag="ACH3L8_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFT4086089.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(7324..7890)
                     /gene="yqaB"
                     /locus_tag="ACH3L8_003079"
     CDS             complement(7324..7890)
                     /gene="yqaB"
                     /locus_tag="ACH3L8_003079"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFT4086090.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         1890 a         2129 c         2021 g         1995 t
ORIGIN      
        1 tctatggaaa gccctactcc acagcctgct cctggttcgg cgaccttcat ggaaggatgc
       61 aaagacagtt taccgattgt tattagttat attccggtgg cctttgcgtt cggtctgaat
      121 gcgacccgtc tgggattctc tcctctcgaa agcgtttttt tctcctgcat catttatgca
      181 ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg ggagtagttt gtgggttgct
      241 gcactgagcg tcatggcaat ggatgttcgc catgttttgt atggcccgtc actgcgtagc
      301 cgtattattc agcgtctgca aaaatcgaaa accgccctat gggcgtttgg cctgacggat
      361 gaggtttttg ccgccgccac cgcaaagctg gtacgcaata atcgccgctg gagcgagaac
      421 tggatgatcg gcattgcctt cagttcatgg tcatcgtggg tatttggtac ggtaataggg
      481 gcattctccg gcagcggctt gctgcaaggt tatcccgccg ttgaagctgc attaggtttt
      541 atgcttccgg cactctttat gagtttcctg ctcgcctctt tccagcgcaa acaatctctt
      601 tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa cgctattttc tattcccgtc
      661 gccattctgg caggcattgt ctgtggctgc ctcactgcgt taatccaggc attctggcaa
      721 ggagcgcccg atgagctatg aggttctgct gcttgggtta ctggttggcg cggcgaatta
      781 ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat gcccgcccaa ccaaacgtgg
      841 cgcggtaggt attttgctcg acaccattgg catcgcctcg atatgcgctc tgctggttgt
      901 ctctaccgca ccagaagtga tgcacgatac acgccgtttc gtgcccacgc tggtcggctt
      961 cgcggtactg ggtgccagtt tctataaaac acgcagcatt atcatcccaa cactgcttag
     1021 tgcgctggcc tatgggctcg cctggaaagt gatggcgatt atataaccca caagaatcat
     1081 ttttctaaaa caatacattt actttatttg tcactgtcgt tactatatcg gctgaaatta
     1141 atgaggtcat acccaaatgg atagttcgtt tacgcccatt gaacaaatgc taaaatttcg
     1201 cgccagccgc cacgaagatt ttccttatca ggagatcctt ctgactcgtc tttgcatgca
     1261 catgcaaagc aagctgctgg agaaccgcaa taaaatgctg aaggctcagg ggattaacga
     1321 gacgttgttt atggcgttga ttacgctgga gtctcaggaa aaccacagta ttcagccttc
     1381 tgaattaagt tgtgctcttg gatcatcccg taccaacgcg acgcgtattg ccgatgaact
     1441 ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac gatcgccgct gcctgcatct
     1501 gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt ttaccaccgc agcataactg
     1561 cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa gatcagctcg agcaaatcac
     1621 ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac ggtgtggttc tcgaagcgat
     1681 gagctaacgc gtcatctcgc tcaaaaatcc agatttataa aagaaaaaat gaccggccag
     1741 catcgcaaca tgctggcctt tttggcaagc aggtcggctc agccgatgag ttaagaagat
     1801 cgtggagaac aatatgagcg caaatgcgga gactcaaacc ccgcagcaac cggtaaagaa
     1861 gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg ctctttataa ttattgccgt
     1921 agcgataggg atttattggt ttttggtact gcgtcatttc gaagaaaccg atgacgcata
     1981 cgtggcaggg aatcagattc aaattatgtc tcaggtgtct ggcagcgtga cgaaagtctg
     2041 ggccgataac accgattttg taaaagaagg cgatgttctg gtcactctcg acccgacaga
     2101 tgctcgccag gcgtttgaaa aagccaaaac tgcactggct tccagcgttc gccaaaccca
     2161 ccagctgatg attaacagca aacagttgca ggcgaatatt gaggtgcaaa aaatcgccct
     2221 cgcgaaagca caaagcgact acaaccgccg tgtgccgctg ggcaatgcca acctgattgg
     2281 tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt gcccaggcgc aactggacgt
     2341 cgctattcaa caatacaatg ccaatcaggc gatgattctg gggactaaac tggaagatca
     2401 gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc tagctggcgc tggagcgtac
     2461 ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt gccgtgcaac cgggtgcgca
     2521 aattagccca acgacgccgc tgatggcggt cgttccagcc accaatatgt gggtggatgc
     2581 caactttaaa gagacgcaga ttgccaatat gcgcatcggt cagccggtca ccatcaccac
     2641 cgatatttac ggcgatgatg tgaaatacac cggtaaagtg gttggtctgg atatgggcac
     2701 aggtagcgcg ttctcactgc ttccggcgca aaatgcgacc ggtaactgga tcaaagtcgt
     2761 tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag ctggagcaat acccgctgcg
     2821 cattggtttg tccacgctgg tgagcgtcaa taccactaac cgtgacggtc aggtactggc
     2881 aaataaagta cgttcaacac cggtcgcggt aagtaccgcg cgtgaaatca gcctggcacc
     2941 tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct ggctaatcca gaggtgcgtg
     3001 tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt cattatgacg attgcgctgt
     3061 cactggcgac attcatgcag gtgctgaact ccaccattgc taacgtggcg atccccacta
     3121 tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg ggtaatcact tctttcgggg
     3181 tggcgaatgc catctcgatc ccgcttaccg gctggctggc aaagcgcgtc ggggaagtga
     3241 aactgttcct ttggtccacc atcgcctttg ctattgcgtc ttgggcgtgt ggtgtctcca
     3301 gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg gattgtcgcc gggccgttga
     3361 tcccgctttc gcaaagtcta ttgctgaata actacccgcc agccaaacgc tcgatcgcgc
     3421 tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg cggcccgatc ctcggcggtt
     3481 atatcagcga taattaccac tggggctgga tattcttcat caacgtgccg attggcgtgg
     3541 cggtggtgtt gatgacactg caaaccctgc gcggacgtga aacccgcacc gaacggcggc
     3601 ggattgatgc cgtggggctg gcactgctgg ttattggtat cggcagcctg cagattatgc
     3661 tcgaccgcgg taaagagctg gactgttttt catcacagga aattatcatt cttaccgtgg
     3721 tggcggtggt ggctatctgc ttcctgattg tctgggagct gaccgacgat aacccgatag
     3781 tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg ctgcttgtgt atcagcctcg
     3841 cgtatatgct ctacttcggc gctattgttc tgctgccgca gttgttgcag gaggtctacg
     3901 gttacacggc gacctgggca ggtttggcct ctgcgccggt agggattatt ccggtgattc
     3961 tgtcgccgat tatcggccgc ttcgcgcata aactggatat gcggcggctg gtaaccttca
     4021 gctttattat gtatgccgtc tgcttctact ggcgtgccta tacctttgaa ccaggtatgg
     4081 atttttgcgc gtcggcctgg ccgcagttta tccaggggtt tgcggtggtc tgcttcttta
     4141 tgccgctgac caccattacg ctgtctggtt tgccaccgga acgactggcg gcggcatcga
     4201 gcctctctaa ctttacgcga acgctggcgg ggtctatcgg cacgtcgata accacgacca
     4261 tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac tgagtcggta aacccgttca
     4321 acccgaatgc ccaggcgatg tacagtcaac tggaagggct tgggatgacg caacagcagg
     4381 catcaggctg gattgcccag cagatcacca atcaggggct gattatttcc gccaatgaga
     4441 tcttctggat gtcagccggg atattcctcg tactgctggg gctggtgtgg tttgctaaac
     4501 cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta agtacaacta agccagttca
     4561 tttgaactgg cttttttcaa ttaattgtga agatagttta ctgactagat gtgcagttcc
     4621 tgcaacttct ctttcggcag tgccagttct tcgttgctgt tgatgcgtac ctcacgttcc
     4681 agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt gcatctggta agtgccacac
     4741 tggtagacgt tcagttccgg gatctgattc tgatcctgca ctttcagcac gtcttccatt
     4801 gccgctttcc aggcatcagc aacacgctgc tcatctggcg taccaatcag actcatataa
     4861 aaaccggtgc ggcagcccat tggcgagata tcgataatct ctacaccatt accgttaaga
     4921 tggttacgca taaaaccagc aaacaggtgc tccagggtat ggatccctct ttctggcatc
     4981 acttctttgt tcggcacgca gaagcgcaga tcgaacacgg tgattgcgtc gccatgcggg
     5041 gtgttcattg ttttcgccac ccgaactgca ggcgcttcca tccgggtatg atcgactgtg
     5101 aagctatcta acaacggcat ttagccacct ccggtaattt ttttaaaaat tttctgaact
     5161 ctttcttccc aggcgagtct gagtatatga aagacgcgca tttgttatca tcatccctga
     5221 attcagagat gaaattttgg ccactcccga gtggcctttt tcttttctgt caggcgtgtt
     5281 tttccagcca caccgcaaac ggttcggtat cagcggtttc catttcctgc tgacggcgtt
     5341 cagatgcctc gcgctcggct acaaaatcct cttcgcgcag aatttccagc ggctcttcac
     5401 gcagcagatt acggtaggct tctgcaaacg ctttgcctgt tccgccaata ccagtatcaa
     5461 tcatagacct taagatacgg gcagagaaag tcagatcggg attatcgaag caggcaacca
     5521 gttcatcaca cactttctga tacgcttcgc cgccgttaat gctatccagc gtttgcgcga
     5581 cgcgtttcag atcgcggaac agatctttac ccacctgcag taacgggaac tgcgcggttt
     5641 cgcagccgat acccagcgtc aggcccggtt tgcgaccttc aaggatcacc cggttccagt
     5701 taacgcgcgt acaggcaagt tcgctactgc tcatttccgg tgcatcagcc agcgcacacc
     5761 agaccataaa caggtcgagg aatcgcacct gctgttcatc tacaccaatc ggcgagaacg
     5821 ggttgatgtc cagcgaacgc acttcaatat attcaatgcc gccacgtaac agcgcatcag
     5881 aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat cggcgcatac agttcgtttt
     5941 caatctgcag cacgttgcta ttgatttgca gcctcttacc gtctttctca ataccaatct
     6001 tcgcgtactc ttccgatggc gttttgattg cctgtttaag gcccgctacg tactcgtaaa
     6061 gatcgttgaa ggtaatacca agattgcttt gcgatttatt ggtatagccg agatcgctca
     6121 aacgaagaga ggtcgcatac ggcaggtaat acataccgca ctcggttttc tcaaacggca
     6181 gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc cggagatgca ccaaacagat
     6241 aaggaataac ccaaccgaaa cgatagtaat tgcggataac gcggaaatag cccgcagaaa
     6301 ttttctcttt ggcatcagcg cccgagatat caccgcactt cgcttgccag aatgccattg
     6361 gcaaagagaa attgtagtgc acgccggaaa tggtttgcat cagcgcgccg tagcgatttt
     6421 tcagcccttc acgatacagc gttttaaagc gtccggtgtt agaagtgccg tactgtgcca
     6481 gttcgatgtc ctgaccttct gcgatgtagc atggcatact taacggccac atccgctcat
     6541 cgcccatatt gcgcgccgta taacgatgca gatcgcgcat aaaggtcagc atatgttcaa
     6601 tatcaccatc cactggtgta atgaattcca gcaatgcttc cgcaaaatcg gtagtaatcc
     6661 atttgtgcgt cagtgcggaa cctaatgctt caggatgacc tgttgttgcc agtgtgccat
     6721 cagcattaac acgcaaagtt tcgcgctcca gcccacgctg tatccccttt aacgcctgag
     6781 gatgtttttc cagccaggcc agcgcctgtg atacgtccgg gatcaaattg acctcccgcc
     6841 tgtcaaaatc gttttaatta gcataactgt aatggtgacc atatgtgcag gcctacaatt
     6901 agtgccacca catcatgccc tgaacggtcg ctgctgcaac tgcaacatag cgtaacgctt
     6961 taccaaggca caaaaaaaag attactggcc cccaggagat gcgcatccat cccgctaaca
     7021 gacacagtaa atcgccaacc acgggcatcc agcttaataa tagcgtgact gcaccatagc
     7081 gtttcagcca gccggtagct ttctcttgcc agcgcgatgt tttacgcaat ggaaagaaac
     7141 gcccaaggat aacgttagtt aaccctccaa ggctattacc cattgttgct gttaaaacta
     7201 aaacccaggg atgactgatc ccggaaagca acattgccac cagcacgact tcggagttgc
     7261 cgggtaatag tgtagcgctg agaaaactac tggcaaacaa cgagaaaagc gataacgctt
     7321 cactcacagc aagcgaacat ccacagcgtc catgcctgct gcacgggccg cctgaatacc
     7381 gaaatcggca tcttcaaaga ccacacactg cgtcggttgc acgcccatac gctgcgcgca
     7441 caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg tgatcagcag cgacgacggc
     7501 gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc tcagcgatgg cgctttcact
     7561 ccccgttcct acagccattg ggcgacgacc atgccaactt ttcaccactt caacaagagg
     7621 aagcggctcg acactatcca gcagcatact tcttactgct tctgtttttt cacgcgctaa
     7681 cgcatgcggg tcgagatcgg cctgattcag ctcaataatt gcctgagcaa tacgccaggt
     7741 gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac tgaagaccgt agtgccctaa
     7801 tacttcgcgc cacgctttac ggtgcgtagg cgccgtatcc aggattgtgc catccatatc
     7861 aaaaattaaa cctgcataac gctcgtacat ggtcttctcg caagtcgaaa aatatgacgc
     7921 tactttagcg taattgcttg attttgtcgc tgatggcgaa ggagaagaaa attcaggaaa
     7981 tggataaagt agttatttag aaatgagata tttttgaagg aaattttttg gagaa
//
LOCUS       DAZXUK010000183         7998 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZXUK010000183 DAZXUK010000000
VERSION     DAZXUK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.183"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACH3L8_003080"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACH3L8_003080"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFT4086091.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACH3L8_003081"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACH3L8_003081"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFT4086092.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACH3L8_003082"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACH3L8_003082"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFT4086093.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="ACH3L8_003083"
     CDS             6038..6658
                     /locus_tag="ACH3L8_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFT4086094.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="ACH3L8_003084"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="ACH3L8_003084"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFT4086095.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
BASE COUNT         1811 a         2332 c         2034 g         1821 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcc
//
LOCUS       DAZXUK010000184         7981 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZXUK010000184 DAZXUK010000000
VERSION     DAZXUK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7981
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.184"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(168..566)
                     /locus_tag="ACH3L8_003085"
     CDS             complement(168..566)
                     /locus_tag="ACH3L8_003085"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFT4086096.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="ACH3L8_003086"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="ACH3L8_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFT4086097.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..6128
                     /gene="traI"
                     /locus_tag="ACH3L8_003087"
                     /pseudo
     CDS             875..6128
                     /gene="traI"
                     /locus_tag="ACH3L8_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6148..6894
                     /gene="traX"
                     /locus_tag="ACH3L8_003088"
     CDS             6148..6894
                     /gene="traX"
                     /locus_tag="ACH3L8_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFT4086098.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6949..7509
                     /locus_tag="ACH3L8_003089"
     CDS             6949..7509
                     /locus_tag="ACH3L8_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFT4086099.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7640..7852
                     /locus_tag="ACH3L8_003090"
     CDS             7640..7852
                     /locus_tag="ACH3L8_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFT4086100.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1814 a         2029 c         2579 g         1559 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggcagca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gcataaactt cagcatcaga atgactccct ttcttgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaacgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gctgtccgtc aggtggaggc cagtgcctcc acctgacgga cggacagtca
     1261 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat
     1321 caggaaccac agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa
     1381 tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct
     1441 aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg
     1501 ggctatgaaa ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg
     1561 gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg
     1621 aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa
     1681 gtcagaatgg ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1741 cgtgacgcag cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag
     1801 gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa
     1861 gtgcagttta tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat
     1921 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1981 cccctcgatc gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca
     2041 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     2101 agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg
     2161 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     2221 ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     2281 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     2341 cagttgctgg aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa
     2401 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     2461 gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag
     2521 gatgccgggg tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc
     2581 agtgaaccgg accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag
     2641 aagagagcgt ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc
     2701 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt
     2761 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg
     2821 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga
     2881 cggcgcagag tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta
     2941 tttcttccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     3001 gtgagcgtct gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     3061 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac
     3121 cggccaccct gcctgtgagc gattcaccgt tcactgcact gaagctggag aacggctggg
     3181 tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga
     3241 tggcaatgga caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt
     3301 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     3361 tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc
     3421 tgcagaaagc cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc
     3481 tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     3541 ctgcagaagg aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg
     3601 gtgatttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     3661 cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca
     3721 cgccgctgat ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg
     3781 ccgccacccg tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg
     3841 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga
     3901 gtgaacgtcc ccgtgtcgtg gggctggggc ccacacaccg tgcggtcggg gagatgcgca
     3961 gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc
     4021 gcagcggaga aactccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg
     4081 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg
     4141 tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     4201 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg
     4261 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc
     4321 ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact
     4381 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     4441 tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     4501 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     4561 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg
     4621 ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc
     4681 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc
     4741 accggattgc cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta
     4801 acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg
     4861 acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg
     4921 gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt
     4981 ctgacggtca gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg
     5041 acctggccta tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg
     5101 cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg
     5161 ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg
     5221 atgccatcaa caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg
     5281 accgggaggt catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg
     5341 cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt
     5401 ttattgctcc gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta
     5461 acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc
     5521 ggggattcag cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc
     5581 agggcagccg taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg
     5641 cccgtgataa tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga
     5701 atcccggtgc catcaccggt ggccgcgtgt ggggggatat cccggacagc agcgtccagc
     5761 cgggagccgg aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag
     5821 aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga
     5881 acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg
     5941 agcgtgaccg ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg
     6001 aaccacaacg ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc
     6061 gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg
     6121 gagactgata caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac
     6181 gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga
     6241 cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg
     6301 atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt
     6361 gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac
     6421 cggctgtggg gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg
     6481 tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa
     6541 acgcgcagtg ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg
     6601 tccggcacca gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac
     6661 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     6721 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     6781 accgttggtc tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt
     6841 ttcccgacgt tctatgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     6901 tggtatcttt ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag
     6961 aagcgaccgg tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg
     7021 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     7081 gagaaagcgg cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg
     7141 tccatttatc tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg
     7201 ccgggattat ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta
     7261 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     7321 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     7381 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcgggagcg
     7441 cgtctggcga aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     7501 gagaaataaa aaaagcctcc cccagagaga ggaggcaggg aaataaggct taaaaggaaa
     7561 taacttccgc ttccgaaagg aagccagtcc actgaattat ttatcacaat aatactgaca
     7621 actaaagggg gacaagaaaa tgaatggatt cagaaacagt tcacggaacg gccaggtctg
     7681 gcgttaccag cgtgccggcg ggcgggccgt tattctggaa gtcagtggac gctggatgga
     7741 agcggcagaa gcatggcgac gggctgcctg tgttgcgcct aggacagact ggcagcagtt
     7801 tgcccggaaa agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa
     7861 gcagccttca ttactctagg agtcagaagg ctgaaaggag tttatgagat ttgcttctta
     7921 atatccggtc gggcagtatg accggacagt tctgagtagc acagagtctt ttccgcatta
     7981 a
//
LOCUS       DAZXUK010000185         7958 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZXUK010000185 DAZXUK010000000
VERSION     DAZXUK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.185"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(195..680)
                     /gene="sixA"
                     /locus_tag="ACH3L8_003091"
     CDS             complement(195..680)
                     /gene="sixA"
                     /locus_tag="ACH3L8_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFT4086101.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(883..3027)
                     /gene="fadJ"
                     /locus_tag="ACH3L8_003092"
     CDS             complement(883..3027)
                     /gene="fadJ"
                     /locus_tag="ACH3L8_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFT4086102.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3027..4337)
                     /gene="fadI"
                     /locus_tag="ACH3L8_003093"
     CDS             complement(3027..4337)
                     /gene="fadI"
                     /locus_tag="ACH3L8_003093"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFT4086103.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4517..4801)
                     /locus_tag="ACH3L8_003094"
     CDS             complement(4517..4801)
                     /locus_tag="ACH3L8_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFT4086104.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5173..6513
                     /gene="fadL"
                     /locus_tag="ACH3L8_003095"
     CDS             5173..6513
                     /gene="fadL"
                     /locus_tag="ACH3L8_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFT4086105.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6879..7937
                     /gene="yfdF"
                     /locus_tag="ACH3L8_003096"
     CDS             6879..7937
                     /gene="yfdF"
                     /locus_tag="ACH3L8_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFT4086106.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
BASE COUNT         2137 a         2013 c         1813 g         1995 t
ORIGIN      
        1 atattattca tttcaacatg taactttggg caaaatcaag atacgcgtag aagaataatt
       61 tccgaatgaa tgtagaaagt gttttattta tagataagga gagtcttcga tagtagtgag
      121 gttgacagat atccgctgcc taatgagcaa ttatgcccaa tgaatctacc tcattgggca
      181 taatttggaa cacatcagat agcttttgcc atcttcagat tacacgggct catctgccag
      241 ttgaatgtac cgtttccgct ttcatcaagc gttacgctgg caatggccga ggtggtaaac
      301 ataggcggcg tttcgcccgg acataactcg gcaaccagat acccgactaa cggtaagtga
      361 gagatcacca gtaccgaagc aacaccttca ttggtcaacg cctgcaaata ggcactgacc
      421 agaccaacat cgccgcaggg cgttaactcc ggcagaactt ctgcactgga aggcaggttc
      481 agacaatccc ctacttcttc cagtgtttgc tcggctcgca ggaacgggct caccagaacg
      541 cgttcgattt ccactttttg acctttcagc cagttcgcca tcaggcgaga ttcgtcacaa
      601 ccattagtgg tcagaggacg aaccgaatca ctggcggaat cgagggctgc gtcgccgtga
      661 cgcatgataa aaacttgcat attgcaccgc ttttgttaac cagattcgcc cgttttcttt
      721 catctgaaac cagagaaaga aaacggtggc cggcattgtg ccttatccat tcaccgaatg
      781 aaacgctgtt ttttacctca atggcgtaag tatagtcaat atgcgtttac attttgacca
      841 acactccgcc attcagcgcg gattcatata gctttgacct tcttattgca ggtcagttgc
      901 agttgttttc caaaaacttt ccccacgcgc gcccatctcg accaaacgct cgcaaggggt
      961 aaaacgggaa ccatactgcg tggccagtcg ttgcattatt gcaaccactt cgcccgcgcc
     1021 gagagaatcg atatagcgga acggtccacc gagaaatggc ggaaaaccaa tgccaaatac
     1081 cgcaccaata tccccgtcac gcacgctacg gataacctgc tcatcaacac aacgtactgc
     1141 ttcattcagc atcaacatca cacaccgttc agcaacctgc ggtgcggaga gtcgcccctg
     1201 cccttgtgcg ccaatgagcg ggtaaatggc aggatcgacc tgttttttgc ttttacgccc
     1261 tttctgacca taaagataga aaccccgacc attttttctg cctttgcgat cgtcgttcaa
     1321 aattgaagaa acaacatttg caggcgcgct aaaacgttct ccataagcgg cttccagtac
     1381 aggaataatt ttagtcccgg tgtcgattcc tacctcatcc aaaagttgga ttgggcctac
     1441 cggaaaacca aatttcacta gcgcggcatc aatgtgctct acccgttcac cttcggtcag
     1501 catgcggata gcttcattaa tgtaaggcgc taagatgcga ttgacgtaaa acccggcttt
     1561 gtcacgcacg acaatgggcg ttttgccctg ttttttcgcc agttttaccg tagtggcgat
     1621 ggtttgcgcc gatgtacccg catgaggaat aatctctacc agcggcattt tttccaccgg
     1681 actgaagaaa tgcaggccaa taacttgctc aggtcgcgcg gcgtgagcgg cgatatcacc
     1741 aatcggtaaa gatgacgtgt tcgaagcaaa aatggtatga gcggcgcaat tttgctccac
     1801 ttccgccacc atctgttgtt ttaattcgag attttcaaac accgcttcaa taatcagatc
     1861 gcgatgggca aagccgcgat agtccgtcgt tccggagatt aatgccagct gtttgtcacg
     1921 ttcgctggct ttgagatgac gacggcgaac tttgccctcc agctgatccc aactgtactt
     1981 cagcgcatga tttatgccct gcgggttgat atctttaatt ctgaccggaa gccccgcttt
     2041 acaagcagtg acataagcaa taccgccgcc catcaagcca ccacctaaaa ttcccacgct
     2101 gtttaatggc gcaggcggtg catcactacc gggatctttc ttcacgtccg tactggcaaa
     2161 aaagatacta cgcaaagctt gcgattgcgg cgtcatcgcc agttcgccaa aggcgcgagc
     2221 ttcagcgtca tagccgctgc tggtgccctg cgctaatccc gtttcaacaa cctccaggat
     2281 gcgttctgtc gccggataat tgccttgagt tttgtgttct gttttcttgc cgaccatttt
     2341 gaacagcagc gcacgaccta acggccccgc cagaatacgc tcgcgtacag gtagagggcg
     2401 ggaagatggg cgatccttct tcgccagctc aacagcggct tccagcaaaa tggagtgcgg
     2461 aacaacgtca tccaccagcc ccagctttaa tgcctgtttc gcccgaagtt gttttccggt
     2521 gagaatcatc tctaatgctg tactgacgcc tatcagacgc ggtaaacgct gggtgccgcc
     2581 tgaaccgggt aacaatccaa gttgtacttc aggcaaaccg agcaccgttt taggatcgtc
     2641 agtacaaacg cgaccgtggc acgccagcgc cagctccagc ccaccgccca ggcaagcgcc
     2701 atgaatagcc gcgataaccg gaatgggcaa agcatgaatc tccgccatca actgttggcc
     2761 ctgccgcgcc agtgcttccg cttcttgcgc cgttttgcag ttgccaatca tgttgatgtc
     2821 tgctccggcg ataaaattgt ccggtttcgc ggagataaac accacgccgc gcaactcttt
     2881 gttttcacgg agttgcttaa taatggcgcg cacctgcgag gcaaattccg ccttcagggt
     2941 attcattttc tcatccggta cgtcgatggt gatgatagca atgttgtcca gacgaacatt
     3001 aagagtaaac gctgatgcca tttccattat tccgcctcca gaaccattgc cgcgccaagc
     3061 ccaccggcag cacaggcggt aactaaacca aatccaccgc cgcgacggcg aagttcatgc
     3121 aatgtctggg taatcatccg cgcgccggtc gccgcgaagg gatgtccgta agcaatcgaa
     3181 ccgccaagca cgttaaattt gctatcgtcc acttcgccag tggcatgtgc acgccccagt
     3241 acttcacgag caaaacgttc actacccagc aactgaatat tcgccagtgt ctgagctgca
     3301 aaggcttcgt gcatatcgat caatgtcagt tcgctcatcg tcaaaccggc acgttccagc
     3361 gccagcggtg ttgaccaggc tggaccgagc aacatgtcct gccagacatc aatcgcagta
     3421 aatgcgtagc tgcgcagata ccccagcggc accagcccta attctttcgc ccgggattca
     3481 gtcatcagga tcaccgctgc cgcgccatcg gtcagcggcg tactgtttgc cgccgttacc
     3541 gttccgtgtt tgcgatcaaa cgccgggcgc agctttgcgt aatcggcaag cgaggaatta
     3601 ccgcgaatat tgttgtcttc gacaagcggt tgtttataag gagggataaa ggcagtcatc
     3661 acctcttctt tgagttttcc gtctgaccat gcctgagcgg cacgctgatg cgaacggtgc
     3721 gctaatgcat cttgctgttc tcgggtgatg ccgtaggttt tcgccatttg ttcggcggtg
     3781 tcgcccatcc gcaagccagt agaatattct gctaccgcag gcggtacggg cattaagtcg
     3841 cgcaaacgca ggcgagagaa gagtttcagg cgctggctca tggttcgagc tttgttgaca
     3901 tcaaccagca cgtacgccag ttttttactg acgccaattg gcaatacgga agaggaatct
     3961 gccccaccgg caatccccgc tcggatagtt cccgccatca ggctttctgc gacgtttgca
     4021 accgcttgga aactggtagc gcaagcgcgg ctgacgctgt aagcatcggt atgcacattc
     4081 attccggtac cgagaacaat ttcacgcgca atgttggggg cttcaggcat ttgtacgacc
     4141 tgaccaaaga ccagctgttc aatcacttca gcggggatct cgctgcgtgc cagcagttcg
     4201 cctaccacca tcttccctaa atcaaccgtg ggaatgccat gaaaagccgt cgcctggcgg
     4261 gcaaaaggcg tacgtaaacc gctaacaatg gcgatacgat cgccctggcg ggtaaccagt
     4321 ggtaaaacct gacccataac actcccctgt aaaaaaataa ataaagtggt ctgacctgat
     4381 catagtctta accatttttt tacatttagc caagcggaga aaaaggaaag tgggagctat
     4441 gacacagtga agacagaaga aagaaaagaa aacgcccctg ccagcaactg acaggggcgt
     4501 acgtacccga gaggaattaa cgcagaccca gctggaagat cagcatttca gcttcgcagg
     4561 caaaagtgaa atcgatatcc aggcgcacac cgtcagactc ttcagtgaaa gtcggggaga
     4621 ttttgcacgg ttcggattcc acgctacggg atttttcagt cagcgccgcc agcgtttgtt
     4681 ctgcttctgc gcggtttgca aacacgcggc tgtaagacgc ggtgcagtcg gagttgtcca
     4741 taatggtgcc aacatccata cagcagcaaa ccggggtttc atcagcacta catttactca
     4801 tcgttgattt cctctgtatg tgcacccaag gtgccagata aacgttgtgg atattttacg
     4861 cttccggaaa gtgctgctcc agttgttaat tctgcaaaat cggataagtg accgaaatca
     4921 cacttaaaaa tgatctaaaa caaaattcac ccgaatccgc gagtgcgcaa cctccaaatt
     4981 ttgccagctg gatcgcgttt cttagatcat atttgaaaaa agatggaaac atacttgcaa
     5041 cattctagct ggtccgacct atactctcgc cactggtctg atttctaaga tgtacctcag
     5101 accctacact tcgcgctcct gttacagcac gtaacatagt ttgtataaaa ataaatcatt
     5161 gaggttatgg tcatgagcca gaaaaccctg tttacaaagt ctgctctcgc agtcgcagtg
     5221 gcacttatct ccacccaggc ctggtcggca ggctttcagt taaacgaatt ttcttcctct
     5281 ggcctgggcc gggcttattc aggggaaggc gcaattgccg atgatgcagg taacgttagc
     5341 cgtaaccccg cattgattac catgtttgac cgcccgacat tttctgcggg tgcggtttat
     5401 attgacccgg atgtgaatat cagcggaacg tctccgtctg gtcgtagcct gaaagccgat
     5461 aacatcgcgc ctacggcatg ggttccgaac atgcactttg ttgcaccgat taacgaccaa
     5521 tttggttggg gcgcttctat tacctctaac tatggtctgg caacagagtt taatgatact
     5581 tatgcaggcg gctctgtcgg gggtacaact gaccttgaaa ccatgaacct gaacttaagc
     5641 ggtgcgtatc gcttaaataa tgcatggagc tttggtcttg gtttcaacgc catctacgct
     5701 cgcgcgaaaa ttgaacgttt cgcaggcgat ctggggcagt tggttgctgg tcagattatg
     5761 caatctcctg ccgggcaaac tcctcaaggg caagcattgg cagctaccgc caacggtatc
     5821 gacagtaata ccaaaatcgc tcatctgaac ggtaaccagt ggggctttgg ctggaacgcc
     5881 ggaatcctgt atgaactgga taaaaataac cgctatgcac tgacctaccg ttctgaagtg
     5941 aaaattgact tcaaaggtaa ctacagcagc gatcttaatc gtgcgtttaa taactacggt
     6001 ttgccaattc ctaccgcgac aggtggcgca acgcaatcgg gttatctgac gctgaacctg
     6061 cctgaaatgt gggaagtgtc aggttataac cgtgttgatc cacagtgggc gattcactat
     6121 agcctggctt acaccagctg gagtcagttc cagcagctga aagcgacctc aaccagtggc
     6181 gacacgctgt tccagaaaca tgaaggcttt aaagatgctt accgcatcgc gttgggtacc
     6241 acttattact acgatgataa ctggaccttc cgtaccggta tcgcctttga tgacagccca
     6301 gttccggctc agaatcgttc tatctccatt ccggaccagg accgtttctg gctgagtgca
     6361 ggtacgactt acgcatttaa taaagatgct tcagtcgacg ttggtgtttc ttatatgcac
     6421 ggtcagagcg tgaaaattaa cgaaggccca taccagttcg agtctgaagg taaagcctgg
     6481 ctgttcggta ctaactttaa ctacgcgttc tgataacgcg ttcgcctgga taaagtcacc
     6541 tgctatgcag gtgactttaa ctcccccact tcaccgaaag tagtttcctc attgcttgcc
     6601 tcctgagttt tgcaaacagc ctgatggcag cttgcccttt caaatcagta agtggtatcc
     6661 acagacaaac ctgaagggaa atgcatattt tcaagcgttc tgctcgtcct tctcaaagag
     6721 tttacttttc tgcatttcca ggatactcaa cccccctggc tattgtgcgc ttatacactc
     6781 aaattaaaga taggttctaa ataaatgagc gttttttgat agtctatttc attaggtaat
     6841 atatatttgt aacaaatcaa tcaacatgga ataaaatcat gctaccatct atttcaatca
     6901 acaataccag cgcagcttac ccagaatcca tcaatgaaaa taacaatgat gaaattaatg
     6961 gattagtaca agagttcaaa aaccttttta atggtaagga aggaataagc acctgtatta
     7021 aacatctact tgagcttata aaaaacgcca tacgagtaaa cgacgatcct tatagatcta
     7081 atattaataa ttcctcagtt acttatattg atattggctc caatgataca gaccatatta
     7141 ctattggtat cgacaaccaa gaaccaatag aattacctgc gaactataaa gacaaagaac
     7201 tcgtccgtac tatcattaat gacaacatag ttgagaagac tcatgatatc aataacaagg
     7261 aaatgatctt cagcgcatta aaagaaatat atgatggaga tcctggtttt atcttcgata
     7321 agatatcaca caaactcaga catacggtaa cggaatttga tgagagcggg aaaagcgaac
     7381 caacggactt atttacctgg tacggtaaag ataaaaaagg cgactctctc gctattgtaa
     7441 ttaaaaataa aaacggaaat gattacttat ctctcggtta ctacgatcag gacgactacc
     7501 acattcaaag aggaattcgt attaatggtg atagtctcac ccaatattgt agtgaaaacg
     7561 ccaggagtgc ttcagcgtgg tttgaaagca gtaaagctat catggcagaa tcatttgcaa
     7621 ctggttccga tcatcaggtt gtaaacgagc tcaacgggga aagactgaga gaacctaacg
     7681 aagtttttaa acgtttaggt cgagcaataa gatataattt tcaagtggac gatgcaaaat
     7741 ttagacgcga taatgtaaaa gaaataattt ctactttatt cgctaacaaa gttgacgttg
     7801 accatcctga aaataaatac aaagacttta agaatctcga agataaagtt gaaaaaagac
     7861 ttcaaaatcg ccagacgaaa tatcaaaatg aaattaatca actatctgca ctaggtgtta
     7921 attttgatga tatttaaaat tatttttcca gttttttg
//
LOCUS       DAZXUK010000186         7940 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZXUK010000186 DAZXUK010000000
VERSION     DAZXUK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.186"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(52..531)
                     /gene="xdhC"
                     /locus_tag="ACH3L8_003097"
     CDS             complement(52..531)
                     /gene="xdhC"
                     /locus_tag="ACH3L8_003097"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFT4086107.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="ACH3L8_003098"
     CDS             complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="ACH3L8_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFT4086108.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="ACH3L8_003099"
     CDS             complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="ACH3L8_003099"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFT4086109.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4129..4884
                     /gene="actS"
                     /locus_tag="ACH3L8_003100"
     CDS             4129..4884
                     /gene="actS"
                     /locus_tag="ACH3L8_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFT4086110.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4963..5036
                     /locus_tag="ACH3L8_003101"
     tRNA            4963..5036
                     /locus_tag="ACH3L8_003101"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4995..4997,aa:Gly,seq:ccc)
     gene            5171..6787
                     /locus_tag="ACH3L8_003102"
     CDS             5171..6787
                     /locus_tag="ACH3L8_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4086111.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKGCQDRLKGDSSGLLSYLE
                     PETSSLRPSVSLSVSSEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEILDYLDIQDVGKATRADMLRVREVLQLLPKNRKQRFKTMPLSDLLNRESKNDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARGAFSPEQVRQLLV
                     AAKAYSQKTAGKPYHYYVTALAAVTGARLNEVAQLQVKDIRVTEAGTVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLIPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGVDGDTV"
     gene            6771..7427
                     /locus_tag="ACH3L8_003103"
     CDS             6771..7427
                     /locus_tag="ACH3L8_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFT4086112.1"
                     /translation="MAIPFEAPEFEKEAFNCPYCNAFAHQKWQQLYYYYSSTGGLPVH
                     TSTCEHCEEKSFWFQRRLLIPFASTAELPNPDMPDNCKADYMEARSIVGLSPKGAAAL
                     LRLCLQKLMVHLGEPGNNINADIRSLVQKGLPVRIQQAADICRIVGNQAVHPGEISLD
                     DDPQLAHGLFKLLNIIVDDRITRPKEIEAMFQSMPEGPRQGIENQDRQAREQQQAANE
                     "
BASE COUNT         1987 a         1984 c         2046 g         1923 t
ORIGIN      
        1 ttaaacacat aacctcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt
       61 tttctcgcaa tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt
      121 tcccgccagt ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag
      181 cattgccgtg gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgtgccgga
      241 tttcgcataa gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt
      301 gcggatctct tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc
      361 gtcgaccaac accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag
      421 cagtccttgt tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa
      481 aggcatcccg ttaatggtgc attcggtggt aattgtttcg ctgtgattca ttgcaatttt
      541 ccccccgccg cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc
      601 agacgaaact ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat
      661 tcgctgatag cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca
      721 tgttggcagc gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa
      781 ttgccgttat ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg
      841 cgcatggcat atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga
      901 aaggcgacga ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca
      961 ttaatcggga cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt
     1021 agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta
     1081 gcgacgttac ggatctgcgg tccagcaatg aacgtggccg cagcacataa cgccgggaga
     1141 tgacgttgag ttataggatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt
     1201 agcgagccat cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca
     1261 atatggcgat aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg
     1321 agcagtttgg cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg
     1381 cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa
     1441 ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat
     1501 cttcaccgcg ttacgaatag cagcggcaac aggaattatg ggtggctcac ccagtgactt
     1561 atgtccgtat gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag
     1621 atccggcatg gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt
     1681 agcatcgatg atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg
     1741 tacctgacct tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat
     1801 gcggttgatg gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc
     1861 aaacgccggt gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg
     1921 ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg
     1981 tcgttcaacc agcacgatat ggccttttat cagggtcaaa ttcatcgctg actgatgtag
     2041 catgactgcg gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc
     2101 aggcgcggca acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc
     2161 ggtatcttgt gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac
     2221 catttgcgag aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt
     2281 gatggttcca tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca
     2341 ggtgttagag gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg
     2401 gttctggcat tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca
     2461 ctccggcaac cctgcgctgc aaatacgttt gcccgtgagc ggattagcat ctccttcgcg
     2521 ggcggcgttg cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc
     2581 aagcatagac tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc
     2641 cgaggggagg ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag
     2701 gtaagcgact ttattccccc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc
     2761 ggtgttagac agaacatcca gactataacc tttcaaggtt ccgtcgcggt tcacgcccat
     2821 ttgcccgtca atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct
     2881 aagggaaact ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc
     2941 ttccagtaca tcctgtttat taccaaaacc gccaccgaca aacggtttga tgactcgtac
     3001 gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg
     3061 ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct
     3121 ttccatgtga caatgttgaa taacgggagt ctgatagtgc ccctgtacct ggtagtcggc
     3181 ggcatcgatt gtttgttgga cattgcccgt cgacatcgtg ctttgtttca gtaaattgcc
     3241 gccgttatgg attggtgcag cgtcttctgt cagcgccgct tctggcgtgg tgataacggg
     3301 taattcttgc cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg
     3361 ggccacgacg atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg
     3421 atcggcggta tcgcgcttgt tttcgtcaag tgtccaggca tgcccagctg tagcgaatgg
     3481 aatatcaggc acatcttccc aagtaaaaat cgccagtacg cccggtaaac ttctggcttg
     3541 ttcatcatta atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcgta
     3601 acacatgccc gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat
     3661 ggcatcgacg cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat
     3721 cccctcgatt tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta
     3781 cgccagaaac gacgataagc gttaggggag gaaggaagtg tgatgacgtt aaaaaaacac
     3841 agatatgata cgaaacagga ctctgaatag ggacagtgat acgtatcaat atgagtggcg
     3901 ataaaatcaa tgtgataaat caggattatt acgcatcagg atgggaaata gggtaaaaag
     3961 tcaggaactt cgatacctct acagcgaata ttttgctgat gatacttccc tttgccaaat
     4021 gaaattaacc tctttctcta ttaccacgtt tttccagaag caagagattg ggtatcaaga
     4081 ggctggctgc tatgatatgg cgtcttgttt tttaagcgag gaaagatttt gagtgcggga
     4141 cgcctgaata aaaaatctct gggtatcgtg atgtttttat cggttggact gctattggcg
     4201 ggctgttcgg gtagcaaatc atccgataca ggaacgtatt ccggctccgt ttacaccgtg
     4261 aaacgggggg atacgctata tcgtatttcg cgcaccacgg gaaccagcgt aaaagagctg
     4321 gcgcgactga acggcatttc ccccccttac accattgaag ttggtcagaa actaaaactg
     4381 ggtggggcga aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac gactaaaacc
     4441 gcatcggtta caccgtcatc agcggtaccg aaatcttcct ggccgccagt agggcaacgt
     4501 tgttggttat ggccaacgac agggaaagtt atcatgccgt attcgacagc agatggcggc
     4561 aataaaggga ttgatatctc agctccacgg ggtacaccta tttacgccgc gggtgcagga
     4621 aaggtggtgt atgtgggcaa ccagctgcgt ggctacggta atctcatcat gattaaacac
     4681 agtgaagatt acattacggc ttacgcccat aatgacacga tgctggtaaa taatgggcaa
     4741 agcgtgaagg ctgggcaaaa aatcgccact atggggagca cggatgcggc atctgttcgc
     4801 ctgcatttcc agattcgtta ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg
     4861 cagggcagca agccaaaatg ctgatggcga attaatcagc agtcagcagc gcggcacttg
     4921 ctaaggagag cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt agttcaatgg
     4981 tagaacgaga gcttcccaag ctctatacga gggttcgatt cccttcgccc gctccatgac
     5041 ctttcttaaa gtctactaac ttactgaatc ctaagggaca tggcttaaag ccttccgata
     5101 gttttactca ctgtaaccaa cactgtgact aactttgttg acgtagtgag cagacctaag
     5161 gtctcagtac atgctcaact caagaaccta cctttatcag cgtaacggtg tcttttacat
     5221 ccgtcttagg atgaagacca ctggtcgcct gaccgcttcg cttccttctc acaaccgtta
     5281 taaactagcg tcagtatcac tacggaccaa ggatagacgt accgctatgg ctcactcacg
     5341 gcacatcaag tcagcactta aagcaatcca cgcagataac cctaacgcct cttatgagga
     5401 gctacgggaa cacctgaaga ccattgtcga gtgggaactt agtgtcagcc gcgatgacct
     5461 gaacgacccg gagtcctacc agctttacgt tgaccagtac gatgatatta agtccaacct
     5521 tcgggaagct gtagcgactg agcgtctgac tgtagaccag catcgctata tcaatgacgt
     5581 tatcggcgta cttaaaggct gtcaggatag actcaagggc gatagctctg gcctgttgtc
     5641 ttaccttgag cctgagacca gtagtctaag accatctgtc tccctatctg tatcatcgga
     5701 gcctgaagtc cccgaaccta aagctctgac cctcgcgtct ctcattgagc agtacgagca
     5761 ggagaacgct cagaactgga agccagcgac cctaagcgag aaccgagcgt cgcactccac
     5821 gctaatcgag atattagact atctggatat tcaggatgtc ggcaaggcga cccgtgcaga
     5881 tatgctcagg gtccgtgaag tcctccagct gttaccgaag aaccgcaagc aacgctttaa
     5941 gaccatgccg ctgtctgacc tgctgaaccg ggagtctaag aatgactgtc tagatgtcgt
     6001 taccatcaac aataaatatc ttatcaagat ggctgctgtg tttaagtggg cggtacgcaa
     6061 cgacctgata gctaagaacc tgacagaagg tcttgaactg aaagtccctc agcgtaaagc
     6121 ctccgacgct cgtggcgcgt tcagtcctga gcaggtaagg caactactgg tcgccgctaa
     6181 agcgtactct cagaagaccg caggtaagcc ataccattat tacgtcaccg ctctagctgc
     6241 ggttacaggt gcgagactta acgaggtggc ccagcttcag gtcaaagaca tcagggtcac
     6301 tgaggccggg actgtgtata tccatataaa cgaagacgat agcagcctac cgggtaagag
     6361 catcaagaac gctcatagcg accgctgtgt tccgctagta gatggcgctt atggttttgt
     6421 attggctgac tttatgtcgc ttgtggaaga ccgtagaaag actgagggtg ataatgctat
     6481 ggtattcgat ggcctcaagc tgatgaagaa tggctacggt gagcaggtta gtaaatggtt
     6541 taaccggacg ttgataccta aagtccttgc tgaccgtagc ggcttagcgt ttcattcgtt
     6601 taggcataca gttgcgaccc agttaaaaca acatggggtc gagttagcgt atgctcaggc
     6661 gattatgggt catagctcag ggtctataac ttacgaccgt tacgcgaaag aggtcgaggt
     6721 agatagattg gttaatgtga tggcgggtgt ttataaggag actggagtag atggcgatac
     6781 cgtttgaggc acctgagttt gaaaaagaag cctttaactg cccatattgt aacgcttttg
     6841 cacatcaaaa atggcagcag ttgtactatt actattcaag tactggaggc ctccctgttc
     6901 atacttctac ctgtgagcat tgtgaagaaa aaagtttttg gtttcagcgc cgcttactta
     6961 taccgtttgc ttccacagcc gaactaccaa acccggacat gcccgacaac tgtaaggctg
     7021 actacatgga agccagaagc atcgttggcc tgtcacctaa aggggccgct gcactccttc
     7081 gtctatgcct tcagaagcta atggtccact taggcgaacc ggggaataac attaacgctg
     7141 acatccggtc actcgttcag aaaggactac ctgtacgcat ccagcaggca gcagacatct
     7201 gccgtattgt cggcaatcag gcggtccatc ccggagagat tagccttgat gatgacccgc
     7261 agttggctca tggcctcttt aagctgctga acattattgt cgatgaccga atcacgagac
     7321 cgaaagaaat tgaggctatg ttccagagta tgcctgaagg tccacgccaa ggtattgaga
     7381 atcaggacag acaggcgagg gaacagcagc aggccgctaa cgaataaccc ctcactatag
     7441 gggaagggga cttaactatc actataggga gagaccctaa gtcaccagcc tgctggcatt
     7501 ctccgacaca catatacatt cattaagtct caggcttccg gtagcggacc gattggaaac
     7561 catccgcaga cgcctagtgt taaatcacct ggcctgagac cattctcttt aacgtaggtt
     7621 atcttccctt ttgacaggcg gggagcagaa gaccgcatcc gactttaagt tggctctgcg
     7681 ataatcgctg cgcggataac tgaagcacca agacatcatt gctgaggtct gccatccatt
     7741 tgtggacagg cggaaactca aagtggttga ccagtggagt ctgaggttgg actttgagtc
     7801 ctgaccagca ggctggagga gaccatgagt ttatcttgtt atggattgtg ttatcacaat
     7861 tccaataaga agaactttaa gtccagcctc cagtcctgtc agcccgaagt aaactatggt
     7921 cgctatgata tggtctccat
//
LOCUS       DAZXUK010000187         7785 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZXUK010000187 DAZXUK010000000
VERSION     DAZXUK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.187"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..773
                     /gene="nirC"
                     /locus_tag="ACH3L8_003104"
     CDS             <1..773
                     /gene="nirC"
                     /locus_tag="ACH3L8_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFT4086113.1"
                     /translation="AARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLLDPSV
                     RPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMWAILPQTWLGNLV
                     GSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALCNWLVCLAIWMAL
                     RTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYTLAGIGHNLLWVT
                     LGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            792..2165
                     /gene="cysG"
                     /locus_tag="ACH3L8_003105"
     CDS             792..2165
                     /gene="cysG"
                     /locus_tag="ACH3L8_003105"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFT4086114.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            2412..2579
                     /locus_tag="ACH3L8_003106"
     CDS             2412..2579
                     /locus_tag="ACH3L8_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFT4086115.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            2874..4211
                     /gene="frlA"
                     /locus_tag="ACH3L8_003107"
                     /pseudo
     CDS             2874..4211
                     /gene="frlA"
                     /locus_tag="ACH3L8_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            4232..5254
                     /gene="frlB"
                     /locus_tag="ACH3L8_003108"
     CDS             4232..5254
                     /gene="frlB"
                     /locus_tag="ACH3L8_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFT4086116.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            5304..6134
                     /gene="frlC"
                     /locus_tag="ACH3L8_003109"
     CDS             5304..6134
                     /gene="frlC"
                     /locus_tag="ACH3L8_003109"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFT4086117.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            6131..6916
                     /gene="frlD"
                     /locus_tag="ACH3L8_003110"
                     /pseudo
     CDS             6131..6916
                     /gene="frlD"
                     /locus_tag="ACH3L8_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            7016..7747
                     /gene="frlR"
                     /locus_tag="ACH3L8_003111"
                     /gene_synonym="yhfR"
     CDS             7016..7747
                     /gene="frlR"
                     /locus_tag="ACH3L8_003111"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFT4086118.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
BASE COUNT         1762 a         2033 c         2094 g         1896 t
ORIGIN      
        1 atgctgcgcg cattgcacgc ctgtcggcaa ataacccgct cggcttttgg gtcagctccg
       61 ccatggcggg cgcatatgtg ggtcttggga tcatcctgat tttcacgctc ggtaatttgc
      121 tcgacccgtc cgtacgtccg ctggtgatgg gcgcgacctt tggtatcgcc ttaacgctgg
      181 tgattatcgc cggttctgaa ctgttcaccg gacacaccat gttcctcacc tttggggtaa
      241 aagcgggcag catcagccac gggcaaatgt gggcaatcct gccgcaaacc tggctgggta
      301 acctggtcgg ttccgtcttc gttgccatgc tctatagctg gggcggcggt agcctgctgc
      361 cggtagatac cagcatcgtt cactccgtcg cgctggctaa aaccactgca ccggcaatgg
      421 tactgttctt caaaggtgca ttgtgtaact ggctggtttg cctggcaatc tggatggcgc
      481 tgcgcactga aggggcggcg aaatttatcg ctatctggtg gtgtctgctg gcatttatcg
      541 cgtccggcta cgagcactct atcgctaaca tgacgctgtt cgcgctctcc tggttcggca
      601 accacagcga agcctacacg ctggcgggta ttggtcataa cctgctgtgg gtgacgctgg
      661 gtaatacttt atcaggtgcc gtattcatgg gattgggtta ttggtatgct acgccgaaag
      721 cgaatcgtcc ggttgcggac aaatttaatc aaactgaaac agctgccggt taattactaa
      781 ggggttttta cgtggatcat ttgcctatat tttgccaatt acgcgatcgc gactgtctga
      841 ttgtcggcgg tggtgatgtc gcggaacgca aagcaaggtt gctgttagac gcaggcgctc
      901 gcttaacggt gaatgcatta gcgtttattc cacagttcac cgcatgggca gatgcaggca
      961 tgttaaccct cgtcgaaggg ccatttgatg aaagccttct cgacacctgc tggctggcga
     1021 ttgcagcgac ggatgatgac acgcttaacc agcgcgtcag cgaagccgct gaagctcgtc
     1081 gcatcttctg taacgtagtt gatgcgccga aagccgccag ctttattatg ccgtcgatta
     1141 ttgaccgctc accgctaatg gtagcggtct cctctggcgg cacctctccg gttctggcgc
     1201 gcctgttgcg cgaaaaactc gaatcactgc tgccgttgca tctgggccag gtggcgaaat
     1261 atgccgggca attacgcggg cgagtgaaac aacagttcgc cacgatgagt gagcgtcgcc
     1321 gtttctggga gaaattgttc gttaacgacc gcctggcgca gtcgctggca aacaacgatc
     1381 agaaagccat tactgaaacg accgaacagt taatcaacga accgctcgac catcgcggtg
     1441 aagtggtgct ggttggtgca ggtccgggcg atgccgggct gctgacgctg aaaggactgc
     1501 aacaaattca gcaggcagat gtggtggtct acgaccgtct ggtttctgac gatattatga
     1561 atctggtacg ccgcgatgct gatcgcgttt tcgtcggcaa acgcgcggga taccactgtg
     1621 taccgcagga agagattaac cagatcctgc tgcgggaagc gcaaaaaggc aaacgcgtgg
     1681 tgcggctgaa aggtggcgat ccgtttattt ttggccgtgg tggcgaagag ctggaaacac
     1741 tgtgcaacgc gggtattccg ttctcggtgg ttccgggtat taccgcagct tctggttgct
     1801 ctgcctattc gggtattcca ctcacgcatc gcgattatgc ccagagcgta cgcttaatta
     1861 ccggacactt aaaaaccggt ggcgagctgg actgggaaaa cctggcggca gaaaaacaga
     1921 cgctggtgtt ctatatgggg ttgaatcagg ccgcgactat tcagcaaaag ctgattgaac
     1981 acggtatgcc tggcgaaatg ccggtggcaa ttgtcgaaaa cggaacggca gtcacgcagc
     2041 gcgtgattga cggtacgctc acgcaactcg gtgaacttgc tcagcaaatg aacagtccat
     2101 cgctaattat tattggtcgg gttgttggcc tgcgcgataa actgaactgg ttctccaatc
     2161 attaatttaa cccgggccag agaattctgg ccttcttaat gactcttttt atatattcag
     2221 caaataaaac ataacccctt aatgaatatt atctgctaat gggttatatt caccgttaca
     2281 gcgatttaca aaattaagtc attcgctcac tgacgccaat taaatattcc tgtatcgttc
     2341 ttaacgagca gtctgcttat attcatataa tcaatgaata ttaattaata tataatacat
     2401 agggaatgta aatgaacaaa tttattaaag ttgcactggt aggtgcagta ctggctacgt
     2461 taactgcatg tactggtcat attgaaaacc gtgataagaa ctgctcttac gactacctgc
     2521 tgcacccggc aatttctatt tctaaaatca ttggcggttg cggtcctact gcacagtaag
     2581 tctctgacag aaatcggcta acaccggttt aattgcccga cgccctctgg attttccgag
     2641 ggcgtatttg tttctatgtc cttcttgccc ccgcccgtaa aaatattttc actttaaatt
     2701 caattttata actacatcca ttagccacag cgcgatcctg tgcgaaattt tgtgatcttc
     2761 ctccacatca cataacataa tatgttgtta tattcatcat gcattgtcat gttacctttt
     2821 aaatggctgc aaactctccc ctacaacgta ttggacaaga aaaaggtatc gctatgggaa
     2881 gccaggaact ccaacgcaag ctcggatttt gggccgttct tgcaatcgcc gtcgggacaa
     2941 ccgtcggctc cggtattttt gtatctgtgg gtgaagtggc aaaagcagcg ggcacgccgt
     3001 ggcttacggt gctcgcgttt gtcattggcg ggttaattgt gatcccgcaa atgtgcgtct
     3061 atgcggaact atccaccgct tatccggaaa atggcgcaga ttatgtttat ctgaaaaatg
     3121 ccggaagccg accgctggct ttcctctccg gctgggccag cttctgggcc aacgatgcgc
     3181 cgtcattgtc gattatggcg ctggcgattg tcagcaatct tggcttttta acgcctatcg
     3241 atccgttgct cggtaaattt atcgccgccg gattaattat cgcctttatg ttgctacacc
     3301 tgcgctccgt tgaaggcggc gcagcgtttc agacgctaat taccatcgcc aaaattatcc
     3361 cgttcactat cgtcattggc cttgggatct tctggtttaa agcggagaat tttgccgccc
     3421 ttaccaccac tgcgattggc gcaacgggca gctttatggc gctgctggcg gggatctctg
     3481 ccaccagttg gtcgtatacc ggcatggcct ctatctgtta tatgaccggc gaaattaaaa
     3541 accccggaaa aaccatgcca cgagcgctga ttggttcctg tctgctggtt ctggtgctct
     3601 acaccctgct ggcgctggtg atttccggcc tgatgccctt cgacaaactc gccaattctg
     3661 aaacgccgat ttccgacgcc ctgacctaga tccccgcact cggcagcacc gctgggctct
     3721 ttgttgccat cacggcgatg atcgtcattc ttggttcgct ttccagctgc gtgatgtacc
     3781 agccgcggct ggaatacgcg atggcgaaag acaacctgtt ctttaaatgc ttcggccatg
     3841 tgcatccgaa atacaacacg ccggatgtct ccatcatcct gcaaggggcg ctggggatct
     3901 tcttcatctt cgtttccgat ctcaccagcc tgctgggtta tttcaccctg gtgatgtgtt
     3961 tcaaaaatac cctcaccttc ggctccatca tctggtgtcg taaacgcgac gattacaaac
     4021 cgctgtggcg tactccggct ttcgggctga tgaccaccct cgccattgcg tcaagcctca
     4081 ttctggtcgc ctcaaccttt gtctgggcac cgattcccgg ccttatctgc gccgccatcg
     4141 ttattgctac tggtctgcct gcttacgcct tctgggcgaa gcgtagccgc cagctcaacg
     4201 ctttgtcgta atgttatctg aagaaaataa aatgttggat attgataaaa gcaccgtgga
     4261 ctttctggtc accgaaaata tggttcagga agtggaaaaa gttctcagcc atgacgttcc
     4321 gctggtgcac gccatcgtgg aagagatggt gaagcgcgac attgatcgta tttatttcgt
     4381 tgcctgcggc tcgccactca acgcggcgca aacggcgaaa catctggcgg atcgcttttc
     4441 cgatcttcag gtctacgcca tttccggctg ggagttctgc gataacaccc cgtatcgcct
     4501 cgacgatcgt tgcgcggtga ttggcgtttc tgactacggt aaaacggaag aggtaatcaa
     4561 agcgctggag ctgggccggg cctgcggagc actcactgcg gcgttcacca aacgcgcgga
     4621 cagcccgatt acctcggcgg cggaatttag cattgattat caggccgact gtatctggga
     4681 aattcacctg ctgctctgct acagcgtggt gctggagatg atcacccgcc tcgcgccgaa
     4741 cgcggaaatc ggcaagatca aaaacgatct caagcagttg ccgaatgcgc tcggtcatct
     4801 ggtacgcacc tgggaagaaa aaggccgcca gcttggtgaa ctggccagcc agtggccgat
     4861 gatttatacc gttgctgcgg gtccgctgcg tccgctgggt tacaaagaag gcattgtaac
     4921 gctgatggaa tttacctgga cgcacggctg cgtgattgag agcggagagt tccgccatgg
     4981 cccgctggag attgtcgaac cgggcgttcc gttcctgttc ctgctcggca atgatgaaag
     5041 tcgccacacc accgaacgcg ccattaactt tgttaaacag cgtactgaca acgtgatcgt
     5101 catcgattac gccgaaattt cgcaagggct gcacccgtgg ctggcaccgt tcctgatgtt
     5161 cgtgccaatg gagtggctct gctactacct gtctatttac aaagatcaca acccggatga
     5221 acgccgctat tacggtggtc tggtggaata ttaatccctc tcccggcccg gcaacgggcc
     5281 ggatttacgc aaggagttac ccgatgaaaa caggtatgtt tacctgcggc caccagcggc
     5341 tgcctattga acacgcattt cgtgatgcaa gcgagctggg ttacgacggc atcgaaattt
     5401 ggggcggtcg cccgcacgcg ttcgcgccgg acttaaaagc gggcggcatc aaacaaatca
     5461 aggcgctggc gcagacgtat cagatgccga ttatcggtta cacgccagaa accaacggct
     5521 atccgtataa catgatgctg ggcgatgaac atatgcgtcg tgaaagcctg gacatgatca
     5581 agctggcgat agatatggca aaagagatga acgcgggtta tacgctgatt tctgcggccc
     5641 acgcgggcta tctcacgccg cctaacgtta tctgggggcg gctggcagag aacttaagcg
     5701 aactgtgtga gtacgcggaa aacatcggca tggatctgat cctcgaaccg ttaacgccgt
     5761 atgaatcgaa cgtcgtgtgt aatgctaatg atgtgcttca tgcgctggcg ctggtgcctt
     5821 cgccgcgcct gttcagcatg gtcgacattt gcgcgccgta tgtccaggcg gaaccggtga
     5881 tgagttattt cgacaaactg ggcgataaat tacgtcatct gcatattgtc gacagcgacg
     5941 gggccagcga cacgcattac attcctggag aaggcaaaat gccgctgcgg gaactgatgc
     6001 gcgatattat tgatcggggc tatgagggtt actctacggt ggagctggtg acgatgtata
     6061 tgaacgagcc cagactctat gcccgccagg cgctggaacg ctttcgcgcg ctgctgccgg
     6121 aggatgagag atgaaaaccc tggcgacaat cggcgataac tgcgtcgata tctacccgca
     6181 actgaataaa gcgttttctg gcggtaatgc ggtcaatgtg gcggtgtact gcactcgcta
     6241 cggcatacag ccgggatgca ttacctgggt gggtgacgat gactacggca caaagctgaa
     6301 gcaggatctc gcccgcatgg gcgtcgatat cagccatgtc catacgaaac acggcgttac
     6361 cgcacaaact caggtggaac tgcacgacaa tgatcgcgtt tttggcgact acaccgaagg
     6421 cgtgatggcc gactttgccc tgagtgaaga ggattacgcc tggctggcgc agtatgacat
     6481 tgtgcacgcg gcaatctggg gacatgcgga agacgcattc ccacagctgc acgctgcggg
     6541 caaacttacc gctttcgact tctccgacaa gtgggacagc ccgctctggc agacactggt
     6601 gccgcatctc gattttgcct ttgcctccgc accgcaagaa gacgaagcgc tgcgtctgaa
     6661 gatgaaagcg attgttgccc gtggcgcagg aacagtgatt gtcacgctgg gtgaaaacgg
     6721 cagcattgcc taggatggcg cgcagttctg gcgtcaggct cctgaaccgg tgacggttat
     6781 cgacaccatg ggtgccggag attcgttcat tgccggattc ctttgcggct ggtctgcggg
     6841 gatgacatta ccgcaggcga tggcgcaggg aacggcgtgc gcggcgaaaa ccattcagta
     6901 ccacggtgcc tggtaggtat aacgttggcg tgagcatctt cacgccaacg tgctgttact
     6961 tgccggaaaa cgaccctata atccgagtaa ttcattcttt atttcagggt cgattatgtc
     7021 agctacggac cgctactctc atcaactcct ctacgctacc gtccgccagc ggctgctgga
     7081 tgatatcgcg cagggggttt accaggccgg gcaacagatc cctaccgaaa acgagctttg
     7141 tacacaatat aacgtcagcc gcattaccat tcgcaaagcc atcagcgact tagtggcaga
     7201 cggcgtactg atccgctggc agggaaaagg cacctttgta caaagccaga aagttgaaaa
     7261 cgccctgctt actgtcagtg gttttaccga ttttggcgtc tcacaaggca agtcgacgaa
     7321 agagaaagtg atcgaacagg aacgggtcag cgccgcgccg ttttgcgaaa agctgaacat
     7381 ccccggaaac agcgaagtgt tccatctctg ccgggtgatg tatctcgata aagagccgct
     7441 gtttattgat agttcatgga tcccgctgtc gcgttatcct gactttgatg agatttacgt
     7501 cgaaggaagc tccacctatc agttatttca ggagcgtttt gacacgcgag tggtcagcga
     7561 caaaaagacc atcgatatct ttgccgccac ccgcccgcag gcaaaatggc tgaaatgcga
     7621 actgggcgaa ccgttgtttc gcatcagcaa aatcgccttt gatcagaatg acaaaccggt
     7681 gcacgtctcc gaactcttct gccgcgccaa tcgcatcacc ttaacgattg ataataaaag
     7741 acattaaccg taggccggat aagatgcgcc agcatcgcat ccggc
//
LOCUS       DAZXUK010000188         7743 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZXUK010000188 DAZXUK010000000
VERSION     DAZXUK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.188"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="ACH3L8_003112"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="ACH3L8_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFT4086119.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="ACH3L8_003113"
     CDS             1102..2562
                     /locus_tag="ACH3L8_003113"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFT4086120.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="ACH3L8_003114"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="ACH3L8_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFT4086121.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="rspR"
                     /locus_tag="ACH3L8_003115"
                     /gene_synonym="ydfH"
     CDS             complement(2979..3665)
                     /gene="rspR"
                     /locus_tag="ACH3L8_003115"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFT4086122.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="ACH3L8_003116"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="ACH3L8_003116"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFT4086123.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="ACH3L8_003117"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="ACH3L8_003117"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFT4086124.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="ACH3L8_003118"
     CDS             complement(6727..7245)
                     /locus_tag="ACH3L8_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFT4086125.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7521..>7743
                     /gene="ydeI"
                     /locus_tag="ACH3L8_003119"
     CDS             7521..>7743
                     /gene="ydeI"
                     /locus_tag="ACH3L8_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFT4086126.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHK"
BASE COUNT         1940 a         1812 c         1956 g         2035 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 aacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca
     7561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
     7621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
     7681 tcgcaaaaaa catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca
     7741 aag
//
LOCUS       DAZXUK010000189         7463 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZXUK010000189 DAZXUK010000000
VERSION     DAZXUK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.189"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..74
                     /locus_tag="ACH3L8_003120"
     CDS             <1..74
                     /locus_tag="ACH3L8_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086127.1"
                     /translation="KAKGGRQKGEVVGVDDQCKEHKE"
     gene            292..534
                     /gene="yiiF"
                     /locus_tag="ACH3L8_003121"
     CDS             292..534
                     /gene="yiiF"
                     /locus_tag="ACH3L8_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFT4086128.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(864..1793)
                     /gene="fdhE"
                     /locus_tag="ACH3L8_003122"
     CDS             complement(864..1793)
                     /gene="fdhE"
                     /locus_tag="ACH3L8_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFT4086129.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1790..2425)
                     /gene="fdoI"
                     /locus_tag="ACH3L8_003123"
     CDS             complement(1790..2425)
                     /gene="fdoI"
                     /locus_tag="ACH3L8_003123"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFT4086130.1"
                     /translation="MKRRDTIVRYTALERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2422..3324)
                     /gene="fdoH"
                     /locus_tag="ACH3L8_003124"
     CDS             complement(2422..3324)
                     /gene="fdoH"
                     /locus_tag="ACH3L8_003124"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFT4086131.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3337..6387)
                     /gene="fdnG"
                     /locus_tag="ACH3L8_003125"
     CDS             complement(3337..6387)
                     /gene="fdnG"
                     /locus_tag="ACH3L8_003125"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5800..5802),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFT4086132.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6581..7414
                     /gene="fdhD"
                     /locus_tag="ACH3L8_003126"
     CDS             6581..7414
                     /gene="fdhD"
                     /locus_tag="ACH3L8_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFT4086133.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1701 a         1947 c         2003 g         1812 t
ORIGIN      
        1 gtaaagcaaa gggaggcagg caaaaggggg aggtagttgg tgttgatgat cagtgtaagg
       61 agcataagga atgaaaaaaa tttttcattc ttgaatataa aaaacagatg cccttattct
      121 ggtattaata caaggctgtt ttacttgaac ttataataac tgcaactgtt acatcgtatc
      181 tggaaaacgc ctcgcaaaaa taaaaaatga tgcgtaaatg agctttcttt aaagattcgg
      241 caagggtact tttctctcca tagattcaaa aatgatattg gcgagatatt tatgaactct
      301 ctggcaggta tagatatggg cagaatttta ctcgatttat cgaatgaggt gattaagcaa
      361 cttgatgatc tcgaggtgca gcgtaatctt cctcgtgcgg acctattaag ggaagcggta
      421 gatcaatacc tgataaatca atcgcaaaca gcaagaacca gtgttcctgg catctggcaa
      481 gggtgtgagg aagatggtgt cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa
      541 aaaatgatgt caatcagatt gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga
      601 acgccttatc cggcctacaa agtcatgcaa attcaatata ttgaagttta ttggtaggcc
      661 tgataagcgc agcgcatcag gcaatcatac gcttgtcatc agtgacatac ccataacctt
      721 atcgatgtgt tggagggaag aacgaggcaa acagacaatt gcggcatgat cttgaatggg
      781 taacgcggaa tacgagagat ggtgtcgtca caccctatga gctgtaatcc gggcgacctg
      841 cgccacccgg aaaacgtcag agattactct ccttcacccg gaaacaggaa cgggttaatg
      901 gaactacggg cgtagccttc ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca
      961 tctgccacgg cttcaacttt cggttctttt tcctgataaa gaatcttcag gtaagtgccg
     1021 cagtcatcgc agctttcggc tttaatcgcg gcctgttcgt catccagcga ccagtaatgc
     1081 agtttgccgc tctgctcgca gttgctgcat tttacgcgca ctacgtgcca ttcggtttca
     1141 cacaggttgc agtgcaggta acgcagaccc tgagtggtgc caatttgcac catgctggac
     1201 accggcatag agccacatac cgggcaatat tgacgttgtt cgccgtattc agcgcgggct
     1261 ttgccgggga tcagattggc catctgcgcc cagtagagcg acagcgcagc ccagataaac
     1321 ggcgctttat cgctgctgac ggacgagaaa tcagaggcaa acagcgcgct ggccatatct
     1381 tccagctcct gagtcgatgc cttctccaga ttctcaatca ctgccagcgc cgggccgctc
     1441 atttcaggtt tcagctcagc aattagcgcc atcagcagct tttgccagtg cttatcacgc
     1501 ggcagaacgt gaatatccag cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg
     1561 gtcagatcca tctccagcgg atggtcgtac agcaccactt tctgggcgtg ggcgataagc
     1621 gcagcaaagc gcaggtaatc acccagcgga ttattttctg ccagctcgcg cagacgctcg
     1681 gcgcggcggt tgtataaatt cttgagccga gggaacaata acggcggaat catatccgcc
     1741 gtacgtttct cgctcgaacc cagctcatct tgcgggatta tgcgaatact cattcagctt
     1801 tcttttccgt tgtcttgcgg acctcacggt accagcgcgg gtgatgtttc ttcgcccagg
     1861 cgctggtaac ccatccttcc accatggcgg taatcgtgcc tttcacccaa agggcggcgt
     1921 agatatgcac catgataacc acaattaacg ccactgcggc aaatgaatgc agcattaacg
     1981 cgaatcggat caccgggatt gagaaagcag gcgcaaaata aggacgccag ataatcacgc
     2041 cgctcaccag cagcagaacc aggaaaataa tcgccgccca gaaaacgcat ttctgaccga
     2101 agttataacg cccggtgtca cctacttcct cgttgacgac gatcttacga atattcttcg
     2161 cccaaaagat atcatcccga ttgattaggt tttgatgcca gtaacggaaa aacatgatga
     2221 tgaacgaggc aaacataacc acgccgacaa acgggtgcag aattcgcgcc agctgcggtg
     2281 tgcccatgat ttgcatcaac cagttgaagg acgggaacaa aaagcccagc ccgctcaccg
     2341 ccgccaggat gaagcagaag gcggtgatcc agtggttgat acgttccagc gcggtgtagc
     2401 gcacgatggt gtcacgtcgt ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt
     2461 ctcttcctca tccgcacggt tcggaccgac acccacgtag tggaagatac tggccgcaaa
     2521 ggtagccgca aagccaacag ccgcgagcgg tttccagatg cctttccaga atttcacggt
     2581 ttcgctgatt tccgggttct ccggcaagcc atgatacaga tttggcttgt cagcatggtg
     2641 cagcacgtac atgacgtgtg taccaccgac gcctgccgga tcgtacagac ccgcattgtc
     2701 gtaaccgcgg gttttcagct cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt
     2761 cgtgccgaag tgaatcgcgc ccgttgggca ggtcttcacg caggctggtt cttgcccaac
     2821 caccacgcgg tcaacgcaca gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg
     2881 cggaatgtcg aacggacagc ccgcaatgca ataaccgcag ccaatgcact gctcggactg
     2941 gaagtcgacg ataccgttgg catactgaat aattgcacct tccgccgggc acgctttcag
     3001 gcagcctgga tcggaacagt gcatacagcc gtctttgcgg atcagccatt ccagtttgtc
     3061 gttctgctcc acttccgaga agcgcattac cgtccacgat ttggcgctta aatcattggg
     3121 gttgtcgtac accccaatgt tattgccgac ggtatcgcga atgtcgttcc actctgaaca
     3181 cgccacctga caggctttac agccgataca ggtggtaacg tcgatgagtt tcgccacttc
     3241 ttcctggaag tcccgcgcct gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat
     3301 gatatcttga gattgataag ccatatgtcg tctccgttac accttttcca cattcacaag
     3361 gaaggactta aactccggcg tctgcgtgtt cgcatcacca acgaatggcg ttaaagtatt
     3421 ggcaataaag ccttttttag ctacgccttc atagccccag tgaatcggaa taccgatggt
     3481 atcgatatct ttgccgttcg ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc
     3541 tttgatatag ccacggttgg aggagacttt cacggtatcg ccctgggcaa tgccaagttt
     3601 attcgccagc gactccccga tttccacaaa ctgctctggc tgcaaaatcg cgttcaacag
     3661 cgcgtgtttg gtccagtagt ggaagtgctc ggtcagacga taggtggttc cgacatacgg
     3721 gaacttatcg gctttaccca atgcttcggc gtcgtcttta aagatacgtg cagctgggtt
     3781 cgagataacg tttggatgca gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg
     3841 ttccgggaac ggaccttccg ccatcttatc gagggcaaac agacgcccca tgccttcctg
     3901 ctgcatgata aacggcccga cgccgctacc cggaggcgct gcgctgtagt ccggaatatc
     3961 ccagccggtc cacttagtgc cgtcccattt cagcaactga cgcttcggat cccacgggtt
     4021 accctgcgga tctgcggagg cgcggttata cagaatgcgg cggttaagcg gccatgccca
     4081 tgcccagccc agcgtgttac cgaggccaga cggatcggcg ttatcacgac gcgccatctg
     4141 gttgccttcc ggcgtccagc taccggcgaa aatccagcag ccacaggagg ttgtaccgtc
     4201 atcgcgcaat tgggcgaacg agctaagttg ttggcctttc ttgacgataa ccgcaccggt
     4261 tgccggatcg gtaatatcgg ccagcgcctt accgttgctc tccatcgcta cttcttccga
     4321 cttcggctca tgcggaatgg cgtagttcca ggtcatgttc agcacctgat ccgggttcgc
     4381 gccaccctgt tcggcataca tcttgcgcaa gcgcaggaag ataccggaga ggatctcacc
     4441 gtcagtcagc gcaatccccg gggcgtccgc acctttccag tgccactgca accagcgacc
     4501 tgagttaacg attgaaccgt tctcttctgc gaagcaggtc gatggcagac ggaacacttc
     4561 ggtctggatc ttcgacgagt caacttcgtt cagctcaccg tggttctgcc agaagttaga
     4621 ggtttcagtg ttcagtgggt cgatggtgac gaggaacttc agtttcgaca gacaaccgat
     4681 cactttgttt ttgttcggga atgaggcaac agggttaaag ccctggcaga tatagccatt
     4741 gaccttgccc tctttcatca tctcgaagta ttgcaggacg tcgtagcctt tatcccactt
     4801 cggcaaccag tcaaagcccc agctattttc cgccgtcgct ttatcaccaa agaaggcctt
     4861 catcatagag acgaagaatt tcgggtagtt gccccagtag tttacctggc cttccagcag
     4921 cggttttggc gtgttggcgg taaggtaggt ttgcagatcg gtctgcttct cgcttggcag
     4981 cgtcatgtaa cctggcagac tctgcgacag tagccccagg tccgtcagcc cctgaatatt
     5041 ggagtgaccg cgcagggcgt taacgccgcc gccggccatc cccatattgc cgagcagcag
     5101 ctggatcatc gccatcgtac gaatgttttg cgcaccaacg gagtgttgcg tccagccgag
     5161 ggcatacagg aacgaggcag ttttatcgtg agcactggtt tctgcgatgt attcgcagac
     5221 tttcaggaac gcgtcttttg gcgtaccaca gatgttttca accacatctg gcgtgtagcg
     5281 ggaaacgtgc tgtttcagca agttccacac gcagcgcgga tgttgcagcg tggtatcgcg
     5341 tttggcgaag ccgttttcgt ccagttcata agtccaggag gatttatcgt acttgcgttt
     5401 ttccgcgtcg tagccggtga acaggccatc ttcaaagccg taatcctcac gcacgatcag
     5461 gctggcgttg gtataggctt cggtgtattc gtggttgaat ttttcattgt tcagcaggta
     5521 cagcaatacg cctgacagga aagcaatgtc agtaccggaa cgaatagggg catagtagtc
     5581 agctaccgcc gccgtacgcg taaagcgagg atcgatcaca atcagcttcg cgccgttgtg
     5641 aattttggct tccatcgccc agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc
     5701 catcaccacg acgaggttgg cgttcttgat gtcgacccag tggttggtca tcgcaccgcg
     5761 accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc
     5821 gaccgcgagc atacccagcg cgcgggagaa tttttgcgtt aaatagccgg tttcgttact
     5881 cgacgcggaa gcacacagca tcccggtgga gagccagcgg ttaacagtca cgccttcggc
     5941 gttttgcgca atgtagttag catcgcggtc ttctttcatc agtttggcga tgcgatcaaa
     6001 cgcctcttcc caactgattt gctgccattt atcagaacct ggcgcacggt attccggaaa
     6061 cttcagacgg ctttcggagt ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc
     6121 accgcggttg accgggtgat ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt
     6181 ttttgctccg tcaccgaggc tgtacatcaa cagcccacag cctacggaac aataggtgca
     6241 ggtattacgg gtttcgcggg tgcgcagcag tttatactgc cgggtttccg cgagtgctac
     6301 gctgggtgca aaacccagtg ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt
     6361 aaagaactgc cttctgctga cctgcatgga ttgctccttg tttcgacatt gtcacgtccc
     6421 atttacattc gcttgctgcg tgtgcaggga gtgggagtta tttttctttg cggaaggggc
     6481 cgcaaaggtc cagaattggc tcaatttccc tccatctagg aagggtttgt aacagaatac
     6541 cataatgttg gtgtgtatgt tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa
     6601 agaaatcgaa aatgtgacaa atatcacagg tgttcgtcaa attgagttat ggcggcgtga
     6661 tgatttacaa cacccacggt tagatgaggt cgcggaagaa gttcccgttg cgctggtcta
     6721 caacggcatt tcgcatgtgg tgatgatggc gtcgcccaaa gaccttgagt actttgcgct
     6781 cggtttttcg ctttccgagg ggataataga aagtccgcgc gatatcttcg gcatggatgt
     6841 cgttccttcc tgtaatggtc ttgaagtaca aattgagctt tccagccgcc gctttatggg
     6901 gttgaaggag cgccgccggg cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga
     6961 gcaacttaat gacatcggaa aaccggtgca gccgctaccg ttcacccaga cgtttgatct
     7021 caacaaactg gatgatgcat tacgtcatct caacgatttt cagccagtgg ggcgactgac
     7081 tggttgtact cacgccgctg cctggatgtt gccatctggc gaactggtcg gcgggcatga
     7141 agacgtgggt cgccatgtgg cgctggacaa actgttaggc cgccggtcac aagaagggga
     7201 aagctggcag caaggtgcgg tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa
     7261 gtcggcgatg tgcggcgtag agattttgtt tgcggtgtct gccgcgacca cgcttgctgt
     7321 agaagtggct gagcgctgta atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac
     7381 ggtttatacc catccgcagc gtttgagcaa ttaattaaaa taagtcctgc gatatatatt
     7441 aaattgcagg acttattcat ttc
//
LOCUS       DAZXUK010000190         7399 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZXUK010000190 DAZXUK010000000
VERSION     DAZXUK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.190"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            143..1540
                     /gene="ulaA"
                     /locus_tag="ACH3L8_003127"
     CDS             143..1540
                     /gene="ulaA"
                     /locus_tag="ACH3L8_003127"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFT4086134.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1556..1861
                     /gene="ulaB"
                     /locus_tag="ACH3L8_003128"
     CDS             1556..1861
                     /gene="ulaB"
                     /locus_tag="ACH3L8_003128"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFT4086135.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1871..2335
                     /gene="ulaC"
                     /locus_tag="ACH3L8_003129"
     CDS             1871..2335
                     /gene="ulaC"
                     /locus_tag="ACH3L8_003129"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFT4086136.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2349..2999
                     /gene="ulaD"
                     /locus_tag="ACH3L8_003130"
     CDS             2349..2999
                     /gene="ulaD"
                     /locus_tag="ACH3L8_003130"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFT4086137.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            3009..3863
                     /gene="ulaE"
                     /locus_tag="ACH3L8_003131"
     CDS             3009..3863
                     /gene="ulaE"
                     /locus_tag="ACH3L8_003131"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFT4086138.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            3863..4549
                     /gene="ulaF"
                     /locus_tag="ACH3L8_003132"
     CDS             3863..4549
                     /gene="ulaF"
                     /locus_tag="ACH3L8_003132"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFT4086139.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4678..4953)
                     /gene="yjfY"
                     /locus_tag="ACH3L8_003133"
     CDS             complement(4678..4953)
                     /gene="yjfY"
                     /locus_tag="ACH3L8_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFT4086140.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5280..5675
                     /gene="rpsF"
                     /locus_tag="ACH3L8_003134"
     CDS             5280..5675
                     /gene="rpsF"
                     /locus_tag="ACH3L8_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFT4086141.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5682..5996
                     /gene="priB"
                     /locus_tag="ACH3L8_003135"
     CDS             5682..5996
                     /gene="priB"
                     /locus_tag="ACH3L8_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFT4086142.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            6001..6228
                     /gene="rpsR"
                     /locus_tag="ACH3L8_003136"
     CDS             6001..6228
                     /gene="rpsR"
                     /locus_tag="ACH3L8_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFT4086143.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6270..6719
                     /gene="rplI"
                     /locus_tag="ACH3L8_003137"
     CDS             6270..6719
                     /gene="rplI"
                     /locus_tag="ACH3L8_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFT4086144.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6790..7353)
                     /locus_tag="ACH3L8_003138"
                     /pseudo
     CDS             complement(6790..7353)
                     /locus_tag="ACH3L8_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         1762 a         1807 c         2021 g         1809 t
ORIGIN      
        1 ctctatccat gtggaattat ttgcgggtcg cgtcacattt aatcataaat aatcttgttg
       61 tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga atacccggtt
      121 acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg tgttttttaa
      181 ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg gctacatcct
      241 actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa ttggtttcat
      301 gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg tggcgaaaat
      361 gtccgaagtc tacggcatta acggcgcaat ttccgatact tacgcgtcga tgatggcaac
      421 catcgaccgc atgggcgatg cctatagctg ggtaggttac gcggtgttgt tagcgctggc
      481 gctgaacatc tgttacgtgc tgctgcgtcg cattaccggt attcgtacca tcatgctgac
      541 tggtcatatc atgttccagc aggcggggct gattgccgtt acgttgttta ttttcggcta
      601 ctccatgtgg acaaccatta tctgcacggc gattctggtt tcgctctact ggggcattac
      661 ctccaacatg atgtacaagc cgactcagga agtgacggac ggctgcggtt tctccatcgg
      721 tcaccagcaa cagtttgcat cattaattgc ctataaagtt gcgccgttcc tcggcaaaaa
      781 agaggaaagc gttgaagacc tcaaactgcc aggctggctg aatattttcc acgacaacat
      841 cgtctccacg gcgattgtca tgaccatctt ctttggtgcc attctgctct ccttcggtat
      901 cgacaccgtg caggcgatgg caggcaaagt gcactggacg gtctatatcc tgcaaaccgg
      961 tttttccttc gcggtggcga tcttcatcat cactcagggt gtgcgcatgt ttgtggcgga
     1021 actctctgaa gcgtttaacg gtatctccca gcgcctgatc cctggtgcgg ttctggcgat
     1081 tgactgtgcg gctatttata gcttcgctcc gaacgccgtg gtctggggct ttatgtgggg
     1141 caccatcggt cagctgattg cggttggcat cctggtcgcc tgcggttcct cgatcctgat
     1201 tatcccaggc tttatcccga tgttcttctc caacgccacc atcggcgtgt tcgctaacca
     1261 cttcggcggc tggcgtgcgg cgctgaagat ttgtctggtg atggggatga tcgaaatctt
     1321 tggttgcgtc tgggtggtga aacttaccgg tatgagtgcc tggatgggga tggcggactg
     1381 gtcgattctg gcaccgccga tgatgcaggg cttcttctcc atcggtatcg cctttatggc
     1441 cgtcatcatt gtaattgcac tggcttatat gttcttcgct ggccgcgcac tgcgcgcaga
     1501 agaagatgca gaaaaacaat tggcagaaca gtctgcttaa taaggagttt tgattatgac
     1561 cgtacgtatt ctggctgtgt gtggcaacgg acaaggcagt tccatgatca tgaagatgaa
     1621 agtggaccag tttttaaccc aatcaaacat tgaccacacg gtaaatagct gcgcggttgg
     1681 cgaatacaaa agcgagttga gcggcgcgga tatcatcatc gcttctacgc acattgcggg
     1741 cgaaatcacc gtgaccggca acaaatacgt ggttggcgtg cgcaacatgc tctctcctgc
     1801 cgactttggc ccgaaactgc tggaagtgat caaagcgcat ttcccgcagg atgtgaagta
     1861 aggacgcgcc atgaaattac atgattcgct ggcggagaat aaatccatcc gcctgcaggc
     1921 tgaagctgag acatggcagg acgcagtaaa aatcggcgtt gacctgctgg tggcggcgga
     1981 tgttgtcgag ccgcgttact accaggcgat tctggatgcg gttgaacagc acggtccgta
     2041 ctttgtgctc gcaccaggcc tggcaatgcc gcacggtcgc ccggaagagg gcgttaagaa
     2101 aaccggtttt gcgctggtga cgctgaaaaa gccgctggag ttcaaccacg aagataacga
     2161 cccggtggat atcctcatca ccatggcggc ggtcgatgcc aacacgcacc aggaagtggg
     2221 catcatgcag atcgtcaacc tgtttgaaga tgaagaaaat ttcgaccgtt tacgtgcctg
     2281 ccgtaccgag caggaagtac tggatctcat tgaccgcacc aacgcggcag cttaagaagg
     2341 aattgaagat gtcattaccg atgttgcaag tcgcgctgga caaccagact atggatagcg
     2401 cctacgaaac cactcgcctg attgccgaag aagtcgacat tatcgaagtg ggtaccattc
     2461 tgtgcgtggg cgaaggcgtg cgtgcggttc gcgatctgaa agcgctctac ccgcacaaaa
     2521 tcgtgctggc agacgccaaa attgccgatg cgggcaaaat tctttctcgt atgtgcttcg
     2581 aagccaacgc tgactgggtg accgtgattt gttgtgcgga tatcaacacc gccaaaggtg
     2641 cgctggacgt agcaaaagag tttaacggcg acgtgcagat cgaactgacc ggttactgga
     2701 cctgggaaca ggcgcaacag tggcgcgacg caggtattca gcaggtggtt tatcaccgca
     2761 gccgtgacgc gcaggccgct ggcgtggcgt ggggtgaagc cgatatcact gcgatcaaac
     2821 gtctttccga tatgggcttc aaagtcaccg tcaccggtgg cctggcgctg gaagatctgc
     2881 cgctgttcaa aggcattcca attcatgtgt ttatcgccgg tcgcagcatc cgtgatgccg
     2941 cttctccggt tgaagccgcg cgtcagttca aacgttccat cgccgaactg tggggctaag
     3001 gagcggatat gttgtccaaa caaatcccgc ttggcatcta tgaaaaagcg ctccccgccg
     3061 gggagtgctg gctggaacgc ctgcgactgg caaaaacgtt gggcttcgat tttgtcgaga
     3121 tgtcggtaga tgaaactaac gagcgcctgt cgcgcctcga ctggagccgc gaacagcgtc
     3181 tggcgctggt gaatgctatt gttgaaaccg gcgtgcgtgt gccgtccatg tgcctttctg
     3241 cccatcgtcg tttcccgttg ggcagcgaag atgacgcggt gcgggcgcag gggctggaga
     3301 ttatgcgtaa agccatccag ttcgcccagg acgttggcat tcgcgtgatc cagctggcgg
     3361 gctatgacgt ttactatcag gaagccaata acgaaacgcg tcgtcgtttc cgtgacggcc
     3421 tgaaagagag cgttgagatg gcgagccgcg cgcaggtgac gctggcgatg gagatcatgg
     3481 attatccgtt gatgaactcc atcagcaagg cgctgggtta cgcgcactat ctcaacaacc
     3541 cgtggttcca gctctacccg gatatcggca acctgtcggc gtgggacaac gatgtgcaga
     3601 tggagttgca ggccggaatc gggcatatcg tcgcggtgca tgtgaaagac accaaaccgg
     3661 gcgtcttcaa aaacgtgcca tttggcgaag gtgtggtgga tttcgaacgt tgcttcgaaa
     3721 cgctcaaaca gagtggctat tgcgggccgt acctgattga gatgtggagc gaaacggcgg
     3781 aagacccggc ggcagaagtg gtgaaagcgt gtgattgggt gaaagcgcgc atggccaaag
     3841 caggcatggt ggaggcggca taatgcaaaa gctaaaacag caggtgtttg aagccaacat
     3901 ggatctgccg cgctatgggc tggtgacctt tacttggggc aacgtcagcg ctatcgaccg
     3961 cgaacgcggg ctggtggtga tcaagcccag cggcgttgcc tacgaaacca tgaaagcaga
     4021 cgatatggtg gtggtcgata tgagcggcaa ggtggtggaa ggggagtatc gcccatcttc
     4081 cgacactgcg acgcatctcg aactctaccg tcgttacccg tcgcttggcg gcattgtcca
     4141 tacccactcc actcatgcca ccgcatgggc gcaggcgggg ctggcgatcc cggcattagg
     4201 caccacgcac gctgactact tctttggtga tatcccgtgt acgcgtggat taagcgaaga
     4261 agaggtgcag ggtgagtatg aactgaacac tggcaaagtg attatcgaaa cgctgggcaa
     4321 cgccgagccg ctgcatacgc cgggaattgt ggtgtatcag cacggaccgt tcgcctgggg
     4381 gaaagatgcc cacgacgcgg tgcataacgc ggtagtgatg gaagaagtgg cgaaaatggc
     4441 gtggattgcg cgcagtatta atccacaact caatcatatc gacagctacc tgatgaataa
     4501 acacttcatg cgtaagcacg gtcctaacgc ttattacggg cagaagtaaa acacgcgctg
     4561 cggaaatttc cttcctcggg agataactgg tctaattccg cagccgtttt tcaaaaaaag
     4621 ccccctgcga agggggcaaa gcaaactatg gcaatgtttc gttggttata cctggtgcta
     4681 gcgataaata tccgcgctgg cgtgcatatt gccgttactc cccggttccc gcatcagaat
     4741 tacgtggtag tacgttgcgc cttgcgcatc tgtttcttca tttaatgcct gacgtgcttc
     4801 gctttcagtg gcgaaattat gattgatata aatcacgcct aagctttgca catcgtccat
     4861 atttctggcc tggtggttat taatttcaat ggctgcccat gtatttgcac ttagcaaaag
     4921 cacagccaga agggctaaaa ctcgactgaa catagatacc tcctcgacgg ctgactttgt
     4981 gtgctctcct ctgtgatgat cttctgattt aattttaatc aatgataaag aagttgatgg
     5041 tgaccatttc tgatgcagtt gttcaaaaaa acactgtgac ggaatgtgat gaacttcaaa
     5101 tcagcggttt agaggttatt tgcgaaaggg gagatttatt tctgctccgc ccttgagttt
     5161 agcggggtat gccagtacta taacgcgtca tttttcagcc gacctttaac acgttccttg
     5221 cctccccggg attcggctga cccagacagg aggctgaata atccgtaagg agcaattcga
     5281 tgcgtcatta cgaaatcgtt tttatggtcc atcctgacca gagcgaacag gttccgggca
     5341 tgatcgagcg ctacactgct gccatcactg gtgcagaagg caagatccac cgtctggaag
     5401 actggggccg ccgtcagctg gcttacccga tcaacaaact gcacaaagca cactacgttc
     5461 tgatgaacgt tgaagctccg caggaagtga tcgatgagct ggaaactacc ttccgcttca
     5521 acgatgccgt tatccgcagc atggttatgc gtaccaagca cgctgttacc gaagcatctc
     5581 cgatggttaa agcgaaagac gagcgccgtg agcgtcgcga tgatttcgca aacgaaaccg
     5641 ctgatgatgc tgaagctggg gattctgaag agtaatttct gatgaccaac cgtctggtgt
     5701 tgtccggcac cgtgtgcagg actccccttc gaaaggtcag tccatcagga attcctcact
     5761 gccagttcgt gcttgagcat cgttctgtgc aggaggaagc cggctttcac cggcaggcat
     5821 ggtgtcaaat gcccgttatt gttagcggac acgaaaacca ggccattact cacagtataa
     5881 cggtcggcag tcgcataacc gttcaggggt tcatttcatg ccacaaggca aagaacggac
     5941 tgagcaaaat ggttttgcat gccgagcaga ttgaattgat agattctgga gactagccat
     6001 atggcacgtt atttccgtcg tcgcaagttc tgccgtttca ccgcggaagg cgttcaagag
     6061 atcgactata aagatatcgc tacgctgaaa aactacatca ccgaaagcgg taagattgtc
     6121 ccaagccgta tcaccggtac ccgtgcaaaa taccagcgtc agctggctcg cgctatcaaa
     6181 cgcgctcgct acctgtccct gctgccgtac actgatcgcc atcagtaatc ggtcacggtc
     6241 cattaatacg actttgagag gataaggtaa tgcaagttat tctgcttgat aaagtagcaa
     6301 acctgggtag cctgggtgat caggtaaacg ttaaagcggg ctatgctcgt aacttcctgg
     6361 taccgcaggg taaagctgtt ccagctacca agaaaaacat tgaattcttc gaagcacgtc
     6421 gcgctgaact ggaagctaaa ctggctgaag ttctggcagc tgctaatgct cgcgctgaga
     6481 aaatcaatgc actggaaact gttaccatcg cgtctaaagc tggcgacgaa ggtaaactgt
     6541 tcggttccat cggtactcgc gacatcgctg acgctgtaac tgcagctggc gttgaagtgg
     6601 ctaagagcga agttcgtctg ccgaacggcg ttctgcgtac cactggcgaa cacgaagtga
     6661 gcttccaggt tcacagcgaa gtattcgcga aagtgatcgt aaacgtagta gctgaataat
     6721 tcgttattca acgagacgta aaaacgccga ccattggtcg gcgttttgct ttctattttt
     6781 cgtcaggcat taatttcgca agtagatcca ttcaccattc tcttgtgtcc ataagtcagg
     6841 atggtttgct ggcgttagat gcatacgatc actccttatg tctcggtcct gcggcactcg
     6901 ctcgaaacct aaacccgagt agattgcggc tgaatttgat aaggaacgta gaccaatttt
     6961 caaatcatgg ggtaaatcag aatacgcgtt tagcaatgca gcaatgatga atccgagggc
     7021 attagaaacg cctgtaacag tgtgaagttg gtcgatataa agatcgattg ttggtagttc
     7081 agatgagcag caattaggca tttgggcagg aagggttaca acgtatccta tcgtttggcc
     7141 ggtatagtct ggagtactat aaccaggtcc caaaacaacc tcgtgtattg tgtatttact
     7201 gttgggttcg aaggattcga catcttgcaa taaatatttc agttctgcgg ttaacctcgc
     7261 acccgcgtaa gctttattgt atttctgggc gtgaatatta tctaatgtag cttcatggtt
     7321 ccatccaaaa gaattatcac cattcattgt tactgaatcg ccacgggttt aacagacacc
     7381 tcagagtcat ttaagatgg
//
LOCUS       DAZXUK010000191         7390 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZXUK010000191 DAZXUK010000000
VERSION     DAZXUK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.191"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(154..1554)
                     /gene="asnS"
                     /locus_tag="ACH3L8_003139"
     CDS             complement(154..1554)
                     /gene="asnS"
                     /locus_tag="ACH3L8_003139"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFT4086145.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1723..2925)
                     /gene="pncB"
                     /locus_tag="ACH3L8_003140"
     CDS             complement(1723..2925)
                     /gene="pncB"
                     /locus_tag="ACH3L8_003140"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFT4086146.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3191..5806
                     /gene="pepN"
                     /locus_tag="ACH3L8_003141"
     CDS             3191..5806
                     /gene="pepN"
                     /locus_tag="ACH3L8_003141"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFT4086147.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(6013..6780)
                     /gene="ssuB"
                     /locus_tag="ACH3L8_003142"
     CDS             complement(6013..6780)
                     /gene="ssuB"
                     /locus_tag="ACH3L8_003142"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFT4086148.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(6777..>7390)
                     /gene="ssuC"
                     /locus_tag="ACH3L8_003143"
     CDS             complement(6777..>7390)
                     /gene="ssuC"
                     /locus_tag="ACH3L8_003143"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFT4086149.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         1860 a         1974 c         1884 g         1672 t
ORIGIN      
        1 ttattttttg tgcgtaaatg ttgcaatgta atattccgta acaggatgat cgttatggcg
       61 ggggtgcaaa tagattgctt gacttaacaa acagataact tgacagaaag gataaaaaag
      121 agccagcagg cgctggctct ttgaggaaga aaatcagaag ctggcgttgc gcggagtacg
      181 tgggaacgga atcacatcac gcacgttttg cacgccagtt acgtaagcaa tcaggcgttc
      241 aaaaccaaga ccgaaacctg aatgcggaac agtaccgtag cgacgcagat cgcgatacca
      301 ccagtaatct tctttattca ggcccatttc cagcatacgc tcgtccagaa cgtccagacg
      361 ttcttcacgc tgggagccac caatgatctc accgatgccc ggagccagaa cgtccatagc
      421 cgcaacggtt ttaccgtctt cgttgaggcg catatagaac gctttaatat ctttcggata
      481 gtttttaacc actaccggtg ctttaaagtg ttcttccgcc agataacgct catgctcaga
      541 agagagatcg acgccccagt aaaccgggtt ttcaaacttc ctgccgcagt tttcgagaat
      601 ggtcactgcg tcggtgtaat ccacctgcgc gaaatcggct tcaatgaagc gttccagacg
      661 tgaaacggca tctttatcta cgcgttcagc gaagaatttc atgtcgtctg cgcgttcttc
      721 gagaaccgct ttgaagacat atttcagcat ggcttcagcc agacccgcga tatcgttcag
      781 gttagcaaac gccacttccg gctccagcat ccagaattcc gccaggtgac ggctggtgtt
      841 ggagttttca gcacggaaag tcgggccgaa ggtataaatt ttggataatg cgcaagcgta
      901 ggtttcgccg ttcaactggc cagatacggt caggaaagac tctttaccaa agaagtcttt
      961 gtcgaaatcc actttgccct gatcgttacg cggcaggttt tccagatcca gcgtagaaac
     1021 gcggaacatt tcgcctgcac cttcggtatc agatgcggta atcagtggcg ttgaaaccca
     1081 gaagaatccc tgctcgttaa agaagcgatg cagcgcctgc gccagcgtat ggcgaacgcg
     1141 cgcgacggca ccaatcaggt ttgtgcgcgg acgcaggtga gcgacttcac gcagatactc
     1201 aatgctgtgg cgttttgccg ccatcgggta agtgtctgga tcttcaaccc aaccagcaac
     1261 ttcaaccttg ctggcctgaa tttcaaattg ttgcccctgg cccggcgatg ccacgacttt
     1321 acccgtcaca atgaccgagc agccggtggt cagacgcagg acgtcttcat tgtaattggg
     1381 cagagaatta ttgatgacag cctgtacagg atcaaagcag gaaccgtcat aaacggcgag
     1441 gaaggagatg ccagcttttg aatctcggcg ggtacgtacc catccgcgta cggtgacttc
     1501 gctgtcaacg gctacgcggc cctggagtac gtcggctaca ggcacaacgc tcataatatt
     1561 ctctctgtta atagtcggaa aaaataaaca cttgtccacc cgtaaatggg ggggatacct
     1621 atgttacctg gcatctgcaa tcagacaagc agaattcgca aatgcagcga aagatttcgg
     1681 aattaaaggt aaaaaaggga gccgattagc tccctgatga tattaactgg cttttttaat
     1741 atgcggaagg tcgaacgctt tgcgcagcgc ccgaacaaac gctttatcat ggcagatagt
     1801 tttgccaggg ctgtcagaaa gtttcgccac cggtttaccg ttacactcta ccaacttaat
     1861 cacaatattc aggggtttta cctgggggat atcgcaggtc aggcgagtcc caataccaaa
     1921 acttaattgc acgcgggaag agaagtggcg gtatagctca accgctttgc gtaaatccag
     1981 attgtcagag aaaaccagcg ttttactctg tggatcaatt cccagctttt cataatgtgc
     2041 aatggctttt tcaccccatt caaccgggtc gccagagtca tgacgcaggc cctgataccg
     2101 actagcgaac tcgacaccga aatcacgcag gaaagcatcc atagtgatgc agtcggttaa
     2161 tgcaatgcca agttggtcgg gatactcttc cagccaggca gcaagtgcag ctcgctggct
     2221 gttggctaga tccgggctga tttgctgatg tgcctggaac cattcgtgtg cctgtgttcc
     2281 catcggcgtg agggaaagcc gacgcgccag atcgtagttg ctggtgccca cgaaccagga
     2341 ttcctgttgc agacgcttaa cgatggtttc ttgtacttcg cgagaaaaac ggcgacgggt
     2401 gccaaaatcc atcagatgga agcgcgacat atcaagaccg gcggttaacg ccgagaagtc
     2461 gactaatttg ctttccagcg tgtcgagggc ttgcgcaacg tcggcctgcg gtgagcgata
     2521 gcgatgtacc atttcactga taaccgccag caaaggaact tcccagagga tgacttcacg
     2581 ccacggaccg cttaagcgaa tatccagctt gccattatcg ttggacacgg tgacttgttc
     2641 cgggttaaag cggaactcgc gtaaccagtt aagatagtcg gccttaaaga aaggcagggc
     2701 agagagccac tgatattcat catcctgcag gcgcaggtgc tgcatcgcct gaatctgttc
     2761 acgaatagca tcggcataaa tacccagcag atcgtcacct cggcaacgaa actccgccgc
     2821 gacatgcaca tcgtaatagt gatgaaacac ggcttgctgc atatgcaact tataagcatc
     2881 tgtatccagc aacgagtgca gaacaggaga agcgaattgt gtcataggtg cgcagtagcg
     2941 tcctcgtaca ggagcgttta gtacaataaa catcttcagg aactgctgga gtataccttg
     3001 tttcgcaatt tattgaaccc cgatcacacc atatgccacc tttctggtcg atggcattac
     3061 gcggtgtatg ttataaaaat gtagcaataa aggcgtttgt acctgaaaag atgaagattc
     3121 tgcatagcgc gatttacgca acaggaatag actgaacacc agactctata aaagatgcta
     3181 aaggttattt atgactcaac agccacaagc caaataccgt cacgattatc gtgcgccgga
     3241 ttaccagatt actgatattg acttgacctt tgacctcgac gcgcaaaaga cggtcgttac
     3301 cgcggtcagc caggctgtcc gtcatggtgc atcagatgct ccgcttcgtc tcaacggcga
     3361 agacctcaaa ctggtttctg ttcatattaa tgatgagccg tggaccgcct ggaaagaaga
     3421 agaagagggc gcactggtca tcagtaattt gccggagcgt tttacgctta agatcattaa
     3481 tgaaataagc ctggcggcga atacggcgct ggaagggctt tatcagtcag gcgatgcgct
     3541 ttgcacccag tgtgaagccg aaggtttccg ccatattacg tattatctcg accgcccgga
     3601 cgtgctggcg cgttttacca ccaaaattat tgccgataaa atcaaatatc ccttcctgct
     3661 ttccaatggt aaccgcgttg cgcaaggcga actcgaaaac ggacgccatt gggtacagtg
     3721 gcaggacccg ttcccgaaac cgtgctacct gtttgcgctg gtggcaggcg actttgatgt
     3781 actgcgcgat acctttacca cgcgttctgg tcgcgaagta gcactggagc tgtacgtcga
     3841 tcgcggcaac cttgatcgcg cgccgtgggc gatgacctcg ctgaaaaact ccatgaaatg
     3901 ggatgaagaa cgctttggcc tggagtatga cctcgacatc tatatgatcg tcgcggtgga
     3961 tttcttcaat atgggcgcaa tggagaataa gggtctgaat atctttaact ccaaatatgt
     4021 gctggcccgc accgacaccg ccaccgacaa agattacctc gatattgaac gcgttatcgg
     4081 ccatgaatat ttccataact ggaccggtaa ccgagtgacc tgccgcgact ggttccagct
     4141 cagcctgaaa gaaggtttaa ccgtcttccg cgatcaggaa ttcagctctg accttggttc
     4201 ccgcgcagtt aaccgcatca ataatgtgcg caccatgcgc ggattgcagt ttgcggaaga
     4261 cgccagcccg atggcgcacc caatccgccc ggacatggtc attgagatga acaacttcta
     4321 caccctgacc gtttacgaga agggcgcgga agtgatccgt atgatccaca ccctgctggg
     4381 cgaagaaaac ttccagaaag ggatgcaact ctatttcgag cgtcatgatg gtagcgcagc
     4441 gacctgtgac gactttgtgc aggcgatgga agatgcgtcg aatgttgatc tctcccattt
     4501 ccgccgttgg tacagccagt ccggtacgcc ggttgtgacc gtcaaagacg actacaatcc
     4561 ggaaactgag cagtacaccc tgaccatcag ccagcgcacg ccagccacgc cggatcaggc
     4621 agaaaaacag ccgctgcata ttccgtttgc catcgaattg tatgacaacg aaggtaaagt
     4681 gatcccgttg cagaaaggcg gtcatccggt gaattccgtg ctgaacgtca cccaggcgga
     4741 acagaccttt gtttttgata atgtctactt ccagccggtg cctgcgctgc tgtgcgaatt
     4801 ctctgcgcca gtgaaactgg aatataagtg gagcgatcag caactgacct tcctgatgcg
     4861 tcatgcgcgt aatggtttct cccgttggga tgcggcgcaa agtttgctgg caacctacat
     4921 caagctgaac gtcgcccgtc atcagcaagg gcagccgctg tctctgccgg tacatgtggc
     4981 tgacgctttc cgcgcggtgc tgctcgatga gaagattgat ccggcgctgg cggcagaaat
     5041 cctgacgctg ccttctgtca atgaaatggc tgaactgttc gatatcatag acccgattgc
     5101 tattgccgaa gtacgcgaag cactcactcg tactctggcg actgaactgg cggatgagct
     5161 gctggctatt tacaacgcga attaccagag cgagtaccgt gttgagcatg aagatattgc
     5221 aaaacgcact ctgcgtaatg cctgcctgcg cttccttgct tttggtgaaa cgcatctggc
     5281 tgacgtgctg gtgagcaagc aataccatga agcaaacaat atgaccgatg cgctggcagc
     5341 gctttctgcg gcggttgctg cacagctgcc ttgccgtgac gcgctgatgc aggagtacga
     5401 cgataagtgg catcaggacg gtctggtgat ggataaatgg tttatcctgc aagccaccag
     5461 cccggcggcg aatgtgctgg agacggtgcg tggtctgttg cagcatcgct catttaccat
     5521 gagcaacccg aaccgtattc gttcgttgat tggtgcgttt gcgggcagca acccggcagc
     5581 gttccatgcc gaagatggca gcggttacca gttcctggtg gaaatgctta ccgacctcaa
     5641 cagccgtaac ccgcaggtag cttcacgtct gattgaaccg ctgattcgcc tgaaacgtta
     5701 tgatgccaaa cgtcaggaga aaatgcgcgc ggcgctggaa cagttgaaag ggctggaaaa
     5761 tctctctggc gatctttacg agaagataac taaagcactg gcttgatact tttgagccaa
     5821 aatgcctgat gcgacgcttg acgcgtctta tcaggcctac aactccgttg aaattgctgg
     5881 ttttgtaggc tggataaggc gttcacgccg catccggcag ccgtgcccaa ctgcctgatg
     5941 cgatgcttga cgtgtcttat caggcctaca agtccgttga aaatgctggt tttgtgcgcc
     6001 acatccggca tattaaccat gacgccgaat cagttgttca gaatgcccac gccgcattac
     6061 ccgttgcaat acttccgctt ccagctccgc taatcgcacc gaccctaatc gacgcggacg
     6121 ggggatatcc actgtcaaat ccagactaat ttttccctct tcaattaaca acacccggtc
     6181 agccattgct accgcttcgc tcacatcatg cgtcaccaac agtacggtaa agccgtgctg
     6241 ctgccaaagt gacacaatca aatcctgcat ctcgagtcgc gttaaggcgt ccagtgcccc
     6301 gagcggttca tcaagcaaca ataaacccgg tcgatgaatc aacgctcttg ccagcgccac
     6361 acgctgtttc tgcccgccag aaagtgcggc aggccattcc ccggcgcgat tctccagtcc
     6421 tgccgcagcc aacgcccgac gcgcggcatc gcgccactga cctttaaggc ctaacccaac
     6481 gttatcaatc accgatttcc acggcagcag acgcgcatcc tgaaacatca ttcgcgtatc
     6541 atcctgaatt tcagccagcg gtgtggtgcc cgctaacaca tcgcctgcgg ttggcgtttc
     6601 cagaccggcc agcaggtgca gcagggtact tttaccacca ccgctgcggc ccaccaccgc
     6661 cacaaactga cctgccggaa tatgtaaatc cagttggttc aggacgatat tttccgcgta
     6721 atgtttgctt actgcattga gcaacaatgg tgtgccctgg ttcagacgag cagtattcat
     6781 atcgtggcct ccttcaaatg ataagccggg ttccagcgca accagaggcg ctctaacagc
     6841 tgcgcgctga cgtcagcaag tttgccgagc agggcgtaaa gaataatagc gactaccacc
     6901 acgtctgttt gcaaaaactc tcgcgcattc atcgccagat aaccaatgcc ggaattggca
     6961 gaaatggttt cggcaacaat cagcgtcagc cacatcaggc ccaacgcaaa acgcacgccg
     7021 accataattg agggcagggc atcgggcagg atcacatgga taaacagcgg tatgccggat
     7081 aatccatagc tacgcgccat ctccaccagc ccgcgatcga tattacggat gccatgccag
     7141 gtgttgatat aaatggggaa cagcgtaccg agcgccacca gaaagatttt cgcggattca
     7201 tcaatgccaa accacaaaat caccagcggg atcagcgcca gatgcggcac gttgcgcaac
     7261 atctgaattg aggtatccag cagcctttct ccccagcgcg acagtccgct aatcagtccc
     7321 agaatcaggc ccagcgatcc gccaattgaa aagccaatca gcgcccgcca ggaactgatc
     7381 gccagatgct
//
LOCUS       DAZXUK010000192         7380 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZXUK010000192 DAZXUK010000000
VERSION     DAZXUK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.192"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..690
                     /locus_tag="ACH3L8_003144"
     CDS             <1..690
                     /locus_tag="ACH3L8_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFT4086150.1"
                     /translation="CILDDSNFAQIKMADGTLNACSAMHVQFYNAAMNRANIKNTFLD
                     YSNFYMAYMAEVNLYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNLNKAILQNIN
                     LIDSKLFCTWLTNTFLEMVICTGSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYR
                     VNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAM
                     KITADIMQHAD"
     gene            complement(844..2493)
                     /locus_tag="ACH3L8_003145"
     CDS             complement(844..2493)
                     /locus_tag="ACH3L8_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFT4086151.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2644..3993)
                     /gene="ghxP"
                     /locus_tag="ACH3L8_003146"
                     /gene_synonym="yjcD"
     CDS             complement(2644..3993)
                     /gene="ghxP"
                     /locus_tag="ACH3L8_003146"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFT4086152.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4699..5163)
                     /gene="soxR"
                     /locus_tag="ACH3L8_003147"
     CDS             complement(4699..5163)
                     /gene="soxR"
                     /locus_tag="ACH3L8_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFT4086153.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5249..5572
                     /gene="soxS"
                     /locus_tag="ACH3L8_003148"
     CDS             5249..5572
                     /gene="soxS"
                     /locus_tag="ACH3L8_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFT4086154.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5575..7152)
                     /gene="pdeC"
                     /locus_tag="ACH3L8_003149"
                     /pseudo
     CDS             complement(5575..7152)
                     /gene="pdeC"
                     /locus_tag="ACH3L8_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
BASE COUNT         2045 a         1930 c         1694 g         1711 t
ORIGIN      
        1 tgtattctcg atgactcaaa tttcgctcag ataaaaatgg ccgacggaac tctaaatgca
       61 tgctccgcta tgcatgttca attctacaat gcagcaatga atagagccaa tattaaaaat
      121 accttccttg actattcaaa tttttatatg gcgtacatgg ctgaggtaaa tctttataaa
      181 gtaatagcgc catatgttaa tttatttaaa gccgacctta gtttctctaa actcgattta
      241 attaactttg aacatgctga tctgtctcgc gtcaatctga acaaagcaat cctccagaat
      301 ataaacttaa ttgatagcaa actcttttgt acgtggctga caaatacatt cctcgaaatg
      361 gttatatgta ccggctctaa tatggctaat gttaatttta ataatgccaa tttaagcaac
      421 tgccatttca actgttctat tttaacaaaa gcctgtatgt ttaatacccg tctctatcgg
      481 gttaatttcg atgaggctag cgtccaggga atgggcattt ccattctccg tggggaggaa
      541 aatatcccca ttgatagtga taccctggta acactacaga aattctttga agaagattgt
      601 acctctcata ctggcatgtc acaaactgag gataatatta atgcagtcgc tatgaagatt
      661 actgcagata ttatgcaaca cgcagattga ggttaagtcg aagaacagcc aatgaattga
      721 ctttacaaga gagataactt atttgtttta gaatcatgaa tgttcgcccg agctaacctg
      781 ttaaaggtgc aaaagattgc acctttttca gattacctcg ggcctggtta cgtcgctttc
      841 ggcttactga ttttcctcaa ttagcaacgc ttcaagcaaa tcgagatcgt gcagtaattt
      901 ttgcagcgtt tcgttgctga tctcccgcgt ggcgcgcagg tggtacagtt cagcacgttc
      961 agaacgcaat gccgccagac ggaagcgacg ctcaaggttc tcttcctgca cggaactttc
     1021 aacgtcgtta cgtccatcgg cgcgacgacg caggttacca atgacgcgag aactgacctc
     1081 cgtaagcaac tggttatcga tattctcttc ggtatccgcc gccagacgct cctccatttt
     1141 ctggatcgcc acaatcgcca cttctgccgt tgccgctcgc gcaatacgtt cttctttcaa
     1201 ttgttgcgaa tggtccgcga cttcaatgtg ttgtagcaga ataggcaaca tcaccacgcc
     1261 gacaaacagc gagaagagaa tgacgccagc cgccaggaac accagctcat agcgcgccgg
     1321 gaagacgtta ccatccggca agagcagcgg gattgagagc acaccggcca gagtgatcgc
     1381 cccacgcacc ccagcgaaag acgcgatcag gatttctcgt gtggtccacg aaccaaactc
     1441 catcggcttc ttcttcagga agcggttgct gaactttttc atcgtccaca gccagccgaa
     1501 acggaccagc atcagcgccg catatatcag aataatatcg gtaaacagca tccagatttc
     1561 gacgttaggg tcgatttctg ccgccatcag cgacgtctcc agaatacccg gcagttgcag
     1621 acctaacagc aggaacacca tgccgttaaa gacaaattcc agcatcgccc aggtgctgtt
     1681 tgcgcgcagg cgcattgcca gtggcgcacg gcgcatcaca ccggagcggg tgatggtcat
     1741 cccggcggca accgcagcga ggatgcccga aacgccaata tgttcggcaa tcagataaga
     1801 agcgaatggc agcaagaaca gcagaacgat ctgcgtcgcg ggttcatcac cgccccagcg
     1861 gctgaggaat cgcagcgagc gaccatacag ccagctcacc acaaaaccgg caagaatacc
     1921 gccaatggct actttcataa attcgaccgt cgcaccgccg acagtgaaga tcatcgtccc
     1981 catcgccact gccacggcaa acttcaacga cactaggccg gaagcgtcat tcatcaacgc
     2041 ttcgccctgc aaaatgccca tgattttttt cgggatgcgc ccttcgccaa caatcccgga
     2101 gagcgccaca gcatcggtcg gagaaagtac cgccgccagc gcaaaggcgg ggatcagcgg
     2161 aatgcccggc accacccagt aaataaggaa gccaatgccg accacggtga ccaccaccag
     2221 cgccagcgcg aggccgaaaa tctctcgacc atgttcgaga aattcacggg tcggcgtttt
     2281 ccagccatca gcgaacagca acggcgggat aaacaagact aaaaagagtt caggatcaaa
     2341 ctccacatgc aaaccaaacg tcggccacgc cagtagcgca ccgatggcga tttgcataag
     2401 cggaagcggg atctgaaagg gcatgacacg agtgaccacc ccggacagcg agaccacgag
     2461 ggtcattatc agtatggtga agaagatttc catgcgttcc ctgtttgcga tgttttttat
     2521 gatctgagaa agacgttatg cctgaattct cattttttca gaatatcgca aaagctcaca
     2581 caatgtgcgg agaaaataaa aacgggcagc cattgccacc cgttttcaga atccgatgaa
     2641 agattagata gcccagccac ccgcatagaa ggtcaccagc gccacggcga taacgaccgt
     2701 accgatgttc aacttgcgcc attcaccgga aaccagacga ccaatcacca gagtcgcgaa
     2761 gccgatcatg atgcctgtta cgatgttaca ggtcagcacg atgaataccg ccgtaaccag
     2821 acccgccatc gcatcaacaa aatcagcaaa gtcgattttc gccacgttgc tcagcatcag
     2881 caggccaacg tacatcagcg ccggagccgt tgcatacccc ggaacgaggt aagaaagcgg
     2941 agagaggaac agaatcagca ggaacagcac gccaacggtg atagccgtca acccggtttt
     3001 accgcccgcc gccgtacccg ccgcagactc gatgtatacc gctgccggag ctgcacccac
     3061 caggccagag aaaacgctgc tcatggagtc agtggtcagt gctttcccgc cgtcgatgat
     3121 ctgcccatct ttatccagca ggttcgcctg gccggcgaca gcgcggatag ttccggtggc
     3181 atcaaatact gccgtcatca ccagcgccag aacgcttggc aggactacag gattcagcgc
     3241 gcccataatg tccaggctgc caatcagtga attgccgttt tcatcgctca atgaaggcat
     3301 ggcgaaaacg ccggagaaat ggacgttagg atcgaagatc aaaccgacaa ttgagatacc
     3361 gataatggtc agcagaatgc caccagggac tttcagtttt tccaagccga tgatcaccgc
     3421 cagacctgcc agtgacataa tcaccgggaa ggtcgcgaaa tcacccagcg caacgggcag
     3481 accatcaagc gggtttttaa tcaccagacc gacaccatta gcggcaatga gcagcaggaa
     3541 cagaccgata ccgatccccg tgccgtgcgc cacaccgtgc ggcaagttgc gcaaaatcca
     3601 gctacggata cccgtggcag aaattaccgt aaacagcaca cccatcagga acacggcacc
     3661 cagcgcgaca ggtacgctaa tatgttgccc cagcaccagg ctgaatgcgg taaacgccgt
     3721 cagggagatg gcgcaaccaa tcgccaacgg cagattagcc cacagaccca tcacgataga
     3781 accgagtccg gcaaccagac aggttgcaac gaaaactgcc gcaggcggga agcctgcttt
     3841 acccaacata cctggaacga cgatgaccga gtagaccatc gccagaaacg ttgttaaccc
     3901 ggcaaccact tcctgacgga cagtgcttcc acgttgtgaa attttaaacc aggcgtcgag
     3961 tgaaccgccg gtacgcgctg atggcgtaga cataagaaaa catcccctga gagtttaatt
     4021 ttcgtcaaga tgcgtggcgg acaatcccct gccagcgaaa cgttatcttc ctgctccagg
     4081 tttgcggcaa tttggtgcgt cgcaaaaaag caaacgttta gctccgcgca tacaaaacgg
     4141 gtggtaaaaa aaagcaaacg attatccggc gatcatagcg gctttttcaa ccgaagtttg
     4201 ctgctttttt ctcttttatt gtcttgacga tgaaaaacca ggcaattgat gcgcaaacgt
     4261 tatcgtcgat gtgcaagatt gcagcgttat cgagccgtat accgttctgg attgcgataa
     4321 aaaatcgtct gagatgacaa aaatattgcg gctaaaaagt atacttttac actggcgaag
     4381 aaacgtcggt gacatacaat taaagcatca acaccaaccg gaacctccac cacgtgctcg
     4441 aatgaggtgt gttgacgtcg ggggaaaccc tcctgtgttc cagcgggata gagagaaaga
     4501 caaagaccgg aaaacaaact aaagcgccct tgtggcgctt tagttttgtg tttgcggata
     4561 attccaaaag agctcgatca gtcccctcgc cccttcgggg agagggttag ggtgagggga
     4621 acaggcagca ccagtgcaaa tatttaccct caccccggcc ccatagttaa cgatgagtca
     4681 gcttgtcctt gtggcgcttt agttttgttc atcttccagc aagcgtgcgc cggtaccttc
     4741 ttctcctaag cggtcgcccg ggttacgcaa cgggcaatca ctgcgcgaaa ggcagccaca
     4801 accaatacat ccgtccagtt cgtcacgcag cgccactaag gtatgaatgc gccgatccaa
     4861 ctcttctcgc cattgggacg aaagctgttt ccactctttc gcacttaacg tatgcccttc
     4921 tggcaacacg ccaaacgctt caccaatggt cgccagcgga atgccaatac gctgagcgat
     4981 tttgataatt gcgacatatc gcaacacatc acgtttatat cgccgctgat tgccgctgtt
     5041 acggatacta gtaatcaacc ctttgctttc atagaaatgc agcgccgata ccgccacacc
     5101 gctgcgtttc gccacttcgc caggggttag cagcgcttta atgcggggta atttcttttc
     5161 cataaatcgc tttacctcaa gttaacttga ggaattatac tccccaacag atgaattaac
     5221 gaactgaaca ctgaaaagag gcagatttat gtcccatcag aaaattattc aggatcttat
     5281 cgcatggatt gacgtgcata ttgaccagcc gcttaacatt gatgtcgtcg caaaaaaatc
     5341 aggctattca aagtggtact tgcaacggat gttccgcacg gtgacgcatc agacgcttgg
     5401 cgattacatt cgccaacgcc gcctgttact ggccgccgtt gagttgcgca ccaccgagcg
     5461 tccgattttt gatatcgcaa tggacctggg ttatgtctcg cagcagacct tctcccgcgt
     5521 tttccgtcgg cagtttgatc gcactcccag cgattatcgc caccgcctgt agttttattg
     5581 cccgcgcgtt aactcccgcg cgggtaattg ctccatccat tgcataaaca cctgcggcgg
     5641 catcgccttc gcaaaaaacc atccctggca atagcgcacg ccgcgcttgc gcagccagtt
     5701 aacctgctcc tcagtttcga cgccttcagc gatcgttttt aaccccaggc tgtgcgccag
     5761 ctcgatgatg tgttccgcaa tcaaatgact ggttttgtgg gtggtcagcg tttcaacaaa
     5821 cgatttatcg attttcaaaa tatcgacatt caatgattta aggttatgca agttagagta
     5881 gccaatacca aaatcatcaa ttgccacttc gtaaccagcc tggcggaaag ccagaattaa
     5941 tcggcgtcat tttttcgaca tcaagaaatg catgctcagt cacttcaaat ttaatttgct
     6001 gcggacgcac cgcgtattgc tctgttttct gattgattcg cgctatcaac cgtgacgtat
     6061 gaaaatcgga ggccgacagg ttaatagaaa catagcgatc ggcatgtgtt gccaggtacg
     6121 cgcccagatc gcggaagaca ttatcaataa cataatcagt tatctgttct atcatcccct
     6181 ccttttctgc cagcggaata aactctgccg gattcattat ttgcccctgc tcaccaggcc
     6241 aacgtaacaa cgcttcagcg ccgatacatt tttctgtttt gatatcgatt attggctggt
     6301 aataaagaca aagttgatgt ttttcgaggg cgcgttgcaa tttacgtttg ggagataaat
     6361 agttttgtcg aatacgtagc cagagtaata gcagaacaag actccccaaa ataccggcgg
     6421 gcaacgcaaa aataagatga ttataaaaat gggttataag acgttgatat gaagtcgcaa
     6481 caatggctgc aattgggcgt tttgtcgaat aaattgtcgc atataaatag ccatttcttt
     6541 gtacggttaa atccttcaaa tgaatcagcg gagaaaacgt tgctgccgag gcctctttgc
     6601 ttaacgagaa aaaggttttc atcaccgtat catacacacc ccattgcaat gtcgggtcat
     6661 cagacatcac ttcactccag aagagagggt tgataaccgc cacataattt ccccgctgca
     6721 tataggtcat tttatagcca gagaaaaaag gcgtatcgcg gtaataatag atagaaacgt
     6781 taggttcacg cttataatcg gccggtgcaa tcgtatagcc gtttacaggc gctatcagcg
     6841 atgagcataa aaaatggtta tcacgaattc attaatataa agatagccac gaataatatt
     6901 caacattcgc ttttgatggg ctggagtgca aacttgcccc tgatagcgtt cagctgcatc
     6961 gctcactaaa tctgcctggc gaatgaccag ctcggattta tccagtgcca gttgagcaaa
     7021 ggttcggagc tgattattca cttctgattt tgcccaaagg aaggcaatcc atagcgaaag
     7081 aatgatggga aagagaacta aaaagataat gcccggcaac gccagtaatt ggtgtcgtgc
     7141 acgatgactc attgctatca atttcctgcc tgtttatacc cggccattgc aaggtgaaac
     7201 cctggctacc gacgtcttta gataaatctt acggtataaa tagcactttt gcctgatttg
     7261 aaattgatac ctttttaatc gtcacctgga tgaaaataat tcattgatat tcaaaattaa
     7321 ttttctacag gaatacagag gcggcgggaa gatatgccct taatgccact gaaaaaccac
//
LOCUS       DAZXUK010000193         7355 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZXUK010000193 DAZXUK010000000
VERSION     DAZXUK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.193"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..846
                     /locus_tag="ACH3L8_003150"
     CDS             <1..846
                     /locus_tag="ACH3L8_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFT4086155.1"
                     /translation="NTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDLLIALES
                     RLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLELTDNQG
                     ENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLDFLPLYD
                     VDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTREATPGKE
                     IEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKT
                     APQAK"
     gene            complement(1049..1402)
                     /locus_tag="ACH3L8_003151"
     CDS             complement(1049..1402)
                     /locus_tag="ACH3L8_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFT4086156.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1540..3186)
                     /gene="groL"
                     /locus_tag="ACH3L8_003152"
     CDS             complement(1540..3186)
                     /gene="groL"
                     /locus_tag="ACH3L8_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFT4086157.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3230..3523)
                     /locus_tag="ACH3L8_003153"
     CDS             complement(3230..3523)
                     /locus_tag="ACH3L8_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFT4086158.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3799..5055
                     /gene="yjeH"
                     /locus_tag="ACH3L8_003154"
     CDS             3799..5055
                     /gene="yjeH"
                     /locus_tag="ACH3L8_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFT4086159.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5071..5547)
                     /locus_tag="ACH3L8_003155"
     CDS             complement(5071..5547)
                     /locus_tag="ACH3L8_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFT4086160.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5884..7320
                     /gene="aspA"
                     /locus_tag="ACH3L8_003156"
     CDS             5884..7320
                     /gene="aspA"
                     /locus_tag="ACH3L8_003156"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFT4086161.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
BASE COUNT         1830 a         1882 c         1770 g         1873 t
ORIGIN      
        1 aataccggtg ttattcgcaa gagtaataat ttcattgcac tggcgctgaa aattaaagaa
       61 cctcgcaata aagaatcgtt gttctttatg tctgtcatgg aactgcgcga cctgctgatt
      121 gcgctggaaa gtcgtctgca tcaaaagcat aaactggatg ctgctgcacg ccttcagtat
      181 gagcaagctc gcgataaagt cattaagaaa atggcagaaa acatcccaga aattctggtt
      241 gatgagctta aaaatgccga tattaatcgt cgggttaaca cgctggaact taccgataat
      301 caaggcgaaa atcttacctt cgtattaacg ctacatgacg gtagcaaatg tgagttagtc
      361 gtcaatgagt tgcagattga aatgctggcg cgagcaatca ttcatgcaat taataatgcg
      421 gaaatgcgtg aactggcatt acgtattacc tcactgttag atttcctgcc gttatacgac
      481 gttgactgcc aggacaatgg caatctggaa tatgacacct attcccaacc ggaatggaaa
      541 cataacttgt tcgaccatta tctggcggtg ctttaccgct ttaaagatga aagtggcaaa
      601 gaacaattta gcggcgcagt ggtgaaaacg cgtgaagcaa cgccgggtaa agaaatagag
      661 gcgatcactc gccgaatgct tgatttcagc ccacgactga aaaaactcgc gggtgtgcca
      721 tgtcaggtct atgttcgcac cgtagcggcg aataacgccc aaccgctaac ccaggatcaa
      781 tgcttacgcg cgctgcatca cttacgtgta caatccacca gcaaaactgc accacaggcg
      841 aaataatctc atctttgcta atgcgaggct ggagccatcc agcctgaaat gactgataac
      901 gtgatcgttg gtcatgccgg atgcggcgta aacgccttat ccggcctaca aattctcaag
      961 cattcaatca gttgcaatgc ctgctgtagg cctgataagc gaagcgcatc aggcaataat
     1021 acgtttgtca cccagcctct ggatcacatc agttcgggca cttataaacc tgaccgataa
     1081 tctggctatc cgtcgggaca aaactggaca acattccctg cgacgggctg ctgatgccat
     1141 aaatcacgtt accgcccatt gcagccgcct ggttgcgcag atcgtttgct gcgccgcgca
     1201 tagaaccgcc ctcttctccg tgttgcccgg aaagccagtt gctttgctta cctgtcgcag
     1261 taccaatcag ctggcactct gcgcctggct gctcgtccac aatgcgtaca ctctgaccgg
     1321 cagcactcaa ttcgttgctg gagctacaac ccgccatcag tagcgcggca ccgacaatcc
     1381 ctgctaagta ttttacgtgc atgttattcc ccataatcaa tgagctggac gccactcgcg
     1441 tcatccgtgt ctgaatctta tactagaaag atgaccaaaa gaaaaacccc cagacatttc
     1501 tgcccggggg tttgtttatt tctgcgaagt gcagggcaat tacatcatgc cgcccatgcc
     1561 acccatgccg cccataccgc cagcagcgcc taagtcagct gcatcgtttt tcggcaggtc
     1621 ggtaaccatg cattcggtgg tgatcatcag gccagccaca gaagctgcgt actgcagagc
     1681 agaacgggtt actttggttg ggtccaggat acccatgtcg atcatgttgc cgtattcttc
     1741 ggttgctgcg ttgtaaccgt agttgccgtc gccgccttta acggtgttag caacaacaga
     1801 cggttcttcg ccgcagttca ggacgatctg acgcagcgga gcttccattg cacgcagtgc
     1861 aactttgata cccacgttct ggtcttcgtt ctgaccacgc aggtcagcca gtttagacgc
     1921 tacgcggatc agcgcaacac caccaccagc aaccacgcct tcttctaccg cagcacgggt
     1981 cgcgtgcagg gcatcttcaa cgcgtgcttt tttctctttc atttcaactt cggtagcagc
     2041 acctactttg ataactgcaa cgccgcctgc cagtttcgct acgcgctcct gcagtttttc
     2101 acggtcgtag tcagaagttg cttcttcaat ctgctgacgg atctgagcaa cacggccctg
     2161 gattgcagct tcttcaccca cgccatcgat gatagtggtg gtgtctttgt tgatcacaac
     2221 acgtttagcc tgacccaggt cttccagggt tgctttttcc agctccatac cgatctcttc
     2281 agagattacg gtaccgccag tcagggttgc gatatcctgc agcatagctt tacgacgatc
     2341 gccgaagccc ggtgctttaa ccgcagcaac tttcacgatg ccacgcatgg tgttaacaac
     2401 cagagttgct agcgcttcgc cttcaacatc ttcagcgatg atcagcagcg gtttgcctgc
     2461 tttggcaaca gcttccagaa ccggcagcat ttcgcggatg ttggagattt tcttgtcagc
     2521 cagcaggatg aacgggcttt ccagttctac tgcgccagtt tccggcttgt tgatgaagta
     2581 aggagacagg tagccacggt cgaactgcat accttcaacc acgtccagtt cgtcctgcag
     2641 accggtaccg tcttcaacgg tgataacgcc ttctttaccg actttgtcca tcgcttcagc
     2701 gatcagttta cctacggttt cgtcggagtt agcggagatg gtaccaacct gagcaatcgc
     2761 tttagaatca gagcacggta cggacagcgc tttcagttct tcaactgcag cggtaaccgc
     2821 tttgtcgata ccacgtttca ggtccatcgg gttcatgccc gcagcaacag ctttcagacc
     2881 ttcagtgatg atagcctgag ccagtacggt tgcagtggtg gtaccgtcgc ctgcagcgtc
     2941 gttcgctttg gaggcaactt ctttcaccat ctgcgcaccc atattttcga acttgtcttc
     3001 cagttcgatt tcacgagcaa cggaaacacc atctttggtg atggtcggtg caccgaaaga
     3061 tttatccaga actacgttac ggccttttgg accgagggta actttcactg catctgccag
     3121 tacgtttacg ccgcgcagca ttttcacacg agcgtcgtta ccgaatttta cgtctttagc
     3181 tgccattatc tttattcctt aaattcgtat gttcagtgtc gtgcgcggat tacgcttcaa
     3241 caattgccag aatgtcgctt tcggacatga tcaacacttc ttcattgtcg atcttctcgg
     3301 atttcacacc gtagccatcg ttgaaaataa cgatgtcgcc aactttcacg tccagcggct
     3361 tcacttcgcc attttcaagg atacggccat tgccgacagc cagcacttcg ccgcgggtgg
     3421 atttagccgc tgcagagccg gtcagaacga tgccgccagc agatttagtt tcaacttctt
     3481 tacgcttgac gatcacgcga tcatgcaatg gacgaatatt cattgataac tctcctttga
     3541 gaaagtccgt atctgttatg ggtgacgccg gagcttacgt ggtttcccgg ctggtgacca
     3601 gagaaatggg gatgaggctt cgccccttca agggggaaaa agaaaaaaaa ttctgatcag
     3661 cacaaaatcg ggtgaaaacc ctgattcacc tcacatttca tcgcaatttc ttcatcgccg
     3721 tcataagcga atctgattgt gctaccatcg aaaatctacg cagttgccca aaatttgggc
     3781 gcaatcggat atcaacccat gagtggactc aaacaagaac tggggctggc ccagggcatt
     3841 ggcctgctat cgacgtcatt attaggcact ggcgtgtttg ccgttcctgc gttagctgca
     3901 ctggtagcag gcaataacag cctgtgggcg tggcccgttt tgattatctt agtgttcccg
     3961 attgcgattg tgtttgcgat tctgggtcgc cactatccca gcgcaggcgg cgtcgcgcac
     4021 ttcgtcggta tggcgtttgg ttcgcggctt gagcgagtca ccggctggct gtttttatcg
     4081 gtcattcccg tgggtttgcc tgccgcacta caaattgccg ccggattcgg ccaagcaatg
     4141 tttggctggc atagctggca actgttgttg gcagaactcg gtacgctggc gttggtgtgg
     4201 tatatcggta ctctcggtgc cagttccagt gctaatctac aaaccgttat tgccggactt
     4261 atcgtcgcac tgattgtcgc tatctggtgg gcgggcgata tcaaacctgc gagtatcccc
     4321 ttccccgcgc caggaaatat cgaacttacc gggttatttg ctgcgttatc agtgatgttc
     4381 tggtgttttg tcggtctgga agcatttgcc catcttgcct cggaatttaa aaatccagag
     4441 cgtgattttc ctcgtgcttt gatgattggt ctgctgctgg caggattagt ctactggggc
     4501 tgtacggtag tcgtcttaca cttcgacgcc tatggtgaac aaatggcggc ggcagcatcg
     4561 cttccaaaaa ttgtagtaca gttgttcggt gtaggagcgt tatggattgc ctgcgtaatt
     4621 ggctatctgg cctgctttgc cagtcttaac atttatatac agagcttcgc ccgcctggtc
     4681 tggtcgcagg cgcaacataa tcctgaccac tacctggcac gcctctcttc tcgccatatc
     4741 ccgaataatg ccctcaatgc ggtgcttggc tgctgcgtgg tgagcacgtt ggtaattcat
     4801 gccttagaga tcaatctgga cgctcttatt atttatgcca atggcatctt tattatgatt
     4861 tatctgttat gcatgctggc aggctgtaaa ttattgcaag gtcgttatcg actactggcg
     4921 gtggttggcg ggctgttatg cgttctgtta ctggcaatgg tcggctggaa aagtctctat
     4981 gcgctgatca tgctggtggg gttatggctg tttctgccaa aacgaaaaac gccggaaaat
     5041 ggcataacca cataatccgg cgtttcgaca ttaatcctgg cgatcgtctt tatgatcaag
     5101 gcggtcgcgg tcatcatcct ttcgctgata ctcaccatca aaagtattac cgccaccggt
     5161 cccggcgcta aaaccgccgc ctggcatgcg agaaaagcgc aaatgtggca tcaacttcac
     5221 tgtcagatgc ttttgcaccg gcggcaataa aagcagaaga ccgaggaagt cggtgaaaaa
     5281 gcccggtaat aaaagcagca aaccggcaat aatcagcgaa acacttttaa tcatctccgc
     5341 cgcagggttt tcacccgccg ccattttttg ctgcatcagc acgaaattct taaagccctg
     5401 gttacgtacc agtgacatac cgataactga cgtgaatata accagcacga gggtcagcaa
     5461 tacccccaat acatgggcaa cctgaataaa gattgaaatc tcaatataga cataaaggaa
     5521 aatggcaata aaaggtaacc agcgcaaagg tttctcctgt aatagcagcc ggttaaaccc
     5581 ggctacctga atgggttgcg aatcgccttc agcttatatt gtggtcatta gcaaaatttc
     5641 aagatgtttg cgcaactatt tttggtagta atcccaaagc ggtgatctat ttcacaaatt
     5701 aataattaag gggtaaaaac cgacacttaa agtgatccag attacggtag aaatcctcaa
     5761 gcagcatatg atctcgggta ttcggtcgat gcaggggata atcgtcggtc gaaaaacatt
     5821 cgaaaccaca tatattctgt gtgtttaaag caaatcattg gcagcttgaa aaagaaggtt
     5881 cacatgtcaa acaacattcg tatcgaagaa gatctgttgg gtaccaggga agttccagct
     5941 gatgcctact atggtgttca cactctgaga gcgattgaaa acttctatat cagcaacaac
     6001 aaaatcagtg atattcctga atttgttcgc ggtatggtaa tggttaaaaa agccgcagct
     6061 atggcaaaca aagagctgca aaccattcct aaaagtgtag cgaatgccat cattgccgca
     6121 tgtgatgaag tcctgaacaa cggaaaatgc atggatcagt tcccggtaga cgtctaccag
     6181 ggcggcgcag gtacttccgt aaacatgaac accaacgaag tgctggccaa tatcggtctg
     6241 gaactgatgg gtcaccagaa aggtgaatat cagtacctga acccgaacga ccatgttaac
     6301 aaatgtcagt ccactaacga cgcctacccg accggtttcc gtatcgcagt ttactcttcc
     6361 ctgattaagc tggtagatgc gattaaccaa ctgcgtgaag gctttgaacg taaagctgtc
     6421 gaattccagg acatcctgaa aatgggtcgt acccagctgc aggacgcagt accgatgacc
     6481 ctcggtcagg aattccgcgc tttcagcatc ctgctgaaag aagaagtgaa aaacatccaa
     6541 cgtaccgctg aactgctgct ggaagttaac cttggcgcaa cagcaatcgg tactggtctg
     6601 aacacgccga aagagtactc tccgctggca gtgaaaaaac tggctgaagt cactggcttc
     6661 ccatgcgtac cggctgaaga cctgatcgaa gcgacctctg actgcggcgc ttatgttatg
     6721 gttcacggcg cgctgaaacg cctggctgtg aagatgtcca aaatctgtaa cgacctgcgc
     6781 ttgctctctt ctggtccacg tgccggcctg aacgagatca acctgccgga actgcaggcg
     6841 ggctcttcca tcatgccagc taaagtaaac ccggttgttc cggaagtggt taaccaggta
     6901 tgcttcaaag tcatcggtaa cgacaccact gttaccatgg cagcagaagc aggtcagctg
     6961 cagttgaacg ttatggaacc ggtcattggc caggccatgt tcgaatccgt tcacattctg
     7021 accaacgctt gctacaacct gctggaaaaa tgcattaacg gcatcactgc taacaaagaa
     7081 gtgtgcgaag gttacgttta caactctatc ggtatcgtta cttacctgaa cccgttcatc
     7141 ggtcaccaca acggtgacat cgtgggtaaa atctgtgccg aaaccggtaa gagtgtacgt
     7201 gaagtcgttc tggaacgcgg tctgttgact gaagcggaac ttgacgatat tttctccgta
     7261 cagaatctga tgcacccggc ttacaaagca aaacgctata ctgatgaaag cgaacagtaa
     7321 tcgtacaggg tagtacaaat aaaaaaggca cgtca
//
LOCUS       DAZXUK010000194         7346 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZXUK010000194 DAZXUK010000000
VERSION     DAZXUK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7346
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.194"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            65..385
                     /locus_tag="ACH3L8_003157"
     CDS             65..385
                     /locus_tag="ACH3L8_003157"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086162.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(451..683)
                     /locus_tag="ACH3L8_003158"
                     /pseudo
     CDS             complement(451..683)
                     /locus_tag="ACH3L8_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(793..1563)
                     /gene="yafV"
                     /locus_tag="ACH3L8_003159"
     CDS             complement(793..1563)
                     /gene="yafV"
                     /locus_tag="ACH3L8_003159"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFT4086163.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            1744..2190
                     /gene="ivy"
                     /locus_tag="ACH3L8_003160"
     CDS             1744..2190
                     /gene="ivy"
                     /locus_tag="ACH3L8_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFT4086164.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2233..4677)
                     /gene="fadE"
                     /locus_tag="ACH3L8_003161"
     CDS             complement(2233..4677)
                     /gene="fadE"
                     /locus_tag="ACH3L8_003161"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFT4086165.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4917..5495
                     /gene="lpcA"
                     /locus_tag="ACH3L8_003162"
     CDS             4917..5495
                     /gene="lpcA"
                     /locus_tag="ACH3L8_003162"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFT4086166.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5701..6468
                     /locus_tag="ACH3L8_003163"
     CDS             5701..6468
                     /locus_tag="ACH3L8_003163"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFT4086167.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6439..7179)
                     /gene="dpaA"
                     /locus_tag="ACH3L8_003164"
     CDS             complement(6439..7179)
                     /gene="dpaA"
                     /locus_tag="ACH3L8_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFT4086168.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
BASE COUNT         1802 a         1931 c         1851 g         1762 t
ORIGIN      
        1 tagttcatgg gcaaattctt ggaaataaag ttagccgatg taattagtta gtggagagga
       61 agttgtggac gaaattagaa taagaattgc tggtaatacg gctgcgcctt gtttccatgt
      121 gctaaaaaat aagggatata aagtaacaat ggaaactcat gcattatctg acgattgttt
      181 agattgtgta tatgattggt atgcggaaaa aaatggacaa ttttttagtg caacgagtcc
      241 agaagagtta cttggattag ttgcaatgtg ggaagttcgt ggtaatgact ggagagctaa
      301 tttgaatgag ataaatactt atcaaaaagc catagaagaa tccccgtttt ttgataaaaa
      361 cggcaatgaa atagagtctg actaaatttt attgatatag caacgataat aaccggaaat
      421 ttcttccaga tctttccggc tttttattat ttaactttcg ttcagcacac taaacatccc
      481 gtctcgcatc cccttaactg gctgtttaac ctgataaagt tccctgcgca gttcctgtac
      541 ttcgttgtta tcccaagcag gatcaaacat ccctccgaga tcttcacggt aacgcccgtg
      601 ccgatctcaa atcccgcttt tctcagccat ttggcgctga ttttgatcac cggcgtgtcg
      661 ctgcggttgg gcagataacc cacggtacag cgacgctgtg ttttggaaat ggtgacttct
      721 ggcgtaatat tgtgtttaag ttaggggctt tcgtaatcta gcccagcccc cggccctaaa
      781 cacaaccccc actcaccaca acctaaactc atccgcatcc cgccatgccg gaaacttttc
      841 tctatattcc cgcaatgcca ccatcgacag ctccgcatca atacgcgttg ccttatgcgc
      901 gtcggcggta gcgataatct cgccttgcgg attaatcacc cggctgtcac cgcgataatg
      961 gcagccgttg ccatcgctgc cgacgcgatt gcatcccgcc acatacgcct gattctcaat
     1021 cgcgcgggcc gtcagcaatg cctgccagtg cagagagcgc ggagctggcc agttggcgac
     1081 gtacagggcg aggtcataat cgttgagatt gcgcgaccac actggaaaac gtaagtcgta
     1141 gcacaccagc ggcaaaatac gccagccgcg ccattccaca atcactcgcg cattgcccgc
     1201 tttataatgc agatgctcat ctgccatgcg gaacagatga cgcttatcat aaaaatgtac
     1261 cgtgccgccc ggctcaacca gcaaaaagcg gttaaccgaa ccagactccg tttgtaatgc
     1321 aacactgcct gcaatcagcg cattgcactg ctgcgcctta gctgtcatcc agttcactac
     1381 gtcatcttgt gcaagcgacg aagctgccgc ttccatggca aagccgctgg taaacatctc
     1441 cggtagaacg atcacatcgc gcccggtaat accttccagt tgacgatcaa aatggcgcag
     1501 gttggcagga ccatccaccc acaccaacgg ttgctgcaaa agcgtaatct tcaaactagg
     1561 cacggtgtac aactccttta tgcgaagggt tttataactt taacacctta tcaggcagtt
     1621 gccttagcgc agaataaatt gataacaaat gctgatattg gaaatatctg atttgcaaat
     1681 tatcgtgtta tcgccaggct ttaggaggtt aataacatgg gcaggataag ctcgggagga
     1741 atgatgttta aggcaataac gacagtcgcc gcactggtca tcgccaccag tgcaatggcg
     1801 caggatgatt taaccattag cagccttgca aagggcgaaa ccaccaaagc tgcgtttaat
     1861 cagatggtgc aagggcataa gctacctgca tgggtgatga aaggcggtac ttatactccc
     1921 gcacaaaccg taacgttggg agatgagacg tatcaggtga tgagcgcgtg caaaccgcat
     1981 gactgtggct cgcaacgtat cgctgtgatg tggtccgaga aatctaatca gatgacgggg
     2041 ctgttctcgg ctattgatga gaaaacgtcg caagagaaac tcacctggct gaatgtgaac
     2101 gatgcgcttt cgattgatgg taaaacggtg ctgttcgcgg cgttgaccgg cagcctggaa
     2161 aaccatccgg atggctttaa ttttaaataa ttagcggata aagaaacgga gcctttcggc
     2221 tccgttattc aattacgcgg cttcaacttt ccgcactttc tccggcaact ttaccggctt
     2281 cgtcgccagc tcttccggat caaagtcatc aacgttaata ctgcgcagac ggctttcttc
     2341 cgctttcacc agaatagcgg cttcatcttt atcaatcagc cccttcgtca gcgcgttgtg
     2401 cgccagttca tccagacggg taaatggcag atttttaccc agctctttac agatccgctg
     2461 atgaatcggg tcggcggcaa tcacatccac caacgcctct tccagtaaac caaccgggtt
     2521 atgctcgctt ggcgtcaggt actgaccgcg accaatacgt gaacgagtgg cgttcggcac
     2581 ttgtaaaatc ttcgccattt tatgatccag cttgtcagac ggtgccagat aatgacgtcc
     2641 ggtcgggaag atcaccacat tcagcagccc ggcaaccacg cggttcggga agttttgcag
     2701 taaattgtcc attgcctgtt ctgcctgata cagcgcatcc tgtacgcccc agtgcaccag
     2761 cggcagatcg gcttcattac ggccttcgtc gtcataacgc ttcagaacgg cagaggcgag
     2821 gtaaagctgg cttaaaatat cccccagacg tgccgagata cgctcacgac gtttcaggct
     2881 gccgcccagc agtgccatcg agacatcaga aagcagggcg aggttggcgc tcaggcggtt
     2941 caggtgctga tagtagcgtt tagtggcatc gccggttggc gtgctgctgg ttaaaccgcg
     3001 cgtcaggccc agccagaagc tgcgaacttt gttgctaccg acgtgaccga tatgtttgaa
     3061 caacagttta tcgaacgcgt tgacgtcatt gttcttcgcc gcttccatct cttccagcac
     3121 gtacggatgg caacgaatcg ctccttgtcc gaagatcatc atgctgcggg tcagaatgtt
     3181 agccccttca acggtgatgg caatcggtgc gccctggtaa gcacgcgcca ggaagttgct
     3241 ttgcccgagc ataatgcctt taccgccggt aatatccatc gcatcaataa tcgactgctg
     3301 cccgcggtgg gtacagtgat acttaacgat agccgacagc acggcaggtt tttcgccgag
     3361 cataatgccg taggtaatca gcgatgccgc agcatccatt acataagcgt tcccggcaat
     3421 acgcgccagc ggctcttcaa tcccttccat cttaccgata gagattttga actgacggcg
     3481 aatgtgagaa tacgcgcccg ttgccagcgc taccgatttc acgccgccgg ttgagttgga
     3541 aggcagggtg atgccgcggc ctaccgagag gcactccacc agcatccgcc agccttgccc
     3601 ggccattttc ggcccgccga tgatgtaatc gatcggcacg aagacatctt taccgcgcgt
     3661 cggtccgttc tggaacggta cgttcagcgg gaagtggcga cgaccaattt ccacgcccgg
     3721 cgtggtggtt gggatcagcg cacaggtaat gcctaaatct tctgcaccgc cgagtaattt
     3781 ttccgggtcg gagagtttaa acgccagccc aagcacggtc gcaatcggtg ccagcgtaat
     3841 gtagcgtttg ttccaggtca gacgcatccc cagcacctgc tggccctgcc attcgcccat
     3901 gcagacaatc ccggtgtccg gaatcgcgcc cgcatcggaa cccgcttccg ggctggtcag
     3961 tgcaaagcag gggatctcct gaccacgcgc cagacgcggc agatagtgat ctttctgctc
     4021 gtcagtgccg taatgttgca acagttcgcc cgggcctaat gagtttggca cgccgacggt
     4081 aatcgccagg atcccgctca cgccagagag tttttgcagc acacgagact gggcataagc
     4141 tgagaactcc agtccgccgt actctttttt gataatcatc gcgaagaagc gatgctcttt
     4201 aaggtacgcc cacaactccg gtggcagatc cgccagctca tgagtaatct ggaaatcatt
     4261 cgccatccgg caggcttctt ctaccgggcc gtcgagaaac gcttgctctt cagcggtcag
     4321 gcgcggctgc ggatagttat gcagcttttt ccagtccggc ttgccctgga acaagtcgcc
     4381 ctcccaccag gtggtgcccg catcaatcgc ttctttctca gtgcgcgaca tcggcggcat
     4441 caccttacgg aaaccgcgaa ataccggcgc ggaaatcatc gacttacgca taggcgcaaa
     4501 gttaaatggc acgaggataa tggtcagagg caccagtacc cacgccgacc acagaccagc
     4561 aacgccgagg gcggctgtcc aggcgagcaa aatcagactg ctgataaata agctcacgcg
     4621 gtgatagaac aacgcgccga gcaggacaac cgtagcgaga atactcaaaa tcatcataac
     4681 gaaaagcccc ttacttgtag gaggtctgac cacttgtgat gatatggttg tagtggatgt
     4741 aaaaacattt agcaatgtgt ttacaatata attacaacaa agctcacatt gttgctgttt
     4801 ttatccgcac ttcaggtcaa aaagtcctgg tcatagcacc tgcccgtact tctcgctttt
     4861 ggcggtatcc ggtacactgc attttgtcta ttacatttat gctgaaggat atcctcatgt
     4921 accaggatct tattcgtaac gaactgaacg aagcggcgga aacgctggct aactttttaa
     4981 aagatgacgc caatattcac gccattcagc gcgcggcggt cctgttagca gacagcttta
     5041 aagccggtgg caaagtgctt tcctgcggca acggcggttc ccattgcgac gctatgcact
     5101 ttgccgaaga gttgaccggt cgctaccgtg aaaaccgtcc gggctacccg gcgattgcta
     5161 tttctgacgt tagtcatatt tcctgcgtcg gtaatgattt cggtttcaat gatattttct
     5221 cccgctacgt tgaagcggta ggtcgcgaag gcgatgtact gctggggatc tccacctccg
     5281 gtaactctgc aaacgtgatc aaagcgatcg cagcggcgcg tgagaagggg atgaaagtga
     5341 tcaccctgac cggtaaagac ggcggtaaaa tggctggcac ggcggatatc gaaattcgcg
     5401 ttccgcactt tggttatgcc gaccgcattc aggagattca cattaaagtg atccatatcc
     5461 tgatccagtt aattgaaaaa gagatggtta agtaagtctg gcgtaggccg gataaggcgt
     5521 ttacgccgca tccggcactt gtgcgctgat gcctgatgcg atgctgacgc gtcttatcat
     5581 gcctacaaat ctgtacccga accgtaggcc gaataattcg tttacgccgc atccgacctg
     5641 aaaattctta aatcaatctt cgccggggtc catgcgctcc cgctgttgtg gaggttaccc
     5701 atgtgcgaat tgctcgggat gagcgccaac gtccctaccg atatctgctt tagtttcacc
     5761 ggacttgtac agcgtggtgg tggaaccggg ccacataaag atggctgggg cattaccttt
     5821 tacgaaggta aaggctgtcg cacatttaaa gatccacaac ccagctttaa ttcccccatc
     5881 gccaaacttg tccaggacta cccgataaaa tcctgttcgg tggtggctca tattcgccag
     5941 gctaatcggg gcgaggtggc gctggaaaat actcacccat ttacccgcga gttatggggg
     6001 cgtaactgga cttatgccca taacggacaa ctgacgggct acaaatcact ggaaaccggc
     6061 aacttccgcc cggtcggtga aaccgacagc gaaaaagcct tttgctggct gctgcataaa
     6121 ttaacgcagc gttacccgcg cacgccgggc aacatggcgg cagtgtttaa atatatcgcc
     6181 tcactggcgg atgaactgcg ggcgaagggc gttttcaaca tgctgctttc ggacgggcgc
     6241 tatgtaatgg cgtattgttc gactaattta cactggatca cccgccgcgc gccgtttggc
     6301 gtggcaacgt tgctggatca ggatgtggaa atcgacttca gctcgcagac cacaccgaat
     6361 gatgtggtca cggtgattgc gacacagccg ctgacgggca atgaaacctg gcaaaaaatt
     6421 atgccgggcg aatggcgctt attttgcctc ggggagcgtg tagtttgatg ccagttgtgg
     6481 ctgcaccact tcgtggctta acggcttgct gaccacgtaa cggccattgg ccacagaaac
     6541 ggtcggtggc ttacgggttt gctcaaagta gtcgtagccc ggcttcagct gctcccagaa
     6601 gtccttaaag ttggaatatt tatggcgctt catattggcg tcggtcatgc ggaatgggta
     6661 aatactcacc tgcacgctcg gctgaccaaa caccagcgca ccagtaacga actggaagat
     6721 ctcatcaata ccctgattgg tcattgcgta gcagccgatg gatacacaat cgccgtggat
     6781 catcaggtat ttcccttcat aaccatgcgc acggtcataa gcattgggga atccaatatt
     6841 aatcgctttg tagtaacggc tgtctggttt taactgatta cgctggacgc tataaaaccc
     6901 ttccgggctt ttgaaatcgc cctgacgctg ttttggccct aagccgccgg aatatttaca
     6961 gattttataa ctgtcgagca gttgatattg ctcgcccatt ttgacgtaaa gatcgagcgt
     7021 acgttcttcc ttgaagatct ggatgtagac aggggatccc atcaactgct gcttatactc
     7081 tttgctcact ggcgtggtcg agctactgct gcccagcaaa ccggcaaacg aaacgcacgg
     7141 gatcaacaac atcgcaagaa ttaatgcgat tttacgcata ctgcttattc cttgataaaa
     7201 cggttacaca cgccaggacg gcaaaatgga tcccaaatcg gaatagtctg gatttggaag
     7261 gctcacatta tcaccaaaag agttttacgc aagcctgtcg gcgctgggtt tacaaattta
     7321 gcacaggggc agttttcaat agtccg
//
LOCUS       DAZXUK010000195         7329 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZXUK010000195 DAZXUK010000000
VERSION     DAZXUK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.195"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="ACH3L8_003165"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="ACH3L8_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="ACH3L8_003166"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="ACH3L8_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFT4086169.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="ACH3L8_003167"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="ACH3L8_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="ACH3L8_003168"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="ACH3L8_003168"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFT4086170.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="ACH3L8_003169"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="ACH3L8_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFT4086171.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="ACH3L8_003170"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="ACH3L8_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFT4086172.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="ACH3L8_003171"
     CDS             complement(6510..7202)
                     /locus_tag="ACH3L8_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFT4086173.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1850 a         1773 c         1939 g         1767 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtgt tttgctgaaa
     7321 aaatgcgcg
//
LOCUS       DAZXUK010000196         7302 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZXUK010000196 DAZXUK010000000
VERSION     DAZXUK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.196"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            110..1009
                     /gene="lysO"
                     /locus_tag="ACH3L8_003172"
     CDS             110..1009
                     /gene="lysO"
                     /locus_tag="ACH3L8_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFT4086174.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1153..2805
                     /gene="hcp"
                     /locus_tag="ACH3L8_003173"
                     /gene_synonym="priS"
     CDS             1153..2805
                     /gene="hcp"
                     /locus_tag="ACH3L8_003173"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFT4086175.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            2817..3785
                     /gene="hcr"
                     /locus_tag="ACH3L8_003174"
     CDS             2817..3785
                     /gene="hcr"
                     /locus_tag="ACH3L8_003174"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFT4086176.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3918..5636
                     /gene="poxB"
                     /locus_tag="ACH3L8_003175"
     CDS             3918..5636
                     /gene="poxB"
                     /locus_tag="ACH3L8_003175"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFT4086177.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5673..6674
                     /gene="ltaE"
                     /locus_tag="ACH3L8_003176"
     CDS             5673..6674
                     /gene="ltaE"
                     /locus_tag="ACH3L8_003176"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFT4086178.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6685..>7302
                     /locus_tag="ACH3L8_003177"
     CDS             6685..>7302
                     /locus_tag="ACH3L8_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFT4086179.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFE"
BASE COUNT         1636 a         1925 c         1979 g         1762 t
ORIGIN      
        1 cccctctgat gcataacatt tccttttaat tgcaacttta ctcgcccttc cgctttcagt
       61 agtgtgtcat cgcgcatttc gacttcttct ttgcaaggac tggattttca tgttttctgg
      121 gctgttaatc attctggttc ccctgattgt gggttacctc attccgcttc gccaacaagc
      181 tgcgttaaaa gctattaatc agctattaag ctggatggtt taccttattc tcttttttat
      241 gggtatcagt ctggcgtttc tcgataacct cgccagtaac ctgttggcga ttctgcatta
      301 ttctgccgtc agtattaccg ttattttact gtgtaatatt gccgccctga tgtggctgga
      361 gcgaggcctg ccgtggcgca accaccatca gcaagaaaaa ctcccgtcgc gtattgcgat
      421 ggcgctggag tcgctaaaac tgtgcggcgt agtagtgatt ggttttgcca ttggtctgag
      481 tggactggct ttcttacaac acgcgaccga agccagtgaa tacacgttaa ttttgctact
      541 tttcctcgtt ggtattcagt tgcgcaataa tggcatgacc ttaaagcaga ttgtccttaa
      601 tcgccgggga atgattgtcg ccgtggtggt ggttgtcagt tcattaattg gtggtttaat
      661 taacgccttt attcttgatc tccccatcaa taccgcgctg gcaatggcct ccggtttcgg
      721 ctggtattct ctttccggta ttttattgac cgaatctttt ggtccggtaa tcgggagcgc
      781 ggcgtttttt aatgatctgg cccgtgaact gattgctatt atgttgatcc ctgggctgat
      841 tcgccgcagc cgctctactg cactgggctt atgcggtgcc acatcaatgg atttcaccct
      901 gcccgttctt caacgtactg gcgggctgga tatggtcccg gcggcaattg ttcacggttt
      961 tattcttagc ctgttagtgc cgatcctcat cgcctttttc tctgcgtaat acctctctgg
     1021 cggtagttcc ctgccgccaa aattgcgcta aatcaatctc ccttaaagtt gcatgaaaaa
     1081 tcccttttat ccccgcgtta agcgtcttac ccttaaacat gtatattaaa tataacttta
     1141 aaaggtgtga tcatgttttg tgtgcaatgt gaacaaacta tccgtactcc ggcagaaaac
     1201 ggctgctcat acgcgcaggg gatgtgtggt aaaacggcgg aaacttctga ccttcaggat
     1261 ttactcatcg cggcgctgca agggctttct gcctgggcgg taaaagcgcg tgaatacggc
     1321 atcatcaacc acgatgttga cagcttcgcg ccacgtgcct ttttctcaac cctgaccaac
     1381 gttaacttcg attctccgcg tattgtcggc tacgctcgtg aagcgattgc cttgcgcgag
     1441 gcgctgaaag cacaatgcct ggctgtagat gccaacgcgc gcgtcgataa cccgatggct
     1501 gacctgcaac tggtgagcga tgatctcggc gagctgcaac gtcaggcagc agaatttacc
     1561 cctaacaaag ataaagcggc gattggcgaa aacattctcg gcctgcgtct gctgtgcctg
     1621 tatggcctga aaggtgcggc ggcctatatg gaacacgcgc atgttctcgg tcaatacgac
     1681 aacgatattt atgcccagta ccataaaatc atggcgtggc tggggacctg gcctgccgat
     1741 atgaacgcgc ttcttgagtg ttcaatggaa atcggccaga tgaacttcaa agtgatgagc
     1801 attctggatg caggcgaaac cggtaaatac ggtcacccga cgccaaccca ggtcaacgtc
     1861 aaagcgacgg cgggtaaatg cattctgatt tccggtcacg atctcaaaga tctctacaac
     1921 ctgctggaac agaccgaagg cacgggcgtt aatgtctaca cccacggcga aatgctgcct
     1981 gcgcatggct acccggagct gcgtaaattc aagcatctgg tcggtaacta cggcagcggc
     2041 tggcagaatc agcaagtgga gttcgctcgt ttccctggcc ccatcgtgat gacctcgaac
     2101 tgcatcatcg acccaaccgt aggcgcttat gacgatcgta tctggacccg cagcatcgtt
     2161 ggctggcctg gcgtgcgtca tctggatggc gaagatttct ctgcggtcat cgcccaggcg
     2221 caacaaatgg cgggcttccc gtacagcgaa attcctcacc tgatcaccgt gggctttggt
     2281 cgccagacgc tgcttggtgc agccgatacg ctgattgatc tggttagccg tgaaaaactg
     2341 cgtcatatct tcctgcttgg tggctgtgac ggcgcacgcg gcgagcgcca ctacttcacc
     2401 gatttcgcca ccagcgtgcc ggatgactgc ctgatcctga ccctcgcctg tggtaaatat
     2461 cgctttaaca aacttgagtt tggcgatatc gaaggtctgc cgcgtctggt agatgcaggt
     2521 caatgtaacg atgcttactc agcaattatt ctggctgtca ctctggcaga gaaactgggc
     2581 tgtggtgtga acgatctgcc gctgtcgctg gtgctctcct ggtttgaaca gaaagcgatt
     2641 gtcattctgc tgacgctgct ttctctgggc gtgaaaaata tcgtcaccgg cccgactgca
     2701 cctggtttcc tgacaccaga cctgctggcg gtgctgaacg agaaattcgg cctgcgttct
     2761 atcaccactg ttgaagaaga catgaagcaa ctgttgaacg cgtaaggagt ttgtcgatga
     2821 cgatgccaac gaatcaatgc ccgtggcgga tacaggttca tcacattacg caagaaacgc
     2881 cggatgtgtg gacgatttcc ctgatttgcc acgatcacta cccatatcgc gccgggcaat
     2941 atgcactggt cagcgtgcga aactcagcgg aaacgctgcg tgcttacacc atttcctcca
     3001 cgccaggcgt gagtgaatat atcaccctga ccgtgcggcg gattgatgac ggtgtcggct
     3061 cccagtggct gacgcgcgat gtaaaacgcg gtgattatct ctggctttcg gacgcgatgg
     3121 gggaatttac ctgcgacgat aaagcagaag ataaattcct gttgctggcg gcaggctgcg
     3181 gcgtcacgcc gattatgtcg atgcgtcgct ggcttgcgaa gaaccgtcca caggccgatg
     3241 tgcgggtgat ctacaacgtg cgtacgccgc aggatgttat tttcgccgat gagtggcgta
     3301 actatccggt aacgctggtg gcagaaaata acgttaccga aggctttatc gctggtcgcc
     3361 tcactcgcga actgctggca gatgtacctg acttagcttc acgtaccgtg atgacctgcg
     3421 gcccggctcc gtatatggat tgggtagagc aggaagtgaa agcgctcggc gtgacgcgtt
     3481 tctttaaaga gaaattcttc accccagtag cagaagcggc gaccagcggt ctgaaattca
     3541 ccaaactgca accggcacga gaattttacg ccccggttgg caccacgcta ctggaggcgc
     3601 tggaaagcaa taacgttccg gttgtcgctg cctgccgtgc gggtgtttgc ggctgctgta
     3661 agacaaaagt ggtttccggt gaatatacgg tgagcagtac aatgacgctg accgacgccg
     3721 aaatcgctga aggttacgta ctggcctgct cctgccatcc gcagggggat ttggttctcg
     3781 cataatcgcc ttatgcccga tgatattcct ttcatcgggc tatttaaccg ttagtgcctc
     3841 ctttctctcc catcccttcc ccctccgtca gatgaactaa acttgttacc gttatcacat
     3901 tcaggagatg gagaaccatg aaacaaacgg ttgcagctta tatcgccaaa acactcgaat
     3961 cggcaggcgt gaaacgcatc tggggagtca cgggcgactc tctgaacggt cttagcgaca
     4021 gtcttaatcg catgggcacc atcgagtgga tgtccacccg ccatgaagaa gtggcggcgt
     4081 ttgccgctgg cgctgaagca caactttcag gagaactggc ggtctgtgcc ggatcgtgcg
     4141 gcccaggcaa cctgcacttg atcaacggcc tattcgattg ccaccgcaat aacgttccgg
     4201 tactggcgat tgccgctcat attccctcca gcgaaattgg cagcggctat ttccaggaaa
     4261 cccacccaca agagctattc cgcgaatgta gtcactattg cgagctggtt tccagcccgg
     4321 agcagatccc acaagtgctg gcaattgcta tgcgcaaagc ggtgcttaac cgtggcgttt
     4381 ccgttgttgt attaccgggc gacgtggcgt taaaacctgc gccagaaggg gcaaccacgc
     4441 actggtatca tgcgccacag ccagtcgtga tgccggaaga agaagagtta cgcaaactgg
     4501 cgcaactgct gcgttattcc agcaatatcg ccctgatgtg tggcagcggc tgcgcggggg
     4561 cgcataaaga gttagttgag tttgccggga aaattaaagc acctattgtt catgccctac
     4621 gcggtaaaga gcatgtcgaa tacgataatc cgtatgatgt tggaatgacc gggttaatcg
     4681 gattctcgtc aggtttccat accatgatga acgccgacac gttagtgcta ctcggtactc
     4741 agttccccta ccgtgcgttc tacccgaccg atgccaaaat tattcagatt gatattaatc
     4801 cagccagcat cggcgcgcat agcaaggtag atatggcgct ggtcggcgat atcaaatcaa
     4861 ccctgcgcgc attgctgcca ctggtggaag aaaaaaccga tcgcaagttt ctggataaag
     4921 cgctggaaga ttaccgcgat gcccgcaaag ggctggacga tttagctaaa ccgagcgaga
     4981 aagccattca cccgcaatat ctggcgcagc aaattagtca ttttgccgcc gatgacgcca
     5041 tctttacctg tgacgtcggc acgccaacgg tatgggcggc acgttatctg aaaatgaacg
     5101 gcaagcgtcg cctgttaggt tcgtttaacc acggttcgat ggctaacgcc atgccgcagg
     5161 cgttgggtgc gcaggcaacc gagccggaac gtcaggtggt cgccatgtgc ggcgatggcg
     5221 gttttagcat gttgatgggc gatttcctct cagtagtgca gatgaaactg ccggtgaaaa
     5281 ttatcgtctt taataacagc gtgctgggct ttgtggcgat ggagatgaag gccggaggtt
     5341 acctgacaga cggtactgag ctgcacgaca ccaactttgc ccgcattgcc gaagcgtgcg
     5401 gcattacggg tatccgtgta gaaaaagcct ctgaagttga tgaagccctg caacgcgcct
     5461 tctctatcga cggtccggtg ttggtggatg tggtggtcgc caaagaagaa ttagccattc
     5521 caccgcagat caaactcgaa caggccaaag gttttagcct gtatatgttg cgcgcaatta
     5581 tcagcggacg cggcgatgaa gtgatcgaac tggcgaaaac gaactggcta aggtaaaaag
     5641 ggtggcattt cccgtcataa taaggacatg ccatgattga tttacgcagt gataccgtta
     5701 cccgaccgag ccgcgccatg ctcgaagcga tgatggccgc cccggttggg gacgacgttt
     5761 acggagacga ccctaccgtt aatgctctgc aggactatgc agcagagctt tccggtaaag
     5821 aagccgccat ttttctgcct accggcactc aggccaacct ggtcgctctg ctcagtcact
     5881 gcgaacgtgg cgaagagtat attgtcggtc aggccgcgca taactatctg tttgaagccg
     5941 gtggcgcagc ggtgctgggc agtattcagc cgcaacccat cgacgcggct gccgacggca
     6001 cgctaccgct ggataaagtg gcgatgaaaa tcaaacccga cgatatccat ttcgcccgca
     6061 ccaaattact cagtctggaa aacacccaca acggcaaagt gctgccgcgt gaatacctga
     6121 aagaagcatg ggaatttacc cgcgagcgca atctggcgct gcatgttgac ggtgcgcgca
     6181 tctttaatgc cgtggtggct tacggctgcg aactgaaaga gatcacgcaa tattgtgatt
     6241 cgttcaccat ttgcctgtcg aaaggtcttg ggacgccagt cggttcatta ctcgtcggta
     6301 atcgtgatta cattaaacgt gccatccgct ggcggaaaat ggccggtggc gggatgcgtc
     6361 agtccgggat tctggctgcc gccgggatgt atgccctgga aaataacgtc gcacggttgc
     6421 aggaagatca cgacaacgcc gcctggatgg cggagcaact gcgtgaagca ggcgcggatg
     6481 tgatgcgtca ggacactaat atgctgtttg ttcgcgtcgg ggaagaaaat gctgccgcgt
     6541 taggcgaata catgaaagcg agaaacgtgc tgattaacgc ctcgccgatt gtccgcctgg
     6601 tgacgcatct tgacgtctcg cgcgaacaac tggcggaagt cgccgcccac tggcgcgcat
     6661 tcctggcgcg ttaaggagag agacgtgccg caacgcattt tagttctcgg tgccagtggc
     6721 tacattggtc agcatctggt gcgcacactc agccagcaag ggcatcagat cctagcggcg
     6781 gcacgtcatg tcgacaggct tgcaaagctg caactggcaa acgtcagttg ccataaagtc
     6841 gatctcagct ggccggataa ccttccggcc ctgttgcagg atatcgatac cgtctatttt
     6901 ctggtgcaca gcatgggcga aggcggcgat tttatcgctc aggagcgcca gctggctctc
     6961 aacgtccgcg atgcgctacg tgaagtacca gttaagcaat taatctttct cagttcgttg
     7021 caggccccgc cacatgagca gtcggatcat ctgcgagctc gtcaggctac ggcggacatt
     7081 cttcgtgaag cgaatgtacc agtgaccgaa cttcgggccg gaattatcgt tggcgcaggt
     7141 tcagcggcgt tcgaagtcat gcgcgatatg gtctacaacc tgccagtgtt aacgccgcca
     7201 cgctgggtac gttcacgcac cacgcccatc gcgctggaaa acttgctgca ctatctggtg
     7261 gcgctgttag accatccagc caacgaacac cgcatcttcg aa
//
LOCUS       DAZXUK010000197         7267 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZXUK010000197 DAZXUK010000000
VERSION     DAZXUK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.197"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1075
                     /locus_tag="ACH3L8_003178"
     CDS             <1..1075
                     /locus_tag="ACH3L8_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAPES1 domain-containing protein"
                     /protein_id="HFT4086180.1"
                     /translation="AEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTA
                     STDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPV
                     NVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVA
                     GQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYV
                     SYVHDLPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENI
                     SDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAIT
                     LHDAYKASDERLYVNKQNKNSRS"
     gene            1118..1621
                     /gene="yeaK"
                     /locus_tag="ACH3L8_003179"
     CDS             1118..1621
                     /gene="yeaK"
                     /locus_tag="ACH3L8_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFT4086181.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1622..1726)
                     /gene="yoaI"
                     /locus_tag="ACH3L8_003180"
     CDS             complement(1622..1726)
                     /gene="yoaI"
                     /locus_tag="ACH3L8_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFT4086182.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1896..2342
                     /locus_tag="ACH3L8_003181"
     CDS             1896..2342
                     /locus_tag="ACH3L8_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFT4086183.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2299..3117)
                     /gene="nimR"
                     /locus_tag="ACH3L8_003182"
                     /pseudo
     CDS             complement(2299..3117)
                     /gene="nimR"
                     /locus_tag="ACH3L8_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3214..4394
                     /gene="nimT"
                     /locus_tag="ACH3L8_003183"
                     /pseudo
     CDS             3214..4394
                     /gene="nimT"
                     /locus_tag="ACH3L8_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4449..4796
                     /locus_tag="ACH3L8_003184"
     CDS             4449..4796
                     /locus_tag="ACH3L8_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFT4086184.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4818..5072)
                     /gene="yoaF"
                     /locus_tag="ACH3L8_003185"
     CDS             complement(4818..5072)
                     /gene="yoaF"
                     /locus_tag="ACH3L8_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFT4086185.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5255..6280
                     /gene="dgcP"
                     /locus_tag="ACH3L8_003186"
     CDS             5255..6280
                     /gene="dgcP"
                     /locus_tag="ACH3L8_003186"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFT4086186.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6313..6411
                     /locus_tag="ACH3L8_003187"
     CDS             6313..6411
                     /locus_tag="ACH3L8_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFT4086187.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6414..6488
                     /gene="yoaJ"
                     /locus_tag="ACH3L8_003188"
     CDS             6414..6488
                     /gene="yoaJ"
                     /locus_tag="ACH3L8_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFT4086188.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6649..7026)
                     /locus_tag="ACH3L8_003189"
                     /pseudo
     CDS             complement(6649..7026)
                     /locus_tag="ACH3L8_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(7020..7136)
                     /locus_tag="ACH3L8_003190"
     CDS             complement(7020..7136)
                     /locus_tag="ACH3L8_003190"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4086189.1"
                     /translation="MRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
BASE COUNT         1753 a         1762 c         1770 g         1982 t
ORIGIN      
        1 tgctgagcag tcggaagtgt cgccagaaca gcggcgcgcc atctgcgagg cttttgagtc
       61 tgccaataat acccatggct taaacctgac tgcccataaa tacccgggct tacgcggcac
      121 actacaaacc gcatccactg actgcgacac aattgtggaa gctgcagcac tattacccgc
      181 ttttgatcag gcagtggaag gcaaccgcca ccaggatgat tacggttcag gtcttgggat
      241 ggccgaagag aaatttcact attatctcga tctcaatgac cgctatgtct atttttatga
      301 gccggttaat gttgaatact ttgcgatgaa taactggtcc ttcctgcagt caggaagtat
      361 tggcatcgat cgcaaagata ttgaaaaggt atttaccggg cgtaccgtat tgtcgagcat
      421 ttaccaggat cagcgtacta aacagaacgt gatgagtttg ctgacgccgg tatatgtcgc
      481 agggcagcta aaagggattg tgctgctgga tattaacaaa aacaatttgc ggaatatctt
      541 ttacactcat gaccgccctc tcctctggcg ttttctcaat gtcacgctaa ccgataccga
      601 ttcggggcgc gacattatca tcaaccagag cgaagataat ctgttccagt atgtcagtta
      661 cgtccatgac ttacctggcg gcattcgtgt ctcgttatcc attgatattc tttactttat
      721 cacgtcttcg aggaaaagcg ttctgttctg gattttgacg gcgttaattt tgctgaatat
      781 ggtccggatg cacttccgtt tataccaaaa tgtgtcgcga gaaaatatta gtgatgcgat
      841 gactggactg tataatcgca aaattttaac ccctgaactg gagcagcggt tgcagaaact
      901 ggtgcaatcc ggttcttcgg tgatgtttat tgcaattgac atggacaagt taaagcaaat
      961 aaatgacacc ctcggtcatc aggaggggga tttagcgatt acgttacatg atgcgtataa
     1021 agcttccgat gagcgtttat atgtcaataa gcagaacaaa aacagccgtt catgataacc
     1081 ttctgtggtt gtttgcttgt aatctcagga gcgtgaaatg actgaaatgg ctaaaggaag
     1141 cgtgacgcat cagcagttaa tcgctttatt atcacaagaa ggtgctaact tccgcgtcgt
     1201 tacccatgaa gcggtaggta aatgtgaagc ggtgtcagaa attcgtggta ccgctctagg
     1261 ccagggtgct aaggcactgg tatgtaaagt caaaggcaat ggcgtaaatc aacatgttct
     1321 ggcgatcctc gcagccgatc aacaggccga tctgagccaa ctcgccagcc atataggcgg
     1381 attacgcgct tcgttggcca gcccggcgga agttgatgaa ctcacaggct gtgtcttcgg
     1441 cgcgatcccc cctttcagct tccatccaaa actcaaactg gttgccgacc ctctactctt
     1501 cgaacgattt gatgaaatcg ccttcaatgc agggatgctg gataaatccg ttattttgaa
     1561 aaccgcggat tatttgcgca ttgcgcaacc agaactggtc aatttccgcc gcactgcgta
     1621 gctagcctat ccgttcaata agcagaacgg acaaaaccag cacaaccgca atagcaaaaa
     1681 acgatgatgt gataatcagt gtctcgacga acatttgatc gttcatagca tgcccccggg
     1741 agatagctgt tttcacgtta ttgttagcgt gcacaaatgg cagtttgatg acagttcgcc
     1801 tgatttttta tgcaaaaaac gtaaagattt tctacttcct tcctgcagca agcataaagt
     1861 aagcaggctt attatttttt ggcaaggaaa ccacgatgtt tgatgtcact ttgctgatcc
     1921 tgctcgggtt agctgcgctg ggctttatca gtcataacac cactgtcgcc gtttcaattc
     1981 tggtgttgat cattgtccgc gtcacaccgt taagcacctt tttcccgtgg attgaaaaac
     2041 aggggctaag tatcgggata atcattctga cgattggcgt tatggccccc atcgccagcg
     2101 ggacgctacc gccctcaacc ttgattcact cgtttctgaa ctggaaatcg ctggtggcaa
     2161 ttgcagtagg cgttattgtc tcctggctgg gtgggcgcgg cgtgacgtta atgggcagcc
     2221 agccgcaact ggttgctggg ctgctggtgg gcactgtttt aggcgtagcg ctgtttcgcg
     2281 gcgtaccggt tggcccgctt attgccgcgg gtctggtttc gctgattgtg gggaaacagt
     2341 agttaatccc gcaatatatc ggcctggcgt ttgccccaag cctttcttaa acatagtaat
     2401 aaaggccgtt gttgaatcat accccagcgt atgagcaact ttctgtacag tatcgccttt
     2461 cactaatccc tgaagcgcca taatcagctg taactgctga cgccactggc gaaaactcaa
     2521 ccccgtctct ttcactatca ggcgggcaag attgcgttca ctcatcgcaa aaaagccagc
     2581 ccattgcccc aatacccccc actcgacggg ccctttcgcc atcatctcca ccatcgtgcg
     2641 gattttagga tgagaagaaa cgggcaggtg cagctgttgt tgtggctgct gagggagttc
     2701 atcaaacagg acctgaatta atctgcgggt catcggctca gcacgctgag tcgtcgtcct
     2761 gtttgccagg gttaaaatca attcccggca taagggcgat attttcaatg tgcaacatgg
     2821 tcggcattgt cactgcgctg ggttcgataa acagaaaaca gagttcagcg ttagctgtca
     2881 cttgattgct atgcttcacc ccgcccggta tccaaaccgc atattgtggc ggcaccatcc
     2941 acaaagcatt ttccaccgta caggtaattg caccatgtaa tgccagtatc agctgtcctt
     3001 tgcggtgctg atgtacagga ctggttaact catccgttcc ggcgtgaata caaaatgcga
     3061 ccgcggcttc atggtgtcgg tgggttcata gccattgaga ttcaacctgt gcatcatttt
     3121 gtccgaactt agcgataatt tgtcatttta gcttgattca acataacaat aaaaacggta
     3181 aggtacagcc tcgtttgtaa caatgagaag catatgacct gttcaacttc attaagcggc
     3241 aaaaacagga ttgtccttat cgctggcatt ctgatgattg ccacaacatt acgcgtcacc
     3301 tttaccggcg cagcgccgtt actggatacg attcgttccg cttactcgct gacgacagcg
     3361 caaaccggct tattaaccac cctgccatta ttggcctttg cgctaatctc acctctggct
     3421 gccccggtgg cgcgacgttt tggtatggaa cgtagcctgt ttgccgcgtt acttttgatc
     3481 tgtgcaggta tcgcaattcg ttctctccct tcgccttact tattatttgg cggtacagcg
     3541 gtcattggcg gtgggattgc attaggcaat gtcttactgc caggattaat taaacgcgat
     3601 ttccctcatt ccgtcgccag acttaccggc gcatattccc tgacaatggg agctgcagcg
     3661 gcactgggat cggctatggt cgtgccgctg gctttgaacg gttttggctg gcaaggcgcg
     3721 ttgttcatgc tgatgtgttt tcctctgctg gctctttttt tatggctgcc acagtggcga
     3781 agtcagcaac atgcaaattt gagtacctca cgcgccttac atactcgggg tatctggcgt
     3841 tcgccacttg cctggcaggt aacattgttt cttgggatca actcactggt ctattacgtg
     3901 attattggct ggcttccggc gatcctcatc agtcacggct atagcgaagc acaggcgggt
     3961 tcactgcatg gtttgctgca actagccaca gcagctcccg gtttgctgat cgcacttttc
     4021 ttacatcatg tgaaagacca gcgtggtatt gcagcgttcg ttgccttgat gtgcgcagtg
     4081 ggcgcggttg ggctctgctt tatgccagcg cacgcgatca cctggactct gcttttcggt
     4141 tttggttccg gcgcaacaat gatactgggg ttgacgttca ttggtcttcg gcaagttctg
     4201 cgcatcaggc ggcggcactc tcggggatgg cacaatccgt cgggtatttg ctggcagcct
     4261 gtgggccgcc gctaatgggt aaaatacacg atgctaacgg taactggtcc gtaccactga
     4321 tgggtgttgc aatactttca ctactgatgg cgattttcgg actttgcgcc gggagagaca
     4381 aagaaattcg ctaatatccg gtgctatagt gacgtgacaa atcttgcgtg aaagggagaa
     4441 caaacacgat gaatattcag tgcaaacgcg tttatgatcc ggctgaacag agcgatggtt
     4501 atcgcgtact ggtcgaccgc ctctggccgc gcggtatcaa aaaaaccgat ttagcccttg
     4561 atgagtggga taaagaaatc acgccgtcaa cggaactgcg caaagccttt cacggcgaag
     4621 tcgtcgattt tgcaaccttt cgcgagcaat atcgcgcaga actggcgcaa cacgagcaag
     4681 aaggaaagcg gctggcggac atcgccaaaa aacagccgct gaccctgctc tacgcagcaa
     4741 aaaacaccac gcagaaccat gcgctggtgc tggccgactg gctacgtagc ttgtgatttt
     4801 agtacggcat ccggcggtta tttttcacca gccggatggt cccgtcgcca caatgcccat
     4861 tcatcaatcg tttcaccgcc cggtaatttg caattgttgc tgaccccttg cgctgtctgc
     4921 actggaatga gcgtcccgcc cttctgctgg caatagaccg acgccggatt tgccatacca
     4981 atctgcggcg gtttaggtgc ttctggctga gaaggggttg aacaaccagc caggaccagc
     5041 aagcaaggca gaacaaaact gataattttc atttattgat ctcacatatt tatccaagct
     5101 tagagtatcg cggtatcgtt ttgttttgca gcactatttt tattacattc actcaaaaca
     5161 tattacgtct tgtttcatct ttgttgatga tgttttatca tgcctgcaaa gattaaataa
     5221 tcagcactta cccgccgtat cctggagttg ttccgtgtca gatcagatta tcgcccgcgt
     5281 ctcgcaatcc cttgccaaag aacagtcact ggaaagtctg gtccgacagc ttctggagat
     5341 gctggaaatg gtcactgata tggaatcaac ctacctgacc aaagtggatg tcgaagcgcg
     5401 cctgcagcat attatgtttg cccgtaacag ccagaaaatg cacatcccgg agaattttac
     5461 cgtcttgtgg gattactcgt tatgcaaacg cgccattgat gaaaactgct ttttcagcga
     5521 tgaagtccct gaccgttggg gcgactgtat tgcggcacgc aatcttggca tcaccacatt
     5581 tctgagcacg ccaattcact taccggatgg atcattctat ggcacgcttt gcgccgccag
     5641 cagtgagaag cgccagtgga gtgaacgcgc ggaacaggtt ttacagttat tcgccggact
     5701 gattgcacaa tatattcaaa aagaggcact ggttgaacag ctgcacgaag ccaatgctgc
     5761 gctgattgcg caatcgtata ccgactcgtt aaccgggcta ccgaatcggc gggcgatttt
     5821 tgaaaatctg acgacgctgt tttccctcgc ccggcatctt aaccataaga taatgatcgc
     5881 gtttatcgat ctggataact tcaaattaat caatgatcgt tttggtcata atagtggcga
     5941 tctgtttctc attcaggttg gcgagcgcct taatacgctc cagcaaaatg gcgaagttat
     6001 tggtcgtctc ggtggtgatg agtttttggt tgtttcacta aacaacgaga atgcggatat
     6061 ttcgtcgctg cgagaacgta ttcaacagca aatacgtgga gaatatcact taggtgatgt
     6121 tgatttgtat tatcccggtg ccagtcttgg catagtagaa gtcgatcctg aaacaaccga
     6181 tgcagacagt gccctgcatg ctgccgatat tgcgatgtat caggagaaaa aacacaaaca
     6241 gaaaacacct tttgtcgcgc atccagcgct acattcctga ggcgtattca catccttttg
     6301 attggtgata acatgcgaat cggtattatt tttccggttg taatcttcat tacagcggtc
     6361 gtatttttag catggttttt tattggctgc tatgctgccc cgggagcata aagatgaaaa
     6421 aaacaacgat tattatgatg ggtgtggcga ttattgtcgt actcggcact gagctgggat
     6481 ggtggtaacg tcacctctaa aaaatagcaa aggctgcctg tgtgcagcct ttgtgcaatt
     6541 taagcgttaa cttttaatct tcctgtagat aaatagcacg acaatcgcac caataacggc
     6601 tgaagcggcg cacgaaaaac gctttcgcgt ttttcgtgcg ccgcttcacc cctggttcag
     6661 catttcgtct gcagtctgaa ccgcaggcaa ccatttttca gcaagtctgc gctgggaatc
     6721 gctggcttta tcccatttgc tccacagtgc ctcacgatcg taatcgtgcg cttccagcgt
     6781 ggggcgggcg atttcaaaat acccctagct tgtttctatt tgctcagatt ttaataacaa
     6841 ctcagcacgt gctgccgctg gtagaactga tatatgaaat tccaaagcct ttgaacccgt
     6901 acgcttgcga cgtagttctg gatgattggc tgcaagcttt tcaagattgc ggcgaatact
     6961 cacctcatgt ttaggaaagc atgacattcc tacgcattct tgcgctgtta cccaatgggt
     7021 taacatgatg ttttctccat ataccgagaa ggccaaatct gggctggttc catatccaaa
     7081 tatctagcaa tgattgcttc gtatcgtggt atatggcagc gaaatacgtt acgcatagtg
     7141 tcaggcttta agcctgcctt gaggtgtact ggcaatagcg gatactacca tttgttcttt
     7201 ttttaagcag ttatctgatg atatttttcc ctgaaggctg ccggggagat attccccaga
     7261 cgagagt
//
LOCUS       DAZXUK010000198         7216 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZXUK010000198 DAZXUK010000000
VERSION     DAZXUK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.198"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(83..487)
                     /locus_tag="ACH3L8_003191"
                     /pseudo
     CDS             complement(83..487)
                     /locus_tag="ACH3L8_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            complement(671..2089)
                     /gene="gltD"
                     /locus_tag="ACH3L8_003192"
     CDS             complement(671..2089)
                     /gene="gltD"
                     /locus_tag="ACH3L8_003192"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFT4086190.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(2102..6562)
                     /gene="gltB"
                     /locus_tag="ACH3L8_003193"
     CDS             complement(2102..6562)
                     /gene="gltB"
                     /locus_tag="ACH3L8_003193"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFT4086191.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
BASE COUNT         1581 a         1989 c         1938 g         1708 t
ORIGIN      
        1 ccttcgttta tttgcatgaa tatacaaacc gaaaatagga gatgcaatac aaaagctgtg
       61 gaaatatcaa acagcctctc aatttggcag acagcgtctg ccaaatcttt gccgatggga
      121 attctgcaga tggtgtctgc ggaattccca atggtgttaa ttcgccagac actgtctggc
      181 gaatttcaac atgttgaaaa aagaggtaaa aaagcctcat ttggcgtaag ttttttgcag
      241 agaccccacg tttataccgt cgacttaaat ccttcgatag ccgatcaatc aactgtgcac
      301 cataagctgc tctggcctca ccaccttgtt caaattcgac aatccgtcgg ccaatttccc
      361 agtacgtcgc ggtcatcagc gcgttaacgc tacgtaccgt ttccgttcgg gcgctatcga
      421 ccagatgaat aatgccgttg tggatttgct ggtagcctgt tgaggttctc tctgagagcg
      481 attccattat ctgtcccttt atatggcgct gcgttgaata atggaatcag ctatacgccg
      541 ggcatctacc ggtaacaatc cagatgagcg tattacattg gttttcttca gattatgcga
      601 ggcgatttcc agaaagaaag gagaatttca gcgccgactc ttttctgagg aggcggcgct
      661 gggacagagc ttaaacttcc agccagttca taatgccgtc tgccgctttg cgaccttcgg
      721 cgatggcggt aacgaccaga tcggaaccac ggacgatatc gccgccagca aagattttcg
      781 ggttgctggt ctggaaagcg ttgtcgttgc cttccggagc gatgatgcgg ccttgtgaat
      841 ccagctcgac gctgtgtttt gccagccatt ccatgttgtg tggacggaaa ccaaacgcca
      901 tgatcaccgc atctgccgga acgatatgtt cggaacctgc aacgatctcc gcgcggcgac
      961 ggcctttggc atccggttcg cccatttcgg tacgtaccat ttttacgccg ctgactttgc
     1021 cgttaccgtt cacttcaatc cccagtggct ggacgttgaa tttgaactct acgccttctt
     1081 cccgcgcgtt tttcacttcg cggcgggaac ccggcatgtt ctcttcatca cgacgatagg
     1141 cacaggtaac gtgctttgcc ccctggcgta cagacgtgcg cacgcagtcc atcgcagtgt
     1201 cgccaccgcc aagtactacc acgcgtttgc cttccatgct gacgaacggt tcgtcgcggg
     1261 tttcaccaaa gcccattaac tgtttggtgt tggcgataag gaacggcagc gctgcgtaca
     1321 cgccatcggc gtcttcgttt tccagcccgc cgcgcattga ctgataagtc ccaacgccaa
     1381 ggaacacggc atcgtaatca ctcagcagat cgtccagctg tacgtcgcgg cccacttcgg
     1441 tattgagttt gaattcaata cccatgccgg tgaagatttc acggcgacgc gtcattacct
     1501 ctttttccag cttgaaggcc ggaataccga aggtcagcag cccgccaatt tctggatgac
     1561 ggtcgaagac aacggctttt acgccgttac gcgtcaggac atccgcacac gccagacctg
     1621 ccgggcctgc accgataatc gccacttttt taccggtttg cttcacgccg gacatatccg
     1681 gacgccagcc catttcgaac gctttatcat tgatataacg ctcaatgttg ccgatggtca
     1741 ccgcaccgaa ctcatcgttc agggtgcagg aaccttcgca cagacggtct tgcgggcaga
     1801 cacgtccgca tacttccggc agtgtgttgg tctgatgcga cagttctgcc gcttcaaaaa
     1861 tacgcccctc gttggcgagc ttcagccagt ttgggatgta gttgtgtacc gggcatttcc
     1921 actcgcagta tgggttgccg cacgacagac agcggtcagc ctgcgctttg gcctggcctt
     1981 cggaaaacgg ctcgtaaatt tcaacaaact caattttacg gatcttcagc ggtttctttg
     2041 gcggatcaac gcgctgcagg tcgataaatt gataaacatt ctgactcatt gttgctaccc
     2101 cttactgcgc ctgcacgcgc aactctgctg cgctacgact acggtgaccc agcagtgctt
     2161 ttacatcact ggacttcggt ttaaccagcg cgaatttcgt ggcgaaggtt gaccagttcg
     2221 ccagaatctc ttcaccgcgc tgagaaccgg tatgctgaac atgctcggtg ataagaccgc
     2281 gcagatgctc ttcatggatc gccagatcgt cgacgcttaa gacttcgacc agttccgggt
     2341 taacgcgttt gcggaaatca ccgctttcat cgagaacgta agcgaagccg ccggtcatgc
     2401 ccgcgccgaa gttaacgccg gttttaccca gaatacagac aataccgccc gtcatatatt
     2461 cacagccgtt gtcgccaatg ccttctacca cggtgatagc accggagttc cgcacgccga
     2521 aacgttcacc cgcgcggcct gcggcataca gacgaccacc ggtcgcgcca tacaggcagg
     2581 tgttgccgat aatgcttgct tcatggctgc ggaaggcgga accaaccgga ggacgaatgg
     2641 cgattaagcc gcccgccatg cctttaccga catagtcgtt ggcatccccg gtcaggtaca
     2701 gttccacgcc gcccgcgttc cacacaccga aactctggcc tgcggtgccg ttgaagtacg
     2761 ctttgatagg atcggctgcc agcccctgat cgccgtgcgt ctgggcgata tagcctgaaa
     2821 gcgacgcgcc gacagaacgg tcggtgttgc ggatatcgaa ccagaaggtt ttgctctggc
     2881 gttcatcgac aaacggtttc gcctgttgca gcaactgcgc gttcagtagg ccgttatcaa
     2941 acggcgggtt gttttcagtg cagtagagtg acttacctgc atgtggctcg gcagtctcca
     3001 gcagcttcga cagcgccagt ttctgctgtt tggcggtgaa accgtccagc tctttcagca
     3061 ggtcggtgcg accaatcaga tccaccagac gcgttactcc cagctgtgcc atcagctcgc
     3121 gggtttcacg ggcgataaac tcaaagtaat tagtcacctt gaacggcagg ccgtgatagt
     3181 gattcttacg cagtttgtca tcctgagttg ctacacccgt ggcgcagttg ttcaggtggc
     3241 aaatacgcag atatttacag ccgagcgcca ccattgggcc agtgccgaag ccgaagcttt
     3301 ctgcgccgag aatcgccgct ttgatgatat cgacacccgt tttcaggccg ccatcaacct
     3361 gcaaacgaat tttatgacgc aagccgttgg caaccagcgc ctgttgggtt tccaccagac
     3421 cgagttccca cgggcagcct gcatatttca ctgacgacag cgggcttgcg ccggtgccgc
     3481 cgtcatagcc cgcgatggtg atcaagtccg cataagcttt cgccacgcca gtcgcgatgg
     3541 tgccaacgcc tggttcggac accagcttaa cggagatcat cgctttcggg ttaacctgct
     3601 tgaggtcgaa aataagctgc gctaagtctt cgatagagta gatatcgtgg tgcggcggcg
     3661 gggagatcag cgtcactccg ggcaccgaat agcgcagttt ggcgatgtaa ggagtgactt
     3721 tgtcgcccgg caactgacca ccttcgcccg gcttcgcgcc ctgggcgact ttaatctgaa
     3781 tgacgtcggc attgaccaga tacgccggag taacgccgaa gcgaccagaa gccacctgct
     3841 tgatgcgcga cactttgtta gtgccatagc gcgccgggtc ttcgccgcct tcaccggagt
     3901 tcgagttacc gccgatgcta ttcatcgctt ccgccagcgc ctcgtgggct tccgggctta
     3961 acgcgccgat agacatcgcg gcggtatcaa agcgtttaaa cagttcgctt gccggttcaa
     4021 catcagcaat gttgaccgcg ttttcacccg gcgtaattgc cagcagatcg cgcagcgtgg
     4081 ttgccggacg ctcattaacc agcttcgcgt attcctgata gtcgctgtac tcgccgcttt
     4141 gtaccgcctg ttgcagcgtg cgcaccacgt ccgggttgta ggcgtggtat tcgccgccgt
     4201 ggacgtattt cagcaggccg ccctggctaa tcggcttgcg cgccagccag gcacgtttcg
     4261 acagattcag cagatcctgc tggaagtctt caaagcttgc cccaccaatg cggctgaccg
     4321 cgccctggaa gcacaggccc actacatcat cgtgtagacc gaccgcttca aacagtttcg
     4381 agcagcggta agaggcgatg gtggagatgc ccattttgga catgattttg tacaagcctt
     4441 tgttgatgcc gttacggtag ttgagcatca cggtacgata atctttggca atcgcgtggg
     4501 tgtctaccag gcggcccagc gtttcatagg caaggtacgg ataaatagcc gtcgcgccga
     4561 aacccagcaa cacggcgaag tggtgcggat cgcgggcgct ggcggtttcg acgatgatgt
     4621 tggcatcgca acgcaggctt tgatcgacca gacgggtctg gatcgcgcca accgccatcg
     4681 gagccggaac cggcaggcga tctttagcga tattccggtc ggagagcacc agcaacacgg
     4741 tgccgctacg taccattttt tcggctttgt cgcatagctc tttgactgtc gcttcgagcg
     4801 tggttttagt gacgtcaaag gtgatatcca gcgtatctgc gcggtagtgc tcctctttca
     4861 tcgtcgtgag ctgtttgaaa tcggagtaga gcagaatcgg cgatttaaag cttaaacggt
     4921 gcgcctggcc ctctgcttcg caaaagacgt tcatttcacg accgatactg gtggcgagcg
     4981 acataacatg cgcttcacgc agcgggtcga ttggcgggtt agtcacctgg gcaaactgct
     5041 ggcggaagta gtcgtaaata atgcgcggct gactggagag cacggcgaat ggggtatcat
     5101 cgcccatcga accgaccgct tcctgaccgt tttcgcccag tacgcgaatt acggagtcca
     5161 gctcttccgc gctgtagtta aactgtttct ggtagctggc aagcgtgtcg tcgtccagtt
     5221 cgcggctacc cacttcttca tcgggcagat cttcaaacgg taccagtcgg cggacgtttt
     5281 tctccatcca ctctttataa gggtggcggc ttttcagatc gtcatcggtt tctgccgagt
     5341 gcagaatacg cccactgcga gtgtcgatca ccatcagttc gcctggcccg acgcggcctt
     5401 tttcgaccac ttcgtcaggc tggtaatccc agataccgac ttcagaggcg caggtgatga
     5461 gcttgtcttt ggtaatgacg tagcgtgccg gacgcagacc gttacggtcg aggttacagg
     5521 cagcgaagcg accatcggac atcacgatgc ccgccggacc atcccacggc tccatatgca
     5581 tggagttaaa gtcaaagaag gcacgcagtt ccgggtccat atccgggttg ttctgccagg
     5641 cgggtggcac taacaggcgc atggcgcgga tgatatccat cccgcctgcc agcagcagtt
     5701 ccagcatgtt atccatggaa ctggagtcag aaccggtttc gttgacgaat ggcgcggcgt
     5761 cgtgcaggtc agggatcagc ggcgtctgga atttataggt acgcgcacgc gcccattggc
     5821 ggttaccggt gatggtgttg atttcaccgt tatgcgccag atagcggaac ggttgcgcca
     5881 gcggccagcg cggtacggtg ttagtggaga agcgctggtg gaacaggcaa atggccgatt
     5941 ccagacgcag gtccgcaaga tccagataaa agcgcggcag atccgccggc atacacagac
     6001 ctttatagat gttcaccaga ttcgacaggc tacagacgta gaagtctttg tcggcttcga
     6061 gacgcttttc aatgcggcgg cgggcgataa acagacggcg ctccatatcg cgtggacgcc
     6121 agcctgccgg ggcgttcaca aaaatttgct caatgcgtgg cagagaggag agggcgattt
     6181 cacccagcac gccttcgtta gtggggacat cacgccagcc cacaatcgac aaggtttcgc
     6241 gttgcagttc ttcttcaacg atgcggcgtg cggcagcggc gagttcagga tctttattca
     6301 ggaagagcat cccgacagcg tagttttttg ctaaacgcca gccgcgctcc tgcgcaacga
     6361 tgcgaaaaaa gcgatccggt ttttgtaaca gcaagccgca accgtcgccg gttttaccat
     6421 cggcgagaat cgcgccacgg tgctgcatgc gggccagtgc gtgtattgca gtacgcacta
     6481 ccttgtggct aggttcgcct tctatgtggg cgatcaggcc gaaaccacag ttatccctct
     6541 caagggattt atcgtacaac atatcgtgaa cctcccccag gctctgcggg aaccccactt
     6601 ccgaaccgct gcgcacgggc acagaaagag cgtggcgacg gggtttgcgt gtcatacgcg
     6661 cgcctcgcat tcgccccctt gttgtccttt tcgcatcggt taatacggtg aagaggggat
     6721 ctcaattact gcataaatat gatgagcagg ctgctcatcc agaaagcttc cagcggattt
     6781 ccaacttatc gggaaagctg atacaggtca aatggcaagc ttattggtac agaaatgtgc
     6841 tttagcgcaa aattctttat taaatagctg aaattaaaat agattttatc tttaaggcta
     6901 aaaggagagt cgttagagaa acagtcaatt cctgactgtc ataaaggcgt gttaaccctt
     6961 atcctgcctt gaactgacga gaaaaccagc attttatact gccttaattg gtaatgacgt
     7021 ttcatgctac tgtttttgcc taaaatccat aaggagataa aaaaatctga cacaagataa
     7081 gcaaaagtga tttatgtgat tgtgcatgaa atttcggtaa ttttgattga ttatgcagtt
     7141 gataaaagca ggccgggacg attgatccag gtcatcgccg aattgagcta aaaatggcag
     7201 gctttggcct cttttc
//
LOCUS       DAZXUK010000199         7183 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZXUK010000199 DAZXUK010000000
VERSION     DAZXUK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.199"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(371..1198)
                     /locus_tag="ACH3L8_003194"
                     /pseudo
     CDS             complement(371..1198)
                     /locus_tag="ACH3L8_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACH3L8_003195"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACH3L8_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFT4086192.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="ACH3L8_003196"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="ACH3L8_003196"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFT4086193.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="ACH3L8_003197"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="ACH3L8_003197"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFT4086194.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="ACH3L8_003198"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="ACH3L8_003198"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFT4086195.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="ACH3L8_003199"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="ACH3L8_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="ACH3L8_003200"
     CDS             7090..>7183
                     /locus_tag="ACH3L8_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HFT4086196.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1675 a         1812 c         1876 g         1820 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaatt
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAZXUK010000200         7171 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZXUK010000200 DAZXUK010000000
VERSION     DAZXUK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.200"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1158
                     /locus_tag="ACH3L8_003201"
     CDS             <1..1158
                     /locus_tag="ACH3L8_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFT4086197.1"
                     /translation="IWNWEISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPT
                     YFGDTDGSVGAVLYALFATGHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTN
                     VNTIISILNRRDNVLESLQPYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKR
                     IMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMAN
                     HHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAIS
                     CRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARD
                     SKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1348..1461
                     /gene="yhiY"
                     /locus_tag="ACH3L8_003202"
     CDS             1348..1461
                     /gene="yhiY"
                     /locus_tag="ACH3L8_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFT4086198.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1524..2591
                     /locus_tag="ACH3L8_003203"
     CDS             1524..2591
                     /locus_tag="ACH3L8_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFT4086199.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2588..5323
                     /gene="rbbA"
                     /locus_tag="ACH3L8_003204"
     CDS             2588..5323
                     /gene="rbbA"
                     /locus_tag="ACH3L8_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFT4086200.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5323..6447
                     /locus_tag="ACH3L8_003205"
     CDS             5323..6447
                     /locus_tag="ACH3L8_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4086201.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6506..6781
                     /locus_tag="ACH3L8_003206"
     CDS             6506..6781
                     /locus_tag="ACH3L8_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFT4086202.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            6778..7137
                     /locus_tag="ACH3L8_003207"
     CDS             6778..7137
                     /locus_tag="ACH3L8_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFT4086203.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
BASE COUNT         1737 a         1750 c         1971 g         1713 t
ORIGIN      
        1 atctggaact gggaaatatc gcaatacaat gtatttgata ttaaattcga aacaattata
       61 aaaccccagt tcgctgacag aacctatttc tccaattgtt cacctgttga tcccacaagt
      121 accaggccga cctattttgg cgataccgat ggctcagtcg gtgcagtttt gtacgcgctt
      181 tttgcaacag gacatttggg aatcatggct gaaggagaaa atttccttag ccagttgctt
      241 aatatagaag atgaagttct caacgtattg ctaagggaga actttaatga gcaacttgac
      301 actaatgtta atacaataat cagtatactg aacagaagag ataatgtttt agaaagtctt
      361 cagccttatt tggttattaa taaagacgca gtcacgccat gtacgtttct tggcgaccaa
      421 accggcgatc gatttagtaa tatctgtggc gatcaattta tcatcgattt gttaaaacgc
      481 ataatgagta tcaatgaaaa tgttcatgta ttagctggaa atcatgaaac gaattgcaat
      541 ggtaactata tgcaaaactt cacgcgcatg aaaccacttg atgaggatac ctacgacggg
      601 ataaaagatt atcctgtatg tttctatgac cccaaataca aaataatggc aaatcatcat
      661 gggataactt ttgatgatca gcgaaaacgt tacattatag gaccgataac tgtctctatt
      721 gatgaaatga ccaatgctct cgatccggtc gaattagcgg aaattattaa taaaaagcat
      781 catgctataa ttaatggcaa aaagtttaaa actagccggg ctatctcttg tcgatccttt
      841 aatcgttatt tttctgtttc gacagattac agacctaaac ttgaagcact attagcatgt
      901 tcacaaatgt taggtataaa tcaagttgtt gcacataacg gaaatggtgg gagagaacgc
      961 ataggtgaaa cggggacagt tttaggtctt aacgcccgtg acagtaaaca tgctggaaga
     1021 atgttcagca tgcataattg ccaaattaac cctggtgcag ggccagagat aaccactccc
     1081 tggaagtctt accagcatga aaaaaacaga aatggactta tgcctttgat tagaaggcgc
     1141 acaatgttac agctctgaac catagacaac atgaatatta atgttactat ctggttgaga
     1201 ctgttgataa aacgtaaaaa ggaatgctct gttcctgcaa ctgaacaggc gtgaaatgcg
     1261 atccaacagg atggataccg ttttgtcagc cagttttata gatcacactt atgagtatgc
     1321 gtgtttcatt tacggccagg aatgctgatg atgacaacat tactacccgt tttcactaag
     1381 ccttccccgt tggcgctcaa tgctctgcgc gctggtcgta tttgtcgttt ccttcttatc
     1441 ccggatggaa gaatccgctg aacgtcgttc ggcggcagtt gtaacacgcg tcgtttgctt
     1501 acagggatta tggcgggtcg attatggata agagtaagcg ccatctggcg tggtgggttg
     1561 tcggggcact ggcggtggct gccgtcgtgg cgtggtggct gttgcgcccg gcgggtgtgc
     1621 cggaaggctt tgctgtcagc aatgggcgca ttgaagcgac ggaagtggat atcgccagca
     1681 aaattgccgg gcgtatcgac accattctgg tgaaagaagg ccagtttgtt cgcgaaggcg
     1741 aagtgctggc gaagatggat actcgcgtgt tgcaggaaca gcgactggaa gccatcgcgc
     1801 aaatcaaaga ggcacaaagc gccgttgccg ccgcgcaggc tttgctggag caacgacaaa
     1861 gtgaaactcg tgccgcacag tcgctggtca atcaacgcca ggccgaactg gactctgttg
     1921 ccaaacgtca tacgcgttcc cgctcgctcg cccaacgagg ggctatttct gcgcaacagc
     1981 tggatgacga ccgcgccgcc gctgagagcg cccgagctgc gctggaatca gcgaaagccc
     2041 aggtttcggc ttctaaagcg gctatagaag cagcacgcac caatatcatt caggcgcaaa
     2101 ctcgggtcga agcagcgcaa gccactgaac ggcgcattgc cgcagatatc gatgacagtg
     2161 aactgaaagc cccgcgtgac ggacgcgtgc agtatcgggt tgccgagcca ggggaagtgc
     2221 tggcggcagg cggtcgggtg ctgaatatgg tcgatctcag cgacgtctat atgaccttct
     2281 tcctgccaac cgaacaggcg ggcacgctga aactgggcgg tgaagcccgg ctgatcctcg
     2341 atgccgcgcc agatctgcgt attcctgcaa ccatcagttt tgtcgccagt gtcgcccagt
     2401 tcacgccaaa aaccgtcgaa accagcgatg aacggctgaa actgatgttc cgcgtcaaag
     2461 cgcgtatccc accggaatta ctccagcagc atctggaata tgtcaaaacc ggtttaccgg
     2521 gcgtagcgtg ggtgcgggtg aatgaagaac ttccgtggcc tgacgacctc gtggtgaggt
     2581 tgccgcaatg acgcatctgg aactggttcc cgtcccgcct gtcgcgcaac tggcgggcgt
     2641 gagccagcat tatggaaaaa ccgttgcgct gaacaatatc actctcgata ttccggcccg
     2701 ttgcatggtc gggctgattg gtccggatgg cgtcgggaag tcgagtttat tgtcactgat
     2761 ttccggtgcc cgcgtcattg agcagggcaa tgtggtggtg ctgggtggcg atatgcacga
     2821 cccgaagcat cgccgagacg tctgcccgcg catcgcctgg atgccgcagg ggctgggcaa
     2881 aaacctctac cacaccttgt cggtgtatga aaatgtcgat tttttcgctc gcctgttcgg
     2941 tcacgataaa gcggagcggg aagcgcgaat taatgagctg ctgaccagca ccgggttagc
     3001 accgtttcgc gatcgtccgg caggtaaact ctccggcggg atgaagcaaa aacttgggct
     3061 gtgctgcgcg ttaatccacg acccggaact gttgatcctt gatgagccaa caacgggggt
     3121 tgacccgctc tcccgcgccc agttctggga tctgatcgac agtattcgcc agcggcagag
     3181 caatatgagc gtgctggtcg ccaccgccta tatggaagag gccgaacgct tcgactggct
     3241 ggtagcgatg aatgccggag aagtgctggc aaccggcagc gccgaagagc tgcggcagca
     3301 aacgcaaagc gccacgctgg aagaagcatt tattaatctg ttaccgcaag cgcaacgcca
     3361 ggcgcatcag gcggtagtga tcccaccgta tcaacctgaa aacgcagaga ttgccatcga
     3421 agcgcgcgat ctgaccatgc gttttggttc cttcgttgcc gttgatcacg tcaatttccg
     3481 cattccacgc ggggagattt ttggattcct tggttcgaac ggctgcggta aatccaccac
     3541 catgaaaatg cttaccggac tgctgcccgc cagcgaaggt gaggcgtggc tatttgggca
     3601 accggttgat ccaaaagata tcgatacccg ccgtcgtgtg ggctatatgt cgcaggcgtt
     3661 ttcgctctat aacgaactca ccgtgcggca aaacctggag ttacatgccc gtttgtttca
     3721 catcccggaa gcggaaattc ccgcgcgagt ggctgaaatg agcgagcgtt ttaagctcaa
     3781 cgacgttgaa gatgttctgc cggagtcatt gccgctcggc attcgccagc ggctttcgct
     3841 ggcggtggcg gtgattcatc gcccggagat gttaatcctc gatgagccta cttctggtgt
     3901 cgatccggtg gcgagggata tgttctggca gttgatggtc gatctctcgc gccaggacaa
     3961 agtgactatc ttcatctcca cccactttat gaacgaagcg gaacgttgcg accgcatctc
     4021 actgatgcac gccggaaaag tgcttgccag cggtacaccg caggaactgg ttgagaaacg
     4081 cggagccgcc agtctggaag aggcatttat cgcctatttg caggaagcgg cagggcagag
     4141 caacgaagcc gaagcgccgc ccgtggtaca cgacaccacc cacgcgccgc gtcagggatt
     4201 tagcctgcgc cgtctgttta gctacagccg ccgcgaagcg ctggaactgc gacgcgatcc
     4261 agtacgttcg acgctggcgc tgatgggaac ggtgatcctg atgctgataa tgggttacgg
     4321 catcagtatg gatgtggaaa acctgcgctt tgcggtgctc gaccgcgacc agaccgtcag
     4381 tagccaggcg tggacactca acctctccgg ttcccgttac tttatcgaac agccgccgct
     4441 caccagttat gacgagcttg atcgtcggat gcgtgcgggc gatatcacgg tggccattga
     4501 gatcccgccc aatttcgggc gcgatatcgc gcgtggtacg cctgtggaac tcggcgtctg
     4561 gatcgacgga gcgatgccca gtcgtgccga aacggtaaaa ggttacgtgc aggcaatgca
     4621 ccagagttgg cttcaggatg tggcgagccg acaatcgaca cccgccagcc aaagcgggct
     4681 gatgaacatt gagacgcgtt atcgctataa cccggacgta aaaagcctgc cagcgattgt
     4741 tccggcggtg atcccgcttc tgctgatgat gatcccgtca atgctaagcg cccttagcgt
     4801 ggtgcgggaa aaagagctag ggtcgattat caacctttac gtgaccccca ccacccgtag
     4861 cgaatttttg cttggcaaac agctgccgta catcgcgctg gggatgctga actttttcct
     4921 gctctgcggc ctgtcggtgt ttgtgtttgg cgtaccgcat aaaggcagtt tcctgacgct
     4981 caccttggcg gcgctgctgt atatcatcat tgccactgga atggggctgc tgatctccac
     5041 ctttatgaaa agccagattg ccgccatttt cggaacggcg attatcacat tgatcccggc
     5101 gacacagttt tccgggatga tcgatccggt agcttcgctg gaagggcctg gacgttggat
     5161 cggcgaggtt tacccgacca gtcattttct gactatcgcc cgcggaacgt tctcgaaagc
     5221 gctggatctg actgatttgt ggcaactttt tatcccgttg ctgatagcca tcccgctggt
     5281 gatgggctta agcatcctgc tgctgaaaaa acaggaggga tgatgcgcca tttacgcaat
     5341 atttttaatc tgggtatcaa agagttgcgc agtctgctcg gtgataaagc gatgctgacg
     5401 ctgattgtct tctcgtttac ggtgtcggtg tattcgtcag cgaccgttac gccaggatcg
     5461 ttgaacctcg cgccgatcgc cattgccgat atggatcaat cgcagttatc gaaccggatc
     5521 gttaacagct tctatcgtcc gtggtttttg ccaccggaga tgatcaccgc cgatgagatg
     5581 gatgccggac tggacgccgg acgctatacc ttcgcgataa atattccgcc taattttcag
     5641 cgtgatgtcc tcgccggacg ccagccggat attcaggtga acgtcgatgc cacgcgcatg
     5701 agccaggcat ttaccggcaa tgggtatatc cagaatatta tcaacggtga agtgaacagc
     5761 tttgtcgcgc gctaccgtga taacagcgaa ccgttggtat cgctggaaac ccggatgcgc
     5821 tttaacccga accttgatcc cgcgtggttt ggcggggtga tggcgatcat caacaacatt
     5881 accatgctgg cgattgtatt gaccggatcg gcgctgatcc gcgagcgtga acacggcacg
     5941 gtggaacatt tactggtgat gccgataacg ccgtttgaga tcatgatggc gaagatctgg
     6001 tcgatggggc tggtggtgct ggtggtatcg ggattatcgc tggtgctgat ggtgaaaggt
     6061 gtactgggcg taccgattga aggctcgatc ccgctgttta tgctgggcgt ggcgctcagt
     6121 ctgtttgcca ccacgtcaat cggcattttt atggggacga tagcgcgttc aatgccgcaa
     6181 ctggggctgc tggtgattct ggtgctgctg ccgctgcaaa tgctttccgg tggatccacg
     6241 ccgcgcgaaa gtatgccgca gatggtgcag gacattatgc tgaccatgcc gacgacacac
     6301 tttgttagcc tcgcgcaggc catcctctac cggggtgccg gattcgaaat cgtctggccg
     6361 cagttcctga cgctgatggc aattggcggc gcgtttttca ccatcgcact gctgcgattc
     6421 aggaagacga ttgggacaat ggcgtaagga agggaaactt gacttcatac tgtgatgtca
     6481 taaagtgaag tcataaggac atgccatgag aaccaaggta caggctttac ggaagaaaca
     6541 aaaaaataca ttggatcaaa tatttaaaac tcctgttcct caagggatca agtggtctga
     6601 tatagagtca ctggttaaag cattaggcgg agaaattaag gaaggaagag gttcgcgttg
     6661 caagttcata ctaaatatga gcgttgcgtg tttccatcgg cctcatccgt cgccagatac
     6721 cgataaaggc gctgtagaaa gcgtgtgtga ctggttacta agtatagggg taaaaccatg
     6781 atcaaactga agacgccaaa ctctatggag attgccgggc aaccagctgt tattacctac
     6841 gtgccagaac tgaatgcatt tcgtggcaaa ttcctgggct tgtcaggtta ttgtgatttt
     6901 gtctctgaca gtattcaggg gctgcaaaaa gaaggagagc tttccctgcg agagtatctt
     6961 gaggattgta aagctgcggg tattgagccg tatgcgcgta cagaaaagat taagaccttt
     7021 acgttacgct atccagaatc gctgagtgaa cgccttaata atgcggctgc gcagcaacaa
     7081 gtctctgtta atacctatat tattgaaaca ctgaatgagc gtttgaatca tttataaatt
     7141 ttagcgacgt acattacgca gaatatctgc c
//
LOCUS       DAZXUK010000201         7158 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZXUK010000201 DAZXUK010000000
VERSION     DAZXUK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.201"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            166..1737
                     /gene="garD"
                     /locus_tag="ACH3L8_003208"
     CDS             166..1737
                     /gene="garD"
                     /locus_tag="ACH3L8_003208"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFT4086204.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(1789..2613)
                     /gene="agaR"
                     /locus_tag="ACH3L8_003209"
     CDS             complement(1789..2613)
                     /gene="agaR"
                     /locus_tag="ACH3L8_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFT4086205.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            2862..4142
                     /gene="kbaZ"
                     /locus_tag="ACH3L8_003210"
     CDS             2862..4142
                     /gene="kbaZ"
                     /locus_tag="ACH3L8_003210"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFT4086206.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            4165..4638
                     /gene="agaV"
                     /locus_tag="ACH3L8_003211"
     CDS             4165..4638
                     /gene="agaV"
                     /locus_tag="ACH3L8_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFT4086207.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            4649..5428
                     /gene="agaW"
                     /locus_tag="ACH3L8_003212"
     CDS             4649..5428
                     /gene="agaW"
                     /locus_tag="ACH3L8_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFT4086208.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            5418..6227
                     /gene="agaE"
                     /locus_tag="ACH3L8_003213"
     CDS             5418..6227
                     /gene="agaE"
                     /locus_tag="ACH3L8_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFT4086209.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            6314..6748
                     /gene="agaF"
                     /locus_tag="ACH3L8_003214"
     CDS             6314..6748
                     /gene="agaF"
                     /locus_tag="ACH3L8_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFT4086210.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            6745..>7158
                     /locus_tag="ACH3L8_003215"
     CDS             6745..>7158
                     /locus_tag="ACH3L8_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFT4086211.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         1692 a         1861 c         1906 g         1699 t
ORIGIN      
        1 tgcgctttac cttgccgggc ggcactgcaa tccctaaaat gattgacatt gatcacattt
       61 ctgcgtttaa actcctgaca ttcttatttc acccaatgaa atcatttatt tttaaatgag
      121 accaggtcct cattttaata acccctggct ggagaatatt gcacaatggc caacatcgaa
      181 atcagacaag aaacgccaac tgcgttttat ataaaagttc acgacacaga taatgtggca
      241 attattgtta atgataatgg cctgaaagca ggaacgcgtt ttccggatgg gctggaatta
      301 attgaacata ttccccaggg gcataaagtc gcattgctgg acattccggc taatggtgaa
      361 attattcgtt atggcgaagt gattggttac gccgtgcgtg caatcccacg cggaagctgg
      421 atcgacgaat caatggttgt actaccggaa gcgccgccgt tacacacgct gccactggca
      481 accaaagtcc cggaaccctt accgccgctg gaaggataca cctttgaggg ctatcgcaat
      541 gccgatggca gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt ccactgtgtg
      601 gcaggtgtgg tggactatgt agtaaaaatc attgaacgcg atctgctacc gaaatacccg
      661 aacgtcgatg gcgtggtggg gctgaatcat ttgtacggtt gtggcgtggc gattaacgca
      721 ccggcagcag ttgtacctat tcgtaccatt cacaatattt cgctgaaccc taactttggc
      781 ggcgaagtaa tggtgattgg cctgggttgt gaaaagttgc agcctgagcg cctgctgact
      841 ggaacggatg atgtgcaagc tattccagta gaaagcgcca gcattgtcag tttgcaggat
      901 gaaaagcatg tcggttttca gtccatggtc gaggatattt tgcaggtcgc cgaacgccat
      961 ctacaaaaac tgaatcaacg tcagcgagaa acctgtccgg cttcagaact ggttgtcggc
     1021 atgcattgcg gtggcagcga tgcgttttct ggcgtaacgg caaacccggc ggttggctat
     1081 gcgtctgatc tactggtgcg ctgcggcgca acggtgatgt tttcagaagt cacggaagtg
     1141 cgtgacgcga tccatctgct gacaccacgc gcagtgaacg aagaggtcgg caaacggctg
     1201 ctggaggaga tggagtggta cgataactat ctcaatatgg gaaaaaccga ccgcagcgcc
     1261 aacccttcgc cgggcaacaa gaaaggcggt ctggcaaacg tggtagagaa agcgctcggc
     1321 tccattgcta aatcgggcaa aagtgcaatt gttgaagtgc tgtcgcccgg tcaacgcccg
     1381 actaaacgcg gattaattta cgccgcgacg ccagccagcg attttgtctg tggcacgcaa
     1441 caggtggctt cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac gccgtacggc
     1501 ctgatggcgg tacccgtcat taaaatggca ccccgcaccg agctggcgaa ccgctggttt
     1561 gatttaatgg atattaatgc gggcaccatc gctaccggcg aagaaactat tgaagaggag
     1621 ggctggaagt tgttccactt tattctcgac gtcgccagcg ggaagaagaa aaccttctcg
     1681 gatcaatggg gactgcataa ccagctggcg gtgtttaacc cagcaccggt gacctgataa
     1741 ttcaattcgt attatgcccg atagcacacg tttatcgggc atattgagtc acagcgaaga
     1801 cgcgtgatcc ccgaccagaa tcacttcaac cccagccttt cgcagtcctt ccagactatc
     1861 cgcaggaatg ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg
     1921 taaactggaa cgattgaatt tactggaatc ggtgaccacg atgatccgtt ccgcaacttc
     1981 gcacatccga cggcttaaac gggcttcatc ttcattatgt gtgctgacgc cgcgctccag
     2041 atcgatcgca tctacaccaa gaaacagcat atcgaagtgg taattttgca gcgattgctc
     2101 agcctgatcg ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg tcatcagcag
     2161 ctcaacgcct tccgcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat
     2221 tacgtcagtg tacttgcgca tcagacgagc aatctcaaaa gtggtggtcc cggaatcgag
     2281 gatcacccga tgacctggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat
     2341 cgcggtgttc agtgcgcttt tatcttccac tgatggctcg actgacggcg tcgtgctatc
     2401 gcagatcaac gcgccaccat aggcacgcac agcgatcccc tgcttttcca gaaacgccag
     2461 atcattgcgg atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac
     2521 actcccttgc tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg tgcctgtcac
     2581 tcgcttctca cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt
     2641 tcatttcgtt ttgcctatta acgcctttct attaagcaaa tgcaagccca ccttgcccat
     2701 tggcgcaagc tactctcgtt tcagtgactt tcattacgtt tcttttgtga atcagatcag
     2761 aaaactatta tctttcgttt tatttttatc tcaccatgac gcagtatcaa ttgaaacaaa
     2821 acgaaagatc aatatcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa
     2881 tggtgagaca gcacaaagcg ggcaaaacaa atgcaattta tgccgtttgt tccgcacatc
     2941 cgctggtgct ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg
     3001 aagcaacctc caatcaggta gaccagttcg gcggttatac cggaatgacg cccgccgatt
     3061 ttcgcggctt tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgctgattc
     3121 tgggtggtga ccatctgggg ccaaaccgct ggcaaaacct gccagccgct caggcaatgg
     3181 ccaatgccga tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa atccaccttg
     3241 attgcagcat gtcctgtcag gacgatccaa ttcccttaac tgatgacatc gtggctgaac
     3301 gcgccgcccg tctggcgaaa gtggcggaag aaacctgtcg cgaacacttt ggcgaagccg
     3361 atctggagta tgtcattggt accgaagtgc cggtacctgg cggcgcgcat gaaaccttaa
     3421 gcgagctggc ggtcaccacg ccggatgccg cccgcgccac gctggaagcc catcgtcacg
     3481 cctttgaaaa gcaaggtttg aatgccatct ggccacgcat tattgccctg gtggttcaac
     3541 ccggcgtcga attcgatcac accaacgtta ttgattatca gcccgccaaa gcgagcgcct
     3601 taagccagat ggtcgaaaac tacgaaacgc tgattttcga agcgcactct accgattatc
     3661 aaacgccgca atctctgcgc cagctggtga ttgaccactt tgccattctg aaagttggcc
     3721 cggcgctgac cttcgccctg cgtgaagccc ttttttctct ggcggcgatt gaagaagaac
     3781 tggtaccagc gaaagcctgt tccggtctgc gtcaggtgct ggaaaacgtg atgctcgacc
     3841 gcccggaata ctggcaaagc cactaccacg gtgacggcaa cgcgcgtcgt ctggcgcgtg
     3901 gttacagcta ctcagatcgc gtgcgctatt actggccgga cagccagatt gatgacgctt
     3961 tcgctcatct ggttcgtaat ctggcggatt caccaattcc gctaccgctc atcagccagt
     4021 atctgccgct gcagtacgtg aaagttcgct ccggcgagct gcaaccgacg ccacgggaac
     4081 tcattataaa ccatattcag gacatcctgg cgcagtacca cacagcctgt gaaggccaat
     4141 aagcaaaaca aagaggaaca tgctatgcca aatattgttt taagccggat tgatgaacgc
     4201 ttgattcacg gtcaggtcgg cgttcagtgg gtcggatttg cgggggcaaa tctggtgctg
     4261 gtagccaacg atgaggttgc cgaagatccg gtacaacaaa acctgatgga aatggtactg
     4321 gcagaaggga tcgccgtacg tttctggacg ctgcaaaaag ttatcgacaa cattcatcgc
     4381 gccgccgatc gacagaaaat cctgctggtt tgtaaaacac ccgccgattt cctgacgctg
     4441 gtgaaaggtg gcgttccggt gaatcgcatt aacgttggca atatgcacta cgccaatggc
     4501 aaacaacaaa tcgccaaaac ggtttctgtg gatgcgggcg atatcgcagc atttaacgac
     4561 ctgaaagccg ctggggtgga atgcttcgtt cagggcgtcc cgacagagcc tgctgtggac
     4621 ctctttaaat tactttgagg gattcatcat ggaaatcagc ctgttgcagg catttgcgtt
     4681 gggcattatc gcctttatcg ctggcctaga tatgtttaac ggcttaaccc atatgcaccg
     4741 cccggtggtc ctcggcccgt tggtcgggct ggtacttggc gatctgcata ccggaatttt
     4801 aaccggcgat acgctggaac tagtgtggat ggggctggcc ccgctggcgg gcgcacagcc
     4861 gcctaacgtg attatcggta ctatcgtcgg cacggcgttt gccattacta ctggcgtgaa
     4921 acccgatgtc gcggtcggtg tcgccgtacc tttcgctgtc gcagtacaga tggggattac
     4981 cttcctgttc tcggtgatgt ctggcgtgat gtctcgctgc gaccggatgg cggagaacgc
     5041 cgatactcgc ggcattgaac gcgtgaacta tctggcactg ctggcactcg gcactttcta
     5101 ttttctctgc gctttcctgc ctatctactt cggcgcggaa catgcaaaaa ccatcattga
     5161 tgtcctgccg caacgattaa tcgacggcct cggcgtcgcg ggcggcatca tgccagcaat
     5221 cggttttgcc gtgctgctga aaatcatgat gaaaaacgtc tacataccct acttcatcct
     5281 gggctttgtt gccgcaacct ggctcaagtt accggtgctg gctattgctg ccgccgcgct
     5341 ggcgatggcg ctgatcgatc tgctgcgtaa atctcctgaa ccgactcaac ctgcggcaca
     5401 gaaagaggaa tttgaagatg gcatctaatc aaaccaccct gccgaacgtc agcgaaaatg
     5461 aagaaacact gctgactggc gtcaatgaaa acgtgtatga agatcggagc attgccgcgg
     5521 agctgacgaa aaaagatatc aaccgcgtcg cctggcgttc catgctgtta caagcgtctt
     5581 ttaactacga acgtatgcag gcttctggct ggttgtacgg tctgctgcct gctctgaaaa
     5641 agatccacac taataaacgc gacctggcgc gcgccatgaa ggggcatatg ggtttcttca
     5701 atacccatcc gtttctggtg acgtttgtta tcggcattat ccttgcgatg gagcgttcta
     5761 agcaggacgt taacagtatt cagagcacca aaattgccgt cggtgcgccg cttggcggga
     5821 ttggcgatgc gatgttctgg ctaacgctac tgccgatttg tggcgggata ggcgcaagcc
     5881 tcgcgctaca aggctctatt cttggcgcag tcgtctttat tgtgctgttc aacgtggtac
     5941 acttggggct gcgttttggt ctggcgcatt atgcttaccg catgggcgtg gcggcgattc
     6001 cccttattaa agcgaatacc aaaaaagtcg gccatgctgc gtcaatcgtt ggaatgacgg
     6061 taatcggcgc actggtggca acctatgttc gtttaagcac cacgctggaa atcacggcgg
     6121 gcgacgcagt agttaagtta caggctgatg ttatcgacaa actgatgcct gccttcttac
     6181 cgctggtcta caccctgacc atgttctggc tggtacgccg cggctagagt ccgctgcgcc
     6241 tgattgcggt taccgtggtt ctagggatcg tcggtaaatt ctgccatttc ctttaaatac
     6301 aatgaggtat tagatgttaa gtattatttt gacagggcat ggcgggtttg ccagtggcat
     6361 ggaaaaagca atgaagcaaa tcctcggcga tcaatcgcag tttattgcca tcgattttcc
     6421 cgaaacctca agcgccgcgc tgcttacatc gcagcttgaa gaggccatcg cccaactgga
     6481 ttgtgaagac ggcattgttt ttctgacgga tttgctgggc ggcacgccgt ttcgcgtagc
     6541 ttcaacgctg gcgatgcaaa aaccgggctg tgaagtgatt accggcacca atttgcaact
     6601 gctactggag atggtgctgg agcgcgaagg gttaagcggt gaagaattcc gtgtacaggc
     6661 gctggagtgt ggacaccgag ggctgaccag tctggtggac gagttaggcc gctgccatga
     6721 agaatgtccg gtcgaggaag gaatatgaca cacgttctgc gcgccagaag gctgctgact
     6781 gaagagggat ggttcgatga ccatcagttg cgtgttgctg acggtgtcat cgcagcaatc
     6841 gaaccgattc cagtgggcgt gactgaacgc gatgcggaac ggctctgccc cgcttacatc
     6901 gacacccatg tacacggtgg tgcgggcgtt gatgtaatgg atgacgcgcc ggatgtactc
     6961 gacaagctgg caatgcacaa ggcacgcgaa ggtgtcggca gttggttacc gaccaccgta
     7021 accgcgccgc ttaataccat tcatgcggcg ctgaaacgta ttgctcaacg ttgccaacgc
     7081 ggcggacctg gtgcgcaagt gctggggagt tatctcgaag gaccgtactt cacgccgcag
     7141 aataaaggcg cgcatccg
//
LOCUS       DAZXUK010000202         7052 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZXUK010000202 DAZXUK010000000
VERSION     DAZXUK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.202"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..128)
                     /locus_tag="ACH3L8_003216"
     CDS             complement(<1..128)
                     /locus_tag="ACH3L8_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFT4086212.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAV"
     gene            complement(392..1126)
                     /gene="cysH"
                     /locus_tag="ACH3L8_003217"
     CDS             complement(392..1126)
                     /gene="cysH"
                     /locus_tag="ACH3L8_003217"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFT4086213.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1200..2912)
                     /gene="cysI"
                     /locus_tag="ACH3L8_003218"
     CDS             complement(1200..2912)
                     /gene="cysI"
                     /locus_tag="ACH3L8_003218"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFT4086214.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2912..4711)
                     /gene="cysJ"
                     /locus_tag="ACH3L8_003219"
     CDS             complement(2912..4711)
                     /gene="cysJ"
                     /locus_tag="ACH3L8_003219"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFT4086215.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5030..5392
                     /gene="queD"
                     /locus_tag="ACH3L8_003220"
     CDS             5030..5392
                     /gene="queD"
                     /locus_tag="ACH3L8_003220"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFT4086216.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5470..6741
                     /locus_tag="ACH3L8_003221"
     CDS             5470..6741
                     /locus_tag="ACH3L8_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFT4086217.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            6732..6992
                     /locus_tag="ACH3L8_003222"
     CDS             6732..6992
                     /locus_tag="ACH3L8_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFT4086218.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
BASE COUNT         1538 a         1934 c         1833 g         1747 t
ORIGIN      
        1 actgccttaa actccatacc acgttctctg atactcaact cacataacga gtcacctacc
       61 atcaacgtga atcccagtac tgtacaacac agtacgatga ttgccacaag ggcatatttt
      121 gtcagcatgt cttgcctccc tgcaaagaag agactaacat ccgaattgct acgttcgaac
      181 gcgagcctca ggttgataga aatatcgcct ggggcttttg tccatctgga acctcgcgaa
      241 tgcttaacgc cagacagcct caagcaccca acgccattct atacctgtta attttccctg
      301 cgatgttcac ctttaaatgc cggaatgctg ctcgcaaaat aaaaacgccc ccatcatttc
      361 tgacagaggc gtttaattta tccggcaata tttacccttc gtgtaatcca cattcccttt
      421 ttaagccaaa gaaacgcgtt tcttcttccg ccatgccggg ttcccattta cgggttgtat
      481 gggtatcgcc caccgataaa tatccttcgt cccataatgg gtgatatttc aggccatgtt
      541 tttgcaggta ctgataaata gttcggttat cccagtcgat aatcggcagc actttaaata
      601 cgccacgctg aattgccagc accggtaaat tggcacggct gccggattgt tcacgccgca
      661 ggccagcaaa ccacgtttgc gcattcagtt ctttcagcgc ccggttcatc ggttcgactt
      721 tgttgatgtc attgtacttt tcaatgcctt caacgccctg ttcccacagt ttgccgtagc
      781 gtgcttcctg ccaggcggcg ctttcggtag cgcggtacac tttcaggttg agcttgagtt
      841 tgtccgttaa ctcgtcaata aagcggtagg tttccgggaa cagatagccg gtgtcggtga
      901 ggatcaccgg aatatccggg tgaatctgat tcaccagatg caggctcacc gccgcctgaa
      961 tgccaaagct ggaagaaagt acatattcac cgggcagatt atccagcgcc caggcaacgc
     1021 ggccttcagc gtccagtttt tccagttcgg cgttagtttc tgccagcgcc agaatgcgat
     1081 ctaccttcgg cagttcgttc agggcgttta gatcgagttt ggacatagat tcctcactgt
     1141 ttgccttgcc tgatgcgacg ctcacgcgtc ttatcaggcc tacaagaccg ggctgatggt
     1201 taatcccaca aatcgcgcgc cggatcgagc accgggcgaa tgatgcccgc acgcaccgta
     1261 aagtcgccga agccttcacc cgcttcgcgc tctttcgccc agcgccctat cagttcatca
     1321 agcgacgcca ggatttccgg ctcggtgatg ttttctttat acatccgtgg gatacgtgtc
     1381 ccaatgcggt tgccgccaag atgcaggttg tagcgacccg gtgctttacc caccaggccc
     1441 acttccgcca gcatcgcgcg accacaaccg ttcgggcagc ctgttacacg catcacgata
     1501 tgctcatcgc tgacaccatg tttcaccatt aaattatcga tgttgtcgat aaaagacggc
     1561 aggaaacgct ccgcttccgc catcgccagc gggcaagtcg ggaatgacac gcaggccatc
     1621 gagttttcac gctgcggcgt gacggcattc attaacccgc tctctttggc aattttctcg
     1681 atcttcgctt tctcgctttc cggcacaccg gcgatgatca gattctggtt cgccgtaatg
     1741 cggaaatcac ctttgtggat cttcgcgatc tccagcaggc cagttttcag cggacgcccc
     1801 ggataatcaa ggatgcgacc attttcgata aacagcgtca ggtgccagtt atcatcaatg
     1861 cccttaaccc agccaatacg atcgccgcgt cctgtaaatt catacgggcg gatcggttca
     1921 aatttgatcc ccgcgcgacg ctccacttcc gctttaaacg tctcaacccc cacgcgctcc
     1981 agtgtgtatt tggttttggc atttttacga tcggttcggt taccccagtc acgctgagtt
     2041 gtcacgacgg cttccgccac cgctagcgta tgctctagcg gcagatagcc aaactcactc
     2101 gccgtgcggg cgtaggtttt cttgttaccg tgttcgatag aaagcccacc gcccaccagc
     2161 aggttgaagc ccaccagctt gccgttttcc gcgatcgcca cgaagttcat gtcgttggcg
     2221 tgcagatcaa tatcgttctg cggcgggatc actaccgtgg ttttgaactt acgcggcagg
     2281 taagtctgac caaggatcgg ttcttcatcg gtggtggcga ctttttcctg atcgagccag
     2341 atctccgcat acgcgcgggt acgcggcagc agatgttcag aaatcttctt cgcccactcg
     2401 tacgcttctg cgtgcagctg cgactcgtaa gggttcgagg tgcagagtac gttacggttc
     2461 atgtcgttag cagtcgccag cgcatcaaga ccgaccgagt gcagcatctg gtgcaccggt
     2521 ttgacgttct ttttcagaat gccgtggaac tgaaacgtct ggcggttggt caggcgaatg
     2581 ctgccataga tggtgttttc accggcaaat ttatcgatcg cctgccactg tttggtggtg
     2641 ataactccac ccggcagacg gcagcgcaac agcatcgcgt ggcgcggctc cagcttctgt
     2701 tcagcacgtt cggcgcggat gtcgcggtca tcctgctgat acatgccgtg gaagcggatc
     2761 agcaggaagt tatcgccctt aaagccgccg gtcagaccgt cgtttaaatc ttccgcaatg
     2821 gtgccgcgca ggtagttgct ttcaagcttc atgcgctcgg cgtctgtcag ttttccttcg
     2881 accactaaag gccctggatg tttttcgctc attagtagac atctcgctga taacggcgct
     2941 ctacgcgcag ctcacttaaa aattcatccg ccgcttcggt gtccatgcca ccaaattcgg
     3001 caatcacttc cagaagtgcc tgctcaacgt ctttcgccat gcgattagcg tcgccgcaga
     3061 cataaatgtg ggcaccgtca ttgatccagc gccacagctc cgcgccctgt tcgcgcagtt
     3121 tatcttgtac gtaaactttt tctttttggt cgcgcgacca ggcgagatcg atccgcgtca
     3181 gcacaccctc tttgacgtag cgctgccact ccacctgata caggaagtct tccgtaaaat
     3241 gcggattgcc aaagaacagc cagtttttac ctggcgcttc gtcagcggcg cgttgctgca
     3301 taaaggcgcg gaacggcgca ataccggtgc ctgggccaat cataatcacc ggggtttcca
     3361 ggttagcggg caggcggaag ttatcgttat gttcgataaa tacgcggact tcgccctctt
     3421 cttccacgcg gtcagcgagg aagctggagg caccaccggc acgggcgcgg ccttccacgt
     3481 cgtaacgcac cacaccaacg gtgacgtgta cttcgttctc gacttccgcc tgcgaggagg
     3541 caatggaata gagacgcggg gtcagcgggc gcagcagatt aattagcgct tcggcatcaa
     3601 gctgtgccgg ggagaaacgc accatgtcaa caatcagcgt cgtcgcggcg taatgctgta
     3661 acttcgcttt atcgcccacc agcggcagca gtgtttcact gcgggtaagc gtggcgtaat
     3721 tctcaacaat gttggcggtg ttgacggtca gttcgaagtg ccactgtagc gcttcgttca
     3781 gaggcaacgt tttgccctcg acagtgacag gttcatcgcc tgtcagccac agcagttcga
     3841 caagttcttt caccagtgcc ggatcgttct gataccagac gcccagcgcg tcacccggct
     3901 ggtaacgcag gcccgagtcg cctaagtcaa tttcgatatg gcgaacgtct ttttcagagt
     3961 tacgcccagt aattttctgg ttaacagaga ggctagccac cagcggcgcg tctttgctgt
     4021 acgggctggt gtggatttca tttaccgcgc cagtagcgac ggattgcgaa ggtgccgcga
     4081 caggcgcacg cgatttaagc gcatcaacca cgcgggcgcg ccactcgctg gcagcagcct
     4141 ggtattcaac atcagaatcg acacggtcga gcaggcgttc accccccagt tccgccagct
     4201 tgctgtcaaa atctttcccg gactggcaga aaaattcata agagctatcg ccgaggctaa
     4261 acacggcaaa cgcggtgttt tccagctttg gcgctttttt ggagaacagg aacttatgca
     4321 gcgcgacggc ttcttccggc ggttcccctt ccccttgcgt tgacgtcact acgatgagca
     4381 gtttttcgct ggcgatttgt ttgaatttat agtcgcccgc gttcaccagc ttaacgttca
     4441 gttttgctgc taataaatca tcgcgtaatg cttcagcaac ccggcgcgca ttgccggttt
     4501 gcgaagcaga gataatcgtt atacccggca tttctgcggc gggcgctggc gtcactgcaa
     4561 gcgcaacagg ctgctggttg agcacgcccc agaaatagcc agaaacccag gcaagctggg
     4621 taggcgttaa atcggtcgtg gccgcctgaa ggcgtgccag ttgctccggg ttcaacggaa
     4681 gcaacgcgga aggtgggacc tgtgtcgtca tgcgtcgtta tgttccagta agcaaagctg
     4741 tttctgcgcc ctgtcagcgc ccataaaaca gaagagaagg taaggttaac ggggtgaacg
     4801 gtgtggatta aagacgggat agcgataact aataaccaaa tcgactaacc tgttttagca
     4861 atagtcttta acaacaaaat agattaacca acctaatgaa aaacaaatga atttagccaa
     4921 tcattaagat aaatcagcga ttttgcgcaa caaagtcgtt ttagataatg cgaaaaaaca
     4981 gcctttccgg tactctacgg cggttttatc gtcctgtaga gaaattatga tgtccaccac
     5041 gttatttaaa gatttcacct tcgaagccgc tcaccgctta ccacatgtcc cggaagggca
     5101 taaatgtggt cgcctgcacg ggcattcctt tatggtgcga ctggaaatta ccggggaagt
     5161 cgatccgcat acgggctgga ttatcgattt cgctgaactg aaagcggcgt ttaagccaac
     5221 ttatgagcgc ctcgatcacc attatctcaa tgatattcca ggtctggaaa atccaaccag
     5281 cgaagtttta gcaaaatgga tttgggatca ggttaaaccc gttgtgccgc tgttaagtgc
     5341 ggtgatggta aaagaaacct gcaccgcagg gtgtatctat cgcggcgaat gataagagtg
     5401 tgtcggcggt caatttccct ttagtaacgc tatgttaggg tgttgtgttc tggatatctg
     5461 gggcatgaca tggaagacga ctgcgacatt attattattg gtgccggtat tgcaggcacc
     5521 gcttgcgcgt tacgctgcgc gcgagcggat ttatccgttt tgttactcga acgcgctgaa
     5581 atccccggca gcaaaaatct ttccggcagg cggttatata cccatgcact cgcggaactc
     5641 ctcccgcaat ttcatctgac cgcgcctctt gaacgacgca tcactcacga aagcctttcc
     5701 ctgttaacgc cggatggcgc aacgacgttt tccagcttac agcccggcgg tgaatcctgg
     5761 aatgtattac gtgcacggtt cgatccgtgg ctggttgccg aagccgaaaa agaaggtgtc
     5821 gaatgcatcc ccggagcgac ggtggatgca ctgtatgaag aaaacggcag agtctgtggc
     5881 gttatttgtg gtgacgatat tctccgcgcc cgttatgtgg tgctggcaga aggtgccaac
     5941 agcgtcctgg ctgaacgtca cgggttagtg actcgtcctg ctggcgaagc gatggcgttg
     6001 gggatcaaag aagtgctgtc gctggaaaca tccgctattg aagaacgttt tcatctggag
     6061 aataacgaag gcgcagcgtt gctgttcagc ggcggaatct gtgatgactt acccggcggc
     6121 gcatttcttt atactaatca acaaacgctc tcgttaggga ttgtttgccc gctctcttcc
     6181 cttacgcaaa gtcgtgttcc ggcaagcgag ctgctgactc gctttaaagc gcatccggca
     6241 gtgcgcacgc ttatcaaaaa cacggaatca ctggagtatg gtgcgcatct ggtgccagaa
     6301 ggtggcttgc acagtatgcc ggtgcaatac gccggtaacg gctggctgct ggtgggcgat
     6361 gcgttgcgca gttgcgtcaa taccggaatt tccgtgcgcg gcatggatat ggcgctgact
     6421 ggcgcacagg cggcggcaca aacgctaata agcgcctgcc agcaccgcga gccgcaaaat
     6481 ctgtttgcgc tttatcatca caacgtagag cgcagcctgc tatgggatgt tctacaacgt
     6541 tatcagcatg ttccggcgct tttgcaacgc cctggcaggt atcgggcgtg gcctgcgtta
     6601 atgcaggata tttcccgcga tttatgggat cagggtgata aacctgttcc accgctgcgc
     6661 cagttattct ggcatcattt acgtcgtcat ggcctgtggc atctggcggg cgatgttatc
     6721 aggagtctgc gatgtctgta gcccgtaatc tctggcgcgt tgctgatgcg ccgcacattg
     6781 ttccggctga ctccgttgag cgtcagatgg tacaacggtt gattaacgcc tgtccggtag
     6841 gtcttttttc tctcacacag gaaggtgact tacgtattga ctatcgcggc tgcctggagt
     6901 gtggcacctg ccgtttgctg tgcgacgaat ccacactaca acagtggcgc tatccgcctt
     6961 ccggattcgg catcacctac cgctttggat aaataataag gataatttat gcccctctta
     7021 cacctgctcc gccagaatcc ggtgattgct gc
//
LOCUS       DAZXUK010000203         7020 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZXUK010000203 DAZXUK010000000
VERSION     DAZXUK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7020
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.203"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..146)
                     /locus_tag="ACH3L8_003223"
     CDS             complement(<1..146)
                     /locus_tag="ACH3L8_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086219.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSL"
     gene            224..1204
                     /gene="ddlA"
                     /locus_tag="ACH3L8_003224"
     CDS             224..1204
                     /gene="ddlA"
                     /locus_tag="ACH3L8_003224"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFT4086220.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINTLPGFTNISMYPKLWQASGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1228..1440)
                     /locus_tag="ACH3L8_003225"
     CDS             complement(1228..1440)
                     /locus_tag="ACH3L8_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFT4086221.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1700..2008
                     /locus_tag="ACH3L8_003226"
     CDS             1700..2008
                     /locus_tag="ACH3L8_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFT4086222.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2067..3161)
                     /gene="yaiW"
                     /locus_tag="ACH3L8_003227"
     CDS             complement(2067..3161)
                     /gene="yaiW"
                     /locus_tag="ACH3L8_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFT4086223.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3174..4394)
                     /gene="sbmA"
                     /locus_tag="ACH3L8_003228"
     CDS             complement(3174..4394)
                     /gene="sbmA"
                     /locus_tag="ACH3L8_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFT4086224.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4746..5903
                     /gene="ampH"
                     /locus_tag="ACH3L8_003229"
     CDS             4746..5903
                     /gene="ampH"
                     /locus_tag="ACH3L8_003229"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFT4086225.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5904..6407)
                     /locus_tag="ACH3L8_003230"
     CDS             complement(5904..6407)
                     /locus_tag="ACH3L8_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4086226.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(6495..>7020)
                     /locus_tag="ACH3L8_003231"
     CDS             complement(6495..>7020)
                     /locus_tag="ACH3L8_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFT4086227.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         1780 a         1801 c         1690 g         1749 t
ORIGIN      
        1 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
       61 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
      121 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
      181 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
      241 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
      301 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
      361 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
      421 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
      481 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
      541 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
      601 gtttgtaggt tctgatgttc tggcttcagc agcctgtatg gataaagatg tcaccaaacg
      661 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
      721 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
      781 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
      841 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
      901 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
      961 gatcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     1021 gaaagtggta gttccggcag ccattgcacc agagatcaac acactgcctg gcttcaccaa
     1081 catcagtatg tatccgaagc tgtggcaagc cagcggtctg ggttacaccg atctgatcac
     1141 acgtttgatt gagctggcgc tggagcgtca cgctgcggat aacgcattga aaaccacaat
     1201 gtaatatttt aacgcccgat aatattctta ttgggcgttt tcagtttctt catcgcgacg
     1261 acgaataatc aaccccgcca gccagaaact aatcacccac gtcaccagac cgacggcata
     1321 ggtctgccaa ccttttgctt caaatcccag tacccccacc acgccattaa gaatgaatat
     1381 aaggccaatg gcgaacgcat agtagtgcca gtcacggcgg atttttacag gcagattcat
     1441 ggcagctccg ttaacgaaaa acaacatcct tgcatattct gtaacaaccg tctgtggtgt
     1501 agacaacatt ttcgtaatta ttaataaatt taacttaagg ttgccatttt gtgactcaca
     1561 acgaagattc acgcttcggg aatattccca acatcaattt accatgattc tgatattgac
     1621 aatctaaatg acctgtcaga cttgccataa gccgctggta tcacaccaga agagaagcag
     1681 atgacttccg gaggttttta tggctgattt caccctgtca aaatcgctgt ttagcggaaa
     1741 atatcgcaat gcctcttcaa cgcctggcaa cattgcctat gcgttgtttg tgctgttttg
     1801 cttttgggct ggggcgcaat tgctgaacct gttagtgcat gcgcccggcg tctatgagcg
     1861 tttaatgcag gtccaggaaa caggtcgccc acgggtggaa attggtttag gtgtcggcac
     1921 cattttcggg ctgatcccgt ttttagcagg ctgcctcatt tttgcagtgg tggcgctatg
     1981 gctgcactgg cgacatcgcc gccagtaaaa tttacgttgc cagatgcggc ctgaaacgcc
     2041 ttatctggcc tgacattcct gtttgatcac tgtttcatac aacgcgcccg ccgttcgtct
     2101 acgcgcttcg caaaccaggc cgtagtcagg ttgcgggtga ttttcgggct ttccagttga
     2161 atgccaggca acatttcgcg gggtagtgat ttacccgttt tcgcttcggc aagttgataa
     2221 actttcttgt acagcgccgt ctcctcaaaa gagaagctgt cacctttctc taactgacgg
     2281 cgaatttcgc tgtcgctcat ccccagtttt cccgccagtt tgcgcgttgc cagttcggtt
     2341 ttcccgggtt ctttactgtc gtaacgaatt aaatcaccgt ccagtgccag cttcacgccg
     2401 ctggccttac tgactgcgtt ctgaaacgcg gcattacggc tggcgtacca gccagcgtta
     2461 aaatcagcaa agcggtatat cggtgcgcta tagctggcgg gataattcag taaatggtaa
     2521 gtaccaaacc acaacccgcc gcggcgactg aagacttcct gacgaactgt accgtccatt
     2581 ttccacggat accctttggt atgctgttcg gcaaaagcaa tgctgacctg catcggccca
     2641 ccggtgcgca ccggattaag cgaaccaaac agcgtctgcc ccattggtac catgctgatc
     2701 agatcgtcaa aaatcgcgct caattgcttt tctgtacgca ccgaatctaa ccgttcgcta
     2761 taacttttgc cgtttggcga tttgattttt agcgcggtat gaaccagaaa agcaggaatg
     2821 tgcatccgtt cagcacgacg gtcaatctct tgccaggcga tcttgcttaa gcccggaacg
     2881 gccggatctg cctgataatt tgactcctgc tgcgccaccg ccagcaccga gtagacgttt
     2941 tccagcgtcg gtgccagccc ctgactttca aaggtggtgg cgagatcttt cgcccaggca
     3001 tcgcggtctt tcacgctcgc aggcatcttc tgccgcacga cgctcgctac atctatcgct
     3061 ttttcgccct ttttcaacgg ttgcggagcc tgactactac accccgctaa aaccagaacc
     3121 gccaacaatg acagcgaaga tagatgattg acgcgcgaca ttacttctcc tttttagctc
     3181 aaggtatggg ttacttcctg aattttgtca ccatccagct catgttcaaa gctgcgcaga
     3241 cgtttgtaga tagacatcaa ctcaaccagt gtggtccatg agttaatcag gtactggaaa
     3301 gcaccgcgaa cctgaccaaa aacgttggta atctgcgtca tcaggccgag cgtaatcgta
     3361 ccggcaacaa ttgacggaaa cagcaagaac aaaccgaaaa cgttatcgac ctgcaaatag
     3421 agaatgcggg cgatgttgaa atacatatag tgaaaataga ggcgaaaata gtttttccgt
     3481 acggcgctaa acagctcgcg taccgtaggc ggcgtcgcgc gcgtggcatc gtcttcacca
     3541 taaaccagct ctttacggta ggcggcctct actcgctggt ttttaaactc cagccccggc
     3601 agtttgatcc ctaccactgc cagcaatccg gtccccatca gcgaccagac gatagcggca
     3661 atcaccagac catacggaat gtgcccgata atcggcagct ccggcacatg cgcggagagc
     3721 gttaccagca ccggcaggaa ggcgatcaac gtcatgatgg cgttgataaa actgacgccc
     3781 atattctcca gcgttgaagc aaaacgcatg gtgtcttcct gcacacgctg tgcggcacct
     3841 tcgatatgac gcagttgttg ccagttcgcc atgtaatatt cgttcatcgc cgtacgccag
     3901 cggaacacgt agtgactgac aaagaagttg ttcagtacac tgatcaccac cgcgatcagc
     3961 gcaatcccca gaaagacgcc cacttcgcgg taaaattgtt cgatagtgac tttatgcggc
     4021 gaacttagcg cggtttgaat cagatcatag aacggcgcat accaggcgtt gacggcgacc
     4081 ccgacttcca ccaaaaacca agtgacgaag ataatcagcg cagtaccgag tattgaccag
     4141 tattgccaac ggtgcgggct gtagataaac cagaaaaatg caaaaagtcc tacgcaaaca
     4201 atgtagtaag cgtaaaaaat caggaaatcc aacgaccaga aacgcgcggc gctaatcggg
     4261 atctgcccgg aagcgccggt gatacgtgcc acccagtcac ccccaccggc ttgccagaag
     4321 ataacggcaa tcaatgccca aacaaaggcc gagagaaaaa acgttcccgg ctttgggaaa
     4381 aaagacttaa acatatgcac tcctgctaac ttcttatcgt tttaacaact gctgtgtatt
     4441 aaccgcatga ccgctacaaa gcgtcagcca aatgaaaccg cccattgtga cccatagcaa
     4501 ggcgcttagt tcgccagcct gaccataaga attgtttcga cggatttcac aaaccaaaga
     4561 ttgtttacat aagcggatta acacgcgcag gatcacaaat gcaggcattg gtgagcagga
     4621 aaaagatcgt gtttacgcgc cgttacgttt ttaacactat tattgcacta attcgccctt
     4681 tgcaatccat caatgagtag tataaatacg ctcagttacc ttcattcaat ctatggacac
     4741 caccgttgaa acgtagtctg cttttttctg ccgtgctgtg tgcggcgtca ttgacctccg
     4801 tccatgcggc gcagccgatc actgaaccgg agtttgcctc tgatattgtc gatcgttatg
     4861 ccgatcatat tttttacggc agcggtgcca cggggatggc gctggttgtt atcgacggta
     4921 accagcgcgt ctttcgtagt tatggtgaaa cgcgacctgg taataacgtt cgcccgcagc
     4981 tggattcggt cgtgcgtatt gcttccctca ccaaactaat gaccagtgaa atgctggtga
     5041 aattgctcga ccaggggacc gtaaagttaa acgatccgct aagtaaatat gctccgccag
     5101 gcgcacgcgt gccgacctac aatgggacgc ccattaccct agtgaatctg gcaacccata
     5161 ccagcgccct gccccgtgaa cagcccggtg gcgcggcaca tcgtccggtg tttgtctggc
     5221 caacgcgcga gcaacgctgg aaataccttt ctacggcgaa gctgaaagcc gcgccaggca
     5281 gtcaggcggc gtactctaac cttgcgttcg acctgctggc agatgcactg gcgaatgcgt
     5341 ccggcaagcc ttatacccag ttgtttgaag agcagattac ccatccattg gggatgaaag
     5401 acaccaccta caccccctca ccggatcagt gccgccgttt aatggttgct gagcgcggtg
     5461 ccagtccgtg caataacacg ctggcggcaa ttggcagcgg cggcgtctat tccacgcctg
     5521 gcgatatgat gcgctggatg cagcagtatt tgtcatctga tttttatcag cgcagtaacc
     5581 aggcagatcg catgcagacg ttgatttatc agcgcgcgca gtttacgaaa gtgattggca
     5641 tggatgtccc cggcaaagcg gatgcgcttg gtttaggctg ggtatatatg gccccgaaag
     5701 agggtcgtcc gaggattatt cagaagacag gcggtggtgg cggtttcatt acctatatgg
     5761 cgatgatccc gcagaagaat attggcgcgt ttgtggtggt cactcgttcg ccgttgacgc
     5821 gctttaaaaa tatgagcgat ggcatcaatg atctggtgac cgagctaagc gggaataaac
     5881 cgttggttat ccccgcatcc tgattaatac tctgaaggca aacggttgat agcgaccagt
     5941 ttgcctttat tcatttcgat atagccgcct ttacgcaaag cagcgagaac ttctgcgacg
     6001 acagaacgcg atatgcgtgt acgttgatgg atataattca tcacaccaat acgtgatcgc
     6061 aattcttcat tccagtcaat cattgataat aatgttgcgc gggtttgttt ggctggtaga
     6121 tgatatcccg tacaatttcc ttctgatatt aatttgtatg gtatatcgtt tttcattaaa
     6181 ccatcagcca gccccataat ataaggagcc tgggtaatac cgataagtac gttttcttct
     6241 ctacgcagag agataactcc ttctaaaata acgaaagtat cttcgttatt gacatcactg
     6301 gaaaatataa cttgcttttc attattaaac tcgaagcgcg taccgtatct ggacaaacat
     6361 ttatcgagct taccaaattc ctgaagaggt ttaactacag ataacatttg cgcgtccttt
     6421 gcagtaatgc ccgtcaaatc cttgacgggc attatttaga ttaaattacc agtatttctt
     6481 cggagtgaag aatattacca agtatattta acacccacgt tcgcggacca gtcttgatct
     6541 acgtcaccac caccgaggta gttagcatcg gtataggcgc tgaagttctt ggtgaagctg
     6601 aactgagtac ccagaccaac acgtaccgca gacccttcag taccgttatc gatggaatca
     6661 ccgttcacat cgttatcgtt gttagagtcg tcgtagacgt aagccagttt gaagtacgga
     6721 gtcagagcct gatcttcgct gtaggtgaag gtataacctg catctacacc cagttcataa
     6781 cgcatgctgt cgtaaggctg accgtcaact ttcatgtcgt tgctcagctg gtagtcatca
     6841 ccagactgga acagaccaga aatgctgccg taaggagtca cgtaaccagc atcacccagt
     6901 ttgaagtcgt aaccggtttt caaaccgaag ccccaagcgt cggagttggt gctaccgtca
     6961 acgtaagtac cgttgctcat ggttgcagac aggtcgttgt tgaagtgaga gtagctcaag
//
LOCUS       DAZXUK010000204         6910 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZXUK010000204 DAZXUK010000000
VERSION     DAZXUK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.204"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            202..606
                     /gene="sdhC"
                     /locus_tag="ACH3L8_003232"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             202..606
                     /gene="sdhC"
                     /locus_tag="ACH3L8_003232"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFT4086228.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            600..947
                     /gene="sdhD"
                     /locus_tag="ACH3L8_003233"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             600..947
                     /gene="sdhD"
                     /locus_tag="ACH3L8_003233"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFT4086229.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            947..2713
                     /gene="sdhA"
                     /locus_tag="ACH3L8_003234"
     CDS             947..2713
                     /gene="sdhA"
                     /locus_tag="ACH3L8_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFT4086230.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2729..3445
                     /gene="sdhB"
                     /locus_tag="ACH3L8_003235"
     CDS             2729..3445
                     /gene="sdhB"
                     /locus_tag="ACH3L8_003235"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFT4086231.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3746..6547
                     /gene="sucA"
                     /locus_tag="ACH3L8_003236"
     CDS             3746..6547
                     /gene="sucA"
                     /locus_tag="ACH3L8_003236"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFT4086232.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6562..>6910
                     /locus_tag="ACH3L8_003237"
     CDS             6562..>6910
                     /locus_tag="ACH3L8_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HFT4086233.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSP"
BASE COUNT         1602 a         1842 c         1915 g         1551 t
ORIGIN      
        1 ccggaacacc ctgcaatccc gagccaccca gcgttgtaac gtgtcgtttt cgcatctgga
       61 agcagtgttt tgcatgacgc gcagttatgg aaaggacgct gtctgacccg caagcagacc
      121 ggaggaagga aatcccgacg tctccaggta acagaaagtt aacctctgtg cccgtagtcc
      181 ccagggaata ataagaacag catgtgggcg ttattcatga taagaaatgt gaaaaaacaa
      241 agacctgtta atctggacct acagaccatc cggttcccca tcacggcgat agcgtccatt
      301 ctccatcgcg tttccggtgt gatcaccttt gttgcagtgg gcatcctgca gtggcttctg
      361 ggtaccagcc tctcttcccc tgaaggtttc gagcaagctt ccgcgattat gggcagcttc
      421 ttcgtcaaat ttatcatgtg gggcatcctt accgctctgg cgtatcacgt cgtcgtaggt
      481 attcgccaca tgatgatgga ttttggctat ctggaagaaa cattcgaagc gggtaaacgc
      541 tccgccaaaa tctcctttgt tattactgtc gtgctttcac ttctcgcagg agtcctcgta
      601 tggtaagcaa cgcctccgca ttaggacgca atggcgtaca tgatttcatc ctcgttcgcg
      661 ctaccgctat cgtcctgacg ctctacatca tttatatggt cggttttttc gccaccagtg
      721 gcgaactgac atatgaagtc tggatcggtt tcttcgcctc tgcgttcacc aaagtgttca
      781 ccctgctggc gctgttttct atcttgattc atgcctggat cggcatgtgg caggtgttga
      841 ccgactacgt taaaccgctg gccttgcgcc tgatgctgca actggtgatt gtcgttgcac
      901 tggtggttta cgtgatttat ggattcgttg tggtgtgggg tgtgtgatga aattgccagt
      961 cagagaattt gatgcagttg tgattggtgc cggtggcgca ggtatgcgcg cggcgctgca
     1021 aatttcccag agcggccaga cctgtgccct gctctctaaa gtcttcccga cccgttccca
     1081 taccgtttct gcgcaaggcg gcattaccgt tgcgctgggt aatacccatg aagataactg
     1141 ggaatggcat atgtacgaca ccgtaaaagg gtcggactat atcggtgacc aggacgcgat
     1201 tgaatatatg tgtaaaaccg ggccggaagc gattctggaa ctcgaacaca tgggcctgcc
     1261 gttctcgcgt ctcgatgatg gtcgtatcta tcaacgtccg tttggcggtc agtcgaaaaa
     1321 cttcggcggc gagcaggcgg cacgcactgc ggcggctgct gaccgtaccg gtcacgcact
     1381 gttgcacacg ctttatcagc agaacctgaa aaaccacacc accattttct ccgagtggta
     1441 tgcgctggat ctggtgaaaa accaggatgg cgcggtggtg ggttgtaccg cactgtgcat
     1501 cgaaaccggt gaagtggttt atttcaaagc ccgcgctacc gtgctggcga ctggcggagc
     1561 agggcgtatt tatcagtcca ccactaacgc ccacattaac actggcgacg gtgtcggcat
     1621 ggctatccgc gcaggcgtac cggtgcagga tatggaaatg tggcaattcc acccgaccgg
     1681 cattgccggt gcgggcgtac tggtcaccga aggttgccgt ggtgaaggcg gttatctgct
     1741 gaacaaacat ggcgaacgct ttatggagcg ttatgcgccg aacgccaaag acctggcggg
     1801 ccgtgatgtg gtggcgcgtt ccatcatgat tgaaatccgt gaaggtcgcg gctgtgatgg
     1861 tccgtggggg ccacacgcga aactgaaact cgatcacctg ggtaaagaag ttctcgaatc
     1921 ccgtctgccg ggtatcctgg agctttcccg taccttcgct cacgtcgatc cggtgaaaga
     1981 gccgattccg gttatcccaa cctgtcacta catgatgggc ggtattccga ccaaagttac
     2041 cggtcaggcg ctgactgtga atgagaaagg cgaagatgtg gttgttccgg gactgtttgc
     2101 cgttggtgaa atcgcttgtg tatcggtaca cggcgctaac cgtctgggcg gcaactcgct
     2161 gctggacctg gtggtctttg gtcgcgcggc aggtctgcat ctgcaagagt ctatcgccga
     2221 gcagggcgca ctgcacgatg ccagcgagtc tgatgttgaa gcgtctctgg atcgcctgaa
     2281 ccggtggaac aataatcgta acggtgaaga tccggtggcg atccgtaaag cgctgcaaga
     2341 atgtatgcag cataacttct cggtcttccg tgaaggtgat gcgatggcga aagggcttga
     2401 gcagttgaaa gtgatccgcg agcgtctgaa aaatgcccgt ctggatgaca cttccagcga
     2461 gttcaacacc cagcgcgttg agtgcctgga actggataac ctgatggaaa cggcgtatgc
     2521 aacggctgtt tctgccaact tccgtaccga aagccgtggc gcacatagcc gcttcgactt
     2581 cccggatcgt gatgatgaaa actggctgtg ccactccctg tatctgcctg agtcggaatc
     2641 catgacgcgc cgaagcgtca acatggaacc gaaactgcgc ccggcattcc cgccgaagat
     2701 tcgtacttac taatgcggag acaggaaaat gagactcgag ttttcaattt atcgctataa
     2761 cccggatgtt gatgatgctc cgcgtatgca ggattacacc ctggaagcgg aagaaggtcg
     2821 cgacatgatg ctgctggatg cgcttatcca gctgaaagag aaagatccca gcctgtcgtt
     2881 ccgccgctcc tgccgtgaag gtgtgtgcgg ttccgacggt ctgaacatga acggcaagaa
     2941 tggtctggcc tgtattaccc cgatttcggc actcaaccag ccgggcaaga agattgtgat
     3001 tcgcccgctg ccaggtttac cggtgatccg cgatttggtg gtagacatgg gacaattcta
     3061 tgcgcaatat gagaaaatta agccttacct gttgaataat ggacaaaatc cgccagctcg
     3121 cgagcattta cagatgccag agcagcgcga aaaactcgac gggctgtatg aatgtattct
     3181 ctgcgcatgt tgttcaacct cttgtccgtc tttctggtgg aatcccgata agtttatcgg
     3241 cccggcaggc ttgttagcgg catatcgttt cctgatcgat agccgtgata ccgagactga
     3301 cagccgcctc gacggtttga gcgatgcatt cagcgtattc cgctgtcaca gcatcatgaa
     3361 ctgcgtcagt gtatgtccga aggggctgaa cccgacgcgc gccatcggcc atatcaagtc
     3421 gatgttgttg caacgtaatg cgtaaactgt aggcctgata agacgcgcaa gcgttgcatc
     3481 aggcaaccag tgccggatgc ggcgtgaacg ccttatccgg cctacaagtc attacccgca
     3541 ggcctgataa gcgcagcgca tcaggcgtaa caaagaaatg caggaaatct ttaaaaactg
     3601 cccctgacac taagacagtt tttaaaggtt ccttcgcgag ccactacgta gacaagagct
     3661 cgcaagtgaa ccccggcacg cacatcactg tgcgtggtag tatccacggc gaagtaagca
     3721 taaaaaagat gcttaaggga tcacgatgca gaacagcgct ttgaaagcct ggttggactc
     3781 ttcttacctc tctggcgcaa accagagctg gatagaacag ctctatgaag acttcttaac
     3841 cgatcctgac tcggttgacg ctaactggcg ttcgacgttc cagcagttac ctggtacggg
     3901 agtcaaaccg gatcaattcc actctcaaac gcgtgaatat ttccgccgcc tggcgaaaga
     3961 cgcttcacgt tactcttcaa cgatctccga ccctgacacc aatgtgaagc aggttaaagt
     4021 cctgcagctc attaacgcat accgcttccg tggtcaccag catgcgaatc tagatccgct
     4081 gggactgtgg cagcaagata aagtggccga tctggatccg tctttccacg atctgaccga
     4141 agcagacttc caggaaacct tcaacgtcgg ttcatttgcc agcggcaaag aaaccatgaa
     4201 actcggcgag cttatttccg ccctcaagca aacctactgc ggcccgattg gtgccgagta
     4261 tatgcacatc accagcaccg aagaaaaacg ctggatccaa cagcgtatcg agtctggtcg
     4321 cgcgactttc aatagcgaag agaaaaaacg cttcttaagc gaactgaccg ccgctgaagg
     4381 ccttgagcgt tacctcggcg caaaattccc tggcgcaaaa cgcttctcgc tggaaggcgg
     4441 tgacgcgtta atcccgatgc ttaaagagat gatccgccac gctggcaaca gcggcacccg
     4501 cgaagtggtt ctcgggatgg cgcaccgtgg tcgtctgaac gtgctggtga acgtgctggg
     4561 taaaaaaccg caagacttgt tcgacgagtt cgccggtaaa cataaagaac acctcggcac
     4621 aggcgacgtg aaataccaca tgggcttctc gtctgacttc cagaccgatg gcggcctggt
     4681 gcacctggcg ctggcgttta acccgtctca ccttgagatt gtaagcccgg tcgttatcgg
     4741 ctctgttcgt gcccgtctgg acagacttga tgagccgaac agcaacaaag tgctgccaat
     4801 caccatccac ggtgacgccg cagtgaccgg gcagggcgtg gttcaggaaa ctctgaacat
     4861 gtcgaaagcg cgtggttatg aagttggcgg tacggtacgt atcgttatca acaaccaggt
     4921 tggtttcacc acctctaacc cgctggatgc ccgttctaca ccgtactgta ctgatatcgg
     4981 taagatggtt caggcaccga ttttccacgt taacgcggat gatccggaag ccgttgcctt
     5041 tgtgacccgt ctggcgctcg atttccgtaa cacctttaaa cgtgatgtct tcatcgacct
     5101 ggtatgctac cgccgtcacg gccacaacga agccgacgag ccgagcgcaa cccagccact
     5161 gatgtatcag aaaatcaaaa aacatccgac accgcgcaaa atctacgctg acaagctgga
     5221 gcaggaaaaa gtggcgacgc tggaagatgc caccgagatg gttaacctgt accgcgatgc
     5281 gctggatgct ggcgattgcg tagtggcaga gtggcgtccg atgaacatgc actctttcac
     5341 ctggtcgccg tacctcaacc acgaatggga cgaagagtac ccgaacaaag ttgagatgaa
     5401 gcgcctgcag gaactggcta aacgtatcag tacggtacca gaagcagttg aaatgcagtc
     5461 tcgcgttgcc aagatttatg gcgatcgcca ggcgatggca gccggtgaga aactgttcga
     5521 ctggggcggt gcggaaaacc tcgcttacgc cacgctggtt gatgaaggca ttccggttcg
     5581 cctgtcgggt gaagactccg gtcgcggtac cttcttccac cgccacgcgg tgatccacaa
     5641 ccagtctaac ggttccactt acacgccgct gcaacacatc cataacggtc agggcgcgtt
     5701 ccgtgtctgg gactccgtac tgtcagaaga agccgtgctg gcgtttgaat atggttatgc
     5761 caccgcagaa ccacgcaccc tgaccatctg ggaagcgcag ttcggtgact tcgccaacgg
     5821 tgcgcaggtg gttatcgacc agttcatctc ctctggcgag cagaaatggg gccggatgtg
     5881 tggtctggtg atgttgctgc cgcacggtta cgaagggcag gggccggagc actcctccgc
     5941 gcgtctggaa cgttatctgc aactttgtgc tgagcaaaac atgcaggttt gcgtaccgtc
     6001 taccccggca caggtttacc acatgctgcg tcgtcaggcg ctgcgcggga tgcgtcgtcc
     6061 gctggtcgtg atgtcgccga aatccctgct gcgtcatccg ctggcggttt ccagcctcga
     6121 agaactggcg aacggcacct tcctgccagc catcggtgaa atcgacgagc ttgatccgaa
     6181 gggcgtgaag cgcgtagtga tgtgttctgg taaggtttat tacgacctgc tggaacagcg
     6241 tcgtaagaac aatcaacacg atgtcgccat tgtgcgtatc gagcaactct acccgttccc
     6301 gcataaagcg atgcaggaag tgttgcagca gtttgctcac gtcaaggatt ttgtctggtg
     6361 ccaggaagag ccgctcaacc agggcgcatg gtactgcagc cagcatcatt tccgtgaagt
     6421 gattccgttt ggggcttctc tgcgttatgc aggccgcccg gcctccgcct ctccggcggt
     6481 agggtatatg tccgttcacc agaaacagca acaagatctg gttaatgacg cgctgaacgt
     6541 cgaataaata aaggatacac aatgagtagc gtagatattc tggtccctga cctgcctgaa
     6601 tccgtagccg atgccaccgt cgcaacctgg cataaaaaac ccggcgacgc agtcgtacgt
     6661 gatgaagtgc tggtagaaat cgaaactgac aaagtggtac tggaagtacc ggcatcagca
     6721 gacggcattc tggatgcggt tctggaagat gaaggtacaa cggtaacgtc tcgtcagatc
     6781 cttggtcgcc tgcgtgaagg caacagcgcc ggtaaagaaa ccagcgccaa atctgaagag
     6841 aaagcgtcca ctccggcgca acgccagcag gcgtctctgg aagagcaaaa caacgatgcg
     6901 ttaagcccgg
//
LOCUS       DAZXUK010000205         6910 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZXUK010000205 DAZXUK010000000
VERSION     DAZXUK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.205"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..694
                     /locus_tag="ACH3L8_003238"
     CDS             <1..694
                     /locus_tag="ACH3L8_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFT4086234.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="ACH3L8_003239"
     CDS             complement(1033..1803)
                     /locus_tag="ACH3L8_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086235.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2334)
                     /locus_tag="ACH3L8_003240"
                     /pseudo
     CDS             complement(1848..2334)
                     /locus_tag="ACH3L8_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2294..2515)
                     /locus_tag="ACH3L8_003241"
     CDS             complement(2294..2515)
                     /locus_tag="ACH3L8_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086236.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="ACH3L8_003242"
     CDS             complement(2516..3199)
                     /locus_tag="ACH3L8_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFT4086237.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="ACH3L8_003243"
     CDS             complement(3263..3574)
                     /locus_tag="ACH3L8_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086238.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4473)
                     /locus_tag="ACH3L8_003244"
     CDS             complement(3571..4473)
                     /locus_tag="ACH3L8_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFT4086239.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5872
                     /locus_tag="ACH3L8_003245"
     CDS             4913..5872
                     /locus_tag="ACH3L8_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFT4086240.1"
                     /translation="MLKVSCDDGSTNVKLAWFEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5872..6267
                     /locus_tag="ACH3L8_003246"
     CDS             5872..6267
                     /locus_tag="ACH3L8_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFT4086241.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6450..6830
                     /locus_tag="ACH3L8_003247"
     CDS             6450..6830
                     /locus_tag="ACH3L8_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFT4086242.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1888 a         1785 c         1675 g         1562 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac caggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggtttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaaatac acctatgatt tgggaagcac
     5101 tgctgtcata ggtacaacac atgtcagtta tcaatacagc accaccaatc tgcttgccat
     5161 acatcatgca ctgctgacat ccggccttca gccacaggat gtagaactga cagtaacatt
     5221 acctgtgacc gaattctttg ataatgacaa tcaaccaaat gaagaaagaa tagaaagaaa
     5281 aaaggcaaat gttctccgtg agatatctct gaataaaggg gaaacattta aaattaaaaa
     5341 agttaatgta atgcctgaat ctttgccagc tgcatttgaa tcactaaaaa aagataaagt
     5401 aaacaaactt gaacgcagcc tgattatcga tcttggagga accactctgg attgtggttt
     5461 aattctcggt gcatttgaag gcatttctga aatccgtggc tattcagaaa tcgggacatc
     5521 ccgcatcact cataccgtca tgaatgcgct gacgaaagcc tccacaccat gtaattattt
     5581 tattgctgat gagttaataa aaaacagaca cgataatgaa tatctccaga cattaataaa
     5641 tgatgtagct gaaataaaaa acatttctca tgtaatagac agagaagtga aatctctggc
     5701 agaaagcatc aggcaggaaa tttcaacatt ctcaggaatg aaccgaatct atctcactgg
     5761 cggaggagcc gagcttattt accctcacat aaagcaatat ttcccgaatc tgaaagtgaa
     5821 caaggtggat gaacctcagt ttgcattggt gaaagcaatg gtgcatgcgt aatggaaagc
     5881 agtgatccaa aaaaaagaaa gaaagttgtg gcatacctgc acccggcact ttatccgcag
     5941 gacaacctga cacaacaaac aatagactct ctcccagtac aaatgcgggg agatttctac
     6001 agacaatccc ttatttgcgg agcagctctt tattctgttg ccccccgact gttaacgcta
     6061 atcagcgttt ttttcagtga gaaaatcact gcagaaaatc tggtgaaact tatagaacaa
     6121 acaacaggat acacatccac atcaattgat ataagtgtgt taaaaaacat catagaggca
     6181 tcttcagaaa acaaatcaga gagcatcact tcaaaagatg atttcgaaga acaaaccaga
     6241 cgcaatcttt ctatgttaaa aaaataataa aagaagagca ggttcagtca attacctgct
     6301 cttcttttat acatactaaa aaatcagatt tttacgaata ttcagattat cataaaaaat
     6361 gcaacagaca ggaatgcaat aattttgcat tatctcgttg cttgtaatgc aaatatgcat
     6421 tattgtcagt gtgtgacaat aacagagaga tgacgatggc gaccattaac gcacgtattg
     6481 atgatgatat aaagaaccaa gcagatgaag tgctgaagct gatgaatatc agtcaaactc
     6541 aggctattgc agctttttat cagtatatta ctgaacaaaa gaaactgcca tttgtgatca
     6601 cctcaattgt aaaaacgccg catgatttat tacgtgaaag tacagacatg ttagctgaag
     6661 ctcttgcagt gatttccaat ctacaggtgt ggactgaaca gcaggatggg attggaaaag
     6721 caaagctaat ggagtattac cgccgcctcg acgcactgta ctgttgtgca aaagagaaaa
     6781 taggtcttct ttcagataac cgggatgctg aactaggctg tgtcccttaa tcatctgagt
     6841 tatagtaact gtctgtttac acaacatatt gaactatggc tcgctacgac cttcccgatg
     6901 aggcatggac
//
LOCUS       DAZXUK010000206         6787 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZXUK010000206 DAZXUK010000000
VERSION     DAZXUK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6787
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.206"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1741
                     /gene="espL4"
                     /locus_tag="ACH3L8_003248"
                     /pseudo
     CDS             1..1741
                     /gene="espL4"
                     /locus_tag="ACH3L8_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(1710..3447)
                     /gene="aceK"
                     /locus_tag="ACH3L8_003249"
                     /pseudo
     CDS             complement(1710..3447)
                     /gene="aceK"
                     /locus_tag="ACH3L8_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(3631..4935)
                     /gene="aceA"
                     /locus_tag="ACH3L8_003250"
     CDS             complement(3631..4935)
                     /gene="aceA"
                     /locus_tag="ACH3L8_003250"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFT4086243.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4965..6566)
                     /gene="aceB"
                     /locus_tag="ACH3L8_003251"
                     /pseudo
     CDS             complement(4965..6566)
                     /gene="aceB"
                     /locus_tag="ACH3L8_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
BASE COUNT         1729 a         1698 c         1625 g         1735 t
ORIGIN      
        1 cccaaaaccg tatagtatac cgacacattg ctgcacaata tattaatgat atttatcaga
       61 atgttgatta caaaccccat caagatgatt actcttcagc tgaaaaattt ctcactcact
      121 tcaacaaaaa atgcaaaaac cagactttgg cgttgatttc cagccgtcct gaggggcgtt
      181 gcgttgctgc ctgcggtgat ttcgggctag ttatgaaagc atattttgac aagatggaat
      241 caaatggcat cagtgttatg gcagccatat tactggtgga taaccatgct ttgacggtcc
      301 ggctaagaat aaagaacaca actgaaggat gtacccatta cgtggtttcg gtttatgatc
      361 ctaatgtaac taacgataaa ataagaatta tgagcgaaag caaagaggat attaaacact
      421 attctctgat ggattttatg aatgtagatt atagcctcct gaaatggtca aatgatcatg
      481 ttattaacca atctgttgca ataattccag cacttccgaa agaacagcta ttgatgttaa
      541 aaggaactgt ggatgaaata acccctccat tatcaccagc aacgatgaat ttgctaatgg
      601 caattggtca gaatcaccaa cttacgcaac tgatgattca gctccagaaa atgccagaac
      661 tacatagaac agaaatgttg actgcctata atagtattaa cctccccggt ttatatttgg
      721 ctataaatta tggcaatgcg gatatcgttg agactatttt caattcattg tcagaaacag
      781 gatatgaagg gttactctcg aaaaaaaatc tcatgcatat tctggaggca aaagataaaa
      841 atggtttttc tggattattt ttagcgatat cacgtaagga taaaaatgtt gtaacctcga
      901 ttctgaacgt cttacctaaa ctggccgcaa cacatcattt agataatgaa caggtgtata
      961 aattcctgag tgccaaaaat agaacgtcca gccatgtttt ataccatgtt atggcgaatg
     1021 gtgatgccga catgctgaaa atttttttgg tcgcgttacc tttgttaatt cgcacatgtc
     1081 atttgactaa agaacaggta ctcgatctcc tgaaggcaaa ggatttttat ggttgcccaa
     1141 gactatacct ggcgatgcaa aatggacata gcgatatcgt gaaagttatt ctcgaagcat
     1201 tgccctgtct ggcccaggaa attaacattt cagcttccga tattgtagat cttctgaccg
     1261 ctaaaagtct tgcgcgcgac acgggtttgt ttatggccat gcagcgcgga catatgaacg
     1321 ttattaatac tatttttaac gcattaccca ctctttttaa tacgtttaaa ttcgataaaa
     1381 aaatatgaag cccctcctcc tggcaaataa ttctaatgaa tatcccggtt tgttttcagc
     1441 gatacagcat aaacaacaaa acgttgtaga gacggtttat cttgctttat ctgaccatgc
     1501 acgcctgttt ggatttaccg ctgaagatat tatggatttt tggcaacaca aagcgccaca
     1561 aaaatactct gcctttgagt tggcttttga atttggtcac cgggttattg ctgaattaat
     1621 ccttaataca ttaaataaga tggctgaaag ctttggcttt acggataacc ctcgatacat
     1681 tgcggagaaa aattatatgg aagctttact caaaaaagca tctccccata ccgtacgcta
     1741 aatctttgcc tgcgccgata cgcataaaca tcttccacat gcccttcacg tatgcggttt
     1801 tgtagtgcgc gccagtaatc agcgcggaac aggtcggcgt gcatctcttc aaacagcgga
     1861 ccaatacgcg ggtcggcgca tagccagtgg cgaaactctt ccgggaaaac atcgcccggc
     1921 gagacgctgt accacggttc gctggcaagt tcgtcttccg gatagcgcgg cggcgggatg
     1981 tcgcggaaat tcacttccgt catgtagcaa atttcatcgt aatcataaaa aaccacacgc
     2041 ccgtgacggg tgacaccgaa gtttttaaac agcatgtcgc cagggaaaat gttagcagcg
     2101 gcaagctggc gaatagcgtt accgtattct tcaatggcgt cgcgcaactg ctgaccttcc
     2161 acttgttcca gccagatatt gagcggcacc atccgccgct cgattataga gatggcgaat
     2221 tacaatctgc tcaccaagat cggtgatttt ttccgctgct tcctggagca gtaattccat
     2281 taataccggg gaaatatgcc gcttctccag cacaaagttt tcaaactcct gggtgtccgc
     2341 cattcggccc acacggtcgt gctctttcac cagttgatag caggcacgaa cgtgagcggc
     2401 agacatctct ttctgcggcg cgaacttgtc tttgatgact ttgaataccc gatcaaagcc
     2461 cggcagcgta aacaccaaca tcaccattcc acgaataccc ggcgcttcaa tgaactgctc
     2521 attacagccc tgtagataaa cgagatattc gcggtagctt tcggttttgg cgtgcttctg
     2581 gcagccgata gccatataca attcagcagt ggttttacct ggcagaattt cccgtagcca
     2641 ctcgaccagc gctgcgggca gcggtgcata aaccataaaa taagaacgcg caaagccaaa
     2701 aacaatgctc gcttcggcgg tcgtcgtcag gcaggtatca ataaataact cgccgtcgtc
     2761 cgtctggtgg atcggcagta agaagggcaa cgtgccggaa ggcgtgatca gtttgcccac
     2821 cagccaggcg gctttattgc ggtaaaacag ttcgttcgcc acctgtaaat gactttccgc
     2881 gaggttgtct gtccccagcg tttccgtcag atggcgaatg atgtaatgaa tgtcacggct
     2941 tttattctgc cagcgcaggc gcagcggcag gtcgctgata acgcgcatca gtagagattc
     3001 ccagccgtga tcggggtgaa agtctttcgc cagcgggcgg ggaatggtac gaaagcggcg
     3061 ctctggctga gagctaaaga taaaaagccg ctcgggagta agcgagcggt ggtcaaataa
     3121 ccgacagtac acggagttaa aaaagctctc cgcaatctcg aagcgcgggt aatccggcaa
     3181 cagccgcgtg taatgctctt taacgcgcag taaaaatgcc gcgtccgtac tttggccgtt
     3241 agtaatgcag cgcagttgct ccacgaccag accaacgtga tgatcgtaaa gatggatacg
     3301 gtttttcatc gcctgctgga cagcatgcca gtcggcctgt tcgaaacgct gctgcgcccc
     3361 ggaagtcact tcgaggaatc gaccatactg agcgtcgaag ccttgcaaaa tggtttgagc
     3421 aatcagtaat tccaggccac gcggcattta gcgccctcat caggagcaga gaattgccgg
     3481 atgcggcgta aacgccttat ccagcctacg ttcggcaacg gttgtaggca tgataagacg
     3541 cgcaagcgtc gcatcaggca tcgtgcaccg attgccggat gcggcgtaaa tgccttatcc
     3601 ggcctacagt cagcaacgtt tgtaggttgc ttagaactgc gattcttcag tggagccagt
     3661 cagcgcggtg actgaagacg tgccgccctg aataatagtc gtcactttat cgaagtaacc
     3721 tgtacccact tcctgctggt gagatacgaa ggtatagcca tctttcgcgg cggcaaattc
     3781 cggctgctgc actttctcaa catagtgctt cataccttcg ccctgcgcat aagcgtttgc
     3841 caggtcgaac atgttaaacc acatgctgtg gatacctgct agggtgatga actggaactt
     3901 gtagcccata tccgacagct gctgctggaa gctggcaata gttttgtcgt cgaggttttt
     3961 ctgccagttg aacgacggcg agcagttata agccagcagt ttgcccggat atttcgcgtg
     4021 gatagcttgt gcaaagcgac gcgccagttc cagatccggc gtggaggttt cacaccagac
     4081 caggtcagca tatggcgcat acgccaggcc acggctgatc gcttgctcaa tgcccgcatg
     4141 agtacggaag aagccttcac tggtacgctc gccggtaata aattcgctgt catacgggtc
     4201 gcaatctgag gtgatcagat ccgccgcatc agcatcggta cgtgcaacca gcagggttgg
     4261 aacgcccgtc acgtcagctg ccagacgcgc cgcgaccagt ttctgaatag cttcctgagt
     4321 tggcactaaa actttgccgc ccatgtgacc gcatttcttc actgacgcca gctgatcttc
     4381 gaagtgaact gccgcagcac cggcttcaat catcgctttc atcagttcaa aggcattcag
     4441 gacaccgcca aaaccggctt ccgcatcggc aacgatcggc aggaagtaat cgacatagcg
     4501 cggatcgccc ggctcaatgc ccacggacca ttggatctga tcggcacgac ggaaggtgtt
     4561 gttgatccgc tccaccacag ctggcaccga gtttgccgga tagagcgact gatccggata
     4621 catgctggcc gccaggttag cgtccgccgc tacctgccat cccgacagat agactgcttc
     4681 aatacccgct ttcgcctgtt gcagcgcctg accgccagtc agtgcaccga ggttgttgat
     4741 gtagcctttt ttcgactcac cgtgcagcag acgccacatt ttcgctgcgc ccagttgcgc
     4801 cagcgtgcat tcaggattga ctgaaccgcg taatttcacc acatcttccg cactgtatgg
     4861 gcgagtaatg ccttcccaac gcggttgagt ccactctttc tgtaattctt caatttgttg
     4921 tgtacgggtt ttcatgtgca gatgctccat agttatgtgg tggtttacgc taacaggcgg
     4981 tagcctggca gggtcaggaa atcaattaac tcatcggaag tggtgatctg ttccatcaag
     5041 cgtgcggcat cgtcaaaacg gccctgggag aaacgttctt cgcccagttc gctggcaatg
     5101 actttcatct cttcgcccag catctggcgg aacaaggctt tggtcaccgg tttgccattg
     5161 ctcaacgttt tttgatgatg gatccactgc cagatcgagg tacgggaaat ttcagccgtc
     5221 gccgcatctt ccatcaggcc ataaatcggc acgcagccgt tgccggagat ccacgcttcg
     5281 atgtactgca cagccacgcg aatgttagcg cgcatacctt cttcggtgcg ttcaccatca
     5341 caaggtgcca gcagctgatc ggcagtaatc ggcgcgtctt gttcgcgcaa tacttcaagc
     5401 tgatttttac gggagccgag aatgtcgttg aataccgcca ttgccgtgtc cgcaaggcct
     5461 gggtgagcga tccatgtgcc atcgtgaccg ttattggctt ccagcgcttt atccgctttt
     5521 actttgttga gcacctggtt attacgctct tcatctttgc tcggaataaa cgcggccatg
     5581 ccgcccatcg caaaagcacc gcgtttatgg caggttttaa tcagcaggcg tgagtaagca
     5641 ttcaggaatg gtttatccat cgtcacggcc tgtctgtctg gcaggacacg atcgggatag
     5701 tttttcaacg ttttgatata gctgaagatg taatcccagc gaccgcagtt cagaccaaca
     5761 atatggtcgc gcagcgcgtg aaggatttcg tccatctgga acacggcggg cagcgtttca
     5821 atcagcaacg tcgccttgat ggtgccgcgc ggcagattaa agcgatcttc tgcatagctg
     5881 aagacttcgc tccaccaggc cgcttcctgc taggactggg ttttcggcag atagaaatag
     5941 ggaccgctgc cctttgccaa cagtgcctga tagttgtgga agaaatagag cgcaaaatca
     6001 aacaggctac cggggattgc ctcaccacgc caggtgacat gtttttccgg caagtgcaga
     6061 ccgcgtaccc gacaaatcaa aaccgctgga ttgggcttga gctgataaat tttgcctgct
     6121 tcattggtat agctgatggt accgttaacc gcatcgcgca ggttaatttg cccgtcgatc
     6181 actttgttcc agtctggtgc cagtgaatct tcgaaatcgg ccataaagac tttcacattg
     6241 gcgttcagtg cgttgatcac catcttgcgc tctaccgggc cagttatctc tacgcggcgg
     6301 tcttctaagt ccgcaggaat cccgcgaatt ttccaatcag catcgcgaat ggaagccgtt
     6361 tccgaaataa aatcaggcaa cgttccgtta tcaatatctt gctgctgctg aatgcgcgct
     6421 gccagaagtt tattgcgttg tggcgtaaaa tgcgtcacca gctcagtcag aaattctacc
     6481 gcttcggcag taagaatttg cttctcctgc tcgccatacg gccttgtgaa agccagttca
     6541 tcggttgttg ttgcctgttc agtcatcgtg cagctcctcg tcatggatcc gaaaacttcc
     6601 cctctgtgaa cgaaggaacg ttgtgcgctt ttcgttcagc acaactaaga ctactcattt
     6661 aaaatgcaaa atcaaaaaca atttccattt taattaaaaa tacttgataa cttattgata
     6721 acaatatgaa taaaatttat tcacttgctg aggtgcgttt cggaaataaa aaaggcaccc
     6781 gaaggtg
//
LOCUS       DAZXUK010000207         6722 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZXUK010000207 DAZXUK010000000
VERSION     DAZXUK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.207"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            81..584
                     /gene="dps"
                     /locus_tag="ACH3L8_003252"
                     /gene_synonym="pexB"
     CDS             81..584
                     /gene="dps"
                     /locus_tag="ACH3L8_003252"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFT4086244.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            988..1734
                     /gene="glnH"
                     /locus_tag="ACH3L8_003253"
     CDS             988..1734
                     /gene="glnH"
                     /locus_tag="ACH3L8_003253"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFT4086245.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            1873..2532
                     /gene="glnP"
                     /locus_tag="ACH3L8_003254"
     CDS             1873..2532
                     /gene="glnP"
                     /locus_tag="ACH3L8_003254"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFT4086246.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2529..3251
                     /gene="glnQ"
                     /locus_tag="ACH3L8_003255"
     CDS             2529..3251
                     /gene="glnQ"
                     /locus_tag="ACH3L8_003255"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFT4086247.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3512..5736
                     /gene="ybiO"
                     /locus_tag="ACH3L8_003256"
                     /pseudo
     CDS             3512..5736
                     /gene="ybiO"
                     /locus_tag="ACH3L8_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(5733..6659)
                     /gene="rlmF"
                     /locus_tag="ACH3L8_003257"
     CDS             complement(5733..6659)
                     /gene="rlmF"
                     /locus_tag="ACH3L8_003257"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFT4086248.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
BASE COUNT         1583 a         1744 c         1740 g         1655 t
ORIGIN      
        1 ccagaatagc ggaacacata gccggtgcta tacttagtct cgttaattac tgggacataa
       61 catcaagagg atatgaaatt atgagtaccg ctaaattagt taaatcaaaa gcgaccaatc
      121 tgctttatac ccgcaacgat gtctccgaca gcgagaaaaa agcaacagta gagttgctga
      181 atcgccaggt tatccagttt attgatcttt ctttgattac caaacaagcg cactggaaca
      241 tgcgcggcgc taacttcatt gccgtacatg aaatgctgga tggcttccgc accgcactga
      301 tcgatcatct ggataccatg gcagaacgtg ccgtgcagct gggcggtgta gctctgggga
      361 ccactcaagt tatcaacagc aaaaccccgc tgaaaagtta cccgctggac atccacaacg
      421 ttcaggatca cctgaaagaa ctggctgacc gttacgcaat cgtcgctaat gacgtacgca
      481 aagcgattgg cgaagcgaaa gatgacgaca ccgcagatat cctgaccgcc gcgtctcgcg
      541 acctggataa attcctgtgg tttatcgagt ctaacatcga ataaatccat cgctgatggt
      601 gcagaacttt agtacccgat aaaagcggct tcctgacagg aggccgtttt gttttgcagc
      661 ccacctcaac gcacttattt agtgcatcca tctgctatcc ccagctgatt aagtaaattt
      721 tttgtatcca catcatcaca caatcgttac ataaagattg ttttttcatc aggttttacg
      781 ctaaataatc actgtgttga gtgcacaatt ttagcgcacc agattggtgc cccagaatgg
      841 tgcatcttca gggtattgcc ctataaatcg tgcatcacgt ttttgccgca tctcgaaaaa
      901 tcaatgagtt gcaaaattgg cacgattttt tcatatatgt gaatgtcacg caggggatcg
      961 tcccgtggat agaaaaaagg aaatgctatg aagtctgtat taaaagtttc actggctgca
     1021 ctgaccctgg cttttgcggt ttcttctcat gccgcggata aaaaattagt tgtcgcgacg
     1081 gataccgcct tcgttccgtt tgaatttaaa cagggcgata aatatgtggg ctttgacgtt
     1141 gatctgtggg ctgccatcgc taaagagctg aagctggatt acgaactgaa gccgatggat
     1201 ttcagtggga tcattccggc actgcaaacc aaaaacgtcg atctggcgct ggcgggcatt
     1261 accatcaccg acgagcgtaa aaaagcgatc gatttctctg acggctacta caaaagcggc
     1321 ctgttagtga tggtgaaagc taacaataac gatgtgaaaa gcgtgaaaga tctcgacggg
     1381 aaagtggttg ctgtgaagag cggtactggc tccgttgatt acgcgaaagc aaacatcaaa
     1441 actaaagatc tgcgtcagtt cccgaacatc gataacgcct atatggaact gggcaccaac
     1501 cgcgcagacg ccgttctgca cgatacgcca aacattctgt acttcatcaa aaccgccggt
     1561 aacggtcagt tcaaagcggt aggtgactct ctggaagcgc agcaatacgg tattgcgttc
     1621 ccgaaaggta gcgacgagct gcgtgacaaa gtcaacggcg cgttgaaaac cctgcgcgag
     1681 aacggaactt acaacgaaat ctacaaaaaa tggttcagta ctgaaccgaa ataataacgc
     1741 tacacctgta aagcgcactg gcagttccct ctcccccctg gggagaggat tagggtgagg
     1801 ggcgcaaacc cgctccgggg ccattaatta ccctgaattt gattatttac accacggtaa
     1861 caggaacaac atatgcagtt tgactggagt gccatctggc ctgccattcc gcttctgatt
     1921 gaaggtgcca aaatgaccct gtggatttcg gtcctcggtc tggctggcgg tctggtaatc
     1981 ggattgctgg caggttttgc acgcaccttc ggtggttgga tagccaacca cgtcgcgctg
     2041 gtctttattg aagtgatccg cggcacacct atcgtcgtcc aggtgatgtt tatctatttc
     2101 gccctgccga tggcgtttaa cgacttacgc atcgacccat ttactgcggc ggtggtcacc
     2161 atcatgatca actccggcgc gtacattgcg gaaatcacgc gtggtgcggt gctgtctatc
     2221 cacaaaggtt ttcgtgaagc aggactggcg ctcggtcttt cacgttggga aaccattcgc
     2281 tacgtcattt taccgctggc actgcgtcgt atgctgccgc cgctgggtaa ccagtggatc
     2341 atcagcatta aagacacctc gctgtttatt gttatcggcg tggcggaact gacccgtcag
     2401 gggcaagaaa ttattgccgg taacttccgc gcccttgaga tctggagcgc cgtggcggtg
     2461 ttctatctga ttattaccct ggtgctgagc tttattctgc gtcgtctgga aagaaggatg
     2521 aaaatcctgt gattgaattt aaaaacgtct ccaagcactt tggcccaacc caggtgctgc
     2581 acaatatcga tttgaacatt gcccagggcg aagtcgtggt gattatcggg ccgtccggtt
     2641 ccggtaaatc gaccctgctg cgctgcatca acaaactgga agaaatcacc tccggcgatc
     2701 tgattgtcga tggcctgaag gtcaacgatc cgaaagttga cgagcgcctg attcgccagg
     2761 aagcaggtat ggtgttccag cagttttacc tcttcccgca tctgacggcg ctgggaaacg
     2821 tcatgtttgg tccgctacgc gtgcgtggcg cgaacaaaga agaggcggaa aaactggcgc
     2881 gtgagctgct ggcgaaagtc ggtctggcag aacgtgcaca tcactaccct tccgaacttt
     2941 ctggtggtca acagcagcgt gtggcgattg cccgcgcgct ggcggtgaag ccgaaaatga
     3001 tgctgtttga tgaaccgact tccgctcttg acccggaact gcgccatgaa gtgctgaagg
     3061 ttatgcagga tctggctgaa gaagggatga cgatggtgat cgtgacccac gaaatcggtt
     3121 ttgccgagaa agtagcttcg cggctgatct ttatcgacaa aggccggatt gcggaagatg
     3181 gcaatccgca ggtgttgatc aagaacccgc cgagccagcg cttgcaggaa tttttgcagc
     3241 acgtctctta ataagacaca ttgcctgatg cgctacgctt atcaggccta caggatatct
     3301 ggcaacttat taaaattgca tgaacttgta ggacggataa ggcgttcacg ccgcatccgg
     3361 caaaaaagcc cgcacgttgt cagcaacctg cttaatatcc cttcctccct ttcacccgaa
     3421 agggaggcac accagattcc tctcatttaa aatcgcccct cccccagcat ctatacttat
     3481 ctttttgctc tgttttctca ctggaggagt catgcggtgg atcctgttca tcctcttctg
     3541 cctgctgggc gcacctgccc acgcggtatc catacccggc gttacaacca caacgacaac
     3601 ggactcaacg actgaaccgg ccccggaacc ggatatcgaa caaaaaaagc ggcctatggc
     3661 gcactggcgg atgtgctgga taacgacacc tcgcgtaaag agttgatcga ccagttgcgt
     3721 actgtcgccg cgacgccccc tgcggaaccg gtcccgaaga tcgtgccgcc gacgctggtt
     3781 gaagagcaaa ccgtgctgca aaaggtcacc gaagtcagcc gccattatgg tgaagccctt
     3841 tccgcccgct tcgggcaact ttatcgcaat atcaccggct ccccgcataa gccatttaat
     3901 ccacaaacct tcagcaatgc gctgagccat ttttcaatgt tagcggtatt agtgtttggt
     3961 ttttactggc tgattcgcct gtgcgcactg ccgctatatc gcaaaatggg tcagtgggcg
     4021 aggcaaaaaa atcgtgagcg cagcaactgg ttgcagcttc cggcgatgat tattggggcg
     4081 tttattatcg acctgctgtt actggccctg acattatttg tcggccaggt attaagcgac
     4141 aacctgaatg caggcagtcg caccatcgct ttccaacaaa gtttgtttct caacgccttt
     4201 gccctgattg aatttttcaa agccgtacta cgcctgattt tttgcccaaa cgtggcggag
     4261 ctgcgcccgt tcacgattca ggacgagagc gcccgttact ggggtcgtcg cctgagctgg
     4321 ttaagcagcc tgattggcta tggcctgatt gtggccgtgc cgattatctc taatcaggtg
     4381 aatgtacaga taggtgcgct ggcgaacgtc atcattatgc tgtgcatgac cgtctgggcg
     4441 ttgtacctga tcttccgaaa taaaaaagag attacccagc atatgctcaa ctttgcggag
     4501 cattcgctgg cttttttcag cctgtttatc cgcgcctttg cgctggtgtg gcactggctg
     4561 gcaagcgcct attttatcat gctgtttttc ttttcgttgt tcgatccggg caacagtctg
     4621 aaatttatga tgggtgcaac ggtgcgcagc ctggcgatta ttggtatcgc agcgtttgtt
     4681 tccggtatgt tttcccgctg gctggcgaaa accatcaccc tctcgccaca tactcagcgt
     4741 aactatccgg agctacaaaa acggctgaat ggctggcttt cggcggcgct gaaaacggcg
     4801 cgtattctga ccgtctgcgt ggcagtaatg ttgctgttga gcgcatgggg attgttcgat
     4861 ttctggaact ggctgcaaaa cggcgcgggg cagaaaaccg tagatatcct gatccgcatc
     4921 gcactcattc ttttcttctc ggcggttggc tggacggtgc tcgccagttt gatcgaaaac
     4981 cggctggctt cggatattca tggccgcccg ctacccagcg cccgtacgcg taccctgctg
     5041 acgctgttcc gtaacgcgct ggcggtgatt atcagtacca tcaccatcat gattgtgttg
     5101 tcggaaatcg gcgtcaatat cgcgccattg ctggcaggtg ccggggcatt aggtctggct
     5161 atctcgtttg gttcgcaaac gctggtaaaa gatattatca ccggggtatt tattcagttt
     5221 gaaaacggca tgaacactgg agatttggtg actatcgggc cgttgaccgg cacagtggaa
     5281 cggatgtcga ttcgctccgt gggcgtgcgc caggataccg gggcgtatca catcattccg
     5341 tggtcttcga taaccacctt tgctaacttc gtccgcggta ttggttcggt agtggcaaac
     5401 tacgatgttg atcgccatga agatgctgat aaagccaatc aggcactgaa agatgcggta
     5461 acggaattga tggaaaacga agaaattcgc gggctgatta ttggtgaacc gaactttgcc
     5521 gggattgtcg gcttaagcaa taccgcgttt acactgcgtg tttcgttcac cacgctgcca
     5581 ctcaaacagt ggacggtacg ctttgccctc gacagccagg tgaaaaaaca tttcgacctg
     5641 gcgggcgttc gcgcgccagt gcagacttat caggtgctgc ctgctccggg cgcgaccccg
     5701 gctgaaccgc tgccgccggg ggagccaacg ctttaacgtt ggcgattaac aaaacggcgg
     5761 cgctgctcgt cgttcataaa ggtccaggca ataaagcgac tctgcttttg cccctgggcc
     5821 atctcttttt ttaccacctt caccgcgccc acgtcggtca gagcacgata caacggcggt
     5881 aagttttcac cacgagatac cagcgaggta aaccacatca cctgcttcgc gaagcctttg
     5941 ctctcttcaa tcatcttttt gataaagttg acttcaccgc cttcacacca caactcctgt
     6001 tgctgaccac caaagttcag tgcatcgtct ttgttcagcc ccaggttacg gcgtttacgc
     6061 tcactacctg cccgcgccgc agcggcggaa tcgtggaatg gcgggttaca caaggtcgcg
     6121 tcgtattgct cgtttttgtg gatgatgccg ttaaaaatcg ccccactctc tttttgccga
     6181 cgcagacgaa tggcgcggtt aagccccgga ttagcactga taatcgcctg cgcactgctt
     6241 aacgcctggc tgctggtttc actaccggta aaacgccagc catattcatg tacgccaatc
     6301 agcggataaa tacagttcgc accaacgccg atatccagaa tgctggcatt cgctggaatt
     6361 gttccgcttg cttcagccag taaatcggca aggtgatgaa tataatccgc ccggcccggt
     6421 actggtgggc agagaaaacc gtcggggata tcccagttcg ctacggcgta aaaatgggcc
     6481 agcaacgcct tattgagcgc cttcaccgcc agcggattgg caaagtctac gctttgctcc
     6541 cctgcgggtg taagcgtgag gaattgcctg agttcaggat tgacctgaca aagcgtggcg
     6601 agatcgtagc ggctgtgatg acggttgcgc ggatgcaacc ccggtttctg ggcggacata
     6661 gcattctcct ttaagcagcg gcgtaagata cccgcataac gcgacagggt aaatatctgg
     6721 ct
//
LOCUS       DAZXUK010000208         6633 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZXUK010000208 DAZXUK010000000
VERSION     DAZXUK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.208"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..236)
                     /locus_tag="ACH3L8_003258"
     CDS             complement(<1..236)
                     /locus_tag="ACH3L8_003258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086249.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEG"
     gene            complement(325..1203)
                     /gene="narH"
                     /locus_tag="ACH3L8_003259"
                     /pseudo
     CDS             complement(325..1203)
                     /gene="narH"
                     /locus_tag="ACH3L8_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
     gene            complement(1200..4940)
                     /gene="narZ"
                     /locus_tag="ACH3L8_003260"
                     /pseudo
     CDS             complement(1200..4940)
                     /gene="narZ"
                     /locus_tag="ACH3L8_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            complement(5022..6410)
                     /gene="narU"
                     /locus_tag="ACH3L8_003261"
     CDS             complement(5022..6410)
                     /gene="narU"
                     /locus_tag="ACH3L8_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFT4086250.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
BASE COUNT         1582 a         1738 c         1749 g         1564 t
ORIGIN      
        1 ccttcgtcat caacacgtac aaaccagaag accagctttt tgtttcccgc atccacaaag
       61 aacggaatat tcaggtctgc gcagcattca acggcatcgt caaaactatc aaagcgcaga
      121 acttctgcgt cttcttcgtc aaaaaaatca tcttcgtgaa gcttcacgac atagcgggga
      181 agtttgcttc tttgagaggc gggtttacgt ttacggggtt tagctgaacg ggccatataa
      241 ccacctgaaa gacaatgaca ttgcctgttt ttataacggt aattgcagac catgacaagc
      301 cgcagccgtc aggctgccta ctcgatagag gtcaacttcg cgctcggtgc tcgacgcttc
      361 ctcgatgcgg tcggcgtcgt aaagcagcac gcccagatac cggatgcgac ccacgcaggt
      421 ttctgagcac acggtgggtt ggcctgactc aattcgcgga taacagaaga tacatttttc
      481 tgacttgccg cttttccagt tgaagtagat ttttttgtac ggacaaccgc taatgcacaa
      541 acgccagcca cggcatttat cctgatcaat cagcacaatg ccgtcttctt cgcgtttgta
      601 gatagcgccg cttgggcagg tcgccacgca gctgggattg aggcagtgtt cgcacaggcg
      661 cggcaggtac atcatgaagg tgttttcaaa ctgcccgtac atctcctttt gcatggcctc
      721 gaagttgcgg tcgcgggcac gtttttcaaa ctcgccgccc agcagttctt cccagtttgg
      781 gccccagatc actttgtcca tccgtttgcc gtcaatcagt gaacgcgggc gagcagtagg
      841 gatatgtttg ccttccggtg tgctatgcaa atgttcgtag tcgaaggtga aaggttcgta
      901 gtaatcatca atctgctgcg gcaccaccgg gttggcgaag attttggtta ttacgcccat
      961 cttgctgccc agacgcggac ggatcttgcc attcacatcg cggacccagc cgccttgcca
     1021 ctcttcctga tcttcccagt ttttcggata accaatgccc ggtttggttt cgacgttgtt
     1081 aaaccatgcg tactccatgc cttcgcgtcc ggtccagacg ttgttacagg tcaccgaaca
     1141 ggtatggcag ccgatacatt tatcgaggtt aagcaccatg ccgacttgcg aacgtatttt
     1201 cattttttcg cctcctgtac ctgatcgcga ccttcatcat ccagccagtt aacgttcttc
     1261 atcttgcgga tcatgatgaa ttcgtcgcgg ttcgaaccga cggtgccgta gtagttaaag
     1321 ccccaggcca gctgcgcgta accgccaatc atatgcgtcg gtttcgggca aacgcgggtg
     1381 accgagttat gaatgccgcc gcgcatgcca gttacttccg aaccaggaat attcataatg
     1441 cgttcctggg cgtgatacat catggtcatg cccggcggta cacgttggct gaccaccgcg
     1501 cgggcagtca gcgcgccgtt ggcgttaaat acttccaccc agtcgttatc gacaatggtc
     1561 agttctcggg catctgtttc gctgatccag acaatcggtc caccgcgaga gagcgtcagc
     1621 attagcaggt tttcactgta ggttgagtga atgccccatt tctggtgcgg cgtcaggaag
     1681 ttaagtgctt tttcctggaa gccgtttggc ggtatctggc gcatctcact gacgctacgg
     1741 gtgtcgatag gcgggcgata agccaccagg ctttcaccaa aagcacgcat ccacggatga
     1801 tcctgataga gctgctggcg tcccgacagc gtgcgccacg gaattaactc atgaacgttg
     1861 gtgtatcccg cgttatagga tacgtgatcg ctttccagac cggaccatgt ggggctggag
     1921 ataattttac gcggctgcgc ctgaatatcg cgaaagcgaa tcttctcgtc ctctttgtgc
     1981 agcgccagat gggtatgttc gcgcccggtg atctcgccca gcgcctgcca cgctttgact
     2041 gcaacatgac cgttggtttc cggtgccagt gccagaatca cttctgaagc gtcaatggcg
     2101 gtgtcaatca gcggacgccc ctgtgccggg ccatcacgct tggtgtaatt gagtttgccg
     2161 aggaaatcga tttcatcctg ggtattccac gaaatccctt taccgccatt gccaagtttg
     2221 tccatcaatg gcccgagcga ggtaaagcgt tcatacgtag cagggtagtc gcgctccacc
     2281 gccacaatat tcggcgcggt tttacccggg atcagatcgc attcgccttt gcgccagtcg
     2341 agcacttcac acggctgtga gagctctgcc ggagagtcat gcagcagtgg ttgtaatacc
     2401 acgtcggttt ctttgccaag atggcccacg cacacttgcg aaaatgcttt ggcaatacct
     2461 ttgtagattt cccagtcgct gcgtgattcc caggccggat cgaccgccgc agaaagcgga
     2521 tgaataaatg gatgcatatc cgaggtgttc atatcgtctt tttcatacca ggtggcggtg
     2581 ggcagaacga tatcggagaa caggcaggta ctggacatgc ggaagtcgag cgtcaccagc
     2641 aggtcgagct tgccttcaat cgccgcagtt tgccactcga cttcttccgg tttgatcccg
     2701 tcgctggcac cgagttcctc gccctgaatc ccgctttcgg tccccagcag atacttctgt
     2761 atatactcgt ggcctttgcc ggaggagcca agcaggttag agcgccagac aaacaaatta
     2821 cgcggatgat tgctgccgct atctggttgt tcgcaggcca tacgtaaatc gcccgatttc
     2881 agcgcctggg cggtaaattc tgtgggggat aatccggcct tgtcggcttc agctttaatc
     2941 ccgagcgggt tacgccccag ctgcggcgca gaaggtagcc agcccatacg ttcggcgcga
     3001 acgttgaaat caatcagatg accgctgtac ttactggcat cggcgagcgg tgaaagtaac
     3061 tcctgagcag agactttttc atagcgccat tggctggaat gattgtagaa aaacgaggtg
     3121 ctgttcatct ggcgcggtgg tcggttccag tcgagcgcaa aggccagtgg caaccagccg
     3181 gtttgtgggc gcagtttttc ctggccgaca tagtgtgccc agccgccacc gctttgcccg
     3241 acacaaccgc agaagatcag catattgatc atcccgcggt agttcatgtc catgtgatac
     3301 cagtggttaa cacctgcgcc gaggataatc attgagcgcc catgcgtttt atgggcggta
     3361 tcggcaaatt cgcgggcgat ggtttcaata tactggcgcg gtacgccggt aatttgctca
     3421 ccccaggctg gggtgtacgg tttgatttca gcgtaatctt tcgcgctgtt ttcatcttcc
     3481 agcccgcgat cgaggccgta attcgccagt accaaatcat aaacgctgac caccggacag
     3541 gtgttgccat cgactaacgt caggttttta acgggcaatt ggcgcaccag caccggattg
     3601 tgttttacgc tgcgaaaatg cggattttca atgccgccaa agtaggggaa ggccacgcct
     3661 gcaacagcgt catgttgacc gagcagggtt aacgacaatt cggtttccgt accggcggca
     3721 atggattcca gattccattt gcctttttct ccccagcgga aaccaatcga accgttcggc
     3781 actaccaatt cacctgcggt attaactgct acggttttcc actgcggatt gttgctttcg
     3841 cccagtccat ccaccaggtc agatgcgcgg atcatgcgcc cgggaacgta gctaccatcg
     3901 tcgcgaggct ccagcattac cagcatcggc atgtcgctgt agcggcggca gtagttgata
     3961 aagtagtcgc tgggattatc gagatgaaac tcttttaaaa tcacatgccc cattgccatc
     4021 gccagggcgc tatcagtgcc ttgtttcggt gccagccact ggtcgcacaa tttggccact
     4081 tcagagtagt caggggtaat ggcgatggtt ttagtgcctt tgtagcgtac ttcggtaaag
     4141 aagtgggcgt ccggcgtacg tgtctgcggt acgttagacc cccaagcgat gatgtagctg
     4201 gagttatacc agtcggcaga ttccggtacg tcggtttgct cgccccaggt catcggcgag
     4261 gcgggcggca ggtcgcaata ccagtcgtag aaactttaac aggtgccgcc aagcagcgac
     4321 agataacgcg ttccggcggc gtaagaaacc atcgacatcg ccgggatcgg cgagaaaccg
     4381 gcaacgcgat ccgggccgta ggttttgatg gtccagacgt tagcggcggc aatcagctgg
     4441 tttagttcct gccagttgga gcggataaac ccgccgcgcc cacgcacttg tttgtagctc
     4501 aggcactttt gcggatcatt cataatcgat gcccacgcca gtaccggatc gctgtgttgt
     4561 ttgagggctt cgcgccacag ttcaatcagt cgtttacgaa tgagcgggta tttcaggcgg
     4621 ttagcgctgt aaagatacca ggagtaactt gcgccacgcg ggcagccgcg aggttcatga
     4681 ttgggcaggt cagggcgagt gcgcgggtag tcggtctgtt ggatttccca ggtcaccaga
     4741 ccatttttaa cgtagatttt ccagctacag gagcctgtac agttaacgcc gtgggtggaa
     4801 cgcacgattt tgtcgaactg ccaacgctgg cgatagctgt cctcccagtc gcggttgcta
     4861 tgcatcacct gtccgtgacc atcggcaaag gtttcgccct tttgtttgaa gtagcgaaag
     4921 cgatccaaaa gtttactcat gacatgactc ctgctccagg aatgaattgg gatctgatgc
     4981 cttgtcggat gcgacatggt tgtacatcag gcatcgatct cttatttttg gctgaacttc
     5041 cgccgaccat aaaccagcca ggtcagcagc acacaaacga tgtagaagat taaaaacact
     5101 ttcatcgcgc cgaccggaga gccggtcata ttgagcgaca tgccaaatgc ctgcggaata
     5161 aaaaagccgc ccactgcgcc aatggctgag ataaagccca gcgttgccgc cgtttcggtg
     5221 acggcttctc tctgagcttg ctcatcacta ccgcctttca tcttcacccg ataaatggtt
     5281 atctggcgaa agatgacggc gatcatctgg aaagtagaac cacttcccag acccgcggtc
     5341 agaaacagcc ccataaatac ggcgtaaaag gcgatgaaat taccggagcc tgtgcccggt
     5401 aaggtaagga acagcagggc actgaaaatc gccataaaaa taaagttgat caacgtcacc
     5461 cgcacgccgc cgaatttatc ggaaatagca ccaccaaccg accgcgcgat ggcaccaata
     5521 aatgggccaa agaacgccag gcgcagaata ttcacatccg ggaactgggt ttttgccagc
     5581 atggcaaaac ccgcagaaaa accgataaac gaaccgaagg tggcaaggta aagcaggctc
     5641 agcagccaga gatgcaggcg ttgtaagaca gggagctggt cggcaattga ggcgcgtgaa
     5701 ctggcgatat cattcatccc tgaccaggcg gcgatcgtgg caatcgccag tagcggtacc
     5761 caaatccatg cggcattcgc cagcgacatc accgaaccgt cggcctgcgg tacgccattg
     5821 acgccgagaa aggcaaatac aggtacaaaa atgaccagcg gtgcaaccag ctgcattaca
     5881 cttacaccta agtttcctaa tccgccatta atcccaagag cgctcccttg tttggctttt
     5941 ggaaagaaga aactgatatt gcccatgctc gaagcaaagt ttgcacctgc aaaaccgcat
     6001 agcaaagcga taacgataaa tatcccaaaa ggagtattcg gattttgcac ggcaattccg
     6061 agccagacgc aaggaataat caggattgca gtactaaaaa ccggccatcg gcgtccaccg
     6121 aatataggca ccataaagga gtagggaaca cgcaataatg cgccagaaac ggagggtaat
     6181 gcggttaata aaaagagttg atcggtagta aaattaaaac cgattttatt gagattaacg
     6241 gtaactgcgc taaatagcat ccagacacag aaggcaagaa gtagacaact gactgatatc
     6301 cagaggtttc ttcgagcaat atgttttcct ttattttccc agaaggccgg attttctggt
     6361 ttccagtcgc gtaaaagata acgactattt ttctcatttt gcagtgccat attgttcctc
     6421 atatgcacac attggtaatg aaaaaagaca aaacaggagg taaggcgcaa tagccagtta
     6481 ttagaattaa ggatgaattg ggtgaagtgc tgattgaaag aatagataag aaagcgtaac
     6541 cgcgggggca gaatggggat taagtagcca gatatgtgtt actaaatgta actaacaagc
     6601 taaccttcaa acgggggtta atctttgaac gta
//
LOCUS       DAZXUK010000209         6609 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZXUK010000209 DAZXUK010000000
VERSION     DAZXUK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.209"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            28..879
                     /gene="hchA"
                     /locus_tag="ACH3L8_003262"
     CDS             28..879
                     /gene="hchA"
                     /locus_tag="ACH3L8_003262"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFT4086251.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(987..2345)
                     /gene="hprS"
                     /locus_tag="ACH3L8_003263"
     CDS             complement(987..2345)
                     /gene="hprS"
                     /locus_tag="ACH3L8_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFT4086252.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="ACH3L8_003264"
     CDS             complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="ACH3L8_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFT4086253.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3149..3562
                     /gene="hiuH"
                     /locus_tag="ACH3L8_003265"
     CDS             3149..3562
                     /gene="hiuH"
                     /locus_tag="ACH3L8_003265"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFT4086254.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3671..4675
                     /gene="msrP"
                     /locus_tag="ACH3L8_003266"
                     /pseudo
     CDS             3671..4675
                     /gene="msrP"
                     /locus_tag="ACH3L8_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4676..5311
                     /gene="msrQ"
                     /locus_tag="ACH3L8_003267"
     CDS             4676..5311
                     /gene="msrQ"
                     /locus_tag="ACH3L8_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFT4086255.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5569..6219
                     /gene="zinT"
                     /locus_tag="ACH3L8_003268"
     CDS             5569..6219
                     /gene="zinT"
                     /locus_tag="ACH3L8_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFT4086256.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1852 a         1508 c         1388 g         1861 t
ORIGIN      
        1 taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc
       61 gatattgctg aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt
      121 cccgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt
      181 ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc
      241 ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa
      301 ttcgaagtgg cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac
      361 aaagatgaaa aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag
      421 aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt
      481 cctggtgggc atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta
      541 cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt
      601 ctggcacttc gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac
      661 gccgcagaca aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc
      721 ggcgaagagt tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta
      781 cataaggacc gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa
      841 ctggcggcgc aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta
      901 agcgcataat caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt
      961 aacttatggc gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg
     1021 ttatgcttac tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg
     1081 actaaagaaa ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat
     1141 ctacggaaga gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta
     1201 agagagccgt tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat
     1261 cgaatggcgt taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag
     1321 atttgttgat tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga
     1381 tagtccaaaa gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca
     1441 ttgtttttgt ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc
     1501 agctcttcaa tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg
     1561 agcgtaacct gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga
     1621 tcgtcagcaa attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc
     1681 aacgcctgcc caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg
     1741 ctatcgttat aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc
     1801 gtcctgatta acaacggact taatactgag cacagtataa tggcgacaat gcaaattata
     1861 atgctattaa ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca
     1921 gtaaccgtta atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta
     1981 tcattaataa tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta
     2041 tttaacatgt catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga
     2101 atgatcaaaa tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct
     2161 ggatttaccc catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta
     2221 tcatcgcgcc atttcaactc acttgccagg ccattataga gagtccatac aattccagcg
     2281 ccagcaacag actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt
     2341 ttcattattt ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct
     2401 tttcaggaaa aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat
     2461 tggtatcact atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa
     2521 tctcgccagc tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa
     2581 tactgatatt gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt
     2641 ctaatgttga attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact
     2701 cagaaaatga aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc
     2761 tgtcatcgac agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta
     2821 acgtttgtaa gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg
     2881 cataatcatc cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga
     2941 cataacccgc ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa
     3001 taagtagaat cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt
     3061 tcattacaaa attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa
     3121 aactcattca catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc
     3181 aacggcagcc ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt
     3241 gcacattttg aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa
     3301 gaaagcggat aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat
     3361 taaggcactg tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac
     3421 cggggactat ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt
     3481 tcatattaat aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta
     3541 ttcaacctat cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag
     3601 ggaggtgact ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt
     3661 gtgatggctt atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt
     3721 attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc
     3781 tttgcctcac gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc
     3841 cgcccccgcc ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc
     3901 actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa
     3961 agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag
     4021 cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct
     4081 ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat
     4141 tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt
     4201 cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg
     4261 cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct
     4321 cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt
     4381 gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa
     4441 gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt
     4501 ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt
     4561 tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta
     4621 cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg
     4681 tctgacagca aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc
     4741 gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga
     4801 tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac
     4861 acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt
     4921 atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt
     4981 gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat
     5041 tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa
     5101 actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc
     5161 aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct
     5221 ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg
     5281 caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat
     5341 cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt
     5401 tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca
     5461 atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt
     5521 acaatataac attacacata atgtacatta actttggagg aaattgtttt ggcgattcgt
     5581 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5641 ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5701 tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5761 gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca
     5821 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat
     5881 atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5941 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     6001 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6061 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6121 tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6181 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6241 tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga
     6301 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac
     6361 tgagaaaaag gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt
     6421 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
     6481 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
     6541 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6601 ttggctcaa
//
LOCUS       DAZXUK010000210         6583 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZXUK010000210 DAZXUK010000000
VERSION     DAZXUK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.210"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..577)
                     /gene="yeeO"
                     /locus_tag="ACH3L8_003269"
                     /pseudo
     CDS             complement(1..577)
                     /gene="yeeO"
                     /locus_tag="ACH3L8_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            738..813
                     /locus_tag="ACH3L8_003270"
     tRNA            738..813
                     /locus_tag="ACH3L8_003270"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:771..773,aa:Asn,seq:gtt)
     gene            complement(851..1801)
                     /gene="cbl"
                     /locus_tag="ACH3L8_003271"
     CDS             complement(851..1801)
                     /gene="cbl"
                     /locus_tag="ACH3L8_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFT4086257.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1903..2820)
                     /gene="nac"
                     /locus_tag="ACH3L8_003272"
     CDS             complement(1903..2820)
                     /gene="nac"
                     /locus_tag="ACH3L8_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFT4086258.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3148..3223
                     /locus_tag="ACH3L8_003273"
     tRNA            3148..3223
                     /locus_tag="ACH3L8_003273"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3181..3183,aa:Asn,seq:gtt)
     gene            complement(3279..4214)
                     /gene="ldtA"
                     /locus_tag="ACH3L8_003274"
     CDS             complement(3279..4214)
                     /gene="ldtA"
                     /locus_tag="ACH3L8_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFT4086259.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(4276..5355)
                     /gene="cobT"
                     /locus_tag="ACH3L8_003275"
     CDS             complement(4276..5355)
                     /gene="cobT"
                     /locus_tag="ACH3L8_003275"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFT4086260.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5367..6110)
                     /gene="cobS"
                     /locus_tag="ACH3L8_003276"
     CDS             complement(5367..6110)
                     /gene="cobS"
                     /locus_tag="ACH3L8_003276"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFT4086261.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6107..>6583)
                     /gene="cobU"
                     /locus_tag="ACH3L8_003277"
     CDS             complement(6107..>6583)
                     /gene="cobU"
                     /locus_tag="ACH3L8_003277"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFT4086262.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         1778 a         1771 c         1446 g         1588 t
ORIGIN      
        1 gtattttcgt attccctgca ccacgaagtg ccccgctacc aataagagtg atggcagcgg
       61 ctggataact gagtacggtc agctccagat aagtcaacgc cagtgcttta acttctgtcg
      121 tggcatcacc cgcgacgaaa tcaataattt gttcgccaaa atgatgaatt agcgttgcca
      181 acagtacggc aaacaacgtc atgatcacca atgactgccg cgtcgccacc ctcgctcgtc
      241 gtcgatcccg cttaccgaga ctaaatgcta caacgacagt agtaccaaga tcgatagctg
      301 caaaaaaagc cataatgacc atattgaagc tgtccgccaa ccccacgccg gccatcgcat
      361 cttttcccag ccaactgacc agaaaagtgc tcaggacccc catcaacagg acacaggcgt
      421 tctccatgaa gataggaaca gcaagcgggg ttatctcgcg ctagaacaac actttgtagc
      481 tcttgcgttt agagtgccag cgagtgccgt gaacaacctg gcgtaaagca gaggagatat
      541 tcaaagccga ccttaattgc agaaagtgaa accacatttc aaatgatgag ggagaatcag
      601 caaagctgca aagattttcg ccaacaaatt gtctgcaaat gcaacaaact gttgatagaa
      661 acggcaaaca gttgggaaat ttaaaaatcg ggtttgacaa aatatttttc gccgttaaga
      721 tgtgcctcaa cagcgattcc tctgtagttc agtcggtaga acggcggact gttaatccgt
      781 atgtcactgg ttcgagtcca gtcagaggag ccaaatttct gaaaagcccg cttttatagc
      841 gggctttttg ctatatctga taatcaattt cctcttcact gttttccatc acctggcgct
      901 tgatatcctc aactgacagt cctgcattac aaagttccag aaagcgccaa acatagttac
      961 gctgaagttg tcctcgcttc agtcccaacc agacagtatt agcatcgaaa agatgccgcg
     1021 tatccaggcg gattaaattc tcttcctctt gttcgccgct ggattgttcg gcaactaatc
     1081 cgatcccaag cccaagagca acataggttt taatgacatc agaatcctgt gcacttaata
     1141 caatatctgc cggcaaacct ttgcgggtaa atgcgtcatc agtgcgtgag cgccccgtaa
     1201 ttccctgtcg gtaagtaatt aacggccact tcgctattga ttccagcgtc aatggtgtaa
     1261 tttgcgttaa gggatgatcg agtggaacaa gcaaactatg gtgccaacaa aaccatggga
     1321 aggcgacgag ctgcgggtca ttactcaaac gctcgctggc gataccaata tcagcttcgc
     1381 cattttgcaa caacgtcgca atttcctgtg gcgaaccctg gattagctcg agccgaacct
     1441 ccgggaaaag ttcgcgaaaa gctttaatga cctctggcaa gctataacgt gcctgagtat
     1501 gcgtcgttgc aatagtgaga acaccagacg tatcgttggt aaacaggtct gcaagccgac
     1561 gaacattact ggcttcattc agaatacgtt ctgcaatgac cagtaatgct ttgcccggtt
     1621 cagtcatccc cagcagtcgc ttacctcgtc gaacaaatat ttcgatgcca aattcatcct
     1681 ccagttcccg aatatgacgg ctaacgcctg actgtgaggt aaaaagcata ttcgcaacct
     1741 ctgtcaggtt gtaatcctga cgtgcagcct cgcggattat ctttagttgt tggaaattca
     1801 cggtaaactc cgggcagttc agatatcccg ttattgttaa agtctaatgc ccggcataac
     1861 aaataataaa aacccgcatc ttattccatc ccgatataac gcttagctca ccaattgcca
     1921 ctgccttttt tccatcactg gagaactaat cactgacatt aacaactctt tcactgcctg
     1981 tgcctgtggt gacaagtttg ctctggcggg taaatttaag gacaaagaga gactcattga
     2041 aggagtggta atgcgtgaca tccacccatt tactgcgcca cataacgaac gcgcagccga
     2101 ttcaggtaat actgcaacgc ccatgccgct ggcaatcgct gcggtaagcg tggcaataga
     2161 ctcaatttca ccaatgactt ttgccgtgag ccgccgtagg gaaaaagcct catcaacacg
     2221 aagtctaaca gcgctgtaat cactggggag aaaaaggttc atttgcgcaa tagcattaac
     2281 atcaacgttt tgccccgggc aatcttgagt tcctaccagg aaaagatctt ctttcagcaa
     2341 agcctgactg gataccccag ccacagggga atgctcataa atcaccgcca tatcgagttg
     2401 gtgatttatc agtttttcgt taagcactgc accactattt tcatgaagat agataacgac
     2461 ctccgggaat tcagcgcgaa ccgcctgtaa taagggcatg gtgatggatg acgcagcggt
     2521 tcctggcgca aagccaatcg agacttgccc cgataatgac tgaccaacgt tatgcaccgc
     2581 cagttgggcc tgctcacact gacgtaaaat ggcccgtgca tgggtataga gaatttttcc
     2641 ggcgtctgtt ggcgtaacgc cccgttttgt gcgaatcaaa agctgttgat ttaactcacc
     2701 ttccagtatg gcaacctgtt ggctgagcgc tggttgtgcg atatgcaata cttcagcagc
     2761 ctgggtcagg ctaccaatat ctacaatttt tacgaagtat ttcaggcgtc tgaagttcat
     2821 gttgcctccg gtttttaaga atcggcccaa gtgccgccat tacttacaac cagtatgcaa
     2881 gatgcctgcc agttctattt tggcgttgat gcgcaagcta accaactgtc aaataagaga
     2941 ttatgataga ttcgtcattg gcccttttta tcagcagtcg cattcacctg ccctataaaa
     3001 ggagggtatg caccacgatg gttcattacc caataagatt gaaagctcac cactttgttg
     3061 aaattggcag caaacaaaca aaaaaaatgc atttcaccct ttgacatcac catgcactgc
     3121 cattaatatg cgccccgttc acacgattcc tctgtagttc agtcggtaga acggcggact
     3181 gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaaattcaa aaaagcctgc
     3241 tttcgagcag gctttttact ttctaattac caacgctctt aaaacatctg tcttgaacca
     3301 gaactaattt gcacaggcat tcccgatcga tgttgcaacg cagcatttgc gcgatttaca
     3361 tcaacttctt gcccgttgat aaacgcccgc aaagatgggg ttaccggcaa tggcactttt
     3421 cggtcagact catattctgc acgattgcgc gacagcggct catgaacttc cagccacttt
     3481 gaaccatccg gttccgttgt gtatttcact ggctgatcaa taatttgtac acgagtgcct
     3541 acaggaacat tatcaaacag atatttgata tcgtcattac gcagacgaat acagccctga
     3601 cttacacgga gcccaatacc aaaattggca ttggtaccgt ggatggcata caacctgcca
     3661 atataaatcg cgtacagccc catggggtta tcgggccccg caggaacaaa tgcgggcaaa
     3721 ctctcccctc gtttcgcata ttcgcgccgg atgttcggcg ttggcgtcca ggttggtgct
     3781 tcttgtttac gttcaacggt agtcacccag ttacgcgggg tttctcgccc agcctggccg
     3841 ataccaatag gaaagacttc cactgtatta ctgtctggcg gatagtaata aagacgcatt
     3901 tcagcgacgt taaccacaat ccctttacga acagtggcgg gcaaaatcag ttgctgcgga
     3961 atggtgagtt gtgagccagg cttcggcaaa aaaacatcag cgcccggatt cgcttccagc
     4021 atattactta acccttgccc gtattgtgcg gcaaaagtct ccagcggctg agtattgtga
     4081 tcaggaacag ttacagtaag cgactgcccc actaaacggc taccctctgg aggtaatgga
     4141 taagttaccg ccaggctatt ttggctggca aaaagcagag caaatgagca aagaatattt
     4201 acacgacgca tcatgtccct ttcctaagtc gcgaatacta tccgataagt atagctttta
     4261 tcagactttt cgtttttagc tgttcaaatc agaagtcgta ttccccggta gaacaatatt
     4321 actggcagca agttcgccca tgttgttgta tatcgcacaa gcagcttcga tgatggacat
     4381 cgccagagct gcaccactcc cctcacctaa acgcatatcc atattgagat aaggctccag
     4441 ccccaaatgc gagagcgcta tacgcgcgcc tttttctgcc gacaagtgag aaggaatgag
     4501 atacggtttg attgcaggag acatctggca ggctgcgagc gcagcagcat aagaaagaaa
     4561 tccatccagc aacacgggta aaccgcagga agcagcacct aacatcactc cagctattcc
     4621 gaccaaatca aatccaccca cttttgccag gacatcaaca ccatcctgag gatttggttg
     4681 attcaacgta atcgcccgac gcacaacatc aattttatta gccagtttat ctgtcggcag
     4741 gtttgcgcca atcccaacca cttcttcagg atcccggcca gtgattgtgc tgactattgc
     4801 cgctgccggt gtcgtgtttg ccatccccag ttcacctaca ccaaacagcg tgacaccgtt
     4861 tttcgccagc tcccgcgtat aacatatgac gtccaaaagc aacttttcag cctgacggcg
     4921 actcattgcc ggagctgaag caatattgcc gctacctcgt gcgacacgca tgttgataag
     4981 cccggggata ggctcagcag tatcaatacc aacatcaatt acgtggacgt tagcgcccgc
     5041 ttgtgctgcc agcacacaca cgccggttgt tccacgggtc atattttcag cctgtatggc
     5101 tgtcacttct tttggagaaa tagcgacccc ttcctcccag acgccgtgat cggcacacat
     5161 aaccagtacc gcttttttgc ccacatgcgg tatgccattc acccccggca ttcctgccag
     5221 ttgtatggca agcgcctcca gctttcctag gctaccaaca ggtttgaata acccgtcaac
     5281 atgccgttgt gcacgcgaca tagcggcaga attgatggct gggatcgtat tcagtaaatc
     5341 ggcaattatt tgcatgtcac gtttctttat agcagtgcca gcaggaagac cagttcacca
     5401 agctctatgg ctgctcccag cgtatcgccc gtttgcccgc ccagcgtacg tttgagaagc
     5461 tgaccgagga tgaaaatcgc caccatcgtc accaccatag cagccacacc atgcataccc
     5521 agcaataata cggcagcaaa aatagcggct aagccgagtg tgacgcaggt ttgtcgccca
     5581 tcaattttgc caatgaacac attgccaagc ccttcttccc gcgcataacg atgacgatac
     5641 atcaataatg cggcagtccc gcggctgacc gcacatgctg ccgccagcga tgcaaggatc
     5701 ggttcgccac gcagagccaa ctcgcttaac accagaatct ttgccagtac cacaaaaatc
     5761 aatgccagac cgccgtgggt gcctaaacgg ctatcacgca tgatttccaa catgcgatcg
     5821 cgactacgtg cagaaaatac gccatcacag gtatcagcga ggccgtccag atggaattcc
     5881 ccggtcatca gcgccagcac aagtacgcta aacagtgccg ccagtggtgc gccacaccat
     5941 gcctgcagca ccatgaagac cagcccgcta atcgcgccaa gcaataatcc aatcaaagga
     6001 aaagtaataa taccgcgaga ataatgttcg aaatccagtc cctgggacca gcgacgcggt
     6061 acaggcagac gcgtaataaa tgagagcatc gcccaaaata atttgctcat ttgattttta
     6121 ctccaatacc cgaaaccacc agccatactt catttgccgc agcggccaac tgctgattta
     6181 cccgcccggc aatatcacga aaatgtcgtg ccagacgact ctccggcaca atccccattc
     6241 ccacttcgtt agtcactaat acaacctttg cggggcaacg ttggcaggca gcaatcaacg
     6301 actgaatctc agcattaatc gcctgttcca tcgcctgata atcccattca tcagggtctt
     6361 tatcgccgcc ataatcaaac aacagattag tcaccattgt ggtaacgcat tccagcaaca
     6421 cagcctcatt cgggttaatg tctgcatgaa ttaattcatc aagatgttgc cagcgctcca
     6481 ctgtgcgcca gtgctccggg cggctttgcc gatgatgttc tatccgtgca gccatctcat
     6541 catcaaggat ttgcgaggta gcgatataca gaacctgtga aga
//
LOCUS       DAZXUK010000211         6569 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZXUK010000211 DAZXUK010000000
VERSION     DAZXUK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.211"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            192..1325
                     /gene="wzz(fepE)"
                     /locus_tag="ACH3L8_003278"
                     /pseudo
     CDS             192..1325
                     /gene="wzz(fepE)"
                     /locus_tag="ACH3L8_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
     gene            complement(1322..2137)
                     /gene="fepC"
                     /locus_tag="ACH3L8_003279"
     CDS             complement(1322..2137)
                     /gene="fepC"
                     /locus_tag="ACH3L8_003279"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFT4086263.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(2134..3126)
                     /gene="fepG"
                     /locus_tag="ACH3L8_003280"
     CDS             complement(2134..3126)
                     /gene="fepG"
                     /locus_tag="ACH3L8_003280"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFT4086264.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(3123..4127)
                     /gene="fepD"
                     /locus_tag="ACH3L8_003281"
     CDS             complement(3123..4127)
                     /gene="fepD"
                     /locus_tag="ACH3L8_003281"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFT4086265.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            4238..5470
                     /gene="entS"
                     /locus_tag="ACH3L8_003282"
     CDS             4238..5470
                     /gene="entS"
                     /locus_tag="ACH3L8_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFT4086266.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            complement(5474..6430)
                     /gene="fepB"
                     /locus_tag="ACH3L8_003283"
     CDS             complement(5474..6430)
                     /gene="fepB"
                     /locus_tag="ACH3L8_003283"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFT4086267.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
BASE COUNT         1528 a         1735 c         1799 g         1507 t
ORIGIN      
        1 attacagaaa ataattatgg gttttttatt tgtttgattt ataggtttga tgaatatttc
       61 tcttaaatag agtgaatgtt gcattcagtg gaaggaattt acaaacttca gcttcggata
      121 attctcacca actggtaaag tgagcgccgt ggagattttc ccctattgga ctcattttca
      181 ggttataccc catgtcatca ttgaatatta aacagggaag tgacgctcat ttttctgagt
      241 atcctctggc gtcgcccagt aataatgaaa ttgatttact taatctaatc gaagttttat
      301 ggcgggccaa aaaaacggtc atggcggtcg tttttgcatt tgcctgcgca ggtttactga
      361 tctctttcat cctgccgcaa aaatggacca gctcagccgt tattactccc gccgaagcca
      421 ttcagtggca ggacctggag aaaaccttta ccaaactccg cgttcttgat ctcgatgtta
      481 atatcgaccg tggcggtgca tttaacctgt ttatcaagaa gtttcagtct gttagcttgc
      541 tggaagagta cctgcgttca tcaccttatg tgatggacta attaaaagag gcgaaaatcg
      601 atgaactgga tttgcatcgc gctattgttg cattgagcga aaaaatgaaa gcggttgatg
      661 acaatgctag taagaaaaaa gatgaaccgt cactgtatac atcctggacg ctaagtttta
      721 ccgcgccaac cagtaaagag gcgcagaccg ttttgagcgg gtatatcgat tatatctctg
      781 cgttggtggt gaaagagtcg atagaaaacg tccgtaataa actggagatc aaaacccagt
      841 ttgaaaaaga aaaactggct caggatcgca ttaaaacgaa aaatcaactt gatgcaaaca
      901 ttcagcgcct caattattca ctcgacattg ccaacgcggc aggaattaaa aagcccgtat
      961 acagtaatgg tcaggccgtt aaagatgacc ccgatttctc tatttccctc ggcgcagacg
     1021 gtattgaacg caaactggaa attgaaaaag cggttactga cgttgcggaa ctgaacggtg
     1081 aattacgtaa tcggcagtat cttgtcgagc aattaacaaa agcaaatgtc aacgatgtga
     1141 attttacgcc gtttaaatat cagttaagcc cgtcattgcc agtgaaaaaa gacggtccgg
     1201 gtaaggcgat tattgtgatc ctttccgcgc tgatcggcgg gatggtggct tgtggtagcg
     1261 tgttattgcg ctatgcgatg gcatccagaa aacaggatgc catgatggca gaccacttag
     1321 tttaactatt tgcggttgaa ggtgccgttc gtccgagcgg caccacaagc ggcattccgg
     1381 ctaccggatc gtcaatgatc atgcatcgca gaccataaat gcgctcaatc agttcagcag
     1441 tgacaatctc cttcggcgct ccctgagcaa caattttccc ttcccgcaat gcaatcaaat
     1501 ggctggcgta acgacaggcc tgattaagat cgtgcagcac cgccgccagg gtatagcctt
     1561 tctcgcggtt cagttcgctt aacagttcca gtagatcaat ctgatgactg atatccagcc
     1621 aggtcgtggg ttcgtcgagc agcataattg ccgtttcctg ggccagcacc atcgcgatcc
     1681 acgctcgctg gcgttgtccg ccagaaaggg tatccacgct ttgatctgcc agatgagtta
     1741 ttcccgtggc ctgcatcgct ttcgttaccg cttcttcatc ctctttgcgc cagcgggtaa
     1801 acagcggttg atgcggataa cgtccacgcg ccaccagctc ctgcacggtg atatcgcccg
     1861 gcgtggtagc attttgcgcc aacagaccaa tccggcgtgc aacttcttta ctggcgtaat
     1921 gttgaatgtg ctcgccatcc agccagacat gcccgtgagc aggcgtcatc aggcggctta
     1981 aggtacgcag taacgtggat ttaccacagc catttggtcc gataattgcc gtgaagtgac
     2041 catcaggaat ttctacagtc agattttccg caacggtata tttgccatat cccagggtta
     2101 actgttcgcc gcgcaaacgg gctactgatt cggtcatttt ttgcgagact cctgaattaa
     2161 caagacgata aggtaaatac cgccgaggct gacggtaacg acgccaaccg gaagttgata
     2221 cggcataaac agttgttggg cgcagagaac ggccgccagc agtaacagcg ctccgcacag
     2281 cgccgcctgg gtcagccccc agcgagcggt gccgctaatg cgtcgggcaa tgtgcggcgc
     2341 gactaaagca ataaaggaaa tcggcccggc aagcgccgtt gccgcagcgg taagcaccac
     2401 tgcaaccagc atcattaaca gacgcgaacg ctcgacgctg acgcccagcg cgcaggcggt
     2461 atcatcgccc atctccagca agcgcattcg ccgcaccagc aaggtggcgg caatgagcat
     2521 caatataatg atgggtgcgg aaggcgaggt ttttgcccac gtcaggccgt tgagcgatcc
     2581 ggcattccac aaacctgctg ttagcgccgt ttctaaagac gctttcagca acagccaggt
     2641 attaaaggcc accagcatgg cgcgaacgcc gataccgata ataatcaacc gaaaggtgtc
     2701 gataccgtta cgccaggcga gcagccagac caacagcgaa gtgataatgc cgcccaccat
     2761 tgctgccagc gcgatagccg tcagatcctg accaaacagc accatcgcca ccagcacgcc
     2821 gctccacgcc ccggtgttaa agcccattac gtcagggctg ccgagcgggt tacgcatcag
     2881 cgactgaaaa atcgcgccgc tgacgcccag cgccgcgcca atcaacagcg ccatcagcac
     2941 gcgtggtaaa cgccattcgg tgaccaccat cgtcatactg cgcggcgcat cgcccatcaa
     3001 cgcggcgaat acctgcgaga tttccagcgt gacggcaccg ctgcgtaatc cccagatacc
     3061 tgcaaccaca caggcggaaa tcagcagcaa acaggtgatg agtaatcggc gagagacgta
     3121 aatcatgcac cacctcgcgt tttacgtcgc acgaggaaga tcagcaccgg tgcaccaata
     3181 aacgcactga ccacagaaac gcgcagttcg ccgggcacaa tcacccgccc gatgatatcg
     3241 gcaaacagca gcagggcagg ggtagcaagt agcgtgacgg gcagcgacca gcgatgatcg
     3301 gcacctacca gccagcgcgc catgtgcggc atcatcaggc cgataaaggc aatcgggcca
     3361 actaccgccg tcgcactacc acaaagcacg gtaatcgcca gcagaccaat caactgtgtg
     3421 cgcgccacgc gactgcccag cgccgtcgcg gtgtcactgc cgaggctcaa actgttcagc
     3481 gcgcgactca gtaatagcgc agttgctccg gcgatcagaa ccgggatcag caccactttt
     3541 aaggtatgta gattgcgaat atccagcgaa ccggcttgcc agaaacgcag ttgatcgtag
     3601 acgtcaggat taagcagggc gatgccgctg gtcagtcctt ccagcaccgc cgccagcgcc
     3661 acgcccgcca gggttaaacg caccggactt aactgccctc caccctgact gccggtaaag
     3721 gcaacaatca atgaggccac cagcgccccg gcgaaggcca tcgccagttg ttcctgtgcg
     3781 gatgagtaac caaacagcgc cgcacccagc acaatggcaa agctggctcc ggcgttcacg
     3841 ccaagcaagc cggggtcggc aagtgggttt cgggtgaggg tttgcattaa cgccccggca
     3901 aggccaagcg cgccgcctgc cagtaaaccg gcaagggtac gcggcagccg cgcgtcgagc
     3961 acgatggtgc agtcggcgct ctggcaggtg ccggagaagg cctccagcac tacggaagcg
     4021 gggagtgatt ttgccccaat gagcaggctt aatgccgtcg cgataatcag taataacagc
     4081 aatccgggca cggcaatggc gcgtgtcacg gcaacagaac cagatatacg aacttccatg
     4141 ataatgaaat taattatcgt tatcgatctt atttggatat gttaacatgt gcagcctaag
     4201 aataggtatt taaaatattt gatggcaagg cattgtaatg aataaacaat cctggctgct
     4261 taacctcagc ctgttgaaaa cgcacccggc gtttcgcgca gtattcctcg ctcgttttat
     4321 ctcaattgtg tctctgggtt tgctcggcgt cgcggtgccg gtgcagatcc agatgatgac
     4381 ccattctacc tggcaggtgg ggctttcggt gacgctgacc ggcggcgcga tgtttgttgg
     4441 cctgatggtt ggcggtgtgc tggcggatcg ctatgaacgc aaaaaagtga ttttgctggc
     4501 gcgcggcacc tgtggcattg gcttcattgg actgtgcctt aatgcactgc tgccggagcc
     4561 gtcattgctg gcaatctatt tacttggttt atgggatggt tttttcgcat cacttggtgt
     4621 tacggcgcta ctggcggcaa catctgcact ggtagggcgt gaaaacttaa tgcaggccgg
     4681 ggcgatcacc atgttgaccg tgcgcctggg gtcggtgatt tcgcccatga ttggcggttt
     4741 attgctggcg accggcggcg tagcctggaa ctacgggctg gcggcggcgg gcacgtttat
     4801 taccttgtta ccgttattaa gccttccgga gttgccaccg ccaccgcagc cgcttgagca
     4861 tccgttgaaa tcattactgg caggatttcg ttttctgctt gccagcccgc tgctgggtgg
     4921 tttattgacg atggcgagcg cggtgctggt actgtatccg gcgctggctg acaactggca
     4981 gatgtcagcg gcacagattg gttttctcta cgcggcgatc ccgctcggcg cggctattgg
     5041 tgcgttaacc agcgggaagc tggcacatag tgcgcgacca gggttattga tgctgctctc
     5101 cacgctggga tcgttcctcg ccattggtct gtttggcctg atgccgatgt ggattttagg
     5161 cgtggtttgt ctggcgctgt tcggctggct gagcgcggtt agttcgttgc tgcaatatac
     5221 aatgctgcaa acgcaaaccc cggaagcgat gttagggcgg attaacggtt tgtggacggc
     5281 gcagaacgtg acgggcgatg ccataggcgc ggcgctgctg ggtggtttgg gcgcgatgat
     5341 gacaccggtt gcttccgcaa gcgcgagcgg ttttggtttg ttgattatcg gcgtgttgtt
     5401 attgctggtg ctggtggagt tgcgacgttt tcgccagacg ccgccgcagg tgacagcgtc
     5461 cgacagttaa tgcttaaaac agcgccttaa gccgttccag cacttgcatg gcgctgtagt
     5521 aatccaggcg gaacgtctcg gttcccagcg cataaacctg cttgttttgt accgcaggca
     5581 ggtgcgcgag cagtggattg gcataaatcg catcggcatc tttctgatca ccagcgaaca
     5641 ggaatagcga ttcaccgttt aaccccgcag ccagattttc cccaccaagc tgaatgatgt
     5701 catggcgttt gccctggctt tggctggcgt ttaaccctgc gggcaatttc gccagcgtaa
     5761 agccgagttg ttccagcatc tgcccttgtg ctgattccgg cgtccagaga ttggcactgt
     5821 gtgcagcggc ggtatagaca atggcagtga ccggctgcgg cggtaatttg atttgctctt
     5881 tcgccgctgc cagttgctta tcaaactgtg caatccgctc tgccgcttgt ttctcatgcc
     5941 cggtaatttc gccaagctgc gttaacagcg actgccagct tttgtcgtcg taattgatga
     6001 ttaatgtcgg ggcgatggtg gaaagctgat catacagcgc cagcgccgaa tccccgccgg
     6061 ttgcgctaat taaaatcaga tccggcattt gcgcggcaac ggcttcggcg ctcggttcgc
     6121 cgatatagag ccgttgcagt ttgcgttctt tcgccacctt gctccactgg cgtaaaaagc
     6181 cctggtcatc tgcgacgcgg ttattcggcg tggtcgcgcc gctggcgatc accggagcat
     6241 caatcgccag cagtgatccg gtcagggtga cgctggtgga aacaatacgc tgcggctggc
     6301 tttccagggt atgagtgccc cggctgtcag taatctgacg cggccagtcg gcggcctgaa
     6361 ctacggctat tcctgaaagc aaaagtcctg ttaatagaag ggcgttgcgg tagagcgggg
     6421 cgagtctcac aagtcagctt cctgttatta ataaggttaa gggcgtaatg acaaattcga
     6481 caaagcgcac aatccgtccc tttggggaga gggggcaaaa cggcgcagga catcacattg
     6541 cgcttatgcg aattcatcaa taatgcttc
//
LOCUS       DAZXUK010000212         6564 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZXUK010000212 DAZXUK010000000
VERSION     DAZXUK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.212"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            157..504
                     /locus_tag="ACH3L8_003284"
                     /pseudo
     CDS             157..504
                     /locus_tag="ACH3L8_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            557..952
                     /locus_tag="ACH3L8_003285"
     CDS             557..952
                     /locus_tag="ACH3L8_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFT4086268.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="ACH3L8_003286"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="ACH3L8_003286"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFT4086269.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENAPMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="ACH3L8_003287"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="ACH3L8_003287"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFT4086270.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACH3L8_003288"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACH3L8_003288"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFT4086271.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="ACH3L8_003289"
     CDS             complement(5323..6423)
                     /locus_tag="ACH3L8_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFT4086272.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
BASE COUNT         1507 a         1717 c         1554 g         1786 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca ccgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aatt
//
LOCUS       DAZXUK010000213         6563 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZXUK010000213 DAZXUK010000000
VERSION     DAZXUK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.213"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="ACH3L8_003290"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="ACH3L8_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFT4086273.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="ACH3L8_003291"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="ACH3L8_003291"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFT4086274.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="ACH3L8_003292"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="ACH3L8_003292"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFT4086275.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="ACH3L8_003293"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="ACH3L8_003293"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFT4086276.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACH3L8_003294"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACH3L8_003294"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFT4086277.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACH3L8_003295"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACH3L8_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFT4086278.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..>6563)
                     /locus_tag="ACH3L8_003296"
     CDS             complement(6164..>6563)
                     /locus_tag="ACH3L8_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="DNA-(apurinic or apyrimidinic site) lyase; has
                     apurinic or apyrimidinic endonuclease activity and DNA
                     N-glycosylase activity; removed damaged DNA at cytosines,
                     thymines and guanines; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFT4086279.1"
                     /translation="GLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTAN
                     VVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLIL
                     HGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT         1716 a         1589 c         1656 g         1602 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgccatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg cca
//
LOCUS       DAZXUK010000214         6554 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZXUK010000214 DAZXUK010000000
VERSION     DAZXUK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.214"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..470)
                     /gene="pabA"
                     /locus_tag="ACH3L8_003297"
     CDS             complement(<1..470)
                     /gene="pabA"
                     /locus_tag="ACH3L8_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFT4086280.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIR"
     gene            complement(502..1104)
                     /locus_tag="ACH3L8_003298"
     CDS             complement(502..1104)
                     /locus_tag="ACH3L8_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFT4086281.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(1094..1261)
                     /locus_tag="ACH3L8_003299"
     CDS             complement(1094..1261)
                     /locus_tag="ACH3L8_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFT4086282.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(1366..1947)
                     /gene="ppiA"
                     /locus_tag="ACH3L8_003300"
                     /gene_synonym="rotA"
     CDS             complement(1366..1947)
                     /gene="ppiA"
                     /locus_tag="ACH3L8_003300"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFT4086283.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            2218..3399
                     /gene="tsgA"
                     /locus_tag="ACH3L8_003301"
     CDS             2218..3399
                     /gene="tsgA"
                     /locus_tag="ACH3L8_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFT4086284.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            3661..6204
                     /gene="nirB"
                     /locus_tag="ACH3L8_003302"
     CDS             3661..6204
                     /gene="nirB"
                     /locus_tag="ACH3L8_003302"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFT4086285.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            6201..6527
                     /gene="nirD"
                     /locus_tag="ACH3L8_003303"
     CDS             6201..6527
                     /gene="nirD"
                     /locus_tag="ACH3L8_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFT4086286.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
BASE COUNT         1566 a         1723 c         1695 g         1570 t
ORIGIN      
        1 tggcgaatcc ccataatctc gcgggtttcg ctccaggccg tcacgtcaaa gcacgctggt
       61 aatgagtcag gttccaccac cagcgaatgg tagcgtgtca cggtaagtgg atttgccagc
      121 ccccgaaata cgccctcacc gctatgtgta atcggcgagg ttttgccgtg catgaccttt
      181 gcggcgcgca caactttgcc gccaaatgcc tgcgccattg cctgatgacc gaggcagacg
      241 ccaagaatcg gcaagcgccc ggcatagtgg cgaataacgt caagggagat cccggcttca
      301 tctggcgtac aggggccagg tgagatgaca attttttgtg gtttaagggc gtcgatatcc
      361 gccagcgtca acgcatcgtt gcgcttaacc agcacatccg cccccagttc acaaaagtac
      421 tggtagaggt tccaggtaaa agaatcgtag ttatctataa gcaggatcat ggcggctccg
      481 gtacgaaaga accgcgctat tttactcaga ttccccggct tcgcttacca ctttgctaaa
      541 tatcatctgc agtgcggtga gatcccccat tgccccactc tgatttgcct gattccaggc
      601 ctctttttcg ataccctgcc agctcagttg atatccggca tgaatcgcca gttgctcgaa
      661 gaagatccgc tgtgccagac cacttcccac ccggaaggga tgcagcacgt tgatttcaca
      721 atagtaatgc gccagccgct cgacgaactt cgctttctcc aggccaacca gataaccttc
      781 ttcctccaga tcctgcatca gggcattgcc ctctttttcg atataagcaa agtggcagaa
      841 cggcgtatcg ccctgataaa tatcaacttc acgcagttgc cctgcccagt cgaaaatatc
      901 ctggtacagc tggcgatgga tagtgcgcaa atgcggtaaa ccgcgcacca gcggaccaag
      961 ctcaatggtc gcagcacgca gcgccgtcat ttcgtaagcg gcctgttcca gccgctgctg
     1021 ctggcggatg ttcagccggt tacgcatgat atcaaggcca ggataaagat acagatcgcg
     1081 cccttcgccg aatttatcgc tcatagtgcc tcctcagctc ttcaaggcgc gctaaagcct
     1141 ctgcggcagt aagagtgact aaaggcatct cgacgccttc aagacggcga ctggcctgga
     1201 aattacgatt acgctgaagc tcccagagac gggacttttg cttatcggtg agtttcttca
     1261 cttgatgcct ccctgaacgt gtccatttgc cacaagtata agcggcaaat ccgggttacg
     1321 ccgggagagc agaagcgcgg gcaagattgc ccgcgcgaga aatcattacg gcaggacttt
     1381 agcggaaagg ataactaccg gttttgacgg cacattctgg tacggaccaa catcatgagt
     1441 cggcacctgg gaaatcttat cggcaacgtc catgcctttc accactttac caaataccgc
     1501 gtaaccgaaa tcacgctgac catggtcaag gaaggcgtta tcggcaacgt tgataaagaa
     1561 ctggctggtg gcgctgtctt tgtcagcggt acgcgccatc gcgatggtgc cacgcgtgtt
     1621 gcgcaggccg ttatcggctt catttttgat tggcgggttt ggttttttct gctgcatctg
     1681 ctcggtgaaa ccgccgccct gaatcataaa gccaggaatg acgcggtgaa aggtagtgtt
     1741 gttataaaaa ccgctgttca cataatcgac aaagttttgc acagacactg gcgctttttg
     1801 tttatccagc tccagttcga tgttaccagc tgaggttgtc aacaatacgt gcgggtcccc
     1861 tttcgctgcc attgctgcgg gagaaagagc agaaagagca gaaagagcga aaacagcagc
     1921 catcgccgcc agggtcgatt tgaacatgag atttccttaa caaacagagc agaaaaaaag
     1981 caagtgaaga gattctaaat agccttcaag aacaaggcca tccctttacc taattttacg
     2041 tatctgaaat atatgtaacc tgacgatcac tttttattcc gtgatcaaaa tcacctctta
     2101 aaatgcaatt tagcaaccga ttgcaataaa acatttaaac agatcataaa atcacctaaa
     2161 atcgcccgct cgcggcatat cccgctatgc cattttattt ttgaacgcag gccaatcatg
     2221 actaacagca atcgcatcaa gctcacatgg attagctttc tctcctacgc actgactggt
     2281 gcgttggtta ttgtcaccgg gatggtgatg ggaaatatcg ccgattattt caatctgcct
     2341 gtttccagta tgagtaacac cttcaccttc ctcaacgccg gcattttaat ctctatcttc
     2401 ctcaacgcct ggctgatgga aatcgtcccg ttgaaaacgc agttacgttt tggctttctc
     2461 ctgatggtgc tggcggttgc cggtttgatg ttcagccaca gcctggcgct gttctcgacg
     2521 gcgatgttca ttctcggggt ggtcagcggc attactatgt cgattggtac attcctgata
     2581 acacaaatgt atgaagggcg tcagcgcggt tcccgcctgt tatttactga ctccttcttc
     2641 agtatggctg ggatgatttt cccaatgatc gccgcgtttc tactggcgcg cagcattgag
     2701 tggtactggg tttatgcctg catcgggctg gtgtatgtcg ctatttttat tctgaccttc
     2761 ggctgtgagt tcccggcact gggcaaacgt gcaccaaaaa cggatgctcc ggtagagaaa
     2821 gaaaagtggg ggatcggcgt actgtttctc tctgttgcgg cgctgtgcta catcctcggt
     2881 cagttaggtt ttatctcctg ggtgcctgag tatgccaaag gcctgggcat gagcctgaac
     2941 gacgcgggca cgctggtgag taacttctgg atgtcataca tggtcggcat gtgggcgttc
     3001 agctttattc ttcgcttctt tgatttgcaa cgcattctga ccgtactggc tggtctggct
     3061 gcgattctga tgtacgtctt taacaccgga acaccagtac atatggcgtg gtcaattctc
     3121 gctctgggct tcttctccag cgcgatctat accaccatca tcaccctggg ttcacagcag
     3181 accaaagtac cgtcgccaaa actggttaac tttgtcctga cctgcggaac catcggtact
     3241 atgttgacct ttgtggttac cggcacgatt gttgaacata gcggtccgca ggcggcactg
     3301 cttacagcaa acggtctgta cgctgtcgtc tttgtgatgt gcttcctgtt aggtttcgtc
     3361 agccgtcacc gtcagcataa caccctgacc tctcattaat tgctaatgcc ggacggcact
     3421 atcgtcgtcc ggccttttcc tctcttcccc cgctacgtgc atctatttct ataaacccgt
     3481 tcattttgtc cattttttgc acaaacatga aatgtcagac aattccgtga cttaagaaaa
     3541 tttatacaaa tcagcaatat accccttaag gagtatataa aggtgaattt gatttacatc
     3601 aataagcggg gttgctgaat cgttaaggta ggcggtaata gaaaagaaat cgaggcaaaa
     3661 atgagcaaag tcagactcgc aattatcggt aacggtatgg tcggccatcg ctttatcgaa
     3721 gatcttcttg ataaatctga tgcggccaac tttgatatta ccgttttctg tgaagaaccg
     3781 cgcatcgctt atgaccgcgt acacctctca tcttacttct ctcaccacac cgccgaagag
     3841 ctgtcgctgg tgcgcgaagg cttctacgag aaacacggca tcaaagttct ggtcggcgaa
     3901 cgcgctatca ccatcaaccg tcaggagaag gtgattcact ccagcgccgg acgtaccgtt
     3961 ttttatgaca agctgatcat ggcaaccggt tcctacccgt ggatcccacc aatcaaaggt
     4021 tctgatactc aggactgctt tgtctatcgc accattgaag acctcaacgc cattgaatcc
     4081 tgcgcccgtc gcagcaaacg cggtgccgtt gttggtggcg gcctgttagg tctggaagcc
     4141 gctggcgcgc tgaaaaactt aggtattgaa acccacgtta tcgaatttgc ccctatgctg
     4201 atggcggaac agcttgatca gatgggtggc gagcagctgc gtcgcaaaat cgaaagtatg
     4261 ggcgtgcgcg ttcacaccag caaaaacacc cttgagattg tgcaggaagg tgtcgaagcg
     4321 cgtaaaacca tgcgttttgc cgacggcagc gaactggaag tcgactttat cgtcttctct
     4381 accggtatcc gtccgcgcga taagctggca acccagtgtg gtctggacgt tgctccgcgt
     4441 ggggggattg tcattaatga ttcctgccag acttccgatc cggatatcta cgccatcggt
     4501 gaatgcgcaa gctggaacaa ccgtgtattt ggtctggtag caccaggcta caaaatggcg
     4561 caggtcgccg ttgaccatat tctgggtagc gaaaacgcct ttgaaggtgc tgaccttagc
     4621 gccaagctga aactgctggg cgtagacgta ggtggtattg gtgatgcgca cggtcgcacg
     4681 cctggcgcac gtagctatgt ttacctcgac gaaagtaaag agatctacaa acgcctgatt
     4741 gtcagcgaag acaacaaaac cctgctcggt gcggtactgg tgggcgatac cagcgactac
     4801 ggcaacctgc tgcaactggt gctgaacgct atcgagctgc cggaaaaccc ggattctctg
     4861 attctgccgg cgcactcggg tagcggtaag ccgtctatcg gtgttgataa actgccggac
     4921 agcgcgcaaa tctgctcctg cttcgacgtc actaaaggtg atctgattgc tgccatcaac
     4981 aaaggctgcc acacagttgc ggcgctgaaa gctgaaacca aagcgggtac tggctgcggt
     5041 ggctgtatcc cgctggtcac tcaggtactg aacgcggaac tggcgaaaca gggcatcgaa
     5101 gttaacaaca acctgtgcga acactttgct tattcgcgtc aggaactgtt ccatttgatc
     5161 cgcgttgaag gcattaaaac cttcgaagaa ctgctggcga aacacggcaa aggctacggt
     5221 tgtgaagttt gtaaaccaac cgtcggttcg ctgctggcct cctgctggaa cgaatacatt
     5281 ctgaagccgg aacatactcc gctgcaggat tctaacgaca acttcctcgc taacatccag
     5341 aaagacggca cctactcggt gatcccgcgt tctccgggcg gtgaaatcac cccggaaggg
     5401 ctgatggcag tgggtcgtat cgcgcgtgaa tttaatctct acaccaaaat caccggctcc
     5461 cagcgcctgg cgatgtttgg cgcacagaaa gacgatctgc cggaaatctg gcgtcagctg
     5521 attgaagctg gcttcgaaac cggtcatgcc tatgcgaaag cactgcgtat ggcgaaaacc
     5581 tgcgtgggta gcacctggtg ccgctacggt gttggcgata gcgtcggcct cggcgtggaa
     5641 ctggaaaacc gctacaaagg catccgtacg ccgcacaaaa tgaagttcgg tgtctccggc
     5701 tgtacccgtg aatgttcaga agctcagggt aaagacgtgg gtattatcgc cactgaaaaa
     5761 ggctggaacc tgtatgtttg cggtaacggc ggcatgaaac cgcgtcatgc ggatctgctg
     5821 gcagcggata tcgatcgcga aacgctgatc aaatatctcg accgcttcat gatgttctac
     5881 atccgtaccg ccgacaaact gacgcgtacc gcgccgtggt tagaaaacct cgaaggcggc
     5941 atcgattacc tgaaagcggt gatcattgac gacaaactgg ggctgaacgc tcatctggaa
     6001 gaagagatgg cgcgcctgcg tgaagcggta gtgtgtgagt ggactgaaac ggtcaataca
     6061 ccgtccgcgc agactcgctt caaacacttc atcaacagcg acaagcgtga cccgaacgtg
     6121 cagatggtgc cagagcgcga acagcaccgt ccggcaacgc cgtatgaacg tatcccggta
     6181 actctggtgg aggacaacgc atgagccagt ggaaagacat ctgcaaaatc gatgacatcc
     6241 tgcctgaaac cggcgtctgc gcactgttag gtgacaagca ggtagcgatt ttccgcccgt
     6301 atcacagcga ccaggtgttt gcgatcagca acattgatcc gttcttcgag tccagcgtgc
     6361 tatcacgcgg gctgattgcg gaacaccagg gcgagctgtg ggtcgccagc ccgctgaaaa
     6421 aacagcgttt tcgcttaagt gatggcttgt gcatggaaga cgaacagttt tccgtcaaac
     6481 attacgaagc acgagtgaaa gacggcgtgg tgcagctgcg cggttaatgt tttaacggga
     6541 ggcgcaatgc ctcc
//
LOCUS       DAZXUK010000215         6544 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZXUK010000215 DAZXUK010000000
VERSION     DAZXUK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.215"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..261
                     /locus_tag="ACH3L8_003304"
     CDS             <1..261
                     /locus_tag="ACH3L8_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086287.1"
                     /translation="SNDIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQ
                     DVPIKEMFWNLQGDLNLTCWFHYKDGQWRVITYIFWPPGARF"
     gene            286..2499
                     /locus_tag="ACH3L8_003305"
     CDS             286..2499
                     /locus_tag="ACH3L8_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFT4086288.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            2522..2967
                     /locus_tag="ACH3L8_003306"
                     /pseudo
     CDS             2522..2967
                     /locus_tag="ACH3L8_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            2989..6544
                     /locus_tag="ACH3L8_003307"
                     /pseudo
     CDS             2989..6544
                     /locus_tag="ACH3L8_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459289.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         1577 a         1639 c         1996 g         1332 t
ORIGIN      
        1 agtaatgaca tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca
       61 cgatataaat ttgttggtga gtattatatc ggcttgaatc aaacttatcg cataacagat
      121 cctgaatttc aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat
      181 ctgacatgtt ggtttcatta taaagacggg caatggagag tgattactta tattttttgg
      241 ccaccagggg ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct
      301 acgggtaata aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc
      361 gaaagtggcg gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag
      421 atggcagaac aggctgcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt
      481 aaagccgcag tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct
      541 ggtgcagttc aggatgcagc ggcgtcgctt gcagccggtg ctccgggggc gcataacgtc
      601 acagtaagtg gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg
      661 gaagggcttg ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac
      721 ctggggtacg tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg
      781 tgcgtcagta tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg
      841 gcgagggtgg tcggccatga gagccgttcg gtaacgtatg aattacgcat tgagccatgg
      901 ctgaagctgc tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat
      961 attctcgatg aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat
     1021 tatccacccc gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc
     1081 ctgatgcagg aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgctg
     1141 gtgctggtgg atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg
     1201 caccaggaag ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg
     1261 ctgcgtactg gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt
     1321 aaaagtaccg tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg
     1381 cctggtgatt acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg
     1441 cagcgcagcc caggcagccg ggcacatggg gccggtcata tccgtaccct catgaccggg
     1501 tacaccttta cgttgatgaa tcacccgacg gcagaaatta atcaggaata tctgctggta
     1561 cagaccactc tgtttttacg ggataatgcg cagcacagcg gtcaggatca gcattttacc
     1621 tatgtcacca cgtttgaact gcaccccacc agcgaagttt accgcccaca acgaaccctg
     1681 agcaaacccc acaccaaagg cccgcagagc gccatcgtca ccggcccggc ggggcaggaa
     1741 atctggacgg ataaatatgg ccgggtgaag gtgcagtttg gctgggaccg ttacggaaag
     1801 aatgacgaaa acagttcctg ctggatccgc gtcagctatc cgtgggcggg taaaggattc
     1861 ggcatgatcc agatcccgcg tattggccag gaagtactgg tggatttcaa aaacggcgat
     1921 ccggatttgc ccatcatcgt ggggcgcacc tataaccagg acaccatgcc gccgtggggg
     1981 ctgccgggca tggcgtcgca gagtgggata ttcagccact cattgcaggg agggccgact
     2041 aacggaaata tgctgcgctt cgatgataaa acgggtgcgg aagaggtgaa gttccatgcg
     2101 gaaaaagacc tcaataccac ggtaaaaaac aatgaaaccc acacggtcat ggtcgatcgc
     2161 ataaaaacca ttgtcaaaaa tgaaaccatc agcgtgggag aagaccgtaa tgccaccatc
     2221 acaaaaaatg atggtctttc cgtcaaactg gctcaaacca tcaatgtcgg aacgacatat
     2281 cgtctggatg ttggcgatca atttaccctt cgttgcggca atgcagcgct tgtattacac
     2341 aaggatggct cgattgagtt ttgcggaaaa caactgttac tgcacaccag cgatgttatg
     2401 caactcattg gcagtggcat tgatatgaat ccggatggcg gcacagccat gactgcggat
     2461 gatattgccc cgttacctac ccccgacgtt tctgaataaa aaatataaaa ggaatttttc
     2521 tatgaccagc ctttaccgta ttcaggaggg ctgctttgcc ctgcctgaaa cttttcttga
     2581 tcgcaccgtc aatatcttcg tcccatcggg caatgaaagg gcaactccca gtctgaatat
     2641 tttccgcgat acgctcaggc cagatgaaaa tctgaccacc tatattgacc gccagatagc
     2701 cttgatgaaa aaaaaaacct aggccagcac cgggtacttt cgcggggaac ggcactggcg
     2761 ggaactggcg aagctgccat gacaggtgaa cagattctcg caactcatcg ttctggaaaa
     2821 acagaagtgt accagcgtca ggccgggttt atcaccgggc cagcaaaggt actaatgttg
     2881 acgctgacca ctcagcgtcc ttttgatgat cacaccgacc agctatggac tgcatggttg
     2941 actagttttc agcctgctaa aagctaaaaa aaacagggag gttttgtcat gtatgaagcc
     3001 gcacgggtgg atgaccccat ctaccacacc agcgcccttg ccgggttttt gataggggcc
     3061 atcataggta tcgccattat tgccgtcgcc agctttgctt tttttacctg cggctttttg
     3121 gccgggctga ttttgagttt tctggctgat cagatagctt ccggagtcct gcaactgggt
     3181 gaggctattg ggcgctcgat acattccaca gtgggaaaaa ttctgaccgg ttctgataat
     3241 gtcagcacca acagtcgccc ggccgcccgg gccgtactga gtacggtgat atgcgaagac
     3301 cattccccgg aaatccgcat tgcccaaggt tccggcaaca tctacatcaa cagccagctg
     3361 cccgtaagga tgaccacact gagtgtgatg cagtgattga ggacggctcc cccaatgtct
     3421 ttctcggcgg cggtacgcag acggtactgg aaatcagccc ggaaatccct gactggctgc
     3481 gtcaggtggt ggatgtgctg tatgtggtcg ccggaatgct tggcgggctg gctggcgcct
     3541 ggcgtcaggc ggcaaagatg gggtcgaaat ttggtacaaa atgtgcggca aaatttattg
     3601 gtggcgaaat ggtcgggatg gccatctctg acacagtcat gggattgttc agcaatccgg
     3661 tagatgtcac cactgggcaa aaaattttgc tgccggagac ggattttacc ctgccaggac
     3721 gcctgcctgt cacctgttcg cgcttttacg ccagccacct ggaaactgaa ggattgctgg
     3781 gcagaggatg gcggctgaac tgggagataa acctgcgtga ggatgaaaca tatatcacct
     3841 tcatcggtgt acagggtcgg gaactgagtt atccgaaaga gatgctgata ccgggccacc
     3901 agatattcga cccggaagag cagttttacc tgagccgtct gcatgacgga tgctatgtgc
     3961 tgcattacac cgactgcagc tattacgtgt ttgatgaatt tgatgaccac ggcgtcgcgc
     4021 cgttactttt tatggagacg ccgtaccggc agcgtattgc tttcgggcgt gagaacggca
     4081 ggctggtgcg ggtggcctcc agcagcggac accatctgtt attgcaccgc acgatgacgc
     4141 aagcagggga acgcctgtcg catattgaac ttttgaaggg cgggcgtccc ggtaacctgg
     4201 tggaatatcg ttacgacgat aacgggcaac tgactggcgt ggtgaaccgg gctggtgtga
     4261 cggtgcgcca gtttgcctat gaaaatggcc tgatgacaga gcatcgcaac gcgacgggct
     4321 ttacctgtac ctaccactgg gaagagattg aaggtttccc gcgcgtggtg gaacacacga
     4381 ccagcgacgg cgaggattac cggttccact atgattttgc tggcgggcag acggtggtaa
     4441 ccggcaggcc ggagcagaag tggcagtggt ggtttgatga agagacgtat gtgactgcgc
     4501 accggacgcc gggcggcgga atgtaccgct tcacgtacaa cgaaaaccat tttccggtag
     4561 cggtggaact ccccggcgaa cgcagggtaa cgctggaata cgacaccctg agccgggtgg
     4621 tgaaagagac ggacccggca ggccgggtga cacagacgca gtggaacggc agttttgctg
     4681 aaatcacgcg ccgggcgctg gatgacgacc acgtatggaa ggcggattac aacgaacacg
     4741 gccaggtgat acgggagacg gacccggaag ggcgcgtcac gcggtacggg tatgatgatc
     4801 aggggctgcc ggagacggtc tgccaccccg gtaaacagca ggaccgttac acctggaacg
     4861 cgctggggtt actgagcagc caccgtcgca tcacgggcag tgtgcagtcc tggcagtata
     4921 cgcagcgcgg gatgctggcg cgccatacgg atgaagagaa acgggagacg cgctggcagt
     4981 acaccccgga agggctggtt gccagtctga gcaacggcaa cggggctcag taccggttca
     5041 gctacgacgg cgacggacgg ctgacgggag aacagcgccc ggacggcctg ataaggatgt
     5101 ttgcgctgaa tgcggacggg tttccggtca tcatcccgac gcagggcaca gagggcgggg
     5161 tgcgcaatga acagcaggag cgtgatgcgc tgggccgcct gctgcgcagt gacacgcagc
     5221 acagtacacg gaccttcagc tacaaccgtc ttgaccagat aacggaagtg acgctcaccc
     5281 ccacggaaga aggggaacgg ctgcaccaca tgcaggcgga cacggtgcgt tttgcgtatg
     5341 accgcagcgg gtggctgacg gcagagcaca gcgtgcacgg cagtatcaaa taccggcgcg
     5401 atgcgctggg caatcccacc gatatcacgt tgccggacgg gcagcatctc agtcatcttt
     5461 actacggcag cgggcatctg ctccagacgg cactggacgg tatcacggtc agtgaatatg
     5521 agcgtgacag cctgcaccgt caggtgatac gcacgcaggg gaaactggcg acgttcagcg
     5581 gctacaacgc cgacaaccgg ttaagctggc agcgcagcct gccaggtggc agccagaatc
     5641 cacagcaggc ggtactcccc cggcgcagaa cgacagcgtg acggcgcggg actatatctg
     5701 gaacgcagac ggtgaagtcg gcggcatcaa tgacaaactg cgcggttgcc tggtgttcag
     5761 ctatgaccgc agcggctggc tgaccagccg cacggggcag atgtatgacc atgaccatta
     5821 ttattacgac aaagcgggca acctgctgac ggatgagtat cagggagcgg tgatggataa
     5881 ccgcctgccg ggttacgggc gtgaccgcta ccgctataac gaatggggcg aactgacaga
     5941 gcgcagggac cagcagctgg agtggaacgc gcagggccag ttaacgcggg tcatcagcag
     6001 caacagcgag acgcggtatc agtacgatgc gctgggcagg cgcatcagca aggcgaccag
     6061 caatctccat accgaccggg gagaacgaag ccggacaacg tttgtgtggg aagggttccg
     6121 gctgttacag gagacaacgt ggcagggaaa gcggacatac ctgtacgatg cggaacagcc
     6181 gtacacgccg gtggctgcca tcacggggcg cggagagagc cagaaaatct ggtattacca
     6241 cacggacctg acgggcacgg tacacgaggt aaccgcgccg gacgggacgc tggtgtgggc
     6301 gggctaccag gcgggatttg gtgaaaaccg gggcgacatc agcaacagcg gggcgtactt
     6361 tgagcagccg cagcggctgc cggggcagta ctttgacgaa gaaacagggc tgcattacaa
     6421 tctgttcagg tattatgcgc cggagtgcgg gaggttcgta agccaggacc cgataggatt
     6481 aaacggtggg ttgaacttat atgcttatgc gcctaatccg ttaggttgga tcgatccgct
     6541 agga
//
LOCUS       DAZXUK010000216         6479 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZXUK010000216 DAZXUK010000000
VERSION     DAZXUK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.216"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..723
                     /gene="gshB"
                     /locus_tag="ACH3L8_003308"
     CDS             <1..723
                     /gene="gshB"
                     /locus_tag="ACH3L8_003308"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFT4086289.1"
                     /translation="LADLDVILMRKDPPFDTEFIYATYILERAEEKGTLIVNKPQSLR
                     DCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILKPLDGMGGASIFRVKEGDP
                     NLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEPVPYCLARIPQGGETRGNL
                     AAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIGDRLTEINVTSPTCIREIE
                     AEFPVSITGMLMDAIEARLQQQ"
     gene            832..1395
                     /locus_tag="ACH3L8_003309"
     CDS             832..1395
                     /locus_tag="ACH3L8_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFT4086290.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            1395..1811
                     /gene="ruvX"
                     /locus_tag="ACH3L8_003310"
     CDS             1395..1811
                     /gene="ruvX"
                     /locus_tag="ACH3L8_003310"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFT4086291.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(1987..2967)
                     /locus_tag="ACH3L8_003311"
     CDS             complement(1987..2967)
                     /locus_tag="ACH3L8_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFT4086292.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            2985..3689
                     /gene="yggS"
                     /locus_tag="ACH3L8_003312"
     CDS             2985..3689
                     /gene="yggS"
                     /locus_tag="ACH3L8_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFT4086293.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            3707..4273
                     /gene="yggT"
                     /locus_tag="ACH3L8_003313"
     CDS             3707..4273
                     /gene="yggT"
                     /locus_tag="ACH3L8_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFT4086294.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            4270..4560
                     /gene="yggU"
                     /locus_tag="ACH3L8_003314"
     CDS             4270..4560
                     /gene="yggU"
                     /locus_tag="ACH3L8_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFT4086295.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            4568..5161
                     /locus_tag="ACH3L8_003315"
     CDS             4568..5161
                     /locus_tag="ACH3L8_003315"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFT4086296.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            5154..6290
                     /gene="hemW"
                     /locus_tag="ACH3L8_003316"
     CDS             5154..6290
                     /gene="hemW"
                     /locus_tag="ACH3L8_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFT4086297.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         1541 a         1697 c         1683 g         1558 t
ORIGIN      
        1 ctggccgatc tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc
       61 tacgcgacct atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg
      121 cagagcctgc gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca
      181 gaaacgctgg ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac
      241 atcattctta agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc
      301 gatccaaacc tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg
      361 gcgcaaaatt acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc
      421 gagccggtac cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg
      481 gctgccggtg gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt
      541 cagatcgggc cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc
      601 gaccgtctga ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag
      661 tttccggtgt cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag
      721 taacccacct tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca
      781 cgcatactgg gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta
      841 cagcatcact ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg
      901 gtctacattt gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg
      961 gaaaatctca aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat
     1021 gaatcaatcc gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg
     1081 tttattttgc atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg
     1141 gtgatgacca cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac
     1201 gtattggtgg ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc
     1261 gataacgcgt ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc
     1321 gaccgctggc gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg
     1381 gcaggacacg cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg
     1441 gcgtagcggt cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc
     1501 aggacggtac gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg
     1561 aaatcatcgt cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag
     1621 cgcgcaaatt tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg
     1681 agcgtcttag cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc
     1741 tcaacaaagg caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc
     1801 agggatatta aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg
     1861 cggcgtgaac gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt
     1921 tagcgtcgca tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg
     1981 gcaacgtcac aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat
     2041 ccccaccttc tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat
     2101 caaattcccc accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg
     2161 tttatccact tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg
     2221 tacgggatct ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc
     2281 accacgtgta tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag
     2341 acggattgtc tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa
     2401 tgcagcccgc aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat
     2461 caaacatcgc tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc
     2521 atcggcatgt tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc
     2581 cgtagccccc gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg
     2641 tggtgcacca agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga
     2701 aataccttgc cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac
     2761 ggcaaaatcc agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc
     2821 ccgcagtagc tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat
     2881 gcgccatcgt gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact
     2941 aagggccaca atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc
     3001 ataacctggc acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc
     3061 cagaagaaat tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag
     3121 ccattgatgc cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa
     3181 ttcgccactt tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc
     3241 agtctaataa aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt
     3301 tgcgcattgc tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac
     3361 tgattcaaat taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg
     3421 acgagctggc agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa
     3481 tccctgcgct tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag
     3541 catttgccgg actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg
     3601 acgatatgga agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt
     3661 ttggtgcgcg tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac
     3721 tttcctgctt tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat
     3781 gcagtgtgct cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca
     3841 gccaattatc gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc
     3901 gctgctggtt gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac
     3961 cttcctgcca atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct
     4021 gattttctgg gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc
     4081 gattgaatac gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct
     4141 accggcaatg ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat
     4201 caatatgggt gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg
     4261 gatggcgtta tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt
     4321 cagccgaaag ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc
     4381 attaccgcgc cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag
     4441 caattccggg ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa
     4501 caaattaaaa tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag
     4561 gtatcctatg caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc
     4621 gtcgctgctt agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc
     4681 cgctgaagaa accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc
     4741 aaaagtgacc ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg
     4801 tggcgcgccg ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa
     4861 tctgcaaaaa ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt
     4921 ccactgcgtg ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg
     4981 cagctggccg ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc
     5041 aatcttcttc gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag
     5101 cgccatttcc caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta
     5161 aattaccgcc gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact
     5221 gcgatttcaa ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc
     5281 tgctttgcga tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct
     5341 ttattggcgg tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg
     5401 gcgtgcgtgc gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag
     5461 gcacggtaga agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta
     5521 ttggtgtgca gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc
     5581 aagaagcgaa acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg
     5641 atttgatgca tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg
     5701 ccattgaact gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc
     5761 tgtttggctc gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac
     5821 aggggcatca gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca
     5881 aacccggtta tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta
     5941 ttggctgcgg cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta
     6001 aaacgcgtca tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg
     6061 aagccgcaga taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc
     6121 cgcgcgtgga gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag
     6181 acgaggcgat tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac
     6241 atggtaaatt attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc
     6301 gcctgcgggc gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat
     6361 atttcttact gaataaatat cgacgacatt tattatatat atttagtgaa tatttactaa
     6421 tcattcattg attataaata tgtacaacct ggcgcacaaa tttaatattt aatttctga
//
LOCUS       DAZXUK010000217         6321 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZXUK010000217 DAZXUK010000000
VERSION     DAZXUK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.217"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..378
                     /locus_tag="ACH3L8_003317"
     CDS             <1..378
                     /locus_tag="ACH3L8_003317"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086298.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACH3L8_003318"
     CDS             complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACH3L8_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFT4086299.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACH3L8_003319"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACH3L8_003319"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFT4086300.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="ACH3L8_003320"
     CDS             complement(1787..2476)
                     /locus_tag="ACH3L8_003320"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFT4086301.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACH3L8_003321"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACH3L8_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFT4086302.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACH3L8_003322"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACH3L8_003322"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFT4086303.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACH3L8_003323"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACH3L8_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFT4086304.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1609 a         1709 c         1565 g         1438 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc t
//
LOCUS       DAZXUK010000218         6267 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZXUK010000218 DAZXUK010000000
VERSION     DAZXUK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.218"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            44..397
                     /locus_tag="ACH3L8_003324"
     CDS             44..397
                     /locus_tag="ACH3L8_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFT4086305.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(434..2512)
                     /gene="flhA"
                     /locus_tag="ACH3L8_003325"
     CDS             complement(434..2512)
                     /gene="flhA"
                     /locus_tag="ACH3L8_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFT4086306.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="ACH3L8_003326"
     CDS             complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="ACH3L8_003326"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFT4086307.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="ACH3L8_003327"
     CDS             complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="ACH3L8_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFT4086308.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="ACH3L8_003328"
     CDS             complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="ACH3L8_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFT4086309.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="ACH3L8_003329"
     CDS             complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="ACH3L8_003329"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFT4086310.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="ACH3L8_003330"
     CDS             complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="ACH3L8_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFT4086311.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
BASE COUNT         1420 a         1725 c         1662 g         1460 t
ORIGIN      
        1 attaagatgt aatataaatg catcttaatg aggtggaggc agcatgtccg gcaagcgtat
       61 ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca
      121 ggcgtcagcg gagccggagc attacgaggc gttgtttgtt tacgcgcaaa agcggctgga
      181 taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca
      241 gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg
      301 gcgtcatccg atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccagaact
      361 gccggaaaaa tatcgtccga aaaaacctcg tgaatagtat gccggacagg cagatctgtc
      421 cggcaatttg cagttaaatc aatgccgatt tatcaattcc cagccgcttc atccgtgaca
      481 gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc
      541 tgttggtttc tttcagcacg cgcacaatca actgatattc atcttcgccc tcctgggcga
      601 caaccgttgc ggcaggcggc gtttcaggct ccggtaaagc aatatctggc aatgacagct
      661 gcagcacgtt gccgcgtgtt agcaatactg cgcgctcaat gacgttttcc agttcgcgta
      721 cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt ctcggcagga atgctgtcga
      781 tattgcgccc cagacgacgg gcaattttga aggtaaaggc tttcgccagc agcggaatat
      841 cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa tacgttcagg cggtaataga
      901 gatcgctacg gaactctcgg tcggcgacca tttttttcag atcgcggtta gtcgcggcaa
      961 ttaaacgtac gtccgtctga atgattttgt tgctgccgag acgttcaaac tcctgttcct
     1021 gcaatacacg cagcaacttc ggctgtaact ccagtggcat atcgcccact tcgtcgagga
     1081 acagagagct tttatccgcc agttcaaaac gaccaatgcg ctgggcgttg gcaccggtaa
     1141 aagccccacg ctcatgacca aacagatcgc tttccagcaa tccggcaggc atcgccgcgc
     1201 agttcatttt gaccatgcgg cgattattac gcccactgag attatggatc gcacgggcaa
     1261 tcagctcttt acccgagcca gtttcaccga ggatcagcac ggtactgtca ctttgcgcca
     1321 ccatttcaac ttgtttaagc acgctgtaca tggcttcgct gcggccaata atctcgccaa
     1381 attcactatc aacattgttg agctgctcgg tcagggcgag gttttcatca accagccgtt
     1441 ctttcagacg atggatttcc tgataggcga gggcgttatc gacagcgatt gccacacgtt
     1501 cggcaatctg gcgcagcaaa ttcagattgg tagtggtaaa cactttctct tcgcattgcg
     1561 ccagtttcag cacgcccagc atggtgtcgc cagacatcag cggtaacagg cacaaggttt
     1621 gaatctggtt gccccaggtg tcgaacaaca tgcgttcata gggggctaaa tcgtcccgct
     1681 cgtagagatt gatcagcagc atctctttac ttttgaacac gcgttcggtg agggttccgg
     1741 cttcatcgac ttcgctctgt tcgtgggcgg gatgttgttt atcaagatag tgagtggagt
     1801 agatgttgag tttgttttta cggtggctgc gtaagacaat gctgatatcg tcaatgtcga
     1861 aatagtaatg gatttctttg gcgacttcgc tgaccagttc gtccatatcc aggcgggaaa
     1921 gcaccgcgtt ggtaatggca accaggatgc ggaagttatc gcgctcccgg cataacaact
     1981 catagtcgac attgttgacg acgcggctct ggatttgttc ggtgacgaca gaaacgatct
     2041 gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca gggacgctcg tcataacgaa
     2101 taaattcaca gccaccaaaa atatgccctc ccgccgccag tggcatcagg caatagtgac
     2161 caaatttggg gtatagccca cctgcggcca gctgcggcca ggtttcacaa aattcttcgt
     2221 aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg taccggaccg tgtgccagaa
     2281 cagtttcgtc ttcatattta atgggggtgt ctttttcacg cgacgcgtaa taagacgcac
     2341 gttgagtctg cgcttgccac aacacaatag ccgcgttgtc ggcgagcgca gaacgcttta
     2401 ccagttgcga aagagcctca cacagcgagg ccagatcggg ctgctgcaat agtgtccgag
     2461 tgatgtcgaa caacccttgc tgtccgagat cactcatcgg tgtatatgac attttgttct
     2521 tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac taactatagg agatttagaa
     2581 ttttattagc atatacgcgg aagcggttcg gcgtgtggta aatcgagggt tcgtttcacg
     2641 ccatacagac cggcaagacg aacaccttta cgttccacca cttcaccaat cagcgccgcg
     2701 tctttcccca gtggatggga atgtaacgct gccagcactt gctctgccgc gttgcgttca
     2761 acggcgatca ccagtttgcc ttcgttggca aagttcaggg cgtccagtcc cagcaattcg
     2821 caaacgccgc gcacggcagg tttaaccggc agcgctgatt cagaaatttc aataccacaa
     2881 ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac caccacgggt ggcatcacgc
     2941 agcgctttca cgccgggaat gtcacgcagc gtctgaataa gcggcgtcag caccgcgcag
     3001 tcgctgacca gttcgccatc cagccccagc tgctcacgca ggttaaggat agtcgccccg
     3061 tggtcgccga gtgtaccgct aaccagcaat acatcgcctg cggttagcgt ctgtgcgccc
     3121 cagtgaatat tcgccggaat tgcgcccatg ccagcggtgt tgataaacag tttatctgcc
     3181 gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg caatgcctgc cgtgcgggcg
     3241 gtttctgcca tgctggtcac tacggctttc agtgtctcca tcggcaatcc ttcttcgagg
     3301 ataaagccac aggagagata gcgcggaata gcgccactgc ccgcaacgtc attcgcggtg
     3361 ccgcaaatcg ccagcttgcc gatattaccg ccaggaaaga acagcgggtc aatgacgtaa
     3421 ctgtcggtgg agaacgccag acggtcgcct tccgctacca gctgcgccag atcaagacgt
     3481 gcctgatctt cctgctctgc cagccacggg ttggcaaagg cttccataaa caggctgttg
     3541 attaattgct gcatcgcctg gccgccgctg ccgtgggcga gttggatatt attcacgctt
     3601 cactctcctg ctgacgatac tgataccacg cggcgcacgc tccttcggag gagaccatca
     3661 gtgcaccaaa tgcggtttga ggattacagg tgttaccaaa cagcgggcat tgatgcggct
     3721 tacatttgcc cgttaatacc tcaccacaac gcgcgcgcgg gtcatcgcag acctgctgcg
     3781 gtgccgggcg gaaatgtgct tcggcatcga atcgttgata atccggcgtc aggtgcacgc
     3841 cagaagattc aatcacgcct aagccgcgcc attcgctgtc gccgttgaca cagaacacat
     3901 cggcaatcgc ctgttgcgcc agtaggttac cggcatccgg taccacccga cgatactgat
     3961 tctctacctt gctgtgggcc gctattttct gctccaccag catgaccacg ccttgcagta
     4021 gatcaagggg ttcgaaacca gcgaccacca gcggacgatg aaaatcgctg gcgataaaat
     4081 tataggcatc agtgccgata accatactaa cgtggcccgg cgcgaggaac gcgtcgatac
     4141 cgttatccgg ctgttccagc aaactgcgca gcgtcgggat aagcgtaatg tgctggcaga
     4201 agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg cagagtgatg gcggtggtcg
     4261 gcatggtggt ttcaaaacct aagccgaaga acaccacttt gcgggttgga ttctcctgcg
     4321 ccagtttcaa cgcatccatc ggcgagtaaa cgatgcgcac atcggcaccg cgtgcttttg
     4381 cctgcaacag cgatccctgt ttccctggta cgcgcatggc gtcgccaaag gtacagaaga
     4441 tgacttccgg atggctggca atctccacgc aggtgtcgat tctacccatc ggcagtacgc
     4501 acaccgggca acccggaccg tggataaact caacgttttc aggcagtaac tggtcgaggc
     4561 cgaatttaaa aatggcgtgg gtatgaccgc cacacacttc cataatccgc agagggcgtt
     4621 cggcggtgta agagagatgt gaagcacgtt cgcgcagatg ctcaattaac tgcatcacct
     4681 gttccggcgc gcgatattca tcaacaaaac gcattatttt tcctcgccat acaacagcgc
     4741 gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg tcgagagtgt cgcgtgcttc
     4801 ggcttcatta attacgctca tggcaaagcc aacgtgaacc agtacccact ggcccacgcg
     4861 cggctgaccg ttttcatcgc agctgccgac taacgttaaa tcgacatcgc gctgaatgcc
     4921 gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg atctggccgg gaacgcctat
     4981 gcacatcgct gtgtctccag ccagttcagc cactggtcca tcccttcgcc gctggtggcg
     5041 gaaataagga tgatttcaat ttctggattg acttcgcggg cgcaggcgat gcacttctca
     5101 acgtcaaagt tgagatacgg caacaggtca actttgttga gcagcatcag cgaggcggcg
     5161 gcaaacatat gcggatattt cagcggtttg tcttcacctt cggtaacgga aagcaccgct
     5221 actttgtgtt tttcaccgag atcgaagctg gccgggcata cgaggttgcc aacgttttcg
     5281 ataaacagaa taccgttatc gtccagtggc agacgcggtg cggcgtcggc aatcatctgt
     5341 gcgtcaagat ggcagccttt accggtgttc acctgaatcg ctggtgtacc ggtagcgcga
     5401 atgcgtgcgg catcgttcac ggtttgctgg tcgccttcaa taaccgcgca cggaacgctg
     5461 tctttcaggc gcattaaggt ttccgtcagc agggtggttt taccggaacc agggctggaa
     5521 accaggttga gcaccagttg cttgcgggca gcaaagcgcg cgcggttgcg ttcagccaga
     5581 cggttgtttt tgtccagcac gtcaatttcg acttccagca tccgacgctg gctcatgccc
     5641 ggtgtgtgag tgcccgcttc gccatgaccg taatgcaggt cgccttcttc agtctggctg
     5701 ggggtaaatt caggcgcttt aatgccggtg attttcatct gcgggcgtgc cgccggggca
     5761 aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat caccctcgat atacaggttg
     5821 ccttcaccgc aaccgcatgt tgtacacatc gctcactcct ggtctatttc tatccgccga
     5881 atctgtaaac cgtcgtctgc cacaatctgt agcatgtcac catgacactg tggacagcgg
     5941 cggacgcgct gggtcagtag cgtcacatac tgttggcatg tttcacacca gcattcggcc
     6001 tcttgttctt cgaggtgcag tttacaacct tccgccacgc tgccgcggca aaccagatca
     6061 aaacaaaagg caagagagct ggtttcgaca caagaaaatg cgccaatttt gagccagacc
     6121 ccagttacgc gttttgcgcc gtgttttgcg gcctgctgtt cgatcaattc cagtgcccgt
     6181 tggcagaggg ttatttcgtg catatcgcct cccattaact attgccagct acaagcaata
     6241 attgcgccag tgttgattat ccctgcg
//
LOCUS       DAZXUK010000219         6257 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZXUK010000219 DAZXUK010000000
VERSION     DAZXUK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.219"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACH3L8_003331"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACH3L8_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFT4086312.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACH3L8_003332"
     CDS             2219..2767
                     /locus_tag="ACH3L8_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFT4086313.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACH3L8_003333"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACH3L8_003333"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFT4086314.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACH3L8_003334"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACH3L8_003334"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFT4086315.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACH3L8_003335"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACH3L8_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFT4086316.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1566 a         1540 c         1594 g         1557 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaa
//
LOCUS       DAZXUK010000220         6254 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZXUK010000220 DAZXUK010000000
VERSION     DAZXUK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.220"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACH3L8_003336"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACH3L8_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFT4086317.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="ACH3L8_003337"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="ACH3L8_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFT4086318.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACH3L8_003338"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACH3L8_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="ACH3L8_003339"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="ACH3L8_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1659 a         1458 c         1476 g         1661 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atga
//
LOCUS       DAZXUK010000221         6107 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZXUK010000221 DAZXUK010000000
VERSION     DAZXUK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.221"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            205..975
                     /locus_tag="ACH3L8_003340"
     CDS             205..975
                     /locus_tag="ACH3L8_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFT4086319.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            966..1439
                     /locus_tag="ACH3L8_003341"
     CDS             966..1439
                     /locus_tag="ACH3L8_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFT4086320.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1546..2085
                     /gene="dnaT"
                     /locus_tag="ACH3L8_003342"
     CDS             1546..2085
                     /gene="dnaT"
                     /locus_tag="ACH3L8_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFT4086321.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2088..2825
                     /gene="dnaC"
                     /locus_tag="ACH3L8_003343"
     CDS             2088..2825
                     /gene="dnaC"
                     /locus_tag="ACH3L8_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFT4086322.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2874..3368
                     /gene="yjjA"
                     /locus_tag="ACH3L8_003344"
     CDS             2874..3368
                     /gene="yjjA"
                     /locus_tag="ACH3L8_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFT4086323.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3608..5899
                     /gene="opgB"
                     /locus_tag="ACH3L8_003345"
     CDS             3608..5899
                     /gene="opgB"
                     /locus_tag="ACH3L8_003345"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFT4086324.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6037..>6107)
                     /locus_tag="ACH3L8_003346"
     CDS             complement(6037..>6107)
                     /locus_tag="ACH3L8_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFT4086325.1"
                     /translation="LAETYERDVINNRELIKGVITF"
BASE COUNT         1521 a         1571 c         1548 g         1467 t
ORIGIN      
        1 aaattcatta tagtgataat attcatgtga tagtttgttt gcggcgagag ataattcgct
       61 ttttatcacc gagcttgtcc caataaccag gattaatcag cgcaattttg atcttaacct
      121 accgcaaaac aaccgtgctt catgatacaa atggattctc attatctgaa caatacgcag
      181 cacgtctatg acaaagggcg agttatgcaa actgagcaac agcgagccgt aacgcggctt
      241 tgtatccagt gtggattatt tcttttgcaa catggtgcgg aaagcgcgtt ggttgatgag
      301 ctttcctcac gactgggtcg ggcgctggga atggacagcg tcgaaagttc tatctcttcg
      361 aacgccatag tgctgacaac tattaaagat gggcaatgcc tgacatcgac acgtaaaaat
      421 cacgatcgcg gcattaatat gcatgtggtg actgaagtcc agcacattgt gattcttgcg
      481 gagcatcatc tgctggatta caaaggcgta gagaaacgat ttagccaaat tcaaccatta
      541 cgttacccaa gatggctggt agccttaatg gttggccttt cttgcgcctg tttctgtaaa
      601 ctcaataacg gtggctggga tggtgccgtc atcaccttct ttgccagtac gaccgcgatg
      661 tatatccgcc agctgctggc acaacgtcat cttcatccac agatcaactt ttgccttacc
      721 gctttcgccg ccaccaccat ttccggattg cttttgcaac tccccacttt cagcaatacc
      781 cccaccattg cgatggccgc cagcgttctg ctgctagtgc cgggctttcc gttgattaat
      841 gccgtcgccg atatgtttaa aggccacatc aataccggac tggcacgctg ggcgatcgcc
      901 agtctgctga cactggctac ctgcgtcggc gtagtgatgg cactgacgat ttgggggcta
      961 cgcggatggg tgtgatcgaa tttctgttag cgttggcgca ggatatgatc ctcgccgcca
     1021 ttcctgcggt cggctttgcg atggtgttca acgttcccgt gcgggcgtta cgctggtgtg
     1081 cgctgcttgg ctcgataggt catggttccc gaatgatctt gatgaccagc gggttgaata
     1141 ttgagtggtc aacctttatg gcttctatgc tggtcggtac cattggtatt caatggtcgc
     1201 gctggtatct ggcgcatccg aaagtgttta ccgtggcggc cgttatccct atgttcccgg
     1261 gcatatcggc ttataccgca atgatttcgt cggtaaaaat cagccagtta ggttacagcg
     1321 aaccgttgat gattaccctg ttaaccaact ttcttacagc ttcatcgatt gttggtgcgt
     1381 tatccatcgg tctttccatt cctggattat ggttgtaccg caagcgccct cgcgtataaa
     1441 attgcctcgc ttaagcacac ggatgagaga cagcctcctc tcctccgtgt gttactataa
     1501 aagttatctc ccttctcgtt catcgttcca tatttgagaa acagtatgtc ttccagagtt
     1561 ttgaccccgg acgtcgttgg tattgacgcc ctggtacacg atcaccaaac cgttctggca
     1621 aaagctgaag gcggtgtggt tgccgtattt gctaacaatg ccccggcgtt ttatgccgtc
     1681 acgcctgcac gcctggctga actgctggcg ctggaagaaa agctagcgcg tccgggaagc
     1741 gatgtcgctc tggacgatca actctatcag gaaccgcaag ccgctcccgt tgctgtaccc
     1801 atggggaaat tcgccatgta tccggactgg caacccgatg ccgattttat ccgcctggcg
     1861 gcgctatggg gcgtggcgct aagagagccg gtgaccaccg aagaactggc ctcattcatt
     1921 gcctactggc aggcggaagg taaagtcttt caccatgtgc agtggcaaca aaaactggcg
     1981 cgcagcctgc aaatcggtcg tgccagcaac ggcggactgc cgaaacgaga tgtgaatacg
     2041 gtcagtgaac ctgacagcca aattccacca ggattcagag ggtaacgatg aaaaacgttg
     2101 gcgacctgat gcaacgcctg caaaaaatga tgcctgccca tatcaaacct gcattcaaaa
     2161 cgggtgaaga gctactggcc tggcagaaag aacaaggggc gatccgctcc gccgctctcg
     2221 aacgtgaaaa tcgggcgatg aaaatgcagc gcacctttaa ccgctccggt attcgtccac
     2281 tgcatcagaa ctgctccttt gagaactatc gcgttgagtg tgaagggcag atgaatgcgt
     2341 taagcaaagc gcgccagtat gtcgaagagt ttgacggcaa catcgccagc ttcatctttt
     2401 ctggtaagcc aggaaccggc aaaaaccatc tggcggcggc aatctgcaac gagctgctgc
     2461 tacgcggtaa atccgtattg atcatcaccg tggccgatat tatgtcggcg atgaaagata
     2521 ccttcaggaa tagcggtacc agcgaagaac aactgcttaa cgatctgagc aacgttgatc
     2581 tgctggtgat cgatgagatc ggcgtgcaga ccgaatcgaa atacgaaaaa gtgatcatca
     2641 accagatcgt cgatcgccgc tcttcttcca aacgcccaac cgggatgctg accaacagta
     2701 atatggaaga gatgaccaag ttgctgggcg aacgcgtgat ggaccgtatg cgcctgggta
     2761 acagtttatg ggtgatcttc aactgggata gctaccgtag ccgggtaacg ggtaaagagt
     2821 attaatcccc gtttctggcg gtatactgcg acacaattcc accttgagta atgatgatga
     2881 aaactattaa acatcttctg tgctgtgcca ttgccgccag cgcattaatt tccaccgggg
     2941 tgcatgctgc gtcctggaaa gatgcgctct ccagcgccgc cagcgaactt ggcaaccaaa
     3001 acagcacgac acaggaaggc ggttggtcgc tcgcgtcatt aactaacttg cttagcagcg
     3061 gaaaccaagc cttaagcgca gataacatga acaatgccgc aggcattctg caatactgcg
     3121 cgaagcaaaa gctggcttcg gtaaccgatg ccgaaaacat caagaaccag gtgctggaaa
     3181 agctgggcct gaacagtgaa gagcaaaaag aagacaccaa ctatctggac ggtattcagg
     3241 gtttgctgaa aacaaaagat ggtcagcaac tcaatctgga taacatcgga acgactccgc
     3301 tggcagaaaa ggtgaaaacc aaagcctgcg atctggtgtt aaagcagggg ctgaacttca
     3361 tttcctgaac cgcgaaatgt tacccggctg acgcggctct ctcctgccct tttccctgct
     3421 cctggacggt tttccccctg ttacacggga agcgaccgcg atcaaaaaag tctgtttata
     3481 aactaattcc taacaacatc gcattttttt gacactagaa ttgcagcaat atggcgacgc
     3541 cattacattg tgcttctcaa aaagcttaaa cgttggctaa acggcccggt ttagtgagga
     3601 tcgcccattg tcagaactac tctctttcgc cctttttctc gcctctgtgc tgatttacgc
     3661 atggaaagcg ggacgtaaca cctggtggtt tgcagccacg ttaacggtgc tggggctatt
     3721 tgtcgtttta aatatcaccc tgtttgccag cgactatttt actggcgatg gtattaacga
     3781 cgcggttctc tataccttaa ccaacagcct gaccggtgct ggcgtcagca aatacatcct
     3841 gccgggtatc ggcattgtgc tggggctgac ggcggtgttc ggtgcgctgg gctggatcct
     3901 gcgccgtcgt cgtcatcatc cgcaccattt tggttacagc ctgctggcgc tcttactggc
     3961 gctgggttcg gtggacgcca gcccggcatt tcgtcagata acggaactgg tgaaatccca
     4021 gtcacgcgat ggcgacccgg actttgcggc ttattataaa gagccgtcga aaactatccc
     4081 tgacccgaaa ctcaacctgg tttatatcta cggcgaaagt ctcgagcgga cctattttga
     4141 taacgaagct ttcccggatc tcacgcctga actgggcgcg ttgaaaaatg aaggcctgga
     4201 tttcagccac acgcagcagc tgccaggaac ggattacacg attgcgggca tggtggcttc
     4261 tcagtgcggc ataccgctgt tcgccccctt tgaaggcaac gcctccgcct ctgtctccag
     4321 cttcttcccg cagaacatct gtctgggcga tatcctgaaa aactcgggtt atcagaacta
     4381 tttcgtgcag ggcgcaaatc tgcgttttgc cggtaaagat gtgttcctga aatcgcacgg
     4441 cttcgaccac ttatacggct cagaagagct gaaaagcgtg gtagccgacc cgcactatcg
     4501 caacgactgg ggattctacg acgataccgt tctcgatgaa gcgtggaaaa agtttgaaga
     4561 gctttcccgc tcaggtcagc gattctcact gtttaccctg acagtcgata cccatcaccc
     4621 ggatggtttt atctcgcgca cctgtaaccg caaaaaatat gattttgacg gtaagccgaa
     4681 tcagtcattc agcgcggtaa gttgcagcca ggagaatatc gcgacgttta tcaacaaaat
     4741 caaagcgtca ccgtggttta aagatactgt catcgtcgtc tcttctgacc atttagcgat
     4801 gaacaacacg gcgtggaaat acctcaataa gcaggatcgc aataacctgt tttttgtcat
     4861 tcgtggcgac aagccgcagc aaaagacgct ggcagtgaag cgtaacacga tggataacgg
     4921 cgcgacggtg ctggacattc tcggtggcga taactatctc ggacttggtc gtagcagttt
     4981 atccgggcag tcgatgtcgg aaatcttcct caatatcaaa gagaaaacat tggcgtggaa
     5041 gccagatatc atccgcctgt ggaaattccc taaagagatg aaagagttca ccatcgacca
     5101 gcagaaaaac atgattgcct tctcgggtag ccatttccgt ttgccgctgc tgttgcgggt
     5161 ttcagacaaa cgcgtggaac cgctgccgga aagcgaatac tcagcaccgc tgcgtttcca
     5221 gctggccgat ttcgctccac gcgacaattt cgtctgggtt gacagttgct acaagatggc
     5281 acaactctgg gctccggaac tggcactttc caccgactgg tgtgtctcgc aggggcagct
     5341 tggcggtcag caaattgttc agcatgttga caaaacaaca tggaagagca agacggcatt
     5401 taaagatacg gtcatcgaca tggcgcgtta caaaggcaat gtcgatacgc tgaagattgt
     5461 tgataacgat attcgctaca aagccgacag tttcatcttt aacgtcgccg gtgcgccaga
     5521 agaggtgaaa cagtttagcg ggatttcccg tccggagtcg tggggccgct ggtccaacgc
     5581 gcagctgggc gatgaagtaa aaatcgagta caagcatccg ctgccgaaga aatttgacct
     5641 ggtgattacc gccaaagcat acggcaataa cgccagccgt cctattccgg tacgcgtagg
     5701 caatgaagaa caaactcttg tgctgggcaa tgaagtgacc accaccacac tgcatttcga
     5761 taacccaacc gatgccgaca cactggtaat tgtgccgccg gaacctgtct caaccaacga
     5821 agggaatatc ctcggacact cgccgcgtaa gctcgggatc ggcatggtgg aaattaaagt
     5881 ggtagaacgt gaagggtaat tcctgactga tgtgtaggtc gcatagggtg cgacctacac
     5941 gccttctccg gcttacaaca cattattaag aaacaaaaaa aacagcgccc aacacttccc
     6001 cggacgctgc atggcaactt taataatctc gccttatcag aaagtaatta cacccttaat
     6061 caactcacga ttgttaatca catcgcgctc gtaggtttct gccagcg
//
LOCUS       DAZXUK010000222         6093 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZXUK010000222 DAZXUK010000000
VERSION     DAZXUK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.222"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            12..479
                     /gene="ipgC"
                     /locus_tag="ACH3L8_003347"
     CDS             12..479
                     /gene="ipgC"
                     /locus_tag="ACH3L8_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFT4086326.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            485..2227
                     /gene="ipaB"
                     /locus_tag="ACH3L8_003348"
     CDS             485..2227
                     /gene="ipaB"
                     /locus_tag="ACH3L8_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFT4086327.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            2190..3338
                     /gene="ipaC"
                     /locus_tag="ACH3L8_003349"
     CDS             2190..3338
                     /gene="ipaC"
                     /locus_tag="ACH3L8_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFT4086328.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKPTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            3389..4387
                     /gene="ipaD"
                     /locus_tag="ACH3L8_003350"
     CDS             3389..4387
                     /gene="ipaD"
                     /locus_tag="ACH3L8_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFT4086329.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            4396..>6093
                     /gene="ipaA"
                     /locus_tag="ACH3L8_003351"
     CDS             4396..>6093
                     /gene="ipaA"
                     /locus_tag="ACH3L8_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFT4086330.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     "
BASE COUNT         2257 a         1238 c         1015 g         1583 t
ORIGIN      
        1 aaggagacct tatgtcttta aatatcaccg aaaatgaaag catctctact gcagtaattg
       61 atgcaattaa ctctggcgct acactgaaag atattaatgc aattcctgat gatatgatgg
      121 atgacattta ttcatatgct tatgactttt acaacaaagg aagaatagag gaagctgaag
      181 ttttcttcag gtttttatgt atatacgact tttacaatgt agactacatt atgggactcg
      241 cagctattta tcagataaaa gaacagttcc aacaagcagc agacctttat gctgtcgctt
      301 ttgcattagg aaaaaatgac tatacaccag tattccatac tggacaatgc cagcttcggt
      361 tgaaagcccc cttaaaagct aaagagtgct tcgaactcgt aattcaacac agcaatgatg
      421 aaaaattaaa aataaaagca caatcatact tggacgcaat tcaggatatc aaggagtaat
      481 tattatgcat aatgtaagca ccacaaccac tggttttcct cttgccaaaa tattggcttc
      541 cactgagctt ggagacaata ctatccaagc tgcaaatgat gcagctaaca aattattttc
      601 tcttacaatt gctgatctta ctgctaacca aaatattaat acaactaatg cacactcaac
      661 ttcaaatata ttaatccctg aacttaaagc accaaagtca ttaaatgcaa gttcccaact
      721 aacgctttta attggaaacc ttattcaaat actcggtgaa aaatctttaa ctgcattaac
      781 aaataaaatt actgcttgga agtcccagca acaggcaaga cagcaaaaaa acctagaatt
      841 ctccgataaa attaacactc ttctatctga aactgaagga ctaaccagag actatgaaaa
      901 acaaattaat aaactaaaaa acgcagattc taaaataaaa gacctagaaa ataaaattaa
      961 ccaaattcaa acaagattat ccgaactcga cccagagtca ccagaaaaga aaaaattaag
     1021 ccgggaagaa atacaactca ctatcaaaaa agacgcagca gttaaagaca ggacattgat
     1081 tgagcagaaa accctgtcaa ttcatagcaa acttacagat aaatcaatgc aactcgaaaa
     1141 agaaatagac tctttttctg cattttcaaa cacagcatct gctgaacagc tatcaaccca
     1201 gcagaaatca ttaaccggac ttgccagtgt tactcaattg atggcaacct ttattcaact
     1261 agttggaaaa aataatgaag aatctttaaa aaatgatctg gctctattcc agtctctcca
     1321 agaatcaaga aaaactgaaa tggagagaaa atctgatgag tatgctgctg aagtacgtaa
     1381 agcagaagaa ctcaacagag taatgggttg tgttgggaaa atacttgggg cacttttaac
     1441 tatcgttagt gttgttgcag cagctttttc tggaggagcc tctctagcac tggcagctgt
     1501 tggtttagct cttatggtta cggatgctat agtacaagca gcgaccggca attccttcat
     1561 ggaacaagcc ctgaatccga tcatgaaagc agtcattgaa cccttaatca aactcctttc
     1621 agatgcattt acaaaaatgc tcgaaggctt gggcgtcgac tcgaaaaaag ccaaaatgat
     1681 tggctctatt ctgggggcaa tcgcaggcgc tcttgtccta gttgcagcag tcgttctcgt
     1741 agccactgtt ggtaaacagg cagcagcaaa acttgcagaa aatattggca aaataatagg
     1801 taaaaccctc acagacctta taccaaagtt tctcaagaat ttttcttctc aactggacga
     1861 tttaatcact aatgctgttg ccagattaaa taaatttctt ggtgcagcgg gtgatgaagt
     1921 aatatccaaa caaattattt ccacccattt aaaccaagca gttttattag gagaaagtgt
     1981 taactctgcc acacaagcgg gaggaagtgt cgcttctgct gttttccaga acagcgcgtc
     2041 gacaaatcta gcagacctga cattatcgaa atatcaagtt gaacaactgt caaaatatat
     2101 cagtgaagca atagaaaaat tcggccaatt gcaggaagta attgcagatc tattagcctc
     2161 aatgtccaac tctcaggcta atagaactga tgttgcaaaa gcaattttgc aacaaactac
     2221 tgcttgatac aaataaggag aatgttatgg aaattcaaaa cacaaaacca acccagattt
     2281 tatatacaga tatatccaca aaacaaactc aaagttcttc cgaaacacaa aaatcacaaa
     2341 attatcagca gattgcagcg catattccac ttaatgtcgg taaaaatccc gtattaacaa
     2401 ccacattaaa tgatgatcaa cttttaaagt tatcagagca ggttcagcat gattcagaaa
     2461 tcattgctcg ccttactgac aaaaagatga aagatctttc agagatgagt cacaccctta
     2521 ctccagagaa cactctggat atttccagtc tttcttctaa tgctgtttct ttaattatta
     2581 gtgtagccgt tctactttct gctctccgca ctgcagaaac taaattgggc tctcaattgt
     2641 cattgattgc gttcgatgct acaaaatcag ctgcagagaa cattgttcgg caaggcctgg
     2701 cagccctatc atcaagcatt actggagcag tcacacaagt aggtataacg ggtatcggtg
     2761 ccaaaaaaac gcattcaggg attagcgacc aaaaaggagc cttaagaaag aaccttgcca
     2821 ctgctcaatc tcttgaaaaa gagcttgcag gttctaaatt agggttaaat aaacaaatag
     2881 atacaaatat cacctcacca caaactaact ctagcacaaa atttttaggt aaaaataaac
     2941 tggcgccaga taatatatcc ctgtcaactg aacataaaac ttctcttagt tctcccgata
     3001 tttctttgca ggataaaatt gacacccaga gaagaactta cgagctcaat accctttctg
     3061 cgcagcaaaa acaaaacatt ggccgtgcaa caatggaaac atcagccgtt gctggtaata
     3121 tatccacatc aggagggcgt tatgcatctg ctcttgaaga agaagaacaa ctaatcagtc
     3181 aggccagcag taaacaagca gaggaagcat cccaagtatc taaagaagca tcccaagcga
     3241 caaatcaatt aatacaaaaa ttattgaata taattgacag catcaaccaa tcaaagaatt
     3301 cgacagccag tcagattgct ggtaacattc gagcttaata tatccaagag ccataataat
     3361 atatggctct tcctgtaagg aaataaccat gaatataaca actctgacta atagtatttc
     3421 cacctcatca ttcagtccaa acaataccaa cggttcatca accgaaacag ttaattctga
     3481 tataaaaaca acgaccagtt ctcatcctgt aagttccctt actatgctca acgacaccct
     3541 tcataatatc agaacaacaa atcaggcatt aaagaaagag ctttcacaaa aaacgttgac
     3601 taaaacatcg ctagaagaaa tagcattaca ttcatctcag attagcatgg atgtaaataa
     3661 atccgctcaa ctattagata ttctttccag gcacgaatat ccaattaata aagacgcaag
     3721 agaattatta cattcagctc cgaaagaagc cgagcttgat ggagatcaaa tgatatctca
     3781 tagagaactg tgggctaaaa ttgcaaactc catcaatgat attaatgaac agtatctgaa
     3841 agtatatgaa catgccgtta gttcatatac tcaaatgtat caagatttta gcgctgttct
     3901 ttccagtctt gccggctgga tctctcccgg aggtaacgac ggaaactccg tgaaattaca
     3961 agtcaactcg cttaaaaagg cattggaaga actcaaggaa aaatataaag ataaaccgct
     4021 atatccagca aataatactg ttagtcagga acaagcaaat aaatggctta cagaattagg
     4081 tggaacaatc ggcaaggtat ctcaaaaaaa cgggggatat gttgtcagta taaacatgac
     4141 cccaatagac aatatgttaa aaagcttaga taatctaggt ggaaatggcg aggttgtgct
     4201 agataatgca aaatatcagg catggaatgc cggattctct gccgaagatg aaacaatgaa
     4261 aaataatctt caaactttag ttcaaaaata cagtaatgcc aatagtattt ttgataattt
     4321 agtaaaggtt ttgagtagta caataagctc atgtacagat acagataaac tttttctcca
     4381 tttctgaggt gcgccatgca taatgtaaat aatactcaag cgccaacatt cttatataag
     4441 gcaacttcac catcatcaac agaatacagc gagttaaaaa gcaaaatatc cgatatccat
     4501 agttcgcaaa cttctctaaa aacaccagca tcagtgtctg aaaaagaaaa ctttgcaacg
     4561 tcttttaatc agaaatgtct tgatttttta ttttcttcct cagggaaaga agatgtgtta
     4621 agaagcattt attccaactc aatgaatgcg tatgccaaaa gcgagattct cgaattttca
     4681 aatgttttgt actccttagt acatcaaaat ggtcttaatt ttgaaaacga aaagggactt
     4741 caaaaaattg tcgcacagta ttcggaacta attataaaag ataaattatc ccaagattct
     4801 gcctttggac catggtcggc aaagaataag aaactccatc aattacgaca aaacattgag
     4861 cacagacttg cactattagc acaacaacac acatctggtg aagctttatc attgggacaa
     4921 aaactcctca atactgaagt atcatcattt atcaagaata atattcttgc tgaattaaag
     4981 ttaagtaatg aaactgtttc atctctcaaa ctagatgatt tagttgacgc acaggcaaaa
     5041 cttgcctttg atagtttgcg caatcaacgt aaaaatacta ttgatagtaa aggatttggt
     5101 ataggtaaac tgtcaagaga cttaaataca gtagccgtgt ttcctgagct gttgagaaaa
     5161 gtccttaatg atattttaga agatataaaa gattcgcatc ctatccaaga tggcctccct
     5221 acacctcccg aagatatgcc agatggcgga ccaacccccg gagccaatga gaaaacatcc
     5281 caacctgtaa ttcactatca tataaataat gataatagaa cttacgataa tagagttttt
     5341 gacaacagag tatatgacaa tagctatcac gagaacccag aaaatgatgc acagtctcct
     5401 acttctcaga caaacgatct attatcccgt aacggaaact cattactaaa tccacaaaga
     5461 gcactagttc aaaaagtaac ttccgttcta ccacactcta tatcagatac tgtccagaca
     5521 tttgcaaata attcagcttt agaaaaggct ttcaaccata ctccagataa ttcggatgga
     5581 ataggttcag acctgttaac tacgagtagt caagaaagat ctgcaaataa ctctctttct
     5641 cggggacaca ggcctctgaa catacagaac tcttcaacca ccccccctct ccacccggaa
     5701 ggagtgacaa gcagtaatga taactcatca gatacaacta aaagtagcgc ttctctttct
     5761 catagagtag cttcgcaaat caataaattc aactcaaaca ctgattcaaa agtacttcag
     5821 actgattttt tatcaagaaa tggagacaca tatttaacac gggaaacgat atttgaagct
     5881 tcaaaaaaag taacaaactc cctaagtaat cttatatctc tcattggaac taaatcagga
     5941 acacaagaac gagagttaca ggaaaaatca aaggacatta caaaatccac aacagaacat
     6001 agaataaaca acaaattaaa agttacagat gcaaatataa gaaactacgt aacagaaacc
     6061 aacgcagata caattgataa aaatcatgcg atc
//
LOCUS       DAZXUK010000223         6088 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZXUK010000223 DAZXUK010000000
VERSION     DAZXUK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.223"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..84
                     /locus_tag="ACH3L8_003352"
     CDS             <1..84
                     /locus_tag="ACH3L8_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086331.1"
                     /translation="YESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="ACH3L8_003353"
                     /pseudo
     CDS             complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="ACH3L8_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(1611..2201)
                     /locus_tag="ACH3L8_003354"
     CDS             complement(1611..2201)
                     /locus_tag="ACH3L8_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFT4086332.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="ACH3L8_003355"
     CDS             complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="ACH3L8_003355"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFT4086333.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3179..3715
                     /locus_tag="ACH3L8_003356"
     CDS             3179..3715
                     /locus_tag="ACH3L8_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFT4086334.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3756..4616)
                     /locus_tag="ACH3L8_003357"
     CDS             complement(3756..4616)
                     /locus_tag="ACH3L8_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFT4086335.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="ACH3L8_003358"
     CDS             complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="ACH3L8_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFT4086336.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="ACH3L8_003359"
     CDS             complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="ACH3L8_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFT4086337.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1557 a         1543 c         1509 g         1479 t
ORIGIN      
        1 tatgagtcag tatgcatcgc cgcgatggaa catttaaaaa gtaaagcaac ggcccctata
       61 caaaacggac cgttgccagc ataagaatta acggtgtgcc agttcggcgt cgtcttcgct
      121 atccagaatg gctttatcgg tttgcttcag ccactggctg gtcagcgtgc cagctgtcat
      181 cgagccacta acgtttagcg ccgtacggcc catgtcgata agcggttcaa cggagattaa
      241 cagcgccacc agggttactg gcaggcccat cgcaggcagc acaatcagtg cggcgaaagt
      301 tgcaccacca ccgacaccgg caacgcctgc ggaactaacg gtaacaatac cgaccagcgt
      361 cgcaatccac atcgggtcca gcgggttaat gccaaccgta ggcgcaacca tcaccgcagc
      421 attgccggat acaaaccggc gcagccgttc tgaccaatgg ttgcaccgaa agaggcggcg
      481 aaactggcga tggattcagg aacgcccaga cgacgcgttt gtgcttccac attcagtggg
      541 atagacgcag cactggaacg gctggtaaag gcaaacgtca gcacaggcca taccttacgg
      601 aagtacttca gcggactcac gccattaatg cccagcagaa tgccatgcac agcaaacata
      661 atcagcagac cgaggtagga cgcgacaacg aaacttccca gtttgatgat gtcttgcaag
      721 ttagaacctg caaccacttt ggtcattaga gccagaacgc cgtaaggggt caactgcatg
      781 acaaggcgaa ccagtttcat cacccagctt tgtagggtat cgatagcggt taagacgcgt
      841 tcccctttcg gcgcatcatc cttcagcagt ttcaatgcag ctacgccgag gaatgcggca
      901 aaaattacca cgctgataat tgacgtcgga ttggctccgg taagatcggc aaacgggttt
      961 ttcgggataa aggacaagac cagctgcgga acgctctgat cagagacttt accaacatag
     1021 ttactttcaa tggcgttcag acgtgcagtt tctgcaccac cctgaaccag accttcagcc
     1081 gtcaaaccaa acaggttggt gaccagcaca ccgaccagcg ccgcaatcag cgtggtaaac
     1141 aaaagcgtac cgatggtcag aaaactgatt ttgcctaact gagatgcgtt atgcagacgg
     1201 gcaaccgcgc tcagaataga ggcgaagact aacggcataa cgatcatttg cagcagttga
     1261 acatagccgt taccaacgat gttaaaccac tgtacagaat ctttaagtac ctggctgtca
     1321 gaaccataaa tggtatgcag ggcaaggcca aaaaccacac ccatcaccag acccaccagc
     1381 acttttttcg ccagactcca ctgtttatgg cgggtctgag ccagcgcaaa cagcagtaca
     1441 acgaacacca cgatgttcgc aattaatgga aagttcatcc ccgttctcct gatcttatta
     1501 tttctatcac tgcgtagcag tcatagtgtt ggcgcaagat taacagaact gtgagctggc
     1561 tcttatattc aaatggaatt gcttattcca gaaaggtgat ttttgccttt ttagtattca
     1621 acctgatgct taataagccg atgaaactgg atctgcaagg tattgatcat ttgcgatgac
     1681 caacgaacag cgctgttctc gggtaatgaa tggggcatcc ataccgacca gacaaataat
     1741 gccatgcaga taaaacgttc cagttggttg cccttttggg gaaccaggat aggcaagcgg
     1801 aaacgccagc ggcatggcca gagcagggga acaccggcgg gtgtcagcat atcggcaagt
     1861 atgtggctca aataacccag caccattcct tgtagcgcat cagccggaat gaaccagcct
     1921 tccggaacct taaggtaaaa ggttgccagc agcgcaaata ccgccagcag actgtgggta
     1981 aaaccacgat gcccaaaagc gcgggcgatc ggttttgata tccattttaa tcgctgccca
     2041 agaaacgact ttgggtgatc gatgtccggt aacaaacacg tcaggattgc ggaagggaca
     2101 atatgccacc agtcaccctg tgccagcacg ggcgtcagct cggcattttt ggcaaatacc
     2161 gcacaagcaa tagaaaagag aaggtgacct tccgccgtca tgataaaact cactaaactg
     2221 tcgatacgta cagtatagga tttttataca gtgcacggaa gtggtgacgg tgtaactctt
     2281 tctcttaaat tgacgattaa tccgtcagtt gaatgcaaat tcagataaag aaccaggggc
     2341 agcgtgatca ctgcccctgg tcattaaccg agaaggtctt ttgcggtgag ttctgtcagc
     2401 gatgagagtt tgacgtttgc taatacaaaa cgtggatcat tttgcgcttc tggcgcagga
     2461 acgacgatgg aacgcatgcg ggctgcttta gaagcgatca tgccatttac cgagtcttcc
     2521 agcgccacgc aggtcagtgg gtcaacgccc agttttgctg cgcagtcgag atatacttgc
     2581 ggatgcggct tgctgtaagg cagtttttca gccgaggcga gggcatcgaa actgtcgcgt
     2641 aagtcaaaca tggtcaacac tttttccagc atatgtagtg gtgacgcgga ggccagtccc
     2701 accaataaac cttgttcttt gcataacgca acagcttcgc gcacgcctgg taataatgga
     2761 cgtgtctctt caaccagtga aatggcacgg gcaataaccc gttctactac ttcctgacgg
     2821 cttggcccat tccacggttg ccgggcgtac caaagatcga ccaccatatc gatgcgtaaa
     2881 cctaaggtgt ccggcagctc gttacgacgg gagatatcta cccccaggct tgccatcaca
     2941 tccagttcgg ctcgatccca taaaggttct gagtcgataa gtaatccatc catatcaaaa
     3001 attgcagcaa gaatctgacg cggggttgac attacaacct ctcctttaga gcattatgtg
     3061 taaggtatta agccgtgctt atgattaagc gcctatcata tcgcctttaa agaatgggcg
     3121 aaaacagata tctacaggta aactaagtga aacgacgcgt cttaatcaag gagaactcat
     3181 gacgtatcaa caagctggac gcattgctgt tttgaaaagg attttgggtt gggtgatttt
     3241 tatacctgca ttgatctcta cattgatttc tttgctgaaa tttatgaata cccggcagga
     3301 aaaccaggaa ggcattaatg cggtcatgct cgactttgct catgtcatga tcgatatgat
     3361 gcaggcgaat acgccttttt tgaatctgtt ctggtataac tccccgaccc ctaattttaa
     3421 tggtggtgtg aacgttatgt tctgggtgat tttcatcctg atttttgtcg gactggcgtt
     3481 gcaggattcc ggtgcccgga tgagtcgcca ggcgcgcttc ttgcgggaag gtgttgaaga
     3541 tcaactcatt ctggaaaaag ccaaagggga agaggggcta acgcgtgaac aaattgagtc
     3601 ccgtattgtt gttccgcacc atactatttt cctgcagttt ttctcactat atatcctgcc
     3661 ggttatttgt attgctgcag gttatgtgtt cttttctctg cttgggttta tttaatcaaa
     3721 aggccgcacg gagcggccat tatcctcaac ccatatcatg ctgctaataa tctatccaat
     3781 gactgctgag caataaccaa atgctgaccg ccaaataatc ttgcacgatt taacagcgtg
     3841 tagagttggt aaaccggttg acgttcaagg aaatcggcag gtagcgggga tactgactga
     3901 tagccgtcat agatttgtgg cggctgttca gtatgcagcg gtaacatcgc taggtcgcac
     3961 tctcggtcac cccagtcgca ggcagggtcg aaaatgtacg ggccatccgg acccagcgca
     4021 cagttgccgg accataaatc gccgtgcaac agagaaggct gcggttgatg cgaggccaga
     4081 cgctgctgaa tatgctcgac gatggcgtcg atattgccga aagcgatccc tttctctgct
     4141 gccagttcca actgccagcc aatccgttgt tcagcaaaaa acgttgacca gcgacgttgc
     4201 caggtgttgg gctgtggagt tgtggagagc gagttatcaa aatcgaggcc aaattgtggt
     4261 tggtcactcc attgatgtaa acgcgcaatt tgctgaccaa gaataaatgc gctatgcgca
     4321 tccagcggac gaggtgggag ataatccatc accagaaaac tgtagtcacg gtcagcgcct
     4381 actgcccaaa ccttaggcac ggtgacggtt ttactacgcg acagtaactc cagttggtcg
     4441 gcttcggcgg taaaaccggg aagcagttcc ctttcatcac atttgacgaa aaagtcatgt
     4501 cctgcatagc gcaaatgcca tgcggcatgg acttctccgc caggcagttc attacgcagt
     4561 tcgatttcgc cttcacctaa ctgctcgctc aaaagacgac tgattgcctg ccacatgatg
     4621 tctctcccat gttgtctgcc agatcataag gttagcgcaa gaatgcggtg taaaaacacg
     4681 agctaacgca caccacagac tgattattgc ccaaaaacca tttattttct gttgctattc
     4741 cattcctccc aggtcgtgat ggtgatttca ggatctttgc cggttgcact ttgggtaagc
     4801 cccgaaacca gttctctaat ttcttcttca ctttgggtac tgataagtcc aaaagtgtta
     4861 gtccccagct catggatatt tccctcatca tccgtcatgg tgagcaaaaa accgtcgcga
     4921 gtcaggtaat tattcaactc gttaatttca gtcaatgtat cctcatgcaa catgacggtt
     4981 atgacataat ggacaagatc gccactggcc atagttcacc cctttgttat cacatgactt
     5041 ttgagcatag tcggagaaac gcgttgccga caggttggtg gtgattcccc caatgctggg
     5101 ggaatgtttt tgttagcggg aaaggtaagc gtaaattttt tgcgtatcgt catgggagca
     5161 cagacgtgtt ccctgattga gtgtggctgc actccccgca gctacgccaa aacggaccat
     5221 ctcttcaaga gaggcatttt ctgccagttt cagtgtcatc gcgccgacca tgctgtcacc
     5281 agcgccaacg gtactctggc ttttcaccgg tggtggcacc acctgaatgc agttttcact
     5341 atcgacaccc agcgctcctt gtggaccaag ggaaacgaca acccgtttgg ccttgccgct
     5401 attaacgatt tcctgcgcgg ctttgcggac atcgtctggc tgggtgagtt cgcgattcac
     5461 cagcgcactg agttcttttt ggttaggctt aaccaactcg atgttaccaa tcgccagtgc
     5521 tgcacttaac gcttcgccag aactgtcgat gatgcagcgg atcccttggt tttgcgcagc
     5581 ggaaatcagt tgggttaatt tttccagctt cacacctggc ggcaggcttc cgcttatgac
     5641 caggatggcc ccggattcaa tttccagaac ttgctcttca agctggcgaa actcatcttc
     5701 atttaatgcc gcgcctggca taacaaaacg atactgttca ccgcttgctt ccacatgtac
     5761 gtgtaaattc tgccgggtcc agtctttggc ttctacagta gcgacgggga cattttcatc
     5821 cgccaacagt gaaaccaggt gttcgccggt cgcgccaccc gccgggaaga tcgctgtggc
     5881 actgcctcca agatgggcaa tggcgcgggc gacgttgatg ccgccgcccc cgggttcgaa
     5941 caccggtgcg gtacagcgca gttttccttc gggataaatt tgcggggtaa ttgttgcgct
     6001 atcgagagag ggcgcaagtg tcaacgtata gatacgtacc atcatttcct cctataggct
     6061 gatttcagtc tggcaccgaa tcagcgga
//
LOCUS       DAZXUK010000224         6058 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZXUK010000224 DAZXUK010000000
VERSION     DAZXUK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.224"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..143)
                     /locus_tag="ACH3L8_003360"
     CDS             complement(<1..143)
                     /locus_tag="ACH3L8_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HFT4086338.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="ACH3L8_003361"
     CDS             379..651
                     /locus_tag="ACH3L8_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFT4086339.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACH3L8_003362"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACH3L8_003362"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFT4086340.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1731
                     /locus_tag="ACH3L8_003363"
     CDS             1111..1731
                     /locus_tag="ACH3L8_003363"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFT4086341.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="ACH3L8_003364"
                     /pseudo
     CDS             complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="ACH3L8_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3114..3767)
                     /locus_tag="ACH3L8_003365"
     CDS             complement(3114..3767)
                     /locus_tag="ACH3L8_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFT4086342.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3767..5238)
                     /locus_tag="ACH3L8_003366"
                     /pseudo
     CDS             complement(3767..5238)
                     /locus_tag="ACH3L8_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5211..6058)
                     /locus_tag="ACH3L8_003367"
                     /pseudo
     CDS             complement(5211..6058)
                     /locus_tag="ACH3L8_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1621 c         1599 g         1411 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt
     1681 acgcgggtgt ggagtgggta tctgacgctg aagtcgctcc agcgtcagta attgcaatgt
     1741 tatttgctac tgcccgggcc gcgttcaccg gttgcaaccg gattttttgg atcgctgctc
     1801 cattcgtacc agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac
     1861 ataaaggttt cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgatctggt
     1921 ttgatattcc atgctttcca catagcggta atatcatcgg cgctacgcat ggtgccatcc
     1981 gggttataga aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg
     2041 gctatttcac cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc
     2101 cacgaacgaa tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc
     2161 atatcgagca tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc
     2221 actttcggtg gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg
     2281 ccatccagca gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct
     2341 ccagcgtata cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt
     2401 gccagcatcg cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact
     2461 tcgttggtat cgatgtagtc agcgccagga atatggctga taaggtaaag cttaggagcg
     2521 ccctaggccg cttcaatgac tttccagtca ccgacaggtt tcgccgtaac ctctttacct
     2581 tgttgcaggt catgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt
     2641 agacgggaag gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt
     2701 tgcagtcgcg ttttgacggc gtcaacatct ttgccattac cgtacagcgc caccggggca
     2761 tcggctttca ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcc
     2821 agccagctag cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt
     2881 tgtggccagc cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgc
     2941 tgaagttggt caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc
     3001 agccctaaag ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac
     3061 attgtagttt tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc
     3121 agtttgttgt aattttctga ccactgcgcc atatccagaa cagaactatc agcaggaaca
     3181 tcctggagat cgtgcgttac ctgaacgacg gggatcgcca gggcgcgaac ttcgctgaac
     3241 acccactggc gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg
     3301 agtaacgctt ttggctgggc gagaagggcg cgtagtagag caacgcgcgc tcgctgaccg
     3361 ccagacaaag tggcaggatc ctgatggaaa gctccgtcaa ggcctgaacg ctcaagtgca
     3421 tcattcacgg cattacgtcg ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa
     3481 ttttgcccga cgctgaactg gtcaaataac agtgcatcct gaaaaagaat gccaatctga
     3541 cgctgtgcgg tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa
     3601 gaaaactgtc cggccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca
     3661 gacggcccca ttaacgtgac gatgtcacct ttatcaaccg taaagttaac gtttgtcagc
     3721 aagcggcttt ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggattcctt
     3781 gtctgacata acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta
     3841 acaacagttg ccataacgcc tgggcggcga gaataccgtt gctgccgccg ctgcttaatg
     3901 ccaccgcctc agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat
     3961 actgcgcaat actgactgaa aatcccaccg cgaaggcaat cagcacaggg cgcaacatga
     4021 gtgggcattt cacgtaaaag aagattttgg cccgcgacca gcccagtgtt tgcgcaatca
     4081 aaattaaccg tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca
     4141 ttacccacag cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata
     4201 gcgccagcgt gtactggccc gccaccagtg gcagagcggg caatagaatg ggcaaccata
     4261 gccacaactg gcgacgctgt gccccccatt ccagccacag cagtaacagg agcaaagcga
     4321 tgaatgttgc taccagcccc attgtcagac tgttgatgag tgcttcacta ttgatcgtcg
     4381 actgatccgc gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac
     4441 tcgccagcgt agtgcctggt aataaaggtg tggcaggctt gcgaatgcca tctacgcggg
     4501 gaatagtgcg ccgccagctg cgccaaaacg taggcggcga gtaacagcat cagcagcaga
     4561 ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct gagttaacca ctgccagcta
     4621 attactgcca gcgtcggtgg attacctggc ccaagaataa tcgccacatc cacgaccgaa
     4681 agcgaccagg caacaatcgc cagcatcgcc attgccagcg gctgtagccc agcgaatcca
     4741 gcacaatgac ctgctgcaac agccgttttt cgctcaatac tgccactaag atccacagca
     4801 gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatccaaatc ggtccattgg
     4861 cggagtgaaa tacgggaaat agtcatatag cagccctccg tcagcaaaga gcagcagggc
     4921 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg cgcacatacg
     4981 ctgccatttc ggcccaggcc atagcgccac aatcaccagc agggcaatca acaatgcccc
     5041 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     5101 ggcaaataac gcctgccagc gtgtcaacga caacgcaggc gatatcagcg tgagagctgc
     5161 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5221 ccgtaacggc gtaaccattc ttgttccagt gcattcaccc atcctgcgtg cggttcagcc
     5281 agtaccggcg gcagatcctg cggcattctt gattgcaata tttcgcgctg cccgtcaggc
     5341 agtttttgcg gatcgagaac agaaggatcg ccccagacaa cgggatctgc tttacgcagt
     5401 tgcgcatcgg gtgagagcag gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5461 ttggctggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5521 taactgcttg ccggcaaatc gccgctggcg attttttgct gcgcatgtgc ggggttaaag
     5581 gtcagcgaca ggcgcaatgt gccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5641 gggaaatcct ttccttcgcg ccacaaatac ggatgcagtg catcaagata ttgctacaag
     5701 ggcgcagtga cacgggcgaa agtcgcatcg tccggcgctt cttttaatgc ggcgggatct
     5761 ggcgtcagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5821 ggataagtaa ccgtgccggg attggcttta gcaaactcca gtaaggcttg cggcgtttgt
     5881 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc
     5941 gattccgcac cttgtgtcgg cactgaaaaa tctccccgca ccggcagctg tgtgtcgaca
     6001 tagcgccagt tgggcagagt ctccgcccag cccgtttgta ataaattggc ctctttta
//
LOCUS       DAZXUK010000225         6035 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZXUK010000225 DAZXUK010000000
VERSION     DAZXUK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.225"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            413..1321
                     /locus_tag="ACH3L8_003368"
     CDS             413..1321
                     /locus_tag="ACH3L8_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFT4086343.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMLPGSTVPFISTK"
     gene            complement(1367..3358)
                     /gene="tkt"
                     /locus_tag="ACH3L8_003369"
     CDS             complement(1367..3358)
                     /gene="tkt"
                     /locus_tag="ACH3L8_003369"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFT4086344.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3636..4394
                     /gene="loiP"
                     /locus_tag="ACH3L8_003370"
                     /gene_synonym="yggG"
     CDS             3636..4394
                     /gene="loiP"
                     /locus_tag="ACH3L8_003370"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFT4086345.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4600..5520)
                     /gene="speB"
                     /locus_tag="ACH3L8_003371"
     CDS             complement(4600..5520)
                     /gene="speB"
                     /locus_tag="ACH3L8_003371"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFT4086346.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5656..>6035)
                     /locus_tag="ACH3L8_003372"
     CDS             complement(5656..>6035)
                     /locus_tag="ACH3L8_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086347.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1484 a         1575 c         1557 g         1419 t
ORIGIN      
        1 attgatgatt ctggaaaata atcactaagc cgcataaatt atccttattt tatttttagg
       61 gtaccgtccg gcggtaaaaa taaaatactc gtgcggttta atgaaagcaa ttgggtacgc
      121 gagaagaaaa accccacaaa ccaaaataat aaaaccttaa ataacaacaa tatgaaattg
      181 atatttttaa agtcaacttt gtatttgtta tgttcatcac aaaatcaaca ttttgtttgt
      241 tattgtattt gtgatgcata tctctattta tttattgaat gttcgaaaac atacttttct
      301 aatgcaatgt tttttcgcta taccccttta tagattctga attctcacct gtcggctgtt
      361 ttctcctcat ctcatgtgac atattcccat actttactca aggaagattg tcatgaaagc
      421 acctgtttca ttaacctcat ttcgcccaca aaaatctctt gctatcgcca ttggcgtgct
      481 tgccgtggtt gtgctgccat ttctctcgta ttacaccgtt aatgaaggtg aacgcggcat
      541 tttgctgcgc tacggaaaaa tcgtaaaagt tgccgaaccg gggctgggtt ttaagatccc
      601 ttttatggaa agcgtggaga aaatttccac gcgtaatcag gctgtggtct atcagggatt
      661 acaggcctac agccgcgacc agcaaccggc acaaatgaca gtatcagtca gcttccacat
      721 taaaccttca gaagcaggcg cggtttatac cacctacaac actattgaag cattaaaaga
      781 tcgtctaatt gtccgtcagt tgccaactca gctggagaac attttcggcc aatataccgc
      841 catcagcgcc gtacaggatc gtacaaaatt ggttcaggat ctgcaaaatg ccatgcgcaa
      901 agccgttgtt gggccagtgg taattgacgg ggtccagatt gaaaatattg atttttctga
      961 cgcttatgaa aagtcgattg aaaaccgaat gaaagcagaa gtcgccattg ccacgcgtaa
     1021 gcaaaatctg gaaacagaga aaatccaggc gcaaatagcg gtaactcagg cccaggcaga
     1081 agcagacagt aaactggcgg cagccaaagc agaagcggaa actattcgcg taagaggcgc
     1141 ggcagaggct gaaactattc gtttaaaaag tgcggctgaa gcagaagcaa tccgcctgcg
     1201 cggtgaagct ctgcgggata atcccggact tgtagccctc accactgccg aacgttggga
     1261 cggtaaactg cccgatacca tgctccccgg cagcacagtt ccgtttatct ctactaagta
     1321 aaaaaagggc cgctttcgcg gtccttctca caaaacgtct tactgattac agcagcgctt
     1381 ttgctttcgc aacaacgtta tcaacagtga agccgaactc ttcaaacagc agctctgccg
     1441 gagcagattc accgaaggtg gtcatgccga cgatagcgcc gttcaggcca acatacttgt
     1501 accagtagtc agcaataccc gcttctaccg caacgcgtgc agtaaccgct ttcggcagta
     1561 cggattcacg gtaagccgca tcctgcttgt caaatgcgtc ggtagacggc atggacacca
     1621 cgcgcgcttt cacgccttcg gcagtcagtt tttcgtaggc agcaaccgcc agttcaactt
     1681 cagaaccggt ggcgatgaag atcagttccg gctgaccagc acagtctttc agcacataac
     1741 caccgcgcgc gatgtttgcc agctgctctt cagtacgttc ctgctgcgcc aggttctgac
     1801 gggagaggat aagcgcagtc gggccgtcct gacgctcaac gccgtatttc cacgcgaccg
     1861 cggattcaac ctggtcacac ggacgccatg tagacatgtt cggggtcacg cgcagagaag
     1921 cgacctgctc taccggctgg tgagtcgggc catcttcgcc cagaccgatg gagtcgtggg
     1981 tgtaaaccat cacctggcgc tgtttcatca gcgcagccat acgtacggcg ttacgtgcgt
     2041 attccacgaa catcaggaag gtggaggtgt acggcaggaa gccaccgtgc agggagatac
     2101 cgttagcaat cgcggtcata ccgaactcgc gaacaccgta gtggatgtag ttacctgcag
     2161 catcttcgtt gattgcttta gaaccagacc acagggtcag gttagacggt gccaggtcag
     2221 cagagccgcc gaggaattca ggcaacagcg ggccgaacgc ttcgatagca ttctgcgacg
     2281 ctttacggct ggcgattttc gccggattag cctgcagttt agcgataaac tctttcgctt
     2341 tggcgtcgaa gtcagacggc atttcgcctt tcatacggcg ggtaaattca gccgcttcct
     2401 gcggataagc tttcgcgtaa gccgcaaact tctcattcca tgcagattct ttcgcctggc
     2461 ctgcttcttt cgcatcccac tgagcataga tttcagacgg gatttcgaac ggtgcatatt
     2521 tccagcccag ctgttcgcgg gtcagggcaa tttcagcgtc gcccagcggc gcaccgtggg
     2581 agtcgtgagt accggctttg ttcggggaac cgaaaccgat gatggttttg cacatcagca
     2641 gggacggttt gtcagtcact gcgcgcgctt cttcaactgc gcgtttgatg gatgccgcgt
     2701 catgaccgtc gatgtcgcga ataacgtgcc agccgtaagc ttcgaaacgc attgctgtgt
     2761 cgtcggtgaa ccagccttca acgtgaccat cgatagaaat accgttgtca tcgtagaacg
     2821 caatcagttt acccagcttc agcgtacccg ccagagagca aacttcgtgg gagatgcctt
     2881 ccatcatgca gccgtcgccc atgaaggcgt aggtgtagtg gtcgacaatg tcgtggcccg
     2941 gacggttaaa ctgcgccgcc agcgtttttt ctgcaatcgc cataccgact gcgttggcaa
     3001 taccctgacc cagcggaccg gtggtggttt ccacaccagc ggtataacct acttccggat
     3061 ggcccggagt tttagagtgc agctgacgga agtttttcag ttcttccatc ggcagatcgt
     3121 aaccggtgag gtgcagcagg ctgtagatca gcatggagcc gtggccgttg gacagcacga
     3181 agcggtcacg gtcagcccag gacggattct gcgggttgtg tttcaggaaa tcacgccaca
     3241 ggacttcggc aatgtcagcc atacccatag gggcacccgg gtgaccggat ttggctttct
     3301 gtactgcgtc catgctcagc gcacgaatag cattggcaag ctctttacgt gaggacattt
     3361 tgactccaga tcggatgatg aagggcacgc ccttaacgac ttgacgacag cgcgttttgg
     3421 gctacgccgg aaaatttgcc aacaatttac cgcaagccgc gcgtcatgta catggaacat
     3481 ccttttgccg cttcagaaat ctctggatca tgctcgcatg ttgcgcaatc tgctcgcccg
     3541 tccgctgcgc ttttccttat actgagactg agcgtcgatt cacctgcaaa cggcgcattt
     3601 ttagaataat cctgaccttg tgcggaagag aaaacatgaa aattcgcgcc ttattggtag
     3661 caatgagcgt ggcaacggta ctgaccggtt gccagaatat ggactccaac ggactgctct
     3721 catcaggagc ggaagctttt caggcttaca gtttgagtga tgcgcaggtg aaaaccctga
     3781 gcgatcaggc atgtcaggag atggacagca aggcgacgat tgcgccagcc aatagcgaat
     3841 acgctaaacg tctgacaact attgccaatg cgctaggcaa caatatcaac ggtcagccgg
     3901 tgaattacaa agtgtatatg gcgaaggatg tgaacgcctt tgcaatggct aacggctgta
     3961 tccgcgtcta tagcgggctg atggatatga tgacagataa cgaagtcgaa gcggtgatcg
     4021 gtcacgagat ggggcacgtg gcgttaggcc atgtgaagaa agggatgcag gtggcgcttg
     4081 gtacaaatgc cgtgcgagta gctgcggcct ctgcgggcgg gattgtcggc agtttatctc
     4141 aatcacaact tggtgatctg ggcgagaaat tagtcaattc gcaattctcc cagcgccagg
     4201 aagcagaagc cgatgattat tcttacgatc ttctgcacca acgcggcatc agcccggcag
     4261 gtcttgccac cagctttgaa aaactggcga agctggaaga aggtcgtcaa agctcaatgt
     4321 ttgacgacca tcctgcatcc gccgaacgtg cccagcatat tcgcgatcgc atcagcgcgg
     4381 atgggattaa gtaaagccta gtggtgtcga acgtaggtca gataaggcgt tcacgccgca
     4441 tctgacatta atggcacatt tgttaccttg tgcgcattgc cggatgcgat gctggcgcat
     4501 cttatccggc ctacgggtgc cgaacgtagg tcggataagg cgttcacgcc gcatccgaca
     4561 ttaatggcac gttttacccg tgcgcatcgc atctggtgct tactcgccct ttttcgccgc
     4621 ctgaatatac agcatctcca gcgccagcgt cgccgcagcc agagcggtga tttccgactg
     4681 atcgtatgcc ggagccactt ccactacgtc catcccaacg atgttgagat ctttcaggcc
     4741 gcgtaccagt ttaatggcgc gatcggaggt caggccgcca atcactggcg taccggttcc
     4801 cggcgcgaaa gcaggatcca ggcagtcgat atcaaaagtc aggtaaaccg gcatatcacc
     4861 cacaatctgt ttcacctggg cgataacgtc atccacgctg cgatcgttca cctggcaggc
     4921 gtccagcacg gtaaagccgt tgtctttatc aaactcggta cgaataccaa tctgcacgga
     4981 atgattcggg tcgatcagac cttctttcgg cgcggtatag aacatagtgc cgtggtcaaa
     5041 ttcacaaccg ttcgcatagg tatcggtgtg ggcgtcaaag tgtaccagcg ccattttgcc
     5101 gaaatgcttc gcatgagcac gcagcagcgg cagcgtaaca aagtggtcac caccgaagga
     5161 gagcatacgc ttaccggcag ccagcagctt ctcggcgtgc gcctgcagct tttcgctcat
     5221 ctcacgggca tcgccaaagg catataccag atcgccgcag tccacgacgt tcagacgctc
     5281 acgcatgtcg aaattccacg ggaagcggtt gtgttcccag gccagattcg tcgaaacctg
     5341 acggatcgct gccggaccgt ggcgaccacc cgcacgacca gaagtggcca tatcgaacgg
     5401 cacgccagta atcacccagt ctgcatcgct gtcatacggc tggaagttca tcggcaggcg
     5461 taaaaaacca aaggcattgg aaaccagtga gttatcgtat tgatgaccta aggtgctcat
     5521 ggatggactc ctattacaaa gtcgatataa aaaaaccctt ccgcgtcgtt aggcccgacg
     5581 aggaagggtt ggatttgcga aacacaagtt tgtgctaatt atcgccttaa atgggcgagg
     5641 catcaagcga ggttgctatt tccgacacgg tggcggcgca tagccattgt tatctgccgg
     5701 aaaggcttgt ccagagcacg gcggcagcgc gttttgcgcc gggccttgta attcaaaggc
     5761 ggcaaagccg attaccccgg tattacgcac atcgccttgc gccgaattgg ctgcgtagga
     5821 atcagcaacg ttggcgaact gaaaagccgc ttcagtatgt ttatctttgc ggaatccttt
     5881 aatcactaac gaatctccgg cattcacaat atagccgttg ttattcagcg aaccttgctt
     5941 gccgttcagc acatccaggc catcagccgt agcaacgatc tcgtagttgg tgttacgact
     6001 gtaattacgg acatataatt gataattcat tccga
//
LOCUS       DAZXUK010000226         6019 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZXUK010000226 DAZXUK010000000
VERSION     DAZXUK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.226"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1094
                     /locus_tag="ACH3L8_003373"
     CDS             <1..1094
                     /locus_tag="ACH3L8_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFT4086348.1"
                     /translation="KIAPDSWVIDVDQILENGKRLIETARLYGIELYLMTKQFGRNPW
                     LAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQGHLVQIPCHQVADAVEQGTDIITV
                     FTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLYPGQESGFVQHSLHEVIAEIKKLPG
                     LHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQARDQLAKSGIAIEQLNVPSATSCTSL
                     PLLAEYGATHAEPGHALTGTIPANQQGDQPERIAMLWLSEISHHFRGDSYCYGGGYYR
                     RGHAQHALVFTPENQKITETNLKTVDDSSIDYTLPLAGEYPVSSAVVLCFRTQIFVTR
                     SDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            1095..2321
                     /locus_tag="ACH3L8_003374"
     CDS             1095..2321
                     /locus_tag="ACH3L8_003374"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFT4086349.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            2318..3196
                     /locus_tag="ACH3L8_003375"
     CDS             2318..3196
                     /locus_tag="ACH3L8_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFT4086350.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            3207..3560
                     /locus_tag="ACH3L8_003376"
     CDS             3207..3560
                     /locus_tag="ACH3L8_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFT4086351.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            3572..4876
                     /locus_tag="ACH3L8_003377"
     CDS             3572..4876
                     /locus_tag="ACH3L8_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFT4086352.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            4888..5973
                     /locus_tag="ACH3L8_003378"
     CDS             4888..5973
                     /locus_tag="ACH3L8_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFT4086353.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
BASE COUNT         1296 a         1475 c         1763 g         1485 t
ORIGIN      
        1 gcaaaatcgc cccggacagc tgggtgatcg acgtggatca gatactggaa aacggtaagc
       61 ggctcattga gacggcgcgg ctttacggca ttgaactgta tctgatgacc aagcaatttg
      121 gtcgtaatcc gtggctggcg gaaaaattgc tggcattagg ctacagcggc attgtggcgg
      181 tggattacaa agaggcgcga gtcatgcgcc gcgctggttt gccagtggcg catcaggggc
      241 atctggtaca aatcccttgt catcaggttg ctgacgccgt tgaacagggc accgacatca
      301 tcaccgtgtt tactctcgac aaagcgcggg aagtttctgc ggcagcggtt aaggccggac
      361 gggtccagcc tgtgctgctc aaagtttata gcgacgatga ttttctttat ccggggcagg
      421 agagcggttt tgtacagcat tcgctgcacg aggtgattgc tgaaattaag aaactgccgg
      481 ggctgcattt agccggacta acccatttcc cttgcctgct ttgggatgag gctgctggga
      541 aagttttgcc aacaccgaat cttcacacgc tggtacaagc acgggatcaa ctggcgaaat
      601 ctggtattgc gattgagcaa ctgaacgtac cttcagcgac cagctgcact tcgctgccat
      661 tgctggcgga atacggtgcg actcatgccg aacccggtca tgcgctgacc ggtactattc
      721 cggcaaacca gcagggcgat cagcctgaac gtatcgcgat gctctggtta agtgaaatct
      781 cccatcattt ccgtggcgac agctactgct acggcggcgg ttactaccgt cgtggtcatg
      841 cgcaacatgc gctggtgttt acgccagaaa atcaaaagat tactgaaacc aatctcaaaa
      901 ctgtggatga cagcagtatc gactacaccc tgccgctggc aggcgagtat ccggtgagca
      961 gtgcagtggt gctctgtttt cgcacgcaga tttttgtcac ccgtagcgat gtggtgctgg
     1021 tgtccggtat tcagcgcggc gaaccagaaa tagttggtcg ttatgacagc ctcggcaacc
     1081 ctctggaggc ataaatggcg cgatttgtgg tgttagtgat tgatagcttt ggcgtagggg
     1141 caatgaaaga tgtcacgctg gtgcgcccgc aagatgccgg ggcgaatacc tgtggtcaca
     1201 tcctgagcca gttgccgcat ttgcagctac caacgctgga gaagctgggg ctaatcaaca
     1261 cattgggtta tgcgccaggc gatatgcagc cgtcagattc cgcaacctgg ggcgtggcag
     1321 agctgcaaca tgaaggtggc gataccttta tggggcatca ggaaatttta ggcacgcgcc
     1381 cgttaccgcc gctgcggatg ccttttcgcg atgtgattga ccgtgttgag caggcattag
     1441 tttccgctgg ctggcaggtg gagcgccgtg gcgatgatct gcaatttctg tcggtcaatc
     1501 aggcggttgc gattggcgat aatctcgagg cggatttagg ccaagtctat aacattaccg
     1561 ccaatctctc tgtgatctct tttgacgacg caatcaaaat tggtcgtatc gtgcgtgacc
     1621 aggtacaggt cggtcgggtc attacatttg gcggcctgtt aaccaacagt cagcgtattc
     1681 tcgatgccgc agaaagcaaa gaagggcgct ttattggtat taatgcgccg cgttctggcg
     1741 cttatgacaa cggcttccag gtcgtgcata tgggctacgg tgttgatgaa aaagtgcagg
     1801 tgccgcaaaa actgtatgaa gcgggcgtgc caaccgtgct ggtgggtaag gtggcagata
     1861 tcgtcagcaa tccttatggt gtgagctggc aaaacctggt ggatagccag cggattatgg
     1921 atatcaccct cgacgaattt aacacccatc cgacggcgtt tatttgcatc aatatccagg
     1981 aaaccgacct cgctggtcat gcagaagacg tcgcacgtta tgccgaacgt ttgcaggtcg
     2041 ttgaccgtaa ccttgcccgg cttgttgagg cgatgcagcc agatgattgc ctggtcgtga
     2101 tggcggatca cggtaacgat ccgaccattg gtcacagcca ccatacccgt gaagtggtgc
     2161 cagtgctggt ttatcagcaa gggctggtcg ccacgcagct cggtgtgcgc accacgcttt
     2221 ctgatgtggg agctaccgtg tgtgaatttt tccgtgcgcc accgccacaa aatggtcgct
     2281 cttttctttc ctccctccgg tttgcaggag acaccctatg agttttgatc cgacgggtta
     2341 caccctggcc catgagcatc tgcatattga tctctccggc tttaaaaaca acgtggactg
     2401 ccgccttgat cagtatgcgt tcatttgcca ggagatgaac gacctgatga cccggggtgt
     2461 acgtaatgtg attgagatga ccaaccgtta catggggcgc aatgcgcaat ttatgctcga
     2521 tgtaatgcgc gaaaccggga tcaacgtggt ggcctgtact ggttattacc aggatgcgtt
     2581 tttcccggaa catgtggtga cccgcagcgt gcaggaactg gcgcaggaga tggtcgatga
     2641 aattgaacag ggtatcgatg gcacggagct gaaagccggg atcatcgcgg agatcggctc
     2701 cagcgaagga aagattacgc cgctggaaga gaaggtattt attgccgctg cgctggcgca
     2761 taaccagacc ggacgcccga tctccacgca tacgtcgttc agcaccatgg ggctggaaca
     2821 actggctctg ttgcaagccc acggagttga tctttcgcgc gtcaccgttg gtcactgcga
     2881 tctgaaagac aacctcgaca acattttgaa gatgatcgat ctcggcgcgt acgtgcagtt
     2941 cgacaccatt ggtaagaaca gttactaccc ggacgaaaag cgcattgcga tgctccacgc
     3001 gctacgtgac cgtgggttgc tgaaccgcgt catgctgtcg atggatatta cgcgccgctc
     3061 ccatttaaaa gccaatggtg gttatggcta tgactattta ttgaccacct ttattccgca
     3121 attgcgccag tcaggattca gtcaggccga tgtggatgtg atgttacgtg aaaatccctc
     3181 tcaatttttc caataaggac agactcatga aaaagattgg cgttgcaggc ttacagcgtg
     3241 agcagattaa aaaaaccatt gaagcgacgg ctcctggctg ttttgaagtt ttcattcaca
     3301 acgacatgga agcggcaatg aaggtgaaat ccgggcaact agattattac attggcgcgt
     3361 gtaataccgg tgcgggcgcg gcattgtcga ttgccatcgc ggtgattggc tataacaaaa
     3421 gttgcaccat tgccaaacca ggcattaaag cgaaagacga gcatatcgcc aaaatgatcg
     3481 ctgaagggaa agtggcgttt ggcctttccg ttgagcacgt cgaacacgcg attccgatgt
     3541 tgattaacca tctgaaataa aaggcacgac tatggatctg tatattcaga ttatcgtggt
     3601 ggcgtgcctg acgggtatga catcgcttct ggcgcatcgc tcggcggctg tttttcatga
     3661 cggcatccac ccgatcctgc cgcaactgat tgaaggctat atgaaccgtc gcgaggcggg
     3721 gagtatcgct tttggtctga gcattggttt tgtggcctcg gtggggatct cttttaccct
     3781 gaaaaccggg ctgctcaacg catggttact ctttcttcct accgatatcc tcggcgtact
     3841 ggcgataaac agcctgatgg cgtttggtct tggcgctatc tggggcgtgt tgatccttac
     3901 ttgcctgttg ccagttaacc agctgctgac cgcgctgccg gtggatgtat taggtagcct
     3961 cggggaatta agctcgccgg tggtttctgc ttttgcactg ttcccgctgg tggcgatttt
     4021 ctaccagttt ggctggaagc aaagtctggt cgccgccgtg gttgttctga tgacccgtgt
     4081 ggtagtcgtg cgctatttcc cacatcttaa ccctgaatcc atcgaaatct ttattggcat
     4141 ggtgatgctg ctggggatcg cgataactca cgacctgcgt catcgtgatg aaaatgacat
     4201 cgatgccagc gggctttcgg tgtttgaaga gcgcacgtcg cggattatca aaaacttacc
     4261 ttatatcgcc atcgtgggag cattgattgc cgccgttgcc agtatgaaga tttttgctgg
     4321 tagcgaagtt tcgatcttca cactggagaa agcgtattcc gcaggcgtga cgccggaaca
     4381 atcgcaaacg ctgattaatc aggcggctct ggcagaattt atgcgcggac tggggtttgt
     4441 gccgttgatt gccaccaccg ccttagcaac gggtgtgtat gcagttgcgg gctttacctt
     4501 tgtttatgcg gtgggctatc tctcgccgaa tccgatggtt gcagcggtat taggcgcagt
     4561 ggttatttcg gcggaagtct tgctgcttcg ttcgatcggc aaatggctgg gacgctaccc
     4621 gtcggtgcgt aatgcgtcgg ataacatccg taacgccatg aatatgctga tggaagtggc
     4681 gctgctggtc ggttcgattt tcgcagcaat taagatggcg ggttataccg gattctctat
     4741 cgcggttgcc atttacttcc tcaacgaatc cctgggccgt ccggtacaga aaatggcggc
     4801 accggtcgtg gcagtaatga tcaccggtat tctgctgaat gttctttact ggcttggcct
     4861 gttcgttccg gcttaaggaa ggcacctatg aagacgtttc ctttgcaaag cctgactctt
     4921 gctgaagcgc agcaaaagca gtttgcgctg gtggacacta tttgccgcca ttttccaggc
     4981 agcgagtttc ttgctggcgg tgatttaggg ttaactccaa gtctgaatca accgcgtatt
     5041 acccaacggg tggagcaggt gctggctgat gcatttcacg cacaggccgc ggcgctggtg
     5101 cagggcgcgg ggactggcgc gattcgcgcc gggctggcgg ctttgctcaa accggggcag
     5161 cgtcttctgg tgcatgacgc gcctgtttac ccgacgacac gggttattat tgagcagatg
     5221 gggctgacgc ttattactgc tgatttcaat gacctgtcgg cactgaagca ggtcgtcgac
     5281 gagcaacaac ctgatgccgc gctggtgcag catacgcgcc agcagccgca ggacagctat
     5341 gtgctggcag atgtgctggc aacgttgcgc gcggcaggtg ttccagcgtt aaccgatgat
     5401 aactatgcgg tgatgaaggt agcgcgaatc ggctgtgaat gcggcgcgaa tgtctcgaca
     5461 ttctcctgct tcaagctgtt tgggccggag ggcgttggtg cagtggttgg cgatgccgat
     5521 gttattagtc ggattcgcgc cacgctttac tccggcggta gccagatcca gggctcacag
     5581 gcgctggaag tcttgcgtgg tctggtgttc gcgccagtga tgcatgcggt gcaggcggga
     5641 gtatccgaac ggttgctggc tttgcttaac ggcggcgcgg tggcggaagt gaaaagcgcc
     5701 gtcattgcca atgcgcagtc gaaggtgttg attgtcgagt ttcatcagct gattgccgcc
     5761 agggtgctgg aagaagcgca aaagcgtggc gcgttgcctt acccggtcgg tgcagagtcg
     5821 aaatatgaaa tcccgccgct cttctatcga ctttcaggaa cgtttcgcca ggcgaatcca
     5881 caactcgaac attgtgcgat tcgcattaac ccgaatcgca gcggtgaaga gacggtgctg
     5941 cggattttgc gtgagagtat tgcgagtatt taatgctgga tagcaccgtt cggcaatcaa
     6001 gctaaattcc gcactgaac
//
LOCUS       DAZXUK010000227         5953 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZXUK010000227 DAZXUK010000000
VERSION     DAZXUK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.227"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            105..386
                     /locus_tag="ACH3L8_003379"
     CDS             105..386
                     /locus_tag="ACH3L8_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086354.1"
                     /translation="MQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIPEVCN
                     LLFTPTIYLLLNIFMLTLGALMLFFSGRVGNVPEFCYVGYDGVGFAVIS"
     gene            complement(592..2097)
                     /gene="glpD"
                     /locus_tag="ACH3L8_003380"
     CDS             complement(592..2097)
                     /gene="glpD"
                     /locus_tag="ACH3L8_003380"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFT4086355.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            2287..2613
                     /gene="glpE"
                     /locus_tag="ACH3L8_003381"
     CDS             2287..2613
                     /gene="glpE"
                     /locus_tag="ACH3L8_003381"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFT4086356.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            2658..3488
                     /gene="glpG"
                     /locus_tag="ACH3L8_003382"
     CDS             2658..3488
                     /gene="glpG"
                     /locus_tag="ACH3L8_003382"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFT4086357.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            3505..4263
                     /locus_tag="ACH3L8_003383"
     CDS             3505..4263
                     /locus_tag="ACH3L8_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFT4086358.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(4245..5843)
                     /gene="rtcR"
                     /locus_tag="ACH3L8_003384"
     CDS             complement(4245..5843)
                     /gene="rtcR"
                     /locus_tag="ACH3L8_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFT4086359.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
BASE COUNT         1345 a         1550 c         1596 g         1462 t
ORIGIN      
        1 ccaaagggtc ccgttttttt cgccatcatt tcggaaaagt gtccagaagg ggcaagggga
       61 ctcggggagt ataatttcgc cattcagaaa gaatggaaag aataatgcaa aatagaaaat
      121 ggattttgac ctcgctggta atgacttttt tcggcatccc aatactgacg caattttttg
      181 cagcggttat tgccatgctg ggggctgggc ttgccgctat tcctgaggtt tgcaatttac
      241 tctttacgcc aacaatttac cttctgctca atatttttat gctgacgctg ggcgcattaa
      301 tgctattttt ctcggggcga gtggggaacg tgcctgagtt ttgttacgtt gggtatgatg
      361 gcgtgggctt tgcggtaatt tcctgaatta aaaagtaggt tagatttttg acaaagtgcg
      421 ctttgttcat gccggatgcg acgtgaacgt cttatctggc ctacaagacc atgcaaattc
      481 aatatattgc ataaaccacg taggcctgat aagcgtagcg catcaggcag ttttgtgttt
      541 gtcatcagtc tcaggccaga ttgaaatctg acctgatcac cttacgttaa tttacgacgc
      601 cagcgacaac ttctgctgcg tatattccac cagccactga ctcacacgag attgttgatc
      661 cgcattcagc cacatgcctt gttttgtgcg acgccacagg gcatcgtcgg tgcggcggac
      721 ccattcgtga tccaccaggt atttcagttc cgcttcgtag aactcatgac cgaaatcttc
      781 cccgagatcg cttaccgttc ccgcattgcc gagcagcagc tcgctgttgc tgccgtaagt
      841 gcgcgtgtag tgacgtgcca gcgattcagt caggaacggg taacggcggc gcaggcgagc
      901 ggcgtaatcg tcgcggtcgc cttcaatggc accacccggc agcacgctct ctttcgtcca
      961 cgccgggcca ataccctgat aatacggcgt cagtttttcc agcgcatgtt ctgccagttt
     1021 tcggtaggtg gtcagcttac cgccgaatac cgacagcagc ggtgctttgc cgttttcatc
     1081 atggatatca agggtgtaat cacgggtaat ggcttgcggc gagtcggact catcatcaca
     1141 cagcggacgc acaccggagt aggtccagac gatatcgtca cggcttagct gctttttaaa
     1201 gtgcgcgtta tacactttca gcaggtaatt gatttcactc tcttcaatct tcactgcttt
     1261 cggatcacct ttgtattcca catcggtggt gccgatgatg gaaaactcgt ccatccacgg
     1321 gatcacgaac acaatgcgtt tatcttcgtt ttgcagaatg taggcctgct tttgggtatg
     1381 cacgcgcggc accacaatat ggctgccttt gatcaggcga atgccataag gcgaaggcag
     1441 atgcatcccc tcgtcgaaga actgtttcac ccacgggccg gtggcgttaa ccaggccgcg
     1501 cgcttgccag ctatattttt tgccggtatc gatatcttcc gcttccacaa tccacaggcc
     1561 gttttcgcgg cgagcagagg tggcgcgagt ccgagtaagc acttcgccgc ctttacgcac
     1621 caccatctgg gcgttggcga gtaccagacg ggcgtcgtct acccaacagt cagaatattc
     1681 gaatccgcgc ttaatttccg gttttaacac tgaatttgcg ccaaaacgca aaccagttga
     1741 tcccggcagg ctggtgcgtt tacccagatg atcgtacata aacagaccaa tgcgaatcat
     1801 ccacgccggg cgcagatgcg gacgatgtgg caggcgaaaa cgcatcggga aggcgatatg
     1861 cggagccatt ttcagcagca cttcacgttc agccagcgcc tcgctgacca ggcggaattc
     1921 atagtgctca aggtagcgca ggccaccgtg aatgagtttt gaactggcgg aagaggtcgc
     1981 gcaagcgaga tcctgcgcct ccagcatcag cacggataaa ccgcgtccag cggcgtctgc
     2041 cgcgatacca gcaccattga tgccgccacc tatcacaatc agatctttgg tttccatgct
     2101 gccctcattc actttcgtta aagctcataa atgttcgtta tcgaacatat tagcaaagaa
     2161 tcgcgctttg ggtaacattg aaaaaacatt ttagagtgat atgtataaca ttatggcgtt
     2221 tatctgccgc ttcgacgtaa actgtgcggt aaatttgccc acttgtttgt aaagaaagag
     2281 agacgcatgg atcagttcga atgtattaac gttgccgacg cgcaccagaa gttgcaggaa
     2341 aaagaggcgg tgctggtcga tattcgcgat ccacagagtt tcgctatggg gcatgcggtg
     2401 caggctttcc atttaaccaa cgacacgctg ggcgcgttta tgcgtgataa cgactttgac
     2461 actccggtga tggtgatgtg ttatcacggc aatagcagca aaggggcggc gcagtatctg
     2521 ctgcaacagg gctacgatgt ggtctatagc attgacggcg gctttgaagc ctggcagcga
     2581 cagtttcccg cagaggtggc gtacggcgcg taacgcttta tactgtcccc ttttgtgtgg
     2641 aataagcgac agcaacgatg ttgatgatta cctcttttgc taacccccgc gtggcgcagg
     2701 cgtttgttga ttacatggcg acgcagggtg ttatcctcac gattcaacaa cataaccaaa
     2761 gcgatgtctg gctggcggat gagtcccagg ccgagcgcgt gcgggcggag ctggcgcgtt
     2821 ttctcgaaaa cccggcagat ccgcgttatc tggcggccag ctggctggca ggccataccg
     2881 gcagtggcct gcattatcgc cgttatcctt tctttgctgc cttgcgtgaa cgcgcaggtc
     2941 cggtgacctg ggtgatgatg atcgcctgcg tggtggtgtt tattgccatg caaattctcg
     3001 gcgatcagga agtgatgtta tggctggcct ggccattcga tccaacactg aaatttgagt
     3061 tctggcgtta cttcacccac gcgttaatgc acttctcgct gatgcatatc ctctttaacc
     3121 tgctttggtg gtggtatctc ggcggcgcgg tggaaaaacg cctcggtagc ggtaagctaa
     3181 ttgtcattac gcttatcagc gccctgttaa gcggctatat gcagcaaaaa ttcagcgggc
     3241 cgtggtttgg cgggctttct ggcgtggtgt atgcgctgat gggctacgtc tggctacgtg
     3301 gcgaacgcga tccgcaaagt ggcatttacc tgcaacgtgg gttaattatc tttgcgctga
     3361 tctggattgt cgccggatgg tttgatttgt ttgggatgtc gatggcgaac ggagcacaca
     3421 tcgccggatt agccgtgggt ttagcgatgg cttttgttga ttcgctcaat gcgcgaaaac
     3481 gaaaataatt ccagggattt ataaatgaaa caaacacaac gtcacaacgg tattatcgaa
     3541 ctggttaaac agcagggtta tgtcagtacc gaggaactgg tggagcactt ctccgtcagc
     3601 ccgcagacta ttcgccgcga cctcaacgag ctggcagagc aaaacctgat cctgcgccat
     3661 catggcggcg cggcgctgcc ttccagttcg gttaacacgc cgtggcacga tcgtaaagcc
     3721 acccagaccg aagaaaaaga gcgcatcgcc cgcaaagtgg cggagcaaat ccccaatggc
     3781 tcgacgctgt ttatcgatat cggcaccacg ccggaagcgg tggcgcacgc gctgctcaat
     3841 cacagcaatt tgcgcattgt caccaacaat ctcaacgttg ctaacacgtt gatggtaaaa
     3901 gaagatttcc gcattattct cgccggtggc gaattacgta gccgcgatgg cgggatcatt
     3961 ggcgaagcga cgctcgattt tatctcccag ttccgccttg atttcggcat tctggggatc
     4021 agcggcatcg acagcgacgg ctcgctgctg gagttcgatt accacgaagt tcgtaccaag
     4081 cgcgccatta ttgagaactc gcgccacgtt atgctggtgg ttgaccattc aaaatttggc
     4141 cgtaatgcga tggtcaatat ggggagcatc agcatggttg atgccgtcta caccgacgcc
     4201 ccgccgccag taagcgtgat gcaggtgctg acggaccacc atattcaact ggagctgtgc
     4261 tgatcctgca cggcttccca cgtcagacca aaacgcgcca ggtatttgcg tagccgatcc
     4321 gcgtcattga cgctggcttt gccctggcgc gaaacatcaa aaagctgtcg tccggcagca
     4381 gaaagcgact ttgcctggcg gcagatagcg ataacgtgtt ccagttgcat acggtcgaag
     4441 agatcgatgt tttctgcctc tgcgcccagc aacgccgtaa gcgcggaggg gcggctctcc
     4501 tgccagttat agcgcagacg gtttatctca tcttccacca cgtccagagt gatgcgtccg
     4561 ctagtggcaa aggtggccat ccgcgtgacg ctggcagaaa gttcgcgaaa gttaccgcgc
     4621 catgttgcct ggggagatgt tgcaaaggcc agccaggcac gccgcgcttc ggtgttaaaa
     4681 cgcacgctgt caccagtgag tgtggcgtgg cgctccactt cataatccag gttcggttca
     4741 atatcttcct ggcgctggcg tagacccggc agggtgaagg tccagagatt gatccgcgcg
     4801 tacagatctt cgcggaattt gccttcggca accagctggc gcaaatcgcg caccgttccg
     4861 gcgataagct gaaaatcact gctcacctgg cgatcgctgc caaacgggta aaaggttttc
     4921 tcttcaatag ctttcagcag cattgcctgt tcgtctgcgc ccagttcgcc aatctcatca
     4981 agaaacaaca ttccgccgtt ggcgctgcgt aataatcctt cacgagattc ccgcgccccg
     5041 gtaaacgcgc ctttcacatg accaaacagc gtcgacatcg cggtatcgcc gcgcagggtg
     5101 gcgcagttca cttccacaaa cgcgccgcta aactgatgcc gtgcctgttt taactcgaag
     5161 atgcgtcgcg ccagaaatga cttacctgcg ccggtcgggc cgttaagcag aatcggcgcg
     5221 cgggatttaa tcgccacttt ttcgatctgc tcaatcatgc ggttgaagtg ggggttacgc
     5281 gtggcaatac cggatttgag gaaatcgagc gtttgctgcc gttcctcggc aaagcggctg
     5341 gcgatggcgt tataacggct taaatcgaga tcgataatcg tcacttcacc tgggccgcgc
     5401 ggctgttctt ttttgcgcgg agttgaagat tgtatcaacc gggcgggtag ataacgcgct
     5461 tccgccagca gaaaccagca aatctgcgcg acgtgggtgc cggtggtgat gtgaattaaa
     5521 tagtcttctt tttctggctg aaactcgtaa ccacgggcga aatcatgcag gcaggcgtaa
     5581 acctcttcga aatcccacgg gttatgcagt tcaatctcaa cgcccaccac ttctgtttct
     5641 ggcgaaacgc tggcgatatc gcgtttaagc gtttcaaata gcgagcgcga gcgggcatcg
     5701 tgcaataatt ccagtcgatc gatgaccaac gattcttgct ggtatataca gagtgacgga
     5761 cgccattttg accagcgctg actgccgcgt ccggcataat ccagcacggt accgacaaag
     5821 ccaaaagcca ctgttttacg catcttagat atccttataa aaggcgatat ttcattatcg
     5881 gtaaatcggc tttgcgggaa atctccgttt acggatataa agatataatc tttttagaaa
     5941 caataaatta atc
//
LOCUS       DAZXUK010000228         5889 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZXUK010000228 DAZXUK010000000
VERSION     DAZXUK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.228"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..244
                     /locus_tag="ACH3L8_003385"
     CDS             <1..244
                     /locus_tag="ACH3L8_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086360.1"
                     /translation="EMLSFLTSEEREALTNMLARKGVETVLRLLDKETQELLQLEGRK
                     RLAALMLVDMPDEQVREILEEIEASILSESVNAKAG"
     gene            493..1437
                     /gene="tauA"
                     /locus_tag="ACH3L8_003386"
                     /pseudo
     CDS             493..1437
                     /gene="tauA"
                     /locus_tag="ACH3L8_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="ACH3L8_003387"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="ACH3L8_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFT4086361.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="ACH3L8_003388"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="ACH3L8_003388"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFT4086362.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="ACH3L8_003389"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="ACH3L8_003389"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFT4086363.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACH3L8_003390"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACH3L8_003390"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFT4086364.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5493..5861
                     /locus_tag="ACH3L8_003391"
                     /pseudo
     CDS             5493..5861
                     /locus_tag="ACH3L8_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1399 a         1454 c         1598 g         1438 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaatatt tttaatcttt atttatagct cttggttatt
     5161 attttttggc gtttggttgt gctgctataa attgaataat tcaaactttg ttgtatattg
     5221 ataagatatt tatctggttt gtatttttga tatttataac gcgtatgcat caaaaataac
     5281 attatctgca acatactgaa atatcgtcct tttatcaatt tgatgcgtgt ttttctccat
     5341 aaattatata tgtccacatt cggacttagg ggaaagaata attgaaccat tcgtctgtaa
     5401 cgcagcataa tcgttagcgc gaaacataat atgttttcta tgcattgata attgatggat
     5461 caacttatta cgtccctgag gagggatgac aaatgcactc ctggaaaaag aaacttgtag
     5521 tatcacaatt agcattggct tgcactctgg ctatcacctc tcaggctaat gcagcgacca
     5581 acgatatttc tggtcaaact tacaatactt tccatcacta caacgacgcc acctatgctg
     5641 atgacgttta ctatgatggt tatgtaggct ggaacaacta tgccgctgat agctattaca
     5701 acggcgatat ctacccggtc attaataacg ctaccgttaa cggcgtgatt tctacctact
     5761 atctggacga cggtatttct accaatacca acgccaatag tctgacaatc aaaaacagca
     5821 ctattcacgg tatgatttat tccgagtgca tgacaactga ttagactggc cccctgaatc
     5881 tccagacaa
//
LOCUS       DAZXUK010000229         5880 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZXUK010000229 DAZXUK010000000
VERSION     DAZXUK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.229"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..609
                     /gene="ruvA"
                     /locus_tag="ACH3L8_003392"
     CDS             <1..609
                     /gene="ruvA"
                     /locus_tag="ACH3L8_003392"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFT4086365.1"
                     /translation="IGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQEA
                     IVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAVE
                     REEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAEQ
                     EAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            618..1628
                     /gene="ruvB"
                     /locus_tag="ACH3L8_003393"
     CDS             618..1628
                     /gene="ruvB"
                     /locus_tag="ACH3L8_003393"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFT4086366.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1775..2560)
                     /gene="znuB"
                     /locus_tag="ACH3L8_003394"
     CDS             complement(1775..2560)
                     /gene="znuB"
                     /locus_tag="ACH3L8_003394"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFT4086367.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2557..3312)
                     /gene="znuC"
                     /locus_tag="ACH3L8_003395"
     CDS             complement(2557..3312)
                     /gene="znuC"
                     /locus_tag="ACH3L8_003395"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFT4086368.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3391..4323
                     /gene="znuA"
                     /locus_tag="ACH3L8_003396"
     CDS             3391..4323
                     /gene="znuA"
                     /locus_tag="ACH3L8_003396"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFT4086369.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4339..5661
                     /gene="mepM"
                     /locus_tag="ACH3L8_003397"
     CDS             4339..5661
                     /gene="mepM"
                     /locus_tag="ACH3L8_003397"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFT4086370.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5781..>5880
                     /locus_tag="ACH3L8_003398"
     CDS             5781..>5880
                     /locus_tag="ACH3L8_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310592.1"
                     /note="Transfers myristate or laurate, activated on ACP,
                     to the lipid IVA moiety of (KDO)2-(lauroyl)-lipid IVA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFT4086371.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAM"
BASE COUNT         1556 a         1474 c         1515 g         1335 t
ORIGIN      
        1 ataggcagac tcagaggcat catcattgaa aaacaacccc cgctggtgtt aattgaagtg
       61 ggcggcgtag gctatgaagt gcatatgccg atgacctgtt tttatgaact ccctgaagcg
      121 ggtcaggaag cgatcgtttt cacccacttt gtggtgcgtg aagacgcgca actgctgtac
      181 ggttttaaca ataaacaaga acgcacgttg ttcaaagagt tgatcaaaac caacggcgtc
      241 ggcccgaagt tggccctggc gatcctctcc ggaatgtcag cgcagcagtt cgttaatgcc
      301 gttgagcgtg aagaagtggg ggcactggtg aaactgccgg gtattggcaa aaaaaccgcc
      361 gaacgcttga ttgttgaaat gaaagaccga tttaaaggtt tgcatggcga tctctttacg
      421 ccagccgccg acctggtact cacgtctcct gctagcccgg cgaccgacga tgctgaacaa
      481 gaagcagttg ccgcgctggt ggcgctaggc tataaaccac aagaagcaag ccgcatggtg
      541 agcaaaatcg ctcgccctga caccagcagt gaaacattaa ttcgcgaagc cctacgcgcc
      601 gcgttatgag gtaaaggatg attgaagcag accgtctgat ttctgccggt accactttgc
      661 cggaagatgt agcagatcgc gccattcgcc ctaaattact ggaagagtat gttggtcagc
      721 cgcaggttcg ttcacagatg gagattttca tcaaagcagc gaaactgcgc ggcgatgccc
      781 tcgatcattt gttgattttt ggtcctccgg ggttgggtaa aactacgctt gccaacattg
      841 tcgccaatga aatgggcgtt aatttacgca cgacttctgg tccggtgctg gaaaaggcgg
      901 gcgatttggc tgcgatgctc actaaccttg aaccgcatga cgtgctgttt attgatgaga
      961 tccaccgttt atcgccagtt gttgaagaag tgctgtaccc ggcaatggaa gactaccaac
     1021 tggatatcat gattggtgaa ggtccggcgg cacgctccat taaaattgat ttgccgccgt
     1081 ttaccctgat tggcgcaacc acgcgcgcag gttcgctgac atcaccgttg cgcgaccgtt
     1141 ttggtattgt gcaacgtctg gagttttatc aggtgccgga tctgcaatat atcgtcagtc
     1201 gcagtgcacg ctttatgggg cttgagatga gtgatgacgg tgcgcaggaa gtagctcgtc
     1261 gcgctcgcgg tacgccacgt attgccaacc gtctgctgcg tcgagtgcgt gatttcgccg
     1321 aagtgaagca cgatggcacc atctcggcag atatcgctgc tcaggcgctg gatatgttga
     1381 atgtcgatgc tgaaggtttc gattatatgg accgcaaatt gttgctggcg gtaatcgata
     1441 agttctttgg tgggccggta ggtctggata acctggcggc agccattggc gaagaacgtg
     1501 aaaccattga agatgtactg gaaccttatt tgattcagca aggctttttg cagcgtacac
     1561 cgcgcgggcg tatggcgacg acgcgggcgt ggaatcactt tggcataacg ccgccagaaa
     1621 tgccgtaagt cggattggtc gctcgcgccg catccgacac tggcagtgtg tctgatgcga
     1681 cactgttcgc gtcttatcag gccagccaga gatagcgaac cgtaggtcgg ataaggcgct
     1741 cgcgccgcat ccgacaaatg tgttcagcga tagattagct ggcctgcttt ttcatcatac
     1801 tgataataaa taacagtgcc gcacatagaa ccaccgacgg acccgccggc gtatcgtaaa
     1861 acgcggaaaa ggttaaaccg ccagttactg ccaccatccc caccaaaaca gcgacaccag
     1921 ccatctgttc cggcgtacgg gcaaagcgac gcgcagtagc tgcaggaata atcagcaacg
     1981 aagtaataat caacgcaccg acaaatttca tcgctacacc aatcgtcaat gccgtcacca
     2041 gcatcaacaa caatttcact tgctgtaatt tcacaccatc aacaaacgcc agatccgggc
     2101 taatcgtcat cgacagcaaa ttgcgccatt gccagaacaa aatagccacc acgatgacca
     2161 cgccaatcgc aatagagatg agatcttctg gcgtcactgc cagtaaatca ccgaacaggt
     2221 aagccatcaa atcaacacga atgttagaca tcagactaac gaccactagg cccagcgaca
     2281 gggcactgtg cgccataatc cctaataacg tgtcgatcgc cagctgtgga cgcttctcca
     2341 gccataccag accgcccgcc agcagcagcg taacggcaat caccgcatag aatggattca
     2401 cgtccagcaa caaaccaaac gcgacaccaa gtaatgaggc atgagccagc gtatcaccga
     2461 aataagacat acgacgccag actacaaacg aacccagcgg acccgcggca caggcgagca
     2521 tgatcccggc taaccaaccg ggaaataata attcaatcat gagcgatcat ttccccgacg
     2581 caaaacaatt cgtccctgta aatcgtgacg atgattatga tgatggcgat agatacccag
     2641 ttgttcagca ccacgaggac caaacattga aataaactcc ggatgcaggg aaacaacttc
     2701 cggtgtgccg gaacaacaaa tgtggtgatt caggcacagc acttcatcgg tttttgccat
     2761 caccagatgc agatcgtgag aaaccattaa aacgccacaa tccagttcac gacgcaactg
     2821 gtcaataagg tcatataacg ccacttggcc atttacatcc acgccttgag tgggttcatc
     2881 cagcactaat aattgcgggc aatttaacaa tgctcgcgct aatagtacac gctgcgtttc
     2941 gccaccagag agcttttgca tcggtgcgtt aatcaggtgc ccggcctgga cacgtttcag
     3001 tgcaggcaaa atatcttctt tatgtgtacc agggcgtaag cgtaaaaaac ggtttacggt
     3061 cagtggcaac gtggtgtcga gatacagctt ctgcggtaca tagccgatgc gcagttttcc
     3121 gttgcgcttg ataacccctt catcgggtgt taccagcccg agcactaccc gtaccagtgt
     3181 cgacttacct gcgccatttg gcccaagtaa agtcaaaatt tttccaggtt taagttccag
     3241 cgacacatca gagaggacgc ggcgttggcc aaaagaaacc gagacatttt ccagggaaac
     3301 cagacttgtc atgttaattt tagtcttgca gtagtcatga aatgttataa tatcacactt
     3361 ctcatattca ttacgatgat tggtcgcatt atgttacata aaaaaacgct tcttttcgca
     3421 gcattatccg ccgctctctg gggaggtgca acacaggccg cagatgccgc cgttgtcgct
     3481 tcgcttaaac ccgttgggtt catcgcttct gccattgctg atggtgtaac agaaacggag
     3541 gttttactgc ctgacggcgc ttcagaacat gattattcac tgcgtccatc ggatgtaaaa
     3601 cgcttacaga acgcggactt agtcgtttgg gttggcccgg agatggaagc gtttatgcaa
     3661 aaaccggtaa gcaaattacc agaagcgaag caggtaacga ttgcgcagct tgaagatgtg
     3721 aaaccgctac tgatgaaaag tattcacggc gatgatgatg atcacgacca cgcggaaaaa
     3781 agtgacgaag atcaccatca cggcgatttc aacatgcatc tttggctttc cccagagata
     3841 gcgcgggcta cagcggttgc aatccatgga aaattagtgg aacttatgcc gcaaagtcga
     3901 gccaaacttg acgccaacct gaaggatttt gaggcacaat tggcctcaac cgaaacgcag
     3961 gttggtaacg agctcgcgcc gctcaagggg aaaggttatt tcgtttttca cgatgcttac
     4021 ggctatttcg aaaaacagtt cggactgaca ccgcttggtc attttaccgt taaccctgag
     4081 attcaacctg gcgcgcagcg tttacatgaa ataagaacac agttggttga gcaaaaagca
     4141 acctgcgttt ttgctgagcc acagttcagg ccagcggtcg ttgagagcgt cgcacgaggg
     4201 acatccgttc gtatgggaac gttggatccc ctggggacga atatcaaact gggtaaaaca
     4261 agctattcag aattcctgag tcaattagcc aaccagtatg cgagctgcct gaaaggagat
     4321 taatgaggaa gtgattacgt gcaacagata gcccgctctg tcgccctggc gtttaataat
     4381 ttaccgcgac cacaccgcgt tatgttgggg tcgctcaccg ttcttactct ggccgtcgct
     4441 gtctggcggc cctatgttta tcaccgtgat gccacgccaa ttgtcaaaac cattgagctg
     4501 gaacagaacg aaattcgttc gctcttacct gaagccagtg agccgattga tcaagctgca
     4561 caagaagatg aagccattcc ccaggacgaa ctggatgaca aaatcgccgg tgaagcgggc
     4621 gtgcatgaat atgttgtttc cactggcgat acgctaagca gcattctcaa tcagtatggt
     4681 attgatatgg gtgatatcac ccaacttgct gctgccgaca aagaattgcg taacctgaaa
     4741 atcggtcaac aactctcctg gacattaacc gcggacggcg aactgcagcg cctcacctgg
     4801 gaagtgtctc gtcgtgaaac ccgaacctat gaccgtactg ccgctaacgg ttttaaaatg
     4861 accagcgaaa tgcagcaagg agagtgggtt aacaatctgc tgaaaggtac cgtcggaggg
     4921 agctttgttg ccagcgccag aaacgccggt ttaaccagcg ccgaagtgag cgcagtgatt
     4981 aaagccatgc agtggcaaat ggatttccgc aaactgaaaa aaggcgatga atttgcggta
     5041 ttaatgtctc gagaaatgct tgatggtaaa cgtgagcaaa gccagctgct gggcgtacgt
     5101 ttgcgttcag aaggtaaaga ttattacgca atccgcgctg aggatggcaa attctacgat
     5161 cgtaacggta ctggtctggc gaaaggattc ttgcgattcc cgacggcgaa acagttccgt
     5221 atctcgtcta actttaaccc gcgtcgaact aatccggtga caggtcgcgt tgcaccacac
     5281 agaggtgttg atttcgccat gccacaaggt acgccagtgc tttcagtggg tgacggtgaa
     5341 gtggtggttg ccaaacgcag tggcgcagca ggttattatg tggctattcg tcatggtcgc
     5401 agctacacca cgcgttatat gcacttgcgc aagattctgg tgaaaccggg acagaaggtg
     5461 aaacgtggcg accgtatcgc gctttccggt aataccggac gttcaactgg gccgcatctg
     5521 cactatgaag tatggataaa ccagcaggcc gtaaacccgc tgacggcaaa actgccgcgt
     5581 accgaagggc tgaccggctc cgatcgtcgc gaattcctgg cgcaggccaa agagattgtg
     5641 ccgcagctac ggtttgatta attaacatcc attcgcagcc ggtacgcagt cagtaccggc
     5701 tttttttatt tggtgcgggg caagttgtgc cgctacacta tcaccagatt gatttttgcc
     5761 ttatccgaaa ctggaaaagc atggaaacga aaaaaaataa tagcgaatac attcctgagt
     5821 ttgataaatc ctttcgccac ccgcgctact ggggagcatg gctgggtgta gcagcgatgg
//
LOCUS       DAZXUK010000230         5861 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZXUK010000230 DAZXUK010000000
VERSION     DAZXUK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.230"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            35..481
                     /gene="hpaR"
                     /locus_tag="ACH3L8_003399"
     CDS             35..481
                     /gene="hpaR"
                     /locus_tag="ACH3L8_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFT4086372.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            600..2264
                     /gene="tsr"
                     /locus_tag="ACH3L8_003400"
     CDS             600..2264
                     /gene="tsr"
                     /locus_tag="ACH3L8_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFT4086373.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(2313..3674)
                     /locus_tag="ACH3L8_003401"
     CDS             complement(2313..3674)
                     /locus_tag="ACH3L8_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4086374.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(3889..4803)
                     /locus_tag="ACH3L8_003402"
     CDS             complement(3889..4803)
                     /locus_tag="ACH3L8_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFT4086375.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            4942..5861
                     /gene="lgoD"
                     /locus_tag="ACH3L8_003403"
                     /pseudo
     CDS             4942..5861
                     /gene="lgoD"
                     /locus_tag="ACH3L8_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313345.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
BASE COUNT         1520 a         1357 c         1593 g         1391 t
ORIGIN      
        1 tattaatgat tttgaatttt tcaggggaaa gataatgcac gactcactaa ccattgcgtt
       61 gctccaggcg cgagaagcgg cgatgagtta ttttcgccca atcgttaaac ggcataattt
      121 gactgagcaa cagtggcgca tcgtgcgtat tctggcggaa agcccatcaa tggattttca
      181 cgatctggcg tatcgcgcct gcattttgcg cccaagcctg accgggatcc tcacgcggat
      241 ggaacgcgac ggtttagtgt tgcgattaaa gccgatcaat gatcagcgga agctctatat
      301 ctcgctgacc aaagaggggc aggcgctgta taaccgcgct cagacacaga ttgaagaagc
      361 ctatcggcag attgaagctc agtttactgc cgagaaaatg caacagttaa cccatctgtt
      421 agaggaattt attgctctgg gcaattcccg acaagaagac atcccgggcg ataatgaata
      481 actttttagt atctgataac cagcgcctgg tttttccatg gatggcgggt tacacctttt
      541 cataaagttt ttgctttcca ggccgaaaat cttgcatcgg tccacaggaa agagaaacca
      601 tgttaaaacg tatcaagatc gtgaccagct tactgctggt tttggccgtt tttggccttt
      661 tacaactgac atcaggcggt ctgtttttta atgccttaaa gaatgacaaa gaaaatttca
      721 ctgttttaca aaccattcgc cagcagcaac ccacgctgaa tggcagctgg gtcgcgttgt
      781 tgcagacgcg taacaccctc aaccgcgcgg gtatccgcta catgatggat cagaataata
      841 ttggtagcgg ttcaaccgtt gctgagctga tgcagagtgc cagtatttcg ctgaaacagg
      901 cggaaaaaaa ctgggcggat tacgaagcgt tgccgcgtga cccgcgtcag agcaccgccg
      961 cagcggcaga gatcaaacgt aattacgata tttatcacaa tgcgctggcg gagctgatcc
     1021 aactgttagg tgcaggcaaa atcaacgagt tctttgatca gccgacccag ggatatcagg
     1081 acggtttcga gaagcagtat gtggcttaca tggagcaaaa cgatcggctc tatgatatcg
     1141 ccgtcagcga taacaatgcc tcctacagcc aggcgatgtg gattctggtg ggcgtgatga
     1201 tcgtcgtact ggcggtcatc ttcgccgtct ggttcggtat taaagcctcg ctggtagcgc
     1261 caatgaatcg cctgattgac agcattcgtc atattgcagg cggcgatctg gtgaaaccga
     1321 ttgaggtgga tggctctaat gagatggggc aactggcaga gagtttgcgc catatgcagg
     1381 gagagctgat gcgtaccgtc ggtgatgtgc gcaacggggc caatgccatc tatagcggtg
     1441 ccagcgaaat cgctaccggc aataatgatc tctcttcgcg caccgagcaa caggccgctt
     1501 cgctggaaga gacggcagcc agcatggagc aactgaccgc aacggtgaaa cagaacgccg
     1561 agaatgcgcg ccaggccagc catctggcat taagtgcttc tgaaacggcg caacgcggcg
     1621 gcaaagtggt agataacgtg gtgcagacta tgcgcgatat ctccaccagt tcgcagaaaa
     1681 tcgccgatat tatcagcgta attgacggca ttaccttcca gaccaatatt ctggctttga
     1741 acgcggcggt tgaagcagcg cgtgcgggtg agcaaggacg tggttttgcg gtggttgcgg
     1801 gagaagtgcg taatctggcc cagcgtagcg cccaggcggt tcgtgaaatt aaaagcctga
     1861 ttgaagactc ggtggggaaa gtggatgttg gctctacgct ggtcgaaagc gccggggaaa
     1921 caatggcgga gattgtcagt gctgtgaccc gcgtgacgga cattatgggc gaaatagctt
     1981 ctgcttctga tgagcagagc cgtggtatcg atcaggttgg cttagcggtt gctgagatgg
     2041 accgggtaac tcaacagaac gccgcgctgg tggaagaatc tgccgctgcc gccgccgcgc
     2101 tggaagagca ggccagtcgc ctgaccgaag ctgtggcagt gttccggatt cagcaacagc
     2161 agcaacagca gcgtgaaaca tcggctgtgg taaaaaccgt gacgccagct acgccgcgta
     2221 aaatggcagt ggcagatagc ggggagaact gggaaacgtt ttaatcgcca taaaaatgcc
     2281 cgataagcaa aatgttatcg ggcataggga gtttaatctt tacgcgggtc gttgatcggc
     2341 tggcgaacca ggaagatgta cgccagtgct cccgctgcgg tgacgcaacc gcaaatgatt
     2401 agtgccagac ggaatgagtg ggttgtatca acaataaaac cagtaatgat cggcgcgaaa
     2461 gaggcgcaga tgaagctggc aaagttctgg atactgccca ccgacgcagt catgcgagaa
     2521 gcaactgcga cgtggatcaa gccccagcag gatgttccgg caaagtgaat acagaacagt
     2581 gccatgccaa tcagcagaac cgccatcatg gatgttgtcg cttgtggtac tatcagcgta
     2641 aaggcggcag aacagaacat cccggtaata atgcagatct tacggctttt aatcggagcc
     2701 attccccctt tgaccagcca gtcagtaacg taaccgttga ccagcatccc ggcagcccca
     2761 aacaggaaag ggatagccgc catcaaccct gtgcttttta aatccaggtt ataggctgtt
     2821 tgcaggtaac caggaagcca ggccagatac agccacgcag tgtagttgat gccactgaat
     2881 ccgagcatca ttccccacat tgtgcggtta cggaacaggc tgcgccattc ggcaaaattg
     2941 agcggatctc ggcgggcatt gacgctacct gcattgagat aagcttgttc aacggcagtc
     3001 agttctacgt gctcgcggtt gcgatagagc atataccagc cgatggcgag aaaaatcccc
     3061 agtacaccaa tggtaataaa catcccgcgc cagcccatca ccagcatcat tgccgccaga
     3121 atcggtgggc ttacagcaac gccaatggta gaagctgcgt tgaagaagcc catcgggcgt
     3181 ccgcgctctt tgatgttgaa ccagtcgtta atgactttta caccgcatgg gttcatcggc
     3241 gcttcaccaa tccccatacc gatacgcacc aacacgaact gcgtaaagct gtgcaccatg
     3301 ccagacatgg cctggaacag tgaccagaag aacatcccca gtcccagcat caggcgtggg
     3361 cctttacgat ccaatagtgg gccgcaagga agttgcgcaa tgccgtaagc gagtgaaaac
     3421 acggagagca aagcgccgat ttcggtggca cttaatccca attcttcacg aatcgttaaa
     3481 tttgctaccg acagcgaact gcggtcgaga taattgatta ccgccgcaaa aaataataac
     3541 aacatggcgg tggtttgaat gcgtttaatt cgggtactgc gttgaactaa tccatctggt
     3601 ggcacgggaa tatctgccga cgaagatgga gtaaatgaag aacgcggatc gagggtgata
     3661 ttttcttttt ccacgccgga ctcctgtatt tataatttta attgtttacg taattgtgca
     3721 ttacgcaagg ttgacattaa cggcaggtta aagttttagg actttactac cgctcacaaa
     3781 aacattttta gtcgactgat tgtgagcata ggtaagcggg aatttcccgg tatttatctt
     3841 ttgataaaac cgtgcacccg ctaatagttt gctgttttta atccgtactt aatgagcata
     3901 acgcgtgttc tcattaatgg atcgaatcat tgattgttta gctgaattca aatggttacg
     3961 gagcgcaagg gtggcatcaa ggtcactgcg gcaaatcaga gcgctgagaa tggtcatatg
     4021 ttcatcgacg gcaatgatat tgcgctgttt aagatcgctt tcgtcccatt gatagtgaaa
     4081 atgaaagatg actgagatga tctcaagcga ttgatcaaaa aagatattgt cggcagctga
     4141 aagcagtaag gagtgaaaat ctctgtcaag ttgcgagaac atgcgaaagc tattgccgat
     4201 gttgtcacgc agtagccgat gacgttctag catggttttc gcctgcagcc agcggggatc
     4261 atgatcaggc aggttgagaa agtgctgaag tgaatgtgtt tcgagcattt cgcgtagctc
     4321 aaacagctgc tcggcgtagg attgatcgaa ttgcttcatg ctccactggc cgcgcttttc
     4381 gctttgaata agattataac ggccgaattt taaaagatat tctcttacaa ccaccgggct
     4441 gacacccgcg gcccgagcca gttgcagttc agaaaaagtt tccctagggc gtaactgacg
     4501 ctggtttatc atcgtgaaaa aagcctgctc aaacactttg ttttgttcac tcattgaagc
     4561 ggtagtacag gcgaaaccgt catcatggtc aggtttacgc gcaataacat agtcgtttcc
     4621 aacctgcgtt aagacgccgc attcgcgtaa ttggctgaga atatgacgca ctgtagtgcg
     4681 gctgatgttg tacatttccg ccagcgcact ttgcgatgga agtggggaag ggatatgacc
     4741 ccgcgccata tcatcaatga cctggttaat cacattgtgg cgtaaatttt gtgaacgact
     4801 catattttcc tccttgtgag tccattaaaa cccgatttaa aacattttta tgcgtgtgtt
     4861 ttcacaaaac agattttccg ttccgctgcc atttttcatt ttcattgata tctaaggcaa
     4921 caaaaaatgg atagccgaat catgtctacg atgaatgttt taatttgcca gcagccgaaa
     4981 gaattagtct ggaaacaacg cgagatacct attccgggtg acaatgaagc attaataaaa
     5041 attaagtctg tcgggatttg cggtaccgat attcatgcct agggtggaaa tcaaccattt
     5101 tttagttatc cacgggtttt aggccatgaa atatgtgggg agattgttgg gctgggtaaa
     5161 aatattgctg atcttaaaaa tggtcagcaa gttgctgtga tcccttatgt tgcctgtcag
     5221 caatgcccgg cgtgtaaaag cgggcgtacc aactgctgtg aaaaaatttc agtcattggc
     5281 gtgcatcagg atggcggttt tagtgagtat ttgagcgtgc cggtggcgaa cattttgccc
     5341 gcagacggta ttgacccgca ggcggccgca ttgattgaac ctttcgctat tagcgctcat
     5401 gcggtgcgtc gcgcagccat tgctcccggc gagcaggtgc tggtggtcgg ggcggggcca
     5461 atcggtctgg gcgcggcggc aatcgctaaa gccgatggcg cacaggtggt ggtggcggat
     5521 accagtccgg cgcgccgtga acatgtggca acgcgtctgg agttgcccgt gctggacccg
     5581 tcagccgaag attttgacgc gcagctgcgg gcgcagtttg gtggttcgct ggcgcagaaa
     5641 gtgatcgacg cgacaggtaa tcaacatgcg atgaataata ccgtgaatct gattcgtcac
     5701 ggcggcacgg tggtatttgt cggtctgttt aaaggtgagt tgcagttctc cgatccggaa
     5761 ttccataaaa aagaaacgac gatgatgggc agccgcaacg ccacgccgga agatttcgct
     5821 aaagtcggtc gactgatggc ggaagggaaa atcactgccg a
//
LOCUS       DAZXUK010000231         5744 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZXUK010000231 DAZXUK010000000
VERSION     DAZXUK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.231"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..173
                     /locus_tag="ACH3L8_003404"
                     /pseudo
     CDS             1..173
                     /locus_tag="ACH3L8_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(489..827)
                     /locus_tag="ACH3L8_003405"
     CDS             complement(489..827)
                     /locus_tag="ACH3L8_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086376.1"
                     /translation="MVAETMFPNIAGSRSCIDCHHVAGRTFSKSARRIRGAPLLRRTS
                     RYDAQTSAFGISNDFRCISYVIPFWVVSSAMNVIPIPSLQTHYRSFITTTNGSAPVSR
                     YVPETGSHVP"
     gene            749..1876
                     /gene="ltrA"
                     /locus_tag="ACH3L8_003406"
     CDS             749..1876
                     /gene="ltrA"
                     /locus_tag="ACH3L8_003406"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFT4086377.1"
                     /translation="MFDQQRDGNLYKIWNRLCSGTWFPPPVLEKRIPKSNGKERILGI
                     PTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDALKQCAIRCWRYSWILEVD
                     ISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSCENGELITRTRGTPQGGV
                     ISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRMSDATRLKNRLSERFSEV
                     GLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNFNGERYRKCMPGASNAAM
                     RKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRL
                     LKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE"
     gene            1959..2138
                     /locus_tag="ACH3L8_003407"
                     /pseudo
     CDS             1959..2138
                     /locus_tag="ACH3L8_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2165..2650)
                     /locus_tag="ACH3L8_003408"
     CDS             complement(2165..2650)
                     /locus_tag="ACH3L8_003408"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFT4086378.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAAELRQ
                     K"
     gene            complement(2906..3886)
                     /locus_tag="ACH3L8_003409"
     CDS             complement(2906..3886)
                     /locus_tag="ACH3L8_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFT4086379.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3886..5085)
                     /locus_tag="ACH3L8_003410"
     CDS             complement(3886..5085)
                     /locus_tag="ACH3L8_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFT4086380.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5259..5393)
                     /locus_tag="ACH3L8_003411"
                     /pseudo
     CDS             complement(5259..5393)
                     /locus_tag="ACH3L8_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5407..>5744)
                     /locus_tag="ACH3L8_003412"
     CDS             complement(5407..>5744)
                     /locus_tag="ACH3L8_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFT4086381.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1574 a         1317 c         1368 g         1485 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtagagg tgggccctcc ggtcagggtt tacaggagca
      241 cagaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtgggttcg ggaagctgaa cgaaagtaaa ctgtccgatg
      361 aagcatcgaa atcttagatg atgccaaaat ggggccgtac agccgtcccc tgataagcgc
      421 agaagacacc tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc
      481 agaccatctc agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggac
      541 ccattcgtag tagtgatgaa agatctgtaa tgggtctgga gcgaagggat ggggatcaca
      601 ttcattgcag aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt
      661 gaaataccaa aagcactggt ctgggcatca tatctggatg tgcgtcgcaa caagggagcc
      721 ccccggatgc gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata
      781 caagatctgg aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg
      841 gatcccgaag tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat
      901 tgctcaggga gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga
      961 ttcatatggt taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg
     1021 gtgctggcgt tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgag
     1081 acatgatctg gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta
     1141 ctgtcgacgc tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg
     1201 aacacgaggc actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca
     1261 ctatgcattt gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc
     1321 tgacgatatt gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt
     1381 atcggagcgc ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta
     1441 cattgacacg tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt
     1501 caaagtgcgt acgctgaaga atttcaacgg cgaacggtac cgaaaatgca tgccgggtgc
     1561 gtcaaatgca gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc
     1621 aacagctgag agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat
     1681 cgagtactac ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca
     1741 gtcacgtctg ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg
     1801 aaagctgacg ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg
     1861 tgcatcaaat gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag
     1921 cagccgggga gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg
     1981 aacatgcggc ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag
     2041 aaaaatggct gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg
     2101 atctgttgcc ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag
     2161 ccgcttactt ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa
     2221 gaagtccacg aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat
     2281 tcaaaaaagt ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc
     2341 catgttggca ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct
     2401 gataagcttt aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt
     2461 cccgggccca gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt
     2521 gcaccagcca gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat
     2581 gagcattgat actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct
     2641 gttcagacat ggtatcccgc ttacttttgt tgttaaagtt tatccatcat ggcgcaattg
     2701 aaggctgccg tgaaagaggg ggaagactat ctcatcggtt actgttaaac ggctaagaga
     2761 aactatcttt ttcaagcaaa gaagatacgg tgaaacaggc gatttcaccg tgaaatactg
     2821 ttcaaaaaaa acgctaataa aagcaaaatt tcttttaaaa cagccaatta aaaactggcg
     2881 agttgctggc gaaaaacttg aattttcaca gcgaattcag attatctttt agtacaagag
     2941 caatcatacg atctaactta tcctgaactt ccaacggtaa tctgccgaat tcatatgaga
     3001 acgtacgtcc ctttactcgt tttcgagcaa atttatcttt actatcaaat ttccataaca
     3061 aagttacaac cgctttatcc tttactcctg aatcaagcaa tgaagacatt tccttggtta
     3121 taagccgcat aatactgttt ttaacctcgt cttcagaaag attgtcatca gcagtaataa
     3181 gctcaatact cggcgagata ttcttcacaa cttcatcaat agatatatca cccagcctca
     3241 gtcgttcact taattcagcc agttgcctgt aatcggcgta tgtcagctca gactgaacag
     3301 gaaaaagaga cacaaaatct ttaggtacgg acgctgcttg gagggcacga gtcaccttag
     3361 ctgccgacaa tccctctctt tccgcaattt gcttctggtt cataccggcc tctttgagac
     3421 gtactaaacg caacccaatt tcacgaatat tatgttcaag tgacgtctgc aaatctttcg
     3481 ccagatgctg agcttcagaa acagtaagct catcatccgt tacaagtaca cgtaatccaa
     3541 ccttgcatag caaagctgct gcccgacgac gggaaccatc caaaatctca ataagatcgc
     3601 cagtccgaat cccaatacat ggatagaatt gttgtaaacg tatagttctg gtgatgtctt
     3661 ttagtgagtc cggcgtcagg gctgtctgtt cgcggccatt cacctcttca accacaaaag
     3721 tttgagtatc cactttgtca tgatcaacct tgatctcagt aaattttgcc tttcggccag
     3781 tattgagggt aaatacatga actggagccg atatttcttc agtattattc aggatatttg
     3841 tattcagcgt acggccaata gtcggtcggt gctttctgtt ttccatcagt agttggccct
     3901 tataaattca atacgatcaa aaactgcttt agcgaaatct tctgctgcca tacgtgcatt
     3961 tttcaaagct tcaccactgc caacataagt aacaggattc gcagatatca cggtatcaaa
     4021 cgactcgccg cttctctcaa aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc
     4081 accgaaaatc tcttttgtca ggctatggca gtatttatga tctgacttat tcgccagttt
     4141 cgacatgaag ccaatatttg cctgcaaacg gcatgaacat ccatcctgtt caataatctg
     4201 tacaagttca ggtaaacgag ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg
     4261 cggcaccggt gtaaacatga tatctgcggc agcaatggca tttttcaaaa atgcgtccag
     4321 atgcgggcct gtatcaataa ggatgaagtc gaaatcatgc ttcagcttat caataatatt
     4381 ctcacgcaat atcgcgtgtt tgttttgtcc taatagatgc tcctcgcaca gagtatccca
     4441 gtttgatgca ataaaggcgt cgtcaataga agccggcata acataaacgc cagggataac
     4501 tgacggaacg ataaaatctt ccagtaattc ctcacgactc acgttctgca acatagcctg
     4561 cggtgcggtt gtatcaacca gccccaccgc atgcaagtag ttcagaaaca tagtggctga
     4621 tgactgggga tctaaatcaa ggagcagaat acgtaaatct tctgacagca ggtgaggatg
     4681 agcacgcagg gcgtgagcta cggaaacact actcacggtt ttagacacac cgccttttag
     4741 actaccgatg aatatggaat atgcctcact ataacgatcg cgatatttag gaatgccacg
     4801 atgtgcatag atatcgataa tgttctggat ggttaatgca tattttgtcg cggttcctgc
     4861 tgggcgtttt tcaaactgat agccctgagc ctccatctca ccgactgcac catccacgct
     4921 tgcgcgcgtt aattttggaa gcttagctac agcagacttt gaatagactt gataaaaaac
     4981 atcagcctga aactcctgct tttgctcctg aatctgtttt gttagggcga gcaacatttt
     5041 atctgcacgc tgcgcaacct tacttaactg ctcaaaacta gtcattcccc ctccgtgcta
     5101 tgtaggattt taacttatta caaaaaaaac tgcattagca gggtaatgca tgcaagtaca
     5161 tattgtacat gcttatatta aaaatgcgcg cattgctgat ttattaatca tcggaatgaa
     5221 tattactgta agatagtatg cctttccggg agaaagacgc tgatttcccc cgaataatcc
     5281 agttcaccac cacactccgg acaacagctt tcttcaggct ccagatggtg tatttcgcgg
     5341 gaaacatgtt ccggtaacgg acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc
     5401 ttccaatcag tagaacatgt gcttttcaat gaaggagccg atgacttttt cgtggatttc
     5461 cacggaacgc gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt
     5521 ccggttgtta cgtgcaatac gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg
     5581 cacttctctg gcgtagctgc cccagtcatc gctggtcacc atgccgatac agaacggagt
     5641 caacagggct aacagttccc gacaggtctc atcattccgt ggcccgaaag tgtaagccaa
     5701 cacaccgcca gttttagtgt tgtacgcata ccagagccag tgtt
//
LOCUS       DAZXUK010000232         5707 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZXUK010000232 DAZXUK010000000
VERSION     DAZXUK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.232"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            11..1300
                     /gene="hpaG"
                     /locus_tag="ACH3L8_003413"
     CDS             11..1300
                     /gene="hpaG"
                     /locus_tag="ACH3L8_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFT4086382.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPSDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1297..2763
                     /gene="hpaE"
                     /locus_tag="ACH3L8_003414"
     CDS             1297..2763
                     /gene="hpaE"
                     /locus_tag="ACH3L8_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFT4086383.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2765..3616
                     /gene="hpaD"
                     /locus_tag="ACH3L8_003415"
     CDS             2765..3616
                     /gene="hpaD"
                     /locus_tag="ACH3L8_003415"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFT4086384.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3626..4006
                     /locus_tag="ACH3L8_003416"
     CDS             3626..4006
                     /locus_tag="ACH3L8_003416"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFT4086385.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            4074..4877
                     /gene="hpaH"
                     /locus_tag="ACH3L8_003417"
     CDS             4074..4877
                     /gene="hpaH"
                     /locus_tag="ACH3L8_003417"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFT4086386.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4888..5691
                     /gene="hpaI"
                     /locus_tag="ACH3L8_003418"
     CDS             4888..5691
                     /gene="hpaI"
                     /locus_tag="ACH3L8_003418"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFT4086387.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
BASE COUNT         1353 a         1587 c         1640 g         1127 t
ORIGIN      
        1 aggagtggta atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca gccaacttga
       61 tgcctggcag gaagcgttcc agcaatctcc ctacaaagcc ccgcctaaaa ctgcggtctg
      121 gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct ttccacaggg
      181 tgaaaaagta ctgagcggtg cgactgttgc gctgattgtg gggaaaacgg cgacgaaagt
      241 acgtgaagaa gatgcggcag agtacatcgc cggatatgcg ctggataacg acgtcagcct
      301 gccggaagag agcttttacc gtccggcaat caaagcaaaa tgtcgtgatg gattctgccc
      361 cattggcgaa accgtggctc tcagcaatgt cgataatctg actatctata ccgagatcaa
      421 cgggcgtcct gccgatcact ggaataccgc cgatttacaa cgtaacgccg cacagctgct
      481 gagcgccctg agcgaatttg ccacgctgaa tccaagcgat gctattctgc tcggcacgcc
      541 acaggcgcgc gtggaaatac agccaagcga tcgcgtgcgt gttctcgcag aaggtttccc
      601 gccgctggaa aatccggtag tggacgaacg tgaagtgacc acgcgcaaga gcttcccaac
      661 gctgccacac ccgcacggta cgctgtttgc cctcggcctg aactacgccg accacgccag
      721 cgaactggaa tttaagccac cggaagagcc gctggtgttc ctgaaagcgc cgaataccct
      781 cactggcgac aaccagacct ccgtgcgccc gaacaatatt gaatacatgc actacgaagc
      841 ggagctggtg gtagttattg gcaagcaggc gcgtaacgtc agcgaagccg atgccatgga
      901 ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt cgcgactatc tggaaaacta
      961 ctaccgccct aacctgcggg tcaaaagccg cgacggactg acgccgatgc tttcaaccat
     1021 cgtgccgaaa gaggcgatcc cggacccgca taatctgacc cttcgcacct tcgtcaacgg
     1081 cgagttacgc cagcaaggca ccaccgccga tctgatcttc agcgtgccct tcctgatcgc
     1141 ctacttaagc gaatttatga ccctgaatcc gggcgacatg atcgccaccg gcacaccaaa
     1201 aggcttatct gacgtggtgc ctggcgatga agtagtggtg gaagtagaag gcgtgggccg
     1261 cctggtgaac cgaattgtga gtgaggaaac agcgaaatga aaaaagtaaa tcattggatc
     1321 aacggcaaaa atgttgcagg taacgactac ttccagacta ccaatccggc aacgggtgaa
     1381 gtgctggcgg atgtggcctc tggcggtgaa gcggagatca atcaggcggt agcggcagcg
     1441 aaagaggcgt tcccgaaatg ggctgatctg ccgatgaaag agcgtgcgcg cctgatgcgc
     1501 cgtctgggcg atctgatcga ccagaacgtg cctgagatcg ccgcgatgga aaccgcggac
     1561 acgggcctgc cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc tcacaacttt
     1621 gaatttttcg cggaagtctg ccagcagatg aacggcaaga cctatccggt tgacgacaag
     1681 atgctcaact acacgctggt gcaaccggta ggcgtttgtg cgctggcgtc gccgtggaac
     1741 gtgccgttta tgaccgccac ctggaaggtc gcgccgtgtc tggcgctggg caataccgcg
     1801 gtgctgaaga tgtcggaact ctccccgctg accgccgacc gcctgggtga gctggcgctg
     1861 gaagctggta ttccggcggg cgtgctaaac gtggtacagg gctacggcgc aaccgcaggc
     1921 gatgcgctgg tccgtcatca tgacgtgcgt gccgtgtcgt tcaccggcgg taccgccacc
     1981 gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggagct gggcggtaaa
     2041 tcaccggtgc tgatttttga agatgccgat attgagcgtg cgctggacgc cgccctgttc
     2101 accatcttct caatcaacgg cgaacgctgc accgccggtt cgcgcatctt tattcagcag
     2161 agcatctatc cagaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtgggc
     2221 gatccgaacg atccgaatac ccaggttagg gcgcttatca gtcagcaaca ctgggaaaaa
     2281 gtctccggct atatccgtct cggcattgaa gaaggcgcaa ccctgttggc gggcggcccg
     2341 gataaaccgt ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg cccaacggtg
     2401 ctggcggacg tagataaccg tatgcgcgtt gcccaggaag agattttcgg gccggtcgcc
     2461 tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgcc tggcaaacga cgtggagtac
     2521 ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc
     2581 attgaagcag gcatggtgtt cgtcaacacc cagaacgtgc gcgacctgcg ccagccattt
     2641 ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc
     2701 gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc
     2761 tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcga tgtatctctc
     2821 tgaactacca ggaaaaaacc acggttgtcg ccagggtgcg atcgacgggc ataaagagat
     2881 cagcaagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata ctcactggct
     2941 ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa
     3001 cgagctgccg cattttattc gcgacatgac ctacaactac gagggcaacc cggagttggg
     3061 gcagctaatt gccgaggaag ccttaaagct cggcgtgcgg gcaaaagcgc acaatattcc
     3121 cagcctgaaa ctggaatacg gcacgctggt accgatgcgc tacatgaatg aagacaagca
     3181 cttcaaagtg gtctccattt cggctttctg cacggttcac gattttgccg acagccgcaa
     3241 gctgggcgaa gcgattctga aagcgatcga acagtacgac ggcaccgtgg cggtccttgc
     3301 cagcggttcg ttatcgcacc gctttattga cgatcagcgt gcggaagaag ggatgaacag
     3361 ctacacccgc gagttcgacc gccagatgga cgagcgtgtg gtgaagctgt ggcgcgaagg
     3421 ccagttcaaa gagttttgca atatgctgcc ggagtacgcc gactactgct acggcgaagg
     3481 caatatgcac gacacggtga tgctgctggg gatgctcggc tgggataaat acgacggcaa
     3541 ggtggagttt attaccgagc tgttcccaag ctctggcacc ggtcaggtta acgctgtttt
     3601 cccgctgccc gcgtaaggac gttttatgcc gcactttatc gtggaatgta ctgaaaatat
     3661 tcgtgaagaa gcccgcttac ctgagctgtt tgccagtgtt aacactgcac tggcggcaac
     3721 ggggattttt ccgcttgggg gaatacgtag ccgggcgcac tggatcgata cctggcaaat
     3781 ggctgatggc aagcacgatt acgcctttgt gcatatgacg ctaaaaatcg gcagcggacg
     3841 cagtctggaa agccgtcagg aagtgggcga aatgctgttt gatctgatta aaacgcactt
     3901 cgcctcactg atggagagcc gttacctagc gctttcattt gagattgcag agctgcaccc
     3961 gacgttgaat ttcaaacaga acaacgtaca cgcgttgttt aaataaattt gcaccagctt
     4021 ccaggccgga tgataaggcg ctgccgcatc cggcaatgcc acaggatatc gctatgttcg
     4081 ataaacacac ccacaccctg atcgcccagc gtctggatca ggcagaaaaa cagcgcgaac
     4141 agatccgcgc gatctcgctg gattacccgg agatcaccat cgaagacgcc tacgccgtgc
     4201 agcgcgaatg ggttcgcctg aaaatcgccg aaagtcgcac gctgaaaggc cacaaaatcg
     4261 gtctgacctc gaaagcgatg caggcaagct cgcagatcag cgaaccggat tacggtgcac
     4321 tgctggacga catgttcttc cacgatggca gcgatatccc gaccgatcgc tttatcgtgc
     4381 cgcgcattga agtggagctg gcttttgtgc tggcaaaacc gctgcgtggg ccaaactgca
     4441 cgctgttcga cgtttacaac gccacggact atgtgatccc ggcgctggag ctgatcgacg
     4501 ctcgctgcca caacatcgat ccggaaaccc agcgcccgcg caaagtattc gacaccattt
     4561 ccgataacgc cgccaacgcc ggagtgatcc tcggaggtcg tccgatcaaa ccagacgaac
     4621 tggatctgcg ttggatctcc gccctgatgt atcgcaatgg cgtgattgaa gaaaccggcg
     4681 tcgccgctgg tgtgctgaat catccggcaa acggcgtggc ctggctggcg aacaaactcg
     4741 caccgtatga cgtacaactg gaaaccgggc aaatcattct cggtggttcg ttcaccctcc
     4801 cggttccggc gcgtaagggc gacaccttcc acgtcgatta cggcaacatg ggctccatta
     4861 gctgccgctt tgtttaagga gagaacgatg gaaaacagtt ttaaagcggc gctgaaagca
     4921 ggccgtccgc agattggatt atggctgggg ctgagcagca gctacagcgc ggagttactg
     4981 gccggaccag gattcgactg gttattgatc gacggtgagc acgcgccgaa taacgtgcaa
     5041 accgtgctca cccaactaca ggcgattgcg ccctacccca gccagccagt ggtacgccca
     5101 tcgtggaacg atccggtgca aatcaaacaa ctgctggacg taggcacaca aaccttactg
     5161 gtgccgatgg tacaaaacgc cgacgaagcc cgtaaagcgg tacgcgccac ccgttatccc
     5221 cccgccggta ttcgcggtgt gggcagtgcg ctggctcgcg cctcgcgctg gaatcgcatt
     5281 cctgattacc tgcaaaaagc caacgatcaa atgtgcgtgc tggtgcagat cgaaacgcgt
     5341 gaggcaatga agaacttacc gcagattctg gacgtggaag gggtcgacgg cgtgtttatc
     5401 ggcccggcgg atctgagcgc cgatatgggt tatgccggta atccgcagca cccggaagta
     5461 caggccgcca ttgagcaggc gatcgtgcag atccgcgaag cgggcaaagc gccggggatc
     5521 ctgatcgcca atgagcaact ggcaaaacgc tatctggaac tgggcgcgct gtttgtcgcc
     5581 gtcggcgttg acaccaccct gctcgcccgc gccgccgaag cgctggcagc acggtttggt
     5641 aatagttctt ctatttcgat ggcgaagcaa aacaacaact ctgtctacta aacgtacgat
     5701 gtggagc
//
LOCUS       DAZXUK010000233         5600 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZXUK010000233 DAZXUK010000000
VERSION     DAZXUK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.233"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..411
                     /locus_tag="ACH3L8_003419"
     CDS             <1..411
                     /locus_tag="ACH3L8_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFT4086388.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="ACH3L8_003420"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="ACH3L8_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACH3L8_003421"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACH3L8_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HFT4086389.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1332 g         1488 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc
//
LOCUS       DAZXUK010000234         5558 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZXUK010000234 DAZXUK010000000
VERSION     DAZXUK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.234"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..642
                     /locus_tag="ACH3L8_003422"
     CDS             <1..642
                     /locus_tag="ACH3L8_003422"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HFT4086390.1"
                     /translation="VVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAIT
                     TGKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQN
                     AVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCA
                     LGLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(696..1301)
                     /gene="azoR"
                     /locus_tag="ACH3L8_003423"
     CDS             complement(696..1301)
                     /gene="azoR"
                     /locus_tag="ACH3L8_003423"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFT4086391.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1502..5404
                     /gene="hrpA"
                     /locus_tag="ACH3L8_003424"
     CDS             1502..5404
                     /gene="hrpA"
                     /locus_tag="ACH3L8_003424"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFT4086392.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1376 a         1360 c         1540 g         1282 t
ORIGIN      
        1 gtcgtaggcg cgtgcattgt gttgatggag ctaatgatga tggttcagtt atggtggtca
       61 gtctggttat gttggccagt attatcgcta ctcattattg gccgtgggtg cggtgggctt
      121 ggcgcgataa caacagggaa agatagtcag gggaaactcc cgcccgccgt ttactggctg
      181 acattgccct ggcgcatcgg gatgtggcta tctatgcgtt ggttttgtcg tcgcctggag
      241 ccggtgagca aaatgactgc tggtgtttat ttaagggcgt ttccacgaca tattccggca
      301 cagaatgcgg ttctggacgt cacctttgaa ttccctcgtg gacgagcgac aaaagatcga
      361 ctctatttct gtgtaccgat gttggatctg gtggttccgg aagaggggga gctccgacag
      421 gccgtggcga tgctggaaac attacgcgaa gagcaaggca gcgttctggt ccattgcgca
      481 ttgggattat cgcgcagtgc gctggtagtg gcggcatggc tgttatgtta cggacactgt
      541 aaaacagttg atgaagcgat tatctttatt cgagccagac gctcgcatat tgtgcttaag
      601 gaagagcaca aagcgatgtt gaaattatgg gaaaacaggt aagtggattg agatgtggac
      661 tgaatatcta cagtccacat caagaccgtg tccggttatg cagcaacaat gctgtcgatg
      721 gctgcttttg cgtcagactg tgctttcgct gccatttccg gaccgtatgc gatcccttcg
      781 gcgaagacaa atttcacatc ggtaatgccg ataaagccga ggaacgtgga cagatacggc
      841 gtcaccaggt ccgttggtcc atctttgtgg atcccaccgc ggctggtaat aacgatggct
      901 tttttacccg ttaccagacc ttccggaccg ttctcggtat agcggaaagt aacgcctgcg
      961 cgtgcaacca ggtcaaaata gtttttcaac tgagttgaga tgttgaagtt atacatcggt
     1021 gccgcaataa cgataacgtc gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc
     1081 agagcttcct gctgacgcgg agtcagcggc gcatcgctcg gacgcagagc gccaaccagt
     1141 tcgccatcca gtaccggaat cggatttgca gctaggtcgc gaacggtgat ttcatcagcg
     1201 gagtgctttt cgctccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac
     1261 cctgccagga tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatgt
     1321 ttgaatggac gatgccccgt tgcttgctga cactttattc acaatcctgc cacagagatc
     1381 gcgcaataaa tcgaagtcta tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt
     1441 ggtactctat atctatcatt taaaagaaaa ttaatcaggc agactactgc ccactaacgt
     1501 tatgacagaa caacaaaaat tgacctttac tgccttgcaa cagcggctgg attcgctgat
     1561 gctgcgcgac agactgcgtt tttctcgccg tctgcattgt gttaagaagg ttaaaaatcc
     1621 tgatgcacaa caggccattt tccaggagat ggcgaaagag attgaccagg cggcagggaa
     1681 agtcctgctg cgtgaagcgg cacgaccgga aatcacttat cctgacaatt taccggttag
     1741 tcagaaaaaa caggacatac tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg
     1801 ggaaaccggc tccggtaaaa cgactcagct accgaaaatc tgtatggagc tggggcgcgg
     1861 tattaaaggg ctgatcggtc atacccagcc gcgtcgcctg gcggcacgaa cggtggccaa
     1921 ccgtattgcg gaagagctga aaacggagcc gggcggttgc atcggttaca aagtgcgttt
     1981 cagcgatcac gtaagtgata acacgatggt caagctgatg accgacggga tcctgctggc
     2041 ggagatccag caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcgca
     2101 cgaacgcagc ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg
     2161 tcctgaccta aaaatcatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca
     2221 ctttaataat gcgccgatta ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta
     2281 tcgcccgatt gttgaagaag ccgatgacac cgagcgcgat cagttgcagg cgatttttga
     2341 cgccgtagac gaactaagcc aggaaagccc cggcgacatt ctgatcttta tgagcggcga
     2401 gcgggaaatt cgcgataccg ccgatgcgct gaacaagctg aacttacgcc ataccgaaat
     2461 cttgccgctt tatgcgcggc tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag
     2521 cggacggcgc attgtgctgg cgaccaacgt cgcggaaacc tcgctgaccg taccggggat
     2581 taaatacgtc atcgaccccg gtacagcgcg tatcagccgc tacagctatc gcaccaaagt
     2641 gcagcgtttg ccgattgaac ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg
     2701 tggtcgtgtg tccgaaggga tctgtattcg tctctattcc gaagacgatt tcctctcgcg
     2761 cccggagttt accgatccgg agattctgcg taccaacctg gcctcggtta ttttgcagat
     2821 gaccgcgctg gggctgggcg atatcgctgc gttcccgttt gtcgaagcac cggataaacg
     2881 caatatccag gatggcgtgc gtctgctcga agaactgggc gcgatcacca cggatgaaca
     2941 ggccagcgcc tataaactga cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc
     3001 acgtctggcg cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat
     3061 tatcacgtcc gcgctctcca tccaggatcc gcgcgaacgt ccgatggaca aacagcaggc
     3121 atcggacgaa aaacatcgtc gcttccacga caaagaatcc gacttcctcg cgtttgtaaa
     3181 tctgtggaat tatcttggcg agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct
     3241 gtgtcgtacc gattacctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca
     3301 gttgcgccag gtggtgaaag aacttggcat tccggtcaac agcgaaccgg cggagtatcg
     3361 tgaaattcac atcgccttac tgaccggttt gctttcccat atcggcatga aagatgccga
     3421 taaacaggaa tataccggtg cacgtaacgc gcgtttctcc atcttccccg gttctggttt
     3481 attcaaaaag ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg
     3541 ggggcgcatt gctgcgcgta tcgacccgga atgggtagaa cccgttgctc agcatttgat
     3601 taaacgcacc tacagcgaac cgcactggga acgggcgcag ggcgcggtga tggcaacgga
     3661 aaaagtcact gtttatggtt tgccgattgt tgctgcgcgc aaggtcaact acagccagat
     3721 cgattcggcg ttatgtcgtg aactctttat tcgccacgct ctggtggaag gtgactggca
     3781 gacgcgtcac gcattcttcc gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga
     3841 acacaaatca cgtcgccgcg atattctggt tgatgacgaa acgctgtttg agttctacga
     3901 ccagcgcatc agccacgatg taatctccgc tcgtcacttc gatagctggt ggaaaaaagt
     3961 cagccgcgaa acgcctgatt tgctcaactt tgaaaagagc atgttgatca aagaaggggc
     4021 ggaaaaaatc agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg
     4081 tttgagctat cagtttgagc caggcgcgga cgcggacggt gtgaccgtac atattccgct
     4141 gccgttactt aaccaggttg aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg
     4201 cgaattggtg attgctctga ttaaatcgtt gccgaaaccg gtacgccgta attttgtgcc
     4261 tgcaccaaac tacgccgaag cgtttttagg ccgcgtcaca ccgctggagt taccgttgct
     4321 cgacagcctt gagcgcgagt tacggcggat gaccggcgtt accgttgacc gcgaagactg
     4381 gcactgggat caggtgcccg atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa
     4441 caagaagcta aaagaagggc gctcgctaca agatctgaaa gatgcgctga aaggcaaagt
     4501 gcaggaaacg ctgtccgcgg tggcggatga cggtatcgag cagagcggct tacatatctg
     4561 gagttttggt cagctgccgg aaagttacga acagaagcgt ggcaactata aagtgaaggc
     4621 gtggccggcg ctggtggatg aacgcgacag cgtgacgatc aaactgtttg ataacccgct
     4681 ggagcaaaaa caggcaatgt ggaatggtct tcgccgtcta ctgctgctaa atatttcctc
     4741 gccgatcaag tatttacatg agaagttacc aaacaaagcc aagctggggc tgtactttaa
     4801 cccgtatggc aaagtgctgg agctgatcga cgactgtatc tcctgcggtg tggatcaact
     4861 gatcgacgcc aatggtggcc cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa
     4921 agtgcgcgcc gaactgaatg acacggtggt ggatattgcg aagcaggtcg agcaaatcct
     4981 cactgcggtg ttcaatatca acaaacgtct gaaagggcgg gtggatatga ccatggcact
     5041 ggggctttct gatattaaag cgcagatggg cgggttggta tatcgcggtt ttgtcactgg
     5101 taacggcttc aaacggctgg gcgacacgct gcgatatttg caggcgattg aaaaacgact
     5161 ggaaaaactg gcggttgatc cacatcgcga tcgtgcacag atgctgaaag tcgaaaacgt
     5221 ccagcaggcg tggcagcaat ggatcaacaa actgccgcct gcacgtcgtg aggatgaaga
     5281 cgtgaaagag atccgttgga tgatagaaga gctgcgcgtt agttacttcg ctcaacaact
     5341 tggtacgcct tatccgattt cagataagcg tattttgcag gcgatggagc agattagcgg
     5401 ttaaccctgc tatttgccta ataaatctaa aacccggtaa gcatttagcg ccgggttttt
     5461 tattaattct aaaacggtaa ggacaaaaat tcaggaattc agaaaaatac aattctcagc
     5521 tgcaagatga ataatgttta tctacagcat ttccttaa
//
LOCUS       DAZXUK010000235         5537 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZXUK010000235 DAZXUK010000000
VERSION     DAZXUK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.235"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(167..1126)
                     /gene="nrdF"
                     /locus_tag="ACH3L8_003425"
     CDS             complement(167..1126)
                     /gene="nrdF"
                     /locus_tag="ACH3L8_003425"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFT4086393.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1136..3280)
                     /gene="nrdE"
                     /locus_tag="ACH3L8_003426"
     CDS             complement(1136..3280)
                     /gene="nrdE"
                     /locus_tag="ACH3L8_003426"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFT4086394.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3253..3663)
                     /gene="nrdI"
                     /locus_tag="ACH3L8_003427"
     CDS             complement(3253..3663)
                     /gene="nrdI"
                     /locus_tag="ACH3L8_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFT4086395.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3660..3905)
                     /gene="nrdH"
                     /locus_tag="ACH3L8_003428"
     CDS             complement(3660..3905)
                     /gene="nrdH"
                     /locus_tag="ACH3L8_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFT4086396.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4153..4482)
                     /locus_tag="ACH3L8_003429"
                     /pseudo
     CDS             complement(4153..4482)
                     /locus_tag="ACH3L8_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
     gene            4634..4978
                     /locus_tag="ACH3L8_003430"
     CDS             4634..4978
                     /locus_tag="ACH3L8_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFT4086397.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5015..5464)
                     /gene="alaE"
                     /locus_tag="ACH3L8_003431"
     CDS             complement(5015..5464)
                     /gene="alaE"
                     /locus_tag="ACH3L8_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFT4086398.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1404 a         1383 c         1383 g         1367 t
ORIGIN      
        1 tattttttgt catagatagc gaaaaaacgc ctgaatattg tagtttcagg cgaataatgc
       61 aacagagtat cagtgtagat cactacagaa tatttgcgtg ataaaaagct atttggcggg
      121 aattatttcc ctgctgcggg tagtgatatt tttgaaaata acaccctcag aaattccagt
      181 cttcatcttc tgtttcaacc gctttcccca tcacataaga ggagcctgaa ccggaaaaga
      241 aatcgtgatt ttcatcggca ttcggcgaaa gcgcggcgag gattgccgga ttcacttccg
      301 ccatttctgc gggaaataac ggttcgtagc ccagattcat caaagcctta ttggtgttgt
      361 aacagagaaa cgctttcaca tcgtcagccc acggggtttc ggcgtacagc tcatcggtgt
      421 attgcaactc gttgtcgtag agttccagca acaaatcgaa ggcgaaactc ttcaactctt
      481 cacgttgtcc cagagatatc ttttccatgt ttttctgata tttatagcct atgtagtaac
      541 cgtggactgc ttcatcgcgg ataatcagac ggatcaggtc cgcggtattg gtcagctttc
      601 cgcggctgga aaaatacatc ggcagccaga aaccggaata gaacaaaaaa gattcaagaa
      661 acacactggc gattttcttt ttcagcggat catcaccgcg ataatgttgc tgaataatct
      721 gagcttttcg ctgcaacggt gcgttttctt cactccaggc gtaggcggca tcgacatctt
      781 tggtctggca cagcgtcgag aaaatcgaac tgtaagagcg ggcatgaacc gcttccataa
      841 agctgatatt cgataatacc gcctcttcat gaggcgtgag cgcatcgggc atcagcgaag
      901 gcgcgccgat aacattttgc agcgtgtcga gcagcgtcag gccagtaaaa acgcgcatcg
      961 tcagttgttg ttctacgaca gttaatgtct gccaggcggg aatatcgttc gacagcggca
     1021 ccttttctgg tagccagaaa ttgctggtca ggcgattcca cacctccaga tctttatcgt
     1081 cagatatctt gttccagttg atggcgctga tacgtgagag tttcatagat attccttaaa
     1141 gtgcacagga gacgcagcct tcaatttcag tgccttccag cgccatctga cgcaggcgga
     1201 tgtaatagag cgttttgata cctttgcgcc aggcgtaaat ctgcgctttg ttgatatcgc
     1261 gagtggtggc ggtatcgggg aaaaacagcg tcagcgacag cccctgatcg acatggcgag
     1321 tcgcttccgc gtaggtgtcg atgatctttt ctgcgccaat ttcgtaagcg tcctgataca
     1381 gcgccagatt ttcgttagtc ataaacgggg cagggtagta aacgcgtcct gttttgccct
     1441 ctttgcgtat ctctactttc gccacaatcg gatgaatact cgacgtagca tggttgatat
     1501 aagagataga cccggttggc ggcaccgcct ggagattctg gttgtatatg ccgtagcgca
     1561 tcacgtcgtc gcgcagttgc gcccacatct cacgggtagg taacgtaata cagctgcggg
     1621 caaacagttc gccaactttc gccgttttcg gctgccagtt accttgcaga tattggctga
     1681 aatattcacc actggcatag cgtgactgtt tgaacccggc gaaggtttca ccgcgttcgc
     1741 gcgccagcaa catcgaggta cgcagtgcgt gccaggtgat ggtatagaaa tagagattgg
     1801 tgaaatccag ggcttccggc gaaccataag cgatgccttc cctcgccaga taaccatgta
     1861 aattcatctg ccccagtccg atggcgtgcg aggcggcatt tccggcttcg atggacggca
     1921 cgctgcggat atgactcata tctgacaccg ccgttaaacc gcgcacggca gtctctaccg
     1981 tgcgggaaaa atcgggggaa tccatggtgt gcgcaatatt caacgaacct aaattgcagg
     2041 aaatatcatg gcctgtgcgg gcatagtcga gattctcgtc atactctgag gcgctgttaa
     2101 cctgcaaaat ttctgagcag agattactca tatttatgcg cccggcgata gggttagcac
     2161 ggtttactgt gtcttcatac atgatgtagg gatagccgga ctcaaactgg atttctgcca
     2221 gtcgctggaa gaaatcacgg gcgttgaggt attttttgcg gatgcgttca tcggcaacca
     2281 gttcgtcata gtgttcgcta atggcgatat cggcaaacgg cttgccataa actcgctcaa
     2341 cgtcataggg cgaaaacagc gccatctgcg cattctcttt tgccagatgg aaagtgatat
     2401 ccgggatcac cacgcccagc gacagtgttt taatgcggat tttttcgtcg gcattttccc
     2461 gtttcgtgtc gagaaaacgc agaatatcgg gatgatgagc atgtaaatag actgcacctg
     2521 ccccctgacg cgcgccgagt tggttggcat aggaaaatgc gtcttccaac attttcatca
     2581 ccggaattac gccagaagat tgattttcaa tacgtttaat tggcgcgccc gcttcccgca
     2641 gattcgacag caaaaatgct acgccgccgc cgcgtttcga cagctgcagt gcggaattta
     2701 ccgcccgacc aatcgactcc atattgtctt caatacgcag caaaaaacag gaaaccagtt
     2761 cgccgcgctg ctgcttaccg cagttgagga atgttggcgt ggctggctga aagcgtcctg
     2821 acagcatttc atcggtcagt tgcaacgcca gcgtctcatc gccttgtgcc agcgtcagcg
     2881 ccaccatcgt gactcgatcg gcaaaatctt ccagataacg tttaccgtcg aatgtcttca
     2941 acgtatagct ggtgtaaaac ttccatgccc cgaggaatgt ctggaaacga aaaccgctgg
     3001 tgtgtgcgtg ggcaaacagc gtaatgacaa agtcgcgaga gtagcgatta agaacgcttt
     3061 catcatagta accttcgttg accagccagt tcaggcgctg ctgctggcta ctgaaggtca
     3121 cactgttcgg acgcacatgc gtcgcaataa aggcgtcaac ggcctggcgg tctttatcga
     3181 actgaatgcg acctgcgcta tcgtaaagat taagcatcgc attcagcgcg tggtaatcca
     3241 tcgtttcctg cgtcaggcat tctgcggttg tcgttgccaa aattcggtta ctcctttacg
     3301 aacgttttcg atatcgcttt gcgtacccat gagttcaaaa cggtacagcc acggcacgcc
     3361 gcatttccgg gcaatcacat ctccggcgcg gccatacgcc tcaccaaagt tgcgattacc
     3421 agaagcaata acgccgcgaa gcaacgcccg gttgtgctcg tcgtttaaaa agcgaattac
     3481 ctgtcgtggc accgcgccag ccgtaccgcc gccgccgtaa gagggcacga tcaggatgta
     3541 aggctcgtct acctgaatcc gttcccgctc attgagcggg atgcgcaccg cgggcagacc
     3601 taaacgttcg ataaaacgct gcgtgttttc ggagctgctg gagaagtaga cgagctggct
     3661 catgcactgg ccgcgtgtgg cgctggatgc agacggttaa tcatgtccgg acggaaacca
     3721 gaccagctaa gatcgccagc aatcactacc ggcaactgac gaaagccctg agcacgcaac
     3781 gcttccgctg cttcaggaac gcgatcgaca ttaatcattt caaaatcaaa gccccggttt
     3841 tccatcgccc gtttggtggc gtggcactga acgcaatcgt tacgagtata aatagttatg
     3901 cgcatgattc gtatttccgt ttaaaatgaa gatacggcgc gatgatacgc gtcgggttgt
     3961 ctctctgttg atacagagat actagatgta gttgaaaaaa gattcaacta cacaatatat
     4021 agcaaatagt gatcgaaatt accctggata tgagcgtgac gggggagggg gatttttgtg
     4081 attcaccagg caaaaagaaa ccccgaagac aggcttcggg gtcaaagacg cgtatttatt
     4141 atcatttttg cactacgatt tgcgcatgct taacagtgcg ccgataaaaa tacctaccgc
     4201 agctgctgta cccacgctac accagggtct ttcacgaaca aaagaatccg ctcagccaac
     4261 ggcatcgcgc gcggcttgct ggacgcgagt acgaccatgc attcgcgccc tggtttcttt
     4321 cagcaacgcc tgagctttac tgcgtgcggc ttcagcttcc cctttggcgt cgctgcccca
     4381 ggacttcaat acagattcga ggctgtcagc taattgattg acatcattct ggatatcctg
     4441 cacgccatca tcaacgtcgt tgcggttcgg tcggttaaac atatgatccc ccattagaat
     4501 tcgatgtaat tttcagtgta gaccataatt ttcttaactg aggttcatcg cggctttatg
     4561 gacttttctg aaagcgttgt gaatgcgcaa tcaggtaagg taggcccgcg gttgaaaata
     4621 acgaggatca aatatgtatt tacgaccaga cgaggtggcg cgcgtacttg aaaaagtcgg
     4681 ttttactgtc gatgtggtaa cccagaaagc gtatggttac cgccgtgggg aaaattatgt
     4741 ctatgttaat cgcgaagcgc gaatgggacg taccgctctg gtaattcatc caacattaaa
     4801 agaacgcagt tcaacactgg ctgaacccgc ttccgatatt aaaacttgcg atcattacca
     4861 gcaatttccg ctctatttag caggcgagcg acacgagcat tacggtatcc cgcatggctt
     4921 tagttcgcgt gttgcgcttg aacgttattt gaatggttta tttggcgaag ccagttaaag
     4981 acgcgactgg cgatgccagt cgcgaaaaga agagtcaggc ttttacctgc tggtaacggc
     5041 tgactttaaa cagtcggcgg caataatcga ggaagtagcc ataaaccgcc cccatcaaca
     5101 tcgaaacaac gatgtttgaa ctgaccgccg ccataatctg atgccagtct gcgcccacca
     5161 ctaacaagat cgccacatac accggtgact ggaacgtcac ataagccagg atatcagcca
     5221 gattttttat ccagcccgac gggctaactt tgcgtgccgc gcgcataaac agatcgcggt
     5281 acataccgta tggccatgca attaagatgt tcaccggaat cgctaccaat ctggaataaa
     5341 aagactgttc gaagctcatt ccggagagga aaacttcaat acacatgttc acgacagaac
     5401 agtaaacaac catcgcgaac gtatctgcaa ctgcatgacg caagcgtgac tgcggtgaga
     5461 acatgctaat gctccttaat aaaagcgtaa tgggatgtta atggagatga aagcagtgga
     5521 tctcattgcc atctgca
//
LOCUS       DAZXUK010000236         5515 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZXUK010000236 DAZXUK010000000
VERSION     DAZXUK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.236"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..70
                     /gene="ldrD"
                     /locus_tag="ACH3L8_003432"
     CDS             <1..70
                     /gene="ldrD"
                     /locus_tag="ACH3L8_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HFT4086399.1"
                     /translation="LAAPVIAGILASMIVNWLNKRK"
     gene            446..553
                     /gene="ldrD"
                     /locus_tag="ACH3L8_003433"
     CDS             446..553
                     /gene="ldrD"
                     /locus_tag="ACH3L8_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HFT4086400.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            929..1036
                     /locus_tag="ACH3L8_003434"
     CDS             929..1036
                     /locus_tag="ACH3L8_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFT4086401.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            1412..1519
                     /locus_tag="ACH3L8_003435"
     CDS             1412..1519
                     /locus_tag="ACH3L8_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFT4086402.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(1606..3285)
                     /gene="bcsG"
                     /locus_tag="ACH3L8_003436"
     CDS             complement(1606..3285)
                     /gene="bcsG"
                     /locus_tag="ACH3L8_003436"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFT4086403.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(3282..3473)
                     /gene="bcsF"
                     /locus_tag="ACH3L8_003437"
     CDS             complement(3282..3473)
                     /gene="bcsF"
                     /locus_tag="ACH3L8_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFT4086404.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(3470..5041)
                     /gene="bcsE"
                     /locus_tag="ACH3L8_003438"
     CDS             complement(3470..5041)
                     /gene="bcsE"
                     /locus_tag="ACH3L8_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFT4086405.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            5314..5502
                     /gene="bcsR"
                     /locus_tag="ACH3L8_003439"
     CDS             5314..5502
                     /gene="bcsR"
                     /locus_tag="ACH3L8_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFT4086406.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         1433 a         1336 c         1457 g         1289 t
ORIGIN      
        1 tttagcggct ccggtcattg ctggcattct tgccagtatg atcgtgaact ggctgaacaa
       61 gcggaagtag cgtgtcatgt gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca
      121 ggccgcaggg gtgaaactct gcggcctttt tcgtaagaaa aatgcaacgg ttgcaagcgg
      181 ataatattct gcggaatgcg cagcacagag tgaagatgag acggattccg gaacagaaaa
      241 acagtcgtag aatcagcgtc aagccggaag gattccggtg aggcgcaatg ttgcgggggc
      301 ttaatccctg gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca
      361 cctgacaaca ccacgggggc cattcttgca tccaaacaat acaagaatag ccgcgaaacg
      421 ttgtcattac aatacaggcg gctatatgac gttcgcagaa ctgggcatgg ccttctggca
      481 tgatttagcg gctccggtca ttgctggtat tcttgccagt atgatcgtga actggctgaa
      541 caagcggaag taacgtgcca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga
      601 ccaggccgca ggggtgaaac tctgcggcct ttttcgtaag aaaaatgcaa cggttgcaag
      661 cggataatat tctgcggaat gcgcagcaca gagtgaagac gtgacggatt ccggaacaga
      721 aaaacagtcg tagaatcagc gtcaagccgg aagggttccg gtgaggcgca atgttgcggg
      781 ggctttatcc ctggtggcat tggttgctgg agagagaaaa cccccgcacg ttgcaggtat
      841 gcacctgaca acatcacggg ggccattctt gaacctgaac aactcaagaa tagccgcgaa
      901 acgttgtcat tacaacacag gcggctatat gacgctcgca gaactgggca tggccttctg
      961 gcatgattta gcggctccgg tcattgctgg cattcttgcc agtatgatcg tgaactggct
     1021 gaacaagcgg aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca atttgcgccc
     1081 ggaccaggcc gcaggggtga aactctgcgg cctttttcgt aagaaaaatg caacgattgc
     1141 acgcggataa tattctgcgg aatgcgcagc acagagtgaa gacgtgacgg attccggaac
     1201 agaaaaacag tcgtagaatc agcggcaagc cggaaaggtt ccggtgaggc gcaatgttgc
     1261 gggggcttta tccctggtgg cattggttgc tggagagaga aaacccccgc acgttgcagg
     1321 tatgcacctg acaacatcac gggggctaat cttgaaccca aacaactcaa gaatagccgc
     1381 gaaacgttgg cattacaaca caggcggcta tatgacgctc gcagaactgg gcatggcctt
     1441 ctggcatgat ttagcggctc cggtcattgc tggcattctt gccagtatga tcgtgaactg
     1501 gctgaataag cggaagtaac gtgtcatgcg ggcgtcaggc tgccgtaatg gcaatttgcg
     1561 cccggatcag gccgcagagg ttaaactctg cggccttttt cgtgcttact gcgggtaagg
     1621 cacccagtcg ccgccgttca ggcgaacgta cggtttatct tggtattgaa taactactgc
     1681 atttgagttc tcggagaccg gtgctgtttg tggcaaccca ctggtgagtt ttttccagtc
     1741 aacattgtct tcggtgaaaa tcttgccatc aagaacgcga accaccagat cggagatagc
     1801 caggaagctg ctcggttggt caatgacaat cggtgccccc tgatgtggtg ccttcatgcc
     1861 gaagaatttc accccaacgg ggacgtctgt gatagacggg ctagggatat cacgtaggcc
     1921 agatacctgc attctgtcgc ccttcagcgc gccgccgtgt tccggcacca cgaccaccat
     1981 cactttacga cccgatttct ccagttcagt aaagaaggcg tccagttcat caaagaattt
     2041 ctgcgcccgc gctttgtaat ccgctgtttt gctgaccccc ggataatggt tgccgtcatg
     2101 cagtggaagc gtgttgtaga acgtggcact acggctgttt ttatcttttt cggtaacgtc
     2161 cagccagcgg ttaagcacgg cggtatcgtc ataaaccggc gaaccatcaa agcccagcaa
     2221 aataaccggc agatttgttt gatccatcaa ttcagtctgc atgccgccat tttcgcgaac
     2281 ttctttcaaa aaaccgccga actggccgtt atgtcccatc atcaggtgct gggtaaagcc
     2341 cagtttcgaa aggttatcaa acagatagca gtcgttattt gccggttgat acagattagt
     2401 gtgcgaagtc tgcccgcagc tggcgcgcag taaacggatc gccgccgggc cactgtagga
     2461 ggtggcggag ttaaagttct tgaaatcaat atcgaaatgc gaccacagtg gatgcgacat
     2521 caacccggcg gcttctatat ccgaccagga aagcgaacag atgttaatca ccagtagttc
     2581 aaatggctga gcatcagcgg gcagcgaaga cgggaaggtc gatttacgtt tcgcctccgc
     2641 gttatagaaa ttattcagcc aggcgttaag gttcgccgtt gttggcggtg cagtttgtgc
     2701 gggcatatca cccactaccg gtgcgccacc cgtcgccgca acggttgccg ctgcgttacc
     2761 acccgtcgtt gttacagtgg tcgtcggttg tccggctggc cacaaggaga aacttggtcc
     2821 cgccagggta agtacgttca gccatagcag tatggcaacc acaaaaacgg taatgcgaat
     2881 ccattgtgac aggaataacc aggccactaa taaaacaaaa atggccccaa tcatctgcca
     2941 gttaataaag cgtgtgacaa ggtcgattaa ataatcggta ctgaaccccg ccacctgcga
     3001 accctggctc attatgcttt ccgggccagg caaccagatg tcatgccaga acaaagcaaa
     3061 gccgatcggc agggcaatcc agtggcgcaa gcgatgcagg ctgtaacgcg gaatgggcat
     3121 cagcagaaac gcggcaaaca ccaaattgag gagcgggtgg aagttaagat atcccgccca
     3181 caacaggcca aacttaacca gaaaatagaa gttccagccg gaaaggccgc gccagtattg
     3241 ccagagggaa gaaggcatgg cggtattttg cgtaaattga gtcatttttt ggttgccctg
     3301 gctttttccg tgcggcgtaa cgtcccggcc ggtttaacat aacgaggttt agcaaagaac
     3361 aaacgtaagg tgtcgcgaat gcgtcgcaaa gagtgccgcg ccagatagcc cagcgggaaa
     3421 aatatcagtg cgcaaacgat aataatttca atgatatcgc tgatggtcat catgatgagc
     3481 gctccacagc atcatctaac agtcgcatgg gttctggtat tcgtcgccag tggcgaccat
     3541 cgtgctcggc attgatgacc ggtttagaac tttgcgttaa aggcagcggc attccccatt
     3601 gttctggggc aagcaggcgc atctgcacca gctcggcact gatttgatca tcttcaaacc
     3661 agaccatacg gtttgagaaa atgtcgccag taggcaatgg gaaaatatga ttcaacgcgg
     3721 tatccagatc gttaatccga cagaatgaga gaaacagcac cagccgatta ccgccaatgg
     3781 tcatgatatc gccggtacgg ttagggcgac atagcgtcag ggcttgctca acgcggatac
     3841 ccggtaccgg acgtagggca accagaacgc ctttaccgtg ggcaggcaag agagggttat
     3901 tcatcatgtt gttgacggca ttacagaaca catcccactt ctggaaacca cgcagtttga
     3961 gcggctgggt cattgacagc aaggtagtga tatcttccgg tacatagcga ctaaacttct
     4021 gcccttgcac gctttcgatc atcgtcagac aacgggagag tggcgcattc cacggaataa
     4081 ccatatttgc accgcaggcc aataacaaac gttcatcggt ggcgcgcagg ctagcggtat
     4141 tttcccgcac gaggattttc atcgcactac cgcgctggcg acgcagggta tgaatgctgc
     4201 gggccagtgg ctcgatttgc gcattttgct gtaaagaaaa gaccaccgtc gccgcctgag
     4261 cggtacgggc ttcattgaac aggacttcgt tattgttgaa cagttgccag tgttccgata
     4321 gcggcggcgc accttccagt acagcaacat tactcagaat gcgtttttcg tcgctgcgtg
     4381 gttggatctc cgcctcttcg ctttgaacta atgtccagat accattttgt tgctgaacgc
     4441 taagctgctg acgggcgctg accccttttt cgttgcacca gaaggcaata tccagcaaat
     4501 gttggtcacc ctgaaaacgc aaactggcaa gaccaaaaag tgaacggtat tcctcaagca
     4561 acaatgaaaa ttgtttatcg ttattatttc cgggattaat taccaaaagc gaacaatggt
     4621 ttaacctgct ccatttattc attttatcca accatgagcg aagccgctcg gcaggaatat
     4681 tttgccatgc gttatttgcg caaacaagaa taaaaaggta attatgggga tcaatagaac
     4741 actgcaaatc acgggccaaa tagtatagac ctttttcatg atttagcatt gaaaataatt
     4801 ttattttttc cggcccttga gaatcatcta attgaaagat tttcgccgga tcgctgtcca
     4861 tgctaatgac cgcgacgtgt gcggtttcag cctgggatgc aattgtttga ttcgccagac
     4921 tgatagcatc ttcatggcga tcgacgttaa accaccagac gccgcctgct ggcatatgtc
     4981 gcagctcatc ccataatgat gagataccga tagagaatac agggtccaca atgtccctca
     5041 tgtcaccatt ttgtctgatt atcagtttac tagcgaaagc taagaaataa acctaccatt
     5101 gaaattaaaa cttatcagat ttagcatgtg aatcaattta gaaggtcaca agacgtgtga
     5161 ttttagtctt ttcgttttta actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa
     5221 attcccgtct cgccgtaaac aataaaccgg cgaaatgctc acggttagaa ttgtctcatg
     5281 aatggtacgg ttatttcata gggatcaagc aaaatgaata acaatgaacc agatactctg
     5341 cctgatcccg cgataggcta tatcttccag aatgatattg tggcgttaaa gcaggcattt
     5401 tcactgcctg atattgatta tgccgatatt tcccaacgcg aacagttggc cgcggcatta
     5461 aaacgctggc cgttgctggc agagtttgcg caacaaaagt aggggattgg tgaat
//
LOCUS       DAZXUK010000237         5492 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZXUK010000237 DAZXUK010000000
VERSION     DAZXUK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.237"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(15..896)
                     /gene="prmA"
                     /locus_tag="ACH3L8_003440"
     CDS             complement(15..896)
                     /gene="prmA"
                     /locus_tag="ACH3L8_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFT4086407.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(908..2359)
                     /gene="panF"
                     /locus_tag="ACH3L8_003441"
     CDS             complement(908..2359)
                     /gene="panF"
                     /locus_tag="ACH3L8_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFT4086408.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2349..2591)
                     /locus_tag="ACH3L8_003442"
     CDS             complement(2349..2591)
                     /locus_tag="ACH3L8_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFT4086409.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2700..4049)
                     /gene="accC"
                     /locus_tag="ACH3L8_003443"
     CDS             complement(2700..4049)
                     /gene="accC"
                     /locus_tag="ACH3L8_003443"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFT4086410.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4060..4530)
                     /gene="accB"
                     /locus_tag="ACH3L8_003444"
     CDS             complement(4060..4530)
                     /gene="accB"
                     /locus_tag="ACH3L8_003444"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFT4086411.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
BASE COUNT         1369 a         1461 c         1402 g         1260 t
ORIGIN      
        1 cggcgatgcg aaggtcaatt cttacgaccg gtaatacggc accactcttc tttttccacg
       61 accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc ctggcttgcc
      121 agaataccgg aaaggcccag caaaccgcct gaaactggca ggacgctgat taacggtgcc
      181 agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt catttcttct
      241 ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg ttcggcgtta
      301 tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt tgctgcaccc
      361 agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat gactgtttta
      421 ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg atgggtaccc
      481 gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc cggcacatca
      541 cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa attatccatc
      601 cattcgcgct cccagtcttt atcttctagt tgttcgattt tatgcgcgaa gcctgcgccg
      661 agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc agcgtcgaac
      721 agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg ctcaaatact
      781 ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat cagcgcatcg
      841 ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca aggcatggca
      901 aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta ccgaaacggt
      961 ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg tggaagccca
     1021 ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg atcatcgcac
     1081 ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc ggccacagga
     1141 aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt tctggcggct
     1201 tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc gacatccgct
     1261 tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga tctttaatga
     1321 tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca gccataggtg
     1381 cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac gtcgggatca
     1441 ccagatctgg tacggtgaga tcggggatca ctgcacgacc taacgctccg gccaggtgca
     1501 taccgaacat caggattgcg actataatcg taccgatgat gatcccccgg tgtactgctt
     1561 tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc acgccaaagc
     1621 acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca gcgccttgtg
     1681 gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt aagccgccag
     1741 cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca agcccttgca
     1801 tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac gcaatgctga
     1861 taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc agcaggcgcg
     1921 caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa ctcaaactcg
     1981 ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc agcgtcactg
     2041 cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt gaaagccaga
     2101 cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta taagcagctc
     2161 ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagagtc atcgccagca
     2221 caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg ccggtgctcc
     2281 gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg accagcggta
     2341 gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat aaatttcacc
     2401 atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat ctcaaaccag
     2461 cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcgtctac taaccaaact
     2521 gccagataca aaagggtcag ccccagcgcc cagcgggcct ctttatgggc ctgaacaaaa
     2581 cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc ttatgggggt
     2641 tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct tttgcagtct
     2701 tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc accatgctgg
     2761 aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat accgtcgatg
     2821 atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt ttcaccgtag
     2881 caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc cgcgtagata
     2941 tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat tttgcctggg
     3001 cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc atggccgcga
     3061 acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg cagctgttct
     3121 ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg aatacgggtg
     3181 ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt acctgcaccg
     3241 cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg acgcagttcc
     3301 ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg ctgcatggag
     3361 cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac ctgaatctcg
     3421 acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct gaaagcagct
     3481 ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc gcgcactacg
     3541 cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata accaatgcgt
     3601 ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga accaggtacg
     3661 caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc catcaggcga
     3721 atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg ctcggcaaag
     3781 ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc ggtgatttca
     3841 gcggcgctga tgattgccgg gatgttcaga taacttttta ctgacggagc agggccaata
     3901 cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgtgct ggagtgcaca
     3961 gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc aatctcgccg
     4021 cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga cgaccagcgg
     4081 ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg taccggattt
     4141 gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg tatcgcccac
     4201 gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg tgcggtagaa
     4261 agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg ctggcgcttc
     4321 catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct gcatcattgg
     4381 tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac ggctaatgcg
     4441 tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt caaccagctc
     4501 gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt gtttagtgtg
     4561 attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct gtatgcatag
     4621 cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc aatgttagac
     4681 ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa atataccttc
     4741 gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga cgaggtcgca
     4801 cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg gaatataatc
     4861 aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc gcacaattca
     4921 cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag ctgaaaagcc
     4981 agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa ataatgtagg
     5041 gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg attacccaat
     5101 tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa ccaactgaca
     5161 attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat cagattattc
     5221 accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg caaagcacca
     5281 taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt atcaatgcgc
     5341 atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg tacaacttcc
     5401 tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa ccgattgaat
     5461 aatgatgtca aaatgagttc cctctcttat ta
//
LOCUS       DAZXUK010000238         5487 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZXUK010000238 DAZXUK010000000
VERSION     DAZXUK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.238"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1630)
                     /locus_tag="ACH3L8_003445"
     CDS             complement(<1..1630)
                     /locus_tag="ACH3L8_003445"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFT4086412.1"
                     /translation="MENSRIPREHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2541)
                     /locus_tag="ACH3L8_003446"
                     /pseudo
     CDS             complement(1646..2541)
                     /locus_tag="ACH3L8_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(2541..3146)
                     /locus_tag="ACH3L8_003447"
     CDS             complement(2541..3146)
                     /locus_tag="ACH3L8_003447"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFT4086413.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5487)
                     /locus_tag="ACH3L8_003448"
     CDS             complement(3317..>5487)
                     /locus_tag="ACH3L8_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFT4086414.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1582 a         1363 c         1141 g         1401 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccta gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgtttat
//
LOCUS       DAZXUK010000239         5445 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZXUK010000239 DAZXUK010000000
VERSION     DAZXUK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.239"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACH3L8_003449"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACH3L8_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFT4086415.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPYNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="ACH3L8_003450"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="ACH3L8_003450"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFT4086416.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="ACH3L8_003451"
     CDS             complement(3736..4092)
                     /locus_tag="ACH3L8_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFT4086417.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="ACH3L8_003452"
     CDS             complement(4096..4512)
                     /locus_tag="ACH3L8_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFT4086418.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACH3L8_003453"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACH3L8_003453"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFT4086419.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1273 a         1448 c         1432 g         1292 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt atacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtt
//
LOCUS       DAZXUK010000240         5435 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZXUK010000240 DAZXUK010000000
VERSION     DAZXUK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.240"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..171
                     /locus_tag="ACH3L8_003454"
     CDS             <1..171
                     /locus_tag="ACH3L8_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4086420.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="ACH3L8_003455"
                     /gene_synonym="chbF"
                     /pseudo
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="ACH3L8_003455"
                     /gene_synonym="chbF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="ACH3L8_003456"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="ACH3L8_003456"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFT4086421.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACH3L8_003457"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACH3L8_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFT4086422.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="ACH3L8_003458"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="ACH3L8_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFT4086423.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1349 a         1197 c         1415 g         1474 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcatg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgctaat catgatgagt
     5341 tatttttgat agttcatcct gtggcgtcgc tcttaaactt atattgtaaa tgacttagat
     5401 ttaaataaga taataatttt gttaacaata tgagg
//
LOCUS       DAZXUK010000241         5434 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZXUK010000241 DAZXUK010000000
VERSION     DAZXUK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.241"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            476..1465
                     /gene="araF"
                     /locus_tag="ACH3L8_003459"
                     /pseudo
     CDS             476..1465
                     /gene="araF"
                     /locus_tag="ACH3L8_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            1535..3049
                     /gene="araG"
                     /locus_tag="ACH3L8_003460"
     CDS             1535..3049
                     /gene="araG"
                     /locus_tag="ACH3L8_003460"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFT4086424.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3064..4050
                     /gene="araH"
                     /locus_tag="ACH3L8_003461"
     CDS             3064..4050
                     /gene="araH"
                     /locus_tag="ACH3L8_003461"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFT4086425.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4217..5017
                     /gene="otsB"
                     /locus_tag="ACH3L8_003462"
     CDS             4217..5017
                     /gene="otsB"
                     /locus_tag="ACH3L8_003462"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFT4086426.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            4992..5434
                     /locus_tag="ACH3L8_003463"
                     /pseudo
     CDS             4992..5434
                     /locus_tag="ACH3L8_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
BASE COUNT         1338 a         1265 c         1461 g         1370 t
ORIGIN      
        1 ttgcgagatg gctctcatta tacgtgttct gttaattaat gagcacagtg ataattaatg
       61 gcgaaatgaa tgacgcgcat tttccacatc tttgagttgt ggttattaca ccatttcaaa
      121 aactcaacgc caggtaatgc ggcctattga ctggttaaaa agaagacatc ccgcatgggt
      181 accgaagaca acaaggattt ccaggctaat cttatggatt aatctgctgt gcattcaaca
      241 atttgtctga caaattggct tttccttatg tcttttcccg ctaaatttat gcacgttctc
      301 actgtaattc tgcgatgtga tattgctctc ctatggagaa ttaatttctc gctaaaacta
      361 tgtcaacacg gtcacttatc ttttagataa aaggtaatgc tttgttttcc gattaattta
      421 acgaatgtca ttcgtttttg ccctacacaa aacgacacta aagctggaga gaaccatgca
      481 caaatttact aaagccctgg cagccattgg tctggcagcc gttatgtcac aatccgctat
      541 ggcggagaac ctgaagctcg gttttctggt gaagcaaccg gaagagccgt ggttccagac
      601 cgaatggaag tttgccgata aagccgggaa ggatttaggg tttgaggtta ttaagattgc
      661 cgtgccggat ggcgaaaaaa cattgaacgc gatcgacagc ctggcagcca gtggcgcaaa
      721 aggtttcgtt atttgtactc cggacccgaa actcggctct gccatcgtcg cgaaagcgcg
      781 tggctacgat atgaaagtca ttgcagtgga tgaccagttt gttaacgcca aaggtaagcc
      841 aatggatacc gttcctctgg tgatgatggc ggcgactaaa attggcgaac gtcagggcta
      901 ggaactgtat aaagagatgc agaaacgtgg ctgggatgtc aaagaaagcg cagtgatggc
      961 gattaccgcc aacgaactgg ataccgcccg ccgccgtaca acgggttcta tggacgcact
     1021 gaaagctgca ggattcccgg aaaaacaaat ttatcaggta cctaccaaat ctaacgacat
     1081 cccgggggca tttgacgctg ccaactcaat gctggttcaa catccggaag ttaaacattg
     1141 gctgatcgtc ggtatgaacg acagcaccgt acttggcggc gtacgtgcaa ccgaaggtca
     1201 gggctttaaa gcagccgata tcatcggtat tggtattaac ggtgtggatg cagtaagcga
     1261 actgtctaaa gcacaggcaa ccggcttcta cggttccctg ctgccaagcc cggacgtaca
     1321 tggctataaa tccagcgaaa tgctttacaa ctgggtagca aaagacgttg aaacgccaaa
     1381 atttaccgaa gttacagacg tggtactgat cacgcgtgac aactttaaag aagaactgga
     1441 gaaaaaaggt ttaggcggta agtaatttgc cggaaaaatt cccctctgca tgatgcagag
     1501 ggggtgtgaa cgaccagtga ttcacggaga cgttatgcaa cagtctaccc cgtatctctc
     1561 atttcgcggc atcggtaaaa cgtttcccgg cgttaaggcg ctgacggata ttagttttga
     1621 ctgctatgcc ggtcaggttc atgcgttgat gggtgaaaat ggcgcaggaa aatcaactct
     1681 cttaaaaatc ctcagcggca actatgcgcc aaccacgggt tctgtagtga ttaatgggca
     1741 ggaaatgtcc ttttccgaca cgaccgcagc acttaacgcg ggtgtggcga ttatttacca
     1801 ggaactgcat ctcgtgccgg aaatgaccgt cgcggaaaac atctatctcg gccagctgcc
     1861 gcataaaggc ggcattgtga atcgctcatt gctgaattat gaggcgggtt tacaacttaa
     1921 acatcttggt atggatattg acccggacac gccgctgaaa tatctcgcca ttggtcagtg
     1981 gcagatggtt gaaatcgcca aagcgctggc gcgtaacgct aaaattatcg cctttgatga
     2041 gccaaccagc tccctttctg cccgcgaaat cggcaatctt ttccgcgtta ttcgtgaact
     2101 gcgaaaagag gggcgggtga tcttatacgt ttctcaccgt atggaagaaa tatttgccct
     2161 cagcgatgcc atcactgtct ttaaagatgg acgttatgtc aaaaccttta ccgatatgca
     2221 gcaggttgac cacgacgcgc tggtgcaggc gatggtcggg cgcgacattg gcgatatcta
     2281 cggctggcaa cctcgtagtt atggcgagga gcgcctgcgt cttgatgctg tgaaagcact
     2341 aggcgtgcgt acgccaataa gtctggcggt tcgcagtggt gaaattgttg ggctgtttgg
     2401 tctggtaggg gcggggcgta gcgaattaat gaaaggcttg tttggcggga cgcaaatcac
     2461 cgccggtcag gtttatatcg accaacagcc gatcgatatt cgtaaaccga gccacgccat
     2521 tgccgcaggc atgatgctct gcccggaagc tcgcaaagcg gaaggcatta ttcccgtgca
     2581 ctccgttcgc gacaatatca acatcagtgc cagacgcaaa catgtgctcg gcggttgtgt
     2641 aatcaacaac agttgggaag aaaacaatgc cgatcagcac attcgttcgc tcaacatcaa
     2701 aacgccgggc gcggagcaac tgatcatgaa tctctcaggc ggaaatcagc aaaaagccat
     2761 tctgggccgc tggttatcgg aagagatgaa ggtcattttg ctggatgaac ctacgcgcgg
     2821 cattgatgtt ggcgctaagc acgaaatata taacgtaatt tatgcgctgg cggcgcaggg
     2881 cgtggcggtg ctgtttgcct ccagcgactt acctgaagtc ctcggcgttg ccgaccggat
     2941 tgtggtgatg cgggaaggcg aaatcgccgg tgaattgtta cacgagcagg cagatgagcg
     3001 tcaggcactg agccttgcga tgcctaaagt cagccaggca gttgcctgag taaggagagt
     3061 atgatgtctt ctgtttctac atcggggtct ggcgcaccta agtcgtcatt cagcttcggg
     3121 cgtatctggg atcagtacgg catgctggtg gtgtttgcgg tgctctttat cgcctgtgcc
     3181 atttttgtcc caaattttgc caccttcatt aatatgaaag ggttgggcct ggcaatttcc
     3241 atgtcgggga tggtggcgtg tggcatgttg ttctgccttg cttccggtga ctttgacctt
     3301 tctgtcgcct ccgtaattgc ctgtgcgggt gtcaccacgg cggtggttat caacctgact
     3361 gaaagcctgt ggattggcgt ggcagcgggt ttgttgctgg gcgttctctg tggcctggtc
     3421 aatggctttg ttatcgccaa actgaaaata aatgctctga tcacgacact ggcaacgatg
     3481 cagattgttc gaggtctggc gtacatcatt tcagacggta aagcggtcgg tatcgaagat
     3541 gaaagcttct ttgcccttgg ttacgctaac tggttcggtc tgcctgcgcc aatctggctc
     3601 accgtcgcgt gtctgattat ctttggtttg ctgctgaata aaaccacctt tggtcgtaat
     3661 accctggcga ttggcgggaa cgaagaggct gcgcgtctgg cgggtgtacc ggttgttcgc
     3721 accaaaatta ttatctttgt tctctctggc ctggtatctg cgatagccgg aattattctg
     3781 gcttcacgta tgactagtgg gcagccaatg acgtcgattg gttatgagct tattgttatc
     3841 tccgcctgcg ttttaggtgg cgtttctctg aaaggtggca tcggaaaaat ctcatatgtg
     3901 gtggcgggta tcttaatttt aggcaccgtg gaaaacgcca tgaacctgct taatatttct
     3961 cctttcgcgc agtacgtggt tcgcggctta atcctgctgg cagcggtgat cttcgaccgt
     4021 tacaagcaaa aagcgaagcg cactgtctga tgcttttttc cacaacaact taacgttttt
     4081 tcccaccaca gccagccgcc acaacggttg gctgttcttc gttgcaaatg gcgacctccg
     4141 tcacactgtc tatacttaca tgtctgtaaa gcgcggtctg cgcaacacaa taagaaaaga
     4201 gaaggaggag agccgggtga cagaaccgtt aaccgaaacc cctgaactat ccgcgaaata
     4261 tgcctggttt tttgatcttg atggaacgct ggcggaaatc aaaccgcatc ccgatcaggt
     4321 cgtcgtgcct gacaatattc tgcaaggact acagctactg gcaaccgcaa gtgatggtgc
     4381 attggcattg atatcagggc gctcaatggt ggagcttgac gcactggcaa aaccttatcg
     4441 cttcccgtta gcgggcgtgc atggggcgga gcgccgtgac atcaatggta aaacacatat
     4501 cgttcatctg ccggatgcga ttgcgcgtga tattagcgtg caactgcata cggtcatcgc
     4561 tcagtatccc ggcgcggagc tggaggcgaa agggatggct tttgcgctgc attatcgtca
     4621 ggctccgcag catgaagacg cattaatgac attagcgcaa cgtattactc agatctggcc
     4681 acaaatggcg ttacagcagg gaaagtgtgt tgtcgagatc aaaccgagag gtaccagtaa
     4741 aggtgaagcg attgcagcct ttatgcagga agctcctttt atcgggcgaa cgcccgtatt
     4801 tctgggcgat gatttaaccg atgaatctgg cttcgcagtc gttaaccgac tgggcggaat
     4861 gtcagtaaaa attggcacag gtgcaactca ggcatcatgg cgactggcgg gtgtgccgga
     4921 tgtctggagc tggcttgaaa tgataaccgc cgcattacaa caaaaaagag aaaataacag
     4981 gagtgatgac tatgagtcgt ttagtcgtag tatctaaccg gattgcacca ccagacgagc
     5041 acgccgccag tgccggtggc cttgccgttg gcatactggg ggcactgaaa gccgcaggcg
     5101 gactgtggtt tggctggagt ggtgaaacag ggaatgagga tcagccgcta aaaaaggtaa
     5161 cattacgtgg gcctctttta acctcagcga acaggacctt gacgaatact acaaccaatt
     5221 ctccaatgcc gttctctggc ccgcttttca ttatcggctc gatctggtgc aatttcagcg
     5281 tcctgcctgg gacggctatc tacgcgtaaa tgcgttgctg gcagataaat tactgccgct
     5341 gttgcaagac gatgacatta tctggatcca cgattatcac ctgttgccct ttgcgcatga
     5401 attacgcaaa cgtggggtga ataatcgcat tggt
//
LOCUS       DAZXUK010000242         5410 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZXUK010000242 DAZXUK010000000
VERSION     DAZXUK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.242"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(561..937)
                     /gene="rnpB"
                     /locus_tag="ACH3L8_003464"
     ncRNA           complement(561..937)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACH3L8_003464"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(970..2115)
                     /gene="garK"
                     /locus_tag="ACH3L8_003465"
     CDS             complement(970..2115)
                     /gene="garK"
                     /locus_tag="ACH3L8_003465"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFT4086427.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(2212..3102)
                     /gene="garR"
                     /locus_tag="ACH3L8_003466"
     CDS             complement(2212..3102)
                     /gene="garR"
                     /locus_tag="ACH3L8_003466"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFT4086428.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(3132..3902)
                     /gene="garL"
                     /locus_tag="ACH3L8_003467"
                     /pseudo
     CDS             complement(3132..3902)
                     /gene="garL"
                     /locus_tag="ACH3L8_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3918..5252)
                     /gene="garP"
                     /locus_tag="ACH3L8_003468"
     CDS             complement(3918..5252)
                     /gene="garP"
                     /locus_tag="ACH3L8_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFT4086429.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1316 a         1525 c         1285 g         1284 t
ORIGIN      
        1 ttaccctgtg caccaaaagc aaaaacttat cgacgcgggt ttgaaactga tggcaaccgc
       61 aaaaatgcct gatgcgctac gcttatcagg cctacgccat ctctgcaata tattgaattt
      121 gcgtgctttt gtaggcagga taaggcgttc acgcagcatc cggcatgaat aaagcgcact
      181 caacaatctg aaacccgccg gaacggttta ttacgtacat caggtgaaac tgaccgataa
      241 gccgctttct tttgggtata gtgtcgtgga cagtcattca tctttctgcc cctccaaaag
      301 caaaaacccg ccgaagcggg tttttacgta tatcaggtga aactgaccga taagccgctt
      361 tcttttgggt atagcgtcgt ggacagtcat tcatctttct gcccctccaa aagcaaaaac
      421 ccgccgaagc gggtttttac gtacatcagg tgaaactgac cgataagccg ctttcttttg
      481 ggtatagcgt cgtggacagt cattcatctt tctgcccctc caaaagcaaa aacccgccga
      541 agcgggtttt tacgtaaaac aggtgaaact gaccgataag ccgggttctg tcgtggacag
      601 tcattcatct aggccagcaa tcgctcactg gctcaagcag cctacccggg ttcagtacgg
      661 gccgtacctt atgaacccct atttggcctt gctccgggtg gagtttaccg tgccacggac
      721 tgttaccagc cgcgcggtgc gctcttaccg caccctttca cccttacctg atcccgcttg
      781 cacgggccat cggcggtttg ctctctgttg cactggtcgt gggtttcccc cccaggcgtt
      841 acctggcacc ctgccctatg gagcccggac tttcctcccc tccacccgtc tcccccgaag
      901 aggacgacga cgaagcggcg actgtctggt cagcttcggc gcgcagtata gagggtttgc
      961 gcgcccttgt caccccgtgt tgcgcattcc aatcgccagt gtcgcggcaa tattacgtga
     1021 agcacggcag atattgtcat aagccccgcg gaatgcttcg tccaacgtac ctatgctggt
     1081 caatacgctg aagaccgcat caatgccatg ctgatgtaca acgccaacat catcggtcag
     1141 gctacccgca atgccaatca ccggtttatg gtacttcttc gccacgtttg cgacgccaat
     1201 cggtaccttg ccgtgaatac tctggctgtc aatacgccct tcaccggtga tcaccagcgt
     1261 acaatcgtga atatgttcct ccagattcag cgccgtagtg acgatttcaa taccactttt
     1321 cagttccgca ccaagaaacg ccatcagcgc cgcacccata ccgcccgcag ctcctgcgcc
     1381 ggggacatct tttacatcaa catgcagcgc ttttttaatg acatcggcat agtgagagag
     1441 gttattgtcc agctcaacaa tcatcgcttc actggctccc ttttgtgggc caaagatgcg
     1501 cgatgcgccg ttatcgccca ccagcggatt ggtgacatca caagcgacgc gaatgacgca
     1561 atcttttaag cgcggatcga ggccggaaat atcaatatca ttcagagtat taagactacc
     1621 gccgccaaaa ccaatttcat tgccgttggc gtcgcataat ttcgccccca gcgcctgtac
     1681 catgcctgcg ccgccatcat ttgtagcgct accgccaatg ccgataataa tgtttgtcgc
     1741 gccgctctcc agcgcctgca ggattaactc gcctgtgccg cgtgaagtgg tcacgagtgg
     1801 atcgcgtttt tccgcaggta ccagctccag cccactggcc gccgccattt caataaacgc
     1861 ggttttgcca tcgccggaga tcccccaact ggcattcact ttctcgccca gcggccctgt
     1921 aacccaggcg tgacgttcag aaccctgggt ggctgcaatc atcgcttcca ccgttccttc
     1981 gccaccgtcg gcaaccggaa tagaaacgta ctgtgcatca ggaaaaattt cccgaaatcc
     2041 tttttctatc gcctgcgcaa cctcgctggc agataaactt tctttataag agtctggggc
     2101 gattacgatt ttcataccta tgcctgttac cacatgacgc cggagggcgt ttctcttatt
     2161 cggcctggat tccaggcccg gattgcaata cgccatccgg gcacgacgtc attaacgagt
     2221 aacttcgact ttcgccagtt tttcgtagta gcacaccagg gcgctatgat ccgccgttcc
     2281 taaaccatct gctcgcagtg cctgcatcat ctccataacc gcagctgtga gcggcagttg
     2341 cgcgccgacg ccgtgggaag tatccagcgc atttgccaga tccttaatat gcagatcaat
     2401 acggaagccc ggcttgaagt tgcggtccat caccatcggc gctttggcat ccagcacggt
     2461 actgcccgcc agtccaccgc gaattgcctg atacaccagg tccgggttaa cgcccgcttt
     2521 agttgccagc gttaacgctt ctgacatcgc ggcaatattc agcgccacaa tgacctgatt
     2581 cgccagtttg gtgacgttac ctgcaccaat tgccccggta tgcaccacgg aacccgccat
     2641 tgctttcatc aaatcatagt atttgtcgaa aatagccttg tcgccgccca ccatcactga
     2701 cagcgtaccg tcgatggctt tcggttcacc gccgctcacc ggagcatcca gcatatcaat
     2761 gcctttcgct ttcagcgctt cgctgatttc acggcttgcc agcggtgcga tagaactcat
     2821 atcgatcaat accgtacctg gcttcgcgcc ttcaataatg ccattctcac ccagcgccac
     2881 ctctttcaca tgaggggagt ttggcagcat ggttatgatg acgtcgcact gttcagcgat
     2941 cgctttagcc gtagaggctg tttctgcacc tgcagcaatc acgtcagcaa tagcttctgg
     3001 gttacggtca gcaaccacca gcgagtaacc tgctttcaga aggtttttac tcattggttt
     3061 acccataatc cccaggccaa taaaaccaac tttcatagtc atatcaatca tctctcttgt
     3121 tgcggtggtg gttatttttt aaaggtatca gccagtttat gagtggcaga gcggaagacg
     3181 ccgagatcgc tgccgacagc cacaaacgtc gcgccccatt ccagataacg acgcgcatcg
     3241 gcttcgaccg gcgcgaggat accgctgggt ttgccgtgcg cgctggcacg gttaaaaatg
     3301 tgctgaattg ctttttgtac atccgggtgt gatgcattgc cgagatggcc taatgccgcg
     3361 gccagatcgc tggggccgac gaagatgccg tctacgcctt cggtagcggc aatggcatcg
     3421 acgttatcta cgccctgctg actttctatc tggaccagaa tagtgatgtt cttgttcgac
     3481 tgagcgaaat aatccgccac ggtgccaaac atattggcgc ggtgagaaac ggagacgccg
     3541 cgaatgcctt ccggtgggta acgggttgat gccaccgcct gctctgcttc ctcttttgtt
     3601 tctacaaaag gaatcaggaa gttatagaaa ccgatatcca gaagacgctt aataatttcc
     3661 ggctcgttgg tcggcactca cactactggc gcgctggcgc tgcctttcaa ggccattaac
     3721 tgcggaataa acgtggagat atcgtttggc gcatgttcgc catccagcac cagccagtca
     3781 aacccagcca aaccaagaac ttcagtgcta atcgggttag agagtgctga ccagcaacca
     3841 atttgtacct gtttcgcagc cagtgcggct ttgaatttat tcgggaaaac atcgttattc
     3901 atcgcttata cctttgctta tttctgcaat tccatacgtt taatgtcgcc aactacgaag
     3961 aggtagcaga ccatcgccat cagcgctgaa catcccacga aaaccagtgc tgcattgaag
     4021 gagtgcagtt cacttaccag gtagccaatc accagtggag tgacaatgga ggcaacattg
     4081 ccaaagacgt taaagacgcc gccacagagg ccaacaatct ctttcggcgc ggtgtcagaa
     4141 atcaccggcc agcccagagc accaaatcct ttgccaaaaa aagccagcgc catcagcatg
     4201 accaccagcg tggtgttgtt ggtgtagtta cataagatga tggtggaagc cagcaacatt
     4261 cccagcacaa tcggtagctt acgtgccagg gtcagggata aaccgcgttt gatcagataa
     4321 tccgagaaga cacctcccag cacgccgccc gcaaaaccac acagtgccgg aatcgaggcg
     4381 accagaccca ctttcagaat cgacatgcct ttttcctgca ccagataaat cgggaaccag
     4441 gtgaggaaga accaggtgat ggtgttgata aaatattgtc cgaaaaatac gcccaacatc
     4501 atgcggttag agagcaattg cttgatgtaa tgcagtttgg gaccgcttgc tgccgcgctg
     4561 cccggctttt tgtggtccat atcgaccacc gcgccatttt cagagataaa cttcagctct
     4621 tcctcagaca tacgtgggtg atctgtcggg ttatgaatca acttgatcca cagcgccgtc
     4681 agcacaaaac aaatcacccc cataacggta aagacgtgct cccatcccca ggcgaaagtc
     4741 agccagccaa gcagaggcga aaagagcgcc agcgagaaat attgtgccga gttaaagatg
     4801 gcggaggcag taccacgttc tttcgtcggg aaccaggcgg cgacaatacg ggcgttcgcc
     4861 gggaaggatg gagcttccga gaatcccagc ataaagcgca taaagaacat ggagatccct
     4921 gcccaagcca gcgggaacat atcaacaaag ccttgcagga aggtgaacag cgaccagaaa
     4981 aagaggctgt aggtgtaaac ttttttcgag ccaaacttat caagcaacca gccgccgggg
     5041 atttgcatca gcaagtaggc ccagccaaaa gcggagaaga tgtaacccat cgaaaccgca
     5101 cttaactgca actcttttgc cacttcggta ccagcaatag acagcgttgc acgatcggcg
     5161 tagttaacgg cggtaacaat aaaaataatc agtaatatta aatagcgggt atgcacacct
     5221 ttcctttttt cgtcaacggt gtccagaatc attttattta cctcgggtac ttatgctgat
     5281 ttttattatt atggggaagg tgttatttat aagtttcatt tatgccgtaa cgacaatgaa
     5341 ctcgggaatt agtataagca gcgcgagaat aataatcatt gtgcaaatgc taatttaatt
     5401 aatacttttt
//
LOCUS       DAZXUK010000243         5398 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZXUK010000243 DAZXUK010000000
VERSION     DAZXUK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.243"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..497
                     /locus_tag="ACH3L8_003469"
     CDS             <1..497
                     /locus_tag="ACH3L8_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HFT4086430.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="ACH3L8_003470"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="ACH3L8_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="ACH3L8_003471"
     CDS             2500..3114
                     /locus_tag="ACH3L8_003471"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFT4086431.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="ACH3L8_003472"
     CDS             3114..3443
                     /locus_tag="ACH3L8_003472"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFT4086432.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="ACH3L8_003473"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="ACH3L8_003473"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFT4086433.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="ACH3L8_003474"
     CDS             4529..4840
                     /locus_tag="ACH3L8_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFT4086434.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..5373
                     /locus_tag="ACH3L8_003475"
                     /pseudo
     CDS             4824..5373
                     /locus_tag="ACH3L8_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1177 a         1344 c         1425 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DAZXUK010000244         5368 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZXUK010000244 DAZXUK010000000
VERSION     DAZXUK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.244"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(38..2107)
                     /gene="glyS"
                     /locus_tag="ACH3L8_003476"
     CDS             complement(38..2107)
                     /gene="glyS"
                     /locus_tag="ACH3L8_003476"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFT4086435.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2117..3028)
                     /gene="glyQ"
                     /locus_tag="ACH3L8_003477"
     CDS             complement(2117..3028)
                     /gene="glyQ"
                     /locus_tag="ACH3L8_003477"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFT4086436.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3123..3422)
                     /locus_tag="ACH3L8_003478"
     CDS             complement(3123..3422)
                     /locus_tag="ACH3L8_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFT4086437.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3597..4592
                     /gene="wecH"
                     /locus_tag="ACH3L8_003479"
     CDS             3597..4592
                     /gene="wecH"
                     /locus_tag="ACH3L8_003479"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFT4086438.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4634..5071)
                     /gene="yiaA"
                     /locus_tag="ACH3L8_003480"
     CDS             complement(4634..5071)
                     /gene="yiaA"
                     /locus_tag="ACH3L8_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFT4086439.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5117..>5368)
                     /locus_tag="ACH3L8_003481"
     CDS             complement(5117..>5368)
                     /locus_tag="ACH3L8_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086440.1"
                     /translation="SGKGYFLGVLMTATFGNYAYLRAEKLGQLDNFFTHICQLVALIT
                     IGLLFIGVLNAPINAYEMVIYPIAFFVCLFGQMRLFRSV"
BASE COUNT         1274 a         1389 c         1337 g         1368 t
ORIGIN      
        1 agcctgccag atggcaggct ttttaataac ggcgttatta ctgcaacagc gaaatatccg
       61 caacgcgcag gaacagttcg cgcagtttct ccagcatggt cagacggttg agacgcaatt
      121 ctttgtcatc aaccatgacc atcactttat cgaagaaagc atcaaccggc tcacgcagct
      181 ctgccagttc gaccagcgca tcctggtaac gaccttccgc aaagtacggc tccagcttgt
      241 cacgtagcac cacaacctgc atcgccagtt taatttcttc cggctctttc agggtagagg
      301 cattcacgcg atcgctcagc acttcgtcag atttcgccag gatgttagat acacgtttgt
      361 tcgccgccgc cagtgctgca gctgcttcca gggtacggaa gtgcgatacc gctttcatgc
      421 gggcatcgaa atcagccgga cgagtcggac gacgcgccag taccgcctgg atggtgtcaa
      481 cggtataacc ttcgtcctga taccaggcgc ggaagcgacc gagcataaag tcgataacat
      541 catcaactac gttggcatta gtcagcttat cgccatacag acgcaccgct tcttcggtca
      601 gcgtttgcag atcaaggttg aggttcttct caacgataat tcgcagcacg ccaagtgcgg
      661 cacgacgcag cgcaaacggg tctttgtcgc ctttcggatg ctgaccgata ccgaagatac
      721 ccgccagagt atccatctta tcagcaatcg ccagcgcaca agctaccggg ttggacggca
      781 ggtcatcacc ggcaaagcgc ggctgatact gctcattcag cgcgacggct acgtcttccg
      841 cttcgccatc gtgacgcgcg tagtgcatcc ccataacgcc ttgggtgtcg gtgaactcga
      901 agaccatgtt ggtcatcagg tcacacttag acagcaggcc cgcacgggtt gcgtggttaa
      961 cgtcagcgcc aatctgttcg gcgatccagc cagccagcgc ctggatgcgg tcagtcttgt
     1021 cacgcagcgt acccagctgt tgctggaaca gcacggtttg caggcgcggc aggttatctt
     1081 ccagacgttt tttgcggtcg gtgttgaaga agaactcggc atccgccaga cgtggacgaa
     1141 cgactttctc gttaccagag ataatttgct gcggatcttt cgattcgatg ttggcaacga
     1201 agataaagtt cggcagcagt ttgccgtcgt tcgcatacac cgggaagtac ttctggtcac
     1261 ctttcatggt gtaaaccagg gcttcagccg gcaccgcgag gaatttctct tcgaattttg
     1321 cggtcagaac gaccggccac tccaccagcg aagccacttc ttccagcagg ctttcgctta
     1381 agtcagcgtt accgccaatc ttacgcgctg cttcttcggc atcggcttta atcttcgctt
     1441 tacgttcttc gtaatcggcg atgactttcc cgcgctcacg cagaatttcc ggatactgat
     1501 cggcgttgtc gatggtgaat tccggttcgc ccataaagcg gtggccgcga atcacgcgat
     1561 cggactgaat gcccagaatg gttgccggaa tgactttgtc gcccagcagc agggtcacgg
     1621 tgtgcaccgg acgcacgaag tgcacgtcgc ttgcacccca gcgcatcagt ttcgggatcg
     1681 gcagtttcgc cagcgaagtc gcaaccatat tcggcagcag tgcttcggtg ctttcgccct
     1741 tcacatgggc gcgatacagc agccattcgc ctttatcggt agtcagacgc tcagcctggt
     1801 caacggtaat accacaacca cgcgcccaac cttctgccgc tttgctcggt tttccttcag
     1861 cgtcgaacgc ctgggcaatc gccgggccgc gtttttcgat ttcacgatct ggttgtgctt
     1921 ccgccaggtt agctactttc agcgccagac gacgcggagc agcaaaccat tgaacggtgc
     1981 cgtgtgcgag gcctgcgtta tccagctccg cagtaaagtt cgcagcaaag gactctgcca
     2041 ggctgcgcag tgcttttggt ggcagctctt cagtgccgat ttccaccaga aaagttttct
     2101 cagacatagc cgcctcttac ttatctttgt tgcacatcgg gaagccgagg gcttcacggg
     2161 aagcgtaata tgcttctgcc actgctttgg tcagggtgcg aatgcgcaga atgtagcgct
     2221 gacgctcggt gacggagatg gctttacgcg catccagcag gttgaagctg tgggcggctt
     2281 tcagaatacg ctcgtaggct ggcagcggca gcggattttc cagcgccagc agctgctgcg
     2341 cttctttctc gtactgctca aagcaggtga acaggaagtc cacatccgcg tattcgaagt
     2401 tgtaagtgga ctgctccact tcgttctgat ggaacacgtc gccgtaggtg gttttaccca
     2461 gcgggccgtc gctccagact aggtcgtaaa cgctgtctac gccctgaatg tacatggcca
     2521 gacgttccag accgtaggtg atctcgccgg taaccggttt acactccaga ccaccaacct
     2581 gctggaagta agtgaactgc gtcacttcca tgccgtttag ccacacttcc cagcccagtc
     2641 cccaggcccc cagagtcggg ttttcccagt tatcttccac gaaacggatg tcgtgaatag
     2701 tcgggtccat gcccagctct ttcagagaac cgaggtacag ctcctgaata ttgtccggcg
     2761 atggcttaat gaccacctgg aactgatagt agtgctgtaa acggttgggg ttttcgccgt
     2821 agcgaccatc ggtcgggcga cgagaaggct gaacataagc agccgccatc ggttctggcc
     2881 ccagcgcgcg cagacaggtc attgggtgag aggttcccgc gccgacttcc atgtccaatg
     2941 gttgaacaat ggtgcagccc tggcgagccc agtaatcctg taaggtcagg atcaagccct
     3001 ggaaggtcct ggtatcaaac ttttgcatat tatttcgtgc tggatacgtg tggatttaaa
     3061 ggaagggatc agtatacccg ctggatggaa gatatacagt acgaaacggg aaaaagcagg
     3121 gcttaacgca tggaaagatg caaaaaatgg ccatctgcgt caaaagaaca gacaaactgc
     3181 tctttacgga aggtataacc gcgcatttca tagcttccct ggaactgttc gaaggcggtg
     3241 acgtcgattt tttgctcgcc ggtgttataa cgttttgccg cctgatcctt gcataacgct
     3301 tccatattca acgaacgcag aggatcaacc cttacccgtt gtgctttctg cacgggtgac
     3361 ggtgtagaac aacccactag caccataagc gccattgccg gaaagaaagc gttcatcatc
     3421 attaaattac cgtctggttt gcaggtgagt cttattattt atatcggtat agtggctgat
     3481 atcttacgaa tttccttaag cctctatagg ctgtagtgat aaaactcaga ttattccagt
     3541 gcgccaacca aatgatttat cggtcagact ttttttaacc agatacagag acacagatgc
     3601 agcccaaaat ttactggatt gataacctgc gagggatagc gtgtttaatg gtggtgatga
     3661 ttcacaccac tacctggtat gtgaccaatg ctcatagtgt tagccccgtc acatgggata
     3721 tcgccaatgt tctgaattct gcctctcgtg tcagcgtgcc gctatttttc atgatttccg
     3781 gctatctctt ttttggcgaa cgcagcgccc agccgcgcca tttcttgcgt atcggcttat
     3841 gtctgatttt ttatagcgca atcgcactgc tctacattgc gctgtttacc tccatcaata
     3901 tggagttagc gctgaaaaac ctgctgcaaa agccagtgtt ttaccacttg tggtttttct
     3961 tcgcgattgc ggtgatttat ctggtttcac cgctgattca ggtgaagaac gtcggcggaa
     4021 aaatgttgct ggtgctaatg gtggtgattg gcattatcgc taacccaaac acagtgccgc
     4081 agaaaattga cggttttgaa tggctgccaa ttaacttata tatcaatggc gatacttttt
     4141 actacattct gtatggcatg ttgggccgcg ctatagggat gatggacaca cagcataaag
     4201 cactgtcgtg ggtgagcgcc gcgctgtttg cgacgggggt ttttattatc tctcgcggga
     4261 cattatatga attgcagtgg cgcggaaatt ttgccgatac ctggtatctt tactgtgggc
     4321 cgatggtttt tatctgcgca atcgcgctat tgactctggt taaaaacacg ctggatacgc
     4381 gtaccattcg cggacttggc ttaatctccc gccattcatt gggtatatac ggattccacg
     4441 ccttgattat ccatgcgctg cgcacccggg gaattgagct taaaaattgg ccaatactgg
     4501 atattatttg gatcttttgc gcgacgttgg cagcgagttt gttactttct atgctggtac
     4561 aacgaatcga cagaaacaga ttagtgagtt aagtaaaagc ccgttcacat tggactgacc
     4621 gggcttacgt gaattattcg ctgtattctt cctgggtaac ctgtgtttct tttggcgggt
     4681 ttattccggc atcacgaata ttcttctgca ccgctactgc accaaacaag cttaagaaga
     4741 aagccagtcc ataaaaacct ttttcactga gtaataatgt cgcattccac agacctacca
     4801 tcagtagagc aacagagata ataaagacag tcaggcaggt catataataa atggaagtgg
     4861 tcggtatgcc ttcatacttg tcccgaacgg tcttttgata agacgcagca gaaaacagtc
     4921 ctaacaccag tacggcaaaa taataccctt tttcatttaa ctgcatctct gcattccata
     4981 gccctaacag gtaggtaacg ataccaccaa cgagagctat ccatgacaca atactaaagg
     5041 ccggtgaata ggttgatatt ttgttgtcca tcacagtatt cctttcattc ctgaatgtgt
     5101 aagagcttta tgttgctcat accgagcgaa acaaacgcat ttgaccaaac aagcagacaa
     5161 aaaaggcgat ggggtagatc accatttcat aagcattgat aggtgcattt aaaacaccga
     5221 taaacaagag accgatagtg attaacgcaa ctaactggca gatatgggta aaaaaattat
     5281 ccagttgccc gagtttttct gcgcgaagat atgcatagtt accaaaagtt gctgtcatta
     5341 acacgccaag aaaatagcct tttccact
//
LOCUS       DAZXUK010000245         5350 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZXUK010000245 DAZXUK010000000
VERSION     DAZXUK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.245"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(75..557)
                     /locus_tag="ACH3L8_003482"
     CDS             complement(75..557)
                     /locus_tag="ACH3L8_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086441.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(625..2574)
                     /locus_tag="ACH3L8_003483"
     CDS             complement(625..2574)
                     /locus_tag="ACH3L8_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFT4086442.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2659..2994)
                     /locus_tag="ACH3L8_003484"
     CDS             complement(2659..2994)
                     /locus_tag="ACH3L8_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFT4086443.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2994..3350)
                     /locus_tag="ACH3L8_003485"
     CDS             complement(2994..3350)
                     /locus_tag="ACH3L8_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFT4086444.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3350..4846)
                     /locus_tag="ACH3L8_003486"
     CDS             complement(3350..4846)
                     /locus_tag="ACH3L8_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFT4086445.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4843..5004)
                     /locus_tag="ACH3L8_003487"
     CDS             complement(4843..5004)
                     /locus_tag="ACH3L8_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFT4086446.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5014..>5350)
                     /locus_tag="ACH3L8_003488"
     CDS             complement(5014..>5350)
                     /locus_tag="ACH3L8_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086447.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1276 a         1561 c         1150 g         1363 t
ORIGIN      
        1 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
       61 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      121 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      181 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      241 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      301 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      361 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      421 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      481 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      541 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      601 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      661 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      721 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      781 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
      841 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
      901 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
      961 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1021 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1081 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1141 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1201 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1261 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat
     1321 attcatcacg ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat
     1381 cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac
     1441 aaacgtgcgc acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg
     1501 caggaaggtt tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa
     1561 ctgattttct gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg
     1621 ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg
     1681 cgcaatggcc cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc
     1741 tttcggcact tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg
     1801 catatccgcc gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga
     1861 tttggtggca gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc
     1921 aatttctgac tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga
     1981 ggccacaccc gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt
     2041 cgcacttgca ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc
     2101 tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc
     2161 cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc
     2221 acgggcgata ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat
     2281 gggcagttct gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt
     2341 atccagacca tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga
     2401 tttgaccggg agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga
     2461 caggctggca aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc
     2521 cgatacctta tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca
     2581 tctcctcgtt aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc
     2641 gtcgcagctc cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga
     2701 ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat
     2761 atccagtttt gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata
     2821 tttcagtgcc acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa
     2881 acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc
     2941 gttatgcgct gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga
     3001 taccctccct cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta
     3061 gcctctccgt gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg
     3121 gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta
     3181 aacgtgccct taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg
     3241 ccgacgatgt catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat
     3301 aactgctgac catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact
     3361 cctctgaata ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat
     3421 aatccggcgg gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca
     3481 gatactgttt aaacagatcg taattttcca caataccggc acgctccatc tgacgatatg
     3541 tcgccagaag ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac
     3601 gggtaccatc atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt
     3661 tgcgaagaac atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca
     3721 cgccatacgc attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt
     3781 ccacataagc cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc
     3841 gcttaccttt cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag
     3901 gatcattccg gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag
     3961 gcgactgggt ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc
     4021 cggcaccaac cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact
     4081 ggcgcgcata actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg
     4141 aggcggcatc gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa
     4201 cagcggcggt aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa
     4261 gccctgctgg caaaatctca ccaccaccag aaccataata attcagacag accggtaact
     4321 cattaccata cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg
     4381 ccgtaaacgg cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag
     4441 tggcatcatc gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca
     4501 gggtgccgct ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc
     4561 tggcttccgg tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt
     4621 ccaccatacg cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg
     4681 gcatcagcac caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta
     4741 atgcaggcgc gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca
     4801 acggcaccag cgtattcgac ggaacagtat taaaacttat cgtcatgatt ttttagcctt
     4861 atcctgttta acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc
     4921 ttccacattt cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg
     4981 ccctttcagg ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag
     5041 atggtgctca atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact
     5101 cagcagggat aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt
     5161 gatttcatac ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag
     5221 cagggtgcca ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag
     5281 ggctttccag agttctctcc ggacatcatg aacggcgtca taggctgccc actgcccttt
     5341 ttcatcacgc
//
LOCUS       DAZXUK010000246         5339 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZXUK010000246 DAZXUK010000000
VERSION     DAZXUK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.246"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACH3L8_003489"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACH3L8_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFT4086448.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKGRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="ACH3L8_003490"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="ACH3L8_003490"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFT4086449.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="yfiH"
                     /locus_tag="ACH3L8_003491"
     CDS             1884..2615
                     /gene="yfiH"
                     /locus_tag="ACH3L8_003491"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFT4086450.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="ACH3L8_003492"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="ACH3L8_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFT4086451.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1291 c         1457 g         1281 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgacct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DAZXUK010000247         5333 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZXUK010000247 DAZXUK010000000
VERSION     DAZXUK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.247"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..487
                     /gene="lpfD-O113"
                     /locus_tag="ACH3L8_003493"
     CDS             <1..487
                     /gene="lpfD-O113"
                     /locus_tag="ACH3L8_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFT4086452.1"
                     /translation="SQPMADVYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVG
                     SAVTTHKITKTVTIECENLGILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKN
                     NKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIV
                     R"
     gene            735..1775
                     /gene="pstS"
                     /locus_tag="ACH3L8_003494"
     CDS             735..1775
                     /gene="pstS"
                     /locus_tag="ACH3L8_003494"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFT4086453.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            1862..2821
                     /gene="pstC"
                     /locus_tag="ACH3L8_003495"
     CDS             1862..2821
                     /gene="pstC"
                     /locus_tag="ACH3L8_003495"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFT4086454.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            2821..3711
                     /gene="pstA"
                     /locus_tag="ACH3L8_003496"
     CDS             2821..3711
                     /gene="pstA"
                     /locus_tag="ACH3L8_003496"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFT4086455.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            3803..4576
                     /gene="pstB"
                     /locus_tag="ACH3L8_003497"
     CDS             3803..4576
                     /gene="pstB"
                     /locus_tag="ACH3L8_003497"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFT4086456.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRVEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            4591..5316
                     /gene="phoU"
                     /locus_tag="ACH3L8_003498"
     CDS             4591..5316
                     /gene="phoU"
                     /locus_tag="ACH3L8_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFT4086457.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1286 a         1315 c         1425 g         1307 t
ORIGIN      
        1 ctcacagccg atggctgatg tttatattcg gggcgatatt acagctccgc aaagttgcga
       61 aataaataat ttaaagccag tttgttttga ttttaaagaa ataccagctg cagatttttc
      121 atctgttgtt ggaagtgcgg taacaacaca taaaattact aaaacagtca ctattgagtg
      181 tgaaaacctg gggatactga ataccgatga tatcagtacg tctttttatg ctaccgaacc
      241 caatacggac aattcaatgg tcgtaacttc aaactctaac gtggggataa aaatttacga
      301 caaaaataat aaggaaatca aagtgaacgg tggtgagttg ccaacagaca tggataaatc
      361 aacagtgtat ggtgaaaaat caggtagtgt aactttttca gctgctcctg cgagccttac
      421 tggagctcgc ccagccccgg ggcaatttac tgcaacagcg acgataactg ttgagattgt
      481 gcgctaatag gtctgacaac gattgttcat cacatcgttg tcatctcacc catttcttta
      541 tcacttttaa atgcatcatg acaaaaagat ggcatgtgga attttcatct tcacatttaa
      601 aatcagtaag ttatttggta attcctttct gtcataaaaa tgtcatattc cttatatata
      661 actgtcacct gtttgtccta ttttgcttct cgtagccaac aaacaatgct ttatgaatcc
      721 tcccaggaga cattatgaaa gttatgcgta ccaccgtcgc aactgttgtc gccgcgacct
      781 tatcgatgag tgctttctct gtgtttgcag aagcaagcct gacaggtgca ggtgcaacct
      841 tccctgcgcc ggtgtatgcc aaatgggctg acacttacca gaaagaaacc ggtaataaag
      901 ttaactacca gggtatcggt tcttccggtg gcgtaaaaca gattatcgct aataccgttg
      961 attttggtgc ctctgacgcg ccgctgtctg acgaaaaact ggctcaggaa ggtctgttcc
     1021 agttcccgac cgtgattggc ggcgtggtgc tggcggttaa cattccaggg ctgaagtctg
     1081 gcgaactggt gctggatggt aaaaccctcg gcgacatcta cctgggcaaa atcaagaagt
     1141 gggatgatga agccatcgcc aaactgaatc cgggtctgaa actgccttca caaaacattg
     1201 ctgtagtacg ccgcgcagat ggctccggga cttccttcgt cttcaccagc tacctggcga
     1261 aagtgaacga agagtggaaa aacaacgttg gtactggctc taccgtaaaa tggccgatcg
     1321 gtctgggcgg taaaggtaac gacggtatcg ccgcgttcgt tcagcgtctg ccgggtgcaa
     1381 ttggttatgt tgaatatgct tacgcgaagc agaacaacct ggcgtacacc aaactgatct
     1441 ccgctgatgg taaaccggtt agtccgaccg aagaaaactt cgctaatgca gcaaaaggtg
     1501 cagactggag caaaaccttc gctcaggatc tgaccaacca gaaaggcgaa gatgcatggc
     1561 ctattacctc taccacgttc attctgatcc acaaagatca gaagaaacca gaacaaggca
     1621 cagaggtgct gaaattcttc gactgggcgt acaaaaccgg ggctaaacag gcgaacgacc
     1681 tggattacgc cagcctgccg gatagtgtag ttgaacaggt tcgcgctgcg tggaagacca
     1741 atattaaaga cagtagcggt aagccgctgt actaataaaa ctccaggccg ggtacggtgt
     1801 tttacgccgc atccggcatt acaaaatgac tttgtaaacg cgtttaactg aagagtaact
     1861 tatggctgca accaagcccg cttttaaccc accgggtaaa aagggcgaca taattttcag
     1921 cgtgctggta aaactggcgg cgctgattgt gctattgatg ttgggtggca ttattgtctc
     1981 tctgatcatc tcctcctggc cgagcattca gaaatttggt ctggctttcc tatggaccaa
     2041 agagtgggat gcaccgaacg atatctacgg ggcgctggtg ccgatctacg gtacgttggt
     2101 gacttcgttt atcgcgctgc tgatcgccgt cccggtgagt ttcggtatcg ccctgttcct
     2161 gactgagctt gcgcctggct ggctgaaacg cccgctgggt atcgccattg agctgctggc
     2221 agccattcca agtatcgttt acggcatgtg gggcctgttt atctttgcgc cgctgttcgc
     2281 cgtttacttt caggagccgg tcggcaatat catgtcgaat atcccgattg ttggcgcgct
     2341 gttctctggc cccgcatttg gtatcggtat cctcgcggca ggcgtgatcc tcgccatcat
     2401 gattattccg tacattgcgg cggtaatgcg tgatgtgttc gaacaaaccc cggtgatgat
     2461 gaaagagtcg gcctacggta ttggctgcac cacctgggaa gttatctggc gtatcgttct
     2521 tccgttcacc aaaaatggtg ttatcggcgg catcatgctg gggctgggcc gcgcgctcgg
     2581 tgaaaccatg gcggtgacct ttatcatcgg taacacctac cagctcgaca gcgcctcgct
     2641 gtacatgccg ggcaatagta tcacctctgc gctggcgaac gaatttgcgg aagcggaatc
     2701 cggtctgcac gttgccgcac tgatggaact gggcctgatc ctgtttgtga ttaccttcat
     2761 cgtcctcgcc gcatcgaagt ttatgattat gcgcctggct aagaatgagg gggcacgcta
     2821 atggctatgg ttgaaatgca aaccactgcg gcgctggctg aatctcgccg caaaatgcag
     2881 gcgcgtcgcc gcctcaaaaa ccgtattgcg ctgacgctct cgatggcgac gatggccttc
     2941 ggcctgttct ggctgatctg gattttaatg tccaccatca ctcgcggtat cgacggtatg
     3001 tcgctggcgc tgttcactga aatgacgccg ccgcccaata cggaaggtgg tggtctggcg
     3061 aacgccctgg cgggtagcgg gctgttaatt ttgtgggcca cggtattcgg tacgccgctg
     3121 ggcattatgg cggggattta tctggcggaa tatggtcgta aatcctggct ggcagaagtg
     3181 attcgcttca ttaacgacat tctgctctcc gcgccgtcga ttgtggttgg tctgtttgtc
     3241 tacaccatcg tggtggcaca gatggagcac ttctccggct gggcgggcgt aattgcgctg
     3301 gcgctgttgc aggtgccgat cgttatccgc accactgaga acatgctgaa actggtgccg
     3361 gacagcctgc gtgaagcggc ttatgcgctg ggtacaccga agtggaagat gatctccgcg
     3421 attacgctga aagcgtcggt gtccgggatt atgaccggta tcctgctggc gattgcccgt
     3481 attgccggtg aaaccgcgcc gctgctgttt accgcgctct ccaaccagtt ctggagcacg
     3541 gacatgatgc agccgatcgc caacctgccg gtgacgatct ttaagtttgc gatgagcccg
     3601 tttgcggaat ggcaacaact ggcctgggcc ggggtattga tcattaccct gtgcgtcctg
     3661 ctgctgaaca ttctggcgcg cgttgttttt gcgaagaata aacacggtta acacttttcc
     3721 ggatgcggcg tgaacgcctt acccggccct acggtaagcc tgattagcga aaagcatcag
     3781 gtacgatgag gaaaagattg caatgagtat ggttgaaact gccccgagta aaattcaggt
     3841 tcgtaatttg aacttctact acggcaaatt ccatgccctg aaaaacatca acctggatat
     3901 cgccaaaaac caggttacgg cgtttatcgg gccgtccggc tgcggtaaat cgacgctgct
     3961 gcgtaccttc aacaaaatgt ttgaactgta cccggagcag cgtgtggaag gtgaaattct
     4021 gcttgatggc gacaacatcc tgaccaactc tcaggatatc gccctgctgc gtgcgaaagt
     4081 gggcatggtg ttccagaaac cgacgccgtt cccgatgtcc atctacgaca acatcgcctt
     4141 tggcgttcgt ctgtttgaga agctctcccg tgccgacatg gacgagcgcg tgcagtgggc
     4201 attgaccaaa gccgcattgt ggaacgaaac caaagataaa ttgcaccaga gcggttactc
     4261 tctctctggt ggtcagcaac agcgtctgtg tattgcgcgt ggtatcgcca ttcgcccgga
     4321 agtgctgctg ctcgacgaac cgtgttcggc gctcgacccc atctctaccg ggcgtattga
     4381 agagctgatc accgaactga agcaggatta caccgtggtg atcgtcaccc acaacatgca
     4441 gcaggctgcg cgttgttccg accacacggc atttatgtac ctgggcgaat tgattgagtt
     4501 cagcaacacg gacgatctgt tcaccaagcc agcgaagaaa caaacagaag actacatcac
     4561 cggtcgttac ggttgattca ggagtgcgtt atggacagtc tcaatcttaa taaacatatt
     4621 tccggccagt tcaacgccga actggaaagt atccgcacgc aggtgatgac catgggcggc
     4681 atggtggagc agcagctttc tgatgcaatc accgcgatgc ataaccagga cagcgatctg
     4741 gcgaagcgcg tgatcgaagg cgacaagaac gtcaacatga tggaagtggc gatcgatgaa
     4801 gcctgcgtgc gcattatcgc caaacgtcag ccgacggcga gcgacctgcg actggttatg
     4861 gtgatcagta aaaccattgc cgagctggag cgtattggcg acgtggcgga caaaatctgc
     4921 cgtactgcgc tggagaaatt ctcccagcag catcagccgt tgctggtaag tctggagtcg
     4981 ctgggccgtc ataccatcca gatgctgcac gacgtgctgg acgcgttcgc gcggatggac
     5041 attgacgaag cggtacgtat ttatcgtgaa gataaaaaag tcgatcagga atacgaaggt
     5101 attgttcgtc aactgatgac ctacatgatg gaagattcgc gcactattcc gagcgtactt
     5161 accgcgctgt tctgtgcgcg ttcaatcgaa cgtatcggcg accgctgcca gaatatttgt
     5221 gagtttatct tctactacgt gaagggtcag gatttccgtc atgtaggcgg tgatgagctg
     5281 gataaactgc tggcgggaaa agatagcgac aaataattca ccagacaaat ccc
//
LOCUS       DAZXUK010000248         5310 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZXUK010000248 DAZXUK010000000
VERSION     DAZXUK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.248"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            23..1225
                     /gene="fes"
                     /locus_tag="ACH3L8_003499"
     CDS             23..1225
                     /gene="fes"
                     /locus_tag="ACH3L8_003499"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFT4086458.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            1228..1446
                     /gene="ybdZ"
                     /locus_tag="ACH3L8_003500"
     CDS             1228..1446
                     /gene="ybdZ"
                     /locus_tag="ACH3L8_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFT4086459.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            1443..5288
                     /gene="entF"
                     /locus_tag="ACH3L8_003501"
     CDS             1443..5288
                     /gene="entF"
                     /locus_tag="ACH3L8_003501"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFT4086460.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         1145 a         1403 c         1555 g         1207 t
ORIGIN      
        1 acagtaaaca tggggttatg gtgtgacggc gttaaaagta ggaagtgaga gctggtggca
       61 gtcgaaacat ggcccggaat ggcagcgtct gaatgacgaa atgtttgagg tcactttctg
      121 gtggcgtgat ccccaaggtt ctgaagaata ctcgacgata aagcgcgtat gggtctacat
      181 cactggtgtg accgatcatc atcagaacag ccagccccag tcgatgcagc gaattgcagg
      241 tactgatgtc tggcagtgga cgacacaact caatgccaac tggcgcggca gctactgctt
      301 tattcccacc gaacgcgatg acattttttc tgcaccatcc cccgatcgcc tcgaattacg
      361 tgaaggctgg cgaaaactat taccccaggc aatagccgat ccgctgaatc cacaaagctg
      421 gaaaggcggg ctagggcacg ctgtttctgc actcgaaatg ccgcaagcgc ctctgcaacc
      481 gggatgggat tgtccgcaag cgccagaaat accagccaaa gaaattatct ggaaaagtga
      541 acggttgaaa aattcacgtc gtgtatggat ttttaccacc ggcgatgtaa cagcagaaga
      601 acgcccgctg gcagttttgc tcgatggcga attttgggcg caaagtatgc ccgtctggcc
      661 tgtgctgact tcgctgaccc atcgtcagca acttcctccc gccgtgtatg tgttgatcga
      721 cgctatcgac accacgcacc gcgcccacga actgccgtgt aatgcggatt tctggctggc
      781 agtacagcaa gagttattac ccctggtgaa agttattgcc ccttttagcg atcgcgccga
      841 tcgcaccgtg gtcgccgggc agagttttgg tgggctgtcc gcgctgtatg ccggactgca
      901 ctggcctgaa cgctttggct gtgtattaag ccagtcagga tcgtactggt ggccgcatcg
      961 gggcgggcag caagagggcg tgttacttga aaagctaaaa gctggtgaag ttagcgccga
     1021 aggtctgcgc attgtgctgg aagcgggtat tcgcgagccg atgatcatgc gggccaatca
     1081 ggcgctgtat gcgcaattac accctataaa agaatccatt ttctggcgtc aggttgacgg
     1141 cggacatgat gcgctttgtt ggcgcggtgg cttgatgcag gggttaatcg acctctggca
     1201 accacttttc catgacagga gttgaatatg gcattcagta atcccttcga tgatccacag
     1261 ggagcgtttt acattttgcg caatgcgcag gggcaattca gtctgtggcc gcaacaatgc
     1321 gccttaccgg caggctggga cattgtgtgt cagccgcagt cacaagagtc ctgccagcag
     1381 tggcttgaag cccactggcg tactctgaca ccggcgaatt tcacccagtt gcaggaggca
     1441 caatgagcca gcatttacct ttggtcgccg cacagcccgg catctggatg gcagaaaaac
     1501 tgtcggattt accctccgcc tggagcgtgg cgcattacgt tgagttaacc ggagaggttg
     1561 atgcgccatt actggcccgc gcggtggttg ccggattagc gcaagcagat actttgcgga
     1621 tgcgttttac ggaagataac ggcgaagtct ggcaatgggt cgatgatgcg ctgatattcg
     1681 aactgccaga aattatcgac ctgcgaacca atattgatcc gcacggtact gcgcaggcat
     1741 taatgctggc ggatttgcaa caagatttgc gcgtcgatag cggtaaacca ctggtctttc
     1801 atcaactgat acaggtggca gataaccgct ggtactggta tcagcgttat caccatttac
     1861 tggtcgatgg cttcagtttt ccggccatta cccgccagat cgccaacatt tactgcgcat
     1921 tgctgcgtgg cgaaccaacg cctgcttcgc cgtttacgcc tttcgctgat gtagtggaag
     1981 agtaccagca ataccgcgaa agcgaagcct ggcagcgtga tgcggcattc tgggtggaac
     2041 agcgtcgcca actgccaccg cccgcgtcac tttctccggc acctttagcg gggcgcagcg
     2101 cctcggcaga tattttgcgt ctgaaaatgg aatttaccga cggggaattc cgccagctgg
     2161 ctacgcaact ttcaggtgtg cagcgtaccg atttagccct tgcgctgacg gctttgtggc
     2221 tggggcgatt gtgcaatcgc atggactacg ccgccggatt tatctttatg cgtcgactgg
     2281 gctcggcggc gctgacggct accggacccg tgctcaacgt tttgccgctg ggtattcata
     2341 ttgcggcgca agaaacgctg ccggaactgg caacccgact ggcaacccga ctggcagcac
     2401 aactgaaaaa aatgcgtcgt catcaacgtt acgatgccga acaaattgtc cgtgacagcg
     2461 ggcgagcggc aggtgatgaa ccgctgtttg gtccggtact caatattaag gtatttgatt
     2521 accaactgga tattcctggt gttcaggcgc aaacccatac cctggcaacc ggtccggtta
     2581 atgaccttga actggccctg ttcccggatg aacacggtga tttgagtatt gagatcctcg
     2641 ccaataaaca gcgttacgat gagccaacgt taatccagca tgctgaacgc ctgaaaatgc
     2701 tgattgccca gttcgccgcg gatccggcgc tgttgtgcgg cgatgtcgat attatgctgc
     2761 caggtgagta tgcgcagctg gcgcagatca acgccactca ggttgagatt ccagaaacca
     2821 cgctgagcgc gctggtggca gaacaagcgg caaaaacacc ggatgctccg gcgctggcag
     2881 atgcgcgtta cctgttcagc tatcgggaaa tgcacgagca ggtggtggcg ctggcgaatc
     2941 tgctgcgtga gcgcggcgtt aaaccagggg acagcgtggc ggtggcacta ccgcgctcgg
     3001 tctttttgac cctggcactc catgcaatag ttgaagctgg cgcggcctgg ctaccgctgg
     3061 ataccggcta tccggacgat cgcctgaaaa tgatgctgga agatgcgcgt ccgtcgctgt
     3121 taattaccac cgacgatcaa ctgccgcgct ttagcgatgt tcccaattta accaaccttt
     3181 gctataacgc cccgcttacc ccccagggca gtgcgccgct gcaactttca caaccgcacc
     3241 acacggctta tatcatcttt acctctggct ccaccggcag gccgaaaggg gtaatggtcg
     3301 ggcagacggc tatcgtcaac cgcctgttgt ggattcaaaa tcattatccg ctcacaggcg
     3361 aagatgtcgt tgcccaaaaa acgccgtgca gttttgatgt ctcggtgtgg gagtttttct
     3421 ggccgtttat tgccggggct aaactggtga tggctgaacc ggaagcgcac cgcgacccgc
     3481 tcgctatgca gcaattcttt gccgaatatg gcgtaacgac cacgcacttt gtgccgtcga
     3541 tgctggcagc atttgttgcc tcgctgactc cgcaaaccgc tcgccagagt tgcgtgacgt
     3601 tgaaacaggt tttctgtagt ggtgaggcct taccggctga tttatgccgc gaatggcaac
     3661 agttaaccgg cgcgccgttg cataacctgt atggcccgac ggaagcggcg gtagatgtca
     3721 gctggtatcc ggcttttggc gaggaactgg cacaggtgcg cggcagcagt gtgccgattg
     3781 gttatccggt atggaatacg ggtctgcgta ttcttgatgc gatgatgcat ccggtgccgc
     3841 cgggtgtggc gggtgatctc tatctcaccg gtattcaact ggcgcagggg tatcttggac
     3901 gacccgatct gaccgccagc cgctttattg ccgatccttt tgctcctggt gaacggatgt
     3961 atcgtaccgg agacgttgcc cgctggctgg ataacggcgc ggtggagtac ctcgggcgca
     4021 gtgacgatca gctaaaaatt cgcgggcagc gtatcgaact gggcgaaatc gatcgcgtga
     4081 tgcaggcgct gccggatgtc aaacaagccg ttacccacgc ctgtgtgatt aaccaggcgg
     4141 cagccactgg tggtgatgcg cgtcagttgg tgggctatct ggtatcgcaa tcgggcctgc
     4201 cgttggatac cagcgcattg caggcgcagc ttcgcgaaac attgccgccg catatggtgc
     4261 cagtcgttct gctgcaactg ccgcagttgc cacttagcgc caacggcaag ctggatcgca
     4321 aagccttacc gttgcctgaa ctgaaggcac aagcgccagg gcgtgcgccg aaagcgggca
     4381 gtgaaacgat tatcgccgcg gcattcgcgt cgttgctggg ttgtgacgtg caggatgccg
     4441 atgctgattt ctttgcgctg ggcggtcatt cgctactggc aatgaaactg gcagcgcagt
     4501 taagtcgaca gtttgctcgt caggtgacgc cggggcaggt gatggtcgcg tcaaccgtcg
     4561 ccaaactggc aacgattatt gatggtgaag aggacagctc ccggcgcatg ggattcgaaa
     4621 ccattctgcc gttgcgtgaa ggtaatggcc cgacgctgtt ttgtttccat ccggcgtccg
     4681 gttttgcctg gcagtttagc gtgctctcgc gttatctcga tccacaatgg tcgattattg
     4741 gcattcagtc gccgcgccct catggaccca tgcagacagc gacaaacctg gatgaagtct
     4801 gcgaagcgca tctggcaacg ctacttgagc aacaaccgca cggcattgcg gcgcggctgc
     4861 gtgcccgtgg cgaacaggtg gcatttcttg gtttgctgga tacctggccg ccagagacgc
     4921 aaaactggca ggaaaaagag gctaatggtc tggacccgga agtgctggcg gagattaacc
     4981 gcgaacgcga ggccttcctg gcggcacagc agggaagtac ttcaacggag ttgtttacca
     5041 ccattgaagg caactacgct gatgctgtgc gcctgctgac gactgctcat agcgtaccgt
     5101 ttgatggtaa agcgacgctg tttgttgctg aacgcacgct tcaggaaggt atgagccccg
     5161 aacgcgcctg gtcgccgtgg attgcggagc tggatatcta tcgtcaggat tgtgcgcatg
     5221 tggatattat ctctccaggg gcgtttgtaa aaattgggcc gattattcgc gcaacgctaa
     5281 acaggtaaat taatattatc tataaaccca
//
LOCUS       DAZXUK010000249         5275 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZXUK010000249 DAZXUK010000000
VERSION     DAZXUK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.249"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACH3L8_003502"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACH3L8_003502"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFT4086461.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACH3L8_003503"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACH3L8_003503"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFT4086462.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3324)
                     /locus_tag="ACH3L8_003504"
                     /pseudo
     CDS             complement(2698..3324)
                     /locus_tag="ACH3L8_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3454..5214
                     /gene="ycaO"
                     /locus_tag="ACH3L8_003505"
     CDS             3454..5214
                     /gene="ycaO"
                     /locus_tag="ACH3L8_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFT4086463.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1345 a         1358 c         1263 g         1309 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatcac attaccaggc
     3061 ggtataacaa cgccagagtg ataaagacga taagtaccgg caccatcggc acatcatcat
     3121 aaaacgccac atctcccgcc gctgaaccca gcgtgagaat gattaacact tcaaacagcg
     3181 acatctgtcg cacaccgcgt cttccggtca ttttgaggaa caaaaaacca gtacaaaggt
     3241 ataaagacta cgtagtgcaa cttcgccaag gaaatcaaaa ggcactttat caaatgccat
     3301 acggtggaga tcgaacgcct tcatttttat gctcttaacg tggtttatcc gctaaaagca
     3361 tagtcaacag ttcatcggca tggcggcggt gatagcgcct ttgagataaa acccgtatta
     3421 ttgcgcgctt tccgtacgac taaagtgatt ttcatgacgc aaacatttat ccccggcaaa
     3481 gatgccgctc tggaagattc catcgctcgc ttccagcaaa aactttcaga cctcggcttt
     3541 cagattgaag aggcctcctg gctgaatccc gtgcctaacg tctggtctgt acatattcgc
     3601 gacaaagagt gcgcactgtg ttttaccaac ggtaaaggcg caaccaagaa agcggcgctg
     3661 gcttctgcac tcggtgaata tttcgagcgt ctctcaacca actacttttt tgctgatttc
     3721 tggctgggcg aaaccatcgc caacggtcca tttgtgcatt atcccaacga aaaatggttc
     3781 ccactgagcg aaaatgacga tgtgccagaa gggctgctcg atgaccgtct gcgcgcattt
     3841 tacgatccgg agaatgaatt gaccggcagt atgctgattg acctacaatc cggtaacgaa
     3901 gatcgtggta tttgcggtct gccgtttacg cgccagtccg acaatcagac cgtttatatt
     3961 ccgatgaata tcattggtaa cctgtacgtt tctaacggta tgtccgctgg caatacccgc
     4021 aacgaagcac gcgttcaggg gttgtccgaa gttttcgaac gctacgtgaa aaaccgcatt
     4081 attgctgaaa gcatcagctt gccggaaatc ccggcagacg tgctggcgcg ttacccagcg
     4141 gtggttgaag cgatcgaaac actggaaaca gaaggtttcc cgatcttcgc atatgatggt
     4201 tcccttggcg gccagtatcc ggtgatttgc gtggtactgt ttaatcctgc aaacggcacc
     4261 tgctttgcct ctttcggtgc gcatcctgat tttggcgtag cactggaacg taccgtgacc
     4321 gagctgctgc aaggtcgtgg cctgaaagat ttggatgtgt ttactccgcc aaccttcgat
     4381 gatgaagaag tcgctgaaca taccaacctc gaaacgcact ttatcgattc cagcggttta
     4441 atctcctggg acctgttcaa gcaggatgcc gattatccgt ttgtggactg gaatttctcc
     4501 ggcaccacgg aagaagagtt tgctacgctg atggctatct tcaacaaaga agataaagaa
     4561 gtttatattg ccgattacga gcatctgggc gtttatgctt gccgtattat cgtgcctggc
     4621 atgtccgata tttatccggc tgaagatctg tggctcgcga ataacagtat gggcagccat
     4681 ttacgtgaaa cgattctttc gctaccaggc agcgagtggg aaaaagaaga ttacctgaac
     4741 ctcatcgagc aactggatga agaaggtttt gatgacttta cccgcgtgcg tgagctgttg
     4801 ggtctggcga ccgggtcgga taacggttgg tacaccctgc gtatcggtga attaaaagcc
     4861 atgctggcgc tggctggtgg cgatctggaa caggctctgg tctggaccga atggacgatg
     4921 gagtttaact catcagtatt tagtccggaa cgcgccaact attatcgctg cctgcaaacg
     4981 ttgttattac tggcacagga agaagatcgc cagccgctgc aatatctgaa tgcgtttgtt
     5041 cgcatgtacg gcgcagatgc cgtagaagcc gccagtgcgg caatgagcgg cgaagcggcg
     5101 ttttacggcc tgcaaccggt agatagcgat ctgcacgcgt ttgctgcaca tcagtcgttg
     5161 ttgaaggcct acgaaaagct gcagcgcgcc aaagcagcat tctgggcaaa ataaaatcaa
     5221 atagcctacg caatgtaggc ttaatgatta gtctgagtta tattacgggg cgttt
//
LOCUS       DAZXUK010000250         5149 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZXUK010000250 DAZXUK010000000
VERSION     DAZXUK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.250"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            189..446
                     /locus_tag="ACH3L8_003506"
                     /pseudo
     CDS             189..446
                     /locus_tag="ACH3L8_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACH3L8_003507"
     CDS             complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACH3L8_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFT4086464.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACH3L8_003508"
     CDS             complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACH3L8_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFT4086465.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACH3L8_003509"
     CDS             complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACH3L8_003509"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFT4086466.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3071..3214)
                     /locus_tag="ACH3L8_003510"
                     /pseudo
     CDS             complement(3071..3214)
                     /locus_tag="ACH3L8_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3214..3430
                     /locus_tag="ACH3L8_003511"
                     /pseudo
     CDS             3214..3430
                     /locus_tag="ACH3L8_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3604..4419)
                     /locus_tag="ACH3L8_003512"
     CDS             complement(3604..4419)
                     /locus_tag="ACH3L8_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFT4086467.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4516..>5149
                     /locus_tag="ACH3L8_003513"
     CDS             4516..>5149
                     /locus_tag="ACH3L8_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFT4086468.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYT"
BASE COUNT         1374 a         1175 c         1225 g         1375 t
ORIGIN      
        1 ctattttacc gcgacaattc attcagaaca tcaatagtca gggaaggaag tagcaacatt
       61 agctaactaa tcaatgcgtt gattgtaaat ccagctaaga ggtgaggttt tcagagcaga
      121 caacggtgaa acgtcatggt attgttacgt ttaggtaaca agaaatttgt ctgcacaagg
      181 attacatcat gattatggcg aaactgaagt cagcgaaagg gaagaaattt ctctttggtt
      241 tgttggcggt tttcattatt gcggcgtcgg ttgtgactcg cgcgaccatc ggcggcgtta
      301 tagaacagta caatattccg ctgtctgagt ggacgacatc aatgtatgtg atttagtcat
      361 cgatgatttt tgtttatagc ctggtcttta ctgtgttgct ggcaatcccg ttgggaattt
      421 atttccttgg cggcgaagag cagtaagtaa aaaataggcc cgataactcg ggccttgtca
      481 gttattgaag agtcgttaat cgtcttcttc gtcatccagt tcaacgggtg tctgatactg
      541 gtcaggttta atgaccagca ggtcgcagcg aagatgatca atcacctgtt ccgccgtgtt
      601 gccgaggaat gctgctgaaa taccggtgcg tccgaccgtg cccagaacca caattcccgc
      661 ctgtaaatgc tccgccaaat caggaatcac ctcttcgggc agaccttttt ctacgtgcgt
      721 catgttttca ttaatgccga atttctgccg cagggctttc attgccagca aatgttgccc
      781 acgaatggca tcgttataaa cgctcgggtc aaattccggc agttcaatcg cgatattaat
      841 tggcgttacc ggataagcgc caaccagatg aacttcggta tggttgactt gttctgccag
      901 ttcgatcgtc tctttgacca gtttttcatt gagcgcatta tgatacggct cttcactggc
      961 gagattcacc gccaccagcg ccttgcctcc ttccggccac ggctggtctt tcaccatcca
     1021 caccgggctt gggcatttgc gtaacagatg ccagtccgtt ggcgtaaaaa tcaccgcttc
     1081 cagacggtca tgttggtgcg ccatttttag caccaaatcg tgtccgccgc tgatcacttc
     1141 ctgaatgatg gcttcgaaag gacggttatg ccagaccact ttaatttcaa tgggaacgcc
     1201 agcattgaga taatattttg cctgctcgtg gatccaggct gtacgctggc tgatgacgcc
     1261 ctgacgcata gcggtacgtt cgtccgggga gagcagggtg gtcatttcgt atgagaagtc
     1321 atagatcggc aaaaaggctt taattttgcc accaatccgt tgatgtaaat aaacagctcg
     1381 ccgcaatgct ggttggtcgt cctggttagg atcgataaca acgagcatgt tctgatacat
     1441 agccatacag ggtctcctta caacaactgt caacgcagtt tgtaattaaa agattaaccc
     1501 atatctggtg aatgaaacag tgatgaacct tctgccagat caataaatca gaaaaattta
     1561 atgatatgac agaaggatag tgagttatgc ggaaaaatca ggcaacgtta cgcgtatggc
     1621 cagcaagctg ggccagcgca tcgttatttt cgatggtgat gtatttacct ttgactgcca
     1681 gcataccgct tttctggaag cgacccagca gacggctgat ggtttctacc gtcaggccca
     1741 gatagttacc aatatcgcca cgagtcatcg tcaggcggaa ttcacgaggg gagaagccgc
     1801 gttgggcaaa acgacgggac aggttgtaga tgaatgcagc cagacgttcc tcggcatttt
     1861 tcttcgacaa cagcaagatc atgtcctgat cgcctttgat ttcaccgctc atcagacgca
     1921 tcatctgctg acgcagattc ggcattttac cggacaaatc gtccagcgtt tcgaacggaa
     1981 tttcacatac catcgaggtt tccagcgcct gagcgaagct cgggtgatgg ccgctgccga
     2041 tggcgtcaaa tcccactagg tcgcctgcta aatggaaacc agtgatttgc tcgtcgcctt
     2101 gctcagtgat tgtataactt ttaatcgtac cggagcggat ggcataaagc gatttaagtt
     2161 catcaccagc cttaaacagc gtctggcctt tctgaatagg cttcttccgc tcaatgatat
     2221 tatcaagctg atcaagctca tgttcgttga gtgtgaacgg gatgcaaagc tggctgatgc
     2281 tgcaatcctg gcaatggata gcacaaccgc cagactgaat gcgccgtata attcgctttt
     2341 ccgggatcat aggtctgctc aagccgtaat tgatatttgt caattttaac atctttttag
     2401 ggagcaagta agtctaagca aaccttaaca gcagagaatt ccgatattag atgtaaatat
     2461 atgtctatct atttgaaact ccttaagttg tcaagggtaa ctttacataa aagtgtgaac
     2521 aagctggcac aaattgttta atgtttacag caaaagataa ccttcgtggc gcaataacca
     2581 ctcttttcgc tgaactccgc ctgcatatcc ggtcatggtg ccgtttcggc caataacccg
     2641 atggcaaggt acgacgatgc tgatgggatt cgatccgttt gccgcaccaa cggcacgcgc
     2701 cgcgccaggg cggcccaatt gttcagccag ttggccgtaa tgcattacct gcccgcaggg
     2761 gatagtgcgt agtgttttcc agacttcgcg ctgaaatggc gtcccccccg tggcagtggg
     2821 aagcgtatca ataatgctaa gattaccggc aaaatattca cgaagcttgt cgcttaaacc
     2881 acctgggttg gtggcagaaa tgcgctcata gccttctttg cgataatgga tgtccagcag
     2941 ctgcaccatg cgttcgctgt actcttccca ttcaaccgcc cgcaggcgaa attgctcatc
     3001 gcaaatcacc cacagtggac ccagtggcgt ggcaattttt tcttcaagta atctcagcat
     3061 ccgttctctc ttaagacaaa cgtgggtaaa taccctgacc tatcggcaga ccgacaagat
     3121 accacgccag caacatcagc agccatacca ccaaaaagat aagtggatag ggcaagacta
     3181 acgaatagta agtacccagt ttcgcgtctg gtttgtcgca gtctgatagc ataaagcgat
     3241 tttttaattt ttctgcctga agaaatgagg gcaagccatc acatggatcg agggtgggat
     3301 aattccagtc agtgaaattt taccgtaggc tatctgttat ttgattctgt ggcgtgatag
     3361 ccgccagaca ccactgaact cgtcactcat tccccaatcc cggcgagaat gtggttattt
     3421 acagagctaa aaaatgaata aaaaaataga gcctgtcgac atccgccaga ctctacagta
     3481 cacacagcag tgcatcctcg tcttaaatcc ggcctggggt cctggtgacc ggtgacagtg
     3541 ttataacggt tttacccatc cgttgccaat tttcttcagt gacagatttc acgaaaatat
     3601 aagttaaatc atttgtcttg tgattgcata attatgcatc gttaatcgac gagtaaattt
     3661 accgggcgag tagcccgttc gacgcttaaa catattgccg aacgattcag ctgaggcata
     3721 gcccagacga tgggcaattt tggtcaacga atatccatca tcaagaagcg tcactgcaga
     3781 aataacgtgc atttgctgaa cccagtggcg aaatgttaat cccgtctctt taacaaataa
     3841 gcgcatgagt gtacggctac tgacgttaat tttatccgct agattatcca gtgttggcag
     3901 atacccttgg ttgtttagca aattctcaca aagctgctgt agccgctcat catttggcca
     3961 agggagttca agtgacattt tcacccccat ttttatttcc tgaagtatca gtgtctcgag
     4021 ggctgaaaaa cgtaactgtt gtttatctgt ggtactttgc gacggtggaa gttggttaat
     4081 tgcaattagt aattccctta acaggggtga aatacccgtc atgaagcaaa ctgttcccag
     4141 cgtttcactt tccgcttctg aaaccagtac ggtttttaaa agtacgttgc ctgtcgcaac
     4201 aaacttatgc ggcatttgtg ggggcagcca aagcgcgctg ctggcatgga ctagccagac
     4261 gctttctcca gcccatacac gaacgactcc ttcagccggg taaagcaact gggcatagag
     4321 atgtttatgc cagggaactg tacctccatt cagatgttcc gaatccagtc cgataatgtg
     4381 atgccctggt gatgctttgg gttgcagtgt aattgtcatt tacagtaggt attttgtcag
     4441 aaatcatcaa ataagccatg ttaaatttta aatatgacgt atgcaagttt ccggatatat
     4501 acggtgtaaa gagtgatgat aatttctaaa aaacagctta ttggggtggt agcgatcggt
     4561 attcttttga ctggcgttgt cttttttatc tggagggtaa gcaaaggaag atttatacag
     4621 acgacggatg atgcttacat tggtggtaat atcaccacgg tggcaagtaa agtttctggt
     4681 tatatttctg ctatagaggt gcgcgataat cagagcgtaa aaaaaggcga tattatcctg
     4741 cgtctcgatg atcgggatta tcgtgcaaat gtcgccaggc ttgaggcaaa aataaaaagt
     4801 agcaaagcca atctcgaagg cattcaagcc actattacga tgcagcagtc gataattcaa
     4861 agtgccagtg aaacatggca ggcagtaaaa catgaggagc aaaagcgcct gcgagatact
     4921 gaacgttatg aaaaactcgc acaatcagcg gcaatatcgc aacaaattat tgataacgcc
     4981 aggtttgact accagcaggt cgcagcaaag gagcgcaaag ccgcgaacga tttcctggtg
     5041 gagaagcaac ggctggcagt tttatctgct caggaagaaa acgtacgtgc gagtatcgaa
     5101 gaagttcagg ccgctttgac tcaggcacta ctggatctgg aatataccc
//
LOCUS       DAZXUK010000251         5114 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZXUK010000251 DAZXUK010000000
VERSION     DAZXUK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.251"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(337..582)
                     /locus_tag="ACH3L8_003514"
     CDS             complement(337..582)
                     /locus_tag="ACH3L8_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086469.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1359..2201)
                     /locus_tag="ACH3L8_003515"
     CDS             complement(1359..2201)
                     /locus_tag="ACH3L8_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFT4086470.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2286..2483)
                     /locus_tag="ACH3L8_003516"
     CDS             complement(2286..2483)
                     /locus_tag="ACH3L8_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFT4086471.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2503..2991)
                     /locus_tag="ACH3L8_003517"
     CDS             complement(2503..2991)
                     /locus_tag="ACH3L8_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFT4086472.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2988..3365)
                     /locus_tag="ACH3L8_003518"
     CDS             complement(2988..3365)
                     /locus_tag="ACH3L8_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFT4086473.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3412..3789)
                     /gene="cbeA"
                     /locus_tag="ACH3L8_003519"
     CDS             complement(3412..3789)
                     /gene="cbeA"
                     /locus_tag="ACH3L8_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFT4086474.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3869..4090)
                     /locus_tag="ACH3L8_003520"
     CDS             complement(3869..4090)
                     /locus_tag="ACH3L8_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFT4086475.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4177..4653)
                     /gene="radC"
                     /locus_tag="ACH3L8_003521"
     CDS             complement(4177..4653)
                     /gene="radC"
                     /locus_tag="ACH3L8_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFT4086476.1"
                     /translation="MQQLSFLPGEMTTRERSFILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(4669..>5114)
                     /locus_tag="ACH3L8_003522"
     CDS             complement(4669..>5114)
                     /locus_tag="ACH3L8_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFT4086477.1"
                     /translation="SACAPETDLQQLVATLVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT         1193 a         1243 c         1355 g         1323 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgttgg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctgggtggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gccggactcg gaatcgaaaa tttcttttat
     1261 catattgata ttaatgcaat ttgtttggtt tgattaaaaa tgtaccctca attatacccg
     1321 cagtcccttt tgatatcgaa agggaatcac tctcgggatc aggctgagag cacagaagga
     1381 aagtacctgg ctattatatc gttaattctg tcaatcagtt ccagcctttt gaaagtgatg
     1441 tgtgcagatc ccttcttaaa gtatttaatg atgaacatct cgtcttcata gcgctcttta
     1501 ccctgaacgg actggatgtg accatccaga cgaatagtga tgttttcccg gttgtcgggg
     1561 atcggtttgc cgctgaacag atgtagcatt ctttccaggt cgacgagtcg gtcagtctgt
     1621 tgcccggtga taagatgaaa cccccaccgg tcccacctca ccaggttgtt gacgataatt
     1681 ttactgccaa atttacaggg tgagttggtt ttgtaatccc agctcagccc tttgaagacg
     1741 ttgatcactc cccgttcaaa cacttcatct ttgttctggt gcagttgttc aaatgtgctc
     1801 agaatgttcg cttcgctgat ttccggaaaa ttatcatatt ccagtgacct gtaccatgtg
     1861 tcccgggtct gcgcatccat cagactcagc atcccggaac gttgcatcag atcacgccag
     1921 agttcgcgat caagattgcg tgtgatggct ttcattgcgg tttccggttt ctccatcagc
     1981 cagcaaccgc agcgaaaatc ctgtttcatg gcccagtcca gagccgtttt acctctaata
     2041 ctttctgtca ttgctgatac ttcagccagt tgtcggataa gtgtgtcgat ttgcagcaat
     2101 gtactctttc gtacagccag aatgtggcgg atactggttg agcagataac gtccgtatgc
     2161 ccgttgacca cttccggacg agaggctgtg atttcagaca taatcgatat tcctttttgc
     2221 agataaacga aaacgccagc cgtattcagg ctggcgtcag tggaatgaag tctggtgagt
     2281 aagactcacc ggttctgttg cagaaactgc agctgacgca gggtacggat atcttccctg
     2341 gcctgttcaa tacagggcag cagtgctgcc gaatccgttt tatcctcatc gttgtcgaag
     2401 ataatccctg attcgcagtc aatattgtcc tgcagccacg caatcagaat atccagcgcc
     2461 gtttctgtgg ttaataattt catccagtgt cttttgtcct gattaccagt cgaaagtggg
     2521 gaaccccggc agacattctg cgccggcgtc cagatgaatg agactgacgc cataacgccg
     2581 ggtgagagtg acgaccagac gacggaacgt tctggacagg cccaggcgtt taagccgcag
     2641 aaccgggtac gaccatgcat cggtacgtaa cagatagccg ctgcctgtaa aatgaatcca
     2701 ttctgtttcg ccgtcgtcat tagtctggtg tgacagcgag tacagccagg cgttgtccgt
     2761 ttccgtgata tgtgcggtgc tgcaacgtat gcaggtaagg gttgttaacg gtggtggtgt
     2821 actggttgcg tgttggtcgg atttatcgac cacgcggagt gaataaccgt tttcagagac
     2881 cttgttaatc agttcactga gattaacacc atcgacgtca acgacaatgc gccccatgtt
     2941 cagtgcctgc acgttaacgc tgtcggcttc cagcgtcagg gccagtttca ttgtttcgct
     3001 tccggatgct tcccccgggt aatgttattt accgttctgt agttatcgcg ggtcatcagg
     3061 ccggttgccc ggcgtgcccg gagaatatca atgctgttga ttaactgcga cggggctcct
     3121 gcgctgaatc ccggctggtc ggtacgtacc agcgcgtatt tttcaacgag aaagttcacc
     3181 gcgtcacaca gtgaaattcc tgcctcaata tgctgctcaa tcacacgttc atcagcgaac
     3241 ggtgtgtcat tcagcgtgag gccatagtgc tggtccagca gtcgggtgag cagtgtctgc
     3301 cagatggtga tgggagacgg gcagcccgat gcctcccgta cgtgagtgtc gggtaatgta
     3361 ttcatcttaa gttctctgta acaggtggtt ataaagggga gatatccggt gttatacggt
     3421 ggttgcgggc gctgccggtg tcggataaac agccagataa acgtaaccac aggagccgag
     3481 ggtgtcggct tcgcaggtca gccctttcgc atacagtgtg acggtatgct gatggcgggg
     3541 attcagttca ccgccagtga gcatgagttc cagttgtttc atcagcagcg gaaaggcctg
     3601 gtccaggtgg tacgcatcta cgtcgctgaa cctgcctctg ataccggcgc ggtctgcaag
     3661 gtaatgcagg cggttaccct cctgtaccag tcgggccccg aaacagggcg tcacggtgca
     3721 gggcagcccc caccaggggc ggtcgtgatt gtcgtcggga ggcgttgtcc cggggagtgt
     3781 gtctgacacg ataaagtcct cacagaaaat cggttaagaa tggtccggta gtggcgataa
     3841 ttctgctcat cagaattccc ttccagactc agttcagggt gacgctcatc agccggacat
     3901 acggaccaaa actgtcctta cggcgttctg caaacacagc cagtacaccg ggaatatcct
     3961 gcacttcacg gccggtatat gtatccgtgc taccgtgcca gcggtattta ccggtacaga
     4021 acggaaaaag acgggatgca ggatgctgac ggtgaatacg catggcttca ccacgggtga
     4081 tgattttcat gagaatagtc ctcctgaatg tgaaagtaag aaacagctga gaaagagaaa
     4141 ctgaagtgat atgaaggtga ttgtgacggt gacgggttaa agcagaccgt gttccgcaaa
     4201 ggagaaaacc tggctgccac cgactatcag atggtccggt acccggatat caaccagggc
     4261 cagtgcctgt accagacgct cagtgataag gcggtctgcc ttgctggggg ggacttcacc
     4321 ggacgggtga ttgtgcgcca gcaccacggc agcggcattg tggtacaggg cgcgtttaat
     4381 cacttcccgg ggatggactt ccgtacggtt gatggtgccg gtgaagaggg tttcaccggc
     4441 aatcagctga ttctggttgt tcagatacag cacccggaac tcttcacgct ccagtcccgc
     4501 catgttcaga atcagccatt cccgtgccgc atgcgtggag gtgaaggcca cgccgggttc
     4561 atgaagatgg cggtccaggg ttttcagggc ccgcagaatg aagctgcgct ctctggttgt
     4621 catttctccg ggcagaaagg aaagctgctg cattgtccct gccaccattc agtcgatgat
     4681 gcgcataatg gcgctgcatt ccggatgctg cagggcgtaa tcccgcagcc ggtaataatg
     4741 gaccgtcatg gcataacact ccgtacgaca ggcatgatga ctgtacgtca tcagacaggc
     4801 agcaatgccg gcagcttccg gactcatttc agcgcggtta ccgttcatgg cattgaacag
     4861 tacccatgtt tcgtcatcat cgtcatccgg ttcgggtgcc ataaatgccc cgccgttgtt
     4921 cagggtgtac agattccaga taccaccgca gtagtcttcg cacagacggt ccatccagcc
     4981 gaagacacgg ggctccaggg tcacccactg tggaatgagg ccaaagtgct gcggccagaa
     5041 gctgatgcgc tgttcatcag ggacgagggt ggcaaccagt tgttgcaggt cagtctccgg
     5101 tgcacaggcc gaag
//
LOCUS       DAZXUK010000252         5045 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZXUK010000252 DAZXUK010000000
VERSION     DAZXUK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.252"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(11..322)
                     /locus_tag="ACH3L8_003523"
     CDS             complement(11..322)
                     /locus_tag="ACH3L8_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFT4086478.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="ACH3L8_003524"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACH3L8_003524"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="ACH3L8_003525"
     CDS             701..1054
                     /locus_tag="ACH3L8_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFT4086479.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACH3L8_003526"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACH3L8_003526"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFT4086480.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="ACH3L8_003527"
     CDS             complement(2810..3280)
                     /locus_tag="ACH3L8_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFT4086481.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACH3L8_003528"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACH3L8_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /locus_tag="ACH3L8_003529"
     CDS             complement(4119..4337)
                     /locus_tag="ACH3L8_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFT4086482.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACH3L8_003530"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACH3L8_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFT4086483.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         1352 a         1170 c         1213 g         1310 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gagga
//
LOCUS       DAZXUK010000253         5038 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZXUK010000253 DAZXUK010000000
VERSION     DAZXUK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.253"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..213)
                     /locus_tag="ACH3L8_003531"
     CDS             complement(<1..213)
                     /locus_tag="ACH3L8_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFT4086484.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="ACH3L8_003532"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="ACH3L8_003532"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFT4086485.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="ACH3L8_003533"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="ACH3L8_003533"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFT4086486.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="ACH3L8_003534"
     CDS             2894..3634
                     /locus_tag="ACH3L8_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFT4086487.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="ACH3L8_003535"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="ACH3L8_003535"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFT4086488.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="ACH3L8_003536"
     CDS             complement(4192..4794)
                     /locus_tag="ACH3L8_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFT4086489.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4864..4929
                     /gene="yobI"
                     /locus_tag="ACH3L8_003537"
     CDS             4864..4929
                     /gene="yobI"
                     /locus_tag="ACH3L8_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFT4086490.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
BASE COUNT         1281 a         1221 c         1341 g         1195 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctgg tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc
     4861 gcaatgtata tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt
     4921 ccaatataac ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg
     4981 caagttttct tcatccttcg ctggatatct atccagcatt tttttatcat acagcatt
//
LOCUS       DAZXUK010000254         5024 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZXUK010000254 DAZXUK010000000
VERSION     DAZXUK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.254"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="ACH3L8_003538"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="ACH3L8_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFT4086491.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="ACH3L8_003539"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="ACH3L8_003539"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFT4086492.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="ACH3L8_003540"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="ACH3L8_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFT4086493.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="ACH3L8_003541"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="ACH3L8_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFT4086494.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5024
                     /gene="yidZ"
                     /locus_tag="ACH3L8_003542"
     CDS             4378..>5024
                     /gene="yidZ"
                     /locus_tag="ACH3L8_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFT4086495.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1208 c         1256 g         1416 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cctg
//
LOCUS       DAZXUK010000255         5014 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZXUK010000255 DAZXUK010000000
VERSION     DAZXUK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.255"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..322
                     /locus_tag="ACH3L8_003543"
     CDS             <1..322
                     /locus_tag="ACH3L8_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4086496.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="ACH3L8_003544"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="ACH3L8_003544"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFT4086497.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="ACH3L8_003545"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="ACH3L8_003545"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFT4086498.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACH3L8_003546"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACH3L8_003546"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFT4086499.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="ACH3L8_003547"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="ACH3L8_003547"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFT4086500.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="ACH3L8_003548"
     CDS             complement(3726..4955)
                     /locus_tag="ACH3L8_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFT4086501.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
BASE COUNT         1284 a         1286 c         1218 g         1226 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcg
//
LOCUS       DAZXUK010000256         4974 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZXUK010000256 DAZXUK010000000
VERSION     DAZXUK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.256"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACH3L8_003549"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACH3L8_003549"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFT4086502.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="ACH3L8_003550"
     CDS             1020..1703
                     /locus_tag="ACH3L8_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFT4086503.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="ACH3L8_003551"
     CDS             1700..2161
                     /locus_tag="ACH3L8_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFT4086504.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="ACH3L8_003552"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="ACH3L8_003552"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFT4086505.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="ACH3L8_003553"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="ACH3L8_003553"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFT4086506.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACH3L8_003554"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACH3L8_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFT4086507.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACH3L8_003555"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACH3L8_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFT4086508.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(4903..>4974)
                     /locus_tag="ACH3L8_003556"
     CDS             complement(4903..>4974)
                     /locus_tag="ACH3L8_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086509.1"
                     /translation="RNYSEYSEYTSVFFLSLMIFIKS"
BASE COUNT         1176 a         1305 c         1240 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tacg
//
LOCUS       DAZXUK010000257         4952 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZXUK010000257 DAZXUK010000000
VERSION     DAZXUK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.257"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(103..891)
                     /gene="phoH"
                     /locus_tag="ACH3L8_003557"
     CDS             complement(103..891)
                     /gene="phoH"
                     /locus_tag="ACH3L8_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFT4086510.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1498..2763)
                     /gene="efeB"
                     /locus_tag="ACH3L8_003558"
     CDS             complement(1498..2763)
                     /gene="efeB"
                     /locus_tag="ACH3L8_003558"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFT4086511.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2769..3896)
                     /gene="efeO"
                     /locus_tag="ACH3L8_003559"
     CDS             complement(2769..3896)
                     /gene="efeO"
                     /locus_tag="ACH3L8_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFT4086512.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3954..4628)
                     /gene="efeU"
                     /locus_tag="ACH3L8_003560"
                     /pseudo
     CDS             complement(3954..4628)
                     /gene="efeU"
                     /locus_tag="ACH3L8_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
     gene            4717..>4952
                     /locus_tag="ACH3L8_003561"
     CDS             4717..>4952
                     /locus_tag="ACH3L8_003561"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086513.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEG"
BASE COUNT         1168 a         1371 c         1199 g         1214 t
ORIGIN      
        1 tccatattac gtgggtagga tcagtttcag aaaaagcgct agtccgggca taaacaccca
       61 aaacaaagcc cggttcgccc gggctctgca ccgataatac acttaactgt atgcatgcag
      121 cgtacgttgg caaagtgccg aacgtacgca atcctctttt ccgaaccgga cgatcccaac
      181 catttcatct tcttcgaaac gttccagcgc gtcacttaat ccggagcaca cgccgcgagg
      241 caaatcgcat tgcgtgatat caccgttgac gataaccgtc acgttctccc cgaggcgggt
      301 taaaaacatt ttcatttgcg cggcagtcac attctgcgcc tcgtcaagaa tgacaactgc
      361 attttcaaag gtacgtccac gcatataggc gaacggcgcg gtttccacct tccctatttc
      421 cggtcgcagg cagtactgca taaaggaagc ccctaagcgc cggaccagca agtcgtagac
      481 cgggcgaaaa tagggagcaa acttttctgc gatatcacca ggtaagaagc caagatcttc
      541 atcggcttgc agaactggac gggtgacgat aatcctgtcg acatccttat gtatcagggc
      601 ctctgccgct tttgctgcgc tgatccaggt ttttccgcac ccggcttcgc ccgtggcgaa
      661 tatcagctgc ttactctcaa tagcctgcag atagtgcaat tgcgcttcat ttcgcgcaag
      721 gatgggcgag gtatcgcgac tgtcgcgggc cataccaatg gcttctacgc cgctcatctg
      781 cacaagcgag gtgaccgatt cttcttcacg ctgcttatgg ctgcgcgaat cccgtctcag
      841 cacacgtttt gcctcgcgac gagctttgat cactgctttt tgtcttccca tggagagcac
      901 cttgagttgt ttgtattcat cacacgcgcc gttggcagcg cgattatgcg cacgaacatc
      961 agagggttgg cttccttgta agccatagtt tgcttttgga taaaatgccg aaaaacggct
     1021 acgcacaccg tttgcggcgt cggtaacacg agtaaagaaa ggaagtgaga aaaatacaga
     1081 gtgacgaagt tgctaccgga ggagaaatct ccgcgagtgg tggagcgttg cttaacaaaa
     1141 cattgtccag aacaggacgt taccatccgc gatctccata gtgactgact atcactgccg
     1201 ggaacttccg ctgctactta ataagtacaa cagaactcgc attcattgca acaatatatt
     1261 cacttatatt taacgataac acccatttcg gtgacattaa tttctactgg aaagatgaca
     1321 gagtgatgac agtgatggaa aaagctgtgt gctttccgca ggattcgcag ggctggtaaa
     1381 atgtgcctcg tgtttgccgg attcggcgcg aacgccttac ccgacctaca agtgattaat
     1441 aatttgtgca caaaactcat gcccgataaa cctgcgcatc gggcatattt gcgtcgttca
     1501 aacctgcaat agcgcccttc ccagataatc gttcgcgtcc ttcactcccg gcagcgaaaa
     1561 aaaataaccg ccgccgatag gtttaacgta ttcctccagc gcttcgccat tgagcctttt
     1621 ttgtactgtc aggaagcctt tttccagatc gtgttggtag cagacaaaca gcaaccccat
     1681 atccagttgt ccggagttgg tgacccccaa cgaataactg tagccgcgac gcagcatcag
     1741 gctggactca ctctccgccg tgcggggatt cgccagccgg atatggctgt ccagcgcgat
     1801 caccttccct tccgggtcgc tggcgtaatc aggcacatca tgctcatgct gcattcccag
     1861 cggcgcaccg gtttgcttat cacggccaaa aatcgtctgc tgttctttca gcggcgttct
     1921 gtcccaaaat tccactcgaa actgaatcaa gcgtactgcc tgatagctgc caccgattgt
     1981 ccacgcaggc tcctgctgat ctgccgttac ccacaccact ttttgcatca acttatcatt
     2041 ctggctatcg ggattggcag tgccgtcttt gaaacccagc aaattaatcg gcgtctcttt
     2101 gcctttacta cgcgccgcgt gatcggaaat aaacccttcc cgcttccagc gcacactgag
     2161 caaatccggc gtgtgtttga tgatatcgcg cagcgcatgg ttcaccgtgt cctgggtgtt
     2221 ggcgcaaatc tgtagcaaca catcaccatg acataacgcc gcatccagcg agtcgttggg
     2281 gaaacgcgtc atcttctgca gcttttttgg catctgtggc gcaaggccaa agcgctcatc
     2341 aaacaatgag tgacccaccg ataacgtgat ggtgagatta tcgggcgcaa tgtagccgcc
     2401 aagaatgccg gaatcgagtg gtggcaggcg cggatttggc gtttctggtg ctgctccgcc
     2461 ctgactcaga aaagcaaaac gctgagtcaa caagcgaaac aaccgctcaa gatcggcttt
     2521 atcgctggca agcacatcaa acgccaccag catcattgcg gcctgttgtg gcgtcaggat
     2581 ccctgcctga tgctcaccat aaaacggctg tttctcattg cgtgcatccg gtgaaagcgt
     2641 gcccggtgca ctttgcgttt tttgtgcatg agcgaccgga caacttcccg ccagcgcacc
     2701 aatccctttc agtaaacgtc ggcgtgacgg ttcattcacg ccgttttcat ctttatactg
     2761 cataacgctt aatccagtcc cagcacaccg cgaagttgcg ccagatcttc cgccagcgca
     2821 gtaatcggcc ctttcagcgc attccggtcg gcatcggtca atttgtcgta ggtttcaaaa
     2881 ccgtctttag tacggtattt cgccagaatg gtatcgacct ttttaaagtt ggcatcgact
     2941 tttgccaaca gttccgggtt ggctttttgc agttgtggac gcagcagatc gacaattttc
     3001 tgcgagcctt caacgttagc ctggaaatcc cacagatcgg tgtggctgta gcgatcttct
     3061 tcaccgctga ttttgctggc tgccacttcc tcaatcagtc cggctgcgcc gccgaccact
     3121 tttgaaggtg ggaaagccag ttcactgatg cgtttttgca aatcgaccac atcggtataa
     3181 agctgctcag cgtactgatc catccctttg gtggtgttgt cgccaaacaa tgctttttcc
     3241 agacggtgga aaccagtgaa ttttgggtcg gcggcttttt gctcgtaatc atcttcacgg
     3301 gcgtcaatgc tgccatccag atcggagaac agttcagcaa tcggttcaat acgctcatag
     3361 tgctggcgcg tcggcgcata cagtgctttc gctttttcga tatcgcctgc tttaatcgcg
     3421 tcggtaaagg ctttggtgtc ggtcaccagc tgcgtggttt ccgccatgac atacgcttta
     3481 tatgcagtaa ttgcaccacc aagacttaac agcgcatcac tttgcgccgc atccgccgtt
     3541 gcctcacctt tgacgatcaa cttccctttc gggttagtca gcagaccgca ggtcatatcg
     3601 tattcgccag gctgtaaatt cgccgtcatt ttctggctaa aaccaggggc gatattttcc
     3661 cgctcttcca ccaccatcac gcctttgagg atctcccact ccagcgcctt ctggctatgg
     3721 ttctgaataa tgaactgtgt tttcccggcg ttaaccgtaa tggtcatcgg ttcgcactgc
     3781 ttatccgtca cggtcacttt gacctgcggc acatcagcgg cgttagccat aaaagcagaa
     3841 gaaaacagcg cagccacgct caactgcaat gcgttacggc ggaagttaat ggtcatgacg
     3901 agactatccc tttaaagtat gttgtaacta agcaagagtt tgcgtcgtat ttgttacgcg
     3961 gagcgagacg ctgtcgcccc tgcgcgtggt ggcagagcaa atgccaccag cgccgggatg
     4021 agataaataa accagacggc gacttcgctg acgctcggtg cttcctgata gccaaaaatc
     4081 ccttccatca gcgtgccaaa cagcgagtga gttgagagga ccgcgctcat atcgaaggcg
     4141 atttcctgaa agtggttcca caatccggct tcatgaaatg cgcgaatggc accagccgcc
     4201 agccctgcgg cgacgaagag aataaacagg ctggtccatt taaaaaatgc accaagattg
     4261 aggcgaatac cgccccagta gagcaggaag ccgagcacca cggcagtagc aagaccgagc
     4321 attgcaccca gcggcggcca gatcccgaca tcttgttgaa atgccgccag caggaaaaag
     4381 accgactcca gcccttccct tgcaacggca aaaaagacca tcatcaccag cgcccagcca
     4441 tgatgatttc cacgctgcaa tgcgctatcg actgcctgtt ccagttgcac tttgacgttg
     4501 cgcgacactt tgcgcatcca gaaaaccatc caggtaagga tcaccacggc gatcaccgcc
     4561 acgatacctt caaacagttc ctgctctttt tgcggaaatt cgccggtggt ttcgttaatg
     4621 aagatccccg agtaggcagc ctgacggctg cggcttgtca tggtctgcaa ttaccgttat
     4681 aaaaacaggc aatgtcattg tctttcaggt ggttatatgg cccgttcagc taaaccccgt
     4741 aaacgtaaac ccgcctctca aagaagcaaa cttccccgct atgtcgtgaa gcttcacgaa
     4801 gatgattttt ttgacgaaga agacgcagaa gttctgcgct ttgatagttt tgacgatgcc
     4861 gttgaatgct gcgcagacct gaatattccg ttctttgtgg atgcgggaaa caaaaagctg
     4921 gtcttctggt ttgtacgtgt tgatgacgaa gg
//
LOCUS       DAZXUK010000258         4935 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZXUK010000258 DAZXUK010000000
VERSION     DAZXUK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.258"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..374)
                     /locus_tag="ACH3L8_003562"
     CDS             complement(<1..374)
                     /locus_tag="ACH3L8_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid family protein"
                     /protein_id="HFT4086514.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQK"
     gene            complement(386..875)
                     /locus_tag="ACH3L8_003563"
                     /pseudo
     CDS             complement(386..875)
                     /locus_tag="ACH3L8_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(879..2099)
                     /locus_tag="ACH3L8_003564"
     CDS             complement(879..2099)
                     /locus_tag="ACH3L8_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFT4086515.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2103..2846)
                     /locus_tag="ACH3L8_003565"
     CDS             complement(2103..2846)
                     /locus_tag="ACH3L8_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFT4086516.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(2737..4203)
                     /locus_tag="ACH3L8_003566"
     CDS             complement(2737..4203)
                     /locus_tag="ACH3L8_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFT4086517.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(4203..4935)
                     /locus_tag="ACH3L8_003567"
                     /pseudo
     CDS             complement(4203..4935)
                     /locus_tag="ACH3L8_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
BASE COUNT         1043 a         1402 c         1227 g         1263 t
ORIGIN      
        1 tacttctgcg tatcaatcgg gcggccgacc tgctgagcag cctgcaattc tatgaccgtc
       61 cagccaagtt ccatccccca caggttcagc gggttaccgg atttgccgat atccacgttc
      121 acgccagcaa tagcggttga gtctttgcct acccagtttt ttccattcgg atttgcgcca
      181 gtacccgcag cggcgaagct ggtattcgtc cagctggaaa tgtcatctgc gatggaggcg
      241 tcttcacgca gttgaatatc gcggctccag gtgtacccca ccagtggcag gttcagcgtc
      301 tggtcgagtc tctccagctc cccgatgaga aaggcaccag agctgtcaac ggttgcctga
      361 tcaaaagtaa tcattcgtct gttccttaaa tcttccagga aatttctgca ttgccgtcag
      421 catcaccggc acctgtgaat tcagcgttgg tcagcaccac gtttttgcca ctgactgacg
      481 tggacatgaa tccacccaga ggcactttga tggattcatc agtggagatc gccttttttg
      541 atagtgctgg catcaaaatc agaaccgaga ttaacggtca cgtagccacg cttcatggcg
      601 tcgcccggga agttcttgcc tgttcccacc tggcgaacca tgtccggctg cgacgtggtc
      661 ggataagggc gcacgtagat ccccttcacc ttgtctgcgg tatcaccatc tgccagcggc
      721 acgaaaaaac cgtcatcatc gtatttacca gccaggccat aggcagcgaa ggcgttatcg
      781 gatttaagga ccaccggttc gacggttaag tcctgcgggc gagagacagc cccggcaatg
      841 ccaacaggca tccggtacag aaatacatta ttcatttttt accctttacg gtttgcccag
      901 aattcagcgt tttgtttgtt cagggaagcg atactggtca tgcccatgtt taggcgctgt
      961 gcatcgccgg tggtggcgcg ggtgtttcgc cctttggcaa cctcagacac ggcattaaac
     1021 gccatgtcga ccgattgttt cggtaatttg cggatatccg catcaccgac tatctggcga
     1081 accagcgttt tgtcagcaga agccagaacc tcgcgtttga acgcggtcgg tttcatctta
     1141 cggctcagat cgatacccgg gacgataact tcggcacgcc aggctgagtc accagtaatc
     1201 gtggtttcct cttcatcgtc ctcgccgtca ccggtcggat tatcgtcagg cttattatcg
     1261 ttatcgcccg tggcatttct ttccagctta gccagcaggg ctttcagtaa tgttttgagg
     1321 tcatcatcac tgtcgccggt tggatctccg cccatctctg gtgctttgtc cggtagtggt
     1381 tgctgcgggg acaggttgat gttgagatta acgccctgcg gcaaatcccc ctcatctcct
     1441 gtaaccgatg cgggagccga ctccaccagt tcgttcatgg tgtcggcatc tcctgtcttg
     1501 atggccgcac gcatgcggtt ccaccagttt ttcttttgat ttgccattgt gtctctgtct
     1561 ccaattgcac aacgatttcc ggctctgcct ttagggacaa gagccacatg gtttccggta
     1621 atatcgacct gctcggcttt acctggctcg gtctgctcgt actccgcgtc atagccgcac
     1681 gacacttcgc gcaggccatc ttcgataagc tgaatggcgc tttcgtcttt gacgataagg
     1741 tcagccagca tcaaatcaga ctgctcaccc gtcccgcgcc ggacattctg gaggtgcccg
     1801 acagcaagca ctttccagtt ctcgggattt accagccgca cattcccgtt ttcatcttca
     1861 ggatgcagga tcgtgatgct catcccttcg aatgaggcaa gcgtggccgg atggaatacc
     1921 tgctcaggag aacgcgtgac gactatttca acgaacttat cgggtttcag ttttggcagg
     1981 tcatcagcac catagagctg cttacctgtt cgtcctatcg gcacgtcttt gcacagcaac
     2041 gagccgtcag ccaggcggta tcgggtttcc ccgagcctgg tctcaaaaaa gtatttcatg
     2101 ggttatgctg cccttttcat tgcgaatgga acgcgaacaa acacgatctc tttatagcag
     2161 cggcagtttg gcagctcgcc cgcgtggccg gtcatgccgt ccaaagttgg aggtgaatcc
     2221 cagcggacga atttaccttt catgtgatcg tgggaatctc ggacatcgcc atcatcggct
     2281 gtacgccaaa tataaccatc cgagccaata gctatcgctc gggccatatc cagcgcgccc
     2341 gttgctcttc ccagttccgt tcgggcaatc agatcggccc tagacttagc aacatcgccg
     2401 gtagatgcaa tctctttagc aaactgctcc gcccgaccac cggtgacaac agcctctatc
     2461 gctttgttct ggatgtcgta caccctgtct gcggcctcga gaggcagaga tttgatgtac
     2521 ttgacctgtt cggcgatgat ggattgcatc acctggccca caggagcgct ttccacaaga
     2581 ttgcggagct cgcgactgat gttcttgctg tgttgccgcc aaactttctc gttctgccgg
     2641 gttaggtccg cagtaaagtt ttccgcgacc tttgtcgccc agggggtgat gatttcactg
     2701 taacgctcca gcgccgcaat aatttccgtg atactgtcat ttgaaccatc gtagcgacca
     2761 tttacgatgt ctccgaccgc cctcgctatc ctgcgtaggc tggttcgata ccggatttcc
     2821 gcctgacggc tcctgcggtt cgtcatcagg ttcgccgatg ccgggcggcg cttcatcttc
     2881 ggcattctcg atgtcctcgt tggtaatgga tgcccctatg ccggttacgt cagaattttc
     2941 tcgcaaatca gtcatagcgg ctttcagtgt catcagacca tcacccagcg ccgtactgat
     3001 tgcgttggtg gtgtttaacg ccaccgttga gcgatcgaca tcagacattt gccagagcgg
     3061 gttaaactca aacgtgaaat cgtccggcag cggcttgcca agttccgaac gatgcatgat
     3121 gtccagtatc cgccgcaccg gaagacgtaa acgcctctcc tgcaacgaac tgatgcgatc
     3181 gtaatagttg gcaaggtctg catcaccggt agaaaatcct ttcggggact gtccgaacaa
     3241 ccgcaccagt gggataccaa cagcgccact aatctgttct gcaaactgcg aaaggatgtc
     3301 atccagacca ctgaagctgt actgatgggt ttcaaactta tcccgcgagt ccatgagcgt
     3361 catgccttca ttactctgga actgtcgaat caggtcgata ttcttcagca acgcttcata
     3421 cgcaggacca ccaagtgcga taagctcgcg tagcttctcc acgctgtagg tacgcaaatg
     3481 cgccttgtag accagctgcg ccgcgccgac agtagcgctg tcgaacgcgg taagacgatc
     3541 ccagatacgc tctacaaccg acattcccca ttcgttctcg gtcatcttct gctgaaatgg
     3601 caacgtgacg ccatcaaagc gaatcaggcg actgtgatga atgcgccagg caggaattcc
     3661 cgttgctgtg gtcaccacat cgtaaaactc aggtttaccc aggtccggcc ccatatcttt
     3721 aatgcggcgg gtcagtgccg ggtcgaccat ccagcggtcg agcgggagaa tccccttaaa
     3781 cttgccctta ccgatggttt cgggtcgcag cggggtcatt ggtgcctgcc cctcaatcat
     3841 gatgaaaccc accgcgccgc cgtagaggcg cgaccatttc agcacgtcat tcagcgcatc
     3901 ccagatttgc aactcatcca gttgtgattc gagaatgcca cgatcttttg catcaatttc
     3961 cgaagtgatg cgaatgcctt tgcgggtcat atcatccggg atagcatcga ctgcttcgcc
     4021 gatgatccag gatgaacgat aggaccattc caccagcatg cggttacgac tggtgaaatt
     4081 agcccggtag gtggatgctg agtgctggtt aggtgtctgc atccctactc gggcaataaa
     4141 attctcataa ccatcagctg tggcctgcac agttcgccgc agggcttgtt tgtttcgtgc
     4201 catcaggcct gtctccccag ctgttcccag atatccagcg atgtatcgat tggcgcgaag
     4261 gccataatga atgcatcagc aacgtttggt gacggtatct cgcgttttgc gaggtctttt
     4321 ttactttcga ccatcacacg tccgttacgg tcgaaatcac gatgaggtgt tgtcagttcc
     4381 agtttcagct tttcaagcaa cggacaacga gaatctatgc tgatcagctc atccacagga
     4441 tactgttctc cgttgttaat ggcgttaaac gtatttctga aacggtcagc caccatgcct
     4501 gagccttaag atttgcgaaa aagtctttgt tggggatgcc gttgtactcg tcatctggct
     4561 catgcacacc agcaccggcg ttaaacctct ggtaattcac acgtcgcgcg tatgcgttct
     4621 cgctcttccg gtcagcgtta atttcagaga atttagcacc ggcagacgca ccaacaccga
     4681 tagagtcgta aacaatatct gcttcacgct ccagcgccgc ctgataagta cgctggcagc
     4741 tcttcagtaa ttcatcttct ttggccttcc attcgtcggc ccagaaaaca acggatccgt
     4801 gacggtaaac gttagcgcac ttatctgtac cactgtcagc cacgtcaaag ccaatacgct
     4861 ttcttccact gggttcgaaa tttaacgttt tgtgcgcatc cactgcggct tctatccaag
     4921 acagtttgat gattg
//
LOCUS       DAZXUK010000259         4914 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZXUK010000259 DAZXUK010000000
VERSION     DAZXUK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.259"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            223..882
                     /locus_tag="ACH3L8_003568"
     CDS             223..882
                     /locus_tag="ACH3L8_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFT4086518.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1083..1460)
                     /locus_tag="ACH3L8_003569"
     CDS             complement(1083..1460)
                     /locus_tag="ACH3L8_003569"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFT4086519.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1527..4493)
                     /locus_tag="ACH3L8_003570"
     CDS             complement(1527..4493)
                     /locus_tag="ACH3L8_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFT4086520.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4496..>4914)
                     /locus_tag="ACH3L8_003571"
     CDS             complement(4496..>4914)
                     /locus_tag="ACH3L8_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFT4086521.1"
                     /translation="ISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKE
                     HLSFTGEDSPMANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSER
                     IAELRQRVEAGEQKTKLAREFGISRETLYQYLRTDQ"
BASE COUNT          985 a         1337 c         1487 g         1105 t
ORIGIN      
        1 gacgcacttt gcgccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat
       61 cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt
      121 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
      181 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      241 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      301 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      361 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      421 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      481 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      541 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      601 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
      661 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
      721 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
      781 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
      841 gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat
      901 tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc
      961 agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg
     1021 cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct
     1081 tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata
     1141 tgatcattta ttctgcctcc cagagcctga taaaaacggt tagcgccggg gttggatttt
     1201 tcagcgttcc agctaaggct aaggcattcc tgttcaagcg caagccgggc tataaagcgc
     1261 attatcgctt tacccgtgcc cttatttcga tcgcactgag aaacatacag ctctttaata
     1321 tgcagctgac cgctgtatcg gggcgaggga taaagaatat tgcaacatgc caggccagta
     1381 atgttattgc cgcagcgcgc tctgatcacc agggtaccgg aaagccggtt gaataacttt
     1441 ttacagagat aatccttcat caacgcttcc tgaatgatac cttcgccata ataggggtcg
     1501 tctcagaaaa cggaaaataa agcacgctaa gccggttgca gaggccgtag cggcctgaac
     1561 ttccccgcgc cgatcttggc gctgctgcgc cataggtaat caccggtcag gttgatgtgc
     1621 tcccagccga gtggcgacag gtactgcaat agcgagtcat cgacggcatg accattgccg
     1681 cgcaacgcat gcgccgcacg ctccaggtag accgtgttcc acagcacgat ggccgccgtc
     1741 accaggttga ggccgctggc ccggtagcgc tgctgctcga aactgcggtc acggatttca
     1801 ccaaggcggt tgaagaacac ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc
     1861 ccggcatgca cgcggcggcg tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg
     1921 cgctcgatgc ggcccaactc gcgcagcgcg acggccaagc cgttctggcg cgggtagctg
     1981 ccgagtttcc tgagcatcag cgaggccgtc accgtgccct gcttgatcga ggtggccagc
     2041 cgcaggattt cgtcccaatg ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc
     2101 ggcttgagcg cgtcataggc ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg
     2161 tcgcggatgc gcggcgcgaa gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg
     2221 gtgaagcccg ccgtgtcggt gtagtgctcc tcgatccgca ggtcggattc gtggtacagc
     2281 aggccgtcga gcacgtaggt tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat
     2341 ggcgcgtatt ggtcggagat gtgggtgtag aaagtccgtc ctgggctgct gccatatttt
     2401 gggttgatgt gccccgtgct ctttgccttg ctagcggttc ggaaattctg tccgtccgat
     2461 gatgatgtgg tgccatcgcc ccagtgcccg gcaaagggat gccgaaactg agcgttgacc
     2521 agttcagcca acgctgtcga gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg
     2581 agcttcgcgt aggtcgtgcc ggggcaggac tcggccatct tggtcaggcc caggttgatc
     2641 gcgtcggcca ggatcgtggt caacaacagg ttcttgtcct tggccagatc gcccgatttc
     2701 aagtgcgtga agtgccgggt gaagcccgtc cactcatcga cttcgagcag cagttcggtg
     2761 atcttgacgt gcggcaggac catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc
     2821 accgccgcat ccagcggcgt gatcttcaag cccgactcgg tgatgatggc atccggcagg
     2881 tcgttggctg ccgccatgcg gttgacggtg gcaagttgtg cttccagcag cgtcagccgc
     2941 tcatgcagat attgttcgca gtcggtggcc acggccagcg gcaattcgct ggactgcttg
     3001 aggctggtga acttctcggg cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa
     3061 ccctgcaccc agatgtcgcc cgagcgcagg gagttcttca actcggacag cgcgcacagt
     3121 tcgtagtagc gccggtcgat gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg
     3181 atgaagccgg tcggtgcatc ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc
     3241 agcacctcaa tggcatcaag cacgtttttg gcggcgggcg cggcccgcag cttgagcacg
     3301 gcaaggaatt ccggtgcata gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg
     3361 aaatcgaagt catcgggttg cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa
     3421 tcccaggaca tgacggcctc gatggcggca aacgcatcgc ggcctgattg cttggcgtcg
     3481 atcagcgcct gaccgatgcg cccgtacaga cgtaccttgg cgttgatggc cttgcctgac
     3541 gcctggaact gctgctgatg cttattcttg gcagcgttaa acagcttacc caggatgcgg
     3601 tcgtgcaggt cgatgatttc gtcggtgacg gtggccatgc cctcggtggc cagcgccacg
     3661 agagtggcgt agcgccgttg cggctcgaat ttggccaggt cggcgggtgt catctggccg
     3721 ccctcgcggg caatcttgag caggcggttc tggtgaacca gccgctcgat gccggtaggc
     3781 agatcgagtg cctgccatgc cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt
     3841 ggcttggccg gagactggcg caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc
     3901 ttgagcagat cgtcgaggcg gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg
     3961 tagatgcgcc ggttagcacg ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg
     4021 gcgggcagaa tgaccgactg ccgccgcagg tgcccgatca aggcgctggc cagcacgatg
     4081 cctttgtcgg tttgcatcgc cagctcggtc agcatctgga cggcctgccg gtaatggctc
     4141 atggtgaagg gccggaaacc gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc
     4201 tgctcccgct ggccgtactc gttccagctt tcgacgccga ccttgagctg gtcggcgacc
     4261 agcttcaaca agggcgggaa cggtagttca tcgacgccca ggatgacgcc gggaaagcgc
     4321 aggtaacaga gctgcaccgc gaagcccagc cgattggctg gcccgcgccg ctgtcggatg
     4381 atcgagaggt cggtatcgtt gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc
     4441 aacgccagca ggctttcccg ctcggcggcg gacaggatgg aacgacgtgg catatttact
     4501 gatccgttct caagtattga tacagggttt cgcgactgat tccgaattca cgagcaagct
     4561 tggtcttttg ctcgccagcc tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca
     4621 gggatttctt cctgccacgg taagccccgc gttgcttggc gagcgcaata ccctcgcgct
     4681 gacgctcgcg gatcagggcg cgctcgaact cggcgaacgc gcccatcacc gagagcatca
     4741 ggttcgccat cggagagtct tcgccagtaa aactgaggtg ttccttgacg aattcgatat
     4801 gcacgccgcg ttgtgtcagc gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca
     4861 ggcgatccat gctatgcacc accacggtgt cgccggtgcg ggcgaagctt atca
//
LOCUS       DAZXUK010000260         4870 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZXUK010000260 DAZXUK010000000
VERSION     DAZXUK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.260"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..527
                     /gene="ydcF"
                     /locus_tag="ACH3L8_003572"
     CDS             <1..527
                     /gene="ydcF"
                     /locus_tag="ACH3L8_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFT4086522.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="ACH3L8_003573"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="ACH3L8_003573"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFT4086523.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACH3L8_003574"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACH3L8_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="ACH3L8_003575"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="ACH3L8_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFT4086524.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="ACH3L8_003576"
     CDS             4169..4342
                     /locus_tag="ACH3L8_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086525.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4368..4436
                     /gene="yncP"
                     /locus_tag="ACH3L8_003577"
     CDS             4368..4436
                     /gene="yncP"
                     /locus_tag="ACH3L8_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFT4086526.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACH3L8_003578"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACH3L8_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFT4086527.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..4804)
                     /locus_tag="ACH3L8_003579"
                     /pseudo
     CDS             complement(4664..4804)
                     /locus_tag="ACH3L8_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1120 c         1208 g         1295 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat tccgtaacag catccttcag ttcgcttata ttactgaact
     4801 cattagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
     4861 aatactagtt
//
LOCUS       DAZXUK010000261         4808 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZXUK010000261 DAZXUK010000000
VERSION     DAZXUK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.261"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..321
                     /locus_tag="ACH3L8_003580"
     CDS             <1..321
                     /locus_tag="ACH3L8_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFT4086528.1"
                     /translation="FAMTIDECATWIAQTGDSESWRQWENGKCAIPDCVVEQLLAMRQ
                     QRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIYQSVAAELYAHDL
                     ERLC"
     gene            421..1626
                     /locus_tag="ACH3L8_003581"
     CDS             421..1626
                     /locus_tag="ACH3L8_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4086529.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            1809..3080
                     /locus_tag="ACH3L8_003582"
     CDS             1809..3080
                     /locus_tag="ACH3L8_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4086530.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLSEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            3092..3958
                     /gene="ydiB"
                     /locus_tag="ACH3L8_003583"
     CDS             3092..3958
                     /gene="ydiB"
                     /locus_tag="ACH3L8_003583"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFT4086531.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            3989..4747
                     /gene="aroD"
                     /locus_tag="ACH3L8_003584"
     CDS             3989..4747
                     /gene="aroD"
                     /locus_tag="ACH3L8_003584"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFT4086532.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
BASE COUNT         1207 a         1084 c         1119 g         1398 t
ORIGIN      
        1 tttgccatga ctattgatga atgcgcgacc tggattgccc agaccggtga cagtgaaagc
       61 tggcgtcagt gggaaaatgg caaatgcgcc attcctgatt gtgtagtcga gcaactgttg
      121 gctatgcgtc agcaacgaaa aaaacatctt catgccatca tagagaaaat aaataaccgc
      181 atcggcaaca acaccatgcg ttttttcccc gacttaaccg cgtttcaaca agtctaccct
      241 gatggcaatt ttattgactg gaagatctat caatcggtag ccgctgaatt atatgcacac
      301 gatctggagc ggctttgtta atttttccac agaaaggaat tgtcgttgtt acaacaataa
      361 tgaatggata ctgacacaac atcgctacac tttttaaagc acctttgcta agtagaacct
      421 atgaaaaatc cctattaccc taccgcactt gggttgtatt ttaattacct ggtgcatggt
      481 atgggcgtca ttttgatgag cctgaatatg gcctcgctgg agacactttg gcagactaat
      541 gccgcgggtg tctcgatagt tatctcatcg ctgggcattg gtcgattaag tgtcttgctt
      601 tttgcaggat tattatccga tcgctttggt cgccgccctt ttatcatgct cgggatgtgc
      661 tgctatatgg ccttcttttt tgacatcctg cagaccaata acatcattat cgcttatgtt
      721 tttggctttc tggcgggaat ggcaaacagt tttctcgatg caggcactta tcccagtttg
      781 atggaagctt ttccacgctc acctgggaca gccaatattt taattaaagc atttgtttcc
      841 agcggacaat ttttattacc gctaatcatt agcctgttag tgtgggctga actgtggttc
      901 ggttggtcct ttatgattgc tgcaggcatt atgtttatta acgctctgtt tttataccgt
      961 tgtacgttcc caccccatcc gggtcgtcgc ttacctgtca taaagaaaac caccagctct
     1021 acggaacatc gctgttcaat tatcgattta gccagttata ccttatatgg ctatatctca
     1081 atggcaacgt tttatctggt tagccagtgg ctggcacagt acggacaatt tgttgcaggc
     1141 atgtcataca ctatgtcgat caaactactc agtatctaca ccgtgggttc gctgctttgt
     1201 gtatttatta ccgctccact cattcgtaat accgttcgcc caacaacatt actgatgctg
     1261 tacaccttta tctcatttat tgctctgttt accgtctgcc tgcatcccac attttatgtg
     1321 gtgataatat ttgcttttgt cattggtttt acctctgctg gaggtgttgt gcaaattggc
     1381 ctgacgttaa tggctgaacg tttcccttac gctaaaggta aagctacagg gatctattac
     1441 agtacgggca gtattgcgac ctttactatt ccgttgatta cggctcatct gtcccaaaga
     1501 agtattgccg atattatgtg gttcgatact gccatcggtt ttttactggc actgtttatc
     1561 ggcttacgca gccgcaaaaa aacgcggcat cactcgctaa aggaaaatgt cgctccgggt
     1621 gggtaatgca atattctttt caggtcatgc aagatcttac ggataaataa ctctttctgc
     1681 gctaactaag gaaaatcgcg atcaaaaaca aactatgaca tgcaatattc ctggaaacat
     1741 aaactttatg ccatgtaccc agggaaaatc atcttcagta tagtaattat gtaaaccgtc
     1801 ggagaacaat acgtacggta acgaaattat ctttcagcac gccatttatc ctggctgttc
     1861 tttgtattta cttcagctat ttcctgcacg gcattagtgt tattacgctt gcccaaaata
     1921 tgacatctct gtcagaaaag ttttccactg acaacgcggg cattgcctat ttaatttccg
     1981 gtatcggttt ggggcgattg atcagtattt tattcttcgg tgtgatctcc gataagtttg
     2041 gtcgtcgggc ggtgatatta atggcagtaa taatgtatct gctattcttc tttggtattc
     2101 ccgcttgccc gaatttaact ctcgcctacg gtctggcagt gtgcgtaggt atcgctaact
     2161 cagcgctgga tacgggtggc taccccgcgc tcatggaatg ctttccgaaa gcctctggtt
     2221 cggcggtcat actggttaaa gcgatggtgt catttgggca aatgttctac ccaatgctgg
     2281 tgagctatat gttgctcaat aatatctggt acggctatgg gctgattatt ccgggtattc
     2341 tgtttgtact gatcacgctg atgctgttga aaagcaaatt ccccagccag ttggtggacg
     2401 ccagcgtagc taatgaatta ccgcaaatga acagcaaacc tttagtctgg ctggaaggtg
     2461 tttcatcggt actgttcggt gtagccgcat tctcgacctt ttatgtgatt gtggtgtgga
     2521 tgcccaaata tgcgatggct tttgctggta tgtcagaagc tgaggcatta aaaaccatct
     2581 cttattacag tatgggctcg ttggtctgtg tctttatttt tgccgcacta ctgaaaaaaa
     2641 tggtccggcc catctgggct aatgtattta actctgcgct ggcaacaata acagctgcca
     2701 ttatctacct gtacccttct ccactggtgt gtaatgccgg cgcctttgtt atcggtttct
     2761 cagcagctgg cggcatttta cagctcggtg tttcggtcat gtcagagttt ttccccaaaa
     2821 gcaaagccaa agtcaccagt atttatatga tgatgggtgg actggctaac tttgttattc
     2881 cgctgattac cggttatctg tcgaacatcg gcctgcaata tatcattgtt ctcgatttta
     2941 ctttcgcgct gctggccctg attaccgcaa ttattgtttt tagccgctat taccgcgttt
     3001 ttattattcc tgagaatgat gtgcggtttg gcgaacgcaa atttagcacc cggttaaaca
     3061 caattaagca tagaggttaa aggagttaat tatggatgtt accgcaaaat acgaattgat
     3121 tggattgatg gcttatccta tccgccacag tttatcgcct gaaatgcaga ataaagcctt
     3181 agaaaaagcg ggattgccat ttacctatat ggccttcgaa gtggataacg atagctttcc
     3241 cggagcaatt gaaggattaa aagccctcaa aatgcgcgga actggtgtgt cgatgccgaa
     3301 caaacaactg gcgtgtgaat atgttgatga attaacgcca gcagccaaac tggtgggtgc
     3361 catcaacacc atcgttaatg atgatggcta tctgcgtggc tataacaccg acggcacggg
     3421 ccatattcgc gccattaaag agagcggttt tgatatcaaa ggcaaaacga tggtgctgtt
     3481 aggggccgga ggtgcctcaa cggcgattgg cgcgcagggg gcaattgaag gtttaaaaga
     3541 aattaaactc tttaaccgtc gggatgagtt cttcgataaa gccctcgcct tcgcgcagcg
     3601 ggttaacgaa aacaccgatt gtgtcgtcac ggataccgat ctcgccgatc agcaagcctt
     3661 tgctgaagcc ctggcttccg ccgacatttt aaccaatggc acaaaagtag gtatgaaacc
     3721 ccttgagaat gaatcattgg ttaatgatat cagtctgtta catccgggac tgctggtcac
     3781 tgaatgcgtg tataacccgc atatgacgaa gttattgcag caggcacaac aagctggttg
     3841 caaaacgatt gatggatacg gcatgttgtt gtggcaaggg gctgaacagt tcacgttatg
     3901 gactggcaaa gatttccctc tggaatatgt taaacaggtc atggggttcg gtgcctgaca
     3961 ggctgaccgc gtgcagaaag ggtaaaaaat gaaaaccgta actgtaaaag atctcgtcat
     4021 tggtgcgggc gcacctaaaa tcatcgtctc gctgatggcg aaagatatcg cccgcgtgaa
     4081 atccgaagct ctcgcctatc gtgaagcgga ctttgatatt ctggaatggc gtgtggacca
     4141 ctttgccgac ctctccaatg tggagtctgt catggcggcg gcaaaaattc tccgtgaaac
     4201 catgccagaa aaaccgctgc tgtttacctt ccgcagtgcc aaagaaggcg gcgagcaggc
     4261 gatttccacc gaggcttata ttgctctcaa tcgtgcagcc atcgacagcg gcctggttga
     4321 tatgatcgat ctggagttat ttaccggcga tgatcaggtc aaagaaaccg tcgcctacgc
     4381 ccacgcgcat gatgtgaaag ttgtcatgtc caaccatgac ttccataaaa cgccggaagc
     4441 cgaagaaatc attgcccgtc tgcgcaaaat gcagtccttc gacgccgata ttcctaagat
     4501 tgcgctgatg ccgcaaagta ccagcgatgt gctgacgttg cttgccgcga ccctggagat
     4561 gcaggagcag tatgccgatc gtccaatcat cacgatgtcg atggcaaaaa ctggcgtaat
     4621 ttctcgtctg gttggtgaag tatttggctc ggcggcaact tttggtgcgg taaaaaaagc
     4681 ctctgcgcca gggcaaatct cggtaaatga tttgcgcacg gtattaacta ttttacatca
     4741 ggcataagca ataatatttc ggcggaaata ccctcccccg ccgaaataaa aaaagtattt
     4801 ttaaccgt
//
LOCUS       DAZXUK010000262         4735 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZXUK010000262 DAZXUK010000000
VERSION     DAZXUK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.262"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1179
                     /gene="proV"
                     /locus_tag="ACH3L8_003585"
     CDS             <1..1179
                     /gene="proV"
                     /locus_tag="ACH3L8_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFT4086533.1"
                     /translation="NLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGVKDASLAIE
                     EGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAM
                     VFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGG
                     MRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLD
                     EAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTP
                     NGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAA
                     LIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG
                     "
     gene            1172..2236
                     /gene="proW"
                     /locus_tag="ACH3L8_003586"
     CDS             1172..2236
                     /gene="proW"
                     /locus_tag="ACH3L8_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFT4086534.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2294..3286
                     /gene="proX"
                     /locus_tag="ACH3L8_003587"
     CDS             2294..3286
                     /gene="proX"
                     /locus_tag="ACH3L8_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFT4086535.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3478..4657
                     /locus_tag="ACH3L8_003588"
                     /pseudo
     CDS             3478..4657
                     /locus_tag="ACH3L8_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1053 a         1245 c         1270 g         1167 t
ORIGIN      
        1 aatctttata aaatatttgg tgagcatcca cagcgagcgt tcaaatatat cgaacaagga
       61 ctttcaaaag aacaaattct ggaaaaaact gggctatcgc ttggcgtaaa agacgccagt
      121 ctggccattg aagaaggcga gatatttgtc atcatgggat tatccggctc gggtaaatcc
      181 acaatggtac gccttctcaa tcgcctgatt gaacccaccc gcgggcaagt gctgattgat
      241 ggtgtggata ttgccaaaat atccgacgcc gaactccgtg aggtgcgcag aaaaaagatt
      301 gcgatggtct tccagtcctt tgccttaatg ccgcatatga ccgtgctgga caatactgcg
      361 ttcggtatgg aattggccgg aattaatgcc gaagaacgcc gggaaaaagc ccttgatgca
      421 ctgcgtcagg tcgggctgga aaattatgcc cacagctacc cggatgaact ctctggcggg
      481 atgcgtcaac gtgtgggatt agcccgcgcg ttagcgatta atccggatat attattaatg
      541 gacgaagcct tctcggcgct cgatccatta attcgcaccg agatgcagga tgagctggta
      601 aaattacagg cgaaacatca gcgcaccatt gtctttattt cccacgatct tgatgaagcc
      661 atgcgtattg gcgaccgaat tgccattatg caaaatggtg aagtggtaca ggtcggcaca
      721 ccggatgaaa ttctcaataa tccggcgaat gattatgtcc gtaccttctt ccgtggcgtt
      781 gatattagtc aggtgttcag tgcgaaagat attgcccgcc ggacaccgaa tggcttaatt
      841 cgtaaaactc ctggcttcgg cccacgttcg gcactgaaat tattgcagga tgaagatcgc
      901 gaatatggct acgttatcga acgcggtaat aagtttgtcg gcgcagtctc catcgattcg
      961 cttaaaaccg cgttaacgca gcagcaaggt cttgatgcgg cgctgattga tgcgccgtta
     1021 gcagtcgatg cacaaacgcc tcttagcgag ttgctctctc atgtcggaca ggcaccctgt
     1081 gcggtgcccg tggtcgacga ggaccaacag tatgtcggca tcatttcgaa aggaatgctg
     1141 ctgcgcgctt tagatcgtga gggggtaaat aatggctgat caaaataatc cgtgggatac
     1201 cacgccagcg gcggacagtg ccgcgcaatc cgcagacgcc tggggtacac cgacgactgc
     1261 accggctgac ggcggtggtg ctgactggct gaccagtacg cctgcgccaa acgtcgagca
     1321 ttttaatatt ctcgatccgt tccataaaac gctgatcccg ctcgacagtt gggtcactga
     1381 agggatcgac tgggttgtta cccatttccg tcccgtcttc cagggcgtgc gcgttccggt
     1441 tgattatatc ctcaacggtt tccagcaatt gctgctgggt atgccagcgc cagtggcgat
     1501 tatcgttttc gctctcatca cctggcagat ttccggggtc ggaatgggcg tggcgacgct
     1561 ggtctcgctg attgccatcg gcgcaatcgg tgcctggtcg caggcaatgg tgactctggc
     1621 gctggtgtta actgccctgc tgttctgtat cgtcatcggt ttgccgttgg ggatatggct
     1681 ggcgagaagt ccgcgagcgg cgaaaattat tcgtccactg cttgatgcca tgcagaccac
     1741 gccagcgttt gtttatctgg tgccaatcgt catgctattt ggtatcggta acgtgccggg
     1801 cgtggtggtg acgatcatct ttgcgctgcc gccgattatc cgtctgacca ttctggggat
     1861 taaccaggtt ccggcggatc tgattgaagc ctcgcgctca ttcggtgcca gcccgcgcca
     1921 gatgctgttc aaagttcagt taccgctggc gatgccgacc attatggcgg gcgttaacca
     1981 gacgctgatg ctggcccttt ctatggtggt cattgcctcg atgattgccg tcggcgggct
     2041 gggtcagatg gtacttcgcg gtatcggtcg tctggatatg gggcttgcca ccgttggcgg
     2101 cgtcgggatt gtgatcctcg ccattatcct cgatcgcctg acgcaggccg ttgggcgcga
     2161 ctcacgcagt cgcggcaacc gtcgctggta caccactggc cctgttggtc tgctgacccg
     2221 cccattcatt aagtaactct gcacttgccc ggtgacgccg ggcattatca ccctgccaaa
     2281 aaaaggaata acaatgcgac atagcgtact ttttgcgaca gcgtttgcca cgcttatctc
     2341 tacacaaact tttgctgccg atctgccggg caaaggcatt actgttaatc cagttcagag
     2401 caccatcact gaagaaacct tccagacgct gctggtcagt cgtgcgctgg agaaattagg
     2461 ttataccgtc aacaaaccca gcgaagtgga ttacaacgtt ggctacacct cgcttgcttc
     2521 cggcgatgca accttcaccg ccgtgaactg gacgccactc catgacaaca tgtacgaagc
     2581 tgccggtggc gataagaaat tttatcgtga aggggtattt gttaacggcg cagcacaggg
     2641 ttacctgatc gataagaaaa ccgccgacca atacaaaatc accaacatcg cacaactgaa
     2701 agatccgaag atcgccaaac tgttcgatac caacggcgac ggtaaagcgg atttaaccgg
     2761 ttgtaaccct ggctggggct gcgaaggtgc gatcaaccac cagcttgccg cgtatgaact
     2821 gaccaacacc gtgacgcata atcaggggaa ctacgcagcg atgatggccg acaccatcag
     2881 tcgctacaaa gagggcaaac cggtgtttta ttacacctgg acgccgtact gggtgagtaa
     2941 tgagctgaag ccagggaaag atgtggtctg gttgcaggtg ccgttctccg cactgccggg
     3001 cgataaaaat gccgatacca aactgccgaa tggcgcgaat tacggcttcc cggtcagcac
     3061 catgcatatc gttgccaaca aagcctggac cgagaaaaat ccggcggcag ccaaactgtt
     3121 tgccattatg cagttgccag tggccgatat taacgcccag aacgccatta tgcatgacgg
     3181 caaagcctca gaaggcgata ttcagggcca cgttgatggc tggatcaaag cccaccagca
     3241 gcagttcgat ggctgggtga atgaggcgct ggcagcgcag aagtaatttt tattcgggcg
     3301 gataaggcgt tttcgcctca tccgccgctc tgtacacaat gcctgatgcg acgctgacgc
     3361 gtcctatcat gcctacaaac cacatcgcac atttcaacaa gctatctttc atcccatatt
     3421 catcaacatc cgctattatt gatttccagc ttaatcatca cctgatgaac aaaaataatg
     3481 actaaaccta atcatgagct tagcccggcg ctgatcgtgc tgatgtctat cgccaccggt
     3541 ctggcggtag ccagtaacta ttacgcccag ccattgctcg acaccatcgc gcgtaacttt
     3601 tccctttccg ccagttcggc aggctttatt gttaccgccg cgcagttggg ctatgccgca
     3661 ggtctactgt ttcttgttcc cctcggtgat atgtttgaac gccgccgcct gattgtctcg
     3721 atgaccttac tggcagcggg cggtatgtta atcactgcca gcagtcagtc actggcgatg
     3781 atgatcctcg gtacggcatt aaccggttta ttctcagtcg tggcacaaat tctggttccg
     3841 ctggcagcga cgctggcttc accggataaa cgcggcaaag tggttggcac cattatgagc
     3901 ggtctgctgt tggggatatt gctggcacga accgttgccg ggttactggc aaacctcggc
     3961 ggctggcgca ccgtcttttg ggttgcttcg gtgttaatgg cactgatggc gctggcatta
     4021 tggcgtggtc tgccacaaat gaaatcagaa acccacctca actacccaca gttgttgggt
     4081 tccgttttca gcatgttcat cagcgataag atcctgcgca cccgcgcgtt gctgggctgc
     4141 atgacctttg ccaacttcag cattctctgg acctcaatgg cctttttgct tgccgctcca
     4201 ccttttaact acagcgatgg tgacattggt ctgtttgggc ttgcgggagc tgccggagcg
     4261 ttgggcgctc gtccggcggg cggttttgcc gacaagggca aatcgcacca caactttcgg
     4321 tctgcttctg ctgttacttt catggctggc aatctggttt ggtcacactt ccgtactggc
     4381 gttgattatc ggcattctgg tgctggacct caccgtacag ggcgtgcata tcactaacca
     4441 gacggtaatt tatcgaatac atcctgatgc gcgtaatcgc ctgaccgcag gttacatgac
     4501 cagctacttt attggcggtg ccgccggttc gctaatttca gcctcagcct ggcaacatgg
     4561 cggttgggct ggcgtttgtc tggctggcgc gacgattgcc ctggttaact tactggtctg
     4621 gtggcgaggt tttcatcgtc aggaagccgc aaattaagca atcagcaacc gttttcgtgg
     4681 ctttacactt ataagggtgt taagaagccc atcagtctga taaggttaag atatt
//
LOCUS       DAZXUK010000263         4702 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZXUK010000263 DAZXUK010000000
VERSION     DAZXUK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.263"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="ACH3L8_003589"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="ACH3L8_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFT4086536.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="ACH3L8_003590"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="ACH3L8_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HFT4086537.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="ACH3L8_003591"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="ACH3L8_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFT4086538.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACH3L8_003592"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACH3L8_003592"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFT4086539.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACH3L8_003593"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACH3L8_003593"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFT4086540.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..>4702
                     /locus_tag="ACH3L8_003594"
     CDS             4449..>4702
                     /locus_tag="ACH3L8_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFT4086541.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGL"
BASE COUNT         1273 a         1211 c         1071 g         1147 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acaccccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ct
//
LOCUS       DAZXUK010000264         4677 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZXUK010000264 DAZXUK010000000
VERSION     DAZXUK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.264"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..321)
                     /locus_tag="ACH3L8_003595"
                     /pseudo
     CDS             complement(1..321)
                     /locus_tag="ACH3L8_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            401..1594
                     /locus_tag="ACH3L8_003596"
     CDS             401..1594
                     /locus_tag="ACH3L8_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HFT4086542.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1620..2297)
                     /gene="istA"
                     /locus_tag="ACH3L8_003597"
                     /pseudo
     CDS             complement(1620..2297)
                     /gene="istA"
                     /locus_tag="ACH3L8_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
     gene            2644..3453
                     /locus_tag="ACH3L8_003598"
                     /pseudo
     CDS             2644..3453
                     /locus_tag="ACH3L8_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3650..4429
                     /gene="ipaJ"
                     /locus_tag="ACH3L8_003599"
     CDS             3650..4429
                     /gene="ipaJ"
                     /locus_tag="ACH3L8_003599"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFT4086543.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
BASE COUNT         1315 a          906 c         1172 g         1284 t
ORIGIN      
        1 gagtggatga tgctgggggg ggttgtcgaa gctcaccaga ttttcaccag gatgcacgtc
       61 atactctttt ttctccggag gcagtgccag catggactgc tgctcttcga gccagcgatc
      121 acagggacgg gcctggattg tttcatgctt tcgttggtta gcgacatcgt gcagccagcg
      181 cagaccgtgg cggttggctg tttcaacatc gacagtgctc cccatcgggc gcaggcgagt
      241 cattagtggg atgtaaaaac tgttacgggt gtactgcacc atccgttcca ccttaccttt
      301 agtctgtgcc ctgaagggct gaggaatccc cagaaaacga gacaaacata gcattatgtg
      361 atctactgat ttgtgccaac aattcaatga gatcactgct atgcctgttc ataaagtatg
      421 tcagaatttc ttccgggatg ccttagcacc atttcatcag taccgacaaa atgccctgat
      481 ggatgccaca atggcgttaa tcaacggagc atctctgacg cagaccagta tcggacgttt
      541 tttgccgggt aatgcacaag ttaagaataa gatcaaacgt attgatcgcc tcatgggtaa
      601 tgaggcgctc catcgcgata ttccaatgat tttcaggaat atcacttcaa tgctgacacg
      661 gcagttatct ttatgtgtaa tagccgtaga ctggagtggc tatccctccc aggagcatca
      721 tgttcttcgt gccagccttc tgtgtgacgg gcgttccatt ccgttattga gtaaggttgt
      781 tccttctgaa aaacaaaata atccattgat acaacatgat ttccttgatt cccttgctca
      841 atccctgccc cccgatgcca gggttattat tgtcacggat gctggatttc agagcgcatg
      901 gtttcaccat attacttctc tgggctggga ttttattggt cggatcagaa ataacgttca
      961 gtattgcctc gataacgcgc ctgaaagatg gttaaaagtg tcggatagcc ctgaatgtaa
     1021 aacaccggaa tatatggggg ctggaaggct ggttaaagaa aggaaaaaaa gcatccgggg
     1081 gcatttttac acctataaga aatcggcaaa agggcgaaaa aagaaacgtt cgaaaggtca
     1141 gagtggttta aacaaaacag acaaagaaca gagcaaatca gcaaaagaag cctggttgat
     1201 cttcagcagt acaaatgact tcagagcgcg agaaatcatc aagttataca gtcggcgaat
     1261 gcagatagag caaaactttc gtgatgaaaa gaacgggcgc ttcggatttg gtcttcgggc
     1321 cagcaaaagt cgttcaacag gaagaattct ggttctgagt ctgctggcaa cgttgagcac
     1381 tatcgtaatg tggttactcg gttatcacgc tgaaaataaa gggttacacc tgaaatatca
     1441 ggctaacagc attaaatccc ggcgggttat ctcatatctg acgttagcga agaatgttct
     1501 tcgacactcg ccacttattt taagacgaac agtactgagc acagttctaa accatctgtc
     1561 cagaacctac cgaaatatgg tgctggttta ttagcagtaa ttttgtgggg atccctcagc
     1621 ctgaaggggc gacacagtcg gggagagaag cccatctcct tgccgaactg ccacagcgaa
     1681 ggatggaacc ggtgctgacc ggtctgatat gcgtcacgtt gcagaaccac agttttcata
     1741 ttgtcataca acacttcgcg cgacacacca ccaaagaagc ggaacgcatt acgatggcag
     1801 gtctccagcg tgtcataacg catattgtca gtgaattcga tgtacaacat tcggctgtat
     1861 ccgggaacag caacgaacac gtgaagcggt gagcgaccat tacgcatagt gccccagtca
     1921 acctgcatct gtcgtccggg ttcagtttcg aaccgaacgg caggctcctg ctcctgagga
     1981 accgagagag aacgaatgaa tgccctgaga atggtcattc cgccacgata tccctggtct
     2041 ctgatctcgc gagcgattac cgttgccggg attttgtaag gatgagcatc ggcgatgcgt
     2101 tgacgaatat aatcccggta ttcatccagg agtgaagcaa cagcaggtcg cggcgtatat
     2161 tttggcggct cagattttgc ctgcaaataa cgtttaacgg tattgcggga gagccccagt
     2221 tctctggcaa tcgtccggct actcattccc tgcttgtgca ggattttaat ttccataact
     2281 gtctcaaaag tgaccataag ctctcctgaa tcaggagagc agattacccc ctggatctga
     2341 tttcaggcgt tgggtgtgga tcactattgc accgttcgtg acacttgttg actacctgtg
     2401 cggcatctgg tatcaaggtg gcgtgaagcc ggttcaggtt ctcatgcgga acgttggaac
     2461 ctatatcccc ccgataccaa aggcgaaatg taagtgacaa aatgcaatga acacgttctc
     2521 cgttaactga ggagcatggt gataaataaa ggtaaatgca gaaggtgtag agtctcgcag
     2581 atttctgaca tcatagagcc taacattaga tgcataacgc tacagattgt agaatctcta
     2641 caggtgaaat atacactggt tggcgtttat gtcgcaaaac atgtcattca ggttcatctc
     2701 accaataagg atatgagtga agtggaggat aagtgagtct gctgtcagag ttttctggtg
     2761 tatgtcagta actgcgaacc atgtctgctt gatatgggag cttatggagg ctctctgtac
     2821 aaggttaggg agctaacaaa gcagggataa aaagtccgcc tgatgcaggc acggttcgtt
     2881 aaatcttttg ccattggtaa caaaatggtg cgatgaatgt tcaggctatc tttatcttga
     2941 tggtggttca gctgtttggt aaagaaatcg ctgtaacaat agaagaactg caggcagtac
     3001 tggtcccaca tcgtttgctc agtcaactgg tgaagttccg tagcgcgtaa attaatgctt
     3061 tgcatgaaac gttactggag tggatgaaag aatacctgca tatatcatta ctgcgttgga
     3121 ttaccagtac aacagattga atgaactgga catgttgata gacggcattg aaaagcagct
     3181 tataagcata gtgcggcaga gttaattgga gagcacttcg ccttctgagt attaagtaag
     3241 catggtggta cctgcggata ctatctattc actgtgctcg gtcggcagcc ttactgacca
     3301 aggagttggg ggagttgata acaaagttga aaaaacagct ctcaaccaag gtggttattg
     3361 taacaatagc aaataagctg gtgtgacctg tatcacaata accgattatg gacgtaaata
     3421 cgacaaaaat atgtcagtat caggcggtat taattgctga tgctgatgga cattaactct
     3481 taataaaagg gtggatgctg aaaggttgtt atggtgacgg acgtgatgac gacggaaaag
     3541 agtatgaggt tttggattta aagttcgttc ggggaataag gacccggtca gctaattctc
     3601 atagtagttc gcattgctgg atgcaatact gtgctgcaat tactcgtcga tgtcggaaca
     3661 acggaagcct tgcaaacggg gatgtattca tacaggtgtt atgttatatg gggttttatt
     3721 gcagggggct ataccaagag aatatatgat atctcatcag actgatgtga gggtaaatga
     3781 gaatagagta aacgaacaag gttgtttttt ggcgaggaag cagatgtatg acaattcttg
     3841 tggagctgcc agtttactct gtgctgcaaa ggaattaggg gttgataaaa taccacaata
     3901 caaagggtct atgtccgaaa tgacaaggaa gagttctctt gatttggata atcgttgtga
     3961 gcgggattta tacttaataa ctagtggtaa ttataatccg agaatacaca aagataatat
     4021 cgcggatgcc ggttattcta tgcctgataa gatagtcatg gctacgaggc ttcttggatt
     4081 gaatgcttat gtcgttgaag aaagtaacat tttttcgcaa gtaattagtt ttatttatcc
     4141 cgatgcaagg gacttattaa ttggcatggg atgtaatata gtacatcaac gagatgtgtt
     4201 atcttctaac caaagagttt tggaagctgt tgctgtatcc tttattggag ttcctgtcgg
     4261 cctccactgg gtgttgtgta gacctgatgg aagctatatg gatccagctg taggagaaaa
     4321 ttattcttgt ttttcaacta tggaattagg tgcaagaaga agtaattcta actttatagg
     4381 atatactaaa ataggtattt ccatagttat aactaatgag gctttgtaaa ttgtcgtttt
     4441 ttatctgtga aggagcttga atattttggg ggggcataac aaatgatatt actgagtaca
     4501 aatttagcta tattgggcgg attagttgtg tgggaagacg aaatgtttct cttgaaataa
     4561 gtgaactccc tcaaggaatg attgtgtgct taaatattta agtgctggta agtttgagaa
     4621 ggctgacata ttttgcagag caaacagtga ttgacttgga tgatattttt attttag
//
LOCUS       DAZXUK010000265         4676 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZXUK010000265 DAZXUK010000000
VERSION     DAZXUK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.265"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(178..732)
                     /locus_tag="ACH3L8_003600"
                     /pseudo
     CDS             complement(178..732)
                     /locus_tag="ACH3L8_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            875..997
                     /locus_tag="ACH3L8_003601"
                     /pseudo
     CDS             875..997
                     /locus_tag="ACH3L8_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1259..1825)
                     /gene="ipgB2"
                     /locus_tag="ACH3L8_003602"
     CDS             complement(1259..1825)
                     /gene="ipgB2"
                     /locus_tag="ACH3L8_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFT4086544.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2107..2421)
                     /locus_tag="ACH3L8_003603"
     CDS             complement(2107..2421)
                     /locus_tag="ACH3L8_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086545.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(2499..2609)
                     /locus_tag="ACH3L8_003604"
                     /pseudo
     CDS             complement(2499..2609)
                     /locus_tag="ACH3L8_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2933..3610)
                     /gene="ospD1"
                     /locus_tag="ACH3L8_003605"
     CDS             complement(2933..3610)
                     /gene="ospD1"
                     /locus_tag="ACH3L8_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HFT4086546.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(3982..4119)
                     /locus_tag="ACH3L8_003606"
                     /pseudo
     CDS             complement(3982..4119)
                     /locus_tag="ACH3L8_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4201..4499)
                     /locus_tag="ACH3L8_003607"
                     /pseudo
     CDS             complement(4201..4499)
                     /locus_tag="ACH3L8_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1279 a         1022 c          913 g         1462 t
ORIGIN      
        1 agtttttaaa atcaagaaga aaagataagt tttttaaatg cacagtgcct gtggataact
       61 ttgcgtaaat ccggaggaca gatcactctg aagcaagaca acagcatctg aaaacaatat
      121 gctcacgttt caaccacagg gcgtaagtga tcctacccac gtaatatgga cacaggccta
      181 agcgaggttc tggttttcaa attgttccgg actgaggccg ccacaccaac tgtgccgtcg
      241 ccagcgattg taatcacatt cgatataatt aaacactgtt gccctcatta tttcccggct
      301 gataaagtgt tctccgtgga gacattccac tttcagcgaa tgaaagaagc tttccacgca
      361 ggcattatcg tagcagcagc cctttgcgct catactgcca cgcagattat gccacttcag
      421 cagcgcctga taatctgctg aacagtactg acttcctcgg tccgagtgaa cgataacgtt
      481 ccggggtctc ttacgccgcc acagcgccat ctgcagggca tcgcaggcca gttgcgccgt
      541 caggcgtggt gacatggacc agccaataac ggcacgtgac cacaggtcaa tgaccacagc
      601 cagatacagc cagccttcat ctgtacgtaa gtacgtgatg tctcccgccc acttctggtt
      661 cggaccactg gcgtaaaaat cctgctccag cagattttct gacacaggca ggccatgtgc
      721 gcggtaactg accgtattgc tgggtaagat caatttgctc actggtattt actacgtgca
      781 tcgaatgagt gatcaagagc cgtatgacgc gagagtgtca cgtacggttc cgtaagcagc
      841 cggagagtga aactctctcc ggctgactcg actcgcaggt tccgacagcg gtgacgaaca
      901 tgcagcggtg ttgttctcgc tgatcggcac atgccgtctg aacaatgtgg agccagaaaa
      961 atggctgcgt tacgttattg aacatatcca ggactgaccg gcaaaccggg tacgcgatct
     1021 gttgccttgg aaagttgatc tgacttctca gtaaatatca atatgattct gacgagccgc
     1081 ttacctttga gcagagaaag gggagaggga tcccacatcc atgagctagg aaaatacaaa
     1141 aactggctat acaacaattc aatatattag gacttacttg cgaggagaaa tatagcaccg
     1201 ctcaggcaat tcctgggtat ttacttttct tagtttcttt actcgggatg ttctgtcatc
     1261 agaaaggcga ttctaaattt gtaatatagt cactcatatt cccacgtaat ttctttagat
     1321 caatatattg cacatttttc tcaaaaaact gatcggaaat aagatttgca acattgcttg
     1381 ttgtatcatt tttaacgctt ctattcatcc cttcacaaag ttcgtccatt ttctttttga
     1441 aataactgct tcctgataca agatgattta tggaactttg tgcagcattg atgtctagag
     1501 aaatgcgata atctttctca acctgtttaa atatctgttt acggagatta tcgccgattt
     1561 ttcggctgtg ttctgcacaa aacttatcaa tacattggtt taccacccgg gatacgtaga
     1621 cttttttttc atcatttttc caatctgtaa tattttcacc gatttgttct gaaacagagc
     1681 tcaaaatact agtatttatt ttggttgatg atatttttac tatccttttc cccattgtac
     1741 agcggataat ctcatccacc tttcctgaaa aatatctttt gtgagataaa ctgattccaa
     1801 aattattaaa agatgttcca agcattataa tcaccttgtc atgtaaaata gtaacattgt
     1861 cattatttta gctgattcaa acaattctgt aaatgctttg agttctttac tcccttggct
     1921 caatgatgga ccgtctggaa tcccagagac taaactctct gagaaagaga gtatgaaaaa
     1981 acttggggca gtccctaaat gaagggacca acagcggtaa aataaccggc atcattcata
     2041 atgatgccct gttagttgta gttatgaaga tttacgtcat taaaattgct gttcgtagtg
     2101 tcagtcctat ggtctggcat cagctgagga tcgtagccga tacatcactg gcttcacgac
     2161 attcgtgttg aggctattca tgaaaactcc acgctgaaaa cgccgttttg tataaacggt
     2221 cacggtatgc ccggagccac tgttgctgat aaattcgata aaaccttggc gtttcttgaa
     2281 gctatcgtta atgcggataa tgaaacaacg attggtgaga ttcgctcttt tgccgacgcc
     2341 ctggacgctg tccggttcaa tcgcaacaaa atcaaccggc agctgagcaa acccaacctt
     2401 gcctctctgg cgctggagca tgaagtgatt tggctgggac gtagccgctg acagcttcta
     2461 taaaagtatg atcctaccca cgtaatatga acacaggctt aagtgaggtt ctggttttca
     2521 aattgttcag gactgagacc tccacaccaa ctgtgccgtc gtcagcgatt gtaatcgcac
     2581 ttgatataat taaacactgt atatttcatt tgtggagtct ccagtctgag agggctttat
     2641 gcattccgta ttgggcacta tgatgccaga aatctgcaag gctccacacc ttcctgtatt
     2701 tcccttcctc tatcacagtg cactttactt aatagggtgg gatgtgttca ttacatttat
     2761 tgtgtttttt atgattgttt ttgcgtccag tgtcgttcgt catgggaaac tggttctatg
     2821 ggtagtatcg ctcagtctag ttaactgttt gtttcttttt agaaattaag cagatcacac
     2881 aattcgggga gagatccttc taagtgcaat tctttgcagc tatttagcac acttaaaaca
     2941 actcttgtat tttgtcagaa ataatattaa acatgtcttg ttcattattg ttaagtctgt
     3001 ctgctatttc tgtaagtttt atcccactat gatggcatat atttatatat gccatcatag
     3061 cctcgatatt ccctttccct gcggccagaa ataatcctgg taacccctca ttatctctta
     3121 catccaatag tttaatcgtt tcttttggtg ataaatcaaa agttttaata aaatctccat
     3181 aagcttgaat cgcagcggaa tgaccttctt gaagagctat gaacaaagca gattccccag
     3241 cattatttcg tgctgcaaga acttctagtt tttcctcttt gggtatttcg gctgccaaca
     3301 gcgattcacc gtatttacgc gctgcatcgg catttccatg ccaaatcaat gaaaacaaat
     3361 tatggcggtt atactcctta ttattctttg tcggaatgga tatgttctgt attttggcca
     3421 attttctgaa agaaacacca ctatatcccc cccctgattt ttcttcattg atggcgtggg
     3481 atatagcggt atttgtcacg ttaatgatat tatttttcat tcctttattt tcattagcag
     3541 tattgctgcc tattattaag tgcatatttt tgttgtttgc tggatgtaat ccatagttat
     3601 ttattgacat ttttgttcat cctttttgct aaaaaaaaac aataacctat ttttgtaatg
     3661 taatagatat tagtctattg ctaatctaaa aaaaccattg ccacattgaa tggctatatt
     3721 caccaataaa ttctttcgat ttcgtatatt gacatatcgt agatatatct tatccctaag
     3781 attcaataat cgagtccgtt taccattctg tgcaagcgcc ttcactcaga agtgaccgtc
     3841 aaggcgatca ccaaacttga taataaagca gctcattgcc atacgccagt ccttaagggg
     3901 cgttgtccat ttctgtgatg ctgcctagat ttaactgcca gctccggctt atctacatct
     3961 aaatatgtga gcctaatacc gctacacttt gtccagacta tgtttttctc cgggaagtgg
     4021 gctaaccgtc tccataaagt gacgaacttt tttcaacagt ggccatattg aacggcactg
     4081 atgattacgg gttattgtct catgaagagc cggccacatc actgacagca gtgacttcag
     4141 gccttcagca ccagacagcc tgaaccagtt aatccatcgc ccgacaaatt tagtaatcta
     4201 gggctggttt ttcttcagct gttgcggttc gaaagtgcca ttacgatcac gcagagtacg
     4261 gatttccaga ggttcatctc cagtagtgat aatcttcgtt aagcaaccat tgcgggaatt
     4321 gctgccagat ctaagctgat ttttctcgca cccaagatga tgagtcatat tagcattgag
     4381 tacggtttca ttttcagcat ccaatcaaac tgactgagat cttcaggatt tttgagactt
     4441 ttggctagtt cattagctaa agcccgcaac ttattagatg tcaactgttt ttcattcata
     4501 aattcacctt catttgatat caaattaaat agttcaacat cagagaatta tacaaactta
     4561 tgtacaggct cctatgatct tcgaatcctg aagattatag tgaaaataag ttttcacgtt
     4621 gaaatcccac atagttagta tatgataaat aatcatggat gacctgatta acctct
//
LOCUS       DAZXUK010000266         4645 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZXUK010000266 DAZXUK010000000
VERSION     DAZXUK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.266"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            77..1375
                     /gene="exuT"
                     /locus_tag="ACH3L8_003608"
     CDS             77..1375
                     /gene="exuT"
                     /locus_tag="ACH3L8_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFT4086547.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            complement(1463..2551)
                     /locus_tag="ACH3L8_003609"
     CDS             complement(1463..2551)
                     /locus_tag="ACH3L8_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFT4086548.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(2548..>4645)
                     /locus_tag="ACH3L8_003610"
     CDS             complement(2548..>4645)
                     /locus_tag="ACH3L8_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFT4086549.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
BASE COUNT         1102 a         1173 c         1120 g         1250 t
ORIGIN      
        1 cgtaactaac atcttcagcc tctggcggga tgatggccgc gcttcctgcg gatataacaa
       61 aacgatgagg ttttacatgc gtaaaattaa agggttacgt tggtatatga tcgcactggt
      121 gacgctcggc accgtgcttg gttacctgac gcgtaacact gtggcggcag ctgcgccaac
      181 tctgatggaa gagttaaaca tctccaccca acagtattcc tatatcatcg cagcctattc
      241 tgctgcttat acggtcatgc aaccggtagc aggttatgta ctggatgtgc tgggtacgaa
      301 aatcggttat gcaatgtttg ctgtgctgtg ggccgtgttc tgtggtgcaa ccgcgctggc
      361 aggtagctgg ggtggcctag ctgttgctcg tggtgcggtc ggtgccgcgg aagcagcgat
      421 gatcccggcg ggtctgaaag ccagctccga atggttcccg gcgaaagagc gttccatcgc
      481 agtaggctac tttaacgtag gttcttcgat tggtgcgatg attgcgccgc cgctggtggt
      541 atgggcaatc gtgatgcaca gctggcagat ggcatttatc atctccggtg cattgagctt
      601 tatctgggcg atggcatggc tgattttcta taaacatccg cgcgaccaga agcatctgac
      661 cgatgaagaa cgcgactata ttattaatgg tcaggaagcc cagcaccagg tgagcacggc
      721 gaagaaaatg tccgttggtc agatcctgcg taaccgtcag ttctggggta tcgcactgcc
      781 gcgtttcctt gcagaaccgg cctggggtac ttttaacgcg tggatcccgc tgttcatgtt
      841 taaagtttac ggctttaacc tgaaagagat cgcgatgttc gcctggatgc cgatgctgtt
      901 tgctgacctc ggttgtatcc tcggtggtta cctgccgccg ctgttccagc gttggtttgg
      961 tgtgaacctg atcgtttccc gtaagatggt cgtaacgctg ggtgctgtgc tgatgattgg
     1021 cccgggtatg atcggtctgt tcaccaaccc gtatgtcgca attatgctgc tgtgtatcgg
     1081 tggttttgcc caccaggcac tgtctggcgc gctgattacg ctctcttctg acgtgttcgg
     1141 tcgtaacgaa gtggcgacgg cgaacggttt aaccgggatg tccgcatggc tggcaagtac
     1201 gctgtttgcg ctggttgtcg gtgcactggc tgacaccatc ggcttcagcc cgctgttcgc
     1261 agttctggca gtatttgacc tgctgggtgc gctggttatc tggaccgtat tgcagaacaa
     1321 accggcgatt gaggtggcgc aggaaacaca taacgatccc gcaccgcaac attaacgttt
     1381 aacgtaagtt ataacgacga agccgcctta tcaggcggct tcgtcgttta tatagaaaaa
     1441 agcgtttgtc cggtttgcag ggttaattga tggttggcaa ggaaggcgta aagattactg
     1501 acaatttgcc catgtagtac ccggcctgtt tatccatcac gctgaattta tcgactgcca
     1561 gagtcaaggg tgtcggcaca cacatcacgg catagcgaat accgggcagt accaccggac
     1621 gcacacgttt caggttaatg ctgttccaga ccatattttc attattgcgc acatcaattt
     1681 gcccaccggt ttccgggttg tacattttga cccggtaatc aatgcgttct ccctcgtcgg
     1741 tggtggtccc tccctgacga tagatagaga agtaaccgtc tctgcgccct gcagctggtc
     1801 gcccttcatc tttgagcatg atttcataac tgatggcatt accgttaaaa ccatcgtaca
     1861 ggcacatgtc caacatggtc agatcttgcg cgtagttgct gccgttaacg cttcctgttg
     1921 gatgcaagtc taactgcacc cttggcgtgg cgtgggcgaa ctccgggaag aacacgtcaa
     1981 tatggttggg gtcgagcgta ttcagggtaa tatttgcgaa atagtcctgg gccgggctgt
     2041 tcgaatgtaa tttaactttc cccttccaga cgccaccaat cggcaaattc ttcatctctg
     2101 tctgctcaag gtagagcgta agaaactttg tatcgtatat ataataatgt tgaccatctt
     2161 ctgtacatga agatctatta gaatccttaa gattcgtctg aagccctttg ccccccttgc
     2221 atcctagcgg cgcttcatcg ctacttacaa tggctgtttt aaatcctttg agttttaccg
     2281 gccaacgcat cccactgcgt tgttcagtta aatagatttg tactgtcgat atatttccat
     2341 tagctgtaca ggtttgttgg aggtcaggat acgaacattc ataccacata tatccattat
     2401 ttcctgccga cgcttcatcc ttccagaagt aataatccgc tctaccagat gccaaatcat
     2461 acgtaatttc actggtcacg tcaggagctg cctgaacgcc agtgagcgtg ccaaacaagc
     2521 ccaatatcgc cgcaatcaat cgatttctca tggtgcaacc tccctttgcg ccgtctgaac
     2581 gtttccacgt aatttaggct gtttcaactg tgcctgaacc tgaatcgact ccggtaattg
     2641 tgaataagtc accacactac aagtaacatc tccgctgtaa cgaatgctgg cgcgtttctt
     2701 tacaacactc agtggacact taaggaattg ctgcccggaa agcagataga gctctttttc
     2761 gctgccgtta atttcaagtg taaatccgcc atcttcagct accagtaaca tggcgctgtt
     2821 cagccccaca atcgggaatt ttttcaccgc tgccggcagt aataaacgac cgatatagtt
     2881 gtagttaaag ctggcagaca cttcgcgata gcgcagcttg ccaggcaaca acattaattc
     2941 acgattccct gcaccgttaa tgatgtttgt tgtcccctgc tgattgccct ggctactgtc
     3001 gttaacctca acaaaaccag actgaaatcc aggataaggg aacaacacac tgtcactttg
     3061 accaagctgc aaagttcggc tgcctgccac ccgcgcttcg agttgcagat cctgactctc
     3121 ttcatcctgt tcagagaagc ctttcacatc caccagtacc gcacccagca aatcgtctgt
     3181 accactggct cccaaattaa cgccatagcg actgacagcc agcgtagagc tataagtgcc
     3241 cgtaaaggcc gaaagatgat catgattctt acggtcataa ctatcagata cggtagcgta
     3301 gacatttccg tattgcccat cccaacgccc gttaaccgcg ctgtttatgg tatcggtgtt
     3361 caggcctccg acggttacgc caagttcttt atggctgaat gaatcgttat aaaaggtatg
     3421 cgataactgc catgacgttt gatcgccatc ctgttcgcta taacgataat ccgtaggcac
     3481 attggttgaa tggctgttgt tattgctatc catcgttggc gtgtcagata acgaaaacga
     3541 taagtacaag ccgttgtcgt cgttatcgcc accgttgtca ttacgatgga aaacaccgac
     3601 tgacgtactc aaaatccagt cacgcatcac aatagcgcgg gaagcgctaa gttgtacagt
     3661 ttcagagata ctttgcgctg ttgtctggcg aagatagtta ttttcaaagc tctgctgttt
     3721 atcccaacgc aaactatcat cagtgttatt ttgtgtgcag gtataaccca gcatcatatt
     3781 ccagccgaca aaattcagtg aaactgtcgc ggaaatactt tcataacagc ttaaggcgtt
     3841 atagctctct ctgctgtcgg ttgtacaagc atcgccgtca gagttggtcc gataaaagcc
     3901 caacgtcggc catcccggat tactccagtt ggcctgttgc atatcacctt tgccgccgtc
     3961 atcgttacgg aacacgctgg cgctgattgc aagattcccc accctgccta aatctgccgt
     4021 ccagttatta cctaactcga aagcactcac atcatcagca ttcgccagcc ctgcgtagag
     4081 ctcatattgc ggatgtaatg gcaggcgtac tcctagctga taagctgaac tttcatcatc
     4141 agaaacctgt gatgtagttt tacctgcctg taagaaccat tgcgcatttc cgtcagtcag
     4201 accgccggtt ttggtaaacg gcacaagctc ggtgcgggtg agttggttat tttcgtagac
     4261 ttttaatgcc acgctgtagc tgcctggcgg aaaggaactg gtatcaataa attgcgaacc
     4321 actattgaga taaaacgatc ccaaaagttg ctcattacga taagcgtcaa cacgagaatt
     4381 gcgcgaaagc agaaccatta ctggggttcc ttgctggctt tgcgcctggt ttaaatagct
     4441 cagtgtcgac ccgatacgca tcccatcgat tgcaccgagt ggcagaaagt taaaggtaaa
     4501 gttcccgcct tgtgcattaa atagtgtgcg gttgtccatg cgacccgcct gcacataata
     4561 acgacgtaaa aaatcataac gataatagag atcgctgacg tcagtattac tgtcactgac
     4621 atcatctgca tcataaccgt tgaaa
//
LOCUS       DAZXUK010000267         4643 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZXUK010000267 DAZXUK010000000
VERSION     DAZXUK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.267"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..520
                     /locus_tag="ACH3L8_003611"
     CDS             <1..520
                     /locus_tag="ACH3L8_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFT4086550.1"
                     /translation="FHWLSAIIVYGMFALGLWMVTLSYYDGWYHKAPELHKSIGILLM
                     MGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLFAIGISGYLISTADGKP
                     ISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHGFMALKHHFIDKDDTLK
                     RMLGKSSSDYGV"
     gene            524..1099
                     /locus_tag="ACH3L8_003612"
     CDS             524..1099
                     /locus_tag="ACH3L8_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFT4086551.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1141..2193)
                     /locus_tag="ACH3L8_003613"
     CDS             complement(1141..2193)
                     /locus_tag="ACH3L8_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFT4086552.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2418..3338
                     /gene="lpxL"
                     /locus_tag="ACH3L8_003614"
     CDS             2418..3338
                     /gene="lpxL"
                     /locus_tag="ACH3L8_003614"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFT4086553.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3510..3695
                     /locus_tag="ACH3L8_003615"
                     /pseudo
     CDS             3510..3695
                     /locus_tag="ACH3L8_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3731..>4643
                     /locus_tag="ACH3L8_003616"
     CDS             3731..>4643
                     /locus_tag="ACH3L8_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFT4086554.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIK"
BASE COUNT         1188 a         1115 c         1133 g         1207 t
ORIGIN      
        1 cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc tgtggatggt
       61 cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata aaagcatcgg
      121 tattttgtta atgatggggc tagttattcg cgttctgtgg cgtgtcatat ctccaccgcc
      181 gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaagctg gacatctcgc
      241 attgtgccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa ctgccgatgg
      301 taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg acgctggcgc
      361 acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg tagtactgtc
      421 tgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg acactctgaa
      481 gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa aaagcctgct
      541 tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg ccgattacaa
      601 aattgacaaa gaaggtcagc acgcctttgt taacttccgc atacagcatc ttggctatag
      661 ctggttatat ggcaccttta aagatttcga cggtactttt acctttgatg aaaaaaatcc
      721 ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta accacgccga
      781 acgcgataaa catcttcgca gtgcggattt tctcaatacc acaaaatatc cacaggcaac
      841 attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg gcgatctgac
      901 gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc agggcgacga
      961 cccatggggc ggtaaacgtg caggctttga ggccgaaggc aaaattaagc tcaaagactt
     1021 caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta tttcagtgga
     1081 aggcgtacag cagaagtaag agtgaaatgt tgccggatgg cacgcatccg gcaatgatat
     1141 ttattctgtt ggatcaggaa tacccagtgt tgtattcaga cgtccacgag acttattaaa
     1201 gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg gcggtaacgc
     1261 gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc atactggaca
     1321 ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt cgcacggcgc
     1381 gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca tccgctcgtc
     1441 aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg ccttacgggc
     1501 gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat gtttcatcca
     1561 ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt ttttatcttt
     1621 atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat ctgccggaat
     1681 ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca tgtcgataaa
     1741 tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat actcaccaac
     1801 attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat cgcgtacctt
     1861 catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca ggcgtaaacc
     1921 ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat tgctcgcagg
     1981 tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa aaacattcag
     2041 cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga tgtggaaata
     2101 cttgtaaaac gaaatggtgg ttcgcggttc gctctcagcc aacattttgg cttttaacgc
     2161 gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg agatgggaaa
     2221 gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacat attttacgct
     2281 acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc ccagtcttca
     2341 gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc ccgtataggt
     2401 tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg cttcatccgc
     2461 gttattggtt aacctggttg ggtattggcg tactttggtt ggtcgtgcaa ttgccctacc
     2521 cgattatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt atgaaacgac
     2581 gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc gaacaagaac
     2641 gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggcctgatg gaaaccggta
     2701 tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc ggcatggaac
     2761 acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc cattttctga
     2821 cactggagct gggcgcgcgg cagtttggta tgcaggaacc ggggattggc gtttaccgcc
     2881 cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc tcaaataaat
     2941 cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa ggcgaagtgg
     3001 tctggtacgc accagatcat gattacggcc cgcgctcaag cgttttcgtc ccgttatttg
     3061 ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg tccggcgcat
     3121 gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa ttgattatgc
     3181 tgccaccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg tggatgaaca
     3241 aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta caccgtcgct
     3301 ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccatgcc ggatgcttca
     3361 gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa gctctctgga
     3421 ttgcgccccc tggaagtcgg gcgcataatt agtgtgttta tcttttcttc ttatgttcac
     3481 cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tgaaaatgac acccctataa
     3541 actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggg gccgccttca
     3601 gtctggtaat gcccttctta cccctctacg ttgagcagct tggtgttacc ggccactccg
     3661 ccctgaatat gtggtccggt attgtcttca gtataatggg ttcctaaaca catcatttta
     3721 ggtattgaat atgagacggg caatcagcta catcagattt tcaagtgaac gacaactgaa
     3781 aggggactct gtacgccgac aatctaagct ggttactgac tggcttgata agaaccctga
     3841 gttttacttg gattcatcct tgagctttaa agatttgggg aaaagtgctt ttagtggtaa
     3901 gcacctgaaa ggtggtctag gtgacttttt aaccgctatc gagaaagggc ttgttaaagc
     3961 tggtgatacg ctactaatcg agagtttaga ccgtctgtca cgtcaggata ttgatattgc
     4021 ttctgagcta ttacgccgta tcttaagggc tggtgtagat gtagtcacac tgagcgatgg
     4081 agaacattac acacgagaaa gtttaaaaga cccgttagcc ctgattaaaa gcattctgat
     4141 aatgcagcgg gcgcatgagg aaagtttgag aaagtctgag cgtgtccaag ctgcatggaa
     4201 ccgtaaaaaa gaactcatct ccgaaggtat caaggtatct aggcgttgtc ctgcatggtt
     4261 aaggctgaat gatgaccgtc gaacattcac aatcattcct gataaagtgg aagttgttaa
     4321 acgagcattt gacctgagat tacagggttt aagcttttgg gctatcacca gaactttaaa
     4381 cgatgaaggt cacttgtcat tgaatcaata cacacccaaa caaaaggggt ggtctgatac
     4441 tgctgtaaag aaactgctca gaaatcgtgc tgtgattgga tgtttcacac ctgctggacg
     4501 tgaagaagtt cagggttact accccgcaat aatcagtgag tcactattct atcgagtaca
     4561 gcaactgaac acagggcaat atggtagggc ttctgtatca agtaatccgc tatcagtcaa
     4621 cttgttcagg ggtatcatca aat
//
LOCUS       DAZXUK010000268         4636 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZXUK010000268 DAZXUK010000000
VERSION     DAZXUK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.268"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..311
                     /locus_tag="ACH3L8_003617"
     CDS             <1..311
                     /locus_tag="ACH3L8_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFT4086555.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="ACH3L8_003618"
     CDS             468..1217
                     /locus_tag="ACH3L8_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFT4086556.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="ACH3L8_003619"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="ACH3L8_003619"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFT4086557.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACH3L8_003620"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACH3L8_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFT4086558.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="ACH3L8_003621"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="ACH3L8_003621"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFT4086559.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..4557
                     /locus_tag="ACH3L8_003622"
     CDS             4084..4557
                     /locus_tag="ACH3L8_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFT4086560.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1239 a         1162 c         1137 g         1098 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gttattgtac atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt
     4441 gacagtctcg ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc
     4501 attacccgtc tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg
     4561 ggatacttta tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc
     4621 aggccgttac cccaat
//
LOCUS       DAZXUK010000269         4632 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZXUK010000269 DAZXUK010000000
VERSION     DAZXUK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.269"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..371
                     /gene="bglB"
                     /locus_tag="ACH3L8_003623"
     CDS             <1..371
                     /gene="bglB"
                     /locus_tag="ACH3L8_003623"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFT4086561.1"
                     /translation="RVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLN
                     DHLVQVNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTR
                     KKSFGWYAEVIKTRGLSLKK"
     gene            457..2073
                     /gene="bglH"
                     /locus_tag="ACH3L8_003624"
     CDS             457..2073
                     /gene="bglH"
                     /locus_tag="ACH3L8_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFT4086562.1"
                     /translation="MFRQNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            2100..3275
                     /locus_tag="ACH3L8_003625"
     CDS             2100..3275
                     /locus_tag="ACH3L8_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFT4086563.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            3277..3999
                     /locus_tag="ACH3L8_003626"
     CDS             3277..3999
                     /locus_tag="ACH3L8_003626"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFT4086564.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(4061..>4632)
                     /gene="cbrC"
                     /locus_tag="ACH3L8_003627"
     CDS             complement(4061..>4632)
                     /gene="cbrC"
                     /locus_tag="ACH3L8_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFT4086565.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
BASE COUNT         1284 a         1026 c         1131 g         1191 t
ORIGIN      
        1 tacgggttct gttaaatacg ctttgggatc gttatcaaaa accgttattt attgtcgaga
       61 atggattagg cgtaaaagac agcgttgaag cggatggttc gatacaggac gattatcgaa
      121 ttgcctattt aaacgatcac ctggtacagg taaatgaagc gattgccgat ggtgtggata
      181 ttatggggta caccagttgg gggccaattg atttagtcag tgcatctcat tcacaaatgt
      241 ctaagcgcta cggctttatt tatgtggatc gtgatgataa tggcgaagga agcctcacaa
      301 gaacgcgtaa gaaaagcttc ggatggtatg cagaagtgat caaaacgcgg gggctgtcat
      361 taaaaaaata accaataaag caccttaatt atcgtcgcat tcagaacagt ctggatgcga
      421 tacgttaatt ctttctttgc accataaagg gatattatgt ttagacaaaa tcttattacc
      481 tctgccatct tattaatggc accgttagcc ttttctgcac aatcattggc tgaatcatta
      541 acggtggaac aacgccttga gttattagaa aaagcgttaa gagaaacgca aagcgaactc
      601 aaaaagtata aagatgaaga gaagaaaaaa tatacgccag cgacggtgaa tcgtagcgta
      661 agtacgaatg atcaagggta tgccgccaat ccgttcccga ccagtagtgc cgcaaaacct
      721 gatgctgtac tggtcaaaaa tgaagagaaa aatgccagtg agacaggctc gatttattct
      781 tccatgactc tgaaagattt cagtaagttt gtgaaagatg aaattggctt tagttacaac
      841 ggctattacc gttctggttg ggggaccgcc tctcatggtt cacctaaatc atgggcgatt
      901 ggttctctgg gccgctttgg taacgaatac tccggctggt ttgatttgca gttaaaacaa
      961 cgtgtctaca acgaaaacgg caaacgggtt gatgccgttg tgatgataga tggtaacgtt
     1021 ggtcagcagt actctaccgg ctggtttggc gataatgccg gtggcgagaa ctttatgcag
     1081 ttctccgata tgtacgttac caccaaaggt ttcctgccct ttgcgccaga ggctgatttc
     1141 tgggtgggta aacacggtgc gccgaaaatt gaaatccaga tgctggactg gaaaacgcag
     1201 cgtactgatg ctgcagcggg tgtaggtctg gaaaactgga aagtcggtcc gggtaaaatt
     1261 gatatcgcgc tggttcgcga agatgtcgat gattacgatc gcagcctgca aaacaaacag
     1321 cagattaata cccatactat tgatttacgc tataaagata tcccgttatg ggataaagcg
     1381 accttaatgg tgagtggtcg ttatgtcacg gcaaacgaaa gcgcatcgga aaaagataat
     1441 caggataata acgggtatta tgactggaaa gatacctgga tgttcggcac atctttaacg
     1501 cagaaatttg ataaaggtgg cttcaacgaa ttctccttcc tggttgcgaa taactctatc
     1561 gccaggaact ttggccgtta tgctggcgca agtccattta ccacctttaa tggtcgttat
     1621 tatggtgatc acaccggcgg aacagcagtt cgtctgacct cgcagggcga agcctatatc
     1681 ggcgatcatt ttattgtggc taacgcgatt gtttactcct tcggtaacaa tatttatagc
     1741 tacgaaacag gcgcacactc tgatttcgaa tctattcgtg cggttgttcg cccggcctat
     1801 atttgggacc aatataacca gacaggtgtt gaactgggct atttcaccca gcaaaacaaa
     1861 gatgcgaata gtaataaatt taatgagtct ggttataaaa ccacgctctt ccataccttt
     1921 aaagtcaata ccagtatgtt gacctcgcgt ctggaaattc gtttctacgc cacgtatatc
     1981 aaagccctgg aaaacgaact ggatggcttc accttcgaag acaataaaga cgcccagttt
     2041 gctgtcggtg cccaggctga aatctggtgg taatggaact taatgaaagg aatattacga
     2101 tgaatataaa aattgctgct ttaacgctgg ctattgccag cggtatttct gctcagtggg
     2161 ccatagcagc ggatatgcca gccagcccgg cacccactat cccggtgaaa cagtatgtga
     2221 ctcaggtcaa tgccgataac agcgtgacct ttcgctactt tgcccctggg gcaaaaaatg
     2281 tctctgtagt ggtgggtgtt ccggttccgg acaatattca cccgatgacc aaagacgaag
     2341 caggagtctg gtcgtggcgc acacctatcc tgaaaggcaa cctgtacgag tattttttca
     2401 atgttgatgg tgtacgcagc attgatacag gcaccgcaat gactaagcct cagcgccagg
     2461 ttaactccag tatgattctg gtgccaggca gttatctgga tacgcgttct gttgcgcatg
     2521 gtgatttgat cgccataact taccactcca acgcattgca atctgaacgt cagatgtatg
     2581 tctggacccc gccaggatac accggcatgg gcgagccttt gccagtgctc tatttctatc
     2641 acggttttgg tgataccgga cgttccgcta tcgatcaggg gcgtatcccg caaatcatgg
     2701 ataacctgct ggctgaaggg aaaattaaac cgatgctggt ggtgatcccg gataccgaaa
     2761 ccgatgcgaa gggcattatt cccgaagatt tcgtgcctca ggaaagacgt aaagtctttt
     2821 atccgctgaa tgctaaagcg gcagatcgcg aactgatgaa cgatattatc ccgctgatta
     2881 gcaagcgttt tagtgtccgt aaagatgccg atggtcgcgc gctggcaggg ctttcacaag
     2941 gcgggtacca ggcgctggtt tccggaatga atcatctgga aagctttggc tggctggcca
     3001 cattcagtgg tgttaccacg acaaccgtac cggatgaagg tgtcgcggcc cggctgaacg
     3061 atccggcagc tattaaccag caactacgta attttactgt ggtcgttggt gataaagatg
     3121 tcgtaaccgg caaggatatc gccgggctga aaactgagct tgagcagaaa aaaattaagt
     3181 ttgattacca ggaatatccc ggcctgaacc atgaaatgga tgtctggcgg cctgcctatg
     3241 cagccttcgt acagaaattt aaaataaggc attaagatga aattaatcat taccgaagat
     3301 taccaggaaa tgagccgtgt cgcggcacac catctgcttg gttatatgtc gaagacgcgt
     3361 cgtgttaacc tggcaattac cgccggtagc acgcccaaag gcatgtatga atacctcacc
     3421 accctggtta aaggtaagcc ttggtacgat aactgctatt tctataattt cgatgaaatt
     3481 ccctttcgcg gcaaagaggg agaaggcgta acgattacca atctgcgtaa tctgtttttc
     3541 acccctgcgg ggatcaaaga agagaatatc cagaagctca ctattgataa ctaccgcgag
     3601 catgatcaga aactggcgcg tgaaggcggc ctggatttgg tggtgctggg attaggtgca
     3661 gatggtcatt tttgtggcaa tttgccgaat acgacccatt tccatgagca aaccgtggag
     3721 ttcccgatcc agggagagat ggttgatatt gtggcgcatg gtgagttggg cggcgacttt
     3781 tcgttagtgc ctgacagcta cgtgacgatg gggccgaaaa gcatcatggc ggcgaaaaac
     3841 ttgctgataa ttgtcagtgg cgcaggtaaa gcgcaggcac tgaaaaatgt gctgcaaggt
     3901 ccggttaccg aagatgttcc agcttccgtg ttgcagctgc atccttcgct gatggtaatc
     3961 gctgataaag ccgctgccgc agaattagcg ttcggttaag ctgaagaaat aaggccggat
     4021 gtatgaatta cgctccggcc tgttctcatt atttaaataa ctacgaaaaa tcaccccaca
     4081 gtctcagctt gccgcagtgg agacagcgga agagataacc ctgacaatca ccaccctttt
     4141 gcaggcattt agctagatca gaatttctaa taccgaaatt ctcacaatct tcttcaaggt
     4201 tggcaaattc atcgaggcga tcttttatat cattccagcc cacatagccg ataaaagcac
     4261 agaaatcgcc acaatgcgca agccagaatt cctgttgcca tccatgataa cctggcgtgc
     4321 gttcaaccaa ctctttcagc atttcatcag gatatgtgtt cttaataccg gcaaattcgt
     4381 cctcttcatc atactcaaat tcaacacctt ctatgctggc atcatcctga aaactacctg
     4441 caaacttttc tgcagcagaa ccgtccgcaa tacacaacgg acagagatgt tcaacttcat
     4501 caacgcaata aaagggaccc gagtaataaa ctgacgtctg ttgttcacag caatcgcact
     4561 ctacggtttt atcctgttca aatgcgcctg tttccagtgg cttgggatga tatttgaatg
     4621 taagcgcccc ga
//
LOCUS       DAZXUK010000270         4620 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZXUK010000270 DAZXUK010000000
VERSION     DAZXUK010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.270"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..218)
                     /locus_tag="ACH3L8_003628"
     CDS             complement(<1..218)
                     /locus_tag="ACH3L8_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFT4086566.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIV"
     gene            complement(221..940)
                     /gene="sanA"
                     /locus_tag="ACH3L8_003629"
     CDS             complement(221..940)
                     /gene="sanA"
                     /locus_tag="ACH3L8_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFT4086567.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1090..1974)
                     /gene="cdd"
                     /locus_tag="ACH3L8_003630"
     CDS             complement(1090..1974)
                     /gene="cdd"
                     /locus_tag="ACH3L8_003630"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFT4086568.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2104..2799)
                     /locus_tag="ACH3L8_003631"
     CDS             complement(2104..2799)
                     /locus_tag="ACH3L8_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFT4086569.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2796..3194)
                     /locus_tag="ACH3L8_003632"
     CDS             complement(2796..3194)
                     /locus_tag="ACH3L8_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFT4086570.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            3433..4380
                     /gene="dusC"
                     /locus_tag="ACH3L8_003633"
     CDS             3433..4380
                     /gene="dusC"
                     /locus_tag="ACH3L8_003633"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFT4086571.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         1210 a         1177 c         1222 g         1011 t
ORIGIN      
        1 acaatcatgc cgatagataa aattccgaag ccaatatatg ccaccatata agcgaagaag
       61 agtccgggat tttttgctcg ccagacatac accggttccg gtgcattttt aacccgttta
      121 tcaatcgcgc ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat cgggataagg
      181 aaaaaaacgg caacgacaaa aaactgctgt acatccataa ctactttcct tgtttctttt
      241 gtaattcaag taactgttcg ggtgtaacgg cgggataccc ctgcgcatct tccggcacct
      301 ggtgcatagc cggaataggg accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat
      361 aaaggtcagc cagcgcaccg aaacgggcgg caaattcgcg aatacgtact gacagcatat
      421 ctttcggtga cggtacggca taacactgag cctgaatccc catatgcagc gcaataaata
      481 atgctcgctc acagtggaaa cgttgggtga taataatgaa atcattagta tcgaaaactt
      541 tgcgtgtacg cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga acaatatctg
      601 atgggtcgac accagcagcg attaaatctt tgcgcatggt catcggctcg ttataacttt
      661 gcaatgcgtt atcgccgctc agtaatagat aatttacctt accgctgtta taggcattaa
      721 tcgctccttg aatgcggtag cgataatact gattaataac gccggtacga taatattttg
      781 ctgtgccgag caccacgccg acctggcggt aggggagatc ctgcaattcg tcgtagatat
      841 aaggcgcggt tttccagctc atccagcgat cgaggccgag cacagtcaac agcagcaagc
      901 cgatcaggac taacaggctg aggaacacgc gctttaacat gcaggagact cgagctaagg
      961 aaaatttcca tcaggctact ttacccgctg gttttgcgca agaaacggca aactgtttgc
     1021 cgggaatttc gacaccagga gagggctggc aagccggaca aggcgttcac gccgcatccg
     1081 gcaccaggct taagcgagaa gcacgcggtc gatactgtga cagccgagag ctttcagcgt
     1141 tgcggaggtg gcatcccact gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg
     1201 ctggatatcc gggtaatcat aacccttgag attcaacaga attaacgccc cctgcaacgg
     1261 cggcagagtc gggttgaatg cggcgttttc agcgtagctg ccgctgaaaa tacgaccgtc
     1321 tttacattcc agcgcgacac cgcttggtga cttactgtaa ggcatgtgcg aacggtttgc
     1381 cgctgcaatc gccgcctgag aaagcgcatc accagtcagc gcatagccgt gatcctgctc
     1441 gtccatcagc agcgttttaa tctccagatc tttcggccca aaggcatccg gcagatagtc
     1501 acgcagcgcg tgtgcctcgc ggcccggcaa atgaatacgc agatccagac cgctgttcag
     1561 ttcattcata aactgacggc agtgaccaca aggcgtatag ttaacggtga tggctgcaag
     1621 cgctttttca ccactcaacc aagcgtggct gatcgcgctt tgttcggcat gaacggtttg
     1681 ctgcattgtt gcaccaataa attccatatt ggcaccaaaa taccaggttc cgctcacacc
     1741 gcgcgcaatt gcgccaacat taaaattcga caatggcgta cgcgcacagg ccgccgccag
     1801 cggaagtagt gcgaatgcca gcgcgtcttc gtccagtccc gttgcgctct tcagcgatga
     1861 gacttgctcc ccggtcaaca aagcggggaa gtacttgtct gccagaatag gttccagtgc
     1921 agattgcaag ttatccgcaa gttgggcaaa agcggtttga aaacgtggat gcatggcgtt
     1981 gcctcataat catgtaatgg gataacagtt tacgaacccg ttatggcttt atatgtgatc
     2041 tgaatctcat taattatgca acacgtgaaa cttacatcaa aaatgcgcga cgtatcgcaa
     2101 attttagccc attactgcca gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat
     2161 tatcccgcat aacaccagcg ctagcgaact aaatgcacct tcctgataat ccagctcggc
     2221 gcagcgcgcc gtaccgaggg cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt
     2281 ggtacgaata cgcatcgcat taagcaatgt atggccaaat accgcgccga ggatgccgac
     2341 gaaaatcacg caaactgcgc tgattgccgg aataccgcca atactgccgc caaccgccat
     2401 tgcaataggc gtggtgactg atttcggcag gattgacgcg gcgatttccg gtgaagcgcc
     2461 catcaacaat gccacggaag tccccgtcac catcgcaacc acgctgccga taaaacagat
     2521 ggtgatgatc gatttccagc gcgcgcggat ctggtgtagc tgctcatata aaggataggc
     2581 cagcgcgacg accgccggtt gcagcagatc gtttagcact tcgctacctt taaagtaact
     2641 gtcgtaagag atgccggtca gcattaaaaa aggaatgatc accaccattg ctaccagcaa
     2701 cgggttaagc aggggaaact tataccgtgc cgccagtttg cgggcggcaa aaaagacaat
     2761 caacgtcagc ggtaatgacc accagatatt cgccatcatt cttctgatcc tttctgacct
     2821 acgactttac gttcaccgtg taccagttgt gaactccagc tcaccaccag aaaaaccacc
     2881 agcgtactga cagcacagga aaccactacc gggccaaact gcgcgcggag caaatcaaaa
     2941 tattgcatga cgcctacgcc aatcggcaca aacaatagcg ccatatagcg aatcagtacg
     3001 tagcaccccg gattgaccca tttcgccgga agaatttgca aggccagcag gacaaacagg
     3061 atcagcatcc cgatgatgct gcccggaatg gttaccggta gcagggaagc aatgaaaatg
     3121 cctgcataca ggcaggcata aatcaggacg aaagcgcgta aatattgcca gataatgttc
     3181 agtgtcttac tcataatcaa caccccagat gagatggatt catcatacaa ttaattcgta
     3241 aaacgtgcta ccgatcacat taagaaatca gcgcttattt tttcgctacc cggtattacg
     3301 caatgagcag cgaactcagg catacatcaa gcatagagag ggattgcagt ttgcagcaat
     3361 ccctctttgc agacctttaa tccgctgacc ataggcgcta acatagcgcc tcattttttt
     3421 gcgggtgatg atatgcgtgt gttactggca ccgatggagg gagtgcttga ctctctggtg
     3481 cgtgaattgc tgaccgaagt taacgactac gatctgtgca tcaccgagtt tgtccgcgtg
     3541 gtggatcaac tgctgccggt aaaagtcttt catcgcattt gccctgagct acaaaacgcc
     3601 agccggacac catctggtac gctggtgcgc gtgcagttgt taggtcagtt cccacaatgg
     3661 ctggcagaga acgccgcccg tgcggtggag ttaggtttct ggggcgtgga tctcaattgc
     3721 ggctgcccgt cgaaaacggt taacggtagc ggcggcgggg cgacgttact caaagatcct
     3781 gaactcatct accagggggc aaaagcgatg cgtgaagctg taccggcgca tttgcctgtc
     3841 agcgtgaaag tgcgtctggg ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg
     3901 gttcaacagg ctggcgctac ggagctggtg gtgcatgggc ggacgaaaga gcagggttac
     3961 cgcgcggagc atattgactg gcaggcgatt ggcgagattc gccagcggct gaatattccg
     4021 gtgattgcca acggtgaaat ctgggactgg cagagcgcgc aacaatgcat ggcgatcagc
     4081 ggctgcgacg cagtgatgat tggtcgcggg gcgctcaata ttcccaacct gagccgggtg
     4141 gtaaaatata acgaaccgcg aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat
     4201 acccgtctgg aaaagcaggg cgataccggg ttatatcacg ttgcgcggat taaacagtgg
     4261 ttgagttatt tgcgtaaaga atacgatgaa gcaacggaat tatttcagca tgttcgggtg
     4321 ttgaataact cccctgatat tgcaagggct attcaggcaa ttgatatcga gaaactctaa
     4381 aaaccatagt gatgtttttg acaatgtgtt agctctttat caataggccg cacggttgtt
     4441 tggtggcgtc aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg
     4501 gctgagggcg cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata
     4561 agccgagggc accgcgcagc ggcgattttg ttcgccagag cccggggggg cagggggcgg
//
LOCUS       DAZXUK010000271         4606 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZXUK010000271 DAZXUK010000000
VERSION     DAZXUK010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.271"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..4096
                     /gene="pic"
                     /locus_tag="ACH3L8_003634"
     CDS             <1..4096
                     /gene="pic"
                     /locus_tag="ACH3L8_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HFT4086572.1"
                     /translation="YCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPAICLCYSQI
                     SQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPMADFSSVSS
                     NGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPRLNKLVTEV
                     IPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAFKTGGTTGV
                     PLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVAVLRAYAGI
                     NGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLSQGSKNWTM
                     HGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGKTWTGAGII
                     TDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQQADTAGNI
                     QAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQAADYGAVI
                     TNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQTRFYILKSA
                     SYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQLTGNMDVAI
                     PQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLNQKDWENRQ
                     FTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNYVIPEEGTS
                     VPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLTAPGAFAGS
                     SLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYLTDGYDLTG
                     DNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLLEGYNAAFN
                     GAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNKLDATGSDF
                     VLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGTSAEMFKAG
                     TRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYKNFMTEVNN
                     LNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELDGVDLFTGV
                     TMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDYTGNFASLG
                     TKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDMDLSMKNRD
                     FSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEKRIKGEKDS
                     RMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4247..4489)
                     /locus_tag="ACH3L8_003635"
                     /pseudo
     CDS             complement(4247..4489)
                     /locus_tag="ACH3L8_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         1219 a         1055 c         1248 g         1084 t
ORIGIN      
        1 atattgcccc gtcaccgggg ggcttattgc tgtctctgaa cttgcccgca gggtaataaa
       61 aaagacatgc cgaagattaa cgcatattct tctggctggc attccagcaa tctgtctgtg
      121 ttactctcag atatcccagg cgggtattgt ccgttccgat attgcctatc agatttatcg
      181 tgatttcgcc gaaaacaaag ggctttttgt acctggtgcc aatgatattc cggtatatga
      241 taaggacgga aaacttgtgg gaagactggg taaagcccca atggccgatt tcagcagtgt
      301 gagctcaaat ggcgttgcta cgcttgtatc gcctcagtat atcgtcagcg taaagcataa
      361 cggaggatat cggagtgtga gctttggtaa tgggaaaaat acatattccc ttgttgaccg
      421 taataaccac ccttctattg acttccatgc tccacgtctg aataaactgg ttacagaagt
      481 tattccctca gcggtaacat cagaaggaac caaagccaat gcttataaat acactgaacg
      541 ttacaccgct ttttatcggg tgggtagtgg tacgcagtac actaaggaca aggacggaaa
      601 tttagttaag gttgccggtg gatatgcttt taaaacagga ggaaccacag gagttcctct
      661 gatatctgat gcaacaatag tctctaatcc cgggcaaact tataatcctg taaacggccc
      721 tttacctgac tatggagccc ctggggatag tggttctcct ttgtttgctt atgataaaca
      781 acaaaaaaaa tgggttattg ttgctgtatt aagagcatat gcaggtatta atggtgctac
      841 gaactggtgg aatgtcatac caacagatta tctgaaccag gttatgcagg acgatttcga
      901 tgcccccgta gactttgttt ccggactggg ccccctgaac tggacatacg acaaaacatc
      961 aggcacaggt accctgagcc agggcagtaa aaactggacc atgcacgggc agaaagacaa
     1021 tgacctcaat gccggtaaaa atctggtatt cagcgggcag aatggtgcaa ttatcctgaa
     1081 agacagtgtg actcagggtg ccggttatct cgaatttaaa gacagttaca ccgtatctgc
     1141 tgaatccgga aaaacatgga cgggtgccgg cattattact gacaagggga cgaatgtaac
     1201 ctggaaggtc aacggcgttg ccggtgacaa cttgcataag ctgggggaag gaaccctgac
     1261 cataaacgga acaggtgtaa acccgggagg actgaaaacg ggagacggta tcgttgtact
     1321 taaccagcag gcagacactg caggtaatat ccaggccttc agttcagtga acctcgccag
     1381 cggacgtccg accgtggtgc tcggggatgc ccgtcaggtc aatccggata acatttcatg
     1441 gggataccgg ggaggtaagc ttgaccttaa tggtaatgcc gttaccttca cccgactgca
     1501 ggctgctgat tacggggcgg tgattacaaa taatgcacag caaaaatccc agcttttact
     1561 ggatcttaag gctcaggata caaatgtcag tgaaccgacg attggaaata tatccccctt
     1621 tggtggtacc ggcactccag gaaacctgta cagcatgata ctcaacagcc agacccgctt
     1681 ctatattctg aaatctgcca gctatggtaa cactctgtgg gggaacagcc tgaatgatcc
     1741 ggctcagtgg gagtttgttg gcatgaacaa aaacaaagca gttcagacag taaaagatag
     1801 gatcctggcc gggcgggcaa aacaacccgt tatctttcat ggtcagctga ccgggaatat
     1861 ggatgtcgcc attccacagg tgccgggggg aagaaaggta atctttgatg gtagcgtgaa
     1921 cctgccggaa ggtaccctga gtcaggacag tggcaccctg atattccagg gacatccggt
     1981 tatccatgcc tccatcagtg gcagtgcacc ggtcagcctg aaccagaaag actgggaaaa
     2041 ccgtcagttt acaatgaaaa cactgtcgct gaaagacgct gacttccatc tttcacgtaa
     2101 cgcctcgctg aacagtgaca ttaagtcgga taacagccat atcacactgg gaagtgacag
     2161 ggcatttgtg gataaaaatg acggaacagg aaattatgtc attccggagg aaggtacctc
     2221 tgtcccggac accgtgaatg acaggagcca gtatgaaggg aatattacgc tgaaccataa
     2281 ctcagccctg gatatcggca gcaggttcac cggggggatt gacgcttatg acagtgccgt
     2341 cagcatcacc tctccggacg tcctgttgac agccccgggt gcttttgccg gcagttcact
     2401 gacagtgcat gatggcggtc atcttacagc actgaacggt cttttcagcg acgggcatat
     2461 tcaggccggt aagaacggca aaatcaccct gagcggtaca ccggttaaag atacggctaa
     2521 tcagtatgcc cctgctgtat atctgacgga cggatatgac ctgaccggcg ataacgcagc
     2581 actggaaatt acccgtggag cacatgcttc cggtgatatt catgcctctg cggcatcaac
     2641 agttaccatc gggtctgaca cgccggcaga actggcttct gcggaaacgg ctgcatcggc
     2701 gtttgccggc agtcttcttg agggctataa cgcagcattc aatggtgcca taaccggtgg
     2761 cagggctgat gtcagtatgc ataatgcact gtggactctg ggtggggact ctgccatcca
     2821 cagtcttacc gtcagaaaca gccgtattag ttctgaagga gaccgtacat tccgtaccct
     2881 gacggtgaat aaactggatg caacaggcag tgattttgtt ttgcgtacgg acctgaaaaa
     2941 tgccgataaa attaatgtga ctgaaaaagc cactggttca gataacagcc tgaacgtcag
     3001 ctttatgaat aatcctgctc agggacaggc cctgaatatt cctctggtca cggcaccggc
     3061 gggaacttca gcagagatgt ttaaggccgg cacccgggtg acaggtttca gtcgggtgac
     3121 cccaaccctg catgttgata ccagtggtgg caatacgaag tggatactgg atggttttaa
     3181 agcggaggct gataaagccg ctgccgcgaa ggctgacagt ttcatgaatg ccgggtataa
     3241 aaacttcatg acggaagtta acaatctgaa caaacgtatg ggtgacctgc gtgacacaaa
     3301 cggtgatgcc ggtgcctggg cgcgcatcat gagtggtgcc ggttctgcag acggtggtta
     3361 cagtgataat tacacccatg ttcaggtcgg ctttgacaaa aaacatgaac tggacggtgt
     3421 ggacctgttt accggtgtca cgatgaccta taccgacagc agtgcagaca gccatgcatt
     3481 cagcggaaag acgaaatcgg tggggggcgg tctgtatgct tcagcattgt ttgagtccgg
     3541 tgcctatatc gatttgattg gtaaatatat tcaccatgac aatgattaca caggtaactt
     3601 tgctagcctg ggaacgaaac actacaacac ccattcctgg tatgccggtg ctgaaacggg
     3661 ttaccgctat cacctgacag aggacacgtt cattgagccg caggctgaac tggtttacgg
     3721 cgccgtgtcc gggaaaacat tccgctggaa agacggtgat atggacctga gcatgaagaa
     3781 cagggacttc agtccgctgg ttggaagaac aggggttgaa ctgggcaaga ccttcagtgg
     3841 taaggactgg agtgtgacgg cccgtgccgg aaccagctgg cagtttgacc tgctgaataa
     3901 tggagagacc gtactgcgtg atgcgtccgg ggagaaacgg ataaaaggag agaaggacag
     3961 ccggatgctg tttaatgttg gtatgaatgc gcagataaag gacaatatgc gctttggtct
     4021 ggagtttgag aagtcagcct ttggtaaata taacgtggat aatgcggtaa acgcgaattt
     4081 ccggtatatg ttctgatgtg ggggtaaagg gctgccggaa ggcagccttt ctcagagact
     4141 cttaaaatat aaaactgaag gtcttcggta agggggagtg tattagacgt caactagttt
     4201 tgccgactgg cgcgagcctg tttaagattc tgtgtaaatg cctttctcag aagtgaccgt
     4261 ccaggcggtc accgaactcg ataataaagc ggctcattgc cattcgccag tccttcaacg
     4321 gcatcgtcca tttctgggac gcagactgga ttgccagcca caccactttt ttcaccgagt
     4381 cgtctgtcgg gaacacttta cgctttttga tcgcatggcg gatcacgctg tttagcgact
     4441 cgatggcatt cgtcgtatag atcactttgc ggatgtccgt tggataagcg gtaagcgtac
     4501 agcgagggcc gtattgacgg ggatgtgtta ttcagctggc agtgctatgc gccacggaag
     4561 cagttcgctg acccggttga ccggccagtc tgctatgacg ccaagc
//
LOCUS       DAZXUK010000272         4604 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZXUK010000272 DAZXUK010000000
VERSION     DAZXUK010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.272"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(98..766)
                     /gene="artM"
                     /locus_tag="ACH3L8_003636"
     CDS             complement(98..766)
                     /gene="artM"
                     /locus_tag="ACH3L8_003636"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFT4086573.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(766..1482)
                     /gene="artQ"
                     /locus_tag="ACH3L8_003637"
     CDS             complement(766..1482)
                     /gene="artQ"
                     /locus_tag="ACH3L8_003637"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFT4086574.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1489..2220)
                     /gene="artI"
                     /locus_tag="ACH3L8_003638"
     CDS             complement(1489..2220)
                     /gene="artI"
                     /locus_tag="ACH3L8_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFT4086575.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2238..2966)
                     /gene="artP"
                     /locus_tag="ACH3L8_003639"
     CDS             complement(2238..2966)
                     /gene="artP"
                     /locus_tag="ACH3L8_003639"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFT4086576.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3184..3699)
                     /locus_tag="ACH3L8_003640"
     CDS             complement(3184..3699)
                     /locus_tag="ACH3L8_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFT4086577.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDTRNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            4348..>4604
                     /locus_tag="ACH3L8_003641"
     CDS             4348..>4604
                     /locus_tag="ACH3L8_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFT4086578.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFS"
BASE COUNT         1215 a         1120 c         1110 g         1159 t
ORIGIN      
        1 acgccgcatc cggcacaggc accgtgctga tgtctgatgc gacgctggcg cgtcttatca
       61 gacctacaaa cccccccggc gaatgtacgc agctacatta attccgccgt tcgaatgcca
      121 gtgctttgcg ctcgatcaga cgcatcatca gcgtcagcag gccgttgacg accaggtaaa
      181 taatccctgc cgcaccgaac accattacat cgtaggtgcg tccgtacaac aactggctgt
      241 atcccatcac ttccatcagc gtaatggtgt aagccagaga ggtacttttg aacaccagca
      301 ccacttcgtt ggaataagaa gagagcgagc gtttaaaggc atacggcagc aggatcgcca
      361 gcgtatcttt tttgctcatt cccagggcgc tacaggactg ccactgacct tctgggatcg
      421 cacgaattgc accgtaaaac agctgcgtgg tatacgccgc actgttcagc gataacgcaa
      481 tcagcgcaca taaccacggt tctgacaaca aatgccacag tgccggatac tcctgcaacg
      541 tcggaaactg gcccggcccg taataaatca ggaagatctg caccagcagc ggcgtaccgg
      601 taaacagcgt gatataaccc cgcaccagcc acaccagcac cggcgttttc agcgtcagga
      661 tgatggtaaa aatcaatgcc agaatcagtg ccacaatcag cgaggcaacg gttagcgtca
      721 ggctggtgtg cagccctttc atcagctcgg gtaaatactc aaacattagc tgggcctccg
      781 ctcaaaacgt gtcgcgcgca ggtcaatgcg tttgagaatg tactgactga gcagggtgat
      841 caccaggtaa atcgccgccg ccacaatgta ccaggtaaat ggttcctggg tacgagtagc
      901 aatgcttttg gtttgtaaca ttaaatcatt cacactaatc aaactgacca gcgcggtatc
      961 tttcagcagc accagccact ggttaccgag gccaggcagc gcatgacgcc acatctgcgg
     1021 catcaccaga cggaaaaaga tagccgattt cgacagcccc agcgcctgac cagattccca
     1081 ctggcccacc ggtacagctt tcaacgcgcc gcgcagcgtt tgcgaggcat aggcggcata
     1141 cagcagtgac agagcgatga caccacaaag gaacggactc acgtcgaagt tctcaatgtc
     1201 catctgcacc gggatctgca cgaacccaag attgatagtg aagccatccg aaagcgtcag
     1261 cagtaactgc gaggagccaa aatagataaa cagcactacc agaatttctg gcaggccacg
     1321 cagaatggtt accagcgctg aacctgccca cgcgacagga cgccatttcg ctgactccca
     1381 taccgcaaag aacatcgcca gcgccagccc gacaatcaat gcacaaacgg caaggccgac
     1441 ggtcatcccg gcggcgcttg ctaaaggaaa aaattcattc atcaggaatt acttctggaa
     1501 ccatttgttg tagatggttt cgtaagtgcc atctttcttc actttttcca gcgcagtgtt
     1561 gagtttctgc tgcagttcag tgtttccctg acgtaccgcg atgccgaggc cagtaccgaa
     1621 ataatcttta tcggtcactt tatcgcccac tgccgccagt tttgggttat ctttcagcca
     1681 ctcagtgacc actgcggtgt caccaaatac gctgtcgata cgcccgtttt gcagatccag
     1741 ttttgcgttc tggtagctgt catacggaac ggtggtgatt tccgggtgct tatccataat
     1801 gaacttctgg tgtgtcgtcc cgttctgaac gccgactttt ttgcctttca gctgatcaac
     1861 actggtgtat ttgccttgct gacccacaaa cagggcagag ttgtcatagt acggggtagt
     1921 aaacagtacc tgcttttcac gctccggagt gatatccatg cccgccatca ctgcttctac
     1981 gcgacggaat ttcaggcttg ggatcaggct gtcaaacgcc tggttagaga aagtacaggt
     2041 tgcatcaatc tctttacaca gtgcctgcgc caggtcgacg tcaaaaccaa cgatctggtt
     2101 gtttgcatca atcgattcaa acggaggata gggggcttcg gtagcaaaac gaatggtttc
     2161 ggcagctgtg gcggaaagac taaaacctgc aattaacgcg gcaatcagaa cttttttcat
     2221 tgttgttatc ccgaatctta gtgagagaga tagtttttaa atgcttcggt ttgcggctcg
     2281 gtaaagcagc tcgcgtcgcc ttgttctacg atatgaccat tttccatata caccactcgg
     2341 ctggcggttt tacgcgccac ttcaacttcg tgggtgacga tcacctgggt aatattcgtt
     2401 tctgccagct cacgaatgat gctgacgatt tgtgccgtaa tttccgggtc cagtgcggcg
     2461 gtcggttcat cgaacagcag tacctgcggt tccatcatca acgcacgggc aatagcaaca
     2521 cgctgctgct gaccaccaga aagatgcagc gggtaacgat cgctataagg tttgagacgc
     2581 agacgttcca gcagtttttc tgcacgggcc agcgcctgat ctttactcaa ccccagtaca
     2641 cggcagggcg cttcaatcag gttttgctgc acggtcagat gcggccacag gttgtattgc
     2701 tgaaacacca tgccaacgtt acgacgcaaa tcgcgaatcg ctttgtcaga gggtgttttg
     2761 gtgaaatcga aatggttgcc tgcaatgttg agcgtaccgg agcgcggcat ctcaagcaga
     2821 ttgagtacac gcagcagcga gcttttaccc gcgccgctgg ggccaagtaa caccagcgtt
     2881 tcgccctgtg ggcaatccag cgtgatatcg aacagcgcct gatgcgcgcc gtagaagcaa
     2941 ttaatgccgt ttaattgaat actcattgac actcgtatac tggcagtctg atagctattg
     3001 aggtcgaaga tagtaccttt gacagaataa ttatgcaata tttatgcttt aaaagttaaa
     3061 agcaaagcgc attattcaat aaacatagca caaaataacg ggggcggtgg tcggtgagca
     3121 taaatgtcgg cattcctcac gaaatgccgg ataatttacg gggtttattg gttgatcaag
     3181 gcgttagcga ttctcgatgg actgacggag cgtacccgcc gtggcatgaa cgctaccgcc
     3241 taagtagcgc acatcgtcga tgacccaaca ctggccttcc tgaatcatta acacttcatc
     3301 ctgccagccc tggtcaccct gtttgagatc cacccgcaat ggaatgttac gggtatcacg
     3361 attagggata gtcgatgcac tggcaacgtg ggcgctgtct ggcaaggtgg ttcgactgga
     3421 gaacggatcg ctgcttaaca attcacgatg actattatcg cggctggcat cgctaagttg
     3481 ggttgccagt ttgtcactca aataggggcg cagggcggta atgtcattgc tgcgatgttg
     3541 aatgcggtag tcgtagaatt gttgagccac actgtcaggc cctccctgga cacaggaacc
     3601 ggtgcggggg ccgttatctt tataagctgg cgtgacggtg gtacaggcac tcagcagcag
     3661 tgcacatgga ataagtagag atagtgaacg gtagcgcata atcatttcct tattagcttg
     3721 ctgtctgcgt cacactacga ccaaacgcta tcagattatt taatacattg tttttaatac
     3781 aattctgaaa caatctgaac acctgattgg gtttgagtcc attgctggcg acccactcaa
     3841 ggttgctcgt agctgactta cttaaattac ccaaacaata gacagtgtaa accatttgtg
     3901 aggttgagcg tgttatatgg ctgaaatgat catttaaggt aaatatgata gttctgaaat
     3961 ggtggtataa atcctcctgt agaggttaag agcatctgca agaagccagt ttcacattta
     4021 taacgcgtga taaatgaatg cggttattgt ctaatcatta caaaattttt cacatgcgat
     4081 aagagatcct gcgattaaga tgattgccaa aattacgttg tatttttgaa aatgagcgaa
     4141 atttgatagt cccagctttg actctcgata atattattta tgctacaatt gtgggtaaag
     4201 aatttaagct aatgaatttg agtgaagaat aaattaaatt gcttcagtat ttcgttaatt
     4261 aatataaata caaaaggcgt tgtgctttta taattataaa gcgcgaggag ttcggtggat
     4321 tatcgttttt cttgggtgat ttttaatatg ataaacaata tatctgatca agcatcatca
     4381 tttccaggca cacaactaaa tcaaagtgat aatttcctgg attcactgag ggaatttttc
     4441 gcgatattaa atcccagtcg taagggtgag ttgtctacat gggataccat ctacttgcat
     4501 ctgatattag caataaatgc tgacagtgat ctcataaaaa atgatgtgtt gctcgcggaa
     4561 aatattccat cagcgaatta ccaatttaat acatttttta gcaa
//
LOCUS       DAZXUK010000273         4591 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZXUK010000273 DAZXUK010000000
VERSION     DAZXUK010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.273"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(130..1458)
                     /gene="dsdA"
                     /locus_tag="ACH3L8_003642"
     CDS             complement(130..1458)
                     /gene="dsdA"
                     /locus_tag="ACH3L8_003642"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFT4086579.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(1476..1694)
                     /locus_tag="ACH3L8_003643"
                     /pseudo
     CDS             complement(1476..1694)
                     /locus_tag="ACH3L8_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            1925..2920
                     /locus_tag="ACH3L8_003644"
     CDS             1925..2920
                     /locus_tag="ACH3L8_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFT4086580.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            complement(2928..4361)
                     /gene="cscA"
                     /locus_tag="ACH3L8_003645"
     CDS             complement(2928..4361)
                     /gene="cscA"
                     /locus_tag="ACH3L8_003645"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFT4086581.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
BASE COUNT         1122 a         1141 c         1136 g         1192 t
ORIGIN      
        1 tacagcgaaa ggcgttgggt gatgataaaa ggagggggtt atagcgggta agaccgggga
       61 tcttaagtct gcgccatcgg cagcatcgct cacccaggga aaggattgcg atgttgcgtt
      121 gaaacgttat taacggcctt ttgccagata ttgattcatc tcttcttccg gcaccattcc
      181 acctcccgtc gcccacacca gatgagtggc attacgcaat tgttctgcgc tgaaaccgtg
      241 catctgttgg taacttactg atgcacacac gcgctgaggt ccggccatac ccgccagtgc
      301 tgaaggttca agacgaatac cttcttcctg cgccagccag ccaagcatgt catacatggt
      361 ttgatcgcta agggtataga agccatccag cagacgctcc attgcccgcc cgacaaagca
      421 tgatgcgcga ccaactgcaa ggccatccgc tgcggtaagg ttgtcgatac caatatcctg
      481 aacagaaatc tgatcgtgta atcctgtatg gacgcctaac aacatacaag gggagtgcgt
      541 tggttcggca aaaaagcagt gaacatgatc gccaaacgcc agtttaagcc cgaatgcgac
      601 gccaccagga ccaccgccaa caccacacgg cagatagaca aacagagggt tatcagcatc
      661 gacgatacgg ccttgctggg caaattgcgc tttaagacgc tggccagcaa cggaataccc
      721 aaggaacaac gtgcgggaat tttcgtcatc aataaagaaa cagttcgggt cagactgcgc
      781 tgctttacgt ccttcctcga cggcaacacc ataatcttgc tcatattcca cgaccgtaac
      841 gccatggctg cgcagtttcg cttttttcca tgcccgggca tcagcagaca tatgaactgt
      901 caccttaaag ccaatgcggg cgctcataat gccgattgat aaccccagat ttccggttga
      961 gcccacagca atgctgtatt ggctaaagaa ctgtttaaac tccggagaaa gcagtttgct
     1021 gtagtcatca tcaagcgtca gcaaccccgc ttccagagcc agtttttctg catgtgccag
     1081 gacttcataa atcccgccgc gtgcttttat agagccggaa atgggcaaat ggctatcttt
     1141 tttcagtagg agttgcccgc tgatcggttg ttgatattcc ttttccagcc gtttttgcat
     1201 agccggaatg gcaaccagtt ctgattcaat aatcccccca gtggcagctg tttcagcaaa
     1261 tgcttttgcc agatagggtg caaaacggga taagcgcgca tgggcgtcct gaacatcctg
     1321 ttcggtcagg ccaacataag gtaaaccttc agccaatgag gtcgtgctag gattaaacca
     1381 ggtggtttct ttaagagcaa ccagatcctt taccaacgga tactgggcga tgagcgagtt
     1441 cattttagcg ttttccatag tacgtctctt tgcgcttaga tgataaatga cagcaggaat
     1501 gtgcccgcca gagcgacgac tgaagcgata aatgtcgctg tcgtatagta tttaaatgtt
     1561 tcattgagcg tagcgccgca atattgcttc accagccaga aaagcgagtc cgtaacgatc
     1621 gtgcagccaa ttgcacctga accgatagca atcgcaataa tttccgggct gatgtcggga
     1681 tacagtggca gcacaatgat ccatatttat attttcttct ccaggttggc gatgccagtt
     1741 ggcatgtttt acttacttgc ctatatggtt actcgttggc gagaacagag gcggaattgg
     1801 ggacaattgt ccgggaatta ttgttatttt ttattgtatt gctgtgtcag gtgatagggc
     1861 gttggatctc gccagtgacg tctgtttctg ctacagtgcc cgttttacgg caaacggctt
     1921 gggtatggct tcattaaagg atgtcgcacg cctggcggga gtgtcgatga tgacagtctc
     1981 ccgggtgatg cataatgcag aatctgtgcg tcctgcaacg cgtgaccgcg tattgcaggc
     2041 aatccagacc ctgaattatg ttcctgatct ttccgcccgt aagatgcgcg ctcaaggacg
     2101 taagccgtcg actctcgccg tgctggcgca ggacacggct accactcctt tctctgttga
     2161 tattctgctt gccattgagc aaaccgccag cgagttcggc tggaatagtt ttttaatcaa
     2221 tattttttct gaagatgacg ctgcccgcgc ggcacgtcag ctgcttgccc accgtccgga
     2281 tggcattatc tatactacaa tggggctgcg acatatcacg ctgcctgagt ctctgtatgg
     2341 tgaaaatatt gtattggcga actgtgtggc ggatgaccca gcgttaccca gttatatccc
     2401 tgatgattac actgcacaat atgaatcaac acagcatttg ctcgcggcgg gctatcgtca
     2461 accattatgc ttctggctac cggaaagtgc gttggcaaca gggtatcgtc ggcagggatt
     2521 tgagcaggct tggcgtgatg ctggacgaga tctggctgag gtgaaacaat ttcacatggc
     2581 aacaggtgat gatcactaca ccgatctcgc aagtttactc aatgctcact tcaaatcggg
     2641 caaaccagat tttgatgttc tgatatgtgg taacgatcgc gcagcctttg tggcttatca
     2701 ggttcttttg gcgaaggggg tacgtatccc gcaggatgtc gctgtaatgg gctttgataa
     2761 tctggttggc gtcgggcatc tgtttttacc gccgctgacc acaattcagc ttccacatga
     2821 cattatcggg cgggaagctg cattgcatat tattgaaggt cgtgaagggg gaagagtgac
     2881 gcggatccct tgcccgctgt tgatccgttg ttccacctga tattatgtta acccagtagc
     2941 cagagtgctc catgttgcag cacagccact ccgtgggagg cataaagcga cagttcccgt
     3001 tcttctggct gcggatagat tcgactactc atcaccgctt ccccgtcgtt aataaatact
     3061 tccacggatg atgtatcgat aaatatcctt agggcgagcg tgtcacgctg cgggagggga
     3121 atactacggt agccgtctaa attctcgtgt gggtaatacc gccacaaaat aagtcgctca
     3181 gattggttat caatatacag ccgcattcca gtgccgagct gtaatccgta atgttcggca
     3241 tcactgttct tcagcgccca ctgcaactga atctcaactg cttgcgcgtt ttcctgcaaa
     3301 acatatttat tgctgattct gcggggagag acagattgat gctgctggcg taacgactca
     3361 gcttcgtgaa ccgggcgttg tagaagtttg ccattgctct ctgatagctc gcgcgccagc
     3421 gtcatgcagc ctgcccatcc ttcacgtttt gagggcattg gcgattccca catatccatc
     3481 cagccgataa caatacgccg accatccttc gctaaaaagc tttgtggtgc ataaaagtca
     3541 tgcccgttat caagttcagt aaaatgcccg gattgtgcaa aaagtcgtcc tggcgaccac
     3601 attccgggta ttacgccact ttgaaagcga tttcggtaac tgtatccctc ggcattcatt
     3661 ccctgcgggg aaaacatcag ataatgctga tcgccaaggc tgaaaaagtc cggacattcc
     3721 cacatatagc tttcacccgc atcagcgtgg gccagtacgc gatcgaaggc ccattcacgc
     3781 aatgaactgc cgcgataaag caggatctgc cccgtgttgc ctggatcttt cgccccgact
     3841 accatccacc atgtgtcggc ttcacgccac actttaggat cgcggaagtg catgattcct
     3901 tctggtggag tgaggatcac accctgtttc tcgaaatgaa taccatcccg actggtagcc
     3961 agacattgta cttcgcgaat tgcatcgtca ttacctgcac catcgagcca gacgtgtccg
     4021 gtgtagataa gtgagaggac accattgtca tcgacagcac tacctgaaaa acacccgtct
     4081 ttgtcattat cgtctcctag cgctagcgca ataggctcat gctgccagtg gatcatatcg
     4141 tcactggtgg catgtcccca gtgcattggt ccccagtgtt cgctcatcgg gtgatgttga
     4201 taaaacgcgt gataacgatc gttaaaccag atcaggccgt ttggatcgtt catccatccg
     4261 gcaggaggcg cgaggtgaaa atggggatag aaagtgttac cccggtgctc atgaagtttt
     4321 gctagtgcgt tttgcgccgc atgcaatcga gattgcgtca ttttaatcat cctggttaag
     4381 caaatttggt gaattattaa cgttaacttt tataaaaata tagtccctta ctttcataaa
     4441 tgcgatgaat atcgcaaatg ttaacgttaa ctatggcgtt ttgtgatcga atatgcatgt
     4501 tttagtaaat ccatgacgat tttgcgagaa agaggtttat cactatgcgt aactcagatg
     4561 aatttaaggg aaaaaaatgt cagccaaagt a
//
LOCUS       DAZXUK010000274         4585 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZXUK010000274 DAZXUK010000000
VERSION     DAZXUK010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.274"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1507
                     /gene="mltF"
                     /locus_tag="ACH3L8_003646"
     CDS             <1..1507
                     /gene="mltF"
                     /locus_tag="ACH3L8_003646"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFT4086582.1"
                     /translation="LAVALWPSIPWFGKADNRIAAIQARGELRVSTIHTPLTYNEING
                     KPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNY
                     QPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDLQTLKETKFPELSW
                     KVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDEQPVTWFSPL
                     DGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDAVLPQLKP
                     LFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDRTDAEQ
                     SISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPDSWA
                     DVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEAAM
                     QLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN"
     gene            complement(1504..2007)
                     /gene="tadA"
                     /locus_tag="ACH3L8_003647"
     CDS             complement(1504..2007)
                     /gene="tadA"
                     /locus_tag="ACH3L8_003647"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFT4086583.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(2065..2700)
                     /gene="yfhb"
                     /locus_tag="ACH3L8_003648"
     CDS             complement(2065..2700)
                     /gene="yfhb"
                     /locus_tag="ACH3L8_003648"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFT4086584.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2909..3757
                     /locus_tag="ACH3L8_003649"
     CDS             2909..3757
                     /locus_tag="ACH3L8_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFT4086585.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(4096..4585)
                     /locus_tag="ACH3L8_003650"
                     /pseudo
     CDS             complement(4096..4585)
                     /locus_tag="ACH3L8_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1180 a         1201 c         1114 g         1090 t
ORIGIN      
        1 gctcgcggtc gctctctggc catccattcc ctggtttggt aaagccgaca accgtatcgc
       61 cgccattcaa gcgcggggag agttgcgtgt gagcaccatt catactcccc tgacttataa
      121 cgaaatcaac gggaaacctt ttggcctgga ttacgaactg gcgaaacagt ttgccgatta
      181 cctcggcgta aaactgaaag tgaccgtgcg gcagaatatc agccagctgt ttgacgacct
      241 tgataatggt aacgccgacc tgctggcggc aggactggtc tataacagtg agcgggtaaa
      301 aaattatcag cctggcccta cctattattc cgtgtcacaa caactggttt ataaagtggg
      361 tcagtatcgc ccacgtacgc tgggcaacct gacggcggag caactcaccg ttgcaccggg
      421 tcatgtggta gttaacgatc tccagaccct gaaagaaaca aaattcccgg aattaagctg
      481 gaaggtagac gacaaaaaag gctctgcgga attaatggaa gatgtcatcg aaggaaaact
      541 cgattacacc attgctgatt ctgtcgccat cagcctgttt cagcgcgttc acccggagct
      601 cgccgtagcg ctcgatatca ccgatgaaca accggtgact tggtttagcc cgttagatgg
      661 cgataatacc ctttccgccg ccctgctcga cttcttcaac gaaatgaatg aagacggtac
      721 gctggcacgc attgaagaga aatacctggg gcatggcgat gattttgatt acgtcgatac
      781 gcgcacattt ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc tgtttgagaa
      841 atacgccgaa gaaattgact ggcgtttgct ggccgctatt gcttatcagg aatcgcactg
      901 ggatgcacag gccacttcac cgacgggtgt gcgcggcatg atgatgttaa ccaaaaatac
      961 cgcgcaaagc ctcggcatta cggatcgtac cgatgccgaa cagagcatca gcggtggcgt
     1021 gcgttatttg caggatatga tgagtaaagt gccggaaagt gtgccggaga acgaacggat
     1081 ctggtttgcc ctcgctgcgt acaatatggg ctatgcgcat atgctggatg cccgcgccct
     1141 gacggcaaaa accaaaggga atcctgacag ttgggctgac gtaaaacagc gtctgccttt
     1201 acttagccag aaaccctatt acagcaagct gacttacggc tacgctcgtg ggcatgaagc
     1261 ctacgcttat gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt atctgcaaga
     1321 gaaagagaag caggctacag aagcggcgat gcaactggcg caggattatc cggcggtatc
     1381 gcctacggag ttgggcaaag agaaatttcc ttttctctcg tttctttccc agtcgtcatc
     1441 aaactatttg acccattctc cctctctgct gttttccagg aaagggagtg aagagaaaca
     1501 aaattaatcc gtcgaggatt gcgctttttt ctgcgcttta atttcctggc ggcgcatgcg
     1561 aaagaagtca ctgagcaacg ccgcgcactc atccgccagt attccttccg taatttccac
     1621 tcggtgattc atacccggat gatgcagcac atccattaaa gatcccgcag cgccagtttt
     1681 cgcgtcacgc gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg ctccggcaca
     1741 cattacacat ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat aattttgcat
     1801 caccagacca ccctgccgca gggccatgat ttctgcatgt gcggtgggat catggcgacc
     1861 aatcgggcgg ttccagcctt cgccgattac ccgattgtta tgcactaata ccgcgccgac
     1921 cggcacttcc cgctcatccc aggcacgttt cgccagcgtc agcgcgtgac gcatccaata
     1981 ttcgtggcta aattcgactt cagacaaaaa gaaaactccg gttataaaag cgcggcgcat
     2041 tatacacgga cgctatgctt tactctattc cagttgctgg agttcaccgc gcggggttac
     2101 tcgccaacga tgctgacaga aataaagcag cggattgtcc tgattactgt cgctatagcc
     2161 actgtacagc cgcagcggcg tgccgatttt gcgctccagt tgtgcgacct tttcatgtcc
     2221 cagacaacgc atcgtcaata cccaaccacc atagccacgc tgaatttggc tggcgataag
     2281 attaacccgc ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg gctgcggaga
     2341 gccggtaatc aaccagatat cagcatcgga acttaacagg taggtggtta atcgctcctg
     2401 aaccagcgga aaggcggtaa cattgtcgcg aaaccagcgc acgaaatcgg cctgcaacgt
     2461 ctgtaaacgt gcttcgctgt gaccaaaagt gcacccccac agaagcagac tcatcggcca
     2521 gcgtgccgca cgacctttta tcaataacgc aatggctata atcggtaaca acggcaggac
     2581 aagtaacgca ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc cgaacatatc
     2641 ctgctgatgc aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt gagttgccaa
     2701 accatactcc tctggactga aacatcctga ttaattttcg ctactacata gcctaacaga
     2761 tagatcatca cttttccggc aacaatcgac cgcatcatgg ctaactggaa tttttaattc
     2821 atggcaatta gcggcaatgg aatataaaat tcactcgcgt gtgtctcata ttgcctgttg
     2881 tcgccactat ttcatcgcga ggaaagatat gaacggctta cttcgtatcc gtcagcgtta
     2941 ccaagggctt gcccagagtg ataaaaaact ggcggattat ctgctgctac aacctgatac
     3001 ggcgcgccat ttaagctctc agcaactggc caacgaagcc ggagtcagtc agtccagcgt
     3061 cgtgaagttc gcgcaaaaac tcggctataa aggttttccg gcgcttaagt tggcgttgag
     3121 tgaagcgctg gcaagccagc cggaatcgcc ctccgtgccc attcataacc aaatccgcgg
     3181 tgatgatccg ttacggctgg tcggcgaaaa actgattaaa gaaaataccg ccgctatgta
     3241 cgcaacgcta aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa tgttgcgctc
     3301 tgcgcggcgg ataattctga ccggtattgg cgcttcgggt ctggtggcgc aaaactttgc
     3361 ctggaagctg atgaagattg gcttcaatgc tgccgcactg cgcgatatgc atgcgctact
     3421 cgcaacagta caggcgtcgt cccctgacga tctgttatta gccatttcct acaccggtgt
     3481 acgacgcgag ttaaacctgg cggcagatga gatgctgcga gtgggcggaa aagtgctggc
     3541 gattaccggc tttactccga atgccctgca acagcgtgct tctcattgcc tgtataccat
     3601 tgccgaagaa caagcgacaa acagtgcttc aatctctgct tgtcacgctc agggaatgtt
     3661 aacggatttg ctgttcattg cgctgattca gcaggatctg gaactggcac cagaacgtat
     3721 tcgtcatagt gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc gtataatgcc
     3781 cgcccggttt gtgttgtttt gagagtttcc ttatggcgct gttaatcact aaaaaatgca
     3841 tcaattgtga tatgtgtgaa atcaaataaa aacacattta ttttcatagt gatacagcaa
     3901 tgtgttattg aatgtgttgt tggatttatg gtgcaaaaaa attttgcata agttcagcaa
     3961 cacaaagaac accactttct ttaacgtcct ccgcgcccta aaggacgggg ctttacggcg
     4021 caaaaggcct gtgacataca acattaactt ttcagtcaac cgtagcacgt tcttgtatac
     4081 gccgtgtctg cggttttata taaccctggc tggaaacctc ttatacaaag tcgattcgcc
     4141 aacatcatag atgatcgcca ccttctggcg agaaactcct gatgcaatta gtcgcccggc
     4201 ctgcgcccat tattctggtg aaagtttggg acggcgaccg ccaattcgtc cctgtgcgcg
     4261 agcagcttcc agtcctgctt ttgttcgttc aacaatcagt tcacgttcca tttcagccag
     4321 ggcacccatc acatggaaga aaaagcgtcc cattggtgtg ctggtatcaa ttgaatcagt
     4381 cagactacga aagttgatgc ctcgttcgcg caacgcctcc accagcacga caagatgccg
     4441 catactgcgc cccagccgat ccagcttcca gacaaccagt gtgtcgcctg ccgataatgt
     4501 cttgagcagt tttttcagcc ccggtctttc ggactttgta ccacttatct tgtcttcaaa
     4561 aatcagctcg catcctgcac agttc
//
LOCUS       DAZXUK010000275         4575 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZXUK010000275 DAZXUK010000000
VERSION     DAZXUK010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.275"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(346..421)
                     /locus_tag="ACH3L8_003651"
     tRNA            complement(346..421)
                     /locus_tag="ACH3L8_003651"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(386..388),aa:Phe,seq:gaa)
     gene            complement(528..1103)
                     /locus_tag="ACH3L8_003652"
     CDS             complement(528..1103)
                     /locus_tag="ACH3L8_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFT4086586.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(1140..2837)
                     /gene="dsbD"
                     /locus_tag="ACH3L8_003653"
     CDS             complement(1140..2837)
                     /gene="dsbD"
                     /locus_tag="ACH3L8_003653"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFT4086587.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(2813..3151)
                     /gene="cutA"
                     /locus_tag="ACH3L8_003654"
     CDS             complement(2813..3151)
                     /gene="cutA"
                     /locus_tag="ACH3L8_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFT4086588.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(3267..4568)
                     /gene="dcuA"
                     /locus_tag="ACH3L8_003655"
     CDS             complement(3267..4568)
                     /gene="dcuA"
                     /locus_tag="ACH3L8_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFT4086589.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
BASE COUNT         1154 a         1173 c         1226 g         1022 t
ORIGIN      
        1 attgttagtc tttgcatgag caagcggact gacacatttc tctttgttct cattcagaaa
       61 atctcatcag tcggcgttca gaccggtgtt gtgctttatg gatacgttac gctcctgatg
      121 acgtcatttg acgttcaaca gcatcacggg gccgcacgac atttcacgtc agttagtgct
      181 atagctcagg aacaaatttt cccaaattgg gatatgctcg caaattgctg gtgatgtggg
      241 gagaatctgg ttgagttcgg tagaattgat ttggaggcag aacgcttaaa tcgtggcgtc
      301 ctgaaacgaa aaacggacct ccgtggaggt ccgttgatat gaatttggtg cccggactcg
      361 gaatcgaacc aaggacacgg ggattttcaa tcccctgctc taccgactga gctatccggg
      421 caacggggcg cattaaaccg taatctgcac atctcgtcaa cctaatttca gtaaaagcga
      481 ttcaactgct taagattgcg gcaaatcgct tcttttctgt gtttctgtca ggtcaatgca
      541 ccaccctggc ggcagcgggc gaagcgcagg atatcttccg ccagccgatg tgcagtgtcg
      601 acatccgcct ggctacgatt caccagcatt cggcttaaac agccttccag caccagttcc
      661 atctgctttg ctaccatcgc cggatcgtca acttccagcg tggttaacag ttcgtgggtg
      721 aaatcgtagg cagcgctttt ttgctgatcg gccagttgat gaatagggtg gccaggatcg
      781 ggataaaacg tacaggcagc gataaataga cagcccggat agcggttgtt tttaacgcac
      841 tccgataacg cctgataacg tgccagcagc ttttgttcgg cggtttgcgt ttcgtccagc
      901 atcagctgac gacgccagac atctatctgt tggctaagat aacgcagcgc atcgtagagg
      961 attgcctctt tgtctggcca gaagcggcgt agctcgtcca gtggataatc cacacgttca
     1021 gcaaccatct ccagcgtggt gttggcaatc ccttgtaatt ctaataattt cagggcttct
     1081 cccagtacat cttcacgttg cacgctattt tcctccgtct ttcccactgc aagtgtcgtt
     1141 cacggttggc gatcgcgcaa atgtgcgctg aaggtttcag catccataaa gcccgtgacg
     1201 cgtgcttgtg gatgctcctg gccttgtccg tcaaaaaaga gaattgtcgg taggccaagg
     1261 acattaagat gctttaacag cgccacatct tgtgcgtcgt tggccgtgac gttggcctga
     1321 agtaagaccg tgtctgctaa cgctttttgc acctgcgggt cgctgaaggt gtatttctca
     1381 aactctttac aggcgacgca ccagtcggca taaagatcta acatcaccgg tttgcctttg
     1441 gcttcaacga gcgcctgatt taactcatct accgttttga tttgtgtaaa gttgagatgc
     1501 gtttgagttt gcgcggtatg cgtcgcacca aatgcccaat cctgaagtgg gcgcacgcta
     1561 accaatgccg ctgccagcag gattatttgc accacgcgca tccagccgcg tttagcctgt
     1621 aggctggtga taaaggccca gccaaagaat gcgacaccca gcgccgacca caagcgtaat
     1681 ccccatacat caccaatcac tcgctccagc aggaagaccg gcagtgcgag gatcacaaaa
     1741 ccaaacgcgg ttttgacttg ttccatccac gggccgcttt tcggcagcaa gcggttacca
     1801 aagacggtaa ttagcatcag cggcaggccc atgcccaacg catagagata aagcgtgccg
     1861 ccgcccggcc acatgttccc gctttgggcg atatacagca gaatcgcgct aagcggtgcg
     1921 gtggtgcatg gtgaacagat cagtccggca atcgccccca taacaaacac accgctaggt
     1981 gagccgccct gttggcgatt gctcatcaac gtgagacgtg tttgcagcga agaggggagt
     2041 tgtagagtaa acaagccaaa cattgacatc gccagcaagg taaagacgat ggcgaggcca
     2101 atgagcacgt atgggtgctg tagcgccgcc tggaactgta accctgcggc ggcaactacc
     2161 agacccagcg ccgtgtaagt cagtgccatc ccctgcacat aaataaaggt cagcaacaat
     2221 gctctggcag tggagagccg ctgtttacca cccagcacga tgccagaaat cagtgggtac
     2281 attggcagca cgcatggcgt aaaggcgata ccaataccga tcaacaacgc ccagagcgcg
     2341 gaaaagggca attgcgcggt gtgctgctct tgctgcgaaa cagacacagg ctgtgacgct
     2401 gcgttgttgg cgaccacttc gcttaacgga acggttttgg tttctggcgg ataacagaaa
     2461 ccggcatcag cacagccctg gtaggtgaca gttaacgtcg ctcccgcact cgcctggttg
     2521 atggttacgg gaagcgtcag ccgatcgcgg taaatctcgc ttttgccgta aaactcatct
     2581 tcatgccaga cgccttgcgg cagctgctcg tcggcaattt tcgcgtgttc cggcgtaatg
     2641 cggatctgtt tacggtagag gtagtaaccg tctttgatct gccaggtcag attaaggtca
     2701 tgttggtttt gctgaaaatc aaaagcaaag gcttgatccg cggggacaaa ttgtgaacgt
     2761 cccggcgcgt cgaataatcc ggcaaaaacg gaagtgctgc aaagtagcag aatcagcgta
     2821 aagatgcgtt gagccatgag aggtaatctg tgtctccgtg tgtaacaggt aaaaccagaa
     2881 gttccggggt ttgatatgga tgatgagact tcaggcattc cagcagtgcc tgctggtgag
     2941 atacggtagt ttttaaaatc atctgcactt cgtattcttg ctccagctta ccttcccagt
     3001 aatagagaga ggtagcgccg gggatcaagg tcgcgcaggc cgccagtttt tccgccagca
     3061 ctttggcggc taaatcctgg gctgtcgctt catccggtgc ggtacatagc accacgacag
     3121 acgtggtatt cgaacttttt tcatcaagca taaacacctc gcaagaacag atggaaccgc
     3181 aagagagagg tcactataca acggacgggg gaaggatgtt agtcggcggg aaagaaagcg
     3241 gggcgtgaac gccccgcgat tgatcattac agcatgaagc tacccagcac gaagccgaag
     3301 caaacggcca gggcaacacc cagagtaccc gggatgaaga acggatggtt gaagacgaat
     3361 ttaccgatac gggtagtacc cgtgtcatcc atctgtaccg cagcaaccag cgtcgggtag
     3421 gtcggcagaa tgaacagacc agacaccgca gcgaaagaag caacagcggt cagcggtgaa
     3481 acgttcagtg ccagagccat cggcatcagt gcttttgcgg ttgcagcctg agagtacagc
     3541 agagcagaag caaagaagaa gatgacggcc agcagccacg gatgaccctg aatcacttca
     3601 ccagcggtat ctttgatcca gtcgatgttg ttggaaacga aagtatcgcc cagccacgca
     3661 acacccagga tacaaataca ggcgctcata cctgctttga aggtgctgga gttgaggatg
     3721 ttgtcggtat cgactttaca gataacggtg gtcagagttg caacgctgag catgatgatc
     3781 aggattgcgt tggtggtgtt catcagcggt ttttcaacca gacccatgct tgggctgttg
     3841 atgattgcat agataaccac gccaactacg cccagcagga acagccagac ggacgttttt
     3901 gcaccggatt tgatttcaat ctgcttttca ccgcgcagtt caaccaagcc ctcttccaga
     3961 cgcttgcgat aaatcggatc gtcagagagt ttggagttga acagcatagt gaccaggaag
     4021 gacatcacca gaaccgccag cagggtggac gggatgacca cggagagcag atggaggtag
     4081 ctgatgccat gaccttccat cacggaagac atgtaaacca ctgccgctga gattggcgat
     4141 gcggtgatcg caatctgcgc ggataccact gcagtagaga gcggacggca aggtttaacg
     4201 ccttgttcct tcgcaacttc agcgataact ggcagtgtcg ccagagagat gttgccagta
     4261 ccagcaaaga tagtcaggaa ataggtcacg atcggtgcga ggatcgtgat gtatttcggg
     4321 ttacggcgca gcagcttttc tgtctgatga accagatagt ccagaccgcc agcaacctgc
     4381 atggcagaaa tagcggcgat aaccgccatg ataatggaaa tgacatcgaa cgggatgtta
     4441 ccgggtttaa cgccaatagc ggcaagaacc agcaccccca atccgcctgc aaaaccaata
     4501 cctattcccc ccaatctggc gcccaagaag atcgccagca aaactatgat gagctctaca
     4561 actagcatat tagcc
//
LOCUS       DAZXUK010000276         4543 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZXUK010000276 DAZXUK010000000
VERSION     DAZXUK010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.276"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..851
                     /locus_tag="ACH3L8_003656"
     CDS             <1..851
                     /locus_tag="ACH3L8_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFT4086590.1"
                     /translation="RHIIIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGFN
                     ILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEI
                     VARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEI
                     YESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNP
                     LNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGT
                     IYPIEK"
     gene            873..2282
                     /locus_tag="ACH3L8_003657"
     CDS             873..2282
                     /locus_tag="ACH3L8_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4086591.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="ACH3L8_003658"
     CDS             2260..3039
                     /locus_tag="ACH3L8_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFT4086592.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="ACH3L8_003659"
     CDS             3036..3896
                     /locus_tag="ACH3L8_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFT4086593.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4543)
                     /locus_tag="ACH3L8_003660"
                     /pseudo
     CDS             complement(4044..4543)
                     /locus_tag="ACH3L8_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
BASE COUNT         1039 a         1125 c         1270 g         1109 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggagtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggcggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggcag ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cga
//
LOCUS       DAZXUK010000277         4511 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZXUK010000277 DAZXUK010000000
VERSION     DAZXUK010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.277"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            143..2383
                     /gene="fepA"
                     /locus_tag="ACH3L8_003661"
     CDS             143..2383
                     /gene="fepA"
                     /locus_tag="ACH3L8_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFT4086594.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            2558..3178
                     /gene="entD"
                     /locus_tag="ACH3L8_003662"
     CDS             2558..3178
                     /gene="entD"
                     /locus_tag="ACH3L8_003662"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFT4086595.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(3300..3452)
                     /gene="hokE"
                     /locus_tag="ACH3L8_003663"
     CDS             complement(3300..3452)
                     /gene="hokE"
                     /locus_tag="ACH3L8_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFT4086596.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(3803..3955)
                     /gene="hokE"
                     /locus_tag="ACH3L8_003664"
     CDS             complement(3803..3955)
                     /gene="hokE"
                     /locus_tag="ACH3L8_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFT4086597.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            complement(4052..>4511)
                     /locus_tag="ACH3L8_003665"
     CDS             complement(4052..>4511)
                     /locus_tag="ACH3L8_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFT4086598.1"
                     /translation="FAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFK
                     KLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSE
                     CFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
BASE COUNT         1198 a         1139 c         1146 g         1028 t
ORIGIN      
        1 ccgtgggaag cgcggacatt aattaaccaa ctgcactgcg tgtctttcag gatcaaaggt
       61 tttcgcggta acgggatgcg tcgtgttgat gacgaccatg cccgacagtt gcaattcgtg
      121 gcaaaaatgc aggaataaaa caatgaacaa gaagattcat tccctggcct tgttggtcaa
      181 tctggggatt tatggggtag cgcaggcaca agagccgacc gatacacctg tttcacatga
      241 cgataccatt gtcgttaccg ccgccgagca gaacttgcag gctcctggcg tttcgaccat
      301 caccgcagat gaaatccgca aaaacccggt tgcccgcgat gtgtcggaga tcatccgtac
      361 catgccaggc gttaacctga ccggtaactc caccagtggt cagcgtggga ataaccgaca
      421 gattgatatt cgcggtatgg gtccggaaaa cacgctgatt ttgattgacg gcaagccggt
      481 aagcagccgt aactcggtgc gtcagggctg gcgtggcgag cgcgataccc gtggtgatac
      541 ctcctgggtg ccacctgaaa tgattgaacg tattgaagtt ctgcgtggtc cggcagctgc
      601 gcgttatggc aacggcgcgg cgggcggcgt ggttaacatc attaccaaaa aaggcagcgg
      661 cgagtggcac ggctcctggg acgcatattt caatgcgcca gaacataaag aggaaggtgc
      721 caccaaacgc actaacttta gcctgaccgg tccgctgggc gacgaattca gcttccgctt
      781 gtatggcaac ctcgacaaaa ctcaggctga cgcgtgggat atcaaccagg gccatcagtc
      841 cgcgcgtgcc ggaacgtatg ccacgacgtt accagccggg cgcgaagggg tgatcaataa
      901 agatattaac ggcgtggtgc gctgggactt cgcaccattg caatcgctgg aactggaagc
      961 gggttacagc cgccagggta acctgtatgc gggcgatacc cagaacacca actctgacgc
     1021 ttacactcgc tcgaaatatg gcgatgaaac caaccgcctg tatcgccaga actacgcgct
     1081 gacctggaac ggtggctggg ataacggcgt gaccaccagc aactgggtgc agtatgaaca
     1141 cacccgtaac tcgcgtattc cggaaggtct ggcgggcggt accgaaggga aatttaacga
     1201 aaaagcgaca caggatttcg tcgatatcga tctcgatgac gtgatgctgc acagcgaagt
     1261 taacctgccg attgatttcc tcgttaacca gacgctgacg ctgggtacgg agtggaatca
     1321 gcaacggatg aaggacttaa gttccaacac ccaagcactg accggaacga ataccggtgg
     1381 cgctattgat ggcgtgagtg ccaccgaccg tagcccgtat tcaaaagcag aaattttctc
     1441 gctgtttgcc gaaaacaaca tggagctgac tgacagcacc atcgtaacgc cggggctgcg
     1501 tttcgatcat cacagtattg tcggcaataa ctggagcccg gcgctgaaca tatcgcaagg
     1561 tttaggcgat gacttcacgc tgaaaatagg cattgcccgc gcctataaag cgccgagcct
     1621 ataccagact aacccgaact acattctcta cagtaaaggt cagggctgct atgccagcgc
     1681 gggcggctgc tatctgcaag gtaacgatga cctgaaagta gaaaccagca tcaacaaaga
     1741 gattggtctg gagttcaaac gcgacggctg gctggcgggc gtcacctggt tccgtaacga
     1801 ttatcgcaat aagattgaag cgggctatgt ggctgtaggg caaaacgcag tcggcactga
     1861 tctctatcag tgggataacg tgccgaaagc agtggttgaa ggtctggaag gatcgttaaa
     1921 cgtaccagtt agcgaaacgg tgatgtggac caataacatc acttatatgc tgaagagtga
     1981 aaacaaaacc acgggcgacc gtttgtcgat catcccggag tacacgttga actcaacgct
     2041 gagctggcag gcacgggaag atttgtcgat gcaaacgacc ttcacctggt acggcaagca
     2101 gcagccgaag aagtacaact ataaaggtca gccagcggtt ggcccggaaa ccaaagaaat
     2161 cagtccttac agcattgtgg gtctgagcgc gacctgggat gtgacgaaga atgtcagtct
     2221 gaccggcggc gtggacaacc tgttcgacaa acgtttgtgg cgtgcaggta atgcccagac
     2281 cacgggcgat ctggcaggag ccaactatat cgccggtgcc ggcgcgtata cctataacga
     2341 gccgggacgt acgtggtata tgagcattaa tactcacttc tgatgctaac gtcagattgt
     2401 tgacaaagtg cgcgtcgttc acgccggatg cggcgtgaac gccttatccg gcctacaaag
     2461 atcttgccaa ttcaatatat tgcaggatca cgtaggcctg ataagcgtag cgcatcaggc
     2521 agttttgcgt ttgtcatcag tctcgaatat ggtcgatatg aaaactacgc atacctccct
     2581 cccctttgcc ggacatacgc tgcattttgt tgagttcgat ccggcgaatt tttgtgagca
     2641 ggatttactc tggctgccgc actacgcaca actgcaacac gctggacgta aacgtaaaac
     2701 agagcattta gccggacgga tcgctgctgt ttatgccttg cgggaatatg gctataaatg
     2761 tgtgcccgca atcggcgagc tacgccaacc ggtgtggcct gcggaggtat acggcagtat
     2821 tagccactgc ggggctacgg cattggccgt ggtttcccgt caaccgattg gcgttgatat
     2881 cgaagaaatt ttttctgcgc aaacagcgac cgaattgaca gacaacatta ttacaccagc
     2941 agaacacgag cgactcgcag actgcggttt agccttttct ctggcgctga cacttgcatt
     3001 ttccgccaaa gagagcgcat ttaaggcaag tgagatccaa actgatgcag gttttctgga
     3061 ctatcagata attagctgga ataaacagca ggtcatcatt catcgtgaga atgagatgtt
     3121 tgctgtgcac tggcagataa aagaaaaaat agtcataact ctgtgccaac acgattaatt
     3181 gacaacatct ggtacgattc gcccgcagcc atcactgact gcgggcgaaa gtgtaaagca
     3241 ggtgccttac cactctgacc tgacaaccgg atatgcgggg attactcccc gcacaacggc
     3301 tacttcttcg gttcgtaagc aagaacagcc ttaaactcaa tattacgttc ctttacggtg
     3361 aactcacaca gcgagtctcc gaccagaagc gtaaatccca gcaccgtcaa acacagcact
     3421 atgactgcca caagggcata tttcgtcagc atgtttatat tgcctccatg cgaagaaggg
     3481 actaccatcc gagttgttga ggttcggagt ggcagcccca ggattgatag aaatatctct
     3541 tggggcttca tctttccaaa cctcagagtt aacctgaaac cggaaagctt caggcacccg
     3601 ccgttatctt acccttcctt agcaaaatag ctgtttattt atacagtgag tcctgtttcg
     3661 gacttctgtc ggcaaaactc atcattgctc ttaaaacgaa atcgcccgca gccatcactg
     3721 accgcgggcg aaagtgtaaa gcaggtgcct taccatcctg acctgacaac cggatatgcg
     3781 gggattactc cccgcacaac ggctacttct tcggttcgta agcgagaaca gccctgaact
     3841 caatattacg ttccttcacc gtaaactcac acagcgagtc tccggccaga agcgtaaatc
     3901 ccagtaccgt caaacacagc actatgactg ccacaagggc atatttcgtc agcatgttta
     3961 tattacctcc atgcgaagaa gggactacca tccaaattgt ctggtttgga gtggcagccc
     4021 cagggtgtac tgaccccaaa aagttggaca gttatacacg aggcatatag gtctgattcc
     4081 gatattcaat tggagtcaga ccttttaatt tcaggctaat tcttctgctg ttatagtatt
     4141 caatatattc cgtaacagca tccttcagtt cgcttatatt actgaactca tcaagataaa
     4201 aacactccga ctttaaggtt ccaaagaaac actccaccac agcattatcc agacaattgc
     4261 cttttctgga catgctttgt ttaataccat gttctttaag gatattttga tatcttctca
     4321 tacgatactg ccatccctgg tcagagtgca gaacaggatg ctcgtgagga ttaagctttt
     4381 tgaatgcctg atcgagcata ttctcaacca tgttcatcac tggtctttcc gaaaggctgt
     4441 aagaaataac ttcgttgttg aagagatcta ttactggaga caaatacagc ttgcgcccat
     4501 tgactgcaaa t
//
LOCUS       DAZXUK010000278         4508 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZXUK010000278 DAZXUK010000000
VERSION     DAZXUK010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.278"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            155..2587
                     /gene="hsdR"
                     /locus_tag="ACH3L8_003666"
     CDS             155..2587
                     /gene="hsdR"
                     /locus_tag="ACH3L8_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFT4086599.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2654..4123
                     /locus_tag="ACH3L8_003667"
     CDS             2654..4123
                     /locus_tag="ACH3L8_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFT4086600.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4123..>4508
                     /locus_tag="ACH3L8_003668"
     CDS             4123..>4508
                     /locus_tag="ACH3L8_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFT4086601.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSE"
BASE COUNT         1299 a         1126 c         1104 g          979 t
ORIGIN      
        1 ctctgatatg cccagattaa ttcaagcaag atataacaga gatatcaatg tgatctctgc
       61 tacgtacttt acgttgaaac accgccctaa atcatagcca ggctattgat tttatgtaca
      121 atcagaatcg tcctgataaa agcgagaacc ccccatggcg gaactgaacc taagtaacct
      181 gacggaagca gacatcatta ccaaatgcgt tatgccagcc attctcaatg caggctggga
      241 caacacaacg caaatcagac aggaggtcaa actccgggac ggtaaagtca tcgtacgtgg
      301 taaagttgcg gcacgcagaa cggtaaaatc tgctgacatc gtgctgtacc acaagccagg
      361 cattccctta gccgtgattg aagcaaaagc caacaaacat gaaattggca aagggatgca
      421 acagggcatt gaatatgcgc gcctgctgga cgttcctttt gtttttgcca ccaacggtga
      481 tggctttatc ttccgcgatg ccaccgcagc cgaaggtgaa tgccttgaaa agcaaataac
      541 gctggatgac ttcccctccc ccgctgaact ctggcaaaaa ttctgcctct ggaaaggata
      601 cacacaagct cagcttccgg tgattactca ggactattac gacgatggca gcggcaaatc
      661 gcctcgttat tatcaacttc aggcaatcaa caaaaccatt gaagccgtct ccaacgggca
      721 aaaccgcgtt ctgctggtca tggcgaccgg aacagggaaa acctataccg cattccagat
      781 catctggcgc ctgtggaaat caaaaaataa aaaacgcatt ttgttccttg ccgatcgcaa
      841 cattctggtc gaccaaacca aaaataatga tttccagcca tttggtacgg caatgaccaa
      901 agtcagcgga cgcaccattg atcccgctta tgaaattcac ctcgcgctct atcaggctat
      961 aactggcccg gaggaagacc aaaaagcgtt taaacaagtc gcaccagatt tcttcgatct
     1021 gatcgtgatc gacgaatgcc atcgcggcag cgcatccgaa gacagcgcct ggcgagagat
     1081 ccttgattat ttcagttccg ccacccaaat tggcttaacc gccacgccaa aagagacgca
     1141 tgaagtctcc agcacggatt acttcggcga tccggtttac gtctactcac taaaagaagg
     1201 gatcgaagac ggcttcctcg ctccttataa agttgtccgt gttgatattg atgtcgacct
     1261 gcaaggctgg cgcccaacca aagggcaaac tgacttaaac ggcgaagtga ttgacgatcg
     1321 tatctataac cagaaagatt tcgatcgcac gatggtgatc gacgaacgca cagaactggt
     1381 tgccagaacc attaccgact acctcaagcg caccaatccg atggataaaa ccatcgtctt
     1441 ctgtaacgac atcgatcatg cagaacgtat gcgccgcgcc ctggttaatc tcaacccgca
     1501 gcaggtgaaa aagaacgaca aatacgtcat gaaaatcacc ggcgatgatg aaattggcaa
     1561 agctcagttg gataacttca tcaacccgaa aaaaccgtac ccggttatcg cgaccacttc
     1621 agagctgatg accactggtg tggatgctaa aacctgcaag ctggtagtac tagaccagaa
     1681 catccagtcg atgaccaaat tcaagcaaat tatcggtcgt ggtacacgca tcgacgaacg
     1741 ttacggcaaa ctctggttta ccatcctcga ctttaaaaaa gccaccgaac tgtttgccga
     1801 tgagcgtttc gatggcattc ccgaaaaagt catggatacc acaccagagg atatcgccga
     1861 tccagaatct gattttgaag agaaactcga agaaatcagc gaacatgacg acgaacaggt
     1921 aacaggcgtt gatgaaccgc ctgcgccacc ataccaggtt accgataccg atgatgtcgg
     1981 cccatttccg gaagaagacg agaagaaaat ccgcaagttt cacgtcaacg gtgtagcggt
     2041 gggcgttatt gcacagcgcg ttcagtatta cgacgccgac ggtaaactgg ttaccgaatc
     2101 ctttaaagat tacacccgca aaacactgct caaagaatat gcctcgctgg atgactttac
     2161 ccgcaagtgg caggacgccg atcgcaaaga agcgatcatt cacgagctgg agcaacaggg
     2221 gatcatctgg gaagtactgg cagaagaagt cggtaaagat ctcgacccgt tcgacatgct
     2281 ttgccacgta gtgtatggtc agccgccgtt aacccgcaaa gagcgcgccg agaacgtgca
     2341 caagcggaac tacttcacaa aatactctga agcagcgcaa gccgtgctcg ataatctgct
     2401 ggataaatac gccgatgcgg gcgtacagga gatcgaaagt attcaggtgc tgaaacttaa
     2461 accattcgac agcatgggca ccttaccgga gattattaaa accggatttg gcgaccgtaa
     2521 cgggtataat caggcgctca gcgagctgga aaacgaaatc taccaattac cgccccgctc
     2581 tgcttaacaa ctctcttttg cgccttctgc aaccatcatg ttgcagaagg ccttgttttt
     2641 catggaatac aacatgtcta tcagctcagt aatcaaatca ttacaagata ttatgcgcaa
     2701 agatgccggt gtggacggcg atgcgcagcg tctcggtcag ctctcctggc tgctgttttt
     2761 gaaaatcttc gatgcccagg aagaggcgct ggaactggag caggataact atcaatatcc
     2821 gatcccacag cgttatttgt ggcgcagttg ggccgccaac gcgcagggca ttaccggtga
     2881 ttccctgctg gaattcgtta atgatgatct gtttccggcg ttgaaaaacc tcactgcgcc
     2941 tatcgataaa aacccacgcg gctacgtggt gaagcaggcg ttcagcgatg cctataacta
     3001 tatgaaaaac ggtacgctac tgcgccaggt gatcaacaag ctgaacgaaa ttgactttac
     3061 cagcgccagc gaacgccatc tgtttggtga tatttacgaa cagatcctta aagatctgca
     3121 atctgcgggc aatgcgggcg aattctatac tccacgcgcc gtcactcgct ttatggtgga
     3181 tcgcgttgat ccgaaactcg gcgaatccat tatggacccg gcctgcggta cgggcggttt
     3241 tcttgcctgc gcgttcgatc atgtaaagaa caaatacgtg aagagcgtcg ccgatcatca
     3301 gacactgcaa caacagatcc acggtgttga gaaaaaacag cttccgcacc tgctggcgac
     3361 caccaatatg ctgctgcacg gcattgaagt accggtacaa attcgtcacg acaacaccct
     3421 gaataaaccg ctttcctcct gggatgagca actggatgtc attgttacca acccgccgtt
     3481 tggtggcacg gaagaagacg gtattgagaa gaactttccg gcagagatgc aaacccgcga
     3541 aacggcggat ctgtttctgc aactgattgt ggaagtactg gcgaaaaacg gtcgtgcggc
     3601 ggtggtattg ccggatggca cactatttgg cgaaggtgtt aaaaccaaaa tcaaaaagct
     3661 acttaccgaa gagtgcaacc tgcataccat cgtgcgttta ccgaatggtg tgtttaatcc
     3721 ctataccggc attaaaacca acctgttgtt ctttaccaaa ggtcagccaa ccaaagagat
     3781 ttggttctat gaacatccgt atccggcggg cgtgaaaaac tacagcaaaa ccaagccgat
     3841 gaagtttgaa gagtttcagg cggagatcga ctggtggggt aacgaggccg atggttttgc
     3901 cagccgcgtc gagaatgagc aagcgtggaa agtcagcatt gatgacgtga ttgcgcgcaa
     3961 cttcaatctg gatattaaaa acccacatca ggcggaaacc gtcagccatg atccggacga
     4021 actgttagcg cagtatgcaa aacagcaggc ggagatccag acgctgcgta atcaactgcg
     4081 cgatattctt ggcgctgcgc tgtctggcaa ggaggttaac taatgagtgt ggaaaagctg
     4141 atcgttgatc atatggaaac ctggacctcg gcgttgcaaa cccgttccac cgccgggcgc
     4201 ggcagctccg gtaagattga tttgtatggt attaagaaat tgcgtgagct gattctggaa
     4261 ctggcggtgc gcggtaaact ggtgccgcag gatccgaatg atgaaccggc gtcggacctg
     4321 ctgaagcgta ttgcggcgga aaaagcagag ctggtgaagc aaggcaaaat taaaaagcaa
     4381 aaaccactgc cagaaattag tgaggaagag aagccgtttg aattgccgga gggatgggag
     4441 tgggtgcacc tccctgatat ttattgctca atttctgagt catctagaaa aataaaatcc
     4501 tccgaaat
//
LOCUS       DAZXUK010000279         4500 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZXUK010000279 DAZXUK010000000
VERSION     DAZXUK010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.279"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..147)
                     /locus_tag="ACH3L8_003669"
     CDS             complement(<1..147)
                     /locus_tag="ACH3L8_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFT4086602.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENE"
     gene            451..1383
                     /gene="rfaD"
                     /locus_tag="ACH3L8_003670"
     CDS             451..1383
                     /gene="rfaD"
                     /locus_tag="ACH3L8_003670"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFT4086603.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1393..2439
                     /gene="rfaF"
                     /locus_tag="ACH3L8_003671"
     CDS             1393..2439
                     /gene="rfaF"
                     /locus_tag="ACH3L8_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFT4086604.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2443..3435
                     /gene="rfaC"
                     /locus_tag="ACH3L8_003672"
     CDS             2443..3435
                     /gene="rfaC"
                     /locus_tag="ACH3L8_003672"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFT4086605.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3432..>4500
                     /gene="rfaL"
                     /locus_tag="ACH3L8_003673"
     CDS             3432..>4500
                     /gene="rfaL"
                     /locus_tag="ACH3L8_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFT4086606.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNK"
BASE COUNT         1213 a         1009 c         1079 g         1199 t
ORIGIN      
        1 ctcattttca agtgcggcca gacgctcaaa gtaactaaaa taaacatcaa ctgaacgagc
       61 tagtttttca cggaaccctt tattagcagt ccaatcccct ctaccaatgt caaaatatgc
      121 agtgataatt gtggtggatg atttcattac taataagacc attaatataa aaaaagaatt
      181 atatcataaa tattaacaga atgtatactg tatattatcc attcaaatga ttccgttatt
      241 tatcatacat cggaatattg atactaaagc actattcaca tgcaaaacca acatccgcca
      301 tgaaggacta gctaaaaccc aaactagttt gttgcaatta gcatccttgc acctctatgt
      361 aaagggctga agggattcgg atgtgatggt atgattacag acattcgtgt ctgagattgt
      421 ctctgactcc ataattcgaa ggttacagtt atgatcatcg ttaccggcgg cgcgggcttt
      481 atcggcagca acatcgttaa ggccctgaat gataaaggca tcaccgatat tctggtggtg
      541 gacaacctga aagacggcac caagtttgtg aacctggtgg atctggatat cgcggactat
      601 atggataagg aagacttcct gatccagatt atggctggcg aagagttcgg tgatgtcgaa
      661 gcgattttcc acgaaggcgc atgctcttcc accaccgagt gggatggcaa gtatatgatg
      721 gataacaact atcaatactc caaagagctg ctgcactact gtctggagcg cgaaatcccg
      781 ttcctgtatg cctcttccgc agccacctac ggcggacgca cctccgactt tattgaatcc
      841 cgcgagtacg aaaaaccgtt gaatgtctac ggttactcaa aattcctgtt tgatgaatat
      901 gttcgtcaaa tcctgccaga agcgaactcg cagattgttg gcttccgcta tttcaacgtt
      961 tatggaccgc gtgaaggcca taaaggcagc atggcgagcg tcgctttcca tctcaacacc
     1021 cagcttaata acggtgaatc accgaagctg ttcgaaggta gcgagaactt caaacgtgac
     1081 ttcgtctatg tgggcgacgt ggcagatgta aacctgtggt tcctggaaaa tggcgtttcc
     1141 ggtatcttca acctcggcac cggtcgtgca gaatccttcc aggcagtagc agatgctacg
     1201 ctggcttatc acaagaaagg ccagatcgaa tacattccgt tcccggataa gctgaaaggc
     1261 cgctaccagg cgttcactca ggcagatctg acaaatctgc gcgcggcggg ttacgacaaa
     1321 ccgttcaaaa ccgttgctga aggtgtaacg gaatacatgg cctggctgaa tcgcgacgca
     1381 taagagctct gcatgaaaat actggtgatc ggcccgtctt gggttggcga catgatgatg
     1441 tcgcaaagtc tctatcgcac gctccaggcg cgctatcccc aggcgataat cgatgtgatg
     1501 gcaccggcat ggtgccgtcc attattatcg cggatgccgg aagttaacga agctatccct
     1561 atgcctctcg gtcacggagc gctggaaatc ggcgaacgcc gcaaactggg tcatagcctg
     1621 cgtgaaaagc gctacgaccg cgcctacgtc ttaccaaact ccttcaaatc tgcattagtg
     1681 cctttcttcg cgggtattcc tcatcgcacc ggctggcgcg gcgagatgcg ctacggttta
     1741 ctcaacgatg tacgcgtgct cgataaagaa gcctggccgc taatggtgga acgctatgtc
     1801 gcgctggcct atgacaaagg cattatgcgt accgcacaag atctgccgca gccattgtta
     1861 tggccgcagt tgcaggtgag cgaaggtgaa aaatcatata cctgtaatca attttcgctt
     1921 tcatcagaac gtccgatgat tggcttttgc ccgggtgcgg agtttggtcc ggcaaaacgc
     1981 tggccacact accactatgc ggaactggca aagcagctga ttgatgaagg ttatcaggtg
     2041 gttctgtttg gctctgcgaa agatcatgaa gcgggcaatg agattcttgc cgctttgaat
     2101 accgagcagc aggcatggtg tcggaacctg gcgggggaaa cacagcttga tcaagcggtt
     2161 atcttgattg cagcctgtaa agccattgtc actaacgatt ctggcctgat gcacgttgcg
     2221 gcggcgctca atcgtccgct ggttgccctg tatggtccaa gtagcccgga cttcacaccg
     2281 ccgctatccc ataaagcgcg cgtgatccgt ctgattaccg gctatcacaa agtgcgtaaa
     2341 ggtgacgctg cggagggtta tcaccagagc ttgatcgaca ttactcccca gcgcgtactg
     2401 gaagaactca acgcgctatt gttacaagag gaagcctgac ggatgcgggt tttgatcgtt
     2461 aaaacatcgt cgatgggcga tgttctccat acgttgcccg cactcactga tgcccagcag
     2521 gcaatcccag ggattaagtt tgactgggtg gtggaagaag ggttcgcaca gattccttcc
     2581 tggcacgctg ccgttgagcg agttattcct gtggcaatac gtcgctggcg taaagcctgg
     2641 ttctcggccc ccataaaagc tgaacgcaaa gcgtttcgtg aagcgctaca agcagagaac
     2701 tatgacgcag ttatcgacgc tcaggggctg gtaaaaagcg cggcactggt gacacgtctg
     2761 gcgcatggcg taaagcatgg attggactgg caaaccgctc gcgaaccttt agccagcctg
     2821 ttttacaatt gtaagcatca tattgcaaaa cagcagcacg ccgtagaacg cacccgcgaa
     2881 ctgtttgcca aaagtttggg ctatagcaaa ccgcaaaccc agggcgatta tgctatcgca
     2941 cagcattttc tgacgaacct gcctacagat gctggcgaat atgccgtatt tcttcatgcg
     3001 acgacccgtg atgataaaca ctggccggaa gaacactggc gagaattgat tggtttactg
     3061 gctgattcag gaatacggat taaacttccg tggggcgcgc cgcatgagga agaacgggcg
     3121 aaacgactgg cggaaggatt tgcttatgtt gaagtattgc cgaagatgag tctggaaggc
     3181 gttgcccgcg tgctggccgg ggctaaattt gtagtgtcgg tggatacggg gttaagccat
     3241 ttaacggcgg cactggatag acccaatatc acggtttatg gaccaaccga tccgggatta
     3301 attggtgggt atgggaagaa tcaagtagag tgtcgttcta caagtatgtc tcttgcagat
     3361 ttgccagctc aaaccgtttt tcaaaattta aatcttgaaa taataaccaa taagttgaca
     3421 tcggagataa gatgacctca acattatttt tctctctcga gaaaaaaaac tggatagcgt
     3481 actggaacag agctctcgta ttcttattca ttaccaccta ttttttgggt gggattacaa
     3541 ggtataaaca tcttattgtt attcttatga caataacgac aatcgtctat ctctgcaaac
     3601 ggccaaaaca ctatctctca ctatttaaaa catttctttt tggtagtgtt gccatattaa
     3661 ctattgctgc attgctgtca cttcttcaat cccctgatgc aggtgctagc atgaaggaag
     3721 ttttcaaagc tattattgag aatactttac tatgcacaat agcaataccg gtcatattga
     3781 gagacgagaa aagagaagat gtcgaaaaaa tcgttttctt ctcatttatt agtgcgttgg
     3841 gcttacgctg tttttctgaa ttgattacct attataagga ctatcaacaa gggataatgc
     3901 cattcgcaga ttatagacac cgtagcattt ctgactcgat ggtcttttta ttccctgcat
     3961 tgttaaatct ctggcttatc aaatcagcaa aataccgcat ttcttttgtg gttctaagcg
     4021 ttatttttat ttttctgata ttaggaactt tatccagagg ggcctggctt tccgtgttag
     4081 tcattggatt aatatggatt ctgatgttta aacaatggaa gttactatta gtaggagtaa
     4141 tggtttccat cattgcattg tcggttattt tcacacataa ggagatgacc gcaaagctaa
     4201 cgtataaact tcaacaaact aatagttctt atcgctatgc aaatggtact caaggcagcg
     4261 cactcgatct aatattagaa aatcctgtta ttggttatgg ttacggtaac gttgcatata
     4321 aagatgtcta taataaacgt gtcattgatt atccagaatg gacctttaga caatcaatag
     4381 ggccacataa ttttgcgcta ttcatctggt ttggcactgg tttattaggg ctggtaagtc
     4441 ttatgatgct atactgtgca atattgaaag agtgtataaa aaatggcgtc aagaataaat
//
LOCUS       DAZXUK010000280         4446 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZXUK010000280 DAZXUK010000000
VERSION     DAZXUK010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.280"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            161..1018
                     /gene="focA"
                     /locus_tag="ACH3L8_003674"
     CDS             161..1018
                     /gene="focA"
                     /locus_tag="ACH3L8_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFT4086607.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1073..3355
                     /gene="pflB"
                     /locus_tag="ACH3L8_003675"
     CDS             1073..3355
                     /gene="pflB"
                     /locus_tag="ACH3L8_003675"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFT4086608.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3547..4287
                     /gene="pflA"
                     /locus_tag="ACH3L8_003676"
     CDS             3547..4287
                     /gene="pflA"
                     /locus_tag="ACH3L8_003676"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFT4086609.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4369..>4446)
                     /locus_tag="ACH3L8_003677"
     CDS             complement(4369..>4446)
                     /locus_tag="ACH3L8_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086610.1"
                     /translation="FDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT         1130 a         1101 c         1080 g         1135 t
ORIGIN      
        1 aatttatttt tatttggata atcaaatagt tactccgtat ttgcataaaa accatgcgag
       61 ttacgggcct ataagccagg cgagatatga tctatatcaa tttctcatct ataatgcttt
      121 gttagtatct cgtcgccgac ttaataaaga gagagttagt gtgaaagctg acaacccttt
      181 tgatctttta cttcctgctg caatggccaa agtggccgaa gaggcgggtg tctataaagc
      241 aacgaaacat ccgcttaaga ctttctatct ggcgattacc gccggtgttt tcatctcaat
      301 cgcattcgtc ttctatatca cagcaaccac tggcacaggc acaatgccct tcggcatggc
      361 aaaactggtt ggcggcattt gcttctctct ggggctgatt ctttgtgttg tctgcggagc
      421 cgatctcttt acttccacag tgttgattgt tgttgctaag gcgagtgggc gcatcacctg
      481 gtgtcagttg gctaaaaact ggctaaatgt ctattttggc aacctggtcg gcgcactgct
      541 gtttgtactt ttaatgtggc tttccggcga gtatatgacc gcaaatggtc aatggggact
      601 aaacgtccta caaaccgccg accacaaagt gcaccatact tttattgagg ccgtctgtct
      661 tggtatcctg gcaaacctga tggtatgtct ggcagtatgg atgagttatt ctggccgcag
      721 cctgatggac aaagcgttca ttatggtgct gccggtcgcg atgtttgttg ccagcggttt
      781 tgagcacagt atcgcaaaca tgtttatgat cccgatgggt attgtaatcc gcgacttcgc
      841 atctccggaa ttctggaccg ctgtcggttc tgcaccggaa aatttttctc acctgaccgt
      901 gatgaacttc atcactgata acctgatccc ggttacgatc ggtaatatta tcggcggtgg
      961 tttgttggtt gggttgacat actgggtcat ttacctgcgt gaaaacgatc accattaatg
     1021 gttgtcgaag tacgcagtaa ataaaaaatc cacttaagaa ggtaggtgtt acatgtccga
     1081 gcttaatgaa aagttagcca cagcctggga aggttttacc aaaggtgact ggcagaatga
     1141 agtaaacgtc cgtgacttca ttcagaaaaa ctacactccg tacgagggtg acgagtcctt
     1201 cctggctggc gctactgaag cgaccaccac cctgtgggac aaagtaatgg aaggtgttaa
     1261 actggaaaac cgcactcacg cgccagttga ttttgacacc gctgttgctt ccaccatcac
     1321 ctctcacgac gctggctaca tcaataaagc gttggaaaaa gttgttggtc tgcagactga
     1381 agctccgctg aaacgtgctc ttatcccgtt cggtggtatc aaaatgatcg aaggttcctg
     1441 caaagcgtac aaccgcgaac tggacccgat gatcaaaaaa atcttcactg aataccgtaa
     1501 aactcacaac cagggcgtgt tcgacgttta cactccggac atcctgcgtt gccgtaaatc
     1561 cggtgttctg accggtctgc cagatgctta tggccgtggc cgtatcatcg gtgactaccg
     1621 tcgcgttgcg ctgtacggta tcgactacct gatgaaagac aaatacgctc agttcacctc
     1681 tctacaggct gatctggaaa acggcgtaaa cctggaacag actatccgtc tgcgcgaaga
     1741 aatcgctgaa cagcaccgcg ctctgggtca gatgaaagaa atggctgcga aatacggcta
     1801 cgacatctct ggtccggcta ccaacgctca ggaagctatc cagtggactt acttcggcta
     1861 cctggctgct gttaagtctc agaacggtgc tgcaatgtcc ttcggtcgta cctccacctt
     1921 cctggatgtg tacatcgaac gtgacctgaa agctggcaag atcaccgaac aagaagcgca
     1981 ggaaatggtt gaccacctgg tcatgaaact gcgtatggtt cgcttcctgc gtactccgga
     2041 atacgatgaa ctgttctctg gcgacccaat ctgggcaacc gaatctatcg gtggtatggg
     2101 cctcgatggt cgtaccctgg ttaccaaaaa cagcttccgt ttcctgaaca ccctgtacac
     2161 tatggggccg tctccggaac cgaacatgac cattctgtgg tctgaaaaac tgccgctgaa
     2221 cttcaagaaa ttcgccgcta aagtgtccat cgacacctct tctctgcagt atgagaacga
     2281 tgacctgatg cgtccggact tcaacaacga tgactacgct atcgcttgct gcgtaagccc
     2341 gatgatcgtt ggtaaacaaa tgcagttctt cggtgcgcgt gcaaacctgg cgaaaaccat
     2401 gctgtacgca atcaacggcg gcgttgacga aaaactgaaa atgcaggttg gtccgaagtc
     2461 tgaaccgatc aaaggcgatc tcctgagcta cgatgaagtg atggagcgca tggatcactt
     2521 catggactgg ctggctaaac agtacatcac tgcactgaac atcatccact acatgcacga
     2581 caagtacagc tacgaagcct ctctgatggc gctgcacgac cgtgacgtta tccgcaccat
     2641 ggcgtgtggt atcgctggtc tgtccgttgc tgctgactcc ctgtctgcaa tcaaatatgc
     2701 gaaagttaaa ccgattcgtg acgaagacgg tctggctatc gacttcgaaa tcgaaggcga
     2761 atacccgcag tttggtaaca atgatccgcg tgtagatgac ctggctgttg acctggtaga
     2821 acgtttcatg aagaaaattc agaaactgca cacctaccgt gacgctatcc cgactcagtc
     2881 tgttctgacc atcacttcta acgttgtgta tggtaagaaa actggtaaca ccccagacgg
     2941 tcgtcgtgct ggcgcgccgt tcggaccggg tgctaacccg atgcacggtc gtgaccagaa
     3001 aggtgctgta gcgtctctga cttccgttgc taaactgccg tttgcttacg ctaaagatgg
     3061 tatctcctac accttctcta tcgttccgaa cgcactgggt aaagacgacg aagttcgtaa
     3121 gaccaacctg gctggtctga tggatggtta cttccaccac gaagcatcca tcgaaggtgg
     3181 tcagcacctg aacgttaacg tgatgaaccg tgaaatgctg ctcgacgcga tggaaaaccc
     3241 ggaaaaatat ccgcagctga ccatccgtgt atctggctac gcagtacgtt tcaactcgct
     3301 gactaaagaa cagcagcagg acgttattac tcgtaccttc actcaatcta tgtaattaga
     3361 tttgactgaa atcgtacagt aaaaagcgta caataaaggc tccacgaaag tggggccttt
     3421 tttagcacga gagccttttt tgtcagctat ctatacttta aggtgactgc caaaacagac
     3481 tcgacgtagc cttcgagctg cgcaccaaca cggcctcaga tgggccacat ctggagaaac
     3541 accgcaatgt cagttattgg tcgcattcac tcctttgaat cctgtggaac cgtagacggc
     3601 ccaggtattc gctttatcac ctttttccag ggctgcctga tgcgctgcct gtattgtcat
     3661 aaccgcgaca cctgggacac gcatggcggt aaagaagtta ccgttgaaga tttgatgaag
     3721 gaagtggtga cctatcgcca ctttatgaac gcttccggcg gcggcgttac cgcatccggc
     3781 ggtgaagcaa tcctgcaagc tgagtttgtt cgtgactggt tccgcgcctg taaaaaagaa
     3841 ggcattcata cctgtctgga taccaacggt tttgttcgtc gttacgatcc ggtgattgat
     3901 gaactgctgg aagtaaccga cctggtaatg ctcgatctca aacagatgaa cgacgagatc
     3961 caccaaaatc tggttggagt ttccaaccac cgcacgctgg agttcgctaa atatctggcg
     4021 aacaaaaatg tgaaggtgtg gatccgctac gttgttgtcc caggctggtc tgacgatgac
     4081 gattcggcac atcgcctcgg tgaatttacc cgtgatatgg gtaacgttga gaaaatcgaa
     4141 cttctcccct accacgaact gggcaaacac aaatgggtgg cgatgggtga agaatacaaa
     4201 ctcgatggtg ttaaaccacc gaagaaagag accatggaac gcgtgaaagg cattcttgag
     4261 cagtacggtc ataaggtaat gttctaattt cctcgtaaag cggcaacttt attgagttgc
     4321 cgctttttta ttccgcgctt cattctgcta cagcgttttt aacacatctt atagtgcatc
     4381 taccatatcc cgtacctgag ataataacga ctgataatag ctcgcgtgaa gttcttcccc
     4441 atcgaa
//
LOCUS       DAZXUK010000281         4441 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZXUK010000281 DAZXUK010000000
VERSION     DAZXUK010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.281"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..377
                     /locus_tag="ACH3L8_003678"
     CDS             <1..377
                     /locus_tag="ACH3L8_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFT4086611.1"
                     /translation="TVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINP
                     DSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQ
                     GYLYQKPMPFSAWDKSGKLVKE"
     gene            385..1713
                     /locus_tag="ACH3L8_003679"
     CDS             385..1713
                     /locus_tag="ACH3L8_003679"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFT4086612.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(1760..3085)
                     /gene="rimO"
                     /locus_tag="ACH3L8_003680"
     CDS             complement(1760..3085)
                     /gene="rimO"
                     /locus_tag="ACH3L8_003680"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFT4086613.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            3298..3681
                     /gene="bssR"
                     /locus_tag="ACH3L8_003681"
     CDS             3298..3681
                     /gene="bssR"
                     /locus_tag="ACH3L8_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFT4086614.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3792..>4441
                     /gene="yliI"
                     /locus_tag="ACH3L8_003682"
     CDS             3792..>4441
                     /gene="yliI"
                     /locus_tag="ACH3L8_003682"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFT4086615.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNP"
BASE COUNT         1121 a         1008 c         1116 g         1196 t
ORIGIN      
        1 aaaccgtgat cgagcgaatt aatgaacatt tttatctcaa tattgaacaa ctgcgtaaac
       61 aaggggtacg gatatcgatt gatgactttg gcaccggttt gtcaaacctg aaacgttttt
      121 atgaaattaa tccagacagc ataaaggtgg actcgcaatt taccggcgat attttcggta
      181 ctgcgggaaa aattgtgcgc attattttcg atctggcacg ctataaccgg atcccggtga
      241 ttgcggaagg cgtagagagc gaagacgttg cgcgcgaatt aattaaatta ggatgtgttc
      301 aggctcaggg gtatctgtac cagaaaccca tgccattctc cgcttgggat aaaagtggaa
      361 aattagtaaa agagtagttt acgtatgtcc agaatcaata agttcgtact tacagtcagt
      421 ctgctgattt ttatcatgat ttcagcagtt gcctgcggga tctacactca aatggtaaag
      481 gaacgggtgt atagcctgaa acagtccgtt attgatactg cttttgcggt ggcaaatatt
      541 gctgaatatc ggcgtagcgt ggcaattgat cttatcaaca cgctaaatcc cacggaggaa
      601 cagctgttgg ttggtttgcg cacagcttac gccgactcgg tttccccctc ttatttgtac
      661 gatgtcggtc cttatctgat ttccagtgac gaatgtattc aggtaaagga gttcgagaaa
      721 aattattgtg cagatattat gcaggttgtg aagtatcgac atgtcaaaaa tacagggttt
      781 atctcttttg acggtaaaac cttcgtctat tacctctatc cggtaactca caatcgtagt
      841 ctgatatttt tgcttggtct ggagcgtttt tctttactgt caaaatcgct ggtgatggac
      901 agcgagaacc tgatgttctc tctatttaag aacggtaaac cggtgaccgg tgatgaatat
      961 aatgctaaaa acgccatctt caccgtttcg gaagcgatgg agcacttcgc ctatttgccg
     1021 accggattgt acgtatttgc gtataaaaaa gatgtttatt tgcgggtttg tacattgatt
     1081 attttctttg ccgcattggt ggcagtgata ttgggtgcca gttgcctcta tctggtacgc
     1141 agagtgatta atcgtggtat tgtggagaaa gaagccatca ttaataacca ttttgaacgc
     1201 gtactggatg gcgggctttt cttttcggct gccgatgtca aaaaactcta cagtatgtat
     1261 aactcggcgt tcctggacga cctgaccaaa gcaatgggca gaaaatcctt tgatgaagat
     1321 ttaaaagcgc tgccggaaaa aggcggttat ttgtgtctgt ttgacgtcga taaattcaaa
     1381 aacattaacg acaccttcgg tcatttgctg ggcgatgaag tgttgatgaa agtggtgaaa
     1441 atccttaaat cacagatccc ggtagataaa ggtaaagtct accgcttcgg aggtgacgaa
     1501 tttgcggtga tttatactgg cggcacgctg gaagagttgc tatcgatact aaaagaaatc
     1561 gttcatttcc aggtgggaag cattaattta agcaccagta tcggtgtagc gcattcaaat
     1621 gaatgtacta ccgtcgaacg cttgaaaatg ctggcggatg agcggctgta taagagtaag
     1681 aaaaacggca gggcacagat tagctggcag taatcattat tcgcaggccg gacaaatgat
     1741 tttgcccggc ctgaattaat taaacccggc taccccacaa atcgtactca tcggcgtgct
     1801 cgactttcac acgcaggata tcacccggct taacgttggt ttcaccattg agataaaccg
     1861 cgccgtcgat ttccggtgca tctgccatgc tgcgaccaat cgcgccttct tcgtccactt
     1921 cgtcgataat caccagaatt tcacggccca ctttctcttg caggcgctcg gcggaaatct
     1981 gctgctgcag ctgcatgaaa cggttccagc gttcttcttt cacttcttcc ggaacctggt
     2041 caggcagggc attggcgtct gcaccttcaa ccgggctgta tttaaagcag ccaacgcgat
     2101 ccagacgtgc ttctttcagg aagtcgagta gcatctggaa atcttcttcc gtctcgccag
     2161 ggaagccgac aataaaggtt gagcgtaggg tcagttccgg gcagatttcg cgccactgtt
     2221 tgatgcgcgc cagttggcga tctacagaac ccggacgctt catcagtttg agaatgcgcg
     2281 ggctggcgtg ctgcaacgga atgtccagat acggcaggat tttgccttct gccatcagtg
     2341 ggatgacgtc gtccacatgc ggataagggt aaacgtagtg cagacgtgtc cagatcccca
     2401 gtttcgataa ctgttcgcac aggctgacca tgctggtttt taccggctcg ccgttgtgga
     2461 agccagtacg atgtttaaca tcaacgccat aggcggaagt atcctgcgag atcaccagaa
     2521 tctctttaac gcccgcatct accagacgtt tcgcttcact taacacttcg ccaatcggac
     2581 ggctcatcag gtcgccgcgc atagacggaa taatgcagaa ggtgcagcgg tgattacagc
     2641 cttccgaaat tttcagatag gcataatgac gcggcgtcag tttcacacct tgttctggca
     2701 ccaggctcag gaatgggttg tgtttcggtt ttggcacgta gtgatgaacg tgctccagaa
     2761 cctgctcata gctatgaggc ccggtgattt ccagcacttt cgggtggact tcgcggatct
     2821 gatcttcttt tgcccccaga caaccggtca caattacctt gccgttttca ttcaacgctt
     2881 caccaatggc ttccagtgat tcttgtaccg cgctgtcaat aaagccgcag gtgttgacga
     2941 tcaccatgtt cgcatcgtca tagctcggta ccacgtcata accttcagtg cggagttcgg
     3001 tgagaatacg ctctgaatcg acaaggtttt tcggacagcc aagggaaaca aagccgattt
     3061 tcggctgggg agttactttg ctcatagctt aaaaaatatt cagttacagg aaaggtcagg
     3121 gcagggattc tacagagttc tggataaaat ttgtatcgca atctcattcg ctggcggagg
     3181 cgaaggaaat gtaaattttg ttaattcggc gtgaagaatt gatcctggac agcattttgc
     3241 tcaaaaaata gccatactat ttaattgcaa caaggctgga aagaggagga tcgaagtatg
     3301 ttcgttgaca gacagcgaat cgatctgctg aaccggttga tcgacgcacg cgttgacctc
     3361 gctgcatacg tgcaactgag gaaggcaaaa ggatacatgt ccgtcagcga aagcaatcat
     3421 ctacgagata acttttttaa actgaatcgc gaactgcacg ataaatcgct gcggttgaat
     3481 cttcatctgg atcaggaaga gtggagtgct cttcatcatg ctgaagaagc attagcgaca
     3541 gccgcagtat gtttgatgag tgggcaccat gattgcccga ctgttattac cgtcaacgcc
     3601 gataagcttg aaaattgtct gatgagctta acgctgagta tccagagcct gcagaagcac
     3661 gccatgcttg agaaggcctg aaaactaagg gggagaaagc gtctccccct tcatgtttaa
     3721 gtttttgtaa aaatgaattt gttatctcct ccactgacta cgctttaagc cagagtcaat
     3781 ccggaggcgt tatgcatcga caatcctttt tccttgtgcc ccttatttgt ctttcttccg
     3841 ctctctgggc ggctcctgca acggtaaatg tcgaagtact gcaagacaaa ctcgaccatc
     3901 cctgggcact ggccttttta cccgataatc acggtatgtt aatcactctg cgcggcggcg
     3961 agttgcgtca ctggcaagca ggaaaaggat tatctgcgcc gctttccgga gttccggacg
     4021 tttgggcgca cgggcagggc ggcctgctgg acgtggtttt agcgcctgat tttgctcagt
     4081 ctcgccgcat ctggttaagt tattccgaag ttggcgatga tggcaaagcc ggaactgctg
     4141 tgggttatgg ccgcttaagt gatgatctct caaaagtgac cgacttccgc accgtcttcc
     4201 gccagatgcc aaaactgtct accggcaacc attttggcgg gcggctggta ttcgacggta
     4261 aaggttatct ttttattgct ctgggcgaaa acaatcagcg cccgacggcg caggatctgg
     4321 ataaattaca gggcaaactg gtgcgtctga ccgaccaggg cgaaatcccg gatgataatc
     4381 cttttataaa ggaatccggt gcgcgcgccg agatctggtc ttatggcatt cgtaatccgc
     4441 a
//
LOCUS       DAZXUK010000282         4400 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZXUK010000282 DAZXUK010000000
VERSION     DAZXUK010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.282"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..195)
                     /locus_tag="ACH3L8_003683"
     CDS             complement(<1..195)
                     /locus_tag="ACH3L8_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086616.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
     gene            complement(252..2612)
                     /locus_tag="ACH3L8_003684"
                     /pseudo
     CDS             complement(252..2612)
                     /locus_tag="ACH3L8_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(2650..4335)
                     /locus_tag="ACH3L8_003685"
                     /pseudo
     CDS             complement(2650..4335)
                     /locus_tag="ACH3L8_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
BASE COUNT         1199 a          976 c          973 g         1252 t
ORIGIN      
        1 caatgaaccg gtatgaattt gcagccataa atttacctga tcctttggat gagcatgcgg
       61 ataaatcata tatcgcaagt cattgtccag ttgcccggta attaactttt catcctgcgg
      121 taaggcggcg gcaatcagcc gcccggggag caacagagtt gccactaacg tcagtaagaa
      181 acagaggttt ctcataatta tctccatgcg aaaaccgggc gaatttaccc ggttaagtaa
      241 aatccgaact attaaaattt catgctgaca tcaagccaga aagtacggcc actggcgtag
      301 gtcgccacac cggtgttcgt tgtatcaaca gcggttttgc tatccagtac attgagaata
      361 tcggcactga tcgtcaggtt ttgttgtttc agaaattgtg gcgtctagga caagcgggtg
      421 tcccaggtca ggctgctatc cagtttttcg tcaacatact gcttgtaatc tgaatattcg
      481 ctgatgtatt gcgcatttgt cttaccaagg ataatgcgag ctttacgcgc ttcttgccag
      541 gccagcgtat tcgcccacac taacccgctc ggttgatgcg tgaaatccat gtttaaggag
      601 atctttaatg ggttattaaa atctgccact ggaacgctat cgtaagagac cagattaccg
      661 ttataaacca cctggttatc accggtattg ctctcctcat aaccattgtt taacgacaag
      721 ttgcccttgc tcttgatata gctaaagaca atttgcgggt taatatctac ctggcggata
      781 tgcaggggtt cggccagttc aaaactgagg ttaaacgaat gcgttttggt tttgccatcg
      841 ttgttatatt cagtaatggt ggttttagtc gcgctgtcgg tacgactgct tttgctgatt
      901 tgatcatgcg cttcacttgc gcgtgcaata acgttcttat cgattttttg ctgcaatccc
      961 attgccagtt catcgttata aggcgttttc aaattctgat aacgcgtcag ggttttatta
     1021 cctgataccg attccgtcca gctattgcgg atatcacgta atcccatatc aagaatattc
     1081 ccgccatagt aacggttata accggcggta atcattgagg tttgatcagc aaaaatatcc
     1141 cattccgtca taaagcgcgg ggagatattg tggtttgaca gatagttgtc atagtcataa
     1201 cgcacaccgg gcattaacga cacattccgc cagctaatgt gatccgccat atacagcgtg
     1261 tagttgtcaa ttcccaggct gcctttacct ttatggtaaa tggtatggtt agttttcttt
     1321 cctgcagcat taatcacata ggattcagac tggttatggc gttcagtcca tgcatcggaa
     1381 tagatgtatt ccgcgccgaa gtagggttga tgcgaaacat cacccacggc gaatttttgc
     1441 cagtccaggc gtgttttgaa ggtgtaatta tctacagcct gggaaatgtg tcctaatccg
     1501 ccacgggtac aacgccctgt aatatcacca tatgtacatg aaagttcggt gtaccagatg
     1561 tcatgatcgt gacgggtata atcactaata tgatcccaac caacggtggt acgtagtttg
     1621 gcccatgcga gttgcgtatc catatcccat gccagaccat aggatttatt acccatttcg
     1681 cgatcagact gcgggaaggt gctggtatta taatcacggc tggagccggt atattttaag
     1741 gttaaatcgt gggtaaagcg gtcgctggca aaccaggtaa atttgctcaa tgcagtatcg
     1801 ataacgtttt tatactgtgc ccgaccggcg acaatgccgt cattcgaaac ataatccgcg
     1861 cgggtgatat cagactggcg gcgcgataaa ccggcggtaa cgccaaagtt atcagccagc
     1921 tcctgattaa acgacaaggt ataaaagttc tttttaaaat ctggagagaa ataggtactt
     1981 cctgaagaac cttgattaaa tgcgctcttg ttattctcat cgatatgcga tgttaaccag
     2041 tccaaacgcg tagtgcgata acccaatttc accttgctat catcagcgtt gaagcgtttg
     2101 atctttgcat caattacccc gccattgaag cgaccaaatt caaccggcac aaaactgtca
     2161 taaagcgtca cattgtccag taagctgaca tcaagataat acccctgtga catcccgcta
     2221 atattggttg cactactggc atcggactca ttcgctgggt tcaggttatt agttgcacta
     2281 ataccgtcaa tcaaataggc attctggtag ggcgacgcac cgtgaataga gattttctca
     2341 gggcgaatat ctccctggtt caacgaggta ctttgcgttg aatccatgcg taccgcaggg
     2401 ttggttctca gcagatcgct gatattgcca ttcccggtcg gtaaacgctc gatgctttca
     2461 ctggtgtaat gcgtgttgcc gtttacgggt acagggacag gagcatagac cgtcatctct
     2521 tcaaaaaaat acatgtacat gtttttgtca tcgacggcct gcgccacatc agcgccacat
     2581 aaaatgacgc caggaataag aactcgcttc atttatttat atctccgtac agcagggctt
     2641 ttattgttgt tataaaaccg tgctaatgtc acaaatatca tcagccaggt tccagacacc
     2701 gggttgatgt gtaaccataa tgacgccgct tgtgggtagt ttttcacgca ctaaacgcag
     2761 taagcgtata gcctcttgtt cctcaagatg agaggtagtt tcgtcaagaa atatccattt
     2821 cggacgtcgt aaaattaatc gtgccagggc gatacgttgt ttttcgccgc tggaaagaat
     2881 atctccccag cgatcgtggt catgaatacg cgcagccaat tttcccagac caacctgatg
     2941 cagtacttcg ctcaacgatt tatcgtctac gggcagggga agtgctttac aaataatctc
     3001 tttcagtaag ccgcttttga ttaacggtgt ttgtgacaca taccaactgt cagcaggaga
     3061 agaaatatca cctttaaacc acggccagca gtgggataat gttttaagca gtgtggtttt
     3121 tcccgcacca gagtagcctt tcagtaatag ccatttgcct ggcgaaacat gaaagttcaa
     3181 gttctctaat atgatcttat tatcaggcgt acgaatactc gcattagcca cttgtaccgc
     3241 atgttggcaa tttttaggct tattcgtagg gcgctattca gtgagttgat ggaactcata
     3301 caagcgatcg ataaccgcag ccagttcagc aagttcgtca tatttataaa taaaccagct
     3361 taaattgttc gatactagca taaatgcctg gcgcgatttc atcagtccgc ccagattgat
     3421 ctgcccgcta ataaactgcg gcaataacag aaagtaggga agaacgctaa gcgagcgcga
     3481 ataaatattt tgccagtaat caagccaccg ctgacgattc attaaacgat gccagttctc
     3541 tttaatggta tgaaaattgt cgcgcaactc ctggcgttgt agagactcag cattgcttag
     3601 cgcaatcagt tctgcctgct tattatgctg cacaagatta gttcgaaatg tcgcttcgct
     3661 acgttgtttt tccacattaa gcggacgaat acgtttaccc accttatggg taaataaagt
     3721 tccaccgatc acaatgagca cgacggtata gaccatatac ccctggatat tccattctgt
     3781 tccaccaaca gtgaatgaga gcgtacccgc gctttgccaa agaataacgg taaaggtgat
     3841 cagcatgcta agtgactgga tgaagccaaa tgaaagactg agcgttttgt tgatcagtaa
     3901 gagaatgtct tcagcgatac gttggtcagg gttatctgta tttttatgct cgccgtagat
     3961 ctgcgtgaag tagtagtttt tatctgcaaa ccagcgatta aggtagtaat ctgttaacca
     4021 ttcgcgccag cgaatagtca gtagtttaat taaccatgtt ttgtttgcgg atattaatac
     4081 aaagatcccc agcagggcag ggaaccaaag aacgagctgc caaagcttat cggtttcttt
     4141 ctggcttagc gcattgaaaa aatcattatt ccagtcgttt aaccagacct gaattttaac
     4201 cacgccaagg atcattgcga ggataataat gattaacaac actgaagttt tattattctt
     4261 acgcagccaa aagggcttca gcagacagag atactttgct atcaacatac aaaacgtaat
     4321 gggaatggtt atcattagcg aaaattgatt gtgccatcca atgattatct atggcaatga
     4381 tttactgatg aaatttattt
//
LOCUS       DAZXUK010000283         4350 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZXUK010000283 DAZXUK010000000
VERSION     DAZXUK010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.283"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(125..694)
                     /locus_tag="ACH3L8_003686"
     CDS             complement(125..694)
                     /locus_tag="ACH3L8_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFT4086617.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            893..1093
                     /locus_tag="ACH3L8_003687"
     CDS             893..1093
                     /locus_tag="ACH3L8_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086618.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVCILCSW"
     gene            complement(1439..1582)
                     /locus_tag="ACH3L8_003688"
                     /pseudo
     CDS             complement(1439..1582)
                     /locus_tag="ACH3L8_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
     gene            complement(1916..2182)
                     /locus_tag="ACH3L8_003689"
     CDS             complement(1916..2182)
                     /locus_tag="ACH3L8_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086619.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            2323..2529
                     /locus_tag="ACH3L8_003690"
     CDS             2323..2529
                     /locus_tag="ACH3L8_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFT4086620.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            2624..3226
                     /locus_tag="ACH3L8_003691"
     CDS             2624..3226
                     /locus_tag="ACH3L8_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086621.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            complement(3132..3328)
                     /locus_tag="ACH3L8_003692"
                     /pseudo
     CDS             complement(3132..3328)
                     /locus_tag="ACH3L8_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333284.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1024 a         1168 c         1046 g         1112 t
ORIGIN      
        1 cactgtggtt gtcacacaat ggaacactga cgtaaaaaac agtcagtggg cgagtggata
       61 catcgttgcc gccagacaac aacccatact tttggtttgc cggatattgg cgcgccagtc
      121 gtgctcaact ccggctacag ccaaaattgc gctcaccggt gcagtgagct ttggtatgct
      181 ccttcgccag atagtcagca cgttccagca cttgctgaaa gccggtgtca tcattacgcg
      241 ccagccacac cgccggccag gccggaaaat gcaccagcgt ggcatggcac ccgacatcca
      301 ctcccagggc actgcaccac gcctgtttaa tcatcccggc cagagactcc ggagcgcggt
      361 aatcaccggc acgacaccag gtatcccggt tgaccagcag catcaggtga tagtgttttt
      421 tacctttgca ctccccaaac tcccgggccc agacgtaatg cagggttgtg ggatgcacgc
      481 gtttaccttc ccgatgctta cgtttctggt aagcgtcgat tcgggctttc agggcattga
      541 tgaagcggga tatcacagct gcgtccgttg ctgccggtac atccgggaga cgcaggtcaa
      601 cccgcaatgc cgtcaggcgg ggatgaacat tcagtgcgtg ccgcaccgtc tcacgaatac
      661 gttgctgcca gaaggggttg tatttgtagg tcatggttaa atctccgtat ggttcatacg
      721 gaatagccac gtcgtaaaaa atacgcagag tccctgaagt gacctccggc aggacactgt
      781 ctcaggcact ctgtgataac ccgaacggtc gtctccggac tgacggttga attttcagcg
      841 tcgtttttta atgtaaaaaa ccggttacgg gtaaggctgt gaggagggag tgatggtgat
      901 acgcaaaaag aagtgcaggg actgtggaaa cgcgattact cataatacag tgtgttgccc
      961 gtattgtggt tctgtcgacc ccttcggcta ctaccggaat acagaccgta tcgttaccat
     1021 actgctggcg ctgattattg tggtcctgct gactactgtc agcgtcagtg tgtgtatact
     1081 gtgtagctgg tgagataacg gttcatgtat cccctgagct gacagtgtac cgtgtatatt
     1141 aagcgtaacc gacaggttct ccttcatacc atattaaatt ttccagggat tgccgctgcc
     1201 ttatttcgtc ccatacagga aaactcatct gggtttctga caaatacgcg aaactcacag
     1261 cccctcaata tcaaccacac gttgtaagcc ggtaatacct gaaaatcagc ctattaccgc
     1321 ccacgcctcc ggcaccattc ccttttgatt tcgaattctt tgttgtcgct ataccgctta
     1381 atgttcccgg tcctccggtg cagataacac cggagtcatc gtagcgctgc ttcaatattc
     1441 aggtgacgta tttccagacg gaagccggcg cgcgatcgta aagtttgttc attgtcagtt
     1501 ctgccagccg gttgtcaacg gcagagttaa aggcttccag ctccgcaagg gtaagtttat
     1561 aacgcgtgtg ggaattcact tttcccgcgt ctcctgacgt attacgggct gccacttacc
     1621 tggtgtcccg caatacagcc ttagtgttcc gttatcgggg atccggacgc accgcaacaa
     1681 ccggggaagt atttctggtt tagagcagtg tcaagaggga tttctcagcc cctcactcac
     1741 cggctcagtc tcagggcagg agcattaata cgctcagttc atgtcttata caggaatggg
     1801 cacaggggga gctggcgtgt cactgctgat tttacgcatc agcgttcatg tatctgcact
     1861 ctgatacatg aacgattcac agcgctctgt taccggagag atttactctc ttagcttact
     1921 ctggcaaatc ctttacgtta ctctctgatg acttcttata tttataatac caatcagaca
     1981 gccttatccc atcggtgata tcagtctcct cctcgccagg aacgggctga ctcactgtaa
     2041 ctacctccga aaacaactct gcaattatca gaacgcctgc ttctctccct gtcctcacga
     2101 aaactatccc ctctttatcg cgcgtgcgtg cggaagcatc ttttcgcaac aaccacccgg
     2161 tataccggta cggctcagcc accgcaacct ccccgtttat ctcctggcgg tcacattccc
     2221 gattattttt tacgtttctc cccggttgtt atgccggtga aggtggcgcg ctgttttcat
     2281 gaccacaccg gctggtttaa caacatccgg aggaacattc tcatggctac cccagtttca
     2341 ctgatggatg accagatggt cgacatggca tttatcactc aactgaccgg cctgacagat
     2401 aagtggtttt acaggctcat cagggatggg gcctttccgg ctcccatcaa gctgggccgc
     2461 agctcccgct ggctgaaaag tgaagtggaa gcctggctac aggcgcgtat tgcacagccc
     2521 cgcccgtaat ttctgaccgt tatccgttca cccgcagcag acgtaatccc cggcctgccg
     2581 gcggcattct gctgcctgtt atatccccgt gaggaatatg aaaatgaaac aacagtatca
     2641 gacccgctac gaatggctcc acgaaagcta ccagaaatgg ctgaccggct tcacccggca
     2701 cgccgtatcc tggggcgtgt gtcatccgaa tatctactac ttccataatc tgacgcccgg
     2761 ttgggtgtca ttcaatggtg aaaaaccgga gattgccatc gtaccccaga gcctgcaccg
     2821 gctgatttat ggccctgaca aacgggccac gccgcccctg gatgatgatc tgatagtgaa
     2881 tttatgcacc agtgaacatc tgctggttca tcatccgatg ctggaaggca ttctcctgtc
     2941 tgaatgtgaa cgtctgaggc agcgttctct ggcaaataaa ctgatcagcc tcttccgtca
     3001 gtttggtggc acggagttgc gcctcaaact ggtctggctt tgctggcttg atttaatgac
     3061 cggaaacagc cttgaagact ggaaagagaa cctgaaacgg aaatcagaaa aagagctgga
     3121 ggaatggatc atcaaccgtc agaggcagag cgcagcactg acggatctga tggatcagta
     3181 cgtgctcctg gcataccgca caacggttga cgatagccgc aactgatgcc ggttgcttcc
     3241 ggaaggtgtg acaaaatacg gaaatgatga tgctcccggc ccccatgctc acctgcagag
     3301 cgtgttcgtc gtcatcatac tgagccatga cgggcgtctt aagggcatcc tgtcattgct
     3361 gagcaggcaa caccaccaga ggagccacgg acagggatgt tctttacagc ccgatacacg
     3421 ccaggttctc agcagactga atgtgcaggt gcggttattc accagaaatg gcatgctgcg
     3481 gtacataccc atcctggtga caatatgtac gtggcgacag cgatcccggc actgtggaag
     3541 agactcactc agcctgcagg atattctttc cggcatccgg gaatgtccgc ctgaatgcaa
     3601 acaggatgat atttgaatcc gtaatactgt tctttcatca taaataattt atgcagatac
     3661 agttaaccgt tacagtagcc ggtttgtttt accgggaaat aatatccgtc agactgatac
     3721 cggaacatat ctgaaacacg ttatgatgtt ttgttatcag aaatgttgta tattcttatc
     3781 cgtggcgaca gtttaatgcc ctctggtatc acctgataat gtatatcctg cgatgattcg
     3841 ttatatttac gggacacgcc tcttttcatt gtgctccttt tcagacttca ttttccgtaa
     3901 gtgttccccg gcataaagga ggtacctttc tggcgtttat aactctcttt atcggtgacg
     3961 ccggtaatac agggaatacc acaccgacaa acgaggtatt aacagacgat atttcagtgt
     4021 ccataatttt tctgtccggt taaataacgt cagtctgctg ccctctgact gtaatcatca
     4081 aagccggaat gcataccggg ttacaacgta aaaaaagtga tgctggcccc cggtgcagct
     4141 ggcgggcgaa acatgtttac ctttcaatac cccctgtctg cagtctccac tcttccatcc
     4201 tcttaagcta cgtcggccac atgagtgcac ttccatgata acaggtattc ctgtcagcat
     4261 aacgaagccg gttaccttcc cttcctcctg catttcctgt cagaatgact atacagcgcg
     4321 cagattgcct ttttattgac catcttaata
//
LOCUS       DAZXUK010000284         4343 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZXUK010000284 DAZXUK010000000
VERSION     DAZXUK010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.284"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..89)
                     /locus_tag="ACH3L8_003693"
     CDS             complement(<1..89)
                     /locus_tag="ACH3L8_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086622.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLG"
     gene            complement(197..2872)
                     /gene="adhE"
                     /locus_tag="ACH3L8_003694"
                     /gene_synonym="adhC"
     CDS             complement(197..2872)
                     /gene="adhE"
                     /locus_tag="ACH3L8_003694"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFT4086623.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
     gene            3265..3318
                     /gene="ychE"
                     /locus_tag="ACH3L8_003695"
     CDS             3265..3318
                     /gene="ychE"
                     /locus_tag="ACH3L8_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFT4086624.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            3349..3996
                     /gene="ychE"
                     /locus_tag="ACH3L8_003696"
     CDS             3349..3996
                     /gene="ychE"
                     /locus_tag="ACH3L8_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFT4086625.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(4154..>4343)
                     /locus_tag="ACH3L8_003697"
     CDS             complement(4154..>4343)
                     /locus_tag="ACH3L8_003697"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086626.1"
                     /translation="IQSNRDWLSMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITN
                     IISIILQQAQDYDVAKIT"
BASE COUNT         1085 a          999 c         1057 g         1202 t
ORIGIN      
        1 cccagagaag tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt
       61 ccaatgtgca taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact
      121 ggatcggtca actgatcctt aactgatcgg cattacccca gaaggggccg tttatattgc
      181 cagacagcgc tactgattaa gcggattttt tcgctttttt ctcagcttta gccggagcgg
      241 cttctttttt cgctgcagtt tcatcttcta cataatcacg accgtagtag gtatccagca
      301 gaatctgttt cagctcggag atcagcgggt aacgcgggtt agcgccggtg cactggtcat
      361 cgaatgcatc ttcagacagt ttatccacgt tcgccaggaa gtctgcttcc tgaacgccag
      421 cttcacggat agatttcgga atacccagtt cagctttcag cgtttccagc catgccagca
      481 gtttctcgat cttagcagca gtacggtcgc ccggtgcgct cagacccagg tggtcggcaa
      541 tttcagcata acgacggcga gcctgcggac ggtcatactg gctgaatgca gtctgcttgg
      601 tcgggttgtc gttcgcattg tagcgaataa cgttacaaat cagcagggcg tttgccagac
      661 cgtgcggaat atggaactgg gaacccagtt tgtgcgccat tgagtgacat acacccagga
      721 aggcgttcgc aaatgcgata cccgcgatag ttgctgcact gtgaacacgt tcacgcgcta
      781 ccggattttt agacccttcg tggtaggacg ctggcagata ttctttcagc agtttcagtg
      841 cctgcagagc ctgaccatca gagaactcag atgccagtac agaaacataa gcttccatgg
      901 cgtgagttac tgcgtccaga ccaccgaaag cacacaggga cttcggcatg tccataacca
      961 ggttggcgtc gacaatcgcc atatccgggg tcagcgcata gtccgccagc gggtatttct
     1021 gaccagtagc gtcgtcagtt acaaccgcaa acggagtgac ttcagaacct gtaccagaag
     1081 tggtggtgac agcgatcatt ttcgctttca cgcccatttt cgggaacttg tagatacgtt
     1141 tacggatatc cataaagcgc agcgccagct cttcgaagtg agtttccgga tgttcgtaca
     1201 taacccacat gatcttcgcg gcgtccatcg gggaaccacc acccagcgcg ataatcacgt
     1261 ctggtttgaa ggagtttgcc agttctgcac ctttacgaac gatgctcagg gtcgggtccg
     1321 cttctacttc gaagaagact tcagtttcaa cgcctgctgc tttcagtacg gaagtgatct
     1381 gatcagcata accattgttg aacaggaagc ggtcagtcac gatgagcgca cgtttgtggc
     1441 catcagtaat cacttcatcc agcgcgattg gcagggagcc acggcggaag tagatagatt
     1501 tcggaagttt gtgccacaac atgttttcag ctcgcttagc aacggttttc ttgttgatca
     1561 ggtgtttcgg accaacgttt tcagagatgg agttaccacc ccaggaacca caacccagag
     1621 tcagggaagg tgcgagtttg aagttataca ggtcaccgat accaccctga gaagccgggg
     1681 tgttaatcag gatacgagct gttttcattt tctgaccgaa gtaagaaacg cgagccggtt
     1741 ggttatcctg gtcagtgtac aggcaagagg tatgaccgat accgcccata gcaaccagtt
     1801 tctctgcttt ttctaccgcg tcttcgaaat ctttagcgcg gtacattgcc agagtcgggg
     1861 acagtttttc atgtgcgaac ggttcgcttt catcaacaac ggtcacttca ccgatcagaa
     1921 tcttggtgtt ttctggtaca gagaagcctg ccagttcagc aattttatag gctggctgac
     1981 caacgatagc cgcgttcagc gcaccatttt tcaggataac atcctgaaca gctttcagct
     2041 ctttaccctg caacagatag ccgccgtggg ttgcgaaacg ttcacgtact gcgtcataaa
     2101 cagagtcaac aacaacaaca gactgttcag aagcacagat tacgccgttg tcgaaggttt
     2161 tggacatcag tacagatgca actgcacgtt tgatatcagc agtttcatca ataacaactg
     2221 gagtgttacc cgcgcctaca ccgatagctg gtttaccgga gctgtatgcg gctttaacca
     2281 tgcccggacc accagtcgcg aggatcaggt tgatgtctgg gtggtgcatc agtgcgttag
     2341 acagttcaac agaaggttga tcgatccagc cgatcagatc tttcggagca ccggcagcga
     2401 tagcagcctg cagaacgata tcagccgctt tgttggttgc atcttttgca cgtgggtgcg
     2461 gggagaagat aatggcgtta cgggtcttca ggctgatcag cgatttgaag atagcagttg
     2521 aagtcgggtt agtggtcgga acgataccgc aaataatacc gattggttca gcgatagtga
     2581 tggtaccaaa agtgtcgtct tcagacagaa caccacaggt tttttcatct ttataggcgt
     2641 tgtagatata ttcagaagca aagtggtttt tgatcacttt atcttcgacg atacccatgc
     2701 cggattcggc aacggccatt ttcgcgagtg ggattcgagc atctgcagca gccagagcgg
     2761 cggcgcggaa gattttgtct acttgctctt gagtgaaact ggcatattca cgctgggctt
     2821 tttttacacg ctctacgagt gcgttaagtt cagcgacatt agtaacagcc ataatgctct
     2881 cctgataatg ttaaactttt ttagtaaatc atctgctcga atacgagagt atagtcagtg
     2941 cggtgatgat ttgcttaacc aatgaaaatc aaaagcttac tcgcgctcac actcactgtg
     3001 atttactaaa agagtttaaa cattagagtt attatctcta atgcgtcact tccaggtggc
     3061 gtaagcaaga ttactcactt ctgggtactg attacgtgat ccaaatcaaa tttttgcaaa
     3121 gctgacacct ttcagcatcg cttttcgcca ttatagctaa cagttaataa attgtagtat
     3181 gatttggtgg ctacattagc atgttttgca caactagata acaataacga atgatagcaa
     3241 ttttaagtag ttaggaggtg aaaaatgctg tcaaaaggcg tattgtcagc gcgtcttttc
     3301 aaccttattt atggctaaca ttatccggct tttgcttcgg agcgaaccgt gattcagacc
     3361 ctttttgatt ttcccgttta cttcaaattt ttcatcgggt tatttgcgct ggtcaacccg
     3421 gtagggatta ttcccgtctt tatcagcatg accagttatc agacagcggc agcgcgaaac
     3481 aaaactaacc ttacagccaa cctgtctgtg gccattatct tgtggatctc gctttttctc
     3541 ggcgacacga ttctacaact ttttggtata tcaattgatt cgttccgtat cgccgggggc
     3601 atcctggtgg tgacgatagc gatgtcgatg atcagcggca agcttggcga ggataaacag
     3661 aacaagcaag aaaaatcaga aaccgcggta cgtgaaagta ttggtgtggt gccattggcg
     3721 ttgccgttga tggcggggcc aggggcgatc agttctacca tcgtctgggg tacgcgttat
     3781 cacagcatta gctatctgtt tggtttcttt gtggctattg cattgttcgc tttatgttgc
     3841 tggggattgt tccgcatggc accgtggctg gtacgggttt tacgccagac cggcatcaac
     3901 gtgattacgc gtattatggg gctattgctg atggcattgg ggattgaatt tatcgttact
     3961 ggtattaagg ggattttccc cggcctgctt aattaattcc tttcaaatga aacggagctg
     4021 ccatgctccg tttacttcgt cattattttt actttgttcc cgcgcagtta tcaaaagcaa
     4081 aaggaatagg taaaaatatt cttctcaaat tacagttagt tataaggatt tccttaactg
     4141 cttctcctca ccatcatgtt attttcgcca catcataatc ctgggcttgc tgaagaataa
     4201 ttgaaatgat attagtaatt ccactgcctt tggtagagga aagtgctaaa taataatcaa
     4261 ttgttaaatt attgtgcatt tcactactgg aactgtaatc agaaaagata gacatgctta
     4321 gccaatctct atttgattga att
//
LOCUS       DAZXUK010000285         4318 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZXUK010000285 DAZXUK010000000
VERSION     DAZXUK010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.285"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(103..849)
                     /gene="cutC"
                     /locus_tag="ACH3L8_003698"
     CDS             complement(103..849)
                     /gene="cutC"
                     /locus_tag="ACH3L8_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFT4086627.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(863..1429)
                     /locus_tag="ACH3L8_003699"
     CDS             complement(863..1429)
                     /locus_tag="ACH3L8_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFT4086628.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1645..3378
                     /gene="argS"
                     /locus_tag="ACH3L8_003700"
     CDS             1645..3378
                     /gene="argS"
                     /locus_tag="ACH3L8_003700"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFT4086629.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3420..3464)
                     /locus_tag="ACH3L8_003701"
     CDS             complement(3420..3464)
                     /locus_tag="ACH3L8_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086630.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            3555..4043
                     /locus_tag="ACH3L8_003702"
     CDS             3555..4043
                     /locus_tag="ACH3L8_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFT4086631.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4161..4229)
                     /locus_tag="ACH3L8_003703"
                     /pseudo
     CDS             complement(4161..4229)
                     /locus_tag="ACH3L8_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
BASE COUNT         1131 a         1069 c         1055 g         1063 t
ORIGIN      
        1 cgttttttat actgctcagg gataaaaaaa ggcccctgtt gaaattgcag gggcctggta
       61 cgagcaagca tcatattggg cgacatgatg caacggtaaa aatcatttgg cctgatggcg
      121 ttcaatgatt cctttcattt cagcaaccgc cgccccgtct acgatataac gcgaatactc
      181 gtccgcgtgt tcatctgatg acatggacaa tccttgatta cgataacgca tcggtgaggc
      241 ttgccacgct cccgcggagc tatggacttc cagcactccg gcatcgagga agtggtgcag
      301 gttttctgca cggactcctg ctccagccat aatgattgga gcatcacgat gggcaataag
      361 ttccataatt tttgataaac cttgcagtgc gtctgatttt tgccctgatg tcagtacccg
      421 ggcaatgccc aattccgcaa gattattgag tgtatataaa gggttagcgc acatatcgaa
      481 ggcgcgatga aaagtcactg ccagcggacc ggcagcagcc attatttttt ccattcgtgg
      541 catatcgaca ttcccgtcaa catcgagaac gcccgtcacc agtccaggaa aacctaattc
      601 gcggaccgtg cgcacatcct caagaatggc ggcaaactca ccgtcgctgt agcaaaaatc
      661 accaccgcgt gggcgaatta tcggatgcac agggatcgtc acccgctggc gcacggattt
      721 cagtaccccc aacgacggcg ttaagccccc ctcttttggg gctgcgcata attcaactct
      781 gtctgcgccg ttttgctgcg ccgttagtgc acattccatg ctgtaacagc aaatttccag
      841 taatgccatt tttgctcctt aattacgctg actgctcgct ggcaacgatc tcttcaatgg
      901 accacggatg aaacttaata gttgttttgc catcggtgac cgccagcgtc ggattcggta
      961 atcgctcatg ctcacctttt ggtgaactgg ttttaactga gatccccggt aggctgagtc
     1021 cttcatcaga aagcaatgcc agcgcacggg cattcagcgt ttctggatca cccggcagaa
     1081 cgatttcaat atgttcccaa ccttcgtgtg ggtaacgttt ttccccgggc cacggtagct
     1141 ccacattgga aaactgccag tgcgcaacct gtaccggttc atgcaattta aacagacaaa
     1201 tcggtctgcc attgatcatt ttttctgaca aaagctcgcc acactgttca aaaccgcgac
     1261 gccagcgctc agcggtgacg ttttgatggc aacgcaaaga aatgtgatcg gcagtcagcg
     1321 gagtgatatc caaccccaga cggcgggaaa gttcatctaa tgcgtgggta aatcgcggta
     1381 aatccgatgc aatatcctgc agctcgtcga tagattgcca gttcgccata atcactcttc
     1441 gtctttcagt aaaagcgtta atttaccctg ttgccctgtg ccaaccaacc gctgatttca
     1501 cgccgcttct gatgcaatag tgaaaacggc aatacgccac gcgcacgttg ctgacgaaaa
     1561 cagccatttg cagtatactc ccgccctaat ttctttaact ggtgcgggca atttttgctc
     1621 gcttcatcaa tgtaaggtat tccggtgaat attcaggctc ttctctcaga aaaagtccgt
     1681 caggccatga ttgcggcagg cgcgcctgcg gattgcgaac cgcagattcg tcagtcagca
     1741 aaagttcagt tcggcgacta tcaggctaac ggcatgatgg cagttgctaa aaaactgggt
     1801 atggcaccgc gacaattagc agagcaggtg ctgactcatc tggatcttaa cggtatcgcc
     1861 agcaaagttg agatcgccgg tccaggcttt atcaacattt tccttgatcc ggcattcctg
     1921 gctgaacatg ttcagcaggc gctggcgtcc gatcgtctcg gtgttgctac gccagaaaaa
     1981 cagaccattg tggttgacta ctctgcgcca aacgtggcga aagagatgca cgtcggtcac
     2041 ctgcgctcta ccattattgg tgacacagca gtgcgtactc tggagttcct cggtcacaaa
     2101 gtgattcgcg caaaccacgt cggcgactgg ggcactcagt tcggtatgct gattgcatgg
     2161 ctggaaaagc agcagcagga aaacgccggt gaaatggagc tggctgacct tgaaggtttc
     2221 taccgcgatg cgaaaaagca ttacgacgaa gatgaagagt ttgccgagcg tgcacgtaac
     2281 tacgtggtaa aactgcaaag cggtgacgaa tatttccgcg agatgtggcg caaactggtc
     2341 gacatcacca tgacgcagaa ccagatcact tacgatcgtc tcaacgtgac gctgacccgt
     2401 gatgacgtga tgggcgaaag cctctacaac ccaatgctgc caggaattgt ggcggatctc
     2461 aaagccaaag gtctggcagt agaaagcgaa ggggcgaccg tcgtattcct tgatgtgttt
     2521 aaaaacaagg aaggcgaacc gatgggcgtg atcattcaga agaaagatgg cggctatctc
     2581 tacaccacca ctgatatcgc ctgtgcgaaa tatcgttatg aaacactgca tgccgatcgc
     2641 gtgctgtatt acatcgactc ccgtcagcat caacacctga tgcaggcatg ggcgatcgtc
     2701 cgtaaagcag gctatgtacc ggaatccgta ccgctggaac accacatgtt cggcatgatg
     2761 ctgggtaaag acggcaaacc gttcaaaacc cgcgcgggtg gtaccgtgaa actggcagac
     2821 ctgctggatg aagccctgga gcgtgcacgt cgtctggtgg cagaaaagaa cccggatatg
     2881 ccagctgacg agctggaaaa actggctaac gcggttggta ttggtgcggt gaaatatgcg
     2941 gatctctcca aaaaccgcac cacggactac atcttcgact gggacaacat gctggcgttt
     3001 gagggtaata ccgcgccata catgcagtat gcttacacgc gtgtattgtc cgtgttccgt
     3061 aaagcagaaa ttaacgaaga gcaactggct gcagctccgg taatcatccg tgaagatcgt
     3121 gaagcgcaac tggcagctcg cctgctgcag tttgaagaaa ccctcaccgt ggttgcccgt
     3181 gaaggcacgc cgcatgtaat gtgtgcttac ctgtacgatc tggccggtct gttctctggc
     3241 ttctacgagc actgcccgat cctcagcgca gaaaacgaag aagtgcgtaa cagccgtctg
     3301 aaactggcac aactgacagc gaagacgctg aagctgggtc tggatacgct gggtattgag
     3361 actgtagagc gtatgtaatc gatttttcgt gagagtgaag cctgatcagg gttagccgat
     3421 caggcttttt tattgccatc taaatgtatt ctgaaaacgg acatgccatt gttttctcac
     3481 tgttgagtaa gaggccagaa gcgtaatatc caggcccagg aaaacgataa cggttcaatt
     3541 taaggaatac cgaagtgttt aaatttcttg tattaacatt aggcattatc tcttgccagg
     3601 cttacgcaga agatacggtt atagtaaacg accatgacac ttcagccatc aaagattgtt
     3661 ggcaaaaaaa ttcagatgat gatactgaca ttaacgtgat caaatcatgc ctgcgacaag
     3721 aatacaatct cgtcgatgcg caattaaata aagcctatgg tgaagcttat cgttatatag
     3781 aacaagtgcc acgcacaggt gcaaaaaaac ttgatactga acaacttaac ttgcttaaaa
     3841 aatcacagcg agcctggctg gattttaggg acaaagaatg tgaattaatc ctttcaaatg
     3901 aggatgttca ggatttaagt gacccctatt ctgaatcaga atggctatca tgtatgatca
     3961 tacagaccaa tacgcgaact cgccagttgc agctataccg taactctgaa gatttttatc
     4021 caagcccttt gacaagagga taattcacat ctttttggca tgttttgttg caagctattc
     4081 ctgataaatg attgcaacaa aacatccagc cttttgactg agttattaaa catactcgcg
     4141 agcgcgtaat tttttgtctt tcagcgataa ttcacaatca cttcattccg ttgtaccttt
     4201 agcggtggaa ttaaccgccc tgaaccgccc cgggtttcct ggagagtgtt ttatctgtga
     4261 actcaggctg ccagatcatc gtttccgatg gaagcataat aagctttttc tgcttctg
//
LOCUS       DAZXUK010000286         4308 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZXUK010000286 DAZXUK010000000
VERSION     DAZXUK010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.286"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..118
                     /locus_tag="ACH3L8_003704"
     CDS             <1..118
                     /locus_tag="ACH3L8_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086632.1"
                     /translation="NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(115..381)
                     /locus_tag="ACH3L8_003705"
     CDS             complement(115..381)
                     /locus_tag="ACH3L8_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFT4086633.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            655..4179
                     /gene="nifJ"
                     /locus_tag="ACH3L8_003706"
     CDS             655..4179
                     /gene="nifJ"
                     /locus_tag="ACH3L8_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFT4086634.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1045 a         1132 c         1121 g         1010 t
ORIGIN      
        1 taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt
       61 aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata
      121 gctgccacag ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc
      181 acacatccca atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat
      241 tgcacaattt gcttctccac tactggacat cgacgctttt aaacttggcg ctacgtgcgc
      301 cgcagtcgcc tgctgaacag gctcactact acacgccgac aacaataaag cggcacaccc
      361 tacccaaaac gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca
      421 taaataatag gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt
      481 ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat tactctgatg
      541 tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta
      601 ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt
      661 actattgacg gtaatggcgc ggttgcttcg gtgacgtttc gcatcagtga agttatcgcc
      721 atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac
      781 ggcttgaaga acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt
      841 gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca
      901 tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actgacgcca
      961 tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactttctat ttttggcgat
     1021 cattccgacg ttatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc
     1081 caggaagcgc aagactttgc tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca
     1141 tttattcatt tctttgatgg ttttcgcacg tcccacgaaa tcaataaaat tgtcccgctg
     1201 gccgatgaca ctattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg
     1261 gcgctcaacc cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc
     1321 cagtctcgcg aagccaccaa tccctggtac aacgcggtct atgaccatgt tgaacaggcg
     1381 atgaatgatt tcgctgccgc gacaggtcgt cagtatcagc cgtttgagta ttacgggcat
     1441 ccgcaagcgg aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg
     1501 gttgatgaat tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg tctgtaccgt
     1561 cccttctccg ccaaacactt gctgcaagct ctgccgggat ccgtacgcag cgtggcggta
     1621 ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtgatgacc
     1681 gcactggcag aagcctttaa taatggtgag cgcgaaaccc tgccccgtgt cattggtggg
     1741 cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag
     1801 ctcaacgcgg ctaaacctaa agcacgcttt acggttggta tttacgatga tgtgaccaat
     1861 ctgtcactgc cgttgccgga aaacactctg ccaaactcgg cgaaacttga agccttgttt
     1921 tatggccttg gtagtgatgg cagcgtttcc gcaaccaaaa acaatatcaa gattatcggt
     1981 aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa agcgggcggc
     2041 ctgacggttt ctcaccttcg cgtgagcgaa cagccgattc gttccgctta tctcatttcc
     2101 caggctgatt ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag
     2161 cgtttaaaac ctagcggcat tttcctgctt aatacgccgt acagcgcaga tgaagtgtgg
     2221 tcgcgcttgc cgcaggaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg
     2281 attaacgcgg cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg
     2341 cagatggctt ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg
     2401 cagggtgcga ttgccaaaag ttacagtagc aaaggtcagg atctggtgga gcgcaactgg
     2461 caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg
     2521 cacagcgcca atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta
     2581 accgcggcga tgctcgccgg gcttggtgat gccctccccg tttcggcgct gccgccagac
     2641 ggcacctggc cgatgggcac cactcgctgg gaaaaacgca atatcgccga agagatcccc
     2701 atctggaaag aggaactctg tacccaatgt aaccactgcg tcgccgcttg cccacactca
     2761 gctattcgcg caaaagtggt accgcctgaa gcgatggaaa acgcccctgc cagcctgcat
     2821 tcgctagatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg
     2881 gaagattgca ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat
     2941 ccagagatta aagccatcaa tatgatgtct cgccaggaac atgtcgaaga agagaaaatc
     3001 aattacgatt tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat
     3061 attcgtacat cgcagctgat tacaccgctg tttgaatatt caggtgcttg ccccggttgt
     3121 ggcgagacgc cgtatattaa attgctgact cagctctatg gtgaccggat gttgatcgct
     3181 aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc
     3241 gatgccaacg gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt
     3301 ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat
     3361 caatttgccg ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca
     3421 gaggttcgtc gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca
     3481 catgaacttc tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc
     3541 gacggctggg cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa
     3601 aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg
     3661 aaagcgacac cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt
     3721 aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc
     3781 ggcgcgcagc tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccgggacca
     3841 tcgctgatca ttgcttatag cccgtgtgaa gagcatggtt acgatctggc tctcagccac
     3901 gaccagatgc gccaactcac tgctaccggc ttctggccgc tatatcgctt tgatccgcgt
     3961 cgtgccgatg aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg
     4021 gaagaaacgt tacttcacga gcagcgtttc cgtcgtctga attcgcagca gccagaagtg
     4081 gcagaacagt tatggaaaga tgctgcagct aatttgcaaa aacgctatga cttcctagca
     4141 caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc
     4201 caattctaaa aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat
     4261 cagtggcaat gcaatggccc aacagaaaat aaaatctgtg acaataaa
//
LOCUS       DAZXUK010000287         4306 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZXUK010000287 DAZXUK010000000
VERSION     DAZXUK010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.287"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..434)
                     /gene="fabA"
                     /locus_tag="ACH3L8_003707"
     CDS             complement(<1..434)
                     /gene="fabA"
                     /locus_tag="ACH3L8_003707"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFT4086635.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLA"
     gene            complement(503..2263)
                     /locus_tag="ACH3L8_003708"
     CDS             complement(503..2263)
                     /locus_tag="ACH3L8_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFT4086636.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            2448..2900
                     /gene="matP"
                     /locus_tag="ACH3L8_003709"
     CDS             2448..2900
                     /gene="matP"
                     /locus_tag="ACH3L8_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFT4086637.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(2976..4022)
                     /gene="ompA"
                     /locus_tag="ACH3L8_003710"
     CDS             complement(2976..4022)
                     /gene="ompA"
                     /locus_tag="ACH3L8_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFT4086638.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
BASE COUNT         1104 a         1105 c         1062 g         1035 t
ORIGIN      
        1 tctgccagac ccataatcag acgacgatta acaatgcgtt taaagtgaat acggtaggtc
       61 acttttttcg ctgtcggcag tacctgacca gtgaatttca cttcgccaac gcccagcgcg
      121 cggcctttac cttcgccgcc cagccagccg aggtagaacc ctaccagctg ccacattgcg
      181 tccaggccca ggcatcccgg cataaccgga tcgccaatga agtggcatcc gaagaaccac
      241 agatccggat tgatatccag ttctgcttca acataccctt tgtcgaagtt accacccgtt
      301 tcggtcattt tgaccacacg gtctatcatc agcatgttcg gtgctggcaa ttgcgggcct
      361 ttagcgccaa acagttcacc gtgaccagag gcaagaaggt cttcttttgt ataggattcg
      421 cgtttatcta ccatgttctc tgtaagcctt attttattga agcacgcagg atagctaaca
      481 cgtgtacgct gaacaagtcc gatcagttcg gaataaacca gttcagccaa cgtaatggcc
      541 atggaaaacg gtgacgtcct tcctgttgcg atgcttgcgc gatacgttcc tggatggttt
      601 gcatcagcgt cgtttggcct tcgccatccc acaccagatt taataataac ggcagtgcgt
      661 cagtcacatc gtctactgcc cagatggtga atttgccttc ttctaccgct ttcaccagtt
      721 cactgtgaag acttaaatgg cgaacgttag cagtggggat aatgacacct tgtttccccg
      781 ttaactcacg ttgctggcaa atagcaaaga agccttcgat tttctcattt aaaccaccga
      841 ccggctggac gcggccgaac tgatcgactg aacctgtgat agcgatactc tgattcactg
      901 gcacatcagc gagggcgctt atcagggcgc agagttcagc catcgaggca ctatcgccat
      961 ccacttcact gtatgactgc tcaaatgtca gcgatgctga gaaggggatc tgttgctcaa
     1021 gctgtagttc cgacatcagg aacgcctgca tgatcatcat ccctttcgca tggatattgc
     1081 cgccaagctc cgctttgcgt tcgatgtcgg tgaattcacc atcgccaata tgcacaacgc
     1141 agctaatgcg agaaggttcg ccaaaagcgc gtggatgacc cggaaattca atgaccgaaa
     1201 gggcgttaat ttgcccgatg cgttcgcctt cggtttcaat caggatttgc tcctgaagga
     1261 tctcatcctg catacgttca gcgaggaaac cttcgcgcca ttcacgctgc tgcagcatta
     1321 agtttagctg ctcgccggag aaggtgtcgc catcacacag ggaggcgacc tctttacact
     1381 ggcggaggat ccactgcggg ctaagcggaa gtgtttcttg ttcaccggtg tagcgtgctg
     1441 cttcgcggat aagtaccggc caagcatccg ctcccggggc aggtaagtga ttatgtctgg
     1501 cggtaaatgt cacccagcga caccactggc ttactgactc cgcatcgaca atctgcagag
     1561 tatcttcaaa ttcgctataa atagcctgct ctgaaagctc tggctccatc tcctggaaat
     1621 cagccaatga ttcgcgttcg cctaccagaa tgactttcag cttcaatggc attgaaggca
     1681 cagagacggg gagagggcgc gactcatcaa acgcaaccca gtcaaaacgc tcgcggttaa
     1741 cgatattttt cagccgcatc cacagcagag gttgcgccag cagtgtacgc aaagagatga
     1801 tgagaatacc gccatttgcc tgatgcacca gaccaggctg cagggtaatg tcgccattaa
     1861 actggcgcag gcagccaaag agttgctccg cttctaccca gtcggcagcg acaacttgcg
     1921 ttaaagtcgc aaaattatca tctgcactca ctgcgtgacg taagcggatg gagtggccgg
     1981 aaacctcata gtgatcgccc accagttggc ctgcatcgct ttgtagcgta cgcgcggcat
     2041 cggcaataag attcagatac tcagactctt ccggggcctt agccagcata aaagaggagg
     2101 atgctcgcgt atgcagcaac tgctccagcg caaattgcag tcgcggttga gtatcactga
     2161 ataaaggatc gttttcgtca atcaaatgtg gctgagcaaa tatttcctga tagctatcgg
     2221 tatcaggaac caggtcacgc catgcaagtt tcgtaatggt caaagttgat gttttttagt
     2281 ctgttgtcaa agccgcgatt ataccgtaac cggcactaca gcacacgtag aaagcaccga
     2341 caatactcct ggctgggcgt taaagctcac aggatggaga ttcttttctt cactggccta
     2401 aaaagctgat attctgtaaa gagttacacg gtaacattga gatcgctatg aaatatcaac
     2461 aacttgaaaa tcttgaaagc ggttggaaat ggaagtatct ggtgaagaag catcgcgaag
     2521 gggagttaat tacccgttac atagaagcca gtgccgccca ggaagccgtc gatgtgttgc
     2581 tctcgctcga aaatgaaccc gtgctggtaa atggctggat tgacaagcat atgaatccgg
     2641 aactggtcaa tcgcatgaag cagaccattc gggccagacg gaagcgccat tttaatgcgg
     2701 aacaccagca tacgcgcaaa aaatcgatcg atctggaatt tattgtctgg caacgtctgg
     2761 ctggtcttgc acagcgtcgc ggtaaaacgc tttctgaaac gattgttcag ctgattgaag
     2821 atgcggaaaa caaagagaaa tacgcgaata aaatgtcttc tcttaagcag gatctgcagg
     2881 cattgctggg taaggaataa ctgacgaaag tcagttcaat ttactaaagg caaaaaaaac
     2941 cccgcagcag cggggttttt ctaccagacg ataacttaag cctgcggctg agttacaacg
     3001 tctttgatac ctttaacttc gatctctacg cgacgatccg gagccaagca gtcgatcagt
     3061 gcagcacgct gtttcacgtt gtcacaggtg ttgccagtaa ccgggttgga ttcgcccata
     3121 ccacgtgcgg agatcttgtc tgccgggata cctttggaga tcaggtaatc aacaacagac
     3181 tgagcacggc gctcggacag accctggttg taagcgtcag aaccgatgcg gtcagtgtaa
     3241 cccagaacaa ctacggaacc gtctttcgga tctaggttgc tcagctggct gtacagctga
     3301 tccagagcag cctgaccttc cggtttcagg gttgctttgt tgaagttgaa cagaacgtca
     3361 gacttcagag tgaagtgctt ggtctgtact tccggtgccg gagctggagc aactaccgga
     3421 gctgcttcgc cctgaccgaa acggtaggaa acacccaggc tcagcagacc gttgtccgga
     3481 cgagtaccga tggtgtttgc gtcaccgatg ttgttggtcc actggtattc cagacgggta
     3541 gcgatttcag gagtgatcgc gtactcaaca ccacctgcga aaaccggaga aacgccagtg
     3601 tcgtggtctt taaaggatgc gccaccaggt acgttagcct tggtgtctgc acgccatacc
     3661 ataccaccca gacgagtgta gatgtccaga tcgtcagtga ttgggtaacc cagtttagcg
     3721 gtcagctgaa cgccctgagc tttgtatgcg ccgttgatgt tgtcgccttt gtacggcata
     3781 cgacctaacc agtcgtaacc catttcaaaa ccaacatacg ggttaacctg gtaaccacca
     3841 aaagcacctg cacccagttg gttttcgtgg gtcggaccat tgttaggaat aaaaccagtg
     3901 tcatggtact gggaccagcc cagtttagca ccagtgtacc aggtgttatc tttcggagcg
     3961 gcctgcgcta cggtagcgaa accagccagt gccactgcaa tcgcgatagc tgtctttttc
     4021 attttttgcg cctcgttatc atccaaaata cgccatgaat atctccaacg agataacacg
     4081 gttaaatcct tcaccggggg atctgctcaa tattaactct accgatatct tcggcttatg
     4141 ccgagcaccc ctggcgatgt aaagtctaca acgtagttga aaacttacaa gtgtgaactc
     4201 cgtcaggcat atgaaaaaaa agtcttgtat aaggtatgtt taatcttttt gtcagcgaca
     4261 atttacagaa aaaaaacgcg gaaaccgctt cagacaagcc ttcgca
//
LOCUS       DAZXUK010000288         4285 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZXUK010000288 DAZXUK010000000
VERSION     DAZXUK010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.288"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            49..1254
                     /gene="yedE"
                     /locus_tag="ACH3L8_003711"
     CDS             49..1254
                     /gene="yedE"
                     /locus_tag="ACH3L8_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFT4086639.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            1251..1484
                     /gene="yedF"
                     /locus_tag="ACH3L8_003712"
     CDS             1251..1484
                     /gene="yedF"
                     /locus_tag="ACH3L8_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFT4086640.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            1591..2261
                     /locus_tag="ACH3L8_003713"
                     /pseudo
     CDS             1591..2261
                     /locus_tag="ACH3L8_003713"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(2301..3509)
                     /locus_tag="ACH3L8_003714"
                     /pseudo
     CDS             complement(2301..3509)
                     /locus_tag="ACH3L8_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            3517..3943
                     /gene="tnpA"
                     /locus_tag="ACH3L8_003715"
                     /pseudo
     CDS             3517..3943
                     /gene="tnpA"
                     /locus_tag="ACH3L8_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          977 a         1091 c         1075 g         1142 t
ORIGIN      
        1 tgattcgcgg ctcttgtttc aaaccttctg agttcagagg ctacattcat gtcatggcag
       61 caattcaaac acgcctggtt gattaaattc tgggcaccca tccctgcggt catcgcggcg
      121 ggtattctct ccacttacta ttttggcatt actggcacct tttgggctgt cacgggtgaa
      181 tttacccgtt ggggcggtca gctcctgcag ctgtttggcg tccatgctga agagtggggt
      241 tattttaaaa ttatccatct ggaaggatcg ccattaaccc ggatcgacgg catgatgatc
      301 ctcggtatgt ttggcggctg ctttgctgct gcgctgtggg ccaacaatgt caaactacgc
      361 atgccacgca gccgtatccg cattatgcag gccatcattg gcggcattat cgccggtttt
      421 ggcgcgcgtc tggcaatggg ctgtaacctg gcggcgttct ttaccggcat tcctcagttc
      481 tcgctgcatg cctggttctt tgccatcgcc actgccattg gttcatggtt tggcgcgcgc
      541 tttaccctgc tgcccatctt ccgtattccc gtgaaaatgc agaaagtttc tgccgcctca
      601 ccgctgacgc aaaaaccgga tcaggcgcgg cgtcgttttc gtctcgggat gctggtcttt
      661 ttcggcatgc tgggctgggc gctgctcacg gcgatgaacc aaccaaaact ggggctggca
      721 atgctgtttg gcgtcggctt tggtttactg attgaacgtg cgcaaatctg ctttacttcg
      781 gcgttccgcg atatgtggat caccggacgt acccatatgg cgaaagcaat cattatcggt
      841 atggcggtga gtgccatcgg gatcttcagt tacgtacagt taggcgttga acctaaaatc
      901 atgtgggcgg gaccaaatgc ggtaattggt ggtttacttt ttggttttgg catcgtgctg
      961 gcaggcggct gcgaaaccgg ctggatgtac cgcgcggtag aaggccaggt gcactactgg
     1021 tgggtcggtc tgggcaacgt gatcggctca acgattctgg cgtattactg ggatgatttc
     1081 gctccggcgc tggccaccga ctgggacaaa atcaacctgc tgaaaacttt tggtccgatg
     1141 ggaggcctgc tggtgacata tttgctgctg tttgctgcgc tgatgttgat tatcggctgg
     1201 gaaaaacgct tcttccgccg tgaggcaccg cagactgcta aggagatcgc atgaaaaata
     1261 tcgttcctga ttaccgcctt gatatggtgg gtgaaccctg cccttatccg gcagtcgcaa
     1321 cccttgaggc gatgccgcaa ttaaaaaaag gtgagatcct ggaagtggtg agcgactgtc
     1381 cgcagtcgat caataatatt ccactggatg cgcgtaatca cggctacacg gtgctggata
     1441 ttcagcaaga cgggccgacc attcgttatt taattcagaa gtaatccctc attcccgctg
     1501 tggcccacag cgggaacatc gttacctctg acacatagat tttacccgcc ccctctccta
     1561 ttcttttcag tatgatctca ggaggttgct atgtgtggac gctttgccca atcccaaacg
     1621 cgtgaggatt accttgcgct tctcgcggaa gatattgaac gcgatattcc ctacgatccc
     1681 gaacccattg gcagatacaa tgtcgcgccg ggaaccaaag ttcttctgct cagtgaacgt
     1741 gatgaacacc ttcatctgga tccggttttc tggggatatg ctcccggatg gtgggataaa
     1801 ccgccgctga ttaacgcccg cgtagaaact gcctcaacca gtcgcatgtt taaaccgctc
     1861 tggcaacatg gtcgggcaat ctgttttgcc gatggctggt ttgagtggaa aaaagaaggc
     1921 gacaaaaaaa gccttatttt atctatcgcg ctgacggaca acctgttttt atggccgcga
     1981 taggtagcat accgtttgag cgtggtgacg aagccgaagg atttttgatc gtcactgcgg
     2041 cggcagatca aggtctggta gatattcatg accgccgccc gctggttctg tcgccagaag
     2101 ctgcacggga atggatgcgg caagacattg gtggtaaaga agcctcagaa atcgctacca
     2161 gaagctgtgt gccagcaaac cagttcacct ggcaccctgt gtcgcgcacg gtgggtaatg
     2221 tcaaaaacca gggcgcggag ttaattcaac ctgtttgttg acatcctcca cgccctgaat
     2281 gacgaggacc cctgctacgt tcaggctgtc gcctgaatca tttcggtggg ttcctgcttc
     2341 aacgagcggc ctgactgcac catctctcca caggcaagaa cggcgtgccc cgccgctaaa
     2401 atgttacgag ctccatttac atcggcgttc gctgtatatc cacatacctg gcatctgaat
     2461 ttactttgtg acaggcgatt ttctttcgct gtatgaccac agcacgcgca acgctggctt
     2521 gtgtacgctg gcggaacagc aagcacctga ccgccacgcc agagctgctt gtactcaagc
     2581 tggcggcgca tttcatacca gccctgatcc agtatcgaac ggtttaaacc tgattttgcc
     2641 cggacattgc gccccggctg gctgaccgta cccgctgctg actttgacat gttgctgacc
     2701 ttcaaatcct caatgactat cattacgtgg tttttgctga cggtcgttgt gactttgtga
     2761 aggtagtccc tgcggatatt tgcgatacag gaatgcagtc gctgtatttt gcgtttctgc
     2821 ttctgccagt tgttgctgaa tttgaccttg cggcttaact gtcgctgaag tctcgccagc
     2881 gtcttctggt ttttctgaaa actgtttaca ggctcaaaga ctgtgccatc tgacagcgag
     2941 gcaagtttag ccacgccagc atccagcccg accattgatg ctgaagggtg aaccggagtt
     3001 gatacttcac tttctgcctg aatactgatg taccacttac cgcaggactg gctgacagtg
     3061 acatttttca caacacccgt gacctgacgg ctgttccggt agcgcatcca gccaagtttt
     3121 ggcagaaaaa tacggctgtt ttcctgagcg agcttaacgc cctgcggata gcggaatgca
     3181 tcattctgtc accgcttttt gaatcagggg aacgccgagc gcttctggaa aaagtttttg
     3241 caggcctgct cagggtcttt cagtgactgt tgcaatggct gtgacggagc atctttaatc
     3301 cattgcgttt cagtggcatt tttccactca accaatcagg aagccatttt cgcgtaaggg
     3361 atgtctttgt taccggcctt atgattctca ttctgacgtg ccagagtacg actgaaaacg
     3421 aaacgacatg cgcctgcgaa gcgcctcatt tcacgcgcct gttgaccacc gggtcttaac
     3481 cggaatttga atacctgtag tcgtttcaca gggcaaatta tactttggtc tgtgaaaaaa
     3541 actgatatcc gacgcggaag gcactgtgtt ttcctgatgc atgctcaccg ggtctttgtt
     3601 accaagtacc gacgccagat ttttgaccat gacgcgacag aaaaactacg cacttacttt
     3661 tcaaatgtat gtgcttattt tgaagctgaa ctggttgaaa tggatggcga accagatcac
     3721 gtccatctgt taattaacta tccccccaaa ctggcgatat ccagtctggt aaacaaacag
     3781 cctcaaaggc gtatcgggta ggttactgcg acgggatcga ccagatattg ctgccaggta
     3841 ttaccgcaaa gatgttttgt ggcgtcccgg ctattttgcc agtagctgcg gaggtgcgcc
     3901 aatatccgtc atccgccaat acattgaaca acagcaaaca cctggtcaga tgaaaaaacg
     3961 agccttatat ccccgccctg aaggacgggc ttttacggtg cactggataa gcctggagat
     4021 tatctacacc tgcatggaca tcattgtacg atgccccgta catgctccat cacatcctca
     4081 acatcaaagt attagagcct tgcaaaccat tatgcgtctc agtttgtctc atctatctta
     4141 cgcaaaaaaa atagaactaa tatcaagcgg cgctatattg cgtattaaat aaaaccacct
     4201 caattacatt aataaatcta aataaatatc atgcaaataa tttccatggt tatttttagg
     4261 ctatttaaat taaaaaaata aatta
//
LOCUS       DAZXUK010000289         4242 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZXUK010000289 DAZXUK010000000
VERSION     DAZXUK010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.289"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            171..1235
                     /gene="ulaG"
                     /locus_tag="ACH3L8_003716"
     CDS             171..1235
                     /gene="ulaG"
                     /locus_tag="ACH3L8_003716"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFT4086641.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1343..2098
                     /gene="ulaR"
                     /locus_tag="ACH3L8_003717"
     CDS             1343..2098
                     /gene="ulaR"
                     /locus_tag="ACH3L8_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFT4086642.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2095..2844)
                     /gene="yjfP"
                     /locus_tag="ACH3L8_003718"
     CDS             complement(2095..2844)
                     /gene="yjfP"
                     /locus_tag="ACH3L8_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFT4086643.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3026..3355
                     /gene="bsmA"
                     /locus_tag="ACH3L8_003719"
     CDS             3026..3355
                     /gene="bsmA"
                     /locus_tag="ACH3L8_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFT4086644.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3504..3779
                     /gene="yjfN"
                     /locus_tag="ACH3L8_003720"
     CDS             3504..3779
                     /gene="yjfN"
                     /locus_tag="ACH3L8_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFT4086645.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3896..>4242)
                     /locus_tag="ACH3L8_003721"
     CDS             complement(3896..>4242)
                     /locus_tag="ACH3L8_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFT4086646.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
BASE COUNT         1131 a         1060 c         1062 g          989 t
ORIGIN      
        1 agttaattta actacacatt ccattaaatc tttccccact caaaatcaac aacaacttcc
       61 ctggcaaaaa gtcaaaaaat ggtcattacc ttcctgaagg aatagtgtca ggattaatca
      121 aaaccaatca catgttgatt cgaattgaac tatcatgaag gtaaatggcg atgagtaaag
      181 tgaaaagtat cacccgagaa tcctggatcc tgagcacttt cccggagtgg ggtagctggc
      241 tgaatgaaga aattgaacaa gagcaggtcg cccctggcac atttgcgatg tggtggctag
      301 gctgcaccgg gatctggttg aaatcagaag gtggcaccaa cgtttgcgtt gatttctggt
      361 gcggcactgg caaacaaagt cacggtaacc cgttaatgaa acagggtcac cagatgcagc
      421 gcatggctgg cgtgaaaaaa ctgcagccaa acctacgcac caccccgttt gttcttgatc
      481 cgtttgcgat tcgccagatc gacgcggtac tggcgactca cgatcacaac gatcatatcg
      541 acgttaacgt cgctgctgcc gtgatgcaga actgtgctga tgacgtacca ttcattggac
      601 cgaaaacctg cgttgatttg tggattggct ggggcgtacc gaaagagcgt tgcatcgtgg
      661 tcaaaccggg cgatgttgta aaagtgaaag acattgaaat tcatgcgctt gacgctttcg
      721 accgtactgc actgatcacc ctgcctgccg atcaaaaagc ggctggcgta ctgccagatg
      781 gcatggacga tcgcgcagta aactacctgt tcaaaacccc tggcggttcc ctatatcaca
      841 gtggcgattc ccactactct aactattatg cgaagcacgg taacgaacat cagatcgatg
      901 tggcgttagg atcgtatggt gagaacccgc gcggtattac cgacaaaatg accagcgccg
      961 atatgctgcg tatgggtgaa gccctgaatg cgaaagtggt tatcccgttc caccacgaca
     1021 tctggtcaaa cttccaggcc gatccgcaag agatccgcgt gctgtgggag atcaaaaaag
     1081 atcgtctgaa gtatggcttc aagccgttta tctggcaggt tggcggcaaa tttacctggc
     1141 cgctggataa agacaacttc gagtaccact atccgcgcgg tttcgatgat tgcttcacta
     1201 ttgaaccgga tctgccgttc aagtcattcc tgtaatctgt cgtttatacc ggatagcgtg
     1261 gagaagttcg cgctatccgg cgtagtattt cctggctatt tcaaatatca tctaaaaaaa
     1321 tcaaatttta tcggaatagc tcatgactga agcacaaaga catcaaatcc tcctggaaat
     1381 gctcgcacaa ttgggctttg tgaccgttga gaaagtcgtt gagcgtctgg gaatttcgcc
     1441 tgccactgcg cgacgcgata tcaataaact tggcgaaagc ggcaaactga aaaaggtgcg
     1501 caatggcgca gaagctatta cccaacagcg cccgcgctgg acgccgatga atctgcatca
     1561 ggcgcagaat cacgatgaaa aagtacgtat tgctaaagcg gcctcgcagc tggttaatcc
     1621 gggcgaaagc gtagtcatca actgcggctc caccgcgttt ctgcttgggc gggaaatgtg
     1681 tggcaagcca gtgcaaatca tcactaatta tctaccgctg gcaaattacc tgatcgatca
     1741 agaacatgac agcgtgatca ttatgggtgg acagtacaac aaaagtcagt ccatcacttt
     1801 aagcccgcag ggcagcgaaa acagtctcta tgccgggcac tggatgttta ccagcggaaa
     1861 agggctgacc gcagaagggt tgtataaaac cgatatgctg acagcaatgg cagagcagaa
     1921 gatgctgagc gtggtaggga aactggtggt actggttgat agcagtaaga ttggcgaacg
     1981 cgcgggaatg ctttttagcc gtgccgatca aatcgatatg ctgatcaccg gcaaaaatgc
     2041 taacccggaa atcctgcaac aactggaagc gcaaggggtc agcattctgc gtgtttaaag
     2101 atgctggcgg aaaaatgtca cggcagcatc taacgcctca ggcgtaatgc ggtgacgcac
     2161 gcctggctgc catgaacagg ttagcagttt atcccgtccc gtctcgctta aggcctgctg
     2221 caaacgtagt gattcgtcgg caggcacaac gtcatcgtcg aggccatgcc acagcagtag
     2281 aggtctgtcc ccaagttgtt ccaggtggtt tgtcgcttcc cactctgcca gcggcgcgac
     2341 aattttattg aattcattct gctgtgctgc cgtttcaggt atcagcggtg gaaacagtga
     2401 acgggcgagc gatgtaaaat agcccgatcc catcatgctg gcggtacatt tcaccgtggg
     2461 gtggcgagcg gtaattccca gtgccgtcat cgcgcccatc gacgcgccac cgactgccag
     2521 acggtcatca agcagccagt tttcttcggc tattgccgca cgtaaagtag tgaattcctg
     2581 catactttgt agcaagattt gccagaattg atttaaccgc cgcgcttcgt cgccactaaa
     2641 acggctaccg tgatcgggcg catccggcat gatcacccgc aaaccagcct gcgccagcgc
     2701 aacggcaaaa tagctataca ccaggctgga tgaagtaaag ccgtgataaa aaattacgca
     2761 cggtaacggg gtatcttttt gcccgacagg ataagcatga agaacgggaa tatctgccag
     2821 ctcgcgtgat tctatttcaa tcatctgtct ctcctttttg tctggtatga catgaagaaa
     2881 tccttagcac aagtttctgc tggcggtatt agttcataat gttgagatgt gggttacgct
     2941 ttcgtcacgt tttcattcga aaatcgcgta ccaggtaaca attcgggaac attcccccaa
     3001 agttaaatta ataaggcact acactatggt tagcaggaaa cgtaatagcg ttatttaccg
     3061 gtttgccagt ttattattgg tgttgatgtt aagtgcctgt agcgcactgc aaggtacgcc
     3121 acagccagca ccgccagtaa cggaccatcc gcaagagatt cgccgcgacc agacgcaagg
     3181 gttacagcga ataggtagcg taagcacgat ggttcggggt tctccggatg acgcattagc
     3241 agaaatcaga gcaaaagctg tcgctgcaaa agctgattat tacgttgtcg tcatggtgga
     3301 cgaaaccatc gtgacaggac agtggtattc acaagccatt ttgtaccgta aataaatcag
     3361 acaacttaca tagtcagctt tacattgctt tgcgtcccta tgcgtttacc tgtgcacaat
     3421 aaattaccgg ctggaagctt gggcgtttat taccgcgatg gaatgccctg caacatgtgg
     3481 ggaaacgaaa aatggagctg acgatgaaac aattacttgc ctcaccctcg ctgcaattag
     3541 tgacttatcc tgcgagcgcc acggcacagt ctgcagaatt cgccagtgct gactgcgtga
     3601 cgggcctgaa tgaaataggt cagatctcag ttagcaatat ttcaggggat ccgcaggacg
     3661 tggaacgtat cgtggcgtta aaggccgatg aacaaggtgc gtcatggtac cgcattatta
     3721 ccatgtacga agaccagcag cctgataact ggcgcgtaca ggcgatactg tatgcatgag
     3781 tgtcatctac agcaaatgaa caatgccgga tgcggcgcga gcgccttgtc cggcctacaa
     3841 ttttcacata tttcatttag ttaatcgaaa ccagcgtcgc atcagtcgta tacgcttaca
     3901 cacacactcc ccccgtcgcc cgcagcaata aatcattctg gatctgctct gacaaccgta
     3961 cgccgccgcg cgtatctaac atcacctgac accacgcctg cgccattggc ggagaagcat
     4021 atttcaacat ttgcgcaccg cagcccagca ggaatagctg atgagtaatc tctcgcccca
     4081 gttcttcagc tggcttacgc agctgctgct gtaaacgacg aaccgcgcga tcaaaatagc
     4141 gatcctgccc tttcacttcc acaaatgctt ccgacaataa gtcgtatacg cccgcttgct
     4201 tattgagaac gcgcaacaca tccaggcaca taatattgcc gg
//
LOCUS       DAZXUK010000290         4230 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZXUK010000290 DAZXUK010000000
VERSION     DAZXUK010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.290"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            345..3653
                     /gene="icsA"
                     /locus_tag="ACH3L8_003722"
                     /gene_synonym="virG"
     CDS             345..3653
                     /gene="icsA"
                     /locus_tag="ACH3L8_003722"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFT4086647.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            3797..4115
                     /locus_tag="ACH3L8_003723"
                     /pseudo
     CDS             3797..4115
                     /locus_tag="ACH3L8_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT         1279 a          729 c         1001 g         1221 t
ORIGIN      
        1 tattggcttt ggggattaac atcaaggggc agatagaacc attgggtgta tagaaaaaca
       61 aagtggttaa actttgggca agcgtacaga aagaactgaa aagttgcggt ctgaagcaga
      121 ctatcatagt aaaattatta atttattatc gaacatatag ctttccccct ctttttttca
      181 aagcaagaca caggtaaatt tctcccgttg cattgatata taacacagct ctcatgtttt
      241 ggttgaggct ttgtttaata tgtttttgca tatatcgtcc ctttattccg gataaaacgg
      301 aatcttttca ggggtttatc aaccacttac tgataatata gtgcatgaat caaattcaca
      361 aatttttttg taatatgacc caatgttcac aggggggggc cggagaatta cctacggtaa
      421 aggaaaaaac atgcaaattg tctttttctc cttttgttgt tggtgcatcc ctgttgctcg
      481 gggggccaat agcttttgct actcctcttt cgggtactca agaacttcat ttttcagagg
      541 acaattatga aaaattatta acacctgttg atggactttc tcccttggga gctggtgaag
      601 atggaatgga tgcgtggtat ataacttctt ccaacccctc tcatgcatct agaactaagc
      661 tacggattaa ctctgatatt atgattagcg caggtcatgg tggtgctggt gataataatg
      721 atggtaatag ttgtggcggt aatggtggtg actctattac cggatctgac ttgtctataa
      781 tcaatcaagg catgattctt ggtggtagcg gcggtagcgg tgctgaccat aacggtgatg
      841 gtggtgaggc tgttacagga gacaatctgt ttataataaa tggagaaatt atttcaggtg
      901 gacatggtgg cgatagttat agtgatagtg atggggggaa tggaggtgat gccgtcacag
      961 gagtcaattt acccataatc aacaaaggga ctatttccgg tggtaatgga ggtaacaatt
     1021 atggtgaggg tgatggcggt aatggaggtg atgccatcac aggaagcagc ctctctgtaa
     1081 tcaataaggg cacgttcgct ggaggcaacg gaggtgctgc ttacggttat ggttatgatg
     1141 gctacggtgg taatgctatc acaggagata acctgtctgt aatcaacaat ggagctattt
     1201 taggcggtaa tggtggacat tggggggatg ctataaatgg tagcaatatg accattgcta
     1261 atagcggata tataatttca ggtaaagaag atgatggaac acaaaatgta gcaggtaatg
     1321 ctatccacat cactggtgga aacaattcat taatactcca tgaaggttct gtcattactg
     1381 gtgatgtaca ggttaacaat tcatccattc tgaaaattat caacaatgat tacactggga
     1441 ccacaccaac tattgaaggt gatttatgtg ctggtgattg tacaactgtt tcactatcag
     1501 gtaacaaatt cactgtttca ggtgacgttt cttttggtga gaacagttct ttaaatttag
     1561 ctggaatcag tagtctggaa gcttctggaa atatgtcatt tggcaacaat gtaaaagtgg
     1621 aggctattat aaataactgg gcgcagaagg actataaact gctaagtgca gataaaggga
     1681 taacaggttt cagtgtttct aatatatcta tcatcaatcc gttactcact actggtgcta
     1741 ttgactatac aaaaagctat atcagtgacc agaataaatt gatctacggt ttgagctgga
     1801 atgatacaga tggcgacagt catggagagt tcaatctgaa agaaaacgct gaacttactg
     1861 ttagtactat tctggcagat aatctcagcc atcataatat aaatagctgg gacggaaaat
     1921 ccctaacaaa atcaggggag ggaactctca ttttggcgga aaaaaatacc tactctggtt
     1981 tcaccaacat caatgcaggc attctaaaaa tggggacagt tgaagctatg acacgtaccg
     2041 ctggtgttat tgttaataaa ggtgctacct tgaatttttc aggcatgaac caaactgtta
     2101 acactttatt aaatagtggg actgtgctaa tcaataatat taatgcccct tttttgcctg
     2161 accccgtcat tgtcacaggt aacatgactc tggagaaaaa cggtcatgtt attctcaata
     2221 atagttcgtc aaatgtcggt cagacctatg ttcagaaagg taattggcat ggaaagggcg
     2281 gaatattatc tttgggcgcg gttctcggca atgacaacag taaaactgac cggctggaaa
     2341 ttgcaggcca tgcgtctggt attacctatg ttgcagtgac aaatgaggga ggctctggag
     2401 ataaaactct tgaaggtgtt caaattattt cgacagattc ttctgataag aatgctttta
     2461 ttcagaaagg ccgtattgtt gctggtagtt atgactatcg cctgaaacag ggcactgtat
     2521 ctggactgaa taccaataag tggtatctaa ctagtcagat ggataatcaa gaatcaaaac
     2581 agatgagcaa tcaagagtct actcaaatga gtagtcgccg agctagttca cagcttgtat
     2641 cttcacttaa tttgggtgaa ggtagtattc acacatggcg ccctgaagct ggcagttata
     2701 ttgctaacct gatagcaatg aacacgatgt ttagtccttc tctctatgac cgacacggta
     2761 gcactattgt tgatcctact acaggtcagc tcagcgaaac caccatgtgg attcgtactg
     2821 ttggtggaca taatgagcat aatttagctg atagacaatt aaaaaccaca gctaacagga
     2881 tggtttatca gattggtgga gatattttga agacaaactt cactgatcat gatggcttgc
     2941 atgtgggtat tatgggagct tatggatatc aggatagcaa aactcataat aagtatacta
     3001 gttatagttc acgaggaact gtgagcggtt atactgccgg tttgtacagt tcttggtttc
     3061 aggatgaaaa agaacgaaca ggtctatata tggatgcttg gttgcagtac agttggttta
     3121 ataatacagt caaaggagat gggttaactg gtgagaaata ttccagcaaa ggaataacag
     3181 gagctttgga agctggctat atctacccaa ccatacgctg gactgctcat aataatattg
     3241 acaacgcatt gtatctcaat ccacaagtcc agataactag gcatggggta aaagcaaacg
     3301 actatattga acacaatggc actatggtca catcctctgg gggcaataat attcaagcaa
     3361 aattgggatt gcgtacatcc ttaattagtc agagttgtat cgataaggag actcttcgta
     3421 agttcgaacc atttttggaa gtgaattgga aatggagctc aaagcaatat ggtgtaatta
     3481 tgaatggcat gtcaaatcac cagataggca accgtaatgt gattgaactc aaaactggtg
     3541 tgggggggcg tcttgcagat aacctaagca tctggggaaa cgtatctcag caattgggta
     3601 ataacagtta cagagacacc caaggtattt tgggtgtgaa atataccttc tgataataaa
     3661 aatgcagggc gtgttgatgt cctgcatttc tctgggaccc accgtgtaaa agctcggtgg
     3721 gtttttgtgc acatgatggt atgcctgcga tcaatgccaa acgtgtttac cagatcatgc
     3781 gccagaatgc gctgttgctt ggcgttacac aggaattgca ttagaaagcg atgaacgaat
     3841 gctaccgcca aatattcgtg gttatgcgcc tcagattact ggtattgcag aaactaatgc
     3901 acgtgttgtt gtatctcaac aaggtcgagt aatatatgac tctactgtac cagctggaac
     3961 tttttctatt caggacttga gtagttctgt acgaggaata ttggatgttg aaatttttga
     4021 gcaaaatggt aaaagaaaac atttccaagt agaaatgtgc aggtgtgcct ttcttatcca
     4081 gacttggtca gagtaggtat aaattagtaa caggtctacc taaaacaaat aataaagcaa
     4141 ctggtgatgc atttttttcg gtgaagatcc tacggggtcc tgagcccggt gaagtggcga
     4201 ggcaaatcac atggggaagc gtacagcctg
//
LOCUS       DAZXUK010000291         4211 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZXUK010000291 DAZXUK010000000
VERSION     DAZXUK010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.291"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..437
                     /gene="tnpA"
                     /locus_tag="ACH3L8_003724"
                     /pseudo
     CDS             1..437
                     /gene="tnpA"
                     /locus_tag="ACH3L8_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(555..1493)
                     /gene="hypT"
                     /locus_tag="ACH3L8_003725"
                     /gene_synonym="qseD"
     CDS             complement(555..1493)
                     /gene="hypT"
                     /locus_tag="ACH3L8_003725"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFT4086648.1"
                     /translation="MKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKALEA
                     AIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAAAH
                     SLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQSPF
                     CCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGITG
                     RTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIYRM
                     NTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1844..2563
                     /locus_tag="ACH3L8_003726"
     CDS             1844..2563
                     /locus_tag="ACH3L8_003726"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFT4086649.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2629..3927
                     /locus_tag="ACH3L8_003727"
     CDS             2629..3927
                     /locus_tag="ACH3L8_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HFT4086650.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1060 a          913 c         1036 g         1202 t
ORIGIN      
        1 gcacacccga tggaactgta aatatcacat agttttttgc gccaaaatac cgaagacagg
       61 tgttctacag agagaagcgt agagcaacag gcagtatttt gagaaagctg tgtgagtgga
      121 aaagcgtacg gattctggaa gctgaatgct gtgcagatca tatccatatg cttgtggaga
      181 tcccgcccaa aatgagcgta tccggcttta tgggatatct gaaagggaaa agcagtctga
      241 tgctttacga gcagtttggt gatttgaaat tcaaatacag gaacagggag ttctggtgca
      301 ggggggacta cgtcgataca gtgggtaaga acacggcgaa gatacaggat tacataaagc
      361 accagcttga agaggataaa atgggagagc agttatcgat tccctgtccg ggcagcccgt
      421 ttacggaccg taagtaacga agttggatgc aaatgtcaga tcgtgtgcgc ctgttagggc
      481 gcggctggta agagagcctt ataggcgcat ttgaaaaacc tccggctatg ccggaggata
      541 tttattgcac gtcgtcaatt gcggcgtgct ggctcttcca ttgatatctg ctggattaaa
      601 tcgtctggca tatgttcctg taaaatacgc cagaggttct cggcggttct attcagtcgg
      661 gtgttcattc ggtaaatata agcctgaatg gggactgtca atttagcagc atcaaggcat
      721 atcagacgtt tggtctgtag ttcatcgacg atcgaccaga tcggtaacca ggcaatgccg
      781 tatccgttga gcgccatatt ctttaataac tcgctcatcg aagagataaa tatcgtgcgc
      841 ccggtaatac ctccaacctc tgccagatga cgattgacca acctgcccat ataagatgtg
      901 gcggtatagt tgagcaaagg aacctggggc tgagtgatat cgaatacagg gtgcccctgt
      961 gcgtcggcgg cacataccgg gtacaactca gattcaaaca gtttcaggca gcaaaaaggc
     1021 gattgcataa gatcttcatc gcgaaacgaa ataataaagt cactcttgcc ttcgcgtaac
     1081 gtatttacgg cctggacaac atcaatggct tcaacatgat agacaaattc tccgccatag
     1141 gcggtaagtg aatgcaccag tttgggtaat acgctcaggg atagtgagtg cgcggctgca
     1201 attttaatgt tgggaacgcc gagtaaactc tggccgttta actcccccaa attgcattca
     1261 agctgctgta ataagctgcg agtctgggaa tgaaagagct tgccctcttc ggttaattgt
     1321 aaaggggtgg tcgtgcgatc gaataaaaca acgccgatcg ctgcctccag cgccttaatt
     1381 cgtcggctga atgcgggttg cgaaagatta cgctcttttg ccgcctgaga aaaatggcga
     1441 caggcttcca gtgtcaggaa atcgtataac cattttgtct cgatattctt cattaccatg
     1501 gcacctgtat tttcaatcca aagacataat aaaacatccc ggcaataaaa ataatgttct
     1561 caattagcac aacctggcct ttatcattcg ttttgtgcat aaccccttca tttccggtat
     1621 tgacgttgat atcacatttc gccaaaaaaa cgaaattttt ccgtcagata accagaatac
     1681 atcatcacta tggtgaataa gcggaatgtt ccatagctgc atcatgcgtg tcttgcatcg
     1741 ccttgtacaa ccttgcattt ctcatcgccc atttcgactg ttaaaaaaga gagcagcagt
     1801 tatttaccgc gtattgtcat taacgttttg agaggcaggt attgtgaaag gtttaattgg
     1861 tgtactcggt ggaatggggc cggcagctac cgtcgacctg tttaataaat ttgtaacttt
     1921 taccgctgca caatgtgatc aggaacatat tcctttaatt atttcttcga tcccggatat
     1981 tcctgaccgt accgatgcat taatgcatca tgggcgttca ccattacccg ttatgcgtga
     2041 ttatatgcat aagctggaag atgccggggc agaatgtatt gttattccct gtaataccgc
     2101 gcattactgg ttcaaagaat taaaagatgc ttgtcatatt gatatattaa gtatcgtaga
     2161 aactacgatc aatgaagttc gcgcctgtgg taaaacccga attggcttgt tagccaccaa
     2221 tgccacgcta tatatgggtt tataccaaaa aggaattgag tcgctgggat ttacctgtgt
     2281 cagccccgat gccgacggtc agaaaaaagt gatggaaagt atttattcct tgaaagctgg
     2341 caatatagcg cacgcacaaa agttgatgaa tgagcaggct gaaatattat tttcccgcgg
     2401 tgcagaaatc cttgttcttg gttgtacaga aattccggtt attctggcgc aagccgttaa
     2461 agagcagcct tcccgctata ttgactcaac ggcgtcactc gttcgtgccg gaataaaatg
     2521 gtacgaaaat cgtgtcggta aacattatct tttaacccaa taatcattta aatcgcagcc
     2581 agaaagttat tcgcttttaa ctgaattata tttataacgg agaacattat ggtttggctg
     2641 gaaattatcg tagtacttgg tgcaatattt tttggtattc gccagggggg aatcggtatt
     2701 ggtttatgtg gcgggcttgg gcttgccatt ctgactctgg gatttggtct gcctatgggg
     2761 tcgccgccag tcgacgtgat cctgattatt atgaccgttg ttgtggcggc ctcagcctta
     2821 caggccgcag gcggaatgga ttatctggta cgcgtcgctg gtaattttat gcggcgtaac
     2881 ccgaaatata tcaacataat cgcgccgatt atcacctggt ctatgaccat tatggccggt
     2941 actgggttta ttgttttttc cacgctgccg gtgattgctg aagtggcgaa agagtccggt
     3001 atccgtcctt cccgcacgct tgccggttcg gttgtcgctt cgcaggttgc gatttccggt
     3061 tcgccgatca gcgccgcaat ggcggctatg ctcactatta tggaggtaaa cggtgtcagt
     3121 tttattcaag tgatgtcggt atgtcttccc acctcttttg ctgccgcgat ggtcgccgct
     3181 tttattgctt cccgacaggg atgtgagttg caggatgacg aggtctacct tgagcgcctg
     3241 caaaaaggac tggtgcaaaa atacgagaat aatagcagca tcaagccagg tgcgacgctc
     3301 tccgtcggat tgttcctgct ggcgacggtc gctattgtta ttcttgctgc cttcccccaa
     3361 ctgcgtccgg gttttgatat cggtaaaccg atggaaacac gcgatatcat tattatctgt
     3421 atgctctctg ccgcctgcct gatggtggtg ctgtgtaaga cggcgaccga tgccattatt
     3481 ctcacctcca cattccgtgc ggggatgagt tcgctggcgg taattctcgg cattgtaacc
     3541 ttaggcacca cttttattga tgcgcattta actgagatta aagatattgc tggtgatatt
     3601 ttgcaggcgt atccgatgtt gttggcgctg gttctttttg gcacttgcgc gctgctctac
     3661 tcgcaaggtg cgaccacgcc gttaattatc ccgcttgccg tcgcgcttga cgtgcccacc
     3721 tgggcaattc tggcgtccta tgtggccgtc accggagtat ttgtactgcc aacgtatcca
     3781 acatcactgg ctgccatgga atttgatact accggcacaa ctcgtgtagg caaatatgtg
     3841 ctgaatcatc catttatgct gcctgggttg gggggaatta ttgctggagt ggcattaggc
     3901 tttgtgattg cgccaatgat tatttagcac tatcaaacat cagtatcgat ggtttataca
     3961 caataaagac agatatggcg gaatatagtc tgttgtaagc gccaacaaag aataggggaa
     4021 ttccgccatg tctgaacatc ttcacaatat ggtattaagt gttgtcgttt aatgatttat
     4081 ttcttggtta tgttgtgtat tagctgcatc tatcgctttt tgatacctga ggtgtactgg
     4141 caatagcgga cactaccatt tgttcttttt ttaagcagtc atctgatgat atttttccct
     4201 gaaggctgcc g
//
LOCUS       DAZXUK010000292         4209 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZXUK010000292 DAZXUK010000000
VERSION     DAZXUK010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.292"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..209)
                     /locus_tag="ACH3L8_003728"
     CDS             complement(<1..209)
                     /locus_tag="ACH3L8_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFT4086651.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACH3L8_003729"
     tRNA            complement(324..399)
                     /locus_tag="ACH3L8_003729"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACH3L8_003730"
     tRNA            complement(406..480)
                     /locus_tag="ACH3L8_003730"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACH3L8_003731"
     tRNA            complement(597..681)
                     /locus_tag="ACH3L8_003731"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACH3L8_003732"
     tRNA            complement(690..765)
                     /locus_tag="ACH3L8_003732"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACH3L8_003733"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACH3L8_003733"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFT4086652.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACH3L8_003734"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACH3L8_003734"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFT4086653.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACH3L8_003735"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACH3L8_003735"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFT4086654.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAZXUK010000293         4197 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZXUK010000293 DAZXUK010000000
VERSION     DAZXUK010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.293"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            84..751
                     /gene="ykgH"
                     /locus_tag="ACH3L8_003736"
                     /pseudo
     CDS             84..751
                     /gene="ykgH"
                     /locus_tag="ACH3L8_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
     gene            complement(994..1689)
                     /locus_tag="ACH3L8_003737"
     CDS             complement(994..1689)
                     /locus_tag="ACH3L8_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFT4086655.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1682..3109)
                     /locus_tag="ACH3L8_003738"
     CDS             complement(1682..3109)
                     /locus_tag="ACH3L8_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFT4086656.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3120..3839)
                     /locus_tag="ACH3L8_003739"
     CDS             complement(3120..3839)
                     /locus_tag="ACH3L8_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFT4086657.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1118 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaatggt
      721 agatgaatac caacaaaact ctgggcagta ataaacagca ttctgataga gagtgcctgt
      781 ataaagctga agtgacaatg accgggaagg gaacccgttt tcaggaatgg atgcctgtct
      841 ttttcactgt aaatatcctg gcattactaa tgcctggata tttacagaag agcagcataa
      901 ccatgacgga acggggttaa gtcaggttgt ccttaacgct attttcaacc tcgtatgcct
      961 tcttcaggtt tatgttcaga tctcatatct ctctcagcaa tcctcaataa tcagatacac
     1021 cgctttcacc ggaccatgaa ccccgacgac tttgataagc tcaatatccg ccgttgagct
     1081 gggaccactg atgatgttaa tacatgacgg cattcgttca ccggcctgcg ctttctgatg
     1141 caatttttct gcgagttgcg ctacacgcgg caggattgtg cttttacgca ggataaaaag
     1201 agaagattcc gggagcaggc tcaatgaacg cccgcgctcg gcggcggaaa aaagaaccac
     1261 gcctcccgat tcggttaaac catattcagc atacacgaca cccactttag cctgctctgc
     1321 ctgcgagata ttctcggcac ctttcgccgg atcccaaaca acggcattgc attcctgctg
     1381 caaacgttca ctaatcccca attcctccag cctcgtgtca ccgctaatca cgaccgactg
     1441 atctcccagc tctttacaca gacgtattgc agcttctgcc gccttcgcct cgctggtcag
     1501 ctcacagcgc gtcaacataa catcgctggc aaactgaata aacgcgtcac agcgctgctg
     1561 ttggttaagt tgggtaagcc gctcgttagc atagttgtta agcggcgcat cttctgcttg
     1621 cggttcaagt cgcagcgggc gacccagtgc ctgagcaacg ttattcaaaa attcgctccg
     1681 attatccatt ctttttctcc tgcgcctgat gtttcttaaa ccaactacgg aaactctctc
     1741 cgtcagcttc aggaagatcg cgtgcttcca tccagtcgct aatcgcgcca aatttgagtg
     1801 gtgttttgcc gccattgata aaccagcttg ccgcatgagc gccggccatc atcccgactt
     1861 tccacaatcc tggatgacta ttggcataag cgaacatttt tatcgcccgt tgctctgctt
     1921 ttgcggtgat ccctttttca gccatcaccc gacgatgacg caaaatcagt tttgacagcg
     1981 gaatacgcac cggacacacg ctgtcacaag cagtgcataa agagcaggcg tagggtaaat
     2041 ctttaaaatc tttatagccg ccaagtagcg gagaaatcac cgcaccaatt ggccctggat
     2101 aaatagagcc atatccatga ccgccaatat ggcgatatgc cggacaagta ttcatacaag
     2161 ccccgcagcg aatacagcgc agcacatccc gaaattcaga ggccagcacc tcagaacgcc
     2221 cgttatcgac aataaccaga tgaaactctt caggaccatc aacgtgcccc gcttcgcgcg
     2281 gccctgtcag ccaggtgttg tatcccgtca aacgtgcacc aacggcactg cgcgccagca
     2341 tggtgatcaa tacatctacc tcggcaaacg tgggggcaat acgctccatt cccatcactg
     2401 caatatgcgt tttaggcagc gtggtacaca ttcgcgcatt accttcattg gtcaccaggc
     2461 ataccgaacc ggtctctgcc accgcgaaat tacagccggt aatacctatt tcagcactga
     2521 ggaaatcttc acggattttt tgccggatga ataaggtcat cgcttcaggc gtttccggcc
     2581 cctcatagcc cagacgttcg tgtagcactc gacggatctg atggcgatct ttatgaattg
     2641 ccgggaccac aacatgagat ggcggatctt gatccagctg gagaatatat tcacccagat
     2701 cggtttcaat cacctgaatg ccagcatcct gcaacacatg attgacacca atctcttcgg
     2761 tcaccatcga tttagatttc accaccttcc gggcattttt gcgttgggca acctgtaaaa
     2821 tgtagcgggt agcgtcttct ttggtttttg caaaatagac gtgaccgccg ttttgcgtca
     2881 ctttttctga gagctggtac agataagcgt cgagattact cagaacatga tcacgtatct
     2941 gggcggcccg atcgcgccac tcctcccagt gccccaattc atcgaccatt ttttgccgat
     3001 ttgccccaat acgctgctgc gcgcttgcca ccgctttgcg catgatcgga tcttcaattt
     3061 gctgacggat gcgtgtctta aaatctgtat tactggtttt gatcgacatc tttatatcct
     3121 cagcggctca tcaacacttc agcaatatgc atcactttga ctttctgccc ttcccgttgt
     3181 aatcgcccac tgatgttcag caggcaactc acgtcagcac caattaaata ctcagggcgg
     3241 acttccatca ggtgcacaac cttttctttc accatctcgc cggatatttc ggccattttg
     3301 accgagaacg tgccgccaaa tccgcagcag gtatcctgtt cagcaaaggt caacagctcc
     3361 agtccacgca catttttcag cagcgtaagt ggctcgtcct tcactcccag cttacgggcc
     3421 aggctacaag atgggtgata cacagctctc ccttgcaaac tggcacctac atcgactacc
     3481 cctaatttat taacaataaa agaggtgaga tcctgcatac gcgcggcaac cttttcggca
     3541 cgtgatgccc attcaggttc atccgccaga tacgtcgggt aactttttat ggcataggtg
     3601 caagagccag ccggtgaaat aatgggatcg tcgttatcct ccagtgcggc gatcagattt
     3661 ttcatccctg gaatcgcttc tttgatataa ccgctattga tcgcaggctg accgcagcat
     3721 ccctgtttct ccgggaaatt tacgcgacag ccgagttttt ccagtagcag cacggagtct
     3781 cgtgccattc ttgatttcag ggcgtcacca atacaggtga caaagaaatt gacattcaca
     3841 actaatactc cattacttca tgcccattta tgcccgatgt ttccccccca ccgggggatc
     3901 aatcatcaat aaatatggcg ttctttttta attggctagc aacataccac ccctcatttg
     3961 tatgacaaat gataatcttt catccaaact tcaaatgcaa taactaattt tatttctttc
     4021 ggcatcgctc acattttatg gggaaatcga cgatcatttt tggcataaga aatccattta
     4081 aaaatggcta catcccttat acctattcag agctacttat tcaggggtta ttgagatata
     4141 cactgatggt gtacaaaaaa atcaccgaca ataatcacgt atcaagtaat agtcgga
//
LOCUS       DAZXUK010000294         4182 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZXUK010000294 DAZXUK010000000
VERSION     DAZXUK010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.294"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..186)
                     /locus_tag="ACH3L8_003740"
     CDS             complement(<1..186)
                     /locus_tag="ACH3L8_003740"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFT4086658.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACH3L8_003741"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACH3L8_003741"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFT4086659.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="ACH3L8_003742"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="ACH3L8_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFT4086660.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="ACH3L8_003743"
     CDS             3863..4144
                     /locus_tag="ACH3L8_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFT4086661.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1044 c         1062 g         1060 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagc
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAZXUK010000295         4168 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZXUK010000295 DAZXUK010000000
VERSION     DAZXUK010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.295"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACH3L8_003744"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACH3L8_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFT4086662.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACH3L8_003745"
                     /pseudo
     CDS             complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACH3L8_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACH3L8_003746"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACH3L8_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            3787..>4168
                     /locus_tag="ACH3L8_003747"
     CDS             3787..>4168
                     /locus_tag="ACH3L8_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFT4086663.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1038 a         1208 c         1021 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAZXUK010000296         4168 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZXUK010000296 DAZXUK010000000
VERSION     DAZXUK010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.296"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(82..1941)
                     /gene="gss"
                     /locus_tag="ACH3L8_003748"
     CDS             complement(82..1941)
                     /gene="gss"
                     /locus_tag="ACH3L8_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFT4086664.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2146..3012
                     /gene="yghU"
                     /locus_tag="ACH3L8_003749"
     CDS             2146..3012
                     /gene="yghU"
                     /locus_tag="ACH3L8_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFT4086665.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3041..3122)
                     /locus_tag="ACH3L8_003750"
                     /pseudo
     CDS             complement(3041..3122)
                     /locus_tag="ACH3L8_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3135..3383)
                     /gene="hybG"
                     /locus_tag="ACH3L8_003751"
     CDS             complement(3135..3383)
                     /gene="hybG"
                     /locus_tag="ACH3L8_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFT4086666.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3517..3858)
                     /gene="hypA"
                     /locus_tag="ACH3L8_003752"
     CDS             complement(3517..3858)
                     /gene="hypA"
                     /locus_tag="ACH3L8_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFT4086667.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3851..>4168)
                     /gene="hybE"
                     /locus_tag="ACH3L8_003753"
     CDS             complement(3851..>4168)
                     /gene="hybE"
                     /locus_tag="ACH3L8_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[NiFe]-hydrogenase assembly chaperone HybE"
                     /protein_id="HFT4086668.1"
                     /translation="TPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDG
                     VSQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRG
                     ENA"
BASE COUNT          977 a         1150 c         1052 g          989 t
ORIGIN      
        1 gaatatataa atattgaata ttttgattaa tccccgtact aattattctt cataatcagt
       61 acggggttgc tacaacatgt attacttttt caccacaatt aacggttcaa tatcactctc
      121 ttttttgatg accagtgatt catcaccgcg caaacacgtc ccaccgtagt tgccgccaac
      181 ggtgaaggta catacctgaa tgtatttacc gtccactttc ggcaaacacc acagttgctg
      241 atagatgttt ttctgctcgg caaatttacc gctggttttg tccagcacct cttcatgatg
      301 gctgaccagg tcgatattgc taccacagcg accggcgatc ggtttcactg cgtaacctgt
      361 tttcaccaat tcatcattaa cagtgaaatc ggtatccagc aggtaacgat ggtgcgggaa
      421 cagcgaccag aggatcggca gaatcgcttt gttgccgggg atcaccgtcc acagcggctc
      481 aaagaccagc acttccgggc gcagcaatac gtcgataaga cgcacttcgt tttgcggatg
      541 accggtacgg attggcaccg cagcaaactc acggtcgcta acttcacgaa tctgatcaaa
      601 cgcggtttcc cacgcccagg ttttccacac gcagttaacc agtcgccctt ccccatcaat
      661 cagttgcccg gcagcatccc agcccagttc atccagcccg cgcaagatac gcgtttcaaa
      721 gcccgcctgg tgcagcgcct gctccataaa ctgcgcgtga tagttttcct cgatatcttt
      781 gtcctgcatg atatggacaa acggacgtgc acgactgtgt ttccaggcac cagccagttc
      841 gttaatcagc ccttccgccg gattgaagcc gttgccttta tagccctgct ccgcccaacg
      901 ttcgaggatc aagcccgctt cggtatgaca ggaggcggag tcggcgttgt attcgtaaac
      961 cttcaggcca cgctcatcca tgcagaaatc catacgacca gtgatcatat ggtgacggcg
     1021 acgctgccag gagagacgca aacgtggcca gaggattttc ggaatgtcga acagcgccag
     1081 caggttgtca tctttcagca ccttgtcggt tgcgtgaaga tacatcagat gcagctcgtt
     1141 ggtggcttta attagctcct gctcggcact ctcggtaatg gtgtagtaat gatacggatc
     1201 ctgattgatc acctgaccgt tggcctgcac ataggcgttt tgtagcggat ctttttcatc
     1261 cagccattta ccgtcaaact ggcctttgtt ttccagacgc gctccactga ttttcagcag
     1321 ctcgcctgca atttccggct gcggtaagct gtattcagta tcttccgtct ggatcatcca
     1381 gcccagaatg gtggtgtcat caaaagtgtc tttaagggta tagcagccgt tttcgaccac
     1441 catctccagc tcgcgcgtcc actgttgccc ttgcggcaac ggggaatgaa tcacgttctg
     1501 ttccgcaata cggactttgt tgccatgcaa ttgggtaatg atggcgacat ggccagtgtc
     1561 tttaaattca ccgcctttat cccagataag aagcgcaccc gcgaccggcg cacgcggcga
     1621 gccgttagga aatgcctgca atggcaggat gttgtcatta accacttctc gcaggaagcg
     1681 cagcgagaaa atctcccacg ccatacccac gtcagtaaag accacaccgt aattcagaaa
     1741 gagaaaacgg cgagcaaatt caacgcattg ccacttgtgg cccatatatt cgtcgtcgat
     1801 atagctacgg aatacggcgt catcttcgta ttcctgcgga tcgagagaac tgtaatctga
     1861 agagtagatt gctaccccac ctggggcgta gcccaataat gtcccgaacg gggcatcctg
     1921 gctggtcgtt cctttgctca tcactttacc tttaacaata caacctaagc agttggcgta
     1981 attgttgtgc tctgattacc tgctcagcga cattaaccgg acagaggtca gactaatcat
     2041 acacctcatc gcagcggctg gcgcaaaggg ttaaaaaatt cacattctgt acagcaagtg
     2101 acctgctaca ctgcttcaac actaccactc agaaggcaac tcactatgac agacaatact
     2161 tatcagcccg cgaaagtctg gacgtgggat aaatccgctg gcggcgcgtt cgccaatatc
     2221 aatcgcccgt tttctggtcc gacgcatgaa aaaacgctgc ccgttggcaa acatccattg
     2281 caactttatt cgctgggaac gccgaacggt cagaaagtaa cgattatgct tgaggagcta
     2341 ctggcgctgg gcgttactgg tgcagagtac gacgcctggc tgattcgtat tggcgatggc
     2401 gatcaattct ccagcggctt tgtcgaagtg aacccaaact cgaagatccc ggcgctgcgc
     2461 gatcatacgc ataatccgcc gatccgcgtg tttgaatctg gttcgatcct gctttatctg
     2521 gcggagaaat ttggctactt cctgccgcag gatctggcaa aacggactga aacgttgaac
     2581 tggctgttct ggttacaggg tgccgcgccg ttcctcggcg gtggttttgg tcacttttac
     2641 cattacgcgc cggtgaaaat tgagtacgcc atcaaccgct ttaccatgga agccaagcgc
     2701 ctgctcgacg tgctggataa gcaactggcg cagcataagt ttgttgcggg cgatgagtac
     2761 accattgcgg atatggcgat ttggccgtgg tttggcaacg tggtgctcgg cggcgtgtat
     2821 gatgccgcag agtttctgga tgcgggaagt tataaacatg tgcaacgctg ggcgaaagaa
     2881 gtgggcgaac gtccggcagt gaaacgtggg cgtattgtta accgcaccaa cgggccgctg
     2941 aacgagcagt tgcatgagcg ccatgacgcc agtgattttg agacaaatac agaagataag
     3001 cgtcaggggt aagggttggt gttcgtcgca gcaagccatc caggccggat aaggcgttca
     3061 cgccgcatcc ggcaatcgtg cacaatgcct gatgcgacgc tgccgcgtct tatcaggcct
     3121 acaaaagcta atcatcacgc gctggtaatg tcgtaatcca tttggcgcag tgcgtctaat
     3181 gtggctttgg cttcatcttc gtcgatgatg ctcatggcaa atccgacgtg taccagcacc
     3241 cactggccca gcagatcggc agggttacct tcgcaaatca gggcgatatt cacgtcgcgc
     3301 ttgataccac atacttcaac ctgcgcaagc tggtgaatat cttcaccgac agccagcacc
     3361 tggcctggaa cgccaataca cattgttaac tccggttatt tcgattgccg gacaaacgcg
     3421 aaaaagaacc tttagcgggt gttccctctc accctaaccc tctccccgga ggggcgaggg
     3481 gaccgtccgt gcgcttttta ggcgttgtcc tcagtcttat tcaacttcaa tacttttgac
     3541 gatcagcgaa tcgccggtat cgacacgcaa ccgctcgccg tgacagagcg gacactgcgc
     3601 atcgtgctga tgaatctcca ccacctggct gcaatcccag caccaggcct gggcgggttt
     3661 atagacgata tgtaaatcgc acccttgcgc caccgttccg tggcagacaa tttcaaaact
     3721 aaaacggacg gcgctctcct caacgcagga gagcgcgcca atttccagcc acacggcggt
     3781 gacgcgctta acatcgtgct gctccgcctg ccgttggata atttcaacgg cgctctggca
     3841 aagcgacaac tcatgcattt tcgcccctcc tgcggccaaa cagcagggcg cgacgccctg
     3901 cgtgtggtac atccggattg gtgactggca acgaaaggat cattcgtgcg cagtcatccg
     3961 tcaggcgttg gccttcttca atcgacatgc tgtgcgaaag cggcgacatc agcgaacagg
     4021 agaggtattg cgaaacaccg tccagttcgc caacggtaaa ggtcatggtg ccatacggca
     4081 gttgcagacc aattttttca ctcactttgc gcagcggcca gagttgatcc gggccgggga
     4141 agataactgc actcagcatc cacggggt
//
LOCUS       DAZXUK010000297         4088 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZXUK010000297 DAZXUK010000000
VERSION     DAZXUK010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.297"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(34..360)
                     /locus_tag="ACH3L8_003754"
                     /pseudo
     CDS             complement(34..360)
                     /locus_tag="ACH3L8_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(370..918)
                     /locus_tag="ACH3L8_003755"
     CDS             complement(370..918)
                     /locus_tag="ACH3L8_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFT4086669.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(918..1625)
                     /locus_tag="ACH3L8_003756"
     CDS             complement(918..1625)
                     /locus_tag="ACH3L8_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFT4086670.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1640..2317)
                     /gene="ydjY"
                     /locus_tag="ACH3L8_003757"
     CDS             complement(1640..2317)
                     /gene="ydjY"
                     /locus_tag="ACH3L8_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFT4086671.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2322..3032)
                     /locus_tag="ACH3L8_003758"
     CDS             complement(2322..3032)
                     /locus_tag="ACH3L8_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFT4086672.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3199..4005)
                     /gene="xthA"
                     /locus_tag="ACH3L8_003759"
     CDS             complement(3199..4005)
                     /gene="xthA"
                     /locus_tag="ACH3L8_003759"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFT4086673.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         1040 a         1052 c         1039 g          957 t
ORIGIN      
        1 aatgccagtc agttgcttaa ctgactggca ttactctttt aaggtgcgga agttttcgcc
       61 gttcacccag agcagatcca ccgagccgcc cgttttacga ccggcagcag cttcggtctg
      121 aatgcgcttc accgcgtctg cggcatccgc gagacgaaca atcttcaggt ttatagcgta
      181 atgggttttc atctcgccgc taacccagtc gagatagcgg ttaattgcgg tatcgccgcc
      241 ccaggcgtta aaccagacgg tttgcccttt ggcctcattt ttaatttcct gccagtcact
      301 ggcatgtgtt gccaaagaaa acagcgataa caatcccagc aaccacccac aatggcgcat
      361 cttccctcct taagaacgtt tttccggaat acggcacagc ccctgagcgg gaatgtccat
      421 ggcagaatta aaacgagccg acaggttttg caggccgatc aatgccgtca gttcggtgag
      481 cgcctgagcg tcaaaatgcg cagccagtcg ggtacgcagg gcatcatcga ctgttggcgg
      541 tgtgacgctt gcggcttcgg cgtactccag cgccagccgt tcttcatcgc taaagagcgg
      601 gctttgccgc cagtcagcca ctgccagcag tttatcggtg ctgccggtac gctcggcgac
      661 tttcatactg gtgatatcca cgcaaaactc acacaggcac atttgcgcaa tgcgcgcgct
      721 gacaagcgat cgcactaccg gatcgagcgg tgagcgtttg cgctccagcc acccaacaaa
      781 cattgacacc aaataaaaga taaacgggat ccgcccccac cagcgaatcg ggcttagcac
      841 ctcgccgtaa tggcgacgat gcagccacgc ctgcggacgt aaaatgaaag gaattttgct
      901 aagcggcggt aaacccatca gtcattcctc ttctggcgct caagccatat tttcttcgcc
      961 ataaaaatca ccacggtcag ggcaaacaga ataaacagtt cggtgacaaa ccagaaagta
     1021 ccgcctgtta acatgctgcc tgcccaggaa taaacaatag tcgccggtaa ttgaccaagc
     1081 ccggtggcga taaaaaacga gcgaaaacgt attgaagtca gaccggcagc atagctgatt
     1141 ggatcgaaag ggacaaaagg caataaccga cagaccagga tggtgtgttt gccgtagcga
     1201 gtgaaaaagc cgtccatact gtcaagcacg gttttgccgg ttaatttttc caccacttcg
     1261 cggcccatca ctctggcgat aaaaaagcac agcgccgcgc cggccatcga actggtccac
     1321 gacagcaagc cgccccagaa cgcgccaaac agcgacgcat tggcaaaggt gatcaaaaac
     1381 gcaggtagcg gtgcagcaat ggcctgtaaa atcatcaaca agaacgagac aactgccgcc
     1441 agtgcgccgt aagactgaat aaagcgttct ataccctgtt ggtccacggc ggcaaacgcc
     1501 gcaacgctgc gattgataaa ctcatggacg ccgggaagca gcgcccatgc gagcagcata
     1561 gcgaacaaca ggatgatgag ggttatacgg tagtaccaga ttttacgcga ttgtatcttc
     1621 atcatccttt atcttggctt tattcggtga ttttaaaggt aaccgttgcc agcgtgttat
     1681 ccgccgggag aactgaggca ttgcctttga atttcacttc gccgtgtttt tccaccgcac
     1741 cataagtgta tgttgcattg ctgacgatgc cgaccgggca gctatccaga cacaccaggc
     1801 aacccgtttt cttctcttct gctgccgtta aattaccgcc aaagcgcatg tccagtttct
     1861 tgccattact gtcaacaatc acttcatcga aggaatacgc ttttgccgcc ccttgccagt
     1921 tgaccgaaat atccagtttg ctgcctgtga catgagtcgt ttctttatta tccatcgtca
     1981 tgttttcgcc cggcgtacca cctgcctctt tcagggcttc ataaaatgct ttcgggggcg
     2041 cataagccat aaacagcgat ttatgtccgt tggagccatc tttaaacaca ataccgtgac
     2101 gagtgtcgtc ggtgaggaaa cgaccattaa tttgcaccag catggttacc gtatggttat
     2161 tggcatcgac gcgcagcggt tggctgttcg aaagtccgtc gtattccact tttgccgccg
     2221 cattctcttt ttgatcgcag ccgctaagcc cggcaacagc cagtaagcag agtgcagcca
     2281 gtcctttttt ccatgacact gaataatgtt gcgacatcgt tctatccttc attttttggg
     2341 tgagtaagtg ggctgcggcg cgaagcagac agatccacat gtttgtgtcg ggcgtagagt
     2401 tttgcgagct ggatgagaat aaacagcgcc aggcccgcca gacagagttg taaaataaag
     2461 cgcagcgtaa tgccttcatt ggcgagatcg cttgccatca cggtataaat aacaataccg
     2521 ggcagggtcg tgagtgccga aataagggta taaggccaga aggcgatggt ggtgaatccg
     2581 taagcgtaat tttgaatatt gtaaggaaac aacgggatta agcgggtcag aataagaaaa
     2641 tcaataccgt tacgcgcaat gcctttttca atggcctgga aggtatggct atggcccacg
     2701 tatttcagca gtaaatcacg tcccagccag cgcgccagca ggaatgagca cgaggaggcc
     2761 agcgttgcgg caattaatga gagttgtgtc cctaagagcg ggccaaaaac tattcctcca
     2821 gcgatcacca gtatgcttcc tggtaacaga aagagggggg cattgatgaa taacagaata
     2881 tagaggctat aaccgaacaa tccgctctgg cgaatgagtg tctgtaagtg gggtaaatcg
     2941 gtgagcagat cgaataaacc gaaagcgtgg atagcgtaaa tgaccagcgc aaaaataaga
     3001 caggcaaaca gaaagttacg ctcagcgttc atcgtggagt ttcctatatc aaacttgata
     3061 atagagaagt ggtcagacaa ctaaaaacgc agtgacggta taacaaagga cggcaggcaa
     3121 caaatcatta ctgacagttt gttgattttt ctcaaggtta attctcctga cccagtttga
     3181 gccaggagag ctgctaaatt agcggcggaa ggtcgcccag acgggggcgt gatcggacgg
     3241 tttttccatg ctgcgaattt catagtcgat gccggtttct acgcaacatt ctgccagcgg
     3301 ttggctggcg agcagcaggt cgatgcgcag accacggtta tcgtcaaaac cttttgagcg
     3361 gtaatcaaac catgagaaac gatctgctgt ttgcggatta gcatggcgga aggtatcgac
     3421 caccccccag ctcatcagcc tgtccatcca ttcgcgctct tccggcagga aagagcattt
     3481 accggtacgc agccagcgct tacggttttc ttcgccaatg ccgatatcca gatctgtagg
     3541 gctgatattc atatcgccca taatcagtac cggattatca cgtttgagtt cggtttccag
     3601 gtagttttgc agattctgat aaaactgcgc ttttgccggg aattttatcg gatggtcgcg
     3661 gctttcaccc tgcgggaagt aaccgttgat cacggtgaca ttacccagca gtgaggggat
     3721 ttccgccata ataatccgcc gctgcgcctc ttcgtcgtca ccgggaaagc cgcgacgcac
     3781 ggcaatcggc gtctctttgg tcagcagcgc cacgccataa tggcctttct gcccgtgata
     3841 aaacacgttg tagccgagct tcgccacctc ttcgagcgga aacatatcgt catgaacttt
     3901 tgtctcctgc aggccaatca catccggttg gtgcttttcg acgatggctt caagctggtg
     3961 aggtctggcg cgcaggccgt tgatattaaa agagacaaat ttcatagtcg ctgccattta
     4021 ttcagataaa gagtgcgtgg atggtagcag aatttcgcgt tgcttaccgc ctgttgttaa
     4081 gtgatttg
//
LOCUS       DAZXUK010000298         4073 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZXUK010000298 DAZXUK010000000
VERSION     DAZXUK010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.298"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            84..791
                     /locus_tag="ACH3L8_003760"
     CDS             84..791
                     /locus_tag="ACH3L8_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFT4086674.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            788..2239
                     /gene="djlC"
                     /locus_tag="ACH3L8_003761"
     CDS             788..2239
                     /gene="djlC"
                     /locus_tag="ACH3L8_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFT4086675.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2299..3969)
                     /gene="hscC"
                     /locus_tag="ACH3L8_003762"
                     /pseudo
     CDS             complement(2299..3969)
                     /gene="hscC"
                     /locus_tag="ACH3L8_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
BASE COUNT         1067 a          950 c         1009 g         1047 t
ORIGIN      
        1 tctttttatt ttgaaagcgt caagtttata ctcatcaata ataaaaaagt agtcagttaa
       61 tgcacttaag ggattattaa aaaatgaata aggaagaaca atatcttctt tttgcgcttt
      121 ctgcgcccat ggaaatactt aatcagagtt gcaaacccgc gcatgactcg ccaaaaatgt
      181 ataccgggat aaaagaattc gatctctcat catcctgggg aataaataat cgtgatgatt
      241 taattcagac tatttatcag atgactgatg atggtcatgc caatgattta gctggcttat
      301 atctgacatg gcaacgctct tcccctgaag agtggaaagc attaattgct ggtggctcgg
      361 agagagggtt gatttatacc caattcgttg cccaaaccgc tatgtgctgt ggagaagggg
      421 gaatcaaagc ctgggattat gttcgaatgg gatttctgtc acgggccggt gtgctcaaca
      481 actggctgac ggaagaagag agtttatggc tgcaatcgcg cgtctatgca cgagctcacc
      541 attactatca cagctggatg cactattttt ctgcctattc actagggcgt ctctactggc
      601 aatcttctca gtgcgaagac aacgcttcgc tacgcgaggc gctaaccctg tacaaatacg
      661 atagcgccgg gagtcggatg ttcgaagagc tggcagcggg aagcgaccgt ttctatgcca
      721 cgcttccctg gcagccatta actgtccagc ctgaatgccc ggtaacgctt aaggatgtga
      781 gcgacctatg aaaacatgtt ggcaaatcct cgaaattgaa agcacgacgc aaatagacat
      841 tatccgccag gcttatcttg ctcgcttacc gttgtgtcat cccgaaaccg atccgcaggg
      901 gtttaaagca ttacgccagg cctatgaaga ggcgctgcga ctggcggtaa atcctgtcga
      961 ggaagcagat gatgaagaaa aagatgccac cgctgaacat gaaatactac gtgcattcag
     1021 gacattactg gattcagaaa gtgatcgttt tcagccctcc gcctggcaga aatttattca
     1081 gcaattaaat acctggaaca tggaggatgt cgatcaatta cgctggccgc tgtgtgcaat
     1141 cgctatagaa gcgcgatatc tgtcattaaa ttgtgcttct ttgttggcag agcgtttgaa
     1201 ctggcattcg tttaatgaca gcgaaggaat ggatgaggaa gaaagggagg cttttcttga
     1261 ggccattcag gctggtgatt gtttcgattt ccttagcctt ctggaatatc ccgtcgcgtt
     1321 acagaaccag actgttgaat attacttcgc gctggaacgt tgctgccgtt accatcctga
     1381 ctatgtcact gcgtttttgg cgatggaagg accgtggttc attcctgatg atgcaaagtt
     1441 acatcgcaaa ctgttgcgct ggtacagctc ggtgcaaaca ggtatggtgg aactcattcc
     1501 tgtagccaaa cagtggcaag cggaagaacc agaaagcgaa gatgcccggt attaccagtg
     1561 tgcacaacgt ttgtactgcg gcgaggggga aagcctgctt gctgatctcg gcgcgtactg
     1621 ggaaagttac ccatctacac aagctgataa tttgttgttg cagtggagca agcgtcattg
     1681 cccggattat ttcgcgttat tagtgatggt tatcgaagcg cggagcatgg tagatgcgca
     1741 aggtcaaccg ctgaaatatg ttcctggtga gagcgcccgg acgcggctgt tatgggcgga
     1801 aattttacat agcggaaaat tatcgccgtt aggtcaatcg tttattgagt cgttattctt
     1861 caaacgcaaa gcatgggcgt ggtggaaatc gagagtcggt agcgagacag aagaagattc
     1921 accgttactg gatttgtatc gggtagcgga acaggtagta cttgaagcgt ttccgaaaca
     1981 agagatgctg gcccgtctta atacaaggct ggaaggcgga gatgctcatc cattagaggc
     2041 cattatcacc cggatgctgt tgacgaaagt aaaactcgag ccggaggatg aagatgtcga
     2101 tgagccaaca cctgaaaacc atgaagaaaa aaatgatgag ggtgaaaaac cacagagcat
     2161 taccagcatt atcaaaatca gtttaacggt gctggttata ggttatgctc tcggcaaaat
     2221 cgcgatgttg tttagttgac gatgtgtttt ttgcgccgat gccggatgca gcatttacgc
     2281 cgcatccggc agccgtgatt atgaggtctc aatggctaaa taaacgctcg cccgccgcct
     2341 gacctcatcg acgcgggcca tatcgtttga ctgcagcgcc gcatcaaaat cggtgatcac
     2401 ccggccaatc tcttctcgct cgtcacccag cgcccgcgcc cacaactctt ccagtttggc
     2461 tttaaaggtg cgattgatga gcatatcgcg cggataaatt ttcaatgcgg ataaccgcgt
     2521 ccgactctct tcaatctgct gcgcgctcaa tgttaccggg ctgtggttaa tcactctgga
     2581 cttaacgctg ccgtcttcca gaagcacgtc aacttcaagc aacccgttga tatcataact
     2641 aaagcgaata tcaatcgact gataagcccc ggttttcttc aacggcacat cgaaggattc
     2701 caccagaatg ttgtttttaa ctttgtggtt ttctccctga tagacgttaa ccgtaattga
     2761 atcctgttcc gggtgcatgg ttgaataagt ttctacacgc gacacgggca cagtggtgtt
     2821 tcgttcaata atcggcgaga aaatgccgga aacgccctgg cggttaacct caacgcccaa
     2881 cgagtaaggg caaatatcag tgaggatcac ttcttcaata tcttcactgc gtaagcggca
     2941 ggcggcctgg attgctgcgc ccagcgcgac aatggtgctc ggatcgtaac tttgatacgg
     3001 taatttgcca aacagacgca cggcgattcg ctgcaccagc ggcatttgtg acgcgccgcc
     3061 aaccagcacc agactgtcga tttgactcgg cttcagacgc gcatcgcgca acgcctgttc
     3121 aatcggcact cgcaagcgat tgagcagcgg caaccacaaa tcttccagtt cgttctcgta
     3181 aaattcgcat tcccgcgttt catcctggta ctaccagcga atgtgcattg gcgattgatt
     3241 gctacatttt gccgcttcca cacaggcgta caaggctgcc agttcactct cgttaagcgt
     3301 ggtccttgtg acatccgcgc gctttaaaac gtcatcgacc aacatatggg taaaatcttc
     3361 gccaccgagg aagttgtcgc cagcggaggc gtgaacttca atcaccggcg tggcgtactc
     3421 aagcaccgta acgtcaaacg tgccgccacc gagatcaaac accagcgaac gggtattttg
     3481 ttgggtatgc aggccatacg ccatcgcagc tgctgtgggt tcattaatta agcgtaccgc
     3541 atttaacccg gctaagtccg ctgctaaacg ggtatgcttg cgttgttcat cgctgaaata
     3601 agccagaacg gagatcacca catctttaat cggacgttgc agaaattctt cggcatcttc
     3661 ttttaatgag cgtaatacca aagaggacag ttctggcgcg ttaaatgtgt cgctgcctaa
     3721 ccgccagttg gtattactgc ccattgcacg tttaaataac gctgccgttt tatccggatg
     3781 cgaagtacgc cgtgatacag ccggttttcc gactaaaata tgattatttt catccatgct
     3841 aattatggat ggtgttaaat attcaccgaa cttatttgga attaattgcg cggcaccgtc
     3901 tttccagacg gcaattaaac tattggtagt gccgagatcg atacctatgg cgagttctgc
     3961 attatccatt gcatttatat cttgaaagaa aaagagataa atagattaag accacccagg
     4021 taacgcgtgg tgatcttaat caatgacgag tgttaagcgt aaaatttcag acg
//
LOCUS       DAZXUK010000299         4045 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZXUK010000299 DAZXUK010000000
VERSION     DAZXUK010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.299"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..975)
                     /gene="uidB"
                     /locus_tag="ACH3L8_003763"
                     /pseudo
     CDS             complement(1..975)
                     /gene="uidB"
                     /locus_tag="ACH3L8_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(981..2684)
                     /gene="uidA"
                     /locus_tag="ACH3L8_003764"
     CDS             complement(981..2684)
                     /gene="uidA"
                     /locus_tag="ACH3L8_003764"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFT4086676.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLSIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            complement(3072..3662)
                     /gene="uidR"
                     /locus_tag="ACH3L8_003765"
     CDS             complement(3072..3662)
                     /gene="uidR"
                     /locus_tag="ACH3L8_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFT4086677.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(3891..>4045)
                     /locus_tag="ACH3L8_003766"
     CDS             complement(3891..>4045)
                     /locus_tag="ACH3L8_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFT4086678.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
BASE COUNT         1045 a         1066 c          954 g          980 t
ORIGIN      
        1 aatttcaacc acgatttctt tgaatttttt atccgtgagc ggataaaacc agataataac
       61 gaatgccagt agcataaatc cgcaaggtac taaggcaatt gatgtgcgga tgcccataat
      121 aacttccggc gtttacacct gattggcgat atatccgctt aatcccaaaa taaaggcagg
      181 aattgaacct ccgattgcct gaccgcattt acgggtaaat gagaatagtg aataggtgag
      241 cccttcaatt cgcacgccgg tcaggtattc accgtactct acggtatcag cttccagtgc
      301 ccacatcaca gtcatagtaa cgccctgacc aattgaagcg atggccaacg caaccaacgc
      361 caccggcagt gaccagacgg aaacccagaa gaatagcaga taaccgcagg ttcccagcaa
      421 agcgccaatc aggaaggtat tctttttacc gatcctcgcg accatccccg gcacaagcgg
      481 tgccgatgcc acagtaccaa ccaggttttg caccagtacc agcacagtga acagcccggt
      541 atcatttaac acatagcgca cgtagaacaa cgacgaggcg ctgaccgcga aggtcgaaat
      601 cagcacacac agcgcaccga tgcacaacat aaacagcggg cgattccgtt tcagggtttg
      661 caaactgatc ttcaatgacg gctgcgccac gatacgtacc acattttcac gcgtcgattt
      721 gaagcagatg aagtaaagca ccattccggc aatcaccagc acaatcgtcc agaaatggta
      781 taccgacacc atctcttccg ggctggagtt cttaatgctc ggtcctatca gaaatgccag
      841 gcatacaaag gtcaatgaag cggcaatccc acgagccgcg cccagacggg cgcgagattg
      901 tggttgttgg gtcatcgcgg tagcaagtga accataagga atattcacca ggctgtagca
      961 aagcccgagg cccatgtagg tcaattgtta ccgccaacgc gcaatatgcc ttgcgaggtc
     1021 gcaaaatcgg cgaaattcca tacctgttca ccgacgacgg cgctgacgcg atcaaagacg
     1081 cggtgataca tatccagcca tgcacactga tactcttcac tccacatgtc ggtgtacatt
     1141 gagtgcaggc cggctaacgt atccacgccg tattcggtga tgataatcgg ctgatgcagt
     1201 ttctcctgcc aggccagcag ttctttttcc agtaccttct ctgccgtttc caaatcgccg
     1261 ctttggacat accaaccgta ataacggttc aggcacagca catcaaagag atcgctgatg
     1321 gtatcggtgt gagcgtcgca gaacattaca ttgacgcagg tgatcggacg cgtcgggtcg
     1381 agtttacgcg ttgcttccgc cagtggcgcg aaatattccc gtgcaccttg cggacgggta
     1441 tccggttcgt tggcaatact ccacatcacc acgcttgggt ggtttttgtc acgcgctatc
     1501 agctctttaa tcgcctgtaa gtgcgcctgc tgagtttccc cgttgacagc ctcttcgctg
     1561 tacagttctt tcggcttgtt gcccgcttcg aaaccaatgc ttaaagagag gttaaagccg
     1621 acagctgcag tttcatcaat caccacgatg ccatgttcat ctgcccagtc gagcatctct
     1681 tcagcgtaag ggtaatgcga ggtacggtag gagttggccc caatccagtc cattaatgcg
     1741 tgatcgtgca ccatcagcac gttatcgaat cctttgcctc gcaaatccgc atcttcatga
     1801 cgaccaaagc cagtaaagta gaacggtttg tggttaatca ggaactgttc gcccttcact
     1861 gccactgacc ggatgccgac gcgcagcggg tagatatcac actctgtctg gcttttggct
     1921 gtgacgcaca gttcatagag ataaccttca cccggttgcc agaggtgcgg attcaccact
     1981 tgcaaagtcc cgctggtgcc ttgtccagtt gcaaccacct gttgatccgc atcacgcagt
     2041 tcaacgctga tatcaccatt ggccaccacc tgccagtcaa cagacgcgtg gttacagtct
     2101 tgcgcgacat gcgtcaccac ggtgatatcg tccacccagg tgttcggcgt ggtgtagagc
     2161 attacgctgc gatggatccc ggcatagtta aagaaatcat ggaagtaaga ctgctttttc
     2221 ttgccgtttt cgtcggtaat caccattccc ggcgggatag tctgccagtt cagttcgttg
     2281 ttcacacaaa cggtgatacg tacacttttc ccggcaataa catacggcgt gacatcggct
     2341 tcaaatggcg tatagccgcc ctgatgctcc atcacttcct gattattgac ccacactttg
     2401 ccgtaatgag tgaccgcatc gaaacgcagc acgatacgct ggcctgccca acctttcggt
     2461 ataaagactt cgcgctgata ccagacgttg cccacataat tacgaatatc tgcatcggcg
     2521 aactgatcgt taaaactgcc tggcactgca attgcccggc tttcttgtaa cgcgctttcg
     2581 caccaacgct gatcaattcc acagttttcg cgatccagac tgaatgccca caggccgtcg
     2641 agttttttga tttcacgggt tggggtttct acaggacgta acataaggga ctcctcatta
     2701 agataaaaat actggtcaac ctttaatctg attagttaac caattctgtg ttgcacacag
     2761 ttatctgtga gaaacccatg gaagagaaat ggaaataaaa agaactgaca ccgcgatcaa
     2821 aaaaccaaaa actgcaatac ccacagcgtg atatggatcg cattaatctt taaaacagcg
     2881 ttgacagaag ccataaaata agcaatcgaa cgttcgttcg agagtgttgg gaacgataaa
     2941 ctctcatgac atatggctac ggtgaatatt ttggggggga attctccgaa cacggggagg
     3001 ataatttttg cacagagaaa ttacgatatg aaacgcgggc acagttatca ccagcccgcg
     3061 aaatgagagg atcaggatgc ggttaatata ccgccaatca tcgcgcgtaa tccctgagca
     3121 aacgacgctt cacgcgccag gtcttccgct tcgatatcgg ccagtacacc gtaggtcaga
     3181 tccagcagta aacgtgaagt cattgccgtg ttgatgtctg ggcttatctc gcctttttgc
     3241 tgggcatcac gcatccgctg ggcaacaaat tcagtgatcg tcatatgctt atttttaagc
     3301 atggcggcga cctgtgggtt acgcatccct tccgccataa tttcgaccac cagcgcccgt
     3361 tgcccaaagg cttcatgggt gagagagaca atagactcga ccatatagtc aacaaaatga
     3421 atcccttcaa tcggttcccg gaaacgagcc agcgccctct cctggtcctg taagataatc
     3481 gcctgaatca aggcttcttt ggagatgaaa tggtgataga gcgtcccagg actaatggcg
     3541 caagatttac agatggcttt catcgaggca ctgtgaaatc cattttctga aaaaatctct
     3601 ctggcagcat tgaggatccg ggtccgtgtc ggttgtgctt cagtctgcat gttatccatc
     3661 attaaaccat tatagttagc gtgaataact agtttaccgc gttgtcaggt gagcgcctat
     3721 cattgctgtt gaatagtgcg aaggtacact ctattcgtat acttttcacc cagcaattgg
     3781 ttaaccaata gccgtgattt ggctgccagt tgtttagcag agacgttgat gaattgcaca
     3841 cgctatcccg gtaaacaaca catacgaccg tttaccggtt cgttagtgta ttaattgagc
     3901 tcctgtaccc caccaccgga gacggtgaga atttgtccgc ttacccagct ggctgcaggc
     3961 gagcaaagga acagcgccgc gttagcaata tcttgcggtt ggcccagacg tctgatcggc
     4021 gtgtgctgta acattttttg ttcaa
//
LOCUS       DAZXUK010000300         4042 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZXUK010000300 DAZXUK010000000
VERSION     DAZXUK010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.300"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(126..284)
                     /locus_tag="ACH3L8_003767"
     CDS             complement(126..284)
                     /locus_tag="ACH3L8_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFT4086679.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEI
                     LRVLEVVA"
     gene            complement(886..1077)
                     /locus_tag="ACH3L8_003768"
     CDS             complement(886..1077)
                     /locus_tag="ACH3L8_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFT4086680.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENR"
     gene            1142..1465
                     /locus_tag="ACH3L8_003769"
     CDS             1142..1465
                     /locus_tag="ACH3L8_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFT4086681.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1674..2105
                     /locus_tag="ACH3L8_003770"
                     /pseudo
     CDS             1674..2105
                     /locus_tag="ACH3L8_003770"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="ACH3L8_003771"
     CDS             2143..2673
                     /locus_tag="ACH3L8_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFT4086682.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="ACH3L8_003772"
     CDS             2680..2913
                     /locus_tag="ACH3L8_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFT4086683.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="ACH3L8_003773"
     CDS             complement(3459..3851)
                     /locus_tag="ACH3L8_003773"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086684.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          965 a          918 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcagc ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAZXUK010000301         4019 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZXUK010000301 DAZXUK010000000
VERSION     DAZXUK010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.301"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..2955
                     /gene="sigA"
                     /locus_tag="ACH3L8_003774"
     CDS             <1..2955
                     /gene="sigA"
                     /locus_tag="ACH3L8_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFT4086685.1"
                     /translation="VNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVSGGGVLTLKQDT
                     DLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGDNLHKIGSGTLD
                     VKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSESGNGEIYFTRNG
                     GTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGNVSGNISINHII
                     NTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGNNNCPIPFLCQK
                     DYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNLENATLSIGRDA
                     NVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSAGESSFNGSLSA
                     QNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIELAGTLTLTGTP
                     TENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILFGVDQYKEDNLD
                     KTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQSLKTRDSLLLFS
                     NIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGSNNSLSVDIQKV
                     GNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEHTTWTLTGYKVA
                     ENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARIMSGAGSASGGY
                     SDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSVGAGLYASAIFD
                     SGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHVTEDSWIEPQAE
                     LVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQ
                     FDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLEFEKSAFGKYNV
                     DNAINANFRYSF"
     gene            3014..3583
                     /locus_tag="ACH3L8_003775"
     CDS             3014..3583
                     /locus_tag="ACH3L8_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086686.1"
                     /translation="MTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNAHILE
                     HGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTDTITE
                     ATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLFQGKM
                     STDGNNYYIIKGKVSSVLLLLKMLQINHA"
BASE COUNT         1253 a          844 c          921 g         1001 t
ORIGIN      
        1 gtaaacaatt taaaaaagaa tttcacaaac actatgcagc tggatggtaa aaaacaagag
       61 ttatcatcga ttataaaaga taaggacctg tctgtctcag gaggaggggt attaacgctc
      121 aagcaggata ccgatcttgg cattggcggg cttatattcg ataagaacca gacatataaa
      181 gtgtacggaa aagataagtc ttataaaggt gccgggatag atattgataa taataccacc
      241 gttgaatgga atgttaaggg cgttgccgga gataatctgc ataaaatagg tagtggtact
      301 ctggatgtaa aaatagcaca gggaaataac cttaaaatag gtaatgggac tgtcatcctt
      361 agtgctgaaa aagccttcaa taaaatttac atggccggag gtaaaggtac ggtaaaaata
      421 aatgccaaag acgctttaag cgaaagcggt aatggcgaaa tctattttac cagaaatggc
      481 ggaacactgg atctaaacgg ctatgaccag tcatttcaga aaatcgcagc aacagatgcg
      541 ggaacaaccg taacgaactc aaacgtgaag caatcaacat tatcacttac taatactgat
      601 gcatatatgt accatgggaa tgtatcaggt aatataagca taaatcatat tatcaatact
      661 acccagcaac ataacaataa tgccaatctg atctttgatg gctcagtcga tatcaaaaac
      721 gatatctctg tccggaatgc acagttaaca ttacaaggac atgcgacaga acatgccata
      781 tttaaagaag gcaataacaa ctgtccaatt ccttttttat gtcaaaaaga ctattctgct
      841 gccataaagg accaggaaag cactgtaaat aaacgttaca atacggaata taagtccaac
      901 aatcagatag cctctttttc ccagcccgac tgggaaagtc gtaaatttaa tttccggaaa
      961 ttaaatttag aaaacgcaac cctgagtata ggccgggatg ctaatgtaaa aggacacata
     1021 gaggctaaaa actctcaaat tgttctggga aataaaactg catacattga catgttctca
     1081 ggaagaaaca ttactggcga aggttttgga ttcagacaac agcttcgctc cggggattca
     1141 gcaggcgaaa gtagtttcaa cggcagtctg agtgctcaaa acagcaaaat aactgttggt
     1201 gataaatcaa ctgttactat gactggtgca ttatccttaa ttaatacaga cctgattatc
     1261 aacaaaggag ctactgttac cgcccaggga aaaatgtatg tagataaagc tattgaactg
     1321 gccggaaccc tgacattaac aggcacccct acagaaaata ataaatacag cccggcaatc
     1381 tatatgtcag atggatataa tatgacagaa gatggtgcca cgttaaaggc tcaaaattat
     1441 gcctgggtca atggtaatat aaaatcagac aaaaaagcat ctattctgtt tggtgttgac
     1501 cagtataaag aagataacct ggacaaaacc acacacacac cgctggctac aggtttgctg
     1561 ggtggctttg atacttctta taccggaggt attgatgctc ctgcagcctc agccagcatg
     1621 tataacacct tatggagagt aaacggacag tcagccctgc aatcattaaa aacccgcgac
     1681 agtcttttgt tgtttagtaa catagagaat tcgggtttcc atactgtgac agtaaacaca
     1741 ctggatgcca ctaatactgc tgtgattatg cgggctgatc tgagccagtc tgtaaatcaa
     1801 tcggataaac tcattgttaa aaatcagtta accggaagca ataacagtct gtcggtcgat
     1861 atacagaaag tgggaaataa taactcagga ttaaacgttg acctgataac agccccaaaa
     1921 ggaagcaata aagagatatt taaagccagt actcaggcca taggtttcag caacatatct
     1981 cctgtgatca gcacgaaaga ggatcaggaa cataccacgt ggaccctgac cggatataag
     2041 gtggctgaaa atacagcatc ttccggtgca gcaaaatcgt atatgtccgg taattacaaa
     2101 gccttcctga cagaagtcaa caacctgaat aaacgaatgg gggatctgcg tgacaccaat
     2161 ggcgaggccg gtgcatgggc ccgcatcatg agcggagcag gttcagcttc tggtggatac
     2221 agtgacaact acacccatgt gcagattggt gtggataaaa aacatgagct ggatggactt
     2281 gaccttttca ctggtctgac tatgacgtat accgacagtc atgccagcag taatgcattc
     2341 agtggcaaga cgaagtccgt cggggcaggt ctgtatgctt ccgctatatt tgactctggt
     2401 gcctatatcg acctgattag taagtatgtt caccatgata atgagtactc ggcgaccttt
     2461 gctggactcg gaacaaaaga ctacagttct cattccttgt atgtgggtgc tgaagcaggc
     2521 taccgctatc atgtaacaga agactcctgg attgagccgc aggcagaact ggtttatggg
     2581 gccgtatcag gtaaacggtt cgactggcag gatcgcggaa tgagcgtgac catgaaggat
     2641 aaggacttta atccgctgat tgggcgtacc ggtgttgatg tgggtaaatc cttctccggt
     2701 aaggactgga aagtcacagc ccgcgccggc cttggctacc agtttgacct gtttgccaac
     2761 ggtgaaaccg tactgcgtga tgcgtccggt gagaaacgta tcaaaggtga aaaagacggt
     2821 cgtattctca tgaatgttgg tctcaacgcc gaaattcgcg ataatcttcg cttcggtctt
     2881 gagtttgaga aatcggcatt tggtaaatac aacgtggata acgcgatcaa cgccaacttc
     2941 cgttactctt tctgataaca gcccgggccg cgtttgcggc ccttcttcta ccggagagaa
     3001 tatgtattac cctgtgacag actatatcgc tcttgctctc attattagct ttctttttct
     3061 gacattattt atctgcctgt tatgccttaa acatgagcga ataaaaaaag aaactatcag
     3121 gcaaaaaaat gcacatattc tggagcatgg ctggaatgca actgagttct catggttccg
     3181 atacggacag tataacgaaa cgggtattta catctcgatc gagaagacca ttatcattac
     3241 aatcaccgtt tctggtgggt gctttaaaaa agaatacagc attgtctccc acatgctggt
     3301 cactgacacc ataacagagg ccaccctgta cgaaaacggg ctgtatacgc gccacatcag
     3361 actcagtcgc ccagtatctg acagcaaata ccccttgcca cccggatctc agctcattaa
     3421 aaatatgacc ttacgactgc gcctgcagga tcagcaggaa gaaacatccg tgacattatt
     3481 tcagggaaaa atgagtaccg acggaaataa ctattacatc atcaaaggta aagtaagctc
     3541 agtacttctg ttattaaaaa tgctgcagat taatcacgcc tgatctctgt cccggtattt
     3601 tagcccgagg tatattctga aatatcattc atcatgaccg gcgtttgtat aaacgccggt
     3661 cattcgctaa cagcttcacc gttccttccc ccatccaggt tccgcatgag cgtgttgtaa
     3721 tgttcataag cgtattatcg taagcttccg gggaactcat attgacgcgt ttttcaggcg
     3781 cgcgtcatcg gcggcgcata cgaacgatcg tggtttgata acggttgatt tattttgttg
     3841 cacaggggcg atcgccaggc aggtaccgga ggcgggctct gaagtactga gtagggtgat
     3901 cgcgtcaggc cgcgaagtac tgactcgggg attaatagca ggtgatgagt aagcgtacag
     3961 cgagggccgt attgacgggg atgtgttatt cagctggcag tgctatgcgc cacggaagc
//
LOCUS       DAZXUK010000302         4001 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZXUK010000302 DAZXUK010000000
VERSION     DAZXUK010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.302"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..203
                     /locus_tag="ACH3L8_003776"
     CDS             <1..203
                     /locus_tag="ACH3L8_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086687.1"
                     /translation="KGWATSLRQKQKKLIMLPSETMIWQPEFTDKTLSRKPGAVQRAF
                     PRVVKERPWKYPTAPKKSQSVA"
     gene            complement(217..408)
                     /locus_tag="ACH3L8_003777"
                     /pseudo
     CDS             complement(217..408)
                     /locus_tag="ACH3L8_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            406..730
                     /locus_tag="ACH3L8_003778"
                     /pseudo
     CDS             406..730
                     /locus_tag="ACH3L8_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(760..3672)
                     /locus_tag="ACH3L8_003779"
     CDS             complement(760..3672)
                     /locus_tag="ACH3L8_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFT4086688.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKVFYERSEKSVGYWVGGIRRAAQKA"
     gene            complement(3729..>4001)
                     /locus_tag="ACH3L8_003780"
     CDS             complement(3729..>4001)
                     /locus_tag="ACH3L8_003780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086689.1"
                     /translation="YIIISFKNDSLRTLRWHTEFGYLNRGDMFTSEQHRYSNEKKEFQ
                     RRVYLRGDAFINLYTNFYSCIMTGLQLLRHFQRIALPAYHPTFFIP"
BASE COUNT         1015 a         1062 c          990 g          934 t
ORIGIN      
        1 ggaaaggctg ggccacatcc ctccggcaga agcagaaaaa gcttattatg cttccatcgg
       61 aaacgatgat ctggcagcct gagttcacag ataaaacact ctccaggaaa cccggggcgg
      121 ttcaaagggc cttcccgaga gtggtaaagg agaggccctg gaaatacccc acagccccga
      181 aaaagagcca gtcagttgct taactgactg gcattaaggc cgccggggac gtttttttac
      241 caccgggagt ttgggcataa cggtgctttc agtaagtacg acaggtactg tggaggcact
      301 tccgtaaaga agggcatcaa gctcctgctc cgacatacct gcaggcaaag gccatgaaag
      361 ccctgatttc cggaagcgag cgaacagtcc acaaactgtt gaacgtggtg tcagcggcgg
      421 acagggataa gccctcgtca gcagttccgt cagcactgcg acagcgttct ttacccggtc
      481 aaaacagcgt tgcggtgcac cacgcctgac ggatgaactg cgtgctcagg gttaccactt
      541 taacgtaaaa accgtggcgg caagcctgcg ccgtcagggg ctgagggcaa aggcctcccg
      601 gaagttcagc ccggtcagtt accgcgcaca tggcctgccc gtgtcagaaa atctgctgga
      661 gcaggatttt tacgccagtg gtccgaacca gaagtgggcg ggagacatca cgtacttacg
      721 tacagatgaa gttcggttac atcctgtcag cacggaacct cacgcttttt gagcggcacg
      781 acggatcccc cccacccagt atccgacaga cttttcactg cgttcgtaaa aaaccttctt
      841 ccctcccagc atatccacta attccggtga catatcctgc agataagtct gcaaatcatg
      901 ccccagatct tccggtgact gcaactcata aaccgcgcgc cacacatcac ccttccttat
      961 tttaagcccc gggttaagaa tatactgtcc gtggttcagc ctcagaaaaa gcttttttcc
     1021 ataaggattt ttgctgttaa cttcatgttg agagagctgg ctgctgatat aactccttcg
     1081 tttgcggcgt tccggcagca acgcttcagg aagatgaacc acacactctg ccagtcgtgc
     1141 cgcactgaag gcttccgaaa aaaacagagc acttcccaga tgcgtgtgga acagcgcctg
     1201 catcaggatc accagaaaca ttgccatctg ccggttgtcc agtttgatga ggcgtccttc
     1261 cacctgcagt gacagacttt caggtttcag cagggtatac aactgcgcac ttttctgttg
     1321 cgcataacgg ggcgtactga ccgccgctga cagaacttga tgataagccg ccagcccgtc
     1381 attaccggtc agcaagggat ctgcaccacg ctccagcagt aactgaacca gtgcaatatt
     1441 acctgcccgg gctgctgaca tcagcggagt aagattaaac ggcgaacggt gatcaacacc
     1501 ataacgttca atatcccgca acacggcaac cgggttattc attgagtatg gccggaaata
     1561 cttcagctcc agtaactgca gagtcttcgt tctgggctgc cttgtgggct taaaatcttc
     1621 ccggtacagt gcattataca gcgggaacag ggaataaatc agactgtact ccagcgcctg
     1681 cagttgtatg gttttatcgg ctgtacctgc gaggatatgc tgtttccact gtcgtgcatc
     1741 ctctgcggtg ataatctgcc atggcatctc agatgtctgc aggatccggc tacggatggt
     1801 gtccgcctgc tcctgcttcc cctgtttttc aagcagatgt gcggttttct gccactcatc
     1861 acggctggaa atctcttcct gcagattcag aggctcttcc tgatgcgtga gcgcaagcag
     1921 actccacaga ggatgctcca cctgctgttc aacaagatac acattgtgtt tagcacgggt
     1981 gagcgcaaca tacagcgcgt tggtaaaaaa tttatagatt tccgctgaac gatcctgcct
     2041 gtcccgcgga cgggaataac gggcctcgcc ctcaagatca gccggtgtaa gaccctcgca
     2101 aatatcatca aatgcctgcc gggcagcgct tactatattg taaaggatca ccgtttcgta
     2161 ttccagcccc ttaacctcct gaaccgaaaa gaccagcggc gtactgaacc agcatcgcgc
     2221 ctgtgctttc tgttccggat gcatcacaat gaccgctaca cgattcgaaa gtgaggtttt
     2281 ggcattcagt tcctgctttg tctcttccct atcgtccagc aaccggatgg tcccttctgc
     2341 ctgaccgcag cttctgacaa aatggtgact ttcccgatca atagcactaa agcggacctg
     2401 tttgagtctc agcaaccggt tagccagtgc agtgacgtgg tgcccgttac ggtaattcgc
     2461 ctgcaggata ttaacggttg tgtcgtttcc ctgttgctgc cggaaaaaga gacttttgag
     2521 cgaactccat gagaaaaagc tcggatgaac tatctgattg gcatcaccac agagtaaaaa
     2581 ctgaccggga tgacgtagcg ttttcagtac cagttgcagt tgaacaggcg tcatatcctg
     2641 aacttcatca acaaaaatga catcataacg gggagttgcc aacgaaagat actgatgact
     2701 gagcagatta ctgtcatact gatgcgactg tttcagccat gcgatataac gttcaaacag
     2761 gacatagacc gtcgggcggt cctccatgcc atagagcgac tgccgtatcc ccagtgacag
     2821 atacgcctcc cgggacagcg gcccattccc ggaggcaacc gcaccgatca cgcccctgaa
     2881 ctcctcatac agggtatggg cagcccccag tgcggcgata gccctgttgc ggggcagcca
     2941 gtcgctgaac gcactgaaag tcacctccct gccttccgga atgcgcagag tttccagaaa
     3001 ttccgtcagc gaaagaaagt caatattctg tacctcactc ccttcccctg aagcgtcata
     3061 gagagtacgc gctttttcca ccagaaatga ggaaaggctg agatacagta tatcaccagc
     3121 agcctgcttc attttttcca gtaccagcgc cgtcttacca cttcccgccg agccaacaat
     3181 caccagcggt agcgggtgtt cgtacagtgc ctgctgatca tcatcaaagg acagcatttt
     3241 atccaggcgg tggaaacgcc catgagacgg attgatatac gtcagcgctt cagtcgcgat
     3301 atcaacagga tccggcactg gttgttgttg taagcgccct tcatcaatca ccacgtttct
     3361 gcgaagaaaa cgtgaagtgt tataggcatg attgcgaata tattccagaa caaggcagat
     3421 agtttcaccg tggtagcgat acagcgaaaa cagtaatcgg tcccggatac tgagccgggc
     3481 gcggtaaaga ttggtgtcaa ttttacgaac atcagcctga acaaagttat cggtggcaat
     3541 agcctgtgca aattttttat aacccgggat cgtgtcagga ttcagagtat gataaagaca
     3601 gattttcatg gctgccggtt tccgtcgtgc aaaaaacatg ctgattttac gaaggaatgc
     3661 cttactgaac aaccttgccc ggaatacccc ccgaaatcag atggaacaat cagaaaaatg
     3721 ctctgcagtt atggaataaa gaaggtgggg tggtacgccg ggagggcgat ccgctggaaa
     3781 tggcgcagta gctgcaggcc tgtcatgatg caggaataga agttcgtgta caggttaatg
     3841 aaagcgtcac cccgtaaata aactctgcgc tgaaattcct ttttttcatt ggagtacctg
     3901 tgttgttctg aggtgaacat atcacctctg ttcaggtagc caaattcagt gtgccacctc
     3961 aaggttctta gtgagtcgtt tttaaaagat atgataatat a
//
LOCUS       DAZXUK010000303         3977 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZXUK010000303 DAZXUK010000000
VERSION     DAZXUK010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.303"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..287
                     /locus_tag="ACH3L8_003781"
     CDS             <1..287
                     /locus_tag="ACH3L8_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFT4086690.1"
                     /translation="EWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVK
                     LSMPELEELAAAMVQAQVDRNDEIYRRQRELKEELNSLKDLNLVRNFIVE"
     gene            complement(488..1345)
                     /gene="sitD"
                     /locus_tag="ACH3L8_003782"
     CDS             complement(488..1345)
                     /gene="sitD"
                     /locus_tag="ACH3L8_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFT4086691.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1342..2199)
                     /gene="sitC"
                     /locus_tag="ACH3L8_003783"
     CDS             complement(1342..2199)
                     /gene="sitC"
                     /locus_tag="ACH3L8_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFT4086692.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2196..3023)
                     /gene="sitB"
                     /locus_tag="ACH3L8_003784"
     CDS             complement(2196..3023)
                     /gene="sitB"
                     /locus_tag="ACH3L8_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFT4086693.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3023..3937)
                     /gene="sitA"
                     /locus_tag="ACH3L8_003785"
     CDS             complement(3023..3937)
                     /gene="sitA"
                     /locus_tag="ACH3L8_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFT4086694.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1054 a         1006 c          985 g          932 t
ORIGIN      
        1 ttgaatggaa cggtcgtatc tggaatgctg gtcccgactc actgggccgc ctgtccccgg
       61 tagtcatgct ggcaaaatct gtcacagcac aaacacatat ggcgtggagc gatgccgata
      121 atcagcaggt gaaactgtcg atgccggaac tggaagaact ggcggcagca atggtgcagg
      181 cgcaggtcga tcgcaacgat gagatttatc gccgtcagcg ggagctgaag gaagagctga
      241 atagtctgaa ggatttgaat ttggttagga attttatcgt ggaataacag aagctgcggc
      301 acgtcgtatg caggaacgtg ccgcggttgg ctggtaaact ttcgatagtg cgagtattga
      361 atgatttcta ggcgttatcg attttacgta ttttttgcat gatagaattc gtacctcctc
      421 ccaacgacct tccatggctt tccggacttc tgtaggctgg cggacgatgc tgtcctcgga
      481 tgttgtgcta attgggtgat aacagatcct gtgtatgggc gtttcccttt gtgcgtgcgt
      541 tgatgctggt aacggtaaac gtcatgatga acacgaccgc gaataagacg acaatcgtcg
      601 gagctggtgc gctgtcgata aaaaaggaca gccagacacc agtcattgac actattaccg
      661 atatgccagt cgccagtagg agcgcaatat gaaagcgttg tgtcaggagt actgcaatgg
      721 cgccaggcgc aatgagcaga gaaatagaga gaatgatacc tactgctttc agcgtcgcca
      781 caatggtcag ggaaaccatg cacagcagcc cataatgcag ccatctcgta tgcaaaccgc
      841 ttacctgcgc ctgttgataa tcaaagctga acagcaaaaa atcccgccat tttacgctaa
      901 taacaagtgt aaccagcccg gcaataatca ccgtctggat tatgtcgccg atggttatcc
      961 cgagcatgtc gccgaaaaga atatggtcaa gatgtacgtc tggtttaatt gcaatataca
     1021 agatcagacc tgcggcaaac atgccagaaa aaacaatccc cataactgta tcttgcttga
     1081 ttcggctgtt gtctttcagg tatcccgtcg ccaccgcgca gaatactccg gcgacgaatg
     1141 cccctgtagc cagaggtaac cccagaatcc aggtaagcac gatgccagga aaaaccgcat
     1201 gactcatcgc gtcacccatc agtgcccaac ctttcaggac cagaaagacc gacagcagcg
     1261 cacagggtat ggaaactacc agtgaaatca gcagtgcgtg actcataaag gtgaactgca
     1321 gtggctccag taataatgcc atcatgagcg atcctccagc gctttatgcg cacgccgacg
     1381 atttgccaaa agtccatggg tgggcgcaaa tacaaatgct aaaagaaaca gcagtgtctg
     1441 cgccaccacg atgattccac ctgtcgcgcc gtccagaaag taactgaccc acgcgccgag
     1501 gaaactggtt acactgccaa tagttacagc aattatgagc aggcggggaa aacgatcggt
     1561 taacaaccat gctgttgcac cgggagtcac caccagacag atcaccagaa aggcaccaac
     1621 cgtttgcagc gccgcaacgg tcgaaaccga cagcagggtg aagaagagga tttttaatct
     1681 gcccggatgt aatccaatag cacgggcgtg attctcgtcg aaaaaggtca ccataagatc
     1741 tttccatttg aaaaaaagca cgatgatcga gagaatgcca atgatagtga gttgcaggat
     1801 atccgccgga tcgattgcca gaatattgcc caggacgatg gtctgaatgt tcacggatgt
     1861 cgggttcaat gacaccataa acagccccag gccaaaaaag gacgagaaga tcaggccgat
     1921 gatggcatct tcttttaagc gggtgcgttg gttaagaaac agcatgctgc ccgctgccag
     1981 tccgccggaa aagaaggctc ccagagaaaa cggaagcccc agcatatatg cccccgcaac
     2041 acccgggaca atagagtgcg acagcgcatc gccaatcagc gaccagcctt ttagcatcag
     2101 atagcatgac aggaaagcgc agagaccgcc gaccattgcc gagacccaca tcgcattgag
     2161 catatattca tagctgaagg gttccagtag cacgttcatc ttgcttccct ctgcaccgct
     2221 gacggtcgat gtgccacaaa agggcgttca tcgtcagtaa taatgctttc ttccgagccg
     2281 ttaagtgtga catggcgcag tacgccgcta aaagccagct ccaggttctc ggctgtaaaa
     2341 gtggtgtctg tcggcccgct tgccaggacg gtgcctttga ccatgaccgt ataatcgcaa
     2401 aatgtcgtga ctgagccgag attgtgcgtt gagacaagca tcgttttgcc ttccgcgcgt
     2461 aactccctca acaggctgat gattttcgct tcggttttca catcaacgcc ggtaaaaggt
     2521 tcatccagca ggatcacatc accctgctgt gcaattgctc tcgccagaaa gacgcgtttc
     2581 ttttgtccac cggaaagctc gccgatttga cgatggcgaa aatccaccat atcgacacgt
     2641 tccagcgcat cggtgacaat ctgacgatcc ctctttttgg ctatacgcaa catacccata
     2701 tgcccatagc ggcccatcat caccacatct tccaccagta caggaaatga ccagtcaacc
     2761 tcttctgact gagggacgta ggcaaccagg tttttctgta gcgcctgtcg cgtgggaata
     2821 cccagaacag atatctttcc gctggtcaga cgcacaaatc ccataatcgc tttaaacaac
     2881 gtcgattttc cggaaccgtt taccccaacc agagcggcaa tagatccact tggtactgtg
     2941 aaggatgcat cgcgcagtgc tgtgtgcccg ttacgccagg tgacagtaac atcattaacg
     3001 acaatgcctg cagattgcat cattatttct cccttttccc ggctttaatt ccctgtacca
     3061 gcgtactggt agtaacctta agaaggtcga tatacgtagg taccgggccg ttttctgtgc
     3121 tcagggaatc gacatagagc acaccgccgt agtgtgcgcc ggtttcacgc gcaacctgac
     3181 gcgctggttt atcggaaatc gtactctcgc taaagactgc cgggatatga tttttcttaa
     3241 ctatatcaac aaccttacgt acctgctgcg gtgttccttg ttgatcggca ttaatcggcc
     3301 acagataaag ctcttttagc cccaaatcgc gtgcgagata agaaaaagcc ccttcactgg
     3361 tgaccatcca tcgctgattc tcagggagtt ccgtaatctg cttacgcagg ggggcaaggg
     3421 tttgggtaat cttggcttta taagtatcgg cattacgttg gtaggtttgt gcatttgccg
     3481 gatcgtattt tatcaacgca tcacgaatat tatcgacgta aatcagagca ttatctggcg
     3541 acatccatgc atggggatta ggtttgccct catagggtcc ttcggtgatc cctactggcg
     3601 tcacacccga agagacaatt acttctggaa ccccattgag atgctggtaa aagcgttgga
     3661 accacaattc cagattcata ccattggcga gaatcagttg tgccccctgc gcacgtttaa
     3721 tatcgccagg ggtaggctga tactcatgaa tttctgcacc aggcttggtt atggatgaga
     3781 cttctgcagc atctccagcc acgtttttgg ccatatccgc gatgatggta aatgttgtaa
     3841 tgaccttgaa tttttcagtt gcacttgcct gaaatgcgat cgagcaggtg agggcgaggc
     3901 accccaagag catggttact ttttttattg agtgcataat ttagtaccta tagtgagcaa
     3961 aattagaaca aacatag
//
LOCUS       DAZXUK010000304         3926 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZXUK010000304 DAZXUK010000000
VERSION     DAZXUK010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.304"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(77..2053)
                     /gene="speA"
                     /locus_tag="ACH3L8_003786"
     CDS             complement(77..2053)
                     /gene="speA"
                     /locus_tag="ACH3L8_003786"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFT4086695.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2062..2193)
                     /gene="yqgB"
                     /locus_tag="ACH3L8_003787"
     CDS             complement(2062..2193)
                     /gene="yqgB"
                     /locus_tag="ACH3L8_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFT4086696.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATAVNCFTLNKII"
     gene            complement(2287..2430)
                     /gene="yqgG"
                     /locus_tag="ACH3L8_003788"
     CDS             complement(2287..2430)
                     /gene="yqgG"
                     /locus_tag="ACH3L8_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFT4086697.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2541..2792)
                     /gene="yqgD"
                     /locus_tag="ACH3L8_003789"
     CDS             complement(2541..2792)
                     /gene="yqgD"
                     /locus_tag="ACH3L8_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFT4086698.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2848..>3926
                     /gene="metK"
                     /locus_tag="ACH3L8_003790"
     CDS             2848..>3926
                     /gene="metK"
                     /locus_tag="ACH3L8_003790"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFT4086699.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYG"
BASE COUNT          997 a         1052 c          966 g          911 t
ORIGIN      
        1 ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa tcgacccgcc
       61 agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa cctgcctcga
      121 actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc acttgatcgc
      181 ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg tccgccacgg
      241 tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg aacacgtcaa
      301 ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga tatgcaccga
      361 ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt ggcattgtcg
      421 tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca caggtaatat
      481 ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt agaaccggga
      541 acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa ttgacgtaca
      601 ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca cggttttgcg
      661 gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct gcccatgcac
      721 gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc agatccatct
      781 ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc atttcctgcc
      841 aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg gtcggcaccg
      901 tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg tgcgcagtca
      961 ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct tcacacgcat
     1021 cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt acggagcagt
     1081 cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg tcgaagcact
     1141 gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca cgaacgcctg
     1201 tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt agttgcaggc
     1261 tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga gtcgcagcca
     1321 ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc tgcgaagcca
     1381 gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca tccagcacaa
     1441 tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc ttctcgccaa
     1501 ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg acgctacgag
     1561 tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct tccagaccca
     1621 gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta actttgatcg
     1681 gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt ttgaacgcgg
     1741 cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt gcaggcagac
     1801 gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga gcttccggga
     1861 cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag tagttattgc
     1921 cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag ctcattgcaa
     1981 cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa cccatagaca
     2041 tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt taacggctgt
     2101 cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca gtgtatgctg
     2161 acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat aaccgaacgt
     2221 aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag cgcccttgtt
     2281 cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat gggtataaca
     2341 tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac cagaacggtg
     2401 agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg gccgcctgtt
     2461 aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc aacacgaaag
     2521 aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac aatggcagaa
     2581 aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc cagccacgtt
     2641 tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa ttgacctaaa
     2701 atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc agtgttttta
     2761 acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag tttgagctaa
     2821 ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc cgagtccgtc
     2881 tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt agacgcgatc
     2941 ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac cggcatggtt
     3001 ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat cacccgtaac
     3061 accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa ctcctgtgcg
     3121 gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga ccgtgccgat
     3181 ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac taatgaaacc
     3241 gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg tcaggctgaa
     3301 gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca ggtgactttc
     3361 cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac tcagcactct
     3421 gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat caagccaatt
     3481 ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac cggtcgtttc
     3541 gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat cgttgatacc
     3601 tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc atcaaaagtg
     3661 gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc tgctggcctg
     3721 gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc gacctccatc
     3781 atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct gctggtacgt
     3841 gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct gcacccgatc
     3901 tacaaagaaa ccgcagcata cggtca
//
LOCUS       DAZXUK010000305         3864 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZXUK010000305 DAZXUK010000000
VERSION     DAZXUK010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.305"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACH3L8_003791"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACH3L8_003791"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFT4086700.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACH3L8_003792"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACH3L8_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFT4086701.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3862)
                     /gene="bglG"
                     /locus_tag="ACH3L8_003793"
     CDS             complement(3053..3862)
                     /gene="bglG"
                     /locus_tag="ACH3L8_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFT4086702.1"
                     /translation="MTKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSSGIEK
                     EYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHCQFAI
                     KRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQMSGN
                     MEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASINDSDE
                     SLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
BASE COUNT         1017 a         1027 c          854 g          966 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttctt
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg attt
//
LOCUS       DAZXUK010000306         3858 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZXUK010000306 DAZXUK010000000
VERSION     DAZXUK010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.306"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACH3L8_003794"
     CDS             complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACH3L8_003794"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFT4086703.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(1572..1768)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2005..3654
                     /gene="pgi"
                     /locus_tag="ACH3L8_003795"
     CDS             2005..3654
                     /gene="pgi"
                     /locus_tag="ACH3L8_003795"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFT4086704.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT          974 a         1030 c          974 g          880 t
ORIGIN      
        1 ttttctgctc attattagct ccttctgatg ccattctatt tcaggaagga gtgtccgtta
       61 aactcaggct acctcatagt gacaagaaaa tcaatacggc ccgaaatata gcttccaggc
      121 catacagtat ttactcaaac aaattactat gcagtttttg caccacctgc tcggcatctt
      181 cgccgggcac caggaagcac aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt
      241 tgaacggttc cagtacgccg aatacctctt tgccaacgcc gcaggctttt gacaggtcat
      301 tgccaatcaa cgcgaccagc gccagacctt cttccacctc cacccggcac agtgcggaaa
      361 gctccatcag cagagattgc gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat
      421 caagggttaa tgccacgctc acttctgacg tggtgattaa gtctaccgaa atattatgcc
      481 gcgcgaggat gccgaaaact tccgcgagga agccgcgaga atgcagcata ttcaggctgt
      541 gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt
      601 cagttttatt gcacaccagc gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga
      661 ccgggatatc gctgcgtact gcgggtagca acgttgccgg atgcagtact tttgcaccaa
      721 aagttgccat ctctgccgct tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa
      781 ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt ccagatatca acacgagatg
      841 cgtgtaaagc ctccgccagc aaggctgccg tataatcgct gcctccacgg ccaagcgtcg
      901 ttgtacgacc tttattttcg ctaccgataa atccctgggt gatcactaag ccttcattga
      961 ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc ggctacatct ggctctgcac
     1021 gaccaaatcg gtcgttggta cgcatcactt tacgtacatc aaaccactgt gcctgaacat
     1081 cgcgttcgcg caggatttcg acaaacagca gggtcgacat cagctcgccg tggctgacca
     1141 gctcatctgt cagcgccgga gacgttgcca gcgccgccgc ttctgccaga acagtaatgt
     1201 tctccagcag acgttcaatc tcttcacgga taacgttcgg gtaacgcaga cgttccagaa
     1261 tggcaaactg gatgttgcga atagcgtcga gtttttcgaa tcgctcgcca ggttccagcc
     1321 cttccgctaa agcgaccagc agattagtga taccagcaga agccgagagg actactaaac
     1381 gcacgttggc atcagaaagc acaatatcag cgctgcggtt catggcgtca aaatcagcta
     1441 cgctggtacc gccaaatttg gagacaacaa tttcagacat aactacctcg tgtcagggga
     1501 tccattttca gccttggcac aagggaagag cggaagacgg gtgggcgcag agcgatactt
     1561 cgctactatt ttcacccaga agtgctccac cacttgcgaa acgcccgact gcgaacgctt
     1621 ctggtgacaa cccaggggat tcagcccctg tagccgatga tgaacgtggc cagccgttca
     1681 atcacctcgg cgatgcaccc cctcaggtgt tatcacagga ctggctcctc caacaccgtt
     1741 acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg tagtacattt ataaataaag
     1801 ggtgagcggg gcggttgtca acgacggggt catgcggatt tttcatccac tcctggcggt
     1861 cagtagttca gctaataaat gcttcactgc gctaagggtt tacactcaac attacactaa
     1921 cggcactaaa accatcacat ttttctgtga ctggcgctac aatcttccaa agtcacaatt
     1981 ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc caacgcagac cgctgcctgg
     2041 caggcactac agaaacactt cgatgaaatg aaagacgtta cgatcgccga tctttttgct
     2101 aaagacggcg atcgtttttc taagttctcc gcaaccttcg acgatcagat gctggtggat
     2161 tactccaaaa accgcatcac tgaagagacg ctggcgaaat tacaggatct ggcgaaagag
     2221 tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg agaagatcaa ccgcactgaa
     2281 aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca ataccccgat tctggttgat
     2341 ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga agatgaaaac cttctcggaa
     2401 gcgattatct ccggtgagtg gaagggttat accggcaaag caatcactga cgtagtgaac
     2461 atcgggatcg gcggttctga cctcggcccg tacatggtga ccgaagcact gcgtccgtac
     2521 aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg ggactcacat ctcggaagtg
     2581 ctgaaaaaag taaacccgga aaccacgctg ttcctggtag catctaaaac cttcaccact
     2641 caggaaacca tgaccaacgc ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat
     2701 gaaaaacacg ttgcgaaaca ttttgcggcg ctctcaacca atgccaaagc cgttggcgag
     2761 tttggtattg atactgccaa catgttcgag ttctgggact gggtcggcgg ccgttactct
     2821 ttgtggtcag cgattggcct gtcgattgtt ctctccatcg gctttgataa cttcgttgaa
     2881 ctgctttctg gcgcacacgc aatggacaag catttctcca ccacgcctgc cgagaaaaac
     2941 ctgcctgtcc tgctggcgct gattggcatc tggtacaaca atttctttgg tgcggaaact
     3001 gaagcgattc tgccgtatga ccagtatatg caccgtttcg cggcgtactt ccagcagggc
     3061 aatatggagt ccaacggtaa gtatgttgac cgtaacggta acgttgtgga ttaccagact
     3121 ggcccgatta tctggggtga accaggcact aacggtcagc acgcgttcta ccagctgatc
     3181 caccagggaa ccaaaatggt accgtgcgat ttcatcgccc cggctatcac ccataacccg
     3241 ctctctgatc accaccagaa actgctgtct aacttcttcg cccagaccga agcgctggcg
     3301 tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg atcagggtaa agatccggca
     3361 acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta accgcccgac caactccatc
     3421 ctgctgcgcg aaatcactcc gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa
     3481 atctttactc agggcgtgat cctgaacatc ttcaccttcg accagtgggg cgtggagctg
     3541 ggtaaacaac tggcaaaccg tattctgcca gagctgaaag atgataaaga aatcagcagc
     3601 cacgatagct cgaccaatgg tctgattaac cgctataaag cgtggcgcgg ttaatcatca
     3661 tgcgtgcctg atgccgatat gtaggccgga taaggcgttt acgccgcatc cggcaaccgg
     3721 agcctgatgc caatatcgac attaatgcat agtatgagga gttaaataac cttaaaaaca
     3781 atgccgacaa taagtcggca ttgttttatc agataaatcc ccttgtctgt gatttaacgg
     3841 aaatcatacc gtgaggtt
//
LOCUS       DAZXUK010000307         3806 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZXUK010000307 DAZXUK010000000
VERSION     DAZXUK010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.307"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            437..2089
                     /gene="ushA"
                     /locus_tag="ACH3L8_003796"
     CDS             437..2089
                     /gene="ushA"
                     /locus_tag="ACH3L8_003796"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
                     /protein_id="HFT4086705.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="ACH3L8_003797"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="ACH3L8_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="ACH3L8_003798"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="ACH3L8_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3595)
                     /locus_tag="ACH3L8_003799"
                     /pseudo
     CDS             complement(2596..3595)
                     /locus_tag="ACH3L8_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1052 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DAZXUK010000308         3789 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZXUK010000308 DAZXUK010000000
VERSION     DAZXUK010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.308"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            21..1415
                     /gene="galP"
                     /locus_tag="ACH3L8_003800"
     CDS             21..1415
                     /gene="galP"
                     /locus_tag="ACH3L8_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFT4086706.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1492..1989
                     /locus_tag="ACH3L8_003801"
     CDS             1492..1989
                     /locus_tag="ACH3L8_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFT4086707.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            2084..2791
                     /gene="endA"
                     /locus_tag="ACH3L8_003802"
     CDS             2084..2791
                     /gene="endA"
                     /locus_tag="ACH3L8_003802"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFT4086708.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2871..3602
                     /gene="rsmE"
                     /locus_tag="ACH3L8_003803"
     CDS             2871..3602
                     /gene="rsmE"
                     /locus_tag="ACH3L8_003803"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFT4086709.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3615..>3789
                     /locus_tag="ACH3L8_003804"
     CDS             3615..>3789
                     /locus_tag="ACH3L8_003804"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311850.1"
                     /note="catalyzes the second step in the glutathione
                     biosynthesis pathway, where it synthesizes ATP +
                     gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate +
                     glutathione; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFT4086710.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLN"
BASE COUNT          874 a         1005 c         1021 g          889 t
ORIGIN      
        1 taactatatt ggagggcatc atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa
       61 tgacgttttt cgtctgcttc cttgccgctc tggcgggatt actctttggc ctggatatcg
      121 gtgtaattgc tggcgcactg ccgtttattg cagatgaatt ccagataact tcgcacacgc
      181 aagaatgggt cgtaagctcc atgatgttcg gtgcggcagt cggtgcggtg ggcatcggct
      241 ggctctcctt taaactcggg cgcaaaaaga gcctgatgat cggcgcaatc ctgtttgttg
      301 ccggttcact gttctctgcg gctgcgccaa acgttgaagt actgattctt tcgcgcgttc
      361 tgctggggct ggcggtgggt gtggcctctt ataccgcacc actgtacctg tcagaaattg
      421 cgccggaaaa aattcgcggc agtatgatct cgatgtatca gttgatgatc actatcggga
      481 tcctcggtgc ttatctttct gataccgcct tcagctacac cggtgcatgg cgctggatgc
      541 tgggtgtgat tatcatcccg gcaattttgc tgctgattgg tgtcttcttc ctgccagaca
      601 gcccacgttg gtttgccgcc aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc
      661 tgcgtgacac cagcgcggaa gcgaaacgcg aactggatga aatccgtgaa agtttgcagg
      721 ttaaacagag tggctgggcg ctgtttaaag agaatagcaa cttccgccgc gcggtgttcc
      781 ttggcgtact gttacaggta atgcagcaat tcaccgggat gaacgtcatc atgtattacg
      841 cgccgaaaat cttcgaactg gcgggttata ccaacaccac cgagcaaatg tgggggacag
      901 tgattgtcgg cctgaccaac gtacttgcca cctttatcgc aatcggcctt gttgaccgct
      961 ggggacgtaa accaacgcta acgctgggct tcctggtgat ggctgctggc atgggcgtac
     1021 tcggtacaat gatgcatatc ggtatccact ctccgtcggc gcagtatttc gccatcgcca
     1081 tgctgctgat gtttattgtc ggttttgcca tgagtgccgg tccgctgatt tgggtactgt
     1141 gctccgaaat tcagccgctg aaaggccgcg attttggcat cacctgctcc accgccacca
     1201 actggattgc caacatgatc gttggcgcaa cgttcctgac catgctcaac acgctgggta
     1261 acgccaacac cttctgggtg tatgcggctc tgaacgtact gtttatcctg ctgacattgt
     1321 ggctggtacc ggaaaccaaa cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag
     1381 gtcgtaaact gcgcgaaatc ggcgctcacg attaatctcc tcaggcttcc tcccgtcgcg
     1441 gaggaagccg cctcttgcag tcatcttttc ttcgctctat cctctgccgc tatgaaaaca
     1501 tcccgtctcc ctatcgccat ccaacaggcc gttatgcgcc gcctgcggga aaaactcgcc
     1561 caggccaacc tgaagctagg gcgtaactac ccggagccaa aactctctta cacccagcgc
     1621 ggaacctccg ccggaacagc ctggctggaa agctatgaaa tccgcctcaa tcccgttttg
     1681 ctgttggaaa acagtgaagc ttttattgaa gaagtggtac cgcacgaact ggcacatttg
     1741 ctggtatgga aacatttcgg ccgcgtagcg ccacatggca aagagtggaa gtggatgatg
     1801 gaaagcgtgc tgggcgttcc cgcccgtcgt acgcatcagt tcgaactgca atccgtgcgt
     1861 cgcaacacct tcccctaccg ctgtaagtgc caggaacatc agcttaccgt acgccgccat
     1921 aatcgcgtag ttcgtggcga ggccatctat cgctgtgttc actgcggtga acagctggtt
     1981 gcgaaataac cacctgaact atcaggaact ttcctgatct ggctgattgc ataccaaaac
     2041 aactttcgct acgttgctgg ctcgttttaa cacggagtaa gtgatgtacc gttatttgtc
     2101 tattgctgcg gtggtactga gcgcagcatt ttccggcccg acgttggccg aaggtatcaa
     2161 tagtttttct caggcgaaag ccgcggcggt aaaagtccac gctgatgcgc ccggtacgtt
     2221 ttattgcgga tgtaaaatta actggcaggg caaaaaaggc gttgttgatc tgcaatcgtg
     2281 cggctatcag gtgcgcaaaa atgaaaaccg cgccagccgc gtagagtggg aacacgtcgt
     2341 tcccgcctgg caattcggtc accagcgcca gtgctggcag gacggtggac gtaaaaactg
     2401 cgctaaagat ccggtctatc gcaagatgga aagcgatatg cataacctgc agccgtcagt
     2461 cggcgaggtg aatggcgatc gcggcaattt tatgtacagc cagtggaatg gcggcgaagg
     2521 ccagtacggt caatgcgcca tgaaggtcga tttcaaagaa aaagctgccg aaccaccagc
     2581 gcgtgcacgc ggtgccattg cgcgcaccta cttctatatg cgcgaccaat acaacctgac
     2641 actctctcgc cagcaaacgc agctgttcaa cgcatggaac aagatgtatc cggttaccga
     2701 ctgggagtgc gagcgcgatg aacgcatcgc gaaggtgcag ggcaatcata acccgtatgt
     2761 gcaacgcgct tgccaggcgc gaaagagcta acctacacta gcgggattct ttttgttaac
     2821 ccctacccca cgcgtacaac cgcgtgggga gacgacgcgg atttttaact atgcgtatcc
     2881 cccgcattta tcatcctgaa ccactgacca gccattctca catcgcgctt tgcgaagatg
     2941 ccgccaacca tatcgggcgc gtactgcgca tggggccggg gcaggcgttg caattgtttg
     3001 acggtagcaa ccaggtcttt gacgccgaaa ttaccagcgc cagcaaaaaa agcgtggaag
     3061 tgaaggtgct ggaaggccag atcgacgatc gcgaatctcc gctgcatatt cacctcggtc
     3121 aggtgatgtc gcgtggtgaa aaaatggaat ttactatcca gaaatcgatc gaactcggtg
     3181 taagcctcat tacgccactt ttttctgagc gctgcggcgt taaactggat agtgaacgtc
     3241 tgaacaagaa gcttcagcag tggcagaaga ttgcaattgc tgcctgtgag cagtgtggtc
     3301 gtaaccgggt gccggaaatc cgtccagcga tggatctgga agcctggtgt gcagagcagg
     3361 atgaaggact gaaactgaat cttcacccgc gcgccagtaa cagcatcaat acgttgcctt
     3421 taccggttga acgcgtccgc ctgctgattg gcccggaagg cggtttatcg gcagatgaaa
     3481 ttgccatgac tgcccgctat caatttactg atatcctgtt gggacctcgc gttttgcgta
     3541 cagagacaac tgcgctcacc gccattaccg cgctacaagt acgatttggc gatttgggct
     3601 aacggagaag aataatgatc aagctcggca tcgtgatgga ccccatcgca aacatcaaca
     3661 tcaagaaaga ttccagtttc gctatgttgc tggaagcaca gcgtcgtggt tacgaacttc
     3721 actatatgga gatgggcgat ctgtatctga tcaatggcga agcccgcgcc catacccgca
     3781 cgctgaacg
//
LOCUS       DAZXUK010000309         3788 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZXUK010000309 DAZXUK010000000
VERSION     DAZXUK010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.309"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..102
                     /gene="ymgI"
                     /locus_tag="ACH3L8_003805"
     CDS             <1..102
                     /gene="ymgI"
                     /locus_tag="ACH3L8_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFT4086711.1"
                     /translation="ILNYLNNSERSLVKQILIEDEIIVCATYLIPDI"
     gene            104..448
                     /locus_tag="ACH3L8_003806"
     CDS             104..448
                     /locus_tag="ACH3L8_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFT4086712.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            452..787
                     /locus_tag="ACH3L8_003807"
     CDS             452..787
                     /locus_tag="ACH3L8_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFT4086713.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(844..3165)
                     /locus_tag="ACH3L8_003808"
     CDS             complement(844..3165)
                     /locus_tag="ACH3L8_003808"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFT4086714.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(3282..3491)
                     /locus_tag="ACH3L8_003809"
     CDS             complement(3282..3491)
                     /locus_tag="ACH3L8_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086715.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         1015 a          890 c          819 g         1064 t
ORIGIN      
        1 attttgaatt atctgaataa tagcgagcgt tctttagtta agcagatctt aattgaagac
       61 gaaattattg tgtgtgcaac ttacttaata ccggacatct aagttgaaga aaaaaatact
      121 tgccttcggg cttatctcag cattgttctg ctccacgcca gcgatggcgg atatgaaccg
      181 caccacgaaa ggtgcattgc taggcgcagg cgttggttta cttactggca atggtgttaa
      241 tggcgtactg aaaggtgcag ctgttggcgc tggtgttagt gcagtaacag aaaaaggccg
      301 cgacggtaaa aatgcgcgta aaggtgcaaa agtgggtgcc gccgttggtg ctgtcactgg
      361 cgttctgacc ggcaatggcc tcgaaggggc gattaaaggt gccgtgattg gcggtacggg
      421 tggtgcaatt ctgggtaaaa tgaaataagg aatgatgatg aaaaaatttg ctttactggc
      481 gggcctgttt gttttcgcac caatgacctg ggcgcaggac tacaatatta aaaacggttt
      541 gccgtcagaa acctatatta cttgcgcaga agccaacgaa atggcaaaaa ctgacagcgc
      601 acaggttgca gaaattgttg cggtaatggg taatgccagc gttgccagcc gtgatttaaa
      661 aattgagcaa tctccggaac tgagcgccaa agtggtagaa aaactgaatc aggtttgcgc
      721 taaagacccg caaatgctgt tgatcacggc aattgatgac accatgcgtg ctatcggcaa
      781 aaaataatat ttcatatgta ccgcgtcgat gaatacgacg cggtttttta gctaacggcg
      841 ggatcagaac ttatatttca ggcccaccat cactgcggtg tcattgtagc cattatcacc
      901 cagctgcacg cccacattcc cccagacgct ggccgctgga ttcaggttgc catttacgcc
      961 aagtttaatt tcccccaaat tactcacccc atcttgttcg actttcacac cgttcattga
     1021 gacagcaaaa tctttactgt tatggatcca gttcatttca acaaaaggtt caaagtcgtc
     1081 accgtgcgga ccgctgtttt tctcctgagt acgaataaag gtgcggaagc cgagtcgggt
     1141 ctggatgttg ttatttgcgt cattctcaat acgtgagcca ttggcttccg tgtgcgcatt
     1201 atgatcgacg cccatccaga tagcctgtgc ctgtggctgg aagatccacg tatatttcgc
     1261 cgcttccaga ccaaaggatt tattcagggc aaaaccgtaa cccgcttcca gcgagacggt
     1321 tgcacctttc gcgctatagg attcataggc taattcatcc cctttcacgg agtttttaaa
     1381 ccagctatat tgcgcccagg agtcaatgta tgcgcctttt ttactgatgt catcggcaaa
     1441 ccaagtggca tacagccctg cgctatagcc tctgacactc cctttcgaac gataatccga
     1501 cacgctggaa tgagttaagt tgtagtcgcg ggcataacca gccatcactc ccaaacgcca
     1561 actgtcacta tcggtaaaac caccggttaa cagatcgccc cctaactgcg agacgtagcg
     1621 atgcgaggtc gttctcaact gtccgttgct gtcacgccag gcattatgcc cgccaatttg
     1681 acgtagccaa agtcggcttg aacgctcctg ttcagttaca ggatcgatgt agcgcgtttc
     1741 acccgcacgg tcgttcagac gcataacaaa aagagtgtta gcggctgcca ggttggcgac
     1801 gtaacttcca gcttcggggc gcaggacggg ggtaggcgca acagtaggcg ctgaacttcc
     1861 tccggagttt gtttcaccgc cgctcgttgc ctggaagtta gtcagatacc agtttttatt
     1921 ggtcgcttcg gtattacctc gggttaatga atattcgtag gcaccggcga aaatcctcga
     1981 atccttaata aattccccat ttgattcccc ctcaacgctg ataatctcta tcccatttaa
     2041 cgtgtaagct ccgctgcctc ctgcattggt aactttaact cgggttgttc cagaggtatt
     2101 tccattaata acgactttat ccgtagctga attatcatca tccaattcag ttctgagatg
     2161 caatacgcca ttattaccaa cgtaattttc agttattgtt aatcttgttg gttcgacatc
     2221 tcgtccatct gcccgggaaa tatagaccgt actattatca accgtgaggt tgctcaccgt
     2281 tgagtcagct ttaatatccc aggtactatt atctgaaagc gacagatagg tatgagtgtt
     2341 atcatctgtt gaaatattag ctgaacccgt tatttttgaa ttagttgcat cgacggtcaa
     2401 atgaccttta atattattga taaaaacatc tgcagccgat gatacctcac tatccgtaat
     2461 ggtcacgtta ctcgtctcat ctttatggta gaggatttca tttgctgacc gtagtgagct
     2521 accagataaa atatctaccg ttgcttcctg accatttatg gaagactcca gcacaattcc
     2581 agacccctga tgcgcgactg cagatgtatt tttcagcgtc accgtgccgc cattataaat
     2641 tttagcaatc gcggcgtcag gacccgttgt ataaatttct ccctggttaa atgaaactgt
     2701 cccctggcgg gcagcgatgg catgagcatt gtccccatgc gtggtgatat caacatcggt
     2761 taatgtaacg ctggcattgg tataatcaag cgtgtcacca gccttgacga cagtgccatt
     2821 agcgtacgcg gcataacttt ctgttccgtg ggttgtgatc tccccttttg ccatatccag
     2881 ttttgctatg gcattatttt cattacttcc tgtatcggca gtactttctt catcaagaga
     2941 agatacacac gcacacgcgc ttccgggaga tgcacacgca agaacgccga ctgcgtgatt
     3001 acccgcagta acaataaatg tatcatctcc gtcagaaaaa gagggggtaa ccgaaccgtc
     3061 agagttgact cttattatgg tgtcagtatt caggtttcca ctcgtttgac ttagcaaacc
     3121 aattgagtaa tcccccgttg tgacaatcgt gtttttaccc tgcaaaataa cagcaccgcc
     3181 aaattcactt acgattgcgc tagctgttaa tgctgggctt agaggactat tgtcagaagg
     3241 atataaaccg cccgttgtta caatgacgcc attgttaatg gttaacttac taccctctcc
     3301 tgcaacggat acccgtaact tccattaccc gttgttataa ctagcgcatt atcaatatct
     3361 tttacttttc catcttttat tgccacagca tgtttatatt catcgtgctg aataaactgt
     3421 tcagcatatg cattacccac cgccagagca ataagtccca aactaaaccc ggggagtttt
     3481 ttcagtttca tagattaact atccattcat tggattaaca aacattcctg gtcaaataac
     3541 ctatgagctc agattaccaa aacggagggt aaagacaaaa acaaaagcac aaaaaattta
     3601 aatagataaa cagcgacaat atataaatga taaataagtc ggcattatca tttttcacct
     3661 tattttcatg acattgatca ctttggggtg actattttgt ttaacaaata actcttcgac
     3721 atcaaaatta ttaatagttg ccatggtaat tatttcggtg atatataaaa acaactgtga
     3781 taacacca
//
LOCUS       DAZXUK010000310         3786 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZXUK010000310 DAZXUK010000000
VERSION     DAZXUK010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.310"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            6..260
                     /gene="yoeB"
                     /locus_tag="ACH3L8_003810"
     CDS             6..260
                     /gene="yoeB"
                     /locus_tag="ACH3L8_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFT4086716.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            343..1167
                     /locus_tag="ACH3L8_003811"
     CDS             343..1167
                     /locus_tag="ACH3L8_003811"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFT4086717.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            1213..2196
                     /locus_tag="ACH3L8_003812"
     CDS             1213..2196
                     /locus_tag="ACH3L8_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFT4086718.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            2357..2419
                     /gene="yoeI"
                     /locus_tag="ACH3L8_003813"
     CDS             2357..2419
                     /gene="yoeI"
                     /locus_tag="ACH3L8_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFT4086719.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            2409..3767
                     /gene="plaP"
                     /locus_tag="ACH3L8_003814"
     CDS             2409..3767
                     /gene="plaP"
                     /locus_tag="ACH3L8_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFT4086720.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
BASE COUNT          890 a          894 c          998 g         1004 t
ORIGIN      
        1 attgagtgaa actaatctgg tctgaggaat catgggatga ttatctgtac tggcaggaaa
       61 cagataagcg aattgttaaa aagatcaatg aacttatcaa agatacccgc agaacaccat
      121 ttgaaggtaa ggggaaacca gaacccctga aacataattt gtcaggtttc tggtcccgac
      181 gcattacaga ggagcaccgt ctggtatacg cggttactga tgattcactg ctcattgcag
      241 catgtcgtta tcattattga acagattcat tcatagaatg aattgtcagt tttgatacgc
      301 tagcgtagcc ttatagatct aaggtgtatc aggagataac ggatgaaaaa ggtcgcaatt
      361 gtcgggttag ggtggttagg tatgccgctg gcgatgtcac tttcagcgcg aggctggcaa
      421 gtcaccggga gtaaaaccac acaagatggt gtcgaagcgg cccgaatgag tggcattgat
      481 agctatctgc ttcgcatgga gcctgagtta gtttgcgatt ctgacgatct ggatgccctt
      541 atggatgccg atgcgctggt cattacgctt ccggcacgtc gtagcggccc cggcgatgag
      601 ttctatttac aagcggtaca agagttagtg gatagcgcgc tggcccatcg tattccccga
      661 attattttta ccagctcaac atctgtctat ggcgacgcgc aaggcacggt taaagaaact
      721 accccgcgta atccggtaac caacagtgga cgagtattag aagaactcga agactggctg
      781 cacaatttac ccggtacttc ggtcgatatt ctgcgtcttg cgggcctggt cggaccggga
      841 cgtcatccgg gacgcttctt tgccggaaaa accgcccctg atggtgaaca tggtgttaat
      901 ttagtccatt tagaagatgt tattggcgct atcactctgt tgttacaggc acctaaaggc
      961 ggacacatct ataatatatg tgcgccagct caccctgcgc gtaatgtttt ctatccgcag
     1021 atggcccgtt tactggggct gcaacctccg cagttcagaa atagtctgga cagcggcaaa
     1081 ggtaagatta ttgatggcag tcggatttgt aatgaactgg gatttgaata ccagtatccc
     1141 gatccgctgg taatgccgtt ggagtaaacg atcacgccag caggacgccg catgcactgc
     1201 aagggggcgt gtatgaaacc actgctggat gtgttgatga tcctcgatgc catagaaaaa
     1261 gagggcagtt ttgcggcggc gtcagccaaa ctctacaaga cgccgtccgc tctgagttac
     1321 accgttcaca agctggaaag cgacctaaat attcaattgc ttgatcgtag cggacaccgc
     1381 gctaaattca cccgcaccgg aaaaatgtta ttagagaaag ggcgggaagt tctgcatacc
     1441 gtgcgagaac tggaaaagca ggcgatcaaa ctgcatgaag gctgggaaaa cgagctggtg
     1501 attggcgtgg acgacacgtt tcctttttct ctgcttgcgc ctctcattga agctttttat
     1561 caacatcata gcgtgacgcg cctgaagttt atcaatggcg tactcggtgg ttcctgggat
     1621 gccctgactc aggggcgagc ggatattatc gttggcgcga tgcatgagcc accatcttcc
     1681 agtgagtttg gtttctcgcg gttgggcgat cttgaacaag tctttgcggt cgcgcctcat
     1741 catccgttag cccaggaaga agaaccgtta aaccgtcgta tcattaaacg atatcgggcg
     1801 attgtggtgg gggatactgc gcaggcaggc gcttctacgg cctcacaact tctcgacgaa
     1861 caagaagcca ttaccgtttt cgattttaaa accaaactgg agctgcaaat tagcggcctt
     1921 ggctgcggct atttaccacg gtatctggcg caacgttttc tcgatagtgg cgcgttaatc
     1981 gagaagaaag tggtcgccca aactctcttt gaacctgtct ggattggctg gaatgaacag
     2041 accgcagggc ttgccagcgg ctggtggcga gatgaaattt tagcaaatag tgcgatcgcc
     2101 ggtgtttatg caaaatctga tgacggaaaa tcagccattc aaagaaaaat tattatgaca
     2161 agcctctcat tctcttgtca tttccccccc atttaggcac aatgcgccgc tgtcaaaaaa
     2221 tgattaaaaa ccgacgttta attagcgtcg gttatttttt gcttcaaacc aatcattcat
     2281 accaagaggc cgggcttcgt accggataga tatttactaa aaatcgacag ttgttgtcgc
     2341 tgaggaatcc aaaaaaatgg ggcaattttt tgcttacgcg acggttatca ccgtaaagga
     2401 gaataaccat gtcgcataac gttactccaa acacctctcg cgtggaattg cgtaaaacgc
     2461 ttacgttagt tccggttgta atgatgggtc tagcctatat gcagccgatg acgctgtttg
     2521 atacatttgg tatcgtttca ggcctcacgg atggtcatgt gccgacagcc tatgcgttcg
     2581 cattgattgc gatcctgttt acggctctga gctacgggaa gctggttcgc cgctatcctt
     2641 ctgctggctc tgcatacact tacgcccaga aatccattag cccgactgtc ggctttatgg
     2701 tgggttggtc ttctctgctc gactatctgt tcgcgccgat gatcaacatt ctgctggcga
     2761 aaatctattt tgaagctctg gtgccttcca ttccatcgtg gatgtttgtg gtggcgctgg
     2821 tggcctttat gaccgccttt aacctgcgta gtctgaaatc cgtagcgaac gtcaacactg
     2881 taatcgtcgt gttgcaggta gtgctgatcg cggtgattct gggcatggtt gtttatggcg
     2941 tatttgaagg tgaaggcgct ggtacgctgg cgagcacgcg tccgttctgg tctggcgatg
     3001 cgcatgttat cccgatgatt accggggcga caatcctgtg cttctccttt accggctttg
     3061 acggcatcag caatctgtcg gaagaaacca aagatgcaga gcgcgtgatc ccgcgtgcga
     3121 ttttcctgac cgcgctgatt ggcggcatga tcttcatctt tgcaacttac ttcctgcagc
     3181 tgtacttccc ggatatctct cgctttaaag atccggatgc gtcacagcct gaaatcatgc
     3241 tgtacgttgc gggtaaagcg ttccaggttg gcgcgctgat cttctccacc attaccgtac
     3301 tggcgtccgg tatggcggcg cacgcaggcg tagcgcgtct gatgtacgta atgggtcgtg
     3361 acggcgtgtt cccgaaaagc ttcttcggtt atgtacaccc gaaatggcgt actccggcga
     3421 tgaacatcat cctggttggc gcgattgccc tgctggcaat caacttcgac ctggtaatgg
     3481 caacggcgct gattaacttt ggtgcgctgg tggcgttcac ctttgttaac ctgtcggtca
     3541 tctcgcagtt ctggatccgt gagaagcgta acaagacgct gaaagatcac ttccagtatc
     3601 tgttcctgcc gatgtgtggt gcgctgaccg ttggtgcgct gtgggttaac ctggaagaaa
     3661 gctcaatggt tctgggtctg atctgggctg ctatcggtct gatttacctg gcttgcgtca
     3721 ccaagagctt ccgtaatccg gttccgcagt acgaagacgt agcataatca tcttttctaa
     3781 taataa
//
LOCUS       DAZXUK010000311         3783 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZXUK010000311 DAZXUK010000000
VERSION     DAZXUK010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.311"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..781
                     /locus_tag="ACH3L8_003815"
     CDS             <1..781
                     /locus_tag="ACH3L8_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFT4086721.1"
                     /translation="LTSHEPVIGIMGKSGAGKSSLCNALFQGEVTPVSDVHAGTREVQ
                     RFRLSGHGHSMVITDLPGVGESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDE
                     YFWRHILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRLFRP
                     VHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRGQAREQFTGA
                     VDRIFDTAESVCVASVARTVLRAVRDTVVSVARAVWNWIFF"
     gene            1153..>3783
                     /locus_tag="ACH3L8_003816"
     CDS             1153..>3783
                     /locus_tag="ACH3L8_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HFT4086722.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFS"
BASE COUNT          870 a          979 c         1137 g          797 t
ORIGIN      
        1 actcaccagt cacgaacccg tgattggcat tatgggtaaa agcggggccg gtaaatcctc
       61 actctgtaat gcactgtttc agggggaggt caccccggtc agtgatgttc acgccggcac
      121 ccgggaagtg cagcgtttcc gtctgagtgg ccatggtcac agcatggtta tcactgacct
      181 gcccggggtg ggcgagagcc gggacagaga tgcagagtat gaagccctgt accgtgacat
      241 tctgcctgaa cttgacctgg tactgtggct gattaaagcc gatgaccgtg ccctgtctgt
      301 ggatgagtat ttctggcgac acatcctgca ccggggacat cagcaggtgc tgtttgtggt
      361 gacgcaggcc gacaaaacgg agccctgcca tgaatgggat atggccggta ttcagccttc
      421 tcccgcacag gcacagaata ttcgcgaaaa aacggaggcg gtattccgtc tgttccggcc
      481 cgtacatccg gttgttgccg tatcggcccg caccggctgg gaactggata cgctggtcag
      541 tgcgctaatg acagcacttc ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga
      601 cgagctgcgc acggaatctg tccggggtca ggcccgtgaa cagtttaccg gtgcggtgga
      661 ccggatattt gacacggcgg agagcgtctg tgttgcctct gttgcccgta cggttctgcg
      721 tgccgtccgt gacacggtgg tctctgttgc ccgcgctgta tggaactgga ttttcttctg
      781 aacagtctgc cagggaaatg cctgataaca ttacgttaac caactgaaaa catgacacaa
      841 acccctcata ctcctcctgt ttctcttgcg tgaccgcctt gtcgttaata gaataaaact
      901 atcgataaaa cagatatcga tcgttgctat cgatcgttta tagtggttaa tggtctgcga
      961 aaaggcagct gaatctcttc atcatgcaga acggaattgc acacaacgga ctgatacttc
     1021 tctggcggat gagagagggg aagcatttat gtccgggaaa aaccacttta tctgaccatg
     1081 ctgttttgta cctgccggta tccacttttg tgggtaccgg cttttttatt caccctctgt
     1141 aaggaaaagc tgatgaaacg acatctgaac accagctaca ggctggtatg gaatcacatt
     1201 acgggcaccc tggtggtggc ttccgaactg gcccgctcac ggggaaaacg cgccggtgtg
     1261 gcggttgtgc tgtctcttgc tgctgtcaca tcagtcccgg cactggctgc tgacacgatt
     1321 gtacaggcgg gagaaaccgt gaacggcgga acactgacaa atcatgacaa ccagattgtt
     1381 ctcggtacgg ccaacggaat gaccatcagt agcggtctgg agtatgggcc ggataacgag
     1441 gccaataccg gcgggcaatg gatacaaaat ggcggtatcg ccaacaacac tactgtcacc
     1501 ggtggtggtc ttcagagagt gaatgccgga ggaagcgttt cagacacggt tatcagtgcc
     1561 ggaggcggac agagccttca ggggcaggca gtgaatacca ctctgaacgg cggtgagcag
     1621 tgggtacatg aaggcgggat tgcaacgggt accgtcatta atgagaaggg ctggcagacc
     1681 gtcaaatccg gcgcaatggc aaccgacacg gttgtgaata ccggcgcgga agggggacca
     1741 gatgcagaaa atggtgatac cgggcagttt gttcgcggaa atgccgtacg taccactatc
     1801 aatgaaaatg gtcgtcagat tgtggctgct gaaggaacag caaataccac tgtggtttat
     1861 gccggcggcg accagacggt acacgggcat gcgctggata ccacactgaa tggcggttac
     1921 cagtatgtgc acaacggcgg tacggcgtct ggcactgttg taaacagtga cggctggcag
     1981 attatcaagg aaggtggtct ggcggatttc accaccgtta accagaaagg caaactgcag
     2041 gtgaacgccg gtggtaccgc cacacatgtc accctgaaac agggtggagc actggttacc
     2101 agtactgcgg caacggttct tggcagcaac cgtctgggca atttcacggt ggaaaacggt
     2161 aaggctgacg gagttgttct ggagtccggc ggtcgcctgg atgtactgga aggccattca
     2221 gcgcagaaaa cacgggtgga tgacggcgga accctggcag tgtctgccgg tggtaaggca
     2281 acaggtgtca ccatgacatc cggtggtgcc ctgattgcag acagtggtgc cactgttgag
     2341 gggaccaatg ccagcggtaa gttcagtatt gatggcacat ccggtcaggc cagcggactg
     2401 ctgctggaaa atggcggcag ctttacggtt aatgccggag gactggccag caacaccact
     2461 gtcggacatc gtggaacact gacgctggct gccgggggaa gtctgagtgg cagaacacag
     2521 ctcagtaaag gtgccagcat ggtactgaat ggcgatgtgg tcagtaccgg cgatattgtt
     2581 aacgcagggg agattcgctt tgataatcag acgacaccgg atgccgcgct gagccgtgct
     2641 gttgcaaaag gcgactcccc ggtaacgttc cataaactga ccaccagtaa cctcaccggt
     2701 cagggtggca ccatcaatat gcgtgttcgc cttgatggca gcaatacctc tgaccagctg
     2761 gtgattaatg gtggtcaggc aaccggcaaa acctggcttg cgtttacaaa tgtcggaaac
     2821 agtaacctcg gggtggcaac ctccggacag ggtatccggg ttgtggatgc acagaatggc
     2881 gccaccacag aagaaggtgc gtttgccctg agtcgcccgc ttcaggccgg cgcctttaac
     2941 tacaccctga accgtgacag cgatgaagac tggtacctgc gcagtgaaaa tgcttatcgt
     3001 gctgaagtcc ccctgtatac atccatgctg acacaggcaa tggactatga ccggattctg
     3061 gcaggctccc gcagccatca gaccggtgta aacggtgaaa ataacagcgt ccgtctcagc
     3121 attcagggcg gtcatctcgg tcacgataac aacggcggta ttgcccgtgg agccacgccg
     3181 gaaagcagcg gcagctatgg cttcgtccgt ctggagggtg acctgctgag aacagaggtt
     3241 gccggtatgt ctctgacgac aggggtgtat ggtgccgcag gccattcttc cgttgatgtt
     3301 aaggatgatg acggttcccg cgccggcacg gtccgggatg atgccggcag cctgggcgga
     3361 tacctgaatc tgacacacac gtcctccggc ctgtgggctg acattgtggc acagggaacc
     3421 cgccacagca tgaaagcgtc atcggacaat aacgacttcc gtgcccgggg ctggggctgg
     3481 ctgggctcac tggaaaccgg tctgcccttc agtatcactg acaacctgat gctggagcca
     3541 caactgcact atacctggca gggacttacc ctggatgacg gccaggataa cgccggttat
     3601 gtgaagttcg ggcatggcag tgcacaacat gtgcgtgccg gtttccgtct gggcagccac
     3661 aacgatatga gctttggtga aggcacctca tcccgtgaca ctctgcgcga cagtacaaaa
     3721 caccgtgtga gtgaactgcc ggtgaactgg tgggtacagc cttctgttat ccgcactttc
     3781 agc
//
LOCUS       DAZXUK010000312         3721 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZXUK010000312 DAZXUK010000000
VERSION     DAZXUK010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.312"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1002
                     /gene="tsuA"
                     /locus_tag="ACH3L8_003817"
     CDS             <1..1002
                     /gene="tsuA"
                     /locus_tag="ACH3L8_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFT4086723.1"
                     /translation="QRGRFCLTGGFRDMYIAKNNRMFYALLIAISAQSVGVFALIQAG
                     LLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSWIALFTYMVMSA
                     VMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVLLVITLWVVMKELKKPKLKV
                     ATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGVTSPTANIL
                     QFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRAADAQTTLRSGLGGVLMGFG
                     ASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN"
     gene            1016..1243
                     /gene="tsuB"
                     /locus_tag="ACH3L8_003818"
     CDS             1016..1243
                     /gene="tsuB"
                     /locus_tag="ACH3L8_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFT4086724.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1286..2710)
                     /gene="sbcB"
                     /locus_tag="ACH3L8_003819"
     CDS             complement(1286..2710)
                     /gene="sbcB"
                     /locus_tag="ACH3L8_003819"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFT4086725.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            2892..>3721
                     /locus_tag="ACH3L8_003820"
     CDS             2892..>3721
                     /locus_tag="ACH3L8_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4086726.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     "
BASE COUNT          886 a          881 c          977 g          977 t
ORIGIN      
        1 cagcgtgggc gtttttgcct gactggtggt tttcgcgata tgtatatcgc gaaaaataat
       61 cgcatgtttt acgccttgct gattgcaatt tcggcacaaa gcgtgggggt ttttgcgtta
      121 attcaggcgg gcctactgac ttacgaagcc ggggcgttcc cgtggcttgg tactgttata
      181 ggtgggtatc ttttcgggct gggaattgtt ctggcgggtg gatgtgccac cgggacctgg
      241 tatcgcgcgg gtgagggatt gatcggcagt tggatcgcgc ttttcactta tatggtgatg
      301 agtgcggtga tgcgttctcc acacgccagt ggtttaaatc aaaccttgca gcactacact
      361 actgaacata actctattgc tgagactttt aatttgtctg tgtggccgtt ggttaccgtt
      421 ttgctggtga taacgctctg ggtggtgatg aaagagttga agaagccaaa actcaaagtc
      481 gcgaccttac cgccgcgccg aaccgggatc gctcatattc tgtttgaaaa acgctggcat
      541 ccctttgtca ctgctgtact catcggtttg attgcgcttt tagcctggcc cctgagtgaa
      601 gcaaccggac gcatgtttgg gttaggagtc acttccccaa cggccaatat tcttcaattt
      661 ctggtcgcgg gtgacgtgaa atacattaac tggggcgttt tcctggtgtt agggatcttc
      721 gtggaggcat ttattgcagc caaagcgagc cgtgagttcc gcgttcgcgc agctgatgca
      781 caaacaacat tacgtagcgg gttaggtggt gtactgatgg gcttcggtgc cagtattgca
      841 ggtggttgct ctatcggtaa tggactggtt atgactgcaa tgatgacctg gcagggctgg
      901 attggccttg tatttatgat tctcggagtc tggactgcgt cctggcttgt gtatgttcga
      961 ccgcagcgta aggcgcgact ggcaactgct gcggcaaatt aagtaaggtg taaagatggc
     1021 gattaaaaag ctggatgtgg tgacgcaggt ctgtccgttt ccactcatag aagcaaaagc
     1081 agcactggca gaaatggcca gcggcgatga gttggtaatt gagtttgatt gcacccaggc
     1141 aactgaagca atcccacagt gggcggcaga agaaggacat gccatcaccg attatcagca
     1201 gattggcgat gcagcgtgga gcatcaccgt tcaaaaagcc tgactcaaca ttgtcctccg
     1261 ccgtaccagc ggcggcggct tcaaattaga cgatctcttc cgcgtactgc caaagtgctt
     1321 ttaacagcgc cactttctct ttgtcatcgg catattgttg taccagcatc tgcaattcat
     1381 cagcataacc ctgcaaaaac tctggcgtga agacctggtg gcggtgctcc agccagcgtt
     1441 gctgctcggc ataatccagc gtccccggga agttgcgtgc ccgataattg aacaacaact
     1501 tttcaattcg tttatcaaca aaagtgatat ccagtgccgg taaattacgc ggctcggttt
     1561 ccagcacaat tttcattgct gcacgatcgg cgtcactgaa aaagccatta taaagctgtg
     1621 catccacatt atctgaaggt gtaaacggtt cggcttccgc gaatatcgct accacttttt
     1681 cacgcacttg cggattttca cgcagaattt ttaggttatc gaggcaatgc tgacgattaa
     1741 ttcccagtcg gtcggcatct tccgggcgta gcgtattcgc ctgggccagt accggacatt
     1801 tattgatatg caccagctta accggaacgg cggcgttatc gccaagatcg gcttttgcgg
     1861 tatataaacg ctcgcgcaat gtgtcgatat ccagctccag taatggcgaa atgtctcctg
     1921 ccaaatccgc cataattacg gcattgcgat tttcaggatg ccacgccagc ggtgccaccc
     1981 agctggtatt gccacgccat gccccaaaca ttccggaaac gtgagccagg ggtttcatct
     2041 gcggaacatc aatcaacgcc atcagtttgt gtttattacg atgggtaaag agataatcaa
     2101 acaggcgtgg ctgacgcgtt tttaccagtt tcgccatcgc aatagtggcg tacacatcag
     2161 ccatcgcatc gtgggcgttg ctatgttcaa taccattcgc tttggttaaa tgctcaaggc
     2221 gaaagctcgg tagaccgtca tcattttcag gccagtttat tccttccggg cgcagggcat
     2281 aacaggcacg cataacatcc agtaaatccc agcgcgagtt atcatgctgc cagctccagg
     2341 cgtaaggatc gtagaaatta cgataaaaaa cgttgcgtgt gacttcgtcg tcgaaacgca
     2401 cattgttgta gcccagaata caggtcttcg gtacggtaaa aagcgagtga atacgggcgg
     2461 caaacgcggc ttcgttttct cctttcgccc gtgcctcctg cggggtaata ccggtaatta
     2521 atactgctcc aggccggggt aaatagtcat ccgcgggctt gcagtaaaag acttcgggtt
     2581 cgccgatgac attgaattcg tcatcggtgc gaatggctgc gaactgtgca gggcgatcta
     2641 acgcggggtg cgtgccaaag gtttcgtaat cgtgaaagag gaaggtaggt tgtttatctg
     2701 tgtctgtcat tgtctagcat tgtccactgt agttaattaa ctgtctgtta taactcgaaa
     2761 agcaaccctt ttttcacctg agtatgtcta cgaacgtcaa atatagttgt ttactgtgcg
     2821 ctgttgtcca tgtgatattg tgtaccagat gtgtgccaaa ctattatttc tgagcgtacc
     2881 aaatattatt tatggctata agcgacacaa aactgcgtac tatttatggt aaaccatatt
     2941 cgggcccaca agaagtggct gatgccgatg gcctcagcgt acgaatttca ccgaaggggg
     3001 tcatccagtt ccagtaccgc tatcgctggc atggcaagcc taatcgactt gggcttggtc
     3061 gatacccatc cctgtctttg aaggatgcca gacagatcac tgctgacttg cgaaagctct
     3121 atttctcagg aacggatcca cgcacctatt ttgaagagaa ggtggagaac tccatgacgg
     3181 tcgcccagtg tctcgactac tggttcgaca actacgtctc tacaactctc agagaaaaga
     3241 cccaggcact ttatcgatca acggttatga agcgcatgca tgacgccttc cctaatcgtc
     3301 cggcatcttc tatcacggtt aagcaatggg ttgacctgct taccgaagaa gaaaaagata
     3361 atccacgccg agcaaggcag gtgctaagtc aactaagatc agcaattagt tggtgcatgc
     3421 ggcgtcagtt gatagatagt tgcgcaatta tgagcatcca gccaagggac ttcggctccc
     3481 gcgctgaggt aggggatcgg gtactgtcgt atcacgaact ggctaagatt tggcttgcta
     3541 ttgaaagaag ccgtgcgtct acgtcaaata agctccttca tcagatgctt atgctgtggg
     3601 gggcgaggct ctcagagctt aggctggcaa caaagacaga atttgacctg ctggacaacg
     3661 tatggaccgt accgaaagag catagcaaga tgggtaatgt tatccgccgt ccaatcttcg
     3721 a
//
LOCUS       DAZXUK010000313         3708 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZXUK010000313 DAZXUK010000000
VERSION     DAZXUK010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.313"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            116..562
                     /locus_tag="ACH3L8_003821"
     CDS             116..562
                     /locus_tag="ACH3L8_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFT4086727.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            573..2024
                     /locus_tag="ACH3L8_003822"
     CDS             573..2024
                     /locus_tag="ACH3L8_003822"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFT4086728.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2014..3084
                     /locus_tag="ACH3L8_003823"
     CDS             2014..3084
                     /locus_tag="ACH3L8_003823"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFT4086729.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            3084..>3708
                     /locus_tag="ACH3L8_003824"
     CDS             3084..>3708
                     /locus_tag="ACH3L8_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFT4086730.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT          830 a          968 c         1007 g          903 t
ORIGIN      
        1 aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc
       61 acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc
      121 agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct
      181 gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac accagttcct
      241 gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg gtgtcgcagt
      301 gccgcacggt aaaagcgcct gcgttaaaca accgttcgta ttattcgccc gcaaagcgca
      361 ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt
      421 gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc gcaaaattat
      481 tcaccaggaa tttattcatc aactgcaaca gggcgatacc gaccaggtgc tggccttgtt
      541 aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttacgt attgtcgcga
      601 tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga
      661 aagcccgttc tctcggccat accataaaag tggaaactca agggtccagt ggcgttgaaa
      721 accgcttatc cagcgaagag attgccgctg ccgattacgt cattcttgct accgggcgtg
      781 gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag attgccatct
      841 ccctggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac tcgcagcttt
      901 ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc agcgtgatga
      961 gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg
     1021 tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg
     1081 cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc tttatgatcc
     1141 ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt
     1201 ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat
     1261 tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgcaaag
     1321 tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg ttcgttactc
     1381 tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc gacctcatgg
     1441 gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg gcagcgtttc
     1501 tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc gcctggttct
     1561 tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc ggcgttgtcg
     1621 cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc
     1681 gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac
     1741 cggctatacc ttacgctctg gcagctccgt tgccgatgat taccgccaat acgctggcgg
     1801 gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg
     1861 gtatctttga cccgctgatc ggcctgatgt cgccggtagg ttcgttttat ctggtgctgg
     1921 cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc
     1981 gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg
     2041 tgcgaagcca gcgccgttag cggcgatgaa caggaagttc gcgacatcct gataaacacg
     2101 ctggaacctt gcgttaatga aatcaccttt gatggtctgg gcagctttgt tgcccgtaag
     2161 ggaaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc
     2221 acccacatcg acgagagcgg ttttctgcgt ttcaccacca ttggtggctg gtggaatcag
     2281 tcgatgctca accatcgggt aaccatacgc acacacaagg gagtgaaaat ccctggtgtg
     2341 attggtttcg tcgcgcctca tgcgttaacg gaaaagcaaa ggcaacagcc gctgtcattt
     2401 gatgagatgt tcattgatat tggcgcaaac agtcgcgatg aggtggaaaa acgcggtgtg
     2461 gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga agataaagtg
     2521 gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact attgcagacg
     2581 gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga agttgggcta
     2641 cgcggggcac aaacctcggc ggaacacatt aaaccggatg tagtgatcgt gctggatacc
     2701 gctgtcgcag gcgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag
     2761 gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtagcagcg
     2821 ttaaaaaact gtgccgcaca taacgattta ccgctgcaat tttccaccat gaaaaccgga
     2881 gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc
     2941 ctgccaaccc gctatctgca cgctaacagc ggcatgattt caaaagccga ttacgatgct
     3001 ctgctcacgt tgatacgggg ttttctgacg accttaaccg cggagaaagt caacgcgttt
     3061 agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc
     3121 tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt tcaggcagga
     3181 ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcattttcg
     3241 ccagtggcag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc
     3301 tgcaaaagca cgataacgac gatcggctgc tggcgctgtt attactgaat actttcactc
     3361 cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtagca gagcgtctgc
     3421 cccagttaaa acagcgttat gaacgcactt gttgcctggc cagccgccct ggtttgggcc
     3481 atttcattga tgagacagaa gagaaactcg ttatcttgct ggcgaacttg ctgcgcaaag
     3541 atccgttttt aattccgctg gcgggcataa cacgagacaa ccttcagcat ttatccacgg
     3601 cctgcgacaa ccaacaccgc tggccgctca tacagggtga ttatctctcc agcctgattc
     3661 tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaat
//
LOCUS       DAZXUK010000314         3706 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZXUK010000314 DAZXUK010000000
VERSION     DAZXUK010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.314"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..211
                     /locus_tag="ACH3L8_003825"
     CDS             <1..211
                     /locus_tag="ACH3L8_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFT4086731.1"
                     /translation="RGGLEIQSQFLRGNEQVYQCQVAFVLPGERVMMAFTYARTTPLT
                     PADMTRWAEIKKNLRFRMRQEVRTN"
     gene            230..3616
                     /locus_tag="ACH3L8_003826"
     CDS             230..3616
                     /locus_tag="ACH3L8_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFT4086732.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
BASE COUNT          893 a          887 c         1205 g          721 t
ORIGIN      
        1 acgaggcggt ttggaaatac agagccagtt tctccggggt aacgagcagg tgtaccagtg
       61 tcaggtggca tttgtattgc cgggtgagcg ggtcatgatg gccttcacct acgccagaac
      121 cacaccgctg accccggcag atatgacgcg ctgggctgaa attaaaaaga atctgcgctt
      181 caggatgcgg caggaagtca ggacgaatta atcatacagg gaacgggaca tggaagaggc
      241 attctgggcg gcaagacagg gtgatgccct gctgcatacg tcgtttatgg cagatgtgct
      301 gggggcggtg ctggaggtgg ccgcaaatgt cgttattgat gccctcattg tcggcacaac
      361 ctcgttactg gcgacgtcag tggggatcac gatggggtgc acggccgtcg tgctgaccgg
      421 ttttgtggcg ggtgtggcga tggtgtacac cggcgtgaca gaaaaggtca gcgaagcctg
      481 ttcagccctc gcaaatatgc ttttcccgcc tcagattgaa ggttacatcc tcaccggctc
      541 tggtgacaca tggatcaaca gcaaacctgc cgcgcgggca gcggcgactg cggcatcgcg
      601 gcaggacata gaggcgcagg aagcgcaggc gaaggcagaa caggaagaag cgcagcgtca
      661 ggaggatgcc agaacgttcc gggatgtggc cggggaatat ctggaaatgg cgggggccat
      721 agccctggcg gttaacccgg tgacagggcc tgtgctgatg tcccgggctc tgcacgacca
      781 gctcagtacg gaagaagggc gttctgaaat ggtggatggc gtgacacatt ttgtgtcgga
      841 actatggcag cccactgtgg ccagtgcggc cccgggatcc accccgaccc ctgacgataa
      901 aatcgactgc cataaacatc cttcctcact gacgcagttt ctcgcacaaa aaaaagccgc
      961 ctttctggat gaccccctgg gaacggccct taacctgctg aatccgggcg gtatgctgga
     1021 aaccgccttt cagggagtga atgcggttat tggcagcatc agtaatctgt tcaaaggcga
     1081 tgacgagccg cccgcagcgg aatatatcgc ggaaggcgcg cgggatgtgc gtatcaacag
     1141 ccagccggcg acacgcagcg gggcacgctg tacctgcgag gccagggtgg tgaatgagcc
     1201 ggggaacggg gcgtttgtgt caccggatgt gcgcattggc ggtccgttac tggtggtcag
     1261 ggatatcaga agcggcaggt cgcagataac cctggtggca accgtggcgc tgatgtttct
     1321 tcgcccgggc aaaatgcttt ctaaaatcgc ctgttttgct gccagtgtcg ggatgagcat
     1381 gctgacgcag aaggccacca gtgcccttcc gcatccggtc aatgccgcca ccggggccaa
     1441 atacctggcc gatgacgatg acttcgattt cagtctgccg ggccactttc cgctggactg
     1501 gcagcgcgtg tacagcagtc gtgacgaacg tacggaagga atgtttggtc agggctggag
     1561 tgtaatgtat gaagtcagcc tcgtgtgcac accgggtagc gcagacgaaa actgcatgac
     1621 ctttgtgtcg ggcatggggc gacgcttgga tatggaagcc gtactgccgg ggggcgggtt
     1681 ttacagcccc ggtgaagggc tggcggtaag gcgtggtgag caggggcact ggcttatcag
     1741 cagcgatgac gggcagtgtt ttctgtttga ggctgacccg caccacccgc agcgccagcg
     1801 gctgaaaatg ctgggcgacc gtaacagcaa ctgtctgaat ctgtactacg acgaccgggg
     1861 ccgcattgtg gaaatcagcg gcgaacagca gcgtccgtgc atccgtctgt attacgaact
     1921 ggcggcgcat ccccggcggg tgacacagat ttatcagcat ttccctgaga ctgcaccttt
     1981 actgctgcgt cgttacagct atgacgaggc cggccacctg aacggcgtgt acgacagcac
     2041 ggggcacctg ctgcgtgagt ttgcttacga cgaaaaccac tgcatgacgc tgcaccgcca
     2101 gccgggcggc gaagggtatt attaccagtg gggctggtat gaaggtccgg atgacgcggc
     2161 atggcgggtg acggggcacc acacggacag cggtgaacag taccggctgg cgtgggacct
     2221 ggcgcagcgc aggctgtgcg tgacggacgg actggggcgc acacgttatc accagtggga
     2281 tgcgcagaat caggtgacgg cgtaccagga cgaagccgga caggtgacga cattccgctg
     2341 gagcgacgaa gaacgactgt tgctggggat gaccgaccct cagggcggca aatggcgcta
     2401 tgtgtatgac ctgcaggggc atatcacgga gacgcacgac ccgctggggc gggtggagca
     2461 ggcgcagtgg cacccggtgt ggcaccagcc ggaaacggag gtggacgcgg cgggaaacct
     2521 gctggggagg cgcgcggggg aaagggccac ggcagaaaac agcgtggtgc cgttcaaccg
     2581 gctgatgtct taccgtggtg tgcactaccg ttatgacgaa tttggccgtg cggtggaaaa
     2641 ggagggccgg agcggaacgc agagttaccg ttatgatgcg gaacaccgga tggtggaagt
     2701 gacgacagcg cgggggacat accggtatgt gtacgatgca ctggggaggc ggacggaaaa
     2761 acagcacatc agccctgacg gtaagccgta caaccggaca aaatttttat gggacggaat
     2821 gcggctggcg caggagagca ggccggaggg aacaggcagc ctgtacatct acagggacca
     2881 ggggagttat gaaccgctgg cgcgggtgga taaggcgggc aaagagggcc cgaaccggat
     2941 actgtatttt cacacggatg tgaacggcgc gccggaagag atgacggaca gcgacgggaa
     3001 aatcgtgtgg gagacggggt atcaggtctg gggtaacacg atacaggaga aggaccacgg
     3061 cggggtggag cagaacctgc gctaccaggg acagtacctc gacagggaaa cggggctgca
     3121 ctacaatctg cacaggtact atgatccgga tgtcgggcgg ttcatggtaa ctgacccgat
     3181 agggttgaga ggcggtttaa atctctacag ttatgcacct aatcctctaa aatatgctga
     3241 tccacttgga ttaactccat gtgctgttag taaccaaaaa gcgaatcgtt tattagatag
     3301 tagtgaaacc aaagttactg ttaggtctag aagtgacgct gaacaattgt ttatggatag
     3361 atatttaggt cataattata aaaatatgac aggtgagtca ggccccagca ctaagaactt
     3421 aatggaatat cttactgaaa ataaaacaaa agcaggttca tatcattggg atgatataaa
     3481 agatccttct gtaacaaaac cttcatatag agtgtcaggt catggaccag gaaatcctga
     3541 tggtgattta ccacatttac aagttcatca acatggtgga agtgttagac atatattttt
     3601 cccgtgggag acataataat gaacaatctc aatacattat taagtggaaa ggttttgctc
     3661 aaagaaatta gtccattaat gcctggatat agaacatgga ttgaaa
//
LOCUS       DAZXUK010000315         3698 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZXUK010000315 DAZXUK010000000
VERSION     DAZXUK010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.315"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..2199
                     /gene="ydeP"
                     /locus_tag="ACH3L8_003827"
     CDS             <1..2199
                     /gene="ydeP"
                     /locus_tag="ACH3L8_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFT4086733.1"
                     /translation="QMDIRHDVIAMFDMNKPEGFDCPGCAWPDPKNSASFDICENGAK
                     AIAWEVTDKQVNASFFAENTVQSLLTWGDHELEAAGRLTQPLKYDAVSDCYKPLSWQQ
                     AFDEIGARLQSYSDPNQVEFYTSGRTSNEAAFLYQLFAREYGSNNFPDCSNMCHEPTS
                     VGLAASIGVGKGTVLLEDFEKCDLVICIGHNPGTNHPRMLTSLRALVKRGAKMIAINP
                     LQERGLERFTAPQNPFEMLTNSETQLASAYYNVRIGGDMALLKGMMRLLIERDDAASA
                     AGRPSLLDDEFIQTHTVGFDELRRDVLNSEWKDIERISGLSQTQIAELADAYAAAERT
                     IICYGMGITQHEHGTQNVQQLVNLLLMKGNIGKPGAGICPLRGHSNVQGDRTVGITEK
                     PSAEFLARLGERYGFPPPHAPGHAAIASMQAICTGQARALICMGGNFALAMPDREASA
                     VPLTQLDLAVHVATKLNRSHLLTARHSYILPVLGRSEIDMQKSGAQAVTVEDSMSMIH
                     ASRGVLKPAGVMLKSECAVVAGIAQAALPQSVVAWEYLVEDYDRIRNDIEAVLPEFAD
                     YNQRIRHPGGFHLINAAAERRWMTPSGKANFITSKGLLEDPSSAFNSKLVMATVRSHD
                     QYNTTIYGMDDRYRGVFGQRDVVFMSAKQAKICRVKNGERVNLIALTPDGKRSSRRMD
                     RLKVVIYPMADRSLVTYFPESNHMLTLDNHDPLSGIPGYKSIPVELEPSN"
     gene            2447..2650
                     /gene="safA"
                     /locus_tag="ACH3L8_003828"
     CDS             2447..2650
                     /gene="safA"
                     /locus_tag="ACH3L8_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFT4086734.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            2718..3479
                     /gene="ydeO"
                     /locus_tag="ACH3L8_003829"
     CDS             2718..3479
                     /gene="ydeO"
                     /locus_tag="ACH3L8_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFT4086735.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1032 a          812 c          847 g         1007 t
ORIGIN      
        1 cagatggata tacgccatga tgttattgcc atgtttgaca tgaataagcc agagggcttt
       61 gactgtccgg gttgtgcatg gccagatcct aagaacagtg cgtcattcga catttgtgaa
      121 aacggcgcaa aagcaatcgc ttgggaagtc acggataagc aggtaaatgc ctctttcttt
      181 gctgagaata cggttcaatc attacttacc tggggagatc acgagcttga ggctgcgggg
      241 cgtctcactc agcctttgaa atatgatgcc gtcagcgact gttacaagcc attaagctgg
      301 caacaagctt tcgacgaaat tggcgcacgc cttcaaagct atagtgatcc caatcaggtt
      361 gaattctata cttcgggccg cacttccaat gaagctgcct ttctttatca gctttttgcc
      421 cgtgaatacg ggagcaataa ctttcccgac tgctccaaca tgtgccatga accgacaagc
      481 gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg tgttgctgga agactttgag
      541 aagtgcgatt tagtcatttg cattgggcat aaccctggta caaaccaccc tcgcatgctg
      601 acttcgttgc gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa tcctctacag
      661 gaacgtggcc tggagcgatt taccgcaccg caaaacccgt ttgaaatgct gacgaactct
      721 gagactcagt tggccagtgc ctactacaac gtgcgcattg gtggcgatat ggcgttgctc
      781 aaggggatga tgcgcctgtt aattgagcgc gatgatgctg caagtgccgc aggtcggccc
      841 tcattgcttg atgacgaatt tattcaaacg cataccgtcg gctttgacga gctacgccgt
      901 gacgttctca attccgagtg gaaagatatc gaacgtattt ctggactaag tcagacacaa
      961 atcgccgaac tggctgacgc atatgccgct gccgaacgca ccattatctg ttacggaatg
     1021 gggatcactc agcacgaaca tggtacccag aacgtgcagc aactggtcaa tttgctgttg
     1081 atgaaaggta acattggcaa gcctggtgcg ggtatctgcc cactacgtgg tcactctaat
     1141 gtacagggcg accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt tctggctcgc
     1201 ctgggtgagc gctatggctt ccccccacct catgcacctg gacatgctgc aattgccagc
     1261 atgcaagcaa tatgtacggg gcaggctcga gcattgatct gcatgggggg caactttgca
     1321 ctggcaatgc cagatcggga agcgagcgct gtaccgttaa cgcaattaga tttggcggta
     1381 cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg cacggcatag ctatattctg
     1441 ccggttctgg ggcgtagcga gattgatatg caaaaaagcg gtgcgcaggc ggtaaccgtt
     1501 gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc cggtgtaatg
     1561 ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc ccagagcgtg
     1621 gtagcctggg agtatctggt ggaagattat gatcgcattc gcaatgacat tgaagctgtg
     1681 ctgccagagt tcgccgacta taaccagcgc atccgtcatc ccggtggttt tcacctgata
     1741 aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt cattaccagc
     1801 aaagggctgt tagaagatcc ctcttcagcg tttaacagta agctggtcat ggcgacagta
     1861 cgcagccacg atcagtacaa cacgacgatt tatggtatgg atgatcgcta tcgaggggta
     1921 ttcggtcaac gagatgtggt ctttatgagt gctaaacaag ctaaaatttg ccgtgtaaaa
     1981 aacggcgaaa gagttaatct tattgcgctt acgccagacg gtaagcgcag ctcacgccgc
     2041 atggatagat taaaagtggt catttaccct atggctgacc gctcactggt gacctatttt
     2101 ccagaatcga atcacatgct aacacttgat aaccacgatc cattaagtgg tattcctggc
     2161 tataaaagta ttccggttga attagaacca tcaaattaat gcctcttctc atttcttctg
     2221 ctgtcatccg cacagcagaa gaattcctca ttgactatta ttgcgcaatt tgctcacatg
     2281 gattaaatta aactacatac tataagatat aaacttctgc ctacagctgt aagaaactcc
     2341 gctcagtact gaagcaccag tcctatttct tcttttctcc agcctgttat attaagcata
     2401 ctgattaacg acttttaacg ttatccgcta aataaacata tttgaaatgc atgcgaccac
     2461 agtgaaaaac aaaatcacgc aaagagataa ctataaagaa atcatgtctg taattgtggt
     2521 tgtcttatta ctgacactta cgttgatagc cattttttcg gcaattgatc agctgagtat
     2581 ttcagaaatg ggtcgcattg caagagatct tacacatttc attatcaata gtttgcaaga
     2641 ctggaaataa acagcaaatt ataaatgtga aatgtttaaa aggtatcgat aaaactttat
     2701 tgttttaagg agataaaatg tcgctcgttt gttctgttat atttattcat catgctttca
     2761 acgctaacat tttagataaa gattacgcct tctccgacgg cgagatcctg atggtagata
     2821 acgctgttcg tacacatttt gaaccttatg agcggcattt taaagaaatc ggatttaatg
     2881 aaaataccat taaaaaatat ctacaatgca ctaacatcca gacagtgacg gtgcctgttc
     2941 ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt gcttaatgaa atgattgctt
     3001 atctcaactc ggaagaacgc aatcatcata atttttcaga acttttgctt ttttcttgcc
     3061 tgtctatttt tgccgcatgc aaaggtttca ttacactatt aactaacggt gtgctatccg
     3121 tttccgggaa agtgagaaat attgtcaaca tgaagctggc gcacccatgg aagctgaaag
     3181 atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa gaaaaaacta aagcaagagc
     3241 aaacgacatt ctcacagatt ctattagatg ccagaatgca gcacgcaaaa aatttgatac
     3301 gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg ttatgccagt acatcttatt
     3361 ttatttatgc gttccgcaaa catttcggca acagtccgaa gagagtttct aaggagtacc
     3421 gttgtcaaag gcacacgggt atgaatacgg gcaacacgat gagtgcttta gctatttgat
     3481 tatttgctaa cgagtagtca accacgcacg ctacgtaaga attatatggg gcagccattc
     3541 cctgccccgc gttgttttta ggcgatatat ttattgaaat aaataagtga catccatcac
     3601 atatttatgc acttgcataa cctgttgcat gattatttat gatctcaatt cggcattttg
     3661 tcagtaaaat gcaataattt attaaatatc aataaatt
//
LOCUS       DAZXUK010000316         3631 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZXUK010000316 DAZXUK010000000
VERSION     DAZXUK010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.316"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(2..46)
                     /locus_tag="ACH3L8_003830"
     CDS             complement(2..46)
                     /locus_tag="ACH3L8_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086736.1"
                     /translation="MKRNDKILHYEGLN"
     gene            165..1889
                     /gene="ilvI"
                     /locus_tag="ACH3L8_003831"
     CDS             165..1889
                     /gene="ilvI"
                     /locus_tag="ACH3L8_003831"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFT4086737.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            1892..2383
                     /gene="ilvN"
                     /locus_tag="ACH3L8_003832"
     CDS             1892..2383
                     /gene="ilvN"
                     /locus_tag="ACH3L8_003832"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFT4086738.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVYVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            2395..2481
                     /locus_tag="ACH3L8_003833"
     CDS             2395..2481
                     /locus_tag="ACH3L8_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFT4086739.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            2563..3567
                     /gene="cra"
                     /locus_tag="ACH3L8_003834"
     CDS             2563..3567
                     /gene="cra"
                     /locus_tag="ACH3L8_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFT4086740.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT          842 a          875 c         1008 g          906 t
ORIGIN      
        1 atcaatttaa tccttcgtaa tggaggattt tatcgtttct tttcaccttt cctcctgttt
       61 attcttatta ccctgcgttt atgtctctgg ctgccaattg cttaagcaag atcggacggt
      121 taatgtgttt tacacatttt ttccgtcaaa cagtgaggca ggccatggag atgttgtctg
      181 gagccgagat ggtcgtccga tcgcttatcg atcagggcgt taaacaagta ttcggttatc
      241 ccggaggcgc agtccttgat atttacgatg cattgcatac cgtgggtggt attgatcatg
      301 tattagttcg tcatgagcag gcggcggtgc atatggccga tggcctggcg cgtgcgaccg
      361 gggaagtcgg cgtcgtgctg gtaacgtcgg gtccaggggc gaccaatgcg attaccggca
      421 tcgccaccgc ttatatggat tccattccat tagttgtcct ttccgggcag gtagcgacct
      481 cgttgatagg ttacgatgcc tttcaggagt gcgacatggt ggggatttcg cgaccggtgg
      541 ttaaacacag ttttctggtt aagcaaacgg aagacattcc gcaggtgctg aaaaaggctt
      601 tctggctggc ggcaagtggt cgcccaggac cagtggtcgt tgatttaccg aaagatattc
      661 ttaatccggc gaacaaatta ccctatgtct gccctgagtc ggtcagtatg cgttcttaca
      721 atcccactac taccggacat aaagggcaaa ttaagcgtgc tctgcaaacg ctggtagcgg
      781 caaaaaaacc ggttgtctac gtaggcggtg gggcaatcat ggcgggctgc catcagcagt
      841 tgaaagaaac ggtggaggcg ttgaatctgc ccgttgtttc ctcattgatg ggaatggggg
      901 cgtttccggc aacgcatcgt caggcactgg gcatgctggg aatgcacggt acctacgaag
      961 ccaatatgac gatgcataac gcggatgtga ttttcgccgt cggggtacga tttgatgacc
     1021 gaacgacgaa caatctggca aagtactgcc caaatgccac tgttctgcat atcgatattg
     1081 atcctacttc catttctaaa accgtgactg cggatatccc gattgtgggg gatgctcgcc
     1141 aggtcctcga acaaatgctt gaactcctgt cgcaagaatc cgcccatcaa ccgctggatg
     1201 agatccgcga ctggtggcag cagattgaac agtggcgcgc tcttcagtgc ctgaaatatg
     1261 acactcacag tgaaaagatt aaaccgcagg cggtgatcga gactctttgg cggttgacta
     1321 atggggacgc ttacgtgacg tccgatgtcg ggcagcacca gatgtttgct gcgctttatt
     1381 atccattcga caaaccgcgt cgctggatca attccggcgg tctcggcacg atgggttttg
     1441 gtttacctgc ggcactgggc gtcaaaatgg cgttgccaga agaaaccgtg gtttgcgtca
     1501 ctggcgacgg cagtattcag atgaacatcc aggaactgtc taccgcgttg caatacgagt
     1561 tgcccgtact ggtggtgaat ctcaataacc gctatctggg gatggtgaaa cagtggcagg
     1621 acatgatcta ttccggccgt cattcacaat cttatatgca atcactaccc gatttcgtcc
     1681 gtctggcgga agcctatggt cacgtcggga tccagatctc tcatccgcat gagctggaaa
     1741 gcaaacttag cgaggcgctg gaacaggtgc gcaataatcg cctggtgttt gttgatgtta
     1801 ccgtcgatgg cagtgagcac gtctacccga tgcagattcg cgggggcgga atggatgaaa
     1861 tgtggttaag caaaacggag agaacctgat tatgcgccgg atattatcag tcttactcga
     1921 aaatgaatca ggcgcgttat cccgcgtgat tggccttttt tcccagcgag gctacaacat
     1981 tgaaagcctg accgttgcgc caaccgacga tccgacatta tcgcgtatga ccatccagac
     2041 cgtgggcgat gaaaaagtac ttgagcagat cgaaaagcaa ttacacaagc tggtctatgt
     2101 cttgcgcgtg agtgagttgg ggcagggcgc gcatgttgag cgggaaatca tgctggtgaa
     2161 aattcaggcc agcggttacg ggcgtgacga agtgaaacgt aatacggaaa tattccgtgg
     2221 gcaaattatc gatgtcaccc cctcgcttta taccgttcaa ttagcaggca ccagcgataa
     2281 gcttgatgca tttttagcat cgattcgcga tgtggcgaaa attgtggaag ttgctcgctc
     2341 tggtgtggtc ggactttcgc gcggcgataa aataatgcgt tgagaatgat ctcagtgcgc
     2401 aatttgcagc ccaacatgtc acgttgggct ttttttgcga aatcagtggg aacctggaat
     2461 aaaagcagtt gccgcagtta attttctgcg cttagatgtt aatgaattta acccatacca
     2521 gtacaatggc tatggttttt acattttacg caaggggcaa ttgtgaaact ggatgaaatc
     2581 gctcggctgg cgggagtgtc gcggaccact gcaagctatg ttattaacgg caaagcgaag
     2641 caataccgtg tgagcgacaa aaccgttgaa aaagtcatgg ctgtggtgcg tgagcacaat
     2701 taccacccga acgccgtggc agctgggctt cgtgctggac gcacacgttc tattggtctt
     2761 gtgatccccg atctggagaa caccagctat acccgcatcg ctaactatct tgaacgccag
     2821 gcgcggcaac ggggttatca actgctgatt gcctgctcag aagatcagcc agacaacgaa
     2881 atgcgctgca ttgagcacct tttacagcgt caggttgatg ccattattgt ttcgacgtcg
     2941 ttgccacctg agcatccttt ttatcaacgc tgggctaacg acccgttccc gattgtcgcg
     3001 ctggaccgcg ccctcgatcg tgaacatttc accagcgtgg ttggtgccga tcaggatgat
     3061 gccgaaatgc tggcggaaga gttacgtaag tttcccgccg agacggtgct ttatcttggc
     3121 gcgctgccgg agctttctgt cagcttcctg cgtgaacaag gtttccgtac tgcttggaaa
     3181 gatgatccgc gcgaagtgca tttcctgtat gccaacagct atgagcggga ggcggctgcc
     3241 cagttattcg aaaaatggct ggaaacgcat ccgatgccgc aggcgctgtt cacaacgtcg
     3301 tttgcgttgt tgcaaggagt gatggatgtc acgctgcgtc gcgacggcaa actgccttct
     3361 gacctggcaa ttgccacctt tggcgataac gaactgctcg acttcttaca gtgtccggtg
     3421 ctggcagtgg ctcaacgtca ccgcgatgtc gcagagcgtg tgctggagat tgtcctggca
     3481 agcctggacg aaccgcgtaa gccaaaacct ggtttaacgc gcattaaacg taatctctat
     3541 cgccgcggcg tgctcagccg tagctaatcc gcgaacaaaa atacgcgcca ggtgaatttc
     3601 cctctggcgc gtagagtacg ggactggaca t
//
LOCUS       DAZXUK010000317         3613 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZXUK010000317 DAZXUK010000000
VERSION     DAZXUK010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.317"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(210..455)
                     /locus_tag="ACH3L8_003835"
     CDS             complement(210..455)
                     /locus_tag="ACH3L8_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFT4086741.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(589..1974)
                     /gene="puuP"
                     /locus_tag="ACH3L8_003836"
     CDS             complement(589..1974)
                     /gene="puuP"
                     /locus_tag="ACH3L8_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFT4086742.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(1964..2128)
                     /locus_tag="ACH3L8_003837"
     CDS             complement(1964..2128)
                     /locus_tag="ACH3L8_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086743.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(2277..>3613)
                     /gene="puuA"
                     /locus_tag="ACH3L8_003838"
     CDS             complement(2277..>3613)
                     /gene="puuA"
                     /locus_tag="ACH3L8_003838"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFT4086744.1"
                     /translation="RYLERYPNTQYVDVLLTDLNGCFRGKRIPVSSLKKLEKGCYFPA
                     SVFAMDILGNVVEEAGLGQEMGEPDRTCVPVLGSLTPSAADPEFIGQMLLTMVDEDGA
                     PFDVEPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRQRDAEGDLQPPCAPGTDDRNT
                     QSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYHTDNVLEACD
                     DALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENVLSDTEGEDS
                     PLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPCGD
                     RHNHRVEYHVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQEGLPFPIRQ
                     SDALGEFIENDHLRRYLGERFCHVYHACKNDELLQFERLITETEIEWMLKNA"
BASE COUNT          912 a          996 c          921 g          784 t
ORIGIN      
        1 cgttgaacag gtggcgtcgc catctgttca acattcgtac ccgtgatttc tctataacta
       61 taactcacag aacaacttag cgaggagcag agcggtacga tcgtgagaaa cccattaaag
      121 cacgctgcag tgcgtgtcat tgttagccgg atgcggcgtg aacgctttat ccggacaacg
      181 atactgaccg atcgtctgca attaaataat tactcattac cccattgatt caaaaactct
      241 gcgatctcat caatgcgtac gggattaatc cccgcttcag cagccatttc atgttgggct
      301 tcttcgctga tctcttcatt gttcatcaaa cgggtgagca gtaactggaa gtagtgggcc
      361 agcggtgtgc gttcagcctc tgcgacaggc tctgcgcatt cggtcgcgta ctcatcggca
      421 atatcaaaat attttagcgg gatatcgtgg ttcattattt gcccctgggg ttaatgcgct
      481 aagcataagg gcggatgata gcactcgtga tccgggtcat acatcttttt atagttaatg
      541 gcgcggcgcg ttaccctcag gcaggataat gcgccgcgca tccggctatt acgtttcgct
      601 caccggcgtt ctgtcacctt cgtacagcgg cactttgcga tagcggcgga tcagatacca
      661 caaatatgcg ccgcctaacg aagcccatac cagaccgagt gtcagtgacg ttgactctag
      721 gttaacccac agcacaccca ccgtcagcgc gccaaccagc ggcatcagca aatagtggaa
      781 gtgatccttc cagcttttat tcattccttt acgccgccag aaatgattaa acaccgacag
      841 attcacgaag gtaaacgcca ccagagcacc gaagttaatc agtgctgtcg cggtgactaa
      901 atcaaagaac agcgccgaca gcgcgacaat cccgaccata atgacgttga gtgccggagt
      961 ccgccatttt gggtgcacat agccaaacac gcgctccgga aacacattgt cacgccccat
     1021 cacatacagc agacgcgaca cgctggcatg tgaagccagg ccagacgcta atgtgttcac
     1081 aaacgtggtg cagaggaaaa tcgactgaaa gagcttgccg ccgacgtaca gcgcaatttc
     1141 aggcagtgcg gcgtccgggt ctttaaagcg gctgatatcg ggaaagaaca gctgcataaa
     1201 gaacgacgcc gcgataaaga taacgccgcc gtataccgcc gtcaggaaga tggctttcgg
     1261 gatcacgcgt gcggcgtccg gcgtctcttc cgaaagcgtg gtcaccgcat cgaaaccgag
     1321 gaacgagaaa cagacaatcg tcgcaccggt aataatcggg atcaggtgcg cgttctcgct
     1381 gataaacggc tgaagtgacc agacggtgcc aacgccttct cctttatgca gtccctgaac
     1441 caccaggaag ataaacacca ccatgatgga gatttgcacc agtacaaaca gggtattgaa
     1501 gttagcgacc aggttgacgc tcttcagatt cgcggcggtt aaaatggcga cgaaggttac
     1561 cacccacacc cacggcggca cttccgggaa gagggcggag agatagattt tcgccaacaa
     1621 aacgttgatc attggcaaaa agagataatc cagcagtgat gaccagccga ccataaatcc
     1681 gacgtgcggg ttaatcgact tttgcgcgta ggtataggcc gaaccggcct ccggaaactg
     1741 gcgaaccagt ttgccgtagc tgatagcggt aaacagcacg cccgccagcg ccagcaaata
     1801 ggacgccgga acgtgaccgt cgctaatgcc ggagacaata ccaaaagtat caaatacggt
     1861 catcggcgtg agataggcca gacccatcat taccacctgc cacaatttca gtgattttcg
     1921 cagacgggtt ttgccgggtt gcgcagcaat attcagtggt gaattaatag ccataattgc
     1981 tgcctcccct gcgtaataag gtttgcggtc gtgtcgacct gaaatcaaac ctgtttagca
     2041 agggaagcaa agggggcgga gtccagtcag tagcaggagg ggcgcatacg cagcctccct
     2101 cgccatgcgg ggatttaatc atcggcatca tctcatttcc tcgtcacagt cttaatcgtt
     2161 aacggaagcc cgttgccgcc tgcgctccat aacgcgacat ccgggaaatg tctggattac
     2221 cagcaaatgt caggcctggc tccgctcagg ccgatgaaac aaccccgcaa ggggaattac
     2281 gcgtttttca acatccactc aatttctgtt tctgtgatga ggcgctcaaa ctgcaacagc
     2341 tcatcatttt tacaggcgtg atacacatgg caaaaccgtt cgcctaaata gcggcgcagg
     2401 tgatcattct cgataaactc acccagggca tcgctctggc gaatcgggaa gggtaagcct
     2461 tcctgttcca gcccgttgcc ttcgacttct tcctgcaacg gcagctcgtt atcaaggcca
     2521 tgcaaaatac cggcaaaaat cgctgccatc accagatatg ggttggcatc ggcacccgcc
     2581 acgtgatatt ccacgcggtg attatgacgg tcaccgcacg gaatacgcag ggcgacggtg
     2641 cggttgttat ggccccacga cgcctgcgtc ggcacataca ttcccggctg gaagcggcga
     2701 tacgagttca cgtttggtgc cagcaacgcc atcgacgacg gcatcaggtc aatcatcccg
     2761 gcgagcatct ttttcagcag cggcgaatct tcgccttctg tgtcagaaag cacattctcg
     2821 ccacgattat tttgcatact gatatggata tgcatcccgc tgcccgcgtg ctcttcatac
     2881 ggcttcgcca taaaagtggc gtgcatctta tgcttttctg ccatcagacg cactaggcgt
     2941 tttagtgcca gcgcatcatc gcaggcttcc agcacgttat cagtatggta aaggttgatt
     3001 tcaaactgac ccggcgaggc ctcagcgacc gcgccatctg ccggaatcag ctgcaactgc
     3061 gccagttcat caatatcatt gagcacgtcg gcgaagtggt tgaggttatc aacggagtaa
     3121 acctggcttt gtgtattgcg gtcatcggtg ccaggcgcgc agggcggttg cagatcccct
     3181 tcagcgtcgc gctggcgatc cagtaaatag aactccagct ctaccgctac aaccgggaac
     3241 aatccgcgct ggcgcagctg ctgccacagg cggttaagaa cgttccgcgg ctcaacgtca
     3301 aagggagcgc catcttcatc gaccatggtc aggagcatct gaccgataaa ctctggatcg
     3361 gcggcagaag gagttaagga accgagaaca ggaacacagg tacgatccgg ctcgcccatt
     3421 tcctgaccca gacctgcttc ttctactacg ttgcccagaa tatccattgc aaataccgag
     3481 gccgggaaat aacacccttt ctcgagcttc ttcaggcttg aaacagggat acgttttcca
     3541 cggaagcaac cattcaaatc agtaagtaaa acatcaacat attgcgtatt cgggtagcgc
     3601 tccaggtagc gtt
//
LOCUS       DAZXUK010000318         3606 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZXUK010000318 DAZXUK010000000
VERSION     DAZXUK010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.318"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..217
                     /locus_tag="ACH3L8_003839"
     CDS             <1..217
                     /locus_tag="ACH3L8_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial assembly protein"
                     /protein_id="HFT4086745.1"
                     /translation="VNRSGNGLVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQS
                     VNIKSNNANNWYLTIINDHGNYISDKI"
     gene            233..2713
                     /locus_tag="ACH3L8_003840"
     CDS             233..2713
                     /locus_tag="ACH3L8_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFT4086746.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            2729..3606
                     /locus_tag="ACH3L8_003841"
                     /pseudo
     CDS             2729..3606
                     /locus_tag="ACH3L8_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
BASE COUNT         1056 a          671 c          880 g          999 t
ORIGIN      
        1 ggtaaatcgc agtggcaatg gattggtgat aaaaaatgac tcagctaatt gggtgacgat
       61 ttcggatgtc aaagctaata atgttaaagt caattatgaa actattatga ttgccccctt
      121 agaaagtcag agtgttaata tcaaaagtaa taatgcaaat aactggtatc tgaccattat
      181 caatgaccac ggcaactata ttagtgacaa aatttaattg caggagctgc ctatgttgag
      241 aatgacccca cttgcatcag caatcgtagc gttattgctc ggcattgaag cttatgcagc
      301 tgaagaaacc tttgataccc attttatgat aggtggaatg aaagaccagc aggttgcaaa
      361 tattcgtctt gatgataatc aacccttacc ggggcagtat gacatcgata tttatgtcaa
      421 taagcaatgg cgcgggaaat atgagattat tgttaaagac aacccgcaag aaacatgttt
      481 atcaagagaa gttatcaagc ggttaggcat taatagcgat aacttcgcca gcggtaagca
      541 atgtttaaca tttgagcaac ttgttcaggg tgggagctat agctgggata tcggggtttt
      601 tcgtctcgat ttcagtgtcc cgcaggcttg ggtggaagaa ctggaaagtg gctatgttcc
      661 accggaaaac tgggagcggg gtattaatgc gttttatact tcttattatg tgagtcagta
      721 ttacagcgac tataaagcgt cgggtaacaa caagagtaca tatgtacgtt ttaacagcgg
      781 gttaaattta ctggagtggc aactgcattc tgatgccagt ttcagtaaaa caaataacaa
      841 tccaggggtg tggaaaagca ataccctgta tctggaacgt ggatttgccc aatttctcgg
      901 cacgcttcgc gtgggtgata tgtacacatc aagcgatatt tttgattctg ttcgcttcag
      961 cggtgtgcgg ttgtttcgtg atatgcagat gttgcctaac tcgaaacaaa attttacgcc
     1021 acgggtgcag gggattgctc agagtaacgc gctggtaact attgaacaga atggttttgt
     1081 ggtttatcag aaagaggttc ctcctggccc gtttgcgatt acagatttgc agttggccgg
     1141 tggtggagca gatcttgatg ttagcgtgaa agaggcggac ggctcggtaa ccacctatct
     1201 ggtgccttat gcagcggtgc caaatatgct gcaacccggc gtgtcgaaat atgattttgc
     1261 ggcgggccgt agccatattg aaggggcgag caagcaaagt gattttgtcc aggcaggtta
     1321 tcagtatggt tttaataact tattgacgct gtatggcggc acgatggttg ctaataatta
     1381 ctatgcgttt accctcggaa cgggttggaa cacgcgcatt ggcgcaattt ccgtcgatgc
     1441 cacgaagtcg catagtaaac aagacaacgg cgatgtgttt gacgggcaaa gttatcaaat
     1501 tgcctacaac aaatttgtga gccaaacgtc gacgcgtttt ggtctggcgg cctggcgtta
     1561 ttcgtcgcgt gattaccgga catttaacga tcacgtttgg gcaaacaata aagataatta
     1621 tcgccgtgat gaaaacgata tctatgacat tgccgattat taccagaacg attttggccg
     1681 taaaaatagc ttttctgcca atatgagcca gtcattgcca gaaggctggg gttctgtgtc
     1741 cttaagtacg ttatggcgag attactgggg gcgtagcggc agcagtaagg attatcagtt
     1801 gagttattcc aataactggc ggcggataag ctataccctc gcggcaagcc aggcgtatgg
     1861 cgagaatcat catgaagaga aacgttttaa tatttttata tcaattccct gtgattgggg
     1921 tgatgacgtt acgacgcctc gtcggcaaat atatatgtct aactcaacga cgtttgatga
     1981 tcagggtttt gcctcaaata atacgggatt atcaggaaca gtagggagtc gggatcagtt
     2041 caattatggt gtcaacctga gtcatcaaca tcagggaaat gaaacgacag ctggggcgaa
     2101 tttgacctgg aacgcgccgg ttgcgacagt gaatggcagt tatagtcagt cgagtactta
     2161 tcgacagact ggagccagtg tttcaggggg cattgtcgcc tggtcgggtg gcgttaatct
     2221 ggcgaaccgt ctttccgaaa cgtttgctgt gatgaatgcg ccaggaatta aagatgctta
     2281 tgtcaatggg caaaaatatc gcacaacaaa ccgtaatgga gtggtggtat acgacggaat
     2341 gacaccttat cgggaaaatc acctgatgct ggatgtgtcg caaagcgata gcgaagcaga
     2401 attacgtggc aaccggaaaa ttgccgcccc ttatcgcggc gcggttgtac tggttaattt
     2461 tgataccgat cagcgcaagc cctggtttat aaaagcgtta agagcggatg gtcaaccatt
     2521 aacgtttggt tatgaagtca atgatatcca tggtcataat attggcgttg tcggccaggg
     2581 aagccagtta tttattcgca ccaatgaaat accgccatcg gttaatgtag caattgataa
     2641 gcaacaagga ctttcatgca caatcacctt cggtaaagag attgatgaaa gtagaaatta
     2701 tatttgccag taatagtcag gtggttctat ggaaattcgc ataatgctat ttatattaat
     2761 gatgatggtt atgcctgtga gctatgcggc atgttatagt gagttatctg ttcagcacaa
     2821 tttggttgtt caggggaatt ttgcacttac tcaaacacaa atagcgacat atgagcataa
     2881 ttttaatgat tcgtcatgcg tgagtacaaa tactatcacc cctatgagcc catcggatat
     2941 tattgttgga ctttataacg ataccattaa attaaattta cattttgaat ggaccaataa
     3001 aaacaacatc acgttgtcaa ataatcagag cagtttcacc agtggttatt cggtaacggt
     3061 gacacctgcg gccagtaatg caaaagtgaa tatttctgcg gggggcggtg gttcagtgat
     3121 gattaatggt gttgcgacat tatccagtgc ttcatcatcg acacgcggga gtgccgcagt
     3181 acaatttcta ctgtgtttat taggtggcaa gtcatggggt gcatgtgtaa atagctacag
     3241 aaatgcattg gcacaaaatg caggtgtcta ttccttcaat ctgacattgt catacaaccc
     3301 gataaccaca acctgcaaac aggacgattt attaattact ttagacagta ttcccgtttt
     3361 acaattacca gccacaggta acaaagcaac aataaatagt aaaaaaaggg atattattct
     3421 gcgttgtaaa aatttattag gtcaacaaaa tcaaacatca cggaaaatgc aggtgtattt
     3481 atcaagttct gacttgttaa ccaacagcaa cacaatactg aaaggtgcgg aagataatgc
     3541 gtaggattta ttcttgaaag taatggttcg ccagtcacac ttttaaatat cactaacagc
     3601 agtaaa
//
LOCUS       DAZXUK010000319         3598 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZXUK010000319 DAZXUK010000000
VERSION     DAZXUK010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.319"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..199
                     /locus_tag="ACH3L8_003842"
     CDS             <1..199
                     /locus_tag="ACH3L8_003842"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086747.1"
                     /translation="SDITYEYSNQLKIRRDFILRYLIWISVYLNNVKREFSQETLYKK
                     YKIQLSVAKDTISLAKKYYTT"
     gene            260..976
                     /gene="nanC"
                     /locus_tag="ACH3L8_003843"
     CDS             260..976
                     /gene="nanC"
                     /locus_tag="ACH3L8_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFT4086748.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            996..2102
                     /gene="nanM"
                     /locus_tag="ACH3L8_003844"
     CDS             996..2102
                     /gene="nanM"
                     /locus_tag="ACH3L8_003844"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFT4086749.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2167..3147
                     /locus_tag="ACH3L8_003845"
     CDS             2167..3147
                     /locus_tag="ACH3L8_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFT4086750.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3257..3462
                     /locus_tag="ACH3L8_003846"
                     /pseudo
     CDS             3257..3462
                     /locus_tag="ACH3L8_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         1075 a          751 c          825 g          947 t
ORIGIN      
        1 aagtgatata acttacgaat attcaaatca attaaaaatc agaagagatt ttatactcag
       61 gtatttaatc tggatctctg tttatttaaa taatgttaaa agagaatttt cacaggagac
      121 cttatacaaa aaatataaaa tacagttatc cgttgccaaa gacactataa gcctggcaaa
      181 aaaatattac acaacataaa tgctaattgt ttatgcgggc tttgtattgc tttctgtatc
      241 ctacaaatga gtgaaattta tgaaaaaggc taaaatactt tctggcgtat tattactgtg
      301 cttttcgtcc ccattaattt ctcaggctgc gacactggac gtacgtggtg gatatcgtag
      361 tggaagccac gcctatgaga ctcgactcaa agtcagtgag ggatggcaaa atggatggtg
      421 ggcaagcatg gaaagtaata cctggaatac cattcatgat aataaaaagg aaaatgccgc
      481 actcaatgat gttcaggttg aagttaatta cgcaattaaa cttgatgatc aatggacggt
      541 gcgcccggga atgttaacgc attttagcag caacggcaca cgctacggac cctacgtaaa
      601 actgtcctgg gacgcgacaa aagatcttaa ttttggcatt cgctatcgtt acgactggaa
      661 agcttaccga caacaagact tatccggtga tatgtctcgt gataacgttc atcgttggga
      721 tggatatgtc acttaccata ttaatagtga tttcaccttc gcatggcaaa cgacgctata
      781 cagcaaacag aatgattatc gctatgcaaa ccacaagaaa tgggcgacgg aaaatgcctt
      841 tgttctacaa taccatatga cgcccgatat tacgccatac atagaatatg actaccttga
      901 ccgccagggt gtttacaacg gcagagataa tttatcggaa aacagttatc gcattggtgt
      961 gtcatttaaa ctgtagtaga caggagacag tcacaatgaa taaaactata atggcgcttg
     1021 ctatcatgat ggcttcattt gccgcaaacg cgtctgtatt accggaaact cctgtgccat
     1081 ttaaaagtgg taccggagca attgataacg acactgtcta cattggttta ggtagcgcag
     1141 gtacagcatg gtacaagcta gatacacagg ccaaagataa aaaatggact gcgttagctg
     1201 cattccctgg tggaccgaga gagcaagcaa cctctgcatt tattgatggc aatctgtatg
     1261 tgtttggcgg cattggcaaa aacagcgagg gattgactca ggtatttaat gacgtacaca
     1321 aatacaaccc caaaaccaat agctgggtta aattgatgtc gcacgcgccg atgggcatgg
     1381 cgggtcatgt gacttttgta cacaacggca aggcttatgt tactggcggt gttaaccaga
     1441 atatcttcaa tggctatttt gaagatctca acgaggctgg aaaagattca accgctatag
     1501 ataaaatcaa cgcccactat tttgacaaaa aagcagaaga ttatttcttc aataagtttc
     1561 tgttgtcttt tgatccctca acacagcaat ggagttacgc tggcgaatcg ccctggtacg
     1621 gaacggctgg tgcggcggtt gtgaataaag gtgataaaac ctggcttatt aatggcgaag
     1681 ccaaaccagg attgcgaacg gatgccgtat ttgaacttga tttcaccggt aataatttaa
     1741 aatggaataa gcttgatccc gtctcatcac cagatggcgt cgctggcggt tttgcgggga
     1801 taagcaatga ttctcttata tttgctggag gggccggatt caaaggttca cgagaaaatt
     1861 accagaacgg taagaactat gcgcatgaag gcctgaaaaa atcatatagc actgatattc
     1921 atctttggca taacgggaaa tgggataaat cgggtgaatt atcgcaaggt cgggcctacg
     1981 gagtatcatt gccctggaat aatagtttat taattattgg cggtgaaact gcaggcggca
     2041 aagcggtgac ggattcagtt ttaatttctg tgaaggataa taaagtcaca gtacaaaact
     2101 aacgcttcag ggccccggta aaggggccct gctatcaatt tgtcatttgt attacgcgaa
     2161 aattatatga acgcaataat atcacccgat tattactatg ttcttaccgt tgctggtcag
     2221 tctaatgcca tggcgtatgg cgaaggactg ccattaccgg acagggaaga tgcgcctcat
     2281 tccagaatta aacaattagc gagatttgcg catacgcatc ccggaggccc gccatgtcac
     2341 tttaacgaca ttattccact gactcactgc ccacacgatg ttcaggatat gcagggttat
     2401 caccatcctc tggcaacgaa tcatcaaaca cagtacggca ccgttggcca ggcactgcat
     2461 attgcacgga aattactgcc cttcattcct gataatgcag ggattcttat cgttccgtgt
     2521 tgccgtggcg gatcggcttt taccgcgggc agcgaaggga catattcaga acggcacgga
     2581 gccagccatg atgcttgtcg ttggggaacg gatactccgc tgtaccagga tttagtcagc
     2641 agaacgcgag ccgcactggc aaaaaatccg cagaacaaat tcctcggcgt atgctggatg
     2701 caaggcgaat ttgacttaat gaccagtgac tacgcgtcac accctcaaca ctttaatcat
     2761 atggttgaag cctttcgtag ggatctaaaa caataccatt ctcagcttaa taatattact
     2821 gacgcgccgt ggttttgcgg cgataccacc tggtactgga aagaaaattt cccgcattcg
     2881 tatgaagcta tttatggcaa ttatcaaaat aatgttttag ccaatattat tttcgtcgac
     2941 ttccagcaac aaggtgaaag aggactgacg aacgcgcctg atgaagaccc ggacgattta
     3001 agcacgggat attacggttc agcgtaccgg tcaccggaga actggacgac ggcactgcga
     3061 agcagtcatt tcagcacggc agcccgtcgg gggattattt ctgacagatt tgtagaagca
     3121 attttgcagt tttggcgcga aaggtgagcg gggagtttat tcatggggga tgtccttgtt
     3181 cgttctcaga gtagacaaca aaaaacaaat gagatcaagg taatatctct tcacttgcgg
     3241 gctcgaacag caggcaattg ccaatataat aatctggcac aagggtgaaa aacgcgcacc
     3301 gcataaaccg ttgttattgc ttacagatta gccggatgcc ttaatagaca tccgcgcctt
     3361 ttcgattatg gctcggaaat ctacgaacat ctacacagtt tactggaacg ttttgggcca
     3421 cagcgttcgc agcaccgtcc tgatatgccg ttctggcgat tataagtcga cggattctgg
     3481 caaatagacg atgatgatat gcttaatgat gttcaaagaa attaggccat tattatatta
     3541 ttgcttgcat tttctttcat tcctaaaagc acccgccatt aatcccaggc gcattatg
//
LOCUS       DAZXUK010000320         3596 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZXUK010000320 DAZXUK010000000
VERSION     DAZXUK010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.320"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..892
                     /locus_tag="ACH3L8_003847"
                     /pseudo
     CDS             1..892
                     /locus_tag="ACH3L8_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(920..1009)
                     /locus_tag="ACH3L8_003848"
     tRNA            complement(920..1009)
                     /locus_tag="ACH3L8_003848"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(973..975),aa:Ser,seq:cga)
     gene            1103..1900
                     /gene="mtfA"
                     /locus_tag="ACH3L8_003849"
     CDS             1103..1900
                     /gene="mtfA"
                     /locus_tag="ACH3L8_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFT4086751.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2001..2076
                     /locus_tag="ACH3L8_003850"
     tRNA            2001..2076
                     /locus_tag="ACH3L8_003850"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2034..2036,aa:Asn,seq:gtt)
     gene            2238..3500
                     /locus_tag="ACH3L8_003851"
                     /pseudo
     CDS             2238..3500
                     /locus_tag="ACH3L8_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          988 a          839 c          862 g          907 t
ORIGIN      
        1 gtaaataatc gcgatcgtgc aatgacattc ggtcgcttgt gggaaaaatt cctcgccagc
       61 gcctattaca gtgacctcag tccaagaaca caaaaagatt atctgcaaca tcaaaaaaag
      121 ttgcttgccg tattcggtaa ggtgccggca gattccataa aaccagaaca catccgtcga
      181 tacatggaca agagagggga acagagtaaa acgcaagcca accatgaaaa aagcagtatg
      241 tcccgcgttt acagttgggg gtatgagcga gggtacgtga aggctaaccc atgtgcaggt
      301 gtaagtaaat tcaaggccaa aaaccgcgaa cgatatgtaa ccgacaaaga ataccaggca
      361 gtattaagcg ttgcacctct tcctgttttt atcgcaatgg aaattgccta tctgtgtgca
      421 gcgagggttt ccgatgtgtt atcgctgaaa tgggagcaga ttggaaacga cgggatcttt
      481 atccagcaag ggaaaacagg aaaaaaacag ataaaagcat ggagtccacg attacaggcg
      541 gcgatcgaaa aagcaaaaca gttaccaaca tccgcctatg taatcagcaa tcaatacggc
      601 aaccgatata tgtacaaagg ctttaacgaa atgtgggtag aagcaagaaa tcgcgcaggc
      661 aaaatttcag gtattttaac cgacttcacc tttcatgatc tgaaggcgaa aggaatttca
      721 gactatgaag gaagcagtcg ggataagcaa cttttctctg gtcacaaaac cgaggggcaa
      781 gtgctaatct atgacaggaa ggttaaagtt tcaccgacac ttgatgtccc gttacctgaa
      841 aatattccaa gaaaatattc caagtaattc caagtgtgat ttttgtcact gacttaatga
      901 tgtataagtg attgaatttt ggcggagaga gggggatttg aacccccggt ggagttgccc
      961 ccactccggt tttcgagacc ggtccgttca gccgctccgg catctctccg ttcagatggt
     1021 tgccatgatg ccaggaaatt tggcatttta acagtccctg tccgtgcaat tttgttcaag
     1081 tgacgagttt gcgagcaaaa cgatgattaa gtggccctgg aaagtacaag aatcagcaca
     1141 tcaaactgcc cttccctggc aggaagcact atcgatcccc cttttaacgt gtctgacaga
     1201 acaggaacaa agcaaattgg tcgctcttgc cgaacgtttt ttacagcaaa aacggcttgt
     1261 tcctttacag ggctttgagc tgaattcatt aagaagctgc cggatagcac ttctattttg
     1321 cctgcccgtt ctggagttag gactggaatg gctggatggt tttcatgaag tcttaattta
     1381 tcctgcgcca tttgtggtcg atgatgaatg ggaagacgat atcggtctgg tgcataacca
     1441 acgtattgtt cagtcaggtc agagctggca gcaagggcct atcgttttga actggttgga
     1501 tatacaagat tcttttgatg cttctggttt taacctgatt attcatgaag tcgctcataa
     1561 gctggacacc cgtaacggcg atcgcgccag cggagttccc tttatttcgt tgcgtgaggt
     1621 tgctggctgg gaacacgatc ttcatgctgc aatgaacaac attcaggaag aaatcgaatt
     1681 agttggtgag aatgcggcga gcattgatgc ttatgctgcc agtgatcctg ctgaatgttt
     1741 tgccgtactt tctgaatatt tctttagcgc cccagaactt tttgctcctc gtttcccttc
     1801 attgtggcaa cgtttctgtc aattttatca acaagatcct ttgcagagac tgcatcacgc
     1861 taatgataca gactcgtttt cagcgacgaa tgttcattaa ttaacaactt tgaagattaa
     1921 ttaaccaatt gaaatggctt atgaaattta gtgttgacag acaaggtacc gctaagtaat
     1981 atgcgccccg ttctcacgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc
     2041 cgtatgtcac tggttcgagt ccagtcagag gagccaattt tctgttttca tgcatccttg
     2101 cgaatcttta tctgatgttg attcaacagg ttagtgtgaa aactcttctc ggtgcatttt
     2161 gattttaccc tacgcatcgg gaaaaattgg tggtcaaatc tgggatcagg ttagttcgat
     2221 aatggagtga cccccatata tcccttaccg acgcaaaaat ccgcaccctc aagccttctg
     2281 ataaaccctt taaagtctcc gattctcacg gtctgtatct gctggtcaag ccgggtggct
     2341 cccgccactg gtatctcaaa taccgtatta gcggtaaaga atcccgcatt gcgctgggtg
     2401 cctatccagc catctccctg tctgatgcgc gacagcaacg tgaaggtatc cgtaaaatgc
     2461 tggcgctgaa tatcaacccg gtacagcagc gggctgctga acgtggctca cgaacaccgg
     2521 agaaagtttt taaaaacatg gcgctggcgt gacataaaag taacaggaaa tggtcgcaga
     2581 acaccgccga ccgtctgctt gccagcctga acaatcacat ctttccggtc atcgggaacc
     2641 tacctgtatc agaacttaaa ccccgtcatt tcattgacct gctgaaaggg atcgaggaaa
     2701 aaggtctgct ggaggttgcg tcccgcacac ggcagcacct gagtaacata atgcgccatg
     2761 cggtccatca ggagttaatc gatacgaacc ctgcagcaaa ccttggcggc gtgaccacac
     2821 ctcctgtcag acggcactat cctgccctgc cgctggagcg gctgcctgaa ctgcttgaac
     2881 gtattggggc atatcatcag ggccgtgaac tgacccggca tgccgttctg ctgatgctgc
     2941 atgtgttcat tcgctccagt gaactgcgtt tcgcccgctg gtcagagatt gatttcacaa
     3001 accgagtctg gacgataccc gcgacgcgag aacccattat tggcgtgcgt tattccggcc
     3061 gcggggcaaa aatgcgaatg ccgcatatcg tccccctctc agaacagtcc atcgccattc
     3121 tgaaacagat taaggatatc accggtaata atgaactgat cttccccggc gaccataacc
     3181 cgtataagcc aatgtgtgaa aacacggtca ataaggcact gcgggtgatg ggttacgaca
     3241 cgaaaaagga tatctgcggt cacggcttcc gggcaatggc atgcagtgcg ctgatggaat
     3301 cgggtttatg ggcaaaggac gcagtagaac gccagatgag tcatcaggag cgcaataccg
     3361 tgcgcatggc ttatattcat aaggcagagc acctggaagc ccgcaaagcg atgatgcagt
     3421 ggtggtcgga ttatctggaa gcatgccgag aatcttatgc accgccttat acaattggta
     3481 aaaataagtt tatcccatag tatactaacg aatcccacac tctggcaaat taccaccacc
     3541 tctacaggag gtggttctat atagactttt atcaacttca tgacgaaaaa caccta
//
LOCUS       DAZXUK010000321         3574 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZXUK010000321 DAZXUK010000000
VERSION     DAZXUK010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.321"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            44..1195
                     /gene="yiaY"
                     /locus_tag="ACH3L8_003852"
     CDS             44..1195
                     /gene="yiaY"
                     /locus_tag="ACH3L8_003852"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFT4086752.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            1360..2898
                     /gene="aldB"
                     /locus_tag="ACH3L8_003853"
     CDS             1360..2898
                     /gene="aldB"
                     /locus_tag="ACH3L8_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFT4086753.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3117..3260)
                     /locus_tag="ACH3L8_003854"
     CDS             complement(3117..3260)
                     /locus_tag="ACH3L8_003854"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086754.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3443..>3574
                     /locus_tag="ACH3L8_003855"
     CDS             3443..>3574
                     /locus_tag="ACH3L8_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFT4086755.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
BASE COUNT          888 a          856 c          939 g          891 t
ORIGIN      
        1 tcagtaagct gcccaccctt ttttacactt tcaggagtgt gttatggcat cttcaacttt
       61 ctttattcct tctgtgaatg tcatcggcgc tgattcattg actgatgcaa tgaatatgat
      121 ggcagattat ggatttaccc gtaccttaat tgtcactgac aatatgttaa cgaaattagg
      181 tatggcgggt gatgtgcaaa aagcactgga agaacgcaat atttttagcg ttatttatga
      241 tggcacccaa cctaacccca ccacggaaaa cgtcgccgca ggtttgaaat tacttaaaga
      301 aaataattgc gatagcgtga tctccttagg cggtggttct ccacacgact gcgcaaaagg
      361 tattgcgctg gtggcagcca atggcggcga tattcgcgat tacgaaggcg ttgaccgctc
      421 tgcaaaaccg cagctgccga tgatcgccat caataccacg gcgggtacgg cctctgaaat
      481 gacccgtttc tgcatcatca ctgacgaagc gcgtcataac aaaatggcga ttgttgataa
      541 gcacgtgact ccgctgcttt ctgtcaatga ctcctctctg atgattggta tgccgaagtc
      601 actgaccgcc gcaacgggta tggatgcctt aacgcacgct atcgaagcgt atgtttctat
      661 tgccgccacg ccgatcactg acgcttgtgc actgaaagcc gtgaccatga ttgccgaaaa
      721 cctgccgtta gccgttgaag atggcagtaa tgcgaaagcg cgtgaagcaa tggcttatgc
      781 ccagttcctt gccggtatgg cgttcaataa tgcttctctg ggttatgttc atgcgatggc
      841 gcaccagctg ggcggtttct acaacctgcc acacggtgta tgtaacgccg ttctgctgcc
      901 gcatgttcag gtattcaaca gcaaagtcgc cgctgcacgt ctgcgtgact gtgccgctgc
      961 aatgggcgtg aacgtgacag gtaaaaacga cgcggaaggt gctgaagcct gcattaacgc
     1021 catccgtgaa ctggcgaaga aagtggatat cccggcaggc ctacgcgacc tgaacgtgaa
     1081 agaagaagat ttcgcggttc tggcgactaa tgccctgaaa gatgcctgtg gctttactaa
     1141 cccgatccag gcaactcacg aagaaattgt ggtgatttat cgcgccgcaa tgtaatcatc
     1201 attccaacaa cggctggcaa attgtcagcc gctttttcaa ctatctctgt aatccttgcc
     1261 cgtaaattcg tgatagctgt cgtaaagctg ttaccgactg gcgaagattt cgccagtcac
     1321 gtctaccctt gttatacctc acaccgcaag gagacgatca tgaccaataa tcccccttca
     1381 gcacagatta agcccggcga gtatggtttc cccatcaagt taaaagcccg ctacgacaac
     1441 tttattggcg gtgaatgggt agcccctgcc gatggtgagt attaccagaa tctgacgccg
     1501 gtgaccgggc agctgctgtg cgaagtggcg tcttcgggca aacgagacat cgatctggcg
     1561 ctggatgctg cgcacaaagt gaaagataaa tgggcgcaca cctcggtgca ggatcgcgcg
     1621 gcgattctgt ttaagattgc cgatcgaatg gaacaaaacc tcgagctgtt agcgacagct
     1681 gaaacctggg ataacggcaa acccattcgc gaaaccagtg ctgcggatgt accgctggcg
     1741 attgaccatt tccgctattt cgcctcgtgt attcgggcgc aggaaggtgg gatcagtgaa
     1801 gttgatagcg aaaccgtggc ctatcatttc catgaaccgt taggcgtggt ggggcagatt
     1861 atcccgtgga acttcccgct gctgatggcg agctggaaaa tggctcccgc gctggcggcg
     1921 ggcaactgtg tggtgctgaa acccgcacgt cttaccccgc tttctgtact gctgctaatg
     1981 gaaattgtcg gtgatttact gccgccgggc gtggtgaacg tggtcaatgg cgcaggtggg
     2041 gaaattggcg aatatctggc gacctcgaaa cgcatcgcca aagtggcgtt taccggctca
     2101 acggaagtgg gccaacaaat tatgcaatac gcaacgcaaa acattattcc ggtgacgctg
     2161 gagttgggcg gtaagtcgcc aaatatcttc tttgctgatg tgatggatga agaagatgcc
     2221 tttttcgata aagcgctgga aggctttgca ctgtttgcct ttaaccaggg cgaagtttgc
     2281 acctgtccga gtcgtgcctt agtgcaggag tcaatctacg aacgctttat ggaacgcgcc
     2341 atccgccgtg tcgaaagcat tcgcagcggt aacccgctcg acagcgtgac gcaaatgggc
     2401 gcgcaggttt ctcacgggca actggaaacc atcctcaact acattgatat cggtaaaaaa
     2461 gagggcgctg acgtgctcac aggcgggcgg cgcaagctgc tggaaggtga actgaaagac
     2521 ggctactacc tcgaaccgac gattctgttt ggtcagaaca atatgcgggt gttccaggag
     2581 gagatttttg gcccggtgct ggcggtgacc accttcaaaa cgatggaaga agcgctggag
     2641 ctggcgaacg atacgcaata tggcctgggc gcgggcgtct ggagccgcaa cggtaatctg
     2701 gcctataaga tggggcgcgg catacaggct gggcgcgtgt ggaccaactg ttatcacgct
     2761 tacccggcac atgctgcgtt tggtggctac aaacaatcag gtatcggtcg cgaaacccac
     2821 aagatgatgc tggagcatta ccagcaaacc aagtgcctgc tggtgagcta ctcggataaa
     2881 ccgttggggc tgttctgata taagaagctg gtcgcattgg gtattcattg cctgatgcga
     2941 cgcttacgcg tcttatcatg cctacgggaa cctgaccgta ggccggataa ggcgtacacg
     3001 ccgcatccgg cataaacaaa gcgcacgttg ttacaattta cgttttaaac ctacgtgcgc
     3061 aataacccag actacgccaa cagaagtgcg tttctggggc gtgttttgtc aggagtttaa
     3121 tctcgcgttt cgatggtgag cgggcgaacc aggccgtaaa tcaccattgg tgatccgagg
     3181 ccaatcagca ataaacggta gataattgaa ggtttaatac gcttaatctg tactgtggat
     3241 gttttactgg agttattcat tagtttggcc aatagttcca ctatttcaat atgataaacc
     3301 tcgtcctgtt gaaaaaccag acagcacatt tcagaaagta tataaaaaac aatatgtcgt
     3361 tatataaata aggaaattgc actgcatcat ggcatttcat aatatgcgtt aattgttctt
     3421 ttttatattc tttgagggaa atatgttcct ggactatttt gcactgggag tgcttatttt
     3481 tgtatttctg gtgatattct atgggattat tattttacat gatattccct acctgattgc
     3541 taaaaaacgt aatcatcctc atgccgacgc catt
//
LOCUS       DAZXUK010000322         3562 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZXUK010000322 DAZXUK010000000
VERSION     DAZXUK010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.322"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..220
                     /locus_tag="ACH3L8_003856"
     CDS             <1..220
                     /locus_tag="ACH3L8_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4086756.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(392..721)
                     /locus_tag="ACH3L8_003857"
     CDS             complement(392..721)
                     /locus_tag="ACH3L8_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFT4086757.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(726..911)
                     /locus_tag="ACH3L8_003858"
     CDS             complement(726..911)
                     /locus_tag="ACH3L8_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFT4086758.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(908..3547)
                     /locus_tag="ACH3L8_003859"
     CDS             complement(908..3547)
                     /locus_tag="ACH3L8_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFT4086759.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWLYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
BASE COUNT          871 a          961 c          810 g          920 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat
      301 ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct
      361 gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta
      421 tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg
      481 cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt
      541 cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat
      601 aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca
      661 ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca
      721 tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg
      781 atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca
      841 gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt
      901 aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac
      961 gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg
     1021 taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc
     1081 gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt
     1141 ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca
     1201 tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc
     1261 tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg
     1321 caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc
     1381 tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt
     1441 tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt
     1501 cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat
     1561 taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat
     1621 cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg
     1681 cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag
     1741 aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca
     1801 ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa
     1861 taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt
     1921 taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta
     1981 acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg
     2041 agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt
     2101 ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa
     2161 tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa
     2221 aactcccttt tccccagtag agccactgcc agcggccagc gtcagggaga aaatcattcg
     2281 ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg
     2341 cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga
     2401 tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct
     2461 caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata
     2521 aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca
     2581 tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg
     2641 tcagcacatt cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga
     2701 ctttgacacc cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat
     2761 cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt
     2821 tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca
     2881 ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc
     2941 catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa
     3001 acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt
     3061 taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt
     3121 ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag
     3181 gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg
     3241 gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc
     3301 tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac
     3361 ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac
     3421 gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca
     3481 gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac
     3541 ccagcatggg tagttaatat cc
//
LOCUS       DAZXUK010000323         3530 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZXUK010000323 DAZXUK010000000
VERSION     DAZXUK010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.323"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            56..1309
                     /locus_tag="ACH3L8_003860"
                     /pseudo
     CDS             56..1309
                     /locus_tag="ACH3L8_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1358..1477)
                     /locus_tag="ACH3L8_003861"
     CDS             complement(1358..1477)
                     /locus_tag="ACH3L8_003861"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFT4086760.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            1733..2041
                     /gene="asr"
                     /locus_tag="ACH3L8_003862"
     CDS             1733..2041
                     /gene="asr"
                     /locus_tag="ACH3L8_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFT4086761.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQPAKPAAQPAA
                     "
     gene            2134..2217
                     /locus_tag="ACH3L8_003863"
     CDS             2134..2217
                     /locus_tag="ACH3L8_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086762.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            2317..3138
                     /locus_tag="ACH3L8_003864"
     CDS             2317..3138
                     /locus_tag="ACH3L8_003864"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFT4086763.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(3177..3506)
                     /gene="mdtI"
                     /locus_tag="ACH3L8_003865"
     CDS             complement(3177..3506)
                     /gene="mdtI"
                     /locus_tag="ACH3L8_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFT4086764.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
BASE COUNT          837 a          905 c          869 g          919 t
ORIGIN      
        1 ttttcctacc ctatgtataa gcctgatcca caggcatatt tagcaaggat ttcaagtgag
       61 ccgtactaca actgttgatg gcgctccggc aagcgacact gacaagcaaa gcatttctca
      121 gccaaatcaa tttattaaac gcggtacgcc gcaatttatg cgcgtcaccc tggcgctgtt
      181 ctctgccgga ctggcaacct ttgcacttct ctattgtgtg cagcctatcc ttccggtgct
      241 ttcgcaggag tttggcttaa cgcccgcgaa cagtagtatt tcactgtcca tttccacggc
      301 gatgttggct atcggtttgc tatttactgg cccgctatcc gatgccattg gtcgcaaacc
      361 agtgatggtc acggcgctac tgttggcctc catttgtacg ttactttcga caatgatgac
      421 cagctggcac ggcattttga ttatgcgcgc cttgattggg ctttcgttaa gtggcgtggc
      481 agctgttggc atgacttatc ttagcgagga aatccacccc agtttcgtgg ccttttcgat
      541 ggggttgtat atcagcggca actcaattgg cggcatgagc ggacgcttaa ttagtggtgt
      601 cttcacggac tttttcaact ggcgaattgc tctggcggca atcggttgtt tcgcgctggc
      661 ctcggcgttg atgttctgga aaatcctccc tgaatcacgc catttttgcc cgacttcgct
      721 gcgccctaag acgttattta tcaactttcg tctgcactgg cgtgaccggg gattaccgtt
      781 attgttcgca gaaggctttt tgctgatggg gtcgttcgtc acgctgttta attacatcgg
      841 ctatcggttg atgctttccc cctagcatgt cagtcaggcc gtggttggct tattatcgct
      901 ggcttatttg accggtacat ggagctcacc caaagccgga accatgacca cccgctatgg
      961 gcgcggtccg gtgatgttgt tttcgacggg agttatgccg tttggtttac tgatgacctt
     1021 atttagctcg ctgtggctga tctttgccgg aatgttactc ttctcagccg gattcttcgc
     1081 agcccactca gtagccagca gctggatcgg cccccgcgca aaacgcgcta aaggccaggc
     1141 ctcctcgctg tatctgttca gttactatct ggggtcgagt attgccggga cgctgggtgg
     1201 tgttttctgg cataactatg gctggaacgg cgtcggcgca tttattgctc tgatgctggt
     1261 cattgctctg ctggtcggga cgcgtttgca tcgtcgtctg cacgcctgaa aaataagtcc
     1321 ggactgcggt aaatacccgt ccggacttat tgcctgctca aaccaacgtt aatagccatc
     1381 ctaaaataga cgaagcgcca gccaattccc gcagcgcatc tagcgtcatc aggattataa
     1441 gtacccaaat aaacggattc attttgctgt gtgtcattta ttactgatgc gcagttattc
     1501 taccgctttg taagtagtat aatagttaac ccgatcaaga ctactattat tggtagctaa
     1561 attttcctta agtcacaata cgttattatc aacgctgtaa tttattcagc gtttgtacat
     1621 atcgttacac gctgaaacca accactcacg gaagtctgcc attcccaggg atatagttat
     1681 ttcaacggcc ccgcagtggg gttaaatgaa aaaacaaatt gagggtatga caatgaaaaa
     1741 agtattagct ctggttgttg ccgctgctat gggtctgtct tctgccgcct ttgctgcaga
     1801 gactgcgacc acacctgctc cgactgcgac gaccaccaaa gcagcgccag cgaaaactac
     1861 acatcataaa aaacagcata aagcagcacc tgcccagaaa gcgcaggcgg ctaaaaagca
     1921 tcataaaaat acgaaagctg aacagaaagc ccctgaacaa aaagcgcagg cagcgaagaa
     1981 acacgccggg aaacacggcc atcagcaacc ggcaaaacct gctgcacaac ccgcagcgta
     2041 agttttcaac agtaatgctg gcgcgccccc tcgcgcctga aaattacggt gctaagcggg
     2101 taacgtttag caccgccttt agcggagggt agtatggtgg gccgttatcg ctttgagttc
     2161 attcttatca tccttatttt atgcgcactg attgccgccc gtttttatct ttcctgactg
     2221 tagttatctg attttactcc cactttcatc ccgtcccgtc tatagtattt atgagggttt
     2281 gcttttaata atcataatta cccaccagag tgtgatatgc gtacaaccat tgctgtagtg
     2341 ttgggtgcaa ttagtttgac gtctgctttt gtgtttgcag ataaaccaga cgttgccaaa
     2401 tcggcaaacg atgaggtcag caccctgttt tttggtcatg atgatcgtgt gccagtgaat
     2461 gacacgaccc aatcaccgtg ggatgcggtt gggcaactgg aaacggccag cggcaattta
     2521 tgtacggcga cgctgattgc acccaatctg gcattaacgg caggacactg tttattgacg
     2581 ccgcctaagg gtaaagcgga taaggcggta gcgctgcgtt ttgtgtcaaa taaaggtctt
     2641 tggcgctatg agatccacga catagaaggc cgcgttgatc cgacactggg aaagcggtta
     2701 aaagcagatg gggatggttg gattgttcct cccgcagctg caccgtggga cttcggattg
     2761 attgtgctac gtaatccccc ttctggcatt acaccgttgc cgttatttga gggagataaa
     2821 gccgcgctta ctgccgcatt aaaatcggct ggtcgtaaag tgactcaggc gggataccct
     2881 gaagatcatc tcgatacgtt atacagtcat caaaactgtg aagtgactgg ctgggcgcaa
     2941 acgtcggtga tgtcacatca gtgcgatacc ttgccgggtg acagcggttc gcctctgatg
     3001 ttgcataccg atgacggctg gcaattaatt ggggtgcaaa gttcggctcc tgccgcgaaa
     3061 gatcgctggc gcgccgataa ccgggccatt tctgttaccg gttttcgcga caagctggat
     3121 caactgtcgc aaaaataatg ttcaaacgct gcccgacagc gcgggcagcg tcttcatcag
     3181 gcaagtttca ccatgatcat tccagccaac agcaagacca ggccaatcca gcctttacga
     3241 tttaaccgtt gaccaaacaa gatccaacct gcggctaacg tggcggcaat accaaacccg
     3301 ccccacaatg cataaacgac agacaagtcg atccctttaa cggcctgaga aagcgcacta
     3361 aaggccgcca gcaccgccgc cagggagagc aagccaaata ttttgcgacg aaagccgtca
     3421 gaaaatttca aaaagacgtt agcaacgatt tccagcacga ttgccaatgc cagccaggcg
     3481 gcgtgaaccc attcaaactg cgccatggtt cacctccagt tcaggtttac
//
LOCUS       DAZXUK010000324         3456 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZXUK010000324 DAZXUK010000000
VERSION     DAZXUK010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.325"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..739)
                     /locus_tag="ACH3L8_003866"
     CDS             complement(<1..739)
                     /locus_tag="ACH3L8_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086765.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNDSQELNNSQKLNN
                     SQELNNSQVSCKGSVDSTITDLLEKALN"
     gene            complement(862..1314)
                     /gene="tabA"
                     /locus_tag="ACH3L8_003867"
     CDS             complement(862..1314)
                     /gene="tabA"
                     /locus_tag="ACH3L8_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFT4086766.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(1459..2052)
                     /locus_tag="ACH3L8_003868"
     CDS             complement(1459..2052)
                     /locus_tag="ACH3L8_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFT4086767.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            2123..2836
                     /gene="bdcA"
                     /locus_tag="ACH3L8_003869"
     CDS             2123..2836
                     /gene="bdcA"
                     /locus_tag="ACH3L8_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFT4086768.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2968..3363
                     /locus_tag="ACH3L8_003870"
     CDS             2968..3363
                     /locus_tag="ACH3L8_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFT4086769.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
BASE COUNT          910 a          827 c          748 g          971 t
ORIGIN      
        1 tattcaatgc tttttctaat aaatccgtaa tcgtagaatc aactgaacct ttacaactca
       61 cctgcgagtt atttaactcc tgcgaattat ttaacttctg tgaattattt aactcctgcg
      121 aatcatttaa ctcctgcgaa tcatttaact cctgtatatt atttaacttc tgatgattat
      181 ttaatagacg cgctattttc ttgttagctt cgcgttggta acacgccaga accttgttag
      241 taatcaggtg taatgaacta tcatcttttc tttcaaaaga cttcccttca taacgattag
      301 tcgaaccatc agattgttta aatataatga ctgtatgtcc ttcacgaatc cctttagtag
      361 aagctgaggc aagtgtaatt tctatcttca ccttagataa atccgctcgc tccccttcag
      421 gattattcat gtaaaacttc attttttcat tgccatcagt atctttagaa taagatgaga
      481 actcaatatg agccatcata tttctacccg cttcgttgga gcgtttgcta aaatccacat
      541 aacccagggt aaatatcttt gccaaaaact ccaaaaatgc tctaggagaa agaatattgc
      601 ttctgctatt ctgccccccg gcaattcggg caaaatcatt cattcccaca taccaggtat
      661 ctaaggcttc atttttttgt ttaaaattgt cggctaatgt aatgtgttga gaataattca
      721 aatctgatat tttgcccatg acgaactcct tgagttaata agaacgtcta agcattacaa
      781 attttactca caaaaaaata tcaaaaaacg ctcttttcac aaactcgccg taaacctgga
      841 gttatttacg gcgaaaagtc atcacgccat cagcatcttc actactgctt tgcgaacctg
      901 ggctggcgcg cccactgcgc acagcggttt atgcacttcc cccggataaa acacaacaaa
      961 atcaccttca tttaggataa ctgttttctc atcaacgcct tccggcaaaa atgcgatgtc
     1021 tttatcagct aaccagtcgg tatccggcgc gcctgcaggt tgcgtgctga aggtcatgcc
     1081 ttcctgacct tttaacacaa tctgaatgtc aagatagcgg gcatggtact ccgcacggcg
     1141 agcttcgtac ggctcggtca tatcttccga gataagataa aacagacgat tgccttcgat
     1201 atcatgcttg ccctttggcg tttctgccgt aacgtgtgct ttgatatgct caatcgcctg
     1261 gcgtaactcc tggggtagcc acggctgaag attatgaata tttccgatga tcatcttttc
     1321 cactccttaa ttaaaacaat gtttcatttc tatcgttata cgaaaagatg atcaattccg
     1381 ccagccttaa cgcgtgaatt attgtcttat cacaacttat cggcaggcgc gttcccgcct
     1441 ttcggatcaa ggccagcatc actccttgag aagagttttt atcgcttccc ctgccagggc
     1501 agctgttgca cagagttgtt cgatggagtg cccctcccgt gccttcgcag aaagccctga
     1561 catcacggta ctcataaaat cagtcacaca ttgtgcgctt tgtggatgcc gcctggcgat
     1621 atagtcataa atggtcgttt ctgcggcgtg ataatattga atggcaatat cacgcgcttg
     1681 tggatcatga ctatgaatac cttcaagaac catacagcca gcgcagccgc cgttttggct
     1741 atatctgcgc gccgcttctt ttaatacctc aaccaggcac tcgcctaccg gacgatcatc
     1801 acgaagaata tcggcaagcg gaatagcttc cgtaccgaca tattcattga gtacacggct
     1861 aaataaccca gccttactgc caaaagccgc gtagagactc ggggggttaa taccaagata
     1921 atcagtaact tcagagacac ttactgcatc gaaacctttt tggtgaaaaa gcacttttgc
     1981 cgcagagact gcctgctcag gagcgaaacg tctgggacga cctggaacgc gagattgttt
     2041 tttagtgacc attacaaaac ctgttgacag aaagttaaaa cagttttgta atgcatgtta
     2101 cataataaat caaggagtcc ttatgggcgc ttttaccggt aagacagttc tcatcctcgg
     2161 tggcagtcgt ggaatcggtg ccgctatcgt acgtcgtttc gtcaccgatg gggccaatgt
     2221 acgattcacc tatgcggggt cgaaagatgc cgctgaacgt ctggcacaag agactggagc
     2281 gacagcagta ttcacagata gtgctgacag agacgctgtt attgatgtcg ttcgtaagag
     2341 cggcgcattg gatatcctgg tggtaaatgc aggtattggc gtctttggcg atgccctgga
     2401 attaaatgcc gacgatattg atcgcctttt caaaatcaat attcatgctc cttatcatgc
     2461 ctctgttgaa gccgcccggc agatgcccga aggcgggcgc atcttaatca tcggctccgt
     2521 gaatggcgat cgtatgcctg ttgcaggcat ggctgcttat gccgccagta aatctgccct
     2581 gcaaggcatg gcgcgcgggc tggcccgtga ttttggaccg cgagggatca ccatcaacgt
     2641 cgtccagcca ggaccaattg ataccgacgt taatcccgcc aacgggccaa tgcgcgatat
     2701 gttgcatagt tttatggcca tcaaaagaca tgggcaaccg gaagaggtcg ctggtatggt
     2761 cgcatggtta gcagggccag aagccagttt tgttaccggc gcgatgcata ccattgatgg
     2821 cgcgtttggc gcataaccga ctacgctcaa ttaagcccag ccattatcca tgatgtctgg
     2881 gttttgttta ctaacgtcgt ccccctaaaa gcggctcccg ataaatataa atcttctgtt
     2941 catgtcgtaa atcttgagga taaccaaatg gtagaaagaa ccgctgtttt ccctgctggc
     3001 cgacattcac tgtatgctga gcatcgttat tctgcggcta ttcgttccgg cgatttgctg
     3061 tttgtttccg ggcaagtagg aagtcgagag gacggaacac cagaacccga ttttcagcaa
     3121 caagtcagac tggcctttga taatttgcat gcgaccctgg cagctgcggg atgcactttt
     3181 gacgatatca ttgatgttac gagctttcat accgatccgg aaaaacaatc tgaagacatc
     3241 atgacggtga aaaatgaaat atttagcgcc ccaccttatc caaactggac ggcggtgggt
     3301 gttacctggc tggcaggctt tgattttgaa attaaagtga tagcgcgtat ccctgagcta
     3361 taagcaatag tgttagccgt tcgttttcac cctccgccct ataagtcgga tgaatggaat
     3421 aaaatgcatt tctgattgcg tgaaaatgaa aaagaa
//
LOCUS       DAZXUK010000325         3448 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZXUK010000325 DAZXUK010000000
VERSION     DAZXUK010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.326"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..66
                     /locus_tag="ACH3L8_003871"
     CDS             <1..66
                     /locus_tag="ACH3L8_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFT4086770.1"
                     /translation="AELITGKLYDRIPKEIWKYVR"
     gene            105..695
                     /locus_tag="ACH3L8_003872"
     CDS             105..695
                     /locus_tag="ACH3L8_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFT4086771.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            935..1195
                     /locus_tag="ACH3L8_003873"
     CDS             935..1195
                     /locus_tag="ACH3L8_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFT4086772.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1419..1493
                     /gene="tap"
                     /locus_tag="ACH3L8_003874"
     CDS             1419..1493
                     /gene="tap"
                     /locus_tag="ACH3L8_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFT4086773.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1486..2343
                     /gene="repA"
                     /locus_tag="ACH3L8_003875"
     CDS             1486..2343
                     /gene="repA"
                     /locus_tag="ACH3L8_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFT4086774.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3044..3235
                     /locus_tag="ACH3L8_003876"
     CDS             3044..3235
                     /locus_tag="ACH3L8_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086775.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          927 a          849 c          865 g          807 t
ORIGIN      
        1 gcggaattaa tcaccggaaa gttatatgac aggataccaa aagagatatg gaaatatgta
       61 aggtaataat tcagcaataa ttaattgcca ggagcagata aataatgaag ttgattattt
      121 ttattctcat tgttctgatt attgcagcgc tacttatccg gattatcctc agaagtgtaa
      181 atcagcattc tcctttgctg atgcaattgc atgcggcagg tatacggact ggtgatgcag
      241 agcgaatact gtccagcggt gaatactggc aacgtcagaa gacgctgctg acagaaaggg
      301 aagtcagttt tatgaaagga ctgttcagaa ttgtggatat gaagcggtgg tatctgtgtc
      361 cgcaggtacg ggtagcggat atcgtccagc tgaacgggaa tatccggcca cgatcacgcc
      421 agtggtggca gttattcagg atggtgtctc agtggcatgt tgatgtggtt atcgttgagc
      481 tgcgttcgtt cagtattgtt gcagcagtag agctggatga tgccagccat ttacgaccgg
      541 aacgcagacg ccgggatatt cttctggaag aggttctgag gcaggctggt attccgttgc
      601 tcagaagcca cgatgccaga aaactgctgc agatgaccgg agaatggctg aatacaacag
      661 gggctgatca gcagtcaccg gaacatcgta gctgacgcct tcgcgttgct cagttgtcca
      721 accccggaaa cgggaaaaag caagttttcc tcgctcccgg cgtttccata actgaaaacc
      781 atactatttc acagtttaaa tcacattaaa cgacagtaat ccccgttgat ttgtgcgcca
      841 acacagatct tcgccacaat tctcaagtcg ctgatttcaa aaaactgtag tatcctctgc
      901 gaaacgatcc ctgtttgagt attgaggagg cgagatgtcg cagacagaaa atgcagtgac
      961 ttcctcattg agtcaaaagc ggtttgtgcg cagaggtaag cctatgactg actctgagaa
     1021 acaaatggcc gctgttgcaa gaaaacgtct tacacacaaa gagataaaag tttttgtcaa
     1081 aaatcctctg aaagatctca tggttgagta ctgcgagaga gaggggataa cacaggctca
     1141 gttcgttgag aaaatcatca aagatgaact gcagagactg gatatactaa agtaaagact
     1201 ttactttgtg gcgtagcatg ctagattact gatcgtttaa ggaattttat ggctggccac
     1261 gccataaggt ggcagggaac tggttctgat gtggatgtac aggagccaga aaagcaaaaa
     1321 ccccgataat cttctttaac tttggcgagt cagaaagatt accggggccc acttaaaccg
     1381 tatagccaac aatcaagcta tgcggggagt atagttatat gcccggaaaa gttcaagact
     1441 tctttctgtg ctcgctcctt ctgcgcattg taagtgcagg atggtgtgac tgatcttcaa
     1501 caaacttatt accgccaggt aaagaacccg aatccggtgt tcacaccccg tgaaggtgcc
     1561 cggacgcttc cgttctgcgg gaaactgatg gaaaaggcgg tgggcttcac ctcccgtttt
     1621 gatttcgcca ttcatgtggc acatgcccgt tcgcttggtc tgcgtcggcg catgccaccg
     1681 gtactacgtc gccgggccat tgatgcgctg ctgcaggggc tgtgttttca ctatgaccca
     1741 ctggccaacc gcgtccagtg ttccatcacc acgctggcca ttgagtgcgg actggcgacg
     1801 gagtctgctg ccggaaaact ctccatcacc cgggccaccc gggccctgac gttccttgca
     1861 gagctgggac tgattaccta ccagacggaa tatgacccgc ttatcgggtg ctacattccg
     1921 accgatatca cgttcacatc tgcactgttt gctgccctcg atgtatcaga ggaggcagtg
     1981 gccgccgcgc gccgcagtcg tgtggaatgg gaaaacagac agcgcaaaaa gcaggggctg
     2041 gataccctgg gtatggatga actgatggcg aaagcctggc gttttgttcg tgagcgtttc
     2101 cgcagttacc agacagagct taagtcccgt ggaatgaagc gtgcccgtgc gcgtcgtgat
     2161 gcggacaggc agcgtcagga tattgtcacc ctggtgaaac ggcaactgac gcgtgaaatc
     2221 tcggaagggc gcttcactgc cagtcgtgag gcggtaaaac gtgaagtgga gcgtcgtgtg
     2281 aaggagcgca tgattctgtc acgtaaccgt aattacagcc ggctggccac agcttccccc
     2341 tgaaagtgac ctcctcagaa taatccggca cgcgccggag gcattcgcac gcctgaagcc
     2401 cgccagtgca caaaaaatca gcgctacata caaaaaacaa cctcatcatc caccttcagg
     2461 tgcatccggt cccccctgtt tttgatacaa aacacgcctc acagacgggg aattttgctt
     2521 atccacatta aactgcaagg gacttcacca taaggttaca accgttcacg tcataaagcg
     2581 ccagacgtca accctacagg gtgcaatgta tcttttaaac acctgtttat atctccttta
     2641 aactacttaa ttacattcat ttaaaaagaa aacctgttca ctgcctgtcc tgtggacaga
     2701 caaatatgca cctcccaccg caagcggcgg gcccccaacc gaagccggtt tagttacaac
     2761 atgcaaacat aaacccctaa aaaacccaag tccaacgcaa aactgaaacc acaacgcccc
     2821 cctcataact gaaaagcgcc gccgcccccg cccaaagggc gggaacagag tcgcttttaa
     2881 ttatgaatgt tgtaactaca tcatcatcgc tgtcagtctt ctggctggac gtatcgaata
     2941 cacgctcgta agcggccctc acggcccgct aacgcggaga tacgccccga cttcgggtaa
     3001 aaccctcgtc gggaccactc cgaccgcgca cagaagctct atcatggctg aaaacgggta
     3061 tggcttggca gggctgggga tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc
     3121 gggcttcggc ggttttactc cggtatcaca tgtaaccaca gcgtgccgcc ttccatgccg
     3181 ctggcgcggc atcagaataa tacaagcagc atttaatgca tttgcaaaag tgtgagtaat
     3241 attaagtggt cgtttcaggg atatcgttaa gacttgagcg ataggaatta aaaccccaaa
     3301 aagattaaaa aaacaccaca aaacggatgt ttcttcaaca ccacttttgc tccatatgaa
     3361 cggaaccgac gattaaactg gatggctctg attgattcag ggtatgaatg gcggtttttt
     3421 gctccgtttc cctcaaaatg gacgcaac
//
LOCUS       DAZXUK010000326         3447 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZXUK010000326 DAZXUK010000000
VERSION     DAZXUK010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.327"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..692
                     /locus_tag="ACH3L8_003877"
     CDS             <1..692
                     /locus_tag="ACH3L8_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFT4086776.1"
                     /translation="NVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDDAV
                     RSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWLVVNQIG
                     GKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVDSWKVIVVEPKKQLTL
                     LFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMAK
                     QIARLAEQSTD"
     gene            791..1804
                     /locus_tag="ACH3L8_003878"
     CDS             791..1804
                     /locus_tag="ACH3L8_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFT4086777.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1801..2631)
                     /gene="amiD"
                     /locus_tag="ACH3L8_003879"
     CDS             complement(1801..2631)
                     /gene="amiD"
                     /locus_tag="ACH3L8_003879"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFT4086778.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2628..2951)
                     /locus_tag="ACH3L8_003880"
     CDS             complement(2628..2951)
                     /locus_tag="ACH3L8_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFT4086779.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(3162..>3447)
                     /locus_tag="ACH3L8_003881"
     CDS             complement(3162..>3447)
                     /locus_tag="ACH3L8_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFT4086780.1"
                     /translation="AGNNVKVAADAFLEIIFSLKNKYCIKLLSAQDRAFIYEFARMIF
                     AYINDKSIEILLLSCFAAADQKAIQRYRPQSQDGEDFRSHLQYKLPLSAH"
BASE COUNT          820 a          924 c          862 g          841 t
ORIGIN      
        1 tcaatgtgat tacttccgta ccgcccacca ccgccagggc gttgattcag gggctgaaac
       61 acgatctgct ggcagatgat accgcgctac gtgcactcat cccacaacgg ctgatcgctt
      121 tcgatgacgc ggtacgtagc acgttgaaag aggaagaaaa actggtcaac tccagcgact
      181 ggggctacga cgctcaggcc tttgcccgct ggcgaccgga gtacggttat ttcgccaaac
      241 aggcggggtt taccgttaaa acgtccgcca gccttgcggc tttatggctg gtggtgaacc
      301 aaatcggcgg taaagagcgt tatttctttg gcaatatttt gtggcagaca cgggcgttga
      361 tggaccgcgc gatcggtcat aatctggcga aaggccgccc ggagcgcgaa tatttacaga
      421 ccggcgatgc ggtggatagc tggaaagtga ttgtcgttga accgaaaaag caacttacgt
      481 tgttatttgg catgaaagcg ccggggctgg gacgactgtg ttttaccctg gaagataaag
      541 gtgactatcg tactatcgat gtccgcgctt tctggcatcc gcacggtatg ccggggctgt
      601 tttactggtt attgatgatc cccgcgcatc tgtttatttt tcgcggaatg gcaaaacaaa
      661 tcgccagact ggcagaacaa agcacagatt aatcgacaaa acctgcgtta ttctttcatg
      721 tttcccattg catcatgggg gaaatcacgg aagaatgcgc acgtcatttt ctcaacaaca
      781 gtggattgat atgaaggtac tggttaccgg cgccaccagc ggcttaggtc gaaacgcggt
      841 agagttttta tgccagaaag gcatcagcgt gcgtgcgagc ggtcgcaacg aagcaatggg
      901 taaattgctg gaaaaaatgg gcgcagagtt tgttccgacg gatctgaccg agctggtttc
      961 atcacaagct aaagtgatgc tcgcgggcat tgatacgctg tggcactgct ccagctttac
     1021 ctcaccctgg gggacacaac aggctttcga tctggctaac gttcgcgcca ctcgccgcct
     1081 gggtgaatgg gctgtcgcct ggggtgtgcg taactttatt catatctctt ccccctccct
     1141 gtacttcgat tatcaccacc atcgcaatat taaagaagat tttcgccctc accgcttcgc
     1201 caacgagttt gcccgcagca aagcagccag cgaagaagtg atcaatatgc tttcgcaggc
     1261 gaatccacaa acgcgcttta ctattctgcg cccacaaagt ctgttcggac cgcacgataa
     1321 agtctttatt ccccgtctgg cgcatatgat gcaccactac ggcagtattc tgttaccgca
     1381 tggcggcagt gcgctggtcg atatgaccta ctatgaaaat gccgtgcacg caatgtggct
     1441 ggcaagccag gaagcctgcg ataagctacc ttccgggcgt gtgtacaaca tcaccaacgg
     1501 cgagcatcgc acactgcgca gcatcgtgca gaagctgatc gacgagttga atattgactg
     1561 tcgtattcgt tccgtccctt acccgatgct ggatatgatc gcccgcagca tggagcgttt
     1621 aggccgcaag tcagcaaaag agccgccgct gacccactac ggcgcctcca agttgaattt
     1681 tgactttacg ctggatatta cgcgtgcgca ggaagagtta ggttatcagc cggtcatcac
     1741 cctggatgaa ggcatcgaga aaactgccgc ctggctacgc gaccacggaa aactgccgcg
     1801 ctaatcctgc ccgtatttct ccagcaatgc ttcggcaatc gcctgagttt ctgcatccgc
     1861 ttcgccgtta tataacgtcg ggcggaaatg catctggaat gccataatca cgcgccgctg
     1921 ctcgcgcggt gtcatatcag gttttacgtc ataaccgtag cgcgccaaaa gctccagcaa
     1981 tgacgcggta tctaccggtg tgtgcggcgc gcgcccggca aggtaaaagt tcacccgctg
     2041 cgcatccggc caggcaccaa tcccctgctg cgccagttgc tgccagggaa ataatggccc
     2101 cggatcgtct ttgcgctgcg gtgcgatatc cgcatgtgcc actacgtttt ccggcttgat
     2161 gtgataacgg gcaataatat ctttcgccag cggaataagc gcctgaatct gtgcctgttc
     2221 aaacggggca aaatatttca ctccggatga tttttgccag ccacggtttt ccagctcaat
     2281 gccaatagag gtgtcgttaa ggcgcgttgc cccgcgccag gcgctaatcc ccgcatgcca
     2341 ggccagttct tgttccggca ccagttgcca gatgcgcggt ttaccgttgt atcgcggtgg
     2401 caccgcaggg atcagataat gcgagctgac ctgcttatca gtcagtgtcg ccagcgagct
     2461 atcaaaatca tctgcggtgt agtggatcac cagcacttta atgcgcggat acgccgcctg
     2521 cgcctggtgt cgggtatcaa gctgatatcc ctctttctcg acaatgcctt tttcgcctgc
     2581 acaccctgcc agtaacagag cggcagcgac cagccagaag actcttctca tcgacgcgtt
     2641 ttcaccgccg taccgctgac gctaaccatc agcatactgc cgttttgccc gaccgtttcg
     2701 tagtcgatat caataccaac tacggcatcg gcccccagcg cccgcgcctg ggagcctaat
     2761 tcctcaaagg cgatctcccg tgctttacgc agttcttttt catacgcacc ggaacgtccg
     2821 ccaacgatat cgcggatacc ggcaaagaaa tcacggaaaa tattggcacc taaaatcgct
     2881 tcgccggtca ccacaccgca atattcaacg atggtcaggc cttccagagt tggggttgta
     2941 gaaaattgca tgatcgatct ccttgtttgt taagccttcg atctctaaag cattatcgga
     3001 ctgatatgcc atgttgggaa gaaggttttg atcagtttag gaaatcatct cattgtgtca
     3061 aaaacaccca cttaagtctt tacgattatc cgttgcataa cccgatattg gtttcattat
     3121 agggtaccgc ttacctgtct tattatgttg caccaacaac attaatgagc ggaaagaggc
     3181 aacttatatt gtaaatgact cctaaagtct tccccatctt gtgactgagg tctataccgc
     3241 tggattgctt tttggtcagc cgccgcaaaa caagaaagta ataagatctc aatgcttttg
     3301 tcattaatat aagcgaagat cattcttgca aactcgtata taaacgcccg atcctgagcg
     3361 cttaatagtt taatacaata tttattttta agagagaaaa taatttcaag gaaagcatcc
     3421 gcagcaacct tcacgttatt cccggcg
//
LOCUS       DAZXUK010000327         3432 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZXUK010000327 DAZXUK010000000
VERSION     DAZXUK010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.328"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..77
                     /locus_tag="ACH3L8_003882"
     CDS             <1..77
                     /locus_tag="ACH3L8_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFT4086781.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            435..1493
                     /gene="rfaQ"
                     /locus_tag="ACH3L8_003883"
     CDS             435..1493
                     /gene="rfaQ"
                     /locus_tag="ACH3L8_003883"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFT4086782.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1490..2613
                     /locus_tag="ACH3L8_003884"
                     /pseudo
     CDS             1490..2613
                     /locus_tag="ACH3L8_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            2606..3403
                     /gene="rfaP"
                     /locus_tag="ACH3L8_003885"
     CDS             2606..3403
                     /gene="rfaP"
                     /locus_tag="ACH3L8_003885"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFT4086783.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT          898 a          751 c          843 g          940 t
ORIGIN      
        1 cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt catcgggcat tatctgaaca
       61 taaaacacta tcagtaagtt ggagtcatta cccgtttttg acttaaatag caatttaccc
      121 gccaattctt gttgaacatc tatacttttt ttgtattttt atgcgattca ttgatttctg
      181 ctgtacgtgc aggaaggaat cgcagagcaa cagtgaaaga atatggttgc taatgatttt
      241 atttgattga tttctttatt gaaaatcaac aatgtgctta aaaatcattc gggtcggatt
      301 gccgcgaaag aagtgataca ctagcgcgtc aatatcagtg cggtatcagt attcaggtag
      361 ctgttgagcc tggggcggta gcgtgctttt tctgcttcac cttaatcgga taatctcaac
      421 aaaaagagtt acttgtggat aagccatttc gaagaatttt gctcattaag atgcgttttc
      481 atggggatat gttattaact actcccgtca ttagttctct gaaaaaaaat taccctgacg
      541 caaaaatcga tgtgctgctt tatcaggaca ccatcccgat cctgtctgaa aatccagaga
      601 ttaacgcgct ctacggcata aaaaataaaa aagcaaaagc ctcagaaaaa attgccaact
      661 tttttcatct catcaaggta ttacgtgcca ataagtatga ccttatcgtc aatcttaccg
      721 atcaatggat ggttgctata ctggttcgct tattaaatgc tcgtgtgaaa atttcccagg
      781 attatcatca tcggcagtct gctttttggc gtaaaagttt cacccatttg gtgccgttgc
      841 agggtggaaa tgtggtggaa agtaacttat ccgtgctgac accattggga cttgattcgt
      901 tggtgaagca gacaaccatg agttacccgc ctgcaagctg gaaacgtatg cgtcgcgaac
      961 ttgatcacgc tggtgttgga caaaattatg tggttatcca acctacggcg cggcaaatct
     1021 tcaaatgctg ggacaacgcc aagttttccg ctgtgattga tgccttacat gctcgtggtt
     1081 atgaagtcgt tctgacgtcc ggcccagata aagacgatct ggcctgcgtc aatgaaattg
     1141 cgcagggatg ccagacgcca ccagtaacgg cgctggctgg aaaggtgacc ttcccggaac
     1201 ttggtgcgtt aatcgatcat gcgcagctgt ttattggcgt tgattccgca ccggcgcata
     1261 ttgccgctgc agttaatacg ccgctgatat cgctgtttgg cgcaacagat catattttct
     1321 ggcgtccctg gtcaaataac atgattcaat tctgggcggg agattaccgg gaaatgccaa
     1381 cgcgcgatca gcgtgaccga aatgagatgt atctttcggt tattccggcg gcagatgtca
     1441 ttgctgctgt cgataaatta ctgccctcct ccacgacagg tacgtcgtta tgatcgttgc
     1501 tttttgttta tataaatatt ttccctttgg cggtttgcag cgcgatttta tgcgtattgc
     1561 tcagacagtc gccgcccgag gtcatcatgt tcgggtttat acccagtcgt gggaaggcga
     1621 atgccctgat gtatttgaag tgatcaaagt gccggttaaa tcgcatacca atcacgggcg
     1681 caatgcggag tattttgcct gggtgcaaaa acatttacgc gaacatcccg tcgataaagt
     1741 cgttggattc aacaaaatgc cggggctgga cgtttattat gccgctgatg tttgttatgc
     1801 cgagaaagta gcgcaggaaa aaggcttttt ctatcgcctg acgtcacgtt atcgccatta
     1861 tgccgccttt gagcgggcaa ccttcgaaca gggcaagccg acacagctgc tgatgctgac
     1921 agataagcaa atcgccgatt tccagaaaca ttatcagact gaagcggagc gttttcatat
     1981 tctgccaccg gggatttatc ctgatcgtaa atatagccag cagccagcca atagccgtga
     2041 aatcttccgt aagaagaatg gaataaccga acaacaatat ttattgttgc aggtcggttc
     2101 agacttcacg cgtaaaggtg ccgatcgttc cattgaagca cttgcttcgt taccggattc
     2161 gctgcgccac aacacattgc tatatgttgt tgggcaggat aaccgcgaaa atttgaggca
     2221 ctggcagaaa aacgcggcgt gcgcagtaat gttcacttct tctcggggcg caacgatgtc
     2281 tcggaattaa tggcggcggc ggatttatta ctgcatcctg cctaccagga agcggcggga
     2341 attgtgctgc tggaagcgat tactgcagga ttaccggtac taacaactgc cgtttgtggc
     2401 tatgcgcatt atattgtcga cgctaattgc ggcgaggcta ttgctgagcc attccgccag
     2461 gaaacattga atgagatttt acgcaaagcg ttaacgcaat cttcattgcg ccaggcttgg
     2521 gcggaaaatg cgcgacatta tgctgataca caagatttat acagtctgcc agagaaagcg
     2581 gcggatatca taacgggtgg tctggatggt tgaacttaaa gagccgtttg ccacgttatg
     2641 gcgcggcaaa gatccttttg aggaagttaa aaccttgcag ggtgaggtat ttcgtgaact
     2701 ggaaactcgc cgtactctgc gctttgaaat ggcgggaaaa agctattttc tcaaatggca
     2761 tcgcggcacg accctgaaag agataatcaa aaatttactc tcattgcgga tgccagtatt
     2821 aggcgcagac cgcgaatgga atgcgattca tcgactgcgg gatgtcggcg ttgatactat
     2881 gtatggggtg gcattcggcg aaaaaggcat gaatccgctg accagaacct cgtttattat
     2941 taccgaagat ctgacaccaa ccatcagtct ggaagattac tgtgctgact gggcgaccaa
     3001 cccaccagat gttcgcgtaa agcgtatgct tattaagcgt gtcgcgacga tggtgcgcga
     3061 tatgcatgct gcgggcatta accatcgtga ctgttatatc tgtcatttcc tgctgcactt
     3121 gcctttttcc ggtaaggaag aggagttaaa aatttcggta attgacctac accgggcgca
     3181 gcttcgcacg cgcgttccag gtcgttggcg cgataaagat cttatagggc tttatttttc
     3241 ttcaatgaat attggcctga ctcagcggga tatctggcgc tttatgaagg tgtattttgc
     3301 cgccccgctt aaagacattc tcaagcagga acaaggactg ctgtcgcaag cagaagcaaa
     3361 agccacaaaa atcagggaaa gaacgattcg aaaatcgttg taacccgtaa cttatttttg
     3421 ccaaaatttt gg
//
LOCUS       DAZXUK010000328         3415 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZXUK010000328 DAZXUK010000000
VERSION     DAZXUK010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.329"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..239
                     /locus_tag="ACH3L8_003886"
     CDS             <1..239
                     /locus_tag="ACH3L8_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFT4086784.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            249..887
                     /locus_tag="ACH3L8_003887"
     CDS             249..887
                     /locus_tag="ACH3L8_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFT4086785.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            890..2053
                     /locus_tag="ACH3L8_003888"
     CDS             890..2053
                     /locus_tag="ACH3L8_003888"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFT4086786.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2137..>3415
                     /locus_tag="ACH3L8_003889"
     CDS             2137..>3415
                     /locus_tag="ACH3L8_003889"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFT4086787.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          760 a          804 c          987 g          864 t
ORIGIN      
        1 cattgttagg ttacgttatc ccattatcca gtgcggcaat caatgcggtg agtattccag
       61 actatttcgc ctggggcggg attgcactgg tgattcagtt actcgttttt gctggtgtca
      121 gactttatat gcccgcatta agcgaaaaaa ttattaatca caataccgca gcaggaatgt
      181 ttatgggaac cgccgcgctg gctggcggta tttttaacgc agcttgtatg acatggtaat
      241 ggacgatcat ggccagaaaa cgcaaatcaa gaaataacag taaaattggt cacggagcga
      301 tcagtcgcat tggtagaccg aataatcctt ttgaaccgcg tcgcaatcgc tacgcacaaa
      361 aatacttaac gttggcgcta atgggcggtg ccgctttttt cgtattgaaa ggttgtagcg
      421 atagtagcga tgtcgataac gacggcgacg gaacatttta cgcgacagtg caggattgta
      481 ttgatgacgg taataatgcc gacatttgcg cgcgtggctg gaacaacgcc aaagcggcat
      541 tttatgccga tgttccaaag aatatgactc agcagaactg tcagtctaag tacgaaaatt
      601 gctactacga caatgttgaa cagagttgga tcccggtcgt ttctggattt ttgttaagcc
      661 gggttattcg taaagatcgc gatgagccgt tcgtctataa cagcggcgga tcctcatttg
      721 cttcccgccc cgtctggcgc aacacttccg gtgattactc ctggcgcttc ggttctggca
      781 aaaaagagtc ttactcttca ggcggcttta ccaccagaaa agcgtcgacc gtttcgcgcg
      841 gcggctatgg tcgttcttcc agcgcccgtg ggcattgggg aggctaatca tgctgagaca
      901 caacgttcct gtgcggcggg atctggacca gatcgccgct aacaacggtt tcgactttca
      961 tatcatcgac aatgaaatct attgggacga gagtcgggct taccgtttta ctctgcgcca
     1021 gattgaagag cagatcgaaa aaccgactgc ggaactgcat cagatgtgcc ttgaggtggt
     1081 ggatcgcgcg gttaaagacg aagagatcct gacgcaactg gcgatcccgc cgttgtactg
     1141 ggatgtgatc gccgaaagct ggcgcgcacg cgatccttcg ctgtatggcc ggatggattt
     1201 tgcctggtgt ggcaatgcgc cggtgaagct gctggagtac aacgccgata cgccgacctc
     1261 gttgtatgag tcggcttatt tccagtggtt gtggctggag gatgcccggc gcagcggcat
     1321 tattccgcgt gatgccgatc agtacaacgc cattcaggaa cgcctgattt cgcgctttag
     1381 tgagctttac agtcgggaac tgttttattt ttgctgctgt caggacaccg atgaagacag
     1441 gagtaccgtg ctgtacttgc aggactgcgc ccagcaggca gggcaggagt cgcggtttat
     1501 ctacattgaa gatctcggtt tgggcgtcgg cggcgtactg accgatcttg atgataatgt
     1561 catccagcgt gcatttaagc tgtatccgct ggagtggatg atgcgtgacg ataacggtcc
     1621 gctgctgcgc aagcgtcgcg agcaatgggt ggagccgtta tggaaaagta tcttgagtaa
     1681 taaagggcta atgccgctgc tttggcgctt cttccctggt catcctaacc ttcttgcatc
     1741 ctggtttgag ggtgaaaaat cgcagattgc cgctggcgaa agctatgtgc gtaaaccgat
     1801 ctactcgcgc gaaggcggaa acgtcaccat tttcgacggt cagaataacg tcgttgacca
     1861 cgctgatggt gattacgccg atgaaccgat gatctaccag gcgtttcaac ctctgccgcg
     1921 atttggcgat agctacacac tcatcggtag ctggattgtc gatgatgaag cgtgcggaat
     1981 ggggatccgt gaagataaca cactgatcac caaagacacc tcacgtttcg ttccgcatta
     2041 cattgctgga taagaatgtt ttagcaatct ctttctgtca tgaatccatg gcagtgacca
     2101 tactaatggt gactgccatt gatggaggga gacacagtgc actggcaaac tcacaccatt
     2161 tttaatcaac ctataccatt aaataacagc aacttatacc tgtctgatgg cgcgctttgc
     2221 gaagcggtaa cgcgtgaagg tgctggctgg gatagcgatt ttctagccag tattggtcag
     2281 cagttaggaa cggctgaatc ccttgaactg gggcggctgg cgaatgtaaa cccgcctgaa
     2341 ctgttgcgct acgatgccca ggggcggcgt ctggacgatg ttcgtttcca tcccgcatgg
     2401 cacctgctga tgcaggcgtt atgtaccaat cgggtgcaca atcttgcctg ggaagaagac
     2461 gctcgctccg gcgcatttgt ggcgcgcgcg gcgcgtttta tgttacatgc gcaggttgag
     2521 gctgggtcgt tatgtccagt aacgatgacc tttgccgcca cgccattgtt gttacagatg
     2581 ttacccacgc cgtttcagga ctggaccacg ccgctgctga gcgatcgcta cgattctcac
     2641 ttattgccag gtgggcaaaa acgcggtttg ttgattggca tgggaatgac ggaaaagcag
     2701 ggcggttccg atgtcatgag caacaccacc cgtgcagagc gtttggaaga tggctcttat
     2761 cggctggtgg ggcataaatg gtttttctcg gtgccgcaaa gcgatgcgca tctggtgctg
     2821 gcgcagaccg cgggcggtct gtcctgcttt tttgtgccgc gctttttgcc tgacgggcaa
     2881 cgcaacgcga ttcgcctcga gcggctgaaa gataagctgg gtaatcgctc taacgccagt
     2941 tgcgaagtgg agtttcagga tgccattggc tggttattgg ggcaggaagg ggaaggaatt
     3001 cgtctgatcc tgaaaatggg ggggatgacg cgttttgatt gcgccctggg tagccatgcc
     3061 atgatgcgcc gtgcattttc gctggcgatt tatcatgcac atcaacgcca tgtttttggt
     3121 aatccattga tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt
     3181 gaagggcaaa cggcgttgct gtttcgtctt gcgcgagcgt gggaccggcg tgccgatgcc
     3241 aaagaagccc tgtgggcgcg tttatttacg cctgcggcaa aatttgtgat ctgcaaacga
     3301 ggtatgccgt ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag
     3361 agcgagctgc cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggtt
//
LOCUS       DAZXUK010000329         3413 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZXUK010000329 DAZXUK010000000
VERSION     DAZXUK010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.330"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(279..482)
                     /locus_tag="ACH3L8_003890"
     CDS             complement(279..482)
                     /locus_tag="ACH3L8_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086788.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(484..639)
                     /locus_tag="ACH3L8_003891"
     CDS             complement(484..639)
                     /locus_tag="ACH3L8_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFT4086789.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(842..1249)
                     /locus_tag="ACH3L8_003892"
     CDS             complement(842..1249)
                     /locus_tag="ACH3L8_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4086790.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1326..1553
                     /locus_tag="ACH3L8_003893"
     CDS             1326..1553
                     /locus_tag="ACH3L8_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFT4086791.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1537..1959
                     /locus_tag="ACH3L8_003894"
     CDS             1537..1959
                     /locus_tag="ACH3L8_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFT4086792.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            2037..2814
                     /locus_tag="ACH3L8_003895"
                     /pseudo
     CDS             2037..2814
                     /locus_tag="ACH3L8_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2821..3413
                     /locus_tag="ACH3L8_003896"
                     /pseudo
     CDS             2821..3413
                     /locus_tag="ACH3L8_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          882 a          752 c          918 g          861 t
ORIGIN      
        1 taatgggtta agtgataaca gatgtctgga aatatagggg caaatccact gagccagagg
       61 attacgaagg gcttcataaa tatatgctct cactaggatt caggagaacg atcccccatg
      121 gagatggttc gtataatcaa ctcccggacg gaacttatgt ttccgaaaag ggcggtgata
      181 tttatcaaat tcgcagccag atatctgact atgcagaccg actatccggg tatcgcgcgt
      241 ctgtttttgt ttgcgaattc agtcaatgcg catggtattt ataccccgcc aagacccggt
      301 gaatatcctg cacggccttc tgctttgtag aaggcttctt cgctatcata atcgccagat
      361 tccagcgctt ctttagccag ccagatgcgt gccccagtgc ctgcatttgg ctccagttgc
      421 tggaggcgtt ttgcatcttc taggagtaga gcgataacgt gttttaattc tgtctcgttc
      481 attttactca cctgaatgtc ttcccaacca acgacgtgcg ccagcttcgg ttttaaacgt
      541 tttgcttttg gtatacgtta tggcggtgaa tgtgccgtcc tggttgggga acacgccgta
      601 caccggagat tcgttgttgc caagatcgat agtattcatg ttgaccccat ttccccttaa
      661 cgccggggta gcggaactgt ttgctgagaa caccgtgcgg tgtcttgatg aataaaattt
      721 agaataacct aaggagggtg atcaagattt ttgtgtagaa aaacctaagt tttttgatgt
      781 aaaaaacaca agtgtttgaa agtttgtgct ttttattaca gggtgtggag aaaaaagggg
      841 attatttgtt tgcgcttctt ttgcgagctt tgagtagttc ttcaaaaagt ttgttgaaat
      901 tttcaactcg agcacgcatc tctgacaaca gggccttttg ctctgactca ggcagtgcgt
      961 cgaacagttg aagtaactct ttttgatctt ctgtcagaat ggctggctga ttatccggga
     1021 tcggttcgcc tggttgttta tcttcatccc caaaaagaag ccaagtcggt gagcactgaa
     1081 gcgcttggct cagtgcgaat aatcttttcc ccgccggctg tgtttcatct ctttcccatt
     1141 gagaaattgt tacgtgagcc actttgacca gcttacctaa tgcggcctga gacagtttta
     1201 attttttacg cctatgtaag aggcgagcac cgaaggtttc gtttttcata ttagggaatt
     1261 ctaatttttc ttgacttagg tttctctacg gcctggtttc cttaggaaaa tctaaggagc
     1321 tcgatatgtt gaaaattgat gctatagcgt tttttggcag caaaacaaag cttgccaatg
     1381 ccgcaggagt taggctggca agcattgctg catgggggga actggttcct gaaggtcgcg
     1441 cgatgcgcct gcaagaggca tctggcgggg aacttcagta cgaccccaaa gtttatgacg
     1501 aatatcgtaa ggcaaaacga cctgggaagg tgattcatga aaatcaggca tgagcacatc
     1561 gaatcagtgc tgttagccct ggcagccgaa aaagggcagg cgtgggtcgc taacgcaatt
     1621 actgaagaat atctgcggca ggggggcggc gaattgtctc tggtaccagg caaggactgg
     1681 aacaatcagc agaatatcta tcaccgttgg ttgaaaggtg aaacgaaagc gcaaagggaa
     1741 aaaattcaga aactgatccc tgcggttctg gcaattcttc cgcgtgagct gcgtcaccga
     1801 ctctgtatct tcgataccct ggaacgccgt gcattactgg cggcgcagga agcgttgagt
     1861 acggcaattg atgcgcatga tgatgctgtc caggccgttt accggaaagc acatttcagc
     1921 ggtggtgggt cgcccggcga ttctgtcgta gtgcattgat tgaaattaat cgtgccggat
     1981 tgttttgttc ggtatcagtt aaatgtaacg ctgcgagcgt tacaaggtga aaacaaatgg
     2041 cttcaaactg gataaagctc gaggttatta cgccggataa gccggaaata ttcaggcttg
     2101 ctgagattct gaatattgat ccagatgccg cattagggaa ggttattcgc ttctgggcat
     2161 gggcggatca acaaatgata gacggtaatg cagattgtaa cgctcgcggc gttacaaaaa
     2221 gtgcaataga tcgcatcact tttatgtctg gttttgctga tgcgttaatt caggttggat
     2281 ggctggtcga aaatgacggt gggctttctc tacctaactt tgaacgtcat aacggaaaaa
     2341 gctctaaaaa acgggcggtt acaaacgagc gagtaacaaa aatacgcgaa ctgaaacgaa
     2401 aaggtaacgc tgccagcgtt acacaaacgg atcaaaaagc gttaccagtg gaagaggaag
     2461 aggaagatct aaatactgat ctccccctaa atccccctcg ccaaaaacga gcgtctaaaa
     2521 aattcgagcc ggaggctatt gagctgcccg attggttgcc ggaaacactc tggcatgagt
     2581 gggtccggtt cagacaggca ttgcgaaaac cgattcgaac ggagcagggc gatacgggaa
     2641 ctggaaaaat tccgtcagca gggttttaca cctgagcagg tgattcgaca cagcatcgcc
     2701 aatgaatacc agggcctgtt cgcgccgaaa ggtgttcggc ctgagacgtt gctccgacag
     2761 gttaacaccg tctcgttgcc ggacagtgcg atcccgccag gcttcagggg gtaacggacc
     2821 atgaaaaata ttgcgacagg cggcgttctg gaacgcatcc gccgactgac cccgcaacat
     2881 gtaaccgccc cattcagaac ggtagcggag tggcgcgagt ggcaacttgc tgaaggccag
     2941 aaacgtagcg aggagatcaa ccgcctgaat cgccagttgc gggtggaaaa aattctgaat
     3001 cgctcaggca tccagccgtt gcaccgtaaa tgctcgtttg cgaattacca ggtgcagaac
     3061 gacggccagc gatacgcgtt aagccaggcg aaatccatcg ccgatgaact gatgaccggg
     3121 tgtacaaatt ttgcgttcag cggaaaacct gataccgggt agaatcactt agcggcagct
     3181 atcgggaatc gcctgctgaa agacggtcag acagtgattg tggttaccgt ggctgatgtt
     3241 atgagcgccc tgcacgccag ctatgacgac gggcagtcag gcgaaaaatt tttgcgggag
     3301 ctgtgcgaag tggatctgct ggttcttgat gaaattggca ttcagcgcga gacgaaaaac
     3361 gagcaggtgg tgctgcacca gattgttgat cgccggacag cgtcgatgcg cag
//
LOCUS       DAZXUK010000330         3392 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZXUK010000330 DAZXUK010000000
VERSION     DAZXUK010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.332"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            13..132
                     /locus_tag="ACH3L8_003897"
     CDS             13..132
                     /locus_tag="ACH3L8_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFT4086793.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            167..511
                     /locus_tag="ACH3L8_003898"
                     /pseudo
     CDS             167..511
                     /locus_tag="ACH3L8_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(883..1149)
                     /gene="minE"
                     /locus_tag="ACH3L8_003899"
     CDS             complement(883..1149)
                     /gene="minE"
                     /locus_tag="ACH3L8_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFT4086794.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1153..1965)
                     /gene="minD"
                     /locus_tag="ACH3L8_003900"
     CDS             complement(1153..1965)
                     /gene="minD"
                     /locus_tag="ACH3L8_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFT4086795.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1989..2684)
                     /gene="minC"
                     /locus_tag="ACH3L8_003901"
     CDS             complement(1989..2684)
                     /gene="minC"
                     /locus_tag="ACH3L8_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFT4086796.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3204..>3392
                     /locus_tag="ACH3L8_003902"
     CDS             3204..>3392
                     /locus_tag="ACH3L8_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFT4086797.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
BASE COUNT          854 a          788 c          809 g          941 t
ORIGIN      
        1 tgcgtgtaaa aaatgcctct ttcttatgcg ggatatcatc atttcatcat gatgtctttg
       61 gtgagcggtg aacacaatac acctgcgctg tctcttcagg atgaatcccc tggtctggtg
      121 cctgcgggct gatgttgcag cagagcttag gtcacttaga cgctactatc atttatccaa
      181 tggcatggaa tcgaaatcag tcgatacccg cagtatatat cgtgaactgg gtgcaacgct
      241 gagttacaac atgcgtctgg ggaacggtat ggaaattgaa ccctgcctga aggcggctgt
      301 gcgcaaagaa tttgtcgatg ataatcgggt gaaagtgaat agtgacggta attttgtcaa
      361 tgatttgtcg ggcagacgtg gaatatacca ggcaggtatt aaagcctcat tcagcagtac
      421 gttaagcggg catttcgggg tggggtatag ccatggtgcc ggtgtggagt ccccgtggaa
      481 cgcggtggct ggtgtgaact ggtcgttctg accatcaacg attaaactgc gcttcggcgc
      541 agttttcgtt tacaggatgt tgaaagagaa aattctgggg caaaaaaagc ccgccagtta
      601 cggcgggaaa cctcatccta tgggagaaca atgaataatg aaattgcggg gttatcatct
      661 cccagtatat acacactaat aacatggtta tttactcaac caggcataaa cattttgttc
      721 tgtgcgtggg aacagcctta aggtgtaaag ggggaggtgg aaatagcaat gaggagtatc
      781 agcaagaata ctcgccgctt taccacaacg tggatgagag ggatgaaaaa ctcaaggcag
      841 aaatgactct gccttgaaga taaatgcgtt tttacagcgg gcttatttca gctcttctgc
      901 ttccggtaag gtcacgttca gctcaagaat agaaatatcg ccatcttttt gctcaagctg
      961 tacggttacc atctcaggat caatttgtac gtatttacaa atgacctcaa gaatatcttt
     1021 acgcaactgc ggcagataat gcggttctgc atcgctgcga cggcgttcag caacaataat
     1081 ctgcagccgt tcttttgcaa tgttggctgt gttcttcttc cgcgagagaa agaaatcgag
     1141 taatgccata acttatcctc cgaacaagcg tttgaggaag cctttcttct cttcttcaat
     1201 gaagcggaaa ggacgttctt ctcccaacag acgttctacg gtatctgcgt aggctttacc
     1261 cgcatcggcg ttaatgtcga gaatgaccgg ttcaccctgg ttagaggcgc gcaatactga
     1321 ttgatcctct gggatcacgc cgacgagttt gatgcgcagg atctccagca catcttccat
     1381 gctcagcatg tcacctctgc ttacgcggcc tgggttatag cgcgttaaca gcaggtgctc
     1441 tttaataggc tcttcgccat tttctgcgcg gcgtgatttt gacgccagaa tgcctaaaat
     1501 acggtcagag tcgcgtactg aggagacttc cgggttggtg gtaataatgg cttcgtctgc
     1561 aaaatagagt gccattaacg caccggtttc aatccctgcc ggggagtcac aaacgataaa
     1621 ttcaaaatcc atcgctttca gatcatcaag aactttggcg accccttcac gggtgagggc
     1681 atctttatcg cgtgtttgcg atgccggaag aatatagaga ttttcagtac gcttatcttt
     1741 aattaacgcc tgatttagcg ttgcatcgcc ctgaatgacg ttgacgaaat cgtaaacgac
     1801 ccggcgttcg caacccataa tcaggtcgag attacgcagg ccgatatcaa aatctatcac
     1861 gacagttttc tttcccttct gggccaagcc agtggcgatg gccgcgctgg aggttgtctt
     1921 accaacaccc cctttacccg aagtaacaac aataatgcgt gccatagaaa ttccttgtta
     1981 aaaagggatc aatttaacgg ttgaacggtc aaagcgtttt cgactaactg cagtcgcgcc
     2041 gctttgccat aaaattctgc tgggatttga tcactcagcc agtattcacc tgcgatggac
     2101 accaattccg ccatcaggtt cgtacaaaat atttgcgttt cccggtcacc acttgcccct
     2161 gccagcgcac gaccgcgcat catgccatag acatgaatgt tcccatcggc aatcaattcg
     2221 gccccagcgc taacgtggct tgtaacaatc agatcacatt gtggagcata aatacgctga
     2281 ccggaacgca ccggggtatc tattaaacgc gtttttgtga ccggcattgt attttgcgct
     2341 ggagcctgcg gtgcgggagc tggacgtggc gctttttctt ttccttccgt caggataggc
     2401 agccccattt tttcaatttc ggctttaagt tgcgcatctt tgcagccact tacgccaata
     2461 acccgcaaac cggttgccga aaccgcctta tgcatcgctg accagtttac cgggtcttcc
     2521 agtgcactga cgttgagtac aacgggggca tgttttaaaa atgtgggggc ctgagcgatt
     2581 ttgtctttca gcgcctgatg gataacctta ggttctgcct catgcagatg aaccacagat
     2641 aaagtgaagc tactgccttt aagctcgatt ggagtgtttg acatcctggc cttactcaat
     2701 tagctattaa tcatcgccag cgcgcgatga tgttccgaag actataaggc atgttatagt
     2761 ctggattata ttgaggcaag tcaccctccc atttattcag agtaaaagtc tattctgtga
     2821 taaatggcgc tgattcatag cttaaaaaat acccttgtca atcaacccat tgccgtcgta
     2881 cttttgattc ttcttattta cgcttccttt ttccgcaccc taatcgtgac agcatctcca
     2941 gacctgttta aatatccagt attgaggcaa gtgtattttt tcaccacgcc aggctttctt
     3001 aactttccat tttttgcagc atcattaaag aacagatacc cgttatctta atatccgcca
     3061 cagaattgct ctatcataga aaccgcagaa gacgtctgat acttctgtca ggtcatcgta
     3121 actcgtccag gtcagcggtt ttgattacgg attgatgacg aaatgagcat aagtgttgta
     3181 cggttggatt atcaggatgt gctatgaata tgatgcgtat tatttatatc ggattgtcag
     3241 gtgtggggat gatgttctca tcaatggcgt ctggacatga cgctggcgga cttcaatctc
     3301 cgacgtgcgg cgttgtttgc gacccgtata tttgtgtgaa ctcagatggc atttccccag
     3361 agttaacaag gaaatatctc ggcgaaaaag cc
//
LOCUS       DAZXUK010000331         3359 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZXUK010000331 DAZXUK010000000
VERSION     DAZXUK010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.333"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..150)
                     /locus_tag="ACH3L8_003903"
     CDS             complement(<1..150)
                     /locus_tag="ACH3L8_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HFT4086798.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATF"
     gene            complement(256..1272)
                     /gene="phnD"
                     /locus_tag="ACH3L8_003904"
     CDS             complement(256..1272)
                     /gene="phnD"
                     /locus_tag="ACH3L8_003904"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFT4086799.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(1297..2085)
                     /gene="phnC"
                     /locus_tag="ACH3L8_003905"
     CDS             complement(1297..2085)
                     /gene="phnC"
                     /locus_tag="ACH3L8_003905"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFT4086800.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(2218..2661)
                     /gene="yjdN"
                     /locus_tag="ACH3L8_003906"
     CDS             complement(2218..2661)
                     /gene="yjdN"
                     /locus_tag="ACH3L8_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFT4086801.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(2819..3154)
                     /locus_tag="ACH3L8_003907"
     CDS             complement(2819..3154)
                     /locus_tag="ACH3L8_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFT4086802.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT          713 a          856 c          935 g          855 t
ORIGIN      
        1 gaaggttgcc atgttgccgc cgtctttaat cagcgtaagc ggggccattt ccgcgccctg
       61 ccacgagacg accagcacgt cgagaacaac ggcccagctc agaagcgaga accagctacg
      121 cttgggtggg gcgatggtga tggtttgcat gtttggctcc ggtctgtagg ccggataagg
      181 tgcttgcacc gcatccggca tcaacgccta cacattgtcg gatgcgacgc cggggcgtct
      241 tatccgacct acgatttact gcaccgcttt actcaccgaa ctcatcgcgc ttaacgcgtt
      301 gttcaggcgg tccaggtcat ccagctgcgc ctgaatctcg gtggttttcg ccagcttgtc
      361 ctgctcattc agccctttat tgcctttcac gctttgtatc tctttaaaca gtgcgagctg
      421 gcgaatcggc accagttgca ggtcgctgga agcgcggaac ggcgcccagc ccaggcgttc
      481 cagcaccgct ttctcttccg gcgtttttcc gtagttcata aagaagtcgt agatcttgtc
      541 tttggtagtt tcggacagat tcttgcgcca gacgatcgga tcgcctagga tcagcggcga
      601 tttccagatc accttcagct ctttaagctt ctccggcgcg gaggttttca gcttgtcgag
      661 gttttcggtg ttgttggtgg caacatccac ttgcttgttg gcgacggcca gcgcgttggt
      721 ttcatgcccg gcgttgacgg tgcgcttgaa gtcgctggcg gagatattgt ttttagcgaa
      781 gacgtagtaa ccggggacga ggaagccaga ggtggagtta ggatcgccat tgccgaaggt
      841 gagatctttc cgcttcgcca gcagatcgtt caggttgttg atcgggctgt ctttgttgac
      901 gatcaacacg ctccagtaac ccggcgatcc atccgccgcg accgtctggg cgaagacctg
      961 gccattggcg cgatccaccg cttccatcgc cgacaggttg ccgtaccagg caatatccac
     1021 tttattgaag cgcatcccct ggataatgcc cgcgtagtcc ggggcaaaga aggcgttgac
     1081 cttcacgccc agtttcttct ccatatcctg taaaaacggc gtccattgcg gtttcaggtt
     1141 ttgctgtgat tccgttgaaa taatgccgaa attcagcgcc ttttcctgct cttcggcgtg
     1201 tgccggggtt aacagggtgc tgaggctgaa catgctggtg aaggccagcg aggcaattat
     1261 cttagcgttc attcgttttc ctcaatgatg gggatatcag gcagctttcg cgttctcttc
     1321 gatgcggtta atgctgcggt agagatggtc aaaacgttcg ttatcaaaca gttggctgct
     1381 gccgtcgtag aagacgtgcc cctgacgcag ggcgacgatg cgttcgcagt agcgcagggc
     1441 gtaatccacc tgatgcagcg tgacgaccac ggtgatgccg tcgttctggt tgatgtcgcg
     1501 cagggtgtcc atcacgatgc gcgctgattc cgggtccagc gaggcgatgg gttcatcggc
     1561 cagaatcact ttcgcctgct gcatcagcgc acgggcaatc gccacacgtt gctgctggcc
     1621 gccggagagg gtggaaacgc gctgatgggc aaaatgcacc atgccaacgc gggtcagcgc
     1681 ctgtaacgcg cgctgtttct gctcgccggt gaaccagcta aaacaggtgc gccagaacgg
     1741 cgtgctgccg agcgcgccaa tcagcacgtt ctccagtacg ctcaggcggt tcaccaggtt
     1801 gaattgttga aagatgtagc cggtgttggc gcggcttttg cggatatcgc gggccaggcg
     1861 gccttcgcgc tggacagtgc ggcccagtag ctcgatatgg ctaccggcgg atttatcgcc
     1921 ggtaatcaaa ccgcttaaat gacgtaaaag ggtggatttg ccggaacccg acggcccaag
     1981 cagagccacc atttcactgt gatgaatgtt cagatcaacc gcatgcagcg cttgatgctg
     2041 attgaaggtt ttggcgagct tctcgacacg gataatcgtt tgcatgatgc agcctcccta
     2101 aaaaagtggc tccatcgtgg cggattattg tgacgatttg gttaattaaa agtgactaac
     2161 agatgaagag ttaacgggaa ttcgatgaca gtgcgggagg gcgggcctcc cggcgggtta
     2221 ttgcgttggt tgttgtttga cgacattaat catccacggt acgccaaatt tatcggtgac
     2281 tttgccaaag ccatgcgccc agaaagtttc ctgccaggcc atttcgattt ttccgttagc
     2341 ggcaaggtta tcaaaccagc gttttccttc ttcgacctgt tgcgaatcga gcaccagcgt
     2401 aaagccggag tagctggctt ttcctgacgg tatagcatcg ctcatcatga tgtcgcttcc
     2461 ggcaatgcgc acgttggcat gagcgatggc ggtatcggga aattgcattc cggaagggca
     2521 gttctcggcg ctgtcctgcg ctgatttcgg catttcgccg aagctgattt tatagagcag
     2581 ttccgcgccc aacgtacgtt gataataggc aatcgcgtcg gaacagttac cggcaaaaga
     2641 gaggtaggga cttaacggca tgatggtgac ctcagttaag agaagccagt taagtgtagt
     2701 tcgaaattta tacagatgag aggctgcctg ataagacgcg caagcgtcgc atcaggcagt
     2761 cggcatcgtt gccggatgcg gcgcgagcgc cttatccggc ttaccgatca caatacaatc
     2821 agttcttttt cacaaactca gatttcagtt tcatcggacc aaaaccgtcg attttgcaat
     2881 cgatgttatg gtcgccttca accaggcgga tgtttttcac tttggtgcca attttcagca
     2941 tcgaagagct acctttcacc ttcagatctt taatgatggt aacgctgtcg ccgtcagcca
     3001 gcagattgcc gttagcatct ttaacgatca gctcgtcgct ttcctgtgca ggttctgcgt
     3061 cgttccattc gtaggcacat tccgggcaga tgtacatgcc gttatcttcg taagtgtatt
     3121 cggagttgca ttttgggcag tgtggtaatg acatgtggat ttcctcaaaa gtcagcaggg
     3181 caaaagcgcc aaaaaacggc agattgccga aaaaggccgc aattatacac aaaatccctg
     3241 agtttgtcgg gggtactgct gattttagac gacgatcgtt ttcatttgaa aggaaacgca
     3301 aaaagaaaat aatctttttg cctctattta ttatttaaga taatgttaat gctctactt
//
LOCUS       DAZXUK010000332         3348 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZXUK010000332 DAZXUK010000000
VERSION     DAZXUK010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.334"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            19..897
                     /locus_tag="ACH3L8_003908"
     CDS             19..897
                     /locus_tag="ACH3L8_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFT4086803.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="ACH3L8_003909"
     CDS             894..2285
                     /locus_tag="ACH3L8_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFT4086804.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="ACH3L8_003910"
     CDS             2282..2539
                     /locus_tag="ACH3L8_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086805.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="ACH3L8_003911"
     CDS             2634..2834
                     /locus_tag="ACH3L8_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFT4086806.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="ACH3L8_003912"
     CDS             2827..3207
                     /locus_tag="ACH3L8_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFT4086807.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          885 a          726 c          969 g          768 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaac
//
LOCUS       DAZXUK010000333         3340 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZXUK010000333 DAZXUK010000000
VERSION     DAZXUK010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.335"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            182..508
                     /locus_tag="ACH3L8_003913"
     CDS             182..508
                     /locus_tag="ACH3L8_003913"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFT4086808.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            530..1777
                     /locus_tag="ACH3L8_003914"
     CDS             530..1777
                     /locus_tag="ACH3L8_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFT4086809.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            1792..2394
                     /gene="ypdF"
                     /locus_tag="ACH3L8_003915"
                     /pseudo
     CDS             1792..2394
                     /gene="ypdF"
                     /locus_tag="ACH3L8_003915"
                     /inference="COORDINATES: protein motif:HMM:NF007310.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            2355..2462
                     /locus_tag="ACH3L8_003916"
     CDS             2355..2462
                     /locus_tag="ACH3L8_003916"
                     /inference="COORDINATES: protein motif:HMM:NF019360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086810.1"
                     /translation="MARGAAARQSQRQDCCSGRVCHSRFWCAVSGLLLI"
     gene            2459..2875
                     /locus_tag="ACH3L8_003917"
     CDS             2459..2875
                     /locus_tag="ACH3L8_003917"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFT4086811.1"
                     /translation="MTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQ
                     QIDDAARRVITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIY
                     LLGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            2875..>3340
                     /locus_tag="ACH3L8_003918"
     CDS             2875..>3340
                     /locus_tag="ACH3L8_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="metalloprotein; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFT4086812.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVL"
BASE COUNT          722 a          864 c          944 g          810 t
ORIGIN      
        1 atcttttaaa tcatacagtt aattcaattt ttctttgtgt cccctcacaa ggccgacctg
       61 cgtcacactt ccgtacagcg ggattaattc tccagtaaat gcattatttg tctggtaacg
      121 gcgatttgtt ttgcacgttc ataatttcac tagtcaacta tcactgaacg aggaattaac
      181 gatgagtaag aaactgattg ccttatgtgc ctgcccaatg ggcctggctc acacttttat
      241 ggccgctcag gtgctggaag aagcggcggt agaagccggt tatgaagtga aaattgaaac
      301 ccagggcgcg gacggcatcc agaatcgcct gacggcgcag gatatcgccg aagcgaccat
      361 catcatccat tccgtggcag ttaccccgga agataacgaa cgtttcgaat cacgcgacgt
      421 ttatgaaatc actttgcagg acgcaattaa aaacgctgcg ggcatcatca aagaaatcga
      481 agagatgatt gcctctgaac aacagtaata ttcgacaggg ataattgtta tggccattaa
      541 aaaacgcagt gcaaccgttg tgcctggcgc atccggtgcg gcagcggcag ttaagaatcc
      601 gcaggcctct aaaagcagct tctggggtga actcccgcag catgtgatgt cagggatttc
      661 acgcatggtg ccgaccttaa ttatgggcgg tgtgatcctc gctttcagcc agctgattgc
      721 ttatagctgg cttaaaattc ctgctgagat cggcatcatg gatgctctta atagcgggaa
      781 attctccggt ttcgacctct ccttacttaa atttgcctgg ctgtcgcagt ccttcggcgg
      841 cgtgttgttt ggttttgcca ttccgatgtt tgctgcattt gtggcgaact ctatcggtgg
      901 caaactggcg ttcccggctg gttttatcgg cgggttgatg tctacccagc cgacgcaact
      961 gctgaacttc gatcccagca ctatgcaatg ggcgacctct tcgccggtgc cgtccacctt
     1021 cattggtgcg ctgattattt ctatcgttgc cggttacctt gtgaaatgga tgaaccagaa
     1081 aatccagtta ccggatttcc tgttagcgtt caaaaccaca tttttgctac cgattctttc
     1141 cgccattttt gtcatgctgg cgatgtacta cgtcatcact ccctttggtg gctggatcaa
     1201 cggcggtatc cgtactgtgc tgactgccgc aggtgagaag ggcgcgctga tgtacgcaat
     1261 ggggatcgcc gcggcgacag caatcgacct tggtggcccg atcaacaaag ccgcaggttt
     1321 cgttgccttc agctttacca ctgaccacgt attaccggtc accgctcgtt ctatcgctat
     1381 cgttattccg ccgattggtc tgggtctggc gaccattatt gaccgtcgtt taaccggcaa
     1441 acgcctgttc aacgctcaac tttatccgca gggtaagacc gcgatgttcc tggcctttat
     1501 ggggattagt gagggtgcga ttccgtttgc gctggaaagc cccatcaccg ctattccgtc
     1561 atatatggtc ggcgcgattg tcggctcaac cgcagctgtc tggctgggcg cagtgcaatg
     1621 gttcccggaa tccgctatct gggcatggcc gctggtcact aacttgggcg tttatatggc
     1681 ggggatcgcg ctgggggcaa tcattactgc gctgatggtg gtgttcctgc gtctgatgat
     1741 gttccgcaaa ggcaaattgt taatcgatag cctgtaagaa ggaaaatcca gatgacatta
     1801 ctcgcttcgc tgcgcaactg gcttaaggtg caacaactgg atgcggtgct tctctcctca
     1861 cggcagaaca aacagccgca tctggggatc tccaccggat caggctatgt ggttattagc
     1921 cgtgaaagtg cgcacattct ggtagattcg cgctatttcg ttgaggtgga agcccgtgcg
     1981 caaggctacc agctgcattt gcttgacgca acgaacacgc ttaccactat cgtcaatcaa
     2041 atcattgccg atgagcagtt gcaaacgctc ggttttgaag gccagcaggt gagttgggaa
     2101 accgcgcacc gctggaagtc tgaactcaat gcgaaactgg ttagcgccac gccggatgtg
     2161 ctgcggcaaa tcaaaacgcc agaggaggtg gagataatcc gcctcgcctg tgggattgcc
     2221 gatcgcggag cggagcatat tcgccgcttt attcaggcgg ggatgagcga gcgcgagata
     2281 gccgctgaac tggagtggtt tatgcgccag cagggcgcag aaaaagcctc ttttgacacc
     2341 attgtcgcca gcggctggcg cggggcgctg ccgcacggca aagccagcga caagattgtt
     2401 gcagcgggcg agtttgtcac tctcgatttt ggtgcgctgt atcagggcta ctgctgatat
     2461 gacgcgcacc ttgctggtga atggcgaagg ggtgagcgcc gaatctcacc cgctgtttga
     2521 cgtctatcag attgtcctgc aggcacagct cgcggcaatc tctgcaattc gccccggcgt
     2581 gcgctgccag cagattgacg acgccgcgcg ccgggtcatt accgaggctg gatttggcga
     2641 ctatttcggt cataacactg gtcacgctat cggcattgaa gtccatgaag atccgcgttt
     2701 ttcaccgcgg gacaccacga cgctacagcc aggcatgttg ctgaccgtgg agccggggat
     2761 ttatttgcta gggcaagggg gcgtgcgcat cgaagatgtt gtgctggtta ccccgcaagg
     2821 cgcagaagtg ctctacgcca tgccgaaaac agtgttgctc acgggagagg cataatggat
     2881 ttatcgctat taaaagcgtt gagcgaggca gatgcgatcg cctcctcgga acaggaagtg
     2941 cggcagatcc tgctggaaga agcggctcgc ctgcaaaaag aagtgcgatt tgatggtctg
     3001 ggatcggtgc tgatccgcct caatgaatcg acaggtccga aggtgatgat ctgcgcccat
     3061 atggacgaag tgggatttat ggtgcgcagc atctcccgcg aaggcgctat tgacgtgctg
     3121 ccggttggca acgtgcgcat ggctgcccgc cagctgcagc cggtgcgcat caccacccgt
     3181 gaagagtgca aaattccagg cctgcttgac ggcgaccggc aggggaatga cgttagcgcc
     3241 atgcgcgtgg acattggcgc gcgcacctat gacgaagtga tgcaggcggg aattcgtcca
     3301 ggcgatcgcg tcaccttcga taccactttt caggtcctcc
//
LOCUS       DAZXUK010000334         3325 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZXUK010000334 DAZXUK010000000
VERSION     DAZXUK010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.336"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..208
                     /locus_tag="ACH3L8_003919"
     CDS             <1..208
                     /locus_tag="ACH3L8_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086813.1"
                     /translation="RHPDKRFIFNGNEIWKRGESTTEYTRAHQRTIRRLRRLIARKQY
                     TEKWFRKYDTWSAGYYELMATKPF"
     gene            269..457
                     /locus_tag="ACH3L8_003920"
     CDS             269..457
                     /locus_tag="ACH3L8_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086814.1"
                     /translation="MNIYFRIVISLAIIACIYGLIVPALISMKDTVAVISGFALACLT
                     PPCIYAIYKGLSFSKDKR"
     gene            454..1281
                     /locus_tag="ACH3L8_003921"
     CDS             454..1281
                     /locus_tag="ACH3L8_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFT4086815.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1325..2074
                     /locus_tag="ACH3L8_003922"
     CDS             1325..2074
                     /locus_tag="ACH3L8_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFT4086816.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2071..2640
                     /locus_tag="ACH3L8_003923"
     CDS             2071..2640
                     /locus_tag="ACH3L8_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086817.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2637..2759
                     /locus_tag="ACH3L8_003924"
     CDS             2637..2759
                     /locus_tag="ACH3L8_003924"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086818.1"
                     /translation="MKIEYQDAAGGESMSWPDAIVTLGMVFAVAFVVYSICRWG"
     gene            2764..3006
                     /locus_tag="ACH3L8_003925"
     CDS             2764..3006
                     /locus_tag="ACH3L8_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFT4086819.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3010..3156
                     /locus_tag="ACH3L8_003926"
     CDS             3010..3156
                     /locus_tag="ACH3L8_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086820.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3165..>3325
                     /gene="xisR"
                     /locus_tag="ACH3L8_003927"
     CDS             3165..>3325
                     /gene="xisR"
                     /locus_tag="ACH3L8_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFT4086821.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          928 a          796 c          855 g          746 t
ORIGIN      
        1 acgccatcct gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc
       61 aaccacagag tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc
      121 caggaaacag tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta
      181 cgaactgatg gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat
      241 aaggcaagcc gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact
      301 ggcaattatc gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac
      361 ggtagcagtg atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat
      421 ttataagggt ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc
      481 cttgttctgc tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc
      541 atcaaggtaa acaaactggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc
      601 tactggacgg gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc
      661 tacgatgagc cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc
      721 gtggcctaca aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc
      781 gtggacgaca ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg
      841 gccagcaaaa tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca
      901 gctcttaaag acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca
      961 tatgtgggta agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg
     1021 gcgaaccaga aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac
     1081 atgttgcgcg cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa
     1141 gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg
     1201 gaagccatca acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca
     1261 ccatttattc aggttaagta atacatatgc ccggtattac acgccggggt gtctggagat
     1321 aaaaatgaat actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca
     1381 gcgtgttgtg acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg
     1441 tgcagcattc aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg
     1501 tgcggacgta atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg
     1561 gattgtactt tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc
     1621 ctggcaggtt cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga
     1681 aatcgagatg atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca
     1741 tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat
     1801 gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa
     1861 gtgtcggaat ctggtgaacg catatcaaat tcctacagac acccacgaat tcatgactcc
     1921 ggacggtctg ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca
     1981 ccaaatgatg tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct
     2041 tttccaggtg atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc
     2101 gctctggtgt gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg
     2161 ccgaaacata ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg
     2221 acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca
     2281 gagctgattt ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg
     2341 tccattaccg gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg
     2401 aaaaagtttg accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag
     2461 aagatgtttg gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg
     2521 caagcggtca gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt
     2581 gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga
     2641 aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa
     2701 ctctggggat ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac
     2761 cacatgttcg ctttgattca acgcgggcag atatacacag acagagccgg atacccagta
     2821 gaaattactc gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg
     2881 cgagtacgca tcggtgaatt cagtagcctg ttcgagcata ttgaccacct ggaatatcac
     2941 aagattctgg ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg
     3001 aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac
     3061 gacctgacgc gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag
     3121 gtctggtgcc actgccgtgg gctgagtgtt gtgtgaggta atgcatgggc aatgtgattc
     3181 aactggctcc caatgaatgg gtttgtgaaa gcgttctaat cgcaattacc gggctcaaac
     3241 caggcacaat tcttcggacc cggaaagaat gctggatggt tggaagagag tatattcacg
     3301 tatcaccaga cggtaatcca aagcc
//
LOCUS       DAZXUK010000335         3321 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZXUK010000335 DAZXUK010000000
VERSION     DAZXUK010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.337"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            41..116
                     /locus_tag="ACH3L8_003928"
     tRNA            41..116
                     /locus_tag="ACH3L8_003928"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:75..77,aa:Glu,seq:ttc)
     gene            310..3213
                     /locus_tag="ACH3L8_003929"
     rRNA            310..3213
                     /locus_tag="ACH3L8_003929"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          885 a          725 c         1017 g          694 t
ORIGIN      
        1 gaaagtgaaa agcaaggcgt cttgcgaagc agactgatac gtccccttcg tctagaggcc
       61 caggacaccg ccctttcacg gcggtaacag gggttcgaat cccctagggg acgccacttg
      121 ctggtttgtg agtgaaagtc acctgccttg atatctcaaa actcatcttc gggtgatgtt
      181 tgagatattt gctctttaaa aatctggatc aagctgaaaa ttgaaacact gaacaacgaa
      241 agttgttcgt gagtctctca aattttcgca acacgatgat gagtcgaaag aaacatcttc
      301 gggttgtgag gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg
      361 aaggacgtgc taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga
      421 tttccgaatg gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt
      481 aatgaggcga accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga
      541 gattccccca gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg
      601 ttagtggaag cgtctggaaa ggcgtgcgat acagggtgac agccccgtac acaaaaatgc
      661 acatactgtg agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg
      721 accatcctcc aaggctaaat actcctgact gaccgatagt gaaccagtac cgtgagggaa
      781 aggcgaaaag aaccccggcg aggggagtga aaaagaacct gaaaccgtgt acgtacaagc
      841 agtgggagca cgcttaggcg tgtgactgcg taccttttgt ataatgggtc agcgacttat
      901 attctgtagc aaggttaacc gaatagggga gccgaaggga aaccgagtct taactgggcg
      961 ttaagttgca gggtatagac ccgaaacccg gtgatctagc catgggcagg ttgaaggttg
     1021 ggtaacacta actggaggac cgaaccgact aatgttgaaa aattagcgga tgacttgtgg
     1081 ctgggggtga aaggccaatc aaaccgggag atagctggtt ctccccgaaa gctatttagg
     1141 tagcgcctcg tgaattcatc tccgggggta gagcactgtt tcggcaaggg ggtcatcccg
     1201 acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga gacacacggc
     1261 gggtgctaac gtccgtcgtg aagagggaaa caacccagac cgccagctaa ggtcccaaag
     1321 tcatggttaa gtgggaaacg atgtgggaag gcccagacag ccaggatgtt ggcttagaag
     1381 cagccatcat ttaaagaaag cgtaatagct cactggtcga gtcggcctgc gcggaagatg
     1441 taacggggct aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt gttgggtagg
     1501 ggagcgttct gtaagcctgt gaaggtgtgc tgtgaggtat gctggaggta tcagaagtgc
     1561 gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg
     1621 ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg
     1681 tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga
     1741 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
     1801 gcaaatccgg aaaatcaagg ccgaggcgtg atgacgaggc actacggtgc tgaagcaaca
     1861 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
     1921 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
     1981 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tacgtaggtg aagcgacttg
     2041 ctcgtggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
     2101 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
     2161 taattgatgg ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt
     2221 aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat
     2281 ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa
     2341 gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac
     2401 tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct
     2461 gcatggagcc gaccttgaaa taccaccctt taatgtttga tgttctaacg tggacccgtg
     2521 atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag
     2581 taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg
     2641 cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg
     2701 atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa
     2761 caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct
     2821 catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt
     2881 acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg
     2941 agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc actggtgttc
     3001 gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa gtgctgaaag
     3061 catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag ggtcctgaag
     3121 gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct
     3181 aaccggtact aatgaaccgt gaggcttaac cttacaacgc cgaagatgtt ttggcggatt
     3241 gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
     3301 agaattagcc tggcggccgt a
//
LOCUS       DAZXUK010000336         3316 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZXUK010000336 DAZXUK010000000
VERSION     DAZXUK010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.338"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(2..298)
                     /locus_tag="ACH3L8_003930"
     CDS             complement(2..298)
                     /locus_tag="ACH3L8_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFT4086822.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1241
                     /gene="tonB"
                     /locus_tag="ACH3L8_003931"
     CDS             513..1241
                     /gene="tonB"
                     /locus_tag="ACH3L8_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFT4086823.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACH3L8_003932"
     CDS             complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACH3L8_003932"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFT4086824.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1784..2323)
                     /locus_tag="ACH3L8_003933"
     CDS             complement(1784..2323)
                     /locus_tag="ACH3L8_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFT4086825.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2353..3096)
                     /locus_tag="ACH3L8_003934"
     CDS             complement(2353..3096)
                     /locus_tag="ACH3L8_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFT4086826.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3124..3213)
                     /locus_tag="ACH3L8_003935"
                     /pseudo
     CDS             complement(3124..3213)
                     /locus_tag="ACH3L8_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
BASE COUNT          952 a          819 c          793 g          752 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac
      841 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
      901 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
      961 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     1021 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     1081 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     1141 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     1201 atatcctgtt taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc
     1261 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     1321 accgctacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     1381 gctacttttt tcacccacac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     1441 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     1501 tcaacccgca cagtcactac gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca
     1561 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     1621 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     1681 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     1741 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     1801 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     1861 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     1921 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     1981 ccggttgcgg cagcgtgagt tctttactca gcatccgctg aattagcggc tttttcatca
     2041 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgta accttccatt
     2101 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     2161 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     2221 tcgccgtggc gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     2281 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     2341 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     2401 ggcagaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     2461 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     2521 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     2581 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     2641 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     2701 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     2761 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     2821 accgattaat tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc
     2881 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     2941 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     3001 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     3061 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     3121 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     3181 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaagc
     3241 gcggcaagtg gcgatcgagt taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa
     3301 tcacattaac caggat
//
LOCUS       DAZXUK010000337         3296 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZXUK010000337 DAZXUK010000000
VERSION     DAZXUK010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.339"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..602)
                     /locus_tag="ACH3L8_003936"
     CDS             complement(<1..602)
                     /locus_tag="ACH3L8_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFT4086827.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            1122..2021
                     /locus_tag="ACH3L8_003937"
     CDS             1122..2021
                     /locus_tag="ACH3L8_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFT4086828.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            2070..3083
                     /gene="yhjD"
                     /locus_tag="ACH3L8_003938"
     CDS             2070..3083
                     /gene="yhjD"
                     /locus_tag="ACH3L8_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFT4086829.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
BASE COUNT          833 a          855 c          776 g          832 t
ORIGIN      
        1 caggaggaga tatttaacat cattgccgcc tgggtgcgat ttttcacttc cagacggcga
       61 tacagggatt ccagatgcgc ttttaccgtt ccggtactga tattcagcgc tctgccgatc
      121 tccttatttg attcgcccgc cgctaacatg gttaaaatct cccgctggcg ggcgcttaac
      181 gatttgagat ctttaatgtc cttttccggc gtcgtccgcc agtctccagg cagaaacatc
      241 atccccatcg ccgcactatt taccgccaac gcaaatgtct cgacggttga atcgcgaggc
      301 acaatggcca gcacattaaa atggataact tcctgtaacc accgtttatt gcaatccgtc
      361 gccgtgatta acaccttaac ctcaggaaac tgcaccacgg ttttttgcag caaccagtag
      421 caaaactcac catcctgatc gccatcgagc ataactaagg cttcagggta actttccagc
      481 ttttgccata actcgtctgc ctgactgacc ccctgaatac tcactcctgg aatacgctgc
      541 tgtaaactga tttttattcc atgaataaat attgactgcc tgtcaaacat gactatttgc
      601 ataactgaat ctccacctga atacgttaaa aagacttaag tagtggaagg gtattacccg
      661 cgagaaaaaa taagaattcg ccatttggcg gtggccattc tacagagatg acgcgtagaa
      721 aatagttacc gatataaata gttacagcta aacgcctgaa attacatgtc gagggcacta
      781 tttaaaacaa ttttgaggat ttccttatat tggtggttag tacgcatgca attaaaaatg
      841 aaattccgcg actacaagcc aaaataacaa atggcaatga gacaaaaata agcacaaata
      901 gccaatacgt cctctattca ctttaaaggg aatcactgaa aaatacgctc tgtttaaggg
      961 gattcacctt tctcataaag ctattccgcc cttttcctgc tgagaaatcg ccacattcgt
     1021 catgacaaca ttgtgaaacc cggcattaga tgttagaaaa aacaataaac aatgcgatat
     1081 gcgcgttact gtttcgctgt gaacaacaat cggtcaaaga aatggataaa attcacgcaa
     1141 tgcagttgtt catcaaagtc gcggagctgg aaagtttttc ccgcgcggcg gatttctttg
     1201 ctttgccaaa gggaagtgtt tcgcgccaga tacaggcact ggaacatcaa cttggcaccc
     1261 agcttctcca gcgcactacg cgacgggtca aactcacgcc agaaggcatg acctattatc
     1321 aacgggcaaa agatgtgttg agtaatctca gcgaactgga cggtctgttt caacaggatg
     1381 ccaccagtat cagcggtaaa ttacgcatcg acatcccgtc aggaatcgcg aaaaacctgt
     1441 tactgccgcg tctgtcggaa tttctctatc agcatccggg aattgagctg gaactgagta
     1501 gccataaccg tccggtagat attcttcatg atggttttga ttgcgtgata cgcactggcg
     1561 cgttaccgga agatggcgtt atcgcccgtc ccctcggcaa actaaccatg gtcaactgtg
     1621 ccagtccgca ctatctgacg cgctttggtt atccgcaaag ccccgatgat ctgacttcac
     1681 acgcaatagt gcgttataca ccgcacctgg gtgtacatcc gttaggtttt gaggttgcca
     1741 gcgttaatgg cgtccagtgg tttaagtctg gcggcatgtt gacggtaaac agtagcgaaa
     1801 actatctcgc cgccggtctt gccggtctgg ggattattca gatcccgcgc attgccgtgc
     1861 gcgaagccct gcgtgccggg cggcttattg aagtgttgcc aggctaccgt gccgagccgc
     1921 tctccctttc gctggtttac ccgcagcgtc gggagctttc ccggcgtgta aacctgttta
     1981 tgcagtggct ggctggcgta atgaaagagc acctggactg accgactata ctttttaaga
     2041 atgaccacga cagaaggaca aaagagcgga tgacgcagga aaacgagatc aaacgtccca
     2101 cccaggatct ggagcacgag ccgattaagc agttggataa tagcgagaaa ggcggcaaag
     2161 ttagccaggc gctggaaacc gtcaccacca ctgccgaaaa agtccaacgc caaccggtta
     2221 ttgcgcacct tattcgcgcg acagaacgct ttaacgatcg gctgggtaac cagtttggtg
     2281 cggctatcac ctatttctca tttttgtcga tgatcccgat tttgatggtg tcgtttgccg
     2341 cagggggctt tgtgctggcc tcccatccga tgttgctaca ggatatcttc gacaaaattc
     2401 tgcaaaacat cagcgatccg acactagccg ccacgttgaa aaacaccatc aacaccgccg
     2461 ttcagcagcg tacgactgta gggcttgtcg gcctggcggt ggcactttat tccggcatca
     2521 actggatggg taacctgcgt gaagcgattc gtgcccagtc gcgcgatgtc tgggaacgct
     2581 cgccgcaaga tcaggagaag ttctgggtaa aatatctgcg tgattttatt tcactgattg
     2641 gtttgttgat tgcgctgatt gtgacgcttt cgatcacctc ggttgccggt tcggcgcagc
     2701 aaatgattat tagcgccctg cacctgaaca gcattgagtg gctgaaaccg acgtggcgat
     2761 tgattggcct ggcgatttcc atcttcgcca actatctgct tttcttctgg atcttctggc
     2821 ggttgccgcg ccaccgtccg cgcaaaaaag ccctgattcg cggaacattt ctggctgcta
     2881 ttggttttga agtgattaaa atcgtgatga cctacaccct gccatcgctg atgaaatccc
     2941 cctctggcgc agcatttggt tccgtgctgg ggctgatggc atttttctac ttcttcgccc
     3001 gtttgacgct gttttgcgcg gcgtggattg ccaccgccga atataaagac gacccgcgaa
     3061 tgccagggaa aacgcagcct taaaataggc cggatgcggc gtaaacgcct tatccggcat
     3121 acgattttat tcggcctggc tccccgtagg ccggataaga tgcgccagca tcgcatccgg
     3181 ctataatgcg cacataacct cttgaaactc ttccccagag cctcttcagc catctatttg
     3241 ggagcaaaca atttcactcc aactcataac cccagcatat aaatccagtt ggtaac
//
LOCUS       DAZXUK010000338         3274 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZXUK010000338 DAZXUK010000000
VERSION     DAZXUK010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.340"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..961)
                     /gene="glk"
                     /locus_tag="ACH3L8_003939"
     CDS             complement(<1..961)
                     /gene="glk"
                     /locus_tag="ACH3L8_003939"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFT4086830.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHI"
     gene            1165..2421
                     /locus_tag="ACH3L8_003940"
     CDS             1165..2421
                     /locus_tag="ACH3L8_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFT4086831.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            2536..2862
                     /gene="ypeC"
                     /locus_tag="ACH3L8_003941"
     CDS             2536..2862
                     /gene="ypeC"
                     /locus_tag="ACH3L8_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFT4086832.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(3003..>3274)
                     /locus_tag="ACH3L8_003942"
     CDS             complement(3003..>3274)
                     /locus_tag="ACH3L8_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HFT4086833.1"
                     /translation="LWVRRTVTMLPSFIVILMGLDPTRILVMSQVLLSFGIALALVPL
                     LIFTSDSKLMGDLVNSKRVKQTGWVIVVLVVALNIWLLVGTALGL"
BASE COUNT          780 a          881 c          824 g          789 t
ORIGIN      
        1 gaatgtgacc taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggatagtcat
       61 ggacgatgag atacaccgga atatcatgga catattcttt aaagcgccct ttatcttcaa
      121 atgcggcacg gaaaccggag gctttgaaga actcaaggaa gcgcggcacg ataccgcccg
      181 caataaacac gccgccaaat gtcccgagat tgagcgccag attgccgcca aaacggccca
      241 taatgacgca aaacagcgac aatgcgcggc ggcaatcggt gcagctgtca gccagcgcgc
      301 gttcggtaat atcttttggc ttgagatttt ctggcaggcg gttgtcagct ttcacaattg
      361 cgcgatacaa attcaccagc ccagggccag aaagcacgcg ctccgccgaa acatgaccaa
      421 tttccgcacg caatatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa
      481 cgtgaccgcc ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa
      541 ccccaagccc cgttccggca ccgtaaaccg caataggctt accttcgacc ggttctgcgc
      601 caccaaactg aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa
      661 aatcgttaat aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga
      721 acgcccaggt atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa
      781 tacagccgtc tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga
      841 ggctggggta atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat
      901 cacacagagc aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca
      961 ttcttcaact gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg
     1021 cataacaaca acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacaatta
     1081 ctttatcgtt tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat
     1141 ctgaccccgc ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta
     1201 tcgctccccg ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa
     1261 atcgcctcgg tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc
     1321 caggattcac ccctctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg
     1381 gttatccgtt tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc
     1441 gcaccggttc cgccctctgc gctacctaga cttatcgtag cattaattct cggtcttgct
     1501 ggcggcgtca gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgccgtc
     1561 gccattggcg ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct
     1621 gccggaacca tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa
     1681 accttaaatg gcagtagtga agttccgcta tgggatcgtc tttttgcgcc gttaatggcg
     1741 gcagcagctg gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt
     1801 gcgcattacg gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt
     1861 gccatcgcag cagggatggt cgccgtatgg tgcttaccac ggttgcacgc gatgatgaat
     1921 caaatgaaaa atccggtgct cgtgctgggt attggcggat ttattctcgg aattctgggg
     1981 gttattggtg gaccagtttc gctgtttaaa gggctggatg agatgcagca gatggtggca
     2041 aatcaggctt tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg
     2101 gtcgttgctg ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc
     2161 gtggcattag ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt
     2221 tcctgcgcta ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt
     2281 atggcggcag tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt
     2341 ccggcatggc tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag
     2401 ccaccccacg ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga
     2461 tgatacgggg ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg
     2521 tgaaggagaa taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc
     2581 ccgcttgcag caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc
     2641 gatcgcgatc attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg
     2701 catcgcaatt atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt
     2761 ggctgggata agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac
     2821 gatcatcgcg gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag
     2881 cacaatgcct gatgcgacgc tagcgcgtct tatcaggcct acaaaccggc tctttaacgc
     2941 gtagggcgga taaggcgttt acgccgcatc cgccaatagt gccagatgcg acgctcattc
     3001 aactacaatc ccagcgccgt ccccaccaac aaccagatat tcagcgccac gaccagcact
     3061 acaatcaccc agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc catcaacttg
     3121 ctgtcactgg tgaaaatcag cagtggaacc agcgccaggg cgataccaaa acttaacagc
     3181 acctgactca taaccagaat ccgtgtcgga tctaatccca tcagaatgac aataaatgac
     3241 ggcaacatgg tgactgtacg acgcacccac agcg
//
LOCUS       DAZXUK010000339         3231 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZXUK010000339 DAZXUK010000000
VERSION     DAZXUK010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.341"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..203
                     /locus_tag="ACH3L8_003943"
     CDS             <1..203
                     /locus_tag="ACH3L8_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086834.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="ACH3L8_003944"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="ACH3L8_003944"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFT4086835.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2102..2818
                     /locus_tag="ACH3L8_003945"
     CDS             2102..2818
                     /locus_tag="ACH3L8_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFT4086836.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFHNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          911 a          699 c          698 g          923 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaacaacat tagtttttat aaaattaaat ataagattat
     1981 ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat ataatatgcc
     2041 gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg agatcattcc
     2101 agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc
     2161 taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gccaaacaag gtgaacccga
     2221 tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg cacaagttcc
     2281 aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg aaacgttcgt
     2341 acagggacgt tatgaaggct ttggtcctaa tggctcaatg attattgctg agacattgac
     2401 ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa
     2461 tatcggagcg gcaggttctg tcagctatat gtttcacaat acgggtgtga ttgtatttaa
     2521 aggaacagac cctgaccata tttttgaaat tttgcttgaa gctgaagttg atgttcgtga
     2581 tgtaactgaa gaagaaggta atattgttat ttatactgaa cctgctgacc ttcataaagg
     2641 aatcgcggcg ctaaaagcag ctggaatcac tgagttctca acaacagaat tagaaatgat
     2701 tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag ggcttgttga
     2761 tgcccttgaa gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa atctctaatt
     2821 atattttaaa gaaatctgtc tttatggcag atttctttaa tctcatataa ttcttataaa
     2881 aaatataata tttaactcgt catattgctt ataaaaaatc cccgtcccca gagaaataat
     2941 atttattaaa atggcaggtc ttctttttct gattaccgaa gacaaagtgg caattacgta
     3001 tagtttcccg ataaagacgc gacagctacg tcccgcataa ggcatttttc tctttatctt
     3061 tgtacggtac ttcatggaac agaatttttg accttgtgaa tcgtgatatc tgttggggag
     3121 gggcaatttg ctcactgaag cgtgagactc gattaagcgc acgaaacaca gaaatcaaaa
     3181 aacccggcca cttttttaca aagtggccgg gtaaaaataa tttttatttt t
//
LOCUS       DAZXUK010000340         3205 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZXUK010000340 DAZXUK010000000
VERSION     DAZXUK010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.342"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(61..588)
                     /gene="mobB"
                     /locus_tag="ACH3L8_003946"
     CDS             complement(61..588)
                     /gene="mobB"
                     /locus_tag="ACH3L8_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFT4086837.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(570..1154)
                     /gene="mobA"
                     /locus_tag="ACH3L8_003947"
     CDS             complement(570..1154)
                     /gene="mobA"
                     /locus_tag="ACH3L8_003947"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFT4086838.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1224..1493
                     /locus_tag="ACH3L8_003948"
     CDS             1224..1493
                     /locus_tag="ACH3L8_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFT4086839.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1570..2556
                     /gene="srkA"
                     /locus_tag="ACH3L8_003949"
     CDS             1570..2556
                     /gene="srkA"
                     /locus_tag="ACH3L8_003949"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFT4086840.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2573..3199
                     /gene="dsbA"
                     /locus_tag="ACH3L8_003950"
     CDS             2573..3199
                     /gene="dsbA"
                     /locus_tag="ACH3L8_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFT4086841.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
BASE COUNT          814 a          770 c          783 g          838 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc gggtgcgacg ctggctgcgt ctgatccagc ctactcttgc
       61 ttatccgttc tgcttttgca tccactccac cacaaaatca gccagcccct caacatcatt
      121 aatatccagt aatgcgacat caagattaag cggcacatca ctggctacag caataacatg
      181 cctgtctatc actaattctt caggtcgatg tccggctcca tcgcgaaaca gcacaatctt
      241 tgcgatctct tcatgcttaa acccttcgac cagaatcaaa tccagctttg aggtatccat
      301 tcgacttgcg agaaaatgta gatccagctc ttcttcgtct ggtgtttccg tcatcaaggc
      361 ccatcgttgc tggctggcaa cgatggtttg taccgcgcca gccttgcgca gctcatagct
      421 atctttgcct ggcttatcaa catccatatc atggtgcgta tgcttaatca gccccggacg
      481 gatccctctg gcgcataatg ccgggatcag ttttttcaaa agcgtagttt ttccggtacc
      541 actccacgcg gcaaaggcga gtaacggtat catcgttttt cctgccatcg ggcaagctcc
      601 tctggcgtat tcacgttaac aaatgcatct ttatgatcgc tgaaatcaac cgcatgaccg
      661 ccagccagac gcataaatgc cattacccgg cgttctcctg cttgcagata ttccagtaat
      721 aaaggctcaa tagcgcggtt taccagagca atagtcgggt gatcgcgttc accgtcatgg
      781 acccacacga caggcgcatc tttgcgctga tgattaagcc gggcggctaa atcatgggga
      841 atgtaaggcg tatcgcacgg acaaaacaaa aaccactcac cagcttcctg ctgcattact
      901 gaaagcattc ctgccagagg gcctgggtaa tccgccagtg aatcttcaat cactttcaga
      961 ccgcctgcct gatagatttc ctgatgacga ttagcattaa ccacgacgtg agagagctgc
     1021 gtcttaagcg cgtcagcgac atgttgccac agcggtttgc catttaagtc cagcaatcct
     1081 ttatctacgc cgcccattcg tctggcttta ccgcctgcca gcacaacgcc tgttatcgtc
     1141 gtcattagat tcaccgatat cgcctctttt attgtgggat tgaccctgct aacgtgtctg
     1201 tctcaagagt aaggagcatt actatgaaat gtaaacgtct gaatgaagtt attgaactcc
     1261 tccagccagc ctggcaaaaa gagccagact ttaacttgtt gcaatttttg cagaaactgg
     1321 cgaaagagtc aggttttgac ggcgaactgg cggatttgac ggatgacatt ctgatctatc
     1381 acctgaaaat gcgtgattcc gcgaaagatg cggtgatccc gggtttgcag aaagattatg
     1441 aagaagactt caaaacggcg ctgttacgcg ctcgcggcgt aattaaagag taaaagcttg
     1501 taagcggcgc caccaaaatc atcgtgaaat gatatccttc atcattcgta atgttttccg
     1561 gatgatggga tgaacaacag cgcttttact ttccagacac tacacccgga taccatcatg
     1621 gacgctctgt ttaagcaagg gatccgggtg gattccggtc ttaccccgct taacagctat
     1681 gaaaaccgtg tctatcaatt tcaggacgaa gagcgtcgac gttttgtcgt caaattttat
     1741 cgccctgaac gttggacagc cgatcaaatc ctcgaagaac atcaatttgc gttgcagctg
     1801 gtaaatgatg aagttccggt cgcagcacct gtggccttta acggtcagac tttattgaat
     1861 catcagggat tttatttcgc tgtttttcca agcgtcggtg gtcgccagtt cgaagctgat
     1921 aatatcgatc agatggaagc ggttgggcgt tatttagggc gtatgcacca gacggggcgc
     1981 aaacagcttt ttattcatcg cccgaccatc ggtctgaatg aatatctcat tgagcctcgc
     2041 aagctgtttg aggacgctac actgatacct tccgggttga aagcggcatt tctgaaagcg
     2101 acagatgagc tgatcgccgc cgttacagca cactggcggg aagatttcac cgttctgcgg
     2161 ctacatggag actgccacgc cgggaatatt ctctggcgcg atggtccaat gtttgttgat
     2221 ctggatgatg cacgtaatgg tccagctatt caggatttgt ggatgttgct caatggcgat
     2281 aaagcccagc agcggatgca actggaaact attattgaag cttatgaaga atttagcgag
     2341 ttcgacaccg ctgaaatcgg actgattgaa cctttacgcg ccatgcgttt ggtttattat
     2401 cttgcctggc taatgcggca ttgggctgat cccgcgttcc cgaaaaattt cccgtggtta
     2461 accggggaag attactggct acgacagacg gcgactttta tagaacaggc aaaagttcta
     2521 caagaacccc ctttgcaatt aacacctatg tattaatcgg agagagtaga tcatgaaaaa
     2581 gatttggctg gcgctggctg gtttagtttt agcgtttagc gcatcggcgg cgcagtatga
     2641 agatggtaaa cagtacacta ccctggaaaa accggtagct ggcgcgccgc aagtgctgga
     2701 gtttttctct ttcttctgcc cgcactgcta tcagtttgaa gaagttctgc atatttctga
     2761 taatgtgaag aaaaaactgc cggaaggcgt gaagatgact aaataccacg tcaacttcat
     2821 gggtggtgac ctgggcaaag atctgactca ggcatgggct gtggcgatgg cgctgggcgt
     2881 ggaagacaaa gtgactgttc cgctgtttga aggcgtacag aaaacccaga ccattcgttc
     2941 agcatctgat atccgcgatg tatttatcaa tgcaggtatt aaaggtgaag agtacgacgc
     3001 ggcgtggaac agcttcgtgg tgaaatctct ggtcgctcag caggaaaaag ccgcagctga
     3061 tgtgcaattg cgtggtgttc cggcgatgtt tgttaacggt aaatatcagc tgaatccgca
     3121 gggtatggat accagcaata tggatgtttt tgttcagcag tatgctgata cggtgaaata
     3181 tctgtccgag gaaaaataat tcaat
//
LOCUS       DAZXUK010000341         3187 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZXUK010000341 DAZXUK010000000
VERSION     DAZXUK010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.343"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            168..1271
                     /gene="proB"
                     /locus_tag="ACH3L8_003951"
     CDS             168..1271
                     /gene="proB"
                     /locus_tag="ACH3L8_003951"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFT4086842.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1283..2536
                     /gene="proA"
                     /locus_tag="ACH3L8_003952"
     CDS             1283..2536
                     /gene="proA"
                     /locus_tag="ACH3L8_003952"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFT4086843.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2651..2726
                     /locus_tag="ACH3L8_003953"
     tRNA            2651..2726
                     /locus_tag="ACH3L8_003953"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2684..2686,aa:Thr,seq:cgt)
     gene            complement(2741..>3187)
                     /locus_tag="ACH3L8_003954"
     CDS             complement(2741..>3187)
                     /locus_tag="ACH3L8_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4086844.1"
                     /translation="ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
BASE COUNT          752 a          807 c          877 g          751 t
ORIGIN      
        1 tggtaattta ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc
       61 gcgcaacaaa acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa
      121 aacgttcgtt tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc
      181 agacgctggt ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc
      241 gtgcccatat cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga
      301 ttgttattgt gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac
      361 tgccagcgac tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc
      421 aactgtggga acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc
      481 gtgctgatat ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc
      541 tcgataacaa tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta
      601 aggtcggcga taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac
      661 tgttgctgct gaccgatcaa aaaggtttgt ataccgctga tccgcgcaga aatccgcagg
      721 cagaactgat taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca
      781 gcgtttcagg cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt
      841 gccgtgcggg tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg
      901 tgatggaagg catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc
      961 gtaaacgctg gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa
     1021 ctgccgccat tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg
     1081 gcaatttctc gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc
     1141 acggtgtcag tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag
     1201 aaattgatgc aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga
     1261 ttacccgtta aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc
     1321 gtataaatta gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga
     1381 tgaactggaa gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc
     1441 gcgagccaat ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct
     1501 gaaaggcatt gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt
     1561 aatcgatggc ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg
     1621 ggttattggc gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg
     1681 cctgaaaacc ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc
     1741 ggcaacggtg gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt
     1801 gcaggcgatt gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata
     1861 catcgacatg ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc
     1921 gacaatcccg gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagtgc
     1981 agagatcgct gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg
     2041 taatacggtt gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt
     2101 aagcaaacaa atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca
     2161 gttgcaggca ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt
     2221 tctgtcatta gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat
     2281 tcgtgaacac ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca
     2341 gcgttttgtt aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac
     2401 cgacggcggc cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc
     2461 gcgtggccca atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta
     2521 caccattcgt gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc
     2581 attgacgcat cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg
     2641 tcctttcagg gccgatatag ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt
     2701 aggttcgact cctattatcg gcaccattta aatcaataag ttacacatca ttagtacctt
     2761 ccttatttct tgactgggac aaatttggga ccgatgggtt cagaatcaag tctatttgcc
     2821 gtgcgtgttc ggtaaggtga ttaggtgcga ggtgagcata tcgacgaacc atttcgatag
     2881 actcccagcc tcccatttcc tgtaacactg ataacgggac tccggcttga accagccaac
     2941 ttgcccaggt gtgtctcaag tcgtgaaatc tgaaatcatc aataccagcc cgtctcagcg
     3001 ccgctttcca ggctgtgttt gcgtcatacc gcatcttcct tactgttggc gctttcgttc
     3061 cgtctggttt ggtacagctt tccttgtaca caaataccca acggtgatga tttccgattt
     3121 gttttttcaa tacgcgacat gcagtatcat tcagcgcaac gccaattgcg cggtttgatt
     3181 tactctc
//
LOCUS       DAZXUK010000342         3186 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZXUK010000342 DAZXUK010000000
VERSION     DAZXUK010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.344"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(91..1308)
                     /locus_tag="ACH3L8_003955"
     CDS             complement(91..1308)
                     /locus_tag="ACH3L8_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4086845.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(1675..2661)
                     /gene="ycdU"
                     /locus_tag="ACH3L8_003956"
     CDS             complement(1675..2661)
                     /gene="ycdU"
                     /locus_tag="ACH3L8_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFT4086846.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(2658..3186)
                     /locus_tag="ACH3L8_003957"
                     /pseudo
     CDS             complement(2658..3186)
                     /locus_tag="ACH3L8_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT          931 a          805 c          633 g          817 t
ORIGIN      
        1 caggttttca ttacgaactg gcattgcatt tatttttcaa aaaagaaagc agtaaccatt
       61 tggctactct gagtatgcca gtccatgaaa ctaagcgaat tccttagtgt ttttagcaag
      121 ttgagacatc acataatccg cccatgcctg catcatcggt acacgctgct ccagtagatc
      181 agtacgatga tatgccgcct caaccttatt tttcagcgta tgagcgagcg ccctttccgc
      241 cagatcccgc gaatacccct gttcactaca ccagtccctg aatgttgagc gaaaaccatg
      301 tgccgtggca actcgccccg gaatgtcact gacggctttc tttttacgca gaaaacttgt
      361 caacaccata tcggaaagga tctgctgctt tctgggtgaa gggaacacca gttcatcatg
      421 caggccacgt atattttcca gaatgtaaat agcctgccgg gataaaggaa cacgatgctg
      481 tagcctagct ttcattcttt ctgcaggtat agtccatacc cgcttatgaa aatcaatttc
      541 agcccagcgc attcccctgg cttcgcccga gcgagttgct gtaagtatca ccattaataa
      601 cagtgcgcgg gtaacattat aaggttcatc actatacaca ctggtcgcca caaaaagcgg
      661 taactgcctc cagggcattg cgggttggtg ttcatcacgt cctcttgtct gctgaggaag
      721 caaatggtca accacatcaa caggatttgc cacacaaaaa ccgtgcgccc atccccactg
      781 cataacaaca tgaatgcgct gtttaacccg gcttgccgtt tctgacaagg ttaaccagac
      841 tggacgcagt gtttctgcca cgtccgcagc cgtaatcgaa tccagcgttt ttgctcccag
      901 ttgaggaaac gcgtaattct caagcgtcga taaccactgc cttacatgct ttggattctc
      961 ccatccagga gacagttctg catgtacacg cctggctgca tcggcaaatg ttgggataac
     1021 gactttctca gattcagcct ttttaatctc cagaggatca tcacctgcag caagttgctc
     1081 tcgcattatc cgggcagtac gtgcagcttc agcaatactg acctctgggt aagttcccaa
     1141 tccagcatta cgtctttttt gtgtcaccgg acttacataa cgaaaaaccc atttcccccg
     1201 cccctttact gaagaaggat gaagggtcag tccggtaatt cccccatggg gcaatggttt
     1261 gtcatcaggt ttgatatgtc ttgctttcgt atccgtcaat actgccatac gctaattccc
     1321 gtcactctgg tatgccatcc agtatgccat tagtccctcg cttcactaag aatacattag
     1381 ataacatcgg acaacaaaaa gtgtaataca ttgtttataa agaaattata taacacccct
     1441 ggattaagtc agatttattt caggcggtcc ccctcaccgc catatttaaa gaagagcgcg
     1501 tacgaaagta cgggcttttt tttcgtatat tgtacacacc gggtgccccc gcccacgtct
     1561 gttagggcga ggggaaattg tgcacatcaa ctgtctggtt actcagtcaa caatcaaaat
     1621 gcgtaagata gtacagaaca tatatgcgtt atctggtcac ttcctgtaac ttcctcactt
     1681 attaaaatgc ccccaacgga aatacatgtc acatgccatc ataaaatcgt tatgccagag
     1741 gaatcgacaa tggaaaaaat agtaaattaa gcacggtaca tagcagagta aaaaataggc
     1801 ttcaaaccaa atccgccatt ccacaagcgt tgccaatacc gacaccagcg taatccacat
     1861 catttcaata acccgcaata tgaataaccg aagccaaagc ggcttggcaa aaaaggtcga
     1921 caacgtattt tcactggaga aaaacagatc gatttcctgc aaaaaaaacc tgcccagaaa
     1981 agcgggccgc ttggagaagc gcagaaaaaa cagattaatc gccaacacta cgccacaaag
     2041 aatgagcaat gcaattatcg atttaaacgt taaccactgc ccctctgtca caaaagaatc
     2101 actgttacgc agcgggctga tagtaagcag gttaacaacc gttgagatgc acaccgaaag
     2161 cagcacatag cgagtcagac ttttatattt tacttttttc gctactgaaa tagcgacctg
     2221 cttgacataa ctggtgcgga tcatccgaaa cagcagaaag atgaccaacg gcccaagtaa
     2281 cgaaatcagg aacagcgcat ataacgaggg tagaagctgg tatagccccc aactcattgc
     2341 actcaacatg attaacgaca gaaatcctgc caccagaggt tgtgtaagaa ataattctat
     2401 tttgtggttc tgctttttct taaaccacga aaaatcgata ttcttcgtga cgcatttata
     2461 agcccagtac gccttgagat caaaaataaa attatacagc accagggcca gaatcccact
     2521 aactacacca atatcctctg atggaaaagt aaaccctgca tttttccaat aaaagcctat
     2581 cagaataagt ccaatagcat aaatgtatat cagatttcgt ttatcctgaa agcgaataaa
     2641 agttaaataa tcaggaatca tttgaatacc tttgcatcac taaatgcttt acgacaatgc
     2701 ataactcgtt gctgatccac gccagcaaaa tcatttaaca tattccccaa atagggctta
     2761 ttacttattt caaggaaagc taccggggtt ggcatattca gaatatgtat cgagagattc
     2821 agtccatttc accgtatgag ttaagtgtaa tgagagattt tctttgataa ttgacggagc
     2881 gacctcgctt tttgctgtta cgttcatcag aacctgatgc tctggcgaag cgatatccag
     2941 tccggcaaga taatcgcgca ttgcctgaac gccgtcttcc atcaaacgcg tgtgccaggc
     3001 accgctcaca ccgagtttaa ccggttcgta tccagcggcc atcagcagcg tggtaaattc
     3061 attcaacgca gcctgcgttc ccccaataac ctgctggcgc ggcgtgttat cacagctaat
     3121 atccagcgca atgcctgatt ccgtaatcat cgtctgcagt tgttcgcggt tgatgccttt
     3181 aaccgc
//
LOCUS       DAZXUK010000343         3180 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZXUK010000343 DAZXUK010000000
VERSION     DAZXUK010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.345"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACH3L8_003958"
     CDS             418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACH3L8_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFT4086847.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(1772..1906)
                     /locus_tag="ACH3L8_003959"
     CDS             complement(1772..1906)
                     /locus_tag="ACH3L8_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFT4086848.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2552..2722)
                     /locus_tag="ACH3L8_003960"
     CDS             complement(2552..2722)
                     /locus_tag="ACH3L8_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFT4086849.1"
                     /translation="MNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSI
                     QIRFEAESETGT"
BASE COUNT          800 a          659 c          776 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg ggttttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg gggggggacc ctgagcaggt ggtaattgta agacgttgcg
     2821 ggcgacggta ttacaattgc acatcctgtc ctgtttcttt aggacgtatc atggcacagc
     2881 atatcaactg ataaataatc cctgtgcagt gcagccgctg ttgcataaaa tttcgctctg
     2941 cgtcgagtgt ttcttaatca ataattccgc tgatactccg gaaaaacgcg agtctgttct
     3001 catgcctgaa atgcccacac cccacgcaaa aacaagtttt tgctgatttt tctttataaa
     3061 tagagagtta tgacaaatta gttcttcttg ctctctttgt gatatttaaa aaagcggtgt
     3121 cggcgcggtg ttgtagctgc gccaacaccg ctttttaggg gtggtactga ctattttcat
//
LOCUS       DAZXUK010000344         3178 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZXUK010000344 DAZXUK010000000
VERSION     DAZXUK010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.346"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(145..264)
                     /locus_tag="ACH3L8_003961"
                     /pseudo
     CDS             complement(145..264)
                     /locus_tag="ACH3L8_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            508..880
                     /locus_tag="ACH3L8_003962"
                     /pseudo
     CDS             508..880
                     /locus_tag="ACH3L8_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1442..2389)
                     /gene="icsP"
                     /locus_tag="ACH3L8_003963"
     CDS             complement(1442..2389)
                     /gene="icsP"
                     /locus_tag="ACH3L8_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFT4086850.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
BASE COUNT          934 a          679 c          613 g          952 t
ORIGIN      
        1 caaaaatgca attatattct ggcattgttt aatgcggttt gtctttttta ttcattattt
       61 caatgaaaga atttcaacta caaagttaat tgatcctacc tacataatat agacacagta
      121 cctaaagtac cgtggagatc atccggggaa gccccgggtc tgataccagt gcaggtagac
      181 cgctatcttg ttccggtaat gttctgccgt tctttacggc atcacctcta gcagaaacat
      241 cgcgtaactg cgccaagtat gatgaacttc tcttaccgct gcgatctggt tcaacaatat
      301 gcagatggta cacaacctct ttaaccagcc catccggatt attcagagcc gcttccgcaa
      361 tccggaataa aagtgctgtt ttaccgtgaa ccctctgaaa atcagcgagc agtgttttaa
      421 ccagccgacg ttcagagcgt actgacagac ggtggacaat ttgaataaac agttcaatca
      481 gaccgtagat gctctcccgt tggcgacatg ctgggagcgg tggaacaccg cttcggcaac
      541 gagcttccgg cgtctccagt gaagtgactg acggataatg gttcatgcta ccgagctaat
      601 gaaacacgcc agttcacccg gatgttggga ctttgttgaa ccgaagaaca cggcggtgcg
      661 gagtccggag agtaacagaa tagcaaagag cttcgtgaaa acaataaagt gtgactacat
      721 cagtatcatg cccaaacctg acgggttaac ggcagcaaag aaccttgcag aggcattcga
      781 gcattataac gaatggcatc cgcatagtgc gctggattat cgctcgccac gggaatatct
      841 gcggcagcgg gccaatgata acaggtgtct ggaaatatag gggcaaagcc acccacgaac
      901 gatccagcat ataatcaaga aattagattt catttaacat aagatagatt atgtacacca
      961 agaggcgttt ccttaatttc tgaatttaca ggctcttttg ctctctttgc tatgctgtgg
     1021 atttggaaag atagagtgct ggctgattat ttaggtattt tcatcagcat gtccctgata
     1081 gcactgttcc atcagcgctt tcaggcgata atagtagacc ttgggtagcc tctgcccatg
     1141 tgtaacttga taccccgaga acagccacga tgatctaagc ggttcttatt tctccggtct
     1201 cagggtcggt aatgccaaca gtaagtccac agtaatcaac gaagagtttt ttgaaccgcc
     1261 ccggaaatcc tggagactaa acgccctgag aaagaggtaa acagaatgac taaaaataca
     1321 cgtttttccc ccgaagtccg tcaacgggca gttcgtatgg ttctggaaag tcagggcgaa
     1381 tatgactcac aatgggcggc aatttttagc catgaaaacg taatcaactc ggtgaaccaa
     1441 ttcaaaaaat atactttata cctgcggaag tgagaaaacc aatatattca atacttgcac
     1501 tatttttaat ggtgccagat atattggtac tttgctcata aagagatgta tcaccttttt
     1561 tattagaaat gtaatttctg gagccttcta tataaaattt tgcactgggg gtgacatagt
     1621 atccaatatt cgcagcaaca gagcagtaat tttggttttt tatttcatct ttaaaaattg
     1681 tctcagtctg atagtgttta tctgtatcag atgaaagaac ccaaccacta tatttcagtt
     1741 ctgctccaaa ctcaaaattc tcatggcggt aattagcagt taatccaata taaggtattt
     1801 tgaagagctg tttgtaacct attgttcttt caccactagg atgtgcccct tttttattcc
     1861 tgctcccccc gttttcacta taaatataac tccctcccat tgcattaaaa ctgtaacgac
     1921 tctcttggta acctgctatt agtccaagtc gataatccaa attatttaat aaccaacctt
     1981 taagattcag ctcgaattcg ttagcatcac ggagatgcgt attgggatgc cagctctggt
     2041 cggtccatac ctgaggggta ttggaattgt tccagtcttt atcaaccatg cttgctttct
     2101 ggttacctaa agtagtccaa ccggaaactc cgaaagacac ttttggaatt agcttccaat
     2161 cgatgccacc tctaacaata gtcgcattac tgtatttcca gtccagttga ctaatttttc
     2221 tccccccttc cttgggatga taaacgcgtt cttttgtttt gccactcaga gatcctagac
     2281 taatatccgt actgatgttg tccggtatga aaagtggata gttagtggta gcatgtgtag
     2341 taaagatcgc aggtacacaa agtgcaagta caaagaattt taatttcatc gagaactcta
     2401 cttttttggt tgaaatgtcc atgatcttct tactttataa gataaacgtc aatgccaagc
     2461 attattttaa ttaaatattt gtttatgtta ccaagaacat atcttttatt ataatattta
     2521 ttgaactttt gtaaaaataa aaaaatacct cattgtgcga ataaagtaac gggggccata
     2581 gatttctcta cctacctttt ggtgttcata cctgaggctg atagtttcag gattccgcga
     2641 caattctact caccttcttg ttctctcgcc aatatgaaat caatcagaaa agcgttccat
     2701 aagaacaacg ataagacaat aaattgtcat cacggctgca atagccaacg tgcctttcag
     2761 taccaacatg acaacctcct gtatttgacg taacgtgtgc tgataaatat gtatttgtat
     2821 ctcaactgac tcaatacaac acttaagtat agtttttcag attttgtttt tcccgtgaaa
     2881 tattatggaa atggcagaag caatacattg aattccgatt cgagtgaata cggctagcgg
     2941 gcacctcact ttagcactga agccatcaac ttttatttga cttatttcaa tggaaagctt
     3001 tctcactatc agtacaggta attcccatcc ttttaaagtc gagagtcaga ctgcgactaa
     3061 tacagttaaa gtgaccaaac tcagttatcc tctacacatt ttatctcatg tttacttgga
     3121 caattcattg gtgcttttat aaaagaataa atattctact aatttaatgc atcaccta
//
LOCUS       DAZXUK010000345         3167 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZXUK010000345 DAZXUK010000000
VERSION     DAZXUK010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.347"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            15..1055
                     /gene="gatD"
                     /locus_tag="ACH3L8_003964"
     CDS             15..1055
                     /gene="gatD"
                     /locus_tag="ACH3L8_003964"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFT4086851.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
     gene            1161..1934
                     /locus_tag="ACH3L8_003965"
     CDS             1161..1934
                     /locus_tag="ACH3L8_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFT4086852.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2016..2915)
                     /gene="yegS"
                     /locus_tag="ACH3L8_003966"
     CDS             complement(2016..2915)
                     /gene="yegS"
                     /locus_tag="ACH3L8_003966"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFT4086853.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT          840 a          706 c          798 g          823 t
ORIGIN      
        1 aatcaggagt ttttatgaaa tcagtagtga atgatactga tggtatcgtg cgcgttgcaa
       61 aaagcgtcat tcctgaaatt aaacatcagg atgaggtgcg ggtaaaaatt gccagctcgg
      121 gcttatgtgg ttccgattta cccaggatat ttaaaaatgg tgcacattat tatccaataa
      181 cgttaggcca tgaatttagc ggctatattg atgcggtggg atccggtgtt gatgatttac
      241 accctggcga tgcggttgcc tgtgtgccgt tattaccctg ttttacttgt ccagagtgtt
      301 tgaaagggtt ttattcccag tgcgcaaaat atgattttat tggctcacgg cgtgatggtg
      361 gatttgctga atatattgtc gttaagcgaa aaaatgtctt tgctctaccc acggatatgc
      421 ctattgagga tggggctttt attgagccga ttaccgttgg cctgcatgct tttcatttag
      481 cgcaaggttg tgagaataaa aacgttatta ttattggtgc cggaaccatt ggcctgctgg
      541 ctattcagtg cgctgtcgcg ctgggagcaa agagtgtgac ggcgatcgac attagctcag
      601 aaaaactggc actggcaaaa tctttcggtg cgatgcaaac atttaacagt agcgaaatga
      661 gcgcgccgca aatgcagagc gttttacgcg aactgcgctt taatcagctt atcctcgaga
      721 cggctggcgt accgcaaact gtcgaactgg cggtagagat tgccggtcct catgcccaac
      781 tggcgctggt gggcacgttg catcaggatc tgcatttaac atcggcaacg tttggcaaaa
      841 tattgcgtaa agagctgacg gttatcggca gttggatgaa ctattccagc ccttggccgg
      901 ggcaggagtg ggaaacggcg agccggttgc tgacagaacg taagttaagc ctggagccat
      961 taatcgctca ccgtggaagc tttgaaagct tcacccaggc ggtgcgtgac atcgctcgta
     1021 atgccatgca gggcaaagtg ttgctcattc catgaaaccg cgggccagcg tgatgctggc
     1081 ccggtattgt gcaaaacaga tcattcacca atggtccccc ttcgtttaca ctagccacaa
     1141 ttgaatatgg gtaaatgacg atgaattcat tcgagcgaag gaataagatc atccaattag
     1201 tgaatgaaca gggaaccgtg cttgttcagg atctggcggg agtatttgct gcctcggaag
     1261 cgacaatccg tgccgatttg cgctttctcg aacaaaaagg cgtggttacg cgctttcatg
     1321 gcggtgcggc gaaaataatg tccggtaata gtgaaaccga gacccaggaa gtcgggttta
     1381 aagagcgatt tcagctcgcc agcgcgccaa aaaacagaat agcgcaggcg gcagtcaaaa
     1441 tgatccatga agggatgacc gttattctcg acagcggaag taccacaatg cttatcgccg
     1501 aaggattaat gaccgctaaa aatatcacgg tgattaccaa cagtctcccg gcagcgtttg
     1561 ccctttccga aaataaagac attactctgg tcgtctgtgg tggcacggtc cgccataaaa
     1621 cgcgctcgat gcatggttcc attgccgagc gttcattgca ggatattaat gccgatttaa
     1681 tgtttgtcgg tgctgatggt attgatgcgg ttaatggtat tacgaccttt aatgaaggtt
     1741 attcgattag cggggcgatg gtcaccgccg caaataaagt gattgccgtt ctcgattcat
     1801 cgaaatttaa tcgtcgcggt tttaatcagg tactgccaat cgaaaaaatt gacattattg
     1861 ttaccgatga tgctgtgtca gaggtggata aattggcatt gcagaagaca cgggtaaaat
     1921 taattacggt gtagtattgt tctgtatgcc ggataaggtg tttacaccgc atccggcatt
     1981 acggattctc cctggttcaa gagagaatag tttgattaac gcaataatgg acaatctggt
     2041 ggtaatcgac aacgcaacgc cgccggaagt atttcaatat ggaagttttg cccactcaac
     2101 ggttcgccat caagattaaa agtgatttcg tgtggggctt gtatatcaaa ccacgacgaa
     2161 gcgccttcga taatattcgg gttatcttcg tcagatttta aggttgatac gagagccgga
     2221 agaatttcat cgccggtaaa aatgcgcagt tgcagcaagc catcgttaat taacgcgttc
     2281 gggcacaatt gttgaccgcc accggcctga cgcccgttac caataccaat gaccagggcg
     2341 tcaccttgcc agtgaaagtt ttcaccgcgg atttcacaac ggtccggttg cagagtatcc
     2401 atgcgcatta agccatgaat gatgtaagag acgccaccca gcgcggcttt taatttttcc
     2461 ggcgtttctg tggtaatacg cgtcccaaat ccgcctgtcg ccatattaat aaaacaggtt
     2521 tgtttgttga cctgcgccat atctatcgca atggcattac cggcaattgc cagtttcagc
     2581 gccttatcca gtgcctcagg aatccctaca ctggtggcaa aatcattggc ggttcctaat
     2641 ggcaaaattc ccagcgcggg tatgtcatcc ccctcacact gaatcaacgc cgtagaaact
     2701 tcattaatgg tgccatcgcc accaccggca atcaccgttg cgacgcccaa cttccgggcc
     2761 tcctctacaa atcgtgcggc atcacctttc tcccaggtga cccgcacatg gatcgtcatt
     2821 ccttcctcac gcaacagcat aattgcttcg cgcaagggta gattgtcagt acttttacca
     2881 ttaagaatca gtaagctggc gggaaattct gccatgatcg tatgtgcctt tatgattggt
     2941 ctgtagatag tgtagagcag aaaacaaaag gtggagtcag aacaggatga aagtcggagg
     3001 ataaaaagtc agttcaagta tggaggattc agtggttacc accaggagct tgcaatgggg
     3061 acagcgaata ctaacggctg ccaccgataa atttcaaaaa agagcatata cctaatgttc
     3121 aactaaacag tggcatcttc aatataatat attaaagccc ccatgga
//
LOCUS       DAZXUK010000346         3160 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZXUK010000346 DAZXUK010000000
VERSION     DAZXUK010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.348"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(216..839)
                     /locus_tag="ACH3L8_003967"
     CDS             complement(216..839)
                     /locus_tag="ACH3L8_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFT4086854.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1136..1411
                     /gene="ypdI"
                     /locus_tag="ACH3L8_003968"
     CDS             1136..1411
                     /gene="ypdI"
                     /locus_tag="ACH3L8_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFT4086855.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(1488..1730)
                     /locus_tag="ACH3L8_003969"
     CDS             complement(1488..1730)
                     /locus_tag="ACH3L8_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFT4086856.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            2083..3003
                     /gene="lpxP"
                     /locus_tag="ACH3L8_003970"
     CDS             2083..3003
                     /gene="lpxP"
                     /locus_tag="ACH3L8_003970"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFT4086857.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
BASE COUNT          864 a          735 c          669 g          892 t
ORIGIN      
        1 attacgcttt ttaaagcgta ccataaagca taacataata aatgatttgt gatgacacgg
       61 gattattgcg gtttactttg aggaaaataa aatgcgtgtt tatagaaccg cgagcgccaa
      121 ataattttat gaccacaacc tgtcgtattg tggtcataaa aaattgtcgg ctggagggag
      181 ttaaatcctt tacctcaata tactgcctgt cagcattagt tcacaaacag tgcttcgcta
      241 tcgacgatga ttccatcttc cgcaccttta agcgccaggt ttgcctcgta gtattgcttt
      301 ttatcaagca gtttctgcgc atcagcaagt gcattacgag tctgtttaag cggcatcaga
      361 tattggtttt ccattacgcc aacaccggcc aggcgaagct cttccattgc gcccttttta
      421 tcccctttcg ccattttttc attggcaatt tttatcgcgg cttctttttc gggtgttgct
      481 acataactct cagaaattcc gacagatgca ttaatgacaa tatattggtc atcattgaga
      541 ttggtctttt tacctggctt agcgaacttt gcccattcgg tactgtcatc tgacagcaaa
      601 gcggaagctt cattggtcag ttcttttgcc ttttccggat cgccatgaaa cagagccagg
      661 cgcgctacct gcacgtcacg catcgcatat aaaccttgtt cagagatacg ctctgctgct
      721 gccgttttct gggtttgctg agtttgctgt tgtgccgcta ccggtgcatt atcagcagcc
      781 catacagttg aagatgccag aattgctgtg accatcgtag ccataattaa acgtttcata
      841 cttacctcat tctttaacag ttttagaaaa cgccgccatc atattgggtg agcatgaaat
      901 gagaaatcag agagcaacgg tcattttgaa agggttatct tacaattgta gttattatcg
      961 caaagcggca tttagtctta cagttgtagg aatatgcctc ctgtcgcaga gaactattct
     1021 tatataccac aaagagatgt catttattat attgatgtaa atattgatta attatatgta
     1081 aatacagcca taacatcgta tggttctcag tgaaccattt tcaggataac tttctatgaa
     1141 agtaaactta atacttttca gcttattttt attggtctct attatggcat gcaatgtttt
     1201 tgcattttcc atttctggtg gtgtaagtga gaggagctat aaagagactg aaaaaacatc
     1261 agcgatgacg accacacact ctacaaaact tcagccatca caggcgattt tgtttaagat
     1321 gagagaagat gtgccaccat taaacctcac agaagaaatc acgcctactt atccgacaaa
     1381 ggcgaattat cttattcatc ctgtgcgata gcgtctgtca catggcaggt gaacaatcat
     1441 gtctgatgct gatgtgaaca cctgcccaaa tgagccatcc tgacccacta tcgcctgcca
     1501 taccccacca taaagcgaat catcaagcca caagccgggg caagaaacat aaatatcccc
     1561 gtcaccagtt cattaccgcc agttaaccac atggtgaagt aataaatgag tgcagcgagg
     1621 atcaccatgc cgaagaaaga agagaccgcc gataccacgt tcagtacctg accgatgtag
     1681 ccgcttacgc agacaataca cagggcgaga aacatccata aataaatcat agctcaacct
     1741 cctgccgcgc taaaaaacct gtaacttcag tcactttaat agtaagacaa tccttaacct
     1801 ctggcaattt ttgctaagca tcaatgaaaa cagcacgtta aatgcaacgc tattgcgtgc
     1861 ggaaaatttt ttactttgca cgattaataa tcagtagctg aaagcagtca gccagtgata
     1921 aagtagagcc accgcattca tgctcctcgt ggttatgtcc tgactagtct ttacactctt
     1981 tacaggaacc attgtcgtac atgatggccc aaccaattga gtgtaccgct gtcgacatgg
     2041 aatcttctgc gatacaacaa ttcgtatcta cagaaggtaa ctatgtttcc acaatgcaaa
     2101 ttttcccgcg agtttctaca tcctcgctac tggctcacat ggtttgggct tggtgtactc
     2161 tggctttggg tacagcttcc ttatcctgtt ctctgctttc tcggcacgcg tattggcgca
     2221 atggcgcgac cattcctgaa acgtcgtgaa tctatcgccc gtaaaaacct ggaactttgt
     2281 ttcccgcagc attctgcgga agaacgcgag aagatgattg ccgaaaactt tcgttcactc
     2341 ggcatggcgc tggtagaaac cggcatggca tggttctggc ccgacagtcg cgtacgtaaa
     2401 tggtttgatg ttgaagggtt ggataacctt aaacgcgcac aaatgcaaaa tcgcggcgta
     2461 atggttgtcg gcgtccattt tatgtcgctg gaactgggcg gccgcgtgat gggactgtgc
     2521 caaccaatga tggctaccta tcgtccacat aataatcagc tgatggaatg ggtgcagacc
     2581 cgtgggcgca tgcgctctaa caaagcgatg atcggcagaa ataatctgcg cggcattgtc
     2641 ggtgcactga agaaaggtga agcggtatgg tttgctcccg atcaggatta tggtcgtaaa
     2701 ggcagctcct tcgcgccgtt ctttgcggtg gaaaatgtcg ccacaaccaa tggcacctat
     2761 gttctctccc gtctctctgg cgcagccatg ttgaccgtaa cgatggtaag aaaagcggat
     2821 tacagcggat atcgtttgtt catcacccca gagatggaag gctacccgac agatgaaaat
     2881 caagccgctg cttatatgaa caagattatc gagaaagaga tcatgcgcgc accggagcag
     2941 tacctctgga tccaccgtcg ctttaaaacg cgcccggtgg gagaatcgtc gttgtacatt
     3001 taacaaactg ctcttactct ttccctcctc aaatcggcca taataacctc atgattttta
     3061 tagctttttt cttaagtttt ttccttcgct gaatatctaa ttattaccgt cagaaattaa
     3121 actgtcgccg atatgagaca cttgtaagtt actaaaagta
//
LOCUS       DAZXUK010000347         3124 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZXUK010000347 DAZXUK010000000
VERSION     DAZXUK010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.349"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            135..680
                     /locus_tag="ACH3L8_003971"
     CDS             135..680
                     /locus_tag="ACH3L8_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HFT4086858.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(664..1311)
                     /locus_tag="ACH3L8_003972"
     CDS             complement(664..1311)
                     /locus_tag="ACH3L8_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFT4086859.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            1523..2314
                     /locus_tag="ACH3L8_003973"
     CDS             1523..2314
                     /locus_tag="ACH3L8_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFT4086860.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            2324..>3124
                     /locus_tag="ACH3L8_003974"
     CDS             2324..>3124
                     /locus_tag="ACH3L8_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFT4086861.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINS"
BASE COUNT          790 a          782 c          774 g          778 t
ORIGIN      
        1 acaaatcccg gggaaaaaga aacaatagca atatcccgta tgaatataat aaaggcgggt
       61 ttaccacgga catcatagcg ttagcttatt cataggctat gattgaggaa caagacgtgg
      121 agcaggagga aaccatgccc gggcgctttg aactaaaacc aaccctggag aaagtctggc
      181 acgcgccgga caattttcgc ttcatggacc cgctgccgcc gatgcatcgt cgggggatca
      241 ttattgccgc cattgtattg gtggtcggat ttctgcttcc atctgatgat acgcccaacg
      301 cgtcggtggt gacgcgtgaa gcgcagttag atattcagtc gcaatcacaa ccgccgacag
      361 aagaacagcc gcaaactacc caaacgcaac cattccagcc agatagcgga attgataatc
      421 aatggcgctc ttaccgtgtc gaacccggta aaacgatggc gcagctgttc cgcgaccacg
      481 gactgcccgc taccgatgtc tatgcaatgg cgcaggtaga aggcgcgggt aagccgctga
      541 gtaatttgca aaatgggcag atggtgaaaa tccgccagaa cgccagcggc gtggtgacgg
      601 gcttaaccat tgatacgggg aataatcaac aggtcctgtt tacccgccag ccggatggca
      661 gttttattcg tgcgcggtag atgaagggtc aaccaaaagc cagctaagga ttgtctcttt
      721 gatgacctta cgtctggctt ccagtgcctg gggggaaacc atactgatat gataaactag
      781 gccaagcgta tgctggttga tcaaataata tcccccaagc gcagcaatat taatattcac
      841 ctgcaatggg tcgatacctg gcttaaaaat attgtatttc ttcccttcat ccaataagga
      901 ttccattaac tgcaagtgag catggttaat ttctaagaga cgctgtgatt ttgcataatg
      961 cacaccttta ctttgatttt cactatgcac aatttttaaa aaccaaggat tagcaatata
     1021 ataatcccat gtaaaatcaa taattttctc aatggctttt tgtggcggaa gatctgagaa
     1081 atcaagggcc ttttcttttt gtcgaatatc gttccaggta tattccagca cttcgataaa
     1141 taaattttct ttattaccaa agtggtgata aaccgtctgt ttactacacc ctgctgcctt
     1201 tacaatatta tctactctgg ccccttcata accatattcg gcaaattcat taaccgcaca
     1261 tagcagcaat ttttccttca ggctctgtac gttgctggta tcaatataca taacgcttcc
     1321 ccacgacctc ttcgtggtga ctatcataac ctgatttgat ttcagcttca gaaaaaaggg
     1381 caaaaacttg tactttcatc aggttatcaa atgtcctgtg atcgccatcg catatcaacc
     1441 aactagatgg tttatctttt tatcctccag tcgcaagcta atcatcacca aggaaacaaa
     1501 attcaaaaaa gagagctatt gtatgaaatc cactcgtcct gttgccgtga ttaccggagc
     1561 cactcgcggt attggaaagg gctgtgcgca ggagcttgct cgtggcgggt ttaacctgct
     1621 gattaacgat cgtcccgatg ccgacagcgt agaaaaactg catatcacac aacaagaatg
     1681 tttggcggaa ggcgtggaag tgatttgctt ccctgctgat gtcggtgatc tatctctgca
     1741 tgaggagatg ctcgacgcgg cgcaaaacca gtgggggcgg ctggactgtc tgctcaataa
     1801 cgcggggatt tcagtgaaaa aacgcggtga cctcctcgac ctcgaaccag acagcttcga
     1861 tcaaaacatt gccattaaca cccgcgcgcc gttcttcctt gctcaggcat tcagtaagcg
     1921 cttactggca cagccgaaac cggaagcgga gctgccgcac cgctcgatta ttttcgtcag
     1981 ttccatcaac gccatcatgc tggcgatgaa tcgcggcgaa tacaccattg ccaaaaccgc
     2041 cgtttctgcc gctgctcgtc tgtttgctgc tcgcctttgt aatgaacaaa ttggcgttta
     2101 tgaagtgcgc cctggtctca tcaaaactga tatgactatt ccggcaacgg cttattacga
     2161 cgaactgatt gccaaaggtc tggtgccgtg gggacgttgg ggttatccgg ctgatattgc
     2221 ttccaccgtc cgcgcgatgg cggaaggtaa actgatttac acctgtggtc aggcggtcgc
     2281 aatcgacggc ggcttaagta tgccgcgctt ctgaggctga atcatgagag atctgttaca
     2341 acgcccggat ttattcagta tcaataccgc gacactgggc tataaaacgc cgctacccgc
     2401 cattattgat gcctgcgccg cacgcggtat tggcgcaatt gccccctggc gcagggagtt
     2461 gcagggtgaa gatctccagc aaatcacccg ccagcttgcc gccagtaaca tgagcgtttc
     2521 cggtttgtgc cgtagcactt actacaccgc gccgacactg gcggaacgca aactggcgat
     2581 tgatgataac cgccgggcgc tggacgacgc agcggtgctt aacgccgcct gttatatgca
     2641 ggtggttggc ggtctgccaa cgggcactaa agatctatat gaagcgcgag aacaagtgaa
     2701 acaagggatt cgccagttgc tgccacattc gaaagatgtc ggcgtgccaa ttgcgctgga
     2761 gccactgcac ccgatgacag ctgccgatcg ctcttgcctg tgcacgttac gtcaggcgct
     2821 ggactggtgt gatgaactcg atcccgatgg tgagtttggc ctcggcgtgg cggtggatgt
     2881 ttatcacgtc tggtgggatc cggatctcgc cagtcagatc ctgcgtgccg gaaaacgcat
     2941 cctcgcgttt catgtttccg actggttaat gccgaccact gatctggtca atgaccgtgg
     3001 tatgccggga gatggcgtca tcaatattcc gtcaattcgt cggctggttg aaaacgccgg
     3061 atttaatggt gctattgaac tggaaatatt ctcgccttac tggtggcaga aagatattaa
     3121 cagc
//
LOCUS       DAZXUK010000348         3117 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZXUK010000348 DAZXUK010000000
VERSION     DAZXUK010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.350"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(205..1302)
                     /gene="mltA"
                     /locus_tag="ACH3L8_003975"
     CDS             complement(205..1302)
                     /gene="mltA"
                     /locus_tag="ACH3L8_003975"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFT4086862.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFVEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1511..1587
                     /locus_tag="ACH3L8_003976"
     tRNA            1511..1587
                     /locus_tag="ACH3L8_003976"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1545..1547,aa:Met,seq:cat)
     gene            1621..1697
                     /locus_tag="ACH3L8_003977"
     tRNA            1621..1697
                     /locus_tag="ACH3L8_003977"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1655..1657,aa:Met,seq:cat)
     gene            complement(1772..3025)
                     /gene="amiC"
                     /locus_tag="ACH3L8_003978"
     CDS             complement(1772..3025)
                     /gene="amiC"
                     /locus_tag="ACH3L8_003978"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFT4086863.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
BASE COUNT          748 a          867 c          747 g          755 t
ORIGIN      
        1 ctcaccctaa acgcataaat gcctgatgcg ctacgcttat caggcctaca taactcctgc
       61 aatattttgg atttgcatga tttgtaggcc ggataaggcg ttcacgccgc atccggtatg
      121 aacaacgcgc acttagtcaa taatcaggat gtgaggttac ccctcccctg tcatatccgt
      181 aaaaacggca tacagaatat cacatcagcc gctaaagacg ttacctgcgc ccggagccgt
      241 tttcagcacc caaacccgtc catagtggtt gtaccaacct gcgcggtgtc cggcttccgg
      301 cccgatccct tgatagatat cgaagtgttg gcctttgatt gcgcccccga catccaacgc
      361 caccatcaga cgcaattcgt actgaccgtt aaatttgccg ttattatcca gcagcggcac
      421 ttctgccagc aaggtagtac ctggcggaat aattgaacga tcggaggcaa cagaagcacg
      481 gccaaccagc ggcaccgcac ttgccccttt caccggtgca aaagattgcg gtttaaagaa
      541 gacgaaagac gggttctgtt ccagcagctc gcgaacctcg gcttcactgt gtgtttcgcc
      601 ccaatgacga atcgcctgca tcgacatatc ttcttttttc acttcgccac ggtcgatcag
      661 caccttacca atgctgcgat aggcatgacc gtttttccct gcatagctga aaaagttaag
      721 cggactgcca tcaccaaagt cgatataccc actaccctgc acatccataa tgaagttatc
      781 catcagagag ttgctgtaag cgaggatgta tttatcgccc aatgccccgg cgtagatctc
      841 cgcacgagac ggcagacgac cacgttttgg cggcatacgg taaataggat actggaactc
      901 gccctggcgg gtatggcgcg cctgaattac cggcgtgtaa tagcctgtaa actgcacgtt
      961 accatagttg tcggcacctt ccatctgcca ggcatcaatg ccgaactggc gcatattgcg
     1021 ggtatcaccg cctgcgcgca gccactcttg caccgcgtta taaacattac tctggttgcc
     1081 atacagacgc ggtgacgaat tacggatatg gttaatttgc tcgacaaaat caccggcgtt
     1141 aatcggcgcg ccaacggcat ctggctggtt caccagagag aaaggctggg taaatttccc
     1201 gtctttatat tgctgtccgc gatcggttgg tttggaagag caggcggcaa gcattgccac
     1261 aaccgtgccc ataagaaggt actttaccca acgtcctttc attgttctct tcttcaggtt
     1321 aaaaaataag gcgcaacgaa gataacaaac cgccgcgttt aatgaaatga gcagcctctc
     1381 cctgacgcaa attttgcaca aaaaataggc tttagtggtt tgtttttgtt caaaatcatg
     1441 ctaaatccgt gatcggggta aaaaaaaggt tgcatgaaaa cgcgagcgga gtatagtgcg
     1501 catccacgga cgcggggtgg agcagcctgg tagctcgtcg ggctcataac ccgaaggtcg
     1561 tcggttcaaa tccggccccc gcaaccaatc aaatttgatg aagtaaaagc agtacggtga
     1621 cgcggggtgg agcagcctgg tagctcgtcg ggctcataac ccgaaggtcg tcggttcaaa
     1681 tccggccccc gcaaccaatt attgaacacc ctaacgggtg tttttttgtt tctgatctcc
     1741 cataaaaaaa gcgccattca gcgccttttt atcatcccct tctcgccagc gttgccccat
     1801 cggcaaaata cgctttaatc cccgcaagaa tagactccgc aacttcctgc tggaaagtcg
     1861 ccgttttcag tttacgctct tcctcaacgt tactgataaa cgccgtttcg accagaatgg
     1921 agggaatatc tggtgccttt agtacggcaa acccggcctg ttcaacttga tttttatgca
     1981 gcttgttgat ttttccgagc ttattcagta ccgctttacc aaacttaagg ctgtcagcaa
     2041 tagtcagcga ctgcaccata tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc
     2101 tcacgccacc aatcaagtcc gaggcgttct gggtttgtgc cagatatttt gccgcagtac
     2161 tggttgcgcc tttggttgag agcgcaaaca ccgaggaacc gctcggctga cgactggtaa
     2221 aggcgtcggc atggatagag acaaacaggt cagcacgctg tttctgggct tttgctacgc
     2281 gcacttgcaa cggaatgaag atgtcttcat tgcgcgtcat gtacaccttc atattgccct
     2341 cttcctcgat cagagagcgc aaacggcgag ctatttgcaa taccacatct ttttcgcgcg
     2401 ttttgtattt ccccaccgca ccggagtctt cgccaccgtg gccagggtca agcataatga
     2461 caatcggacg atcgcgccct gctttacccg gttgtggacc actttgcgct ggcggcacct
     2521 gcttttcgag gtcgcctttg ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat
     2581 cctgtgcatt ggccggatag aggtccatca ccagacgctc tttaaacccg gcgaccggcg
     2641 caagggcaaa cagctgcggt tttacgtttt gctttaattc aaaaaccatg cgtacggttt
     2701 gcgggtcaaa ttgcccgacg cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg
     2761 ccgccatccc cttgagtacc gagttcaggt ttacatcttc gatatccacc accacgcgtt
     2821 cagggttact caacgcgaac tgcttatatt tcagctgacg atttgattct accgtcacgc
     2881 gggtgtagct ggacgcaggc cagacgcgca ccgccacgac ctggctaacc gcagccaggc
     2941 tgacctgact tacgctcaat agccacatag cgcccgcgcc ttgcagtaaa cgacgacggc
     3001 tgattgcagt gttggatcct gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa
     3061 caagtaaacg actaatttga ccggaaactt tagcgaaaga cgcataatct gtcatct
//
LOCUS       DAZXUK010000349         3112 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZXUK010000349 DAZXUK010000000
VERSION     DAZXUK010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.351"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACH3L8_003979"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACH3L8_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFT4086864.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="ACH3L8_003980"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="ACH3L8_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="ACH3L8_003981"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="ACH3L8_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFT4086865.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="ACH3L8_003982"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="ACH3L8_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFT4086866.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="ACH3L8_003983"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="ACH3L8_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAZXUK010000350         3084 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZXUK010000350 DAZXUK010000000
VERSION     DAZXUK010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.352"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..991
                     /gene="capU"
                     /locus_tag="ACH3L8_003984"
     CDS             <1..991
                     /gene="capU"
                     /locus_tag="ACH3L8_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFT4086867.1"
                     /translation="LLVCRENSKIAFEASKLGIDITFALFRNSLHIPTAWRLLGIVHG
                     FQPNAIVCHSGHDSNIVGLVRLFTRKHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPG
                     TSMKTHLEQEGCRTRVTVVPPGFDFQKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGM
                     LRPEKGHEFMLNLLFHLKMNGRQFCWLIVGSGSPELREHLQYQIDSMGMHDDVFIADN
                     VFPAAPVYRVASLVVLPSENESFGMVLAEASAFSVPVLASQIGGIPDVIQNNQTGTLL
                     PAGNKHAWMCALNDFFNDPGRFYQMARQAKQDIEERFDINKTALKILTLAKHK"
     gene            996..1946
                     /gene="virK"
                     /locus_tag="ACH3L8_003985"
     CDS             996..1946
                     /gene="virK"
                     /locus_tag="ACH3L8_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFT4086868.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            2011..2955
                     /gene="lpxM"
                     /locus_tag="ACH3L8_003986"
                     /gene_synonym="msbB"
     CDS             2011..2955
                     /gene="lpxM"
                     /locus_tag="ACH3L8_003986"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFT4086869.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT          856 a          590 c          719 g          919 t
ORIGIN      
        1 tctgcttgtc tgcagggaaa acagcaaaat tgcttttgaa gccagtaaat tggggattga
       61 tatcacattc gcgttatttc gaaacagtct tcacatccct actgcatgga gattactcgg
      121 aatagttcat ggttttcagc ccaatgcaat cgtttgtcac agtgggcatg atagcaatat
      181 tgttggttta gtacggttat tcactaggaa acatccattc agaattatca ggcaaaagac
      241 atatttgaca cgaaaaacaa aagttttttc aataaatcat ttttgcgatg aggtgattgt
      301 tcccggaaca agtatgaaga cacatctgga gcaggaagga tgtcgaaccc gggttactgt
      361 tgtgcctcca ggctttgatt tccagaaatt atacgttgat tcccgaaaca gtttgcctcc
      421 aaatgttctt tcttggctgg cgtcccgaag gggatgcccc gttattgctc aggtaggaat
      481 gttgcgcccg gaaaaagggc acgaatttat gttgaattta ctgttccatt taaaaatgaa
      541 tggacgacag ttctgttggt tgattgtggg gtctggttcg cctgaactgc gggagcattt
      601 acagtatcag attgacagta tgggcatgca tgatgatgtt tttattgctg acaatgtttt
      661 tcctgccgcc cccgtatatc gggttgccag tctggtggtt ctgccttcag aaaacgaatc
      721 gtttggtatg gtactggcag aagcatcggc attttctgtg cctgtactgg ccagtcagat
      781 tggtggaatc cctgatgtta ttcagaataa ccagaccggg acattgttac cagcaggtaa
      841 taagcacgca tggatgtgcg ccctgaatga tttttttaat gaccctgggc gtttttatca
      901 gatggctcgc caggcaaaac aggatataga agagcggttt gatattaata aaactgcgtt
      961 aaaaatactc acattagcga agcacaagta acaatatgtt ttctgtaagt aacttatcat
     1021 ttatcggttt ccttaaaagg attgtttttt cctcagattc actcccgggg aagtgggaac
     1081 acagaaaatt ccggttcatg tacattttgc gatgtgctat aaatccggtt gccagtattc
     1141 gatattatta cgaactgcgt tccttgcagt gcattgagga tattctggca atacaaccca
     1201 cgttgccagc aagaattcat cgaccttatt tgcataaagg ggggcgagcc tggtcccggg
     1261 ggcaatatat tcttgagcat tatcgctttg tgcaaaattt gccagaaaaa tattctgaat
     1321 ttttgtttcc acaaaaatcg gtatcattag tccaattcat cgggaaagat ggagaggatt
     1381 ttgatatcca gtgttctccc agtggttttg accgggaagg agagttgatg ttgtcattgt
     1441 ttttcaataa aatagtgatt gcccggttaa cattttcagt cattctgact caaaatggac
     1501 acaccgcttt tattggcgga ttacagggag cccccaaaaa taccggacct gatattattc
     1561 ggtgcgcaac ccgggcctgt tatgggcttt tcccaaaacg gattattttt gaagcatttt
     1621 gtgcgttgat gaaagcttgt aatgtctctg agtgtctggc agtcagtgag cacagtcatg
     1681 ttttccggca attgagatac tggtatcaga aacgcaaaac ctttgttgct gtctacagtg
     1741 atttctggga gtctgtagca gggaaaacct gtggtgactg gtacaaatta ccaacacagg
     1801 tggttcgaaa gccgctaagc aatatagcta gtaaaaaacg ctctgagtat cgaaaacgat
     1861 acgctttgct ggattatatc catgaaactg ctatccgctc tttggatgca tatcctgtaa
     1921 actcagaaca tcaggactta aattaaggtt tgatgtattc tctgaataaa atattaatga
     1981 tgattttggt aggggcattc gcactaaata atgaaaaaat acaaatccga gtttattcct
     2041 gaatttaaga aaaattatct ttcccctgtt tactggttta catggttcgt tttgggaatg
     2101 attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc caaaataggg
     2161 cgttgggtgg gcagattgag cagaaaagct cgtcgcaggg cgacgattaa tttatcgctt
     2221 tgtttcccgg aaaagagtga tacagaacgg gaaataattg tcgacaatat gtttgccaca
     2281 gcattgcaat ccatagtgat gatggcagaa ctggcaattc gtggtccgga aaagttccag
     2341 aaacgagtgt tctggaaagg cctcgaaatt cttgaggaaa ttcggcacaa taacagaaat
     2401 gtgatttttc tggttcccca tggctggagc gtggatattc ctgcaatgtt gctggcagct
     2461 cagggggaaa aaatggccgc catgtttcat cagcaacgaa atccagtgat tgattatgtc
     2521 tggaattctg tacggcgtaa attcgggggg cgcttacatt cccgggagga tgggataaaa
     2581 ccatttattc agtcagtacg ccagggatac tgggggtatt accttccaga tcaggatcat
     2641 ggtcctgaat acagtgaatt tgctgatttt tttgcgacct ataaagcgac attaccaatt
     2701 attggacgtc tgatgaacat cagtcaggct atgattatac cgcttttccc ggtttatgat
     2761 gaaaaaaaac atttcctgac tattgaagtt cggccaccaa tggatgcatg cattgccagc
     2821 gcggacaata aaatgattgc ccgacaaatg aacaaaacag tggaaatttt ggtggggtca
     2881 catccggaac agtatatctg ggttttaaaa ttgttaaaaa cgcgcaaatc aaacgaagcg
     2941 gacccgtacc cttgaaatta taaagtacag gtatttccac tagttgtttc ttacaggtta
     3001 ccaatcgaaa cacatccccc ttccgtgtgt tgcccgtttg gcacggagga ctgtgtagat
     3061 gcagttcgtt aaatttaatt tatg
//
LOCUS       DAZXUK010000351         3069 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZXUK010000351 DAZXUK010000000
VERSION     DAZXUK010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.353"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..498
                     /locus_tag="ACH3L8_003987"
     CDS             <1..498
                     /locus_tag="ACH3L8_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4086870.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="ACH3L8_003988"
     CDS             578..1273
                     /locus_tag="ACH3L8_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFT4086871.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="ACH3L8_003989"
     CDS             1302..>3069
                     /locus_tag="ACH3L8_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFT4086872.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPSTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          889 a          718 c          683 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccagtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAZXUK010000352         3047 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZXUK010000352 DAZXUK010000000
VERSION     DAZXUK010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.354"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..370
                     /locus_tag="ACH3L8_003990"
     CDS             <1..370
                     /locus_tag="ACH3L8_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFT4086873.1"
                     /translation="FPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGS
                     QHKWKEFYKLVLESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRC
                     FRSNTLGAKMQALRIFLSRK"
     gene            645..1841
                     /gene="kbl"
                     /locus_tag="ACH3L8_003991"
     CDS             645..1841
                     /gene="kbl"
                     /locus_tag="ACH3L8_003991"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFT4086874.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            1851..2876
                     /gene="tdh"
                     /locus_tag="ACH3L8_003992"
     CDS             1851..2876
                     /gene="tdh"
                     /locus_tag="ACH3L8_003992"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFT4086875.1"
                     /translation="MKALSKLKAEEGIWMTDVPVSELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
BASE COUNT          732 a          683 c          813 g          819 t
ORIGIN      
        1 tttcccattt gatgaaaata agatgcatct tttcactata aaaaaagggc ttactgttac
       61 atctcaacaa caggcttttg atttcatgat tggcaatcat gtatatatta ttgggggcgc
      121 aattgttggt tcacagcata aatggaaaga attctataaa ttagttctgg aaagtcaaaa
      181 aataacacta aacaataaca ttgtggacga tgaccagggc atttttgtca tgtgctatta
      241 caaacgttcc gatctcttca acctaaacta tctggggaga ggaaaatggt tcgatttgtt
      301 tcgctgcttc aggagtaaca ctttaggggc aaaaatgcag gcgctgagaa ttttcctttc
      361 aagaaaatag gtttccctcc attagtgggt agcgttttga tgccaactga gataggaaaa
      421 atgatgcttt ggcatcattt tttcttttta tatcaatgca gtaaaatcac tcatcattat
      481 gctcagccaa aactgtacag tttttctact atctgtaggt gttgagagac atcaagatac
      541 acgttattta tcctgaattt tgcagaagtg ttaacgcatc atctcgtcgc gacctataag
      601 tttgggtaat atgtgctgga atttgccctg tctggagaat cgcaatgcgt ggagaatttt
      661 atcagcagtt aaccaacgat ctggaaaccg cacgggcgga agggttgttt aaagaagagc
      721 gcattattac gtctgcgcag caagcagata tcaccgtggc tgatggaagc cacgtcatta
      781 acttttgtgc caacaactat ctcgggctgg cgaatcatcc ggatctgatt gcggcagcaa
      841 aggcgggaat ggattctcac ggtttcggca tggcttcggt gcgttttatt tgcggcactc
      901 aggacagcca taaagagctt gaacaaaaac tggcggcatt cctggggatg gaagatgcga
      961 ttctctactc ttcctgcttt gatgctaacg gtggcctgtt tgaaacgctg ctgggcgcgg
     1021 aagacgccat tatctccgac gcactgaacc acgcgtctat tattgatggt gtgcgtctgt
     1081 gtaaagctaa acgctatcgc tatgccaaca acgatatgca ggagcttgaa gctcgtctga
     1141 aagaagcgcg tgaagccggt gcgcgtcatg tgctgattgc caccgatggt gtgttctcga
     1201 tggacggcgt gattgccaat ctgaagggcg tttgcgatct ggcagataaa tacgatgccc
     1261 tggtgatggt agacgactcc cacgcggtcg gttttgtcgg tgaaaatggt cgtggttccc
     1321 atgaatactg cgatgtgatg gggcgtgtcg acatcatcac cggcacgctc ggtaaagcgc
     1381 tgggcggggc ttctggtggt tataccgcgg cgcgcaaaga agtggttgag tggctgcgcc
     1441 agcgttctcg tccgtacctg ttctccaact cgctggcacc agcaattgtc gccgcttcca
     1501 tcaaagtgct ggagatggtt gaagcgggta gcgagttgcg tgaccgtctg tgggcgaacg
     1561 cgcgtcagtt ccgtgagcaa atgtcggcgg cgggctttac cctggcggga gccgatcacg
     1621 ccattattcc ggtcatgctt ggtgatgcgg tagtggcgca gaaatttgcc cgagagctgc
     1681 aaaaagaggg catttacgtc accggtttct tctatccggt cgttccgaaa ggtcaggcgc
     1741 gtattcgtac ccagatgtct gcggcgcata cccctgagca aattacgcgt gcagtagaag
     1801 cattcacgcg tattggtaaa caactgggcg ttatcgcctg aggatgtgag atgaaagcgt
     1861 tatccaaact gaaagcggaa gagggcatct ggatgaccga cgtccctgta tcggaactcg
     1921 ggcataacga tctgctgatt aaaatccgta aaacagccat ctgcgggact gacgttcaca
     1981 tctataactg ggatgagtgg tcgcaaaaaa ccatcccggt gccgatggtc gtgggccatg
     2041 aatatgtcgg tgaagtggta ggtattggtc aggaagtgaa aggcttcaaa atcggcgatc
     2101 gcgtttctgg cgaaggccat atcacctgcg gtcactgtcg caactgtcgt ggcggtcgta
     2161 cccatctgtg ccgcaacacc attggtgtcg gtgttaaccg tccgggctgc ttcgccgaat
     2221 atctggtgat tccggcgttc aacgctttca aaatccccga caatatctcc gacgacctgg
     2281 cttccatttt tgatcccttc ggtaacgccg tgcataccgc gctgtcgttc gatctggtgg
     2341 gcgaagatgt gctggtttct ggtgcaggtc cgataggtat tatggctgcg gcggtggcga
     2401 aacacgttgg tgcacgcaat gtggtgatca ccgatgttaa cgaataccgc cttgagctgg
     2461 cgcgcaaaat gggtatcacc cgtgcggtta acgtcgcgaa agaaaacctt aatgatgtga
     2521 tggctgaact gggcatgacc gaaggctttg atgtcggtct ggaaatgtcc ggcgcgccgc
     2581 cagcgtttcg taccatgctc gataccatga accacggcgg tcgtattgcg atgctgggta
     2641 ttccaccgtc tgatatgtct atcgactgga ccaaagtgat ctttaaaggc ttgttcatta
     2701 aaggcattta cggtcgtgag atgtttgaaa cctggtacaa gatggcggcg ctgattcagt
     2761 ctggcctcga tctctcgccg atcattaccc atcgtttctc tatcgatgat ttccagaagg
     2821 gctttgacgc tatgcgttcg ggccagtccg ggaaagttat tctgagctgg gattaacacg
     2881 aacaagggct ggtattccag cccttttatc tgaggataat ctgttaatta tgtaaaatcc
     2941 tgtcagtgta ataaagagtt cgtaattgcg ctgatctttt atatagctgt tctcattatc
     3001 tctctaccct gaagtgactc tctcacctgt aaaaataata tctcaca
//
LOCUS       DAZXUK010000353         3028 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZXUK010000353 DAZXUK010000000
VERSION     DAZXUK010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.355"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..856)
                     /locus_tag="ACH3L8_003993"
     CDS             complement(<1..856)
                     /locus_tag="ACH3L8_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFT4086876.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNV"
     gene            964..1242
                     /locus_tag="ACH3L8_003994"
     CDS             964..1242
                     /locus_tag="ACH3L8_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086877.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACH3L8_003995"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACH3L8_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFT4086878.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..>3028)
                     /gene="hdeD"
                     /locus_tag="ACH3L8_003996"
     CDS             complement(2569..>3028)
                     /gene="hdeD"
                     /locus_tag="ACH3L8_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFT4086879.1"
                     /translation="ISFPFVSGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSG
                     FLVAVAYLLIGYFFIRAPELGIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLV
                     IGVLDIVIAWIFLGATPMVSVTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          855 a          667 c          667 g          839 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgata
//
LOCUS       DAZXUK010000354         2962 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZXUK010000354 DAZXUK010000000
VERSION     DAZXUK010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.356"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(92..229)
                     /gene="yjdI"
                     /locus_tag="ACH3L8_003997"
                     /pseudo
     CDS             complement(92..229)
                     /gene="yjdI"
                     /locus_tag="ACH3L8_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
     gene            410..2041
                     /locus_tag="ACH3L8_003998"
     CDS             410..2041
                     /locus_tag="ACH3L8_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFT4086880.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2038..2757
                     /gene="dcuR"
                     /locus_tag="ACH3L8_003999"
     CDS             2038..2757
                     /gene="dcuR"
                     /locus_tag="ACH3L8_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFT4086881.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
BASE COUNT          810 a          724 c          701 g          727 t
ORIGIN      
        1 ctccaatgac tagtctaaaa actagtatta agactatcac ttaaataagt gatactggtt
       61 gtctggagat tcagggggcc agtctacacg actttcgttt gagattaaat aacttgccgt
      121 tgccacgtac gcaattgcca gaatgctgac atatcgcagt gttgaaatag acatcgatct
      181 tttcgccggt ataacagcga taacccccgt ccagtagcgc ctgatccatg accttccctc
      241 tctcatcagt atgattatcg ctataagcat agccccggaa tcttcataca gcactggcag
      301 tttccgttgc cagagtctct tcccctatat agccaatcat ttttactccc tttgaattac
      361 ccgcctcatc agagataatg cttaagaaat ttgtcacaca aggaagctga tgagacattc
      421 attgccctac cacatattac gcaaacgtcc gatgaaattg agtaccactg tgatcttaat
      481 ggtcagcgcg gtactgttct cggtgctatt ggtggtgcat ctgatttact tctcgcaaat
      541 cagtgatatg acgcgagacg ggctggccaa caaggcactg gcagtggcgc gtaccctcgc
      601 cgactcgccg gaaatccgtc agggcttgca gaaaaaaccg caggagagtg gcatccaggc
      661 catcgcggaa gccgtgcgca aacgcaacga tctgctgttt attgtcgtta ccgatatgca
      721 aagtcttcgc tactcacatc ctgaagccca gcgtattggt cagccattta aaggtgatga
      781 catccttaat gcgctgaatg gcgaagaaaa tgtcgctatc aatcgcggtt ttctggcgca
      841 ggctttacgc gtatttaccc ccatctacga tgaaaatcat aaacaaattg gcgtggtggc
      901 gatcggcctt gagttaagcc gcgtgaccca acagatcaat gacagtcgct ggagcattat
      961 ctggtcggta ttatttggca tgctggtcgg actgattggc acctgcattc tggttaaggt
     1021 actgaaaaaa atccttttcg gcctggaacc ctacgaaatc tccacgctgt ttgagcaacg
     1081 ccaggccatg ttgcagtcta tcaaagaagg cgtcgttgcc gtggacgatc gcggcgaggt
     1141 cacgctgatc aacgatgccg cacaagaatt gctgaattac cgtaagtcgc aggacgatga
     1201 gaaactgtcg acgctcagcc actcatggtc acaggtggta gatgtctcgg aagtgttacg
     1261 cgacggtacg ccgcgccgcg acgaagagat tacgattaaa gaccggctat tactgatcaa
     1321 caccgttccg gtgcgcagta atggcgttat catcggtgcc atttcaacct tcagggacaa
     1381 aactgaagtt cgtaaactga tgcagcgact cgacggtctg gtcaactatg ctgacgcact
     1441 tcgtgaacga tcccacgaat ttatgaataa attgcatgtg attctcggat tattgcatct
     1501 gaagagttat aagcagttgg aagattacat tctcaaaaca gccaataact atcaggaaga
     1561 gattggctct ctgctgggta agatcaaatc tccggttatc gctggttttt taatcagcaa
     1621 gattaaccgc gcgaccgatt taggccatac gctgatttta aacagtgaaa gccagctgcc
     1681 agacagcggc agtgaggacc aggtcgcgac gctgattacc acattgggaa atctgataga
     1741 aaacgcgctg gaagcattag ggccggaacc cggtggcgaa attagcgtaa cattgcacta
     1801 ccgtcacggc tggctgcact gcgaagttaa cgatgatgga ccggggatcg cacccgacaa
     1861 aatcgatcac atttttgaca aaggtgtctc gacaaaagga agcgagcgag gcgtcggttt
     1921 agcacttgtc aaacaacagg tagaaaatct cggcggcagc atcgccgtgg aatcggaacc
     1981 ggggattttc acacaatttt ttgtccagat accctgggac ggggagaggt cgaacagatg
     2041 atcaatgtat taattatcga tgacgacgca atggtcgcgg agctgaatcg ccgatacgta
     2101 gcacaaatcc caggctttca atgctgtgga acagcctcga cgctggagaa agccaaagag
     2161 attatcttca atagcgatgc gcctatcgac ctgatattgc tcgatatcta tatgcaaaaa
     2221 gagaacgggc tcgatttact gcctgtcctg cataacgcgc gttgcaaaag tgatgtgatt
     2281 gtcatctcct ccgcagccga tgcggcaacc attaaagatt cgctgcatta cggtgtcgtg
     2341 gattacctga tcaaaccctt ccaggcttcc cgctttgaag aggcgctcac cggctggcgg
     2401 caaaagaaaa tggcgctgga aaaacatcag tattatgacc aggccgagct tgatcagcta
     2461 atccacggca gcagctccaa cgaacaggat cctcgtcgct tgccaaaagg cttaacgccg
     2521 cagacgttac gcacgctgtg ccagtggatt gacgcgcatc aggactatga gttttcaacc
     2581 gacgagctgg ctaacgaggt taacatctcg cgtgtttcct gccgaaaata cctcatctgg
     2641 ctggtcaact gccacatctt gttcaccagt atccattatg gcgtcacggg gcgtccggtt
     2701 taccgttacc gtattcaggc agaacactac tcactgctga aacaatattg ccaataacgg
     2761 atcgctggtt atctgtaagt aataattatt accgtaatgc aaaatacccc tggcacaagt
     2821 tacatcgtta atcagtaaaa cttgtgccag atcaaatata attatccctc catcatcgtt
     2881 aaaaattaat atctcttcag atgaacggtg tttttaattt caaaacgata acaaaagtta
     2941 attaactatt atgtcacccg ca
//
LOCUS       DAZXUK010000355         2951 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZXUK010000355 DAZXUK010000000
VERSION     DAZXUK010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.357"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="ACH3L8_004000"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="ACH3L8_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFT4086882.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="ACH3L8_004001"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="ACH3L8_004001"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFT4086883.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="ACH3L8_004002"
     CDS             2603..>2951
                     /locus_tag="ACH3L8_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFT4086884.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAZXUK010000356         2948 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZXUK010000356 DAZXUK010000000
VERSION     DAZXUK010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.358"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            302..1687
                     /gene="dcuC"
                     /locus_tag="ACH3L8_004003"
     CDS             302..1687
                     /gene="dcuC"
                     /locus_tag="ACH3L8_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFT4086885.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1728..2069)
                     /locus_tag="ACH3L8_004004"
                     /pseudo
     CDS             complement(1728..2069)
                     /locus_tag="ACH3L8_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2407..2916)
                     /locus_tag="ACH3L8_004005"
     CDS             complement(2407..2916)
                     /locus_tag="ACH3L8_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFT4086886.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
BASE COUNT          681 a          671 c          689 g          907 t
ORIGIN      
        1 atatatatcg tcaacccatg ctcatattag attatttttt gagcgcacag ccgcagctaa
       61 caccattgca attaacaaat ttgcatcaat ccaccatcaa tttacacaca ttattatgtg
      121 ataattgcca accgctaaat atgcgttttg ttatctatgt ataaaaacag caacttcaat
      181 gtcttaatgg cagtttttct tgattttaat cagcattcat cgccaattta ttgggcatat
      241 tttttcctta agctttagga agtttttatt atttactttg ggcctggaga caggaaaaat
      301 tatgctgaca ttcattgaac tccttattgg ggttgtggtt attgtgggtg tggctcgcta
      361 catcattaaa gggtattccg ccactggtgt gttatttgtc ggtggcctgt tattgctgat
      421 tatcagtgcc attatggggc acaaagtgtt accgtccagc caggcttcaa caggctacag
      481 cgccacggat atcgttgaat acgttaaaat attactaatg agccgcggcg gcgacctcgg
      541 catgatgatt atgatgctgt gtggctttgc cgcttacatg acccatatcg gcgcgaatga
      601 tatggtggtc aagctggcgt caaaaccatt gcagtatatt aactcccctt acctgctgat
      661 gattgccgcc tattttgttg cttgtctgat gtcactggcc gtctcttccg caaccggtct
      721 gggggttttg ctgatggcaa ccctgtttcc ggtgatggta aacgttggta tcagtcgtgg
      781 cgcagcagct gccatttgtg cctccccggc ggcgattatt ctcgcaccga cttcagggga
      841 tgtggtgctg gcggcgcagg cttccgaaat gtcgctgatt gacttcgcct tcaaaacgac
      901 gctgcctatc tcaattgctg caattatcgg catggcgatc gcccacttct tctggcaacg
      961 ttatctggat aaaaaagagc acatctctca tgaaatgtta gatgtcagtg aaatcaccac
     1021 cactgccccc gcgttttatg ccattttgcc gttcacgccg atcatcggtg tactgatttt
     1081 tgacggtaaa tggggtccgc aattacacat catcactatt ctggtgattt gtatgctgat
     1141 tgcctccatt ctggagttcc tccgcagctt taatacccag aaagttttct ctggtctgga
     1201 agtggcttat cgcgggatgg cagatgcgtt tgctaacgtg gtgatgctgc tggttgccgc
     1261 tggggtattc gctcaggggc ttagcaccat cggctttatt caaagtctga tttctatcgc
     1321 tacctcgttt ggttcggcga gtatcatcct gatgctggta ttggtgattc tgaccatgct
     1381 ggcggcagtc acgaccggtt caggcaatgc gccgttttat gcgtttgttg agatgatccc
     1441 gaaactggcg cactcttccg gcattaaccc ggcgtatttg actatcccga tgctgcaggc
     1501 gtcaaacctg ggtcgtaccc tatcacccgt ttctggcgta gtcgttgcgg ttgccgggat
     1561 ggcgaaaatc tcaccatttg aagtcgtaaa acgcacctcg gtgccggtgc ttgttgggct
     1621 agtgattgtt atcgttgcta cagagctgat ggtgccagga acggcagcag cggtcacagg
     1681 caagtaaata gtaatgccgg aggcaagttt cctccggctt tatcatgtca cccactgtgg
     1741 tatatgcgtt gtggtctgcc aactttgccg tgaacaattt cagcaataat cagatggcgg
     1801 ctggcgcaat attcaagata acgcctggca gtggtgcggc tgatggtcag tgcctgcgcc
     1861 accgtttctg ccgtatgttg cacaccaggc tctttaaaca gttttcgcac cgcgtttagc
     1921 gtcaaggggt caatgccggt cggcagttcg tctttgggtt caccgcgagc ataggcatta
     1981 aacatctcat caatttgctt ctggctggcg ctatcaatac tttccagcat atgtttacgc
     2041 tggcggaaac gggttagcgt ttgccccagt gtattgattc acttgaagta cgaaaaaacc
     2101 gggagaacat tggattattc gggatctgat gggattagat ttggtggggc ttgcaagcct
     2161 gtagtacaaa ttttaggcgt taaccaatga aacgcgaaag atagtaaaaa attgcttttg
     2221 tttcattgaa aaaacgaaaa acaaaaacac tataaatcat ttcaatcata gcttcaaaaa
     2281 acgttcaaaa ccgataacaa ccaggctgtc accaaatgac tcatatcaca aatcagctca
     2341 tgccgtttag gtatgttaca tgtgtgatta tgtgaggtga agtatgtttt agctggctca
     2401 tggttgttat acggcttttt tcgcatcttg tggttccaac aaaggagcta caggctcttc
     2461 ctgtttatga acaccataca ttgacaaaat ctcttggcga gtttctggag gtaatttatg
     2521 cagcgccaaa gctgtttgta gtgtttggcg atcaataccc agcgttgcca atagggtgtc
     2581 aacggagatg gaggggcgtt cttgcattaa tccggaaagc ccatatttag ccatgtttag
     2641 ctctatctct cggacttcat tttctcctag tttattgatt aacagggaga acattttcaa
     2701 tgtatcagca ttcatttctt cgatagcaat tttttgcata gccggtattg ccgggatata
     2761 ttcaacccca ggcccgcgac gcccttcttg tgaacgcttc ggccatcctt ctttctttgc
     2821 tcgtttatgc accccctgta ttgtcccagg cattccatcc agttcagtaa tttgtttggg
     2881 tgtcatccac tggtttatct catttgcgct tttcataaac taagtttacc agatttatct
     2941 atataaaa
//
LOCUS       DAZXUK010000357         2932 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZXUK010000357 DAZXUK010000000
VERSION     DAZXUK010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.359"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="ACH3L8_004006"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="ACH3L8_004006"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFT4086887.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="ACH3L8_004007"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="ACH3L8_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFT4086888.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="ACH3L8_004008"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="ACH3L8_004008"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFT4086889.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2756..>2932
                     /locus_tag="ACH3L8_004009"
     CDS             2756..>2932
                     /locus_tag="ACH3L8_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086890.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELW"
BASE COUNT          660 a          800 c          805 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cacatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt gg
//
LOCUS       DAZXUK010000358         2929 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZXUK010000358 DAZXUK010000000
VERSION     DAZXUK010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.360"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACH3L8_004010"
     CDS             complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACH3L8_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFT4086891.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGH"
     gene            348..641
                     /locus_tag="ACH3L8_004011"
     CDS             348..641
                     /locus_tag="ACH3L8_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFT4086892.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="ACH3L8_004012"
     CDS             713..1372
                     /locus_tag="ACH3L8_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFT4086893.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="ACH3L8_004013"
     CDS             1464..1910
                     /locus_tag="ACH3L8_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFT4086894.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..2929)
                     /gene="hlyE"
                     /locus_tag="ACH3L8_004014"
                     /pseudo
     CDS             complement(2117..2929)
                     /gene="hlyE"
                     /locus_tag="ACH3L8_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT          765 a          633 c          710 g          821 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttatgata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagat
//
LOCUS       DAZXUK010000359         2915 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZXUK010000359 DAZXUK010000000
VERSION     DAZXUK010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.361"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(84..227)
                     /locus_tag="ACH3L8_004015"
                     /pseudo
     CDS             complement(84..227)
                     /locus_tag="ACH3L8_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            198..482
                     /locus_tag="ACH3L8_004016"
     CDS             198..482
                     /locus_tag="ACH3L8_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086895.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            complement(484..1056)
                     /locus_tag="ACH3L8_004017"
     CDS             complement(484..1056)
                     /locus_tag="ACH3L8_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086896.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(1142..1348)
                     /locus_tag="ACH3L8_004018"
     CDS             complement(1142..1348)
                     /locus_tag="ACH3L8_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFT4086897.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            complement(1734..2525)
                     /locus_tag="ACH3L8_004019"
     CDS             complement(1734..2525)
                     /locus_tag="ACH3L8_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086898.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
BASE COUNT          801 a          618 c          685 g          811 t
ORIGIN      
        1 agggatatgg aaaagtgtat agaatcccat aaaaaatcga tattgttcag ttgacgtata
       61 tttctggagg atgactccag tcatcagaac atatgttttt cgatgaatga cccaataatt
      121 ttttcgtgaa tttccacagc acgtgaaaag taaatcgttt ttcggtccag tcgtttgatg
      181 cgggaacgga gtgtcagatg gtggtgctca atacgccggg taaatatcaa tacgatttga
      241 ggaaccattt gctggttaat ctctttccga tagcattgga tgcagaaata actacatcca
      301 atgtaaataa gcatcaattg cacagaagca atatacctgc ggccctctcc ggaaattccg
      361 gtatcactga ctacgtaccg ttttcagcgg aacgaaatgc ggcgatggtc atttggcgga
      421 cagccataga tagccgtccg gctgtgtgta acgatgagga aggaaccccg ggaacgttat
      481 gaatcagcag gcgtataaca ggtactcgtc aatcatcagt tccagctcgc gcttttcccg
      541 tttgcgcgcc acagcccagt tttcaagctc tgcatgtgat ttatgcttta actcttcagt
      601 ccagggctgc atggagtggg cacacatcag atcataccag cacagccata tcagcgtgtg
      661 gtagagatca cgatcgtcga aacgccggag caggctgttc agcctgtttg ccagccggtt
      721 ttcacccaga cgatgtagct cactgagtaa aacgccgccc agattaaaat gtgtaagcag
      781 catgatttca tgggtctgaa tgctggtcat taacccttcc tgaatggcag gaatgaacgg
      841 tggtgttccc gggttgacga cctgatagag tccctggggg gccaccgaaa tgacggagcc
      901 tttttccggc agaaaaaagt gtgccagggt cagacggtga taaccatgaa gatgcggaga
      961 ccccattcca tgagcgacga taagcctcgt cacgcttgtc atccatttct gatgctggat
     1021 gagcagcagg tcacacctgt tttcaagttg ttgcataata ttctctcctt tagcgcgcag
     1081 caaaatgcct ccgggggccg gatcatgcct ttgctgcggt ggaacggata acggtcagga
     1141 attacggacg ggactgtgca atacgcgcct gcagccaggc ttccacttcg cttttcagcc
     1201 agcgggagct acggcccagt ttgatggggg gcggaaaggc tccatgcttg atgagtttat
     1261 aaaaccactt atcggtcagg ccggtcagct gagtgataaa cgacatgtcg accatctggt
     1321 catccatcag tgaaactggg gtattcatga gaatgttcct ccggatgttg ttaatctcaa
     1381 ccggcgttgg tgatgaaaac gacgcaccac cttcaccggc ataacaaccg gggagaaacg
     1441 taaaaaatga gtaggggaat attcctgcca ggagagaaac tgttgtgtac aggcaataca
     1501 ggttgtctgt tttgtcagtg aatccggtat aaacggaaga tgattttgtg ttgccggaat
     1561 atgggatggc gttttgccgt gacgggatca gtattttcag tgatgattat tactgcacag
     1621 gggagtatcg gagataaacc acgccctgta cctgccagag cggtggtttg tatttactga
     1681 acggaagata aagcagaggt aaacactgtc gcggtgaagg tagaaggctg aaattattct
     1741 ctgtaattac aaacaacagt gatcttatcc ggttttttta ccgttacggg tttcagaggg
     1801 tcaattttat atacaggggt aatgagcacg ccggaattta tggaaaaaag agggatccag
     1861 caataaacgt ccggatatgt ctgtgtctcc ggaatacctt ttgcgccaga gaccatcaga
     1921 tctgtaacgc tcacaaaaaa tgactgccgc aaatcaaaaa agtactcgtt atatatcaca
     1981 tcctttgcca gcaggatgac tgcattacca acagccgctt ttacaggtat tttatcagat
     2041 ggattatcgt gattgtatat tgttctttcg atataaataa aacaggcata tttcgtcttc
     2101 tccccggata gctgcatctg gtattcgcaa atgtctttga tacaccgact ttcaaccctt
     2161 ccaatcatct tatcccggcg atagtgtctt ttatgtttca gcgcgtacag aacaccattt
     2221 gaactgtcaa actccaggat tcttctggtg aaaagtttgt tttcccagat ttccagacaa
     2281 tacttctctg cactgacgac attgcgtttg cgaggaagat cccgttgata cactccattt
     2341 aataagcgtg actgcagttc gcctgcaagt tgataataac agttaatatc attaaggaca
     2401 ggtgctgtgg cgctgagtaa tgcgttgatt accttagagc gcgaaagttt gtgggtatga
     2461 cagtactcat ccacaattcg cagcgtctca tcactggtca tactgaacgt tatacgtgtt
     2521 gtcatgatga agttttctgt taatcatatt ggttgagatg cataacattt cataaaaaaa
     2581 cctgttaatt catgcaatta attttttaat gaattttatc ctcaagtgat ggaaagcagt
     2641 gttactgcca ctgtactcag tataactcta tcacacagaa tcacagaaaa tgaagcattg
     2701 ctcaggggga cactcatacg agttctctga actgtgataa ccagtatttt tcattttggg
     2761 tgatttgttt atcgttacag ctacaggtag ttcatgtacc ataaaaaaat aattaaggtt
     2821 aattagtatt atttacagaa gtatggtcat gtaaatgatc ctgtgattat accttgtaaa
     2881 tacaccttat aacccaatcc tgtagacgga tctct
//
LOCUS       DAZXUK010000360         2894 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZXUK010000360 DAZXUK010000000
VERSION     DAZXUK010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.362"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..308)
                     /locus_tag="ACH3L8_004020"
     CDS             complement(<1..308)
                     /locus_tag="ACH3L8_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFT4086899.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     "
     gene            complement(355..1074)
                     /gene="btsR"
                     /locus_tag="ACH3L8_004021"
                     /gene_synonym="yehT"
     CDS             complement(355..1074)
                     /gene="btsR"
                     /locus_tag="ACH3L8_004021"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFT4086900.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="ACH3L8_004022"
     CDS             complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="ACH3L8_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFT4086901.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          748 a          774 c          657 g          715 t
ORIGIN      
        1 tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg
       61 cgacgttccg gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg
      121 ccattgagga atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct
      181 ttacgtagcc agacggcgat ctgttccgcg gtggcttcga cattatccag cgccagaata
      241 cgcaccaggt cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta
      301 cttatcatga ggccttcagg tgttgatgag gcaaaaagcc attttagcag tcttttacag
      361 gccaatcgcc tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt
      421 acgcaaaatc aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag
      481 gttaaccaga tactgacgat ggcagcgcag tagtggtgta cgactttcca aggtacgtaa
      541 tgtcaattcg gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg
      601 actgctgaca aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca
      661 agggataaat ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg
      721 ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag
      781 ataatcaaag gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa
      841 aacaatatac gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg
      901 cggcatctgg atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc
      961 ttccacggcg tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat
     1021 acgcaggttc tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg
     1081 tccctccatg gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt
     1141 attccatagt catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc
     1201 ccactggcat tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa
     1261 tgttgcccct cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc
     1321 ccatgtttaa tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg
     1381 gataattctt gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca
     1441 atttgcagat aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc
     1501 cgctttaagt ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg
     1561 ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc
     1621 acctgggcgt gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat
     1681 tgccctgcaa gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt
     1741 attgaactga ataaccggtt tttggcttca tacaatttga tggtgcccat cacgcgctga
     1801 ttttcgccac gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag
     1861 caacgataag gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt
     1921 aaggtgtaag tcgaagaaat cggtttgccg ggtaaatggt ggtcgtcacc aattccggta
     1981 aaggccagca atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac
     2041 agtacctgag ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc
     2101 gtcgaggctg ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc
     2161 gcgcgtttat cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc
     2221 attggcgcag caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgca
     2281 aggatgatca gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg
     2341 ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc
     2401 aatccttcaa cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat
     2461 cgatgtaagc cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg
     2521 cccattaccg cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac
     2581 caggtgccca tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga
     2641 cgaaccgtga cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg
     2701 actaaaaaaa cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc
     2761 gcaaaccaca cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga
     2821 actcttgcag aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata
     2881 aaatcaccct atag
//
LOCUS       DAZXUK010000361         2891 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZXUK010000361 DAZXUK010000000
VERSION     DAZXUK010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.363"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1541
                     /locus_tag="ACH3L8_004023"
     CDS             <1..1541
                     /locus_tag="ACH3L8_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFT4086902.1"
                     /translation="PTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVD
                     TYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGV
                     YSNTDAQDGIPLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFT
                     TFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVV
                     DRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKR
                     ILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKG
                     KLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWI
                     TSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVEN
                     NQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKAL
                     SEAK"
     gene            1593..2750
                     /locus_tag="ACH3L8_004024"
     CDS             1593..2750
                     /locus_tag="ACH3L8_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFT4086903.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="ACH3L8_004025"
     CDS             complement(2716..2838)
                     /locus_tag="ACH3L8_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086904.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          879 a          628 c          584 g          800 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt a
//
LOCUS       DAZXUK010000362         2847 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZXUK010000362 DAZXUK010000000
VERSION     DAZXUK010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.364"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..170)
                     /locus_tag="ACH3L8_004026"
     CDS             complement(<1..170)
                     /locus_tag="ACH3L8_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086905.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="ACH3L8_004027"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="ACH3L8_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFT4086906.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
BASE COUNT          680 a          768 c          797 g          602 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaac
//
LOCUS       DAZXUK010000363         2834 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZXUK010000363 DAZXUK010000000
VERSION     DAZXUK010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.365"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACH3L8_004028"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACH3L8_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFT4086907.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="ACH3L8_004029"
     tRNA            complement(621..695)
                     /locus_tag="ACH3L8_004029"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="ACH3L8_004030"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="ACH3L8_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="ACH3L8_004031"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="ACH3L8_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFT4086908.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          679 a          693 c          683 g          779 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccga
//
LOCUS       DAZXUK010000364         2820 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZXUK010000364 DAZXUK010000000
VERSION     DAZXUK010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.366"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            31..683
                     /gene="pphB"
                     /locus_tag="ACH3L8_004032"
                     /pseudo
     CDS             31..683
                     /gene="pphB"
                     /locus_tag="ACH3L8_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(734..1501)
                     /gene="ygbI"
                     /locus_tag="ACH3L8_004033"
     CDS             complement(734..1501)
                     /gene="ygbI"
                     /locus_tag="ACH3L8_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFT4086909.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1697..2605
                     /gene="ltnD"
                     /locus_tag="ACH3L8_004034"
                     /pseudo
     CDS             1697..2605
                     /gene="ltnD"
                     /locus_tag="ACH3L8_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
     gene            2602..>2820
                     /locus_tag="ACH3L8_004035"
     CDS             2602..>2820
                     /locus_tag="ACH3L8_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFT4086910.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT          713 a          694 c          680 g          733 t
ORIGIN      
        1 ataggaaagc ttattttaca gggtaaaacc atgccatcta cacgctacca aaaaatcaat
       61 gcccatcact atcgccatat atgggtcgtt ggtgatattc atggtgaata tcagttatta
      121 caatcccgct tacatcaact ctcttttttc cccgaaaccg acttacttat ttctgtcggc
      181 gataatattg atcgtgggcc ggagagtctt gacgtcctgc gcctgctaaa ccagccctgg
      241 tttatctccg ttaaaggcaa ccacgaagca atggcgctgg atgcgttcgc gaccggcgat
      301 ggcaatatgt ggcttgccag cggtggtgac tggtttttcg atttaaatga ttgcaacaag
      361 aggcaatagt tctgttgctg aaattccatc accttccaca tattattgaa atcactaacg
      421 acataataaa atatgtcatc gcacatgcag attatccggg tgatgaatat ctctttggca
      481 aagaaatagc ggagagcgaa ttactctggc ctgtttatcg tgtgcagaaa tcgcttaatg
      541 gcgagttaca acaaataaac ggcgctgatt attttatatt tggacatatg atgtttgata
      601 acattcagac gttcgctaac cagatttata ttgataccgg atcgccgaaa agtgggcggc
      661 tgtcatttta taaaataagg tagtctcatg cttcttctgt gaagcatgag taaccagatg
      721 ttatttcagg ccattattca tttttcgcta ccagcaaaga gagatcctgc ttcgccagcg
      781 cgcgactggc actctccggc aaaccgtcgt ctgtaataat ctgatcaaac tcgcttaatg
      841 gtaacgccag ccatgtcgcc acctgaccat atttcgtcgc atcacagacc aaaactcgct
      901 ggcggctggc actggcaatc gcccgtttca ccgtgacttt atcttccgct ggcgtagaaa
      961 tcccccgcac actccatgac gatgcagaaa taaaagcctg atcaatcatc aggctgcaca
     1021 gcatggtcgc agcggcttcc ccgacacagg aacggttttc ccgacacact gcaccgccag
     1081 tgtgaataat tgtgcaatta ctgttgtcga gcaagtagtt cgcaataacg aaatcgtttg
     1141 tgaccacagt cagtgactcc atgtgaatca gatgctgtgc tatcgctaac gtggtcgttc
     1201 ccgcatccag atagatacaa cttcccggca gaacaagact tgccgccagc ttgccaatag
     1261 ccgctttttg cgtcattgcc agcgcagttt ttacctgatg agaaggttca tgcgccacgc
     1321 gtcccggaga ctggacgcct ccggacacca gcacaacggc tccctgctgc tccagttttt
     1381 gtaaatcccg acgaatggtc atatgtgaca cattaattct gtccgttagt tcagcaatac
     1441 tgacaatgcc tttttcagct accatctcaa ggatgatttg gcgacgctct acgggtatca
     1501 acttttgctc cttcctttgt cctgctgaca ttctacgcta tttgcctgcg aaacgtgcgc
     1561 ggcgcaacta acgcttagtt cacataaaat aacacaaaat gttaatttat gtgaatcatc
     1621 tcaccatatc gttatctccc agcgcttatg ttcacaatat caaacaaaat atcacttaaa
     1681 ttaacaagga gagcagatga aaacgggatc tgagtttcat gtcggtatcg ttggcttagg
     1741 gtcaatggga atgggagcag cactgtcatg tgtccgcgca ggtttttcta cctggggcgc
     1801 agacctgaac agcaatgcct gcgctacgtt gaaagaggca ggtgcttgcg gggtttctga
     1861 taacgccgcg acgtttgccg aaaaactgga cgctctgctg gtgctggtgg tcaatgcggc
     1921 ctaggttaaa caggtgctgt ttggtgaaac aggcgttgca caacatctga aacccggtac
     1981 ggcagtaatg gtttcttcca ctatcgctag tgctgatgcg caagaaattg ctaccgctct
     2041 ggctggattc gatctggaaa tgctggatgc gccagtttct ggtggtgcag taaaagccgc
     2101 taacggtgaa atgactgtca tggcctccgg tagcgatatt gcctttgaac gactggtacc
     2161 cgtgctggaa gccgttgccg gaaaagttta tcgcataggt gcagaaccgg gactaggttc
     2221 gaccgtaaaa attattcacc agttgttagc gggcgtacat attgctgccg gagccgaagc
     2281 gatggcactt gcagcccgtg cggggatccc gctggatgtg atgtatgacg tcgtgaccaa
     2341 tgccgccgga aattcctgga tgttcgaaaa ccggatgcgt catgtggtgg atggcgatta
     2401 caccccgcat tcagccgtcg atatttttgt taaggatctt ggtctggttg ccgatacagc
     2461 caaagccctg cacttcccgc tgccattggc ctcaacagca ttgaatatgt tcaccagcgc
     2521 cagtaacgcg ggttacggga aagaagacga tagcgcagtt atcaagattt tctctggcat
     2581 cactctaccg ggagcgaaat catgatcaag attggcgtta tcgccgatga ttttaccggc
     2641 gcgacggata tcgccagttt tctggtggaa aacggtctac caacggtaca aattaacggt
     2701 gttccaacag gtaaaatgcc ggaagcaatc gacgcactgg tgatcagcct gaaaacgcgc
     2761 tcctgtccgg tggttgaagc cacacagcaa tcgctggcgg ctctgagctg gttgcaacag
//
LOCUS       DAZXUK010000365         2807 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZXUK010000365 DAZXUK010000000
VERSION     DAZXUK010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.367"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            6..81
                     /locus_tag="ACH3L8_004036"
     tRNA            6..81
                     /locus_tag="ACH3L8_004036"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACH3L8_004037"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACH3L8_004037"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACH3L8_004038"
     CDS             complement(299..1057)
                     /locus_tag="ACH3L8_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFT4086911.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACH3L8_004039"
     CDS             complement(1065..2168)
                     /locus_tag="ACH3L8_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFT4086912.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGVCWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="ACH3L8_004040"
     CDS             complement(2178..>2807)
                     /locus_tag="ACH3L8_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFT4086913.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          764 a          739 c          674 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acagacaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAZXUK010000366         2791 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZXUK010000366 DAZXUK010000000
VERSION     DAZXUK010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.368"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..198
                     /locus_tag="ACH3L8_004041"
     CDS             <1..198
                     /locus_tag="ACH3L8_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFT4086914.1"
                     /translation="KLVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIK
                     QRGVNVFIQDVPGDQKEQIPD"
     gene            237..1040
                     /gene="agaC"
                     /locus_tag="ACH3L8_004042"
     CDS             237..1040
                     /gene="agaC"
                     /locus_tag="ACH3L8_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFT4086915.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1030..1821
                     /gene="agaD"
                     /locus_tag="ACH3L8_004043"
     CDS             1030..1821
                     /gene="agaD"
                     /locus_tag="ACH3L8_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFT4086916.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASLGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1822..2577
                     /locus_tag="ACH3L8_004044"
     CDS             1822..2577
                     /locus_tag="ACH3L8_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFT4086917.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          693 a          649 c          689 g          760 t
ORIGIN      
        1 aaattggtag aaggtggcat tgacctgaaa gatgtcaacg tcggcaatat gcatttctcg
       61 gaagggaaaa agcaaatcag cagtaaagtt tatgtcgatg accaggatct cgcggactta
      121 cgttttatta aacaacgtgg cgtgaatgtt ttcattcagg acgtccctgg cgatcaaaaa
      181 gaacaaatcc ctgactaaat ctaaaatcgc cttaatattg gtttgaggta ataaaaatgc
      241 atgaaataac cctacttcag ggattatccc tagcggcgtt agttttcgtt ctggggattg
      301 atttttggct ggaagcctta tttttattcc gcccgataat cgtttgtacc ctaactggcg
      361 ctattctcgg tgatattcag actggcttaa ttaccggtgg tctgacagag ttggctttcg
      421 ccggattaac ccctgcaggt ggtgttcagc cgcccaaccc gattatggcg ggtctgatga
      481 ccaccgtcat tgcatggtct acgggcgttg atgccaaaac agcaattggt cttggcctgc
      541 cgtttagttt gttaatgcag tacgtcattc tgttcttcta ttccgctttc tcattattta
      601 tgaccaaagc cgataaatgc gcgaaagagg cggatacggc agcgttttcc cggcttaact
      661 ggacaacgat gctcatcgtc gcttcagcgt atgcggtgat tgctttcctc tgtacttacc
      721 tggcacaagg ggcgatgcag gcgctggtga aagcgatgcc cgcctggttg acccacggct
      781 ttgaagtggc tggcggtatt ctgcctgccg ttggttttgg cttgctgctg cgcgtaatgt
      841 tcaaagcgca atatatccct tacctgatcg ccggtttcct gtttgtttgc tacatccggg
      901 tcagcaacct gttgccggtt gccgtactgg gcgcaggctt tgcggtgtat gagtttttca
      961 atgcgaaatc ccggcagcaa gcgcaaccgc agcccgttgc cagtaaaaat gaagaagagg
     1021 actacagcaa tgggatctga aatcagtaaa aaagatatca cccgtctggg ctttcgttcg
     1081 tcgctgctgc aagcgagctt taactacgaa aggatgcagg cgggcggttt tacctgggcg
     1141 atgttgccga tcctgaaaaa gatttataag gacgacaaac cgggcttaag cgcggcgatg
     1201 aaagataacc tcgaatttat taatacccac ccgaatctgg tcggattcct gatggggtta
     1261 ttaatttcga tggaagaaaa aggagaaaac cgcgacacca ttaaaggcct caaagtggca
     1321 ctgtttggcc caatcgccgg gattggcgat gcgattttct ggtttacttt gttgccgatt
     1381 atggcgggaa tttgctcatc atttgccagc ctgggaaacc tgctggggcc gattctattt
     1441 ttcgccgttt acctgcttat ctttttcctg cgcgtcggct ggacccacgt cggttattca
     1501 gtcggcgtga aggcgatcga taaagtgcga gagaactcgc agatgattgc tcgctcggca
     1561 accatcctcg ggatcacggt aatcggcggg ctgatcgctt cgtatgtgca tattaacgtg
     1621 gtgacatcgt ttgccatcga cagtacccac agcgtcgcgc tgcaacagga tttcttcgat
     1681 aaagtcttcc cgaacatttt accgatggcc tacaccctgc tgatgtatta cttcctgcgg
     1741 gtgaaaaaag cgcatccggt gctgttaatc ggcgtgactt ttgtgctctc tattgtttgt
     1801 tccgcattcg gcattttgta aatggaacga ggcactgctt ctggcggtgc ctctttactt
     1861 aaggaatttc atcctgtgca aacccttcag caagttgaaa actatacggc gttaagtgaa
     1921 cgagccagcg aatatttatt ggctgtgatc cgtagcaaac cggatgccgt gatttgcctg
     1981 gcgaccggag ccacgccatt actgacgtat cattatctgg tagaaaaaat ccaccagcag
     2041 caggttgata tcagccagct caccttcgtg aagctcgacg aatgggtgga tctgccatta
     2101 acgatgcctg gcacctgcga aactttccta caacagcata tcgtgcagcc gctggggcta
     2161 cgtgaagacc agctcatcag ctttcgctcc gaagagataa atgagacaga gtgcgaacgg
     2221 gtaacgaacc tgattgcgcg caaaggcggt ctggatttat gcgttctcgg attggggaaa
     2281 aacggtcatc ttgggctgaa cgaaccggga gaaagcctac aaccggcctg ccatatcagt
     2341 caacttgatg ccagaacaca gcaacatgag atgttaaaaa ccacgggtcg ccccgtgact
     2401 cgtgggatca ccttaggcct gaaggatatt ctcaatgccc gcgaggtttt gttattggtg
     2461 actggcgaag gaaagcagga tgcgacagaa cgttttctca cggctaaagt ctccaccgct
     2521 atcccggctt catttttatg gctgcacagt aactttattt gtttaattaa tacctaacca
     2581 gatgaaaaat ttgctcaaaa tttttaaatt aacttattca acagtcacgc attatattaa
     2641 ataacatttg actgggttga acataacgcc aatagcaaaa ggagttatgt tcaacaattt
     2701 aattttagat atacatataa cttgttctat taataaatca cctcgtattc tgactcggcc
     2761 cctggcgcag caaagtcagg catttatact c
//
LOCUS       DAZXUK010000367         2778 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZXUK010000367 DAZXUK010000000
VERSION     DAZXUK010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.369"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            2..541
                     /locus_tag="ACH3L8_004045"
     CDS             2..541
                     /locus_tag="ACH3L8_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFT4086918.1"
                     /translation="MYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSPGSLPL
                     TTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLG
                     WIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKL
                     AAKQHYKNAERLLAGLPAH"
     gene            complement(551..1978)
                     /locus_tag="ACH3L8_004046"
     CDS             complement(551..1978)
                     /locus_tag="ACH3L8_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFT4086919.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1975..2682)
                     /locus_tag="ACH3L8_004047"
     CDS             complement(1975..2682)
                     /locus_tag="ACH3L8_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFT4086920.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
BASE COUNT          795 a          664 c          612 g          707 t
ORIGIN      
        1 aatgtatata ttcgcaattt ttatagttgc agcaattacc tgtattagcc aacccaagaa
       61 aacgatgttg cgtgataaag ccatggtgaa ttatgccttt gattatttaa gatcaccagg
      121 cagtctaccg ttaaccacgg cagccacgga gctttccgcg attcatggtc actcaacatc
      181 gcaatatcgc cttggcgaat tttatcttca tggtagcgac ggtaaaccac tggattatac
      241 acaggcgaga tactggtatg agcaatcagc agaacaggaa aatccgcgcg cacaaagcaa
      301 actggggtgg atctacctga aaggcctggg agtcaaaccc gatacccgta aagcaattct
      361 ctggtataag gaagcagctg aacaagggta tgctcatgct caatatactt tgggtttgat
      421 ctacagaaat ggcactggta ttaatgttaa ccattatgaa tctcaaaaat ggttaaaact
      481 ggccgccaaa caacattaca aaaatgcgga aagattactt gccggacttc ccgcacatta
      541 acgtcgactg tcacccgaag agatggagta ttttccctat caaaccggca aagataaaaa
      601 tgtagaaaat aattttgatg acgcccagac agcccagccc gccatcatca acgggcattg
      661 gctgcggttg cggctccggc tcgacggttt cctgaccggt aaaattcgcc gcctgaatta
      721 ataatcgacg aataattaat gcttccagtg gacaacgatc ttctgcatca aggcggctca
      781 caatcggcat taatgattct tctgtcggtt ggctggcctg cactaattgt ttggcaacta
      841 aatataatgg ccataaggga tgttctgtat cacgcgtgag tgagatccgc ggcttacgtc
      901 ctttgtgcaa aaccatctcg accagtgaac gccccagcgc agagagtttt tcatcgctga
      961 gtatttcgta taaatgaaaa cgcgtacggg cgctgtcgcc ggggacataa agtagcggtt
     1021 ttccctgatc gttaaccaga tcacgcgctt caatcatcat caccactaat gggtaataat
     1081 agacccgatg ctgacggcac cattgcaaca ttaaagcatc cgcctgggtg gagggatact
     1141 cgcgccagta gtaacaaagt tctgccagca ggctttcgcc ttctccacaa tagacgcgtt
     1201 gtgcgtattc ataataacgc gcatcttcat tatcaggttc agcttcccgc cacgaccggg
     1261 cgtaatccag gagttcgggg attcccaggc ggcttgcgct ataccagcgg agtaaatttc
     1321 tgtgcagttt tgcatcatcg gggataatca ctgcgcgatg ttgcgctaaa tacaatgtca
     1381 gccattctgg atgcgatgac caaatccgag cgaacatttg gtaaaaatcg atggtgtcat
     1441 tttgcacggc aactggcaga tgcagaatgg tctggaagtt aaaaacattc cccttcgcaa
     1501 tggcatataa aaaggattcc agttcttctt cgtctatttc cccgacgttt tcctcctgca
     1561 accaccgcaa tctttctgct aataacacca cgcactcgaa ggataaatgg gcagtgttca
     1621 tggcgattgt gcacagcgac cagcgtaatt catcaatctc ttccatcgag caataattta
     1681 attgctgaat gaatcgctgc caggcggagg gcagaaaacg ttcgctatca gaggcaagta
     1741 acgcgcgaaa ggcgagcaga atttcatgtt ctgctacctc atattcttct ggttgccaaa
     1801 cagatttagc aggcgactgc gcaatccgta gcgcctcctc atacgcctga cgaagttgtt
     1861 taaaaccctg cggatcggtt tctggatgaa aggacggtaa cagcgccaga taagcgcggc
     1921 ggataatatc gacgtcagtc gtttcctcta tatcgaggat cttccagcaa tttttcataa
     1981 gtcgctcata tccttaagcg tttccgggca tgtggggtag tcagccagtg gttgccagga
     2041 caaggtaggg taatacgcat catcactggc aaataattga taaaacatat cattgccgga
     2101 atcgtcgtat tctttacgcg ctaagaattc gcgtaataaa ggcagatgat catcttcggg
     2161 agactgccaa tattgccgac caaaggtata accggcaaag tattgccgcc agttactata
     2221 ataacgtaga gcccgtagat gaatacgcga ctggatccat agactttctt cttcactcaa
     2281 ccagttatta agtacgccca ttcgacttaa aaaacccatc cggacataat cccatgcttt
     2341 aattcctccc tcaccacagc attcggctgt gctggcgaca aattgcgcat aagcctgacc
     2401 ttgctcattt aattcagcaa gatagtcacg ccattcacat ggagaataac gaaaccagcg
     2461 gtgataaaac cctgccaacc gagcggcatg accgttctcg atcattcggt gaattatgcg
     2521 cagcaggtca tcacggtcat caattcccca tgaggaagaa aggtcaaaaa agcatgtacc
     2581 taaatacatt ttgggggatg aatgcgaagg taaacagcat tcattgcgta tttccatcgg
     2641 agtggaaagt gcaaacaata ttttttgcga ccccatgtcc atataattta tccttaaaca
     2701 ttcatttaat gaatgtaaac tacatttcga ggctcagtta tttatacttt tctgacgcaa
     2761 cataatacgc ataaaata
//
LOCUS       DAZXUK010000368         2765 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZXUK010000368 DAZXUK010000000
VERSION     DAZXUK010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.370"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            241..1407
                     /gene="nhaA"
                     /locus_tag="ACH3L8_004048"
     CDS             241..1407
                     /gene="nhaA"
                     /locus_tag="ACH3L8_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFT4086921.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1473..2372
                     /gene="nhaR"
                     /locus_tag="ACH3L8_004049"
     CDS             1473..2372
                     /gene="nhaR"
                     /locus_tag="ACH3L8_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFT4086922.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2410..>2765)
                     /locus_tag="ACH3L8_004050"
     CDS             complement(2410..>2765)
                     /locus_tag="ACH3L8_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFT4086923.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          643 a          612 c          700 g          810 t
ORIGIN      
        1 ttaattatta cttttcctaa ttaatccctc aggaatcctc accttaagct atgattatct
       61 gggcttaggg tcactcgtga gcgcttacag ccgtcaaaaa cgcatctcac cgctgatggc
      121 gcaaattctt caatggctcg taaaaaacga attattccta cactataatc tgattttaac
      181 gatgattcgt gcggggtaaa atagtaaaaa cgatctattc acctgaaaga gaaataaaaa
      241 gtgaaacatc tgcatcgatt ctttagcagt gatgcctcgg gaggcattat tcttatcatt
      301 gccgctatcc tggcgatgat tatggccaac agcggcgcaa ccagtggatg gtatcacgac
      361 tttctggaga cgccggttca gctccgggtt ggttcactcg aaatcaacaa aaacatgctg
      421 ttatggataa atgatgcgct gatggcggta tttttcctgt tagtcggtct ggaagttaaa
      481 cgtgaactga tgcaaggatc gctagccagc ttacgccagg ccgcatttcc agttatcgcc
      541 gctattggtg ggatgattgt gccggcatta ctctatctgg cttttaacta tgccgatccg
      601 attacccgcg aagggtgggc gatcccggcg gctactgaca ttgcttttgc acttggtgta
      661 ctggcgctgt tgggaagtcg tgttccgtta gtgctgaaga tctttttgat ggctctggct
      721 attatcgacg atcttggggc catcattatc atcgcattgt tctacactaa tgacttatcg
      781 atggcctccc ttggcgtcgc ggctgtagca attgcggtac tcgcggtatt gaatctgtgt
      841 ggtgtacgcc gcacgggcgt ctatattctt gttggcgtgg tgttgtggac tgcggtgttg
      901 aaatcggggg ttcacgcaac tctggcgggg gtaattgtcg gcttctttat tcctttgaaa
      961 gagaagcatg ggcgttctcc agcgaagcga ctggagcatg tgttgcaccc gtgggtggcg
     1021 tatctgattt tgccgctgtt tgcatttgct aatgctggcg tttcactgca aggcgtcacg
     1081 ctggatggct tgacctccat tctgccattg gggatcatcg ctggcttgct gattggcaaa
     1141 ccgctgggga ttagtctgtt ctgctggttg gcgctgcgtt tgaaactggc gcatctgcct
     1201 gagggaacga cttatcagca aattatggtg gtggggatcc tgtgcggtat cggttttact
     1261 atgtctatct ttattgccag cctggccttt ggtagcgtag atccagaact gattaactgg
     1321 gcgaaactcg gtatcctggt cggttctatc tcttcggcgg taattggata cagctggtta
     1381 cgcgttcgtt tgcgtccatc agtttgacag gacggtttac cggggagcca taaacggctc
     1441 ccttttcatt gttatcaggg agagaaatga gcatgtctca tatcaattac aaccacttgt
     1501 attacttctg gcatgtctat aaagaaggtt ccgtggttgg cgcagcggag gcgctttatt
     1561 taactccaca aaccattacc ggacagattc gagcgctgga agagcgcctg caaggcaaat
     1621 tatttaaacg caagggacgt ggtctcgaac ccagcgagct gggagaactg gtctatcgct
     1681 atgccgataa aatgttcacc ttaagccagg aaatgctgga tattgtgaac tatcgcaaag
     1741 aatccaattt attgtttgac gttggcgtgg ctgatgcact ttccaaacgc ctggtcagta
     1801 gcgtacttaa cgccgcagtg gtagaaggcg agcccattca tcttcgctgc ttcgaatcca
     1861 cccacgaaat gctgctggag caattaagtc agcataaact ggatatgatc atttctgact
     1921 gtccgataga ctctacgcag caggaaggcc tgttctccgt gagaattggc gaatgtggcg
     1981 tgagtttctg gtgtacaaat ccaccaccag aaaaaccgtt cccggcttgt ctggaagaac
     2041 ggcgactttt gattcctggg cgacgttcaa tgttagggcg caaattgctt aactggttta
     2101 actcccaggg attaaacgta gaaatcctcg gcgagtttga tgatgccgct ttgatgaaag
     2161 cttttggtgc gatgcacaat gcaatcttcg ttgccccaac gctttatgca tatgactttt
     2221 atgccgataa aactgtcgta gaaattggtc gcgtcgagaa tgtgatggaa gagtaccatg
     2281 ctatttttgc tgagcggatg attcagcacc cggcggtaca gcgaatctgc aatacggatt
     2341 attctgcgct ttttagtcca gcggtgcgtt aatcggcagc tcccccaaag ttaaggtggg
     2401 ggagatagat tagttgtaca ttaccacgat tttgactcgg ctcattattt gcccgcttga
     2461 gacattgttt ccatatgtac gcgggcgaat aaatagagga atctgattac ttccttcatg
     2521 gggatgctga aaagagtagt aattgctttt accatactgg atttcattcg taccatcatt
     2581 ggagagttga aagcctattt gacccgcacc gtcctcactg ttttctaact cattttttaa
     2641 tatgctggtt cctgactcct gtatcgtcac tggttcaaag taatattcaa cgtcactggg
     2701 ttcaaaaact aattcacctt gctcgccctg gatgcagtca aatcttatat gatgaaactg
     2761 ataat
//
LOCUS       DAZXUK010000369         2736 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZXUK010000369 DAZXUK010000000
VERSION     DAZXUK010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.371"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1603
                     /gene="mppA"
                     /locus_tag="ACH3L8_004051"
     CDS             <1..1603
                     /gene="mppA"
                     /locus_tag="ACH3L8_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFT4086924.1"
                     /translation="VSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDEPASL
                     DPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNAKWAD
                     GTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTAVDAH
                     TLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERVVNEK
                     LVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKLLKDI
                     PGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPAWHFI
                     PDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQKIAI
                     AVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLLTSMH
                     SGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNGRLIK
                     PWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1654..2685)
                     /gene="ynaI"
                     /locus_tag="ACH3L8_004052"
     CDS             complement(1654..2685)
                     /gene="ynaI"
                     /locus_tag="ACH3L8_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFT4086925.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT          791 a          623 c          646 g          676 t
ORIGIN      
        1 tgtttcagtt acgtgttgtg cgctgttggt cagcagcatt tctctttcgt atgctgcaga
       61 agttccgagc ggcacagtac tggcagagaa gcaggagctg gtgcgccaca ttaaagatga
      121 gcctgcgtcg ctggatcccg ctaaagccgt gggcctgcca gagattcagg tcattcgcga
      181 tctgtttgaa ggtctggtga atcagaacga aaaaggggag attgtccccg gcgtcgcgac
      241 tcaatggaaa agtaatgaca accgtatctg gacttttacc ctgcgcgata acgcaaaatg
      301 ggcggatggc acaccggtaa cggcgcaaga ttttgtctac agctggcaac gtctggtgga
      361 cccaaaaaca ttgtcgccat ttgcatggtt tgccgcgctg gcgggaatca acaacgcaca
      421 ggcgattatt gatggtaaag ctacgcctga tcagcttggc gtcaccgcag ttgatgccca
      481 tactttgaaa attcagcttg ataaaccgtt gccgtggttt gtgaatttaa ccgctaactt
      541 tgccttcttc ccggtgcaaa aagccaacgt agaaagcggt aaagagtgga cgaaacccgg
      601 aaatctgatc ggcaatggcg cttatgttct taaagagcgc gtagtcaatg aaaaactggt
      661 cgtggtaccg aatacccatt attgggataa cgccaaaacg gtgctgcaaa aagtgacctt
      721 cctgccaatt aatcaggaat ccgcagccac taagcgttac ctcgcggggg atattgatat
      781 caccgaatcc ttcccgaaaa atatgtatca gaagctgttg aaggatattc cggggcaggt
      841 ttatacgcca ccgcagctcg ggacctatta ttatgccttt aacacgcaaa aagggccgac
      901 ggcagatcag cgcgttcgcc tggcattaag tatgacgata gatcgccgcc tgatgaccga
      961 aaaagtgttg gggacgggcg aaaagccagc gtggcatttt ataccagatg ttaccgccgg
     1021 atttacgccg gaaccttcgc cgtttgaaca aatgagtcag gaagaactga atgcgcaggc
     1081 aaaaactttg ttgagcgcag ctggttatgg tccgcaaaaa ccgctgaagc tgacgctttt
     1141 gtataacact tcagaaaacc atcaaaaaat tgcgattgct gtagcatcga tgtggaaaaa
     1201 gaaccttggc gtagatgtta aattgcaaaa tcaggaatgg aaaacctata tcgatagccg
     1261 taacaccggc aattttgatg ttatccgcgc ctcatgggtg ggggattata atgaaccctc
     1321 cactttcctg acattattaa cgtcaatgca ttcaggaaat atttcacgct ttaacaatcc
     1381 ggcatacgac aaagttctgg cccaggcatc gacggaaaat accgttaaag cgcgtaatgc
     1441 cgattacaac gcggcagaaa aaatcctcat ggagcaagca ccgattgcac caatttatca
     1501 atataccaat ggacgattaa tcaaaccgtg gctgaaaggt tatcccatta ataaccctga
     1561 agatgtagcg tacagtcgga ctatgtatat tgtgaagcat tgatgtgatg ggaactggcg
     1621 ttacccttgt ggataacgcc agtgatgtct gatttagcgc ccctgttccg gcggtgtaat
     1681 gttatccata tacagcgtct ggctggggaa ggcaaagtcc gcgccgtgtg actgtacaat
     1741 atcgataatc ttcaaataaa cgtcttgctg tgcagcaagc cactcagccc ataccgtggt
     1801 tttggtaaag caataaacca taatattcaa tgacgagtca gcaaactggt tgaaataaac
     1861 cagtaaggtt tgtcgctggt cgatggccgg gtgatttttc agcatctcac gtacggcttc
     1921 gacaataacg cccacttttg ccgcatcctc ataacgtaaa ccaatggtcg tggtaatgcg
     1981 gcggttggtc attcgtcctg ggttttctac gctgatcgac gaaaacagcg agttcggtac
     2041 gtacaatgga cgattatcaa aggtcgtaat tttggtaatt cgccagccaa tttccgctac
     2101 tgtaccttcg atatttctgt ccggtgaacg gatccagtcg ccaatactga aaggacggtc
     2161 gaaatagagc atgatcccgg aaaagaaatt actcagaata tctttaccgg ccataccgac
     2221 agccagacca ccaataccac caaaggtcag caagccagaa aggctcatgc cgaaatgttc
     2281 gccataaagc agaacaagca ctacaataat ggtgattttg ataatgcgcg acataatccg
     2341 cgcactggtg atatcgcgac cttttttaat ctgctgtttt tcaaactgat taatcagcag
     2401 aaatagctta atcgtcagaa taaccgcaat cagggacgta cagataaaat cgataacgcc
     2461 tggggtgata aatttgagtt tatagttttc tataacataa ttactgatgc taccaacagc
     2521 actgataatt atggtgtaga ttaaaaattg caccgcatgg aataaaaatc cttttctttt
     2581 acgatttcca cggcgaaacc aaaagctcat cagaatcaat gctgcgcagc taccgaaaat
     2641 aatgaccaga ttaagcgcat tatttgtaaa cagttcagcg atcattgttt tatcaggctc
     2701 ctccagataa ttgtcgtcat gccggaaacc cctggc
//
LOCUS       DAZXUK010000370         2732 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZXUK010000370 DAZXUK010000000
VERSION     DAZXUK010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.372"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..136
                     /locus_tag="ACH3L8_004053"
     CDS             <1..136
                     /locus_tag="ACH3L8_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001634432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HFT4086926.1"
                     /translation="APKHASWNSNRLMRNQKNRSIRAKPQLPRLLPAFRPKKPPSKRL
                     "
     gene            148..1206
                     /gene="rsxD"
                     /locus_tag="ACH3L8_004054"
     CDS             148..1206
                     /gene="rsxD"
                     /locus_tag="ACH3L8_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFT4086927.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            1210..1830
                     /gene="rsxG"
                     /locus_tag="ACH3L8_004055"
     CDS             1210..1830
                     /gene="rsxG"
                     /locus_tag="ACH3L8_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFT4086928.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            1834..2529
                     /gene="rsxE"
                     /locus_tag="ACH3L8_004056"
     CDS             1834..2529
                     /gene="rsxE"
                     /locus_tag="ACH3L8_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFT4086929.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            2529..>2732
                     /locus_tag="ACH3L8_004057"
     CDS             2529..>2732
                     /locus_tag="ACH3L8_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="DNA-(apurinic or apyrimidinic site) lyase; has
                     apurinic or apyrimidinic endonuclease activity and DNA
                     N-glycosylase activity; removed damaged DNA at cytosines,
                     thymines and guanines; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFT4086930.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELG"
BASE COUNT          620 a          709 c          740 g          663 t
ORIGIN      
        1 cgcgccaaag cacgcaagct ggaacagcaa caggctaatg cggaaccaga agaacaggtc
       61 gatccgcgca aagccgcagt tgccgcggct attgcccgcg ttcaggccaa aaaagccgcc
      121 cagcaaaagg ttgtaaacga ggactaaatg gtattcagaa tagctagctc cccttatacc
      181 cataaccagc gccagacatc gcgcattatg ctgttggtgt tgctcgcagc cgtgccagga
      241 atcgcagcgc aactgcggtt ttttggttgg ggtactctcg ttcagatcct gttggcgtcg
      301 gtcagtgctc tgttagccga agctctcgta ctcaaactac gcaagcagtc ggtagccgca
      361 acgttgaaag ataactcagc attgctgaca ggcttattgc tggcggtaag tattcccccc
      421 ctagcgccat ggtggatggt cgtactgggt acggtgtttg cggtgatcat cgctaaacag
      481 ttgtatggcg gtctggggca aaacccgttt aatccggcaa tgattggtta tgtggtctta
      541 ctgatctcct tcccggtgca gatgaccagc tggttaccgc cacatgaaat tgcggtcaac
      601 atccctggtt ttatcgacgc catacaggtt attttcagcg gtcataccgc cagtggtggt
      661 gatatgaata cgctacgctt aggtattgat ggcattagtc aggcgacacc gctggataca
      721 ttcaaaacct ctgtccgcgc aggtcattcg gttgaacaga ttatgcaata tccgatctac
      781 agcggtattc tggcgggcgc tggttggcaa tgggtaaatc tcgcctggct ggctggtggc
      841 gtgtggttgc tatggcagaa agcgattcgc tggcatattc ccctcagctt cttagtaacg
      901 ctggcgttat gcgcaacgtt gggctggttg ttctcaccag aaacactggc agcaccgcaa
      961 attcatctgc tgtctggtgc gactatgctc ggcgcattct ttattttgac tgacccagtt
     1021 actgcttcta cgaccaatcg tggtcggctg atgttcggcg cgctggcggg cttattagtc
     1081 tggttgatcc gcagtttcgg cggctatcct gacggcgtgg cttttgccgt cctgctggcg
     1141 aacatcacgg ttcctctgat cgattactac acgcgtccgc gcgtctacgg ccatcgcaaa
     1201 gggtaaacca tgctgaaaac tatccgaaaa cacggcatta cactggcact atttgcagcg
     1261 ggttctacag ggttaactgc ggccatcaac cagatgacca aaacgacaat tgctgaacag
     1321 gccagtctgc aacaaaagac gttatttgat caggtgctgc cagccgaacg ctataacaat
     1381 gcgctcgcac agagttgcta tctggtaact gcgccagagt taggtaaagg tgagcatcgg
     1441 gtttacatcg ccaaacagga tgacaaaccg gtagccgccg ttctggaagc aaccgcgcca
     1501 gatggctatt ccggtgcgat tcagctgctg gtgggagccg attttaatgg cacggtactt
     1561 ggcacgcgcg tgacagagca ccacgaaacg ccagggcttg gcgataaaat cgaattgcgc
     1621 ctttctgact ggctcaccca ttttgcggga aaaaaaatca gtggtgcgga tgatgcgcac
     1681 tgggcggtga agaaagatgg tggtgatttc gaccagttca ccggcgcgac gattactccc
     1741 cgcgcggtgg ttaatgcggt aaaacgcgcc ggattgtacg ctcagacgtt accggcacaa
     1801 ctttctcaac ttcctgcctg tggagaataa agcgtgagcg aaattaaaga cgttattgtt
     1861 caggggttgt ggaaaaacaa ctctgcactg gtccaactgc tcggcctttg tccgctattg
     1921 gcggtcacat ccactgccac taacgctctg ggtttaggac ttgcgactac gctggtactg
     1981 acgctgacca acctgaccat ttcgacgctg cgtcactgga cgccagccga gatccgcatt
     2041 cccatttacg tgatgatcat cgcctcggtg gtcagcgctg tacagatgct gatcaacgcc
     2101 tacgcctttg gcctgtatca atcattaggg atttttattc cgctgattgt cactaactgt
     2161 atcgttgtgg gccgcgctga agccttcgcc gccaaaaaag gtccggcgct ttcggcactg
     2221 gacggctttt caattggtac gggcgcaacc tgcgccatgt tcgtgctggg ttcactacgc
     2281 gaaattatcg gcaatggcac gttgtttgac ggtgcagatg cgctgttagg tagctgggca
     2341 aaagtattac gcgtggagat tttccacacc gactcccctt tcctgctggc gatgctgcca
     2401 ccaggtgcat ttattggctt gggactgatg ctggcaggaa aatacctgat tgatgaaaga
     2461 atgaaaaagc gccgtgctga agcagctgcc gaacgtgcat tgccaaacgg tgaaacaggg
     2521 aatgtctgat gaacaaagca aaacgtctgg agatcctcac tcgcctgcgt gagaacaatc
     2581 ctcatcccac caccgagctt aatttcagtt cgccttttga attgctgatt gccgtactgc
     2641 tttccgctca ggcgaccgat atcagtgtta ataaggcgac ggcgaaactc tacccggtgg
     2701 cgaatacgcc tgcagcgatg cttgaactag gc
//
LOCUS       DAZXUK010000371         2716 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZXUK010000371 DAZXUK010000000
VERSION     DAZXUK010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.373"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..805)
                     /locus_tag="ACH3L8_004058"
     CDS             complement(<1..805)
                     /locus_tag="ACH3L8_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFT4086931.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQR"
     gene            897..983
                     /locus_tag="ACH3L8_004059"
                     /pseudo
     CDS             897..983
                     /locus_tag="ACH3L8_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(993..1073)
                     /locus_tag="ACH3L8_004060"
                     /pseudo
     CDS             complement(993..1073)
                     /locus_tag="ACH3L8_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1388..1864)
                     /locus_tag="ACH3L8_004061"
     CDS             complement(1388..1864)
                     /locus_tag="ACH3L8_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFT4086932.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1923..>2716)
                     /locus_tag="ACH3L8_004062"
     CDS             complement(1923..>2716)
                     /locus_tag="ACH3L8_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFT4086933.1"
                     /translation="GYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVII
                     EPIVQGAGGMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPD
                     ILCLGKALTGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAI
                     IESGEWQQQVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFV
                     EQGVWIRPFGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
BASE COUNT          702 a          620 c          692 g          702 t
ORIGIN      
        1 atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata ttaacatctg
       61 cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg ttagggatat
      121 atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt tccccaacgt
      181 ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta tcaaaagcac
      241 ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg ttgccaacag
      301 aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg agaagggctg
      361 agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg ttaagtgaaa
      421 catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg ttatcgctgg
      481 ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca ttgctagaga
      541 tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg agtaaactta
      601 aattgagggc tggctcttga aaagccagac atattttcag taatcgaact gcctgtgttc
      661 ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga taatccgtga
      721 tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag gagcggaaaa
      781 aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata tattaatata
      841 ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt taagaacagg
      901 aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg ctcaatgtgc
      961 tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca gtagtctgcc
     1021 caccacagca tcatttctct gcgcttatcg agatactgag catggttgta aattcagcta
     1081 acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg cgctaatgct
     1141 ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga tatgttgtgt
     1201 tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat tgatattaat
     1261 gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg ggaaggtaaa
     1321 aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc gccatctggc
     1381 agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg aaatgaacgt
     1441 taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct acatccagcg
     1501 cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg ccatcgtatc
     1561 cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct accagaccag
     1621 agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact acccagtgcc
     1681 accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa cttttcgttc
     1741 ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg taacccatgc
     1801 cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta ctgatgagtt
     1861 tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac gctgacttcc
     1921 cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc ggtcagacgc
     1981 tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt gccaaaaggc
     2041 cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt caccggacga
     2101 gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat ttcggcatca
     2161 cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg ctgctgccat
     2221 tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag cggattgccc
     2281 ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt ttctgcaacc
     2341 tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc tttaccgagg
     2401 cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc ggtacgacca
     2461 aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca cattttgcgg
     2521 attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg gacaatcggc
     2581 tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc aaaaccaacc
     2641 atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc aaacaagttt
     2701 tctggcagat agcctt
//
LOCUS       DAZXUK010000372         2695 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZXUK010000372 DAZXUK010000000
VERSION     DAZXUK010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.374"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(121..1545)
                     /gene="patD"
                     /locus_tag="ACH3L8_004063"
     CDS             complement(121..1545)
                     /gene="patD"
                     /locus_tag="ACH3L8_004063"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFT4086934.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1567..2361)
                     /locus_tag="ACH3L8_004064"
     CDS             complement(1567..2361)
                     /locus_tag="ACH3L8_004064"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4086935.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2351..>2695)
                     /locus_tag="ACH3L8_004065"
     CDS             complement(2351..>2695)
                     /locus_tag="ACH3L8_004065"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4086936.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT          685 a          799 c          633 g          578 t
ORIGIN      
        1 tcaaacattt agcctaattt ttacgccagt tattaatgta atcattctaa tacttaaccg
       61 ccattaaact cagaacctaa aacgatagtg gccgtcctat aacagccact aacatctaac
      121 ttaatgttta accatgacgt ggcggacgac ggtgtaatcc tccagcccat aaagtgacat
      181 atccttgccg taaccagaaa gtttctgccc accgtgcggc atttcactta ccagcatgaa
      241 atgggtattg acccaggtac aaccatattg cagccgtgcg ctgacgcgat gcgccctgcc
      301 cacatctttc gtccataccg aagatgcaag tccgtactgg ctgtcattcg cccagttcac
      361 cacctgttct tcgttgtcga agggcgtaac actcactact ggaccaaata cctctttttg
      421 cacgatggca tcgtcctgta atgcgccagc cagcagcgtc ggcgcatagt aatagccatt
      481 acccttgcgc ttttcaccgc cagtgatcac tttgatgtgc cctgtcgctt tcgcctcttc
      541 tactgccttg ctgacgcgtt cgagatgcgc cagcgagctt aaaggtccaa gctccgtaga
      601 ctcgtcatcc ggcgcaccag attttaacgt tgccaccgca gcacccagtt tttccaccag
      661 cgtatcgtaa atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc
      721 attgtaatag ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa
      781 aacaatcact ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt
      841 atggctgatg atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt
      901 gggatgaccg gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac
      961 gcctgccggg aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat
     1021 ttctgatggt ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc
     1081 ggccatcatc agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg
     1141 acggatcatc gaagtatgac cttcaagata ttcacctgcc gccagaccat tcagacagcg
     1201 cgccgcaccc gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc
     1261 actatgcagc ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc
     1321 ttcgataaca tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc
     1381 ccattcagca aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatac
     1441 ctcggcaatc tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc
     1501 ttcgccgcta acccgttctc cgttaatcag taacttatgt tgcatagctt attcctgtat
     1561 cagtggttat tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa
     1621 ggttgttacc aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg
     1681 cccaagctga ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt
     1741 aacgatgatt tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga
     1801 actgagattc ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat
     1861 tgacgcctca accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac
     1921 caccactacg caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt
     1981 gatggtttta aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg
     2041 cagcagcaat aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc
     2101 agctgccagc gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga
     2161 cagcgtcacg gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt
     2221 caggccctgc ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat
     2281 tatcaggatg ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa
     2341 cggtgcgcgt tcagagtgca tcgaacgctc ccagacgttt cacgaacgcc aggtacagtg
     2401 cgatgagaat aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc
     2461 cctgctggga ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga
     2521 cgataaaatc gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga
     2581 ttgccagcgg cagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg
     2641 acgcctgcaa caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggat
//
LOCUS       DAZXUK010000373         2680 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZXUK010000373 DAZXUK010000000
VERSION     DAZXUK010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.375"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            82..1056
                     /gene="cysB"
                     /locus_tag="ACH3L8_004066"
     CDS             82..1056
                     /gene="cysB"
                     /locus_tag="ACH3L8_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFT4086937.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1375..1515
                     /locus_tag="ACH3L8_004067"
     CDS             1375..1515
                     /locus_tag="ACH3L8_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFT4086938.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            1518..1685
                     /gene="yciX"
                     /locus_tag="ACH3L8_004068"
     CDS             1518..1685
                     /gene="yciX"
                     /locus_tag="ACH3L8_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFT4086939.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1799..1894
                     /gene="ymiC"
                     /locus_tag="ACH3L8_004069"
     CDS             1799..1894
                     /gene="ymiC"
                     /locus_tag="ACH3L8_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFT4086940.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2058..>2680
                     /locus_tag="ACH3L8_004070"
     CDS             2058..>2680
                     /locus_tag="ACH3L8_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFT4086941.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTL"
BASE COUNT          696 a          620 c          631 g          733 t
ORIGIN      
        1 tttattatta aatcgtatta gtcacccgcc aggtgtgacg aaaaaacgat gttctgatgg
       61 cgtctaagtg gatggtttaa catgaaatta caacaacttc gctatattgt tgaggtggtc
      121 aatcataacc tgaatgtctc atcaacagcg gaaggacttt acacatcaca acccgggatc
      181 agtaaacaag tcagaatgct ggaagacgag ctaggcattc aaattttttc ccgaagcggc
      241 aagcacctga cgcaggtaac gccagcagga caagaaataa ttcgtatcgc tcgcgaagtc
      301 ctgtcgaaag tcgatgccat aaaatcggtt gccggtgagc acacctggcc ggataaaggc
      361 tcgctgtata tcgccaccac gcatacccag gcacgctacg cgttaccaaa cgttatcaaa
      421 ggctttattg agcgttatcc ccgcgtttct ttgcatatgc accagggccc gccgacacaa
      481 attgctgatg ccgtctctaa aggcaatgcc gatttcgcta tcgccacaga agcgctgcat
      541 ctgtatgaag atttagtgat gttaccgtgc taccactgga atcgggctat tgtagtcact
      601 ccggatcacc cgctggcagg caaaaaagcc attaccattg aagaactggc gcaatatccg
      661 ttggtgacat ataccttcgg ctttaccgga cgttcagaac tggatactgc ctttaaccgc
      721 gcagggttaa cgccgcgtat cgttttcacg gcaacggatg ctgacgtcat taaaacttac
      781 gtccggttag ggctgggggt aggggtcatt gccagcatgg cggtggatcc ggtcgccgat
      841 cccgaccttg tgcgcgttga tgctcacgat atcttcagcc acagtacaac caaaattggt
      901 tttcgccgta gtactttttt gcgcagttat atgtatgatt tcattcagcg ttttgcaccg
      961 catttaacgc gtgatgtcgt tgatgcggct gtcgcattgc gctccaatga agaaattgag
     1021 gtcatgttta aagatataaa actgccggaa aaataatttc ttgcgttatt ttcggcacct
     1081 tttatgtagc gaaggtgccg gaatatattc tcttttgtta cttatttcta taccgtgcac
     1141 aatattttta tccctcatat ttatagggta aattacctgt aaagtagtgt ctaattaccc
     1201 gcctcgcaaa ttccagtttt caaatatcta tttcaggtca aaagattgaa ttattcactg
     1261 gagacgattc gtaaattcgc tggatttttg actaaagttt ctttaggatt tgtctcatcg
     1321 tatgattaat tacaccaatc attcaacgtc ttgctgataa gtgatggcga ttatatgagg
     1381 ttagcaatgc cttctggaaa tcaggaaccc cgccgtgatc ctgaattaaa acgtaaagcc
     1441 tggctggcgg tttttcttgg ttctgcactt ttctgggtgg ttgtcgcact gctgatttgg
     1501 aaagtgtggg gataactatg gtcggtcagg agcaactgga gtcgtcacca ttatgccagc
     1561 atagtgacaa tgaaccagaa acgaaacggg aatgttccgt cgttattcca gacgactggc
     1621 aactaacatc gcagcagcaa gcctttatag aactgtttgc tgaagatgat cagccgaaac
     1681 aataattatc atcattctta ttacccattt ttaatgaatt aaagggcttt taatacaccg
     1741 cagcaataac agcttgagtt atctcaacac aaaataataa ccgttaaggg tgtagcctat
     1801 gatcaacaca aatatgaaat attggtcctg gatgggcgcg ttttctctgt cgatgctctt
     1861 ctgggccgaa ctcctctgga tcattactca ctgatccttg accccgctgc ggcggggttg
     1921 tcatttgctt tgccacaagg tttctcctct tttatcaatt tgggttgtta tcaaatcgtt
     1981 acgcgatgtt tgtgttatct ttaatattca ccctgaagag aatcagggct tcgcaaccct
     2041 gtcattaagg aggagctatg tcgtcaaccc tacgagaagc cagtaaggac acgttgcagg
     2101 ccaaagataa aacttaccac tactacagcc tgccgcttgc tgctaaatca ctgggcgata
     2161 tcacccgtct acccaagtca ctcaaagttt tgctcgaaaa cctgctgcgc tggcaggatg
     2221 gtaactcagt taccgaagag gatatccacg cactggcggg atggctgaaa aatgcccatg
     2281 ctgaccgtga aattgcctac cgcccggcaa gggtgctgat gcaggacttt accggcgtac
     2341 ctgccgttgt tgatctggcg gcaatgcgcg aagcggttaa acgcctcggt ggcgatactg
     2401 caaaggttaa cccgctctca ccggtcgacc tggtcattga ccactcggtg accgtcgatc
     2461 gttttggtga tgatgaggca tttgaagaaa acgtacgcct ggaaatggag cgcaaccacg
     2521 aacgttatgt gttcctgaaa tggggaaagc aagcgttcag tcggtttagc gtcgtgccgc
     2581 caggcacagg catttgccat caggttaacc tcgaatatct cggcaaagca gtgtggagcg
     2641 aattgcagga cggtgaatgg attgcttatc cggatacact
//
LOCUS       DAZXUK010000374         2666 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZXUK010000374 DAZXUK010000000
VERSION     DAZXUK010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.376"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            514..657
                     /locus_tag="ACH3L8_004071"
     CDS             514..657
                     /locus_tag="ACH3L8_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFT4086942.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            670..879
                     /gene="cspE"
                     /locus_tag="ACH3L8_004072"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             670..879
                     /gene="cspE"
                     /locus_tag="ACH3L8_004072"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFT4086943.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            1045..1854
                     /gene="rlmA"
                     /locus_tag="ACH3L8_004073"
     CDS             1045..1854
                     /gene="rlmA"
                     /locus_tag="ACH3L8_004073"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFT4086944.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1851..2417)
                     /gene="mntP"
                     /locus_tag="ACH3L8_004074"
     CDS             complement(1851..2417)
                     /gene="mntP"
                     /locus_tag="ACH3L8_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFT4086945.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     regulatory      complement(2549..2639)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
BASE COUNT          700 a          623 c          634 g          709 t
ORIGIN      
        1 atattttcct cattaattta ttaggatgtt tacatcagat ttgtgattaa gcgtggtatt
       61 atttattagc gaaacgtttc tctcttgatt tttttgctca ctcatcaatt tttcttattt
      121 taaatttaca atccctttgg ggattgactt ctctttaggg taattgatag ccgttaactg
      181 actgttttat gagaaaaagt gatataactt tttattcatt gcatagcaga aaatgtgata
      241 ttgcacgcac tatgtaataa cttctccctc tggcctggaa caactgaact tattgaacta
      301 tgttagaaac tacgccagtt taagtatctg cctgaactgg caaggttaag cacaatgata
      361 tatcggcgcg tattccgttg cataagtgtg caaaaaaagt ggaagacgta tcgagatttg
      421 tgcgtctgat cgagatatgt ttaaaaatgg cttgccataa ttaacgttgt atgtgataaa
      481 agatttcggg ttaaacgagg tacagttctg tttatgtgtg gcattttcag taaagaagtc
      541 ctgagtaaac acgttgacgt tgaataccgc ttctctgccg agccttatat tggtgcctca
      601 tgcagtaatg tgtcagtttt atctatgtta tgcctgcggg cgaagaaaac aatctaagga
      661 atttttcaaa tggcaaagat taaaggtcag gttaagtggt tcaacgagtc taaaggtttt
      721 ggcttcatta ctccggctga tggcagcaaa gatgtgttcg tacacttctc cgctatccag
      781 ggtaatggct tcaaaactct ggctgaaggt cagaacgttg agttcgaaat tcaggacggc
      841 cagaaaggtc cggcagctgt taacgtaaca gctatctgat cgaatccact gatctgaagt
      901 gtgaatacgc ttcaatctcg ctataaagcc tcgtcgaatg cgaggctttt tactatgctt
      961 tatcttcgct cctggcgttc ggatatttgc ccgccgcgtg attcgcgtta cacttgcggc
     1021 ctttagtatc ctgccggagt tgtcatgtcg ttttcctgtc cactttgcca tcagcctctt
     1081 tcgcgtgaaa aaaacagcta tatctgtccc caacgacatc agtttgatat ggcgaaagaa
     1141 gggtatgtca atctgctgcc cgttcagcat aaacggtctc gtgatccggg cgacagcgcg
     1201 gaaatgatgc aagcacgccg cgcattctta gatgccggac attatcagcc gctgcgtgat
     1261 gcaattgtcg cccaactgcg ggaacggctt gatgaaaagg ccacggcggt gctggatatt
     1321 ggctgtggtg aagggtatta cacacacgca tttgccgatg cgttgcccga aatcaccacg
     1381 tttggcctgg atgtctcgaa ggtagcgata aaagcggcgg cgaaacgcta tccgcaggtc
     1441 actctttgtg tcgcttccag ccaccgtttg ccgttttccg ataccagtat ggacgccata
     1501 atacgtattt acgcgccgtg taaagcagaa gaattagcac gagtagtgaa gcccggcggc
     1561 tgggtcatta ctgccacgcc gggaccgcga catttgatgg agctgaaggg gctgatttac
     1621 aatgaagtac atcttcatgc acctcatgca gaacaactgg aaggttttac attacagcag
     1681 agtgcggagt tgtgttatcc gatgcgtctt cgcggtgatg aagccgtcgc attattgcag
     1741 atgacgccgt ttgcctggcg tgcgaagcca gaagtctggc aaacactggc agcaaaagaa
     1801 gtgttcgact gccagacgga ctttaatatt cacctctggc agcgttctta ttaaccgtgg
     1861 aagtgcgtcc agaggatctg gacgccgatg ccgatcagca ccagcccgcc gagaatttcc
     1921 gcttttttcc caataattga gccgataaag cgaccaacca tcatccctaa tgtcgacata
     1981 atcaaggttg cacaaccaat ggccaatgcg gtcgcgataa tgttgacctg aaggaaagca
     2041 agaccaacac ccacagccat ggcatccagg ctggtggcaa tcgcggtggt taccagtagc
     2101 cagaaaccgt gccgacggcg cggctcttca tcttcatcat ctgcgccacg aaaaccctca
     2161 ataatcattc gcccgccgag gaatatcagc agcacaaacg caatccagtg attccattca
     2221 aggacaaacc ggctggctaa catgcccatt ccccagccga tcagcggcgt cagggtttcg
     2281 acggcaccaa aaataaggcc ggttcgcaat gcttcagaaa atttcggttt atggaggggg
     2341 gcacctttac cgattgatgc agcaaatgca tccatcgaca taccaaacgc aagaagaaca
     2401 gtagcagtga tattcatgac aacgtcctga ccggggtatc cataaaacac atcatcgccc
     2461 ccagcaaaca tgcatacatg aacaacaagc cgcgatgggt tttcgcgttc atgcaggcat
     2521 agcgattgat gtgcatatgg tctcgcctga ctgaaagtct tcagtccgta cgtgccacgt
     2581 tttgcaacga gtatgttgac acgtacattt ccggaatctg gaaatcggct actccccaaa
     2641 tgagggcgca accttaacat attttg
//
LOCUS       DAZXUK010000375         2658 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZXUK010000375 DAZXUK010000000
VERSION     DAZXUK010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.377"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..817
                     /locus_tag="ACH3L8_004075"
                     /pseudo
     CDS             1..817
                     /locus_tag="ACH3L8_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
     gene            860..2263
                     /locus_tag="ACH3L8_004076"
     CDS             860..2263
                     /locus_tag="ACH3L8_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4086946.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            2331..>2658
                     /locus_tag="ACH3L8_004077"
     CDS             2331..>2658
                     /locus_tag="ACH3L8_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFT4086947.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT          614 a          614 c          647 g          783 t
ORIGIN      
        1 cagaaacttg gctatatctt cgccgccacg ctgttttcac tgttcggcct gctgtttatg
       61 tggatctgct actcaggcgt gaaagagcgt tatgtcgaaa cccaaccaac caatccggcg
      121 caaaagcctg gcctgttgca atctttccgc gcaattgccg gtaaccgccc actgttcatt
      181 ctgtgtattg ccaacctctg caccttaggg gcgtttaacg tcaagctcgc cattcaggtc
      241 tattacaccc agtacgtact taacgatccc atcctgttgt cgtatatggg atttttcagc
      301 atgggctgta ttttcatcgg cgtgttcctg atgcctggcg cagtcagacg ttttggtaag
      361 aagaaggtct atatcggcgg cctgctgatt tgggtgctgg gcgatctgct caactatttc
      421 ttcggcggcg gctcggtcag ctttgtggcg ttctcctgcc tggcgttctt cggctcagcg
      481 cctgaactgg gcgctggttt ccgacaccgt cgagtacggc gagtggcgca ccggcgtgcg
      541 ttcggaaggc acggtctaca ccggcttcac cttctttcgc aaagtgtctc aggcgctggc
      601 tggtttcttc cccggctgga tgctgacgca aattggctat gtgccgaacg tcgcacaggc
      661 tgaccacacc attgaagggt tacgccagtt gatcttcatc tacccaagcg cactggcggt
      721 agtcaccatt gtggcgatgg gttgcttcta cagcctgaac gagaagatgt atgtccgcat
      781 tgtggaagag atagaagccc gtaaacgcac ggcgtaataa taataaacga cgccctgcgg
      841 ggcgttataa ggagtgatta tgtctgacca taatccactg acattaaaac tgaatctgcg
      901 ggaaaaaatc gcctatggta tgggcgacgt cggttcgaat ttaatgctct gcatcggtac
      961 tctgtatctc ctcaaacttt ataccgatga attagggatg cctgcttact atggcgggat
     1021 tatctttctg gtcgcgaagt tttttaccgc gtttaccgat atgctcaccg gatttttact
     1081 cgactcgcgg aaaaatattg ggcctaaagg caaatttcgt ccctttattt tatatgcagc
     1141 agttccggcg gcgttaattg caacgctcca gtttatcgcc accacctttt gtttgccggt
     1201 taaaacgacg attgccacca cgctgtttat gatgtttgga ctttcataca gtctgatgaa
     1261 ctgctcgtat ggtgcgatga tcccggcaat taccaaaaac ccgaatgagc gcgcgcagct
     1321 cgcggcttac cgccagggtg gtgcaaccat agggctattg atttgtaccg tggcgtttat
     1381 tccgttgcag tcgctttttt ctgactcaac cgtcggttat gcctgtgcgg cacttatgtt
     1441 ctccattggc ggctttattt ttatgatgct gtgctacaga ggcgtcaaag agcattatgt
     1501 ggacacagcg ccaaccggac ataaagccag tattctcaaa tctttttgcg cgatatttcg
     1561 taatccacca ttgctggttt tatgcattgc taacctgtgt actctggcgg catttaatat
     1621 caaactggcg attcaggtct attacaccca gtatgtgctg aatgatatta atttattgtc
     1681 gtggatggga ttcttcagta tgggatgcat cctcgtcggc gtattactgg tgccagtaac
     1741 tgtaaaatgt tttggtaaaa aacaggttta tttagctggc atggtgctat gggcggtggg
     1801 tgatatactg aattatttct ggggaagtaa ctctttcact ttcgtcatgt tctcttgtgt
     1861 cgcctttttt ggcacggcct ttgtaaacag cctgaactgg gcactggtac cagataccgt
     1921 agattacggt gaatggaaaa ccggtattcg tgccgaaggt tctgtttata ccggttatac
     1981 cttctttcgt aaaatttctg ccgcacttgc tggcttcttg ccaggcatta tgctgacgca
     2041 aattggttat gttcccaaca tcgcgcaaag cgatgcgaca ttacagggtt tgcgtcagct
     2101 catttttatc tggccttgtg cactggcaat tattgctgca ttgacgatgg gattctttta
     2161 cacactcaat gaaaaacggt ttgcattgat tattgaggaa atcaaccaac gcaaaaataa
     2221 agagatggcg acagaaaaaa aaacggcttc cgtaacgtta taaatgtcaa tagccgctat
     2281 ttccatcctg gtgggtggcg gcctccctac gtttaaaaaa tggacttatt atgaaaaaga
     2341 ttaatgcaat aattttatta tcttctctga cctctgcctc ggtatttgct ggcgcatacg
     2401 tcgaaaacag ggaggcatat aatcttgcct ccgatcaggg cgaggttatg ctacgtgtgg
     2461 gttataactt cgatatgggc gcgggtatta tgttaaccaa tacctacacc tttcagcgag
     2521 aagatgaact aaaacatggc tataatgaaa ttgaaggttg gtatccttta ttcaaaccaa
     2581 ccgataaatt aaccatccag cccggtggct taattaatga taagagtatc agttcgggtg
     2641 gtgcagtgta tctggacg
//
LOCUS       DAZXUK010000376         2653 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZXUK010000376 DAZXUK010000000
VERSION     DAZXUK010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.378"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..327
                     /gene="hdeB"
                     /locus_tag="ACH3L8_004078"
     CDS             <1..327
                     /gene="hdeB"
                     /locus_tag="ACH3L8_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFT4086948.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            391..1037
                     /locus_tag="ACH3L8_004079"
                     /pseudo
     CDS             391..1037
                     /locus_tag="ACH3L8_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(1079..1609)
                     /locus_tag="ACH3L8_004080"
     CDS             complement(1079..1609)
                     /locus_tag="ACH3L8_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFT4086949.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(1765..2331)
                     /gene="slp"
                     /locus_tag="ACH3L8_004081"
     CDS             complement(1765..2331)
                     /gene="slp"
                     /locus_tag="ACH3L8_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFT4086950.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(2578..>2653)
                     /locus_tag="ACH3L8_004082"
     CDS             complement(2578..>2653)
                     /locus_tag="ACH3L8_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086951.1"
                     /translation="YGVEIEIPSGKRIRLSNHYQNIIP"
BASE COUNT          767 a          516 c          570 g          800 t
ORIGIN      
        1 atgaatattt catctctccg taaagcgttt atttttatgg gcgctgtagc ggctttgtca
       61 ctggtgaacg cacaatctgc gttggcagcc aatgaatctg ctaaagatat gacctgccag
      121 gaatttattg atctgaatcc aaaagcaatg accccggttg catggtggat gctgcatgaa
      181 gaaacagtat ataaaggtgg cgataccgtt actttaaatg aaaccgatct cactcaaatt
      241 cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa atttgtatac cttcaaaaat
      301 caagcatcta atgacttgcc gaattaatga ggtacaagta aaaaggagta gcaagttgag
      361 ccatcttgct gctccttttt gcatttttat atgacagcag aatttattat acgtcttata
      421 ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc aaatgcgcgg caaaggagca
      481 gggttacgca cacatgtatt aattggcatg ggaagcgccc tgtttatgat tgtttcgaaa
      541 tatggttttg ctgacgtgct gtctttagat cacgtcggac tcgaccccag ccgtattgct
      601 gctcaggtgg tgacgggcgt cgggtttatc ggtgcaggta acattctcgt tcgtaaccaa
      661 aatattgtag gtctgacgac ggcagcggat atctgggtga ccgccgcata ggtatggtta
      721 ttggcagcgg tatgtacgaa ctgggtattt atggttcagt gatgaccttg ttggtgctgg
      781 aagtcttcca tcaattaacc ttccgcctga tgaacaaaaa ttaccattta cagctgacat
      841 tagtgaacgg taatacggta tcaatgctcg actggtttaa gcagcaaaaa ataaaaactg
      901 atttagtttc gttgcaggaa aatgaagatc atgaagtggt cgccattgat atccagcttc
      961 atgccacaac gttgatagaa gacctgttgc gattattaaa aggtatggca ggggtgaaag
     1021 gcgtttcaat tagctaatct gtgtggcagc gtagccagac tcaccgtaag cctgaaattc
     1081 acaccagata atcaatatgc tgatggcgta ccagctcatt gatccgctta agattgagga
     1141 tcatcatgat attgtgttta tggcagtaga acgtttttag cgatattttc agtttctcca
     1201 gaatatcttc agttgtcatt tgattcgcaa tgcagctcag aacctgatgt tgcttgcgcg
     1261 aaagtgacac attcgcttca ggcacagagc agtaactgcc attgatgagt gcgacaaaat
     1321 caatgatagt gatagtccgc ggaacaaagg tcaccggtac tttccccaga cagcgtttaa
     1381 tattaaaggg ggagaaaaca atgacatgga caggttttaa tcgttcaatt tgagtgagaa
     1441 aattagaatg aaatacatta ttcattaatg tatcaataat gacgaaggca ttctgatgca
     1501 acattacgtc gatctcttct tcgttctgta tatgaacgac attaccttta ctcagaatgt
     1561 ttttcatcgc ggtgaagaac atcgtatccc tggtaattat aagaaacata accgactcct
     1621 ggtgcggaca ttaactttat aatcagtttg ccaaaggtaa ttaacaggac agggcttata
     1681 ataataatag ttagcttact gtatattaat tttatgtaat acatgatgca gatgctgaga
     1741 aagcatctgc atctttcggt ggtgttattt gaccagctca ggtgttacct gactcaccgc
     1801 attggtgtag taaggcgcac cccagcccgg ttccggccag aatgcaccat agccgtaatc
     1861 ccacaggtta taggtggtat taaccacttc tctcaaatgc cacacctgga tgccctgcat
     1921 attcacttcc aggaagttat acgggacttt attgataaag cctggttgtt caccctgaat
     1981 ggtaccgagg atggtaacaa agtgattacg atagttcact ggatcaagga agccgctttg
     2041 tctggcgagc agtcggccct gatagttggc ttcaatatca ggcttcgcat agctatccaa
     2101 cggtaataca gcgatttcta acaacgtatc cgttttgcca ttgataacgt tgataacctt
     2161 cccaccaaag cgcgcttgtt gaccaacata taaccccggc tggttatgaa cagcaacaaa
     2221 acttttttga atatcaggtt ggttattgcc tttgatattt tgcggaattg aactacatgc
     2281 ggcaagcaaa aatgaaaggc tgaggatgag tgcacctttt gtcatgttca tgttactatc
     2341 cttatcaaca aatattaaaa ccataataga tatttcttta tagttttcat ctgattctga
     2401 ggtgatatgc ctgatagtaa cgtaaacctg tctgatgttt atatcccttc tgtttagttc
     2461 aattccgtta ttttggggcg tggtatgagt atccatcgcc caataatata accctttgtt
     2521 ttaaaattac ataaaatcta tttttattca atgattataa agtgctaata atcaatatta
     2581 tggtattata ttctgataat gatttgataa gcgaattctt ttgccagatg gtatttctat
     2641 ttccacccca taa
//
LOCUS       DAZXUK010000377         2647 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZXUK010000377 DAZXUK010000000
VERSION     DAZXUK010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.379"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..530)
                     /locus_tag="ACH3L8_004083"
     CDS             complement(<1..530)
                     /locus_tag="ACH3L8_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4086952.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFV"
     gene            complement(576..2612)
                     /gene="yihQ"
                     /locus_tag="ACH3L8_004084"
     CDS             complement(576..2612)
                     /gene="yihQ"
                     /locus_tag="ACH3L8_004084"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFT4086953.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
BASE COUNT          627 a          691 c          700 g          629 t
ORIGIN      
        1 acgaatccca ccgtgcacag cagcaggccc agcgtagcgc cgccctgacg ccatgccgcc
       61 agtgaggcgc gttcgttggg gtttttggtg attgccggga ccattgcgcc gtaggagcag
      121 ttcatcatgc tgaaaaacag tccgtaaagc ataaacagaa tagtggccat caccgtttta
      181 ccggtgacat caaacggtgt gccgacaaag ttggcgatcg ccagtaaggt gaccgggaat
      241 gacgcataca gaataaaagg acggaactta ccttttggac cgatcttgcg tcgggaatcc
      301 aacataatgc cggtacccat atcggtaaac gcagtaaaga attttgaaat caaaaagata
      361 atgccgccat aggtgcctgg cagccccaga acgtcggtat aaaacttcaa aagataaagc
      421 gtaccgatat ccagcaggat gttagaggcc aggtcgccaa taccgtaaga gagtttctct
      481 ttaaagggca ggcgtaaagt tgctggatct tccgttgtga tgtgactcat cgatgttctc
      541 cctttgccca tgagtttccc cacgggcgga tgtgattaga tgctgtttaa cgacgcgaac
      601 agtgccgccc attcgctatc ggcgcgataa aagaccggcg gcttgccgat gggcgcatta
      661 acggtaactt ccccgccccg gaacgcttca cccgtccagg catgtaccca gttatcctcc
      721 ggcagataga gcgtccagtc gctgcgacct tcttcgtgta ccggagcgac cagaatgtcg
      781 cggcctaaca ggtactgata tttcagggaa taggtatgtg catcgtcttc gtaatgcagg
      841 aacagcgggc gcatgaccgg cagaccggac ttcgcattca gcgcgacggc ctctttcaga
      901 taaggtttca gggtggtgaa aacggtagtc atacgggcaa aatgggcgat agtttccgca
      961 tcgccgtcaa actgccagtt gtcgccagga cggttacctt cgtgggtgcg catcatcggc
     1021 gtgaaggcgc tgaaatcgca ccagcgcagc agcagctctt tgctgcgctt catctcaaac
     1081 agggtggtgt aaccgccaat gtcgctgtga tgcagaccat gtccggtcat tgccagcgac
     1141 agcgccgccg ggactacgga cgccaggcca tcgtcgagac tccagtccac gttctggtcg
     1201 cccgcccaca tcatggtgga gtatttctgg ctaccggtag aaccggcgcg cataaagaag
     1261 aggatctcgc cgagcttgcc cgtttcttca agggcttcgt agttacactt cgcccacagc
     1321 gcaggccagg cgttatgcat aatttcggca ctgacgccgt tatgcaggta cgtgtcggtg
     1381 ggcagatact cgccgaagtc agccatccag ccgccgcagc cgagttcaat catgttcttt
     1441 ttgatcactt ccttgaacca ggcgtaggct tctggattag tgagatcgac aacgccgccg
     1501 taaaactcgc caaactccac cagatagtca ccgccagagg catcttttgc tagatagccg
     1561 tgttgtgccg cttcttcgca gagatcttta tcgctggcaa catacgggtt gatataggcc
     1621 aggaactgca cgccctcctg ataccactgc ttaatgcgtg aatccagttg cgggtagttt
     1681 tcgctgttcc acttccagtt ccacatcacg cgcttgccaa aagaggttat acggatccca
     1741 gaccagtcct gcgcccagat gccgttaacc ttcacgcccg cgttacgcat ggtgtccagt
     1801 ttcttctggc acacttccgt cccgccctga atgccgagcg ttaccccgtc ataaatccag
     1861 tcgggcagtt ctggctggcg tcccagcagg gcagttaatt tttccagcag ggagatgtat
     1921 gtgtcagcac attcaaaacg tagcgtagct ttgtcttccc acagcgccag ttcatggtat
     1981 tccggggcgc taaagtcgaa gttcatgtag caactgttat caacatggca gtaatacttc
     2041 tgcgtgctga caaacgtagg ctgtgggaag aaagtccagt aatagtcgcc gcccgcattt
     2101 tctttgcagt cggcctgcca ggtgacatag gtttgtttgt tgcgaccaac gccttgttca
     2161 ctggtccata gcgggaacgg tttgccgcgc agatcgaagt aggaaaactg ttcgccgcag
     2221 ccgtagatat ggtcctctgg ttgagcggca aggcgcagcc agatacggtt gtggttaagg
     2281 ttgtcgtttt gtagttccag caataaacgg ccctgatcgt cggcagagat attcagcgtg
     2341 gcgctaatgt cagaaccacg gctgaaatga attaaccaac catccggtga ctggctgacg
     2401 atggcgtcgg taagcgcaat tttctcctgt agtttatctt taatgctgaa attaccgcgg
     2461 aacatatcga tatccgctat acctgagcca atccataaac aaggattatc tttgttatgg
     2521 gttaaaataa gacgttgttg aaaatgtagg gtaaaactgt cgttattctg atgaaattga
     2581 aaatctaata actgtggacg tggcgtatcc ataactaatc tccatagagt tgagaacgag
     2641 attgttg
//
LOCUS       DAZXUK010000378         2630 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZXUK010000378 DAZXUK010000000
VERSION     DAZXUK010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.380"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..591)
                     /locus_tag="ACH3L8_004085"
     CDS             complement(<1..591)
                     /locus_tag="ACH3L8_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFT4086954.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(658..1383)
                     /gene="fimC"
                     /locus_tag="ACH3L8_004086"
     CDS             complement(658..1383)
                     /gene="fimC"
                     /locus_tag="ACH3L8_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFT4086955.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVINNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1420..1959)
                     /locus_tag="ACH3L8_004087"
     CDS             complement(1420..1959)
                     /locus_tag="ACH3L8_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4086956.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2024..2572)
                     /gene="fimA"
                     /locus_tag="ACH3L8_004088"
     CDS             complement(2024..2572)
                     /gene="fimA"
                     /locus_tag="ACH3L8_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFT4086957.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT          679 a          675 c          580 g          696 t
ORIGIN      
        1 ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac cgggatccca
       61 taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag ggatcgtcag
      121 gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcctggacca ttgtggttaa
      181 tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg tattcagccc
      241 catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac tgtcgcccgt
      301 attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat cgacgcgata
      361 cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca cagcctgggg
      421 atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag gtgcctgtgc
      481 ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat gcttacgaat
      541 atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca ttattcctcc
      601 ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc ccctgcgtta
      661 ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg ttcggtaagt
      721 aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg gaggcaccaa
      781 tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt aatagggtgt
      841 cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt ctgcggcctg
      901 atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc tgataattgc
      961 gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg ctttaacgtt
     1021 catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat caagaatacg
     1081 taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga taaaacgacc
     1141 atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac tattttcatc
     1201 attatttatc acggcaagtt gcacttgttt ttgccctgcc ggataaatta cgcgagtcgc
     1261 acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga acatcaggat
     1321 acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt ttttattact
     1381 cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg ttaaagagaa
     1441 ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac gatatttggc
     1501 gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg gaggacgatt
     1561 aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg aggctatccc
     1621 tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac cgtgaaacgc
     1681 cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa caaaaggcac
     1741 cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc ccatattgac
     1801 cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga cgccctgaaa
     1861 ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag caaacattgg
     1921 cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta tcgcacaagg
     1981 gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga tactgaacct
     2041 tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca taataacgcg
     2101 cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta aatgtcgcac
     2161 cgtccagcgt cagagcattg cctgttctgt ctaggatctg cacgccaacg tttgttgcac
     2221 tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca attgccgtac
     2281 ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc agctgaatgt
     2341 taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta cgaacctgtc
     2401 ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg ttaacaactt
     2461 cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga gccgctgcgg
     2521 aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc atgctgcttt
     2581 cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact
//
LOCUS       DAZXUK010000379         2625 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZXUK010000379 DAZXUK010000000
VERSION     DAZXUK010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.381"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACH3L8_004089"
     CDS             complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACH3L8_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFT4086958.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIAEFAAGEIGEKEHLKASLQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
     gene            complement(1899..>2625)
                     /locus_tag="ACH3L8_004090"
     CDS             complement(1899..>2625)
                     /locus_tag="ACH3L8_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086959.1"
                     /translation="NIAEYIGENNQFTEKPATEYPPVIQDNTVTTKDYLYSNNIDTDL
                     LVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSCNLLS
                     LCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQSPADK
                     YDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIVTEKV
                     SSGRTAGGHHHRHTGYTWSVSVN"
BASE COUNT          679 a          690 c          624 g          632 t
ORIGIN      
        1 agcctgagtc acgtcttctc cggaggcaac ctccggttcg aagaggctgg ccatctggcg
       61 accttctgct tcccttgcag caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgtc tgatatttca gtgagagttt
      181 tactgccgga tacggtccgg gcagagtgac ataacaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctggcgctc catctcctta ccggtcgata ccccgtcacg
      301 cacggggtca gcaccgtagg aatactgcag actggctttc aggtgctctt tctcaccaat
      361 ttcaccggca gcaaactctg caatctgatg gctcggggag cggaaaaagg cacgggtatt
      421 cagcacatca aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagcctcc gaacttacgg gcttccggca ggatctccac
      541 caggtccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggaaaccac
      661 cggcttcagg gaggcatgag tgtcggcatt tgacgagata aacagccagc cgtttttctg
      721 atcttcccgg acaccccgca tccagtcacg tatggtgaag ggttcaccgt tatgctcaat
      781 cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga tggaaatcgc
      841 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg
      901 cagtttttcg attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
      961 gtcattacgc atcagatacg ccgcttccgc aaaaatggta cgtcctgatc cctgccagaa
     1021 cgggtcctct ttcgttccca tcgggatcag cgtgttcgcg acattatcaa aatccggctg
     1081 tgtcaggcac tccttccaca aatcccaggc ggcacagcgg gcatccagcg gattcaggat
     1141 cttatcgatg gagggatcat agtaactttt aacaaactca cacgaacggt cataaatcac
     1201 caccatgtcg ccacgtttac gggcatagtt ggcaagacgt cgaataacct ctgacttacc
     1261 cgtcgacact gttccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag
     1321 atcgccaatc cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt
     1381 cggattgtct gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     1441 acgccccaga atccaggaga caacaaagaa ggtaatcagg caaataacca gtgccacaac
     1501 tgtagccaga acgaatgcgg accatagctg ctctccgcac cagatcatat atttatcatg
     1561 cagtacctga gcagcgttca tccggaaggt tttgccgtaa tactggatct catagaccgg
     1621 ctgggatttt attaaatccc gcatgccttc cagtgacgta caccaccagt aaatacagcc
     1681 gttgataaac gtctgccagc ttatttttac ccataagacc agaccaatga ggatccagaa
     1741 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     1801 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg
     1861 ggaaaatctg gaacagggca gaaaaggatg atgtggggtt aattcacaga aacagaccag
     1921 gtgtaccctg tatgccggtg gtgatgcccg cctgcagtcc tgccggaaga gaccttctct
     1981 gtaacaatgt cagtcaggcg caatacttca gaccagtcac tcccatgcgg aaactggatc
     2041 tgaataaaaa ggcgtacccg ttcacggtga tccagaactg tgtccggttc ataaaaacgg
     2101 ccactgtcag gagaaacaca ggacgtttta ttgatgagat catacttatc ggcaggagac
     2161 tgcgatgaaa tcgcccaggt gatattctcc gcctggtggc catcaatacg actgctcccc
     2221 ggcgtccatg caatttcctg cgcactcgcc acgatgatct ccctggaaac ttcagccagc
     2281 tttctgggtg tacagaatac cggtgccagc acacacaggg acaacaaatt acagctgacc
     2341 gcctccggtt taagcaaatt gctcccggat ttttcaggaa aagatatttt tgtcagtatt
     2401 tcagttaatt ccttatgcct gagagaatta ataaagtaca tgataagcat catgagaaac
     2461 aatataacaa taagccctcc gataaccact aaaaggtcag tatcaatatt attgctgtac
     2521 agatagtctt tagtggtcac tgtattatcc tgtatcaccg gggggtattc agttgcaggc
     2581 ttctcagtaa attgattatt ctcaccaata tattcagcga tattg
//
LOCUS       DAZXUK010000380         2625 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZXUK010000380 DAZXUK010000000
VERSION     DAZXUK010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.382"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            623..1222
                     /locus_tag="ACH3L8_004091"
     CDS             623..1222
                     /locus_tag="ACH3L8_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFT4086960.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
     gene            1595..1978
                     /locus_tag="ACH3L8_004092"
     CDS             1595..1978
                     /locus_tag="ACH3L8_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFT4086961.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            1975..2400
                     /locus_tag="ACH3L8_004093"
     CDS             1975..2400
                     /locus_tag="ACH3L8_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFT4086962.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
BASE COUNT          651 a          637 c          661 g          676 t
ORIGIN      
        1 ggcttcaccc gtgttaatat cctggataac tgtgtttgaa ggatttctgt taataatctg
       61 ctcctgcaac tcacctgatg agtattagtg gtaatacata atcttactcg taaaagagta
      121 taccttatgt cattaaacag tcttccggcg tgttgttcaa tcctgtacct ctgttactta
      181 tctggtacgt tgatatgtgt aatgctacgt taattgcaat atctggttat ggctttatac
      241 cttcagcatt taaaatattt tattaccgga gaaacaggga agtggaaaga cttttgggat
      301 aatgtcaggc cattctgacg ttaatcacag taccaaataa cgagagggta atggaagtta
      361 aaacctgcgt aagttcctct gttgttcact ggaaaaagtg attgcccaca cgcgttataa
      421 aggcctttcc gctgcagcct ggttaacaga gcttcaggcg ttatatgcgc tgtatggctg
      481 ttacgtatgc tgttctttgc ctggttctga tacccgtgga agtcgttacc ggaggaaaca
      541 cgttcaccct actacctgag aacatcttct tcaaaaatta cggcaacgct tcttcctgtg
      601 ttacagaaca cgaggaagaa acatgcaaac ttataacacc aatcccgctt acgaaatggc
      661 tccccagctc ctggagcact ttaaccagca tctggattca ttattcgggg tctattcgaa
      721 actgttaccg ttcagaatgg attttgctta ccgaaaaaac accctcagct acagatgtgc
      781 ctgcaggtac gccatgtgcg cggagatgct gcaactgatt aacgaggtgg gagagaaact
      841 ggtcgggtat gcctgggtga tggaatatac agaacgtaaa gggcttcaca ttcattttgt
      901 gggctatctg aacggccaga gccacaggag ttcgtatctg gtatccagat tgatgggaga
      961 tatctggagg cgggttaccg agggtaatgg ttactaccac tggtgccggt ttaataaaaa
     1021 ttatccggta aacattaacc acgtcattca ctattcagat cacaaagcgg tgaatgattt
     1081 acgctatgcc atcagctacc tggccaaacg ggaacagaaa gaatgtggca tcatcctgaa
     1141 atgcagcggg cttcctgaaa aaagtaaccg gggtcgacca cgactggaca gccccctgcc
     1201 ggggatatgt gctctggtgt aaacccctga acgacaagta cgggctggcg ctaatctttg
     1261 ctgtcggttc cggcggccac ttcagcggca ctgcgcattt tttacggcgt ggtcattccg
     1321 tatgaaccat acggagattt aaccatgaca cattctttac cattccttcc ggcaaccgat
     1381 acagcacatc ccccaatctc catctctgct tactgactgt tttttacgtc agtgggccat
     1441 tgtgtgacaa ccacagtgct gtaacagcaa ccagcctgct ttacccaaac tccagtccag
     1501 attttaactg aaccgccatt cacaccctga ccacgctgcc cgtaccggga cggctgttgc
     1561 ctgcgtgtcg tctggcggct gaggaacata tactatgtac gcaaaatctt ttctcgcact
     1621 tgatggcaac ggacgtctga ccggtgcccg taccgcacag actgcacctt acgatagcta
     1681 cacctgccac ctttgcggca gtgcactcag ataccacccg caatacgaca ctgaacggcc
     1741 ctggtttgaa cacgctgacg aagggctgac ggagcacggt catgagtgcc cttatgtcag
     1801 gccggaacgc agagaaatac ggttgattaa acgtctgcag caattcgtcc cggatacctt
     1861 acccgtagta cgtaaagcca gctggcactg caggcaatgt caccacgatt attatgggga
     1921 gcagtactgc acacactgcc agaccgggcg cttcagtgag gaggggggag cagaatgaac
     1981 ggtataaaaa cggggctggg gatcacgccg ggtgagcaca ttatcagtgc cgactctgcg
     2041 ctcagcagaa atatccggca gtgtttctgc ctttcctgcc atggccggtt gattctgcag
     2101 acagatgctc agggggcgtg gtttgagcat gatttacatg ccctgagtga gcaacagaag
     2161 gcggactgtg tggtgctgaa tccggagaag tcccaccctt acattgagga tatggcgatg
     2221 tttttatcgc cattaccggt cgtgctggag tggcattgtg tgatgtgtga gcagtttttt
     2281 catgggaaga agttctgtga ggcctgtggt acggggattt attgcagagc ggtatgtacg
     2341 aagtctgttt acagctacca gccagacttg tttgaggact gcggaggtgc gagtacttaa
     2401 tccgaaatgc ccgacctgct cagggcgttc agaggtattg ccaacctgta ttttatctgg
     2461 tgtttcgcac ccaccccgtt cgtgacacaa aggcgcgaca ccggatatcc gcagtcctgc
     2521 ttacctgaat aaaacccggg gtattgattg gtagtgtggt gcggaatcgt accgtgatct
     2581 cgcattcgcc gaattcgcac tgtggtgtca atcaaccata cgcga
//
LOCUS       DAZXUK010000381         2622 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZXUK010000381 DAZXUK010000000
VERSION     DAZXUK010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.383"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            106..1071
                     /gene="dusB"
                     /locus_tag="ACH3L8_004094"
     CDS             106..1071
                     /gene="dusB"
                     /locus_tag="ACH3L8_004094"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFT4086963.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1097..1393
                     /gene="fis"
                     /locus_tag="ACH3L8_004095"
     CDS             1097..1393
                     /gene="fis"
                     /locus_tag="ACH3L8_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFT4086964.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1479..2363
                     /gene="yhdJ"
                     /locus_tag="ACH3L8_004096"
     CDS             1479..2363
                     /gene="yhdJ"
                     /locus_tag="ACH3L8_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFT4086965.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2447..>2622
                     /locus_tag="ACH3L8_004097"
     CDS             2447..>2622
                     /locus_tag="ACH3L8_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFT4086966.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
BASE COUNT          724 a          615 c          646 g          637 t
ORIGIN      
        1 ctttgatcca tctcagagga ttggtcaaag tttggccttt catctcgtgc aaaaaatgcg
       61 taatatacgc cgccttgcag tcacagtatg gtcatttctt aactcatgcg catcggacaa
      121 tatcagctca gaaatcgcct gatcgcagcg cccatggctg gcattacaga cagacctttt
      181 cggacgttgt gctacgagat gggagccgga ttgacagtat ccgagatgat gtcttctaac
      241 ccacaggttt gggaaagcga caaatctcgt ttacggatgg tgcacattga tgaacccggt
      301 attcgcaccg tgcaaattgc tggtagcgct ccgaaagaaa tggcagatgc agcacgtatt
      361 aacgtggaaa gcggtgccca gattattgat atcaatatgg gttgcccggc taaaaaagtg
      421 aatcgcaagc tcgcaggttc agccctcttg cagtacccgg atgtcgttaa atcgatcctt
      481 accgaggtcg tcaatgcagt ggacgttcct gttaccctga agattcgcac cggctgggca
      541 ccggaacacc gtaactgcga agagattgcc caactggctg aagactgtgg cattcaggct
      601 ctgaccattc atggccgtac acgcgcctgt ttgttcaatg gagaagctga gtacgacagt
      661 attcgggcag ttaagcagaa agtttccatt ccggttatcg cgaatggcga cattactgac
      721 ccgcttaaag ccagagctgt gctcgactat acaggggcgg atgccctgat gataggccgc
      781 gcagctcagg gaagaccctg gatctttcgg gaaatccagc attatctgga cactggggag
      841 ttgctgcccc cactgccttt ggcagaggtt aagcgcttgc tttgcgcgca cgttcgggaa
      901 ctgcatgact tttatggtcc ggcaaaaggg taccgaattg cacgtaaaca cgtttcctgg
      961 tatctccagg aacacgctcc aaatgaccag tttcggcgca cattcaacgc cattgaggat
     1021 gccagcgaac agctggaggc gttggaggca tacttcgaaa attttgcgta aacagaaata
     1081 aagagctgac agaactatgt tcgaacaacg cgtaaattct gacgtactga ccgtttctac
     1141 cgttaactct caggatcagg taacccaaaa acccctgcgt gactcggtta aacaggcact
     1201 gaagaactat tttgctcaac tgaatggtca ggatgtgaat gacctctatg agctggtact
     1261 ggctgaagta gaacagcccc tgttggacat ggtgatgcaa tacacccgtg gtaaccagac
     1321 ccgtgctgcg ctgatgatgg gcatcaaccg tggtacgctg cgtaaaaaat tgaaaaaata
     1381 cggcatgaac taattcaggt tagctaaatg cttgattaaa aaggcgctac tcggcatggg
     1441 gaagcgcctt ttttataggt gtcacaaagg gagtgaccat gagaacagga tgtgaaccga
     1501 cccggtttgg taatgaagct aagaccatta ttcacggtga tgcctttgcc gaacttaaaa
     1561 agctacctac tgaaagcgtc gatctgctct ttgccgaccc accgtataac atcggtaaaa
     1621 attttgatgg tctgatcgaa gcctggaaag aagatctgtt tatcgactgg ctgtttgaag
     1681 tgattgcaga gtgccaccgc gttctgaaaa agcagggcag catgtacatt atgaacagta
     1741 cggaaaacat gccctttatc gatctccagt gccgcaagct ttttaccatc aaaagtcgca
     1801 tcgtctggtc atatgacagt tctggagtac aggcgaaaaa acactacggc tccatgtacg
     1861 aacccatcct gatgatggtg aaagacgcaa agaactacac attcaacggt gatgctattc
     1921 tggtagaagc caaaaccgga tcgcagcgcg cgttgatcga ttatcgcaaa aatcctccac
     1981 agccatacaa tcatcaaaaa gtaccgggta acgtctggga ttttccgcgc gtgcgttatt
     2041 tgatggatga atatgagaac cacccgacgc aaaaaccgaa agccttactg aaacgcatta
     2101 ttctcgcctc ttccaaccca ggcgatatcg ttctcgaccc gtttgccggt agctttacta
     2161 ccggtgccgt agccatcgcc agcggacgaa aattcattgg tattgagatc aacagcgagt
     2221 acatcaaaat ggggcttcga cggctggatg tcgcgtcgca ttactccgcg gaagaactgg
     2281 cgaaagtgaa aaaaagaaag accggcaacc tgtcaaaacg aagccggtta agcgaagttg
     2341 accccgatct cattgcaaag taaaggaagt gtaagcctag ttttcagatt attcatttcg
     2401 tgtatatttc ctgccagact tggttaaaca tgcacaggct caggtaatga ttcgcaagta
     2461 ttggtggctc gtcgttttcg ctgtcttcgt ttttctgttt gatactttac tgatgcagtg
     2521 gattgaactg ctgacaacag aaacagacaa atgccgtaat atgaactcag ttaatccact
     2581 aaaactggtt aactgtgacg aactgaattt tcaggacaga at
//
LOCUS       DAZXUK010000382         2617 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZXUK010000382 DAZXUK010000000
VERSION     DAZXUK010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.384"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..125)
                     /locus_tag="ACH3L8_004098"
     CDS             complement(<1..125)
                     /locus_tag="ACH3L8_004098"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="with CysN catalyzes the formation of
                     adenylylsulfate from sulfate and ATP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase small subunit"
                     /protein_id="HFT4086967.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSV"
     gene            377..1414
                     /gene="iap"
                     /locus_tag="ACH3L8_004099"
     CDS             377..1414
                     /gene="iap"
                     /locus_tag="ACH3L8_004099"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFT4086968.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(1560..2617)
                     /locus_tag="ACH3L8_004100"
                     /pseudo
     CDS             complement(1560..2617)
                     /locus_tag="ACH3L8_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
BASE COUNT          719 a          604 c          643 g          651 t
ORIGIN      
        1 acgctggaat ctttaccgat agagtagagc atcaccggat ttgagaattc tgccgccacc
       61 tcgcgaataa tgtggatgct ttccgcctcc agttgccgca ggtgagtaag tcgtttttga
      121 gccataaccg ttcctttgca ataccgctat tttcttgcca tcagatgttt cgactatagg
      181 gagcgtaaga gaacgaatga aattaccaat tagaatgagt agttccttaa cggaataacg
      241 atttggcaaa gctaatatca aaaagtgctt aaggcaccgg atttcgggcg tttaggaaga
      301 tttgaaattg ttttagcgca gcggcagttt catactatgg cggtaaaaaa atttgcatgg
      361 tatttaagga ctcactatgt tttccgcatt gcgccaccgt accgctgccc tggcgctcgg
      421 cgtatgcttt attctccccg tacacgcctc gtcacctaaa cctggcgatt ttgctaatac
      481 ccaggcacga catattgcta ctttctttcc gggacgcatg accggaactc cagcagaaat
      541 gttatctgcc gattatattc gccaacagtt tcagcaaatg ggttatcgca gtgatattcg
      601 gacatttaat agtcggtata tttataccgc ccgcgataat cgcaagaact ggcataacgt
      661 gacaggaagt acggtgattg ccgctcatga aggcaaagcg ccgcagcaga tcatcattat
      721 ggcgcatctg gatacttacg ccccgctgag cgatgctgac gcagatgcca atctcggcgg
      781 actgacgtta caaggaatag atgataacgc cgcaggttta ggtgtcatgc tggaactggc
      841 agaacgcctg aaaaatacgc ctaccgagta tggtattcga tttgtggcga ccagcggcga
      901 agaggaagga aaattaggcg ctgagaattt actcaagcgg atgagtgaca ccgaaaagaa
      961 aaatacgctg ctggtgatta atctcgataa cttaattgtt ggcgataaat tgtatttcaa
     1021 cagcggtgta aaaacccctg aagcagtaag gaaattaacg cgcgacaggg cgctggcaat
     1081 tgcacgcagt cacggaatag ccgcaacgac caatccgggt ttgaataaaa attatccgaa
     1141 aggcactggg ggttgtaatg acgcagaaat attcgacaaa gcgggcattg ctgtactttc
     1201 ggtggaagcg actaactgga atcttggaaa taaggatggt tatcagcaac gcgcaaaaac
     1261 acctgccttc ccggcgggaa atagctggca tgacgtaaga ctggataatc aacaacatat
     1321 tgataaggca cttcctggaa gaatagaacg tcgctgccgt gacgttatgc ggataatgct
     1381 accgttggtg aaggagctgg cgaaggcgtc ttgatgggtt tgaaaatggg agctgggagt
     1441 tctaccgcag gggcggggaa ttctaagtga tacccatcat cgcatccagt gcgtccggtt
     1501 tacttccggg aaaaccgcac cgtattgcgc cggtttatct ccgctggagc ggggaacacc
     1561 tctggcgaat agaaagcaag ccaggttttg accagtcgat cgtacatggc attggccgtt
     1621 gccattgttg gcaggccaaa aaacagcccc tgagcgttac ccgcagccat caacctgtgc
     1681 gccaggataa gcgccgcttc tgttttacct gcgccggtca catcttccag aataaacaac
     1741 tgtggtcctg gctgattgat atccagatcc agcacctttt gctgtaacgg tgtcgtattc
     1801 tcaataaaag gaaacagcgt attaattccg gtgaaaggcg cggtttctgc ttgtggcgga
     1861 aagacggtta atgcgttttg agcctgaact aaagcctttt gccagtaatc ttcaatatcc
     1921 attgcctgtg cgacgcgtgg aaaaaatcgc gttgacgacc ctgtccagtc tgcaagtacg
     1981 acggttgcag agatatacca ggaaagttgt tttataagtt caacgccctc gtcatcatcc
     2041 cagaatgtgg ggatctctat tcgcggaaac agtggcttga tttcaaggag aaaatctcgt
     2101 gctgcagctt tgtcttcagg cagaaaatta tccaattcat ccatacgatc agggggccga
     2161 ccatgatgcc ctgtcgtcac gggcatccac atttctatca ctcgcctaag tttgcgcgga
     2221 gagagcgaag atgaaggaag taactcctga cattcactta aataatgatt ccacaaccag
     2281 tagcccagcg ttgaatgaga gattttttcg taattttttc tggcaccttc ctgaatcttg
     2341 aattcagggg gcaggtaaag ttgttgaaaa gagcgggcaa attttccaat atcgtgccag
     2401 cacagcaacc aggcgaaaaa tcgagctgcc tgttccttgt cagaaatccc taattgacga
     2461 aaataatcag ccagcccgaa gcaatttctt ttaaccatta aatagcccat tgcggccaca
     2521 tccagcaaat gccagcaaag aaggtgatag tcgtcgatac cctctttctc gccacgtcgg
     2581 ttttttcccc agaaatcaaa gaaagtcaca atatttt
//
LOCUS       DAZXUK010000383         2607 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZXUK010000383 DAZXUK010000000
VERSION     DAZXUK010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.385"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..338)
                     /locus_tag="ACH3L8_004101"
     CDS             complement(<1..338)
                     /locus_tag="ACH3L8_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFT4086969.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="ACH3L8_004102"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="ACH3L8_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(992..1338)
                     /locus_tag="ACH3L8_004103"
                     /pseudo
     CDS             complement(992..1338)
                     /locus_tag="ACH3L8_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(1445..2008)
                     /locus_tag="ACH3L8_004104"
     CDS             complement(1445..2008)
                     /locus_tag="ACH3L8_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFT4086970.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
BASE COUNT          785 a          581 c          523 g          718 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgta
//
LOCUS       DAZXUK010000384         2589 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZXUK010000384 DAZXUK010000000
VERSION     DAZXUK010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.386"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..2450
                     /gene="lpfC-O113"
                     /locus_tag="ACH3L8_004105"
     CDS             <1..2450
                     /gene="lpfC-O113"
                     /locus_tag="ACH3L8_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFT4086971.1"
                     /translation="VSVFNPALLEINHQSGVDIRQFNRANLMPPGVYSVDIFINGKMF
                     ERQDVTFVQDNPDADLHACFIAIKKTLSSFGIKVDALKSFNDVDETVCLDPAPRIEGS
                     SWQFDSDKLQLNISIHQIYMDAMAYDYISPTRWDEGINALTINYDFSGSHTLRSDYGS
                     QETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGRAEYNSISTWIQRDIAALRSQIMIGD
                     TWTASDIFDSTQIRGARLYTDNDMLPASQNGFAPVVRGIAKSNATVIIRQNGYVIYQS
                     AVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQRFTQPYASLAILKREGLTDVDVSVG
                     ELRDEDGFTPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDV
                     THARANFSHDDTETGQSYRFLYSKLFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSP
                     EDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRL
                     SWNVSWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLSGWWGNSYATYSVSQNDNSG
                     SSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQ
                     RLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAARLEIDNMRGAATDWRGYTVKTQ
                     LNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNAN
                     GKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSF
                     STPESESGLIEQGVTCH"
     gene            2461..>2589
                     /locus_tag="ACH3L8_004106"
     CDS             2461..>2589
                     /locus_tag="ACH3L8_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4086972.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          691 a          561 c          641 g          696 t
ORIGIN      
        1 aggtcagtgt attcaatccg gcgcttctgg aaatcaacca tcaatccgga gtcgatattc
       61 gccagtttaa tcgggcaaac ctgatgcccc caggtgttta tagcgttgat atttttatca
      121 acggtaaaat gtttgaacgt caggatgtga catttgttca ggataatcca gatgctgatc
      181 tgcacgcttg ctttattgcc attaaaaaaa cactgtcctc ctttggcata aaagttgatg
      241 cgctcaaatc gttcaatgat gtggatgaga cggtttgcct cgatcctgct ccacgtattg
      301 aaggctcatc ctggcagttt gacagtgata aattgcagct gaatatatcc attcaccaaa
      361 tctacatgga cgcgatggct tatgattaca tcagccccac gcgttgggat gaggggatta
      421 atgcgctcac catcaactac gatttttctg gttcacatac actacgttca gattatggtt
      481 cacaagagac agataccagt tatctcaatc tgcgcaatgg actgaatatt ggaccgtggc
      541 ggctacgtaa ttacagtact ttaaacacca gcgatggccg tgcggaatac aactccatta
      601 gtacctggat acagcgcgat attgccgcgt taagaagcca gattatgatt ggtgatacgt
      661 ggacggcgag cgatattttc gacagtacgc aaattcgcgg cgcgcgtttg tatactgata
      721 acgatatgct acccgccagc cagaatggct ttgctcctgt ggttcgtggg attgcaaagt
      781 ccaacgccac cgtcatcatt cggcagaatg gctacgtgat ttatcagtca gccgttccac
      841 aaggtgcttt tgagatcacc gatctcaaca ccgcaagtac aggtggcgat ttggacgtaa
      901 ccatcaaaga agaagacggt agcgaacaac gattcaccca accttatgct tcattggcga
      961 ttcttaaacg tgaaggtctg acagatgttg atgtcagcgt gggtgaattg cgcgatgaag
     1021 acggatttac accggacgtc cttcaggcgc aaatacttca tggtttttcc cacgggatca
     1081 ctttatatgg aggtatgcag gctgctgaaa attatggttc tgcagctctg ggtgtcggta
     1141 aagatcttgg cgctttgggc gcaatttctt tcgatgtgac acatgctcgt gcgaatttta
     1201 gccatgatga tacagaaacg ggtcagtcat atcgctttct ctattcaaaa ctatttgacg
     1261 acacagacac tagcttgcgc ctggttggct atcgttactc caccgagggc tactataccc
     1321 tcaatgagtg ggcatcgcgg cgcaacagcc ctgaagactt ttgggaaaca ggtaaccgac
     1381 gtagtcgcgt ggagggaacg ctaacgcagt cgttggggag agattatggc aatttatacc
     1441 tgacattaag ccggcaacaa tactggcata ccgatgatgt cgaacgatta atgcaatttg
     1501 gctacagcag tagctggaag cgtctctcgt ggaacgtctc ctggagttat tccaatactg
     1561 ccagacaggg gacggggaac aaccatgcca gtgataacac cagtgagcag atctacatgc
     1621 tctctttatc tgttccttta tcgggctggt ggggtaatag ttacgccacc tattctgttt
     1681 cgcaaaacga taattccggt agctcacatc aactcggact cagcggtacg gcgctggaaa
     1741 gaaataacct ttcatggaat ttaatgcagt cctataacag tcatgatgat gaggttggcg
     1801 gtaatatgtc cctgacctat gatggctctt atggcacggt gaacggcagc tataactaca
     1861 gccaaaattc ccagaggctg aattatggta tcagaggggg aattctggca cacagcgaag
     1921 gggtaacgtt aagtcaggag ttaggtgaaa ctattgctct tgttaaagca cctggggccg
     1981 ccaggttaga aatagataat atgcgcggtg ctgcgacgga ctggcgcggc tatacggtca
     2041 agacacagct aaacccttat gatgaaaatc gggtagcaat cagcgataac tatttctcga
     2101 agtcgaatat agaacttgat aataccgtcg ttacgatggt tcccacgcgt ggtgcagtgg
     2161 ttaaagcgga gtttgtgact catgtgggtt atcgcgttct cttcagagtg ttaaatgcaa
     2221 atggtaaacc ggtacctttt ggagccattg ctgcgataca agatgcaagt ttggcagatt
     2281 caggaattgt cggtgaccgt ggcgaacttt atctttctgg tctaccagaa aaaggacagg
     2341 ttacgttatc ctggggagaa aacgcctcaa caaaatgcat cttcaattat tcattttcga
     2401 caccagaaag tgagagcgga ttaattgaac agggtgtgac atgtcattaa aaggattttt
     2461 atgaacaagt atatcaaaca gtggtgcttt gctgtgttta tgctctcgtt aagtagtgta
     2521 gcacttgcag ctcctaaagg tatctgtacc cctgataatg gcgtatttca cagtacgctt
     2581 gatttctcg
//
LOCUS       DAZXUK010000385         2539 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZXUK010000385 DAZXUK010000000
VERSION     DAZXUK010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.388"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            173..1714
                     /gene="istA"
                     /locus_tag="ACH3L8_004107"
     CDS             173..1714
                     /gene="istA"
                     /locus_tag="ACH3L8_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HFT4086973.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            1729..2475
                     /gene="istB"
                     /locus_tag="ACH3L8_004108"
     CDS             1729..2475
                     /gene="istB"
                     /locus_tag="ACH3L8_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HFT4086974.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
BASE COUNT          726 a          598 c          607 g          608 t
ORIGIN      
        1 atcactcaat aacttccatc gagatcgggt aataacattt gaacagatcg ctgaataaca
       61 tcgatggaga tcacttttga ctcattttgt tattcagtga tctccatcaa tgttattgga
      121 acttcacagg tgtgttgatc tgtatctttt gccattccgg taaaggatac ctatgccaac
      181 agttccaatt tctatgagaa aacttaaaga aattcttagg cttaaatacg gtgttggact
      241 cagccatcga caaattggtc gtagtcttgc aatctcccct tccgttgtat ccagatatgc
      301 taatcgggcg gctcaacttg gcataaagca gtggccctta cctacaggat gggatgatac
      361 aaaactaaaa catgcgttcc ttcagaccca ggttaagatg aagaagcact ctctgcctga
      421 ctgggctaca gtacaccggg aactgcgtaa taaatgcgtg acgctgcagc tactctggga
      481 agaatactgt gagcgtaatc caggcggttt ttacagctat aaccattact gccggatgta
      541 ccgtgaatgg ctcaaaacca cttcaccatc aatgcgtcag gtacataaag ctggcgaaaa
      601 acttttcgtt gattactgtg gacctaccgt tggcgttacc gaccctgaga ccggagaaat
      661 aagaactgct caggtcatcg tagctgttct cggggcatca agttacacat gggcagaggc
      721 cacctggtct cagcagcttg aagactgggt gatgagtcat gttcgctgct tccagtggtt
      781 gggtggcgtt cctgaacttg ttgttccgga caatctgaaa agcgccacat ccagggcatg
      841 taagtatgat cctgacgtta accctaccta ccagcagatg cttgagcatt ataatgtcgc
      901 agttttgcct gcgcggccac gtaaaccgaa agataaagcc aaagctgaag ttggcgttca
      961 ggttgttgaa cgctggatca tggcccgaat caggcatgag atcttctaca gccttgcatc
     1021 gcttaatcag cgcattcggg agttgctgga aagactgaat aacaaaataa tgcagaagtt
     1081 gggttattca cgtgcagaac tcttcatcca gcttgataaa cccgcactga agcctcttcc
     1141 tgaagccagt tacagttaca ccctggtgaa gaaagtcaga gttcatgccg attaccacgt
     1201 ggaaatcgac aaacattact actcggttcc atgttcgctg ttaggccagc aactggaagc
     1261 atggatctcc ggagaactgg taagactctt caatcagggg caggaggttg ctgtgcaccc
     1321 gcgcaagcgt acttatggct acagtacccg caacgagcac atgcctgaag ctcatcgaca
     1381 gcatgccacc tggacgccag agcgtcttct ggaatgggcg gggcacatag gcagtgaaac
     1441 tcatagttat gtgcttcata tactgaactc tcgtccacat ccggaacaaa gctatcgctt
     1501 ctgccttgga ctcctgaacc ttcataaaaa atacagtaaa gccagactta atgcagcatg
     1561 tgcaagagct ctgaaaacaa aggtatggcg tctgtcaggt attaaatcga tcctggaaaa
     1621 aggtctggat aaacaacctg ttcaggatcc aaaaccagat ctgttatcca cgatggaaca
     1681 cgaaaacgta cgcggcagtg agtattacca ctgatacggg atccaatgat gaatcatctt
     1741 tacgaacaac tgaccgcact taaactcacc ggcttccgtg atgcgcttaa aaagcaactt
     1801 gctcagccgg gcacatacca ggagctgggc ttcgaagaac gcctgtcatt actgacagca
     1861 gaagaactaa cctgccgtga aaacaggaag gcagagcgtc tgatcaaaca tgcacggttc
     1921 agacttaatg ctgagttatc aaagctggat tatcgtaaca atagagggct ggacagggcc
     1981 ctcatccgtt cactcagtca gggaaactgg ttaaccctga aacaaaatat tttactgacc
     2041 ggggccaccg gcagcggtaa aacgttcctg gcatgtgcac ttggtcataa tgcctgccga
     2101 cagggataca aggtctacta ttatcgcctt aaagcgctga tggaacagtg ctatcagggg
     2161 catgctgatg gaagatacag caaacttttg accaggctga ataatagcga tctgctgctt
     2221 ctggatgact gggggctgga acctctctca tcagaacagc gtagcgacct gctggaaata
     2281 gtggatctga tgtaccaacg aggctcaatc atcgtagtga gccagttgcc ggtggaaaac
     2341 tggtacaaaa tgatcggaga ctccacacat gcggatgcca tcctagatcg actggttcat
     2401 ggcagtatca agatcgaact taaaggagaa tcaatgcgga aaatacaatc tccgttgacc
     2461 gaaggagatc agtgaaggta atttaaaaac ggttctgtga aagtgacacg aaccgatctc
     2521 catcgatgtt actcaccga
//
LOCUS       DAZXUK010000386         2519 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZXUK010000386 DAZXUK010000000
VERSION     DAZXUK010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.389"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="ACH3L8_004109"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="ACH3L8_004109"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFT4086975.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="ACH3L8_004110"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="ACH3L8_004110"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFT4086976.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1133..1891
                     /gene="tam"
                     /locus_tag="ACH3L8_004111"
     CDS             1133..1891
                     /gene="tam"
                     /locus_tag="ACH3L8_004111"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFT4086977.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1895..>2519)
                     /locus_tag="ACH3L8_004112"
     CDS             complement(1895..>2519)
                     /locus_tag="ACH3L8_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFT4086978.1"
                     /translation="REVKTTLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYL
                     KTGDLQRVLASNSPANRILLIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCE
                     RIAYTPIPFAYTLILHRTVYLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEE
                     LEDPFGTENNDLPLDAICNAIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          640 a          587 c          679 g          613 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga
     1141 ctggaacccc tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
     1201 gcttgccaga gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg
     1261 taacagcacc gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc
     1321 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
     1381 agacatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     1441 gcaatggctg cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
     1501 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
     1561 cgaagttgct tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
     1621 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta
     1681 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     1741 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca
     1801 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
     1861 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg
     1921 gaagaatttt tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag
     1981 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccagtt
     2041 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2101 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg
     2161 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt gtaggcaatg cgctcacagc
     2221 ctgccagaac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     2281 aaagctgtcc attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt
     2341 tagctggaga gttcgaagcc agtacacgct gaagatctcc agtcttgaga taatgagcca
     2401 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     2461 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctctcgc
//
LOCUS       DAZXUK010000387         2513 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZXUK010000387 DAZXUK010000000
VERSION     DAZXUK010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.390"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..190
                     /locus_tag="ACH3L8_004113"
     CDS             <1..190
                     /locus_tag="ACH3L8_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4086979.1"
                     /translation="MVRKFGKQLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHT
                     KNELTGGKRRYRIKNGCK"
     gene            437..586
                     /locus_tag="ACH3L8_004114"
     CDS             437..586
                     /locus_tag="ACH3L8_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFT4086980.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            871..1119
                     /locus_tag="ACH3L8_004115"
     CDS             871..1119
                     /locus_tag="ACH3L8_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFT4086981.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            1364..1438
                     /gene="tap"
                     /locus_tag="ACH3L8_004116"
     CDS             1364..1438
                     /gene="tap"
                     /locus_tag="ACH3L8_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFT4086982.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1431..2288
                     /gene="repA"
                     /locus_tag="ACH3L8_004117"
     CDS             1431..2288
                     /gene="repA"
                     /locus_tag="ACH3L8_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFT4086983.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          654 a          603 c          664 g          592 t
ORIGIN      
        1 gatggtcaga aaatttggta aacaacttat tgtcgctgta agtgaaaagg atgccatcaa
       61 attaaaagac aactttgata ttattgggat gttgtcatat ggtaaagaaa attttattgc
      121 catgagtcat acgaaaaacg agttaactgg aggaaaacgt cggtaccgga ttaaaaatgg
      181 atgtaaataa aaagcatact tattggctgg agtgacgcct tcgtgcttct gtagtaaaaa
      241 gcgggcaatg caggtaatag tcagcaacag ggaatgtggt attatcgcgg cgggtgtctg
      301 agcctttctg gttcaggcaa gacgcaggta ccagaaatgc aaagacccca cttgttaatc
      361 cattaactcg tgaggtctgc atgaagtaca ttgcactact gattgtagcc tcttccttgc
      421 agagaggtca aagtttatga cgaagtatgc ccttatcggg ttgctcgccg tgtgcgccac
      481 ggtgttgtgt ttttcactga tattcaggga acggttatgt gagctgaata ttcacagggg
      541 aaatacagtg gtgcaggtaa ctctggccta cgaagcacgg aagtaaactg ccgggcgggg
      601 acggaagtcc ccgctttccg gaagtgtgag gtatttcagg ggcagacacc cgacatgcca
      661 gaaacagccg gtcccgcccg gggccggctc ccgggttaag gcatttcctg ttttttcagc
      721 catttcgcaa caaaaatcac attaaacaac agtaatcccc gttgatttgt gcgccaacac
      781 agatcttcgt cacaattctc aagtcgctga tttcaaaaaa ctgtagtatc ctctgcgaaa
      841 cgatccctgt ttgagtattg aggaggcgag atgtcgcaga cagaaaatgc agtgacttcc
      901 tcatcaggta caaagcgagc atacagaaaa ggtaatcctc tgacgcttgc agaacgtcag
      961 caggcatccc tggcaagaaa acgagctaca cataaagaac tcagagtgtt cattcctgct
     1021 gcgttaaaag cgcaattgca ggtgatgtgt gaagctgagg gcgttaccca ggctgagatg
     1081 attgccgaac tgataaagca gaagagtgct tttagctaac taaaatgtag cgacatcaca
     1141 ttcttgcctg tttctggtgt gcgtgataga ttgctgatcg tttaaggaat tttgtggctg
     1201 gccacgccgt aaggtggcag ggaactggtt ctgatgtgga tttacaggag ccagaaaagt
     1261 gaaaaccccg ataatcttcg acaagtttgg cgactgagaa gattaccggg gccatctaaa
     1321 aaccgtatag ccaacaattc agctatgcgg ggagtatagt tatatgcccg gaaaagttca
     1381 agacttcttt ctgtgctcac tccttctgcg cattgtaagt gcaggatggt gtgactgatc
     1441 ttcaacaaac gtattaccgc caggtaaaga acccgaatcc ggtgtttaca ccccgtaaag
     1501 gtgccggaac gctgaagttc tgcgaaaaac tgatggaaaa ggcggtgggc ttcacttccc
     1561 gttttgattt cgccattcat gtggcgcatg cccgttcgcg tggtctgcgt cgacgcatgc
     1621 caccggtgct gcgtcgacgg gctattgatg cgctcctgca ggggctgtgt ttccactatg
     1681 acccgctggc caaccgcgtc cagtgctcca tcaccacgct ggccattgag tgcggactgg
     1741 cgacggagtc tgctgccgga aaactctcca tcacccgtgc cacccgggcc ctgacgttcc
     1801 tgtcagagct gggactgatt acctaccaga cggaatatga cccgcttatc gggtgctaca
     1861 ttccgaccga tatcacgttc acatctgcac tgtttgctgc cctcgatgta tcagaggagg
     1921 cagcggccgc cgcgcgccgc agccgtgtgg tatgggaaaa caaacaacgc aaaaagcagg
     1981 ggctggatac cctgggcatg gatgaactga tagcgaaagc ctggcgtttt gttcgtgagc
     2041 gttttcgcag ttatcagaca gagcttaagt cccggggaat aaagcgtgcc cgtgcgcgtc
     2101 gtgatgcgga cagggaacgt caggatattg tcaccctggt gaaacggcag ctgacgcgcg
     2161 aaatcgcgga agggcgcttc actgccaatc gtgaggcggt aaaacgcgaa gttgagcgtc
     2221 gtgtgaagga gcgcatgatt ctgtcacgta accgtaatta cagccggctg gccacagctt
     2281 ccccctgaaa gtgacctcct ctgaataatc cggcccgcgc cggaggcatt cgcacgcctg
     2341 aagcccgccg gtgcacaaaa aaacagcgtc gcatgcaaaa aacaatctca tcatccacct
     2401 tctggagcat ccgattcccc ctgtttttaa tacaaaatac gcctcagcga cggggaattt
     2461 tgcttatcca catttaactg caagggactt ccccataagg ttacaaccgt tca
//
LOCUS       DAZXUK010000388         2482 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZXUK010000388 DAZXUK010000000
VERSION     DAZXUK010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.391"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>2482
                     /locus_tag="ACH3L8_004118"
     CDS             <1..>2482
                     /locus_tag="ACH3L8_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFT4086984.1"
                     /translation="NERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDVNAYT
                     SYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAHQDAK
                     WRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDI
                     DSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPM
                     VQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFR
                     VNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRML
                     TSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNE
                     LAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTS
                     LTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGVGELS
                     SSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDNIWAS
                     LQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAADRQLF
                     SSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTVKEQN
                     EPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSRLSVD
                     PQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSLDIQQ
                     RSVQQLANTIINSLIQYDDPAAWTEQEQ"
BASE COUNT          725 a          577 c          598 g          582 t
ORIGIN      
        1 acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc acaaaaacat
       61 ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt ggtcgcgatg
      121 ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg actacgcaga
      181 aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc gcaacctttg
      241 aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt gcccatcagg
      301 atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat acccgtaatt
      361 tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg gcacaattgc
      421 gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg gtcggcgata
      481 tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt ccggctaaca
      541 aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc tttaatatca
      601 ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg ccaatggtac
      661 aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta gttcagctgt
      721 tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt tctggcggca
      781 ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt gtaaatgcac
      841 gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca accattgatc
      901 agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc acctggctga
      961 aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt cgcctggcat
     1021 ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa ctttcgaaac
     1081 gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag ttaagaaaga
     1141 accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa aaagcattgt
     1201 ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg gcggcttaca
     1261 tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg gaaattagca
     1321 gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt gccaaggtta
     1381 ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta tcgaataaag
     1441 gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat aaagcagtta
     1501 gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt gcggaaaact
     1561 cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt agcgcgcgga
     1621 ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac agcacgatta
     1681 acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa acgctcgacc
     1741 agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct gatgaccgca
     1801 cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg ctggctgccg
     1861 atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt aatgtcgcag
     1921 aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac tctgattcgc
     1981 cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt actgtaaaag
     2041 aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc cggacacctg
     2101 ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca aaagatctac
     2161 gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc ctctcggttg
     2221 atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca gaacgacatg
     2281 atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag gggatcagtg
     2341 agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc caacagcgta
     2401 gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac gatcctgcag
     2461 catggactga gcaggagcaa tt
//
LOCUS       DAZXUK010000389         2476 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZXUK010000389 DAZXUK010000000
VERSION     DAZXUK010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.392"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(93..1451)
                     /locus_tag="ACH3L8_004119"
     CDS             complement(93..1451)
                     /locus_tag="ACH3L8_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFT4086985.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            1733..1864
                     /locus_tag="ACH3L8_004120"
                     /pseudo
     CDS             1733..1864
                     /locus_tag="ACH3L8_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1929..2059
                     /locus_tag="ACH3L8_004121"
                     /pseudo
     CDS             1929..2059
                     /locus_tag="ACH3L8_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005284207.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2184..>2476)
                     /locus_tag="ACH3L8_004122"
     CDS             complement(2184..>2476)
                     /locus_tag="ACH3L8_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFT4086986.1"
                     /translation="VKLLTEPWGRKMHSDYYNMVFGEKLANILYEANSQFFYERNVIE
                     EAVNALFCEREIINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          616 a          624 c          566 g          670 t
ORIGIN      
        1 gctttcattg ggggtctacg ccggaacccc aatattgttc acatacatga atttgaaaaa
       61 ctaagcgata ctgctgtccg taccttataa acttatttca acgcttccgg cagcgtcaca
      121 atccacagtt cactgtcaga ctccggtttc tccagtggct tcactctgag ggtcgtttca
      181 acctgcttgt cgtcaagcgt cagctttcct tccgcgttca cccgataatc cttgaatgtc
      241 ttgccctcga ccggatgttg catcgccacc ttcacgccaa agtcgttatc gttcgccacc
      301 gccagcgttt tgctgtcgat cagcgccaac ccttcagcct tctcctgctg ccagcccaag
      361 gagcgcagat caacaacctg cgttttctgc gcaagggtaa tgccgcgctg tgacagggtt
      421 ttctcatcat caaactccgg atactcaccc ggcttgtcga agcctgacag gtcgctcgcc
      481 ttattgagat cgacctcgta aatcaggtta cgcattctgt tgtttttatc tcttccctgt
      541 tcgatcagca ggatgtgctg gttatcgagc gccacgatgt ctccgatttt agcgtcgctg
      601 tttttgctgt aggccgcgct gtcgatagga taaccgtaca tcgcggtttt tccgcttgcc
      661 ggatcgaagc tcaccagacg cgtaaacagc gcctttttct tgctcttagc gtcgatatcc
      721 agcgtacttt gcacggcgac aataatacgc ccgtccggca tacgggtgag gccttcaaag
      781 ccgcggttcg cctgacgcca tttgaggata tttggcaaac cgcccgcaat cgctttttcc
      841 ccttccgccg cctgcggacc gtggattgcc aggattttcc ctttactgtc gatgttaatc
      901 aggaacgggc catactcatc gcacagccag taacctcctt taccatccgg agtgatccct
      961 tccgtatcca gcccgcgatt atcccccttc agtctatgta acgtgtcgct gaaggccact
     1021 tcattggtgg agccaatcac gtcgcttgcc agcggcagac cgttgatcgc gcctttatcg
     1081 tcatgcagag gacggggatc gatggcctcc gctttgccgt tttgtacacg aatcgtcatc
     1141 agcagcggcg cgaaatctgg cgtaacgaaa attttagttt cgtttttccc ctctttcggt
     1201 gaatccgcgt tcggaccgcg atcggtaatg gttgcgaatg tcagggcatc gccctgttta
     1261 ccggtgaaca gcaggccaga acctatccca accggaaggc cgttcgggaa tgcactggca
     1321 aatgcgccgg cgtagttcac gtgcgtgcct tctggaaaac tgacgacata gcgctcagcc
     1381 gtaggttgtg ccgccagggc agagaaagag agcgtacaac cgattagcac gggaataatt
     1441 tttcttttca tattgttagc atctataaag tgaagatcac actacaccat aaagaagaaa
     1501 cgagtcagga aaatgacatc gaatggcaac atgcgcgggc ctgaggccaa agaaatgtaa
     1561 aacactgttg ttatttgtaa caacggaaac atttacggaa attatttgtc tttcttcgtg
     1621 atcaaaaatg ctgtgtcttg ttggattgga gtgatgaggg ctgcgtttag gaaacgccct
     1681 atattgtacg gttgcattat cgcggtaggg gtaatgcacg tgtcagtatg atgagctgac
     1741 cacactcacg tgggtggact ggtataacaa tcgacgattg ctggaaaggc agggccatat
     1801 cccaccggca gaagcagaaa aagcttatta tgcttccatc ggaaacgatg atctggcagc
     1861 ctgagttcac agataaaaca ctctccagga aacccggggc ggttcaattg ctggataaga
     1921 tcataacgat acaccgggtt aaacaggtgg tcactgcttc actgaagctg cgtgattatg
     1981 atggtcaggt agcagaagct atgcccatga tacaaaaatg acgaaagccg aaatgtctga
     2041 aagcgtgtgt attacctgaa aatatgaccg gctccggggt acgtaaccga ttcaacaaag
     2101 ccaatcgata ttacaaaacg agagggacta tatatttcgt tttcagataa gactccttga
     2161 gagattatct tttatgtaaa atctcaaata tcacctgatg taatgtcaat gataatatta
     2221 agtgcgctct gaacaacatc tttcttggtg tgattaacat ctgacaaaaa gaacatcagc
     2281 ttctttatta tgttcttgtt gtttattatt tcacgctcac agaataaagc gttgactgcc
     2341 tcttctatga catttctctc gtaaaaaaat tgagagttag cttcatataa aatattggcc
     2401 aatttttcac caaaaaccat attataataa tcactgtgca tctttctccc ccagggttca
     2461 gtcaataatt ttacca
//
LOCUS       DAZXUK010000390         2472 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZXUK010000390 DAZXUK010000000
VERSION     DAZXUK010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.393"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..157)
                     /locus_tag="ACH3L8_004123"
     CDS             complement(<1..157)
                     /locus_tag="ACH3L8_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFT4086987.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYSGHLGLGAGKLYPFV
                     FSYHVPLF"
     gene            608..1798
                     /locus_tag="ACH3L8_004124"
     CDS             608..1798
                     /locus_tag="ACH3L8_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HFT4086988.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(2188..>2472)
                     /locus_tag="ACH3L8_004125"
     CDS             complement(2188..>2472)
                     /locus_tag="ACH3L8_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFT4086989.1"
                     /translation="IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICN
                     CKPANKEQIEKIYPIVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
BASE COUNT          709 a          501 c          527 g          735 t
ORIGIN      
        1 agaatagtgg gacgtgataa ctaaaaacaa aaggatacaa tttgccagcc cccaacccaa
       61 ggtgacctga atatatagca aaaataccga gaaattttaa agcatcaacc caatcatgtc
      121 ttatttttct gttgctgtcg attttgttat tatacattta aagtaggctc tccagttttg
      181 tgtgattttt aaacactcat tgcaaatatg aatgcctgaa agtttatgat tattaatcaa
      241 cgagttcaat attaatttat tatttttcaa agtcgcacta tataggcatt tacttccgca
      301 gtaccaccga tagtatcgat atcaaatcag cctgcggccg cgataacttt cgtaacattc
      361 tgccagcggc tttgttgaat aaatcaggtt caagataatc gtctctgcat caggttagag
      421 tttcaggcaa tacatcctct ttctggcata cctgccactc tcgcaccatg gtcatagcct
      481 ttacgatctg catactcacc agaccagagc ccgctccctt ttaggctgag gaatccccag
      541 aaaagaagac aggcatagag ttttgtgatc tactgattgc gctaacaaat tcaatgagat
      601 cacctctatg cctgcccgta aagtatgcca gacttttttc cgtaatgctt tagcttccac
      661 gcatcagtat cgacaaaatg ccattattga ttctgctgct gctttagtcg gtggcgcatc
      721 tctttctctt accagcattg gccggcattt acccggtcct gctcgcgtga aagataaaat
      781 aaaacgcgtt gactgtctgt tgggtaataa acgacttcat aatgatatcc ctctgatatt
      841 caaaaatatt acatccatga tgacaaataa actgtcgtgg tgtgttatcg ctgttgactg
      901 gagcggttac ccttttcagg aatatcatgt ccttcgtgcc agcctcctct gcgacggtcg
      961 ctctatcccc ctgatgagtc aggtcttccc gtcaaaaaaa aataacgagg cggtggaaat
     1021 cgcttttctg gatgcgcttt ccggggccat ttctccccgg actcgtgttg ttattgttac
     1081 tgatgctggt tttcaaagtg catggttccg tcatatcaaa tcacagggat gggattttat
     1141 tggtcgtatc aggggggtag tgaagttcag gctggatagt gacaaagata agtggctgga
     1201 cataaaaatg tgcaggggga gttcagaggc aaaatacctg gggacgggaa ccctggcccg
     1261 gaaaaaacgc tcgcagtgtg aaggacattt ttatctttat aaacattcac ctaaaggcag
     1321 aaaaagcagg cgtgccagag ggaggccggg cttaccaaca acggaaaaag aacaaaaagc
     1381 cgcaggcaga gaaccatggt taatattcag caatacagct gagtttaatg ccaaaaaaat
     1441 catgaaactg tacagtcgaa gaatgcagat agagcaaaac tttcgggatg aaaaaagtga
     1501 acgctttggt tttggccttc gggcgagcag aagtcggagg ggagagcgtt ttctggtatt
     1561 aagtctactg gttacactgg ccagtatcgt actctggcta cttggttatc attttgaaaa
     1621 taaaggattt catttaaagt atcaggctaa tagtctcaaa aaacgaagag tattgtcttt
     1681 tctgacgctg gctgaaaacg tcctgagatt taaccctgaa cttctacgac gagcacaacc
     1741 tgaaaagata ttgtgccggt tggccagcac ttaccgaagt atggtgctgg cttactgatg
     1801 aaaattttct ggggatccct cagcctttcg gggaggaatg agggctctga tattcttttg
     1861 ccgtagttca tcgtgaaacc gtcggatgtc atttggcctg agtgacccat gcctcccaaa
     1921 agcagagtcg ctagtgttat gtaattaggc ggttaaactt caattctcag tgttaagttt
     1981 gggtaaacgc ttgtaaatgg tattgctaat tttttaattg actaatatta gaaacggtta
     2041 gtgtgattgt atatagatat ttgagtgtaa ggtatttaaa ggggcattca gcccctccgt
     2101 ctatttgtga ttgcatccat tgatgtgtgt attattttga gcttgaaaaa gggaggcgct
     2161 ctttaataat aaataaatct atcaaagcta aatattaaat ggaagccacc cttttttctc
     2221 tgatagtttt attatcacca aaccatttat ttcagctatc gaatctggat tcggccagga
     2281 aggtaaactc ttcacaattg gataaatctt ctctatttgt tctttatttg ctggtttgca
     2341 attacagatt ccatagtaat tcataaatgc aaccatccta aaatagttgc caccatccca
     2401 ccaaaaaaaa gattttccaa aagtgtctgc gctcatggag agcatatttc ctgagtcgtt
     2461 tgaaccatat at
//
LOCUS       DAZXUK010000391         2463 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZXUK010000391 DAZXUK010000000
VERSION     DAZXUK010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.394"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(746..2350)
                     /locus_tag="ACH3L8_004126"
     CDS             complement(746..2350)
                     /locus_tag="ACH3L8_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4086990.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          648 a          576 c          526 g          713 t
ORIGIN      
        1 aagacctata ggtttggtga ttgattatta taaatatcat caccccttaa ggtattccct
       61 tagagttaat ctttaattgg tctttaatta aatcttttat ttaatactta aagttaatct
      121 ctaattgtgt ctttaagtta actttaagta atactataag ttaatcttta attgtgtctt
      181 taagttaact ttaagttaag cccctgtagg gactagagac cataattaag atgattgtgt
      241 cttattgcta ttcacttatc gactgaccca gagcacacag gaagcattca ctttaagtag
      301 aacgcttggt gtctgtaatt ggtcatcccg cccctgaaag gatgcgataa gttacggtta
      361 gttgtcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      421 tccttgacaa gtagagaaaa ccatgagtca aaaacatgac cacacaggtc tgtagtaagt
      481 gtcatcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      541 ccccttgaca agcagagaaa actatgagtc aaaaacatga ccgcacaggt ctgtagtaag
      601 tgtcatccta gaagagaaaa ctttttgtgt cccttctccc tatagtgagt cgtattaatt
      661 atcccttgac aagcagagaa aactatgagt caaaaacatg accacacagg tctgtagtaa
      721 gtgtcatcct agactatggt ctcctctagg tctccttata aacatcagcc atcacattga
      781 ccagcctatc aacctcgacc tctttagcat aacggtcgta cgtaatagac cctgaactat
      841 gccccatgat cgcctgtgca tacgccaact cgaccccatg ttgttttaac tgggccgcaa
      901 ctgtatgtct aaacgagtga aacgctaagc cgctacggtc tacgaggact ttaggcaaca
      961 acgtccgatt aaaccattta ctcacctgct caccgtaacc gttcttcatg agacgcagac
     1021 catcgaacac catagcgtca tcaccgtcag cgcctctacg ggtctctaca agcgccataa
     1081 agtcagccaa tataaaacca taggccccat cgaccagagg cacacagcgg tcactatgag
     1141 cgttcttgat gctcttaccg ggtagactac tatcgtcttc attgatatga atataaacgg
     1201 ttcccgcctc agtggtcctg acgtctttga cctgtagttg ggctatttca ttgagtctcg
     1261 ccccagtgat agcagccaag gcggtaacat aataatgata cggcttacca gaggtcttct
     1321 gagagtatgc cttagctgcg accagtagtt gccccacctg ctccgtagag aaggcgttac
     1381 gggcccctga ggctttacgc tgagggactt taagctccag accctcagtc atgttctttt
     1441 taatcaagtc attgcgcaca gcccacctaa agacagcagc catcttaata agatacttgt
     1501 tgttgatggt aacgacatcc agacaatctg tcttatcttc acggctcaac aggtcagcaa
     1561 gtgggacatc cttgaagcgc tgcttacggt tcctaggtaa ctgctggagc acatcacgga
     1621 ccctaagcat gtcagccctg ttagcgtctg cattgagacc cagatggtcg aatatttcga
     1681 ttaacgcagc gtgtgacgcc ttgttttccc ttaaggttgc aggcttccag ttctgatagt
     1741 tctccgcttc gtattgttca aagaggctcg ctatggtaat gctaggcgta acttctggtt
     1801 ttacttctgg agcattaaca gatagggaca cagagtctgc ttggttatct tgcctatcaa
     1861 tatcattaaa aagatcgacg taatctatca gaggctgact gtctcccgct tcgatacgcc
     1921 tcatacaggc ttttagcacc ttcagggcct cattgatgta acgatgctgg tcaatgctta
     1981 gcggctcact agccagagca tcggtaaggc tctcaccaag ttctccgtat tggtcccggt
     2041 agatatccct catgtcaggc tcaaagagat ctgaacggcc catagacagt tcacactcag
     2101 cgatgtcctt aaggtgttcc ctcatttcct cataggtagc gttgggtctg tctgcgtgta
     2161 ttgccttgag tgctgccttt atgtgccttg agtaagccat agcggtgcgt ctatccttgg
     2221 tccgtaatga tattgaggtc attctatcat tgtttgattg tctgacccta agacggaagt
     2281 agtagacgcc gttacgctgg tagagatagt agactgctgg aagtttgtga ctactggata
     2341 gctgaatcat atgtctgcat gccctgagag ggttacacag tgggttacag taagtaactc
     2401 tgcaaggagg cttaaagccg tatcccttag gattcagtag gttggagtac tttaagaaag
     2461 gtc
//
LOCUS       DAZXUK010000392         2448 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZXUK010000392 DAZXUK010000000
VERSION     DAZXUK010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.395"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(144..2411)
                     /gene="adiA"
                     /locus_tag="ACH3L8_004127"
     CDS             complement(144..2411)
                     /gene="adiA"
                     /locus_tag="ACH3L8_004127"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFT4086991.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          561 a          600 c          670 g          617 t
ORIGIN      
        1 aaaaaaagcg tggggtgggg ccgcgcaaaa agtatgacat ttttgtttgc agcaattttt
       61 atgaaaacgt tgccatactc aaaaggccta aggccaggcg gcgtaatgtt atttaaacaa
      121 ttacgccttc agcggaatag tggttacgct ttcacgcaca taacgtggta aataccgtca
      181 ataatttcag tcccttcagt ttcgtgttca aatctaggga aatggtggtc ccaggattgc
      241 agcgagcgta aataacttac ttgcggactg tttttatcgc cgaagttttc accagacagc
      301 agcatcggga ttcctggcgg atacgggata actgagtttg ccgcgatgcg tcctggcaga
      361 ttttcaatgg ataccagttc gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg
      421 gtgatttccg ccaccggcag gccggaatag gcttcgttca accgtgcgcc agggttgttt
      481 tctttcagcc aggcaaacat ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg
      541 tcaggatatt gttcaacaag ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag
      601 tggtgtttga aggagcaaag ggtgttaacc agagtccccc atttcccacg ggttacgccc
      661 atagagaaca ggaacataat ttggaagtca gtggtgcggg taggcacaat gccgtggcga
      721 ccaagccagg cagtgaccag cgccgccgga acaccggttt cttccagttc accatcttca
      781 cccattcccg gagcaaggat gctgacttta attgggtcga gcatactcca gttatccgga
      841 atatctttga agccgtgcca gctttcgccc ggatgcatta cccagcagtc ctgaacggtg
      901 gtcagcagtt tggttggtgc gtcagcaaag tcataggttt tgccggtttg tgggtcggtg
      961 acgacttctt tgttccacgg tttgaagaac cagctaccgt cagcggtgaa ctctttatat
     1021 agccgcgcca tcgcctgacg gaaatcaacc gcttcgtcaa tcacttcctg tgtcagtgac
     1081 aggccgctgt tgccgtccat catcgacacc gccacgtcgt tggatgcgca gatggcatac
     1141 agcggggagg tggtggcatg catcatgtag gcctggttga agcgggagaa gttaatcgcc
     1201 ccacgacctt cacgtacatg aatataagaa gcctgtgaca gcgcattcag cagtttgtgg
     1261 atggagtggg tggcgaaaac ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca
     1321 tagtgatcgg catagatcgg gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc
     1381 agacgatcgg aggttttttc cagcagatcc tgcgcttctt tagcgttata acacacgccg
     1441 tcataggtgc agttggttac cacgcagtaa gacggttttt gcccggcttt gtctttggtc
     1501 agcgggcttt cactgatttt cttttgcaag gtttcaggtt gcatttcctg cggatagatt
     1561 ggcccgataa tgccgtagcg gttgcggctt ggcaccatat agaccggttt cgcgcctgtc
     1621 agcatcaaac cttgttcgat ggatttatgg cagttacggt caacgaccac gacatcgtta
     1681 tcggtcatgc aagcctgcat gatggtgcgg ttagagccgg aagtaccgac gactaccgac
     1741 caggagcgat cggcaccaaa tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca
     1801 gtatggtcaa gcaaagaacc gagggaagtt cgttcgatgc ccatgtcggt acggaacaga
     1861 ttttcaccat agtagtcatg gtagaaacgt ccggcgggtg ttttggtaaa accaacgccg
     1921 ccctggtggc ctggcgctgc ccaggaatat tcatggatgt cactatattt catcagcgcg
     1981 ctgaacagtg gcggcaacag ctgctggcgg tagcgggtca tcgcggcaac ggcgcgtccg
     2041 gcgataaagt cggcggtatc ttccagaatc caggcgaatt catcgacaag ctccagcaga
     2101 tcccggtcca ttgcggcgag ggctttttcc cgatcgccca acaggaagac cggcacgttt
     2161 tgttggcgct catgaagctt accgatcaat tgtctgacgt tttgatgttc gtccggatgc
     2221 tccatttgat agctgaacat caggcagtca atggcttcgt ttgaagagag aatggcaaaa
     2281 ccatcatcaa aggaggtgga tttaatcacg gtaacatttt gctggcttaa agcatctgcc
     2341 agacgctcaa cggcgttacc gacccaggtg tcttgatgga gaaactcgct ttcaacaatt
     2401 aatactttca tcattgctta cccggttatg aaggaatctt cgttgcgg
//
LOCUS       DAZXUK010000393         2445 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZXUK010000393 DAZXUK010000000
VERSION     DAZXUK010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.396"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..673)
                     /gene="chbR"
                     /locus_tag="ACH3L8_004128"
                     /pseudo
     CDS             complement(1..673)
                     /gene="chbR"
                     /locus_tag="ACH3L8_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(681..1031)
                     /gene="chbA"
                     /locus_tag="ACH3L8_004129"
     CDS             complement(681..1031)
                     /gene="chbA"
                     /locus_tag="ACH3L8_004129"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFT4086992.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1090..2438)
                     /gene="chbC"
                     /locus_tag="ACH3L8_004130"
                     /pseudo
     CDS             complement(1090..2438)
                     /gene="chbC"
                     /locus_tag="ACH3L8_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
BASE COUNT          685 a          629 c          528 g          603 t
ORIGIN      
        1 tctcattaat aatctgcatt ggtgttttgc cataatatcg ttgagtcgct cgcgtcaaat
       61 attcctgtga tttggctgac aacgtcacca tattctccag cgccgattca ctaaactgct
      121 ctttatcatg catcttttct accgtacttt tcagccacta cggtacatca tcaatcacct
      181 gttcttcgcg gtaatgacgt aaacggttaa tgacataaaa agtaaccatc tcaacaaact
      241 cttccagccc tgtttcgcgg aaatttaatg aagaaatcac tgtttccaca taggtgagaa
      301 aagcgttatt ggtccggtat acctgcgaag caacaaagca ataaggcaac aacggcaggt
      361 aatgctgctc aaaaaagcgt ttactgatcc caacgttcaa tatgcgcgtg gcaccaaact
      421 cataaaaact ttgatggtgc gaacctaacg gaataaaaac aaaatcgccc cgttccagta
      481 acacgcgctt accgttaatc tcctggaaat aacgcccggt taataccaga gtaaattcat
      541 aatagtcgtg ctgatgcagt ccgctgatac tctcagtttt gttatagata aacacatgga
      601 agtttttgcc attaaacaac tgctgttctc gggcagtggc aatttccggc gcgttaatca
      661 ctggctgcat catcgactcc ttatgccttc agtttttcat gaagctcaat taattcagta
      721 atcagttcac gcgcaagcat ggacgtcatt aaatgatcct gagcgtggac cagcaccaga
      781 ctcactttca tcttaccttc gcccgcatcg ccttcaatca gtttcgtctg taccagatgc
      841 gcttcattca atgccatccg tgactgatcc atcatggctt tcgctgcggc aaaatcgccc
      901 tgcttcgcct gtttcaatgc ggcatacgcc aggctacgcg cttgtccaga gttgatgata
      961 agccccatca ccacttcttc cagctcttca gcttctgttt gcgtatcagg aatattatcg
     1021 agatccatca tacatcgttc ctctctcttc ttaccggcaa gattacccgt accggcatcg
     1081 attaaaattt caggggttag cgatatcttc ttcgctctct tctttatcaa tcgcattttg
     1141 tgctttgtta gccaccacaa caaagggcag ataaattaac gttgcgatgc caaggttgaa
     1201 gagtgcgacc agcaatgcgg cgacgctacc gttggtgtta aagaaggctc ccagaccggt
     1261 tggcatggtc cacggtgcaa tattggtcac cggaggaatg atgcccatgt agtacgctgc
     1321 gagggtgatt gccgccagaa tcggttgtac cagtacaaac gggataaaca tcaccgggtt
     1381 catgataatt ggcagaccaa acagaatcgg ttcattaatc tggaagatgc cggacggcag
     1441 cgccagtttt gccacctgac gataatcagc acggcgagag gcgataaaga tagccaggat
     1501 caggcctaaa gtcgcaccac tgcccccaag gaaaataaag gagtccagca tcggcttggc
     1561 ccagatatgg aaggtcttac cggctgccag cgccgcttcg acggaaccat attgctgata
     1621 ggtcgcgata ttttccagcg cccacggcgt cataatgccg ttgtccagtg cggtcagcgc
     1681 cagcgcgcca tgaataccga agaaccagag cagtggaaca aagatcacat aggcccagcc
     1741 caccacgcta cccaacgatg ccagtggggt tgagatggta tccataatga tctgatggaa
     1801 gttggtgccc caggtattca acgcccaggc aataatcccc atcacggaaa gaataataaa
     1861 gccgggaatt aatgccgaga aagaacgcga tactgaagca ggtacgctgt cgggcagttt
     1921 aatgacccaa ttgcggcgga caataaaggt aaacatttct gccaccacca ggccaataat
     1981 aatcccggag atgatattcg ccccacctaa ccagtttgcg ccaaccgcat aggcctcacc
     2041 gacactatat ggggtgacgg tcataaatgc tgcaacggat aacaacccag ccgccagcgc
     2101 atcgacttta cgctcttctg ccagcgccat gccaataaag aacggtgcca ttaaagacat
     2161 tattcctaat gttccgttat atacgttgcc gccaatacct ttcagaccat taagtgtttc
     2221 aatggttgag gcgtcgaggc gaatacctaa ggaataaaaa acgacccctc cccaaagctt
     2281 agaaaaacgt tgttaattaa tacaaacatg gcccccgcaa gggttaacgg cattaagcga
     2341 ataaagccat ttttgattgc attaacgtgt ggctgctttc ctattttaac tgcaaaaggg
     2401 aggagtacct tttcaagcga tgcaataaca ttactcatag aaaaa
//
LOCUS       DAZXUK010000394         2434 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZXUK010000394 DAZXUK010000000
VERSION     DAZXUK010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.397"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..360
                     /locus_tag="ACH3L8_004131"
     CDS             <1..360
                     /locus_tag="ACH3L8_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFT4086993.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="ACH3L8_004132"
     CDS             361..726
                     /locus_tag="ACH3L8_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFT4086994.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1411
                     /locus_tag="ACH3L8_004133"
                     /pseudo
     CDS             723..1411
                     /locus_tag="ACH3L8_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
     gene            1605..1680
                     /locus_tag="ACH3L8_004134"
     tRNA            1605..1680
                     /locus_tag="ACH3L8_004134"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="ACH3L8_004135"
     tRNA            1682..1758
                     /locus_tag="ACH3L8_004135"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
     gene            1761..1836
                     /locus_tag="ACH3L8_004136"
     tRNA            1761..1836
                     /locus_tag="ACH3L8_004136"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1794..1796,aa:Thr,seq:tgt)
     gene            1840..1914
                     /locus_tag="ACH3L8_004137"
     tRNA            1840..1914
                     /locus_tag="ACH3L8_004137"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1873..1875,aa:Gly,seq:tcc)
     gene            2207..2422
                     /locus_tag="ACH3L8_004138"
     CDS             2207..2422
                     /locus_tag="ACH3L8_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFT4086995.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          639 a          520 c          653 g          622 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtaggt agagcaactg acttgtaatc
     1801 agtaggtcac cagttcgatt ccggtagtcg gcaccatatg cgggcatcgt ataatggcta
     1861 ttacctcagc cttccaagct gatgatgcgg gttcgattcc cgctgcccgc tccagtcaga
     1921 gtctttcagt ctgcgatgat gggaaatccc ggagtgactg aaagacgttt aagttatgaa
     1981 tgatcgcttt tttttgcaaa attgctgtgc agaaatacta accttcgggc aggcgatcat
     2041 tcataagcac tctgctttta ttccgattaa ctgtgagtgg tttgttggat agagtgcttt
     2101 ccttattgta tatatcgttt cgcccgcttt tgcggttttt tcttttcaaa tccctttcat
     2161 ttctcagtgt aaaactacgc catccgttat ttgcggaggt gaggctatga aatccatgga
     2221 caaaatttca acgggcattg cctacggcac ctccgcaggc agtgctggct actggttttt
     2281 acagtggctt gatcaggtca gtccgtcaca gtgggctgcg attggtgtac tggggagtct
     2341 ggttctgggc ttcctgactt atctgacaaa tctgtacttc aaaatcagag aagacaagcg
     2401 taaggctgcg agaggtgaat aatgtcgcca tcat
//
LOCUS       DAZXUK010000395         2430 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZXUK010000395 DAZXUK010000000
VERSION     DAZXUK010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.398"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..712
                     /locus_tag="ACH3L8_004139"
                     /pseudo
     CDS             1..712
                     /locus_tag="ACH3L8_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2318..>2430
                     /locus_tag="ACH3L8_004140"
     CDS             2318..>2430
                     /locus_tag="ACH3L8_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFT4086996.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAV"
BASE COUNT          780 a          441 c          528 g          681 t
ORIGIN      
        1 tcgagtaata atgaatatga cctgtttaaa aatagagaaa cctcactgtg gaaacgcaaa
       61 gtaccagaaa gcgcagcgct ggcaatgacg ggaacagtat ataaattatt tggtaatcgc
      121 ttactcaacg cccgcaatca gttagataaa aatctcctcg aaatggctat gcaaatggtt
      181 tatggcaaag atatggtgac aacgtccggg gatatattga ttgatataca attaaataag
      241 gatggcagtc tccagagtaa gcactacaca cttgatgtag gtgaagttat aaacagttat
      301 aatattgacc ctctttgtga atgcttcaag tattaatcaa acttacttac atgataaaaa
      361 tcctggtgac gttattagcc tgtatggcac tttagataac attccccttg tagttaagga
      421 atgcacgggt aaaaatcatc aaattaatgt taaagccaaa ttgccacaag aacgaagtga
      481 aaaagttgag gatatgtgtg aaagaatgaa aggtggcatc tctatgttta accacaccac
      541 aaaaacagct gataatatag agcataattt gcagctaagt tttctcgtgg gttcacgtcc
      601 gtcgatcacc aataaatact cagcaaaaga tgtaggtcca gatatacttg cattgcccgt
      661 caacgtatat catgcggatt ttaaaattaa aatcaataat gcagtggaga aggcttcatc
      721 aattgtgtta tgtgctgtgc aaaaaaatct ccatgcagca aggaaggttc ttagcgggat
      781 aataggaaat gtgccaaaaa ataataaatc aaaaacaatt atttttgatt tgcgttttct
      841 tagtgcaatt aagcagaaag tttttttggg cagtgaaaaa ccgttgtttg taaagcatac
      901 cgcaatgatg agggaggcat gtaaggaatt accgcagtca gtggaatatg ttcctttagc
      961 ctgtgaagcg catagccatc gcagcgtcgc ccttgccaaa accgtggctg cgcccatcat
     1021 ggcgacaggt gtgggcttgg cattaatgta tgcaacatca tcaggaccgt attatgcgca
     1081 aagtctttct ggttcgccgg atatcgagat gataaaaaat cgcatggctg aattgtttaa
     1141 ccagtcggat atttctgtgc gcaacaaata tagcccggct tttgataaat ggaatgaact
     1201 ggtagataaa ttaaatcacg aaagaaatac agttccgttt gcgtgtttga ccaccatttt
     1261 agcgacggcg ataaatgaaa ctcttgaggg ggatacgaat gttgccgttg tgatggggtg
     1321 taaatctgcg aaagatcgca cgatatccat tgtccttggc aactcgatgt tgcaaacact
     1381 ccttgaaaaa cgcctcgccg atagtggaga aatagaaaat ctttttgatc aacagggata
     1441 ttttagttgc gattcattaa cacccgaaga attaatgatg ttaaaagacc tttttgatat
     1501 tcgagttctt catgtctcta acaaattcaa tgttggattg caaggtaata tcaacactga
     1561 tgttttacaa gattcgtttt ttaaaaatgt cgatttcatt catgaatata actcgtatac
     1621 cgtagggatg ggagtctgac gagtaaaact cattaggatt atcagaattc ggacaatgca
     1681 ttgcaaaaat gacatacaaa gagagatgtg tgcaggtact tagccgcgag gggcatggcg
     1741 aagccataca gaaagtacca gtattcagaa ctccagtgaa agcggcatag tggccgcctg
     1801 ctcaggagtt ttgctatgac ccgtaatcgt gctgaacgtc gccaccctat gatgcgatta
     1861 aaaaaactca tcgtcgcgct gatgattgac gtcaatgcta tcggatgatg ccgggggtgt
     1921 tccggaagga attccttgtt tcccgaactg ttaccaacaa aattataacg atgagcaagt
     1981 aaagaccggt tggtggcagt actgaccggt ctgtgatgaa agattaattt caccttaaat
     2041 cctggtgctg ttaggatgct gacgtttttc ggctactcca ttcagattat gtaaataaat
     2101 atccctgaat ataatatatt catagcaccc ataactcatc ctgggcgata actaaaccat
     2161 ttttgagtaa taaatgaaac aactgcaacg ctcttattat ttctggaaaa cgcctcgcaa
     2221 aaacaaaatg acatgcaaaa atccgcattt ttttaaatgc ttttccggcg taattttcgc
     2281 acatacatta atgagtaata tatgtaaggt ggtggtaatg gcgatgaata cggtttttct
     2341 tcatttatca gaagaggcaa ttaaacggct gaacaagctt cggggctggc gtaaggtttc
     2401 acgatctgca attttacgcg aagcggttga
//
LOCUS       DAZXUK010000396         2427 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZXUK010000396 DAZXUK010000000
VERSION     DAZXUK010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.399"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1393)
                     /gene="mxiA"
                     /locus_tag="ACH3L8_004141"
                     /gene_synonym="virH"
     CDS             complement(<1..1393)
                     /gene="mxiA"
                     /locus_tag="ACH3L8_004141"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFT4086997.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSY"
     gene            complement(1406..>2427)
                     /gene="mxiC"
                     /locus_tag="ACH3L8_004142"
     CDS             complement(1406..>2427)
                     /gene="mxiC"
                     /locus_tag="ACH3L8_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFT4086998.1"
                     /translation="KLNAMTNSDDGDETADAELDSGLANSKYIDSSDEMASALSSFIN
                     RRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLPLDRDFIDRLKRYFKDP
                     SDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAGINSAIQAKLFGNKMKL
                     EPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFVEQSLIVDMDSEKPSCN
                     AYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAEQLLLTLLYIFQYPSES
                     EQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESEREIAINILRELVTSAYK
                     KELSR"
BASE COUNT          839 a          503 c          330 g          755 t
ORIGIN      
        1 tatatgaaac gtctacccaa gaaataacac gttcattata tgaagttgaa acaacaggaa
       61 tacctagagc atctatatct ccattttcat ctgtaataca cactttgtca aaatatatat
      121 taaaactatc ggcacgcacc tcatttatta acaaaacaat atcatcgacc ttaagctcat
      181 tgcttgttct atataaaata gttggaagcc tgacaccata atcaataaag aactgacttc
      241 ttatcctttc aataagacct tccatatcat ttgcatttat cttattttcg gcaaataata
      301 aaattaaagg aacggtttct gaactaatag catccagatt ttctatcatt gccaaagatg
      361 agtcatggct attatcaata tcaaatgtac cagtatagcc actagaatca gactcagaca
      421 gacttttttc tttttctacg acctttttat aatagaataa agccgtcaaa gtaactgcta
      481 tcaggaaaaa aacaaaaaac ggaaaacctg gcaacatccc tatcgccaag gcaagagctg
      541 atgtaacaat gagaacaaaa ggattcccaa atatctggga cattatgtta cgtcccatat
      601 tatcgctatc accattaact cttgtaacga taaatcctgc acttatagaa attaacagag
      661 caggaatctg agagaccaat ccgtcaccaa tagttagtat ggtataagta gataatgcac
      721 cagataggga cattccatgt tgactcatac caacagaaat acctcctatt aaattgacaa
      781 aaataataat gatgccagca attgcatcac ctttaatgaa cttcattgct ccatcaaatg
      841 aaccatacaa ttgactctca cgttccagaa tactgcgtct ttctttagct cctgcggcat
      901 caataattcc agcctttaaa tccgcatcaa tgctcatttg tttccccggc ataccgtcaa
      961 gagaaaagcg agcagctacc tccgcaaccc tttcagatcc ttttgttata acaatgaact
     1021 gcacaacagt tactattgaa aaaataacaa aacctacagc cagactatca ccaatgacaa
     1081 actggccaaa tgtagtaata attttacccg catccgcatc aactaaaatt agtcgactag
     1141 tacttattga taaagcgaga cgaaataatg tcgttatcaa caaaaccgaa gggaatgtag
     1201 aaaaactcaa tattctttca atataaaatg atcccataaa aacgagaata gcaagtacaa
     1261 tattaagccc tattaaaaaa tcaacaaggt atgtaggtaa tggtatgatc aacatagcaa
     1321 tgatcataac cataagcact aatataatta attcaggctt agtacttact tgcttaagaa
     1381 aagactggat cacttttatc tcctgttatc tagaaagctc tttcttgtat gcacttgtga
     1441 caagctctcg tagaatattg atcgcaattt ctctttcact ttcactttta aatatatcat
     1501 gaggactttc attaacagaa gatagatatg tttgatacac tacagaatcc tcatgactgg
     1561 ctcgtgatac ttctataaca gaagtaagaa tttgctcact ttcacttgga tattgaaaaa
     1621 tatataataa agttagcaat agctgctctg cacttaaact gccatctttt agcaagcttg
     1681 aggattccag tttcttcata aaaattacgt ctgaagttct aatcatctta attgcaatta
     1741 atttagataa cacaaaacca aactcataag cattacagct cggtttctca gaatccatgt
     1801 ctacaatcag tgactgctct acaaaattca caattgtatg tctgtgatta aaaccaaaat
     1861 taccaagcca ttctatatac tgatctgttg ttgatatgtt ccccatgata aaaccacgat
     1921 aacatgcacg caaaagctgt ggttcaagtt tcattttgtt gccaaataat ttagcctgta
     1981 tagctgaatt tattccagca ttaacacttt tttcactacc tgatattatc tcattaataa
     2041 ttttagttaa taattcgact tgttcagcag taagatcttt ttcattcaaa agttccctta
     2101 atgctaagac ttgatcactt ggatctttaa aatatctctt tagtctgtct ataaaatccc
     2161 gatctagagg aaggttatct tttaacgttc tctttaaatc aaaaatcttg tgattaattt
     2221 catcttcttc cccatctaaa atacgttcct gactatcact atttgttcct ttcagtttct
     2281 caaggtctct tctgtttata aatgacgaaa gagcggaagc catctcatca gatgagtcaa
     2341 tatacttgct attagccaag ccagaatcaa gctctgcatc agcagtctca tctccatcat
     2401 ctgaatttgt cattgcattc aacttat
//
LOCUS       DAZXUK010000397         2325 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZXUK010000397 DAZXUK010000000
VERSION     DAZXUK010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.400"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            61..282
                     /gene="kilR"
                     /locus_tag="ACH3L8_004143"
     CDS             61..282
                     /gene="kilR"
                     /locus_tag="ACH3L8_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFT4086999.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            282..452
                     /locus_tag="ACH3L8_004144"
     CDS             282..452
                     /locus_tag="ACH3L8_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFT4087000.1"
                     /translation="MTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="ACH3L8_004145"
     CDS             527..802
                     /locus_tag="ACH3L8_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087001.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="ACH3L8_004146"
     CDS             902..>2325
                     /locus_tag="ACH3L8_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFT4087002.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          719 a          531 c          529 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatgttcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DAZXUK010000398         2297 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZXUK010000398 DAZXUK010000000
VERSION     DAZXUK010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.401"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            198..377
                     /locus_tag="ACH3L8_004147"
     CDS             198..377
                     /locus_tag="ACH3L8_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFT4087003.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            463..963
                     /locus_tag="ACH3L8_004148"
     CDS             463..963
                     /locus_tag="ACH3L8_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFT4087004.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            1009..1515
                     /locus_tag="ACH3L8_004149"
     CDS             1009..1515
                     /locus_tag="ACH3L8_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFT4087005.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1575..2213)
                     /gene="ompW"
                     /locus_tag="ACH3L8_004150"
     CDS             complement(1575..2213)
                     /gene="ompW"
                     /locus_tag="ACH3L8_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFT4087006.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT          728 a          503 c          488 g          578 t
ORIGIN      
        1 gtaaaatcaa taattttctc taagtcacat attcctcagg taattgttaa tatatccaga
       61 atgttcctca aaatatattt ccctctatct tctcgttgcg cttaatttga ctaattctca
      121 ttagtgacta attttaatga gtgtcgacac acaacactca tattaatgaa acaatgcaac
      181 gcaacgggag aaataacatg gccgaacatc gtggtagttc aggaaatttc gccgaagacc
      241 gtgagaaggc atccgacgca ggccgtaaag gcggtcagca tagcggcggt aattttaaaa
      301 atgatccgca acgcgcatct gaagcgggta aaaaaggcgg tcaacaaagc ggtggtaata
      361 aatcaggcaa atcctgattc accgttaatt attaatatca gctgataaag ataattgcat
      421 ctgcgggccg tatggctcgc agtgatttca ctggagaaaa atatgaatat gaagaccatt
      481 gaagatgtat ttattcaact gctttcagat acctacagcg cagaaaaaca attaacccgg
      541 gcactggcaa aactcgcaag agcaacatca aatgaaaaat taagtcaggc ttttcatgcg
      601 cacctcgagg aaactcatgg acagattgaa cgaattgatc aagttgtgga atcagaatca
      661 aatctgaaaa ttaagcgcat gaaatgtgtg gcaatggaag gtcttattga agaagctaat
      721 gaggtcatcg aaagtaccga gaaaaacgaa gtgcgtgatg ccgcactgat tgccgcagca
      781 cagaaagtcg agcattatga gattgccagt tacgggacat tagcgacgct ggctgaacaa
      841 ttaggttacc gtaaagcagc gaagcttctg aaagaaaccc tggaagaaga aaaggccacc
      901 gacatcaaac tgactgatct ggcccttaat aacgtaaata agaaagccga aaataaagcc
      961 tgaaatatga attttaactt ttagtcattt tataaagagg acattttcat gaatcgtatt
     1021 gaacattatc atgactggtt acgtgacgcc cacgcaatgg aaaagcaagc cgaatctatg
     1081 cttgaatcca tggccagccg tatagataat tatcctgaac tacgcgctcg tattgaacaa
     1141 catcttagtg aaaccaaaaa ccagattgtt caactggaaa ctattcttga tcgtaatgac
     1201 atttcacgtt cagtcattaa agattccatg agtaaaatgg ctgcgcttgg gcagtcaatc
     1261 ggtggtatat tcccttctga tgaaatagtt aaaggctcta ttggcggata tgtcttcgag
     1321 caatttgaaa tcacctgtta cacctcacta ttagcagcag caaaaaatgc cggtgataca
     1381 gtctcaattc cgatcatcga agcgatttta aatgaggaaa agcaaatggc cgactggctg
     1441 attcagcata ttccgcaaac aactgagaaa tttttaattc gttctgaaac tgatggcgta
     1501 gaagcgaaga aataataccc tttataccat gtccttattg accccgtata ttacggggtc
     1561 gtttttgtgc ggaattaaaa acgatatcct gctgagaaca taaacaccca cggatcgagg
     1621 cgtacgctat cgtgttgctg tgcaccgcct agcttataat tggcggtggt atcgatatcc
     1681 atgtaccaca ctgacatgtt aaccaaccag tcacggttaa tcagataatc aacccccacc
     1741 tgcccggcag ctccccagga atctttcaga ctgagatcgg aaagccccgc ctctttgcca
     1801 tgatcgttaa atccattatc aaagaaggtg gtgtagttaa taccggcccc aacgtaagga
     1861 cggaatttgc tgctggcatc accaaaatac cactgcgcca tcagtgttgg cggcagatgg
     1921 tgaacggttg caatatcgcc ggtcgcccgg gtgccgattt tatggcggaa cggcgtcgct
     1981 gccagtaatt ccacaccaat gttgtcggtc gccatataag taaacgtcaa gcccagttga
     2041 gtgttattgg tcacgctgaa tccacccaga cttcctaacg taccaccagc accttctgtt
     2101 ggacgtacgg ttgcagaacc tgcacgcata aaaaattcgc ctgcttcatg cgcaaaggca
     2161 ctgccagaaa gaagagttgt tactgccaaa gccgccactg ttaacttttt catatccgct
     2221 ccgtcgttat ggttataaaa acgtgacgaa tatacctaca ttggagtaac aaatgatcct
     2281 acatagatca cattaca
//
LOCUS       DAZXUK010000399         2279 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZXUK010000399 DAZXUK010000000
VERSION     DAZXUK010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.402"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..796
                     /locus_tag="ACH3L8_004151"
                     /pseudo
     CDS             1..796
                     /locus_tag="ACH3L8_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            911..1252
                     /locus_tag="ACH3L8_004152"
                     /pseudo
     CDS             911..1252
                     /locus_tag="ACH3L8_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1235..2072
                     /locus_tag="ACH3L8_004153"
                     /pseudo
     CDS             1235..2072
                     /locus_tag="ACH3L8_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2122..>2279)
                     /locus_tag="ACH3L8_004154"
     CDS             complement(2122..>2279)
                     /locus_tag="ACH3L8_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFT4087007.1"
                     /translation="ALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGT
                     FYTPQGS"
BASE COUNT          553 a          554 c          692 g          480 t
ORIGIN      
        1 gggccttgcc cgtagcagtt atttttatca ccgggctgca ctgaaagccg gtgataaata
       61 cgccacgata cgtaagatgc tgacagatat ctttaacagt aattaccagt gttatggcta
      121 tcgtcgcctg catgcgatgc tcaggcatga gggggggcgg ctatcagaaa aggttgtacg
      181 cagacttatg gtggaggaac agcttgtcgt cagtcgtaac cgtcgtcgtc gctacagctc
      241 atattgcgga gaaatcggac cggctccgga taaccttatc gccagagatt ttaaggcgga
      301 gcaacctaat cagaaatagc tgacagatat cacggagttc cagctccctg caggtaaagt
      361 ctggctgtca ccagtggtgg actgcttcga tggaaaagtt gtgagctggt ctctcagtac
      421 acgccctgat gctgaactgg tcaacactat gctggatagc gctgtcgaaa cgttaaatgc
      481 tggcgaacga ccggtgatac acagtgacag aggtgggcat tatcgctggc caggctggct
      541 ggaaagagtg aatgcagcag gtcttattcg ctcaatgtcc cgtaaaggat gttcacctga
      601 taatgctgca tgcgaaggct ttttcggcag actgaaaacg gaaatgtatt atgggcgtaa
      661 atggtcgggc atcacgccag aaaagttcat gcagcaagtg gatgcttaca tcagatggta
      721 taacgagcgg cgtataaaat tatcgctggg tgccgtcagc cccaaaatgt accgccaaca
      781 atgcgggctg gaatgataaa gcagtccagg aaatcgtccg catcccccct tcaccatttg
      841 gattgtccgc tccacccaac atgttgtttc cttaaggttc ctacaccaca aaggaaactc
      901 tcatgctgcg cagtggatgg ctgacgttgc cgacaggcgg gaacttcgcc agttcaggca
      961 gacaccggaa cagcgcttca cgcaggaaca ggagcatctg cagccgctgc tgggtacgga
     1021 cttcgatatc cgtcatgtct cctgggatgg ctatatcgag gttggcggga accgttacag
     1081 tgtgccggaa agcctgtgtg gtcagctggt atcaatacgg atctcgctgg atgatgaact
     1141 gcggatctac agtaatgagc aacaggtagc atcgcaccgt ctctgttcgg ctgcatacgg
     1201 ctggcagacc gtgccggagc atcacgctcc actctggcag caggcttcgg agcgcatggc
     1261 cgaatggctg ggggagatac agaaacgggt aatcactgtc tgtgaccggg aagcggatat
     1321 ctggcattac ctgtattata aagtcagcca cggacagcgt ggtgcgtgct gcacagaatc
     1381 gccggctgga agaggcaccc ggcaagctct tcgaactgcc ggaagtcctg gcaaccgcag
     1441 gaagccacac gctgaatgtg atgcaaaaag gcgggcgggc agcccgtcag gcccggatgt
     1501 tcatcagtta cagcgaagtc agcataaaaa atcccgacaa cagcggccag gcgctcccgc
     1561 tcacgtatgt ctgttgccgg gagcaggcag aggacggtgc ctgctggcat ctgctgacgt
     1621 caggaaaagc ggcgagtgcc gccgatgcac gacgtattgt cagccattac gagcgacgct
     1681 ggctgacaga ggaataccac aaggcgtgga aaagtggtgg tacatggaat cgctgcgaat
     1741 gcagacccgg gataaccttg agcgcatggt ggttatcctg gcgtttatag cggtacgggt
     1801 gctgggcctg cggcagggag gtgtcagtga agaaacacaa aatgacagct gtgagaaaat
     1861 actgacgccg acagagtgga aactactgtg ggttaagctg gaaggaaagc cgcttccggt
     1921 gcaggcaccg accctgaaat gggcctgggg cgatggcatg acagcaaacg cacaggtcgt
     1981 cccggttggg gcgtcatgtg ggatggctgg tcagacttca ggatatggtt gagggttacc
     2041 tggtgatgaa gtctcttgat caggagctct gatcaagaga cagcagctta ggggggtgcc
     2101 cctattaagt tgtcaagcat gttatgaccc ctgcggggta taaaaagtcc cgtcgcgcat
     2161 catggcgaac agaacgtcgc agcgtcgtct cgccagggcg ataagcgcct gattgtgtcg
     2221 ttttccctga ctcattttgc gggtgtagta agccctggag agcggatccc tgagcgcgg
//
LOCUS       DAZXUK010000400         2257 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZXUK010000400 DAZXUK010000000
VERSION     DAZXUK010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.403"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..956)
                     /locus_tag="ACH3L8_004155"
     CDS             complement(<1..956)
                     /locus_tag="ACH3L8_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFT4087008.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="ACH3L8_004156"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="ACH3L8_004156"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFT4087009.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2257)
                     /gene="yliI"
                     /locus_tag="ACH3L8_004157"
                     /pseudo
     CDS             complement(1825..2257)
                     /gene="yliI"
                     /locus_tag="ACH3L8_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
BASE COUNT          553 a          598 c          536 g          570 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccaca
//
LOCUS       DAZXUK010000401         2257 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZXUK010000401 DAZXUK010000000
VERSION     DAZXUK010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.404"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..82
                     /locus_tag="ACH3L8_004158"
     CDS             <1..82
                     /locus_tag="ACH3L8_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFT4087010.1"
                     /translation="IRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(171..683)
                     /locus_tag="ACH3L8_004159"
     CDS             complement(171..683)
                     /locus_tag="ACH3L8_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFT4087011.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            878..1642
                     /locus_tag="ACH3L8_004160"
     CDS             878..1642
                     /locus_tag="ACH3L8_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087012.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1593..>2257
                     /locus_tag="ACH3L8_004161"
     CDS             1593..>2257
                     /locus_tag="ACH3L8_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFT4087013.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          646 a          493 c          570 g          548 t
ORIGIN      
        1 tatccgggac ggaatcatca gcgaccagac agcattgaga atgcttcagg agtacatcag
       61 gatccaatgc cttggggggt agcggtaatt ttactcatta tccttcaaat cagattctgt
      121 tgtcagagga atgggggaag ctggattcgc aagagctttt tatgggcagt ttagctaaaa
      181 aaaggcaaca cgaagctaaa actaccaaca taaagtgctg accagctagc agctgaaatc
      241 agactcactg tatacacttt cactggatga agttgcaaca ttcctgccac caaaggagca
      301 atgtaacgca gcacggcgat aaagcgcgaa gtgaaaagta ttgacacaga gttattttgc
      361 aactgaaggc gaacccgttc tattgtattg gaacggctcg atattataca tgcaagttga
      421 gggatatgcc caattattat accaagatgg taattaacga tagtaccgca ccatgcgcca
      481 ctcatgaccg tgattccagc ttcccatggt gaaagagtcg tcttgctgac tgtaattaca
      541 gtggccatca taattgatgc tggtggcaat atcgccgaaa tgagtaaagt ggattttgtg
      601 aacgctatta taaaaagaag cccccatagg cgatatggat gaagtacaaa atagttcatc
      661 aaagcattta tccactccat caatgtcttt ccttctgtga atttttcttt gctatttaac
      721 cgtgatacga cttaatttag gcttaaacac cttacattta caaggtactc ctgtgggggg
      781 ccctgccacg gagcgtcgca ggagtgagtg atgaggaaga attaaagcaa cctattgtga
      841 caggcagcga taacagtaaa tcacaggaag cataatcatg gcaaaaccgg actgggaggc
      901 catcgaatcg gcataccggg ccggagtcct tagcctccgt gatataggcg agaaatacgg
      961 cgttacagaa ggggctatca ggaagagggc caaaaagctt gattgggcac gcagtggcgg
     1021 tacgcaggtt tgcaaaaatg gtacgcaaaa aaggaaagtg cgtaccagca gaaagcctgc
     1081 cattactggc cttacacaaa aaagtacgca actaaaaaca gaatctacac cggatacgaa
     1141 accgatacgc ggaatgcgta ccgatccccc gactaaccca ttccaacccg gtaaccagca
     1201 ggcattaaaa cacggtggtt atgcccgtcg ccttctgctc aaagatgagg tgatagagga
     1261 cgctaaagcg ttgacgctcg aggacgaatt atttcgcctt cgtgctaaca accttgtcgc
     1321 tgcagagaat attggtcggt ggttggtgtc gctggaagat gctaatgggg accaggaaag
     1381 gaagatgctg attgaaaata tcagcgccgc cgagaaagca atgatgcgca atacagttcg
     1441 tattgagtcc atcgttggca cgcttgcgac ggtaggaaaa atatttgctg atacagccta
     1501 ccgcaaggcc gccactgata aggtgtctct ggaggctgat cgtcttcgcc gtgatgcagg
     1561 tattgatgat ggcaatggag agcgtgacct caatgacttc tactctgaca tccaaaccga
     1621 cgctgaatcc ggctttacgt agtttctgga ctatgcgggc acgtaacaaa gtgctttatg
     1681 gtggtcggtc atcgtcaaaa tcatgggatg ccgctggcat tgccatattt ctgtcgaata
     1741 aatacaccct gcgtttttgt tgtgcccgtc agatccagaa taaaatcgaa gagtcggtgt
     1801 ataccctgct caaaattcag atagacaggt ttggtctgcg gcaccgtttc cgtattctga
     1861 acaacaaaat cattaaccgg gttactggct cggaatttgt tttttatgga ttatggcgca
     1921 acatcgaaga aattaagtca ctggagggga tcgatatgtt gtggctggaa gaagcccacg
     1981 cactgacgga ataccagtgg aaaattctgg agccaacgat ccgtaaagag ggttcggaat
     2041 gctggttcat attcaacccc ggacttgtta ctgatttcgt ctggcgcaac ttcgttgttg
     2101 atccgcccga aggcactctc atccgcaaaa ttaactatga cgaaaatccg tttctgtctg
     2161 acaccatgct taaggttatc gacgcggcgc gacgccgtga tccggatggt tttaaacatg
     2221 tgtatgaggg cgttccggag tctgatgatg atgcggc
//
LOCUS       DAZXUK010000402         2256 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZXUK010000402 DAZXUK010000000
VERSION     DAZXUK010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.405"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="ACH3L8_004162"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="ACH3L8_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFT4087014.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="ACH3L8_004163"
     CDS             578..1027
                     /locus_tag="ACH3L8_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFT4087015.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1261)
                     /gene="ydcY"
                     /locus_tag="ACH3L8_004164"
     CDS             complement(1028..1261)
                     /gene="ydcY"
                     /locus_tag="ACH3L8_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFT4087016.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="ACH3L8_004165"
     CDS             complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="ACH3L8_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFT4087017.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1715..1810
                     /gene="yncL"
                     /locus_tag="ACH3L8_004166"
     CDS             1715..1810
                     /gene="yncL"
                     /locus_tag="ACH3L8_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFT4087018.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          560 a          506 c          540 g          650 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagtcg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt
     1081 ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc
     1141 gatcttcccg acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg
     1201 caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca
     1261 tactttctcc ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt
     1321 ggtgccgtca agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa
     1381 ctcatcggcc atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg
     1441 tgagaaagaa accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc
     1501 atatgttgat agagaaacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata
     1561 tgcgccattt tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca
     1621 gcaacttgcc cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact
     1681 atacacatag gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt
     1741 actgataaat gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg
     1801 gtttatttga tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga
     1861 cagtacgcaa gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc
     1921 tttttgctgg catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa
     1981 acagcttact gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata
     2041 cgcgcgggag tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg
     2101 cgtaaagtat atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa
     2161 ttgcaaaccc cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc
     2221 tctgaatacc tcagaaagat tgaatttttt atagtc
//
LOCUS       DAZXUK010000403         2230 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZXUK010000403 DAZXUK010000000
VERSION     DAZXUK010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.406"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..222)
                     /locus_tag="ACH3L8_004167"
     CDS             complement(<1..222)
                     /locus_tag="ACH3L8_004167"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087019.1"
                     /translation="MFFSLSVLNLEDHLGLVISPQSPLSAGYITLRACRRSLPLRFST
                     VTPGGIVGFLIVSYRFNIPDRLAVHLSIHA"
     gene            651..2159
                     /gene="ltrA"
                     /locus_tag="ACH3L8_004168"
     CDS             651..2159
                     /gene="ltrA"
                     /locus_tag="ACH3L8_004168"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFT4087020.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          572 a          548 c          657 g          453 t
ORIGIN      
        1 ggcatggatg cttaaatgaa ccgcgagtct gtctggaata ttgaaccggt aactcacgat
       61 gagaaaccca acaatcccac cgggtgtgac ggtggagaac ctgagcggca gtgacctgcg
      121 gcatgcccgc agggtgatgt aacccgctga caacggggat tgaggcgaga tcactaagcc
      181 gagatgatcc tcaaggttaa gtactgaaag gctgaagaac atgaacccgt taatccgcct
      241 ctgtgggttg aaaacgtcac cacggcctac gtgatctgac aggccgtgca ggaggaactg
      301 gcagtgatac gtaagcactg ccggtcgaag gtgttttgac atgtatgcga aacaccgggg
      361 cagcagcgtc tatcacactt gcgttgctga cttctgccaa cttgcggcaa gcaaggataa
      421 agagtgcgac gggcagcctc ctcagtatgc ctgagtccag gcaggtaaac cggggaaggt
      481 cagcgacgga tgttaagggg gcatggctcc gatgacgcgc tggctggcgg agcttccgta
      541 gtagtccgcg atggggaaag cccattacat ggcgaaggga agcagtttga atgtgtttgc
      601 gacgtgaatt aactgaccta acgaggtgaa gacctttgat aatcagcgaa atgcaacgca
      661 agcttgccac atgggcagcc accgatccgt ccctacggat tcaacggctg ctgcgtctga
      721 taacacaacc agaatggctg gctgaagcgg cgcggatcac gctttcatca aagggggccc
      781 atacccccgg cgttgatggc gtgaacaaaa caatgctaca ggccagactg gctgttgagc
      841 tgcaaatcct cagggatgaa ttactctcag gccactacca gcccttgccc gccagacggg
      901 tttacatccc taaaagcaac ggcaaactgc gaccactggg tatccccgcg ttgcgggatc
      961 gtattgttca gcgggccatg ctgatggcga tggagccgat atgggagagt gattttcata
     1021 cgctctcata tggcttccgg cctgagcgca gtgtccacca cgcgatccgc acggtgaaat
     1081 tacagctcac agactgtggt gaaacccggg gacgctgggt gattgaaggc gacctgtcca
     1141 gttacttcga caccgtacat catcgactgc tgatgaaggc tgtacgccgc aggatcagtg
     1201 acgcacgttt catgactctg ctgtggaaaa ccatcaaggc gggacatatc gatgtcggtc
     1261 tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt tatatcgccg ctattatcga
     1321 acatcatgct gaatgagttc gatcaatacc tgcatgagcg ctacctgagc gggaaagcca
     1381 gaaaagatcg gtggtactgg aataacagta tccaacgggg ccgaagtacg gcggtcagag
     1441 aaaactggca gtggaaaccc gcggtggcgt actgccgcta tgccgatgat tttgtcctca
     1501 tcgtcaaagg caccaaagca caggcggaag ccatcaggga ggagtgtcgg ggtgtgctcg
     1561 aaggcagtct gaaactcagg ctgaacatgg ataagactaa aatcacccat gttaatgacg
     1621 gctttatctt tctggggcac aggatcattc gcaaacgcag tcgttatggc gagatgcgag
     1681 tggtctcaac gatcccgcag gagaaagcca gaaacttcgc cgcatcgctg acagcactgt
     1741 tatcaggcaa ctacagtgaa agcaaagtcg atatggctga acaactcaac cgaaaactga
     1801 aaggctgggc catgttctat cagttcgttg attttaaggc caaagtcttc agttatatcg
     1861 accgtgtcgt gttctggaag ctggctcact ggctggcccg caaataccgt acaggtatcg
     1921 cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg tcagagcaaa acgtgggttt
     1981 tatttggtaa aaccaatcac ggcaagctca gcggcgaaat actgtaccgg ttggtggggc
     2041 aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa tccctatctg aggacggaga
     2101 ccagaaacac gtatacatcg cgctttacag aagtggcaat ggcgttcgcc agcatttaaa
     2161 tggagagccg gatgcgctga aaggtgcacg tccggttcgg ggaggagagg cagggaaata
     2221 gtccgactac
//
LOCUS       DAZXUK010000404         2220 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZXUK010000404 DAZXUK010000000
VERSION     DAZXUK010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.407"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            6..224
                     /locus_tag="ACH3L8_004169"
     CDS             6..224
                     /locus_tag="ACH3L8_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFT4087021.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="ACH3L8_004170"
     CDS             226..591
                     /locus_tag="ACH3L8_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFT4087022.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="ACH3L8_004171"
     CDS             588..1253
                     /locus_tag="ACH3L8_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087023.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="ACH3L8_004172"
     CDS             1253..1618
                     /locus_tag="ACH3L8_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFT4087024.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2073..>2220
                     /gene="hokD"
                     /locus_tag="ACH3L8_004173"
     CDS             2073..>2220
                     /gene="hokD"
                     /locus_tag="ACH3L8_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFT4087025.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEP"
BASE COUNT          607 a          466 c          593 g          554 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     2101 ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct
     2161 gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt cttcacagct tacgaacctg
//
LOCUS       DAZXUK010000405         2219 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZXUK010000405 DAZXUK010000000
VERSION     DAZXUK010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.408"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            13..69
                     /locus_tag="ACH3L8_004174"
                     /pseudo
     CDS             13..69
                     /locus_tag="ACH3L8_004174"
                     /inference="COORDINATES: protein motif:HMM:NF027197.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            70..1224
                     /locus_tag="ACH3L8_004175"
     CDS             70..1224
                     /locus_tag="ACH3L8_004175"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFT4087026.1"
                     /translation="MYLALTARTRSLTLICLLSLYPLSTALVLTQSRVGLLGLFCVIV
                     LAALADRPRWRGRPLSGLLRLIFVASLLCWCVGISNYAMPGETTADFTHSSSNDQRIR
                     ILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGVTNIESDTATHPHNELLFVTAEGG
                     IVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEKWFIALMGLPLVIHMMTEYPLYTS
                     VPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILPVIVLIMTAALVILVAGFDAQREL
                     TRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHLLIANKPGFVRDTESMSLFTVWGN
                     KWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRAARVFVNDERFTRGGN"
     gene            1224..1958
                     /locus_tag="ACH3L8_004176"
     CDS             1224..1958
                     /locus_tag="ACH3L8_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFT4087027.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
BASE COUNT          559 a          455 c          563 g          642 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgcttg caaatatagg tgatttttt
//
LOCUS       DAZXUK010000406         2218 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZXUK010000406 DAZXUK010000000
VERSION     DAZXUK010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.409"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACH3L8_004177"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACH3L8_004177"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFT4087028.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACH3L8_004178"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACH3L8_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFT4087029.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACH3L8_004179"
     CDS             complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACH3L8_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFT4087030.1"
                     /translation="MQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          589 a          610 c          555 g          464 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac taggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgg
//
LOCUS       DAZXUK010000407         2202 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZXUK010000407 DAZXUK010000000
VERSION     DAZXUK010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.410"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..344
                     /gene="spa15"
                     /locus_tag="ACH3L8_004180"
                     /gene_synonym="spaK"
     CDS             <1..344
                     /gene="spa15"
                     /locus_tag="ACH3L8_004180"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFT4087031.1"
                     /translation="PSIITDLDSHSAITISLDSMPAINIALVNEQVMLWANFDAPSDV
                     KLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRVVIKDDYVHDGIVFAEILHEF
                     YQRMEILNGVL"
     gene            348..1640
                     /gene="spaL"
                     /locus_tag="ACH3L8_004181"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             348..1640
                     /gene="spaL"
                     /locus_tag="ACH3L8_004181"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFT4087032.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            1721..2071
                     /gene="spa13"
                     /locus_tag="ACH3L8_004182"
                     /gene_synonym="spaM"
     CDS             1721..2071
                     /gene="spa13"
                     /locus_tag="ACH3L8_004182"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFT4087033.1"
                     /translation="MYRDVEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVK
                     KHIIQYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLI
                     LDELSQEDMKYGIR"
     gene            2058..>2202
                     /locus_tag="ACH3L8_004183"
     CDS             2058..>2202
                     /locus_tag="ACH3L8_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087034.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIA"
BASE COUNT          712 a          325 c          475 g          690 t
ORIGIN      
        1 ctccatcaat tattacagat ttagacagcc attcagcgat taccatttct ttggatagta
       61 tgcctgctat taatatagct cttgtcaatg agcaggttat gttatgggct aattttgatg
      121 caccaagtga tgtaaaactt cagtcatctg cttataacat attaaattta atgttaatga
      181 actttagtta tagcattaat gagctggtag agcttcatcg ttctgatgag tacttacagt
      241 taagagttgt tattaaagat gactacgttc acgatgggat tgtttttgca gagattctgc
      301 atgagtttta tcagaggatg gaaatcttaa atggggtctt ataatcaatg agctatacaa
      361 aattgctcac tcaattatct tttcctaata gaatctcggg gccaatcttg gaaacaagtc
      421 ttagcgatgt ttcgattggt gagatttgta acattcaggc tggaattgaa agtaatgaaa
      481 ttgttgcaag agctcaggtt gtaggatttc atgatgaaaa aacaatatta agcttgattg
      541 gaaattctcg tggactttca cggcaaacgt tgattaagcc cactgcccag tttcttcata
      601 cgcaagtcgg ccgtggatta ttgggagcag tagtcaatcc tttaggggag gttactgata
      661 agtttgctgt tacagataac agtgaaattc tttatcgacc tgtagataat gctcctccgc
      721 tatatagtga aagggctgca attgagaagc cttttttaac aggtattaag gttattgatt
      781 ctttactcac gtgtggtgaa ggacagcgaa tggggatttt tgcgtcagct ggttgtggca
      841 aaacttttct catgaatatg ctcattgaac atagtggtgc tgatatatat gttattgggt
      901 taattggtga gcgaggtcga gaggttactg aaacggttga ttatttgaaa aactctgaga
      961 aaaaaagcag gtgtgtttta gtatatgcaa cttcggatta ctcttcggtt gatcgttgta
     1021 atgctgcata tatagccact gctatagccg aattttttag gactgaagga cataaagtag
     1081 cgctttttat tgattcatta acaaggtatg ccagagcatt acgtgatgtg gccttagccg
     1141 ctggagaatc acctgccaga agaggctatc cggtttcggt ttttgatagc ttacccagac
     1201 ttcttgaaag gccaggaaag ttaaaggcag gtggctctat tactgcattt tacactgttc
     1261 ttttggagga tgatgatttt gctgatccat tagctgaaga ggtaagatcc attttagatg
     1321 gacatatata tttgagcaga aatctagccc aaaaaggaca atttcctgca attgattcct
     1381 taaaaagtat aagcagggta tttacacagg ttgttgatga aaaacatcgt attatggccg
     1441 ctgcatttcg ggagctactt tctgaaatag aagagctaag gacaattatt gactttggtg
     1501 aatacaaacc gggggagaat gcctctcagg ataaaatcta caacaaaata tctgttgttg
     1561 agagttttct gaagcaagat tatcgactcg gttttactta tgagcagaca atggagctta
     1621 ttggtgaaac aattagataa ggttttaaaa atcaaagata aatatcaacg gtctgttaaa
     1681 ttaattgaag cacacatatt aacactaaaa aaaaaactct ttgtatcgag atgtggaggc
     1741 attagataaa aggattatat acttcctgca actggagaat gacttagaac ctgtaggcgc
     1801 tcagagtgtt agtcagcttt tcaatacaag acgtaagatc gctatagtaa aaaagcatat
     1861 tatccaatat cagtctgaac gaattcttct aaagggaaga attgaagaaa ttcaaaaaga
     1921 tattgatgag gcaaatgctt ctaaaagaaa actgcttcat aaagagagta aaatatgtaa
     1981 acggattggc ttgattaaaa gaaataactt tgccaaacag ttgatacttg atgagttatc
     2041 acaagaggat atgaagtatg gcattagata atataaacct aaattttagt agtgacaaac
     2101 agatagaaaa atgtgagaaa ctatcttcaa tagataatat tgatagtctc gttttgaaga
     2161 agaagaggaa ggttgaaatt cctgagtact ctttaattgc at
//
LOCUS       DAZXUK010000408         2201 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZXUK010000408 DAZXUK010000000
VERSION     DAZXUK010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.411"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..276)
                     /locus_tag="ACH3L8_004184"
     CDS             complement(<1..276)
                     /locus_tag="ACH3L8_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087035.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRS"
     gene            377..664
                     /locus_tag="ACH3L8_004185"
     CDS             377..664
                     /locus_tag="ACH3L8_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFT4087036.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            636..2165
                     /locus_tag="ACH3L8_004186"
     CDS             636..2165
                     /locus_tag="ACH3L8_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFT4087037.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
BASE COUNT          711 a          483 c          454 g          553 t
ORIGIN      
        1 gctgcgaagg aattcaggtt ttagtgtgga atgcactgga taagaattca tcatgtaata
       61 aaaatgttca tatcgggcat atatgatccg ccgttgctcc tgactgaaca ccccttcagc
      121 atcaataata cgactgatct cattataacg ttgatcccag aatttttgat gcatttcaaa
      181 attggtactc ttccgcttgt gtgccggatt ctcgccttcc ttaaacaacc ccaaaacagt
      241 acaaatatca tacaacatat ttgtaaatgt gcgcatatgc tgtgctctcc ggttggtttg
      301 cattttatcg cagaggacat tctgagctta acgtatcaaa ctgacacaac gacatgcaat
      361 gcatatggag aacaatatgt caaatcaaac cggacagctt tacacgcctt cacgcatcct
      421 gcgtatggct gaacttaccc gactgctggg tatatcaaga tctactattt acgtgaaaat
      481 aaatccggcc tcgaaatacc acgaccctac ctttcccaaa ccaataaggc ttggtagtgc
      541 atccgtaggc tggcgggcat ctgccatcga tgagtggctg atgttacata cggcaccagc
      601 ctggtctgaa cctgaaaaca aactaaagga acttaatgat gacgacaaaa ctatcactcc
      661 ctgaacttga cacaactaaa ctgcctgaca ggtgccaggc attacttgat gaaatgcatg
      721 aagaaacagg aatacgccgt gaaatacttc tgtctgttat gctgacggtc atggctgcct
      781 cggtccagga tacgcatgaa gttgaacttt ccggagggca acgtaccagc cttcagatat
      841 ttatgtgtct ttcatcagca tcaggcagtg gaaaaacctc tgcctgcgca aaattaatcg
      901 ctcctgttca tgaaacagaa gaagaactac atcaggcata tatcgacgat aaaaaaaact
      961 atgaccgaat gatggaaatg tggacaacag ataaaaaaat cctggagcgg agatataaaa
     1021 aggaaattga aagatccccg gaaaatgctg ctgctgcacg agcagcactt gaaaagtgta
     1081 tagcaaataa acccgaacct cccgtacaac aggttctcat cgtgaatgac gcgacaccag
     1141 aagggatagc tttgaaactc agccagtcac cctctcttct gttgctgtct gatgaaggag
     1201 gtactattct ggataagcgt ttcgaacgca aatcggcact gtacaacaca ttatggagtg
     1261 ggcaaccagt caacgttgaa cgggcatcca gacccgggtt ccggctgaat gatgtccgtc
     1321 tcactatact tatactgact caaccggtaa tatttaataa gttttttact ctcactggcg
     1381 atcagatccg gggcaatggc tttctggccc ggttgctatt ttgcgaaccc ggagcaatta
     1441 aaataatgac gacagaaccg catactgcca ctcctgttgt gacgcaacaa tgtgcaagct
     1501 gttttcgtaa gaaaagtttt ggcagtcaga tcagagactc tctgagagca tccagagaaa
     1561 gacgtgcaaa aagtaaagag cgcatctgca tgacattatc aaatgcagca tcccgcgcac
     1621 tggagcgctt tcacaaagaa aatatggata ctgtcagaca gaatcctcat atgggtacat
     1681 ttgaagacat tattgtcaga aaaagagagc aggttgtcag gatagcagca cttctggaac
     1741 tggagaaaga tccagactcg acagtaataa cgctggaaag tactaatagc gccatttatc
     1801 tcattgaatt ttatttcaag catcttatat ataaacttga atcccttcgg gaaatatccc
     1861 cggctgagaa acttgataaa tggcttcaga aaagaattat cacaacagca ggttacatat
     1921 tccagaaaag ttatatttta caatatgccc catatgctct caggaaaaaa tgtgttctgg
     1981 atgaagcact tgacatactg gcggaacaaa ggaaaataag gattgatgat aacctggttg
     2041 tttatatagg aaacacgatt acaccatccg aactggcaaa aaaactaaat attcctgcat
     2101 tcgatgccgg agttttcatt tgtgatcatc aaaacatact gaagtatcac cgaaacagac
     2161 tatagatact atcggacaaa aaataaatta tcacaagtgt a
//
LOCUS       DAZXUK010000409         2199 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZXUK010000409 DAZXUK010000000
VERSION     DAZXUK010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.412"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            440..1153
                     /locus_tag="ACH3L8_004187"
     CDS             440..1153
                     /locus_tag="ACH3L8_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087038.1"
                     /translation="MSQQVVIFNNEFSMSSYEFLIEVVNPARAEAGEAPVRNDQFLAR
                     VRDELDDCKTFATERITPRGGGRQMESITLTGDQLLLVGMRESKAVRRKVLDYIRRIE
                     KDKQLLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKNPISNE
                     WSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPTTVDKLRGILIAFTGTD
                     QQKQEHLLKQAQSGKLEGL"
     gene            1155..1349
                     /locus_tag="ACH3L8_004188"
     CDS             1155..1349
                     /locus_tag="ACH3L8_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087039.1"
                     /translation="MIDPRTPEGRMTLRYRGYRTEVILKELGLDPEDETRQHQSRDEL
                     IAQLVAMKLSSASKLAPAHR"
     gene            1470..1961
                     /locus_tag="ACH3L8_004189"
     CDS             1470..1961
                     /locus_tag="ACH3L8_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087040.1"
                     /translation="MLFNNQDWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRIEAERITKELASQKE
                     RTREMFMTEAEWQKELQRREQVKTERRTYGENLRSATHSAGRSKASIMADLDSGANWM
                     DSI"
     gene            2026..>2199
                     /locus_tag="ACH3L8_004190"
     CDS             2026..>2199
                     /locus_tag="ACH3L8_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087041.1"
                     /translation="MTLTKDALQFGIEPVRITDTDNLQINEGLPTNADPQVYAFQLAK
                     LVKTMLNSVLKDAQ"
BASE COUNT          566 a          584 c          535 g          514 t
ORIGIN      
        1 catacttcgg gtctaactgg aggtcagatt atggccttat caggtagcaa atcttatacc
       61 ctcactatag ggaatggacc cttagctatc actataggga ccaacattcc gcctctggtc
      121 ttgatgcgtc aagttgtgcg gaagctctcc cgacattaag tctttctcca taccaaggct
      181 agcaagacag cctctgtcag actcccagcc cgttagcgtt ctcgtgtctt cattttgact
      241 tgggtctatc gccgctcacc atctggacgt cggacatgct gtcttgcacc tttatcacta
      301 ctggtcatcg cagaccacat cacaattacc tcaagtcatt ctgtttgcgt cctgttgcct
      361 gctggtctcc ctttacgtgg gggcttgagt aggtcagtag ggtgcgactt cagggtgcaa
      421 acaaaaggag tacacgtaaa tgtctcaaca agtagtcatc tttaacaatg aattctctat
      481 gtcttcctat gaatttctaa tcgaggttgt caaccctgcc cgtgcagagg ctggcgaggc
      541 tccggtccgt aatgaccaat tcttagcccg tgtccgcgat gagctggacg attgcaaaac
      601 ttttgcaacc gaacggataa cacctagagg aggtggtcgc cagatggaat caatcacgct
      661 gaccggagac caattgttac tggtcggcat gcgcgagtct aaagctgtgc gtcgcaaagt
      721 ccttgactac atccgacgaa ttgagaagga caagcagtta ttggaagacc agaagaagcg
      781 agcagccatt cagtctgcta accgtcgagg cgtgacttgg ggggactact gcaaaaccta
      841 tggtctgccc gctcagaagc taatgaccgc acttcttcag catcgtggtc tctttcgtaa
      901 gaacccaatc agtaacgagt ggtctgtaaa tccaaagtat tccgactgct tccgtatcat
      961 taagccgtca gaccagaagt tctcagcagg tgggtataac ttccggttca acgcaaaggg
     1021 actggaggtc ttcggtaagc cgacgacagt cgataaactg cgaggcatcc ttattgcctt
     1081 cactggcacc gaccagcaga agcaagagca cctcctgaag caggcccaat ccggcaagct
     1141 ggagggactc taagatgatt gacccgcgca cacctgaggg ccgcatgact ctccgttacc
     1201 gtggctaccg gactgaagtc atcctgaagg agcttggtct agacccggaa gatgagaccc
     1261 gccagcacca atcacgagac gagctaatag cccaactggt cgctatgaaa ctgtcgtcgg
     1321 ctagcaagct agcgccagca catcgttagt agctcaccta cttaatacct aattttaaac
     1381 cttttgagat gaccgatgcg gggagacgcc gcaggactaa acacgcctgt agcagaccga
     1441 tagtctcaga ctcaagagga gtaattacta tgttattcaa taaccaagac tggaaactgt
     1501 cagtcaccga catcaatctc tacgagaata ctgtgagtct ggacggtcag tcctatccgt
     1561 tgtcgctcgc tatcaagacc ctgattccgg gctacttgtc cggcctgccg tctaccagcc
     1621 gcgaggcaat ggagttactg gaagctctgg ctgaagctgg cgtgaccatt ggtaacttct
     1681 tcagtaatga cctgatgact gcctaccaac gccgccagat gaataaacgt atcgaggctg
     1741 agcgtatcac taaagagctg gcgtctcaga aagagcgtac ccgcgaaatg ttcatgaccg
     1801 aggccgaatg gcagaaagag ctacagcgcc gcgaacaggt caaaactgaa cgccggacct
     1861 atggtgagaa cctccgaagc gctacccatt ctgctggtcg ttccaaggct tcaattatgg
     1921 ctgacctcga tagtggcgct aactggatgg attccatcta agaacacacg gtccctgact
     1981 taccgttagg gacttcaaat atctgactca aggagattaa ttatcatgac tctgactaaa
     2041 gacgctttac agtttggcat tgagcctgtt cgtatcaccg acaccgacaa ccttcagatt
     2101 aacgaaggtc tgccgaccaa cgcagacccg caggtctatg ccttccagtt agctaagctg
     2161 gtgaagacca tgctcaattc agtgcttaaa gacgctcag
//
LOCUS       DAZXUK010000410         2195 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZXUK010000410 DAZXUK010000000
VERSION     DAZXUK010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.413"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..331)
                     /locus_tag="ACH3L8_004191"
     CDS             complement(<1..331)
                     /locus_tag="ACH3L8_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087042.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            493..549
                     /gene="ysgD"
                     /locus_tag="ACH3L8_004192"
     CDS             493..549
                     /gene="ysgD"
                     /locus_tag="ACH3L8_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFT4087043.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="ACH3L8_004193"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="ACH3L8_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFT4087044.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="ACH3L8_004194"
     CDS             complement(1692..2072)
                     /locus_tag="ACH3L8_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFT4087045.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..>2195)
                     /gene="yigG"
                     /locus_tag="ACH3L8_004195"
     CDS             complement(2086..>2195)
                     /gene="yigG"
                     /locus_tag="ACH3L8_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HFT4087046.1"
                     /translation="AEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
BASE COUNT          569 a          523 c          513 g          590 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tagatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctggctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaaa taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggcta
//
LOCUS       DAZXUK010000411         2194 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZXUK010000411 DAZXUK010000000
VERSION     DAZXUK010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.414"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..427)
                     /locus_tag="ACH3L8_004196"
     CDS             complement(<1..427)
                     /locus_tag="ACH3L8_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFT4087047.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAGVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIF"
     gene            741..>2194
                     /gene="icsB"
                     /locus_tag="ACH3L8_004197"
     CDS             741..>2194
                     /gene="icsB"
                     /locus_tag="ACH3L8_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFT4087048.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKL"
BASE COUNT          774 a          425 c          323 g          672 t
ORIGIN      
        1 taaatatttg ttttattttc gtttgcaatt cctttttatt tttaatgcct agctgtgctc
       61 ttatctcttc acttattact ttattaagct cattaatatg tcttttatta acttcaccaa
      121 gtttataggt atctaatcgt tccatcatgg ctttaatttt tgacgtatca ccacctgcca
      181 actttattgc ctcgcaaaaa actccagctc tgtcacgcgc aaacaaaagg ctttttcctg
      241 ttaagcttgt ttggttctgt aaatataatc gattaagcgc ttcgatcccc ttatttcttt
      301 ctccattttt aactctttga tatgaaatca cgcttacacc tttgtgcttt ccggtttctt
      361 ctgcgccttt ataggaatct ccgctttgaa atgaaacctg atgcaatccc aaattagtta
      421 tgtgcattta attatcctca gtcataaatg gggcattgat tgcagttttg tacctcgttt
      481 tacaacaaaa aagatactta ttcaacaggg ttctctcttt ttctataaca ctccgagcct
      541 gatacatcag gcactttatc ttgtgggatt tcatgatgaa acgagcacta catatgctca
      601 cgaggtacat attatgtaca gcaaaagaaa atttgattat gttaacaggc tcaagtttct
      661 aaatttatta attgaatata ttatatacca aatacatttt acatccccac aatcaccaag
      721 taatggagag ttaataaagt atgatcctca aaattagcaa tttcattgac gcaagcaata
      781 caaaagggcc tatacgcgtt gaagatacag agcatggacc tatattgata gcacagaaat
      841 tcaacctaaa ggatctcttt ttcagaacat taagcactat taatgcaaag ataaattcac
      901 agatattaaa tgaacaacta aaaaattatc gactagagaa tcaaaaatcc ttattgcttt
      961 tcctcaatac attagctagc gaaaaatctg cagaaagtgc gtttgccgcc tatgaagcag
     1021 ctaaaaactc aattcaacac tctttcacag gtagagacat caaattaatg ttgaataccg
     1081 cagagcgttt tcatggcatc ggtacagcca aaaatcttga aagacattta gtttttcgtt
     1141 gctggggaaa tagaggcata acccatttag ggcatactag tatcagtata aaaaacaatt
     1201 tactacagga accaactcat acctatctca gctggtatcc cggcgggaat gttacaaaag
     1261 atacagaaat aaactacctt tttgaaaaac gctcaggtta cagtgtcgat acctataagc
     1321 aagacaaact aaatatgatt tcagaccaaa cagccgaaag acttgatgca ggccaagagg
     1381 ttagaaactt attaaattca aaacaagatc aaaataacaa caaaaaaata ttttttccca
     1441 gagctaatca aaaaaaagac ccctatggtt attggggggt cagtgctgat aaagtataca
     1501 ttccattatc aggtgataat aaaacaaagg atggtaaaat cagccataac ctttttggcc
     1561 ttgatgaaac aaacatgtca aaatttatat gcaaaaagaa agcagatgct ttcagacaat
     1621 tggcaaatta taaattaatc agtaaatctg aaaactgtgc tggtatggca cttaatgttc
     1681 ttaaagcagg aaattctgaa atatactttc cactccctga cgttaaattg gttgctacac
     1741 ctaacgatgt atacgcgtat gcaaataaag ttaggcaacg cattgaaagt ctcaatcagt
     1801 cttataacga aataatgaag tatattgaat ctgattttga tctttccaga ttgactcaat
     1861 tacgacgcag ctatcttaaa agtttcaata aaattaacct tatccacaca ccaaaaacat
     1921 tcaagccttt atcaatttca ctttacaaac atcctactga aaatgtatct tcagaagact
     1981 ttgatgcggt catcaacgct tgccactcat acttagtaaa atcagcaccc agcaatatga
     2041 ctcgtgtatt aaacgaatta aaaaccgaag caacagataa aaaagaagaa atcatcgaaa
     2101 aatcaataaa aattattgat tattacaatt ctcttaaatc gccagatctt ggaaccaaac
     2161 tttacattca cgacttatta cagatcaata agct
//
LOCUS       DAZXUK010000412         2186 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZXUK010000412 DAZXUK010000000
VERSION     DAZXUK010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.415"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(219..383)
                     /locus_tag="ACH3L8_004198"
     CDS             complement(219..383)
                     /locus_tag="ACH3L8_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087049.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1285..1569
                     /locus_tag="ACH3L8_004199"
     CDS             1285..1569
                     /locus_tag="ACH3L8_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFT4087050.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1557..2042
                     /locus_tag="ACH3L8_004200"
     CDS             1557..2042
                     /locus_tag="ACH3L8_004200"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFT4087051.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          539 t
ORIGIN      
        1 caaaatgatg gacattattt ttgtggaacc ggaggtaaca gaccagacgg tttaaatgag
       61 ccggttactt taaacgggtg atggacagca cctcaaaagt tcagatggat tatatttgtc
      121 tgcaataccc cgggtatttt cgatttgcga aatggatgga acgacttgct caggggatag
      181 ctgatggagt tactgaagta ccgaaggacc actaaaaatc acaggccggg aatttcggcg
      241 tttccggcgt aggtgtgcca cgaatgcatc cagtggcgtg atgttggatg caatgctgta
      301 tgagagatga ggggcggcgt ccagacgcag ccttcaaacc gccttgagct gctgccgtta
      361 agagcggtgg tggtgagcgt caaggttaaa aggcgcagaa catgtgtcag gtaggtgttg
      421 cggagatgaa cgaaagtgaa ctgtcgatga agtgtcgaaa ggaacctcta ggcgatgtcg
      481 aaaatgtgct atctacgcag cgcatgacga gtccagcaga tatctggtac tggctgggcg
      541 gcatccggca tataggcagc atgaacgcaa cacaggctct cgtacggaac gtgggaacct
      601 gtcgtctcga tgctaaggga gcggcgcgga gcagaggcac tgcaagcgcc tgagtaccga
      661 tgcgagacac aggggcagat ggcttcgtag tagtgaggaa gtgcctgtaa tgggcatgga
      721 gcgaaggggg tcagtcgttg gggctggttt atcggtcaac cggatagccg ggaggagccc
      781 atgagtcagc caaagccgtt cgaggtctct aagtatgcgg tttggaaagc ataccagcgg
      841 gtcaaagcta atcgaggtgc ggcaggtgtc gatgggcagt cagtcgaggc gtttgaggtt
      901 accaatcgaa gctatacccg gtaatggcgc aggttgacct gcacttggcc aagtggattg
      961 cccgcaaaca caagcgagcg cgaggtagtc tggtgcgtgc attcgagtgg ttactgcgaa
     1021 ttcgtcacga ctgcccgacg ctgtttgcac actggtgctt agcttacgat acttgaatgg
     1081 ttcaacgaca agagccgtat gacgcgagag tgtcacgtac ggctccgtgg gagcgtaggg
     1141 gggcggtgcc cctgcgccac ccgatatccc ctttgagtgc ggttacgtcc acagaggtaa
     1201 atgccgctga cgcggcatct gtaatctgtt actttgtgta tatcatttgc tatatattgt
     1261 caatatgcta ttgttgtaaa gcaaatgata tacggaggct ttatgaaatc aggtgttcag
     1321 ctcaatctca gagcaagaga atcacagcgg atactcattg atgctgctgc agaaattctt
     1381 cataagtcgc gtacagattt cattctggaa atggcctgta aagctgctga agatgttatt
     1441 ctcgaccggc gggtgtttaa ttttaatgac aggcaatatg aagagttcat tgaaatgctt
     1501 gatgctccgg ttgcagatga tcctgccatc gaaaaattgc tggcaaggaa acctcaatgg
     1561 gatgtgtaac tgcaccagaa cctttatcct catttcatca ggtcgctgaa tttgtcagca
     1621 gtgaagctgt acttgatgac tggttaaagc agaaggagct aaaaaatcag gcaatcggag
     1681 ccacccggac ttttgttgta tgcagaaaag gtacgcaaca aatagtcggt ttttactcac
     1741 tggccactgg tagtgtcaat catacagaag ctacaggtaa ccttcggcgc aatatgccag
     1801 accccattcc tgtaattata cttgcacgcc tggctgtaga tgtgtcattt cgaggaaaag
     1861 ggcttggcgc ggatttactt catgatgcag ttcgtcgttg ttacagggtt gccgaaaata
     1921 tcggtgtgcg tgcaattatg gttcatgcgc ttacggaaaa cgcaaaacag ttttatattc
     1981 atcatggttt taagccatca aaaactcagg tgcaaacatt gtttcttaaa ctccctcaat
     2041 agggagtaat gccgatcagt taaggatcag ttgaccgatc cagtggctgt gtaagaatcc
     2101 ggaaacgctc actcgtttcc ggatttttct gtgcacagta aaagaccacc tcctgaggag
     2161 gtggcttttc agaatgcccc ctataa
//
LOCUS       DAZXUK010000413         2183 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZXUK010000413 DAZXUK010000000
VERSION     DAZXUK010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.416"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..203
                     /locus_tag="ACH3L8_004201"
     CDS             <1..203
                     /locus_tag="ACH3L8_004201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087052.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSIH"
     gene            293..694
                     /locus_tag="ACH3L8_004202"
     CDS             293..694
                     /locus_tag="ACH3L8_004202"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087053.1"
                     /translation="MNGNTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDY
                     LPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSR
                     DGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            696..1301
                     /locus_tag="ACH3L8_004203"
     CDS             696..1301
                     /locus_tag="ACH3L8_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFT4087054.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="ACH3L8_004204"
     CDS             1288..>2183
                     /locus_tag="ACH3L8_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFT4087055.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DAZXUK010000414         2172 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZXUK010000414 DAZXUK010000000
VERSION     DAZXUK010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.417"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..357
                     /locus_tag="ACH3L8_004205"
     CDS             <1..357
                     /locus_tag="ACH3L8_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFT4087056.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            619..981
                     /locus_tag="ACH3L8_004206"
     CDS             619..981
                     /locus_tag="ACH3L8_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFT4087057.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            978..1907
                     /locus_tag="ACH3L8_004207"
     CDS             978..1907
                     /locus_tag="ACH3L8_004207"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFT4087058.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            1904..>2172
                     /locus_tag="ACH3L8_004208"
     CDS             1904..>2172
                     /locus_tag="ACH3L8_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase domain-containing protein"
                     /protein_id="HFT4087059.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIIC"
BASE COUNT          614 a          440 c          479 g          639 t
ORIGIN      
        1 gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa caaaaacagc agcggtaagg
       61 aagatgagaa ccgacagcaa caaggcatgg aagggagcgt taaagcgggc aggcattagc
      121 aacttccgct tccatgacct gaggcatacc tgggcaagct ggctggttca gtccggtgtc
      181 tctcttcttg cgcttaaaga gatgggagga tgggaaactc tcgaaatggt tcaaagatac
      241 gcccaccttt cagccgggca tcttaccgag cacgcgagca aaatcgatgc gattataagt
      301 cgcaatggca caaatacggc acaagaggag aacgtagttt acttaaatgc gaggtaactt
      361 attgatttaa atggtgccga taataggagt cgaacctacg accttcgcat tacgaattat
      421 aagaatccgc ttgtaattca aaggattatc ccatcaacac tgcgctcaca cgtcccacca
      481 catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct tatgtgtctc agttttgtct
      541 catcagacat agcaagtatc gatcaattga gacttggatg atagacttca tgcctttcag
      601 agctcattga ttaaataaat gttaaagtta tttgtaaagt acacatctat aggtgtgctg
      661 aacacactta tacactgggt ggtttttggt gtatgtatct atgccgcaca taccagtcag
      721 gctctggcaa acttcacagg tttcgtagtg gctgtgagct ttagcttctt cgcgaatgca
      781 agattcacat tcaaagcatt gactacagcg atgcgctaca tgttatacgt gggatttatg
      841 ggaatattga gtgtgattgt tggttgggca gctgataaat gctcacttcc tccaatagtc
      901 actcttatca ccttctccgc catcagtctg gtgtgcggtt tcgtctattc aaagttcatt
      961 gtctttaggg atgcgaaatg aaaatatctc ttgtcgttcc tgtcttcaat gaagaagaag
     1021 cgatacctgt tttctataaa acggtacgtg aattccaaga gttgaagcca tatgaagtag
     1081 aaattgtatt cataaatgac ggaagtaaag atgccacaga gtcaattatt aacgcgctgg
     1141 ctgtttcaga cccgctagtt gttccgctgt catttacacg caactttggt aaagaaccag
     1201 ccttatttgc agggttagac catgcaagcg gcgatgctgt aattcctatt gatgtcgacc
     1261 tgcaagaccc aattgaggtt atccctcatc ttattgaaaa gtggcaggca ggtgctgaca
     1321 tggtgcttgc taaacgttca gaccgctcaa ctgatggacg actgaaacgt aagacagctg
     1381 agtggttcta taaattacac aacaaaataa gcaccccaaa gatcgaggaa aatgtcggag
     1441 attttcgact catgtctcgt gaggttgtgg agaacattaa actgttgcct gagcgcaatc
     1501 ttttcatgaa aggcatactg agctgggtgg gtggtccgac ggatgtcgtt gaatatgtac
     1561 gcgcagagcg tgttgctggc atctcaaaat ttaatggctg gaaattatgg aatctggcac
     1621 tggaaggtat cacaagtttt tcaacctttc ctcttcgcgt atggacttat ataggcttgt
     1681 ttgttgcaag catttcattt ttatatggtg catggatgat tatagacacc cttgtctttg
     1741 gtaacccagt acgcgggtat ccctccctgc ttgtatcaat acttttcttg ggtggagtgc
     1801 aactgatcgg gattggtgtt ctcggagaat atataggtag aatctatatt gaagtaaaaa
     1861 atagacccaa atacatcata aaaaaatctc atcgaggtaa cccatgatta aaattaattt
     1921 atttaaaaat gcaaatctcc ttgccttcat atcatgtttt gcaatatcaa tttattgtta
     1981 ttgggggtgg ttgtacgatg gaacacttaa tattgatggc gagtttacaa ataattttta
     2041 tcaaacaatc acgcttgggc ggtggtttca cacttttttg cgacattact ttctccctga
     2101 gcctttttca ctttatataa caccattaat agccttatct tttatcatca tttcagcatt
     2161 tataatctgc ag
//
LOCUS       DAZXUK010000415         2160 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZXUK010000415 DAZXUK010000000
VERSION     DAZXUK010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.418"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>2160
                     /locus_tag="ACH3L8_004209"
     CDS             <1..>2160
                     /locus_tag="ACH3L8_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HFT4087060.1"
                     /translation="FSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNII
                     NACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAPD
                     RIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDYE
                     LAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEAI
                     LHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDL
                     CTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKIS
                     KDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKNL
                     HALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSIT
                     PPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDFS
                     LLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNHQ
                     QDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQL
                     YNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEG
                     TWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFT"
BASE COUNT          762 a          421 c          394 g          583 t
ORIGIN      
        1 tgttttctat aaaaccagga cctggaaatt tacctatcga caaccccaca ttgttatcat
       61 ggaacattac tgatggggat ctaaattcca aatttaatac attagaatat ctaaactgta
      121 taacaaatat tattaatgct tgtggagttt atcctcagga tttaaaagac agagaaatta
      181 tatcaacttt tcacgcagaa aaagtcatta atgatctgtt aaaaaacgat tataaaattt
      241 ccctttctcc agatacaact taccgagagc tgaataaagc tgcacagagt agcattacag
      301 cgccagacag gataggagaa gggaaaacat gggtttatca acgagataca atggttgaaa
      361 gaggtgataa cagtggtgtt catcagtatg gtccagctga acatttcact cacattatat
      421 ctgacaaacc gtccccaaaa gataagtatg ttgcatatgc tattaacatt cctgactatg
      481 agctagcagc cgatgtatat aatattaacg tgacgtcacc ttccggacag caagaaacat
      541 ttaaaatact aatcaatcca gaacatctac ggcaaacact tgagcgtaaa tctcttactg
      601 ctgttcagaa atcacaatgt gaaatcatca cccccaaaaa acctggcgaa gcgattcttc
      661 atgcttttaa tgccacctac cagcaaatca gggaaaatat gtctgagttt gcacgttccc
      721 attatgggta tatacaaatc cctccagtga caactttccg cgccgacgga ccagaaactc
      781 ccgaagaaga aaaaggttac tggtttcacg cttatcaacc cgaagatctt tgtaccatcc
      841 ataatccaat gggggatttg caggatttta tcgcattggt taaagatgct aaaaaatttg
      901 gtatcgatat cattcctgat tataccttta actttatggg aatcggggga agtggtaaaa
      961 atgacctgga ttatccctct gctgatatac gagcgaagat cagtaaagat atagaaagtg
     1021 gtatccctgg ctattggcaa ggtcaggttt tgattccatt cattaaagat ccagtaacaa
     1081 aagaacgtaa acaaatccat ccagaagata tacatctcac tgcaaaagac ttcgaagcaa
     1141 gtaaagataa catctctaag gatgaatgga aaaacctcca tgcattaaaa gaaaagagtt
     1201 taaatggaat gcctaaaaca acccccaaaa gtgaccaggt tattatgttg caaaatcaat
     1261 acgttcgtga aatgcgaaaa tatggcgtac gaggtttacg ttatgatgca gcaaaacact
     1321 caaaacatga acaaatagaa agatcaataa ccccaccgct taaaaattat aatgagcgat
     1381 tacacaatac taacttattt aacccaaaat atcatgaaaa aaccgttatg aattacatgg
     1441 aatatctggt aacttgtcag ttgaatgaag aacaaatgtc atcgctgctt tatgaaagag
     1501 atgatttaag cgccattgat ttttcattgc tcatgaagac gataaaagcc ttttcattcg
     1561 ggggagatct cctaaccctt gcatcaaaac cgggttccac aatctcaagc atcccatcag
     1621 aaagacggat attgattaac attaaccacg attttcctaa caatggcaat cttttcaatg
     1681 actttctatt taaccatcaa caagatgaac aattagcaat ggcatatatg gccgctctcc
     1741 cgttcagcag gcctttagtt tactgggatg gccaggtatt aaaatcaacg actgaaatta
     1801 aaaattatga tgggcccacg cgtgtcggtg gtgaggcgtg gcttaataaa ggttgctcta
     1861 cctatcagca gctctacaat gaattccacg cattatatat agataaagca ggaatatgga
     1921 gcgcatttga gggtgtattt gcaactaaaa acgttctggc ctttagtcgt ggggattctg
     1981 tgaacattaa tcactctcct catgatggac tagttataat aaataaagga aacgaagaag
     2041 ttgaaggtac ctggcctaac aaattgcaac ctggaatata taaaaacatg gggagtaata
     2101 gcgttaacat tattattaat aatacccgaa aaattatccc cccttgtaaa gtatttacgc
//
LOCUS       DAZXUK010000416         2144 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZXUK010000416 DAZXUK010000000
VERSION     DAZXUK010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.419"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..955
                     /gene="espL1"
                     /locus_tag="ACH3L8_004210"
                     /pseudo
     CDS             1..955
                     /gene="espL1"
                     /locus_tag="ACH3L8_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1127..1234
                     /gene="yniD"
                     /locus_tag="ACH3L8_004211"
     CDS             1127..1234
                     /gene="yniD"
                     /locus_tag="ACH3L8_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFT4087061.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1287..2045)
                     /locus_tag="ACH3L8_004212"
     CDS             complement(1287..2045)
                     /locus_tag="ACH3L8_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFT4087062.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          645 a          478 c          447 g          574 t
ORIGIN      
        1 gctcagacct gggctgatag gtatgttcgc aactgtgcaa ttgctattct gcccctatta
       61 cctgtgggag ttctaggggc tattttcgcg ggtattgcat cacgaatgcc atttgcccct
      121 atacatccat cggcaatgtt gttaataatg gctacaggcc agactcaaca gcttattaca
      181 ttattcaaac agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag
      241 aatagcgttg gtacacctgc tttatttttg gctatgatga acggacatac tgacaacgtg
      301 aaaatattta tgcaagaaat tcagtcactg gtagataatc acatcattca tgaagataat
      361 ctggttaaat tactgcaaac taaaagtgct aacgaaacac ctggacttta tatctccatg
      421 ttgtatggat tcgatgaaat aatcgatatc tttctgaata cattaaccac tccaatagca
      481 ctaagagctt ttaaacaaaa aactggtgat gagtatttta gccataaaaa tacatgatgg
      541 tgagccagga ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct
      601 ctctaaaatt aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt
      661 attaaaagga gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa
      721 tgaagacgtc gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt
      781 tagtcaacat cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt
      841 tcatatagcc attcatcata agcattataa aactgtagaa acatattatg ctgctataaa
      901 tgtcatcagc caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc
      961 tatttacgat atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac
     1021 catgtgcaca acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctgtccctt
     1081 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg
     1141 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
     1201 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
     1261 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
     1321 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc
     1381 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
     1441 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
     1501 catgcataag ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat
     1561 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
     1621 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
     1681 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
     1741 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
     1801 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
     1861 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
     1921 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1981 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     2041 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     2101 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataa
//
LOCUS       DAZXUK010000417         2126 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZXUK010000417 DAZXUK010000000
VERSION     DAZXUK010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.420"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..106)
                     /locus_tag="ACH3L8_004213"
     CDS             complement(<1..106)
                     /locus_tag="ACH3L8_004213"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFT4087063.1"
                     /translation="MCHPQPKPPAPPAWAMMPPSNSLQLLDETFSVSGT"
     gene            complement(66..401)
                     /locus_tag="ACH3L8_004214"
                     /pseudo
     CDS             complement(66..401)
                     /locus_tag="ACH3L8_004214"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            complement(434..931)
                     /locus_tag="ACH3L8_004215"
     CDS             complement(434..931)
                     /locus_tag="ACH3L8_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFT4087064.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRD"
     gene            complement(997..1626)
                     /locus_tag="ACH3L8_004216"
     CDS             complement(997..1626)
                     /locus_tag="ACH3L8_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087065.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1630..1845)
                     /locus_tag="ACH3L8_004217"
     CDS             complement(1630..1845)
                     /locus_tag="ACH3L8_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFT4087066.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          543 a          567 c          468 g          548 t
ORIGIN      
        1 ccgtcccgga taccgagaac gtttcgtcca gcaactggag agagttcgac ggtggcatca
       61 ttgcccatgc cggaggcgct ggaggtttcg gctgaggatg gcacagggca ttttcctttg
      121 acgagcaccc gaccaccatt atcaagcttg cgccgaagag catcattttc agctttcgca
      181 tcagctaact ccttcgtgta tttagcatcg agtgcatcag catcacgctg gcgctgctgc
      241 atgtcagtaa tggttgcgtt cgccagcttc agttctccgg cgtttttgtc gcgctgctct
      301 ttgtaggtaa tggcgttatc acggtaatga ttaacagccc atgacaggca gacgatgatg
      361 cagataacca gagcggagat aatcgcggtg actctgctca ttgatctatc ccccaacagg
      421 ctaatgcgct ttcctagtca cgacgaataa cctgtccata gcagttattt gaacgtatgc
      481 ggcaatcgcg cccaacatct tttatccacc agcgaatcgc ctcgcatgcg cccttacgat
      541 caccggcatt cagccgctta taaaacgtcg acgggaaaca cttaccgggg ccaatgttat
      601 aggggcaaaa tgacgctata cccgctttct ggggttcggt cagaggtact ttaatattgc
      661 gctccaccca tgccagcgcc ttatcacgtt caatagcgtt aacctggtca catttttcct
      721 tcgacagctt cattcccggt atgacaggct taccatccac cattgtggca ccacgacaga
      781 tggtccatat gccagaacca tcacggtatg ccgttgtgtg attaccctct ttttcgttca
      841 gaaactggtc aagtatctgg ggagcagacg cgcctgcggc aatcagcgcc agaacagcag
      901 ccgacaggcc gtatttgatt tttgcgctca tggatattta tcaggattta tcggcaacaa
      961 ataacgagcc aacttatata cgccttttaa gataagtcag ccccggctgg atccagtcat
     1021 caggctcttt cttaaagggg gagtattgaa atcacgaaga agagcctccc gcaccgcagc
     1081 atccatgtct gcaccactgg caagtgcttc aatctctgcc gccacctgca aataccccat
     1141 gcaacggcca atgcgtttca taactctggt gttttcgtct ttcaggcatc tgatagcgag
     1201 attaataatt ttcaggcagt gatgatgcga tgcccggaca ggttttccgt tctcataaac
     1261 gacgaactca ccctcttcac aggaaaccct gaaaggccga tggggttcct ggtctttatc
     1321 cggtgcagac gcaataaaat cacttttatg ctcttcctct ccggcactgt taaccgtaat
     1381 gacgtactca ggccgcaggt aagccgcctc tttttctgac aatgcggccg gtaaaaactt
     1441 ttcctgcaaa aacttcataa aagtctcgtg agcgactttg tggcagtaat cgtaaatcgc
     1501 cgcataatgc tcatcgcggc gtttgttttc atcttcagaa ctcaccaacg ccgacaattt
     1561 tttattaagc tcagtgattt cattttccag gtgattgaat cgctgattca tttcttcatg
     1621 cttcattatt tactctcccc gtgcagcctt acgcttgtct tctcttattt tgaaatacag
     1681 attcgtcaga taagtcagaa gccccagaag cagacttccc agcacaccaa tcgcagccca
     1741 ctgtgatggc ctgacctgat ccaaccactg caaaaaccag tagccggcac tgccggcgga
     1801 ggtgccgtag gcaatgcccg ttgaaatttt gtccatggat ttcatagcct cacctccgca
     1861 aataacggat ggcgtagttt tacactgaga aatgaaaggg atttgaaaag aaaaaaacgc
     1921 aaaagcgggc gaaacgatat atacagtaag gaaagcactc tatccaacaa accacccaca
     1981 gttaatcgga ataaaagcag agtgcttatg aatgatcgca cgcccgaagg ttagtatttc
     2041 tgcacagcaa ttttgcaaaa aaaggcgatc attcataact taaacgtctt tcagtcactc
     2101 cgggatttcc catcatcgca gactga
//
LOCUS       DAZXUK010000418         2124 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZXUK010000418 DAZXUK010000000
VERSION     DAZXUK010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.421"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(192..524)
                     /gene="pemK-mt"
                     /locus_tag="ACH3L8_004218"
     CDS             complement(192..524)
                     /gene="pemK-mt"
                     /locus_tag="ACH3L8_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HFT4087067.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            complement(526..783)
                     /gene="pemI"
                     /locus_tag="ACH3L8_004219"
     CDS             complement(526..783)
                     /gene="pemI"
                     /locus_tag="ACH3L8_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFT4087068.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(876..1529)
                     /locus_tag="ACH3L8_004220"
     CDS             complement(876..1529)
                     /locus_tag="ACH3L8_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFT4087069.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
BASE COUNT          556 a          582 c          508 g          478 t
ORIGIN      
        1 aacttcaaaa ctgcgcaaaa cacgcaaata cgcacaagaa agtagggctg cgcttggaat
       61 tcggaaaata aaccacatca aaactacttt ttagccaaat aacgtttggg aatcaccaga
      121 atggtgggac aacagcggtt ttagtgccct aaatcgtacg ttttcatcca gttgcccctc
      181 aaaccccatg ttcaagtcag aatagtggac aggcggccaa gaacttcgtt catgatagtc
      241 tccggaaccc gttcgagtcg ttttccgccc cgtgctttca tatcaattgt ccggggttga
      301 tcgcaacgta caacacctgt ggtacgtatg ccaacaccat ccaacgacac cgcaaagccg
      361 gcagtgcggg caaaattgcc tccgctggtt acgggcacaa caacaggcag gcgggtcacg
      421 cgattaaagg ccgccggtgt gacaatcagc accggccgcg ttccctgctg ctcatgacct
      481 gcggtaggat caagcgagac aagccagatt tcccctcttt ccatgtcaga tttcctcctg
      541 accagtcgcc ggtgcatcca gccattctcg ttcttcagct gatatttcag cattcggatc
      601 acactgtgcc agtagctcag ccagtgaata ttgcgggcgt ctgtacggct caacaatcag
      661 ccggccatta tcaatgacca tgccaacttc attatctgtg cccagagaca gcgcattcag
      721 cagtgccggt gggacggtca gcataactga gccgccaacc ctcttcagtc gggtggtatg
      781 cattcttcac ctccataaaa gttatattta aatataacat ccactaaaaa aacacaccag
      841 gctttaacgc acaatgttta ataaaaatat aactttcaac caaacagtaa acccagcgtg
      901 gttgcttcca tatgcagcaa taccggcagc agcaggccac cacttctgat cctggccact
      961 gatgtaatca accccaccag gaacagttct gccagtgtca gcaggttctg atactggctg
     1021 tgcgcggcga cgaacaacaa cgacgttatc agcgccccca gccacatcgt ccagcagtac
     1081 cgtgaacgga agacgttcag cataatcccc cggaacagcg tttcctcatt caacggggca
     1141 aggataaaga tggtcagcaa cgtcaggatc acgtcaggta tggacttatc ggcaaaaagt
     1201 ttcgtcataa atggctcagc aggcagagcc agcgccttac cgagcagaaa tacaccgaca
     1261 tacaccacgg ccatcgcacc gaccagccac ggtacgccaa cgttgcgcag ctgaccaacg
     1321 accggtagcg gagctatcca acggcggtat accaggaaaa cacacagcag gtacatcaga
     1381 acagtaccat gactgaagaa caaatagttt tttcctgatc cataaagcag aacggcctgc
     1441 tccatgacaa atctggctcc ccaactaatg ccccatgcag ccagcataac cagcataaac
     1501 tgcagatatt gattacgtgt ttgaatcatt gcatcgcctg taaattttta acttgtccta
     1561 tttttgtcat taccacgtat atacacatgt ataacaattc agatatcgtt accaggatat
     1621 gccgcatcag cggcatggaa ggcggcactc tgttgtttca tatgatacag gagtaaaacc
     1681 gccgaagccc ggcgtaagcc ggtactgatt gatagatttc accttaccca tccccagccc
     1741 tgccagacca tacccgcttt cagccatgag agagcttctg tgcgcggtcg gagtggtccc
     1801 gacgagggtt tacccgaagt cggggcgtat ctccgcgtta gcgggccgtg agggccgctt
     1861 acgagcgtgt actgagaact tccagcgaga agactgacag cgatgaagat gtagttacaa
     1921 cattcataat taaaagcgac tctgttccgg ccctttgggc cggggcgggg ccgcttttca
     1981 gttatgaggg aggggctttg tggtttcggt tctgcgctgg accggggttt ttctggaggt
     2041 tgtttttgtg tgttgtaact aaagtggctc cggtcggggc ccgccgcttg cggtgggagg
     2101 tgcatatctg tctgtccaca ggac
//
LOCUS       DAZXUK010000419         2094 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZXUK010000419 DAZXUK010000000
VERSION     DAZXUK010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.422"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            118..1155
                     /gene="curA"
                     /locus_tag="ACH3L8_004221"
     CDS             118..1155
                     /gene="curA"
                     /locus_tag="ACH3L8_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFT4087070.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1353..2018
                     /gene="mcbR"
                     /locus_tag="ACH3L8_004222"
     CDS             1353..2018
                     /gene="mcbR"
                     /locus_tag="ACH3L8_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFT4087071.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          445 c          560 g          512 t
ORIGIN      
        1 cggattcctc gatgaaatta ttgcgtgttg cagaggggat gtgacggctg caaacaaaac
       61 caatcccgtg agcctgctac tattgatgga ttcatgccaa aagcacagga gagcattatg
      121 gggcaacaaa agcagcgtaa tcgacgttgg gttctggcct cgcgtccaca tggcgcaccg
      181 gttccggaga atttccgtct tgaagaagat gatgtcgcca caccgggtga aggacaggtg
      241 ttactgcgca cagtttattt gtccctggac ccgtatatgc gtggacgtat gagcgatgag
      301 ccatcttatt caccgccggt tgatattggc ggcgtgatgg tcggcggtac ggtgagccgt
      361 gtcgtggagt cgaatcatcc tgattatcag cctggcgact gggtgctggg ctacagtgga
      421 tggcaggact atgacatatc cagtggtgat gacctggtga aacttggcga tcatccgcaa
      481 aatccatcgt ggtcgctggg tgtgctgggg atgccaggct ttaccgctta tatggggctg
      541 ctggatatcg gtcagcctaa agagggcgaa acgttggtgg tagctgcggc gacagaacca
      601 gtgggggcga cggtggggca aatcggcaaa cttaaaggtt gcagagtggt gggggtagcc
      661 ggtggcgcgg aaaaatgccg ccatgctacc gaggtgttag gcttcgatgt ttgccttgat
      721 caccacgcgg acgattttgc cgaacaactg gcgaaagcgt gcccaaaagg tattgatatc
      781 tattatgaaa acgtgggcgg taaggtattt gatgcagtgc taccattact taatacatct
      841 gcgcgcattc ccgtttgtgg cttagtgagc agctataacg ctacagagct accacccggt
      901 ccggatcgtt tacccctgtt gatggcaacg gtgctgaaaa aacgtattcg cttgcaaggg
      961 tttattatcg ctcaggatta tggtcaccgc atccatgagt ttcagaagga gatggggcaa
     1021 tgggtgaaag aggataaaat ccactaccgc gaagatatta ctgatggttt agagaatgcg
     1081 ccacagacgt ttatcggcct gctgaagggt aaaaacttcg gcaaagtggt gatccgcgtc
     1141 gcgggtgatg attaaagttt caacggcggc gtaagccgcc ataaaaatcc aggcaaaaag
     1201 cttattttca gctttaatta actaaccacg tcattgttct gctgctaata acaacaaagg
     1261 tgggtggttt atgattattc actttaatac actaggtgaa ctccttctgc catgcaggca
     1321 gggttggaca gaaaacacgc gaaggaatta aaatgcctgg aacgggaaaa atgaaacatg
     1381 tcagtttgac tctgcaggtt gagaacgacc tgaaacatca gcttagtatt ggcgcattaa
     1441 aacctggcgc acgcctgatt actaaaaatc tggcggagca attaggtatg agtattacac
     1501 ctgtgcgtga agcattatta cgtctggttt cggtgaatgc gctttctgtc gcacctgcac
     1561 aagcatttac agtaccggaa gtgaggcaaa gtcagttaga cgaaatcaat cggatccgct
     1621 acgaactgga attaatggca gttgccctgg ctgttgaaaa cctctcctcg caagacatag
     1681 cagaacttca ggaattgctc gggaaattac agcaggcgca agaaaagggc gacatggaac
     1741 aaatcattaa tgtgaacagg ctatttcgct tagcgattta tcatcgctca aatatgccca
     1801 tcctgtgtga gatgattgag caactgtgga tcaggatggg acctgcttta cattatcttt
     1861 atgaagcgat taacccagcg gaattacggg agcgcataga aaactatcat ctattactcg
     1921 ctgcgttaaa agcaaaagac aaagagggat gcaaacattg tcttgctgaa attatgcaac
     1981 aaaatattgc tattttatac cagcaataca atcgttaaat taaatgcccg gcaaataccg
     2041 ggcatcaagt aattactcaa atcgccacgc aatattcatg ccgacaccat aatt
//
LOCUS       DAZXUK010000420         2049 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZXUK010000420 DAZXUK010000000
VERSION     DAZXUK010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.423"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            125..1225
                     /gene="ompC"
                     /locus_tag="ACH3L8_004223"
     CDS             125..1225
                     /gene="ompC"
                     /locus_tag="ACH3L8_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFT4087072.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1389..1874
                     /locus_tag="ACH3L8_004224"
     CDS             1389..1874
                     /locus_tag="ACH3L8_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFT4087073.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
BASE COUNT          645 a          412 c          394 g          598 t
ORIGIN      
        1 attctatttc tctcattgtt tttaatcacc attctatttt aaaaacagta tgtcatttat
       61 aaatgacgcc gtttaaaatt cgtttagaaa atacgtttaa cgataattaa aaggattctg
      121 ttaaatgaaa ttaaaaatag ttgcggtggt tgtaactggt ttgttagctg cgaacgtagc
      181 acacgctgcc gaagtctata acaaggatgg taataaactc gacctttatg gcaaggttac
      241 cgctctacgt tattttactg atgataagcg tgacgatggt gataaaactt atgcccgtct
      301 cggctttaaa ggagaaacgc aaatcaatga tcaaatgatt ggttttggtc actgggaata
      361 tgattttaaa ggctataacg atgaagccaa cggctcgcgc ggcaacaaga cccgtctggc
      421 ctatgctggt ttaaaaatta gtgaatttgg ctctctggac tatggtcgta actacggtgt
      481 cggctacgac attggttcat ggaccgatat gttgccagaa tttggtggcg atacctggag
      541 tcagaaagat gtcttcatga cataccgtac caccggtcta gcaacctatc gcaactacga
      601 tttctttggc ttaattgaag ggctgaactt tgccgcgcaa tatcaaggca aaaataaacg
      661 tactgacaac agtcatcttt atggtgctga cgacacgcgt gccaacggtg acggtttcgg
      721 tatctcctca acttatgttt atgatggctt tggtatcggt gcggtatata ccaaatccga
      781 tcggacaaat gcgcaggaaa gagccgcagc taatcctctc aatgcctccg gtaagaatgc
      841 agaactatgg gccacaggta taaagtatga tgccaacaac atctactttg cagctaatta
      901 cgatgaaaca ttaaacatga ccacctatgg ggatggttat atctctaaca aagcacaaag
      961 ttttgaagtg gtggcgcaat atcaattcga cttcggcttg cgcccatcac tcgcttacct
     1021 gaaatcgaaa ggcagagatc tgggccgcta cgccgaccag gacatgattg agtatatcga
     1081 cgttggtgcg acgtatttct tcaacaaaaa tatgtcgacc tatgttgatt ataaaatcaa
     1141 cctgattgat gaaagcgact ttacccgtgc cgtagatatt cgcaccgata acatcgtcgc
     1201 tacgggcatt acctatcagt tctaacgtcc tctcctgacc agcccttctc tgggctggtt
     1261 tttgatttaa gtgctgctgt atgactcctt aagtaaaaat gagcaattct tgatatttct
     1321 ggtcattcac ctcaattaat attattccat atcctccggg gacatcttac tataatattt
     1381 attgatgagt gattatgacc aatattaata cagcttgtgt aaaaaataat gccagttatc
     1441 aacttaataa cgcattacct aacaaagaaa ccatctccag caatttttgt gaacgactag
     1501 aacaatgggg taataaatcg gttaataatg gtgaagaaag agcaattgcc gtggagcgaa
     1561 ttaaagaagc ttacaattcg aacatggcat ctcttgattt gtcatatctt gatttgagtg
     1621 aattaccgcc tatcccctcg acagttaata cgttaaatct ggaaaacaat tgtctaactt
     1681 gtcttgactt agctgacaat gccagtctcg ttaatatcaa cctcagcgtt aataaaatta
     1741 acacgataac ctttccaaaa gaaccaaaac tggaaaacat ttatattaat cataacaatc
     1801 tggaaagttt agatttaaaa aatcagcatt cattggttaa tctggaagcg caaaataata
     1861 gcctaaaaaa atgatatttc tgatagttat aaactgaaat ttcttaatct tgattataac
     1921 aagctagcat cgctggatgt ctcccggcaa gaatctctga ttgagttaag tgcccatcac
     1981 aatatgatca ctgaccttat attacacaat catcccagga tgaaaacaat cactttaaac
     2041 gacaaccat
//
LOCUS       DAZXUK010000421         2038 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZXUK010000421 DAZXUK010000000
VERSION     DAZXUK010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.424"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..423
                     /gene="hycA"
                     /locus_tag="ACH3L8_004225"
     CDS             <1..423
                     /gene="hycA"
                     /locus_tag="ACH3L8_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFT4087074.1"
                     /translation="AQRHRRLQDQWHIYCNSLVQGITLSKARLHHAMSCAPDKELCFV
                     LFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGMIDGKVNIRDREQV
                     LEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            548..1159
                     /gene="hycB"
                     /locus_tag="ACH3L8_004226"
     CDS             548..1159
                     /gene="hycB"
                     /locus_tag="ACH3L8_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFT4087075.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            1156..2038
                     /locus_tag="ACH3L8_004227"
                     /pseudo
     CDS             1156..2038
                     /locus_tag="ACH3L8_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
BASE COUNT          412 a          549 c          588 g          489 t
ORIGIN      
        1 gcacagcggc atcgtcgcct acaggaccag tggcacatct actgcaattc gctggttcag
       61 gggatcacgt tatcgaaagc gcgcctgcat cacgccatga gctgcgcgcc ggacaaagaa
      121 ctctgtttcg tcctttttga acattttcgt atttacgtca ccctggcgga tggctttaac
      181 agccacacca tcgagtatta cgtcgaaaca aaagatggcg aagacaaaca gcggattgcg
      241 caggcgcaac tgagcattga cggcatgatt gatggcaagg tcaacatccg cgatcgcgaa
      301 caggttctgg aacactatct cgaaaaaatc gctggcgttt acgacagctt atacaccgcc
      361 attgaaaaca atgtgccggt gaatttaagc caactggtaa agggacaaag cccggcagca
      421 tgagctgagg ctttgcccgt tttgcaggcg ttacgcctgt ttggggatgg gcgtgtcgat
      481 gagtgtcgaa aatgacattt catcggcatg ttttcgtcaa aaatgacaat cacctgagga
      541 atgcctggtg aatcgttttg taattgccga ctccacgctc tgtatcggct gccacacctg
      601 tgaggccgcc tgttcagaga cgcatcgcca gcacggcctg caatcaatgc cgcgcctgag
      661 agtgatgctc aatgaaaaag aatctgcgcc gcagctctgt caccactgtg aagatgcacc
      721 ctgcgcggtg gtctgcccgg ttaacgccat cacccgcgtt gatggggccg tgcagttgaa
      781 tgaaagtctg tgcgtaagct gcaaactgtg cggcatcgcc tgcccgtttg gcgcaattga
      841 attttccggc agccgtccgc tggatattcc ggcaaacgcc aataccccga aagcgccacc
      901 ggcaccgcct gctccggcgc gtgtcagcac attgcttgac tgggtgccag gtattcgcgc
      961 gatcgccgtc aaatgtgacc tttgtagctt tgatgaacaa ggtccggcct gtgtgcggat
     1021 gtgcccgact aaagccctgc atctggtgga taacaccgat atcgcccgcg tcagcaaacg
     1081 taagcgtgag ctgaccttta acacggactt tggcgatctc accttgtttc agcaggctca
     1141 aagtggagag gctaaatgag cgcaatttcc ctgatcaata gcggcgtggc atggtttgtc
     1201 gccgccgctg ttctggcatt tctcttttca aaaagcgcta agtggctgga tagccggaat
     1261 tggcggcgcg gttggtagtc tgtatacggc agccgcgggc ttcactgtac tgactggcgc
     1321 ggttggcgtg agcggtgcgc tgtcgctggt aagctacgat gtgcaaatct ccccgcttaa
     1381 cgcgatttgg ctgattaccc tcggcctttg cggtctgttt gtcagcctct ataacattga
     1441 ctggcatcgc cacgcgcagg tgaagtgcaa cggcttgcag atcaatatgt tgatggctgc
     1501 cgccgtctgc gccgtcatcg ccagcaacct cggcatgttc gtggtaatgg ccgaagtcat
     1561 ggccttgtgt gcggtgttcc tcaccagcaa cagcaaagag ggcaaactgt ggtttgcgct
     1621 gaggcgtctt ggcactctgc tgctggtgat tgcttgctgg ctgctgtggc agcgttatgg
     1681 cacgctggat ctgcgcctgc tggatatgcg tatgcaacag ctgccgctcg gttccgatat
     1741 ctggctgctc ggtgtgattg gctttggcct gctggctggg attattccgc tgcacggctg
     1801 ggtgccgcag gcacatgcta acgcctctgc gccagctgcc gcgttgtttt ctacggtagt
     1861 catgaaaatt ggcctgctgg gcatgttaac cctgtcactg ctgggcggta atgcaccgct
     1921 gtggtggggg atcgcgctgc tggtgctcgg catgatcacc gcgttcgtcg gtggtctgta
     1981 tgcgttgatg gaacacaaca tccagcgcct gctggcttac cacaccctgg aaaatatc
//
LOCUS       DAZXUK010000422         2032 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZXUK010000422 DAZXUK010000000
VERSION     DAZXUK010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.425"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..2032
                     /gene="sat"
                     /locus_tag="ACH3L8_004228"
                     /pseudo
     CDS             1..2032
                     /gene="sat"
                     /locus_tag="ACH3L8_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          746 a          357 c          399 g          530 t
ORIGIN      
        1 catacggcat gatgatttat aaaaatgata aaacatttcg taatcttgaa atattcggag
       61 acagcggctc tggagcatac ttatatgata acaaactaga aaaatgggta ttagtcggaa
      121 caacccatgg tattgccagc gttaatggtg accaactgac atggataaca aaatacaatg
      181 ataaactggt tagtgagtta aaagatacct atagtcataa aataaatctg aatggcaata
      241 atgtaaccat taaaaacaca gatataacat tacaccaaaa caatgcagat accactggta
      301 ctcaagaaaa aataactaaa gacaaagata ttgtgttcac aaatggggga aatgttctgt
      361 ttaaggataa tttggatttt ggttgcggtg gtattatctt tgacgaaggc catgaatata
      421 acataaacgg tcagagattt acatttaaag gagcaggaat tgatatcgga aaagaaagca
      481 ttgtaaactg gaatgcattg tattccagtg atgatgtttt acacaaaata ggccccggta
      541 ctctgaatgt tcaaaaaaaa cagggggcaa atataaagat aggtgaagga aatgttattc
      601 ttaatgaaga aggaacattt aacaatatat accttgcaag cggaaatggt aaggtaatac
      661 taaataaaga taattccctt ggcaatgatc aatatgcggg gatatttttt actaaacgtg
      721 gtggcacgct agatttaaat ggacacaatc agacttttac tagaattgcc gccactgacg
      781 atggaacaac aataactaac tcagatacaa agaaagaagc cgttctggca atcaataacg
      841 aagactccta catatatcat gggaacataa atggcaatat aaaactaacg cacaatatta
      901 attctcagga taagaaaact aatgcaaaat taattctgga tggtagtgtc aacacaaaaa
      961 atgatgttga agtcagtaat gccagtctta ccatgtaagg ccatgcaaca gagcatgcaa
     1021 tattcagaag cacagcgagt cattgctccc tggtatttct ttgtggaacg gactgggtca
     1081 ccgttttgaa agaaacagag agttcatata ataaaaagtt caattctgat tacaaaagta
     1141 ataatcagca gacctcattt gatcagcctg actggaaaac cggggtgttt aaatttgata
     1201 cattacacct gaacaatgct gacttttcaa tatcacgcaa tgccaatgtt gaaggaaata
     1261 tatcagcaaa taaatcagct atcacaatcg gcgataaaaa tgcttacatt gataatcttg
     1321 cagggaaaaa tattactaat aatggttttg acttcaaaca aactatcagt actaatctat
     1381 ccataggaga aactaaattt acaggtggca tcactgcaca taacagccaa atagccatag
     1441 gtgatcaagc tgtagttaca cttaatggtg caacctttct gaataatact cctataagta
     1501 tagataaagg agcaaaagtt atagcacaaa attccatgtt cacaacaaaa ggtattgata
     1561 tctccggtga actgactatg atgggaatcc ctgaacagaa tagtaaaact gtaacgccgg
     1621 gtctccacta cgctgctgat ggattcaggc tgagtggtgg aaatgcaaat ttcattgcca
     1681 gaaatatggc atctgtcacc ggaaatattt atgctgatga tgcagcaacc attactctgg
     1741 gacagcctga aactgaaaca ccgactatat cgtctgctta tcaggcatgg gcagagactc
     1801 ttttgtatgg ctttgatacc gcctatcgag gcgcaataac agcccccaaa gctacagtta
     1861 gcatgaataa tgcgatctgg catctaaata gccagtcatc aattaatcgt ctagaaacaa
     1921 aagacagtat ggtgcgtttt actggtgaca atgggaagtt tacaaccctt acagtggaca
     1981 accttactat agatgacagt gcatttgtgc tgcgtgcaaa tctggcccaa gc
//
LOCUS       DAZXUK010000423         2029 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZXUK010000423 DAZXUK010000000
VERSION     DAZXUK010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.426"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1824)
                     /locus_tag="ACH3L8_004229"
     CDS             complement(<1..1824)
                     /locus_tag="ACH3L8_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFT4087076.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2029)
                     /locus_tag="ACH3L8_004230"
     CDS             complement(1932..>2029)
                     /locus_tag="ACH3L8_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4087077.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          579 c          430 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagc
//
LOCUS       DAZXUK010000424         2028 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZXUK010000424 DAZXUK010000000
VERSION     DAZXUK010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.427"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..629
                     /locus_tag="ACH3L8_004231"
     CDS             <1..629
                     /locus_tag="ACH3L8_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFT4087078.1"
                     /translation="WSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLE
                     SALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRD
                     EFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQS
                     QKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(730..>2028)
                     /locus_tag="ACH3L8_004232"
     CDS             complement(730..>2028)
                     /locus_tag="ACH3L8_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFT4087079.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT          690 a          442 c          466 g          430 t
ORIGIN      
        1 gctggtcggt gaacttttac tcttttcagg cggcagccag cgatcgtggg cgggtggtcg
       61 acgatatcaa aacgaataat aaatatctga tcgtgaatag cgaagatttc aattatcgct
      121 ttagtcagct tgaatctgcg ttgaataccc agaagaactc gattcctgcg ctggaaaagg
      181 aagtgaaagc gcttgataag caaatggtgg cggcgcagaa agcagcggat gcatattggg
      241 gaaaagatgc aaacggtaag caaatgaccc gcgaagaagc ctttaaaaaa attcaccaac
      301 agcgtgatga gtttaataaa cagaacgata gcgaggcgtt cgcggttaaa tatgacaaag
      361 aggtttatca accggcgatt gcggcatgtc ataaacagag tgaagagtgt tatgaagtgc
      421 cgattcagca gaaacgagat ttcgatatca acgaacaacg gcgacagact tttctgcaat
      481 cacaaaaact cagccgtaaa ttgcaggatg actgggtaac gctcgaaaaa gggcaatatc
      541 cgttaacgat gaaagtttca gaaataaaca gtaaaaaagt cgcgattttg atgaaaattg
      601 acgatatcaa ccaagctaat gaacgctaga aaaaagatac cgaacaactg cggcgcaatg
      661 gggtgattaa gtaactcatg ttaagtgcct ttgccggata atataaaaag ttatccggca
      721 aattattttt tacattagcc ctagtagctg tggaatgaac aaggatattt gcggaatgta
      781 agtgatcatc atcaacgcta ctagcaatgc agcataaaat agcagcaatg gtttgattaa
      841 atgttgaatc ttcacacccg agatagaaca accaacaaat agcgcactac ccaccggcgg
      901 tgttaacagc ccaatacaaa ggttggccac catcataata ccgaaatgta ctgggtccat
      961 tcccagctct tgtgcaatcg gcaagaagat cggtgtaaag attagtacag ccggtgtcat
     1021 gtccataaag atgccgacaa ttaataagac gatattaatc agcagcaata tgatcagtgg
     1081 gttatcagaa atccccatca atgcgtcgct gatcatataa ggaatatcag cattagtcat
     1141 tgcccaggac atgcccacgg aaaaaccgat caacagtaat acaatcgaag tggtaacaac
     1201 cgactccaga atcagtttgg gtaaatggcg ccattttact tcccggtaaa tcagcaccga
     1261 gagaataaat gtgtagacaa cggcaattgc tgacgcttca gttgcggtaa aaatgccgcc
     1321 cagaatgccg cccatcacaa tgaacaccag aagcaaactt ggtaaggcat ccagaaacgc
     1381 tttacaggcc tgagcaaagg ttgctctttc tgaaagcggg tagccacggc gtttagcaat
     1441 aatgccgcag actatcatta ccgcaagacc cattaagatc cctggcaaat atcccgccat
     1501 aaacagcgat gctacagaaa cgccgccagc tgtcagggaa aacacaatta acacgtttga
     1561 gggcggtatc agtaatccag taatacaaga tgagacgttt acagcggtag aaaatgccgg
     1621 atcataacct tctttggtct gaatgggatt cagtgtgcca ccaacggcag cagctgccgc
     1681 aacggctgaa ccagaaatgg aaccaaacat catattcgcc aggacgttca catgaccaag
     1741 cgaaccagga acgcggccaa ccattacctg ggcgaggtta atcaaacgta tcgcaatacc
     1801 gccgctgttc atcagtgttc cggcaaagat gaaaaagggg attgccaaca gggcaaagtt
     1861 atccagtcca ttagccagtc gttgcgaaac agtgattgcc gcgatatccc agggaaacat
     1921 caaaagcatt gaagctacag tggcaatacc gattgagaac gaaatgggca caccaataaa
     1981 gaccagcaca aaaaaacagc caaacagtgt aatcgcgata taactatc
//
LOCUS       DAZXUK010000425         2017 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZXUK010000425 DAZXUK010000000
VERSION     DAZXUK010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.428"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..545)
                     /gene="ygeQ"
                     /locus_tag="ACH3L8_004233"
     CDS             complement(<1..545)
                     /gene="ygeQ"
                     /locus_tag="ACH3L8_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
                     /protein_id="HFT4087080.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1207..>2017)
                     /locus_tag="ACH3L8_004234"
     CDS             complement(1207..>2017)
                     /locus_tag="ACH3L8_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087081.1"
                     /translation="NIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVTA
                     PSGTEITVEPIEQAILVSHQTKLWDQKSSTGFTQGTLRQDALNICDNVVRTINSKMVD
                     VLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVTDYGIIAHESHLKAL
                     SRLAAKQGFGGEDAIVDMLGTDIAYYNGVDRGVFMMAKRFTALSFGCFRHDGENITVV
                     LSRDGNTQSHDLEILGKVFVVAEAATTIKMGTGSAAAVLPVVKRLSFTKTS"
BASE COUNT          551 a          456 c          482 g          528 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg agttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggagccgagg acaactagct aacttcttgt ttcttaaaga ctgatgaatc cagtggttac
      841 agtgatggtt acacatcgag aggctgacgg tcaagacttg aagcacgtaa tattcccata
      901 aaaggaatag aacacagtca ctgactaccc ggccctttgg tctgactgag ggtcgggcta
      961 tggggtaaat cgtcaggctt attgtattag atggtctctc acatttttca gtcaaaaact
     1021 catcgtactt taggccagac catcagcacg actaccagag ctagctttag caggaagacc
     1081 acgagataga ctttgtgtag gtccattagg tcctcctctt tccgtaccca tgaactagat
     1141 tgctagtaca aaataaaggc cagacatcca gttacggaag tctgaccaat aagtcgttat
     1201 cgttagttag cttgtcttag taaagctcag gcgtttaact acaggcagga cagcagcagc
     1261 gctaccagtc cccatcttga ttgtcgttgc agcctcagcg accacgaaga ccttaccgag
     1321 aatctcaagg tcgtgagact gggtgttgcc atctcgggac aggaccacag taatgttctc
     1381 gccatcgtga cggaagcaac cgaagctcag agccgtgaag cgcttagcca tcatgaatac
     1441 accacggtct acaccgttgt agtaggcgat gtctgtcccc agcatatcga cgatagcgtc
     1501 ttcaccaccg aagccctgct tagcagcaag acgggagaga gccttgaggt gtgactcatg
     1561 ggcgatgata ccgtaatcag taacctcaga accgaaagaa gactcggtgt tctcgtacag
     1621 ggcatccatg atagcgtctg ctttggccgt caggctcccg gtcaggacag gaagctcgac
     1681 ggtcttcagc agcttagagg actccagaac gtcaaccatt ttagagttaa tggtacggac
     1741 cacgttatcg cagatgttca gagcgtcctg ccggagcgtc ccctgagtga aaccagtaga
     1801 ggacttctgg tcccataact tggtctggtg ggacacgagg atagcttgct caatcggctc
     1861 gacggtgatt tcagtaccag acggagcggt cacgacttca ggtcgcttac caccacggac
     1921 aggaacgacc acagagcggt cagccagagt gtgagcaatt attggcgtcg gtaagctgtt
     1981 acgggtcggc aggacttcta cagggaacgg aatgttt
//
LOCUS       DAZXUK010000426         2006 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZXUK010000426 DAZXUK010000000
VERSION     DAZXUK010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.429"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(17..286)
                     /locus_tag="ACH3L8_004235"
     CDS             complement(17..286)
                     /locus_tag="ACH3L8_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFT4087082.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            343..1011
                     /locus_tag="ACH3L8_004236"
     CDS             343..1011
                     /locus_tag="ACH3L8_004236"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFT4087083.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1327..>2006
                     /locus_tag="ACH3L8_004237"
     CDS             1327..>2006
                     /locus_tag="ACH3L8_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFT4087084.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          607 a          432 c          372 g          595 t
ORIGIN      
        1 ggtttttctt tttcgcttac attgtgtctg gttcttccgg ccactcaata tcaggtgcag
       61 ttgatgtatc aacacggttc agcaacaccc gatacttctt ccaggcttcc agcaacgagt
      121 tttcttcctc cgttgcgatc tccagatcta cagcatcctg aagtggcgca atatgctcac
      181 tgaatccctg gatgtagaac tgtgtggtga cggtcttcca gccattcggc tcctgctgta
      241 tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg cagcatttaa ccccttgtaa
      301 ttcatcgcca taattgattt aattcacaaa taaaactata atatggtgaa attaatgaaa
      361 aaaaacacag atgatggggc taaaatttac acaccactta ccctaaagct ttatgactgg
      421 tgggttttgg gagtatcaaa tcggcttgca tggggatgtc ctacaaagga acaccttctt
      481 ccacactttc tggaacattt aggtaacaac catctggata ttggtgttgg aactgggttt
      541 taccttactc acgtacctga gagtagtctg atatctttaa tggatttgaa cgaagctagc
      601 ctgaacgcgg catctacaag ggctggggaa tcaaaaatta aacataaaat tagccatgat
      661 gtttttgaac cttatcctgc ggcgttacat ggtcaatttg attccatttc catgttttac
      721 cttcttcact gcctgcctgg aaatatatct acaaaaagct gtgtaatacg caatgcggcg
      781 caggccttaa ctgatgatgg aactctatac ggagccacaa ttcttggcga tggagttgtg
      841 cacaatagct tcggtcaaaa actgatgcgc atttacaatc agaaaggcat cttttcaaac
      901 acaaaagatt ccgaagaagg cttaacacat atactctcag agcatttcga gaatgttaaa
      961 accaaggttc aaggtactgt agtaatgttt tccgcttcag ggaaaaaata gcatccaacc
     1021 gcagcacgtt cttgcttaag acgtgctacg gtttccttta cgatttgggg ttgcgacttt
     1081 acctctattg ataatgcatt ccggcagaac gttcaaatat gaacgtacga tatttaacta
     1141 accgaaaaac aaaatatagc acaggcgaga catttaccat ctaagaaaat agtatcgttt
     1201 ttttacagtt aaatcaacat cacttcctta aaatgaaaac aataaatttc aatcctgaaa
     1261 cattttattt cagcttatta ttccccagta tcacatttta tcaggatatc cttctgcgag
     1321 gttattatgc ttcctgtaaa taatcccccc ctatccactg gaaacgtctc tttttacaga
     1381 actacatcaa tcgacaatgt tcacaataat tatctctccg aatgggttga atggactaaa
     1441 aacagcattt ccggagaaaa cagggaaact gcttttaccc ggctccaatt atgtctggag
     1501 aacagtgaaa catcgttgga cttatcttgt ttaggtctca gatctctacc acgattgcct
     1561 gacaatcttg atgaaattaa tgtaagcaat aaccaactat caatgctccc cgagctacca
     1621 agggcattga aagagctgaa tgcaagcagt aatcaattat ctgcacttcc tgaattacca
     1681 gtgtcgctgg aatatataaa tgtgagtgat aaccatttgt tcgcacttcc tgaattacct
     1741 gcgtcactag aatatattaa tgtaagtgac aatcacctgt ctgtacttcc gaggttacca
     1801 atgtcattgg aattacttga tgcagccaga aatgctttgg aagtaatacc agattttcca
     1861 gaaagagatg atcatattat aagaatattc tggcttaatc agaaccggat cacggcaatt
     1921 ccggaaagca tacttggcct cagttctgat agcgttgtca atcttagaga aaatcaacta
     1981 tctcccagaa taatgcaaac tttgtt
//
LOCUS       DAZXUK010000427         1995 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZXUK010000427 DAZXUK010000000
VERSION     DAZXUK010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.430"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(244..1248)
                     /locus_tag="ACH3L8_004238"
                     /pseudo
     CDS             complement(244..1248)
                     /locus_tag="ACH3L8_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            1233..1610
                     /locus_tag="ACH3L8_004239"
                     /pseudo
     CDS             1233..1610
                     /locus_tag="ACH3L8_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1649..>1995)
                     /locus_tag="ACH3L8_004240"
     CDS             complement(1649..>1995)
                     /locus_tag="ACH3L8_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFT4087085.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT          457 a          478 c          549 g          511 t
ORIGIN      
        1 ttgtatgaca gatgtaacaa aatgaaaaaa aagataagca caagtgattg attggttaga
       61 tattgatcac ttggtgtttg tattttgaat tttgcgatca aaatttgatc tttgttgttt
      121 ccggtcaatc ctttacaggt tttctgtatt atccggggga gttaagtctg tcgatgtccg
      181 acagactttt gatgatggaa acaacatgca gcgataatgt gcatgctgtt ttgcaggtgg
      241 aactcagaag gtgaactgga actgtgcgac cgcaccgtaa gtgttgtccg tgccgatcag
      301 gcctgcaaca tcaaggtgaa ccacatcaaa cggtgagatc cccaccccgg ccgtgaatgc
      361 actgccgtcg ttaccggcca gattctgacg gtaaccggcg cgcagctgtg cccatttcca
      421 ggcattaaat tctgtgccaa tggcggcaaa ctgacggttg ctgtcggagg tgaaaccgct
      481 ggccggggtc agatccacat caaatgcagt ggtgaacatc gcgttgtgcc aggagacacc
      541 ggccgtcacc tgcgggcgga tcctgaaggt ttccgtgata ccgttcactt cttttgtctc
      601 aatactacgg gagatcaggt tctgtgcgac cagtcccagc gtccagttgt catccaggtc
      661 cgtactggca ccgatgtcgg cgttgatgcc atttttcgtg ttgtggtagc ggtcgccttt
      721 gaaggcgctg ctgtcataat ttctgaccag cacgttgtag ttgaacaggt cgacgcgctg
      781 gaatttgggg gggaagccca gagaccattt ctgcccggcc gtttccagtt ccgtagccag
      841 agcaataccc acatcagtga tcagtgccgt gcgggcacag gcctgtgatg tcaggcggct
      901 tttgtccacc tcctgcccgg tgctgtttgc cacctcttcc aggtaagtga ggtcagcatc
      961 agagacttta ccgtcgacac tgacggtgcc gtgtgattta accatcagtg cggcagagag
     1021 gcgatcaccg ggaagggccg ccacagcaga aacgcccagc tgtgcggcgg catgtgtgtt
     1081 acggaagtcc cggaggtgtt ctttcaggcg tgcggcagcc tcaggaacac cgtggttact
     1141 ttcgatggcg cggtcgaagg ccttccactc atcactgatc cggtcggcgt tgtcggtgat
     1201 attatccgga ttggagagct gcgcgccgac ggacggaagc aggatgctgg gctcatgcgc
     1261 gacgcaaaat ccacgatgta tatatcagta ctaaaagcgc gacggcggaa gaagctctga
     1321 aactaatcgg tgaactgtac gccatagagc acgatatacg cgggttaccg gtgtctgaac
     1381 gcctggcggt caggcaaatg cagagtagac cgctcctgac ttccctgtat aagctgatgc
     1441 aggagaaaga acacacgtta tcgaagaaat gtcgtctggg agatgcgttc cggtatatca
     1501 ggaagcactg ggctgcgttg tgcaacttct gtgatgatgg cctggctgag gtagataata
     1561 atgcagcaga aagagcgctt cgtgcagtct gtctcgggaa gaaaactttt tcgaggatca
     1621 ctcattccgt gtctgactgc ttcagggttt aattttgctt ctgctgactg aatgatgatt
     1681 gggcagcaag tgcaatatcc cacataaatc cagtcaactc acgagccacc gccgtaatgg
     1741 ctacttgtga ttttttccct tttgctgtca gagttcggta acgagaacac aatcgttgtt
     1801 gagctttcca tgcaatatct ttggaatatt gtgttacaga gtccggtcgg taatatataa
     1861 gccatgctcc aacttttgca ggtgtacgat aagaccaggc ggcttcgtaa agcagacgtc
     1921 tcagttcgct gtttccaact tttgttattc ctctgggacg aatactgttt ccactggaat
     1981 attcacctgg cacca
//
LOCUS       DAZXUK010000428         1994 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZXUK010000428 DAZXUK010000000
VERSION     DAZXUK010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.431"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1068
                     /locus_tag="ACH3L8_004241"
     CDS             <1..1068
                     /locus_tag="ACH3L8_004241"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFT4087086.1"
                     /translation="FFWQLRSFKLDVRGYARHFKVLNSCLFALPPIAEQERIVEKVSS
                     LMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQNTAELAENWARISEHFDTLFTTEA
                     SVDALKQTILQLAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPIS
                     DEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYLNLYQVAPDIIEKGTGRVISKRTVK
                     ESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYNGLCSADMYPLDCYINPNYLLKYIL
                     SIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPPYNEQQAIFDKINSIEAVCNGLISY
                     IGIYHKTQLHLADALTDAAIN"
     gene            1289..1630
                     /gene="symE"
                     /locus_tag="ACH3L8_004242"
     CDS             1289..1630
                     /gene="symE"
                     /locus_tag="ACH3L8_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFT4087087.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(1677..>1994)
                     /locus_tag="ACH3L8_004243"
     CDS             complement(1677..>1994)
                     /locus_tag="ACH3L8_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HFT4087088.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT          618 a          449 c          397 g          530 t
ORIGIN      
        1 tttttttggc agttacgatc atttaaatta gatgtaagag gatacgcgag acattttaaa
       61 gtattaaata gctgcttatt tgcattaccc cccattgcag aacaagagcg aatagttgaa
      121 aaagttagct ctttgatgtc cctctgcgac caactagaac agcaatccct gaccagtctg
      181 gacgcacatc agcaactggt tgaaaccctg ttgggaacgc ttacagacag ccaaaacacc
      241 gcggaactgg ctgaaaactg ggcgcgtatt agcgagcatt tcgacaccct atttaccacc
      301 gaagccagcg tggatgcgtt aaaacagacc attctgcaac tggccgtaat gggcaaactt
      361 gtgccgcagg atccgaatga cgaacccgcc tctgaactgc tcaaacgtat tgcgcaggaa
      421 aaagctcaat tggtgaaaga agggaaaata aaaaaacaaa aaccactgcc gccaattagc
      481 gatgaggaaa aaccgtttga acttccggaa gggtgggaat ggtgtcgtat tggtaatata
      541 gttaatatta aatctgaatt agtatcacca aaagattatt taaatttata tcaagtagca
      601 cctgatatta ttgaaaaggg cactgggaga gtgatatcaa agagaacagt gaaagaatca
      661 ggagtcaaag ggccaaatag tcgcttttat aaaggacaaa ttgtttattc taaaatccgc
      721 ccgtcattat ccaaagtttt tttagcagag tataatggtc tttgcagtgc tgatatgtat
      781 ccattagact gttacattaa tccaaattac ttattgaaat atatcctttc cattccattt
      841 cttatgcaag tcaaaaaagc agagaacaga ataaaaatgc caaaacttaa ttctgacagt
      901 ttttacaata ttattgttgc gattcccccc tataatgaac aacaagcaat atttgataag
      961 ataaattcta ttgaagcagt atgcaatggt cttatttctt atattgggat ttatcataaa
     1021 acccaactgc atcttgcaga cgcactcact gacgcagcga taaactaatt ccctcgccct
     1081 cacaaaacca ataaagcggc taaatagccg ctttattcac atcaccaaaa tttatatttc
     1141 acgctaattt tcatcttatt ttcattaatt tttagctgga ctttccctgt atttactgat
     1201 gatgtatcca ggtatttagc acggtgcgga tgtgcgccaa cacactcgca ccgctaatca
     1261 caatcactat ttatagagaa tagtaatcat gactgacacg cattctattg cacaaccgtt
     1321 agaagcagaa gtctccccgg caaataatcg tcaattaacc gtgagttatg cgagtcgtta
     1381 cccggattac agccgtattc ccgccatcac cctgaaaggt cagtggctgg aagccgccgg
     1441 ttttgccacc ggcacggcgg tagatgtcaa agtgatggaa ggctgtattg tcctcaccgc
     1501 ccaacccgcc gccgccgaag agagcgaact gatgcagtca ctacgcaagg tgtgtaagct
     1561 gtcggcgcgt aaacaaaggc aggtacagga atttattggg gtaattacag gtaaacagaa
     1621 agtcgcctga tacattgacc cggtagcgcc agtgctaccg ggtattattc gccgcgttat
     1681 accaacgcgc tctccatctg tttttcaaat gcctgacgaa ttaatcccat aatgtaagga
     1741 agttgtgcga ggtcgctgaa accaatttct acatcgccat taccccagcg cccaacattt
     1801 gtcacatctt tggcaatacc tttcggatcg accagttcat gaaactgcat attaagcgac
     1861 aatcgcagtc ggcttttttg cggcacaaca tcaacaaaat tcgtttccgc tttaaacgca
     1921 atatatagct tcaatacttc ctgcgtgatc cctgcgtcca ggcgcataac ttcatcgcga
     1981 agatgatcga ataa
//
LOCUS       DAZXUK010000429         1984 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZXUK010000429 DAZXUK010000000
VERSION     DAZXUK010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.432"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(31..107)
                     /locus_tag="ACH3L8_004244"
     tRNA            complement(31..107)
                     /locus_tag="ACH3L8_004244"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71..73),aa:Arg,seq:tct)
     gene            complement(209..284)
                     /locus_tag="ACH3L8_004245"
     tRNA            complement(209..284)
                     /locus_tag="ACH3L8_004245"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(249..251),aa:Ile,seq:cat)
     gene            complement(429..1250)
                     /locus_tag="ACH3L8_004246"
     CDS             complement(429..1250)
                     /locus_tag="ACH3L8_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFT4087089.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(1265..1621)
                     /locus_tag="ACH3L8_004247"
     CDS             complement(1265..1621)
                     /locus_tag="ACH3L8_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFT4087090.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(1634..>1984)
                     /locus_tag="ACH3L8_004248"
     CDS             complement(1634..>1984)
                     /locus_tag="ACH3L8_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFT4087091.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT          482 a          568 c          429 g          505 t
ORIGIN      
        1 tctgccggaa ggaatctgga agaatgaata tggcgcgctg tactggattc gaaccagtga
       61 ccgattgctt agaaggcaat tgctctgtcc ggctgagcta acaacgctga ataccgataa
      121 tggaccgcca tcggggaccc gccccccgca ccaacaaccc tgttatcgtg tcgtctgctc
      181 ttcctgataa gctaatggcg gtttgtgatg gtggcccttg ctggatttga accagcgacc
      241 tggcgattat gagtcgctcg ctctcaccac tgagctaaag ggccgggagc agaataataa
      301 cggtccgtaa ttaatttcgc aataaaaaaa cccgctcagc ggcgggttgt agaaactctt
      361 ctaacgccag gcataaaacg cccatcatta tgacgaattt accacagatt ccggaaaaat
      421 caaccttgtt acctagttac cttttttaac tgccgctcag cccatgcttc ttcaatatca
      481 aaccgggtca ccagcgcatc ataaaatttc ttaactgttt tttcccatga cgcgcgtgtt
      541 atctggtttg tcacctcgca tatagcatta aatgcctccg ttgatggtag tctttcatag
      601 ccacgaccac cacaacgctg gcagtctctg ataacaggca taccacgttt taccgactct
      661 tcacgatgaa tggcgacacc acgcccacgg caatccttac aggcggtgga aacctcaccc
      721 tttccgccac actccggaca ggcaactttt accacctccc tgactttttt ccattcttcc
      781 cagtaaaacg gatacacacc tttcgtacac tttgcccata ccggcggctt accatccgga
      841 tactggatct tgtttgtaaa aactatgctt tcaataaatt tttccccata gcaacaaggg
      901 cactgctttt tactcgctgc gctgcgggca taatcctcaa aagcgtacga agccataatg
      961 cgcatcacta ccggttttat ttctgccgga agttttctca acgccgccac acgatcgcac
     1021 cgactgagtg cataatctgc cagtaattct gttgcccgcg ccctgtcatt catactgatg
     1081 cccattttcc ccaggaacgc agaaaaaccc atctcagccc gattctgtgt catgccctgc
     1141 gcggccatca catcagtgat actcagcgca tcttttgacg ttgaggccga tgcatcggtc
     1201 aggccagggg attttgggga gtagtatttc ggtaaatctt ccagtttcat tttttgacct
     1261 gctcttaatg cattatgggg taaatcttca cccccagacg tccaccagat actggctgac
     1321 cacgaacgat attgatttca tcaaactgct catcgtccat taacactccc gcatgcgtca
     1381 gcgcatccag cggtgctttc aggatattgt ccaggtcgcg acgacgctta tccggtggct
     1441 ctgcaatcac ctttatcgcc agccttccgg acaggcttaa tttcagccgc tgctggcgaa
     1501 caataagcgc cacagcccgg cgataacgct ttccctcctc cgagataaaa tatgtgctgc
     1561 cacggcgtcg ccagtaagtg ttcaccgtcg gcgggtaagg taaaaccaaa tctatgagca
     1621 tcagtcacct cttttaccca agcacgccag ttgcaaaggc gtgatcaaga aaacgaaaaa
     1681 ttaaatcaac ctgagaacca tgcttttctt cgaacgccag aggatccgca tgaagctcgt
     1741 tgtgatgctc ccgacacagc ggtagcgtga aaatatcgtg agatttggtc cccattccgc
     1801 cctgaccatg accaatcagg tgatggggat cgtcggctgg cttaccacaa cacgcacacg
     1861 gctgtgtctt cacccagcgt ttgtatttct cgttaaccca gcggcgacgt ttaggtcgtt
     1921 tcatgaaaga ttccggagac tcaggatcaa cggcaatgct gaccaccgtc ttttcctgtt
     1981 gtgg
//
LOCUS       DAZXUK010000430         1983 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZXUK010000430 DAZXUK010000000
VERSION     DAZXUK010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.433"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..355
                     /locus_tag="ACH3L8_004249"
     CDS             <1..355
                     /locus_tag="ACH3L8_004249"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFT4087092.1"
                     /translation="RKGCPNYPPEFKQLLVAASCEPGISISKLALENGINANLLFKWR
                     QQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFRHGTLRFNGN
                     VSEKLLTLLIQELKR"
     gene            352..699
                     /gene="tnpB"
                     /locus_tag="ACH3L8_004250"
     CDS             352..699
                     /gene="tnpB"
                     /locus_tag="ACH3L8_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFT4087093.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            749..>1983
                     /locus_tag="ACH3L8_004251"
     CDS             749..>1983
                     /locus_tag="ACH3L8_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFT4087094.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERG"
BASE COUNT          446 a          558 c          580 g          399 t
ORIGIN      
        1 gcgaaaaggc tgccctaatt atcctcccga atttaaacag ctgctcgttg ctgcctcctg
       61 tgaacccggg atatccatct caaaacttgc tcttgaaaat ggcattaacg ccaatctgtt
      121 gttcaaatgg cgacaacaat ggcgcgaggg aaagctgcta ttaccttctt cagagagccc
      181 ccagctactt cctgtgactc tcgatgcagc tgccgaacag ccagaatcgc tcgcagagga
      241 cccggaaacc ctcagtatca gctgtgaggt aacgttccgg cacgggacgc tccgcttcaa
      301 tggcaatgtc agcgaaaagc tcctgactct gctgatacag gaactgaagc gatgatcccg
      361 ttaccttccg ggaccaaaat ttggctggtt gccggtatca ccgatatgag aaatagcttc
      421 aacggcctgg ctgcgaaagt acaaacggcg ctgaaagacg atcccatgtc cggccatatt
      481 ttcattttcc ggggccgcag cggcagtcag gttaaactgc tgtggtccac cggcgacgga
      541 ctgtgcctcc tgaccaaacg gctggagcgt gggcgcttcg cctggccgtc agcccgtgat
      601 ggcaaagtgt tccttacgca ggcgcagctg gcgatgctgc tggaaggtat cgactggcga
      661 cagcccaagc ggctgctgac ctccctgacc atgctgtaaa tctctttatc ctggttgtca
      721 cagaataagc ccggtaaaat acgggcttat gaacgacatc tcttctgacg acatcttcct
      781 gctgaaacag cgcctggccg aacaggaagc gctgatccac gccctgcagg aaaagctgag
      841 caaccgggag cgcgaaatag accatctgca ggcgcagctg gataaactcc gccggatgaa
      901 cttcggcagt cgttccgaaa aagtctcccg ccgtatcgca caaatggaag ccgatctgaa
      961 ccggcttcag aaagagagcg atacgctgac tggtagggtg tatgacccgg cagtacagcg
     1021 tccgttgcgt cagacccgca cccgtaagcc gttccctgaa tcactacccc gtgacgaaaa
     1081 gcgactgttg cctgcggcgc cgtgctgccc gaactgcggc ggttcactga gctatctggg
     1141 cgaggatacc gccgaacagc tggagttgat gcgtagcgcc ttccgggtta tccggacggt
     1201 acgggaaaaa catgcctgta ctcagtgcga taccatcgtg caggcacctg caccttcgcg
     1261 gcccatcgag cggggtatcg ccggaccggg gctgctggcc cgcgtgctga cctcgaagta
     1321 tgcagagcac accccgctgt attgccagtc agaaatatac ggccggcaag gtgtggagct
     1381 gagccgttca ctgctgtcgg gctgggtgga tgcatgctgc cggctgctgt ctccgctgga
     1441 agaggcgctt catggctatg tcatgactga cggcaaactc catgccgatg ataccccggt
     1501 ccaggtactg ctgccgggta ataagaagac gaagaccggg cggttgtggg cgtatgttcg
     1561 tgatgaccgc aatgccgggt cagcgttggc acctgcagtg tggttcgctt acagcccgga
     1621 cagaaaaagc atccatccgc agactcatct tgcttgcttc agcggtgtgc tgcaagcgga
     1681 tgcgtacgcc gggttcaacg agctgtatcg caatggtggg ataacggaag ctgcctgctg
     1741 ggctcatgcc cgccgaaaga tccacgatgt gcacgtccgc atcccgtcag cactgacgga
     1801 agaagccctg gagcagatcg gtcagttgta cgccatagag gcggatataa ggggaatgcc
     1861 ggcagagcag cggcttgctg aacgtcagcg aaaaacgaaa ccgctgttga aatccctgga
     1921 aagctggttg cgtgaaaaga tgaagaccct gttcttcggc tctggccatg gtggtgagcg
     1981 ggg
//
LOCUS       DAZXUK010000431         1975 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZXUK010000431 DAZXUK010000000
VERSION     DAZXUK010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.434"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..836)
                     /locus_tag="ACH3L8_004252"
                     /pseudo
     CDS             complement(1..836)
                     /locus_tag="ACH3L8_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          532 a          465 c          456 g          522 t
ORIGIN      
        1 gctgctctac ggtcatattt ttaattgcgc tccggtttac aagagcccac ccctgttttt
       61 ccagataaaa ctggaaagta tccagggtac agaccagtgc accgtcagga acagtttcgg
      121 tgaatctgat attgccgtgt tcgtcgaagt gcacaaccag agtgcgaccg ttacccggaa
      181 tcatcttgtc agcggacggg gtattattct ggtgcagttc tgcctccatg cggtcgaaca
      241 tcagcgatgt aggcctcttt gaatgcagcg gcttttttcc cagtgaagcc catcaccagg
      301 aaaacgaagc cgtttttggt gatttggtac attgggcgtt tttcgccttt ggtgtcggtg
      361 taggtgacgt cctcaaaatt gagggcgtta aattcaggtg agcattcagt atttgcgatg
      421 gcacgtaaca cattgtcgtg tcgtttgtgg aagaactctg caaccgcaac agacgtagtg
      481 acagcgcgac cgttttcgat ggttacgtca gggtgagaaa gggcagggat agtagccatg
      541 atggcatcct cgagtgataa gttaattaac tcaccaccga ggttttccac gaccataagg
      601 gtggtgagac gtacaggggt ggaaataccg gtcactcgag aacccggcca gtcttgcgac
      661 tgccctgcac atcccaccat aatttgaatg gagctgtgca ttacgcataa aaaaaccgct
      721 tcagcgcggc tatgcgctcg agtaacgttc gggtttccac gcccggcccc cgttttatga
      781 ggtgcagatg cactataatt ccacccgttc tggttttcaa tagctacatt caacattttc
      841 tcttaccttt catcaccgaa gtgaactttg ttgatgcggt gcctggtgcc tccaggtgac
      901 gttaaccagt taacaattaa cgccggatac agggaaaccc ataaccaccc gatacgtggg
      961 aactaatcgc tttttaactg ttccgcgtgc gctcagccgc attcaccgca tcacaaaatt
     1021 cactttaaaa agggcggaca tcagccagca atgaaactga tgccgccaaa ggtaccaatc
     1081 aacatggagt gttgtggcgt ggttgtcact taagcgtatg gtcaacctga caacccggtg
     1141 tcctcaacgg ggaaggaata actccgccat acttaccgcc gcgtcatttc gcgttgtgtg
     1201 cctgctttta actacgtcag gcgaggtggt tcctgttatt ccccaacaac aagaaatttg
     1261 tataatctga ataccccaac aataagaaga gtgagcaagt gatgtattag cgatgtcatc
     1321 tctcaaagag actgcatcaa tgttaaaatt attcaatgaa gcaaaaactc aggctgaggt
     1381 tgatcgcgct gtgtctgaaa taaactctaa gttgtcaagc attcagcttg aatatttgtc
     1441 atctcattga tgagctgaca atctctaaaa gcaatgaggc tgcgctcaaa gcaaaactca
     1501 cggagataga aaagtttaag ttccaaagtg aagactgcga acctcgctaa ttgattactg
     1561 gaacgattgt gtatgccaga cagtgcaata ctagctctgg caagattacc atgtattttt
     1621 gtccagaatg ttttgcgaag agagcaatat ctatattgca acctttccct atcgaggaag
     1681 gcgataaata tacgaaaact caatgcccct cctgtaaaaa tgagtatctc atggactaca
     1741 atttcaataa tgatttttga gtcacggtat tcagattttt aaagaacatg ccggatactc
     1801 acccgtgtcc ggcgcacgca ctccacctca cccgtggaga actccttaat taccaacctt
     1861 ggcttcgttg gttagccatt aacgctggta tgtaatcatt ctggcaatgc ttaatttgaa
     1921 gtggcacact gaatttggcc acctgaacag aggtgatatg ctcacctcag aacaa
//
LOCUS       DAZXUK010000432         1972 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZXUK010000432 DAZXUK010000000
VERSION     DAZXUK010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.435"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..414
                     /gene="torD"
                     /locus_tag="ACH3L8_004253"
     CDS             <1..414
                     /gene="torD"
                     /locus_tag="ACH3L8_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFT4087095.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACH3L8_004254"
     CDS             complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACH3L8_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFT4087096.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACH3L8_004255"
     CDS             complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACH3L8_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFT4087097.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT          467 a          507 c          502 g          496 t
ORIGIN      
        1 acgctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt
       61 ctgatgaccg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa
      121 gagattaaac gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaaccg
      181 gcagatcatc tggcgatcta tctcgagttg ttgagttatc tgcatttttc gctgggagag
      241 gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg
      301 caatggttac cagagtttgc tgcgcgttgt cgacagtatg acagctttgg tttttacgcg
      361 gcactaagcc agttattgct ggtgttagtg gagtgcgacc accaaaacag ataacgtcgt
      421 ttatgcgcct gaaaagacgc gtttagcgtc gcatcaggca ttatggcgca gttgccggat
      481 gcgtcatcac atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg
      541 cgtagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc
      601 gcgatccccg gccagtccag agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca
      661 atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc
      721 cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg
      781 gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat
      841 caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt
      901 tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca
      961 gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca
     1021 aaatgctttc tttcagtgtt ggaacggtga ctttagtacc cagcgccgct tcccacgggc
     1081 taaccggcac cacaatttcc agatcctggc cgacaatatc gaacagcgga tgtggcgcaa
     1141 tatgaatcac cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc
     1201 ctttcaggcg gatgcgttga ccattgccga cgcctgccgg gatcttcaca ttcagcgttt
     1261 tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg
     1321 tacgcttatg ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt
     1381 ggccacgtgc ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga
     1441 agatatcgtc aaaatcttcg gcattaaaac tctgaccgtc gccatgatgg aactgacggt
     1501 taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat
     1561 cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt
     1621 tgctgacatc aggatggtat ttacgggcca gtcgacgata ggcggtcttg attgtcttga
     1681 gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat
     1741 ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg
     1801 taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga
     1861 ggtcgccatc aagatgggtt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt
     1921 tttgatagtt gaaccaggca ctttaagttt aactagggcg tcattattta tt
//
LOCUS       DAZXUK010000433         1935 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZXUK010000433 DAZXUK010000000
VERSION     DAZXUK010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.436"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACH3L8_004256"
     CDS             complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACH3L8_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFT4087098.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(590..1255)
                     /locus_tag="ACH3L8_004257"
     CDS             complement(590..1255)
                     /locus_tag="ACH3L8_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFT4087099.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(1424..1780)
                     /locus_tag="ACH3L8_004258"
     CDS             complement(1424..1780)
                     /locus_tag="ACH3L8_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087100.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            complement(1838..>1935)
                     /locus_tag="ACH3L8_004259"
     CDS             complement(1838..>1935)
                     /locus_tag="ACH3L8_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFT4087101.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
BASE COUNT          466 a          502 c          430 g          537 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccacccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tcacagaggt ctttcctcgt taccagtgcc
      301 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      361 atagcctgct tctccttgcc tttcggcatg taagaggcta acctacatgt gcaaagcatg
      421 aaattggcct cagattaatg ttaagcgtct tgccggacgc gtaatgttaa ctggggcttt
      481 tctctatctg ccttttggtg ttcatgcctg aagcagatag cctcaagcac ccgcagtcat
      541 tctacttacc tcgccaatat gaaatcaatc agaaaggtgc cccataaaat cactccttct
      601 cttctttacc gtagtggagt tgaccaattt tgataagagg gcgtccctga gatttgcggt
      661 gtagattggt atcgcgcaga gaatacacac agccacaata ttcctgctga tagaattttt
      721 cgcgcttgct gatttcaatc atacgggacg agccgccctg cttgcgccag ttataatccc
      781 agtacaccat gcccggataa tgcgcgacgg ctcgccgccc acagtcgtta acctgctgca
      841 tatttttcca gcgtgaaatg cccagtgaac tgctgatcac actgaaacca ttttcagcgg
      901 cgtacagcgc tgtccgctca aaacgcatgt caaaacacat ggtgcaacgg atccctcgtt
      961 cgggctccca ttccattcct ttggctcgtt caaaccagtt gtctgtgtcg taatcagcat
     1021 caataaacgg cacgccgtgt tgttcagcaa agcgaatatt ctcatcctta cgaattaaat
     1081 actctttctg aggatgaatg ttcgggttgt agaaaaagat ggtgtagtcg atttccgagg
     1141 cctgaagcgc ctccatcacc tcaccggaac atggagcaca gcaagagtgc agtagtagtt
     1201 tgtttgcccc gtttgggagc tccaatttgg gccgtttgaa atcagcaact gtcataaata
     1261 tgtttattga ggtcataaaa atatcaaaga gtgtagcatt agaacggggc tatcggaaac
     1321 agatgtgtga ctgctccccg ccctttcggg cggtctcctg atgatttgag ggtgcagaaa
     1381 tccctccggt taaggattaa atttttaaca gtgctaaatt taattattca gttctggatt
     1441 ttgtcgccct gcgtatccgc gctttcgcgt tacgctcaat ctgtatcagc ttttctatat
     1501 ttctccgcct ttcctgttcc tcctggcgca atagccttac atcatctgcc agtctggttt
     1561 ctctttttgc cacagagagc atccagtcaa acggctccac aaccgcaccg cagattttac
     1621 agcggacctg acgctctttt tcgtcaaccc gtacagaggc gtgatggcaa tatggtcttt
     1681 ccgatggctt ataaagaaaa ttaacctgat tacgagggtc atcctctttt atcggaaata
     1741 aaacgatatt gcttaactca tcttctggtt ttatttccat gctcctctcc tttgatgcga
     1801 atgccagcgg taattgaagc ctgatagcta atttcattca cagtatcgcc tcctgaaaat
     1861 taccctgata gaaagccagt acacgctgca tagcttcgct cttccggcac tcgcgacaga
     1921 ttatattcag gcgcc
//
LOCUS       DAZXUK010000434         1929 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZXUK010000434 DAZXUK010000000
VERSION     DAZXUK010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.437"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            343..549
                     /locus_tag="ACH3L8_004260"
     CDS             343..549
                     /locus_tag="ACH3L8_004260"
                     /inference="COORDINATES: protein motif:HMM:NF018642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087102.1"
                     /translation="MPVTTITPVSDAGGLQDFIYWRPDADRAGMEPVYVILNDPLDSG
                     RFTRKQLNKNILNMQVILVFSIQE"
     gene            747..>1929
                     /locus_tag="ACH3L8_004261"
     CDS             747..>1929
                     /locus_tag="ACH3L8_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4087103.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AA"
BASE COUNT          508 a          380 c          439 g          602 t
ORIGIN      
        1 gggttgggga gcaatggaac agtaaacgcc gttaagaagt caatttaaat agccaaaaat
       61 taactagcca atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa
      121 ttacctatac ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt
      181 tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg
      241 ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg
      301 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
      361 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      421 agggctggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      481 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      541 caagaataaa tagtagtaac tactatagct gcaaataatg ttaaaaacat ttctatgatc
      601 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      661 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      721 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      781 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      841 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      901 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      961 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
     1021 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
     1081 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
     1141 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
     1201 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
     1261 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1321 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1381 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1441 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1501 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1561 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1621 aggatatggg aaaactggca tcaatggggg ggggttctgt ttttgaaatc ggtggtgtgg
     1681 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1741 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1801 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1861 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1921 gagccgcat
//
LOCUS       DAZXUK010000435         1921 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZXUK010000435 DAZXUK010000000
VERSION     DAZXUK010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.438"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            6..629
                     /gene="tetR(B)"
                     /locus_tag="ACH3L8_004262"
     CDS             6..629
                     /gene="tetR(B)"
                     /locus_tag="ACH3L8_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFT4087104.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(607..1293)
                     /locus_tag="ACH3L8_004263"
     CDS             complement(607..1293)
                     /locus_tag="ACH3L8_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFT4087105.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1301..1687)
                     /locus_tag="ACH3L8_004264"
     CDS             complement(1301..1687)
                     /locus_tag="ACH3L8_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HFT4087106.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            complement(1680..>1921)
                     /locus_tag="ACH3L8_004265"
     CDS             complement(1680..>1921)
                     /locus_tag="ACH3L8_004265"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFT4087107.1"
                     /translation="LLSHVAGFISIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLH
                     AKAQQEGRESIFDFYKISIAHITREYSFKKDKDNV"
BASE COUNT          584 a          412 c          353 g          572 t
ORIGIN      
        1 taatgatgtc tagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg
       61 aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc
      121 ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga
      181 tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt
      241 tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag
      301 tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct
      361 ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc
      421 attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg
      481 aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc
      541 accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa
      601 aacaacttaa atgtgaaagt gggtcttaaa agcagcataa cctttttccg tgatggtaac
      661 ttcacggtaa ccaagatgtc gagttaacca ccctttagat tcataaagcg aaaataatgc
      721 ggctccaacg tacccaccta aatggaaacg gcgttcactc caatctaaac acgcacaaca
      781 gattttacgt gaatgtttgg aaggaacgtc aattcccatt tcatgaaaat attgaatacc
      841 acttaatgtg atcattgaac cattttcagt gatccattgc tgttgacaaa gggaatcata
      901 gatcttaacg gcaacttcgc cagctaaatg atcatagcaa gtacgtgctt ttcgtaaatg
      961 cactggcgtg gaaactttgg catgtacgcc atggtttaag gagatcccca tcatactttc
     1021 catcaattca gcaatatctt ttcctgctag ccgaaaataa cgatgcttgc cttgagctac
     1081 tactgtgatt agctggcaat ctaataattt agataaatga ctgctcgccg ttgaagctga
     1141 tatattcgcc acagaactta gctcagtggc cgtccaagct cgcccatcca tcaaagcact
     1201 gagtatttta actcgtgaaa tgtcagacat agccgcccct atcgcggcta ttgaggactc
     1261 aaaggtaacc tcttttcgta ttaaattagc catcgcaagt tcactttatt gcccaaggga
     1321 gcgtaacaga tgcagccata ctatcattgt ccgttattaa tatcagttgg ttagcatggt
     1381 cactgtattg cactaaaata ttaatgttat tctccgccaa tactcgtgct atttcgccaa
     1441 gttcccccgg tttttcctgt tttaacttac gaattaatgg tgtccggatc gcaagtacta
     1501 acagtccagc ttgctctagc gctattttag cttcctttcc ttgttcaaca agaaaatgag
     1561 catggcattc atcaccaacc gtaaatatcc ctcccccttc caatccaata cctttattac
     1621 ctaatgtttt tcctagtaat gctaattgtc ctatttgatt atctaaaaca acgtgaacat
     1681 caaacattat ccttgtcctt tttaaatgaa tattcgcgag taatatgagc aatactaatt
     1741 ttgtaaaaat caaatattga ctctcgccct tcttgttgag ctttcgcatg taaaacatga
     1801 tttttccatt gcagaactgc gtattcgttt tcccaccaag atagcgataa catttttcct
     1861 tctgtagcta gactttgaaa acgttcaatt gaaataaaac cagctacatg acttaatagt
     1921 g
//
LOCUS       DAZXUK010000436         1913 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZXUK010000436 DAZXUK010000000
VERSION     DAZXUK010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.439"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(53..1702)
                     /locus_tag="ACH3L8_004266"
     CDS             complement(53..1702)
                     /locus_tag="ACH3L8_004266"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFT4087108.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAZXUK010000437         1893 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZXUK010000437 DAZXUK010000000
VERSION     DAZXUK010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.440"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            47..283
                     /locus_tag="ACH3L8_004267"
     CDS             47..283
                     /locus_tag="ACH3L8_004267"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4087109.1"
                     /translation="MSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVRPWP
                     KGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            258..1889
                     /locus_tag="ACH3L8_004268"
                     /pseudo
     CDS             258..1889
                     /locus_tag="ACH3L8_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          510 a          415 c          523 g          445 t
ORIGIN      
        1 gttttggtaa ccctttatca tacgcaatat ggttggtgtg cgaaagatga gtaacgattg
       61 gcatcgggcc gacgtgattg cggccctcaa aaaaacagga acccctctgg caaaattgtc
      121 cagacgttca ggttattcaa ccgggacgct tgcaaatgca ttagtcaggc catggcctaa
      181 aggtgaacgc attattgctg acgccttaaa catgaaacct gaggaaattt ggcctagtag
      241 atatctaagg gatcacaatg agagagtgga tatcagcaat tgaatgcatg ggtttatctg
      301 gtttacctgg ttcattaatg ggggttcaca aaagagctca gagagaagga tggctcaaga
      361 gaagaagaga aggaattaga ggggttgctt acgaatacca tatatcttct cttcctaaca
      421 ttgctcgagc tgaagttttg ctccaacaag gaaaatttaa aaccagtcag ggatgttttg
      481 aaatcgcccg ccccacgctg gaagctcatg attatgatcg tgaggcacta tggagcaaat
      541 gggataaagc cagcgattcc cagcgcagcc tggctgaaaa atggttgccc tcaattcagg
      601 ccacagacga aatgctgaac caggggattt caacgaaaac ggcttttgcg accgttgcag
      661 ggcattacca ggtcagcgca tccactttgc gggacaagta ttaccaggta cagaagtttg
      721 cgaagcctga ctgggcggct gcacttgttg atggacgtgg agcatcccgt cgcaatgttc
      781 acaaaagtga atttgacgag gatgcctggc agtttctgat tgcagattat ctgcgaccgg
      841 aaaaacccgc cttccgcaaa tgttatgagc gtctggaact ggcagcccgc gagcatggct
      901 ggagtattcc ctcccgtgcc acggcctttc gccggattca gcaactggac gaggcaatgg
      961 ttgttgcctg tcgtgaaggt gaacatgcac tgatgcatct gataccggca cagcagcgaa
     1021 ctgtggaaca cctggacgcc atgcagtgga tcaacggcga cggttatctg cataacgtct
     1081 ttgtacgctg gtttaacggt gatgtgatcc gtccgaaaac atggttctgg caggatgtga
     1141 aaacccgaaa aattctgggc tggcgctgcg atgtgagcga gaacattgat tcaattcgcc
     1201 tctcgttcat ggatgttgtg actcgctaca gtatcccgga ggattttcac atcaccattg
     1261 ataacacccg tggtgctgcg aataaatggc tgacgggagg cgcgcccaat cgctaccgct
     1321 ttaaggtaaa agaggacgat ccaaaaggac tgtttttact gatgggtgcg aaaatgcact
     1381 ggacaagcgt tgttgccggt aaaggctggg gccaggcaaa acctgttgaa cgtgctttcg
     1441 gtgttggtgg gcttgaggaa tacgttgata agcatccggc actggctggc gcatatacgg
     1501 ggccaaatcc gcaggcaaaa cctgataact atggcgacca cgctgttgat gcagagctgt
     1561 ttctgaaaac ctttgccgaa ggtgtggcga tgttcaatgc cagaacaggc cgtgaaacag
     1621 aaatgtgcgg gggcaaactc tcgtttgatg atgttttcga gcgtgaatac gccagaacga
     1681 ttgtgcgtaa gccaaccgaa gaacaaaaac ggatgctgtt actgcctgcc gaggcggtga
     1741 acgtttcacg caaaggcgag tttacgctta aagttggcgg ctcccttaaa ggcgcgaaaa
     1801 acgtttatta caacatggca ttaatgaatg ccggagtgaa aaaagttgtg gtcagatttg
     1861 atccacagca gttacacagc acggtttatt gag
//
LOCUS       DAZXUK010000438         1874 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZXUK010000438 DAZXUK010000000
VERSION     DAZXUK010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.441"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..279)
                     /locus_tag="ACH3L8_004269"
     CDS             complement(1..279)
                     /locus_tag="ACH3L8_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087110.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACH3L8_004270"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACH3L8_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFT4087111.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="ACH3L8_004271"
     CDS             complement(1254..1670)
                     /locus_tag="ACH3L8_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087112.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="ACH3L8_004272"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="ACH3L8_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
BASE COUNT          474 a          464 c          410 g          526 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aatt
//
LOCUS       DAZXUK010000439         1860 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZXUK010000439 DAZXUK010000000
VERSION     DAZXUK010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.442"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..344)
                     /locus_tag="ACH3L8_004273"
                     /pseudo
     CDS             complement(1..344)
                     /locus_tag="ACH3L8_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(344..1264)
                     /locus_tag="ACH3L8_004274"
     CDS             complement(344..1264)
                     /locus_tag="ACH3L8_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFT4087113.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1402..>1860
                     /gene="pgrR"
                     /locus_tag="ACH3L8_004275"
     CDS             1402..>1860
                     /gene="pgrR"
                     /locus_tag="ACH3L8_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFT4087114.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVLRCTGNSG"
BASE COUNT          465 a          451 c          433 g          511 t
ORIGIN      
        1 cacacaccca gcacgccaat gcgttcagca tcaacatatg gcagagttac tgcataatcg
       61 ataacacggc taatatcctc tacgcgctgt gtcggatctt ctatccaacg aggctaccca
      121 ccagattctc cctggaaact ggcgtcataa gcgagaacga catagccttt ttcagccaat
      181 gccttgccat agatattgcc agacgtctgc tccttacaac tgccaaaagg atggacgctg
      241 ataatcgccg gatactgttt tgactcatca aaatttggtg gaaactgaat attggcagca
      301 atatgccagg ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa
      361 agaacggagc taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca
      421 tatggtttgc gccttcaaca atgtgatagc gtttatcctg gcttgatgcc cgatcgtaca
      481 ggtcgtcact catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag
      541 tcaggtacac ttccgccata tggtaagcat cataggtaat aatctggtta aggctgcgca
      601 aagtagcgta acccggtgct gttggatact gcgcacgagg tgtgtggtaa tactcccagg
      661 cctgacgcag ttcttcgttc ggcgcatcgg cctccttcat tggagccagt ggcataatgg
      721 cgtattcttc gctgctaata tcgctggttc gggcgtttga gccagcttca atgtgtggca
      781 gagcatcaat agatttcaca ttgttttccc aaccattgcg gaacatcgaa ccaatattga
      841 ccgcactaac ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat
      901 atcctgcacc ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg
      961 ttgtcaggta atcaatcacg gcactgacgt cttcagtacg aatgtatggg ttttctaact
     1021 gacgcggctc gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc
     1081 ccttttccgc cagcttttcg gcataggttc cggccgtttg ctctttaacg cccccacctg
     1141 ggtgagatac cacaattgcc ggatactgac gggtttcatc aaattttgga gggaaataga
     1201 tcactgcaga caaagagatg gttggattat tgctgttagt gaagctgact ttattattca
     1261 tcattcagtt cctcatggag ttgtcggttc gttttgacgg ttggtgatat cactatagat
     1321 attgatcatt aagttgatta gaccctaaat catgattaga cctatcattt aaatgattaa
     1381 tagattgtat ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc
     1441 gtcgttgcag aggagcgtag cttcactcgc gcagcagccc gcctgagcat ggtgcagtca
     1501 gctttaagcc agatagtgcg ccgtatagaa gaacgattgg gattgcggct tctgacgcga
     1561 accacgcgca gcgttgtacc cactgaagcg ggcgagcatc ttttgtctgt tcttggccct
     1621 atattgcatg acatagattc tgccctgaca tccctgagcg atctgcagaa ccgcccatcc
     1681 gggacaatac gtattactac tgtagaacat gcagcaaaaa cgatattgtt accagccatg
     1741 cgcacattcc tgaaatcgca tcctgaaatt gatattcagc ttaccattga ttatggtttg
     1801 accgatgtcg tttctgaacg ttttgatgca ggcgtcctga ggtgtactgg caatagcgga
//
LOCUS       DAZXUK010000440         1849 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZXUK010000440 DAZXUK010000000
VERSION     DAZXUK010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.443"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..257)
                     /gene="ydfV"
                     /locus_tag="ACH3L8_004276"
     CDS             complement(<1..257)
                     /gene="ydfV"
                     /locus_tag="ACH3L8_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFT4087115.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            282..521
                     /gene="relB"
                     /locus_tag="ACH3L8_004277"
     CDS             282..521
                     /gene="relB"
                     /locus_tag="ACH3L8_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFT4087116.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            521..808
                     /gene="relE"
                     /locus_tag="ACH3L8_004278"
     CDS             521..808
                     /gene="relE"
                     /locus_tag="ACH3L8_004278"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFT4087117.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            880..1035
                     /gene="hokD"
                     /locus_tag="ACH3L8_004279"
     CDS             880..1035
                     /gene="hokD"
                     /locus_tag="ACH3L8_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFT4087118.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1253..1504
                     /gene="rem"
                     /locus_tag="ACH3L8_004280"
     CDS             1253..1504
                     /gene="rem"
                     /locus_tag="ACH3L8_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFT4087119.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1571..1849
                     /locus_tag="ACH3L8_004281"
                     /pseudo
     CDS             1571..1849
                     /locus_tag="ACH3L8_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          507 a          410 c          468 g          464 t
ORIGIN      
        1 gacatgctaa agcaaaaaat agatgaataa gttgagttgt gcatatgtag cctgaccgtc
       61 acaaagtata tggtgtctgt accagtaaga tgatggccgg actctttaaa aaagagctga
      121 cctgcacaat acaggatgga cttagcaatg gctgctcctg gcacaaagcg gacagtgatc
      181 accgttctta cgactacttt ctgacttcct tcgtgacttg ccctaagcat gttgtagtgc
      241 gatacttgta atgacatttg taattacaag aggtgtaaga catgggtagc attaacctgc
      301 gtattgacga tgaacttaaa gcgcgttctt acgccgcgct tgaaaaaatg ggtgtaactc
      361 cttctgaagc gcttcgtctc atgctcgagt atatcgctga caatgaacgc ttgccgttca
      421 aacagacact cctgagtgat gaagatgctg aacttgtgga gatagtgaaa gaacggcttc
      481 gtaatcctaa gccagtacgt gtgacgctgg atgaactctg atggcgtatt ttctggattt
      541 tgacgagcgg gcactaaagg aatggcgaaa gctgggctcg acggtacgtg aacagttgaa
      601 aaagaagctg gttgaagtac ttgagtcacc ccggattgaa gcaaacaagc tccgtggtat
      661 gcctgattgt tacaagatta agctccggtc ttcaggctat cgccttgtat accaggttat
      721 agacgagaaa gttgtcgttt tcgtgatttc tgttgggaaa agagaacgct cggaagtata
      781 tagcgaggcg gtcaaacgca ttctctgaac caaagcatga catctctgtt tcgcaccgaa
      841 ggtgacactt ctgctttgcg ttgacaggag aagcaggcta tgaagcagca aaaggcgatg
      901 ttaatcgccc tgatcgtcat ctgtttaacc gtcatagtga cggcactggt aacgaggaaa
      961 gacctctgcg aggtacgaat ccgaaccggc cagacggagg tcgctgtctt cacagcttac
     1021 gaacctgagg agtaagagac ccggcgagtg agaaatccct cgccacctct gatgtggcag
     1081 gcatcctcaa cgcacccgca cttaacccgc ttcggcgggt ttttgttttt attttcaacg
     1141 catttgaagt tctggatggt gccggaatag aatcaaaaat acttaagtag cgcgcaggga
     1201 taaaagggat ggaccccgaa caggggagtg ctacttatct ggaaggattc tgatgatgaa
     1261 catcgaagaa ctgcgtaaaa ttttttgtga agatggcctc tatgctgtgt gcgttgaaaa
     1321 tggaaatatt gttagtcatt accgcattgt gtgtttgcaa aaaaatgggg ctgcgttaat
     1381 taattttgtg gatgcccgag tgacggacgg atttatcttg cgcgacggtg agtttgtcac
     1441 ttcattacag gcattgaaag agatcggaat aaaagctggc ttttctgctt tttcagaaga
     1501 ataaactcat ctacaatctt gcgcggggct gaactcccgc tgagtaacac cgtgccaccg
     1561 gagaaaaccg atggcacgca acgcaaaata ttacaatcat gataattcga ccgttcttgc
     1621 ccacacgcac gagcggtatt ctcacgcatt taagtcagac tggtaccagc atccctcatg
     1681 cactgaagaa caggccgaat ggcttattca gtgttaccgc aggcgcggat gcgaggttaa
     1741 aaaagccctt agcctcgact accgtcacta gataatctcc gtcaggctcc cttactccga
     1801 acagccaccg cgtccgtccc gcacattcca gcaacggatc tggaggtaa
//
LOCUS       DAZXUK010000441         1831 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZXUK010000441 DAZXUK010000000
VERSION     DAZXUK010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.444"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(320..874)
                     /locus_tag="ACH3L8_004282"
     CDS             complement(320..874)
                     /locus_tag="ACH3L8_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFT4087120.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(871..1161)
                     /locus_tag="ACH3L8_004283"
     CDS             complement(871..1161)
                     /locus_tag="ACH3L8_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFT4087121.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQVAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1161..1760)
                     /locus_tag="ACH3L8_004284"
     CDS             complement(1161..1760)
                     /locus_tag="ACH3L8_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFT4087122.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          446 a          496 c          419 g          470 t
ORIGIN      
        1 ttatgggtga tcggggagag agtttcaaca gagatttgag tggtcataac gataactccg
       61 tacatttgga aattatcgcc accgacgacg ccaatcgaac tggtggcgaa ctgtgcaggg
      121 ttggcgtaac cgggtacgga aaccggcgag cctttcggct cccccacaca gcccgccata
      181 aatcgcgaat gtgactgtgc aaacgatatg aaaaaagacg cgggcgcgtc tcatatcgct
      241 ccgtaaacat ccgggacgcc aatcccgacg ccagattttg ctggcgcgtg aggaatatag
      301 ccccggataa cagattgagt caacaaacgg tttttagatc cccggaagag aatgcatcac
      361 gcatcggtag atagagcata tattcagcaa gatttagcca tacatctacc cgactgcagc
      421 acgtccggaa acaccactca gggtgtgcgt cattcagcaa ttcagccatt ttacgcttag
      481 tcatcccccg cccttcatac cgttgccgga ggacactaat caatccagga tgctctgcca
      541 gcacctcact tatgactcta tcaatacata acgcctctgc atcagtacaa tgcgccagcc
      601 aggtcttttg cttgccattg atcatctctc gcaaaaacgc ttccagctca ggtttctcta
      661 ttcccgcttt tttcatcctg cgcagggctt cattgacggc tgttttcgtc aattttttgg
      721 atgccagcaa ctggttaaac atatttcccg tcttaccgcc gccaatatac gaccagcgcc
      781 cccacatgcg cagttttccc tgaatccaga cactttccag cgtggtgaga cgaaggtgtt
      841 ccccgctttt gcctgtattt gttgggtaaa tcataaataa ccttcctttc tccagatttc
      901 ttgtgtgcga aaaacacctt ctgcatgcat caggcgtaat tcttctttgg tgtaatcgct
      961 ggtttttacc cgcccgtcga ttaaatcgtg gcatgagcta caggcaatcg ctacctgcat
     1021 atcgtgtggt tttgtcgctg ttccgcacgt ccccgccagc ctgtaatgcg ccagcacaga
     1081 agtttcggga ttgtgattgc agtagccagg aattctgacg gtgcacatct gcccccgcgc
     1141 cgctttacgt aaatccacca ttacgcaaac tccagtaact gcgcggccac attttcgact
     1201 tcctccggag aggaaaattt acggaacagg atccagttcc acagcacatt cagtacagat
     1261 ttataaacct gctgaaactc ggtttcgtcc atgttcgcaa atgcgataga ttttgccctg
     1321 cgcccacgac taccatcagg ataaatatgc tcggtgtaaa atccggcctg aatggttacc
     1381 cactcgcgga aagcgtcaaa cgactttagc aatgccgtat cccgggttct gcatgtcgca
     1441 actgtattaa ggtattgctc tgcggcatca ctcagggctg gagtgtgttc ccgacctact
     1501 gattcgcaca ggtaatcaac gaagcctgat accagttttc gttcgcgagg cgtgatcgcc
     1561 ccaccgaccg gagtccagta atcaaatccc agttgcagga gtttgaaaaa acgcttgtgg
     1621 aatgcgtagt tacgcacacg cttaaagtct gcgtgtatcc actcacctat tttgatttga
     1681 tgcaaaaaat cgcaactctc cggcgtcgcc gggagaagta atccggaaga ggtttgtttg
     1741 accagttgta tatgcgccat cgtagttctc cgctggcgca gtagaatggg tgttcagccc
     1801 gttatgtagt ataccagaat taatgccaat a
//
LOCUS       DAZXUK010000442         1824 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZXUK010000442 DAZXUK010000000
VERSION     DAZXUK010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.445"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            176..1405
                     /locus_tag="ACH3L8_004285"
                     /pseudo
     CDS             176..1405
                     /locus_tag="ACH3L8_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT          500 a          385 c          352 g          587 t
ORIGIN      
        1 tttatcacaa aggaaatatg cctgagcagt agtcagagtc ataactggca cgtaaggttt
       61 gcaaccacta acccaccaat agatgggtag atagggcgtt aatctcccat acttaacttg
      121 gtttatggta aattgccctc cattttgttt aatttgtaga tgatacgttc agataatgtc
      181 taatatttgg tctaaagaag aaactctgtg aagtttcgcg ctctatggca cagccgttga
      241 tgcaggcacg ctattccttc ctattcagtt aggttcggca ggggctgtgg tcctgtttat
      301 tactgctctg gtcgcctggc ctttaacata ttggccacat aaagccttat gccagttcat
      361 cctctcatca aaaacatcag caggtgaagg gataacgggc gcggtaacac actactatgg
      421 caagaagatt ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt
      481 gatatatgca gtggcaatta ccaactcact tacggaacag ctggcaaagc atatggttat
      541 tgatcttcgc atccgtatgt tggtgagtct gggtgttgta ttaattctga atctcatttt
      601 tctgatggga cgtcatgcca ctattcgggt aatgggattt ttggtattcc cattgattgc
      661 ctatttctta tttctttcca tttaccttgt cggtagttgg caacctgatc tattaacaac
      721 ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt
      781 tttcgccttt agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata
      841 tggcgaacac gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg
      901 cataagtgta ctgttctttg tctttagctg cctgctttct attccatctt cgtatattga
      961 agcggctaaa gaagaagggg tcaccatttt atcggcgctt tctatgctgc cgaacgcccc
     1021 agcatggttg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct
     1081 gggtacgtac tttggcgtta ttgaaggtgc cacagaggtc gtcaaaacaa cactacagca
     1141 ggttggtgta aagaaaagtc gtgcatttaa ccacgcacta tcaattatgt tggtatcgct
     1201 gattaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt acgcgatcag
     1261 cggcccgctc attgccatga tacttttcat catgcctacg ctgtcaacgt atctcatccc
     1321 ggcgcttaaa ccctggcgtt ccatcggaaa tctgattacg ctgatcgtgg gtatcctgtg
     1381 cgtatcggta atgttcttta gctaatatcc gttgtggatg gttcaaaatt gctacaattt
     1441 tgaaccattc catttttcat aatattcatt tgttattatt ccgccctatc acttctctcg
     1501 tcatatccta gaattatcag ttagttaaac acacaaagag atctgaacat cttaaataag
     1561 actatttaag atgcataact tagattcgca agatatcttc tggtcattca aaaacaattt
     1621 gcgatataga tattctcata ccatgagata gttatctgga ccacgttcga ccatgcaaga
     1681 ttaaatcagt gaaacataat catatttaat aacgagaaat gcatttttaa atatcttaat
     1741 cagaattata gcaaatacag attaatcgca atttttccca gcaaaattac aaaatcaagg
     1801 tgtattgaca tcatcaatca ccaa
//
LOCUS       DAZXUK010000443         1812 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZXUK010000443 DAZXUK010000000
VERSION     DAZXUK010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.446"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..96
                     /locus_tag="ACH3L8_004286"
     CDS             <1..96
                     /locus_tag="ACH3L8_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087123.1"
                     /translation="YEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            302..1585
                     /gene="gltA"
                     /locus_tag="ACH3L8_004287"
     CDS             302..1585
                     /gene="gltA"
                     /locus_tag="ACH3L8_004287"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFT4087124.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          493 a          432 c          403 g          484 t
ORIGIN      
        1 tatgaagtcg gtatttcacc taagattaac ttatgtaaca gtgtggaagt attgaccaat
       61 tcattcggga cagttattag tggtagacaa gtttaataat tcggattgct aagtacttga
      121 ttcgccattt attcgtcatc aatggattct ttacctgcaa gcgcccagag ctctgtaccc
      181 aggttttccc ctctttcaca gagcggcgag ccaaataaca aacgggtaaa gccaggttga
      241 tgtgcgaagg caaatttaag ttccggcagt cttacgcaat aaggcgctaa ggagacctta
      301 aatggctgat acaaaagcaa aactcaacct caacggggac acagctgttg aactggatgt
      361 gctgaaaggc acgctgggtc aagatgttat tgatatccgt actctcggtt caaaaggtgt
      421 gttcaccttt gacccaggct tcacttcaac cgcatcctgc gaatctaaaa ttacttttat
      481 tgatggtgat gaaggtattt tgctgcaccg cggtttcccg atcgatcagc tggcgaccga
      541 ttctaactac ctagaagttt gttacatcct gctgaatggt gaaaaaccga ctcaggaaca
      601 gtatgacgaa tttaaaacta cggtgacccg tcataccatg atccacgagc agattacccg
      661 tctgttccac gctttccgtc gcgactcaca tccaatggca gtcatgtgtg gtattaccgg
      721 cgcgctggcg gcgttctatc acgactcgct ggatgttaac aatcctcgtc atcgtgaaat
      781 tgccgcgttc cgcctgctgt cgaaaatgcc gaccatggcc gcgatgtgtt acaagtattc
      841 cattggtcag ccatttgttt atccgcgcaa cgatctctcc tatgccggta acttcctgaa
      901 tatgatgttc tccacgccgt gcgaaccgta tgaagttaat ccgattctgg aacgtgctat
      961 ggaccgtatt ctgatcctgc acgctgacca tgaacagaac gcctctacct ccaccgtgcg
     1021 taccgctggc tcttcggggg cgaacccgtt tgcctgtatc gcagcaggta ttgcttcact
     1081 gtggggacct gcgcacggtg gtgctaacga agcggcgctg aaaatgctgg aagaaattag
     1141 ctccgttaaa cacattccgg aatttgttcg tcgtgcgaaa gataaaaatg attctttccg
     1201 cctgatgggc ttcggtcacc gcgtgtacaa aaattacgac ccgcgcgcca ccgtaatgcg
     1261 tgaaacctgc catgaagttc tgaaagagct gggcaccaaa gatgacctgc tggaagtggc
     1321 tatggagctg gaaaacatcg cgctgaacga cccgtacttt atcgagaaga aactgtaccc
     1381 gaacgtcgat ttctactctg gtatcatcct gaaagcgatg ggtattccgt cttccatgtt
     1441 caccgtcatt ttcgcaatgg cacgtaccgt tggctggatc gcccactgga gcgaaatgca
     1501 cagttacggt atgaagattg cccgtccgcg tcagctgtat acaggatatg aaaaacgcga
     1561 ctttaaaagc gatatcaagc gttaatggtt gattgctaag ttgtaaatat tttaacccgc
     1621 cgttcaaatg gcgggttaat ttttatatgc ctaaacacaa aaaattgtaa aaataaaatc
     1681 cactatcgga cattcatata ttctaaaaaa aaatacatcg gtataaatta taaacaaata
     1741 gatgattttt aatcatctat cttcctctca gttacatgaa taaaccacca aatatagcgc
     1801 acccctataa tt
//
LOCUS       DAZXUK010000444         1809 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZXUK010000444 DAZXUK010000000
VERSION     DAZXUK010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.447"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            173..1198
                     /locus_tag="ACH3L8_004288"
     CDS             173..1198
                     /locus_tag="ACH3L8_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFT4087125.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="ACH3L8_004289"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="ACH3L8_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAZXUK010000445         1806 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZXUK010000445 DAZXUK010000000
VERSION     DAZXUK010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.448"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(79..1491)
                     /gene="pykF"
                     /locus_tag="ACH3L8_004290"
     CDS             complement(79..1491)
                     /gene="pykF"
                     /locus_tag="ACH3L8_004290"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFT4087126.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1499..1693)
                     /locus_tag="ACH3L8_004291"
     CDS             complement(1499..1693)
                     /locus_tag="ACH3L8_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087127.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT          448 a          426 c          443 g          489 t
ORIGIN      
        1 ttgcttctgg ttatcgatta aataaaaaaa gcgcccatca gggcgcttcg atatacaaat
       61 taattcacaa aagcaatatt acaggacgtg aacagatgcg gtgttagtag tgccgctcgg
      121 taccagtgca ccagaaacca taactacgac gtcacctttg tgtgccagac cgctctgcag
      181 agccagttct ttacccagac ggtagaaatc atcagtagaa gtgatctctt taaccagctg
      241 cggcacaacg cctttgctca gtaccaactg atgagccgtt ttttcgttgg tggtcagtgc
      301 caggatggtg gcatccggga agtatttacg tactgcgcga gcagatttac cgccctgggt
      361 agcaaccacg atcagcggag catccagttt ttcagcagtt tcaacggcac cacggcatac
      421 cgcttcggtg atgcgcagtt tacggttgtc attgttgaac tcgagacggc tgttcatcac
      481 gcggtcggta cgttcgcaga tggtcgccat gatagaaacc gcttccagcg ggtatttacc
      541 ttttgcggat tcaccagaca gcatcactgc gtcagtaccg tcgaggatgg cgtttgcaac
      601 gtcaccggct tctgcgcgag tcgggcgtgg gtttttgatc atggaatcca gcatctgggt
      661 cgcagtgata acgactttac gtgcacggat acatttttcg atcatcatct tctgggcgaa
      721 gataacttct tctaccggga tttctacacc caggtcgcca cgcgcaacca tgatgccgtc
      781 agaggcttcg aggatttcgt cgaagttgtt gaggccttcc tggttttcga ttttggagat
      841 gatgtggatg ttttcgccgc cgtgcgcttt caggtgctca cggatttcga taacgtcaga
      901 acgcttacga ataaaggaag cagcaacaaa gtctacgcct ttttcgcaac caaagatcag
      961 gtcctgtttg tctttttcag ccagtgctgg cagagcaatg gaaacgccag gcaggttcac
     1021 acctttgttt tcgcccaggt caccgttgtt cagcacttta cagataactt tgttaccttc
     1081 aatggcggta acttccatac cgatcagacc atcgtcaacc agtacggtgt tgccaacaga
     1141 caggtcagta gtgaaacctt catacgttac cgcaaccatt tcgctgttgc cgataacaga
     1201 tttatcagtg gtgaaagtaa aggtctgacc agctttcaga gaaacatcgt taccgccttc
     1261 cagtttcatg gtgcggattt ccggaccttt ggtatcaagc aggatagcgg cggttttacc
     1321 agttttgctc atcacgttgc gcagattctg aatgcgctga ccgtgttctg catagtcacc
     1381 atgagagaag ttcagacgca taacgttcat gccagcgtcc agcattttag ctaacatctc
     1441 ttcagattcg gttttcggtc cgatggtgca aacaattttg gtctttttca tgacagtctt
     1501 agtctttaag ttgagaagga tgggagaaac ttgctttctg ggcgcactgc cggaaagcga
     1561 atccggtatt acgaaagtgg tggtgcctcg ctctaaggat aggtgacatc gaaaaagcgt
     1621 gcagaggaat gtgtgcttgt gtctcagccc aacgtgagtg tctgtttttg agtcgttgag
     1681 ttctacagtc caaagtgctg aaaccattca agagtcaatt ggcgcgcatt atacgctgaa
     1741 attaatccaa aaggaaaaca aaaacagcgt ttcaaaaaat atacaacttg ttagacaata
     1801 aattgt
//
LOCUS       DAZXUK010000446         1804 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZXUK010000446 DAZXUK010000000
VERSION     DAZXUK010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.449"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..362)
                     /locus_tag="ACH3L8_004292"
     CDS             complement(<1..362)
                     /locus_tag="ACH3L8_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4087128.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACH3L8_004293"
     CDS             complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACH3L8_004293"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFT4087129.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          537 a          469 c          374 g          424 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 acca
//
LOCUS       DAZXUK010000447         1804 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZXUK010000447 DAZXUK010000000
VERSION     DAZXUK010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.450"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            103..1746
                     /gene="yojI"
                     /locus_tag="ACH3L8_004294"
     CDS             103..1746
                     /gene="yojI"
                     /locus_tag="ACH3L8_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFT4087130.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT          395 a          433 c          513 g          463 t
ORIGIN      
        1 ttccgtcagg caggtaaaaa agaataaaaa taagaattat tattgctgtg cgcgcgaaga
       61 tgtttaaact gcgggctgta attcattgtc cgggttttct gcatggaact tcttgtactt
      121 gtctggcggc agtatcgctg gccatttatc agtgtgatgg cgctaagcct cgccagtgcg
      181 gcattaggca ttggcttaat tgcttttatc aatcagcgcc ttatcgaaac ggcggatacc
      241 agtctgctgg tgttaccgga gtttctggga ttattgctgc tgttgatggc agtcactctc
      301 ggatcgcaac tggcgctcac cactttgggg catcacttcg tttaccgact gcgtagtgaa
      361 tttatcaagc ggattctgga tacccacgtc gagcgcattg aacaactcgg tagcgcctcg
      421 ttgctggcgg gattaaccag cgatgtgcgc aatatcacca ttgcttttgt gcgtctgccg
      481 gaactggggc aggggatcat tctcactatc ggttcatcgg cgtatctgtg gatgctgtcg
      541 ggcaaaatgt tgctggtaac ggctatctgg atggcgatca ccatctgggg cggttttgtg
      601 ctggtggcgc gggtgtacaa acatatggcg accctacgtg aaaccgaaga caagctgtac
      661 acggattttc aaactgtact tgaagggcgc aaagagctga ctctgaaccg ggaacgcgcc
      721 gagtatgtgt ttaacaacct ctacatcccc gatgcgcaag agtatcgcca tcatattatt
      781 cgcgcagaca ccttccatct tagtgccgtg aactggtcaa acatcatgat gctgggcgct
      841 atcggcctgg tgttctggat ggcgaacagt ctcggttggg ctgataccaa cgttgctgcg
      901 acctattcgt tgacgctgtt attcctgcgt acgccgctgc tttcggcggt tggcgcattg
      961 ccgacgctgc tgacggcgca ggtggcgttt aacaagctga acaaattcgc gctcgcgcct
     1021 ttcaaagctg agtttccgcg cccgcaggcg tttcccaact ggcaaacgct ggagctgcgt
     1081 aacgtgacgt ttgcttatca ggataacgcg ttttccgttg gtccgattaa tctcaccatc
     1141 aaacgtggcg agctgctgtt tctgattggc ggtaacggta gcggaaaatc gacgctggcg
     1201 atgttgttga cgggcttgta tcagccacaa agcggcgaaa tcttgctgga tggcaaacct
     1261 gtcagcggcg aacaaccgga agattatcgc aaactgtttt cggcagtgtt taccgatgtc
     1321 tggctgtttg atcaactgct ggggccggag gggcaacccg ctaacccgca actggttgag
     1381 aagtggctgg cgcagctgaa aatggctcat aagcttgagt taagcaacgg gcgtattgtt
     1441 aacctgaagt tatcaaaagg gcagaaaaaa cgcgtggcgc tgttgctggc gctggcagaa
     1501 gaacgcgata ttatcctgct ggatgaatgg gcggtggatc aggatccaca cttccgtcgt
     1561 gagttttatc aggtgttgct gccgctgatg caggagatgg gtaaaactat tttcgctatc
     1621 agtcatgatg atcattactt tatccacgcc gaccgcctgc tggaaatgcg caatgggcaa
     1681 cttagcgagc tgacgggcga agagcgcgat gccgcttcgc gtgatgccgt tgcccggacg
     1741 gcataatatt acgacgctga cctggcggac tgctgccgtc aggtcaatat gctaaaaatc
     1801 cctt
//
LOCUS       DAZXUK010000448         1766 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZXUK010000448 DAZXUK010000000
VERSION     DAZXUK010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.451"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(101..1312)
                     /locus_tag="ACH3L8_004295"
     CDS             complement(101..1312)
                     /locus_tag="ACH3L8_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4087131.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            complement(1584..1678)
                     /locus_tag="ACH3L8_004296"
     tRNA            complement(1584..1678)
                     /locus_tag="ACH3L8_004296"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1642..1644),aa:Sec,seq:tca)
BASE COUNT          466 a          397 c          392 g          511 t
ORIGIN      
        1 agacgcaaaa aaataatata ataacaaaaa tcctccagcc ttgtaggcca gaggataaaa
       61 tacttaattg aggaaaacat cagggaaaag tatttctgac ttaactttcc cggctggaga
      121 gtgaatcaat gtaatcagcg taccattgca tcatttcacg gcggccatcg agatacaggg
      181 catgattata ggtacccctg atggaatttt tatccacatg agctaactgc atttcaatcc
      241 aggcggaatt aaagccttgt tcatgcagaa ttgtactcat ggtgtgtctg aaaccgtgcc
      301 ctgtgagtct tcccttatat ccaagtaatt caattacttg gttaatgctt tccttactaa
      361 ttggtttgcg gggatcattt ctaccgataa agactagggg atgatgtttt gagattggtt
      421 caagctgctt aaatagcata atgacctgct cagagagcgg cacaatatga ggtcgcttca
      481 tcttcattac ttctgccggg atctcccaca attttgtctc aaaatcgata tcttcccagc
      541 gtgcaaaacg cagttcttgt gttcgtacgc cggttaacat aatgatctga gtagctgtct
      601 tagtgattat acttccggta taacccgcca gatcgttgag gaagtgggga agttcattag
      661 cagtaagaaa gggaaaatgt acttttttcg gtgtagctaa agcactggca agatcggggg
      721 ctgggttata gtctgcccgg ccagtaacaa ttgcatagcg gaacacctca ccacagcgct
      781 ggcggacctt gcgcattttc tccagagctc cacgtttttc cattttacgc agtgcttcca
      841 gtaacttcat tggtttgatt tcggcaatag ggcgcttgcc aatgtaggga aaaatgtctt
      901 tttcaaaggt atcaatgatt tcatcacgat agcgtaatga ccaccgatca gcttttaact
      961 ggtgccattc gcgagcaata gcctcgaacg tatttttcag actcatttgc tgcgcgattt
     1021 tttcttcctt ttttgcctcc cctggatcgc ctccagcagc gaggagtttc ctggcttcac
     1081 tgcgtttctc gcgagcatcg gctagcgtta catcggggta tacgccaata gaaagcatct
     1141 tttctttacc agcaaagcgg tacttcatgc gccagtattt agaaccatta gtgttaatca
     1201 gtagatacat accgccgcca tcagcaagct tatagggttt gtctttgggt tttgcggttt
     1261 tcactttgat atcgttaatg ccatgacatc ctccggcttt tgatgaattc atgttcctga
     1321 gggggatttc tgccagtaac taacctgata cccccaatac accccttgta taacaaagat
     1381 attgttaaaa gccgttgtta tgtatcacat ttttcctatt atgggggtgc ttgtatttca
     1441 ataggaaata acacccccta aagtaccccc aaaagaaact tgatacgggg tgaagtaggt
     1501 tgacctcagg atacaaataa atactgatat acaagggatt agaagagatt tattgacatt
     1561 gggagagttc agaagaggta aactggcgga agatcacagg agtcgaacct gcccgggacc
     1621 gctggcggcc ccaactggat ttgaagtcca gccgcctcac cggagacgac gatcttccgc
     1681 gcctcgattg ctacatggag gcggggcgca ttatagctac ttccttgagt ttctacatcc
     1741 cccagatcag ttttttccgc ctaata
//
LOCUS       DAZXUK010000449         1762 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZXUK010000449 DAZXUK010000000
VERSION     DAZXUK010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.452"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1729
                     /locus_tag="ACH3L8_004297"
                     /pseudo
     CDS             1..1729
                     /locus_tag="ACH3L8_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          677 a          300 c          342 g          443 t
ORIGIN      
        1 aactttaaaa ccaactgcta aacgtctggc aatttatttt attcaatctc ctttacccta
       61 tgacgaggag aaaaaacaat ggaaagtcac cggatggtat atctcatcaa ctgataatca
      121 gtcttcaact aaacagattg catttgaagg atatacagac acagataacc tctccgattc
      181 taaatttatt tcttcattta aatcctggtt tgaaagtgga aagataaaaa acattgctaa
      241 ctataatcca aaaggagaaa gagatggaaa atacgaaagt tattacaata gcaatggtaa
      301 cgtctattaa tctctaagtt tttctaatgg tcttatcaac ggtgagttta ttacatatta
      361 tgaaaatggg gccattgaaa gtaaacgtca ttttaataac gacaaaatta aagatggtga
      421 atgccctcat ttttatgaag atgggaaaat caaacagcaa catagctatc tgaatagtaa
      481 actcgatggt cctgcatatg aatattatcc tgatggcaaa ctcatgcagg aaaatttcta
      541 tcagcaaagt gtgttgattg gaaacgatac cagttactac caatccggaa aaataagctc
      601 tatacacaat agaaattccc gtggtcagta tgatggagct aacgaacgtt atagtgaaga
      661 gggtaagtta ctgtctaaat ccgtctataa agacggaaaa caaatttctg ttcaaacctg
      721 gtatgaaaat gggcagaaag aagaggagaa acattttgat gagcaaggcc aattaaatgg
      781 tctggttaag caatggtata aaaatggtaa tcttgcaaaa tcacaaaact ataaacatga
      841 catactcgat ggtaacagtg aagagtggta cgaaaatggc atccctgaaa gtttgtaccc
      901 ttataagaat ggaaaaaccg acggcattgc caaatcatgg aataagtatg gtaaattaac
      961 ctattcaatc gaatacaaaa atggcgtaga gaatggggca tatcgtaact ggagcaaaaa
     1021 cacaggtaaa ctaaccaaag aaaccctgta cgtcaatggt atcagacagg gagtggaaaa
     1081 agagtttaac gatcgcacag gtaaactatt aacctccacg caatatgtta ataataaaag
     1141 acatggtacg gaggaaactt atgatcaaaa cggaataaaa tatattacat gctatcaaaa
     1201 tgatcagaaa ttatcgtctt tagacaatcc aacacaaata aaagataatg caacaacagg
     1261 cgattccagt gctcaatttg cattagggaa atacgaattc atctgtgcaa atatagatga
     1321 agggattaaa tggttaacca aatctgcaga gcaaaaaaac actgatgcga tatacttttt
     1381 agctacagcc tacaaaggca atggtatacc tgcaaacaat gaaaaatacc tagcatatct
     1441 gcaacaagct gcaacactgg gcagtagtaa tgcccaggcg gaaattggct atttatatct
     1501 catcggaaaa gaactaccac agaatcttcc tgatgctggt gtctggttca aaaaagcagc
     1561 tgcacaaggt aattttgccg cacatttcta tctgggaagg atgtatcaga atggtgacgg
     1621 cgttgagaga aatatggaaa aagccaggtt tcatctatct aatgcggcag aaggtggaat
     1681 agaacctgcg cttaaagcat taaatgaact tgaacatcaa acaaaataaa gcgctcaggg
     1741 accacgtaag gttcatcaaa ca
//
LOCUS       DAZXUK010000450         1747 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZXUK010000450 DAZXUK010000000
VERSION     DAZXUK010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.453"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            454..1302
                     /locus_tag="ACH3L8_004298"
     CDS             454..1302
                     /locus_tag="ACH3L8_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFT4087132.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1319..1600
                     /locus_tag="ACH3L8_004299"
                     /pseudo
     CDS             1319..1600
                     /locus_tag="ACH3L8_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
     gene            1636..>1747
                     /locus_tag="ACH3L8_004300"
     CDS             1636..>1747
                     /locus_tag="ACH3L8_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087133.1"
                     /translation="MAGIRSTLIKEKNMNTNYEATVATTDNIVHEVYLEGK"
BASE COUNT          499 a          398 c          411 g          439 t
ORIGIN      
        1 agcattatat agttctactc tggcgagata cgttggcata ttcatttcct tactggttgt
       61 gtgagaactt cagtaaagat accaccaaag cccggaagtg gtgaaataaa accgggcaca
      121 acacgaaggc gcatttccgg tatccataaa gagtcggtct tgtctgtgaa atttaaatgg
      181 tgggagtgcg cttccggttg taaataacga cattgctgtg tgaagtacca gttggcggca
      241 tcggtttaat tgctggctga tgtccgccct ttttaaagtg aattttgtga tgcggtgaat
      301 gcggctatgc gcacgcggca cagttaaaag tcatgttagt ccttattggt ttgggtggga
      361 aagccaactg taattgttaa ctggttaacg tcacctggag gcaccaggca ccgcatcaac
      421 aaagttcact tcggtgatga aaggaaagag aaaatgttga atgtagctat tgaaaaccag
      481 aacgggtgga attatagtgc acctgcacct cataaaacgg gtgccgggat tgctaccccg
      541 acgatgacta ctgcgcataa ccgcgctcag gcggtttttt tatgcgtcaa gcacagccac
      601 attcagatta tggtggggcg tgcagggcag ccgcaaggct ggccggtttc ggtagtcacc
      661 ggttgtagca accctgtgcg tctcaccacc catgagattg ctacctctgg tggtgagtca
      721 tttaaactga ctatcgaggc tgccatcatg gctactatcc ttaccctttc tcaccctgac
      781 gcaaccatcg aaaatggtcg cgctgtcact acgtctgttg cggttgcgga gttcttccgg
      841 aaaatgcaca aaaacgttat tcagaagatc gaaactctcg aatgctcgcc ggagtttaac
      901 cggcttaatt ttaagcccgt cacctatacc gacgccaaag gcgaaaaacg cccaatgtac
      961 caaatcacca aaaacggctt cgttttcctg gtgatgggct tcactggcaa aaaagccgcc
     1021 gcattcaagg aagcctacat tgctgagttc gaccgcatgg aggccgaact gcgccagaat
     1081 aacaccccac ctgcagacaa gatgattccg ggtgatggtc gcactctggt tgttcacttc
     1141 gacaaattcg gcaatgtcga attcaccgaa accgttcctg atggcgcact ggtctgtacc
     1201 ctggagactt tccggtttta tctggaaaaa cagggatgga ctcttgttaa ccggggcgca
     1261 attaaaaata tgactgtgga acaattactt tctttaaaat aaaaatggag ctacggatat
     1321 gaaccctatg cttgattatt gttttacctc tgcacctcag cagaccataa cacacaccgg
     1381 aatcaaaaat ttgttatgga taatcaaaaa aaacctccag tagaaaggtg atcccgattt
     1441 aattccctgt gcttatggcc tgtgtgacag acaagtaagc cctgataagg cttacactgc
     1501 tgaacttatt tacatgcacg gaaccatgat gaggaaaaag aaacgcaaat attgtagtga
     1561 aatctgcgcc gaaaaagacc agatggcaca cgaactttaa ttaactgact attcgaaact
     1621 gaatttatgc cagcaatggc agggattcgc tcaaccttaa ttaaggagaa aaacatgaac
     1681 accaattatg aagccactgt tgcaactact gataacattg ttcacgaggt ttacctggaa
     1741 ggaaagc
//
LOCUS       DAZXUK010000451         1746 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZXUK010000451 DAZXUK010000000
VERSION     DAZXUK010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.454"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            311..505
                     /locus_tag="ACH3L8_004301"
     CDS             311..505
                     /locus_tag="ACH3L8_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087134.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            866..994
                     /locus_tag="ACH3L8_004302"
     CDS             866..994
                     /locus_tag="ACH3L8_004302"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087135.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(1304..1640)
                     /locus_tag="ACH3L8_004303"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="ACH3L8_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          521 a          388 c          374 g          463 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctga
//
LOCUS       DAZXUK010000452         1733 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZXUK010000452 DAZXUK010000000
VERSION     DAZXUK010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.455"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(9..215)
                     /locus_tag="ACH3L8_004304"
                     /pseudo
     CDS             complement(9..215)
                     /locus_tag="ACH3L8_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(268..519)
                     /locus_tag="ACH3L8_004305"
     CDS             complement(268..519)
                     /locus_tag="ACH3L8_004305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087136.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGTFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
     gene            971..1255
                     /locus_tag="ACH3L8_004306"
     CDS             971..1255
                     /locus_tag="ACH3L8_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFT4087137.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            1255..1530
                     /locus_tag="ACH3L8_004307"
     CDS             1255..1530
                     /locus_tag="ACH3L8_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFT4087138.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          476 a          435 c          407 g          415 t
ORIGIN      
        1 ctcactcctt atttacccgg gaaatacttc tccagcaatg catacaggat ggcagcggtt
       61 tcagcatcca ttatgccgtc ataatttttc tgccggaaat gaagctgaaa agcgcggaca
      121 agcgccttat atccgtcatc cgtctccaca ccagaaatac tgtatccata ggtcttcagc
      181 cgtgacacga tatcaacctt atccggaatg ccctgcgatt caaaaaataa atatttagtc
      241 agtaatttta tgtttttcct gaaaaaatta ctctttttcg aactccagtg agcgttcacg
      301 actcatcagt gtgaaaatga tggcaaaaac agcagacagt atcagtacga taaatgtggc
      361 cccccatccc agataatcga caacaagtcc cattcccagt gtcgcaacaa ttgagccgaa
      421 gatataggaa aatgtgcctg acagccctaa cagaataagt ttacgcgtaa atcgccctga
      481 atccagaaga gcattaagta tcacataaac aggttccata ccagtcctga ccgcatcagg
      541 acgccagtaa ataaaatctc gtagtccacc ggcatcagag acaagagtaa tcgtcgttac
      601 cggtatttcg actggtttgt actggtgtga gcggacaaaa ctactgtacg tctctcatac
      661 acgctcgtaa gcggccctca cggcccgcta acgcggagat acgccccgac tgcgggtaaa
      721 cccttgtcgg gaccactccg accgcgcaca gaggcatttt catcgctgac aggccgcatg
      781 gcctcgcaga gccggagggg gactggtgat attcggacga ccgtaccggc ttgcgccggg
      841 cttcggcggt tttacacctg tgctacatgt aaccaccgta tgccgcctta tatgcgctga
      901 ctcgcattat ttgccttgtt ccgtctgaaa gtaatcacta tgattaaata tgattaacaa
      961 ctaatcgggt atgcaaatga aaaacaatac cgcacaagca acaaaagtaa ttaccgcgca
     1021 tgtgccatta cctatggctg ataaagtcga ccagatggcc gccagactgg aacgctcccg
     1081 gggctgggtt atcaaacagg cgctttctgc atggcttgcc caagaggagg agcgtaatcg
     1141 cctgacgctg gaagccctgg acgatgtgac atccggacag gttatcgacc atcaggctgt
     1201 acagtcctgg gcggacagcc tcagtactga ccatctgtta ccggtgccac gctgatggaa
     1261 ctgaagtgga ccagtaaggc actttctgat ttggcgcggt tatatgattt tctggtgctg
     1321 gccagtaaac ctgctgccgc cagaacggtg cagtccctga cacaggcgcc ggtcattctg
     1381 ttaactcatc cacgtatggg agaacagttg tttcagtttg aacccaggga ggtcagacgg
     1441 atttttactg gcgagtacga aatccgttac gaacttactg gccagactat ttatgtattg
     1501 cgcctgtggc acacacgaga gaacaggtag attgggtgtg gaggcaactt taacatagcc
     1561 tgcaggtatt aattctggag cattgttatc aggccagata ttttgcattc cattaaataa
     1621 tcttgtagga gataaaataa attacgatga aataacgaaa attaccgctc gtgactggcg
     1681 gcaatatcgc gctccgggct ggcaaataac ccatcagaaa cgttattgcc aga
//
LOCUS       DAZXUK010000453         1733 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZXUK010000453 DAZXUK010000000
VERSION     DAZXUK010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.456"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            119..813
                     /locus_tag="ACH3L8_004308"
     CDS             join(119..365,365..813)
                     /locus_tag="ACH3L8_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFT4087139.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(932..>1733)
                     /gene="murI"
                     /locus_tag="ACH3L8_004309"
     CDS             complement(932..>1733)
                     /gene="murI"
                     /locus_tag="ACH3L8_004309"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFT4087140.1"
                     /translation="AESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPYGNR
                     SADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSIIEPA
                     VEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGNLNA
                     PAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAISIK
                     NHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
BASE COUNT          488 a          355 c          368 g          522 t
ORIGIN      
        1 tgattgtaag ttgtgattat tataatttag ttattgttag tatgttttat aatgctggat
       61 gggtgatgcc tctaattagt tgaatctgat gtataatacg ggcttttgag gttatctcat
      121 gaccagcgtt aatattcatt gtccccgttg tcagtcagct caggtttacc gccatggtca
      181 gaaccctaaa ggccgtgaca gacttcgctg ccgtgactgc caccgtgtgt ttcagttcac
      241 ttatacttat caagcacgta agccgggtat gaaagagctg ataactgaaa tggcctttaa
      301 tggtgccggg gttcgcgata ccgccaggac actgaaaatt ggtagtaaca ccgtcatccg
      361 gactttaaaa aactcgcgcc aaagcgaata acttcttcgc ctgttgccca tgctgatgtg
      421 gcgtttatct gcgagcttga tgagcaatgg agctacgttg gcagtaaagc ccggcaacat
      481 tggctcgggt acgcgtacaa caccaaaaca ggcggtgtac tggcctacac ttttggtccc
      541 cgaaccgatc aaacgtgccg ggagctactg gcactgctta cacccttcaa catcggcatg
      601 ctgaccagcg atgactgggg cagctatggc cgggaggtgc cgaagaataa gcatctgacc
      661 ggcaaaatat tcacccaacg cattgagcgt aataatctga cgctacgcac ccgcattaag
      721 cggttgggtc gtaaaacaat ctgcttctca cgctcagttg agatccacga aaaagtgatc
      781 ggagcgttta tcgaaaaaca catgttctac taattggatg cactacccag tatgtaactg
      841 ggtttgtcca taaaatgcac cttactcagt tggtgtccaa cttttggggt gcagttcaga
      901 aggacgggat tttacggtgt accggataaa tttaaaactg cctgatactg aatgtatgag
      961 gtttcgtaat gctcaattcc cctagtaccg ttttgtagat ctccatactt ccactagtat
     1021 ttatttgcaa agttcctata tttgaggagt ttaataaatt tagctgtccc aagtgatttt
     1081 taatagagat agcctgatca tgtgcaggat taataaattt tatatccggt gcaactgctt
     1141 caaaaaaatt ctgaacaatt ggatagtgag tacagccgag aataatttca tttactggat
     1201 ggagggaaat taaatggtta acatgttttt tgatgtcatt caagattgca ggagcattaa
     1261 gattaccttc ctcaattaac attgcaagat ttttgcttgg ttctccaaat acatttattg
     1321 ttgaattcaa tttttttatt agctctttgt gacaccccgt tttaatagta aactcggtag
     1381 cgataatacc aacattttta tattgctggc gaatcacatc ttctactgca ggttcaatga
     1441 tggataagat aggaaacgga aagctcttag catatccgct gtattcttcg gattccaaag
     1501 ttgaggatat agtattacat gcgacagcaa ctagtttgac attccttgac tgtaaaaaag
     1561 tcagcatttt tttagtcaaa ctgatgatat catcggcaga acggttgcca taaggtgcat
     1621 tcatattatc tccacaatag ataatatctt caccaggaag caaattaatt atttccttta
     1681 ccgatgtcaa tccaccgaca cctgagtcta ttacaccaat tggagattca gca
//
LOCUS       DAZXUK010000454         1725 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZXUK010000454 DAZXUK010000000
VERSION     DAZXUK010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.457"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACH3L8_004310"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACH3L8_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFT4087141.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..>1725)
                     /gene="phoA"
                     /locus_tag="ACH3L8_004311"
     CDS             complement(446..>1725)
                     /gene="phoA"
                     /locus_tag="ACH3L8_004311"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFT4087142.1"
                     /translation="LTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGA
                     GGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHE
                     KDHPTILEMAKAAGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALE
                     KGGKGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAAS
                     LNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLA
                     QMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKK
                     EGNTLVIVTADHAHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQ
                     LRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK"
BASE COUNT          361 a          468 c          427 g          469 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca tcggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
     1561 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag
     1621 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
     1681 cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagc
//
LOCUS       DAZXUK010000455         1715 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZXUK010000455 DAZXUK010000000
VERSION     DAZXUK010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.458"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1140
                     /locus_tag="ACH3L8_004312"
     CDS             <1..1140
                     /locus_tag="ACH3L8_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HFT4087143.1"
                     /translation="HHGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHI
                     ANMWLSKVVDDEGKEIFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPE
                     LLSQALSGKTVDLKIVSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRN
                     SDGLLKEVSVNLKVVTFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIG
                     GWAAEAIEKNPPCKNDVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVP
                     CWNCKSGKDRTGMQDAEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEI
                     QEMNTGVPGNKVMKKLPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            1150..1512
                     /gene="ipgE"
                     /locus_tag="ACH3L8_004313"
     CDS             1150..1512
                     /gene="ipgE"
                     /locus_tag="ACH3L8_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFT4087144.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            1512..>1715
                     /gene="ipgF"
                     /locus_tag="ACH3L8_004314"
     CDS             1512..>1715
                     /gene="ipgF"
                     /locus_tag="ACH3L8_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFT4087145.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDF"
BASE COUNT          598 a          212 c          398 g          507 t
ORIGIN      
        1 catcatggga aaaattatgg ttttcaatta actcctgcct ctcatatgaa gatagggaat
       61 aaaaatatat ttgtcaaaga gtataatgga aaaggaattt gttgtgcttc tacgagagag
      121 agtgatcata tcgcgaatat gtggctttca aaagtggtgg atgatgaggg gaaggaaatt
      181 ttttcgggta tcagacatgg agttatatca gcctatggat tgaaaaaaaa ctcatctgaa
      241 agagctgttg ctgctcgtaa taaagctgag gagttagtaa gcgcagcatt atatagcaga
      301 ccagagttat tatcacaggc tttgtctggt aaaacagtag atttaaagat tgtttcgact
      361 tctcttctga cgccgaccag tttaaccggg ggagaggaga gtatgttaaa agatcaggtg
      421 aacgcattaa aagggttaaa ctctaaaagg gggaagccaa cgaaattatt aattcggaat
      481 agtgatggtc ttctgaaaga agtaagcgtt aatctaaaag tggtgacatt taactttggt
      541 gtaaatgaat tagcgctaaa aatgggctta ggctggagga atgttgacaa gcttaatgat
      601 gaatcaatat gttctttgtt gggggataat ttcctcaaaa atggtgtgat tggaggttgg
      661 gctgcagaag caatagaaaa aaatccacca tgtaagaatg atgtgatata tctggctaac
      721 cagataaaag agattgtaac caaaaagtta caaaaaaatg ataatggaga gccgtataaa
      781 ttgtcacaaa gaatgactct attggcttat actataggtg ctgtaccttg ctggaattgt
      841 aagagtggga aggacagaac aggtatgcag gatgctgaaa ttaaaagaga aataataaga
      901 aaacatgaaa caggtcagtt ttctcaatta aatagtaaat tatcctcaga agagaaaaga
      961 ttattttcta ctattctaat gaatagtggt aatatggaaa tccaagagat gaatactggt
     1021 gtgcccggaa ataaagtcat gaaaaaattg ccgctatctt cgttagagct atcttattct
     1081 gaaagaatag gggacccaaa aatatggaat atggtgaaag ggtattcgtc atttgtataa
     1141 gaggaatata tggaagattt agcagatgtt atttgccgag ccttgggtat ccctttaatt
     1201 gatatcgatg atcaagcaat catgcttgat gatgatgtgc taatatatat tgaaaaagaa
     1261 ggagattcaa taaatttatt atgccctttt tgtgcattgc ctgaaaatat aaatgatctt
     1321 atatatgcgt tgagcctaaa ttactcagaa aagatatgct tagctactga tgatgaaggc
     1381 ggaaatctaa ttgcgcgatt agatcttacc gggataaatg aattcgagga tgtatatgtt
     1441 aatacagaat attatatttc gcgtgtgcgt tggctgaagg atgaatttgc gcgaagaatg
     1501 aaggggtatt aatgtcccgt ttcgtattta ttttgctgtg ttttatccca tatttaggga
     1561 gagctgattg ttgggataag gctggtgaaa ggtacaatat tccatcaagt ttgttaaaag
     1621 cgattgcgga aaaagaatcc ggatttaata aatctgcagt caatgttaat aacaatggaa
     1681 gtaaagatta tggtataatg caaataaatg acttt
//
LOCUS       DAZXUK010000456         1660 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZXUK010000456 DAZXUK010000000
VERSION     DAZXUK010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.459"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            187..480
                     /gene="yicS"
                     /locus_tag="ACH3L8_004315"
     CDS             187..480
                     /gene="yicS"
                     /locus_tag="ACH3L8_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFT4087146.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..>1660)
                     /gene="nepI"
                     /locus_tag="ACH3L8_004316"
     CDS             complement(521..>1660)
                     /gene="nepI"
                     /locus_tag="ACH3L8_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFT4087147.1"
                     /translation="NWSAVFSVAFCVACLIIVEFLPVSLLTPMAQDLGISEGVAGQSV
                     TVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLVSFANSFSLLLIGRACLG
                     LALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIAAPLGSFLGELIGWRNVF
                     NAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGVMAGMIAIFMSFAGQFAF
                     FTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFILKRSVKLALAGAPLILAV
                     SALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSLADQAEKAGSIQVAVIQL
                     ANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVKMKKS"
BASE COUNT          531 a          444 c          375 g          310 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt
//
LOCUS       DAZXUK010000457         1641 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZXUK010000457 DAZXUK010000000
VERSION     DAZXUK010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.460"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            31..1554
                     /locus_tag="ACH3L8_004317"
     CDS             31..1554
                     /locus_tag="ACH3L8_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFT4087148.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          435 a          363 c          368 g          475 t
ORIGIN      
        1 tgtattaaac cctttggccg ggacaagcaa atgcgcaata cactcatacc catcctcgtt
       61 gcgatatgct tgtttattac cggtgtcgct attttgaaca tccagctctg gtattccgca
      121 aaagcagagt acctggcggg agcaagatat gccgccaaca atatcaatca tatacttgaa
      181 gaagcgtcac aggcgactca aacagcggtt aacattgccg ggaaggaatg cgacctcgag
      241 gagcaatatc agcttggcac tgaagcagct ctgaaacctc acctgcgcac aatcatcatt
      301 ctcaaacagg gaatagtctg gtgtacatcc ctgcctggga atcgggtcct gctgtctcgt
      361 attcctgttg tcccggacag taatttactg ttggctccgg caatcgacac cgttaataga
      421 ttacctatcc tgctctatca gagccaattt gcagatacgc gcattttggt tacgataagt
      481 gatcaacata ttcgcggggc gcttaatgta cccttgaaag gggtaaggta tgtattacgc
      541 gtggcggatg acattattgg accaacggct gatgtgatga cgcttaatgg acattatccc
      601 tataccgaga aggttcactc cacaaaatat catttcacta ttatctttaa cccgccgcca
      661 ctctttagct tctacagact tatcgataaa ggctttggga ttttaatatt tattctgtta
      721 atcgccagcg ccgctgcctt cctgcttgat agatatttca ataaaagcgc aacgcctgaa
      781 gagatcctgc gacgggcaat aaataatggg gagatcgtcc ctttttacca acctgtggta
      841 aatggtcggg aagggacatt gcggggagtt gaggtgttag cccgctggaa acaacctcac
      901 ggtggatata tttcacccgc ggcatttatt ccacttgctg aaaaatccgg attaatcgtt
      961 ccgcttacac aaagcctgat gaatcaggtt gccagacaga tgaacgctat cgcgagtaaa
     1021 ctgccggaag gttttcatat tggaattaat tttagcgctt cgcatattat ttcgccgacg
     1081 tttgtcgacg agtgtttaaa tttccgtgac agttttaccc gccgcgattt aaaccttgtt
     1141 ctggaagtca ccgagcgtga gccattgaat gttgatgaaa gtctggttca gcggttgaac
     1201 attctgcatg aaaatggttt tgtcatcgcg ctggatgatt tcggtactgg ctactcaggg
     1261 ctttcttatc ttcatgacct gcatattgat tatatcaaaa ttgatcacag tttcgttggc
     1321 cgcgtaaacg cagacccaga atcaacccga attctggatt gtgtattgga tctggcgcgt
     1381 aaactttcga tcagtatcgt cgctgaaggt gtcgaaacga aagaacaact tggctatctg
     1441 aaccaaaata atatcacatt tcagcagggt tattatttct ataaacctgt tacatacatc
     1501 gacctggtca aaattatcct ttctaaaccg aaggtgaagg ttgtggttga gtgaaaattg
     1561 atcagtaagg ccatagtgcg gtgtaattat agacagctaa ttagctcgtt gcctcttgtt
     1621 actattgttc attattttgt t
//
LOCUS       DAZXUK010000458         1628 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZXUK010000458 DAZXUK010000000
VERSION     DAZXUK010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.462"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(106..387)
                     /locus_tag="ACH3L8_004318"
                     /pseudo
     CDS             complement(106..387)
                     /locus_tag="ACH3L8_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            complement(534..1394)
                     /locus_tag="ACH3L8_004319"
     CDS             complement(534..1394)
                     /locus_tag="ACH3L8_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFT4087149.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          442 a          421 c          360 g          405 t
ORIGIN      
        1 tatcaagcga cttctcctat cccctgggaa cacatcaatc tcaccggaga atatcgctgg
       61 ccaaagcctt agcgtaggat tccgcccctt cccgcaaacg accccaaaca ggaaacgcag
      121 ctgaaacggg aagctcaaca cccactgacg catgggttgt tcaggcagta cttcatcaac
      181 cagcaaggcg gcactttcgg ccatccgccg cgccccacag ctcgggcaga aaccgcgacg
      241 cttacagctg aaagcgacca ggtgctcggc gtggcaagac tcgcagcgaa cccgtagaaa
      301 gccatgctcc agccgcccgc attggagaaa ttcttcaaat tcccgttgca catagcccgg
      361 caattccttt ccctgctctg ccataagggg tctgacgctc agtggaacga aaactcacgt
      421 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
      481 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
      541 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
      601 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
      661 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
      721 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
      781 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
      841 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
      901 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
      961 tccttcggtc ctccgatcgt tgtcagaagt aagttggcag cagtgttatc actcatggtt
     1021 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1081 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1141 ccggcgtcaa cacgggataa taccgcacca catagcagaa ctttaaaagt gctcatcatt
     1201 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     1261 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     1321 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacgaaaa
     1381 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttacca gggttattgt
     1441 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     1501 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     1561 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattttataa accgtggagc
     1621 gggcaata
//
LOCUS       DAZXUK010000459         1628 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZXUK010000459 DAZXUK010000000
VERSION     DAZXUK010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.463"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..239
                     /locus_tag="ACH3L8_004320"
     CDS             <1..239
                     /locus_tag="ACH3L8_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HFT4087150.1"
                     /translation="GVFISLVPMLLIFLKGYFLNSTKTSLMMSLILFFSITVSITNPF
                     LFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            243..617
                     /locus_tag="ACH3L8_004321"
     CDS             243..617
                     /locus_tag="ACH3L8_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFT4087151.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            680..1618
                     /locus_tag="ACH3L8_004322"
                     /pseudo
     CDS             680..1618
                     /locus_tag="ACH3L8_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT          489 a          230 c          303 g          606 t
ORIGIN      
        1 ttggtgtatt tatatcatta gttcctatgt tgcttatctt tttgaaaggc tattttttaa
       61 atagtacaaa aacatcatta atgatgtcgt taatactttt tttcagtatt accgtttcta
      121 taactaatcc atttcttttt acacccatgg gaatttttat tataggcgtt gtagttttat
      181 gggtattttc tatagaaaat atccaaatta gtaataacct cacttctgga gcaaaataaa
      241 ggatgttaaa aatagggaag ttattgacca gttctttttt tagttatttt ttaattggaa
      301 ttgtcaatac tgctttacat tggggggtat tttatgcgtg ttataataat ttagcttttg
      361 gtcaaggccg aagtaacatt gttggtttta tatgtgcagc aaccttttct ttttttgcaa
      421 atgcaagatg ctcatttaaa gtatctgcaa ccaaggctag atattttata tttatatttt
      481 ttatgggggc gatgagttat ttattcggcg ttttatttga tctcttagct ttatctccaa
      541 tatttacatt gttcaccttt tctcttttta gtttggtttt aggatattgc gcatcaaaat
      601 atttcatttt tcgttagtaa acgatttgtt aaaaacacaa ttacttgcac attagctaaa
      661 tagtttatat gaggggctta tgtatcactc ttttaatatc gctgtaatta ttccttgtta
      721 taatgagcag aaggcaatag caaaagtcat aaatgatttc aaaacgaata ttccaactgc
      781 ttctatatat gtatttgata ataattcgac tgacagtaca gcacaagtgg cagaggatgc
      841 tggagcaaca gttcactctg ttcctctgaa aggcaagggg aatgtcgtaa gacgtatgtt
      901 ctcagatgta gatgcggata tttatctaat ggttgatgga gatgacactt atgatgcaag
      961 ttctgctcca gaaatgatta accatctcat aaaaaatcaa ttggatatgg ttgtaggatg
     1021 tcgtcaagag aatggagatc aaaatacata cagaaaaggg catcgctgag gaaataaatt
     1081 gttgactggt actgttcaga ctatctttaa agggaatttt actgatatgt tgtcggggta
     1141 tcgtgtattt tccagacgct atgttaaatc ttttccatgt ctatcaaggg gatttgaaat
     1201 cgaaactgag cttacaattc atgctttaga attacgtatg aagtatggtg aggttaatac
     1261 aaaatatggc gagcgatctg aagggagtgt gagcaaacta tcaacttgga gtgatgggtt
     1321 caagattctg aaaacaataa ttaaacttta ctcgcttgaa cggccactat acttttttag
     1381 tattatcggc gttttacttg ctgctttgtc tataatttta ggattgccca tcattgttga
     1441 ttatattgat acaggtttgg tgagaagatt tccgactgcc tttttaactg cctccatcat
     1501 gttatcgtca ataatggcat ttgtatgtgg cattattttg cattcaaata caacaactag
     1561 acgtgaaatg aaggcattat tttatctttc tgaaaaaaac tataaactga taatgtaaat
     1621 agagtatt
//
LOCUS       DAZXUK010000460         1613 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZXUK010000460 DAZXUK010000000
VERSION     DAZXUK010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.464"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            71..>1613
                     /locus_tag="ACH3L8_004323"
     CDS             71..>1613
                     /locus_tag="ACH3L8_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HFT4087152.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSLRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKLLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPAAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTDITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWEALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWLFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLL"
BASE COUNT          395 a          444 c          471 g          303 t
ORIGIN      
        1 atcaactgga agcacccgaa acgaacggaa cgcgctggaa tccgcatata acccgttgta
       61 aagtgaggat atggacacct cacttgctca tgagaatgcc cgcctgcggg cactgttgca
      121 gacgcaacag gacaccatcc gccagatggc cgaatataac cgcctgctct cactgcgggt
      181 ggcggcttat gcttccgaaa tcaaccggct gaaggcgctg gttgcaaaac tgcagcgcat
      241 gcagttcggt aaaagctcag aaaaacttcg cgcaaaaacc gaacgacaga tacaggaagc
      301 acaggagcga atcagcgcac ttcaggaaga aatggcggaa acgctgggtg agcaatatga
      361 cccggtactg ccatccgccc tgcgccagtc ttcagcccgt aaactgttac cggcctcact
      421 tccccgtgaa acccgggtca tccggccgga agaggaatgc tgtcctgcct gtggtggtga
      481 actcagttct ctgggatgtg atgtgtcaga gcaactggag cttatcagca gcgcctttaa
      541 ggttatcgaa acacaacgtc cgaaactggc ctgttgccgg tgcgaccata tcgtgcaggc
      601 accagtacct tcaaaaccca ttgcacgcag ttatgccgga gcggggcttc ttgcccatgt
      661 tgtcaccggg aaatatgcag accatctgcc gttataccgc cagtcagaaa tataccgtcg
      721 tcagggcgtg gagctgagcc gtgccacact ggggcgctgg acaggtgctg ttgctgaact
      781 gctggagcca ctgtatgacg tcctgcgcca gtatgtgctg atgcccggta aagtccatgc
      841 cgatgatatc cccgtcccgg tccaggagcc gggcagcggt aaaacccgga cagcccggct
      901 gtgggtctac gtccgtgatg accgtaacgc cggttcacag atgcctgcgg cggtctggtt
      961 cgcgtacagc ccggaccgga aaggcatcca tccacaaaat cacctggccg gttacagcgg
     1021 agtgcttcag gccgatgctt acggtggcta ccgggcgtta tacgaatccg gcagaataac
     1081 ggaagccgcg tgtatggccc atgcccggag aaaaatccac gatgtgcatg caagagcgcc
     1141 aaccgatatc accacggaag ccctgcagcg tatcggtgaa ctgtatgcca tcgaggcaga
     1201 agtccgggga tgttcagcag aacagcgtct ggcagcaaga aaagccagag ctgcgccact
     1261 gatgcagtca ctgtatgact ggatacagac tcagatgaaa acactgtcgc gtcactcgga
     1321 tacggcaaaa gcgttcgcat acctgctgaa acagtgggag gccctgaacg tgtactgcag
     1381 taatggctgg gtggaaatcg acaacaacat cgcagagaac gccttacggg gagtggccgt
     1441 aggccggaaa aactggctgt tcgcgggttc cgacagcggt ggtgaacatg cggcggtgtt
     1501 gtactcgctg atcggcacat gccgtctgaa caatgtggag ccagaaaagt ggctgcgtta
     1561 cgtcattgaa catatccagg actggccggc aaaccgggta cgcgatctgt tgc
//
LOCUS       DAZXUK010000461         1602 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZXUK010000461 DAZXUK010000000
VERSION     DAZXUK010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.465"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(334..480)
                     /locus_tag="ACH3L8_004324"
                     /pseudo
     CDS             complement(334..480)
                     /locus_tag="ACH3L8_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            534..716
                     /locus_tag="ACH3L8_004325"
     CDS             534..716
                     /locus_tag="ACH3L8_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFT4087153.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(817..1146)
                     /locus_tag="ACH3L8_004326"
     CDS             complement(817..1146)
                     /locus_tag="ACH3L8_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFT4087154.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1318..>1602)
                     /locus_tag="ACH3L8_004327"
     CDS             complement(1318..>1602)
                     /locus_tag="ACH3L8_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087155.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          435 a          368 c          377 g          422 t
ORIGIN      
        1 tagcaatgtc tgtcgcacaa caaccacaat gtcacaaata aaaaatgaga ataaccagtt
       61 ccctgaaggc gacgtagccc ggtacaagag taacccacgt tagcgcttgg cgacggcgga
      121 acaaagctga ttcgcgacgt aagaaagtgg caggctcata tgacggacct ctgtaatgcg
      181 gtatccaacc cgcgaatatc agcatgattc accgtcggat ttactgctac gtcgccctca
      241 gggaattaaa cctaaaaaga aaatcgtcaa aattagagat tccggtttga cacggaaagt
      301 catatcagcg tcaacggagc accgtattga cgcttattta ttggtgagaa ctacgttcca
      361 tggcaggagt tcgtcaacac ggttggaggg ccattcaggc agtatgctca gaatatggcg
      421 cagatacgct tccggatcga taccgttcag acggcaggtg cctgcttttc cggcaacaag
      481 caggaatctc agcgctgaag caggaattag aggtaaaaac gccctaccgg gccatgaatc
      541 acccggtcat tggagtagta accaaagcag atttagccag catggaacaa atatctttgg
      601 tgaaaagttg gttacgggaa gctggagcgc acaacgtgtt agtaaccagt gcagttaata
      661 acaatggagt tacagaactt tttgccttgc tgcatacaga agaaggctgt tgttaatttc
      721 gattggttct cagatgagaa tgatgacggt gatacacaga taagaatcaa tgagcaatga
      781 gggttgccgg gcaaccctca ttgaataaaa cgggaattac ttcgcttcgc cgtttttcat
      841 ttcgcccata gctttcagct ttttggagat atcgcggcgt tctttggaga gctcggcatt
      901 tttgatgatg taatcgtcaa cgcgatcttc atagtcacct ttcatgctgg cgatgatgcc
      961 ctggattgct tcaacgctca tccctggctt gatgtaatcg ctcaggttgt ccagcaacag
     1021 gacgcgcttc tggttgtcac ggatcttttt ctcaacgtcc tgaatttcac gttgcagttt
     1081 gttcttacga cggaacagac gaacaaattc cagtacgtcc tggaatgaag gcttggtagt
     1141 ttccattttt atacccctga taatgtgaga gtcggattcg tttaagcaac cgctattcgt
     1201 tagggccaac attactcata gctgccgcag atgacaatgc ttttatccct tttcactatc
     1261 atacccttta tccttgctga atcgaagcag cagcaagatg attctgaagt tcaggaatta
     1321 tttgcgcaag gcccggcaaa tcagattcga tagcaactca agctgatgtg ccgtttccat
     1381 gttcagccac acataaccat agattggtgt ttccacgact ttcaggtcat ggtggttatc
     1441 gagcaactga cgcagctctt ccacagcatc gttcaatttt tccgtattgg caaaaaccgg
     1501 ctgcggatta ccttcgtaca gcgcatgaac aaggctcagc agtatttgct gcatcatgtg
     1561 ctgggtatca caaagttttt gcgcgttcaa taacacgaaa tg
//
LOCUS       DAZXUK010000462         1594 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZXUK010000462 DAZXUK010000000
VERSION     DAZXUK010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.466"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..567)
                     /gene="spa29"
                     /locus_tag="ACH3L8_004328"
                     /gene_synonym="spaR"
     CDS             complement(<1..567)
                     /gene="spa29"
                     /locus_tag="ACH3L8_004328"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFT4087156.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPV"
     gene            complement(567..827)
                     /gene="spaQ"
                     /locus_tag="ACH3L8_004329"
     CDS             complement(567..827)
                     /gene="spaQ"
                     /locus_tag="ACH3L8_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFT4087157.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            complement(842..1492)
                     /gene="spa24"
                     /locus_tag="ACH3L8_004330"
                     /gene_synonym="spaP"
     CDS             complement(842..1492)
                     /gene="spa24"
                     /locus_tag="ACH3L8_004330"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFT4087158.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
BASE COUNT          644 a          310 c          245 g          395 t
ORIGIN      
        1 aacaggactg gctaaaataa cagcctgact tgccaataaa gtcagaaatg ttaagatatt
       61 tgagacacgg aaagaacatt gagaaaataa cgggcatata ttataagaca attgtatgga
      121 ttctaaaata aagaccatac caccactgta tagaaataca actgcagaaa aaagattgaa
      181 aaattttgcc aactcagacg tatcaacacc attggcagga tcaattgaac tactaagcgt
      241 tgccccacgc tggttgtcaa taatgctacc aacagcatga aatatccaaa agggaagaga
      301 caagcaaaaa gagagaagga ggccaacaat tatttccttg aacataagga aataaacatg
      361 ttcaaaaaca gaagaaccta tgtctacctt accagaagta attaatcctg atgcaacaag
      421 aaaaatcaca ggtattctaa tagatggaga aattataccg ttatttaaaa atggaagaaa
      481 gaaaaataat ggagccaatc taaaaaaaac gccgttcagg agtattaaaa acacatgaat
      541 actttcgaac cagcttgaaa tgtccatcaa accccactct taattaaaaa cattatttca
      601 tgacaaaaag acaataaaac ctcaccatac catccagaaa gaagtagcaa agatattgag
      661 acacctataa gctttatacc aaaaggaagt gtctgctctt ggagttgagt cactgtctgt
      721 aacaaaccaa tagataatcc aataaccgta gctataccta ctggccataa ggaaaagata
      781 aggattaaat aaagagcctt attacccata taaactatat cagacacatt acctccatca
      841 tctaagcagg aatattgata tattgttcaa tcaaggcctt agacaggata ccccacccat
      901 caagcgcaac aaataataca agttttattg gtactgatat tgtgattgga ctcatcatca
      961 tcataccaag tgcaagtaaa atgcttgaaa taacaagatc aacaacaaca aatggcaaat
     1021 ataaataaaa acctattttg aacgcatctt tgatttcact tagcgcatat gcaggcaaga
     1081 gggaaaacaa tgaataatca ttattctctg cactctttaa atcagcattt tcttcttcag
     1141 acctttggaa aaaacgtgct aactcaagat ctgtatgctt tttcaagtat tgcttatatt
     1201 ccatcaagcc agaatcagaa aaacggacaa tatcagatat tgtatcaaat ttttgagggc
     1261 catttagata attttcataa ccagcttcaa taatgggttt catcacgaac aaagccataa
     1321 tcaatgcaat accattgaga gtcatatttg atggaacctg ctgcaaacca agtgcatttc
     1381 gtaccattac aaaaacaata gaaaacttta tataacaggt accagcagca actaaaaatg
     1441 gtaatagcgt aaaaaaagaa agtgtagcga tgagggacat gtcactcagc atgagattac
     1501 tcctttacca tccaagaact aatctcgata ccataaccat cctcaataga aacaagttcc
     1561 ccatgcccaa ccaaagaacc atttacttta atat
//
LOCUS       DAZXUK010000463         1588 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZXUK010000463 DAZXUK010000000
VERSION     DAZXUK010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.467"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            21..1223
                     /gene="virA"
                     /locus_tag="ACH3L8_004331"
     CDS             21..1223
                     /gene="virA"
                     /locus_tag="ACH3L8_004331"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFT4087159.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            1402..>1588
                     /locus_tag="ACH3L8_004332"
     CDS             1402..>1588
                     /locus_tag="ACH3L8_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFT4087160.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT          561 a          329 c          270 g          428 t
ORIGIN      
        1 aatacatcag gagaaatcaa atgcagacat caaacataac taaccatgaa agaaatgact
       61 catcatggat gtcaactgtc aaatcaacta cagaagtaag ttggaataaa ttgagttttt
      121 gtgacatttt actgaaaata ataactttcg gcatatattc tccacacgaa actctagccg
      181 aaaaacatag cgaaaaaaaa ctaatggata gtttctcacc atcactatca caagataaaa
      241 tggacggaga gtttgcccac gcaaacatag atggcatttc aatacggcta tgccttaaca
      301 aaggcatatg ttcggttttt tatcttgatg gagataaaat acaatccact caactttcat
      361 caaaagaata taataacttg ttaagttcac tcccccccaa acaattcaac ttaggaaaag
      421 tacatactat tacagctccg gtatcaggga attttaaaac tcacaagcct gcaccagaag
      481 ttattgaaac agccattaat tgctgtacat cgataatacc aaacgatgat tacttccacg
      541 ttaaagacac tgattttaat agtgtttggc atgatattta cagagatatt cgagcatctg
      601 acagcaatag tacaaaaata tatttcaaca atattgaaat accactaaaa ttaattgcag
      661 atctaattaa tgagttagga attaatgaat tcattgatag caaaaaagag cttcaaatgc
      721 taagttacaa ccaagtaaat aaaataatca attcaaactt cccccaacaa gacctatgtt
      781 ttcaaacaga aaaacttttg tttacttcac tctttcaaga ccctgcattt atatctgcac
      841 taacatctgc attctggcaa tctcttcaca tcacgtcttc ctctgtggaa cacatatatg
      901 cccaaattat gtccgaaaac atagaaaaca ggcttaattt catgcctgaa caacgagtta
      961 ttaacaattg tggacatatt ataaaaatca acgcagttgt ccctaaaaac gacactgcaa
     1021 tctctgcctc tggggggagg gcttacgaag ttagctcatc aatattacca tcgcacataa
     1081 catgtaatgg tgttggtatt aataaaatag aaacaagcta tctcgttcat gctggaacac
     1141 ttccaagcag tgaagggctt cgcaatgcta tccctccaga aagtcgtcaa gtatcatttg
     1201 ccatcatatc tcctgatgtt taagccaata ttatattggc tgccagactg taaaaaaacg
     1261 ttcttcagaa cagctctata aactattaaa ccaaagctaa aaggatgggg accaatagaa
     1321 tctcagccca tcctttttat atcattatga accgccccgg aaatcctgga gactaaactc
     1381 cctgagaaag aggtaaacag gatgactaaa aatacacgtt tttcccctga agtccgtcaa
     1441 cgggcagttc gtatggttct ggaaagtcag ggcgaatatg actcacaatg ggcagcgatt
     1501 tgttccattg ccccaaagat tggctgtata ctggaaactc tgcgtgtctg ggtacgccag
     1561 catgagcggg ataccggggg cggtgagg
//
LOCUS       DAZXUK010000464         1581 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZXUK010000464 DAZXUK010000000
VERSION     DAZXUK010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.468"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACH3L8_004333"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACH3L8_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFT4087161.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1581)
                     /locus_tag="ACH3L8_004334"
     CDS             complement(1269..>1581)
                     /locus_tag="ACH3L8_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HFT4087162.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          482 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac a
//
LOCUS       DAZXUK010000465         1580 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZXUK010000465 DAZXUK010000000
VERSION     DAZXUK010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.469"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(102..545)
                     /locus_tag="ACH3L8_004335"
     CDS             complement(102..545)
                     /locus_tag="ACH3L8_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFT4087163.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            702..>1580
                     /gene="metA"
                     /locus_tag="ACH3L8_004336"
     CDS             702..>1580
                     /gene="metA"
                     /locus_tag="ACH3L8_004336"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFT4087164.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYV"
BASE COUNT          378 a          443 c          362 g          397 t
ORIGIN      
        1 aaaggctatc cgtcaggatg gcctttttgc gttggtgaaa aaaatgccgg gtgcgacgct
       61 ggcgcgtctt ctccagcttg ggcaggcacg gcagaggcgg cttattcccc cacatacgcc
      121 agattcagca acggatacgg tttccccaaa tcgtccacct cagagcgtcc cgtaacctta
      181 aaacccacct tcttatagaa cccaaccgcc tgctcatttt gctcattaac gttggttgtc
      241 agttccggtg ccatcgagag cgcatgctcc accagcatcc gacccacgcc gcagccgcac
      301 acatcaggat cgataaacag cgcatccata tgctgcccac ttaacaacat aaacccaacc
      361 ggctgctccc gttcattaac cgcgacccac aacggtgctt ccggcagaaa ggaacgcacc
      421 agctcttcca gctcggcccg atactctgct gataaaaaat cgtgggtggc atcgacagat
      481 cgacaccaaa tcgcaacgag ttcctcccct tcctcatgcc gtgagcggcg aatactaata
      541 accattttct ctccttttcg tcattcttat attctaacgt agccttttcc ttgaaacttt
      601 ctcaccttca acatgcaggc tcgacattgg caaattttct ggttatcttc agctatctgg
      661 atgtctaaac gtataagcgt atgtagtgag gtaatcaggt tatgccgatt cgtgtgccgg
      721 acgagctacc cgccgtcaat ttcttgcgtg aagaaaacgt ctttgtgatg acaacttctc
      781 gtgcgtctgg tcaggaaatt cgtccactta aggttctgat ccttaacctg atgccgaaga
      841 agattgaaac tgaaaatcag tttctgcgcc tgctttcaaa ctcacctttg caggtcgata
      901 ttcagctgtt gcgcatcgat tcccgtgaat cgcgcaacac gcccgcagag catctgaaca
      961 acttctactg taactttgaa gatattcagg aacagaactt tgacggtctg attgtaaccg
     1021 gtgcgccgct gggcctggtg gagtttaatg atgtcgctta ctggccgcag atcaaacagg
     1081 tgctggagtg gtcgaaagat cacgtcacct cgacgctgtt tgtctgctgg gcggtacagg
     1141 ccgcgctcaa tatcctctac ggcattccta agcaaactcg caccgacaaa ctctctggcg
     1201 tttacgagca tcatattctc catcctcatg cgcttctgac gcgtggcttt gatgattcat
     1261 tcctggcacc gcattcgcgc tatgctgact ttccggcagc gttgattcgt gattacaccg
     1321 atctggaaat tctggcagag acggaagaag gggatgcata tctgtttgcc agcaaagata
     1381 agcgcattgc ctttgtgacg ggccatcccg aatatgatgc gcaaacgctg gcgcaggaat
     1441 atttacgcga tgtggaagcc ggactagacc cggatgtacc gtataactat ttcccgcaca
     1501 acgatccgca aaacaaaccg cgagcgagct ggcgtagtca cggtaatttg ctgtttacca
     1561 actggctcaa ctattacgtc
//
LOCUS       DAZXUK010000466         1579 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZXUK010000466 DAZXUK010000000
VERSION     DAZXUK010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.470"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            6..1211
                     /gene="tet(B)"
                     /locus_tag="ACH3L8_004337"
     CDS             6..1211
                     /gene="tet(B)"
                     /locus_tag="ACH3L8_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFT4087165.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1324..>1579)
                     /locus_tag="ACH3L8_004338"
     CDS             complement(1324..>1579)
                     /locus_tag="ACH3L8_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFT4087166.1"
                     /translation="MPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKN
                     LLNGWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          387 a          294 c          357 g          541 t
ORIGIN      
        1 gtgaaatgaa tagttcgaca aagatcgcat tggtaattac gttactcgat gccatgggga
       61 ttggccttat catgccagtc ttgccaacgt tattacgtga atttattgct tcggaagata
      121 tcgctaacca ctttggcgta ttgcttgcac tttatgcgtt aatgcaggtt atctttgctc
      181 cttggcttgg aaaaatgtct gaccgatttg gtcggcgccc agtgctgttg ttgtcattaa
      241 taggcgcatc gctggattac ttattgctgg ctttttcaag tgcgctttgg atgctgtatt
      301 taggccgttt gctttcaggg atcacaggag ctactggggc tgtcgcggca tcggtcattg
      361 ccgataccac ctcagcttct caacgcgtga agtggttcgg ttggttaggg gcaagttttg
      421 ggcttggttt aatagcgggg cctattattg gtggttttgc aggagagatt tcaccgcata
      481 gtcccttttt tatcgctgcg ttgctaaata ttgtcgcttt ccttgtggtt atgttttggt
      541 tccgtgaaac caaaaataca cgtgataata cagataccga agtaggggtt gagacgcaat
      601 cgaattcggt atacatcact ttatttaaaa cgatgcccat tttgttgatt atttattttt
      661 cagcgcaatt gataggccaa attcccgcaa cggtgtgggt gctatttacc gaaaatcgtt
      721 ttggatggaa tagcatgatg gttggctttt cattagcggg tcttggtctt ttacactcag
      781 tattccaagc ctttgtggca ggaagaatag ccactaaatg gggcgaaaaa acggcagtac
      841 tgctcggatt tattgcagat agtagtgcat ttgccttttt agcgtttata tctgaaggtt
      901 ggttagtttt ccctgtttta attttattgg ctggtggtgg gatcgcttta cctgcattac
      961 agggagtgat gtctatccaa acaaagagtc atcagcaagg tgctttacag ggattattgg
     1021 tgagccttac caatgcaacc ggtgttattg gcccattact gtttgctgtt atttataatc
     1081 attcactacc aatttgggat ggctggattt ggattattgg tttagcgttt tactgtatta
     1141 ttatcctgct atcgatgacc ttcatgttaa cccctcaagc tcaggggagt aaacaggaga
     1201 caagtgctta gttatttcgt caccaaatga tgttattccg cgaaatataa tgaccctctt
     1261 gataacccaa gagggcattt tttacgataa agaagattta gcttcaaata aaacctatct
     1321 attttattta tctttcaagc tcaataaaaa gccgcggtaa atagcaataa attggccttt
     1381 tttatcggca agctctttta ggtttttcgc atgtattgcg atatgcataa accagccatt
     1441 gagtaagttt ttaagcacat catcatcata agctttaagt tggttctctt ggatcaattt
     1501 gctgacaatg gcgtttacct taccagtaat gtattcaagg ctaatttttt caagttcatt
     1561 ccaaccaatg ataggcatc
//
LOCUS       DAZXUK010000467         1576 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZXUK010000467 DAZXUK010000000
VERSION     DAZXUK010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.471"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            293..421
                     /locus_tag="ACH3L8_004339"
                     /pseudo
     CDS             293..421
                     /locus_tag="ACH3L8_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            611..1015
                     /locus_tag="ACH3L8_004340"
     CDS             611..1015
                     /locus_tag="ACH3L8_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087167.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            1078..1576
                     /locus_tag="ACH3L8_004341"
                     /pseudo
     CDS             1078..1576
                     /locus_tag="ACH3L8_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          449 a          372 c          410 g          345 t
ORIGIN      
        1 tatttaatac agaacagtcg ggattttatg aaatcaatga tgtttatgtc agaaagtaat
       61 aatcctctcc cacaaagtga attaaatgaa tatataatgc gttcgaagta tatgtattga
      121 gctgtcatac tgagaaacaa accttagggc agcacaatta aagccagaaa caactatgca
      181 agaaagttac taaataagtg ctcggaacag taaaacactc aggggggact cagtccggat
      241 tatgtaatca gataaattgc caaacatcac aaatcaccag ccggactgag tgatgccgag
      301 cattgcacca ataccccgtg atgaacgatg cctgatgcag aaagctccct ataaaacaaa
      361 gaacaaaaac tacgaccggc gattcaccgt catactgatg ctgcatcgtg gtgacactgt
      421 ctgtcacttt catgttgact gaacaactct gctgcgaaga aggtgtaccg ccggcgggat
      481 ttgattgtcc ggcgtctagc ttccacttcc ctgatggaac tgaaagttgt tatggccggt
      541 cagcttgccc tgtccgcagg acatgaggtg ctgtgacgga cagcaccagt aacggaaagt
      601 gacaatgaaa atgaatactg cgctgatagt tgcactaatg tgtatgtggt atgcggtacc
      661 cgctgctgca aaagagactt tactggctat gccacgtaat tcaacagaac attgctatgc
      721 ggagattaat gtgcacggac catatggggt gtatttccga gtggtcccgc atccccccgg
      781 gggaaagagc tgggtcgaat gtaacagtga ctactattac tctgataaac caccgggagt
      841 acagatactc ggaacccgtg cagggtgtcg ggtatacgga atctgcggga ccacctcgac
      901 actgcatgtg gctggaagag gggttgtgtg tattaaaaat atatgcagtc cacgtgggat
      961 gataattcac aggatacgga aacgtcctgt agtggctgta agcgatgaga tgtgatctta
     1021 cccagcaata gtagacacgc ggctaagtga gtaaactctc agtcagaggt gactcacatg
     1081 acaaaaacag tatcaaccag caaaaaaaca cgtaaacagc attcgcctga attttgcagt
     1141 gaagccctga agcttgctga acgcatcggt gttgctgccg cagcccgtga actcagcctg
     1201 tatgaatctc agctctatgc gtggcgcagc aaactgcagc aacagatgac ctcgtctgag
     1261 cgtgaaagtg aactgcccgc ctgaagcggc aactggcgga gcaggcagag gaactggcta
     1321 tcctccaaaa ggccgcgaca tactttgcgc agcggctggt atacgtggtg tcagcgtcgg
     1381 acaggaataa gccctcgtca gcagttccgt cagcactgcg acagcgttgt cctcgcggcg
     1441 gcctttaccc ggtcaaaaca gcgttacggt gcaccacgcc tgacggatga actgcgtgct
     1501 cagggttacc actttaacgt aaaaaccgtg gcggcaagcc tgcgccgtca ggggctgagg
     1561 gcaaaggcct cccgga
//
LOCUS       DAZXUK010000468         1545 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZXUK010000468 DAZXUK010000000
VERSION     DAZXUK010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.472"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(50..125)
                     /locus_tag="ACH3L8_004342"
     tRNA            complement(50..125)
                     /locus_tag="ACH3L8_004342"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90..92),aa:Ile,seq:cat)
     gene            complement(302..991)
                     /locus_tag="ACH3L8_004343"
     CDS             complement(302..991)
                     /locus_tag="ACH3L8_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFT4087168.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(988..1347)
                     /locus_tag="ACH3L8_004344"
     CDS             complement(988..1347)
                     /locus_tag="ACH3L8_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFT4087169.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(1347..1535)
                     /locus_tag="ACH3L8_004345"
     CDS             complement(1347..1535)
                     /locus_tag="ACH3L8_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087170.1"
                     /translation="MPFYFRKECPLNSGYLNMVPALRELMQAKHRAQKAVDELVDCVA
                     ELETRVGKFEKLVQEVLR"
BASE COUNT          372 a          431 c          324 g          418 t
ORIGIN      
        1 accgtcaact tagaaggttg atgctctatc cggatgagct agtggtgttt ggtggccctt
       61 gctggacttg aaccagcgac ctggcgatta tgagtcgctc gctctcacca ctgagctaaa
      121 gggccggagg cagaataata accatatgtc atcacatctg caaactcatc tgaccaccag
      181 cacgtttaac gtcctgtgcc atttttcagg cataaaaaaa ccgctcggcg gcgggttttg
      241 cttactttac catcgcgtac aaaatcggca aagtatcaga tttgcatgag atatatgcct
      301 tttaatctac ttttgcaata ctttgctttg aaaatgccgc tttttgttct gaacgtgttc
      361 tcatcacaaa caataaagcc tcactatcca gtcggtgaaa aatgtgtttc attacaaccc
      421 agtgaccagt aaatgttttg gaccagtttt tggttgttac tcccgccagt aatgccagct
      481 cctgatattc ataaccttcc ccaccaaaaa gttctgcttt taccgcctgc gccgccagcc
      541 agattaattt tttcaggcgt tcctgtgttt tctctgcaat tttcctagtg ccagaatgag
      601 ccttaaactc ttcccacgcc cagtgggtta tggtgatctg atactcccag caaatacttc
      661 cgctgtaaca ccacaacaac caggctttat agtgctcttc aagagacaga acagcccgcc
      721 gccacgatga tgtcgaaaac gcaacctgac tgaccagagg aattgacgcc cccttcgcca
      781 gtgattgttt tcccgggatt ggtggattat cccgcgttat catttttccg gtcacctcat
      841 cgcggtaccg gattttttta cgcctgtaac gccctgtatc gaacatggca ttctcctgcc
      901 aggcttcaag ctgacctttt gtcgccccac tcaaatcagc ggtggcgata atgagctgct
      961 cacgaacaaa ctgtaaatac tggttattca tgcacactcc agctctgtga tttttatccc
     1021 caaccgacca ccaggaacag gcagtccgcg cacaatattg gtttcatcaa actgctcgtc
     1081 gtcgataagc aaccccgcat gtgtcagtgc atccagtggt gctttcagga tattgtccag
     1141 gtcacgacgc cgcttatccg gtggctctgc aataatcttt atcgccagtc ttccggacag
     1201 gcttaatttc agtcgctgct gacggacaat aagcgccaca tcccggcgat aacgctcacc
     1261 ggcttttgat acaaaatatg tgctgccacg acgtcgccag taagtattca ccgtcggcgg
     1321 ataaggtaaa ataaactcat ggcgcatcag cgcagcacct cctgcaccag cttttcaaac
     1381 tttccgactc tggtttccag ctctgccaca cagtccacca gctcatctac tgctttctgt
     1441 gcgcgatgct tcgcctgcat cagttcccga agcgcgggta ccatattgag gtagcctgag
     1501 tttaacggac actccttcct gaaatagaat ggcatcagaa ggagc
//
LOCUS       DAZXUK010000469         1544 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZXUK010000469 DAZXUK010000000
VERSION     DAZXUK010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.473"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            39..784
                     /gene="nfeF"
                     /locus_tag="ACH3L8_004346"
                     /gene_synonym="yqjH"
                     /pseudo
     CDS             39..784
                     /gene="nfeF"
                     /locus_tag="ACH3L8_004346"
                     /gene_synonym="yqjH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
     gene            complement(838..913)
                     /locus_tag="ACH3L8_004347"
     tRNA            complement(838..913)
                     /locus_tag="ACH3L8_004347"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(878..880),aa:Ile,seq:cat)
     gene            1038..1544
                     /gene="mug"
                     /locus_tag="ACH3L8_004348"
                     /pseudo
     CDS             1038..1544
                     /gene="mug"
                     /locus_tag="ACH3L8_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT          361 a          382 c          432 g          369 t
ORIGIN      
        1 tttagatata tctgattacc atcacgaagg cgataacaat gaataacacc ccccgctacc
       61 cgcagcgcgt tcgcaatgat ctgcgcttcc gtgaactgac tgtgctacgc gttgagcgca
      121 tcagcgccgg ttttcagcgc attgtccttg gcggcgaggc gttggacggt tttacatcgc
      181 gcggctttga cgatcacagc aaactcttct ttcctcaacc tgacgctcac tttgtgccgc
      241 caacggtaac ggaagagagc atcgtctggc cggaaggacc acgcccaccg tcgcgtgact
      301 atacgccact gtatgacgaa ctacgccatg aactggcgat cgatttcttt attcacgacg
      361 gtggggtcgc cagcggctgg gcgataaact tacggtggca ggtccgcgcg gttcgctggt
      421 ggtgccggaa gattacgcgt atcagctgta tgtctgcgat gaatccggaa tgcccgcact
      481 gcgccgccgc ctggaaacgt tgagcaaact tgccgttaaa ccgcaagtta gtgcgctggt
      541 tagcgtgcgg gataacgcct gtcaggatta tctcgcgcac cttgatggtt ttaatatcga
      601 atggctggca catgatgagc aggcggtaga tgcgcgtctg gcgcagatgc aaatccctgc
      661 cgatgattac ttcatctgga taaccggcga aggtaaagtc gttaagaatt taagccgccg
      721 ctttgaagcg gaacagtatg acccacagcg ggtacgtgca gcggcttact ggcacgcaaa
      781 gtaactgata tataaaaaga aaaaggctga cgatttctcg tcagcctttg ctatatctgg
      841 tggcccctgc tggacttgaa ccagcgacca agcgattatg agtcgcctgc tctaaccact
      901 gagctaaggg gccatggtag cggattataa agtaactccg tgccgcaatc cagccattac
      961 cgcgcgcctg ctgtttttat aaacaatgta ttttcaatcc gttatacttt tcctggtgat
     1021 gttagaaagg agtaaatatg gttgaggata ttttggctcc agggttacgg gtcgtgtttt
     1081 gcggtatcaa ccctgggctt tcatccgccg ggactggttt tccctttgct catccggcaa
     1141 atcgcttctg gaaggtgata tatcaggccg ggtttaccga ccgtcagttg aagccgcagg
     1201 aggcacagca tctgctggat tatcgttgtg gcatcaccaa actggtagac cgtccaacgg
     1261 tgcaagccaa tgaagtttca aagcaggagc tgcacgcagg tgggcgtaag ctgattgaaa
     1321 aaattgaaga ttatcagccg caggcgttgg ctattctggg caaacaagca tatgaacagg
     1381 gattcagcta gcgcggtgca cagtggggga aacaaacgct caccattggt tcgacgcaga
     1441 tttgggtgct gccaaatccc agcggtttaa gtcgcgtttc actggagaaa ctggttgaag
     1501 cgtatcgcga gctggaccag gcgctggtag tgcgtgggcg ataa
//
LOCUS       DAZXUK010000470         1542 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZXUK010000470 DAZXUK010000000
VERSION     DAZXUK010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.474"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            99..836
                     /gene="erm(B)"
                     /locus_tag="ACH3L8_004349"
     CDS             99..836
                     /gene="erm(B)"
                     /locus_tag="ACH3L8_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFT4087171.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          566 a          268 c          243 g          465 t
ORIGIN      
        1 ctaatcttat gactttttaa acagataact aaaattacaa acaaatcgtt taacttctgt
       61 atttgtttat agatgtaatc acttcaggag taaattacat gaacaaaaat ataaaatatt
      121 ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa
      181 aagaaaccga taccgtttac gaaattggaa caggtaaagg gcatttaacg acgaaactgg
      241 ctaaaataag taaacaggta acgtctattg aattagacag tcatctattc aacttatcgt
      301 cagaaaaatt aaaactgaat actcgtgtca ctttaattca ccaagatatt ctacagtttc
      361 aattccctaa caaacagagg tataaaattg ttgggaatat tccttaccat ttaagcacac
      421 aaattattaa aaaagtggtt tttgaaagcc atgcgtctga catctatctg attgttgaag
      481 aaggattcta caagcgtacc ttggatattc accgaacact agggttgctc ttgcacactc
      541 aagtctcgat tcagcaattg cttaagctgc cagcggaatg ctttcatcct aaaccaagag
      601 taaacagtgt cttaataaaa cttacccgcc ataccacaga tgttccagat aaatattgga
      661 agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta
      721 aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt accgttactt
      781 atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg aaataattct
      841 atgagtcgct tttgtaaatt tggaaagtta cacgttacta aagggaatgt agataaatta
      901 ttaggtatac tactgacagc ttccaaggag ctaaagaggt ccctagacta gcaagaagta
      961 cacaagaagc cttaaagatt atagaaaagc ttttccatga aatgcttaac attatttaat
     1021 gttaacatgt ggctttgtgg taactaccac gagtggtaac ttgtaacatg aaaaaagtta
     1081 caagttacaa gtggtttatt tacgaacata aaggctagga aagcacttga taagtatatc
     1141 aagtgctttt tttccgttcg attttactat ccaagatacc gcttgacgta cctaatattt
     1201 tagtgtgcta agggctttgc tttttctggt tatagttcgt tcaaaaaaga ccgcaagggt
     1261 aaatgcacga tcatagatcg cccttgcagc tttttaatct ctccactttt ttataaccaa
     1321 gcaaaccacc aagcacactg aaaaaataat taggaactaa agcgaagtgc gtatcttgga
     1381 taaatcttca ctcgatttta aaagattaag cacccagcca aagcgcctaa aaaaataatt
     1441 tttttaaaga aaggaagtaa tttaaatgac tacaatttta agcgactaca ataaatctat
     1501 cctaattgaa ttactaaaca ccaatcgcca aattatcgtc gt
//
LOCUS       DAZXUK010000471         1529 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZXUK010000471 DAZXUK010000000
VERSION     DAZXUK010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.475"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            14..977
                     /locus_tag="ACH3L8_004350"
                     /pseudo
     CDS             14..977
                     /locus_tag="ACH3L8_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            1022..1525
                     /locus_tag="ACH3L8_004351"
     CDS             1022..1525
                     /locus_tag="ACH3L8_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFT4087172.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT          447 a          310 c          343 g          429 t
ORIGIN      
        1 agggagtttt tttatgaaaa tgaggtcatt tacccgctca ttagtctgcg catccttgct
       61 ggctctggtt agcactggcg taaacgccgc agaaaaagtg gcgttaaagt tagcacacaa
      121 cctggaaaga agtcatgttg tacatcagag ttttgaagag ctggcgaaag aggtaaaaca
      181 gctgtcaaaa ggtaatatgg tgatccgtat ttatcccagc agccagatgg gtaatgcgcg
      241 agaaactatg gaattgttgc aaaatggcgc actggatatg accaaaggtt ccgccagtga
      301 tctggagtct tttgataata tttatgctat ctataatttg ccgttcttat taagttgttt
      361 ttggtgaagt tggtaaagaa attatggact caacaaaaga caaaggcttt tttgctttat
      421 cagcttatgt agcgggtact cgcagtttct atgcgaaaaa accgattaca aagccagaag
      481 atcttaaagg actgaaaatt cgtgttcagc caagtccgac caccatcaaa atgattgaac
      541 tgatgggtgg ttcacccact ccaatttcat ttggcgaagt ttatactgcg atgcagcaag
      601 gtgtagtaga tggcgcagaa aataacgtgc cttcctggat gcagactcga catattgaaa
      661 ttgccaaagt tttttctgaa gatgaacacg cctcaattcc tgactttttg gttatctcca
      721 ccaaaacatg gaataaatta accccagaac agcaacaaat cctcgaaaca gcggcgaaga
      781 aatccgaagc ttatcaacaa aaattgtggg aaaaaattga cgctgacaca cgtgctcagg
      841 cgaaagctat ggggggtgaa attgtaaaag tggataaagc gccattccgt aaagccgtgc
      901 aacctctgtt tgacgacttc aaaaaagatc caaaacaagc tgctttgtta gagaaattcg
      961 acaacgctgc tcaataatat tccgggccag cctctggccc tgattttcca ggatgcttac
     1021 aatgatattt aaccgcctga aacttgcggt agaccgcgtc attgccacgt tttctgtcgc
     1081 cgtaatgctt gctctggttg tatgcgtagt ctggcaagta tttagccgct acgtacttaa
     1141 ccagccaagt acattaaccg atgagctggc acgctttttg atgatctggg taggattatt
     1201 aggtgctgca tataccgtcg gcgcgcagcg acatctctct attgatttat ttgctctcgc
     1261 cctgaataag cgcaaacaat tacttcttag tatagtcatt aacgttctca ttttaggttt
     1321 tgccggttcg gtcattgtga ctggtggcct gaaattaatt gataaaacac tggcaacatc
     1381 acaggtatct gccgctatgc aaataccgat gggatacgta tatatcattc tgccattaag
     1441 tggagtggta atgatgtttt atgccttgtg ttttattaat caaagcattc aacaactgaa
     1501 acaacctgcg caggaggcca gttaatgga
//
LOCUS       DAZXUK010000472         1512 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZXUK010000472 DAZXUK010000000
VERSION     DAZXUK010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.476"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            65..907
                     /locus_tag="ACH3L8_004352"
     CDS             65..907
                     /locus_tag="ACH3L8_004352"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFT4087173.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            910..>1512
                     /gene="capU"
                     /locus_tag="ACH3L8_004353"
     CDS             910..>1512
                     /gene="capU"
                     /locus_tag="ACH3L8_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFT4087174.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHE"
BASE COUNT          458 a          276 c          331 g          447 t
ORIGIN      
        1 taaagttcac cacctttatt aggctttcct tttatctcta cttactgggt gccagaaact
       61 ttaaatttta aacggagggg gcattttgtt taaagcaaat catgttccgg tattaatgta
      121 tcatcatgtc agccattgcc caggactggt tacactttct cccgagacat tccggaaaca
      181 aatgaaatgg ttggctgaaa acaactggaa aacactgtca tcagatgaac tggagttttt
      241 ttatcggggc ggaaaacttc cccgtaaaag cgttatgttg acatttgatg atggatacct
      301 tgacaactgg tttcaggtat atcctttatt aaacgagttt aatctcaagg cacatatttt
      361 tttaattacc agttttattg gaaatggtcc ggttcgtcac tctcctggaa aggaatattc
      421 tcatcgcgat tgcgaacatc aaatagcaac cgggaatgct gataatgtta tgcttcgctg
      481 gtctgaagtg aacgaaatgt tgcaatctgg tctggtcgaa tttcatgttc atacacatac
      541 tcatacacgt tgggataaaa agttcacttc acgagaagaa cagtgtaaac atcttcggca
      601 ggacttattg agcgggagag aatatttaaa aaaaatgaca ggaaaatgca gtaaacattt
      661 gtgttggccg gaaggttatt ataacaagga ttatattcag atcgctgaag aacttggatt
      721 ccattattta tatacaacag aaagaagaat gaatgctcct gctaaaggaa ctacacggat
      781 aggaagaata agcaccaagg aacgggaaaa ttgcgtatgg ctgaaaagac gactttttta
      841 ttatacaaca ccgttttttt catcgttgct ggcccttcat aaggggccaa gattaccgga
      901 tgactaataa tgaatatact atttacggaa tcatcaccga atataggtgg tcaggaatta
      961 caggctgttg ctcaaatgaa ggccctgaag aaaatggggc attcagttct gcttgtctgc
     1021 agggagaaca gcaaaattgc ttttgaagcc agtaagtttg gaattgatat cacattcgcg
     1081 ttatttcgaa acagtcttca cattcctact gtatggagat tactcggaat agttcatagt
     1141 tttcagcccg atgcaatcgt ttgtcacagt ggacatgata gtaatattgt tggtttggta
     1201 cggtttttta cctggaaaca tccattcaga attatcaggc aaaagacata tttgacacga
     1261 aaaacaaaat ttttttcaat aaatcatttt tgcgatgagg tgattgttcc cggaacaaat
     1321 atgaagacac atctggagca ggaaggatgt cgaactcggg ttactgttgt gcctcctggc
     1381 tttgatttcc aggaattata cgttgattcc cgaaacagtt tgcctccgag tgttctttcc
     1441 tggctggcgt cccgaagagg atgccccgta attgctcagg taggaatgtt gcgcccggaa
     1501 aaagggcacg aa
//
LOCUS       DAZXUK010000473         1504 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZXUK010000473 DAZXUK010000000
VERSION     DAZXUK010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.477"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            8..307
                     /locus_tag="ACH3L8_004354"
                     /pseudo
     CDS             8..307
                     /locus_tag="ACH3L8_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159793.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(347..>1504)
                     /locus_tag="ACH3L8_004355"
     CDS             complement(347..>1504)
                     /locus_tag="ACH3L8_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HFT4087175.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT          397 a          296 c          357 g          454 t
ORIGIN      
        1 aacggttatc aaagatgttc tattatggat tgaacataat ttagatcagt ctttactgct
       61 tgatgatgtg gcgaataaag cgggttatac caagtggtat tttcagcggc tgttcaaaaa
      121 agtaacaggg gtcacactgg ctagctatat tcgtgctcgt cgtttgacga aagcggctgt
      181 tgagttgagg ttgacgaaaa aaactatcct tgagatcgca ttaaaatatc aatttgattc
      241 ccaacaatct tttacacgtc gatttaagta catttttaag gttacaccaa gttattatcg
      301 gcgtaataaa ttatgggaat tggaggcaat gcactgagag atcccctcat aatttcccca
      361 aagcgtaacc atgtgtgaat aaattttgag ctagtagggt tgcagccacg agtaagtctt
      421 cccttgttat tgtgtagcca gaatgccgca aaacttccat gcctaagcga actgttgaga
      481 gtacgtttcg atttctgact gtgttagcct ggaagtgctt gtcccaacct tgtttctgag
      541 catgaacgcc cgcaagccaa catgttagtt gaagcatcag ggcgattagc agcatgatat
      601 caaaacgctc tgagctgctc gttcggctat ggcgtaggcc tagtccgtag gcaggacttt
      661 tcaagtctcg gaaggtttct tcaatctgca ttcgcttcga atagatatta acaagttgtt
      721 tgggtgttcg aatttcaaca ggtaagttag ttgctagaac ccatggctcc tttgccgacg
      781 ctgagtagat tttaggtgac gggtggtgac aatgagtccg tgtcgagcgc tgattttttc
      841 ggcctttaga gcgagattta tacaatagaa tttggcatga gattggattg cttttagtca
      901 gcctcttata gcctaaagtc tttgagtgac tagatgacat atcatgtaag ttgctgatag
      961 gtttccagtt ttccgctcct aggtctgcat attgtacttt tcctcttact cgacttaacc
     1021 agtaccaacc cagcttctca acggatttat accatggcac tttaaagcca gcatcactga
     1081 caatgagcgg tgtggtgtta ctcggtagaa tgctcgcaag gtcggctaga aattggtcat
     1141 gagctttctt tgaacattgc tctgaaagcg ggaacgcttt ctcataaaga gtaacagaac
     1201 gaccgtgtag tgcgactgaa gctcgcaata ccataagtcg tttttgctca cgaatatcag
     1261 accagtcaac aagtacaatg ggcatcgtat tgcccgaaca gataaagcta gcatgccaac
     1321 ggtatacagc gagtcgctct ttgtggaggt gacgattacc taacaatcgg tcgattcgtt
     1381 tgatgttatg ttttgttctc gctttggttg gcaggttacg gccaagttcg gtaagagtga
     1441 gagttttaca gtcaagtaat gcgtggcaag ccaacgttaa gctgttgagt cgttttaagt
     1501 gtaa
//
LOCUS       DAZXUK010000474         1497 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZXUK010000474 DAZXUK010000000
VERSION     DAZXUK010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.478"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(117..1316)
                     /locus_tag="ACH3L8_004356"
     CDS             complement(117..1316)
                     /locus_tag="ACH3L8_004356"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFT4087176.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT          390 a          413 c          353 g          341 t
ORIGIN      
        1 caatagcgtt aattcgctaa caaaacagcg agttaacacc agaatgtcgg atgcagaacc
       61 gtaccaaaat gacttaataa cgacatatac aaacggctga cgggaaaacg tcatggttat
      121 ttcacaattg acgccaaatc ctgcgcacaa gcccatgcac tcgaccacgc ccactggaag
      181 ttatagcccc ccagccagcc ggtaacgtcc atcacttcgc cgatgaagta cagcccaggc
      241 actttgcgcg cttccatcgt ccgtgaagaa agttcgttgg tgtccacgcc gccgagcgtc
      301 acttcggcag tgcgatagcc ttcagtgccg ttgggttgta cgcgccagtc ggtcaatgtg
      361 ctaatcagtg cctgttggtc acacacgttg agctgtttta gcgaaacatc cgggatttgc
      421 ccgagttgct gtaagcgttc aaccaaccgc ttcggtagat gaaccgccag tgtgtttttc
      481 aggctttgat tcggatgtgc gttacgctgc tcattcagga aggtttcgag gtccacatcc
      541 ggtagcagat tgatactgac aaattcaccc ggttgccagt agcttgaaat ctgcaacacc
      601 gccggtccag ataagccgcg gtgggtgaag agtaagttct cacggaaaac ggtgccgttt
      661 tcagcggtaa tcacggaagg caccgccacg cccgccagca cctgtaactc ttcgagcaac
      721 ggtttatgca gagtgaatgg caccagaccc gcgcgggtcg gcagcacgtt gaggccaaat
      781 tgttcggcaa tcttataacc aaacggcgat gcgcccagcc ccggcattga cagcccaccg
      841 gtcgcgatga ccagcttttc gcaaccgaca gtcatgccgt tcagatcaag cgtgaagcct
      901 gtttcatcct tcgccacact cagcacttcg ctacgcaatc tgaaggttac attgcccttc
      961 tcgcactcat ccaccagcat gtcgacaatc tgctgcgcgg agtcatcgca gaagagttgc
     1021 cctaacgttt tctcgtgcca ggcgataccg tgtttattga ccagatcaat gaaatcccac
     1081 tgggtaaaac gtgcgagtgc agacttacaa aaatgcggat tctggctcag ataagcgcct
     1141 ggttcgacat aaaggttggt aaagttgcag cgcccaccgc cagacataag gattttgcgc
     1201 cctagtattt taccattatc gatcagcaga acccggcgtc ctacctgacc tgccagcgca
     1261 gaacagaaca tacccgccgc accagcgcct ataataatgg catcaaacct ttccacgttg
     1321 cgctcctctt agaaaaaacg ggcgtgaatt gtaaagattc ctcagtggtc gcaccagcat
     1381 caatattact aaaaggaagt atttgcctga attatataag ataattattt tttgagtgaa
     1441 atccatacag gaggcaaatc aaaaaaagtc tatatttcac tttgcccgcg ccgcgaa
//
LOCUS       DAZXUK010000475         1487 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZXUK010000475 DAZXUK010000000
VERSION     DAZXUK010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.479"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(134..616)
                     /locus_tag="ACH3L8_004357"
     CDS             complement(134..616)
                     /locus_tag="ACH3L8_004357"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFT4087177.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(607..909)
                     /locus_tag="ACH3L8_004358"
     CDS             complement(607..909)
                     /locus_tag="ACH3L8_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFT4087178.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1181..1453
                     /locus_tag="ACH3L8_004359"
     CDS             1181..1453
                     /locus_tag="ACH3L8_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087179.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          423 a          372 c          318 g          374 t
ORIGIN      
        1 ttcaggaacc gttttgacgg cttcggcttt aaactccgta gagtaacgtt gggtttttct
       61 gctcattatt ggctccttct gatgccattc tatttcagga aggagtgtcc gttaaactca
      121 ggctacctca gactcagact ttataaaaca aggtattagg tgccacaact gactgaacaa
      181 aaccaaaacg ctcataaaat gctctggcgt catcatcaat tgcatgtacc ataacagcct
      241 taatgccaac ctgatcagcc agattgacaa cccggtgaat ggcatcactt agtagccatt
      301 taccgaaacc atgcccctgg gtacaaacat caacagccag acgccctaac agaacaacag
      361 ggacaggatt gggcatgtta tgccgcaaac tgcgaccaag ctcggcatga gtgacggaac
      421 cagtagctaa tgagtagtat cccacaatac gtaaagtatc ctccaagcaa ataacgaacg
      481 tgcgagaagc attgagcatc tggtttttca tagcccgacc acgtaaccag ttacttagca
      541 cctcatttcc acagtcaaat ggctgaagta tatgctcatc cgtcaacaat gctggcgcgg
      601 tgacatttat ttccattcag gctttaccgc catcagacgt tcacgccctt ctgcattctg
      661 cacaggagct tccagttgcg taataaacga atcataacgt tcgttatcca gcacaaataa
      721 acgttgatcc aggataatgt ttttagcttc ctggtacgcc agttccatga tgaaatcggt
      781 tcggttctta ttgaccaagc tcgcagcata atcaataacg gctcgttcct catcagttgc
      841 ccggatgtta atctgatgtt ctcgcggtgt tttacggacg cttgcagctg tagacataaa
      901 aacctccatt ggtaacaatt tgctatgcaa ttaatgttat cgcaaatggt aacagaacgc
      961 tatacagaat cacttacggg aaaagcgatc ccctgataaa aacaatggca gagacctgaa
     1021 cattgtgacg atgatctgtg tcggcgcaca aatcatgtct gattacggcc gtttaatgtg
     1081 attttgataa tgaaatgcct gaaccacggc agcctggcgg ctgcaaatcg gggtgtggca
     1141 gaatatgagc tttcagagta accatccaga atgagttccc atgtcacaca acctggagca
     1201 ccagaaagtc cacaccagaa tggtgaaaga ggttctgaaa gccgtcgcca gagccaataa
     1261 tcacccttat aagtccgtat ttgcagactt tatcaccggt catccctcct gcacggtctg
     1321 tttctgggaa accttccaca aaatgtaccc ggattcaccg tacgaatacg tcacgttctg
     1381 ccatacctgc cgtcgttttg atttatacga aacagaagcg gagatgaagg cggatgaccc
     1441 gaagtggtgg tgattttcag ccccgaccgc tgaaacgcct gttcaca
//
LOCUS       DAZXUK010000476         1482 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZXUK010000476 DAZXUK010000000
VERSION     DAZXUK010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.480"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..486)
                     /locus_tag="ACH3L8_004360"
                     /pseudo
     CDS             complement(1..486)
                     /locus_tag="ACH3L8_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(508..1048)
                     /gene="yhaB"
                     /locus_tag="ACH3L8_004361"
                     /pseudo
     CDS             complement(508..1048)
                     /gene="yhaB"
                     /locus_tag="ACH3L8_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
     gene            complement(1304..1477)
                     /gene="tdcR"
                     /locus_tag="ACH3L8_004362"
     CDS             complement(1304..1477)
                     /gene="tdcR"
                     /locus_tag="ACH3L8_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFT4087180.1"
                     /translation="MNTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFS
                     SDRTSFCECNRFP"
BASE COUNT          461 a          262 c          276 g          483 t
ORIGIN      
        1 atcgacatta cgtaagtcca gagaggatag atcaactata tctccagtcc cacaactaaa
       61 gattacgttc aacctcctga tttcattgta aatatctttt aatgggcaat taacattgat
      121 attgcctgta gatatttcct agttttttaa cggttcatta atggtaagtt cgcaggtatt
      181 gtccgatcgt tgaataattt gggtgttata acctaaaaaa tccagacgaa gagacgagtc
      241 attataaaga attacatttt gtaagtacgc taattctttt tgaaaatcac cagtgataat
      301 gcattttcca cttggttgta acgcgttaag taaagtgacc atttgaatag aggtctgttt
      361 tagtttttct atacctggtg ctagtgcttc gaggttattt cctatggggc tttcaggaag
      421 gtcattcatt tttcgacgga ctaaatcact actaatatca ttaccaattg atgaaacagg
      481 gaacattatt ttatccttta taaataatca ataaaaatca ttattaaaat gatcgggtga
      541 aaaaaagaga atttgccaac aatctgaagt aatgcaggtt ttattaaagt gcctaggggg
      601 tgttaagatg gcttcttttt gggtgaactt tgcgcaatac ttagaaatcg tctccttaat
      661 acattccagc tcttttttat tgacggctga gcaagtacat tgttcaccaa aaggtgcaaa
      721 agtgcctaca tctaatgttt gtatgatata atcatcatgt tctcctgtag aaatatctat
      781 gatgaatgga ctcatatttt ccggttggat tcggaatcat gacagaagga acatcgcctt
      841 gatagaaatt caatgcagta attgcagata atactctccg gagttcatta cttatactgt
      901 aaattttatt aacgtcattg gtggagacaa tttctgcaaa ctttcttttt accgtccaat
      961 aatcaatatt tctattgtga ttgttaacca ctgcgtgcat tggcgggatt gaagggtgaa
     1021 aaatatgtgc aattgggaag cctttcattg atgcctgcct tgtcgtcatg acaaacaata
     1081 atagatgctt attttcctcg cacgacactt acgcactatc aaaaaacgct catttacccg
     1141 gcgttacagt aaacgaagca acattctgtg tcctacgctg atgtaaagca cagtgaaaat
     1201 cccccttgtt gacttcgtct gaccttatcg cggcgaccag caattattcg ttttgtgatc
     1261 ctcaacacaa aatgctattc gtcgcctatg aggggagaag aatttatgga aaacgattgc
     1321 attcacaaaa tgacgtcctg tcagaagaga aaaagttgac gttaatcaat tgttcgagat
     1381 gatcaatacc tgtattcgaa attttaaaat attgataaaa taaatgtttg ttctccaaca
     1441 aatagcgtga aaaaatgtga cgtgccgcag tatttatcgc tg
//
LOCUS       DAZXUK010000477         1475 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZXUK010000477 DAZXUK010000000
VERSION     DAZXUK010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.481"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(95..748)
                     /gene="ribB"
                     /locus_tag="ACH3L8_004363"
     CDS             complement(95..748)
                     /gene="ribB"
                     /locus_tag="ACH3L8_004363"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFT4087181.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(852..1000)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1122..1412
                     /gene="ubiK"
                     /locus_tag="ACH3L8_004364"
     CDS             1122..1412
                     /gene="ubiK"
                     /locus_tag="ACH3L8_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFT4087182.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT          407 a          389 c          337 g          342 t
ORIGIN      
        1 gcggaattaa catcttgcat agcagggcaa aataaaaacc gaagccagct acaacttcgg
       61 tttcttgatt aaggcagtaa ttaagcagcg gttttcagct ggctttacgc tcatgtgcct
      121 gacggtatgc caccaggtct tcaatagtca cgagcgccat attgtgttta ttggcaaact
      181 caatgcactc tggtgcacgc gccatcgtgc catcgtcatt agtcagctca cacagcacac
      241 cagccggttt aaagcctgcc agcgtcatca aatcaatagt cgcttcagta tgaccgccac
      301 gcgtcagtac accacctgcc tgcgcgcgaa gtgggaaaac gtggcccggg cgattcagat
      361 cagaaggttt tgcgccatcg gcaatcgctg cgcgaacggt cgtaatacga tcagcggcag
      421 aaacaccggt agtcacacct tcagccgctt caatggtcac ggtaaaaccg gtgccatagg
      481 cgctggtgtt attttctacc atcattggca gatcaagttg tttacggcga tcttcagtga
      541 tgcacaggca aacaataccg ctaccgtggc gaatggtcag cgccatctgc tcaacggtca
      601 tggtttctgc cgggaagatc atatcgcctt cgttttcacg gtcttcatca tcaagcacca
      661 ttacaccgcg tccttcacgc agcgcagcca gtgcattttc aacacgttcg aaaggcgtac
      721 caaaagagga aagtagcgtc tgattcatgg taaaaaaacc tcactaaaat tatggttacc
      781 agaatcaggg cagtcttagg agtggcggca tatagccaaa ataacgtgag cgggtccatg
      841 cccgacagaa tcgttactct ctcccatccg gactctaacc gtcggccccg gaattacacc
      901 ggatctgctg tcctttgagt tcgcacccaa agcgctcgcg ggctttcaac tgagttgatt
      961 taccgccggt ggggaatttc gccccgccct gagaataagc ggattcacta taacgctaat
     1021 gattagcggc agcaacgcat agcttcacat aattctggtt tatgacttac ccttatcgca
     1081 ctacaatggt actcaacacc tatcagtaca gggaaactac aatgattgac ccgaaaaaaa
     1141 ttgagcaaat cgctcgccag gttcacgaat caatgcctaa aggaatcagg gagttcgggg
     1201 aagatgtgga gaaaaaaatc cgccaaaccc tacaagctca gctgacgcgc ctcgaccttg
     1261 taagccgtga agagttcgac gtccaaacgc aagtcctgtt acggactcgt gagaaactgg
     1321 cgctgcttga acaaagaatc agcgaactgg aaaatcgcag tactgagatc aaaaagcagc
     1381 cagatccaga gactctcccc ccaacgctgt aaacatttgt ctttaatgca ctgtaaatta
     1441 tgaattaatc ctctaattta cagtgttttc tatta
//
LOCUS       DAZXUK010000478         1475 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZXUK010000478 DAZXUK010000000
VERSION     DAZXUK010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.482"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            67..1056
                     /gene="ldhA"
                     /locus_tag="ACH3L8_004365"
     CDS             67..1056
                     /gene="ldhA"
                     /locus_tag="ACH3L8_004365"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFT4087183.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1167..>1475
                     /gene="hslJ"
                     /locus_tag="ACH3L8_004366"
     CDS             1167..>1475
                     /gene="hslJ"
                     /locus_tag="ACH3L8_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFT4087184.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT          401 a          341 c          367 g          366 t
ORIGIN      
        1 tatttgaaat tttgtaaaat atttttagta gcttaaatgt gattcaacat cactggagaa
       61 agtcttatga aactcgccgt ttatagcaca aaacagtacg acaagaagta cctgcaacag
      121 gtgaacgagt cctttggctt tgagctggaa ttttttgact ttctgctgac ggaaaaaacc
      181 gctaaaactg ccaatggctg cgaagcggta tgtattttcg taaacgatga cggcagccac
      241 ccggtgctgg aagagctgaa aaagcacggc gttaaatata tcgccctgcg ctgtgccggt
      301 ttcaataacg tcgaccttga tgcggcaaaa gaactggggc tgaaagtagt ccgtgttcca
      361 gcctatgatc cagaggccgt tgctgaacac gccatcggta tgatgatgac gctgaaccgc
      421 cgtattcacc gcgcgtatca gcgtacccgt gacgctaact tctctctgga aggtctgacc
      481 ggctttacta tgtatggcaa aacggcaggc gttatcggta ccggtaaaat cggtgtggcg
      541 atgctgcgca ttctgaaagg ttttggtatg cgtctgctgg cgttcgatcc gtatccaagt
      601 gcagcggcgc tggaactcgg tgtggagtat gtcgatctgc caaccctgtt ctctgaatca
      661 gacgttatct ctctgcactg cccgctgaca ccggaaaact accatctgtt gaacgaagcc
      721 gccttcgatc agatgaaaaa tggcgtgatg atcgtcaata ccagtcgcgg tgcattgatt
      781 gattctcagg cggcaattga agcgctgaaa aatcagaaaa ttggttcgtt gggtatggac
      841 gtctatgaga acgaacgcga tctgttcttt gaagataaat ccaacgacgt gatccaggat
      901 gacgtattcc gtcgcctgtc tgcctgccac aacgtgctgt ttaccgggca ccaggcattc
      961 ctgacagcag aagctctgac cagtatttct cagactacgc tgcaaaactt aagcaatctg
     1021 gaaaaaggtg aaacctgccc gaacgaactg gtttaatctt gcagctcccc tgcaatccag
     1081 gggagctgat tcagataatc cccaatgacc tttcattctc tattcttaaa atagtcctga
     1141 gtcagaaact gtaattgaga accacaatga agaaagtagc cgcgtttgtt gcgctaagcc
     1201 tgctgatggc gggatgtgta agtaatgaca aaattgctgt tacgccagaa cagctacagc
     1261 atcatcgctt tgtgctggaa agcgtaaacg gtaagcccgt gaccagcgat aaaaatccgc
     1321 cagaaatcag ctttggtgaa aaaatgatga tttccggcag catgtgtaac cgctttagcg
     1381 gtgaaggcaa actgtctaat ggtgaactga cagccaaagg gctggcaatg acccgtatga
     1441 tgtgcgctaa cccgcagctt aatgaactcg ataac
//
LOCUS       DAZXUK010000479         1466 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZXUK010000479 DAZXUK010000000
VERSION     DAZXUK010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.483"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            71..352
                     /locus_tag="ACH3L8_004367"
     CDS             71..352
                     /locus_tag="ACH3L8_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFT4087185.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            318..1395
                     /locus_tag="ACH3L8_004368"
                     /pseudo
     CDS             318..1395
                     /locus_tag="ACH3L8_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          506 a          292 c          327 g          341 t
ORIGIN      
        1 tattaaaaat caaccacacc cgccagtatt ctgtatattt actggcggtc atatcgtgag
       61 aggtatggca atgaatcttg tgacactcaa aacgtgggga aaactcagat atccggataa
      121 cccaccatca atatcaacgc tgagacgatg ggcaaggaat ggaaacattt atcctgcacc
      181 tgaactacac gggaggagtt acagggtggt tccggaggct ttctatatca acccaaataa
      241 ggttgatacc gatataacac accatcagcc taatgggcga caagggagag acagtccgtt
      301 aatggagaag ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa
      361 catatcgaag gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta
      421 cactaggtaa aatttcaaga agagatgcag tagcgcaggc aattgaagca aatcattata
      481 tatacaaaaa ctactctcct gctgccctaa ttgaaaagct taaaggggtc gactcattta
      541 ctatggcagg ctggattgaa cgttacaaaa cgattcttat aaggagaaaa gcgtccagaa
      601 atacttataa aactcgggta aatcaactgg agacaataaa agaaaaatta ggaggggttt
      661 tactgacaga aataaccact cgccatatcg ccgagtttct tgatttgtag attgaaggag
      721 ggaaaaacac gatggcagga tcaatgcgtt ctgtgttatc tgatatgttc cgcgaagcca
      781 ttgttgaagg acgtatatct caaaatccag taacgccaac aagagcaccg aaaatagtag
      841 ttacaagaga acggctgaaa ctaaagacat acaactgcat cagggaggca gcagatcaac
      901 ttccggcatg gttcccatta gctatggact tagcccttgt aacaggacaa cgtcgcgaag
      961 acataacgaa tatgcggttc agtgatattt atgatgatcg tctccacatc aggcaaatta
     1021 agacaggaat gatgattgct atccccctgt cactcagcct tcctgtcgct ggtttacgac
     1081 ttggtgcagt agttgaacag tgccgcatgg taagtagggg ggattatcta atcagtgccg
     1141 ggattagaaa aaacagccct gacggcagca ttcacccgga cggcctgaca aagaaatttg
     1201 tcgcagccag aaaattaaca ggtatcactg ttcagtgaaa acccaccaac ttttcacgaa
     1261 atcagaagcc tggctggacg attgtacaaa gaaatatgtg gagaagaatt tgctcagcgt
     1321 ctacttggcc acacatcgga gaagacaaca aaaatgtatc ttgatgagag agaaaaaacg
     1381 tacttactgc tctgatttta acgtaaatgg attgttaaat gtattttggt tgtgatataa
     1441 ccaaaaaaga ccggaataca gaaatt
//
LOCUS       DAZXUK010000480         1451 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZXUK010000480 DAZXUK010000000
VERSION     DAZXUK010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.484"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1008
                     /locus_tag="ACH3L8_004369"
     CDS             <1..1008
                     /locus_tag="ACH3L8_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HFT4087186.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGCLAHARR
                     KIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQSLYDWI
                     QLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGRVEIDNNIGENALRSVAVGRKNYL
                     FFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPWNFSSV
                     K"
     gene            1197..1382
                     /locus_tag="ACH3L8_004370"
                     /pseudo
     CDS             1197..1382
                     /locus_tag="ACH3L8_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          397 a          339 c          422 g          293 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcctggccca cgcccgccga
      481 aaaatccatg acgaggatgt gcgccgtccg acagaaatga ctcaggaagc gctcagacgg
      541 atagcagagt tatacgacat agaagcggag atacgtggca gtccggcaga ggaacggctt
      601 gcagtcagaa aagccagaag cgtccagttg atgcagtcgt tgtacgactg gatacagttg
      661 cagaggaaaa cgctgtcgaa acatgcggag atggcgaagg cgttcgacta tatcctgaat
      721 cactggaatg cgctgaacga gttctgtcgt gacggccggg tggaaataga caacaacatc
      781 ggtgaaaacg cgttacgatc ggtggcggtt ggaagaaaaa attatctctt tttcggctca
      841 gacaagggag gagaaagtgc ggcgatcatc tacagtctgc tggtcacctg caaacagaac
      901 gaagtggagc cggaggactg gttgcgcgaa gtgatcgaga agctcaatga ctggccgtcg
      961 aaccaagtgc atgaactgct gccctggaac ttctcgtctg taaaataatc cttacgctac
     1021 gtacttctcg gggcgcttac catacggtgg tcggtttacg cttaccgcag gacaggtggt
     1081 aactgaaaaa tagtttgctg aggcgactgg aaacagaagg gcaagatcga ttccgacagc
     1141 agtgatggtc agctacctca tcgataactt tgcaacagtg cccttcttga agcatcatgc
     1201 ttcatgaaga aaaactggca cgtcatcaac gtaaacaggc gatgtatacc cgaatggcag
     1261 ccttcccggc ggtgaaaacg ttcgaagagt atgacttcac gttcgccacc ggagcaccgc
     1321 agaagcagct ccagtcgtta cgctcactca gcttcataga gcgcaatgaa aacatagttt
     1381 tacaggggac atctgacatc acaaatcccc gggtggggat ttgtgtataa gagacagccc
     1441 atggaggaag g
//
LOCUS       DAZXUK010000481         1448 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZXUK010000481 DAZXUK010000000
VERSION     DAZXUK010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.485"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1417
                     /gene="ospD3"
                     /locus_tag="ACH3L8_004371"
     CDS             <1..1417
                     /gene="ospD3"
                     /locus_tag="ACH3L8_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFT4087187.1"
                     /translation="NYVSVSEEEKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKS
                     RALEISTSNHNMALGLKIKETKNGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTV
                     DNFLDEKHQKCYGLISDGMSIFVDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTEL
                     IQKVTRVVQLSDLSDNTLELLLAAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLN
                     LDKTVELLTAEGMGGRISGLSQALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAY
                     YYDEVHRQIPGLMFALQNGHADAIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLL
                     LALNNGQADAILAYGDILNEAKLNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAY
                     GKILHTADLTPHQASKLLAAEGPNGVSGLIIAFQNRNFEAIKTYMEIIKNKNITPEEI
                     AEHLDKKNGSDFLEIMKNIKS"
BASE COUNT          516 a          238 c          280 g          414 t
ORIGIN      
        1 aaattatgtt tcagtgagtg aagaagaaaa ggctattaat gtgcctgcta ttatttattt
       61 tgttgaaaac ggttcatggg gagatattat tttttatatt ttcaatgaaa tgatttttca
      121 ttccgaaaaa agcagagcac tagaaataag tacatcaaat cacaatatgg cattaggctt
      181 gaagattaaa gaaactaaaa atggggggga ttttgtcatt cagctttatg atcccaacca
      241 tacagcaact catttacgag cagagtttaa caaatttaac ttagctaaaa taaaaaaact
      301 gactgtagat aattttcttg atgaaaaaca tcagaaatgt tatggtctta tatccgacgg
      361 tatgtctata tttgtggaca gacatactcc aacaagcatg tcctccataa tcagatggcc
      421 tgataattta cttcacccca aagttattta tcacgcgatg cgtatgggat tgactgagct
      481 aatccaaaaa gtaacaagag tcgtacaact atctgacctt tcagacaata cgttagaatt
      541 acttttggca gccaaaaatg acgatggttt gtcaggattg cttttagctt tacaaaatgg
      601 gcattcagat acaatcttag catacggaga actcttggaa acttctggac ttaaccttga
      661 taaaacggta gaactactaa ctgcggaagg aatgggagga cgaatatcgg gtttatccca
      721 agcacttcaa aatgggcatg cagaaactat caaaacatac ggaaggcttc tcaagaagag
      781 agcaataaat atcgaataca ataagctgaa aaatttgctg accgcttatt attatgatga
      841 agtacacaga cagatacccg gactaatgtt tgctcttcaa aatggacatg cagatgctat
      901 acgcgcatac ggtgagctca ttcttagccc ccctctcctc aactcagagg atattgtaaa
      961 tttgctggcc tcaaggagat atgacaatgt tcccggactt ctgttagcat tgaataatgg
     1021 acaggctgat gcaatcttag cttatggtga tatcttgaat gaggcaaaac ttaacttgga
     1081 taaaaaagca gagctgttag aagcgaaaga ttctaatggt ttatctggat tgtttgtagc
     1141 cttgcataat ggatgtgtag aaacaattat tgcttatggg aaaatacttc acactgcaga
     1201 ccttactcca catcaggcat caaaattact ggcagcagaa ggcccaaatg gggtatctgg
     1261 attaattata gcttttcaaa ataggaattt tgaggcaata aaaacttata tggaaataat
     1321 aaaaaataaa aatattacac ctgaagaaat agcagaacac ttggacaaaa aaaatggaag
     1381 tgattttcta gaaattatga agaatataaa aagctgaata ttatctcgtg gtgatacact
     1441 gaatttga
//
LOCUS       DAZXUK010000482         1447 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZXUK010000482 DAZXUK010000000
VERSION     DAZXUK010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.486"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..472
                     /locus_tag="ACH3L8_004372"
     CDS             <1..472
                     /locus_tag="ACH3L8_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4087188.1"
                     /translation="RVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKE
                     SCTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSV
                     LQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            821..>1447
                     /locus_tag="ACH3L8_004373"
     CDS             821..>1447
                     /locus_tag="ACH3L8_004373"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFT4087189.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIY"
BASE COUNT          438 a          278 c          311 g          420 t
ORIGIN      
        1 ccgggtggca tggataaacc cggaagagag taaatcaaac cgcgcaattg gcgttgcgct
       61 gaatgatact gcatgtcgcg tattgaaaaa acaaatcggg aatcatcacc gttgggtatt
      121 tgtgtacaag gaaagctgta ccaaaccaga cggaacgaaa gcgccaacgg tcaggaagat
      181 gcggtatgac gcaaacacag cctggaaagc ggcgctgaga cgggctggta ttgatgattt
      241 cagatttcac gacttgagac acacctgggc aagttggctg gttcaagccg gagtcccgtt
      301 atcagtgtta caggaaatgg gaggctggga gtctatcgaa atggttcgtc gatatgctca
      361 ccttgcacct aatcacctta ccgaacacgc acggcaaata gactcgatcc tgaacccatc
      421 ggtcccaaat tcgtcccagt caaaaaataa ggaaggtact aatgatgtgt aacttattga
      481 tttaaatggt gccgataata ggagtcgaac ctacgacctt cgcattacga atctgtagca
      541 ccaatcataa ctatctgttt tagcaagcat taaccgcatt cactaagcta tagttgatga
      601 cacaaacaga aagttgatgc atgatgttgc catatatatg tcacaaatac ggcacaacga
      661 tcttcaaaca tatagccaca cattcacaga agagcacaaa gccttgcaat ccagtgcaaa
      721 gcttggtgtg tctccgtttt gtttcatcag ttacacataa ctatatgatc attaactaaa
      781 agtcagtata cttggagaaa ttttaattgc gtaatagagc atgcatatga ttgaaattaa
      841 cagtttatta ttaataacat ccgtgatatt gatgtcgtta ttagctgtgg ggttatttga
      901 taaaatttcc ccaataaatc ttgttgagca cggcaggaat aatcaaatag atggaatgcg
      961 ggggttctta gcaattttcg tgcttattca tcacgcagca atttggaatg gatacttgtc
     1021 atctggagta tgggaagcac cttcatcaaa tctgttagca aatttaggcc aagttggtgt
     1081 gtcattcttt tttatgatta ctggttatct gttcttttca aagattatct ctggagatca
     1141 ggactggaca aggctttatg tatcaagatt actacgatta accccaatgt tcatagttag
     1201 cctatgtctc atttttatca ttgtaggttt taagtctgga tggagaatgc aggtatccac
     1261 agaagagctt tttgtgtcaa taatgaagtg gttgccattc actgcgctag gtatgccgaa
     1321 cattaatgac gtaaaagatt catttactat caatgccgct gtaacatgga cacttgtata
     1381 tgaatggttc ttttattttt ctcttccggt aatttccgcg ctcataaaaa gaaaagtcag
     1441 tatttac
//
LOCUS       DAZXUK010000483         1439 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZXUK010000483 DAZXUK010000000
VERSION     DAZXUK010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.487"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..181
                     /locus_tag="ACH3L8_004374"
     CDS             <1..181
                     /locus_tag="ACH3L8_004374"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087190.1"
                     /translation="KIVGDVVVSYSRLMSSGINPLHCEYVKHQKKMNQIYEKSKYTYQ
                     GRKHDITLIPLWQIF"
     gene            247..>1439
                     /gene="ipaHb"
                     /locus_tag="ACH3L8_004375"
     CDS             247..>1439
                     /gene="ipaHb"
                     /locus_tag="ACH3L8_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaHb"
                     /protein_id="HFT4087191.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAV
                     AADAT"
BASE COUNT          445 a          337 c          242 g          415 t
ORIGIN      
        1 taaaatagtt ggagatgttg tagtatctta ctcaagacta atgtcttctg gtataaatcc
       61 tctgcattgt gaatatgtaa aacatcaaaa aaagatgaac caaatatacg aaaaaagcaa
      121 atatacttac cagggtagaa agcatgatat aacactcatt cccttatggc aaatattctg
      181 aaacaccctc ctcattagga gagatacatt aaatacaatt tattcacaat ctaattccat
      241 atccatatgc gagaaattaa tatgctcaga aacatttcat cctgtttatt tccacatatc
      301 agcacaatta catcccccaa ccattatttg tccgaatggg atgattggga gaaacagggg
      361 ttaccggaag aacagcgtac tgaggcggta agaagacttc gtgcatgtct tacctctaag
      421 gggcataaac tggacctgcg agccttggcg ctttcctcgc tacctgtact ccctgcttgc
      481 attaaaaagc ttgatgtgag ctgtaataaa ttaaccatcc ttactgattt acctgaaaat
      541 attaaagaac ttattgcaag agataatttc ttaacacata tatctgcatt accacattat
      601 ctaataactt tggatgtgtc cgaaaatcaa ttagagaatc tgccgttatt accagacacc
      661 atcaaatcac taagcgcaga gtataatagg ttatccacac tgccttcatt acccttgaat
      721 ttaaaaaaac ttgaggttag gaacaacgaa ctgcaaactc ttccatctct gccttctaat
      781 cttaagatac ttaaggttgc gcacaaccat cttactgaac tgcccccttt acctaggaga
      841 ctgcaacttc tttttgcata tagcaataga ttaagcaact taccaaacat ccaagaaaat
      901 attatcatga gaagattttt ttattttgaa aacaaccaaa taactacaat cccgacaaat
      961 ctttttcgtt tagatcctca tataactatt gagattgcaa ataacccctt atcagatcaa
     1021 actctgctat tcttaataca gcaaacttcg gttccaaatt ttaacgggcc tcagtttcgt
     1081 atttccctgt cagaccaaaa cagactgttt ttacgccaga tgttgccgca aaatttacat
     1141 tcgcgccata tcagagtcat cactgaaggg gggcagaact ttcagatccc ccctcttccc
     1201 gaaactgtgg cagcctggtt tcctgaagca gatcgtcggg aggtttctac acaatggact
     1261 tctttttcca ccgaggagaa ttcccgggca ttctccgcgt tccttgaccg cctttccgat
     1321 accgtctctg cacgcaatac ctccggattc cgtgaacagg tcgctgcatg gctggaaaaa
     1381 ctcagtgcct ctgcggagct tcgacagcag tctttcgctg ttgctgctga tgccactga
//
LOCUS       DAZXUK010000484         1432 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZXUK010000484 DAZXUK010000000
VERSION     DAZXUK010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.488"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            29..>1432
                     /gene="gadC"
                     /locus_tag="ACH3L8_004376"
     CDS             29..>1432
                     /gene="gadC"
                     /locus_tag="ACH3L8_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HFT4087192.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDW"
BASE COUNT          356 a          269 c          302 g          505 t
ORIGIN      
        1 tattttaaaa atgttcgaga gtataaaaat gtctaatgct aatatcgcac ccaaaagtaa
       61 gaaaatgaca gtggggacac tcgctatcat gaatataact gctgtagtaa gtctacgatt
      121 tataccttct gaagcagaat acggcttagg tgccattttt tactatacct ttgctgcaat
      181 tatgttttta gtacctgttg cctttgttgc ggctgagcta gctacaacat atccacaaaa
      241 agggggggca tttcgatgga taggagaagc atttggggct cgctggggtt ttgtaggaat
      301 gcttatgacc tggctacaag tcatccccta ctttccaaca gtactgacat ttggtgcagt
      361 ctcggttgca tttattgatg ttgatatggg ggtggcagaa tcaattgcca gtaataaatg
      421 gtatatttta tttttcgtac tatttgttta ctggggagct atttgtgttg cattacgtgg
      481 cgttggtatt ttctcgcagg tatctaagtg gtgcggtatt atagggacaa ttattccagc
      541 tattatcctt gttattcttg gtttctccta tctgtggttc agcggtaaac cagcggtgat
      601 cccattaggt tggggtgatc ttttccctaa tatagctaac ttccagaata ttgttcttgc
      661 cgccagcata tttcttgctt atgcagggat ggaaatgaat gcggtccatg ttaacgattt
      721 agataatcca actaaaaaat acccaattgc aattaccatc gcttctatag gtactattgc
      781 aatattcctg ctcagtactt taggggtcgc ctttattatt cctacagata aaattagtct
      841 aactcaaagt ttgttggttg cctacgatat gctgttcgaa tgggctggag tgccatggct
      901 tggctctgtg atggcattta tgctagcaat tggcgtactg ggtggggtgg ttacttggat
      961 tgcagggcct aatacaggtg tattagcgat cgcgaaagct ggttatttac ctaagttttt
     1021 ccaaaaaacc aatcgtcatg gaatggggca ccatctaatg tttgtacaag gtataattgt
     1081 ctcagttctt tctgttactt ttgtgataat gccttcagtt caagcagctt ttcagatttt
     1141 atcacagtta acagttatgc tctatttagt tatgtatatg ctcatgttcg ctagtgctat
     1201 ctatctacga cattctcaac ctgatacaaa acgcccatat cgagcccctg ctttattttt
     1261 ctgggcaaca cttggattct tagggtcttt tctggctttt gtattaagtt ttattccgcc
     1321 cgagcaaatc ccaactggta gtccaaccag ctatgttctt tatttgttag tattagttgc
     1381 cctattttta ttaattccaa tattaattta cgccttccga aaatctgact gg
//
LOCUS       DAZXUK010000485         1426 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZXUK010000485 DAZXUK010000000
VERSION     DAZXUK010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.489"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            83..449
                     /locus_tag="ACH3L8_004377"
                     /pseudo
     CDS             83..449
                     /locus_tag="ACH3L8_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            452..850
                     /locus_tag="ACH3L8_004378"
                     /pseudo
     CDS             452..850
                     /locus_tag="ACH3L8_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
BASE COUNT          351 a          348 c          419 g          308 t
ORIGIN      
        1 cttgccggtg ttctccgggg taaaaaggtc cacactaccg tcagccggaa agccgttgcc
       61 gcaggcgacc gcgtaaaccg tcatcaaggc aatgtgccgc gtactccggg tggcccgcag
      121 cggctggtat acgtggtgtc agcggcggac aaggataaac acacgtccgc agttccgtca
      181 gcactgcgac agcgttgtcc gcaaggcttt tacccagtcc agcgttacgg tgccccacgc
      241 ctgacggatg aactgtgtgc tctggttacc actttaacgt aaaaaccgtg gcagccagtc
      301 tgcgccgtca ggaactgagc gcaaaagcct cccagaagtt cagcccgatc agttaccgtg
      361 cacacggcct gcctgtatca gaaaatctgc tgacgcagga tttttacgcc agtggcccga
      421 accagaagtg ggcgggagac atcacgtact attacagctc accgactgcg gggaaacacg
      481 gggcgccggg ttattgaagg tgacctgtcc agctacttcg acacagtaca tcaccggctg
      541 ctgatgaaag ccgtctgccg caggatcagt gatacacgtt ttatgagact gctgtggaaa
      601 acgccgtgtt gatgttggtc tcttccgggc ggtcagtgaa agtatgccac agggcggtgt
      661 gatctcgccg ttgctgtcga acatcatact gaatgagttc gaccagtatc tgaataaacg
      721 ctacctgagt ggaaaagcca gaaaggatcg ctggtactgg aatcacagta tccagcgagg
      781 ccgaagtact gctgtcaaag aaaactggca atggaaacct gccgttgctt actgttgcta
      841 tgccgatgac tgtgtgccac gaaggcgcgt cctggggacg tgagtccctg tgcagccatg
      901 cagcacaaga gatgagggaa ggccccttgg tgtcggcgtg caggcgcagg caccaaacca
      961 tccactgctg ctgcatttaa gagatgtggt ggtgagcgag atgggaaaag gtcggacgtg
     1021 agccgatcag gtatggttcg aagagacgaa cgaaagtgaa ccgctgatga tgtgtcgaaa
     1081 gcatatagat gatgtcaaaa caatggtgga gtcgttcctg tgggatatgt ttactgcctg
     1141 cgcggcatcc ggcataaaag cggcgtgaag tcgattcagg ctcttgtggg gaacgtggac
     1201 ctgtcacccc gatgtgaagg gagaagcgca agtggaagcc ccatgagcgc cagagtacca
     1261 atgcggggta cagggacgga gcaccaagcg attgcgcagt aaacatgctt gacaacttaa
     1321 ttggggcccc ctcttccttt tatggtgtaa ttgtttgatt gaatacttcc ggggatttca
     1381 gtatgaaatg aagtatattt aatatacttt acaaaaagtt tgtatt
//
LOCUS       DAZXUK010000486         1412 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZXUK010000486 DAZXUK010000000
VERSION     DAZXUK010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.490"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            8..658
                     /locus_tag="ACH3L8_004379"
     CDS             8..658
                     /locus_tag="ACH3L8_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087193.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            630..1064
                     /locus_tag="ACH3L8_004380"
     CDS             630..1064
                     /locus_tag="ACH3L8_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFT4087194.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
BASE COUNT          399 a          290 c          343 g          380 t
ORIGIN      
        1 gtaatccatg catcgtatag acacgaaaac cgcgaagaag gataagttcg gcgcgggtaa
       61 gaacggtttt acccgtggta acccccagac tggcacgcct gccaccgatc tggatgatga
      121 ctactttgac atgttgcagg aggagctttg cagcgtggtg gaggcatccg gtgccagcct
      181 ggagaagggg cggcacgatc agttgcttac cgcacttcgc gcgctgctgt taagccgcaa
      241 gaatccgttt ggcgatatca aatcggatgg cacggtgaaa acggctctcg aaaaccttgg
      301 tttgggagaa gcagctaaaa ggaatgtagg tacaggggcg aatcagatac ctgatatgag
      361 cctgttcgcg tcaattaata ccgtaacggc tgctgcgcaa aaatttccgt ctggattaat
      421 tttacagtgc ggtcagttga atggtgcccc gaatgtatct tcaacatacg ggatgaggtt
      481 cccgatgacg ttctcaagag taattgctgt cgtagttaca ttgaacgtta ctggcgcggc
      541 tgggcagccg actgtatcgg cgacaagtgt ccagaacact ggatttaata ttactgtgtc
      601 gcccggttca ggatacggtt catctgctga tgcgtattac attgcaatgg gatattaaca
      661 aaatgtcata tttttattct gcatcgacaa acggatttta ttcgactgaa tttcacggca
      721 ccaatattcc tgatgatgca gtggaaatct cggaatcaga gtggaaaaca ctgattaatg
      781 cacagagtgt aacaaaaatg attacctgtg gtgagaacgg tcatcctgtc attgttgacc
      841 gtccttctcc aacaccagaa caattagcct taataaatga tgaaaagaaa tctgcactga
      901 tagcagaggc aacgaatgta atagctccgc ttcaggatgc ggttgattta ggtatggcaa
      961 cagatgatga aacgaaactg ttactggcat ggaaaaaata tcgggtgctg cttatgcgtg
     1021 ttaatgtagt aaaacccgag tggcctatgc atccaaataa atagacttgt taatctagga
     1081 taattgttgg aataatactc gtttttcagt aggttcaggt agcctgggta taatgctcac
     1141 ttctacaatg atttcaactt attttattta attgatatta cgaaacttaa ctcatacata
     1201 aacaatatga ctcaatactg tcctaaatag ctgcgcagaa aagtgctcca ctttcgctca
     1261 gcccttgctg ggcgaagcat caggaaggcg ttctcagcta ggttgtgtta tatctggctt
     1321 gagttttgtt gcccatacgc aacacattac ccatctgtaa acgacaagtg tggcagaatt
     1381 aggtgcagct atttagacta tatcgacgca gt
//
LOCUS       DAZXUK010000487         1409 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZXUK010000487 DAZXUK010000000
VERSION     DAZXUK010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.491"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(64..1160)
                     /locus_tag="ACH3L8_004381"
                     /pseudo
     CDS             complement(64..1160)
                     /locus_tag="ACH3L8_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT          352 a          345 c          340 g          372 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcattttaca gccccgtccg ggctgtatgt tttcaccagc ttatccagtg atcgttttga
      121 aaatggctgc attggatcac gtggctgcaa aaatacataa tccctgttgc actcaggaac
      181 tgaatcacgc caggctttct gctcgtcgat aatccgccgg atctcaggcg ttatcggcag
      241 gcggaaagcc ttttgtgttt tcatagcccc tcgcatgccg ataacccctt ccggataaac
      301 aatttcacca gcctcctcgt aaacgtagtc ccagcgcagg ttatggacat taatcggacg
      361 aacaccagtg atgatcatga agcgaacagc attcttctgg tgtacagagg tgcaggcagc
      421 aacattgagc cagagtcggg cgattgattc aatatcggta aaaagccgtg tgggggtagg
      481 tttctgtacg cgggaggaaa cataatcatc tggcagactg gcggcaacat tgcggccgtt
      541 gcaaagagta ggtgcgcaga acttccagaa ccgacggagc tcggcaaaca actccagggc
      601 gttattgttc gagcgagtgg cgatccactc gtccagcact tccaccagcc gattgtatgt
      661 tacgtcgctg aacacctcac gctcgccgaa cgttacttta atccggtcga tacgcacccc
      721 gtaggttgtg aaactgtccg ggctcagctt ctgccgggct actttggctt tgggtcatcc
      781 cggtacattt ccagcgctgc atgtacggac tctgcccgca gtccaccgtc agccatacct
      841 agtgcttttt cgcgcgccag ctggataacg agctccggcc actcgccgag ctttttacct
      901 ttgaggccca tcttttttgg aaactcggcg taaaatgtaa ccttaccggc tttgctgaaa
      961 tcgatacgga gatagttctc tttttcgtat ttggaacagc gagccacgcc ggaagcagcg
     1021 aggatgattt tggcggcagc aacacagatt ttcatgtgtg cgctggtata ggggggttta
     1081 caggcgtccc atttttcaga tgcggctaaa acatcgtcat tattggggct atccggatta
     1141 tgtgttacag tgcgcggcat tctcaatcct tatctgcgta ggcgcagaaa acaagctcac
     1201 acatacaagt cttttctacg ggacaaaatg caatgtgttg cggttttgtg ttgctggact
     1261 gagtttatca aggttaaata cactggatca acatacagta agttaatgac agtaaagcat
     1321 acaaactcga tacaacttac tgattttaaa atgatttaac ggtaatccat tgaaatgtct
     1381 ttactaatta ctaaacgctg tattaattg
//
LOCUS       DAZXUK010000488         1405 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZXUK010000488 DAZXUK010000000
VERSION     DAZXUK010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.492"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            41..>1405
                     /gene="ccp"
                     /locus_tag="ACH3L8_004382"
     CDS             41..>1405
                     /gene="ccp"
                     /locus_tag="ACH3L8_004382"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFT4087195.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNG"
BASE COUNT          374 a          296 c          387 g          348 t
ORIGIN      
        1 tttctgggtc acaataactc accaccgtga gggaagaata atgaaaatgg tctcacgtat
       61 taccgcgatc ggcctggctg gcgtcgcgat ttgctattta gggttatctg gttatgtgtg
      121 gtaccacgat aataaacgca gtaaacaggc cgatgttcag gcatctgctg tcagtgaaaa
      181 taataaggtt ttaggctttc tccgcgaaaa aggatgcgac tattgccaca cgccttcggc
      241 agaattaccc gcctattatt atattcctgg cgcgaaacag ttgatggatt acgacattaa
      301 gcttggatat aaatctttta acctcgaggc cgtgcgtgcg gcactgctgg ctgataaacc
      361 cgtttcgcaa agcgatctga ataagattga atgggtgatg cagtatgaaa ctatgccacc
      421 aacgcgttat accgcgctac actgggcggg taaggtgagt gatgaagagc gggcggaaat
      481 actggcctgg attgcaaaac agcgcgcgga atattacgcc agcaatgata ctgctccgga
      541 gcatcgcaat gaaccggtgc agcccatccc gcaaaaactg cctaccgatg cgcaaaaagt
      601 ggcgttgggc tttgcgctgt atcacgatcc ccgtttatcg gctgatagca ccatttcatg
      661 cgctcattgc catacgttga atgcgggggg cgtcgatggc agaaaaacat cgattggtgt
      721 tggtggcgca gttgggccga ttaatgcgcc gacggtattt aactcagtat ttaacgttga
      781 gcagttctgg gatggtcgtg cggcaacatt gcaggatcag gcaggtggac cgccgttgaa
      841 cccgattgaa atggcgtcga aatcctggga cgaaattatt gctaagctgg aaaaagatcc
      901 gcagcttaaa gcgcagttcc tcgaagttta tccgcaaggt ttcagtggcg aaaatattac
      961 tgatgccatt gctgaatttg agaaaacatt aattacgccg gattccccat ttgataaatg
     1021 gttgcgtgga gatgaaaatg cgctgacggc gcaacagaaa aaaggctatc aattatttaa
     1081 agataataaa tgtgcaactt gtcatggtgg tattattctc ggcggacgtt cctttgaacc
     1141 gttggggctg aaaaaagact ttaactttgg ggaaattacg gcggcggata ttggtcgtat
     1201 gaatgtgact aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt tacgtaacgt
     1261 tgctttaacg gcaccgtact tccatcgcgg tgacgtgccg acgctggacg gggcggtgaa
     1321 actgatgctg cgctatcagg taggcaaaga gctgccgcag gaggatgtgg atgatatcgt
     1381 agctttcctg cacagtctga acggg
//
LOCUS       DAZXUK010000489         1388 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZXUK010000489 DAZXUK010000000
VERSION     DAZXUK010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.493"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..528)
                     /locus_tag="ACH3L8_004383"
                     /pseudo
     CDS             complement(1..528)
                     /locus_tag="ACH3L8_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(579..1154)
                     /locus_tag="ACH3L8_004384"
                     /pseudo
     CDS             complement(579..1154)
                     /locus_tag="ACH3L8_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1158..1388
                     /locus_tag="ACH3L8_004385"
                     /pseudo
     CDS             1158..1388
                     /locus_tag="ACH3L8_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          303 a          399 c          345 g          341 t
ORIGIN      
        1 tgctgaacat ccccggactt ctgcctcgat ggtatacagt tcaccgatac gctgcagggc
       61 ttccgtggtg atgtcggttg gcactcttgc atgcacatcg tggatttttc tccgggcatg
      121 ggccatacac gcagcttccg ttattctgcc ggattcgtat aacgcccggt aaccaccgta
      181 agcatctgcc tgaagcacac cgctgtaacc ggctaggtga ttctgtggat ggataccttt
      241 ccggtccggg ctgtacgcga accagaccgc cgggggcatc tgtgaaccgg cgttacggtc
      301 atcacggaca tagacccaca gccgggcagt tcttgtttta ccactgcccg gctcctggac
      361 cggaacgggg atatcatccg catgaacttt accgggcatc agtacatact ggcgcaggac
      421 gtcatacagc ggctccagca gttcagcaac ggcaccggtc cagcgaccca gtgtggcacg
      481 gctcagctcc acgccctgac gacggtatat ttctgactgg cggtataact gtcaacgacg
      541 gatgaaaagt ggctcttccg caacctgggt ggagattttc atgccagtgg actcattacc
      601 ctctgcctga gtagttcaaa tcctgctcga ccatacatct ggcgtttcag catcttcttc
      661 aggcgattta catgaccttc gacaacacca ttactccagc gactactgat tgcctcacat
      721 atagctgcag catcagcttc catccccgcc gccacgcgcc gaaattctgc tgaaccgctt
      781 tcgtggactc gagtgaacca actgcttagc tgtgatttat tttgggtttt cagaatacgg
      841 tagaactcga gtgccagttg ctgcgctatt ttcagctcag gttctttttc acacatcaga
      901 ctaataaatc gggaagcata attttcttca tccctggtta ttctccaggg catcaaccag
      961 cggcttaccc gggagactga aggaagtctg acggcagtag taaccggtgg gttccagcct
     1021 ttacgccatt tggcaaccgc atccctgacg atggtttcac tgcctgtaaa tccccgggcc
     1081 accatttccc gccatatccg gctggcgtta taattaccac tttctcgttg ctcttttaac
     1141 cactccctcc atggtgtcag cgaaaaacga aaccgctgtt gaaatccctg gaaagctggt
     1201 tgcggaaaag atgaagaccc agtcgcgaca ctcagagttg gcgaaggcgt tcgcgtacgc
     1261 acttaaccag tggccggcac tgacgtacta tgcgaacgat ggctgggtgg aaatcgacaa
     1321 caacatcgct gaaaatgcct tgcgggcggt cagtctgggt cgtaaaaact tcctgttctt
     1381 cggctctg
//
LOCUS       DAZXUK010000490         1387 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZXUK010000490 DAZXUK010000000
VERSION     DAZXUK010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.494"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..785)
                     /locus_tag="ACH3L8_004386"
     CDS             complement(<1..785)
                     /locus_tag="ACH3L8_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFT4087196.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWV"
     gene            complement(903..1098)
                     /locus_tag="ACH3L8_004387"
                     /pseudo
     CDS             complement(903..1098)
                     /locus_tag="ACH3L8_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
     gene            complement(1169..>1387)
                     /locus_tag="ACH3L8_004388"
     CDS             complement(1169..>1387)
                     /locus_tag="ACH3L8_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFT4087197.1"
                     /translation="SSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITL
                     GVQAGYAHSVSGSSAEGYNGQATLNVTF"
BASE COUNT          300 a          377 c          380 g          330 t
ORIGIN      
        1 acccacaatg cgcggttgag cttaatatcg gtgtcgatgt tgtgaatggc acgggtgtgg
       61 atacgttttc cttttgcact gcggccggaa atcccgcctt tcagcatatt ctcctggatg
      121 gtctgatatg cgctccacag gtctttaccg taatcctccc tgcgtcgtgg tgtcagaatg
      181 tcagcggtgg tgactggctg atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc
      241 agcgcctggc gtgccggtgg cggcaggacc agcgactgca tggcatcacg cttttcctca
      301 atccggtcaa aaacccccac cacttcgtaa gccccttcga taactttctc caccacattt
      361 ccccggtgtg gaacacgcac ttcccccaga gactgaccgc agacgcagcc attctggcag
      421 acgaatctga agtaacccgg cagcatctgg tagctggagg taccgtcatg agagttgagc
      481 agaataattt cagggacatg ttctccgttt atctctccgg cccgccgcag acgcagcatg
      541 tgttttgtgt atccccggcg gcccgggtcg cgcacgcggg tctggcaggc gaagaatggc
      601 tgaaaacctt cctgctgcag gctttccagt acggtgatgg tgggaatgta cgcatatcgt
      661 ttactgcggg aagtatgccg gtcttcaccg aaaatactgg gaacatagtg catcagttct
      721 tcgtgtgtca gcggacggtc acggcgtatc tggttcgcat aaccaaaacg actggctagt
      781 cgcataattt gctccttatc ggtggttacg atttactggt gtaataaatg aaaaagctgc
      841 gtctcccgga gaagacgcgg cctgacagat gaaatgaatg acgtttattg tctgagaagc
      901 ccttaactgg cgagctgagt attaagctgt gttccggcat caccagcgca actgaccttc
      961 agcattacgg ataaccagcc gggaatatgt tccctggtca tcttcagtaa acacattgcg
     1021 gtaagctgtt ttgacggcaa cagcctgttc gcgtgagaaa gggccttcgg gcaataaacg
     1081 tgatgtacaa cccggcatat ctgttgctcc ctgaaagtaa aaagccccgg tcatgatgac
     1141 cggggcctga aggagagtga cctgattatc aaaaagtcac attcagcgtg gcctgaccgt
     1201 tataaccttc agcgctgctg ccgctgacgc tgtgggcata accggcctga acgcccaggg
     1261 tgatattttc ccggacacgg gcttccagtc cggcctgcag gtccagtgac gtgccattcc
     1321 gggacggtga gaacgtcatg ttactgccgg cggcggctgt ccccatgctc atgtcacccc
     1381 gggagct
//
LOCUS       DAZXUK010000491         1382 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZXUK010000491 DAZXUK010000000
VERSION     DAZXUK010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.495"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(393..>1382)
                     /gene="ospC4"
                     /locus_tag="ACH3L8_004389"
     CDS             complement(393..>1382)
                     /gene="ospC4"
                     /locus_tag="ACH3L8_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HFT4087198.1"
                     /translation="IKSFDKIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEK
                     LPLNKTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVV
                     DEVSRYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSL
                     DRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQA
                     LAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVL
                     EYFINNGLVDVNKKFQKVNSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          409 a          262 c          225 g          486 t
ORIGIN      
        1 ttttgacggc ttcggcttta aactccgtag agtaacgttg ggtttttctg ctcattattg
       61 gctccttctg atgccattct atttcaggaa ggagtgtccg ttaaactcag gctacctcac
      121 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
      181 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      241 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      301 ttcactgtca tgctgatgct gcactgtggg gacagaatca ctcatgatga cttcgataat
      361 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      421 gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattgtca
      481 agcatagtgt ccccactatt tactttttga aattttttgt ttacatcaac caatccatta
      541 ttgataaaat attctagcac tttatagttg gcaccatctt tactaagtaa gtattcaaca
      601 tcatacagat tacaatatct attgtttttc attttctcca tctctgcgac atcttgtttt
      661 gtaaaattaa atttagatag taaataaagt gctatatcag cttttttatt gcctaaagaa
      721 taagcaaggg cctgatgagc cattttttta tcagtcactt tattgttaat ctcttcataa
      781 ttagatgctt taatcgcatc ctccaaagat atgtctctga gttttatttt tttgaagtta
      841 tctgtactta gcattcttgg tatatgatac tcaagctgaa acacaaagtt tataattcta
      901 tccagactaa caggatcaat atttttgtta taacaaaact ctctgaatcc ttgatcttta
      961 ctctttctta taaaatcaat aagatgcaac ccaattccaa gcctcatatc tttagtgtta
     1021 aatattggaa catcagtttt cccctgatta ccatgcacat acctgctaac ttcatccaca
     1081 acctctttaa agttatctaa gaaaaatgat tggtgctcat ggtaaaaaac aggtggaata
     1141 gcattatcaa agtgatcggt taatgtcata tatccatatg gagagtcatg atcaatgata
     1201 tacgcattgg cgccaaaatc tacagtatga tgtgttttat ttagtgggag tttttcttga
     1261 tgattggaaa tttcgacccc aaaaaaaaca aaatcgtggt tagagatttc tttaatatcc
     1321 tcagagtatg tattcttatg tgtttgtata ttttcagact gaattttatc aaaggattta
     1381 at
//
LOCUS       DAZXUK010000492         1363 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZXUK010000492 DAZXUK010000000
VERSION     DAZXUK010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.496"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..882)
                     /gene="ipaH3"
                     /locus_tag="ACH3L8_004390"
     CDS             complement(<1..882)
                     /gene="ipaH3"
                     /locus_tag="ACH3L8_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFT4087199.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLSQQTAQPDYHGPRIYFSMSDGQQNTL
                     HRPLADAVTAWFPENKQS"
BASE COUNT          386 a          232 c          339 g          406 t
ORIGIN      
        1 agattgtttg ttttccggga accatgctgt cacggcatca gccagggggc gatggagtgt
       61 attctgttgt ccgtcactca tggagaagta aatccgtggg ccgtggtagt ccggttgggc
      121 ggtttgttgc gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat
      181 agtgcaggtc ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg
      241 gcaccggaaa aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag
      301 gctttccaga agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag
      361 aaatgtcagc tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag
      421 catggttagc tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag
      481 catggttagt tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag
      541 tgttgacagg cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa
      601 tgatattagg gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg
      661 tagcgaggac agatttaaac gattcagttg aagctcactg aactgattga tgagacattc
      721 tttaagtagg gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt
      781 atcccatgct gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg
      841 ggacaatgaa aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga
      901 caaatggctt tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga
      961 acttcagcac ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg
     1021 tttcagttaa ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa
     1081 acatcgtact tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc
     1141 aaccccaaat cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct
     1201 gatggatgtt cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt
     1261 gctacggaat tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc
     1321 ccgaaaattt ctctaaaaaa ctcgaaaaaa atggtaactg gtt
//
LOCUS       DAZXUK010000493         1356 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZXUK010000493 DAZXUK010000000
VERSION     DAZXUK010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.497"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            390..1139
                     /gene="phoC"
                     /locus_tag="ACH3L8_004391"
     CDS             390..1139
                     /gene="phoC"
                     /locus_tag="ACH3L8_004391"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase PhoC"
                     /protein_id="HFT4087200.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1194..1298)
                     /locus_tag="ACH3L8_004392"
                     /pseudo
     CDS             complement(1194..1298)
                     /locus_tag="ACH3L8_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          383 a          275 c          273 g          425 t
ORIGIN      
        1 ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa ggagtgtccg
       61 ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat atgttctact
      121 aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt ccccttgttg
      181 gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct ttttatgctt
      241 gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt tatttaaatt
      301 atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa aataattata
      361 aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact cttagtattg
      421 tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat gtgacaacaa
      481 aacctgacct ttactacctg acaaacgata atgctattga cagtctggca ttattaccgc
      541 ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat gaaaaggggc
      601 gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat ctcagtagtg
      661 gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct aaagattcac
      721 cagaattgca taaattactg acaaatatga ttgaggatgc cggagaccta gcaacgcgtt
      781 cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt tctacgtgta
      841 atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt catacatcga
      901 ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag gataccattc
      961 tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac tggcaaagtg
     1021 atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac tcaaatcctg
     1081 tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag aaaaaataat
     1141 gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt cagttaagca
     1201 actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt taccactctc
     1261 ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt aattacgtaa
     1321 gcgttgataa aagagtcgga tgcagcccag tttcac
//
LOCUS       DAZXUK010000494         1342 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZXUK010000494 DAZXUK010000000
VERSION     DAZXUK010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.498"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..207
                     /locus_tag="ACH3L8_004393"
     CDS             <1..207
                     /locus_tag="ACH3L8_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087201.1"
                     /translation="QKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQ
                     IYRQLTDEVLALRLPENGSQLHHS"
     gene            complement(387..1010)
                     /locus_tag="ACH3L8_004394"
     CDS             complement(387..1010)
                     /locus_tag="ACH3L8_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFT4087202.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RTEQEAKPFTFEWNGRTWNADASSVARLSPVVMLAKSVAAQTHMVWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSSLDDLASIRAFDVK"
     gene            complement(961..1342)
                     /locus_tag="ACH3L8_004395"
                     /pseudo
     CDS             complement(961..1342)
                     /locus_tag="ACH3L8_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          295 a          395 c          333 g          319 t
ORIGIN      
        1 cagaaatatg agatgctgga gaatgagtac cctcagaggg tggctgaccg gctgaaagca
       61 tcaggtctga gcggtgatgc ggatgcggag agggaagccg gtgcacaggt gatgcgtgag
      121 actgaacagc agatttaccg tcagctgact gacgaggtac tggccctgcg attgcctgaa
      181 aacggctcac aactgcacca ttcataatca catcgcataa accacagacc ggactgactc
      241 cggaaaaaca gaggcccgcc cccgggcctc cccggattca tccgtttctc tgttcagcct
      301 gaccgcacac cccggcggcc ggatgacaga ctccgcctcg gtaagcaaag cggtcttctg
      361 tgattccgcc agttgcggct tattcattac ttaacgtcaa acgcccgaat tgaagccaaa
      421 tcatccagac tgctcagctc ctctttcatc tcccgctgac ggcgataaat ctcatcgttg
      481 cgatcgacct gcgcctgcac cattgctgcc gccagttctt ccagttccgg catcgacagt
      541 ttcacctgct gattatccgc atcactccac accatatgtg tttgtgccgc gacagatttt
      601 gccagcatga ccaccgggga caggcgggcc actgacgagg cgtcagcgtt ccacgtgcgg
      661 ccgttccatt caaacgtgaa cggcttcgcc tcctgttctg tgcgccatgc ttcaatttcc
      721 tgacgtctgg cctctctggc cgcttccagc atttctggtg tcacagtgaa tggggctatc
      781 tcaccccatt tgccactttg cagttcctgc cagatttgct gacccgtcgg tgcaacatca
      841 tcagcggtgg ctgtgtaggg aactgcctgg tccctgtcgt caaaaaaaac gtcacagtct
      901 actgcgccac tttcggtata acggggatta atgatttttt taatttccac ggtgcattcc
      961 tcacgatgtg cgaataaaaa gcccgggcat tgcgccagag acatgtgcat ccggcacccc
     1021 ggacagggcg caatatgacc ccggtaatga atgctctgaa catcccgtaa tgaaaaattg
     1081 tggggatgct atatacgttc cggtgggagt accgggcact gaaatcccca ccggtcccag
     1141 tcgtgagcct ctgtatgact gccccctgac aagtctgatg actttatcac cgtcagcttc
     1201 tccctggtac gcagcaataa tcagcccgcc aatgtcaggg tctccccatc tgttgcggac
     1261 agagctcgcc acgattctgt aaataatatc ttctgtggtt atatttattt tcacccagtc
     1321 agtctggata tgggccagta gc
//
LOCUS       DAZXUK010000495         1330 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZXUK010000495 DAZXUK010000000
VERSION     DAZXUK010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.499"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(41..100)
                     /locus_tag="ACH3L8_004396"
     CDS             complement(41..100)
                     /locus_tag="ACH3L8_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFT4087203.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1265)
                     /locus_tag="ACH3L8_004397"
                     /pseudo
     CDS             complement(339..1265)
                     /locus_tag="ACH3L8_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
BASE COUNT          266 a          354 c          321 g          389 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca
//
LOCUS       DAZXUK010000496         1328 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZXUK010000496 DAZXUK010000000
VERSION     DAZXUK010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.500"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            421..590
                     /locus_tag="ACH3L8_004398"
                     /pseudo
     CDS             421..590
                     /locus_tag="ACH3L8_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            736..>1328
                     /locus_tag="ACH3L8_004399"
     CDS             736..>1328
                     /locus_tag="ACH3L8_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFT4087204.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRG"
BASE COUNT          321 a          308 c          410 g          289 t
ORIGIN      
        1 aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca tcccaaaccc
       61 ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg gagattatct
      121 ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc tgctggcctg
      181 gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg gcggattgat
      241 atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg aaattctccg
      301 cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt cttgcggcgc
      361 agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc tgttcgccag
      421 aaaaaatttg acccgttgtc agttgatctg cccgactggt tgtcagaaac actctggcat
      481 gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct gggcgctaac
      541 ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga gcaggtgatt
      601 cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt tcggcctgag
      661 acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc gccaggcttc
      721 agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg tatccgcaga
      781 ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg cgagtggcaa
      841 cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca gttgcgggtg
      901 gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc gtttgcgaat
      961 taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc catagctgac
     1021 gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac cggaaaaaat
     1081 catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt gattatcgtc
     1141 accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa atccggtgaa
     1201 aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat tggcatgcag
     1261 cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg gacggcatcg
     1321 ttacgcgg
//
LOCUS       DAZXUK010000497         1325 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZXUK010000497 DAZXUK010000000
VERSION     DAZXUK010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.501"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..691
                     /gene="spa32"
                     /locus_tag="ACH3L8_004400"
     CDS             <1..691
                     /gene="spa32"
                     /locus_tag="ACH3L8_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HFT4087205.1"
                     /translation="KGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQDIKAR
                     EKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHSLSIDK
                     NIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSEMTYHF
                     KQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKGNKNNS
                     TGYNEQSEEEC"
     gene            685..>1325
                     /gene="spa33"
                     /locus_tag="ACH3L8_004401"
                     /gene_synonym="spaO"
     CDS             685..>1325
                     /gene="spa33"
                     /locus_tag="ACH3L8_004401"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFT4087206.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEV"
BASE COUNT          470 a          184 c          245 g          426 t
ORIGIN      
        1 taaaggagaa atttatagtg gcattaaaaa tgcgttcgaa cttagaaacg aacgagcgac
       61 atattctgat attccagaat caatggccat taaagaaaat attttgatac cagatcaaga
      121 tatcaaagca agggaaaaaa taaatatcgg cgatatgagg gggatctttt catataataa
      181 gagtggaaat gcagacaaga acttcgaaag aagtcatact tcttctgtaa accctgataa
      241 tctgctagaa tctgataata gaaatggtca aattggttta aaaaatcata gcttgtctat
      301 tgataagaat attgctgaca tcatttcttt actaaatgga agtgttgcta aatcatttga
      361 gctgcctgta atgaataaaa atactgcaga cataacccca tccatgtcat tgcaagaaaa
      421 atcaatagtt gaaaatgata aaaatgtttt tcaaaaaaat agtgaaatga cttaccactt
      481 taaacagtgg ggggctggac attctgttag tatttcagtg gagtctggtt cttttgttct
      541 aaaaccgtca gatcaatttg taggaaataa acttgactta attttgaaac aagatgctga
      601 gggtaattac agatttgata gcagtcaaca taataagggg aataaaaata atagtacagg
      661 atataatgaa cagagtgaag aagaatgcta agaattaaac attttgacgc taacgaaaaa
      721 ctacagattt tatatgcaaa gcaactctgc gagcgttttt cgattcagac attcaaaaat
      781 aaatttacag gcagtgaaag tttagtcact cttacttccg tgtgtgggga ttgggtaatt
      841 cgtattgata cattatcttt tttgaaaaaa aaatacgagg tattttcagg attttctaca
      901 caagaatctt tactgcattt atcaaaatgt gtctttatag agtcgtcatc tgtattttcg
      961 attccagaac tgtctgataa gattactttc cggatcacga atgaaatcca gtatgcaact
     1021 actgggagtc atttatgctg tttttcctct tctttaggta ttatttattt tgacaagatg
     1081 ccggtattac gtaatcaagt ttctcttgac tcattgcatc atcttttaga gttttgctta
     1141 ggttcatcta atgtaaggct ggctacttta aaaagaattc gcactggtga tataatcata
     1201 gttcagaaac tttataattt attattgtgt aatcaagtta ttattgggga ttatattgtg
     1261 aatgataata atgaggcaaa aattaatctg tcagaaagta atggtgagtc agaacacaca
     1321 gaagt
//
LOCUS       DAZXUK010000498         1314 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZXUK010000498 DAZXUK010000000
VERSION     DAZXUK010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.502"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            134..1285
                     /locus_tag="ACH3L8_004402"
                     /pseudo
     CDS             134..1285
                     /locus_tag="ACH3L8_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
BASE COUNT          326 a          333 c          367 g          288 t
ORIGIN      
        1 tgctactaat ggtattactg cgcttacaga tgatatatca gaaagcgccc gttttagttt
       61 taatggaggg tgatcttacc caccaatagt ggacacagga ctaagtgagt aaactctcaa
      121 ccagaggtga ctcatgacaa aaccagtatc aatcagcaag aagccccgta aacaacatac
      181 gcctgaattt cgtaacgaag ccctgaaact cgctgaacgc atcggtgtgg ccgccgcagc
      241 ccgtgaactc agcctgtatg aatctcagct ttatgcctgg cgcagtaaac agcagcaaca
      301 aatgagttcg tcagagcgcg aaagcgaact ggccgctgaa aatgtccgcc ttaaacgaca
      361 actggcggag caggctgagg aactgtccat cctccaaaag gccgcgacat acttcgcgaa
      421 gcgcctgaaa tgaagtatgt ctttatcgaa aatcatcggg cagagttcag catcaaagcg
      481 atgtgtcgtg tacttcgggt tgcccgcagc ggctggtatg tctggctcag gcgtcgtcac
      541 cagatgagcc tgcgccaaca gtttcggctc acctgcgatg ccgctgtcca taaggcattc
      601 tttgaggcaa agcagcgata cggtgctccc cgccttgctg acgaactgcc ggagttcaat
      661 attaaaacca ttgccgccag cctgcgtcgt caggggctgc gggcgaaagc cagccggaag
      721 ttcagcccgg tcagctaccg tgcacatggc ctgcccgtat tggagaatct gctggagcag
      781 gacttcagcg ccagtggccc gaaaccagaa gtgggcgggt gacatcacgt acttgcgtac
      841 cgatgagggc tggttgtatc tcgcagtagt catcgacctg tggtcacgcg ccgtcattgg
      901 ctggtcgatg tcaccgcgaa tgacagcaca actggcctgt gatgcactgc aaatggcgtt
      961 gtggcggaga agacgcccgg aaagcgtcat tgttcatacg gaccgtggtg gtcaatactg
     1021 ttcaggggat tatcaggcaa tgctgaagcg acacaacctg cgtggcagta tgagtgcgaa
     1081 aggctactgt tatgacaatg cctgtgtgga aagcttcttt cattcgctga aggtggaatg
     1141 tatccacggg gaacgcttta gcagccggga aataatgcgg gcaacggtgt ttaattatat
     1201 cgagtgtgat tacaatcgct ggcgtcgtca cagtgcctgt ggcggtctca gcccggaaca
     1261 atttgaaaac cataatctcg cttagggccg tgtccacatt acgtgggtag gatc
//
LOCUS       DAZXUK010000499         1309 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZXUK010000499 DAZXUK010000000
VERSION     DAZXUK010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.503"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..910
                     /gene="ldtC"
                     /locus_tag="ACH3L8_004403"
     CDS             <1..910
                     /gene="ldtC"
                     /locus_tag="ACH3L8_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFT4087207.1"
                     /translation="AVALALPAKANTWPLPPASSRLVGENKFHVVENDGGSLEAIAKK
                     YNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPREGIVINIAELRLYYYPPGK
                     NSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTANIRARYKAQGIELPAVVPA
                     GPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCIRLRDDDIKTLFSQVTPGT
                     KVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPITLNSAMQSFKDAAQTDAE
                     VMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(993..1205)
                     /locus_tag="ACH3L8_004404"
     CDS             complement(993..1205)
                     /locus_tag="ACH3L8_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFT4087208.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT          334 a          334 c          334 g          307 t
ORIGIN      
        1 tgccgtggcg ctggcgctac cggcaaaagc caacacctgg ccgctgccgc cagcgagcag
       61 tcgtctggtt ggcgaaaaca aatttcatgt ggtggaaaat gacggtggtt ctctggaagc
      121 catcgccaaa aaatataacg tcggctttct cgctctgtta caagctaacc ccggcgttga
      181 tccttacgta ccgcgcgcgg gcagcgtgtt aacgatcccg ttgcaaaccc tacttccaga
      241 cgcgccgcgc gaaggcattg tgatcaacat tgcggagctg cgtctctatt actacccgcc
      301 gggtaaaaat tcggtaaccg tgtatccaat aggtattggt cagttaggtg gtgacacgct
      361 gacaccgaca atggtgacca ccgtttcaga caaacgtgca aacccaacct ggacgccaac
      421 ggcaaacatc cgcgcccgtt ataaagcaca gggaattgag ttgcctgcgg tagtgccggc
      481 tggaccggat aacccaatgg gccatcatgc gattcgtctg gcggcctatg gcggcgttta
      541 tttgcttcac ggtacgaacg ccgatttcgg cattggcatg cgggtaagtt ctggctgtat
      601 tcgtctgcgg gatgacgata tcaaaacact ctttagccag gtcaccccag gcaccaaagt
      661 gaatatcatc aacactccga taaaagtctc tgccgaacca aacggtgcgc gtctggttga
      721 agtacatcag ccgctgtcag agaagattga tgacgatccg cagctgctgc caattacgct
      781 gaatagcgca atgcaatcat ttaaagatgc agcacaaact gacgctgaag tgatgcaaca
      841 tgtgatggat gtccgttccg ggatgccggt ggatgtccgc cgtcatcaag tgagcccaca
      901 aactctgtaa atcctggata aaaaaaatcc ccgcgacacg gcatgtcgcg gggaaaaaat
      961 cagcagtagc aggcattaat gagggttaat gcttatttat aaattactgc tgttccatgg
     1021 agggtattcg gaccggttac agaagtaata cggaaagatt ttgcgcccat ctcatccgct
     1081 ttttgcgcca gctgctcttc cagcgatccc agatttgtcc ccgcgttagc actgatagta
     1141 ccgacttttt gttggccttc tggcgttgac tgaacttcga cagccgcaaa gttggcaaat
     1201 gacatggagc ttaaaatcgc cgcagcgatg aggtttttac gtttttcata atagtggcct
     1261 tatgcagatg aatgacgtcg caagaaatta cttaacgatc gataactaa
//
LOCUS       DAZXUK010000500         1306 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZXUK010000500 DAZXUK010000000
VERSION     DAZXUK010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.504"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1201
                     /locus_tag="ACH3L8_004405"
     CDS             <1..1201
                     /locus_tag="ACH3L8_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFT4087209.1"
                     /translation="IKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDF
                     FDDFYWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFI
                     LMEKYKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQET
                     ILTLLAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLP
                     GLFFALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGD
                     ILKILPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVE
                     MLSATNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEII
                     NRFNHFL"
BASE COUNT          460 a          206 c          244 g          396 t
ORIGIN      
        1 aataaagcgc gtacaagaaa gtgaaaaatg ggttgttcag tttttcgatc ctaatcgaac
       61 tgttactcat aaacgtactg tatttacttg tgatagccat ttcgaactga gtcaattatc
      121 cgctaaggat ttttttgatg atttttattg gaaaatttat ggtctggagc aacccgggca
      181 ggtaatattt gaagataggc ataattcccc tctaacaaac acagtgaaac ttttaccaga
      241 tgaattgatc aattccagag ttatatatca tgcgataaca aagaatctga cagaagtatt
      301 atttatactg atggaaaaat acaaaaatgg tgaaatttct caatctaaac tggtgaacct
      361 tctggccacg agaagctctg atggtactcc tgcattttat atagcacttc aaaacggata
      421 ttcagacatt attcaagtat atggtaagat attaaatatg tgcaatctta gtcaagaaac
      481 aatacttaca ttgcttgctg cggtaggggc aaacaatgtg ccgggtttat gcatgtcttt
      541 tatgaacgga catgtagaca caataaaagc ctatggggaa atagtcttta aaaccccgtt
      601 aacttccgat aagagattat atctattagc tgcgaaagac agccatgatt tacctgggtt
      661 attttttgca ttgcagaatg gacatgcaga tagtattcgt atgttcggaa gtctgcttaa
      721 taagaaaatg cttagtagtg aacaaattaa agaattattg aaagtaaagc atgggttgtt
      781 tatggcactt caaaatgggc acacaaaggc cattatggcg tatggagata tactaaaaat
      841 actcccccct caccaggaat atatagatga attattatgg ataaaaaatc ccaacggtac
      901 atcaggactt tttatggcat tttataatgg ccataccgaa acaatacgtg ccttttgtaa
      961 tattctaaaa aactatagtt ttaccacaag acgattggtc gaaatgctgt ccgcaactaa
     1021 taaagatggt attcctggtg tatttgtatc tgtagtaaat agagacaaag aaacaatatt
     1081 agagtattgt agaataataa aagagaacaa tctggaaccg gatactatag cagaacaatt
     1141 ttcaaagaag atgaaaaaaa cgtttattga aataataaat agatttaatc attttttatg
     1201 agactgtaat ttaaattgtg taattacctg tttttgatat gttcactcca acaaggtgta
     1261 gtgaccgggt aactgccaac tttcaggcaa ccacctcaat tggcaa
//
LOCUS       DAZXUK010000501         1304 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZXUK010000501 DAZXUK010000000
VERSION     DAZXUK010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.505"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            171..>1304
                     /gene="pdeF"
                     /locus_tag="ACH3L8_004406"
     CDS             171..>1304
                     /gene="pdeF"
                     /locus_tag="ACH3L8_004406"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFT4087210.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT          299 a          284 c          288 g          433 t
ORIGIN      
        1 taataattca ttaatcaggg ttattttgtt tgatatatat caattggaac tttcatatga
       61 cacttagaat caatgctctc tatagtgatg aataatcatc attcgaagtc aggtgggatg
      121 cctgtctgaa tacacctcct tcaggatgtg ggggattctg ctgagcatct atgaaactga
      181 atgcaactta tataaaaata cgtgataaat ggtgggggct tccgctgttc ctgccttctt
      241 taatcttgcc cattttcgcc cacattaata ctttcgcgca tatttcttcc ggtgaggttt
      301 ttctctttta tctgcctttg gcactgatga tcagcatgat gatgtttttc agctgggcgg
      361 cattgccagg gatcacctta gggatttttg tccgcaaata tgcagagctg ggtttttacg
      421 aaacgctctc attaacggct aattttatta tcattatcat tctctgttgg ggcggttaca
      481 gggtctttac tccccggcgt aacaacgttt cacatggtga tacccgttta atttcccagc
      541 gtatattctg gcagatagtg tttcctgcaa cgctgtttct gatacttttc cagtttgctg
      601 catttgtagg attactggcg agcagagaaa atctggtcgg tgtcatgcct tttaacctcg
      661 ggactttaat caattatcag gccttactgg tgggtaatct gatcggtgtc ccgctgtgct
      721 acttcatcat tcgggtagtg cgaaatccat tttatttacg tagctattat tcgcaattaa
      781 aacagcaggt tgatgccaaa gtcaccaaaa aagagttcgc gctctggcta ctggcattag
      841 gtgctttatt gttgctgtta tgcatgccgt taaatgaaaa aagcacaatt tttagcacca
      901 attatacctt gtcattattg ctgcccctga tgatgtgggg agcaatgcgc tatggttata
      961 agctgatttc gctgctctgg gcggtcgtgt tgatgatcag catccacagc tatcaaaatt
     1021 acattcccat ttatcctggc tataccacgc agctgaccat aacctcctcc agttatctgg
     1081 tattctcttt tattgtcaat tatatggctg tactggcaac tcgtcagcga gcggtagtca
     1141 gacgcattca gcggcttgcg tatgtggacc cggtggttca tctgccaaat gttcgcgccc
     1201 tgaatcgcgc gttgcgtgat gccccctggt ctgcgctttg ttatttacgc atccctggca
     1261 tggaaatgct ggttaagaac tatggcatca tgctgcggat tcaa
//
LOCUS       DAZXUK010000502         1301 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZXUK010000502 DAZXUK010000000
VERSION     DAZXUK010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.506"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            45..449
                     /gene="stpA"
                     /locus_tag="ACH3L8_004407"
     CDS             45..449
                     /gene="stpA"
                     /locus_tag="ACH3L8_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFT4087211.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(496..1020)
                     /gene="ygaP"
                     /locus_tag="ACH3L8_004408"
     CDS             complement(496..1020)
                     /gene="ygaP"
                     /locus_tag="ACH3L8_004408"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFT4087212.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1030..>1301)
                     /locus_tag="ACH3L8_004409"
     CDS             complement(1030..>1301)
                     /locus_tag="ACH3L8_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFT4087213.1"
                     /translation="TEQAAALLKAMSHPKRLLILCMLSGSPGTSAGELTRITGLSASA
                     TSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          341 a          312 c          316 g          332 t
ORIGIN      
        1 gacgaaatac ttttttgttt tggcgttaaa aggttttctt tattatgtcc gtaatgttac
       61 aaagtttaaa taacattcgc accctccgtg cgatggctcg cgaattctcc attgacgttc
      121 ttgaagaaat gctcgaaaaa ttcagggttg tcactaaaga aagacgtgaa gaagaagaac
      181 agcagcagcg tgaactggct gagcgtcagg aaaaaattag cacctggctg gagctgatga
      241 aagctgacgg aattaacccg gaagagttat tgggtaatag ctctgctgct gcaccacgtg
      301 ctggtaaaaa acgccagccg cgtccggcga aatataaatt cactgatgtt aacggtgaaa
      361 ctaaaacctg gaccggtcag ggccgtacac cgaagccaat tgctcaggcg ctggcagaag
      421 gtaaatctct cgacgatttc ctgatctaat tactgaggcc ggatgtcgct gctagggcgc
      481 gtccggcgtc gcttctcaag ctcgttggtt ccacggcatc ttatctaaca accttgccat
      541 tccacaaaaa ccgctgattc ctgcaaacag taacccggca ccaacaaagc cacttaataa
      601 gaagaaaccg ctatttacgg tataacccag tacaacgccg tttaatatta aaccacccgc
      661 agcgatctgc acctggcgca ttaacggcaa gggttgagat ttatttaccg ctactggcaa
      721 tccagctctt ttccagccat caatcccatc ttcgagtaaa aatatttctg cgggggcggc
      781 aatcgctgct aatttatcgg cgttattact ggtgcgttta cctgcctggc agtggaatat
      841 aatttgctcg cgacgtaatt taggtggaag acctgactgt tccagcacgg ataatggagc
      901 cagatctgct tcaggaatat gttcacgaag atattcatca gcgtcacgaa tatcgattaa
      961 ctttgcgccg cgtgcgatta attcttgtgc atcatgcggc gaaatggttg tcaaagccat
     1021 agtgactcct tacggacaat agacattttt cagggtggcg ataatggcat ttaccgcctc
     1081 atttttaatg gaatatagaa tgcgttgggc atcccgttgg ctgttgataa gcccttcgtc
     1141 ccgcatacga gcgagatgct gtgatgtcgc agaggcgctc agtccggtaa tgcgcgtcag
     1201 ctctcccgcg ctggtgccgg gggaaccgct aagcatgcac agaatcagca accgtttagg
     1261 gtggctcatt gctttcaata aggccgctgc ctgttcggta c
//
LOCUS       DAZXUK010000503         1299 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZXUK010000503 DAZXUK010000000
VERSION     DAZXUK010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.507"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            10..1299
                     /locus_tag="ACH3L8_004410"
                     /pseudo
     CDS             10..1299
                     /locus_tag="ACH3L8_004410"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          365 a          233 c          332 g          369 t
ORIGIN      
        1 attttgataa tggccttact tgcgggtcag ttaagtgccc aggagtggtc atttgattct
       61 tcacagttgg aaggaaacgt tagtgcggac actgtggcaa tgtttaatca gggagagcaa
      121 ctccccggaa attatcgtgt ggaaatttat cttaacggtg aaaaagttga tgttggggag
      181 tttccctttc atcgtccgga atcccctgaa gaaaaagagc ttgttccatg tttaaccgtt
      241 gatgacctta tccactatgg cattaaaatt gataaaagca gcagtgatac tgacaataaa
      301 aagaatcagt gttttaaatg gaactctatt gaaggtttga aagtaaatta tgattttgat
      361 agccagcgcg tacagataac cgttccacaa ctgtatctgc aagataaaaa aagtagttta
      421 gcaccagtgt cgttatggaa tgagggtgta gctgcattcc gcatggtgta tcagacaaat
      481 attgatattt ccaaacaaaa tgataatcaa tctaccacgc gtaattctcg ttatggcagg
      541 ttcactccag ggtttaatct tggtgcctgg aggtttcgta gttcagtgac atggagcaag
      601 gaacttggcc agtcagaaag atggcaaagg ggttacatgt ggtttgagcg aggcataaat
      661 gcgattaaaa gccgattaac gcttggagaa tcttatacgt cgtctgaggt gtttgacagc
      721 ataccttttc gcggcgggat gttagcgacc gacgatgcga tgactccgcc ggaggacagc
      781 tactacacac ctgtggtgca tggaatagca caaagtgagg cgcaggttat tattaaacaa
      841 aatgggcaaa ttatttttac ccgttctgtg ccgccagggc cttttgcgct ggataattta
      901 ccaacgctgg ccgttggcgg ggagcttgat gttacagtga gagagagtaa tggcgaggag
      961 cagtattttt ccgtgccatt tcaaacgccg gccatcgctc tgcatgaagg atatttcaaa
     1021 tatagtgtga tgggggggaa tataaagaag aaggtataaa aagctcccct gtctccgtgg
     1081 gcgaaatcac ggcgatgtac gggcttcctt atggcgtcac ggtgtatgga gggattcaaa
     1141 acgccactca ttttaatgca atatctacgg gtattggcat ttctctgggg ctgttaggga
     1201 gtttatcgac agatattacg cgttcgattg cgaatttata ttatgggaat aaatatcgta
     1261 ttcgttatag taaatccatt agtgattttg gtactcaat
//
LOCUS       DAZXUK010000504         1287 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZXUK010000504 DAZXUK010000000
VERSION     DAZXUK010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.508"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            134..853
                     /locus_tag="ACH3L8_004411"
     CDS             134..853
                     /locus_tag="ACH3L8_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFT4087214.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          381 a          243 c          256 g          407 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgttcggag aggaccctta cgcttacata cattacatat
       61 aatgtatcgt tttataaaag atgataaaca atcaatataa gataatatat ctattttata
      121 gaggacgttt tctatgccca taaaaaagcc ctgtctaaaa ctaaatttag attctttgaa
      181 tgttgtgagg tctgaaatac cgcagatgct ttctgcgaat gaaagattga aaaataactt
      241 caatatattg tacaaccaaa tacggcaata cccagcgtac tatttcaaag tagcatcgaa
      301 cgtgccaact tactctgata tatgtcaatc tttttctgtc atgtaccaag gattccagat
      361 tgtgaaccat agtggagatg tgtttattca tgcatgtcgt gaaaatcctc agagtaaggg
      421 ggattttgtt ggggacaagt ttcatattag tatagctagg gagcaagttc ctctggcatt
      481 ccaaattttg tctggtttat tattttcaga ggacagtcct atagataaat ggaagataac
      541 tgatatgaat cgagtttctc agcaatctcg tgtggggata ggagctcagt ttacgctata
      601 tgtaaaatca gatcaggaat gctcgcaata tagtgcttta ttacttcata aaatacgaca
      661 atttataatg tgtctcgagt ctaatctatt aagaagcaaa atcgctcccg gggagtatcc
      721 ggcgtcagat gttagacctg aagactggaa atatgtcagc tatcgtaatg aactacgaag
      781 cgatcgagat ggaagtgaaa ggcaagagca aatgttacga gaggaaccat tttatcgttt
      841 gatgatagag tagagaagat gccagctaca ccaccttgta gcttgaaaac tggttagaat
      901 aactacgatt agatttggca ctgcgtatgc agcctgattc ctgcccgtga cagagcattc
      961 ggctcataaa ctgctgagct actcatctga aatccgaaca gttgtccacc atttcagcgt
     1021 gcatctcctg tttgagtcgt ttgattgctt ttcgggatat taacctgtcg gattttgcat
     1081 tagttaatgc tcccatcagg ccttcatctt ttccagtgcc tattttcctg ttctcctttt
     1141 gcacaatata atcagatcat ctgtatcgtt gacgagctcg ctatcttata ggagcattct
     1201 gaataagcta cctccttgta aggaggcttt tactattagt aactgcaatc taatagtatt
     1261 ataacatctc tatggttgtc ttctatg
//
LOCUS       DAZXUK010000505         1279 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZXUK010000505 DAZXUK010000000
VERSION     DAZXUK010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.509"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="ACH3L8_004412"
     CDS             complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="ACH3L8_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFT4087215.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          358 a          230 c          287 g          404 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgtgagtcg
       61 acaatggatt ccctgaaatg ttaacattcg tacaaatggg caatgtgatt atattttcgg
      121 gtaatgaagt tagtaggttg ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg
      181 ataatctgcg tagttgattt tcacctacct tcaaatattt taagctctga ggaaattctg
      241 gtagttcctg tagtctgttg ccggaaacaa agagttcttg tagtctatga ggcaattcag
      301 gcaagacagt aaggcgatta ccttcaatac gtaatattgt tagcgttgat ggaagctctg
      361 gtaacgtctc cagactatta aaacgtgccg agagtgattg taaatatggt ggtaatgatg
      421 gcagacatga caaatttcga ttgaatgaaa tattaagctt aatcagttga gatggaagtg
      481 tcgggagtat attcaagttg ttaccattta catgaagttc tgtcaaaagc ggagaatttt
      541 caggtaggga gattaactca ttgttggaaa tattaagttc acgtatatgc aaagggagtg
      601 gtggtaaaga acgtagtttc agtaagctta aattgagaac aggctcccgg gtatccagac
      661 atattttcaa taatcgaacc gcctggatgc gattttctgt tgttgttcct tccctagccc
      721 agtcatccca gatgcggtgg taatctctgg tacactgttc ttctaccgat aaacgagata
      781 tacatgataa gccacaaagg gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt
      841 aacccctcac tattcatatc ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa
      901 aaacaatcct agagctttat cggcgttttc attccggctg gaggaatatg ttcctgtctt
      961 tccagtaacc gtcgattgtt atatcagtct acccatgtta atgtgaccag ttctactttc
     1021 atccagtttg gattgatctt tatcccctat attttcaaac aatttttatc atttaactca
     1081 ttactgtctc gatattccca tagcaaacga tctggttggt tatcccctca aaaaagtcag
     1141 ttgcacgccg taaatccctg ccggtattga gcgaggattt acgggttgtt gttaaggtcc
     1201 gtaatcgggt cacagttttt ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt
     1261 gattatgaat ggtgcagtc
//
LOCUS       DAZXUK010000506         1275 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZXUK010000506 DAZXUK010000000
VERSION     DAZXUK010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.510"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            34..435
                     /gene="gpFI"
                     /locus_tag="ACH3L8_004413"
     CDS             34..435
                     /gene="gpFI"
                     /locus_tag="ACH3L8_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFT4087216.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="ACH3L8_004414"
     CDS             447..818
                     /locus_tag="ACH3L8_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFT4087217.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="ACH3L8_004415"
     CDS             805..>1275
                     /locus_tag="ACH3L8_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFT4087218.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          285 c          396 g          291 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAZXUK010000507         1248 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZXUK010000507 DAZXUK010000000
VERSION     DAZXUK010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.511"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(153..419)
                     /gene="ospE2"
                     /locus_tag="ACH3L8_004416"
     CDS             complement(153..419)
                     /gene="ospE2"
                     /locus_tag="ACH3L8_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HFT4087219.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(601..1002)
                     /locus_tag="ACH3L8_004417"
                     /pseudo
     CDS             complement(601..1002)
                     /locus_tag="ACH3L8_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          320 a          261 c          257 g          410 t
ORIGIN      
        1 taagttggca gcattaccgt atcgaggata gatggagtta ctcctctctg aaatagaatt
       61 actgctctac tttttctgtt tccagaaatt attagtaaca aaccctgata tattcttttg
      121 aaaatggagc ttaaacgagt ctgaaattaa cttcagaaat cagaataccg ttggctttgt
      181 ttttcatcaa ttccggcttc atttatcttt gtcaaccatt gcatttctgt agcagtgaat
      241 ttcaacgttt tcgtccggtt ggcagcaggt atttgtaatt cgtatatagc tgctttttta
      301 ttgaaaacag tatatccatc tgtagtaaca gtagaagata ataatacagg atttgatact
      361 tcaaggatga tattttcttg tttttgtgga aggcaaggga atatagtttg tgtaagcata
      421 ttgcttcctt atgttatatt ttagtaaaga atttattttt tacattatag tcggctaaat
      481 gaagtttact gtaaaaaaac gatacttttt tcgtaagcga tgagatgtgg agccagaaaa
      541 atggctgcgt tacgtcattg aactgtcctt gacccacgat attcagacag tcgttgtatc
      601 ttaagttgac attacccgct gacgtcgata ttcccttggt gagagataac ccaacgcact
      661 atgcggatgg ttttcattgt aatgctcgat cgccactgca aggttatgca atgccgttct
      721 tggtttcggc atgaacgcga tgcagtcttc cttcatcgtt ttcatgaacc gttctgctat
      781 tccattactc tgagggctac tgactgccgt cgtgcagggc tccagattca actctctggc
      841 gaactgccgt gtttcatgag ccgtataagc cgaaccgtta tcggccactg gattggccgt
      901 tccggtcctt tatcgccata tcgctcctga catgacatcc tgcaccgttg ctttgtcata
      961 atctcccgtg cctgctgccc agtctatggc ttcacgatcg caatatggaa cgtaacgctg
     1021 tcatcgggag aatcgtcaat gcggcattcg gttaacgctt accaatatca acaccaaccg
     1081 gatttgtgga gcctccagtc tgaaggggct ttatgcgttc cgtataggat attatgatgc
     1141 tggagttttg cgaagctcca ctcctttccg aacatttaca cagatattgc ctaagaaaag
     1201 tgaaaccata atatggttat agtccctttc agtgcaaata cttagctt
//
LOCUS       DAZXUK010000508         1243 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZXUK010000508 DAZXUK010000000
VERSION     DAZXUK010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.512"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            188..367
                     /locus_tag="ACH3L8_004418"
                     /pseudo
     CDS             188..367
                     /locus_tag="ACH3L8_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(384..1074)
                     /locus_tag="ACH3L8_004419"
                     /pseudo
     CDS             complement(384..1074)
                     /locus_tag="ACH3L8_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1153..1243
                     /locus_tag="ACH3L8_004420"
                     /pseudo
     CDS             1153..1243
                     /locus_tag="ACH3L8_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          339 a          269 c          307 g          328 t
ORIGIN      
        1 ggttttctat ggaattcttt ttttattgtg gctgcagtca ataataacac tgaattcaac
       61 attccagtga gcaaggaaat gtaagccgag taagtatatt tgttttacat catattttag
      121 tcaagctcat ttattcctat tatagaatga caaattaaaa ttactgtaaa aaaacgatac
      181 acatttaata cgagggaggc cggcagaaaa acggcttgcg gtcaggcagg agaaatgtgt
      241 gccgcgaatg aagtccttgt atgactggat aaagctccag aggaaaactt tgtcaaacca
      301 tgctgaaatg gtaaaggcgt tcaactatat cctgaatcac tggaatgcac tgaatgagtt
      361 ttgccgggta gtacttccaa tcattagaat atgtgctttt caatgaagga accgatgact
      421 ttttcgtgga tttccacgga acgcgagaag cagattgttt tacgtgccag ccgcttgatg
      481 cgggggcgga gggtcaggtt gttacgttca tacgttgcgt gaagattttg ccggtcaggt
      541 gtttctcctc cggcacttct ctggcgtagc tgccccagtc atcgctggtc accatgccga
      601 tacagaacgg agtcaacagg gctaacagtt cccgacaggt ctcatcattc cgtggcccgc
      661 aagtgtaagc caacacaccg ccagttttag tgttgtacgc ataccagggc cagtgttgtt
      721 gcgccttgct gccaacgaag cgccactgct catcaagttc acagataagg gcaacatcag
      781 cgtgagcgac tggcgcagat gttatccgcc ggggcgagag ctttttaatg tccgtatgac
      841 ggtattgatg ccgattttca gtgtcctggc ggtatcacgg ccccctgcgc cgttatgtgc
      901 catttcaacg atctgctcct taacgccgag ttttctggct tcataagagt aagtgagctg
      961 gaaaacacgt ttacatgagc gacaacggaa acgctcatgc cgggaacaac tacggccatg
     1021 tcgataaact tcatcggaat tacagcgcgg acaatgaaca gtaacggtag tcatggaaaa
     1081 cctcaaatac cagtagatta ccagaaactt ctatcgattg aagacattac cttttgccgc
     1141 aataggtggg gtaacattgg tgaaaacgca ttacgccggg tggcagttgg aaaaaaatta
     1201 tcttttttcg gctctgatcg tggtggagaa aatgcggcaa taa
//
LOCUS       DAZXUK010000509         1237 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZXUK010000509 DAZXUK010000000
VERSION     DAZXUK010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.513"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..572
                     /locus_tag="ACH3L8_004421"
     CDS             <1..572
                     /locus_tag="ACH3L8_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087220.1"
                     /translation="SFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNACHKV
                     AIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGFVSF
                     VNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYMLDE
                     KVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(681..939)
                     /locus_tag="ACH3L8_004422"
                     /pseudo
     CDS             complement(681..939)
                     /locus_tag="ACH3L8_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            923..1237
                     /locus_tag="ACH3L8_004423"
                     /pseudo
     CDS             923..1237
                     /locus_tag="ACH3L8_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          385 a          218 c          291 g          343 t
ORIGIN      
        1 tctcgtttaa gtctttaggc tatatttttc atgatatata taataaaaaa cacactatcg
       61 atgagttcaa cgatgtggta agaaaagcag ttttatcagg taaaattaac gagctgaatg
      121 catgtcataa agtggcaatt tttcttgccg aaaaagacaa tgagataaca aagaaagaca
      181 aagccaagat aatagataca ttaactgaaa actattccat tgaatttcaa caattaatga
      241 atattagtga gcgcacgtta aattcatctt tatatattac tcctggtgaa agtggttttg
      301 tcagttttgt taatcgtgaa ggaaaaatat gtcatactgc atatgtaaaa tcatcagata
      361 atagcatgac atattaccat gcaaatggta gcagcattga caaatatatt acggatatgt
      421 gcggccttat ttgtatgagg catattgagt ctactggcat tattttttat atgcttgatg
      481 aaaaagtttt atctgccatt gctgaattta tgaatgaaaa aggatggcgg gctgcgtttt
      541 gttcagcaaa gaacttatat aaatgtgttt aattttagaa aaactatttg gtaaaatcca
      601 acaagagtcg agaggttcgc gttgcttcag cagggcctca gcctgttcaa ccagactcag
      661 ttgcaggtcg tgacgcagat ttagtggtgg tggttgattg tcagatacca gccgatgatc
      721 ttgtagtgca tatcatcaga ctttgagaag catatggttt tgcgggtaag ccgcttaaga
      781 tgtgtccgca ggttcagatt atgtcgctct atccgctgag tgcatttctt gcttataaca
      841 tgactggttg aggacaataa agttcggtat accggctcag gcatcggtca tgtagaaggc
      901 aatgctaaac tggctcagca aactaagagc agcgcgcatc cacttaccca atgcggttga
      961 gaaaatagcc ttcgatcgct ttcatgtggc gaaacaaccg ggtgaggtgg tggataaaac
     1021 ccgccagaat gaacatccgc atcttcctgt tgaaagtcgt cgtcaggcaa agggaactcg
     1081 cttcctgtgg cagcacagcg ataaatggat gaccgaatcc aggcaggaaa agctgatatg
     1141 gctgcgtgcg cagatgaagc tgacgagcct gtgctgggcg ctgaaagaac tggcaaagga
     1201 tatctggagc agaccatgga gcgaggaaag acgcaac
//
LOCUS       DAZXUK010000510         1225 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZXUK010000510 DAZXUK010000000
VERSION     DAZXUK010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.514"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(170..1057)
                     /gene="rhtA"
                     /locus_tag="ACH3L8_004424"
     CDS             complement(170..1057)
                     /gene="rhtA"
                     /locus_tag="ACH3L8_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFT4087221.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT          370 a          303 c          288 g          264 t
ORIGIN      
        1 taaagattca atgagttaga tatattgata agaacaattc ttatttatct gctcgccagg
       61 aattttcgga ataaatatgc ttttgcataa ttcgatatat aaatacggtg tagaggaaaa
      121 gtagcgagaa attctgcatg gttatgcata accatacaga aatgtaaatt taattaatgt
      181 ctaattcttt tattttgctc tctttgcgta ctgtcagcgt agaccccatt gaagcggcga
      241 tgatagcgcc gagcgccagc agctgtatgg gtgtcagtgt ttctccgagg aaaatcatcc
      301 cggaaacggc agccagcgcc ggttccatgc tcatcagcgt accaaatgtc cgtgttggca
      361 aacgggtgag ggcaatcatt tccagcgaat aaggcagagc ggtcgagaga ataacgacag
      421 ccagacccaa tggaataacc gaccagtgcc agagtgcttc accagcctga agcgctccaa
      481 ttggcacgaa aattaacgct gcaatcaacg aaccaattgc taccgtcgca gggccatgtt
      541 ccgctcctgc gcgttgccca cttaaaatgt aaatagccca acaagccccg gcccccagtg
      601 ccagcgcaca gccggttaaa tcgacatggg aaacgtcttg ccccagcggt agcaggaacc
      661 acagaccaag aaccgccagc acaacccaga cgaaatctac cgggcgacga gaagagaaca
      721 gcgccaccgc cagtggtccg gtgaactcca gcgccaccgc aatacccagc ggtactgtct
      781 gaatagaaag ataaaaaaga taattcatcc cacccagcga aacgccgtaa aacaacagcg
      841 gtaaccgttg ctctttggca aagcgcagtc gccatggctt aaagaacgcg atgaggatca
      901 gcgttcctaa tgccagacgc agcgcagtga cacccggtgc gctcaccaga ggaaaaagtg
      961 acttagctaa cgaggctcca ccctgaatag acgccatggc aacgagcaat atgactattg
     1021 gtaaccagac cggcatttta cgtaatgaac caggcatcct ttctcccaca aatatctaga
     1081 cttaagtaaa gcgtggagtg tactggatat acccaatgct ggttgagcat ttgttgaaaa
     1141 aattttcccc cgttttgact aaaatgcgcc aggattgatg gaatcattag tctggtgatt
     1201 aggaataatc tggatgaatg acagg
//
LOCUS       DAZXUK010000511         1222 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZXUK010000511 DAZXUK010000000
VERSION     DAZXUK010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.515"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(256..>1222)
                     /locus_tag="ACH3L8_004425"
     CDS             complement(256..>1222)
                     /locus_tag="ACH3L8_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFT4087222.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          363 a          273 c          263 g          323 t
ORIGIN      
        1 tgtattagcg actgaaatct gccttttaaa aaggaggagg gtactgagac aaagagtcat
       61 tgcttctccg ataaaatgtc agagaagcat gattacgtgg ttattgtctg ttgatgactt
      121 gttgaccgag ccgtcatcat tctcaatgca ttagttaaat catttgtgat ttttaaggat
      181 tatcctgtct atgattttct acttagaagg atatggcact aactatgcat cttatagttt
      241 actgctggca tctatttaaa atagcaattg agtcaaatag cccatcaaga tagccatacc
      301 taatataacc gtcaggaatg cagctatcat cggcattctg aacattgatt taagtaatat
      361 cacttccgtc aggcttgcgc cggcactgcc gatgattaat gccattaatg ctcccagacc
      421 cattcctttt gtcatcagaa cagatgccag agggataacc gcctcagccc ggatatacag
      481 cggaataccg acaacggcgc ttaatgggat ggcgaaggga ttatctgctc ctgcatgagc
      541 ggcaatccac gctgagggaa taaagccata aataaaagat cctattaata ctcctaacaa
      601 aagatatggc agtacatctt taaactgttg caacgcatct ttggtggcta gttttatggg
      661 attaatactt actacagctt tggtactgca acaggtaact acggggttct caatggcttt
      721 agccgctgga ctacaacaac tcacttttat gggcgtatat gtcgttcccg gcgaagtttt
      781 tgctggagca caacaatttg ctgatgagct tttactggca atgatatgac gttcaaaccc
      841 cagagaatcc aggataatac tggcaagaac ggagacgccg gctgcgataa tcgcgtacaa
      901 caaggtgact ttccaaccaa aggtcatcca cattagtccg acgataatgg gattaagtaa
      961 tggtgaaaca aataaaaaag tgagagtcgg accaaacccg gctttcgctg acaacaatcc
     1021 acgtaacatg ggtattgtcg agcaactaca gaacggggta acggctccca acagagcagc
     1081 caggagatag cctctccctt ttctggcccc catcatctgc tggattttat ggtctggcac
     1141 cttttgtctt atcaggctga caccggcact aatcactata aacagcaacg aaagctcgac
     1201 ggcgagaaac acaaacatct ct
//
LOCUS       DAZXUK010000512         1218 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZXUK010000512 DAZXUK010000000
VERSION     DAZXUK010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.516"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            9..410
                     /locus_tag="ACH3L8_004426"
     CDS             9..410
                     /locus_tag="ACH3L8_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFT4087223.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            398..832
                     /locus_tag="ACH3L8_004427"
     CDS             398..832
                     /locus_tag="ACH3L8_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFT4087224.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
BASE COUNT          302 a          286 c          350 g          280 t
ORIGIN      
        1 atatgactat gtacgcaaaa tcctttatcg ctcttgatgg caacggacgt ctgacgggcg
       61 cccgtactgc acaggccgca ccttatgcta actacacctg ccacttgtgt ggcagtgcac
      121 tcagatacca tccgcaatac gaaactgaac ttccctggtt tgaacacact gacgacaggc
      181 tgacagagca cggtcaacag tgcccttatg tcaggccgga gcgcagagaa atacagttga
      241 ttaaacgtct gcagcaattc gtaccggatg ccttacccgt ggtgcgtaaa gccagctggc
      301 actgcagaca atgtcaccac gattattatg gggagcagta ctgcacacac tgccagaccg
      361 gaggattcag tattccccgg acaactcagg aggaaatatg cgaattctga actgttatat
      421 ggcgaatgac agcaaaggcc attttgttac ggcgaaagaa gctgcgaagc acaaccgaca
      481 ggacgttttg tgctgtgtgt cctgtggatg cccgttaaca cttcagcggg gcaatgacgg
      541 acaaccaccg tggtttgaac atgaccagat gactgtcgct gaaaaaatcc tgctgcgatg
      601 tacctggctt gacccggcag agaaagaggc ccgtcgtttg catctgcagg gcatgacggt
      661 tccggattat acggtgaagg tgagaaagtg gttttgtgtg atgtgtgacg aagattatga
      721 gggggaaaag tgctgcccac gctgcggtac cggggtatac agcagggcgt gggggcggca
      781 ggaggtgccg tcggaagatg ccagggctga taatccgtta cagaggctgt aatggttgcc
      841 tccggagcag tttgcggtga tgcctttccc gggaatggat tgccagtgcg gggactgtgg
      901 agcttaattc cggtgttggt ggcctttccg ttgattttat gccaacagcc ccctgtgact
      961 gacaggctgc ctcgtcattc cattcgtatc tccagtaaca gggggttgta ttgtatttta
     1021 ttgtgccggt tcaggtgtgc gtttcccggc gcgtctgcac cggcttaacc aaattcaaca
     1081 gggataaaat agtggtaagc gtacagcctg aaccgtctgg tcagaatctg acgaattaga
     1141 caaagtggtg tccaccaaat aagtagtggg aaccaaagtg tcagatatgc agaaaaatgt
     1201 gactcccggc aggcgaaa
//
LOCUS       DAZXUK010000513         1211 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZXUK010000513 DAZXUK010000000
VERSION     DAZXUK010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.518"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            7..1188
                     /locus_tag="ACH3L8_004428"
     CDS             7..1188
                     /locus_tag="ACH3L8_004428"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFT4087225.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
BASE COUNT          261 a          277 c          371 g          302 t
ORIGIN      
        1 gttgtgatga aagacgttgt gattgtcggg gcgttacgga cacctatcgg ctgctttcgt
       61 ggtgcgttag cgggtcattc cgccgtggaa cttggtagtc tggtcgtgaa agcgttaata
      121 gaacgtaccg gcgttcctgc atatgcggtg gatgaagtaa ttcttggtca ggtgttgact
      181 gcaggggcag ggcagaatcc ggcaaggcaa tcggctatta aaggtggtct tcctaacagc
      241 gtttctgcaa tcacgattaa tgacgtttgc ggttccggtc ttaaagcact gcatctggct
      301 actcaggcga tacagtgtgg cgaggctgat attgtcatcg caggtggcca ggaaaacatg
      361 agccgcgcac cacatgttct gactgatagc cgcaccggtg aacagcttgg caatagccag
      421 ttggttgaca gtctggtgca tgatgggttg tgggatgcct tcaatgatta tcatattggt
      481 gtcaccgccg aaaatctggc tcgcgaatat ggcatcagcc gtcagttgca ggatgcttac
      541 gcacttagct cgcaacaaaa agcgcgagcg gcgattgacg ccggacgatt taaagatgag
      601 atcgtcccgg taatgatcca aagtaacggt cagacgttgg ttgttgatac cgatgaacag
      661 ccacgcactg acgccagtgc agaaggttta gcccgtttaa atccttcatt tgatagtctc
      721 ggttctgtga ccgcggggaa tgcatcatcc ataaacgatg gcgcagctgc ggtaatgatg
      781 atgagcgaag ccaaagcacg agcgttgaat ttacccgtgc tggcccgcat ccgcgcattt
      841 gccagcgttg gtgtagatcc ggcattgatg ggaattgcgc cggtgtatgc gacccgccgt
      901 tgcctggagc gtgtaggctg gcagttggct gatgtcgatc ttatcgaggc taatgaagcg
      961 tttgctgcac aggcgctttc ggttggcaag atgcttgagt gggatgagcg tcgggtcaat
     1021 gtcaatggtg gcgcgatcgc actcggtcac ccgataggcg cttccggttg ccgaatcctg
     1081 gtttctctgg ttcatgaaat ggtgaaacgt aatgcccgca aagggctggc aacgctttgt
     1141 atcggcgggg gccagggcgt ggcattgacc attgaacgtg acgagtagct ctgcatttcc
     1201 tccttaccga t
//
LOCUS       DAZXUK010000514         1210 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZXUK010000514 DAZXUK010000000
VERSION     DAZXUK010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.519"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1093)
                     /locus_tag="ACH3L8_004429"
     CDS             complement(<1..1093)
                     /locus_tag="ACH3L8_004429"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFT4087226.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYE"
BASE COUNT          298 a          312 c          308 g          292 t
ORIGIN      
        1 actcatacgg ccccagtttg gcgatctcat ccgccagata ggcagcgacc tggtaggaag
       61 cattctgtac tttggtatag ccttcacgac caaggcgcag gaattcatag tactgtgcaa
      121 tcacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatctga ccaccgaggt
      181 agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag ataacccagc
      241 cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat ttcacacgcg
      301 gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccagaaag ccaccgctgg
      361 cggcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta tccagtgcat
      421 cgtgcagcgg ttgcgggaac tcatagttac cggtgtaggt cacgccgaaa gtcggcacca
      481 cgccgatggt attttcgtcg caggcttcaa tcatgcgttt cgggtccata aacaactgac
      541 cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat ttatgccagc
      601 agatttgcac cggaccgcac accaggtttg gtttatccgt tggtttgcct gcagcttcca
      661 tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc tcggaagaac
      721 caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc cacagatcgg
      781 caaccatgtt tacgcaacgc aggtcgatgg ctgcggattg cggatattct tctttgtcga
      841 tccagttttt gttaatggat aaatccatca acttgtggac gttttcgtcg tcccaggtct
      901 ggcagaaagt ggccaggttc tgacgagcgt tgccatcaag atataattca tcattgataa
      961 tctgaaatgc gacatcgtcg cgcatttcgt gcagcggaaa acgttttgat tctgcgatag
     1021 tggaaataga cttcgcacca aaacgtgaat cgagtagttc cgaccttaaa tccgttactt
     1081 gcttcttatc cattttaaac tccttaaaat gattggatcg cattaaaaaa gtaggattta
     1141 tcgataaagt aagcaagtgc aaaggactcg tgtttaaata acaaaatcct aatgttattt
     1201 atcgtgagat
//
LOCUS       DAZXUK010000515         1205 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZXUK010000515 DAZXUK010000000
VERSION     DAZXUK010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.520"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..446)
                     /gene="ospG"
                     /locus_tag="ACH3L8_004430"
     CDS             complement(<1..446)
                     /gene="ospG"
                     /locus_tag="ACH3L8_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFT4087227.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDK"
     gene            complement(529..698)
                     /gene="yacB"
                     /locus_tag="ACH3L8_004431"
                     /pseudo
     CDS             complement(529..698)
                     /gene="yacB"
                     /locus_tag="ACH3L8_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            complement(698..967)
                     /gene="yacA"
                     /locus_tag="ACH3L8_004432"
                     /pseudo
     CDS             complement(698..967)
                     /gene="yacA"
                     /locus_tag="ACH3L8_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
BASE COUNT          319 a          302 c          246 g          338 t
ORIGIN      
        1 tctttatcat acagcatatt acctgtatta agatcgtaat ggattatact caactcattc
       61 aatttacata tcaactgtag ataaaggctc tctatattat cagggatatc agctgtatca
      121 atgtcactaa ggggagtccc aggcacgcgc agcattcgga ggtacacatc tccgccatac
      181 tgtataacaa cggatgcttc atcgccataa aaagcattaa aaagctcaga ttcttgccaa
      241 gccatttcca gaatctcatt gtactggttg ccaataagat catacttttt atacaaagca
      301 gatgaatcat tcacatcttc aaagatttct gctgtcgacc cctgacctat taacttaccg
      361 agaatgggct ccgttactat gccagcatga gaattattat tctcaaatgg aaaaggtgtt
      421 tgaataatgg tagatgttat tttcatgtca tacctttgtt acgatattta aaaatcacaa
      481 gagattttca cacaaaataa cgttacacgc taccttgtgt ggtccagatg ttccttccac
      541 acgaccgttg cggcctttat acggctgccc tgccaggctg ctggctgaat aaccaatccg
      601 ttcgtccggt tcaatggcgg ccatcaggtt ctgctccgca atgtactacg aatgcgcttc
      661 ctgtcctgga tggccagcat ggtccagaac atctccatca tttatcagca atcttcctgc
      721 tcatttcatc ccgccatgca gcagcagagg attcgaccat gtcatgggga agcacatcac
      781 cgcattcgag ttgctgacgg cctgccgcaa cctagtcacg gaaccaagaa ttgtgctgcc
      841 gttcaacggt ctggcgcata aaatcccgga ttaactgaga gccattacgg tccatgcttt
      901 ttgcagcggc cataaaagca tccttcagtt ctgcgtcgat tcttacactc atattaacct
      961 gtgccatttg ccacctcgtc attaccacgt atatacacag tatataactg ttcagatatc
     1021 gtcaccagga tatgccgcgc cagcggcatg taaggcggca ctccgttgtt gcatatggca
     1081 gaggagtaaa accgccgaag cccggcgtaa gccggtacgt attgacaggt ttcacctgtc
     1141 ctatcactgg cgctgacagt tttatgcgat gtgattatga atggtgcagt tgtgagccgt
     1201 tttca
//
LOCUS       DAZXUK010000516         1201 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZXUK010000516 DAZXUK010000000
VERSION     DAZXUK010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.521"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            353..>1201
                     /gene="ipaH1.4"
                     /locus_tag="ACH3L8_004433"
     CDS             353..>1201
                     /gene="ipaH1.4"
                     /locus_tag="ACH3L8_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH1.4"
                     /protein_id="HFT4087228.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPQIFFSMGNSA
                     TISAPEH"
BASE COUNT          413 a          246 c          200 g          342 t
ORIGIN      
        1 aaatatatca gggtttgtta ctaataactt ctgaaaacag aaaaaaaata gagcagtaat
       61 tctatttcag agaggagtaa ctccatctat cctcgatacg tactgaacgt tatcgtgata
      121 tattcatcat gctttcttca ccattattcg agtataggga gagacgcaat gaccttaaat
      181 acctttttat ggctgaatta ccattctcag gaataacagc agactccttc ccatagacgg
      241 cttaggggga aagcaatacc cggagaaagg gtacaaagca aactgtttta aaattaataa
      301 gccatattct atttaactag aattcttatc gttgataaga attctagtta taatgattaa
      361 atcaaccaat atacaggcaa tcggttctgg cattatgcat caaataaaca atgtatactc
      421 gttaactcca ttatctttac ctatggaact gactccatct tgtaatgaat tttatttaaa
      481 aacctggagc gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa
      541 taggctgaaa atatgtttac aaaatcaaga ggcagaatta aatttatctg agttagattt
      601 aaaaacatta ccagatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt
      661 aacacatctc cctgatttac caccaatgct taaggtaata catgctcaat ttaatcaact
      721 ggaaagctta cctgccttac ccgagacgtt agaagagctt aatgcgggtg ataacaagat
      781 aaaagaatta ccatttcttc ctgaaaatct aactcattta cgggttcata ataaccgatt
      841 gcatattctg ccactattgc caccggaact aaaattactg gtagtttctg gaaacagatt
      901 agacagcatt cccccctttc cagataagct tgaagggctg gctctggcta ataattttat
      961 agaacaacta ccggaattac cttttagtat gaacagggct gtgctaatga ataataatct
     1021 gacaacactt ccggaaagtg tcctgagatt agctcagaat gccttcgtaa atgttgcagg
     1081 taatccattg tctggccata ccatgcgtac actacaacaa ataaccaccg gaccagatta
     1141 ttctggtcct caaatatttt tctctatggg aaattctgcc acaatttccg ctccagaaca
     1201 c
//
LOCUS       DAZXUK010000517         1200 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZXUK010000517 DAZXUK010000000
VERSION     DAZXUK010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.522"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            72..1115
                     /locus_tag="ACH3L8_004434"
     CDS             72..1115
                     /locus_tag="ACH3L8_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFT4087229.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT          339 a          267 c          272 g          322 t
ORIGIN      
        1 ggacttgata cacagaatct caacttttat gctctcagac tatatcacca catgttttac
       61 aggctcagaa aatgaacgat agattatgct ttgaagtgca cgataacaag ggttatttcg
      121 tatttccgga tacctggttt ggcccattgc tgggggagtt tgaagaagtg cttgatgcat
      181 acgacgctga tgaaatcagc gagacgagtt acatcaacaa actccgacgc cttgctcaac
      241 gggagccgga ctttattgat atacacgctc atctggcata tgcatttctg gaacagaatg
      301 ccccgcgtaa ggcactgaac gccgctctta aaggactggc ggcaggtaat cgtatcatcc
      361 cggaaagttt ctgtggtgaa ataatctgga tgcatcccga aaaccggcct tatctgcgcg
      421 cgctttatgc agcaattctc gcaaatgtgc acttacagcg ccatcaggat gcggttatgc
      481 tcactgataa aatactggca tacaatcctg aagataatca gggggctcgc tggttgttgg
      541 gttcagagct gttacgcacc ggcgatcatg agcgagcctt cagtgtactg aaagagcatg
      601 ctgatgaatt ttcaccttac tggtatgaac tcggtctcct gcattttctg aacggggaac
      661 atgttaaggc cgccacagct tttcgccatg gctttgccac aaacacttac atcgcagaaa
      721 tgctgtgcgg aaatctgcat ccatttccac ttgctgtgtg gcatgacttc tccggctctc
      781 tggatacggc agaagattac tatgcaacat actcaccatt atggggacaa tatccggaag
      841 cattgctgtt tgtaaactgg ctctataacc attcctccgt gctgcatgaa cgatcagaaa
      901 tcattaaatg cgccgaaatg ctgattcagg aagatgattt cgaaatatgc gaaagtattt
      961 taagacagca ggagcacctg tggaaaagaa ttgacaagac actttctgag gaaattgtac
     1021 agaaatgcag aaatatgaac ggtgaatata tctggccctg gatattgccc ttttccgcag
     1081 cagggatgaa acattcaagc atccagtatc agtagatatt gcattagtgt aactttaaaa
     1141 caagtaataa tttattatcc aataataaca ctgtgttcct tccccaggtg attaccctgg
//
LOCUS       DAZXUK010000518         1189 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZXUK010000518 DAZXUK010000000
VERSION     DAZXUK010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.523"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            118..720
                     /gene="ais"
                     /locus_tag="ACH3L8_004435"
     CDS             118..720
                     /gene="ais"
                     /locus_tag="ACH3L8_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFT4087230.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQNKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACH3L8_004436"
     CDS             complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACH3L8_004436"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFT4087231.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          317 a          272 c          256 g          344 t
ORIGIN      
        1 ggcattttgc cgacatcgta ggatttttaa tattacacca actgctaatt atcgccagaa
       61 atgtcgctca atttcacggt aattgtctgg ttgcgcttgt ctataggtgg agtttacgtg
      121 ttagcttttt gccgctcatc attgaagtca aaaaaatatt tcatcatttt actggcgctc
      181 gctgcaattg ccggactggg tactcatgcc gcctggagta gcaatggttt gccacgtatc
      241 gacaataaaa cattggccag actggcacag cagcacccgg ttgtcgtttt gtttcgccat
      301 gctgaacgtt gcgatcgttc aaccaatcaa tgcttgtcag ataaaacagg tattacggtt
      361 aaaggtaccc aggatgcccg tgaactgggc aacgctttta gcgctgacat ctctgatttc
      421 gatctttatt ccagtaatac cgtccggacc attcagtcgg cgacctggtt ttccgcgggt
      481 aaaaagctga cggtagataa acgacttctt cagtgcggta atgagattta tagtgcaatt
      541 aaggacttac aaaacaaagc gcctgataaa aatatcgtta ttttcaccca taatcattgc
      601 ctgacatata ttgctaaaaa taagcgtgat gcgatattta aacctgatta tctggatggt
      661 ttagtcatgc atgtggaaaa aggcaaagtt tatctggatg gggaattcgt taaccactaa
      721 attttcaatc tgacagccag tgatggctgt catcattgtt aaagaagacc tttcaaacgt
      781 aacgtttttc gggtggcaac atttaaatca taatgcacca gatcttcagg ttttacccac
      841 gcgtagtcct gaaactcttc gtttattttc acttctcggt tggcagaaac gcagtcaaaa
      901 atcaggtaaa tcatataaat ctcttccttg cgaccatctg catatgtctt ggtgcgaata
      961 tcatcgctga aggtccacgg cgtgatttct gtcaaaagca gctgttctcc cagttcttcg
     1021 cgaatttcgc ggcgtagtgc ctcttcaatt cgttcgccag actccacgcc gccacccgaa
     1081 agcgcccatt gaccggggaa aacgccgcga tcgtcggcca ttttgcacag caaataagca
     1141 ccatcatttt gaatcaaagg gcatacaata gtccgttgtc gcacagggg
//
LOCUS       DAZXUK010000519         1187 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZXUK010000519 DAZXUK010000000
VERSION     DAZXUK010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.524"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..205
                     /locus_tag="ACH3L8_004437"
     CDS             <1..205
                     /locus_tag="ACH3L8_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087232.1"
                     /translation="TKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNL
                     GSNLTSSIKAKKNAITILNTLNL"
     gene            318..1156
                     /gene="aadA1"
                     /locus_tag="ACH3L8_004438"
                     /pseudo
     CDS             318..1156
                     /gene="aadA1"
                     /locus_tag="ACH3L8_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          327 a          285 c          301 g          274 t
ORIGIN      
        1 tacaaaactg tatgggaaaa ctggtgcagg attcacagca aatagaacct tacaaaacgg
       61 atggtttgaa gggtttatta taagcaaatc aggacataaa tatgtttttg tgtccgcact
      121 tacaggaaac ttggggtcga atttaacatc aagcataaaa gccaagaaaa atgcgatcac
      181 cattctaaac acactaaatt tataaaaaat ctaatggcaa aatcgcccaa cccttcaatc
      241 aagtcgggac ggccaaaagc aagcttttgg ctcccctcgc tggcgctcgg cgccccttat
      301 ttcaaacgtt aaacatcatg agggaagtgg tgatcgccga agtatcgact caactatcag
      361 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
      421 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
      481 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
      541 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
      601 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
      661 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
      721 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
      781 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
      841 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
      901 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
      961 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     1021 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac tacgtgaaag
     1081 gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct
     1141 tcgcggcgcg gcttaactca agcgttagat gcactaagca cataatt
//
LOCUS       DAZXUK010000520         1182 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZXUK010000520 DAZXUK010000000
VERSION     DAZXUK010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.525"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            126..1124
                     /locus_tag="ACH3L8_004439"
                     /pseudo
     CDS             126..1124
                     /locus_tag="ACH3L8_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
BASE COUNT          267 a          259 c          267 g          389 t
ORIGIN      
        1 tgtctttaat gatatctcgt cgttatatta acaggctgaa attatcatta atgcacattt
       61 caatattagc agggatacct gaggagttta aagtgaacat atatatcggg tagcttttca
      121 aattaatccc tttgattata ggcttaattt gcatcgcgtt aggtggcttt gtgctggaaa
      181 gttcagggca acgtcacggg tcatgtgctg atttctctgg cggccatatg cctggcatta
      241 ttcactaccg catttattat catttcgcaa ctcacgcgcg gcgttaatac gttttacaat
      301 acattgttcc ccattattgg ctatgcgggg tcaattatca ccatgatatg gggttgggca
      361 ctgttagcag gtaatgatgt gatggcagac gagtttgttg ccggccatgt tatttttggc
      421 gttggtatga ttgccgcatg tgtatcgacg gtggcagcgt cgtccggtca ctttctgctc
      481 attcccaaaa atgcagcggg gagcaagagc gacggaacac cggtacaggc ttattcttca
      541 ttaatcggta attgcctcat tgccgttccc gttttactca ccctgctcgg tttcatttgg
      601 tctattacgc tgttacatag tgccgacata actccgcatt atgtcgcctt gggttaaccg
      661 caatctgtgc ctgtctaatt ggcctagtgg ccacaatcgt ccatcaaaca cgtaatacgt
      721 tctcatccaa agagcactgg ctgtggtgtt attgggttat ttttctcggc tcagtcacgg
      781 tgctgcaggg gatatacgtc ttagtcagtt ccgatgcaag cgcccgactg gctcccggca
      841 ttattctcat ttgcctcgga atgatctgtt acagcatatt ctcaaaagtc tggctactgg
      901 cactggtctg gagacgtacc tgttcgttag ccaacagaat accgatgata ccggtattta
      961 cctgtctgtt ttgcttgttt ttgtcatcat tcctcgcgga aatcgcacaa actgagctgg
     1021 actactttat tccatctcgg gttctggttg gattaggcgc agtatgtttt acattgttct
     1081 ctattgtttc aatattagag gccggttctg cgaaaaaaaa ttaaaattta cataatccca
     1141 gcgttattca tatcttaacg ctggtttttt acgaacttag tg
//
LOCUS       DAZXUK010000521         1179 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZXUK010000521 DAZXUK010000000
VERSION     DAZXUK010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.526"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            34..1026
                     /gene="yiaK"
                     /locus_tag="ACH3L8_004440"
     CDS             34..1026
                     /gene="yiaK"
                     /locus_tag="ACH3L8_004440"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFT4087233.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILP
                     MGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRD
                     AKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1038..>1179
                     /locus_tag="ACH3L8_004441"
     CDS             1038..>1179
                     /locus_tag="ACH3L8_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFT4087234.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT          288 a          320 c          308 g          263 t
ORIGIN      
        1 caaaaaagcg atcaaaaaac aaggaagcct aggatgaaag tgacatttga gcagttaaaa
       61 gcagccttta atcgggtctt aatttcacgc ggcgttgaca gcgaaacggc tgacgcctgt
      121 gcagagatgt tcgcccgcac caccgaatcc ggcgtttatt ctcacggcgt taatcgtttc
      181 cctcgtttca ttcaacaact ggaaaacggc gatatcattc ctgatgccca acccaaacgt
      241 ataaccagcc tcggcgcaat tgaacagtgg gatgcccagc gttcgatcgg caacctgacg
      301 gcgaaaaaga tgatggatcg cgccattgaa ctggctgccg atcacggtat tggtctggtg
      361 gcacttcgta atgccaacca ctggatgcgc ggcggcagct acggctggca ggcggcggaa
      421 aaaggctata ttggcatttg ctggaccaac tccatcgccg taatgccgcc gtggggcgca
      481 aaagagtgtc gcatcggcac taacccgctg atcgtcgcca ttccttccac gccgatcacc
      541 atggtcgata tgtcgatgtt ctcttacggc atgttagaag ttaaccgcct ggcaggccgt
      601 cagctcccgg tcgatggtgg ctttgatgat gagggcaatt tgaccaaaga acctggcgtt
      661 atcgagaaga atcgccgcat tttgccgatg ggctactgga aaggttctgg catgtcgatt
      721 gtgctggata tgatcgctac tctcctttcc gacggcgcat cggttgccga agtcacccag
      781 gacaacagcg acgaatacgg catttcacaa atttttattg ccattgaagt ggacaagctt
      841 atcgacggtc ccacccgcga tgccaagctg caacgtatca tggattacgt tactaccgct
      901 gaacgcgctg acgaaaacca ggccatccgc ttacccggcc acgaatttac taccctgctg
      961 gccgaaaacc gccgtaacgg catcactgtt gatgacagcg tgtgggccaa aatccaggcg
     1021 ttatgaggag ataagtcatg atttttggac atatcgcgca gcccaatccg tgccgtttgc
     1081 ccgccgccat tgaaaaggcg ctcgattttc tgcgcgctac caacttcaac gtcctggagc
     1141 cgggcgttgt cgaaatcgac ggcaagaata tttatgcgc
//
LOCUS       DAZXUK010000522         1166 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZXUK010000522 DAZXUK010000000
VERSION     DAZXUK010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.527"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..102
                     /locus_tag="ACH3L8_004442"
     CDS             <1..102
                     /locus_tag="ACH3L8_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFT4087235.1"
                     /translation="CELVLPAGTLRLKGKLTPALLQILIREIKGSSH"
     gene            102..449
                     /gene="tnpB"
                     /locus_tag="ACH3L8_004443"
     CDS             102..449
                     /gene="tnpB"
                     /locus_tag="ACH3L8_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFT4087236.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            469..>1166
                     /locus_tag="ACH3L8_004444"
     CDS             469..>1166
                     /locus_tag="ACH3L8_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFT4087237.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPVLPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVAGKYADYLPLYRQSEIYRRQ
                     GVELSRATLGRWTGA"
BASE COUNT          269 a          323 c          331 g          243 t
ORIGIN      
        1 tgtgagctgg ttctgccggc cggaactctc aggcttaaag gtaaactgac gccggcgtta
       61 ttacagatac ttatccgcga aataaaaggg agcagccact gatgatatct ttccctgcag
      121 gttcgcgtat ctggctggtt gcaggtatca ccgatatgcg aaatggcttt aacggcctgg
      181 tctcaaaagt tcagaacgtc ctgaaggatg acccgttctc cgggcatctg ttcatcttcc
      241 gcggacgccg gggtgaccag ataaaagtgt tgtgggctga cagtgacgga ctgtgcctct
      301 ttaccagacg cctggagcgg ggccgcttcg tctggccggt cacccgtgac ggcaaggtgc
      361 accttactcc ggcccagtta tccatgcttc ttgagggcat cgactggaaa cacccgaaac
      421 gaacggaacg cgctggcatc cgcatataac ccgttgtaaa gtgaggatat ggacacctca
      481 cttgctcatg agaatgcccg cctgcgggca ctgttgcaga cgcaacagga caccatccgc
      541 cagatggcga aatacaaccg cctgctctca cagcgggtgg cggcttatgc ttccgaaatc
      601 aaccggctga aggcgctggt tgcgaaactg cagcgcatgc agttcggtaa aagctcagaa
      661 aaacttcgcg caaaaaccga acggcagata ctggaagcac aggagcgaat cagcgcactt
      721 caggaagaaa tggcggaaac gctgggtgag caatatgacc cggtactgcc atctcccctg
      781 cgccagtctt cagcccgtaa accgttacct gcctcacttc cccgtgaaac ccgggtcatc
      841 cgtccggaag aggaatgctg cccggcctgt ggtggcgaac tcagttctct gggatgtgat
      901 gtgtcagagc agctggagct tatcagcagc gcctttaagg ttatcgaaac acaacgtccg
      961 aaactggcct gttgccggtg tgaccatatc gtgcaggcac cggtgccttc aaaacccatt
     1021 gcacgcagtt atgccggagc ggggcttctg gcccatgttg ttgctggaaa atatgcagac
     1081 tatctgccgt tataccgcca gtcagaaata taccgtcgtc agggcgtgga gctgagccgt
     1141 gccacactgg ggcgctggac aggtgc
//
LOCUS       DAZXUK010000523         1160 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZXUK010000523 DAZXUK010000000
VERSION     DAZXUK010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.528"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..253
                     /locus_tag="ACH3L8_004445"
     CDS             <1..253
                     /locus_tag="ACH3L8_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein mxiK"
                     /protein_id="HFT4087238.1"
                     /translation="EMVISYYGGKISKQGEAAIRSGFWHLIAWKENISVGIYERINLL
                     FNPIALEGNYTPVERNLSRLNEGMQYAKRHFTGIQTSCL"
     gene            195..890
                     /gene="mxiN"
                     /locus_tag="ACH3L8_004446"
     CDS             195..890
                     /gene="mxiN"
                     /locus_tag="ACH3L8_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HFT4087239.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            883..>1160
                     /gene="mxiM"
                     /locus_tag="ACH3L8_004447"
     CDS             883..>1160
                     /gene="mxiM"
                     /locus_tag="ACH3L8_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HFT4087240.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYV"
BASE COUNT          440 a          139 c          231 g          350 t
ORIGIN      
        1 tgagatggtt attagttatt atgggggcaa aatcagtaag caaggggagg ccgctattcg
       61 aagtggtttt tggcatctta ttgcgtggaa ggaaaatata tctgttggta tatatgaaag
      121 aataaactta ttatttaatc caattgcttt ggagggaaat tatacgcctg tagaaagaaa
      181 tttgtcccga ttaaatgaag gtatgcaata tgcaaaaagg cactttaccg gtatccagac
      241 atcatgccta tgatggtgtt gttataaaga gaattgaaaa ggaattatgt aaaaccataa
      301 aagatagaga cactgagagt aagaagaaag caatttgtgt tataaaggag gctacgaaaa
      361 aagctgaatc actgcgtata gatgctgtat gtgatggtta tcaaattggt atacagactg
      421 cttttgagca tattattgac tatatttgtg aatggaagtt aaagcaaaat gaaaatagaa
      481 gaaatattga ggactatata actagtttgc tttcagaaaa tttacatgat gagaggatca
      541 ttagcacttt attggagcag tggcttagtt cgttgcgtaa tacagtaaca gaattaaaag
      601 tggttttgcc caagtgtaat ttagcactga ggaaaaagtt agaacttgat ctccataaat
      661 atcgctcaga tgtcaagatt attttaaaat attctgaagg gaataattat atattctgta
      721 gtggtaatca agtagtggag tttagtcctc aggatgtaat atcaggtgtt aaaatagaac
      781 ttgcggaaaa attaacaaaa aatgataaaa aatatttcaa agagttagca cataagaaac
      841 tgagacaaat tgcagaagac ttgttaaaag agaatcctgt taatgattaa tcaaataaat
      901 gcaagcaatg ctctgcagca aagattaaat agtgaagaag ttgttaattt aaatgagaga
      961 ttatcatctt cgcaatcatt tgatgaggat ataatctatg aaataatgca atatttctca
     1021 cagagtgaat taaattccat agataatgat gaactgcaca ataagattga acaattattc
     1081 aattcacgtt ttccttatct tacagctgca caaaaaagtt cacttcttaa taaactcata
     1141 gatgcaaatc aatatgtcga
//
LOCUS       DAZXUK010000524         1159 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZXUK010000524 DAZXUK010000000
VERSION     DAZXUK010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.529"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            115..>1159
                     /gene="cadB"
                     /locus_tag="ACH3L8_004448"
     CDS             115..>1159
                     /gene="cadB"
                     /locus_tag="ACH3L8_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFT4087241.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          246 a          255 c          298 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctg
//
LOCUS       DAZXUK010000525         1156 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZXUK010000525 DAZXUK010000000
VERSION     DAZXUK010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.530"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..385
                     /locus_tag="ACH3L8_004449"
     CDS             <1..385
                     /locus_tag="ACH3L8_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087242.1"
                     /translation="VLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFI
                     AVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLAL
                     NVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(386..>1156)
                     /locus_tag="ACH3L8_004450"
     CDS             complement(386..>1156)
                     /locus_tag="ACH3L8_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFT4087243.1"
                     /translation="IRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLT
                     PTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSLIDIVQQGFDAGVRLSG
                     IVEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQF
                     AKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITER
                     PGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          295 a          262 c          279 g          320 t
ORIGIN      
        1 ggtgctggtg gcaattctga ttatgctgcc gactctcggc atcggtgata tgaacaatct
       61 ctataaatgg ctgttgaatc ttaactcggt agtgatgccg ctgcgttatt tatgggtatt
      121 tgttgcattt attgcagtcg ttcgcctggc gcagaaatat aaaccagaat atgtctttat
      181 tcgtaataag ccgctggcaa tgaccgtcgg gatttggtgt tttgccttta ccgcctgtgc
      241 ctgtttgacg gggatcttcc cgaaaatgga agccttcact gcagagtgga ccttccagtt
      301 ggcgctgaat gttgcaacgc cgtttgtgct ggtaggatta ggactgatat tcccgctgct
      361 ggcgcgtaaa gcgaatagca aataattatt gtggtcggtg acgctgactc tggcagatct
      421 gacccgtttt tacataatcg agaaatgcgc gcaagccgca ggacatatac tggcggtttg
      481 ggtaatagac ttggaagcct ggacgctctg tgatccagtc ttccagtaca cattctaaac
      541 gaccagtatc aaggtactcc ttgatctgtt catagagcag atacccgata ccagcaccca
      601 tcaaaacggc ctccagttca gcatcaacat catccagaat gatatttcct gcgacagcaa
      661 tttccagctc ctttgcaaat tgccagtgga aaggtttgcc gctgggataa cgaaaaacaa
      721 cacattgatg attaagcaga tcatgaggat ggcggggttt gccatatctt gcaaagtact
      781 ctggcgttgc tgctacacat aatttcactg gtgggccgat cgccacgctg atcatatctt
      841 tttcgacgat gccactaagt cgaacgccag cgtcaaagcc ctgttggacg atatcgataa
      901 gagaatcgtc agttgtgagc tctactttaa tatcaggata ttcacgtgtg aaaccaacca
      961 gtaaaggcat taaaaatata cgtgcagcta cccgggcggc attgattttg agcgttcctg
     1021 tcggtgtcag acgaaaatca ttcatctcat ctaacatgat ctgaatttcg tcgaaagctg
     1081 ggcgcaatcg ttcatagaga ttagaacccg cttccgttaa cgatacgctt cgggttgtcc
     1141 gattgaataa gcgaat
//
LOCUS       DAZXUK010000526         1140 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZXUK010000526 DAZXUK010000000
VERSION     DAZXUK010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.531"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..730
                     /locus_tag="ACH3L8_004451"
     CDS             <1..730
                     /locus_tag="ACH3L8_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFT4087244.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(732..958)
                     /locus_tag="ACH3L8_004452"
                     /pseudo
     CDS             complement(732..958)
                     /locus_tag="ACH3L8_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          298 a          283 c          247 g          312 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
//
LOCUS       DAZXUK010000527         1127 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZXUK010000527 DAZXUK010000000
VERSION     DAZXUK010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.534"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(127..249)
                     /locus_tag="ACH3L8_004453"
     CDS             complement(127..249)
                     /locus_tag="ACH3L8_004453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087245.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(322..1062)
                     /gene="phoN2"
                     /locus_tag="ACH3L8_004454"
     CDS             complement(322..1062)
                     /gene="phoN2"
                     /locus_tag="ACH3L8_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFT4087246.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
BASE COUNT          301 a          241 c          244 g          341 t
ORIGIN      
        1 tgaagttgtc atttctgtta tattgatcga cataggggat ggtgcagacc gttcccctgc
       61 aacaccacat tgcatgtggt gaacggaacc gccccgagtt tcctagagaa tgttttatct
      121 gtgaactcag gttgccagat catcgtttcc gatggcagca taataagctt tttctgcttc
      181 tgccggaggg atatgtccca gcctttccag taatccctta aattcagggg taaaacgtgg
      241 tgtgcccata gactcctcct atgctcatat agggtagatt tgtctatggg ccctccatag
      301 cctgatacag gctgtccagc tttatggggt cagttcattg gtaggagtat ttaattcttc
      361 aaactctttt ttggcttcgc taaggctttt ggtaaattca ggtgtattat gaagtactgc
      421 aacaaccgat gctcccatta aacgcccagc ctctacatcg ctttgccaat gcgcaccgca
      481 gatgacccga ctttctccaa actcatatcc acgtcgaagt atttccgctt tacgttgagg
      541 attaatctcc gcaagtatca gtgctactgc ccaaccaaag gatgcatgac cagagggata
      601 agagccagtg atagccattt tctcatcttt atcaggtgta caggttgcgt ctttataaat
      661 aacgaatgga cgaactcttt tataatattc tttggcgtta cgcacggcgt aatcatggct
      721 atcctgtagt acttgtgtta acaactgata taagattggg gtattttccc ttgaaatatc
      781 catgccataa gcatctgaaa atgcaagacc aaaattctcg tagtatgcat cttcgctagc
      841 gagacgtaca cgattagcat cccggagcga gcggccgaat tcataatgag ctttgtcagc
      901 ctgaaatact actgaattct ctgccggagg cggcggaagt attgacaaac tgtctggtga
      961 agtttgttga gtgagaaaac cttctgcctt cagagcattt gctgagggga taaaaatcat
     1021 atttgtagca atacaaaaaa gaagaaagtt tttggttttc ataatttatt tccttagcat
     1081 aattaattga tgattttggt cagctgtaaa aaacgagctg tattaca
//
LOCUS       DAZXUK010000528         1109 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZXUK010000528 DAZXUK010000000
VERSION     DAZXUK010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.535"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1019
                     /locus_tag="ACH3L8_004455"
                     /pseudo
     CDS             1..1019
                     /locus_tag="ACH3L8_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            992..>1109
                     /locus_tag="ACH3L8_004456"
     CDS             992..>1109
                     /locus_tag="ACH3L8_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087247.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          312 a          271 c          258 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggtta
//
LOCUS       DAZXUK010000529         1102 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZXUK010000529 DAZXUK010000000
VERSION     DAZXUK010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.536"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            3..170
                     /gene="yecU"
                     /locus_tag="ACH3L8_004457"
     CDS             3..170
                     /gene="yecU"
                     /locus_tag="ACH3L8_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFT4087248.1"
                     /translation="MFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTTNFH
                     CNMTRKINEYA"
     gene            157..381
                     /gene="yecF"
                     /locus_tag="ACH3L8_004458"
     CDS             157..381
                     /gene="yecF"
                     /locus_tag="ACH3L8_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFT4087249.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(414..1034)
                     /gene="sdiA"
                     /locus_tag="ACH3L8_004459"
                     /pseudo
     CDS             complement(414..1034)
                     /gene="sdiA"
                     /locus_tag="ACH3L8_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
BASE COUNT          322 a          263 c          228 g          289 t
ORIGIN      
        1 aaatatttat tggtcattat attaacgtat tttatagcac tgccgatatc acgctcaaaa
       61 aacaaccact gctattttta gcaaagctta tggtatactc cgccgcctta acatttttca
      121 ccacaaattt tcattgcaac atgacgagga aaataaatga gtacgcctga tttttctact
      181 gccgagaata atcaagaact ggcaaatgaa gtctcctgcc tgaaagcgat gctgacgctg
      241 atgctgcagg cgatgggaca agctgacgcg ggccgcgtga tgttaaagat ggaaaaacag
      301 cttgcgctga tcgaagacga aacccaggct gcagtatttt ccaaaacggt taagcaaatt
      361 aaacaagcct accgtcagta atataaaacc ggctgagagc gtgcctttca gccggttatt
      421 gacaaaatca tcgctatctc tgctgatgtc ttcccttccg ccgtccattt cagaatttct
      481 ttttcgcgct tgctgaaatt catctctggc gtcatcacta tttcatcatt taaacgcatc
      541 agagccatca gactttcgcg caccagtaac tgcattttta attgcagttc atcactaaga
      601 atgggtattt cgcgcgtgct gcaacgggaa aaggacaaaa agcccagcgc ccgatttggc
      661 agcattaaat actgagtgac accgcggcgt aaaccatgtg cgtgcgcggc ttcccataat
      721 ggctgtgctt cgctgaataa gtcatcattc cacattaaat ggccctgact aaagttttca
      781 gggttcagca caggatcaat tgcgagaaaa ttttttgcct gataataact aacccacgac
      841 tcagggtaat tggtgtagaa agccacttta ggtcgcgtga atggtaccgg gtggcgaaca
      901 cataacgaat agtaatcgta ctccagctgc tgagcctgaa gctctatttc atggtaggcc
      961 tcttctgcgg cctccatcct ctgaaaacgc aacagcatcg tgcgacgcca gctgaaaaaa
     1021 tccgtatcct gcatagtaaa ccgcaacgcc cctgagagtg agtatcattt ataatgataa
     1081 ttcaaactat cacataaata ag
//
LOCUS       DAZXUK010000530         1099 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZXUK010000530 DAZXUK010000000
VERSION     DAZXUK010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.537"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..408)
                     /gene="sxy"
                     /locus_tag="ACH3L8_004460"
     CDS             complement(<1..408)
                     /gene="sxy"
                     /locus_tag="ACH3L8_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFT4087250.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
     gene            627..>1099
                     /gene="sulA"
                     /locus_tag="ACH3L8_004461"
     CDS             627..>1099
                     /gene="sulA"
                     /locus_tag="ACH3L8_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFT4087251.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQL"
BASE COUNT          302 a          260 c          241 g          296 t
ORIGIN      
        1 cacctccccg agaatcgctt ccagatgaaa agacatattg ggcaaatctt tcagtctttc
       61 ccgggtattg cgcgtgcttt tctctttcag cgctgcatcg agagaatatt tcgacagacg
      121 caccagcttc agttgatttc gccatagact ttcatcaacc cgatagtaat tgagggtaac
      181 ggatcggcca cactttttat atgtcagcca gacaggcgga tgttttacac agtactgtgc
      241 actttgctca caagcccgaa gatacaactc accatcagaa accatcgcaa acaccgtgtc
      301 gtcaacggtc aggctgtaac tgccaaacaa tgatcggtat tcaattgtgc ccaacgttgc
      361 caggtattct tgtgatttat agatccgctt ataggagagg cttttcataa aattcctttt
      421 aaaatcataa cataaaagaa tgattcacat taacggatcc gttaactacg aaaataggca
      481 acttattctt aaggggcaag attaatttat gttttcccgt caccaacgac aaaatttgcg
      541 aggctctttc cgaaaatagg gttgatcttt gttgtcactg gatgtactgc acatccatac
      601 agtaactcac aggggctgga ttgattatgt acacttcagg ctatgcacat cgttcttcgt
      661 cgttctcatc cgcagcaagt aaaattgcgc gtgtctctac ggaaaacact acagccgggc
      721 ttatcagtga agttgtctat cgcgaagatc agcccatgat gacgcaactt ctactgttgc
      781 cattgttaca gcaactcggt cagcaatcgc gctggcaact ctggttaaca ccgcaacaaa
      841 aactgagtcg ggaatgggtt caggcatctg ggctaccctt aacgaaagta atgcagatta
      901 gccagctctc cccttgccac acagtggagt caatggttcg cgctttacgc acgggcaatt
      961 acagtgtggt gatcggttgg ttgacagatg atttgactga agaagagcat gctgaacttg
     1021 ttgatgcggc aaatgaaggt aacgctatgg ggtttattat gcgtccggta agcgcatcct
     1081 ctcacacaac gagacaact
//
LOCUS       DAZXUK010000531         1097 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZXUK010000531 DAZXUK010000000
VERSION     DAZXUK010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.538"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..426)
                     /locus_tag="ACH3L8_004462"
     CDS             complement(<1..426)
                     /locus_tag="ACH3L8_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFT4087252.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIA"
     gene            complement(507..1031)
                     /locus_tag="ACH3L8_004463"
     CDS             complement(507..1031)
                     /locus_tag="ACH3L8_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFT4087253.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
BASE COUNT          312 a          231 c          204 g          350 t
ORIGIN      
        1 agcaataccc gctggccggt aaaacaactt aattctgttt tgcatcgcaa acttcagtgc
       61 attcttgcct tcttgctctg gactatttgg tggaatatac agaacattca gataaaaaat
      121 gctttcttta ttagtgggca gtttattcgg cataattttg atttttactt gctgcccgga
      181 atttgcccct atttttgcca ctggtggcgt taacatgaaa ggaacctgaa ttttttctgg
      241 tggtaacgac gtatcgccat catcaatcca cgcctgcagc agcgaagaac gatttccctg
      301 gttcatcaac tgcaccataa cttctttatt ttctgccggg taaataatgc gcgtcccgta
      361 gataacaatt ccggcatgtg caggaaggac catagaaaaa atgagtaaag ataataatcc
      421 tttcatctta atgcctctta aattaaaaag ccgccatggg gatagcggcc atgtttatta
      481 cctggagaaa cttgcaattt tatttattaa tcctgcgcca cacgcataac cagagaagat
      541 aacacatcac ctgattgcac tgagttatct gtcagtttca ggtattttgc tttaaacgga
      601 atagcatatt gaccttccgc tgttttataa ctctcaatgt taaaaggtgc agaaacatct
      661 aggacatcgc tgttatcacc tacggtatcg ttgctactgg ctatcgcctt atcgttaccg
      721 gttattttgt cgtttgtgat caacgagagt tttacaccag caacttcttt cgggcagcta
      781 atttttaaca tgctgctggt tacaccgact gcatcatcat aggtagaata tttagcttta
      841 ccttcaaaga cagtggttgg tacagtcggc atattaatac ttactgaatc accacccgct
      901 tcgaatttac acggagattc tgcaatattt cctttaatgg ttaatgttcc ggtatcaacg
      961 tctgcagcaa atactccagc gctcataaaa aaagaagaaa agacagcggc agcaataatt
     1021 gaacgtttca taatatgttc cttaataaaa tttatttttg gttgttaaga gtctgtatct
     1081 cttggaaaca aatttct
//
LOCUS       DAZXUK010000532         1091 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZXUK010000532 DAZXUK010000000
VERSION     DAZXUK010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.539"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACH3L8_004464"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACH3L8_004464"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFT4087254.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(986..1062)
                     /locus_tag="ACH3L8_004465"
     tRNA            complement(986..1062)
                     /locus_tag="ACH3L8_004465"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Asp,seq:gtc)
BASE COUNT          285 a          255 c          248 g          303 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     1021 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     1081 caccggtttt a
//
LOCUS       DAZXUK010000533         1077 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZXUK010000533 DAZXUK010000000
VERSION     DAZXUK010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.540"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(24..278)
                     /locus_tag="ACH3L8_004466"
     CDS             complement(24..278)
                     /locus_tag="ACH3L8_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFT4087255.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="ACH3L8_004467"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="ACH3L8_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFT4087256.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
BASE COUNT          274 a          258 c          279 g          266 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctatttt
//
LOCUS       DAZXUK010000534         1068 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZXUK010000534 DAZXUK010000000
VERSION     DAZXUK010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.541"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..560
                     /locus_tag="ACH3L8_004468"
     CDS             <1..560
                     /locus_tag="ACH3L8_004468"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFT4087257.1"
                     /translation="TKGDAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDN
                     YQAIQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKS
                     GLTGSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMV
                     NEVETALFNARALALKIYSMSGWNL"
     gene            787..>1068
                     /locus_tag="ACH3L8_004469"
     CDS             787..>1068
                     /locus_tag="ACH3L8_004469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HFT4087258.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          202 c          257 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DAZXUK010000535         1068 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZXUK010000535 DAZXUK010000000
VERSION     DAZXUK010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.542"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..244)
                     /locus_tag="ACH3L8_004470"
     CDS             complement(<1..244)
                     /locus_tag="ACH3L8_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFT4087259.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDILK"
     gene            310..1068
                     /locus_tag="ACH3L8_004471"
                     /pseudo
     CDS             310..1068
                     /locus_tag="ACH3L8_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          279 a          229 c          257 g          303 t
ORIGIN      
        1 tttttaatat gtcccgttcg tcggtaaccc gtttcagctc tttctggaga cggcggatct
       61 cggcctgagc atctgactgt tctttattag tggaagaatc cggaccgtac ttctttatcc
      121 aggcataaag gctgtgggtg gtgatatcga gacgtgttgc aacgctggca acagaataac
      181 cgcgatcaac aacctgtttg actgcttcag ttttaaactc ttcgggataa cgcttaccgc
      241 tcatgggcac ctctctttaa gccatcttaa atgactctga ggtgtctgtt aaacccgtgg
      301 cgattcagtg agcgcattct gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa
      361 gatacctctt atgcccgtgt aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact
      421 ggttacggtc agtttgaact agacctggaa gcatcaaaca gacacaaccc tgaccaaact
      481 cgtttggcat atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat
      541 gtcggtgtcg cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcggtgac
      601 tcatgggctg gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt
      661 aacacagatt tctttggcat ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa
      721 aatgaaggta caggcaacta caaagcaaat ggcgatggcc atggtttatc tgcaacttac
      781 actattgacg gtttctcatt tgctggtgct tatgcaaact ctgaccgtac tgattggcag
      841 tctggtgatg gtaaaggcga aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct
      901 aacaatgttt atgctgctgt gatgtacggt gaatctcaca acatgaattc cgacgacggt
      961 gatgttgtga acaaaacaca aaacttcgaa gctgttctgc aatatcagtt cgactttggt
     1021 ctgcgtccat ccatcggtta ctcctattct aaagcactgg acgtagca
//
LOCUS       DAZXUK010000536         1066 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZXUK010000536 DAZXUK010000000
VERSION     DAZXUK010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.543"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>1066
                     /gene="mxiD"
                     /locus_tag="ACH3L8_004472"
     CDS             <1..>1066
                     /gene="mxiD"
                     /locus_tag="ACH3L8_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFT4087260.1"
                     /translation="DHRYPIRGNISDKTFYISGPPALVELVANTATLLDKQVSSIGTD
                     KVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLLNNGKALSNRQAQNDPMPPF
                     NITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSILVKGNDQQIQIIRDIITQLD
                     IAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGASFNMSSSASISTLDGNKFI
                     ASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVSLVGERNSSLEHVTYGTLIN
                     VIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLPEVGRTKISTIARVPQGKSL
                     LIGGYTHETNSNEIISIPFLS"
BASE COUNT          359 a          163 c          211 g          333 t
ORIGIN      
        1 gatcacagat atcctataag aggaaacatt agtgataaaa cattctatat atctggtcct
       61 ccggcattgg ttgaactggt agctaataca gcaactttgc ttgataagca ggtttcttct
      121 attgggacag ataaagttaa ttttggggtt ataaaattaa aaaatacatt tgttagtgac
      181 agaacttata atatgagagg ggaggatatt gtcatcccag gggtggctac tgttgtagaa
      241 cgtttattaa ataatggtaa ggctctgagt aacagacaag cccaaaatga tccgatgcca
      301 ccctttaata ttacacagaa agtatctgag gatagtaatg acttttcatt tagttcagtt
      361 accaatagtt ctatattaga ggatgtatca cttattgcat atccagaaac caattctata
      421 ttagtcaaag gtaatgatca gcaaatacag attattcgcg atattatcac tcagttagat
      481 atagccaaac gacatataga attatcatta tggataatcg atattgataa atctgaatta
      541 aataaccttg gtgttaattg gcaaggaacg gcttcatttg gtgattcgtt tggtgcgtca
      601 tttaatatga gctcatcagc atcgataagc actttggacg gcaataagtt tattgcttca
      661 gttatggcat taaatcagaa aaagaaagca aatgtagttt caaggccggt gattttgaca
      721 caagagaata ttccggctat atttgataat aacaggactt tttatgtgtc attagttggt
      781 gagcgcaatt cttcgttgga gcatgttacg tatggaacat tgataaatgt tatacctcgc
      841 ttttcatctc ggggacaaat tgagatgtct ttaacgatag aggatggaac aggaaatagt
      901 cagtcaaatt acaattataa taatgaaaat acatctgtcc ttccagaagt tggaaggacc
      961 aaaatcagta caatagcgag agttccacaa ggcaaaagtt tgttgatagg tggttatacg
     1021 catgaaacga actctaacga aataatatct attccttttc tctcct
//
LOCUS       DAZXUK010000537         1065 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZXUK010000537 DAZXUK010000000
VERSION     DAZXUK010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.544"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACH3L8_004473"
     CDS             complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACH3L8_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFT4087261.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          299 a          322 c          249 g          195 t
ORIGIN      
        1 tttaatccta tctgagtgtt tagcattgtt gcgctccttt catgtgcaat gccatataaa
       61 gccactggcg gagggagcgc gcagggggca gccagtcgcc gccgccccct gcacccccgg
      121 gctctggcga acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt cggcaaccgg
      181 gtcgggcgcg aggtaacgtc ctgtaagacg cgccctcagc ccacatccat gtgggctgcc
      241 ccggccttcg gggaacgctt cagcgatttt gacgccacca atacccgtgc ggtttgtaat
      301 gtcgaagata aaacaaccag ggcagataaa atgccaatgc taattaatca cgcttttcca
      361 gcagagtccg gtaaatcaga ccaccgataa tgccgccgac aattggcacc acccagaaga
      421 accacagttg ttctaatgcc cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg
      481 ggttaacaga agtgttagtc accggaatac tgattaagtg aatcagggtt aaggccagac
      541 caatagcgat cggcgcaaaa cctgccggag cgaatttgtc ggttgcaccg tggatcacta
      601 acaggaagcc cgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc
      661 ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg
      721 ttttaccact ggcaattaaa tacagcagcg ccgctgcaac aataccgccg acaacctggg
      781 caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat aaaccaatag
      841 tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcgaaggcc atcgtcagaa
      901 cggtcaaacc aaatgccagc gccacgccgg caaaaccaat gcctaattcc gggaagcctg
      961 cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca aaacattcag
     1021 ctgctaattt tctgaacata tccacctcaa ttaaaaattg accct
//
LOCUS       DAZXUK010000538         1057 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZXUK010000538 DAZXUK010000000
VERSION     DAZXUK010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.545"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            19..885
                     /gene="ospB"
                     /locus_tag="ACH3L8_004474"
     CDS             19..885
                     /gene="ospB"
                     /locus_tag="ACH3L8_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFT4087262.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
BASE COUNT          388 a          154 c          183 g          332 t
ORIGIN      
        1 atatggagtc atgtaggtat gaatttagat ggtgttagac catactgtag aatagtcaat
       61 aaaaagaatg aaagcatatc agatattgca tttgcacata taataaaaag ggtaaaaaat
      121 tcatcatgta ctcacccaaa agcagcattg gtttttttag gagagaaagg tttttgtgat
      181 agcaatgatg ttctatctat tatgggacaa caaataccaa gagtatttaa gaacaagatg
      241 ttatatgatt atgtttttaa aaatgaaaaa agtaaaaatg attttctaaa aatggctgaa
      301 tcatggctac cacagagtga accaatagta ataaataatg atgatgacgc attgaatgct
      361 gctgcttatt tttctgtaaa aaaagcgaaa ataaaaacag taaacgatac tgattttaaa
      421 gagtataata aggtttatat tcttgggcac ggtagtcctg gttctcatca attaggcctt
      481 ggttcggaac ttattgatgt acaaacaatc atttcaagaa tgaaagactg tggtattcta
      541 aatgtgaaag atatccgttt tacttcatgc ggctccgctg ataaagtggc tcctaaaaat
      601 tttaacaatg cccctgctga aagtctttct tgtatcctta actctctgcc tttttttaag
      661 gaaaaagaat ctttgctaga gcagataaaa aaacaccttg aaaacgatga gtcattgagt
      721 gatggtctaa aaatatccgg ctatcatgga tatggagttc actatggtca agagcttttt
      781 ccctactcac attatcgttc aacttcaatt cctgctgatc cggagcatac agtaaaaaga
      841 agctctcaga aaaagacttt tattattaat aaagaactgg attagtataa aatttttaac
      901 ctatagttaa ttgtttgcat ttaagatgaa gttattttat ctttagtaga aaagtgcttc
      961 cctatgaggg aaaatcacaa atcatcataa tacagagaca aaacgatacg aaaaaataga
     1021 taaaaaacat cattctttta ccacattatt ttccgtg
//
LOCUS       DAZXUK010000539         1039 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZXUK010000539 DAZXUK010000000
VERSION     DAZXUK010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.547"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..466)
                     /gene="mxiE"
                     /locus_tag="ACH3L8_004475"
     CDS             complement(<1..466)
                     /gene="mxiE"
                     /locus_tag="ACH3L8_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFT4087263.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNG"
     gene            complement(596..1024)
                     /gene="mxiM"
                     /locus_tag="ACH3L8_004476"
     CDS             complement(596..1024)
                     /gene="mxiM"
                     /locus_tag="ACH3L8_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HFT4087264.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
BASE COUNT          345 a          220 c          120 g          354 t
ORIGIN      
        1 aaccatttac taaccgccaa gtgttcagtt gttctttgac ttttgcccct aaagcttttc
       61 tacataaaga tctaaaataa gcctccgaca caccataatg ctctgtcaga ctctttattc
      121 ttacctcctt ttctttctca atagttctca gaagataaag aaccaaatga tatgactcag
      181 tcattcgtat catagccagt gcattactta cattctggtc agatgaaaag atgaatctta
      241 acattaactt atcaagcatc gattcggtat tacaactatc ccgaagatca ttctcatgat
      301 agaaatgaat acatttttca gcatggctat gctctactag agcaccactg aaatagtccc
      361 aaaaagcaag aaattttgca caactattaa tgttaaagcg ataatgatag ttcacattat
      421 cactgaattt tatattttga tttcggacaa aaaaaaaccc ttccatgctg gagccagctc
      481 aattatgtta cgttctttat cttttacaag ctcaacgtta actggttcat gtccactaga
      541 gtaaatgtaa aacatattac ttgtatttag acctttatat ttactcacaa tacaattata
      601 tccctcgaat taattttaat ttaacttccc catccttctc tataaggatg gtatcattta
      661 tcttattacc ttttaaaaca accccgtctt tcagcataga aaatagttta tctatagcta
      721 tcttttgtgc atccatgaag cacccatcta cttccttaac attaaagttg cccatgaatt
      781 tacctgcatt aaaactataa accgccttac cactaataca tttagagtaa acatttatac
      841 ttttattggt tgtattaaac gaccataata aatcatttgg aatagaaaaa taatccttac
      901 ttacaggaac aatatgccat tctttttcag aattagatga tgactttaaa gcacacccac
      961 tcagtgttaa tagcaatata cttaaaataa atattttcaa cttattacta ccatgtcgaa
     1021 tcatctgcct ctctccctg
//
LOCUS       DAZXUK010000540         1036 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZXUK010000540 DAZXUK010000000
VERSION     DAZXUK010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.548"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..199)
                     /locus_tag="ACH3L8_004477"
     CDS             complement(<1..199)
                     /locus_tag="ACH3L8_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFT4087265.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFS"
     gene            445..>1036
                     /locus_tag="ACH3L8_004478"
     CDS             445..>1036
                     /locus_tag="ACH3L8_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFT4087266.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQI"
BASE COUNT          377 a          210 c          169 g          280 t
ORIGIN      
        1 atgaaaatct ctccaaaacg ctatcaatga ttgagttcct gtcaaccgct ccaccattac
       61 tttccagtaa cactgccaaa atacaccccg ccgtagctgg aaaatttatc tcatcctggg
      121 tatttacatt cgagagcgag catttgttcg gatcaaaaat aatcgcgtca ttgataatga
      181 aaaaacatct ccgctccatg caggcaacaa cacaaaataa gattttgtca ttttagccta
      241 ttcctggtgg aactgacaca agaaactaaa atacaaatat aaccctgaaa aagtcatata
      301 aaacttatat ccacagaata aaaaatcaaa catgtcagtt ttatcaatat aaagtatcgc
      361 aaggcgaaaa gtgatgcaag aatactccta agtaaatgaa agcgcaccac atgaaattat
      421 caacacatgt aatttcatca ttaggtgcta tcgaaaataa ctcaggagct acttaagatg
      481 aataatgtct tactcatgag acccgatgac tcagaattta tgatacctga tgactggtca
      541 gggctaaacg caaaaggcat tacatacgaa caagataata gagtgacaga gtcttttctg
      601 ccttttaaat ctttttattg ttcaccggtg tgcacgacag ttgaactcat ctcacaaaat
      661 aattgttttg ctatgggtat ccgctacctt ctggaaaaga aaagagactt aaggctaaat
      721 cactttaaag ccttctctca taaatgcgag acaaaacctg acgtgttaat tatgaccggg
      781 tgcaccgtcg ctgatactta tagtatattc tcacgcattg ttgaagaaaa tataatgaaa
      841 acgatgacac ctatcgtcat cattactggg catcactcag aagatctttg ttctctggca
      901 tcgaaactgg gaggagttaa aataatatca ccgacagaaa agattgcaaa aatagaaaac
      961 gctatcaatg atgcttttaa cagcaagcct aaaaaaataa gatttttaac agatatactc
     1021 tctcctcaac aaattg
//
LOCUS       DAZXUK010000541         1033 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZXUK010000541 DAZXUK010000000
VERSION     DAZXUK010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.549"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            208..468
                     /gene="mcbA"
                     /locus_tag="ACH3L8_004479"
     CDS             208..468
                     /gene="mcbA"
                     /locus_tag="ACH3L8_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFT4087267.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            733..>1033
                     /locus_tag="ACH3L8_004480"
     CDS             733..>1033
                     /locus_tag="ACH3L8_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFT4087268.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT          298 a          257 c          215 g          263 t
ORIGIN      
        1 tatcactccc caaaacctta acatggctat ggtttatggc aattcatcat aaaaatcagc
       61 attatgcatg tcattgattt gtcttatttt gtaaaaatat atcgttcaaa aaataagcat
      121 ttctctgtca attttttgcg tcatttgcat cgcgcaggcg acctacactt atcaggcact
      181 acccgttgta tttatgagga tgtcattatg aaaaagtgcc tcacactact gattgccacc
      241 gtcctgagcg gaatctctct cacggcttat gccgcgcaac cgatgagtaa cctggacagc
      301 ggtcagctac ggcccgccgg taccgtttcg gcaactggcg catcaaacct aagcgatctg
      361 gaggataaac tggcagaaaa agcgcgcgaa caaggcgcga aaggttatgt cattaattcc
      421 gcaggcggaa atgaccagat gttaggtact gcaaccatct acaaataacc ccaccaacgt
      481 aactgcattc gcctctggat gcagttttcg cctgttttgt aatcaaaaaa catcaaatgt
      541 gaacgcaatg taaataaatg tatttctttt tcgcgcaatg ggtgatagaa aatcgcatca
      601 agtgataatg cttatcaaaa ttattatcac ttgcacgaac actatcacgg gattaacagt
      661 ggcatcgcat ccgcagagat gctttctcgt ggcagtgaaa atttcaacat ataagaaaaa
      721 gtcacctgca aaatggaaaa caatcgcaat ttccctgcca gacaatttca ttcgctcacg
      781 ttctttgccg gtctttgtat tggtattacg cctgtggcac aggcactcgc cgccgaaggg
      841 caagctaacg cggatgacac gctggttgtc gaagcatcaa cgccttcgct ttatgcgcca
      901 caacaatctg ccgatccgaa attctcgcgt ccggtagcgg atactacccg cacgatgacg
      961 gtgatttctg aacaagtgat taaagatcag ggcgcaacca accttaccga cgcgctcaaa
     1021 aacgtccccg gcg
//
LOCUS       DAZXUK010000542         1029 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZXUK010000542 DAZXUK010000000
VERSION     DAZXUK010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.550"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>1029)
                     /locus_tag="ACH3L8_004481"
     CDS             complement(<1..>1029)
                     /locus_tag="ACH3L8_004481"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFT4087269.1"
                     /translation="MYQVAQRTNFPNRIVCIKNPGYSNYTFVNDNCSIHRVGFSRIYK
                     RLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNSMKLYTQIRKLAPQARVVIHM
                     HNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPNGIDLETYQSNFQPITRSE
                     LNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLKLVVIGDYTEMSKSDKGAY
                     QRNVREIAKRLKDRCIMLGSIPPEKMYCYYPLADLVVIPSQFQEPFCMVAIEAMGAGK
                     PVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTLANPELTAVAKQGQDFVFD
                     HYSWDGVT"
BASE COUNT          313 a          208 c          172 g          336 t
ORIGIN      
        1 gagttacgcc atcccaactg tagtgatcaa acacaaagtc ctgaccttgc tttgcaactg
       61 ctgttaattc aggattcgct aacgttttta aaatatcgct gcttatggag tctgcagtca
      121 taggttcttt taaatggaat cccgtagtat tttctttgac aaattccgtc atccccccac
      181 gcgtactcac taaaacaggc ttaccagcac ccatagcttc aattgccacc atgcaaaatg
      241 gttcctggaa ctgggaagga atgacgacta aatctgccag tgggtaataa caatacatct
      301 tttcaggcgg tatgcttcct aacataatgc aacgatcctt caaacgtttt gcaatttccc
      361 tgacattgcg ttgatatgca cctttgtcac ttttactcat ttctgtataa tcaccaatca
      421 caactaattt taaatttttg tgagcagcgg ccaatttttc gaatgattgc atcaacagca
      481 atatgccttt gtcagggacg atacgcccag cataaaaaat aattttctct tcaggagaga
      541 tattaagttc gcttctagtg atcggctgaa aattagattg ataggtttct aaatcaattc
      601 catttggtac aatctcaata tctgcatttg ctaaataaga ttggtaatac tttttcaagt
      661 ataaactagg gactatcatt ttaacatttt gctcaagcaa ttttggttca aatgcattat
      721 gcatatgtat aaccactctt gcctgaggag ctagcttacg aatttgagtg tatagcttca
      781 tgctattatg aactacaata acactttctt tcgttatagg aaaatcatgt gcaatattca
      841 atattctctg tgaatatgga agaggatcaa gtcttgtcca tttttgaaat aaccgcttat
      901 atatgcgact gaatccaacc ctatgaatac tacaattatc attaacaaag gtgtaatttg
      961 agtatcctgg atttttgata caaacaatac gatttggaaa attggtacgc tgagcaacct
     1021 gatacatcc
//
LOCUS       DAZXUK010000543         1009 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZXUK010000543 DAZXUK010000000
VERSION     DAZXUK010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.551"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..632
                     /gene="ospI"
                     /locus_tag="ACH3L8_004482"
     CDS             <1..632
                     /gene="ospI"
                     /locus_tag="ACH3L8_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFT4087270.1"
                     /translation="GVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSELAA
                     TLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQIDSE
                     GIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLDVQT
                     GKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            747..1009
                     /locus_tag="ACH3L8_004483"
                     /pseudo
     CDS             747..1009
                     /locus_tag="ACH3L8_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          311 a          156 c          251 g          291 t
ORIGIN      
        1 atggggtgtc gttacaggga actgctggat atgaggcaca tacagaggag ggaaatgtaa
       61 atgttaaaaa attgctggaa tctttaaact ctaaaagtct tggtgatatg gataaagatt
      121 ctgaattggc agcgacttta cagaaaatga taaatccgtc tggtggggat ggtaactgtt
      181 cagggtgtgc gcttcatgct tgtatggcta tgttagggta tggagtaaga gaggctcccg
      241 tacctaatga aatatcagag tatatgaccg gattttttca taggcattta gagcaaattg
      301 atagtgaagg catagtgtca catcctaatg aaacttatag taaatttcgt gagcgtattg
      361 cagaaaatat tttacaaaat acttcaaaag gaagtgtagt aatgatttct attgaacagg
      421 ccactcattg gatagctgga tttaacgatg gtgaaaaaat tatgtttttg gatgttcaaa
      481 caggaaaggg atttaatctt tatgatcctg tagaaaaatc gccagatgca tttgtagatg
      541 aaaatagttc ggttcaggta attcatgtat ctgatcagga atttgatcac tatgctaaca
      601 gtagtagttg gaaaagtaag aggctttgct gatgctgaga gcctgtacat agatttgagc
      661 cggccccttg aaacgccaga cagtggatgt atcttaaaat aagagatagg cttggatata
      721 tgtctaacac tagttctaat tttgagatga ctggaacttt gctggggctg gagcttcgta
      781 aacgtaaaac tcctcaggaa aaaattgcca ttattcagca gacaatggag cctggcatga
      841 cggtgtccca tgtggcgcgt ttgcatggta ttcagcccag cctgttgctt aagtggaaaa
      901 aataatatct ggaaggaagt ctcactgctg ttgctgccgg agaggatgtc gttcctgcct
      961 cagaactggc tgccgctata aaacaaataa aagagttaca gcgcttgct
//
LOCUS       DAZXUK010000544          998 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZXUK010000544 DAZXUK010000000
VERSION     DAZXUK010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.552"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..167
                     /locus_tag="ACH3L8_004484"
     CDS             <1..167
                     /locus_tag="ACH3L8_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFT4087271.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="ACH3L8_004485"
     CDS             152..790
                     /locus_tag="ACH3L8_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFT4087272.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
BASE COUNT          272 a          191 c          291 g          244 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataa
//
LOCUS       DAZXUK010000545          992 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZXUK010000545 DAZXUK010000000
VERSION     DAZXUK010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.553"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..264
                     /locus_tag="ACH3L8_004486"
     CDS             <1..264
                     /locus_tag="ACH3L8_004486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087273.1"
                     /translation="RHVKRFCFFSTPPWTIFYCKKTRFVTACAQDISQQSATGKKNDC
                     LVIFDSGKRRFKRKQNIFCVDNSVFTRVLILWYEQKKKNTTRG"
     gene            393..848
                     /gene="csgB"
                     /locus_tag="ACH3L8_004487"
     CDS             393..848
                     /gene="csgB"
                     /locus_tag="ACH3L8_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFT4087274.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            889..>992
                     /locus_tag="ACH3L8_004488"
     CDS             889..>992
                     /locus_tag="ACH3L8_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HFT4087275.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
BASE COUNT          309 a          177 c          237 g          269 t
ORIGIN      
        1 cgtcatgtaa aacgtttttg ttttttctcc acacctccgt ggacaatttt ttactgcaaa
       61 aagacgaggt ttgtcacggc ttgtgcgcaa gacatatcgc agcaatcagc gacgggcaag
      121 aagaatgact gtctggtgat ttttgatagc ggaaaacgga gatttaagag aaaacaaaat
      181 attttttgcg tagataacag cgtatttacg cgggttttaa tactttggta tgaacaaaaa
      241 aagaaaaata caacgcgcgg gtgagttatt aaaaatattt ccgcagacat actttccatc
      301 gtaacgcagc gttaacaaaa tacaggttgc gttaacaacc aagttgaaat gatttaattt
      361 cttaaatgta cgaccaggtc cagggtgaca acatgaaaaa caaattgtta tttatgatgt
      421 taacaatact gggtgcgcct gggattgcag ccgcagcagg ttatgattta gctaattcag
      481 aatataactt cgcggtaaat gaattgagta agtcttcatt taatcaggca gccataattg
      541 gtcaggctgg gactaataat agtgctcagt tacggcaggg aggctcaaaa cttttggcgg
      601 ttgttgcgca agaaggtagt agcaatcggg caaagattga ccagacagga gattataacc
      661 ttgcatatat tgatcaggcg ggcagtgcca acgatgccag tatttcgcaa ggtgcttatg
      721 gtaatactgc gatgattatc cagaaaggtt ctggtaataa agcaaatatt acacagtatg
      781 gtactcaaaa aacggcagtt gtagtgcaga gacagtcgca aatggcaatt cgcgtgacac
      841 aacgttaatt accattcgac ttttaaatca atccgatggg ggttttacat gaaactttta
      901 aaagtagcag caattgcagc aatcgtattc tccggtagcg ctctggcagg tgttgttcct
      961 cagtacggcg gcggcggtaa ccacggtggt gg
//
LOCUS       DAZXUK010000546          991 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZXUK010000546 DAZXUK010000000
VERSION     DAZXUK010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.554"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            24..941
                     /locus_tag="ACH3L8_004489"
     CDS             24..941
                     /locus_tag="ACH3L8_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087276.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
BASE COUNT          315 a          203 c          209 g          264 t
ORIGIN      
        1 ttttaagcag tcggagaaag aggatggttc agaaaatttt gtcggataag gttatgaatg
       61 aaagaacaaa tgcgtattac tcttattatc ttggagagcg taatatctcc gtactgcccc
      121 ttaatgttta tgatcccccg gaacgtttta tcgcttacat aaaaaaaaac agagaaaatc
      181 tgaacatcac actttcagat tttgaacttg aacaaataat ttctggtatg cgtctgaagg
      241 ccctggcctt cctggtcccc ctggaaaaaa tatcctggat cgcgggcagt gaacgcgcat
      301 gcttattttc ctggtatctg ttgatgcagt ttatccagaa taacagggca aaaataagcg
      361 cagacttgct tcaaaagaat aaactgtatc ttaaagaaga gtatctggaa gggaatgcct
      421 tcccgtcgga ctcatcgaca cagttcagac aaatactcag agttctcgat attctttctg
      481 ataagaattt gcgggatgag tggataatcc agaccaaaga cagatggatg cgggcattta
      541 aatcaaagag tcctttcagt tacctcctcc cggagaacga acatgaatgc atatggacat
      601 ggaattattt gaagggaaaa aatattgcac tggaaaaact ggccagtttt cctggttctg
      661 ctgatattta ccacgcaatt cacctttcat ttgacatatg ggttacctgt ccactcacct
      721 cacctgacga tataaaaaat ttcagaaaca gtttcaataa ggccaaggcg cagcgcaagt
      781 acaaaaaaat gcaggaagat aaagtcaatg tacagttttt tcttgacgca gaaaccaggg
      841 cgcagctcaa agaattatcg agggtcagaa gactcagtac cggcgaaatg ctgcatgacc
      901 tgattgttga agagtataaa aggtaccgac attccagata atttcagaca tatattatct
      961 ccgtgagtcc cccgccacct ttccggtgtg c
//
LOCUS       DAZXUK010000547          985 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZXUK010000547 DAZXUK010000000
VERSION     DAZXUK010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.555"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..158)
                     /locus_tag="ACH3L8_004490"
     CDS             complement(<1..158)
                     /locus_tag="ACH3L8_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFT4087277.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="ACH3L8_004491"
     CDS             complement(308..469)
                     /locus_tag="ACH3L8_004491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087278.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(469..654)
                     /locus_tag="ACH3L8_004492"
                     /pseudo
     CDS             complement(469..654)
                     /locus_tag="ACH3L8_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            914..>985
                     /locus_tag="ACH3L8_004493"
     CDS             914..>985
                     /locus_tag="ACH3L8_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFT4087279.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSK"
BASE COUNT          295 a          256 c          180 g          254 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaa
//
LOCUS       DAZXUK010000548          982 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZXUK010000548 DAZXUK010000000
VERSION     DAZXUK010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.556"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(2..133)
                     /locus_tag="ACH3L8_004494"
     CDS             complement(2..133)
                     /locus_tag="ACH3L8_004494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087280.1"
                     /translation="MARSAKSRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFD"
     gene            215..408
                     /locus_tag="ACH3L8_004495"
                     /pseudo
     CDS             215..408
                     /locus_tag="ACH3L8_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
     gene            551..772
                     /locus_tag="ACH3L8_004496"
                     /pseudo
     CDS             551..772
                     /locus_tag="ACH3L8_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(793..855)
                     /locus_tag="ACH3L8_004497"
                     /pseudo
     CDS             complement(793..855)
                     /locus_tag="ACH3L8_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          293 a          234 c          227 g          228 t
ORIGIN      
        1 ttcaatcaaa gcgcagaact tctgcgtctt cttcgtcaaa aaaatcatct tcgtgaagct
       61 tcacgacata gcggggaagt ttgcttcttt gagaggcggg tttacgttta cgggatttag
      121 ctgaacgggc catataacca cctgaaagac aatgacattg cctgttttta taacggtaat
      181 tgcagaccat gacaaaccgc agccgtcagg ctgcctactc gagtcacaag gctctttgtg
      241 tccgatatgc cggcagcgca ttacccatga aactggctgg aacgtacacc ataaggtgaa
      301 gaaagttatg ggggcggtga agaattatct aaccttgtct tgttacatcc aaattgccat
      361 cgacagttac acagttgtga agccggctct ctcaaaaagg gcttataaag gcttgagcgg
      421 tgtaccggga aaccggtatg caccgttctt aggggagggc agtccagcaa tgaactgtcc
      481 ttacccgaca aacatccaaa acgaacggaa cgtgctggaa tccgcatata acccgttgta
      541 aagtgaggaa atggacacct cacttgctca tgagaacgcc cgcctgcggg cactgttgca
      601 gacgcaacag gacactatcc accagatggc tgaatacaac cgcctgctct cacagcggat
      661 ggcggcttat gcttccgaaa tcaaccgact gaaggcgctg gttgcgaaac tgcagcgtat
      721 gcagttcggt aaaagctcag aaaaacttcg cgcaaaaacc gaacggcaga ttccattttc
      781 acgggcgatc tacgccacac aggctctggg ttgcagtgac tgctccatta aggcgatttt
      841 gaactcataa gggaagtaac aaaaaaactg caaaagtaca ataattatat atggccaaac
      901 taacctctat taaaaaacat cagtttttca cacttagttg agcttcatga aaaaatcatc
      961 gaggatttca ttgcagaata ca
//
LOCUS       DAZXUK010000549          965 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZXUK010000549 DAZXUK010000000
VERSION     DAZXUK010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.558"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(48..560)
                     /locus_tag="ACH3L8_004498"
     CDS             complement(48..560)
                     /locus_tag="ACH3L8_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087281.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(699..>965)
                     /locus_tag="ACH3L8_004499"
     CDS             complement(699..>965)
                     /locus_tag="ACH3L8_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFT4087282.1"
                     /translation="SGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGIAYV
                     SGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT          266 a          232 c          180 g          287 t
ORIGIN      
        1 aaaggcggga tatcaaatcc cgccatgaaa ttaactaatc atctgcatta ttctgcgtag
       61 atataagcct gggtacggat ggtgtaagag tctggctcaa caacctgttc tttcatatgg
      121 gaaacgacgc gataggcacc acctgttcca gtatttttca gcatcatata acgcttaccg
      181 ccaatctctt cggaggcagc tgaaggagga tcctgctcaa gcgttgtcag ggaaactttc
      241 agtttatggt ccgggttatt gtcaccagaa attactgcat ccgctgaagt actgctctcc
      301 gcataaatat catccgggaa agttacagca tagtcgtggc tctgcccatc agatgcgcca
      361 acaatacctg ttgccaaagg cgtccatgca gttaacgtac cgctatatac ccctttcact
      421 ggttgaatgg tattattaac aacgataggc ttggtcaaag taagattagc tgacgcttca
      481 gtaccctggc ttactaaagt tcccgcctga gaagcaaaag ctgtccccgc cagcatgatt
      541 gctaacaatg ttttcttcat tatattttct ccatgatttt catttacact gcaaaatgca
      601 gtttctttct ttgcatttac gtgctatcgc gaaaatgctt ccaccaaata atggaaacat
      661 cttcacaaaa ataaattatg agtttcttaa tttagaaact ataaacatgt cgccgctatc
      721 tggtataacc ctgatgcact tttcttcgca ggaagttggt attttgcgat acaggattga
      781 tctttagaat aaccccattt cacattaaac tcgccttccg cactcaagcc agaaacataa
      841 gcaataccat cttcacctac aatgccggcc atattttcag tgtctttgct ataactaatc
      901 acagcgccga aggggatggg tttaccctct ttctgtttta aggttactaa tcctttatcc
      961 ccgct
//
LOCUS       DAZXUK010000550          952 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZXUK010000550 DAZXUK010000000
VERSION     DAZXUK010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.559"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..839
                     /gene="ospC2"
                     /locus_tag="ACH3L8_004500"
     CDS             <1..839
                     /gene="ospC2"
                     /locus_tag="ACH3L8_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFT4087283.1"
                     /translation="HTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFL
                     DNFKEVVDEVSRYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNK
                     NIDPVSLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTD
                     KKMAHQALAYSLGNAKSDMALYLLSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSED
                     SANYKVLEYFISNGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRF
                     GR"
BASE COUNT          332 a          130 c          188 g          302 t
ORIGIN      
        1 atcatactgt ggattttggt gctaatgcgt atatcattga tcatgactct ccatatggat
       61 atatgacatt aaccgaccac tttgataatg ctattccacc tgttttttac catgagcacc
      121 aatcattttt cttagataac tttaaagagg ttgtggatga agttagcagg tatgtgcatg
      181 gtaatcaggg aaaaactgat gtgccaatat ttaatactaa agatatgagg cttggaattg
      241 ggttgcatct tattgatttt ataagaaaga gtaaagatca aagattccga gagttttgtt
      301 ataataaaaa cattgatcct gttagtctgg atagaattat aaactttgtg tttcagcttg
      361 agtatcatat accaagaatg ctaagcacag ataacttcaa aaagataaga ctcagagata
      421 tatctttgga ggatgccatt aaagcatcca attatgaaga aattaacaat aaggtgactg
      481 ataaaaaaat ggctcatcaa gctcttgctt attctctagg caatgcaaaa tctgatatgg
      541 cactttattt actatctaaa tttaatttta caaaacaaga tatcgcagag atggagaaaa
      601 tgaacaacaa tatgtattgt gagctgtatg atgttgagta tttacttagt gaagatagtg
      661 ccaactataa agtgctagaa tattttatta gtaatggatt ggttgatgta aacaaaagat
      721 ttcaaaaagc aaatagtggg gacactatgc ttgacaatgc aatgaaaagc aaagattcaa
      781 aaacaattga ttttttatta aaaaatggag cggtatcagg caaacgattt gggaggtgac
      841 ctaaaatata tttaggttgt gccctgaatc tgacgttgtc atttttgtta tattgatcga
      901 tataggggat ggtgcagacc gttcccctgc aacaacacat tgcatgtggt ga
//
LOCUS       DAZXUK010000551          947 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZXUK010000551 DAZXUK010000000
VERSION     DAZXUK010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.560"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..906
                     /gene="leuO"
                     /locus_tag="ACH3L8_004501"
     CDS             <1..906
                     /gene="leuO"
                     /locus_tag="ACH3L8_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFT4087284.1"
                     /translation="LSKPQLRMVDLNLLTVFDAVMQEQNITRAAHVLGMSQPAVSNAV
                     ARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPGSGFEPASSERVFH
                     LCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQETEFVISYEDFHR
                     PEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDRFASFSQPWYDTVD
                     KQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVLPLPLKQNSRTCYL
                     SWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          252 a          207 c          222 g          266 t
ORIGIN      
        1 ttaagcaagc cacagctacg catggtcgat ctcaacttat taaccgtttt cgatgccgtg
       61 atgcaggagc aaaacattac ccgtgccgct catgttctgg gtatgtcgca acctgcggtc
      121 agtaacgctg ttgcacgcct gaaggtgatg tttaatgacg agctttttgt tcgttatggc
      181 cgtggtattc aaccgactgc tcgcgcattt caactttttg gttcagttcg ccaggcattg
      241 caactagtac aaaatgaatt gcctggttca ggttttgaac ccgcgagcag tgaacgtgta
      301 tttcatcttt gtgtttgcag cccgttagac agcattctga cctcgcagat ttataatcac
      361 attgagcaga ttgcgccaaa tatacatgtt atgttcaagt cttcattaaa tcagaacact
      421 gaacatcagc tgcgttatca ggaaacggag tttgtgatta gttatgaaga cttccatcgt
      481 cctgaattta ccagcgtgcc attatttaaa gatgaaatgg tgctggtagc cagcaaaaat
      541 catccaacaa ttaaaggccc gttactgaaa catgatgttt ataacgaaca acatgcggcg
      601 gtttcgctcg atcgtttcgc gtcatttagt caaccttggt atgacacggt agataagcaa
      661 gccagtatcg cgtatcaggg catggcaatg atgagcgtac ttagcgtggt gtcgcaaacg
      721 catttggtcg ctattgcgcc gcgttggctg gctgaagagt tcgctgaatc cttagaatta
      781 caggtattac cgctgccgtt aaaacaaaac agcagaacct gttatctctc ttggcatgaa
      841 gctgccgggc gcgataaagg ccatcagtgg atggaagaac aattagtctc aatttgcaaa
      901 cgctaactga atgcagaata ggccagacat gaatgtctgg tttattc
//
LOCUS       DAZXUK010000552          936 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZXUK010000552 DAZXUK010000000
VERSION     DAZXUK010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.561"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            144..641
                     /locus_tag="ACH3L8_004502"
                     /pseudo
     CDS             144..641
                     /locus_tag="ACH3L8_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            682..873
                     /locus_tag="ACH3L8_004503"
                     /pseudo
     CDS             682..873
                     /locus_tag="ACH3L8_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          244 a          232 c          269 g          191 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcaaagcgcc agccccgtcc cgcccgatag agcgcggcat
      121 agcgggcccc ggattacttg cgtgcgacgg cagaagaagc cctgaagcga atcagtgaac
      181 tgtacgccat cgaggatgaa atacggggat taccagagtc agagtgtctt gcagtcaggc
      241 agcagcgaag caaagcgtta ctgacgtcgc tgcatgaatg gatggtggag aagaatggca
      301 cgctgtcgaa aaaatccaga ctgggcgaag cattcagcta tgtactgaat cagtgggacg
      361 ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca gcggaaagag
      421 cgcttcgtgc agtctgtctc ggaaagaaaa attacgtgtt cttcggtagc gatcacggcg
      481 gcgagcgtgg tgcactgctg tacgggctga tcggcacctg ccgtctgaac ggtatcgatc
      541 cggaagcgta tctgcgccat attctgagcg tactgccgga atggccttcc aaccgagttg
      601 acgacctcct gccatggaag gtggtgctac catcaggata atcgtcaata cggtgttcgg
      661 tgaacgttta cttggagcat catgcttcat gaagaaaaac tggcacgtca tcaacgtaaa
      721 caggcgatgt atacccgaat ggcagccttc ccggcggtga aaatgttcga agagtatgac
      781 ttcacgttcg ccaccggagc accgcagaag cagctccagt cgttacgctc actcagcttc
      841 atagagcgca atgaaaacat agtcttactg gggacatctg acatcacaaa tccccgggtg
      901 gggatttgtg tataagagac agcccatgga ggaagg
//
LOCUS       DAZXUK010000553          935 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZXUK010000553 DAZXUK010000000
VERSION     DAZXUK010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.562"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..158
                     /locus_tag="ACH3L8_004504"
     CDS             <1..158
                     /locus_tag="ACH3L8_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFT4087285.1"
                     /translation="ALRDPLSRAYYTRKMSQGKRHNQVLIALARRRCDVLFAMMRDGT
                     FYTPQGS"
     gene            185..532
                     /locus_tag="ACH3L8_004505"
                     /pseudo
     CDS             185..532
                     /locus_tag="ACH3L8_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
     gene            529..624
                     /locus_tag="ACH3L8_004506"
                     /pseudo
     CDS             529..624
                     /locus_tag="ACH3L8_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          263 a          234 c          212 g          226 t
ORIGIN      
        1 ccgcgctcag ggatccgctc tccagggctt actacacccg caaaatgagt cagggaaaac
       61 gacacaatca ggtgcttatc gccctggcga gacgacgctg cgacgttctg ttcgccatga
      121 tgcgcgacgg gactttttat accccgcagg ggtcataaca tgcttgacaa cttaataggg
      181 gcactccccc gctaaagtgt tgcccgaaaa ctggtggcaa catccagctg cattaggtgc
      241 aactgacagt gatatcgaaa tcatcaaacg tcagtggggt gcgttttacg gtacggatct
      301 ggagttgcaa ctgcgccgcc gaggtatcga tacaatagtg ttatgtggga tctcgaccaa
      361 tatcggtgtt gaatccaccg cccgcaatgc ctgggaactt ggctttaatc tggtgattgc
      421 cgaagacgcc tgtagtgccg ccagcgccga gcaacacaat aacagcatta atcatatcta
      481 cccgcgcatc gcccgtgtgc gtagcgtgga agagatcctt cacgcgttat gatttacatc
      541 ggtctaccgc aatggtcaca tcctaaatgg gtgcggttgg ggatcaccag ccttgaagag
      601 tatgcccgcc actttaactg cgtgatacgg gatttttaaa aatcactaaa gaacgcccaa
      661 gagcatgtgt tttctttaga ataatcaaag cgttggattg ccatgtcata tgaaaatcgg
      721 caaagttcat atgtaacaaa gtgtcccaat catgccccaa aatgccccac aacaaatatt
      781 tttgccccat gcatgcccca aaaaatcact ttccctcact cttgacactg tttatccata
      841 cagttaaaaa taatactgta taaaaacaca gtgcagaggg atttttatgc gttttgaaat
      901 ctgcatagcc aaagagaaaa tgactaaaat gccaa
//
LOCUS       DAZXUK010000554          931 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZXUK010000554 DAZXUK010000000
VERSION     DAZXUK010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.563"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            168..764
                     /gene="fimE"
                     /locus_tag="ACH3L8_004507"
     CDS             168..764
                     /gene="fimE"
                     /locus_tag="ACH3L8_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFT4087286.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          270 a          169 c          205 g          287 t
ORIGIN      
        1 gttgtcacat cagtgatatt ttatttttgt atgatgttta atgtaattga ctgatagcca
       61 cataacttcg tgtgtggtta tctttttatc tattgggcta attttgaccg attgaggttt
      121 cctataggta ttcattcaaa tatatctcag ttaggagtac tactattgtg agtaaacgtc
      181 gttatcttac cggtaaagaa gttcaggcca tgatgcaggc ggtttgttac ggggcaacgg
      241 gagccagaga ttattgtctt attctgttgg catatcggca tgggatgcgt attagtgaac
      301 tgcttgatct gcattatcag gaccttgacc ttaatgaagg tcgaataaat attcgccgac
      361 tgaagaacgg attttctacc gttcacccgt tacgttttga tgagcgtgaa gccgtggaac
      421 gctggaccca ggaacgtgct aactggaaag gcgctgaccg gaccgacgct atatttattt
      481 ctcgccgcgg gagtcggctt tctcgccagc aggcctatcg cattattcgc gatgccggta
      541 ttgaagctgg aaccgtaacg cagactcatc ctcatatgtt aaggcatgct tgcggttatg
      601 aactggcgga gcgtggtgca gatacccgtt taattcagga ttatctcggg catcgaaata
      661 ttcgccatac tgtgcgttat accgccagta atgctgctcg ttttgccgga ttatgggaaa
      721 gaaataatct cataaacgaa aaattaaaaa gagaagaggt ttgatttaac ttattgataa
      781 taaagttaaa aaacaaataa acacaagaca attggggcca aactgtccat atcataaata
      841 agttacgtat tttttctcaa gcataaaaat attaaaaaac gacaaaaagc atctaactat
      901 ttgatatgta aattatttct cttgtaaatt a
//
LOCUS       DAZXUK010000555          928 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZXUK010000555 DAZXUK010000000
VERSION     DAZXUK010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.564"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>928)
                     /locus_tag="ACH3L8_004508"
     CDS             complement(<1..>928)
                     /locus_tag="ACH3L8_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFT4087287.1"
                     /translation="LMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPLEENTTFRS
                     QAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEAPETALTFL
                     SKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLVAYQIVPDK
                     AISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMFDKAIAVDS
                     SRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEGILLFYRNN
                     DMQQDYQNSLSNYIVFIDKIFAFSPSTRSELCL"
BASE COUNT          280 a          162 c          179 g          307 t
ORIGIN      
        1 gaggcataat tcacttcttg ttgatgggga aaaggcaaaa attttatcga taaatactat
       61 gtaatttgaa aggctgtttt gatagtcttg ctgcatatca ttatttctat aaaacaataa
      121 aattccttcc agagtactta agtaattcga ttgagattcg gtaatggctt tgtgtagacg
      181 ttgagataaa accaccgctt tttctttgtt gccattggcg gcgtagatat tcatgagttt
      241 caaatactca tcactataat gggattcacg actgctatct acagcaatag ctttatcaaa
      301 catattcagc gccttatcgg cataatgaat ggcgtttttt atatcgcctt ttgtttgata
      361 accctccgca actgtaagta acatggtgac ttgctgatag ggtataacaa cattatctcc
      421 atatacagaa ttataaatac ttattgcctt atcaggaacg atctgatatg cgactaacac
      481 agtcggtaaa taataattca gggcattctc atcgaggctg agaagctgca tagccttttc
      541 gggttgtttg ctttttatcc agctattgat atagctctca aaggatgtaa gagtagccgg
      601 tttatttaac ccaatttttg ataaaaaagt taaggctgtc tctggtgctt cttcttcagc
      661 gactctggag gcagttaaat ccagataata tttagcgttt ttcgattctg tctcaagttt
      721 gctcagtaaa ttgtaagcac gttcagggta gccagatact aataattcat attgcaaatt
      781 aaaccattca gcctgcgatc tgaaggtggt attttcttcc agaggtaaag cagagtttag
      841 ttttttctcg acgacagaat aaacatccgg ctgagtgggt tcgctggata aaacctgatg
      901 agaataaaaa acagataaaa tcattaat
//
LOCUS       DAZXUK010000556          925 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZXUK010000556 DAZXUK010000000
VERSION     DAZXUK010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.565"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..838
                     /gene="yeeO"
                     /locus_tag="ACH3L8_004509"
                     /pseudo
     CDS             1..838
                     /gene="yeeO"
                     /locus_tag="ACH3L8_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
BASE COUNT          187 a          184 c          267 g          287 t
ORIGIN      
        1 tttacggcct tttctcctgg tcggggctgg gttgggttta accatttctc gttatattgg
       61 cgcagttgca attttgtggg tgctggcgat tggttttaat cctgcgctaa ggatttcgtt
      121 aaagagctat tttaaaccgc tgaattttag cattatctgg gaagtcatgg ggattggtat
      181 tccagcgagt gtcgaatcag tgttatttac cagtggtcgg ttattaaccc aaatgttcgt
      241 tgccgggatg gggaccagcg ttattgccgg aaattttatc gcgttctcaa ttgcggctct
      301 tatcaactta cccggaagtg cgctcggttc tgcttctacg atcattacag gccgaaggtt
      361 gggggtaggg cagatagcgc aagcagagat tcagttgcgg catgtgttct ggctgtccac
      421 tcttggatta acggccatcg cctggctaac ggctcccttt gccggggtta tggcatcgtt
      481 ttacacccag gatccacagg ttaaacacgt cgttgtgatt ctgatttagc taaatgcttt
      541 atttatgcct atttggtccg cctcatgggt gctacccgct ggatttaagg gtgctcgtga
      601 tgcccgttac gccatgtggg tttctatgtt gagcatgtgg ggttgccggg ttgtagtcgg
      661 ttatgtgcag ggaatcatgc ttggctgggg tgtggttggt gtctggatgg gaatgtttgc
      721 cgactgggct gtgcgggccg tgctgtttta ctggcgaatg gttactggac gttggctgtg
      781 gaaataccct cgatccgagc cgcaaaagtg tgaaaaaaag ccagttgtgt cggaataaac
      841 gacaaaatgc agattatttc agcaaacgat ttcaaattta aaaaacaggc tttgacattg
      901 tgggtgggca tcgctaatat tcgcc
//
LOCUS       DAZXUK010000557          924 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZXUK010000557 DAZXUK010000000
VERSION     DAZXUK010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.566"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..829)
                     /locus_tag="ACH3L8_004510"
     CDS             complement(<1..829)
                     /locus_tag="ACH3L8_004510"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFT4087288.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     "
BASE COUNT          276 a          205 c          164 g          279 t
ORIGIN      
        1 atcttggccc cttatgaaag gccagcaacg atgaaaaaaa cggggttgta taataaaaaa
       61 gtcgtctttt cagccatgcg caactttccc gttctttggt gcttattctt cctatccgtg
      121 tagttccttt agcaggagca ttcattcttc tttctgttgt atataaataa tagaatccaa
      181 gttcttcagc gacctgaata taatccttgt tataataacc ttccggccaa cacaaatgtt
      241 tactgcattt tcctgtcatt tctttcaaat attccctccc gctcaacaag tcctgccgaa
      301 gatgtttaca ctgttcttct cgtgaactga actttttatc ccagcgtgta tgagtatgcg
      361 tatgaacatg aaattcgacc agaccagatt gcaacatttc gtttacttca gaccagcgaa
      421 gcataacatt atcagcattc cctgtcgcta tttgatgttc gcaatcgcga tgagaatatt
      481 cctttccagg agagtgacga accggaccat tcccaataaa accggtaatt aaaaaaatat
      541 gtgctttgag attaaactct tttaataaag gatatacctg aaaccagttg tcaaggtatc
      601 catcatcaaa tgtcaacata acgcttttcc gggggagttt tccgccccga taaaaaaact
      661 ccagttcatc tgatgacagt gttttccagt tgttttcagc caaccatttt atttgcttcc
      721 ggaatgtcac gggagaaagt gtaaccagtc cggggcaatg actgacatga tgatacatta
      781 ataccggaac atgattggct ttgaacaaaa tgcccccttc gtttaacatt taaagtttct
      841 ggcacctagc aagcagagac aaaaggaaaa cccaataaag ctggtgcatt ttatttccag
      901 atactggatg aataacatag caaa
//
LOCUS       DAZXUK010000558          923 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZXUK010000558 DAZXUK010000000
VERSION     DAZXUK010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.567"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..200
                     /locus_tag="ACH3L8_004511"
                     /pseudo
     CDS             1..200
                     /locus_tag="ACH3L8_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            300..>923
                     /locus_tag="ACH3L8_004512"
     CDS             300..>923
                     /locus_tag="ACH3L8_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFT4087289.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          293 a          217 c          205 g          208 t
ORIGIN      
        1 attggctacg taattaaaac agaaaataaa gaaaccccat tcactgtagt tgatatcgac
       61 ggtctatcag gaaactttaa aactcttgtt gaaggtgcca caaaaatgtg cctagtttac
      121 atcggaaata atctgcccgc agaaaaaaag ccgaatttct ggcaaatctg attgcaatga
      181 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacag tgtgcaggcc tgagtgaaga
      241 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
      301 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg
      361 tcgtcctgtg ggcaagcgac gattttgaat cgaccagcac cactctggac tatctgctcg
      421 ttaagtcagg taaaaaactg agcaactatt ttaaagctgt tgccacaaat tttcctgtcg
      481 ttaatgacct tccccctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca
      541 gcaaagactc catgacatgg gaactaaaac cgggagcagc gccagacgac gttcaccacc
      601 aggataatgc tcaagaaacc aaagaactgg cgggaggcca ggaagaaaac gcgcaggcag
      661 acgcccacga ggattgccag gattgcgaag tctctgtagc cactttgcgg ttcacacagc
      721 gtcttctgca catttttacg tatgctgccg gggatctgaa atacctgcat catgccaccc
      781 gtgaacaacg caaacacatt actgctcttg agatggatca ggaaaacagc tatgtccaga
      841 atctgctgtt ggccatacgc ggcatggcag aaccgacaac tctggataat gccgccctgc
      901 tccgcctgac tgatgcaatt aag
//
LOCUS       DAZXUK010000559          923 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZXUK010000559 DAZXUK010000000
VERSION     DAZXUK010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.568"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            186..824
                     /gene="rutR"
                     /locus_tag="ACH3L8_004513"
     CDS             186..824
                     /gene="rutR"
                     /locus_tag="ACH3L8_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFT4087290.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
BASE COUNT          238 a          200 c          258 g          227 t
ORIGIN      
        1 tttttaaaaa gtgaatatta ttaatgtgtt agcattgtgt tatctaaaat atctggtaaa
       61 aagtggacta aacggtcaaa acagttgcac ataaaacatg catccgtgcg cgatgagagt
      121 gcagaaggtc gaggccgggc gggggttttg ctatcctgtt accaatctac aagaggggag
      181 agcgcatgac gcaaggcgca gtgaaaacaa cgggtaaacg ttcgcgcgca gtaagcgcga
      241 agaaaaaagc gattcttagc gcagcactgg acactttttc acaattcggt tttcacggca
      301 caaggctgga gcagatcgca gagttggcgg gtgtttcaaa aaccaatctg ctgtattact
      361 tcccgtcaaa agaggcgctg tatattgccg tgctgcggca gattctcgat atctggctgg
      421 caccgttaaa agcgtttcgt gaagatttcg ccccgctggc ggcgataaaa gagtacatcc
      481 gtctgaagct ggaagtctca cgcgattatc cgcaggcttc gcgcctgttc tgtatggaga
      541 tgctggcagg cgcgccgttg ttaatggatg aactgacggg cgatttgaag gcattaattg
      601 atgagaaatc ggcgctgatt gccggttggg tcaaaagcgg caaactcgcg ccgattgatc
      661 cgcagcattt gatttttatg atttgggctt ccactcaaca ttacgccgat ttcgcccctc
      721 aggtggaggc ggtgacaggc gcgacgttgc gcgatgaggt atttttcaat caaacggttg
      781 aaaacgtgca gcggattatt attgagggga ttcgaccacg ttaaagatga cggaggaggt
      841 tgtaacatcc tccggctacc tgtttaaccg atcgtcatta aactggcgtt accgccagcg
      901 gcagcggtat tcacactcag cga
//
LOCUS       DAZXUK010000560          918 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZXUK010000560 DAZXUK010000000
VERSION     DAZXUK010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.569"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            144..746
                     /gene="fimB"
                     /locus_tag="ACH3L8_004514"
                     /pseudo
     CDS             144..746
                     /gene="fimB"
                     /locus_tag="ACH3L8_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
BASE COUNT          280 a          162 c          188 g          288 t
ORIGIN      
        1 atttaagttc gctcagaaga actggtccac ttacgtaagt tattaagcaa acgttcgctt
       61 ttataaacat aatcaggata aaaatgttgg attattgcta acccagcaca gctagtgcgc
      121 gtctgtaatt ataagggaaa acgatgagga ataaggctga taacaaaaaa aggaacttcc
      181 ttacccatag tgaaatcgag tcactcctta aagcagcaaa taccgggcct catgcaacac
      241 gtaattattg tctgattttg ctttgtttta ttcatggttt ccgggcgagt gaaatttgtc
      301 gattgaggat ttcggatatt gatcttaaag caaagtgtat atatatccat cgattaaaaa
      361 aaggcttttc aacaacgcac ccgctattga acaaagaagt tcaggcttta aaaaactggt
      421 tgagtatccg tacttcttat ccgcatgctg agagcgagtg ggtattttta tcacgtaagg
      481 ggaatccgct ttctcggcaa cagttttacc atattatctc gacttctggt ggtaatgccg
      541 ggttgtcact ggagattcat ccgcacatgt tacgctattc gtgtggtttt gctttggcga
      601 atatgggaat agatacgcga cttatctagg attatcttgg gcatcgcaat attcgtcata
      661 ctgtctggta taccgccagc aatgcagggc gtttttacgg catctgggat agagccagag
      721 gacgacagcg tcatgctgtt ttatagagat agttgagata ccagggatgg tgttaaaaac
      781 tacattattc ttacgatata tcaaaaatga ttaaccattt attaattgat gttattgtaa
      841 gtgtgattct taattatcat aataaacatt aagttaacca tacccataca aaatctaatg
      901 atttatgttc ttcaaaat
//
LOCUS       DAZXUK010000561          916 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZXUK010000561 DAZXUK010000000
VERSION     DAZXUK010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.570"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..122
                     /locus_tag="ACH3L8_004515"
     CDS             <1..122
                     /locus_tag="ACH3L8_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFT4087291.1"
                     /translation="PLKCELRGGQMRFTGLKRGYRLAEQVMMHEPLARIRWCG"
     gene            309..546
                     /locus_tag="ACH3L8_004516"
                     /pseudo
     CDS             309..546
                     /locus_tag="ACH3L8_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            660..916
                     /locus_tag="ACH3L8_004517"
                     /pseudo
     CDS             660..916
                     /locus_tag="ACH3L8_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          283 a          175 c          248 g          210 t
ORIGIN      
        1 acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt tacggggctg aagaggggtt
       61 accgtctggc agagcaggtt atgatgcatg agccgctggc tcgaatacgg tggtgcggct
      121 gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa cggagcaaaa aaccatcaga
      181 gatacattga atcaatcaat gtaagcaata ttaagtgtct ataccgatta cttgatgatc
      241 aggttgtgtt gaataaacgc gcattcttcg gtgctttttt aatcttttgg ggatttaaat
      301 tcctatcgat gaactgtcga tatgaaaaag cgagcatcat actgacgtca aacaaagggg
      361 tcgcagactg gggagaaatg ttcggagatc acgtgctggc aacagcgatc ctagatcggc
      421 tgctacacgc tgaatatcaa aggagagagt taccggttaa aagagaaacg taaagctgga
      481 gtgctgacca aaaacacaac gccaatcagt gatgatgaaa tggtggaaaa cggacagcat
      541 cagtaacgaa agtatcttag caggcatgaa aatcgtccgc atcccctaaa cggtcaaaat
      601 gcttctgccg tgaaggaggg agaatgccat ctcatcactt atatctggcg aatgaaacac
      661 agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa tggaatggca gaaaacttcg
      721 tcaaaacgat gaaatatgat taaatcagtg ttatgttcga acctgacgtg ttaacagcag
      781 taaagtagtc tcgcgaaggt gttcaaacat tataatgaat ggcatcctca tagtgcgcag
      841 ggttatcgct tgctgcggaa atatctgaag cgacgtacca gtaatgggga aaatgatagc
      901 tggtttctga aaatat
//
LOCUS       DAZXUK010000562          916 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZXUK010000562 DAZXUK010000000
VERSION     DAZXUK010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.571"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..382
                     /gene="ipgA"
                     /locus_tag="ACH3L8_004518"
     CDS             <1..382
                     /gene="ipgA"
                     /locus_tag="ACH3L8_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HFT4087292.1"
                     /translation="KLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYIFLT
                     GLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLSPDE
                     LIESLYEFLFCIKLTIANITSEVN"
     gene            398..>916
                     /gene="ipgB1"
                     /locus_tag="ACH3L8_004519"
     CDS             398..>916
                     /gene="ipgB1"
                     /locus_tag="ACH3L8_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HFT4087293.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLV"
BASE COUNT          340 a          163 c          138 g          275 t
ORIGIN      
        1 caaactatat gataaacttt atgaaataac aggtgcaaag cttgatttta acgataaaaa
       61 tcaagccttt atattgctcg aagaacaaat acctgtttgt ataacagata atgatgaata
      121 catctttttg accggacttc ttaatgaaca tgaacttttt acagaaaata ttattaatcc
      181 agaacacata ctcatcctta actactcttt gagtagagat tatggttctt ctatctgtct
      241 tctccctgat actcatcaat gtgttttgac caagaaacac tacaaaaaat atctttcacc
      301 tgatgaactc attgaatctt tgtatgaatt tttattttgt attaaactaa ccattgcaaa
      361 catcacttca gaagtgaact aacatatagg gggtatcatg caaattctaa acaaaatact
      421 tccacaggtt gaattcgcga ttcctcgccc atcatttgat tcgttaagtc gcaataagct
      481 tgtaaagaaa atattatctg tttttaactt aaaacaacgc tttcctcaga agaattttgg
      541 atgcccggtg aatataaata aaatcaggga cagcgtcata gacaaaataa aagatagtaa
      601 ttcggggaat caactatttt gttggatgag ccaagagcga accacttatg tctcttcaat
      661 gataaacaga agtatagatg aaatggccat acacaatgga gttgttttga cctcagataa
      721 caaaaaaaac atatttgcag ctattgaaaa aaaattcccg gatataaaac ttgatgaaaa
      781 atcagcacaa acatctatca gtcatacagc actaaacgag attgcctcat ctggcctcag
      841 agcaaagatt ttaaaacgct atagcagtga catggatttg tttaacacac aaatgaagga
      901 tcttacaaat ctagta
//
LOCUS       DAZXUK010000563          915 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZXUK010000563 DAZXUK010000000
VERSION     DAZXUK010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.572"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            30..302
                     /locus_tag="ACH3L8_004520"
     CDS             30..302
                     /locus_tag="ACH3L8_004520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087294.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
     gene            complement(523..761)
                     /locus_tag="ACH3L8_004521"
                     /pseudo
     CDS             complement(523..761)
                     /locus_tag="ACH3L8_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          244 a          176 c          211 g          284 t
ORIGIN      
        1 atccgtttta ttgaatcgga gaacaaactg tgagctatca gcctggtggt ctggttttgc
       61 ctgatttaag ccgccgtggg tatttgtcca agaatgcttc aagctccaat cgttttggcc
      121 tggtgaatgg agatgggtta agcaaagact ctcgttgctg gtgtactttt tcgcgctggc
      181 gctcaagcat ggataatgcg attgcctgta gttcttcgtt agtcattgtc tgcacctgga
      241 aagcaattta ccggagtacc tcacactacg gcaaaaatgt tttattgttg caacttgcgt
      301 aattattcag gtcagtttgg aacgcactgt ataaacataa ataacctgat actgttgtgt
      361 gttttcaaag aaaacacagg tatttttcac ataaaccttc cccttttcat attcgaccac
      421 ttaatcaaaa aacggtttta tacttgttgc ccccactggg gttaaatgtg agacatgtcg
      481 cactttgtgc catgtgtcct cgctgatttt aggcgcggtt cattcattct ttctgttggc
      541 ataaagtatg gtatcggttg ggcgaatgaa agggactcgg gtcatggtca gacaatacct
      601 gaggcgttaa ttcgttgaaa gagattgtga atgggggaat aactgagtct taactgctgt
      661 gcgatagccc ggacagatag ttgctcgtcg aatctgagac gtaatgattc tggcaaataa
      721 gtgcgaaagc cggtgatatc tagtgttgtt tgagagttca tcgttcgcgt ccattaatac
      781 agatggacgc gattgttgcc agacaggata attttcacaa gacatcgcag atagagtact
      841 tacgataatt ttttatttta tacaacagat gcccacgaaa agaattattt tcaatagaca
      901 cctgcattac acaaa
//
LOCUS       DAZXUK010000564          898 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZXUK010000564 DAZXUK010000000
VERSION     DAZXUK010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.573"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            56..>898
                     /locus_tag="ACH3L8_004522"
     CDS             56..>898
                     /locus_tag="ACH3L8_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFT4087295.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTV"
BASE COUNT          302 a          170 c          178 g          248 t
ORIGIN      
        1 cgttgtaatt aaatgctatc ccataaccac aactcagaaa tatcggagtt cacgtatgaa
       61 taaaatttat tcactgaaat atagtcatat tacaggtgga ttagttgctg tttctgaact
      121 gacccggaaa gttagtgtcg gtacatcaag aaagaaagtt atcctcggta ttattttatc
      181 ctcaatatat ggaagttatg gcgaaacagc atttgcagca atgctggata taaataatat
      241 atggacccgc gattatcttg accttgctca aaacagagga gagttcagac cgggtgcaac
      301 aaatgttcaa ttaatgatga aagatggaaa gatatttcat tttccagaac tacctgtacc
      361 tgatttttct gctgtttcca acaaaggtgc aacaacatca attggaggtg cgtacagtgt
      421 tactgcgact cataacggta cacagcatca tgcaataaca acacagtcat gggatcagac
      481 agcatataaa gcaagtaaca gagtatcatc tggcgacttt tcggttcatc gtctgaataa
      541 attcgtcgtg gaaacaacag gggttacgga gagtgccgac ttctcacttt ctcccgaaga
      601 tgcgatgaaa agatatggcg taaactacaa cggtaaggaa caaataattg gcttcagagc
      661 aggtgccgga acaacctcaa cgatattaaa cggcaaacaa tatctgtttg gacaaaacta
      721 taatcccgac ttgttaagcg caagtctttt taatctggac tggaaaaaca agagttacat
      781 ttataccaac agaacccctt ttaaaaactc accaattttt ggcgatagtg gttctggttc
      841 ttatctatat gataaagaac aacaaaaatg ggttttccat ggtgttacca gtacagtt
//
LOCUS       DAZXUK010000565          893 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZXUK010000565 DAZXUK010000000
VERSION     DAZXUK010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.574"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..143
                     /locus_tag="ACH3L8_004523"
     CDS             <1..143
                     /locus_tag="ACH3L8_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFT4087296.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            127..486
                     /locus_tag="ACH3L8_004524"
     CDS             127..486
                     /locus_tag="ACH3L8_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087297.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLEN
                     VNITHWAVMPKAPEAGA"
     gene            486..791
                     /gene="yfdT"
                     /locus_tag="ACH3L8_004525"
     CDS             486..791
                     /gene="yfdT"
                     /locus_tag="ACH3L8_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFT4087298.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            707..>893
                     /locus_tag="ACH3L8_004526"
     CDS             707..>893
                     /locus_tag="ACH3L8_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4087299.1"
                     /translation="MGYDSRLDRLAATSWYPFLNNVTARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQAT"
BASE COUNT          280 a          194 c          227 g          192 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggagaaggct ggctaattgg ctggcgttct
      361 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
      421 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
      481 gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag cgtatcagca
      541 gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc ctatctctgg
      601 aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt tttggtgact
      661 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
      721 tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg tcacagcaag
      781 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaagat
      841 atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa cac
//
LOCUS       DAZXUK010000566          887 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZXUK010000566 DAZXUK010000000
VERSION     DAZXUK010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.575"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            154..>887
                     /locus_tag="ACH3L8_004527"
     CDS             154..>887
                     /locus_tag="ACH3L8_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFT4087300.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESR"
BASE COUNT          283 a          157 c          205 g          242 t
ORIGIN      
        1 tagaagaaat aacaaaatta agaaaagcca tatttacgat atgggcaaca taaatataat
       61 ctaaaaaaga gtaaggcgtt gtaggttggt tccgcgttca ttaccttgtt agtgaacgcg
      121 gatttatcag ttgtcgaaat ggtttttata ataatgaaaa attttaatgt actaaccaaa
      181 gtcgtatttt gcttgtcgtt tttatcaagc gcatttccca cctatgctgc ggatgatgtc
      241 aaagtgactc aaaacacttc agtttttaaa ctgaagctta gtgcaacgcg attaatctat
      301 gaacaagggt cggatggcgc agcgataact gtgacaaatc cgcaggattg gccgatgctt
      361 gtgcagagta aagtctttga agaagataaa gtaactgcag cgccctttat tgtcactccg
      421 ccactgttca ggcttaatcc aggccagcaa aataaaatgt gggttgtgca gactgcgcca
      481 gctttacaag acagggaaaa attacactgg ctatgcgcca ccggcattac gcctaaaaag
      541 agtgatgttt gggccgagaa agataaagaa aaaattgctg aaaatgtgaa cctgaacatc
      601 caggttaacg tacatacctg cataaaactt attaccaggc catctggtat tgaaggcaag
      661 atggttaatg cgagtgagaa gttatcgtgg aaaaagcagg gtgataaaat tgtcgcggtc
      721 aatcagtcgc cgttctacat caacctttct tctttacagg ttggcggtag caagattgag
      781 aaactagatt atattgcgcc gttttcgcaa catgagtttg ttatgccaaa aactagcggc
      841 caggatatta aatggtctgt gattactgat gaaggtggtg agagtcg
//
LOCUS       DAZXUK010000567          881 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZXUK010000567 DAZXUK010000000
VERSION     DAZXUK010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.576"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>881
                     /locus_tag="ACH3L8_004528"
     CDS             <1..>881
                     /locus_tag="ACH3L8_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFT4087301.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVK"
BASE COUNT          275 a          169 c          226 g          211 t
ORIGIN      
        1 gtctataacc acgaaccgtt atgcatcagg ttatgccaca ctgactgaag cagtaagtgc
       61 acaggatgaa cggaacagaa aaagagataa gaatagtcac gatggctcta cgatatcctt
      121 atcccagccc cttggtaata tcgggaattt gaacttcaat accacgaggt ataattcttc
      181 ccggggcaca ggaaacacgc ggtctaccag cctgagttat agtacggtat ggagaggcat
      241 aacattctcc attaactggg caaaaaacga cttgctgaca tcgcataaat ggaaagtaga
      301 tcggaaatta agcgtgggta tttcagtgcc attatcgctg ggtgacgaaa atcaaattta
      361 tgcgtcttca cagatgagtc gttcgggaga gcagggtaat aactatcagg tttcattatc
      421 aggtcagaat agtggtggtg tatggtggga tgtagcgaca aatataacta acgctcatca
      481 gtcacaaccg aagagcacga tgaacattgt gcaagttggc aaaaatggat catatggtca
      541 gtttagtagc cattacagct ccagcgaaaa catgaaacag ctaggtgcaa atttaagcgg
      601 tgggatactt attacaaggg atggacttac atttggtcaa aatgtggatg ggacattagc
      661 ccttatagag gcacccggag caacgggggt taacgttaat ggctggccgg gactgagtac
      721 tgattttcgt gggtatgcaa tactgccagt gcagccatac cgaagagacg atgttattct
      781 ggatgaaaaa acgataggta agaattacga cttaccacaa actagtcaat tggttgtacc
      841 gacagctgga gccgttgtgc ctgcgacatt ggcagtgaag a
//
LOCUS       DAZXUK010000568          880 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZXUK010000568 DAZXUK010000000
VERSION     DAZXUK010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.577"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..800)
                     /locus_tag="ACH3L8_004529"
     CDS             complement(<1..800)
                     /locus_tag="ACH3L8_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFT4087302.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMPYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFEL"
BASE COUNT          253 a          160 c          180 g          287 t
ORIGIN      
        1 tttaattcaa acagttctgg aagagatgac ggttgcagag tcatgcgttt gagtggcgca
       61 tcttcatcac cggggataac gttatatccc atttttgaat ggatctccgc ggtaccagga
      121 taggcttcta taccaatgat tgggtcattt cccatctcaa tattagcatt aagtaatgcg
      181 gcaagggcga atccggaaga atttgctacc cctgagacag ttattatctg atcaatatag
      241 atatcaattc ttggctgctc attggtccat ctttttgcct gagaagctgg aagggttgaa
      301 atatatccta tcgtggttcc ggcataacca ggaacattgc aaccagggcc taatgttatt
      361 tcatgaactg tatagtcctc aacaggatcg aaagggctct tcataaataa atttagccgt
      421 aaaccttctg ctaactctgt agcagcatgc gcctttttac atataccacc atatatttcg
      481 tctaaagctg aggtgatttt caagtttcga tagggcatac gtcctgttgt tgtaatacca
      541 ttgaattctc tgcatgtatc tgtcacataa ttaaatggcc aggactgttt gccaatatta
      601 agttttctaa tttcgacaac gctttttcca tcgtaattat ggttattgcc acgagcaaga
      661 ggcagaagat agatattttc cgtatccagg cgtgcatatt catatcgacg tgttaactca
      721 ctgtcgactt ttggatgatg aaggaagtga ttatttgtat tgaattggtt attagcggca
      781 ttgcttaatg gcatagacat aatagttacc ttttatgagt atttaactga tgtttagaaa
      841 aatagataaa tttttctgta gtaaaaagag aagtaaacaa
//
LOCUS       DAZXUK010000569          876 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZXUK010000569 DAZXUK010000000
VERSION     DAZXUK010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.578"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            21..698
                     /gene="tnpA"
                     /locus_tag="ACH3L8_004530"
     CDS             21..698
                     /gene="tnpA"
                     /locus_tag="ACH3L8_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFT4087303.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPSGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            698..>876
                     /gene="tnpB"
                     /locus_tag="ACH3L8_004531"
     CDS             698..>876
                     /gene="tnpB"
                     /locus_tag="ACH3L8_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFT4087304.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWA"
BASE COUNT          209 a          239 c          224 g          204 t
ORIGIN      
        1 cctccggctc tacaaaaata atgtccatca tttttaatgg acactatcgt atgaaacacc
       61 ggacctggat cactgaagct ttacgtcttc actttgaaga acatttaccc cgggttgtgg
      121 ccgggcgtcg cctgggtgta ccaaaatcaa cagtttgtag tatgtttgtg cgctttcgga
      181 gagctggcct ttcgtggcct ttgccctcag gcatgtcgga gcaggaactt gatgcctgcc
      241 tttacggaca attttccacg gtaccagtcg tacgtcctga aagcaccgtt atatccgaag
      301 cccccgtggt aaaaaaacgt ccccggcggc ccaacttccc ttatgagttt aaaatcgcct
      361 tagtggagca gtcactgcag cccggagcct gtgtggcgca gatcgcccgg gaaaacggaa
      421 tcaacgataa cctgctcttc aactggcgcc atcaataccg gaaaggtggc ctgctgcctt
      481 ccggaaaaaa tatgccggca ctgcttcccg tgacgttaac gccggagccg gataataaaa
      541 tcccggctcc ggcacaggaa ccagagcaga taaatacacc gtccgacagt ctgtgttgtg
      601 agctggttct gccggccgga actctcaggc ttaaaggtaa actgacgccg gcgttattac
      661 agacacttat ccgcgaaata aaagggagca gccactgatg atatctctcc ctgcaggttc
      721 gcgtatctgg ctggttgccg gtatcaccga tatgcgaaat ggctttaacg gcctggcctc
      781 aaaagttcag aacgtcctga aggatgaccc gttctccgga cacctgttca tcttccgcgg
      841 acgccggggt gaccagataa aagtgttgtg ggctga
//
LOCUS       DAZXUK010000570          870 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZXUK010000570 DAZXUK010000000
VERSION     DAZXUK010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.579"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>870
                     /locus_tag="ACH3L8_004532"
     CDS             <1..>870
                     /locus_tag="ACH3L8_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFT4087305.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          263 a          153 c          209 g          245 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga
//
LOCUS       DAZXUK010000571          867 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZXUK010000571 DAZXUK010000000
VERSION     DAZXUK010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.580"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..608)
                     /locus_tag="ACH3L8_004533"
     CDS             complement(<1..608)
                     /locus_tag="ACH3L8_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFT4087306.1"
                     /translation="MTGEKESKNAERIFMPPEIMRFKERLEEAMNGESSRAFAAKCGL
                     SDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDR
                     STLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQ
                     TAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            778..>867
                     /locus_tag="ACH3L8_004534"
     CDS             778..>867
                     /locus_tag="ACH3L8_004534"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4087307.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSRE"
BASE COUNT          233 a          180 c          174 g          280 t
ORIGIN      
        1 tatacggctt tttttacatc ttgtggttct tgtgaaggag ctacaagacc ttcctgttca
       61 taaataccat actttgacaa aatcttttta cgagtatctg ttggtaatga ttcaagcatt
      121 ttggctgttt gtaaggtttg ctgcgaaata ccttgcaaat cagcctggat ggaatcatcg
      181 gaagaaaaaa tctcaggcat aagagcttca aatcctttct ttctgaacaa ctcaatcgca
      241 gaatgcagtt ccttgtcctc taacaattcg acaattgcga tcagtttagt tttcaatgtg
      301 ctgcggtcag gttgagaatt tttattctct tgattatttt tatgctcaac cattggcatg
      361 ctctgacttt ggataaacca ctctattgga cgccctgttg cgtaggctat ttgtgctaat
      421 cgatctaaag acgggtatgt cttgccagac aggtagtttc tgataacccc atcagaaagt
      481 ccgcacttag ctgcaaacgc ccggcttgat tctccattca ttgcctcttc aaggcgttcc
      541 ttgaaacgca ttatttccgg gggcataaaa atgcgctctg catttttgct ttctttttca
      601 cctgtcatgc ccaatctctc tgttatataa agaattttac agaattcttt gatttgcact
      661 ggttaaaata aaaatgcgca tagcttttga attgagcatt tttctgcgct atcgtttttc
      721 ccgatagcgg tagccggtat cagttaccga aaacgttaag agagtaagga tcgcagaatg
      781 tcagcaaaaa accaagactg gcacgcagag cagataaagg ctgctgttcg tatgaagggg
      841 aagacactat ctcaattatc cagagag
//
LOCUS       DAZXUK010000572          863 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZXUK010000572 DAZXUK010000000
VERSION     DAZXUK010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.581"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..549)
                     /locus_tag="ACH3L8_004535"
     CDS             complement(<1..549)
                     /locus_tag="ACH3L8_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFT4087308.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKC"
BASE COUNT          246 a          201 c          178 g          238 t
ORIGIN      
        1 acatttcgag gattttttac tttcggtatt tacggtggcc atcatccacg ggcatgccgt
       61 agtacaggcg ctacagccaa tacaacgttt atgatcgacg gtaatacagc cttctttctg
      121 ctgccaggta atcgcaccaa tcgggcagac gttcatgcat tgcggctctt tgcattgacg
      181 gcaggtgtcc gcggtatagt tgagatcgcc atacaggccg ccgccagagc caaccccgtt
      241 gtcgccaaag aaataattgc gatggatttt gatacgggag aagaatgtcc ctactgagcc
      301 atcgttgaag ttggtacaag agatttcaca gcggtggcaa cccgtgcatc gcgctcgctg
      361 ggtcaccagc acccctttgg gtgtatttat caaccctact gtgccgctat caatatcttc
      421 ctgtttgcaa cccaaaagtg acagtaacgc aggggcaatt gttagaccag caaggccttt
      481 tcctgatata cgcaagaatt ctaatcgcgt taagccaata tctaatagtg gacgatcaac
      541 cgggttcatt tatttcgcat cctcttcgca aacacagtgc tgacaattca gcagcctgat
      601 attaagcatt caactaatac gctgctgact ctgtccccaa caccagatat tttccctaac
      661 ccgatagtgt atgagaaaag atttaacgcc gataaactaa tcctttatgg ttaggagaaa
      721 aaataatgtg attattctga gcccttaaca ttgatcgtta tcaattaaaa ttacaaagca
      781 agaaattata agcgaactga tatttattat catttgaaat aaatttaact taagcgacaa
      841 tcatttgtga cgttcattgc aga
//
LOCUS       DAZXUK010000573          854 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZXUK010000573 DAZXUK010000000
VERSION     DAZXUK010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.582"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            58..>854
                     /locus_tag="ACH3L8_004536"
     CDS             58..>854
                     /locus_tag="ACH3L8_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFT4087309.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLT"
BASE COUNT          292 a          205 c          120 g          237 t
ORIGIN      
        1 aaagcagata aaaaagaatc caacacctct caacataaag aatacaccct gatatttatg
       61 ctcccgacaa ataacaatca cagattaatt tcaaattcgt tctccactta ttcaatcgac
      121 actagccgcg catatgaaaa ttatctaacc cattggactg aatggaaaaa taaccgcata
      181 caagaagaac aacgagacat cgcttttcag cgactagtat catgtctaca aaaccaagag
      241 acgaacctgg acttgtctga attaggcctg acaacattac ctgaaatccc cccgggaatt
      301 aaatcaatta atataagtaa aaataattta agcttaatct ccccattgcc tgcgtccctt
      361 acacaactta atgtcagcta taacagactt attgaactgc ctgctttgcc tcaaggactt
      421 aaattattga atgcgtccca caatcaacta atcacactac ccacactccc catatctttg
      481 aaggagcttc atgtctcaaa taatcaatta tgttctcttc ctgttttacc agaactactg
      541 gaaacattag atgtatcatg taatgggctg gcagttttac cacctttacc attttcttta
      601 caagagatta gcgcaatagg gaatcttctt agtgaactcc cccctctacc tcacaacatt
      661 cactccatat gggcaatcga caatatgtta accgatattc catacctgcc ggaaaattta
      721 aggaacggtt attttgacat aaatcagata agtcatatcc cggaaagcat tcttaatctg
      781 aggaatgaat gttcaataga tattagtgat aacccattgt catcccatgc tctgcaatcc
      841 ctgcaaagat taac
//
LOCUS       DAZXUK010000574          854 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZXUK010000574 DAZXUK010000000
VERSION     DAZXUK010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.583"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..470)
                     /locus_tag="ACH3L8_004537"
     CDS             complement(<1..470)
                     /locus_tag="ACH3L8_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4087310.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLT"
     gene            complement(467..>854)
                     /locus_tag="ACH3L8_004538"
     CDS             complement(467..>854)
                     /locus_tag="ACH3L8_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFT4087311.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT          248 a          226 c          179 g          201 t
ORIGIN      
        1 tcagtgagcc agccccccag agttggccct agcgtcggtg acagtgaggc cagcgcactg
       61 actaccgcag cagccaaccc caactcttta ccctggtaat aaataaacgc catcgtgaaa
      121 actaaaggga tcatggatgc gccagcagct ccctgtaaag cacgaaacaa aatcatgatc
      181 tgaatattcc atgcaagtcc acacgcgata ctcattaaag taaatattcc cgcggataga
      241 gtaaaaagcc aacgagtgga aaatacccgt gacagccacc cagagagcgg aataacaata
      301 atctcagcaa ttaaatagct ggtctgaagc caggccattt cgtctttacc tgcggacaga
      361 ccaccaccta tttcatttag ggatgacgat acaatttgaa tatcaagctg agcaataaat
      421 aatcccatac acatactggc gaaagcaata tgtttacgca tagacatcat ctctcatcaa
      481 cactgacggt gacggacagt cccgggcgaa gaagttgctt taattccttt gcgtcgtcaa
      541 atgcaatccg taccgggaca cgctgaacaa ttttggtgaa attgccagtg gcattttcta
      601 tcggaatcaa gctgaaactg gctcctgtcg ctggggaaag gctttcaatg tgaccgtgaa
      661 acacttcccc tttgagaata tcagcacgga tctcagcctt cattcctggc ttcattcccg
      721 ctatttggtt ttctttataa tttgcatcaa cccacaactc actgactggt actagtgaaa
      781 ctaacgatgt gccgccttcc acccaactgc ctgtatgggc gctgcggttt gcgacaatgc
      841 catcaatcgg cgca
//
LOCUS       DAZXUK010000575          843 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZXUK010000575 DAZXUK010000000
VERSION     DAZXUK010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.584"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..746
                     /locus_tag="ACH3L8_004539"
     CDS             <1..746
                     /locus_tag="ACH3L8_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4087312.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT          243 a          184 c          192 g          224 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgc
//
LOCUS       DAZXUK010000576          841 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZXUK010000576 DAZXUK010000000
VERSION     DAZXUK010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.585"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..210)
                     /locus_tag="ACH3L8_004540"
     CDS             complement(<1..210)
                     /locus_tag="ACH3L8_004540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087313.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDV"
     gene            complement(356..>841)
                     /locus_tag="ACH3L8_004541"
     CDS             complement(356..>841)
                     /locus_tag="ACH3L8_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087314.1"
                     /translation="QFRWLAINNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMY
                     HLPLARSLVEHHAIVVEQYLRFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIAC
                     GLVGCSWEITKTYYPGIIATILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRK
                     Q"
BASE COUNT          320 a          160 c          132 g          229 t
ORIGIN      
        1 aacatcactg ccaactttat taattgcttg ttgcaaatct aatgtggcag gacttatacc
       61 atgtcgagct tgttctgctg tttgaacttg tagatcaatt tcattccata ggtagtgacc
      121 aaatatattt cctccagcag gactaaaagg atcaccagag atccaaaaac tacggacata
      181 ccaataaatg ccaccaagaa ttgttgccaa gctaaaaata agaaatgtct gtatgtttct
      241 ctttttcgct atcaatataa caaatatgat ggctccatat atggcaccaa atagttttac
      301 gccacaagca attccaataa aaaaaccaca taaaaaaatt aattcatttt gtgttttatt
      361 gttttctgag cgaatatata taagaatact ggctgcagca aaacaaagat agcgttaaat
      421 caatatatgc ataaccaaga tgatctttaa gtggattgat agtaaatagt aggattgtag
      481 caataatgcc tggataataa gttttcgtaa tttcccatga acagccaaca agtccacagg
      541 ctattacaaa aaaacagata tttgctaaaa actgagctaa ccttacatct cctaactgta
      601 aacctaaagc cattaaaaga tctgcatttt gcgggaacag aggaaaacgc aaatattgtt
      661 caacaacaat tgcatggtgc tcaactaatg aacgagccaa aggtaagtga tacatagtat
      721 catcccaatc accgggagct cgcagggacg ataacaatgt aaccacaaaa atcaaaacaa
      781 gaaataaaat aacaaacagc gtactgaact caatattatt tattgccagc catctaaatt
      841 g
//
LOCUS       DAZXUK010000577          839 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZXUK010000577 DAZXUK010000000
VERSION     DAZXUK010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.586"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..78)
                     /locus_tag="ACH3L8_004542"
     CDS             complement(<1..78)
                     /locus_tag="ACH3L8_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFT4087315.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQ"
     gene            complement(758..832)
                     /locus_tag="ACH3L8_004543"
     tRNA            complement(758..832)
                     /locus_tag="ACH3L8_004543"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(797..799),aa:Gly,seq:tcc)
BASE COUNT          250 a          209 c          176 g          204 t
ORIGIN      
        1 ctgcaaaaac cagtagccgg cactgccggc ggaggtgccg taggcaatgc ctgttgatat
       61 tttttccatc tgatacatat cccgcccccc cgacagacct gtgctatcgg aaagaaaaaa
      121 ggccatcagc aaaactctga tggcctgaat cacctttacc aatattgtat gaaaaaacac
      181 gcacgactaa ttgacaataa ttttcatttc cattaaaaaa gatcgcgtag cattcttaat
      241 tcatgaggaa ctttacccgc cagcaatctg agtagcgaaa gctgttcatc cccaatgttt
      301 ttgctggcgg gtcctttttt ctacggtcct cctccccgga ggggcatttc attatttttc
      361 taatcgttac tggtgagaag gcggaagaaa aagccagttc ttcgccccta aataacagca
      421 tgattccagt ccatgcaaaa tctgatttcc agctcacaca taccgagcac tgtacaaaaa
      481 accgccagcc aaggcagtta agagtgtgtt cccggggttt gcttagaata ttttgtaagt
      541 tgtccgaaga gatatttaca acaccagaat gatgcattat cagtcccctg ccagaggttc
      601 actacgtaac ctgaccgaca atgcaccatt ctggtgctga aaaaagagca ctggaactgc
      661 aacaaaacaa catcacagaa caggagatgg taaggagtca gacattccca cacaatattg
      721 tgtcagtgca aataacaacc ttcgtctcaa atctcgctgg agcgggcagc gggaatcgaa
      781 cccgcatcat cagcttggaa ggctgaggta atagccatta tacgatgccc gcatatggt
//
LOCUS       DAZXUK010000578          833 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZXUK010000578 DAZXUK010000000
VERSION     DAZXUK010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.587"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..664
                     /locus_tag="ACH3L8_004544"
                     /pseudo
     CDS             1..664
                     /locus_tag="ACH3L8_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          252 a          163 c          177 g          241 t
ORIGIN      
        1 gtttatctga tgggttaatt gatggtctga agatgcttgc tgtggctgtt actgctcaaa
       61 aggctgcaaa tgtagcagga ggcttgttaa accttgcaaa cgctgttact tcattcggtg
      121 acgctgttaa taaagctaaa gctggcaagg ctggttcttc aggtggtgcg ctaaataaag
      181 tcggtagagt tgtcctttat gctgagttgg taaattttgc tcttgataat ttaaaccctg
      241 aagcagctaa gaatccgaac ttacatcaat caggtattgg cacagaaaca aacttcaacc
      301 ctgaatacaa aggtgattca tctaagccaa tagttctatc gctgactggt ttaatggtgc
      361 aactaataaa attgctaact tccaagaacc gatgaataac ttcgtaaata acacacaatc
      421 aactccatca gctatcacag gtgacatggt taatagcaat gctcaattag ctatggctgt
      481 atcagagtta ggaagaaagg tagacgcttt aacagcagaa actaagaagc ctttatcagt
      541 taatatcact ggtgaagcaa ctgttagacc ggatgcacca tctttcttta gcttcaatca
      601 atcagctaat gaccaattca cgcaggatat gctcttaagt tcaagctatc cagaggaaga
      661 atgatttctt atatatccgt gtgctacgtg aaatattctg aacagatata cccactgaaa
      721 aatagtgaac cgccccgggt ttcctggaga gtgttttatc tgtgaactca ggctgccaga
      781 tcatcgtttc cgatggaagc ataataagct ttttctgctt ctgccggagg gat
//
LOCUS       DAZXUK010000579          831 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZXUK010000579 DAZXUK010000000
VERSION     DAZXUK010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.588"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(110..577)
                     /locus_tag="ACH3L8_004545"
     CDS             complement(110..577)
                     /locus_tag="ACH3L8_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFT4087316.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..>831)
                     /locus_tag="ACH3L8_004546"
     CDS             complement(643..>831)
                     /locus_tag="ACH3L8_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HFT4087317.1"
                     /translation="RFAKDERGVTAIEYALIGVAMATLLAFIFGDQNSGFLGAIKDAF
                     DAIAAAIQQVTISGTSNP"
BASE COUNT          294 a          180 c          162 g          195 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac g
//
LOCUS       DAZXUK010000580          831 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZXUK010000580 DAZXUK010000000
VERSION     DAZXUK010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.589"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>831
                     /locus_tag="ACH3L8_004547"
     CDS             <1..>831
                     /locus_tag="ACH3L8_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFT4087318.1"
                     /translation="NNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIRN
                     VNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDELA
                     TFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNPS
                     SHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSVCQKFKWLLPDDDEMCQ
                     WFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRIN
                     "
BASE COUNT          294 a          169 c          145 g          223 t
ORIGIN      
        1 tgaataacga acagatgctt atcaaatgca gagatccccg taaatgtcgg ttttatgccg
       61 aatatctgaa tcacaaatac cctaatgaat tccctcactt tgcgcctgac aacccacaaa
      121 attgtatcag gaatgttaat ttatttttaa aggaaaagta ttgtaatgtt tcacctcccc
      181 cctttggtac ttttggtact actgaagaag aagcactcaa taacgaaatt aatgagcttg
      241 aaattattgc cgggcaggca atactgcctg aagagagttt tctctggttg aaaactgacg
      301 aacttgcgac tttctttacc tggactacta tttatctgtc gaaggaagac catacaaaat
      361 tcccgaaaat aaaccataat tcatcaggaa taaaaaacgg aattgaagtc aggaaaatac
      421 gaacagttac cgatgaaacg ttaaacactt acaaaaaaag taaccttccg gataatccat
      481 catcccataa cgaacgaata aaagtaatca tttcatattt tgactcctgc cattctttaa
      541 ttaataaaaa tgcaaaaagc atatacctcg aggaactcag gcaggcgtgg cttaaaaaga
      601 aaagcgtttg tcaaaagttt aagtggctcc ttcctgacga tgatgaaatg tgtcagtggt
      661 tctggacaag actgaaacaa aaacaggatg aaaaacatcc agtacataac ccagtgaccc
      721 ggtggttcat accatcttca acgaaagaat attatcttgc ctctcttgtc gcctttgctt
      781 tatggaaaga agcgcctgat accattgaac tgttcagaac acgaataaat c
//
LOCUS       DAZXUK010000581          827 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZXUK010000581 DAZXUK010000000
VERSION     DAZXUK010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.590"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..825
                     /locus_tag="ACH3L8_004548"
     CDS             <1..825
                     /locus_tag="ACH3L8_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087319.1"
                     /translation="DAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVL
                     QHYDDMERKGLFGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDA
                     FIEKIPLIDPVYKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDV
                     EKVLDKINILIDKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVE
                     KLGVLVHYVKSPVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
BASE COUNT          279 a          114 c          195 g          239 t
ORIGIN      
        1 gacgcatatc tgaaaaagat tataagctat tttgggcagt tgcagaatga ggctgttcat
       61 gtgaccagct gctacgaaac acttgcggtg tacatttgtc tgataaggaa aaataaaatt
      121 aatgatgtgc tgcagcatta tgatgacatg gaaagaaaag gattgtttgg tgaattacct
      181 tcaggatatg tcaggggggc attatcgttg cttcggacag cactggaggt taaagtaaat
      241 agaaaaaata taaagtatgg ctcgttgttt tattggcttg accatgtaaa agcctatcag
      301 gatgcattta ttgaaaaaat accacttatt gatcctgtat ataaggaggg tgaaatacag
      361 tatgatgcga acaattttac cctgatgcgt gttattaaga tgtataattg tatgcttgaa
      421 aagatcagta cgaaaccata tattgcgccc ccatatatta caggtttgct tgatgacgta
      481 gagaaggtgc tggataagat aaatattctg atagataaag agtatgttta tgatggtaag
      541 acgctggcag aagtcattat ggaaaacaaa gtattatcca gtcgcgaacg taaagaaact
      601 atgatagggc ttttcacagg cagtaagaaa tatactttac tgcaatgtgt tgagaagctt
      661 ggtgtattag ttcactatgt gaaatcaccg gtcgatgaaa ttaaaaatgt gatgatgttg
      721 tatggcgata aggccgaaaa caggaacaga cgcagaatga tatatgatgc gttaaccatt
      781 atttgtgaag atgacatcag gaataaccct cctgagctgt cgtgaat
//
LOCUS       DAZXUK010000582          824 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZXUK010000582 DAZXUK010000000
VERSION     DAZXUK010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.591"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..308
                     /locus_tag="ACH3L8_004549"
                     /pseudo
     CDS             1..308
                     /locus_tag="ACH3L8_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            341..741
                     /locus_tag="ACH3L8_004550"
                     /pseudo
     CDS             341..741
                     /locus_tag="ACH3L8_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          234 a          184 c          235 g          171 t
ORIGIN      
        1 tgctgggcgc tgaaagaact ggcaaaggat atctggagca gaccatggag cgaggaaaga
       61 cgcaacgact ggcagagatg gctgcggcca acagtgacgt ccccatgatg aagaatgtgg
      121 ctaaaacgat agggaaaagg ttgtacggta tcctgaacgc catgcgccat ggcgtatcaa
      181 acggaaatgc ggaagcactg aacagcaaga tcaggctgct gagaataaaa gccaagggct
      241 accgcaaccg ggagcgcttt aagctgggag tgatgttcca ctatgggaaa ctgaatatag
      301 cgttctaagc cttcccacca tgatcgggga agccccagat atgaacaggc acattcagag
      361 ttactttaaa gagcttactg aacgtctgag gtgttagagc catgactgcc gcagcattgc
      421 tggcggaaat gcctgaatcg agcagcctct cgagacgtga aatcagcgca cttgtcggtg
      481 ttgctcaggt aaaccgggac tcgggaactc tgcgcggtcg acgaaccatt ttcggtgatt
      541 gtgctgggga agaacagctc tgtacatggc ggcgcttgag gccccccccg gtttaatctg
      601 gtgataaagg cgttttacat gcgcctactt gcagccggaa acgccaaaaa agtggcactg
      661 gttgcctgta tgcgtaagtt gctgaccatt atgaacgcaa tgctcagaaa aaacgaagaa
      721 tggaatgaat cgtatttatg aaatactgga ttgtgggcca taaatattcc aaaaagatag
      781 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtc
//
LOCUS       DAZXUK010000583          823 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZXUK010000583 DAZXUK010000000
VERSION     DAZXUK010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.592"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            18..>823
                     /locus_tag="ACH3L8_004551"
     CDS             18..>823
                     /locus_tag="ACH3L8_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFT4087320.1"
                     /translation="MANLYDLKKLDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHP"
BASE COUNT          264 a          181 c          148 g          230 t
ORIGIN      
        1 ataaaccgga gtagtgtatg gccaatctct acgacttgaa aaagctcgac ttaaatcttc
       61 ttgtcatatt tgagtgtatt taccagcatt taagcattag caaagctgct gaatcactat
      121 atatcacccc atcagccgtg agccagtcgt tacaacgctt acgtgcgcaa tttaatgacc
      181 ctttattcat tcgctcagga aaaggtatcg cgccgacgac aacagggttg aatctgcatc
      241 accatcttga gaaaaacctc aaaggccttg aacaaacaat taatatagtc aataagtccg
      301 aactaaagaa aaactttatt atctacggcc ctcagctcat ttcttgctct aataatagta
      361 tgctgatccg ctgcctacgg caagatgcct ctgtcgagat tgagtgccat gatatcctca
      421 tgtcagcaga gaatgctgaa gaactgttgg ttcaccgcaa agccgatctg gtgatcaccc
      481 aaatgccagt gatcagccgt tccgtcattt gcatgccatt gcatacaatt cgcaatactc
      541 taatttgtag taataagcat ccgcgaatta ctgacaatag cacttatgaa caaattatgg
      601 cagaagagtt cacccaatta atagccaaat cagcaggagt tgatgatatt caaatggaaa
      661 tagacgagaa gtttatgaat cgcaaaattt catttcgtgg ttcctctttg ctgacaataa
      721 ttaatagcat tgcagttaca gatttactgg gaatagtccc ctatgaactt tataactacc
      781 accgtgactt tcttaattta aaagagataa aactagaaca tcc
//
LOCUS       DAZXUK010000584          822 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZXUK010000584 DAZXUK010000000
VERSION     DAZXUK010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.593"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..124
                     /locus_tag="ACH3L8_004552"
     CDS             <1..124
                     /locus_tag="ACH3L8_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFT4087321.1"
                     /translation="DFRNIYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            481..747
                     /locus_tag="ACH3L8_004553"
                     /pseudo
     CDS             481..747
                     /locus_tag="ACH3L8_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          234 a          200 c          177 g          211 t
ORIGIN      
        1 agattttcgc aatatttatg ctgaatatta cgctgcaacc aaaaaagata aagagattat
       61 cgaccgacga ttacaaatgc gtacaaatga tttttattcg ttattaaaca ggaaatattt
      121 atagacgtat ttgttgatgc tataagccag aggctcttct atgagaatat ggggaccatt
      181 ccctcgcgat attaacgtaa tatcttaatt gtatgcaccg ccagaatgcg ttcagtgcga
      241 tcacggcagc agacaggtaa aaatagcaac aaaccacccg aaaaaccgcc gcgatcgcgt
      301 atgataaatt ttaaccacat gcatacctgt gcaacaaggc gaatcacgca cggatttcca
      361 gccagagaca cctgaaacgt aaccggctca tttaaaccgt ctggtctgtt acctccggtt
      421 ccacaaaaat aatgtccatc attttgaagt ggacactatc gtatggaata ccggacctga
      481 atcactgaag ctttacgcct tcatttcgaa gaacatttac cacgggttgt ggccggatgt
      541 cgcctgggcg taccaaaatc aactgtctgt ggtatgttcg tgcgcttccg caacgctggc
      601 ctttcatggc ctctgcccgc aggtatgtcg gagcaggaac ttgatgccct tctttacgga
      661 agtgcctcca cagtacctgt cgtacttact gaaagcaccg ttatgcccaa actcccggtg
      721 gtaaaaaaac gtccccggcg gcctaatgcc gatcagttaa ggatcagttg accgatccag
      781 tggctgtgta agaatccgga aacgctcact tgtttccgga tt
//
LOCUS       DAZXUK010000585          820 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZXUK010000585 DAZXUK010000000
VERSION     DAZXUK010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.594"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(334..442)
                     /locus_tag="ACH3L8_004554"
                     /pseudo
     CDS             complement(334..442)
                     /locus_tag="ACH3L8_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(501..779)
                     /locus_tag="ACH3L8_004555"
     CDS             complement(501..779)
                     /locus_tag="ACH3L8_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087322.1"
                     /translation="MNIDEASGCFILRQRIDIVNAARAKAFSRLTVLFCTPDRLSGRD
                     VIILNSDAIQRVCDEFMVANSELFALVQEYNRIARTCGMDELRITHLG"
BASE COUNT          251 a          231 c          146 g          192 t
ORIGIN      
        1 accgttgcac tctgcaaata tgcccttatg gactaaaaca acaaagtcca taaggagttt
       61 actcacatct gacaaaatca atataaacag cccctccgga gaggggctgg agagtggcgc
      121 tatgtgccat tccatggtgc cgggtgcctc ccggtgaatt cagtaccagc acctgaatcc
      181 gcgattatcc catataccta ctcgctgatt gcccctccgc acagggggat tcaccatgcc
      241 agtttctttt aacaaactcc ccgcaaacca gacaacagtc aaccgcctga attgtgaagt
      301 atttaaaaat ttctcccgct aactgatacc cggctaacag tctggcgttt tctttttcag
      361 caacgggaaa gcaacaacca ccacacccgc caccagcaca ccgtcagccg gcactgacat
      421 tatccggctg ctgcaatgcc attaacaaaa acagtaagca atcacttttt accgtaacag
      481 gtgataatcc agatatgtat ctaccccaga tgagtaatcc gaagttcatc cataccacag
      541 gtcctggcta ttctgttgta ctcctgaaca agagcaaata attctgaatt agcaaccatg
      601 aactcatcgc aaaccctctg tatagcatca ctattcagaa taataacgtc tcttcccgaa
      661 agacgatcag gagtacagaa caaaactgtc aaacggctga aggcctttgc tcgtgctgca
      721 ttgactatat caatacgctg cctaaggatg aaacaccccg acgcctcatc aatattcact
      781 ctacccacac catatgaatg ataaatactt aatgctgaaa
//
LOCUS       DAZXUK010000586          816 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZXUK010000586 DAZXUK010000000
VERSION     DAZXUK010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.595"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            68..361
                     /gene="mxiI"
                     /locus_tag="ACH3L8_004556"
     CDS             68..361
                     /gene="mxiI"
                     /locus_tag="ACH3L8_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFT4087323.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            367..>816
                     /gene="mxiJ"
                     /locus_tag="ACH3L8_004557"
     CDS             367..>816
                     /gene="mxiJ"
                     /locus_tag="ACH3L8_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFT4087324.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVI"
BASE COUNT          273 a          122 c          169 g          252 t
ORIGIN      
        1 tacagtgaaa gtgattaagg atgttgatgc tgcaattatt caaaacttca gataataggg
       61 agcattcatg aattacattt atccagtcaa tcaggttgat attatcaaag ccagtgattt
      121 tcaatctcaa gagatatcaa gtctggaaga cgtcgtgtcg gctaaatata gtgatattaa
      181 gatggataca gatattcaag tatcacaaat aatggagatg gtaagcaatc cagaatcatt
      241 aaacccagaa tctttggcca agttacagac gacgctctca aattattcaa taggagtatc
      301 attagctggc acgttagcaa gaaaaacagt ttcggctgtt gaaactttat taaagtctta
      361 atttatatga ttaggtataa aggttttatt ttattcttgt tgctgatgtt gattggatgt
      421 gagcaacgtg aagagttaat ttctaattta tctcaaagac aggcaaatga aataatatct
      481 gtgctagaac gccataatat tactgctaga aaagttgatg gaggtaaaca ggggatctcg
      541 gtacaagtcg aaaaggggac atttgcatcg gcagttgatt tgatgcgcat gtacgatttg
      601 ccaaatccgg agagagttga tatctcacaa atgtttccta cagattcatt agtgtcttct
      661 ccaagagctg aaaaggcccg tttatatagt gctattgagc aacggctgga acagtcttta
      721 gtttctattg gtggtgttat ttcggcaaaa atacatgtta gctatgatct tgaagaaaaa
      781 aatatatctt caaaaccgat gcatatatca gtaatc
//
LOCUS       DAZXUK010000587          807 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZXUK010000587 DAZXUK010000000
VERSION     DAZXUK010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.596"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(145..717)
                     /gene="lpfA-O113"
                     /locus_tag="ACH3L8_004558"
     CDS             complement(145..717)
                     /gene="lpfA-O113"
                     /locus_tag="ACH3L8_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFT4087325.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          235 a          189 c          160 g          223 t
ORIGIN      
        1 ctggttggcc taaaaaaacg ctgagtataa tcgcggtaaa taacttacat taaactgttg
       61 ttttaaagag cctgattaaa caactttttc tggacataac gactccctgg ccaaagccaa
      121 ctgaataaaa aggcccttga taaattattt cttatattcg acagtaaatt gtgaatcagc
      181 gttcgccgta ccagccgtta cggtcgcatt ggttgccaca tagcgtgcaa taaacttcat
      241 ttcagccttg ccttcagcat cgatagcggt ataagcagag tcgttataaa gtttaacctg
      301 cgaattatcg gactggttaa aaatacgaat accaacgccg gttgccgcaa tcgcttgtcc
      361 tgtaccggta aaatcaccag ggttgcctgc tgtaagcggc gtacccactt tcaggtcgcc
      421 gtcgccgcca ggcgcttctg taccatcaaa acgaacggct gcttgagtgt acgttgccgg
      481 gcaattttca agtttaatac tgaacttctg tggcgacgcc agactaccta cacctgaaaa
      541 tgcattttta ctgactttac ccagtgggac aatttgatcg gctgtatcgg aggtaacttc
      601 acaagtagtg tcgacaattt caccgacgaa gttaacaaca ccatcttctg ccagtgcatg
      661 cccggccagc attagagatg ccagagtgaa tgcaccgcct ataatattac gcttcataaa
      721 aactccattg atataatata tcctttacca tgaatcatca cttatattaa taagtgtgtt
      781 tttgattcaa aggaatagta caccaaa
//
LOCUS       DAZXUK010000588          796 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZXUK010000588 DAZXUK010000000
VERSION     DAZXUK010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.597"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..199
                     /locus_tag="ACH3L8_004559"
                     /pseudo
     CDS             1..199
                     /locus_tag="ACH3L8_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          251 a          166 c          141 g          238 t
ORIGIN      
        1 cccacctgcc gggctaatcc accactccgc tcgaggttca cagtactgcg catacgatta
       61 ccgggtcata caggagcagt ctggtctgaa aacatcaatg tcgcgtaaag gtaactgtta
      121 cgacaacgct ccgatggaaa tcttctgggg aacgctgaaa aatgaaagcc tgagccacta
      181 tcgttttaag agccgggata tttcgtcagc atacgggaaa actgattgag gtcggcctca
      241 gttttgaggc ctttagccag ttcagccgca agggctttaa gcttcttttc gtccataatt
      301 tacctgtctc cgttgttgga gtaaacatat caaaaacagg caattacaca atttaaatta
      361 cagtctcaaa acaaacccaa cggtcaatat agtttaccag caagcttact agcaaagtgc
      421 taatcatttg gtatctttcg tgctattctc cttttacgta agtacttaag gggaacgtat
      481 ttactacttt taaagaaagc tccgttacta tctgaaatgc aatgagataa taaacgatta
      541 ctctcggtta gtatctcatg atcatttaca gactttgcat aatcgcgaat actatataac
      601 agagttttaa cattacagga tgaatagtct ctcttatcaa ctcctaataa aagaaggatt
      661 atggtcagtc gtttttgctg tcttatcata tattatttag tgcagttcct aacaaaaaca
      721 taacaatttt ctacaattga tcctgtgact acataaatat cttcaacaaa aatatttata
      781 tcagcaaaag agttat
//
LOCUS       DAZXUK010000589          796 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZXUK010000589 DAZXUK010000000
VERSION     DAZXUK010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.598"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(300..479)
                     /locus_tag="ACH3L8_004560"
                     /pseudo
     CDS             complement(300..479)
                     /locus_tag="ACH3L8_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            573..>796
                     /locus_tag="ACH3L8_004561"
     CDS             573..>796
                     /locus_tag="ACH3L8_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFT4087326.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          212 a          151 c          208 g          225 t
ORIGIN      
        1 cccggattgg tagggaaaag cttttttcat gcactatttt tgcaaatatt tctatatggg
       61 aagtggtttt gttacagatt aaatttataa agaaatgcat ttggggtgtt gggttgaaga
      121 gtagctggta gatgttattg tgccagcagg ggcaggggaa tgtgccgtat cgatatgctt
      181 tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaagcct gagttcgatg
      241 agggacgaac tcaggctggt gttatgtttt acagtaacac gcctcggcgc gttggctgat
      301 tagaactggt agacgatacc aactgccagt tggtcgtcgg tgttcagtcc atgagcgaga
      361 acataatcac tcttatcaag caggttgatc tgataagcgg tatatatgtt catattctta
      421 ttgaagtaat accaggtacc gatttcaata tagttcagac gatcagaatc tttgtaacct
      481 gtagactggc cccctgaatc tccagacaac caatatcact tatttaagtg atagtcttaa
      541 tactagtttt tagactagtc attggagagc agatgattga tgtcttagga ccggagaaac
      601 gcagacggcg tactacacag gaaaagatcg ctattgttca gcagagtttt gaaccgggaa
      661 tgacggtctc ccttgttgcc cggcaacacg gtgtggcagc cagccagtta tttctctggc
      721 gcaagcaata ccaggaggga agtcttactg ctgtggccgc aggagaacag gtcgttcctg
      781 cctctgaact tgctgc
//
LOCUS       DAZXUK010000590          788 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZXUK010000590 DAZXUK010000000
VERSION     DAZXUK010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.599"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(83..>788)
                     /locus_tag="ACH3L8_004562"
     CDS             complement(83..>788)
                     /locus_tag="ACH3L8_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005078762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087327.1"
                     /translation="CEGNGTQGDSSTGGAQLAINGLNGSCALVLNNPYFEANAGGADL
                     AIDNTGTRPVTVVINGGNFHRVSSARYTHTNIQVTSSGGGKVTVILNGTTFQSAGDYQ
                     PSAERPYWITGANCEVVDIGCVFTETTSKVTSASALSVTRSGKINANGSIDVATGVSS
                     VNVVSTGVYDVSFSHPLAAVASGYIVQITPISAPDSVSCDVTYIGVDTFRVTLRNTLS
                     GAGISSSFAFSITRLI"
BASE COUNT          228 a          203 c          151 g          206 t
ORIGIN      
        1 tacccacatt aacaaacaac aatcaataaa caataaacat aagccgccct acatatatgt
       61 ccaaatgggg cggcgaatgt atttatatta gtctggtaat gctaaatgca aacgaactgg
      121 agattcctgc cccgctaagc gtattgcgca gggtcaccct aaatgtatct acgccaatat
      181 aagtaacatc acaactaact gaatcaggcg cagatatagg tgttatctgc acaatataac
      241 cgctagcaac tgctgcgagc ggatgagaaa agctaacatc atacacacct gtagaaacca
      301 cgttaaccga tgaaacacca gtagcaacgt cgatactacc attggcgtta atttttcccg
      361 agcgtgtgac tgacaatgca gaagccgatg ttaccttact ggttgtttct gtaaaaacac
      421 acccaatgtc aactacctcg caattagctc ctgttatcca gtatggtcgt tctgcacttg
      481 gctgataatc ccctgcgctc tggaacgttg tgccgttgag tatgactgta acttttcccc
      541 cacctgagct tgttacttga atattcgtgt gcgtgtatct tgcactgctc acgcgatgaa
      601 aatttccacc gtttataacc acagttacag ggcgcgtccc agtattatct atagcaaggt
      661 ctgccccccc ggcattagcc tcaaagtatg ggttgtttaa taccaaagcg cacgaaccgt
      721 taaggccatt tatagctaat tgcgcaccac ctgtactgga atccccctga gtaccatttc
      781 cttcacaa
//
LOCUS       DAZXUK010000591          787 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZXUK010000591 DAZXUK010000000
VERSION     DAZXUK010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.600"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            87..>787
                     /locus_tag="ACH3L8_004563"
     CDS             87..>787
                     /locus_tag="ACH3L8_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFT4087328.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQW"
BASE COUNT          255 a          150 c          154 g          228 t
ORIGIN      
        1 ttttagcaag ctgttagtaa ccaaccgtta ctaataattt taattcttaa tactttgttc
       61 attcaataaa tgagttgaga gaatatatga ataaaatata ctcccttaaa tatagtgctg
      121 ccactggcgg actcattgct gtttctgaat tagcgaaaag agtttctggt aaaacaaacc
      181 gaaaacttgt agcaacaatg ttgtctctgg ctgttgcagg tacagtaaat gcagcaaata
      241 ttgatatatc aaatgtatgg gcgagagact atcttgatct tgcacaaaat aaaggtattt
      301 tccagcccgg agcaacagac gtaacaatca ctttaaaaaa cggagataaa ttctctttcc
      361 ataatctctc aattccggat ttttctggtg cagcagcgag tggcgcagct accgcaatag
      421 gaggttctta tagtgttact gttgcacata acaaaaagaa ccctcaggcc gcagaaactc
      481 aggtttacgc tcagtcttct tacaaggttg ttgacagaag aaattccaat gattttgaga
      541 ttcagaggtt aaataaattt gttgtggaaa cagtaggtgc caccccggca gagaccaacc
      601 ctacaacata ttctgatgca ttagaacgct acggtatagt cacttctgac ggttcaaaaa
      661 aaatcatagg ttttcgtgct ggctctggag gaacatcatt tattaatggt gaatccaaaa
      721 tctcaacaaa ttcagcatat agccatgacc tgttaagtgc tagtctattt gaggtcaccc
      781 aatggga
//
LOCUS       DAZXUK010000592          786 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZXUK010000592 DAZXUK010000000
VERSION     DAZXUK010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.601"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            36..786
                     /locus_tag="ACH3L8_004564"
                     /pseudo
     CDS             36..786
                     /locus_tag="ACH3L8_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          231 a          162 c          196 g          197 t
ORIGIN      
        1 attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa tacccttttg
       61 aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac aggatcatct
      121 cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc tatgaaaaac
      181 atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca gagttgcgta
      241 ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct gctgctcact
      301 ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa gagcgcggag
      361 aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata tcagttgatc
      421 cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag gatcttgaaa
      481 ggcaagttcg atttcttgaa acgcggcttg tgtatctaaa aaagctgaaa gccttagctc
      541 atcccacgaa aaagtgaaag tactcaacga gctaaggcag ttttatcctc ttgatgagct
      601 tctcagggct gcggagatac cgcgcagtac gttttattac catctaaagg ctctcagcaa
      661 gcctgacaag tatgcggacg ttaaaaagcg tattggtgag atttatcatg agaatagagg
      721 ccgatacgga taccgtaggg taacgctgtc tcttcatcga gaagggaaac agattaacca
      781 taaagc
//
LOCUS       DAZXUK010000593          783 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZXUK010000593 DAZXUK010000000
VERSION     DAZXUK010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.602"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            103..>783
                     /locus_tag="ACH3L8_004565"
     CDS             103..>783
                     /locus_tag="ACH3L8_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFT4087329.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITILEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLP"
BASE COUNT          273 a          149 c          133 g          228 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaatactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcatc
      661 cccccctttc cagataagct tgaagggctg gctctggcta ataattttat agaacaacta
      721 ccggaattac cttttagtat gaacagggct gtgctaatga ataataatct gacaacactt
      781 ccg
//
LOCUS       DAZXUK010000594          781 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZXUK010000594 DAZXUK010000000
VERSION     DAZXUK010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.603"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..666)
                     /locus_tag="ACH3L8_004566"
     CDS             complement(<1..666)
                     /locus_tag="ACH3L8_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFT4087330.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(656..>781)
                     /locus_tag="ACH3L8_004567"
     CDS             complement(656..>781)
                     /locus_tag="ACH3L8_004567"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HFT4087331.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          251 a          203 c          177 g          150 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt
      661 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc
      721 atttatcaaa tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca
      781 t
//
LOCUS       DAZXUK010000595          774 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZXUK010000595 DAZXUK010000000
VERSION     DAZXUK010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.604"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(22..693)
                     /gene="queE"
                     /locus_tag="ACH3L8_004568"
     CDS             complement(22..693)
                     /gene="queE"
                     /locus_tag="ACH3L8_004568"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFT4087332.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          187 a          206 c          172 g          209 t
ORIGIN      
        1 ataacgctta taaatgtgta atcaggcaat atttaaatat ttatgtgttt gcatcgacaa
       61 acgccaatta cgcgcaatac aggtttcaat gcacaatcgt gtggcatcat ccttttggct
      121 aatcggctgc agtgcaatga ctcgcggttt atcatcggtc agcgttgcca gtagttcatc
      181 cagtgcttca atatcgcgta cgcgccccac tggatgcttg atttcgttgg ctcgctccag
      241 tgcctgtgac aacacttcat agccgccgcg catgttcagc tttggcgata cggtaaccca
      301 ggtattcggt gtgcagcgta cttcatgagt tccactggtt tcgatctggc agctaaaacc
      361 gttcttttcg agcaggtcag tcagtggcag caaatcatga atgcaaggct caccacccgt
      421 aatcaccaca tgccgcgcgg tatatccctg acgaccaatg acagccagca agtcttcact
      481 gctcgccgcc ccccacttat cactctcttt ggtcttcgcc agaatgctga aaagggagac
      541 ttcccgatcc tcaagctttt cccaggtgtg tttggtgtcg caccaggcac agccaaccgg
      601 gcatccctgt aaacgaataa aaatggcggg aacgccggta aagtaaccct caccttgcag
      661 ggtctggaac atctcgttaa tcgggtactg catagcattc tctgtgaagt ggataattgt
      721 taattattgc agatcctgcc gcaacaatca tgtcttatta acattctgtt acat
//
LOCUS       DAZXUK010000596          774 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZXUK010000596 DAZXUK010000000
VERSION     DAZXUK010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.605"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..185
                     /locus_tag="ACH3L8_004569"
     CDS             <1..185
                     /locus_tag="ACH3L8_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HFT4087333.1"
                     /translation="HNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYENAEM
                     IKLLLKYGATSDNKYI"
     gene            complement(289..438)
                     /locus_tag="ACH3L8_004570"
                     /pseudo
     CDS             complement(289..438)
                     /locus_tag="ACH3L8_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            514..>774
                     /locus_tag="ACH3L8_004571"
     CDS             514..>774
                     /locus_tag="ACH3L8_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFT4087334.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHF"
BASE COUNT          268 a          138 c          144 g          224 t
ORIGIN      
        1 ctcataattc aagctgtaaa gtacttgagt attttatcaa taagggattg gttgatgtaa
       61 acacaaagtt caaaaaaact aatagtgggg attgtatgtt ggataacgca ataaaatatg
      121 agaatgcaga aatgataaaa ctattattga aatatggtgc aacatctgac aataaatata
      181 tttaatcaaa attgaatatc gtttagcaaa aggcagtatt tgcacaaaat atcgatatat
      241 tgtttattgt cagtatggct caatgtgata atagttggaa agtttgatgg gtttcgcccc
      301 gttgtagcgg tagtcgaact ggaaggtctt caggcactgc ttacagcgat agagcagccc
      361 ccctgaactg gaatggccgt tccggatacc cccctgagtt tcagagtaac ggtgacaaac
      421 cacatcaatc tttgccatca atcatccaaa gggcaaagag tacaacaaca ctaagtctgc
      481 gtcacaaccc atcaatgaaa ggaatatata catatgccat cagtaaattt aatcccatca
      541 aggaaaatat gtttgcaaaa tatgataaat aaagacaacg tctctgttga gacaatccag
      601 tctctattgc actcaaaaca attgccatat ttttctgaca agaggagttt tttattaaat
      661 ctaaattgcc aagttaccga tcactctgga agacttattg tctgtcgaca tttagcttcc
      721 tactggatag cacagtttaa caaaagtagt ggtcacgtgg attatcatca cttt
//
LOCUS       DAZXUK010000597          762 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZXUK010000597 DAZXUK010000000
VERSION     DAZXUK010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.606"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(56..132)
                     /locus_tag="ACH3L8_004572"
     tRNA            complement(56..132)
                     /locus_tag="ACH3L8_004572"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96..98),aa:Ile,seq:cat)
     gene            complement(322..>762)
                     /locus_tag="ACH3L8_004573"
     CDS             complement(322..>762)
                     /locus_tag="ACH3L8_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFT4087335.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          191 a          210 c          145 g          216 t
ORIGIN      
        1 cctcgcaccg tcaacttaga aggttgatgc tctatccgga tgagctagtg gtggttggtg
       61 gcccttgctg gacttgaacc agcgacctgg cgattatgag tcgctcgctc tgaccaactg
      121 agctaaaggg ccggaggcag aataataacc atatgtcatc acatctgcaa actcatctga
      181 tcaccagcac gtttaacgtc ctgtgccgtt tttcaggtat aaaaaaaacc gcataaagcg
      241 ggctctttca aatgtccatc tctgctattc gcctcgcggt acagctttgc gaagcgtaca
      301 agaattgaag cagtttttac gtcaaaaagc aataactttt ttctctatac caaaagccat
      361 aaccattggt ttgtacaaaa taaattctgc cacctttagc caatgctcaa tgcgtctttc
      421 acaggttctt aaactccatt tcgggtgtgc atcattcagc agttcagcca ttttgcattt
      481 ggtcatcccc cgcccctcat accgttgccg aaggacgcta atcaatcctg gatgctctgc
      541 cagcacctca cttatgaccc gatcaataca taacgcctct gcatcagtac aatgcgccag
      601 ccagctcttt tgcttaccgt taatcatatc ccgcaaaaaa gcctcaagtt cagacttgtt
      661 cagacctgct tttttcatcc tccggagcgc ctcgttaatt gccgtttttg tcagcttttt
      721 agaggtcaac atcaggttaa acatattccc cgtcttaccg cc
//
LOCUS       DAZXUK010000598          762 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZXUK010000598 DAZXUK010000000
VERSION     DAZXUK010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.607"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..525
                     /locus_tag="ACH3L8_004574"
     CDS             <1..525
                     /locus_tag="ACH3L8_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFT4087336.1"
                     /translation="VYVGDSALNLKGLVTLDGVPVNNAAKTWATSTPDEIRASINQVL
                     SDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAGNQSLLTNLQTNTISYHQNGVPLN
                     IRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPLQSVPVQYRGLYQIVTYYGKLGAV
                     EPVYKETISYVDGI"
     gene            complement(522..>762)
                     /locus_tag="ACH3L8_004575"
     CDS             complement(522..>762)
                     /locus_tag="ACH3L8_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087337.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
BASE COUNT          181 a          204 c          203 g          174 t
ORIGIN      
        1 gtatatgttg gcgattccgc attaaacctg aaaggccttg ttaccctgga cggcgtgcct
       61 gtcaacaacg ctgccaaaac gtgggcaacc tcaacaccgg acgaaatccg cgcaagcatt
      121 aaccaggtgc tgtctgatgc gtgggccgct tctggttact ctgtggttcc gcgtgatttg
      181 ctgatcccgc ctgagcagtt tgctctgttg tccagcatca tcgtttcatc tgcaggtaac
      241 cagtccctgt tgacgaacct tcagaccaac accatcagct atcaccagaa cggtgttccg
      301 ctgaatatcc gcgcggttaa atggctgaaa ggccgtggtg tggggaataa ggatcgcata
      361 gttgcgtaca ccaacgataa aaaatacgtc cgctacccgc tggttccgct tcagagcgtg
      421 ccggtgcagt atcgcggtct gtatcagatc gtcacttact acggcaagct gggtgcagtc
      481 gagccagtgt acaaagaaac catttcgtac gttgatggca tttaacagcc acatggcccc
      541 ctggcggggc cattaaggat gacccgatgg caaaaaatga tgcagtaata cacgtacata
      601 ccccgtttgt gctcacgctt cccgacggtt ctcggcgcga gtttgttaaa ggccgtcatg
      661 ttgtggagga agacgttgcc acgcactggt tcactcgtgc gcacgcggaa gtatccgttg
      721 gcaaagccac agacgcgcgt aacgaggtaa aaaatgccaa ag
//
LOCUS       DAZXUK010000599          748 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZXUK010000599 DAZXUK010000000
VERSION     DAZXUK010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.608"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            119..271
                     /locus_tag="ACH3L8_004576"
                     /pseudo
     CDS             119..271
                     /locus_tag="ACH3L8_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(553..>748)
                     /locus_tag="ACH3L8_004577"
     CDS             complement(553..>748)
                     /locus_tag="ACH3L8_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087338.1"
                     /translation="GESSLYCDNVTVQYCAFPKLTTTELFWDLNAIKWLVEGRGDDVS
                     PFENIPVPDLTYNKEAWQFG"
BASE COUNT          226 a          144 c          143 g          235 t
ORIGIN      
        1 atattcagta tttgcagtat ttattggaaa tacgttctta atattcttga tgggactaat
       61 cattttaatc tctatactca aatataaaca ttaccatgaa caatataatt gttcctaagt
      121 ggaatgtctc cacggagaac actttatcta tcgggaaata gtgcgggcaa cagtgtttaa
      181 ttatatcgag tgcaattaca atcgctggcg acggcaccgt tggtgtggcg gcctcagtcc
      241 ggaacaattt gaaaaccaga acctcgctta ggcctgtgtc catattacgt gggtaggctc
      301 acagagcctc acttacgact gcgtccatat tatgtgggta ggatcaataa agacgtttta
      361 tatgtgtctg cttgcagcca gaaaatttat accctgcagg tatcataaac atgcttgata
      421 acttaattgg ggctccctct tccttttatg gtgtaattgt ttgattgaat acttccgggg
      481 atttcagtat gaaatgaagt atatttaata tactttacaa aaagtttgta ttagtggaat
      541 aaggacaata gcttatccaa actgccaagc ctctttatta taagtaagat ctggcactgg
      601 aatattctca aaaggactaa catcatcacc acgaccttca accaaccatt ttattgcatt
      661 taaatcccag aatagttcag tagtggtcaa tttgggaaac gcacaatatt gtacggttac
      721 gttatcgcaa tagagtgaac tctcgccc
//
LOCUS       DAZXUK010000600          740 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZXUK010000600 DAZXUK010000000
VERSION     DAZXUK010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.609"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            198..>740
                     /gene="ospC2"
                     /locus_tag="ACH3L8_004578"
     CDS             198..>740
                     /gene="ospC2"
                     /locus_tag="ACH3L8_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFT4087339.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEI"
BASE COUNT          287 a          122 c          108 g          223 t
ORIGIN      
        1 atatatccaa aaacaaataa tactgttgca ataattcctt tcagtaaagg aagtgtgatt
       61 aaactcaggg gatttcagag tgaaaatcgt acaacatatt aaaatatgtt cgttataata
      121 catacaagaa aatattaagt tagataatat tattctaaat aactgcagat aaaaacgcac
      181 ataatggggg atgttttatg aaaatacctg aagcagtaaa tcatattaat gttcagaaca
      241 atattgatct tgttgatgga aaaataaatc cgaacaaaga tacaaaagca ttacaaaaaa
      301 acatatcatg cgtaacaaac tcatcctctt ctggcataag tgaaaaacat ttagatcact
      361 gtgctgatac tgtgaaaagc tttttaagga agtctatagc agcgcaaagt tatagtaaaa
      421 tgttctctca agggaccagt ttcaaatcat taaatctttc tattgaagcg ccatctggag
      481 cacggtcatc atttcggtca cttgagcacc tcgataaggt gtccagacac tatctttctg
      541 aaataataca aaaaactcat cctctttctt cggatgaacg tcacttgctc tctattatta
      601 ttaattctga ttttaacttc agacatcaaa gtaacgctaa cctgtcaaat aatactctta
      661 atattaagtc ctttgataaa attaagtctg agaatataca aacatataaa aacacattct
      721 ctgaggatat tgaagaaata
//
LOCUS       DAZXUK010000601          733 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZXUK010000601 DAZXUK010000000
VERSION     DAZXUK010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.610"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>733)
                     /gene="ipaH7.8"
                     /locus_tag="ACH3L8_004579"
     CDS             complement(<1..>733)
                     /gene="ipaH7.8"
                     /locus_tag="ACH3L8_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HFT4087340.1"
                     /translation="CSPSINSNSTSNEHYLRILTEWEKNSSPGEERGIAFNRLSQCFQ
                     NQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKELNADNNRLSVIPE
                     LPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLKFLHVYYNRLTTL
                     PDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPESFSQLDSSYRIN
                     ISGNPLSTRVLQSLQRLTSSPDYHGP"
BASE COUNT          228 a          110 c          163 g          232 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aag
//
LOCUS       DAZXUK010000602          729 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZXUK010000602 DAZXUK010000000
VERSION     DAZXUK010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.611"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..503
                     /locus_tag="ACH3L8_004580"
                     /pseudo
     CDS             1..503
                     /locus_tag="ACH3L8_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            507..666
                     /locus_tag="ACH3L8_004581"
                     /pseudo
     CDS             507..666
                     /locus_tag="ACH3L8_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          183 a          180 c          207 g          159 t
ORIGIN      
        1 acatcctgcg ccagtatgtg ctgatgcccg gtaaagtcca tgccgatgat atccccgttc
       61 cggtccagga gccgggcagc ggtaaaacaa ggactgcccg gctgtgggtc tacgttcgtg
      121 atgaccgtaa cgccggttcg gaaatgcctc cggcggtctg gttcgcgtac tcaccggacc
      181 ggaaaggtat ccatccacag aatcatctgg ccggttacag cggagtgctt caggccgatg
      241 cttacggtgg ttaccgggcg ttgtacgaat ccggcagaat aacggaagcc gcatgtatgg
      301 ctcatgtccg gagaaaaatc cacgatgtgc atgcaagagt gccaaccgac atcaccacgg
      361 aagccctgca gcgtatcggt gaactgtatg ccatcgaggc agaagtccgg ggatgttcag
      421 cagaacagcg tctggcggca agaaaagcca gagccgcgcc actgatgcag tcactgtatg
      481 actggataca gcaacagatg aaaattcatt cgctgaaaat ggaatgtctc cacggagaac
      541 actattatcc atcgggaaat agtgcgggca acagtgttta gttatatcga gtgcgattac
      601 aatcgctggc gacggcaccg ttggtgtggt ggtctcagtc cggaacaatt tgaaaaccag
      661 agcctcactt acgactgtgt ccatattatg tgggtaggat caataaagac gttttatatg
      721 tgtctgctt
//
LOCUS       DAZXUK010000603          716 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZXUK010000603 DAZXUK010000000
VERSION     DAZXUK010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.613"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..636
                     /locus_tag="ACH3L8_004582"
     CDS             <1..636
                     /locus_tag="ACH3L8_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFT4087341.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT          226 a          146 c          180 g          164 t
ORIGIN      
        1 ccgttatcta aaacagaatt caatgcatta atggaaagtg aaaaaggaca gagccaaaac
       61 ttgtggaaat ttgccgttta ctccgggctt cgtcacgggg aactggcagc tctggcgtgg
      121 gaggatgtgg atttcgagaa gggaattgtg aatgtcagaa gaaacctgac gatacttgat
      181 atgttcggtc ccccaaaaac aaatgccggg atccggacgg tagcattact gcagccggct
      241 cttgaagcac tgaaggagca atacaaactg accgggcatc atcgcaaaag cgaaatcact
      301 ttttatcatc gggagtacgg cagaactgaa aaacaaaaac tgcattttgt tttcatgccc
      361 agggtgtgta acgaaaaaca gaaaccttat tactcggtaa gcagtttggg tacaagatgg
      421 aatgcagcag taaaacgtgc tggtattcgc cgccgtaatc cgtaccatac gcgacatact
      481 tttgcctgct ggctgttgac ggcaggagcg aacccggcat ttatagccag ccagatgggg
      541 catgaaactg cgcaaatggt gtatgaaatt tacggtatgt ggattgatga catgaacgac
      601 gaacagatag ccatgttgaa tgcgcggtta tcgtagttgc aaagtttgcc cccaatttgc
      661 cccatttagt accagagaac tgaaataatg caagaaaatc aacaaattac aaagaa
//
LOCUS       DAZXUK010000604          713 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZXUK010000604 DAZXUK010000000
VERSION     DAZXUK010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.614"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            163..648
                     /locus_tag="ACH3L8_004583"
     CDS             163..648
                     /locus_tag="ACH3L8_004583"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087342.1"
                     /translation="MRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMPETKFTLLNVT
                     K"
     gene            645..>713
                     /locus_tag="ACH3L8_004584"
     CDS             645..>713
                     /locus_tag="ACH3L8_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087343.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFK"
BASE COUNT          233 a           90 c          153 g          237 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg gcctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct ttgggaacga taccttaaaa acgcaactat aacgttgaat
      601 atcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aag
//
LOCUS       DAZXUK010000605          706 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZXUK010000605 DAZXUK010000000
VERSION     DAZXUK010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.615"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            88..153
                     /locus_tag="ACH3L8_004585"
                     /pseudo
     CDS             88..153
                     /locus_tag="ACH3L8_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            381..629
                     /locus_tag="ACH3L8_004586"
     CDS             381..629
                     /locus_tag="ACH3L8_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFT4087344.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          226 a          150 c          160 g          170 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat
      661 cattcaatac tcgcactatc gaaagtttac cagccaaccg cagcac
//
LOCUS       DAZXUK010000606          704 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZXUK010000606 DAZXUK010000000
VERSION     DAZXUK010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.616"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..443)
                     /locus_tag="ACH3L8_004587"
     CDS             complement(<1..443)
                     /locus_tag="ACH3L8_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFT4087345.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          214 a          172 c          131 g          187 t
ORIGIN      
        1 tcatctggat aatcggcatg agcaataaca tgcttgccgg tgcgactgtg tacttcaaga
       61 ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg ttgattatct
      121 gccagcgcaa taaaccagtc gccgccattc atcaaccaca aagacatctg ctggaatgcc
      181 agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca atgttgttcc
      241 agtaactgca gacaacgtaa actttgcggc ccacgatcga taacgtctcc cactgagata
      301 agtaaatctc gccacggatc aaaacgacaa tgccataatt tgcggcgcaa ctgctcaaga
      361 caaccgtgta tatcgccaga aagccagata tgtcgccatt gatgacccgc aattctctga
      421 taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc acataatctt
      481 aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg gttgactata
      541 aagtcagcga aggaaatgct tctggctttt aacagataaa aagagaccga acatgattcc
      601 tgttttcgtc aacaaacaat aaaacccttt aaaattaatg cgttgaataa atttacgttc
      661 atcttttaat ccctgacacg taccattgca tattaataca ttca
//
LOCUS       DAZXUK010000607          702 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZXUK010000607 DAZXUK010000000
VERSION     DAZXUK010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.617"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(105..290)
                     /locus_tag="ACH3L8_004588"
     CDS             complement(105..290)
                     /locus_tag="ACH3L8_004588"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFT4087346.1"
                     /translation="MLPLSVNLMSSGASLAARLQQHWLWYSYNTKTGGVLAYTFGPRN
                     DETCRELLVLFTPFCIY"
     gene            complement(315..587)
                     /locus_tag="ACH3L8_004589"
     CDS             complement(315..587)
                     /locus_tag="ACH3L8_004589"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HFT4087347.1"
                     /translation="MATVTVHCPRCHSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKPGVKEQIVEMAHNGAGGRDTARTLKIGINTVIRTLKSSRPGG"
BASE COUNT          196 a          173 c          160 g          173 t
ORIGIN      
        1 aacaaatatc cctctgatat gacaacaaaa aacggagccg gattcctgtt tttgtgaact
       61 gcagctattc gtgtctaaaa agtgatccac ttaccaccaa cggcctaata tatacagaac
      121 ggagtaaaca ggactaacag ttcccgacag gtctcatcat tccgtggccc gaaagtgtaa
      181 gccaacacac cgccagtttt agtgttgtac gaataccaga gccagtgttg ttgcagcctt
      241 gctgccaacg aagcgccact gctcatcaag ttcacagata agggcaacat cagcgtgagc
      301 gactggcgca gatgttatcc gccggggcgg gagcttttta atgtccgtat gacggtattg
      361 atgccgattt tcagtgtcct ggcggtatca cggccccctg cgccgttatg tgccatttca
      421 acgatctgct ccttaacgcc gggttttctg gcttcataag agtaagtgag ctggaaaaca
      481 cgtttacatg agcgacaacg gaaacgctca tgccgggaac aactacggcc atgtcgataa
      541 acttcatcgg aatgacagcg cggacaatga acagtaacgg tagccatgga aaaccttaaa
      601 taccagtaga ttaccagaaa cttctgtcga ttgaagacat cacccgaagt ttgaattcat
      661 tcgaatactt tttacgaggt tcttttcgcc agtcctgtaa tt
//
LOCUS       DAZXUK010000608          697 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZXUK010000608 DAZXUK010000000
VERSION     DAZXUK010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.618"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..223)
                     /locus_tag="ACH3L8_004590"
     CDS             complement(<1..223)
                     /locus_tag="ACH3L8_004590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFT4087348.1"
                     /translation="MQLMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDADNELFHYT
                     IKKAYTPYNIHTYKTEEVVNQRNIKVKNMT"
     gene            complement(230..>697)
                     /locus_tag="ACH3L8_004591"
     CDS             complement(230..>697)
                     /locus_tag="ACH3L8_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFT4087349.1"
                     /translation="VTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT          220 a           95 c          132 g          250 t
ORIGIN      
        1 aggtcatgtt tttaactttt atattcctct ggtttacaac ttcctctgtc ttgtaagtgt
       61 gtatattata tggggtatat gcttttttaa tagtatagtg aaacaattcg ttatccgcat
      121 ctattccgtc agagtgaact tcaaatgcac caggcgaaag aattgccagg ccagtattaa
      181 gcttaagtgc tggggaaaac aaattaaata acatgagctg catagtaggt caattgttct
      241 tcccagaaac atttaaaaaa tttaagtttt tgctctgttt tattatgggg aataaagttc
      301 caatatcgga tgtattatta gtatcaatgt gggttatttt attattgtcc agtcttaaac
      361 ttttgataga tggaagataa gtcaggaaat caagatttat atatttcaac tggttgttag
      421 cagcgtttaa tgtctgaaca ttgcggaagt tgctatagaa tattttaatt tcagaaagca
      481 aattgttgtt aataagtaac gtattcaagt tgggaaataa gacattgtgt aaacttgtta
      541 gtttattatg ggatagatcc agcagtaaga gttcattttt attacctgca acaatatcag
      601 ttaattgatt atggctgaga ttcaggtatt gcagccattc gcaagattct aattgcacaa
      661 actctagttg attatgtgcc gcactaaaat aagtaac
//
LOCUS       DAZXUK010000609          695 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZXUK010000609 DAZXUK010000000
VERSION     DAZXUK010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.619"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            32..493
                     /locus_tag="ACH3L8_004592"
     CDS             32..493
                     /locus_tag="ACH3L8_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFT4087350.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..>695)
                     /locus_tag="ACH3L8_004593"
     CDS             complement(534..>695)
                     /locus_tag="ACH3L8_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFT4087351.1"
                     /translation="ILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGL
                     AARQHVKKS"
BASE COUNT          204 a          152 c          159 g          180 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaat
//
LOCUS       DAZXUK010000610          686 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZXUK010000610 DAZXUK010000000
VERSION     DAZXUK010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.620"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..663
                     /locus_tag="ACH3L8_004594"
     rRNA            <1..663
                     /locus_tag="ACH3L8_004594"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          172 a          162 c          204 g          148 t
ORIGIN      
        1 cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg cccgcacaag
       61 cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt cttgacatcc
      121 acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt gctgcatggc
      181 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
      241 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      301 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      361 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      421 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      481 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      541 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      601 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcacctcc
      661 ttaccttaaa gaagcgttct ttgtag
//
LOCUS       DAZXUK010000611          684 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZXUK010000611 DAZXUK010000000
VERSION     DAZXUK010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.621"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..474)
                     /gene="mxiG"
                     /locus_tag="ACH3L8_004595"
     CDS             complement(<1..474)
                     /gene="mxiG"
                     /locus_tag="ACH3L8_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HFT4087352.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIID"
     gene            complement(483..>684)
                     /gene="ipgF"
                     /locus_tag="ACH3L8_004596"
     CDS             complement(483..>684)
                     /gene="ipgF"
                     /locus_tag="ACH3L8_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFT4087353.1"
                     /translation="PCLSVHYAAKLLNEFMMMYGRGWEAVGAYNAGTSPKKKKERLKY
                     AEDIYRRYLRIAAESKQNNRRI"
BASE COUNT          208 a          146 c           90 g          240 t
ORIGIN      
        1 atctataatt tcagcaattt cttttacttc atctttcttt aaagagaggg aaaaaattat
       61 aattaacaca acaataacag caaagaaaaa aaacaccgag tggttcttat acattccgtt
      121 tagtatatgg cctctcgatt tatcctccct catattttta aggtgaaaag aaatcccgtc
      181 atattcaaat gaattaaatt gtgaattgat atcaatttgc tcaccttttt catcactaaa
      241 ttgacagata ttatctttgc ttatggtcaa ataaattata ccatccgatt tactatctgt
      301 tacaggaata atattttctg ggaaattgtc gtttccaaac tcaagtgttt ctatcgtccg
      361 tccgagaaca attaagttat tcccataata taacggaaac tcatccccga caccgtttgt
      421 gagtttcact aataacctga atggtgcaag atttgagttc tttgcctcag acataaatcc
      481 tcttatatcc ttcgattatt ctgcttgctc tctgctgcta tccgcaaata gcgtctatag
      541 atatcctctg cgtactttaa tctttctttt ttctttttcg gagacgttcc tgcattatag
      601 gctccaacag cctcccaccc tcttccatac atcatcataa actcatttaa taactttgct
      661 gcataatgta cagaaagaca tgga
//
LOCUS       DAZXUK010000612          684 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZXUK010000612 DAZXUK010000000
VERSION     DAZXUK010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.622"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..525
                     /locus_tag="ACH3L8_004597"
                     /pseudo
     CDS             1..525
                     /locus_tag="ACH3L8_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            589..>684
                     /locus_tag="ACH3L8_004598"
     CDS             589..>684
                     /locus_tag="ACH3L8_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFT4087354.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRL"
BASE COUNT          190 a          140 c          165 g          189 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagcg ttgcaacacg tctc
//
LOCUS       DAZXUK010000613          670 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZXUK010000613 DAZXUK010000000
VERSION     DAZXUK010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.623"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..574)
                     /locus_tag="ACH3L8_004599"
     CDS             complement(<1..574)
                     /locus_tag="ACH3L8_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFT4087355.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVF"
BASE COUNT          187 a          160 c          116 g          207 t
ORIGIN      
        1 aaaaaacaag cgtcgtcagc cagcacactg ctaacacggt aagaacgcta ataaaaacac
       61 tccactgctt cgttctatac cattcaatat ttttgtcagt atgttttatt gggagcagct
      121 cttcagtaga gaattcctgg ctgttatcat tctcaggaat gtttgcatca gtgtttactt
      181 ccctggcctc gctatcgttg gtatagctac tactgtcttc atcactgtca cgctcattct
      241 cacagacatg tttctcgacc tttcccagat atctgacgcc ttttcgcgga atggttttaa
      301 ttagagaact cgacaaacct gcgacgtcta atgcctttct aagcatgtaa atctgcccat
      361 aataactgct gtcgcttacc gaacctttct gctcccgcca aacctcattg ataatatgtt
      421 gcttatcgga ttctccctgt agtaaaagga gtaaaaatcg atagccgttt tcagacagat
      481 tgattacatt gcctgtactg gtggtgatac gtcgagacgc tgggtaaaac ttaactccct
      541 gccctaaaga aaaataaaaa cggtcatcgc tcataagtac cgacccctgt tttcttcttc
      601 acatgactac tcattacaac tttgtatgaa gtaagatgca aaatcatatt gtttacatta
      661 agttaagcaa
//
LOCUS       DAZXUK010000614          670 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZXUK010000614 DAZXUK010000000
VERSION     DAZXUK010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.624"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..273)
                     /locus_tag="ACH3L8_004600"
     CDS             complement(<1..273)
                     /locus_tag="ACH3L8_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087356.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIV"
BASE COUNT          198 a          138 c          137 g          197 t
ORIGIN      
        1 aacaatatca ggcgtgcctg cggaacggtc cttaccatgc tgcagtaacc ggaaataaac
       61 ttttgggcgt aagatgacat tgacagtttc gacatcgtct ttttctccct taaaaaattt
      121 attgacaatc gcttcgtcaa gacgtcccgc cgctatccca tgccagtgag tgaaattttg
      181 ggcgtctttc cgttcaaaac agccctttcc tgactcagca tctgcaagcg agttgcgcca
      241 gtatgaggca aaccttaaag cattttcatc cattaccagt cctgtgactc aatgaaatcc
      301 attgatttat cgaatataaa accaccgtat ggacaactat tgtccatatt aaaaaagttg
      361 gatgcaatgt tttgtttata attcgatatc aaatgtactg gttgtacgag gttcagaaca
      421 caagcacatt caacagtacg tcaggactat tgatttcata acagcgaata caatttttta
      481 ctgtttataa tggctgcctg tgttggacgg ggataccagt tcaaccgaaa acgccagagg
      541 aggggattac ccgctggcag ggtaaatctg tggtttttga tgccagctat aacgtgtttg
      601 acgataaagt attacgaaag aactttaatg agttcatgga gtaattcgtt aaagtaattt
      661 acaacgctat
//
LOCUS       DAZXUK010000615          668 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZXUK010000615 DAZXUK010000000
VERSION     DAZXUK010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.625"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>668
                     /locus_tag="ACH3L8_004601"
     CDS             <1..>668
                     /locus_tag="ACH3L8_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HFT4087357.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DAZXUK010000616          665 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZXUK010000616 DAZXUK010000000
VERSION     DAZXUK010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.626"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(160..>665)
                     /locus_tag="ACH3L8_004602"
     CDS             complement(160..>665)
                     /locus_tag="ACH3L8_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFT4087358.1"
                     /translation="PVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDL
                     KELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDP
                     STPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKL
                     VERLMQL"
BASE COUNT          175 a          165 c          146 g          179 t
ORIGIN      
        1 caccagcgcg tttaacgccc tgtgccattt ttcaggcaca aaaaaacccg cataaagcgg
       61 gttctttcaa gtgtccatgt ctgctattcg cctcgcgata cagctttgcg aagcttaccg
      121 taattgaagc agtttatgtg caaaaatgca agaacttttt taaagctgca tcaacctttc
      181 caccagttta tctctgcgaa caacaaacca accattggct ctcgccagtt ccagccatga
      241 ctcaagggaa ataacaatat catcatcccg taactgaatt gtggaaacag tgacaccgcc
      301 tctctgataa cagagaactc gcgtgtcgta acttttctgg catgaaactg gcgttgacgg
      361 atccctttga ctgaaatagc agtcttccag tttttcgaac acatcccacg cctgatcggt
      421 ttcgagcatt ttgccgtggc gtgctgcgcc tcgttctgtc cagaggatga gggagcgggc
      481 tttgggtgaa actggatttt gtgagtagtt taaagcgacc cgcaattctt taaggtcatt
      541 accaacaact ttgaaaaagt gtttcccttc aacgaagcgt actttgttct catgatgatt
      601 ctggcgaata cgcaccggct cagtgccgta aagctgcgcc aaaagttcgg tggtaataac
      661 aggaa
//
LOCUS       DAZXUK010000617          660 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZXUK010000617 DAZXUK010000000
VERSION     DAZXUK010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.627"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..131
                     /locus_tag="ACH3L8_004603"
     CDS             <1..131
                     /locus_tag="ACH3L8_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFT4087359.1"
                     /translation="ERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            246..>660
                     /locus_tag="ACH3L8_004604"
     CDS             246..>660
                     /locus_tag="ACH3L8_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFT4087360.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPS"
BASE COUNT          168 a          177 c          171 g          144 t
ORIGIN      
        1 ttgaacggtg ctttgggcgg ctgaaagaat atcgccgcat cgccacgcgt tacgacaaaa
       61 cggcgagaaa ttacctggcg atggtgaaac tgggctgcat ccgactcttt tatcaacgct
      121 tacgtaatta agggacacag cctaaatagc tgcgcggaat agtagatccc tttgagggaa
      181 cttagcccgg attgtgcgat ctgatcaatc gccaaatcaa aacaaatcac caaccggact
      241 gagcaatgcc gatcatagca ccaatttccc gtgacgaacg acgcctgatg cagaaagcca
      301 tccataaaac acacgataaa aattatgccc gcagactgac tgccatgctg atgctgcacc
      361 ggggcgaccg tgtcagcgac gttgccagaa cgctctgctg cgcccgttcc tctgttggac
      421 gctggattaa ctggttcacg cagtcgggtg ttgagggact gaaatcatta cctgccgggc
      481 gtgcccgtcg ctggccgttt gagcatatct gcacactgtt acgtgagctg gtaaaacatt
      541 ctcccggcga ctttggctac cagcgttcac gctggagtac agaactgctg gcaataaaaa
      601 tcaatgagat aaccggctgc cagttaaatg ccggaaccgt tcgccgctgg ttgccgtctg
//
LOCUS       DAZXUK010000618          656 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZXUK010000618 DAZXUK010000000
VERSION     DAZXUK010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.628"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>656
                     /locus_tag="ACH3L8_004605"
     CDS             <1..>656
                     /locus_tag="ACH3L8_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087361.1"
                     /translation="VLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRNLPSGVVP
                     AVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHR
                     YGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELS
                     WWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPL
                     P"
BASE COUNT          144 a          179 c          189 g          144 t
ORIGIN      
        1 gttctggttc cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc catgccggta
       61 ttccacaata cccgggtact ggtggagccg gaaccgaaaa gcatgcgtaa tctgccgtcc
      121 ggggtcgttc ctgccgttcg ccagccgcta gtggaagaca aaacattgct gccgtttttc
      181 agtaacgcac gggtgattcg tgctgctggt ggtgctggcg cattgtctga ctggctgttg
      241 cgccatatta aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt
      301 caccgttatg gtaccggcgc aatggtattg tgctggcact gcgacaacca gctgcgtgac
      361 cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac
      421 gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc
      481 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
      541 ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat cgtgccggga
      601 gagcagaccg ccaccagcat actgaagcag cgcacaaaaa atcttgcgcc gctgcc
//
LOCUS       DAZXUK010000619          650 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZXUK010000619 DAZXUK010000000
VERSION     DAZXUK010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.629"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="ACH3L8_004606"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="ACH3L8_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFT4087362.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT          166 a          127 c          147 g          210 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc
//
LOCUS       DAZXUK010000620          649 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZXUK010000620 DAZXUK010000000
VERSION     DAZXUK010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.630"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(491..>649)
                     /locus_tag="ACH3L8_004607"
     CDS             complement(491..>649)
                     /locus_tag="ACH3L8_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFT4087363.1"
                     /translation="ISKRYDDVEVIVKATSNDGLSVTRTADKDSAKTFVQETLKDTWE
                     SADEWFVH"
BASE COUNT          162 a          125 c          152 g          210 t
ORIGIN      
        1 gatatatagt ttgtgtaagc atattaactc catatgttat atattgaaga acttctgctt
       61 tacactatcc taaactggat cggtttaatg taaaaaaacg atactttttg ggggggggca
      121 atttttcctt aaggtatgaa aaaaggagct gaagctatat aatagctatt aatgccactg
      181 gtatcagtga atcatgtata taaaaaactg tgttaaggag gctgtccatt agcggttttt
      241 tattcttctg gaaccgcggc atctttgcgt atctttttgt cttctcaccg tctggtcggt
      301 gttttgctga gactgctaat ttcctgtctt tgctagtgct gtactggtac tgcccaatca
      361 tgattggcgg gggcgggcgg agttgagacc gcagacacgt cgtatgcaag aacgtgctgc
      421 ggctggctgg tgaacttccg atagtgcgag tattgaatga tttccagccg ttactgattt
      481 tacgtgctaa ttagtgaaca aaccactcgt cagcagactc ccaggtatct ttcagagttt
      541 cctgaacaaa agttttagct gaatctttat cggcggtgcg cgtaacagaa aggccatcgt
      601 tgctggtggc ttttacgatc acctctacat cgtcataacg tttactgat
//
LOCUS       DAZXUK010000621          649 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZXUK010000621 DAZXUK010000000
VERSION     DAZXUK010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.631"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..582)
                     /locus_tag="ACH3L8_004608"
     CDS             complement(<1..582)
                     /locus_tag="ACH3L8_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFT4087364.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAI"
BASE COUNT          176 a          102 c          125 g          246 t
ORIGIN      
        1 tatggctgag tttttaactg ggagattgtg attaataatt ttacgcagga attgaatttg
       61 ttcttctggt gaaattttta agctactttc gagccatgct tctgttaatc cgttgtttct
      121 ttctttatct ccagagaagt cttgatttcc ataatcaaaa tctttgagat aattcttgat
      181 tttatttaat ccaatttttt gggttatttc ttgcgaaacc caaacaacag aaaattgcat
      241 ccacgtcttt ggtgtatgat tgctgttcca gatctccatt cctttggggg ttttatccca
      301 tttgaatatg gttttctgat ctattatttc cgcatcaaat gccataagtg ataatgcgat
      361 cttgaaagtt gaatctggtg ccatttgcgt tgcacacttt gctttattga attgagcaat
      421 ttcagcgttt gtggatgcat cgtaaagtaa aaaacaacct tcagttcctt caaataatgg
      481 agatgcaaca gtagagatat ctgttgatgc actggcgctg ctgtagataa tatttgcaat
      541 tattaaaaaa atagcgaagt tgatatgtat tgtgtttttc ataataagta ttggtttggt
      601 aaagggctta attttaacgg ctaacaatta atgaggctcc gggttcgcc
//
LOCUS       DAZXUK010000622          645 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZXUK010000622 DAZXUK010000000
VERSION     DAZXUK010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.632"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..112)
                     /locus_tag="ACH3L8_004609"
                     /pseudo
     CDS             complement(1..112)
                     /locus_tag="ACH3L8_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            110..193
                     /gene="tnpB"
                     /locus_tag="ACH3L8_004610"
                     /pseudo
     CDS             110..193
                     /gene="tnpB"
                     /locus_tag="ACH3L8_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          180 a          131 c          164 g          170 t
ORIGIN      
        1 tcaatgaagg agccgatgac ttttcgtgga tttccacgga acgcgagaag cagattgttt
       61 tacgtgccag ccgcttgatg cgggtgcgga gggtcaggtt gttacgttca agatatagca
      121 tgtgctggat gaagatccct tttctggtca cctgtttatc tttcgtggcc gacggggtga
      181 cactgttaaa atgtaatgaa cgcatcccac acaattaaac acggagtagt atgatggtta
      241 aaagggtgaa actggcaggt gtggagcctc gcagctttcc ggcatcatac tgcccaatac
      301 gggatgcata aagcgtcctc agactggaga ctccacaaat gaaatataca ccggttggcg
      361 ttgatatggc aaaacatgtc attcagattc acttcatcaa tgagcacatg tcagcgccag
      421 tgatataaga cggtaatggg gatgcggacg atttcctgga ctctcagaag atgaaacatg
      481 tttactgaag aacaaaaaat tcgcgctatt gagctttatt tcaaatatgg taagaagctt
      541 gaggtgtact ggcaatagcg gacaccacca tttgttcttt ttctaagcag tcatctgata
      601 atattttatc ctgaaggctg ccggggagat attccccaga cgaga
//
LOCUS       DAZXUK010000623          644 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZXUK010000623 DAZXUK010000000
VERSION     DAZXUK010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.633"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            2..>644
                     /locus_tag="ACH3L8_004611"
     CDS             2..>644
                     /locus_tag="ACH3L8_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFT4087365.1"
                     /translation="MRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVTNSCGS
                     RACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVCRLAVE
                     NLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFLKDAIR
                     SRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYMSKKT"
BASE COUNT          152 a          163 c          200 g          129 t
ORIGIN      
        1 tctgcgcgat atcgaggttg aggctgtcac aaaaatgctg gcctgcggca cgcggatact
       61 gggcgtgaag gaatacatct gcgataaacc tgagtgcccc cacgtcagat acgtcactaa
      121 ctcatgcggc agccgtgcct gcccgtcctg cggaaaaaag gccacagacc tgtggatagc
      181 gacacagctg aatcgtcttc ctgactgcga ctgggtacac ctggtcttca ccctgccgga
      241 cacgcagtgg ccggtgttcg aaagcaaccg gtggctgctg aatgacgtgt gccgtctggc
      301 ggtggagaat ctgctgtatg ccgcccggaa acgggggcag gaacccggta tcttctgcgc
      361 catccacacg tatggccgtc gtctcaactg gcatccgcat gtacatgtgt ctgtaacctg
      421 tggaggtctg aataagcatg gtcagtggaa aaagctgagc ttcctgaaag acgcgatacg
      481 ttcgcggtgg atgtggaata tgcggcaacg gcttctgaaa gcgtggtcag aggggctggc
      541 gatgccggag tcgttgtcac atatcacgac ggaatcacag aggagaagcc tggtgctgaa
      601 agccggagga aaatactggc atgtgtacat gtcgaaaaaa acgg
//
LOCUS       DAZXUK010000624          636 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZXUK010000624 DAZXUK010000000
VERSION     DAZXUK010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.634"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            19..492
                     /locus_tag="ACH3L8_004612"
     CDS             19..492
                     /locus_tag="ACH3L8_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4087366.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          210 a          113 c          128 g          185 t
ORIGIN      
        1 ggagatgaac cctatgcaat gtcctggtta atatctcagt gtgctcatca gtgcactgat
       61 aataaaaaga cggagacaga tgccatttac gataaagttc ggagttctta tttgctttca
      121 tgtattctga aaaaaaacaa aaatgttggg cttattcttc atgcaccatc atttgtttct
      181 gtgtctgaaa aaatcgccag aattgtgatg gcaaattact cccgtaactg gagtaattcc
      241 gagttggcca gtgcggtttt aatgagtgaa tcttctttaa aaagaagaat gtacaaagag
      301 gtcggttcta taagtacatt tgtacataaa ataaagctta cagaagctat caggaaatta
      361 cgaaggacaa atactcccat ttctgtcata tcatctgagt taggttacag ttcaccatcg
      421 tatttttcta aagtcttctt taaatattta aagacatacc ctcaaaacat aaggaagaaa
      481 aatggtcggt aatgtcacca attaggagga cttgcagagc cgctttttta cgatactgag
      541 atcaaacaga cataatctat gcggagattt acagcagggc atggttcaca aatctgacag
      601 cgatgaatta tcggcactgc gggcagaaaa tgctcg
//
LOCUS       DAZXUK010000625          634 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZXUK010000625 DAZXUK010000000
VERSION     DAZXUK010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.635"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..634
                     /locus_tag="ACH3L8_004613"
                     /pseudo
     CDS             1..634
                     /locus_tag="ACH3L8_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          261 a           98 c           94 g          181 t
ORIGIN      
        1 ctaaatcatt ttaaattgga ttcaacaaat tttactaatg ccaacatgtt tcaggttaat
       61 tttgttagtt caacacaaaa cgccacaatc tcctgggatt atttactaaa aataacgcct
      121 gttttaataa gcattagcga tatgtattct gaagaaaaaa tcaagtttgt cgaaagttgt
      181 ttaaatgagc ttggagacat taccgaagaa caattaaaaa ttatgagatt tgcaattata
      241 aaatctatac caagggcaac tcttacagat aaattagaaa atgaattaac aaaagaaata
      301 tataaaagct catcgaaaat caacaattgc ttgaacagaa ttaaattacc agagatgaaa
      361 gaattctcat caaaaaaaac atatgattac atcgatacaa tcattgaaga ttatgaaaat
      421 atcaaagaaa atgcttatct ggtcgtcccc caaattaatt atactatgga tttaaacata
      481 gaagactcta gctcagaaga gttaataccc tcgagaaaga cgaaaattct ccggacaatg
      541 gctttgaggt cggggaatat aacacatatg aagcatataa ctcagagaat caatatttta
      601 ccagagagga ctatacgtat aattacgacc tttt
//
LOCUS       DAZXUK010000626          624 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZXUK010000626 DAZXUK010000000
VERSION     DAZXUK010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.636"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..251)
                     /locus_tag="ACH3L8_004614"
     CDS             complement(<1..251)
                     /locus_tag="ACH3L8_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFT4087367.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(272..>624)
                     /locus_tag="ACH3L8_004615"
     CDS             complement(272..>624)
                     /locus_tag="ACH3L8_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4087368.1"
                     /translation="TVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRH
                     RKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRR
                     LSESVPCMPHKKAG"
BASE COUNT          141 a          146 c          141 g          196 t
ORIGIN      
        1 ctttgcaggt aagtcagtaa tgtttcttgc tgatcgcgcc agacgttctg cacatcaaca
       61 ttttcattac tgagcagtat tttaactttg ctgacctgca cgccgttgtc gatccagcgt
      121 ttgatctcgc ggatccggtc aatatcggca tcgttgaaca gccgatgacc gccgtctgtc
      181 cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac agggattaaa
      241 ggtaaaccaa ttgattttgc aagtttttat tttaccccgc tttcttatgg ggcatgcatg
      301 ggacactttc agatagtctt ctgttaagaa gttctatctg ttcgtgattg ttgtctttca
      361 tccatgctcc gtaaacattg aataccattt gtgcgtttgt gtggcccatc tggcttgcga
      421 taaaactagg attagctcct gcggcaagtg accagcatgc ataggtatgc ctggattgat
      481 acgattttct gtgtctcaga cctgcgcgtt ttaagatact tgtccatgac tccctgatgg
      541 agtcaacctt atagtgcggt ccggaaaact gccgctgttt tattacctga ggactaaaaa
      601 caaaagtgca tttatgcaca gtac
//
LOCUS       DAZXUK010000627          614 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZXUK010000627 DAZXUK010000000
VERSION     DAZXUK010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.637"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(106..318)
                     /gene="cspH"
                     /locus_tag="ACH3L8_004616"
     CDS             complement(106..318)
                     /gene="cspH"
                     /locus_tag="ACH3L8_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFT4087369.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT          188 a          121 c          127 g          178 t
ORIGIN      
        1 aatggattag tttcttagtg gaaaagtaac tgatgttata atgtatggtg ttgactattg
       61 aactattcca ataaaaccag tcagctttaa acaagcagcg tcatattaag agagataaac
      121 atttgccgct gttggtcctc gcaggccatt tacgcggcaa aattccacgc gtaatcctgg
      181 tataagcact tctgcgtcgc ggggagtgaa tgcggaaata tggacctgga cttctttacg
      241 accgtcggag gggataatga atcctttgcc gcttttgcga tcaaaggttt tgacaattcc
      301 tgtcatttta cgggacaaac aaattcctta ctgaaaatac tgcgctgcac tatacggggt
      361 taataaaata aagccagcga tatttaagac cgccggacgg ctaaaataaa atttgcttaa
      421 tctcaattat catgccttaa tagctgcgtc ggtttgaaag acagacagca tacaaagtag
      481 tttactaaag cagttctcat tatcaggcat tatccccttc ttttgagtct ctctcctgaa
      541 cactaagtgg tttctgtatt aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa
      601 aatatgtcta ataa
//
LOCUS       DAZXUK010000628          607 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZXUK010000628 DAZXUK010000000
VERSION     DAZXUK010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.638"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            39..239
                     /gene="glgS"
                     /locus_tag="ACH3L8_004617"
     CDS             39..239
                     /gene="glgS"
                     /locus_tag="ACH3L8_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFT4087370.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(282..>607)
                     /locus_tag="ACH3L8_004618"
     CDS             complement(282..>607)
                     /locus_tag="ACH3L8_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFT4087371.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
BASE COUNT          168 a          122 c          143 g          174 t
ORIGIN      
        1 tggccttaaa aggtatgcga taaccggagg atgtgcttat ggagcatagt cttaattctt
       61 tgaataattt cgatttcctg gcgcgtagtt ttgccagaat gcacgcagaa ggtcgcccgg
      121 tcgatattct ggctgttact ggtaacatgg atgaagaaca tagaacttgg ttttgcgcac
      181 gttatgcctg gtattgccaa cagatgatgc aggcaagaga gctggagtta gagcactgat
      241 ataacgggcc tgatggcccg ttttagtgtt tgtattagat gctagttata ggtgattttg
      301 aggattacgt ctttactaaa cggtccctca acaacagtct ctgccgggtt cgcactaagt
      361 tctgccctga atggtgcggt gaccgaaggt gcactagacg atgaaaaaga agcaaattgt
      421 gagaagcggt taaattccgt tgctgttcca tctttatgaa atatcttcaa tagcagtcca
      481 ttacccatat ccagatggct ataatcactc aaaatatccg tagtgaaaaa gctccctaaa
      541 atattgaatt gctcagtaca ggccgcacca ggatcttttg tcatgactac actaaacgtc
      601 tcactaa
//
LOCUS       DAZXUK010000629          603 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZXUK010000629 DAZXUK010000000
VERSION     DAZXUK010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.639"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..293
                     /locus_tag="ACH3L8_004619"
     CDS             <1..293
                     /locus_tag="ACH3L8_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFT4087372.1"
                     /translation="AYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLFES
                     LCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            299..>603
                     /locus_tag="ACH3L8_004620"
     CDS             299..>603
                     /locus_tag="ACH3L8_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087373.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVM"
BASE COUNT          173 a          135 c          153 g          142 t
ORIGIN      
        1 gcgcttatcc gatttttgat cgtattgaag aaatggcatg ggctcgtcac taccagcaga
       61 tcgttcgtga agaaaaagaa acggaactgg cggacgacct ggaaaaaggt ctgcctcagc
      121 acctgtttga atcgctctgc atcgaccatt tgcaacgcca cggtgccaat aaacaggcaa
      181 tcagccacgc atttgatgat gatgttgagt ttcaggagcg catggcagaa cacatccggt
      241 acatggctga aaccattgcc caccaccagg ttgatattga tttggaggac taaaatctat
      301 ggcgcttttt caacgagcaa taaacacaca ggcctatcta aaagcaggaa ttatggggtt
      361 tgctggcgac ggcaaaacct acacagcaag cgaactggct attggtctgg taatgcttat
      421 gcggcaacgg ggaattgaag ctggtaaccg cccggtaatg tttctggaca cagaaacagg
      481 ctctgactgg gttaaacccc gctttgatgc ggaaaatatc gaattattta cggcaaaaac
      541 ccgctcgttt gttgatctgc ttgaagccat taatgaagcg gaaagcagcg gttccgtaat
      601 gat
//
LOCUS       DAZXUK010000630          602 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZXUK010000630 DAZXUK010000000
VERSION     DAZXUK010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.640"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            18..>602
                     /locus_tag="ACH3L8_004621"
     CDS             18..>602
                     /locus_tag="ACH3L8_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFT4087374.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITR"
BASE COUNT          130 a          162 c          174 g          136 t
ORIGIN      
        1 gtcgccccgc ccttctgatg ctcccccgtt ttgccgatat ttttcagcag ggaaaccgct
       61 ggcttaactg gctggagaaa caaccggaag gggcagtccg tccggtggta attgaatcag
      121 tgacaaaaat catggcctgc gggaccacac tgatgggcta cacgcagtgg tgttgttcat
      181 caccggactg ttgccataca aaaaaggtct gcttccggtg taaaagccgc tcctgcccgc
      241 actgcggggt gaaggctggc gcacagtgga tacagtatct gctgagcctg gtcccctact
      301 gcccgtggca gcatattgtg ttcacacttc cctgccagta ctggtccctg gtgttccaca
      361 accggtggtt actggcagag atgagccgca ttgcagcgga tgtgatactg gaaatctgcc
      421 atcaggcaga tgtggagccg gggatattca cggtgatcca cacatggggg cgtgaccagc
      481 agtggcatcc gcatatccat ttatcgacaa ctgccggtgg tgtgacgtcg ggccacacct
      541 ggaaaaatct tcatttttac gcccgtaagg tgatgagcat gtggcgctac cggataacac
      601 gg
//
LOCUS       DAZXUK010000631          597 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZXUK010000631 DAZXUK010000000
VERSION     DAZXUK010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.641"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            487..>597
                     /locus_tag="ACH3L8_004622"
     CDS             487..>597
                     /locus_tag="ACH3L8_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087375.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVL"
BASE COUNT          219 a          105 c          122 g          151 t
ORIGIN      
        1 atcccgtact ctctggccag ttgggccacg caggccccta gctggtaaga tttttcaata
       61 agatcaagct taaactccat agagtcatag agtaagaggc acgaatcctg cgggggcgaa
      121 gagaatctga cattgataga gtccattaac gttataagag gactctatca tcgccaatca
      181 tcagacattg aaatagacga cactgagatg acgcttacaa ttcttttggt ttctgtaata
      241 agtaatctga catcccccgt gcccagaaga ggagagggat atcaagccca agggttaata
      301 attgatacta cggttaaaat tgccttcagt aaaggaaacg tgattaaatt caggggattt
      361 cagagtgaaa accatacaac atattaaaat ctgttcattt agctataata catacaagaa
      421 agtgtccagt tagataatgt tatctaaata accacagata aaaacgcaca taattggggg
      481 ggggggatga aaatacctga agcagtaaat catattaatg ttcagaataa tattgatctt
      541 gttgatggaa aaacgaatcc gaataaagca acaaaagcat tacaaaaaaa tgtatta
//
LOCUS       DAZXUK010000632          579 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZXUK010000632 DAZXUK010000000
VERSION     DAZXUK010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.642"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            50..>579
                     /locus_tag="ACH3L8_004623"
     CDS             50..>579
                     /locus_tag="ACH3L8_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087376.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRAD"
BASE COUNT          227 a          120 c           86 g          146 t
ORIGIN      
        1 atccaattta aaaacattag cataaaacag atgatgaata aggaatgtta tgtcaattga
       61 ctttacccca ggtataataa atacatatca cggcgatatt tataactgca caacaaatac
      121 cgataatgtt aaaacgccag acacaccgaa gtggccttgt gataattggg aagaacaaca
      181 acccattaat tccacattct ctggagaagg atacaaccct gaacaatttg atttagcgca
      241 acaacaactc caacaaatta atgcatgcca cacgaacacc acatatacaa atgcagacta
      301 ctcaaaagtt gtggttcaac ttgtaagtct tattaataac attgaaacga taagttcgac
      361 accacttacg caacagacac aatcaatact taatcagatt cataacatta gatacgaaaa
      421 ggataaggat tctgtatgtc aaatcattgt tgtcgttaat ccagaaccca acaatccaat
      481 cataactaaa attctcgtag aagaaggaat acccgaaaga ttcagtgtcc aaaccgtttc
      541 ttccgataat aaaaattttg ctgggcaacg agctgactt
//
LOCUS       DAZXUK010000633          576 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZXUK010000633 DAZXUK010000000
VERSION     DAZXUK010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.643"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            66..427
                     /locus_tag="ACH3L8_004624"
                     /pseudo
     CDS             66..427
                     /locus_tag="ACH3L8_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            514..>576
                     /locus_tag="ACH3L8_004625"
     CDS             514..>576
                     /locus_tag="ACH3L8_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087377.1"
                     /translation="MVTFETVMEIKILHKQGMSSR"
BASE COUNT          151 a          145 c          139 g          141 t
ORIGIN      
        1 ttaaaaatga caatgcctcc aattagttga attaggggtt ataatacccg cttttaggtt
       61 ctttcatggt cacagttaac tttcattgcc cccgttgcca gtccgttcag gtttaccgtc
      121 acgggcagaa cccaaaagga catgatcgat tccgttgccg tgactgtcac cgggtgtttc
      181 agctcactta ctgttacgag gcccgtaaac cgggagttaa aaaccaaatc actgaaatgg
      241 ctttcaacgg tgccggagtt cgcgatactg gccgtacgct gaaaatcggc atcaataccg
      301 tcatacggac attaaaaagc tcccgccccg gcggataaca tctgcgccag tcgctcacgc
      361 tgatgttgcc cttatctgtg aacttgatgg gcagtggagc ttcgttggca gcaaggcgca
      421 acaacactgt cacgaacggt gcaatagtga tccacaccca acgcctgaaa tcagatccag
      481 ggggtaatct gctctcctga ttcaggagag cttatggtca cttttgagac agttatggaa
      541 attaaaatcc tgcacaagca gggaatgagt agccgg
//
LOCUS       DAZXUK010000634          573 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZXUK010000634 DAZXUK010000000
VERSION     DAZXUK010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.644"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>573
                     /gene="rfaY"
                     /locus_tag="ACH3L8_004626"
     CDS             <1..>573
                     /gene="rfaY"
                     /locus_tag="ACH3L8_004626"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFT4087378.1"
                     /translation="DDTKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLI
                     YQTDRVRGEGIQSINDYFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQL
                     SESIKELHQHGMVSGDPHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIK
                     NELKDFGYTYLIFKKKIKKVIRDVKVKLGL"
BASE COUNT          216 a           79 c          102 g          176 t
ORIGIN      
        1 tgatgatact aaagtcattc ttattgacac tgctcgtgga cctcttgtac ttaaagttta
       61 tgcgccaaag cataaaatga ccgaacgttt cttaaaatct tgcataaaaa aagattatta
      121 tgaaaattta atttatcaaa ccgatcgggt acgtggtgaa ggcatccaat caattaatga
      181 ctattttttg ctagctgaac gtaaaacatt aaatttcgct cattattata ttatgctcat
      241 tgagtatatt gaaggtgtcg ggttaaatga gtatttggaa atttctgaag atctgaagga
      301 tcaattatca gaatcgataa aagagttgca tcagcatgga atggtgtctg gcgatcctca
      361 taaaggaaat tttatagtat ccgaaaaagg tttaagacta attgaccttt caggtaaaaa
      421 aacaaccgct gttttaaaag caaaagatcg cattgatctt gaacggcact ataatataaa
      481 aaatgaattg aaagactttg gctatacata tcttatattc aaaaagaaaa ttaaaaaagt
      541 aatcagggat gtaaaagtca aactaggatt gaa
//
LOCUS       DAZXUK010000635          569 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZXUK010000635 DAZXUK010000000
VERSION     DAZXUK010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.645"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>569
                     /gene="virF"
                     /locus_tag="ACH3L8_004627"
     CDS             <1..>569
                     /gene="virF"
                     /locus_tag="ACH3L8_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HFT4087379.1"
                     /translation="SIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRG
                     LNKKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLS
                     FSDQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAK
                     LLLNSQSYINDVSRLIGISSPSYFIRKFN"
BASE COUNT          200 a           85 c           93 g          191 t
ORIGIN      
        1 tctccataaa aaaatctgat agcattaatc catttgagat tataagcctt gacagaaatt
       61 tattattaag cattattaga ataatggaac caatttattc atttcaacac tcctattctg
      121 aggagaaaag ggggttaaac aaaaaaatat tcctcctctc tgaggaggag gtttctatcg
      181 atttgttcaa atctataaaa gagatgcctt tcggcaaaag aaagatctat agtttagctt
      241 gccttttatc agctgtttct gatgaggaag ctttatatac ttcgatatcg atagcttctt
      301 ctcttagttt ttctgatcag ataaggaaga ttgttgaaaa aaacatcgag aagagatggc
      361 gtctttctga tatttcaaat aacttgaatt tatcagaaat agctgttaga aaacgattgg
      421 agagtgaaaa attaacattt caacaaatcc ttcttgatat tcgcatgcat catgcagcaa
      481 agcttttatt gaatagtcaa agctatatta atgatgtatc aagacttatc ggaatatcaa
      541 gcccatctta ttttataagg aaatttaat
//
LOCUS       DAZXUK010000636          560 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZXUK010000636 DAZXUK010000000
VERSION     DAZXUK010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.646"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>560
                     /gene="mxiA"
                     /locus_tag="ACH3L8_004628"
                     /gene_synonym="virH"
     CDS             <1..>560
                     /gene="mxiA"
                     /locus_tag="ACH3L8_004628"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFT4087380.1"
                     /translation="KIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLDQFENRYPDL
                     LKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVITLVEHVRASL
                     SRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDEVMETLAHAL
                     RELRNAKKNFVLLVSVDIRRFVKRLI"
BASE COUNT          192 a           70 c          117 g          181 t
ORIGIN      
        1 aaaattaaaa gtgctcaaga tgaattttat caccagttgt cacaagcttt attaaacaac
       61 ataaatgaga tttttggtat acaagaaaca aaaaatatgt tagatcagtt tgaaaatcgg
      121 tatcctgatc tattaaagga agtcttccga catgtgacta tacagagaat ttctgaggta
      181 ttacaaagat tgcttggaga aaatatttct gttcgcaatt taaaacttat tatggagtct
      241 ttggcgcttt gggctcccag agaaaaagat gtcataacat tagttgaaca tgtccgtgca
      301 tcactttcta ggtatatttg tagtaaaata gctgtttctg gtgagattaa agttgtgatg
      361 ctttccggat atattgagga tgcaataaga aaagggataa ggcaaacctc tggtggctct
      421 ttcttgaata tggatataga ggtttcggat gaggtaatgg aaactttagc acatgctttg
      481 agagaattga gaaatgcaaa aaaaaatttc gttcttttgg tatcagtaga tatacgtagg
      541 tttgttaaaa gacttataga
//
LOCUS       DAZXUK010000637          559 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZXUK010000637 DAZXUK010000000
VERSION     DAZXUK010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.647"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>559
                     /locus_tag="ACH3L8_004629"
     CDS             <1..>559
                     /locus_tag="ACH3L8_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFT4087381.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVSP"
BASE COUNT          165 a          138 c          148 g          108 t
ORIGIN      
        1 tgcgccaact ctgcgtatcc gtgacccgca taaagatgaa aagatggcag caatccataa
       61 agcactggac gaatgcagcg cagagcatcc ggtcttttat gaagatgaag tggatatcca
      121 tcttcatccc aaaatcggtg cggactggca actgcgcgga cagcaaaaac gggtggtcac
      181 gccgggacag aatgaaaaat attatctggc cggagcgctg cacagcggga caggtaaagt
      241 cagctatgtg ggcggcaaca gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct
      301 taaagcgaca taccgtcgtg cgaaaaccat cacgctgatc gtggacaact acattatcca
      361 caaaagccgg gaaacacaga gctggctgaa ggagaacccg aagttcaggg gcatttatca
      421 gccggtttac tcgccatggg tgaatcatgt tgaacggcta tggcaggcac ttcacgacac
      481 aataacgcgt aatcatcagt gccgctcaat gtggcaactg ttgaaaaaag ttcgccattt
      541 tatggaaacc gtcagccca
//
LOCUS       DAZXUK010000638          557 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZXUK010000638 DAZXUK010000000
VERSION     DAZXUK010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.648"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            266..341
                     /locus_tag="ACH3L8_004630"
     tRNA            266..341
                     /locus_tag="ACH3L8_004630"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:299..301,aa:Ile,seq:cat)
     gene            353..>557
                     /locus_tag="ACH3L8_004631"
     CDS             353..>557
                     /locus_tag="ACH3L8_004631"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087382.1"
                     /translation="MYQEELRVQGVLDGGLYFMTNAISRKKCGGWFRMRAGVLVRGTP
                     LQCIYSSVLIQKYLEKMPAMPSGQ"
BASE COUNT          161 a           97 c          132 g          167 t
ORIGIN      
        1 cttcagctaa tgtaataaag ttatattatt agatacaaaa tcattacaaa gtatcattga
       61 atcgatgata actatagata gtgctaatta ttaacatttg cttgttgact gcgagtatcg
      121 actttgcgaa tggaggcggt gtcgggtttt atgtcgcgtg taatcacgac ccaaggtgaa
      181 tgggaacgca taaaaatctg ttatttacat cataagatca tcatgtttat tgcgtggttt
      241 ataagtattg ttattgcata cttaaggccc tttagctcag tggttagagc aggcgactca
      301 taatcgcttg gtcgctggtt caagtccagc aagggccacc aaatcatcat tcatgtatca
      361 ggaagagtta agggtacaag gggtactcga tggcggtcta tatttcatga ctaacgccat
      421 ttcaaggaag aagtgcggtg ggtggtttcg aatgagggct ggtgttttgg tacgtggcac
      481 gccactgcaa tgtatttatt catcagtact cattcaaaag tacctggaaa aaatgcccgc
      541 tatgccaagc gggcaaa
//
LOCUS       DAZXUK010000639          556 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZXUK010000639 DAZXUK010000000
VERSION     DAZXUK010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.649"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..297
                     /gene="pliG"
                     /locus_tag="ACH3L8_004632"
     CDS             <1..297
                     /gene="pliG"
                     /locus_tag="ACH3L8_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFT4087383.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>556)
                     /locus_tag="ACH3L8_004633"
     CDS             complement(405..>556)
                     /locus_tag="ACH3L8_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFT4087384.1"
                     /translation="DPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTTTKML
                     FMCRE"
BASE COUNT          182 a          131 c          102 g          141 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgt
//
LOCUS       DAZXUK010000640          551 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZXUK010000640 DAZXUK010000000
VERSION     DAZXUK010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.650"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>551)
                     /locus_tag="ACH3L8_004634"
     CDS             complement(<1..>551)
                     /locus_tag="ACH3L8_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HFT4087385.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIK"
BASE COUNT          134 a          117 c          135 g          165 t
ORIGIN      
        1 atttaatatc aatctgttga aggtgtatta ttacatcagt agcatattcc agactgcctt
       61 cctggacgat ctgatccata acatcctcac gcactttata tcttgtgcat ttcatttcaa
      121 gtgccgtgag tacagccatt actggtgatg gtagctgtgt tgttttcagg ccgtcgatct
      181 gactgatcgc ttgggcgacg gtgttttgcc attctaaaga acaggtgctg aagtcaaatg
      241 tggctgtttt attatcccgt tgcgacacgc tacaggctaa gcgacgtaat tgggtcaatt
      301 tgtctgcttg ctgaacagga actccgggaa agcggcgatg acttaacgtg gtgtgtacaa
      361 cttcgggcgg aaaaacgaca gcaatatctt tcgccagcca aggtaaaaca gcatctgtat
      421 ttttgtcagc cgcacgggtt tctgctacga aagagggttg ttcctgaata atggtggata
      481 agtccgctac cttgagagag tccgttaccg tttgggaagg ttgcaaagaa ttatctttcg
      541 cttgctccac a
//
LOCUS       DAZXUK010000641          542 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZXUK010000641 DAZXUK010000000
VERSION     DAZXUK010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.651"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          171 a           87 c          101 g          183 t
ORIGIN      
        1 gtcattttat gggaagatta ggagaataaa acagtaatta tatttgagaa atctgctaat
       61 atattcttaa agcttttttg atactgtgaa cgaagtctgt taatggtgga ttttttgttt
      121 atcaccatgt aaaaaaaatt gagtagtaaa agcgttgact gctaatcctc gctgttttta
      181 ttgaaacgat taaaaatacg ctgaatattt tcggccagtc actcatattc acataaaatt
      241 ttgaccatca tgccgcatcc cttagttgca caattcattt tcaatgatat gaatattact
      301 aatttcacca gataattttc tcgtggtcag cagggtatga gttttcaccg taattttaag
      361 gaaacacagt gcgtggggat tgaataacag atagccgacg gaattacccc accggctgaa
      421 agtcaagttc aacgaagatt tttattatct gaattgggca gtttacatca gtgttcgatg
      481 tttatgaaga cgatagatgg cgagaaatta tatcccgaat agcttcatct atttctctct
      541 ga
//
LOCUS       DAZXUK010000642          536 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZXUK010000642 DAZXUK010000000
VERSION     DAZXUK010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.652"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>536
                     /locus_tag="ACH3L8_004635"
     CDS             <1..>536
                     /locus_tag="ACH3L8_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFT4087386.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWL"
BASE COUNT          182 a           82 c          106 g          166 t
ORIGIN      
        1 tatactaaga ttttagagaa tgattttgaa cacccttcaa tacgacgaaa aatagcatcg
       61 ctaagtaatt taggggtaga tgagtggatt cctccggtta tggcattcat gaatcgaatg
      121 gctcgtacag aagattttaa tttagatgat ttttctcaat ttattaccgc ctttgagaag
      181 gtatatatgc atgggtggtt aaaaaaacaa attaaaagcc aaagagaaat ggtttgttat
      241 tctgctctgg tagcaataaa taatgatatg ccttttgatt ctgtgataaa tcagataaac
      301 caacatgcag ataattcagg ttttatagct gctctggatg aagatttata tgagcctcgt
      361 ccgaatcaag tgaacttaat aaaagcaatt ttgcttcgct tagatatgga gcaacaagat
      421 gagagcgtga ttaaaaccta tacggggaga atcacaattg agcatattct tcctcaagca
      481 ttagttaatg aatactggat taatagattc caacctcaag agcatgtcta ctggct
//
LOCUS       DAZXUK010000643          535 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZXUK010000643 DAZXUK010000000
VERSION     DAZXUK010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.653"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>535)
                     /locus_tag="ACH3L8_004636"
     CDS             complement(<1..>535)
                     /locus_tag="ACH3L8_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HFT4087387.1"
                     /translation="ELKNLNALITELKEQNCYVVVVDNRTNFTLKDKLADIEKVHLIC
                     LGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLSCYMDNENAKIAGPV
                     FIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTMVSKEVFEIVGMMDE
                     ELFIDYVDTEWCLRCLNY"
BASE COUNT          166 a          105 c           69 g          195 t
ORIGIN      
        1 catagtttaa gcatctaaga caccattctg tatcgacata atcaataaaa agttcctcat
       61 ccatcattcc aacaatctca aaaacttctt ttgaaaccat ggttccggat gagattgtta
      121 ctgaactttt aaacggtgtc tgtccttcag tgacatgaat tttctctcga agaccatttt
      181 ttttgatatt acaaatagga taataatgtg acttatctct atcaataaaa actgggccag
      241 ctatttttgc attttcatta tccatatagc aggaaagttt tttgataaac tcattgcgaa
      301 tacggctatc ttgatcaaaa aaaattatct tctctgcccc tttttcaaga gaatatcgaa
      361 tcccaatatt ttgtgctttt gctatacctt cattcctacc aagacaaatt aaatgaactt
      421 tttcaatgtc tgctaattta tctttcaagg tgaaattagt cctattatcg actactacaa
      481 cgtaacaatt ttgctccttt agttcagtga tcagtgcatt cagattttta agttc
//
LOCUS       DAZXUK010000644          526 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZXUK010000644 DAZXUK010000000
VERSION     DAZXUK010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.654"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..369)
                     /gene="spa40"
                     /locus_tag="ACH3L8_004637"
                     /gene_synonym="spaS"
     CDS             complement(<1..369)
                     /gene="spa40"
                     /locus_tag="ACH3L8_004637"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HFT4087388.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLK"
     gene            complement(376..>526)
                     /locus_tag="ACH3L8_004638"
     CDS             complement(376..>526)
                     /locus_tag="ACH3L8_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscT/YscT/HrcT family type III secretion system
                     export apparatus protein"
                     /protein_id="HFT4087389.1"
                     /translation="PQMNAFSVSLTIKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFT
                     NLFVR"
BASE COUNT          205 a           77 c           78 g          166 t
ORIGIN      
        1 ttttaaccct ttaacagggt ttaatactga aaaatcaatc ttgatagctt tagtcgcaag
       61 aacaaacttt gtttgaacca atgttggcaa cacagccgaa agaacacaga aaaaaagtgg
      121 gaagccaatt atcttaaaaa agacaataac cactgcaaaa aagtatttac cctcattaat
      181 ttcgaagtca ttaattatta catatctgta taagagcatt acatcactta aggaaaagaa
      241 tgatattata gtaaatgtcc ctaccagaat aataacaaca gtcgttaaat ccttaaattt
      301 aaatgactgt cctttttttg cggcatcctt tagtttttta ggtgtcggct tttctgtttt
      361 atttgccata atattttatc taacaaatag atttgtgaaa aatttatgtt caccgaggaa
      421 aaattgaact ttagaaaagt aaatagtaga agaacagatg aaaataataa atattgcaag
      481 taaactttta atagttaatg atacggaaaa agcattcatc tgcgga
//
LOCUS       DAZXUK010000645          523 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZXUK010000645 DAZXUK010000000
VERSION     DAZXUK010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.655"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>523)
                     /locus_tag="ACH3L8_004639"
     CDS             complement(<1..>523)
                     /locus_tag="ACH3L8_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFT4087390.1"
                     /translation="HIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRDMI
                     LNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRINK
                     NVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGIIR
                     NEENQCYCLGALQV"
BASE COUNT          167 a           94 c           76 g          186 t
ORIGIN      
        1 gaacctgcaa agcaccgaga caataacatt gattttcttc atttcttatt attccgtgat
       61 gactggttaa aactttagtt tctgagttat aattacaaac atcgagcgtt ttaattaagt
      121 tatacgtatc accggcagag agtttataat tttctagtcg tgcagtatag ttaccattaa
      181 aattgatttc atgatttcca gccagaacga tgacattttt attaatacgg ctatctttat
      241 taccctccat atttcgcata gaatttagca aagtaaggat atatttatca ccaaaaatag
      301 tactaaatct atcgcctgta tgatccccaa ggaagataca aggggttatc gcatcgtcct
      361 ttaaaaaaac atgttgatgc aactcattca atatcatgtc tctcgtattt aataaattgt
      421 agagcaattt attattttta tgtatgtatg cagatgaagc catgtcttcg tgttttaata
      481 attcacataa taaactccaa cccttttccc tgatgcctat atg
//
LOCUS       DAZXUK010000646          523 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZXUK010000646 DAZXUK010000000
VERSION     DAZXUK010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.656"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            6..407
                     /locus_tag="ACH3L8_004640"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACH3L8_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          149 a          121 c          138 g          115 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgtccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt ctt
//
LOCUS       DAZXUK010000647          516 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZXUK010000647 DAZXUK010000000
VERSION     DAZXUK010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.657"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..516
                     /locus_tag="ACH3L8_004641"
                     /pseudo
     CDS             1..516
                     /locus_tag="ACH3L8_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816255.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
BASE COUNT          150 a           89 c          113 g          164 t
ORIGIN      
        1 ttcacgacaa aacttagcta ctttctgtca aacatggctt gatgatgaaa tccgtgagtt
       61 gcttaattta gctattgata aaaacatgat ttataaagat gaatatccac aaatggcgga
      121 acctgaaggt cgctgtgtgc gtatgctttc cgattcgtgg agtttccccg attcgcgaca
      181 caccttaggt tgctcaacca ttggttcatc tgaagctgct atgctcggcc gcttggcgtt
      241 aaaatggcag tggtgtaaaa aacgcgaagt acaaggtaaa tcaaccgaac ctgatttgcg
      301 atcctgtgca aatatgctgg cataaattct gccgttactg tgatgtgaaa ttgtgtgaaa
      361 ttccacttga tgatcatcac ctgataatga atgctaaaga ggttttgaag catgtcgatg
      421 aaaacactat tggtgtcgtt acgactttag gtattactta tttttgtaaa gcagattgcg
      481 ttagtgtttt taagataagt gttaaagtta atatta
//
LOCUS       DAZXUK010000648          514 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZXUK010000648 DAZXUK010000000
VERSION     DAZXUK010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.658"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            14..82
                     /gene="ykiE"
                     /locus_tag="ACH3L8_004642"
                     /pseudo
     CDS             14..82
                     /gene="ykiE"
                     /locus_tag="ACH3L8_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
     gene            153..357
                     /locus_tag="ACH3L8_004643"
                     /pseudo
     CDS             153..357
                     /locus_tag="ACH3L8_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            351..455
                     /locus_tag="ACH3L8_004644"
                     /pseudo
     CDS             351..455
                     /locus_tag="ACH3L8_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          156 a          106 c          113 g          139 t
ORIGIN      
        1 aggtaggtat aaaatgggat ggagcctctg cttctgacat gtgtcggtca gaatgactca
       61 tgatgtggtc tgctattatt gacatcctca ctgccctaaa ggatggggat ttcggtaatg
      121 atcttaccca gcaatagtgg acacgcggct aagtgagtaa actctcagtc agaggtgact
      181 cacaagacaa aacagtttca accagtaaaa aaacgtaaac accattcgca tgaatttcgt
      241 agttaagatc tgaagcttgc agaatgcatc ggtgttactg ccggagtccg tgaacttagt
      301 ctgcatgaat caccacagct ttacatctgt cgcagtaaac agcaaaatca gcaaacttac
      361 ttaattatat cgagtgcgat tacaatcgct ggcgatggta cagttggtgt ggcggtctca
      421 gtccggaaca atttgaaaat cataatctca cttaaaacgg catccatttt acaaaagatg
      481 gccgtctgtg tatttgtaat gaataccttc taca
//
LOCUS       DAZXUK010000649          513 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZXUK010000649 DAZXUK010000000
VERSION     DAZXUK010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.659"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>513
                     /gene="lpfB-O113"
                     /locus_tag="ACH3L8_004645"
     CDS             <1..>513
                     /gene="lpfB-O113"
                     /locus_tag="ACH3L8_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFT4087391.1"
                     /translation="EKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRII
                     HTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDA
                     APKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQ
                     MTWRFINDYGA"
BASE COUNT          155 a          120 c          112 g          126 t
ORIGIN      
        1 gaaaaaatac cttatctcat ccaggcttgg gtagatccat ttaataaaga ggataaaagc
       61 aaagcgccgt ttaccgttat tccgccagta tcacgattag aaccttcgca agaaaaaata
      121 ttacgcatta ttcacaccaa aggtgtgtca ttgcctgatg atcgcgagtc agtcttctgg
      181 ctgaatatta aaaatattcc gccatcagcg agcaacaaag cgacgaactc gctggaaata
      241 gcagtaaaaa cgcgtatcaa gctgttctgg cgaccagcaa atatccgcct tattcccgaa
      301 gatgctgcgc cgaaagtgaa atggcgccgg gaagggcgaa atctgatagc agagaatccc
      361 aacccaattc atatcagtgt gatggatgtc atcgttgatg gccatgatgt gcccctcaat
      421 atgatccgtc cttttgaaac gctcacgtta cctttacctg ctaattctgc gggaggccaa
      481 atgacctggc gatttattaa tgattacggc gca
//
LOCUS       DAZXUK010000650          511 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZXUK010000650 DAZXUK010000000
VERSION     DAZXUK010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.660"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..511
                     /locus_tag="ACH3L8_004646"
                     /pseudo
     CDS             1..511
                     /locus_tag="ACH3L8_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAZXUK010000651          509 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZXUK010000651 DAZXUK010000000
VERSION     DAZXUK010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.661"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            189..>509
                     /gene="virB"
                     /locus_tag="ACH3L8_004647"
     CDS             189..>509
                     /gene="virB"
                     /locus_tag="ACH3L8_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFT4087392.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEV"
BASE COUNT          190 a           92 c           84 g          143 t
ORIGIN      
        1 agctctcaca tcagagctcc acaagaatat tattctttta tccaataaag ataaattgca
       61 tcaatccagc tattaaaata gtaattttta agactaccgt tgactatcat caacagttac
      121 atacaatctt gctcacactg catttaactt ttgtcaatat aaatcaatat gaataaacag
      181 ggtgtgatat ggtggatttg tgcaacgact tgttaagtat aaaggaaggc caaaagaaag
      241 agtttacact ccattctggt aataaagttt cctttatcaa agccaagatt cctcataaaa
      301 ggatccaaga tttaaccttc gtcaaccaaa aaacgaatgt acgcgatcaa gaatccctaa
      361 cagaagaatc attagccgat atcataaaaa ctataaagct acaacaattc ttccctgtaa
      421 taggaaggga gattgatggt agaattgaaa ttctggatgg cactcgtaga agagcatctg
      481 caatatatgc aggagcagat cttgaagtt
//
LOCUS       DAZXUK010000652          509 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZXUK010000652 DAZXUK010000000
VERSION     DAZXUK010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.662"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            358..>509
                     /locus_tag="ACH3L8_004648"
     CDS             358..>509
                     /locus_tag="ACH3L8_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFT4087393.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          125 a          135 c          110 g          139 t
ORIGIN      
        1 ctagggtctc tctataggtc ttctctatgg tctctctata ggtctctcta gggtctcctc
       61 taggcattac ccccagaagc taactctatg gccctctatg gtcctctatg gccctgtacg
      121 tcattcttta ggatgccctg tagtttcgta tagcctgatg cctagagaga ccctagagtg
      181 gctcctagag agaccctccc agtagctccg cacagctaca tcgtcatcat catagtattc
      241 aatctcaaag tcattctgtt tgcatcctgt tgtcctgctg gtctcccttc gcttggggac
      301 tttgagtagg tcagtggggg tgcgacttca gggtgcaaac aaaaaggaaa tcaagcgatg
      361 tctcaacaac cacaacaacc acaacaagaa atcagcatca ttaacctcga ccagcttgtg
      421 tccatgacct cagttgaaat tgctgagctg acaggcaagg agcaccgaaa tgtccttcgt
      481 gatattcgta agatgctcga agaacttaa
//
LOCUS       DAZXUK010000653          504 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZXUK010000653 DAZXUK010000000
VERSION     DAZXUK010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.663"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>504
                     /locus_tag="ACH3L8_004649"
     CDS             <1..>504
                     /locus_tag="ACH3L8_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFT4087394.1"
                     /translation="QLSRPSEINRLKALVAKLQRMQFGKSSEKLRAKTERWIQEAQER
                     ISALQEEMAETLGEQYDPVLPSSLRQSSARKPLPASLPRAPRVIRPEEECCPACGGEL
                     SPLGCDVSEQLELISSAFKVIEKQRPKLACRRCDHIVQAPVPSKPIARSYAGAGLLAH
                     VVTGKYAD"
BASE COUNT          123 a          143 c          141 g           97 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggtg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agac
//
LOCUS       DAZXUK010000654          501 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZXUK010000654 DAZXUK010000000
VERSION     DAZXUK010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.664"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..501
                     /locus_tag="ACH3L8_004650"
                     /pseudo
     CDS             1..501
                     /locus_tag="ACH3L8_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
BASE COUNT          114 a          132 c          151 g          104 t
ORIGIN      
        1 tgcagttcac ttacaccgct tctcaacccg gtacgcacca gaaaatcatt gatatggcca
       61 tgaatggcgt tggatgccgg gcaaccgccc gcattatggg cgttggcctc aacacgattt
      121 tccgccattt aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga
      181 cgtcatcgtc tgcgcggaaa tggacgaaca gtggggatac gtcggggcta aatcgcgcca
      241 gcgctggctg ttttacgcgt atgacaggct ccggaagacg gttgttgcgc acgtattcgg
      301 tgaacgcact atggcgacgc tggggcgtct tatgagcctg ctgtcaccct ttgacgtggt
      361 gatatggatg acggatggct ggccgctgta tgaatcccgc ctgaagggaa agctgcacgt
      421 aatcagcaag cgatatacgc agcgaattga gcggcataac ctgaatctga ggcagcacct
      481 ggcacggctg ggacggaagt c
//
LOCUS       DAZXUK010000655          498 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZXUK010000655 DAZXUK010000000
VERSION     DAZXUK010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.665"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          159 a          114 c           84 g          141 t
ORIGIN      
        1 acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttaaac tagccgtctc
       61 aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat ggaacgcagt
      121 aatttcttag tggctattat catgctaatt cttagatccc tagcgattat tcctgcctgg
      181 ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat atgggggaac
      241 gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca ctacgactta
      301 agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc
      361 atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt gaccagttac
      421 aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgagta
      481 aacccttatt ttcaccac
//
LOCUS       DAZXUK010000656          496 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZXUK010000656 DAZXUK010000000
VERSION     DAZXUK010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.666"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          193 a          123 c           64 g          116 t
ORIGIN      
        1 atctccactg gcgagcacgg tcagcacaca aacaaagtta gtcctcctgc ttaatcattg
       61 cgtatcagaa actaaaaaag aaaaaattct gcagacaact taacaatctt catcacattt
      121 cctgaagaca gacatatcca caaacggtag tcagcgttga attaacaagt cttacagagc
      181 tatacgtagc ttataaagcc actcaatgtt catcatcacc cgactactta tctggaatac
      241 caccagtata aactcatcaa taaaaacacc tgttgttaaa ataagaatac tgtcagcagt
      301 aaattattaa aacaacatct gtccacacta tctgttcttt taacgccaac ggcagcgaaa
      361 atagtgcatc acagccttct tttttctttc gccaccactc agacaaacac aacactcaca
      421 cccacaccac aaaacagaat caaaacataa aattaacaat aacagtcaaa aggaaaacac
      481 agaatattga ggtgga
//
LOCUS       DAZXUK010000657          492 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZXUK010000657 DAZXUK010000000
VERSION     DAZXUK010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.667"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>492)
                     /locus_tag="ACH3L8_004651"
     CDS             complement(<1..>492)
                     /locus_tag="ACH3L8_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFT4087395.1"
                     /translation="ANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENID
                     KDAVTNNIALMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKP
                     VHYQDLLTRFSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMS
                     LPV"
BASE COUNT          167 a           98 c           70 g          157 t
ORIGIN      
        1 gaacaggcag agacatgaaa acttcttcat tcaccatcaa gtgaatcatg cattgcgttc
       61 cctttgtcat atccacactt acgctacaaa tatcgtggta atgcgaagag tgcatttcag
      121 gaaacagatt aaacacggta tcttgaaagg cactaaatcg tgttaataaa tcctgataat
      181 gaacaggttt tgtttcaaaa ttatcatcag caaatccaag cactattgca tgctctacat
      241 ctaacgcagt ttcaaatgac tcttcttctg taaaatcatt atttaaaacc tcacctgacg
      301 tattatcact gtcataaaaa gtgctcatca atgcgatatt gtttgttaca gcgtctttat
      361 cgatattctc actaatagca tatcgcctgc tataatcata aaaatgaata aatgcgtcct
      421 ttataaagga tatatattgt tctttgttaa agtcaatacg actgtataac ttttcaaaaa
      481 tcacattcgc aa
//
LOCUS       DAZXUK010000658          490 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZXUK010000658 DAZXUK010000000
VERSION     DAZXUK010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.668"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            96..428
                     /gene="hdeA"
                     /locus_tag="ACH3L8_004652"
     CDS             96..428
                     /gene="hdeA"
                     /locus_tag="ACH3L8_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFT4087396.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
BASE COUNT          154 a           96 c          108 g          132 t
ORIGIN      
        1 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
       61 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
      121 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
      181 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
      241 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
      301 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
      361 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
      421 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
      481 tcttctttaa
//
LOCUS       DAZXUK010000659          485 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZXUK010000659 DAZXUK010000000
VERSION     DAZXUK010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.669"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..485
                     /locus_tag="ACH3L8_004653"
                     /pseudo
     CDS             1..485
                     /locus_tag="ACH3L8_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          140 a          126 c          124 g           95 t
ORIGIN      
        1 tcagggcaaa acaccaaaag cctcccccat taccccggaa caaattgaaa tccgtgagct
       61 caggaaaaag ctacaacgca ttgaaatgga gaatgaaata ttaaaaaagg ctaccgcgct
      121 cttgatgtca gactccctga acagttctcg ataatcggga aactcagagc gcattatcct
      181 gtggtcacac tctgccatgt gttcggggtt catcgcagca gctacagata ctggaaaaac
      241 cgtcctgaaa aaccagacgg cagacgggct gtattacgca gtcaggtact tgagctacat
      301 ggcatcagcc acggttcggc cggagcaaga agcatcgcca caatggcaac ccggagaggc
      361 taccagatgg gacgctggct tgctggcagg ctcatgaaag agctggggct ggtcagctgt
      421 cagcagccga ctcaccggta taaacgtggt ggtcatgaac atgttgctat ccctaactac
      481 cttga
//
LOCUS       DAZXUK010000660          485 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZXUK010000660 DAZXUK010000000
VERSION     DAZXUK010000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.670"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          162 a           80 c           88 g          155 t
ORIGIN      
        1 tttctaagca atgaattgtc attcataaga aactaactga taatgtaaaa aatattcgag
       61 ataaaagttg atcttttaat tttcatgcta ggaacttaac aataatatca ttgttaatgc
      121 atatttatca tatcaaaata ctctgcagaa tgctactatc aaaaccagac aagtgaacaa
      181 aacttggtca acatttagta acgctagttg gtaatgtctt caatcgatag aagtttctgg
      241 taatctactg gtattcaaac agttagggct catacagctt gctggacctt ccccataacc
      301 gcccgaacgt gaaggtctga tgtgttgcaa gagagccgga tgcgggaaat ctgcaagtcc
      361 ggttcgatga gagggacgtt gtttttactt gttgttgatg aagactttca ttattgaaca
      421 gaatgataat attgtctaaa caaaacatta aatctcataa ttctttgtag aaaacgcttt
      481 tcttg
//
LOCUS       DAZXUK010000661          484 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZXUK010000661 DAZXUK010000000
VERSION     DAZXUK010000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.671"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>484
                     /locus_tag="ACH3L8_004654"
     CDS             <1..>484
                     /locus_tag="ACH3L8_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4087397.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     E"
BASE COUNT          109 a          107 c          123 g          145 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaa
//
LOCUS       DAZXUK010000662          482 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZXUK010000662 DAZXUK010000000
VERSION     DAZXUK010000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.672"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(184..273)
                     /gene="tisB"
                     /locus_tag="ACH3L8_004655"
     CDS             complement(184..273)
                     /gene="tisB"
                     /locus_tag="ACH3L8_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFT4087398.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
BASE COUNT          122 a          119 c          134 g          107 t
ORIGIN      
        1 atatacgcca ggaatgggga attgtttagc ggcagaatat gtaaacaaaa gcggcaataa
       61 atgttgccgg gagggatggt agggcggata agatgcgtca gcatcgcatc cgactccaac
      121 ccgcacgcta aatataaaag gggagcggtt acccgctccc ctttggtgcg acttgaatct
      181 gaattacttc aggtatttca gaacagcatc aagcagttgc agtgctgcaa caatgagttt
      241 gaggataaga atggcgatat ccaccaggct catacgcgtc tcctgtggtt caggagcact
      301 gccagctgac gtacctttcc gctgcctgtt gccagctgtt gtgcacgcgg tatctcaagg
      361 agaaacgttg tgcttgttga cataacacag tgtgctcacg cggggttaag gcgctgacgg
      421 caccacccgt ttcagccagg acttagtgcg ccgggtaacg atgcggcccc cgcataacac
      481 at
//
LOCUS       DAZXUK010000663          479 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZXUK010000663 DAZXUK010000000
VERSION     DAZXUK010000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.673"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>479
                     /locus_tag="ACH3L8_004656"
     CDS             <1..>479
                     /locus_tag="ACH3L8_004656"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFT4087399.1"
                     /translation="EYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIASNVKIMGG
                     NHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGNDVVLKG
                     GIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWWNYNYS"
BASE COUNT          148 a           74 c          101 g          156 t
ORIGIN      
        1 gaatattcaa caatgcctgt gaaaaatttc tccagtgtcg gcgctttttc atttccgacc
       61 tgccattttt cagggaatat acgtataggt cgattttgta gtattgcatc aaatgtaaaa
      121 ataatgggtg gaaatcatcc tttgaatcgt tttactactc acatgatgac ttataacggt
      181 gaatttgata agtttgctat gagtgaattt gaacgtagct ggacattgaa accattcatt
      241 accaaacctg aaaatccgat tataggaaat gatgtctgga taggaaatga tgttgtattg
      301 aaaggtggca tcgcaatagg tgatggtgct gttattgctg ccaattccgt tgttaccaaa
      361 gatgtgcccc catatgccat agttgcagga gttcctgcaa aaataatcag atttaggttt
      421 gattcaaatg ttattgatga actgttgaga ataaaatggt ggaattataa ctactcaga
//
LOCUS       DAZXUK010000664          475 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZXUK010000664 DAZXUK010000000
VERSION     DAZXUK010000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.674"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            177..344
                     /gene="rmf"
                     /locus_tag="ACH3L8_004657"
     CDS             177..344
                     /gene="rmf"
                     /locus_tag="ACH3L8_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFT4087400.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
BASE COUNT          127 a          106 c          123 g          119 t
ORIGIN      
        1 gttcctcttc acattttttg tacaaccgac atgcccgtgt agctcacaaa tatgacagtg
       61 gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac tgtgattgca
      121 catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa cgaggcatga
      181 agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag gccggcatcg
      241 ccggacgctc aaaagaaatg tgtccctatc agacgctgag tcaaaggtca caatggctgg
      301 gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt ctctttaaaa
      361 agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatga taatcagaag
      421 gcagacgtat cctggaacag accgactttc aggtcgctgg cggtatagat cagac
//
LOCUS       DAZXUK010000665          470 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZXUK010000665 DAZXUK010000000
VERSION     DAZXUK010000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.675"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          146 a           94 c           99 g          131 t
ORIGIN      
        1 aactgtagat tttcgctcga aaccttcctg tcagctccat agcgaatcaa gcgctgaatg
       61 tcacagtatc gaacagaaaa cagtgacgat ctaacccttc aagaatattc tacgattgtt
      121 ctgtttagga aaagcaaggc gggaagtcgg gagataagtc attgataaaa tggcggagag
      181 agggggattt gaacccccgg tagagttgcc cctactccgg ttttcgagac cgatgataca
      241 gagtcgaaaa atcaaagagt taaaaatttt tcttggaaaa aatgtcttaa aatcgtacac
      301 gaaaaatcaa tgaattacat aacattaaag aagaatattc caagccttct tgagtccacc
      361 acctgttttc aggtgtcatt cagacgtgat ttgccccaga ttgggaaagc acgctttttt
      421 ctcagcttat tccgttttgc ctgtgtgttg aataattcca ctgcagttat
//
LOCUS       DAZXUK010000666          470 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZXUK010000666 DAZXUK010000000
VERSION     DAZXUK010000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.676"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..180
                     /locus_tag="ACH3L8_004658"
     CDS             <1..180
                     /locus_tag="ACH3L8_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFT4087401.1"
                     /translation="DRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEI
                     KHSQPKNRASGKRVS"
BASE COUNT          159 a           97 c           76 g          138 t
ORIGIN      
        1 gacagattta aaaaatattc agcagaagag cagtacgaaa ttgattttca acatcgactg
       61 gtaaacagaa attgttattt agataaatat ggcgatgcac gaatcaatgc ttcttataca
      121 caattagaaa taaaacactc acaacccaaa aatagagcat ccggcaaaag agtgagttaa
      181 catcattgtt aatcaattcg accagtcagt aaatatgctg actggtcgaa cataatttgc
      241 taaaaagtca tcactgactg atgtcagtcc caccatctgc cagttcaatc tacgggtatt
      301 tccagcatat cgcggcacgc gattgctggt ctgttgatgt agtggattgt agttgctgac
      361 ttttcttacc ctcagatttc tgccccactt gtcaattcaa tctgccaata aataacataa
      421 ttatattttt attcctcaca gaaattcctt tttaaaaata gcgaacccac
//
LOCUS       DAZXUK010000667          468 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZXUK010000667 DAZXUK010000000
VERSION     DAZXUK010000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.677"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>468
                     /locus_tag="ACH3L8_004659"
     CDS             <1..>468
                     /locus_tag="ACH3L8_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFT4087402.1"
                     /translation="SGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSI
                     ITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTG
                     NTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASAN"
BASE COUNT          148 a           96 c          103 g          121 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agctaatg
//
LOCUS       DAZXUK010000668          464 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZXUK010000668 DAZXUK010000000
VERSION     DAZXUK010000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.678"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..226
                     /locus_tag="ACH3L8_004660"
     CDS             <1..226
                     /locus_tag="ACH3L8_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFT4087403.1"
                     /translation="SPYLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYG
                     TPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            148..>464
                     /locus_tag="ACH3L8_004661"
     CDS             148..>464
                     /locus_tag="ACH3L8_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFT4087404.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKMTNRRHEE
                     NWRL"
BASE COUNT          156 a           99 c          115 g           94 t
ORIGIN      
        1 atctccttac ctcacaacac gacaagcctg cattgcggct cttcagtctc cgctgcatac
       61 tgtccaggtg agcgcgggtg atggcataac agaggaaaga aaatgtcact cttccgcaga
      121 ggtgaaatat ggtacgccag tttcacattg ccgaacggta aaagatttaa acagtctctt
      181 ggaacaaagg acaaaaggca ggcgacagaa ctccatgaca agctaaaggc tgaagcatgg
      241 cgggtcagca aacttggtga aatacctgat ataacgttcg aggaagcgtg tgtcaggtgg
      301 cttgaagaga aagcacataa aaaatcactg gacgatgaca aaagccggat cggattctgg
      361 cttcaacatt tcgcaggaat gcaactaaga gacattactg aatcaaaaat ttattcagca
      421 atgcagaaaa tgacgaaccg gcgtcatgag gaaaactgga gact
//
LOCUS       DAZXUK010000669          463 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZXUK010000669 DAZXUK010000000
VERSION     DAZXUK010000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.679"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..463
                     /locus_tag="ACH3L8_004662"
                     /pseudo
     CDS             1..463
                     /locus_tag="ACH3L8_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          109 a          133 c          136 g           85 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc aga
//
LOCUS       DAZXUK010000670          462 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZXUK010000670 DAZXUK010000000
VERSION     DAZXUK010000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.680"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..117
                     /locus_tag="ACH3L8_004663"
     CDS             <1..117
                     /locus_tag="ACH3L8_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFT4087405.1"
                     /translation="NGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            104..>462
                     /locus_tag="ACH3L8_004664"
     CDS             104..>462
                     /locus_tag="ACH3L8_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="may be involved in the export or assemby of
                     proteins involved in the entry of Salmonella into host
                     cells; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HFT4087406.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLEN"
BASE COUNT          168 a           57 c           78 g          159 t
ORIGIN      
        1 aatgggtatg catcaacatc ccatttttca aatgagataa aaacacgact aggcttttct
       61 gcaagagaat tatcgaatat tactttttta gtgaaaaaaa taaatgaaaa aatttaatat
      121 taaatctttg actctcttga ttgtattgtt accattgata gttaatgcta ataatattga
      181 ctctcattta ttagaacaaa atgacatagc gaaatatgtt gctcaaagtg atacggtagg
      241 atcttttttt gaacgttttt ctgcgttatt aaattatcct attgttgtta gcaaacaagc
      301 tgcaaaaaag agaatatctg gggagtttga tttatctaat ccagaagaaa tgcttgaaaa
      361 actgacatta cttgtgggac tcatatggta taaagatgga aatgcattat acatatatga
      421 ctctggagaa ttaataagca aagttatttt gctggagaat at
//
LOCUS       DAZXUK010000671          460 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZXUK010000671 DAZXUK010000000
VERSION     DAZXUK010000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.681"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..458)
                     /locus_tag="ACH3L8_004665"
     CDS             complement(<1..458)
                     /locus_tag="ACH3L8_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087407.1"
                     /translation="MYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRNAKTDH
                     SLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGNAKAKV
                     SGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAP"
BASE COUNT          151 a           90 c           78 g          141 t
ORIGIN      
        1 attggagcac atgtgttaat gatcaacccc gggatactaa aaatatagac attattaaac
       61 ttgcttagcc tcgcattgcc tttacctgtt aaagatgcat cgagaaaatt gagtctgaag
      121 ttctcatcct ttatctgatt ttccagtccc gaaactttgg ccttagcatt acctgagatc
      181 cgtccgttac tatctatttt gtaggtaaaa aaatacgaat gggaaaattt cctcggtgta
      241 tcttcatcag ttaagccaac aatcgtaaaa ctaccgctac catctttttt taaaatgact
      301 gaaaggttag cagagtagta taaagaatga tctgttttag catttcttgt taacgtcgac
      361 gtacattcaa gagacgttgt gctaccatca cgagccttcc agagagcaaa aaaacacacc
      421 tgtagcaatg tgaaaacaag tattaatata aaatacattt
//
LOCUS       DAZXUK010000672          460 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZXUK010000672 DAZXUK010000000
VERSION     DAZXUK010000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.682"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(10..291)
                     /locus_tag="ACH3L8_004666"
     CDS             complement(10..291)
                     /locus_tag="ACH3L8_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFT4087408.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          125 a          114 c          112 g          109 t
ORIGIN      
        1 ttctacgtat caggtcaaaa tcattggcca ttgtggcggt gtctgattca tctcttctat
       61 catcaccgac tcgatgttgt gccagatagc ggtgatgtcc gccattgtga tgccaagcaa
      121 ccccattgcg aaccaacagg cggaaacaac gcccagaccg ctgctgatca ccgcaagccc
      181 gacataatca gaccgacgaa aacggatgtt gagtgtgctg gccagaaaca tcattacggc
      241 actaagaagt tgccagcgaa gaagaaccac gccagtggtg agggtagcca tcaaacaatt
      301 cctctgaaaa gagccgatgc cctggacagc gcggcgttgt tcacgggagg cgtggtacac
      361 tctggctatc gcggggcttg cagaacacaa aaatgaaaca ctctgtttgt ttcattaatt
      421 ttgtgaacta tatcacaatt gattgtttgt tagccatatt
//
LOCUS       DAZXUK010000673          459 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZXUK010000673 DAZXUK010000000
VERSION     DAZXUK010000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.683"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>459)
                     /locus_tag="ACH3L8_004667"
     CDS             complement(<1..>459)
                     /locus_tag="ACH3L8_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFT4087409.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          146 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaa
//
LOCUS       DAZXUK010000674          458 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZXUK010000674 DAZXUK010000000
VERSION     DAZXUK010000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.684"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            124..>458
                     /locus_tag="ACH3L8_004668"
     CDS             124..>458
                     /locus_tag="ACH3L8_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087410.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVT"
BASE COUNT          163 a           67 c           93 g          135 t
ORIGIN      
        1 attatgcttt aaggctgaat gatttgtgca aaatataatt atttccccat cgaatatgag
       61 gtgtatgtca taagataaaa tgttaactat gtattcctcg cggtgaataa tatgaggtaa
      121 aagatgaaaa ttggaactgt ggcaggcact aatggcagta caacgacaat cgcaacaaat
      181 gatatggtgc aagagcatgt taccaacttc actaaagaac tgtttgggta tattgcaaat
      241 ggtataggtg atgatatatc aagtattgcc aggacaatgc tcggtgaagt tgtggaaaaa
      301 attgatgatt ggcaaataga acgtttccaa caatctattc aagatgacaa aatttcattt
      361 accatacaaa cgaatcattc agaaaaatat agtatgctat cgggcatgcg cgctcatatt
      421 ctacgtagga ataactgtta tcagtttatt gtgacaat
//
LOCUS       DAZXUK010000675          448 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZXUK010000675 DAZXUK010000000
VERSION     DAZXUK010000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.685"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..382)
                     /locus_tag="ACH3L8_004669"
     CDS             complement(<1..382)
                     /locus_tag="ACH3L8_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087411.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSLL"
BASE COUNT          135 a          106 c           96 g          111 t
ORIGIN      
        1 ttaacagact tgattcatcc tcacaaaata taagtttttc ctggaatgcc catgtctcat
       61 tgaggttcca gatatcaaat tcgaagtcgt gataacgaaa acggatccca ccaaacttat
      121 taaaacgcag ttgtttgacg ggcagttcag ccaatgtctg gaagagttcc tcacgggagc
      181 gaccgatgac gatatcaagg tctgaagaga atccatcgac gccatacaag cctaagtcac
      241 gcaccatccc gccaaaaatc ctggccggac aggaacaggc cagagtatca agtaatactc
      301 tggctttctt atgctcatca ctaagcgtaa aaaaatcggc gatacgctgt tcaagcagtt
      361 gggtgtttga gaccacattc atacagtgat tttagctcat cgtgaaggcg cattaaataa
      421 ctatccgtca tgcctgagat tgcgtcgg
//
LOCUS       DAZXUK010000676          443 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZXUK010000676 DAZXUK010000000
VERSION     DAZXUK010000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.686"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..419
                     /gene="spa40"
                     /locus_tag="ACH3L8_004670"
                     /gene_synonym="spaS"
     CDS             <1..419
                     /gene="spa40"
                     /locus_tag="ACH3L8_004670"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HFT4087412.1"
                     /translation="MKDMMMDKQEIKREYIEQEGHFETKSRRRELHIEILSEQTKSDI
                     RNSKLVVMNPTHIAIGIYFNPEIAPAPFISLIETNQCALAVRKYANEVGIPTVRDVKL
                     ARKLYKTHTKYSFVDFEHLDEVLRLIVWLEQVENTH"
BASE COUNT          157 a           62 c           93 g          131 t
ORIGIN      
        1 acatgaaaga tatgatgatg gataaacagg agataaaaag agaatatata gagcaagagg
       61 gacactttga gacaaagtcg agaaggcgtg agttgcatat cgagattctt tcagagcaga
      121 ctaaatctga tatacgtaat tcaaaattag tggtaatgaa cccgactcat attgcaattg
      181 gtatttattt taatccagaa atagcgcctg caccttttat ttctctcatt gaaactaacc
      241 agtgtgcctt ggctgtcaga aaatatgcaa atgaagttgg tataccgact gtgcgtgatg
      301 tgaaattagc tagaaaacta tataaaacac atacaaaata tagttttgtt gattttgaac
      361 acttggatga agtcctacgt cttattgttt ggcttgagca ggttgaaaac actcattaat
      421 aagtaaagga gatgtgttat atg
//
LOCUS       DAZXUK010000677          443 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZXUK010000677 DAZXUK010000000
VERSION     DAZXUK010000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.687"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>443
                     /gene="ospC1"
                     /locus_tag="ACH3L8_004671"
     CDS             <1..>443
                     /gene="ospC1"
                     /locus_tag="ACH3L8_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFT4087413.1"
                     /translation="EVNKEVSRYVHGSKGIIDVPIFNTKDMKLGLGLYLIDFIRKSED
                     QSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKKVKIREISLEEAVTASN
                     YEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQDVI"
BASE COUNT          166 a           64 c           83 g          130 t
ORIGIN      
        1 cagaggttaa taaagaagtt agtcgatacg tacatggaag taaaggaatt atagatgtac
       61 caatattcaa tactaaagat atgaagttag ggctcggatt atacctgatt gactttatta
      121 gaaaaagtga agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt gcccctgtgg
      181 atctggatag aatcataaac tttgtttttc agccagagta ccatatacct aggatggtaa
      241 gtacagaaaa cttcaaaaaa gttaagatta gagaaatatc cttagaggag gctgttacag
      301 catctaatta cgaagaaatt aacaagcagg tcactaacaa aaaaattgct ctccaggctc
      361 tttttctttc gattactaat caaaaagagg atgtcgcctt atatatatta tctaattttg
      421 agataactag acaagatgtt att
//
LOCUS       DAZXUK010000678          443 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.688, whole genome shotgun sequence.
ACCESSION   DAZXUK010000678 DAZXUK010000000
VERSION     DAZXUK010000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.688"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            213..>443
                     /locus_tag="ACH3L8_004672"
     CDS             213..>443
                     /locus_tag="ACH3L8_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFT4087414.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAZXUK010000679          443 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.689, whole genome shotgun sequence.
ACCESSION   DAZXUK010000679 DAZXUK010000000
VERSION     DAZXUK010000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.689"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            126..386
                     /gene="iraP"
                     /locus_tag="ACH3L8_004673"
     CDS             126..386
                     /gene="iraP"
                     /locus_tag="ACH3L8_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFT4087415.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
BASE COUNT          147 a           82 c           98 g          116 t
ORIGIN      
        1 tggcaatcaa acaaaaaata tttgcgcaaa gtatttcctt tgtcataaaa ataatacttc
       61 cagacactat gaagttgtga aacataatgt taacttctcc atactttgga taaggaaata
      121 cagacatgaa aaatctcatt gctgagttgt tatttaagct tgcccaaaaa gaagaagagt
      181 cgaaagaact gtgtgcgcag gtagaagctt tggagattat cgtcactgca atgcttcgca
      241 atatggcgca aaatgaccaa cagcggttga ttgatcaggt agagggggcg ctgtacgagg
      301 taaagcccga tgccagcatt cctgacgacg atacagagct gctgcgcgat tacgtaaaga
      361 agttattgaa gcatccttgt cagtaaaaag ttaatctttt caacagctgt cataaagttg
      421 tcacggccga gacttatagt cgc
//
LOCUS       DAZXUK010000680          439 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.690, whole genome shotgun sequence.
ACCESSION   DAZXUK010000680 DAZXUK010000000
VERSION     DAZXUK010000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.690"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..202)
                     /locus_tag="ACH3L8_004674"
     CDS             complement(<1..202)
                     /locus_tag="ACH3L8_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFT4087416.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYS"
     gene            302..>439
                     /locus_tag="ACH3L8_004675"
     CDS             302..>439
                     /locus_tag="ACH3L8_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFT4087417.1"
                     /translation="MRMNMSDFATSFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LK"
BASE COUNT          116 a          104 c           92 g          127 t
ORIGIN      
        1 gagaataacg tttatccata ttcttccagt ctggttcatc ttccgacgtc atcaccgggt
       61 tgaaattacg ccgatataaa tcgagttccg tcacctgtat tttccgctcc attgcccgct
      121 gatggatcgc atcagcaata tttgcggtca atgaatcatg acgaggatgg gcccatacca
      181 aataaatacg ttcagactgc ataattttac ctccacgtta agttctggag cgcactttac
      241 gcaggcggca taatcctgta aacgtgatga ttcaggatga gttaatgaag gattttcact
      301 aatgcggatg aatatgtctg actttgccac ttcctttgcc gtggcccgta atcaaagctt
      361 tcgtgcagca ggcgatgagt taggcttatc ctcgtccgcc attagccata gtattaaaac
      421 actggaacaa cgtcttaaa
//
LOCUS       DAZXUK010000681          436 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.691, whole genome shotgun sequence.
ACCESSION   DAZXUK010000681 DAZXUK010000000
VERSION     DAZXUK010000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.691"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          166 a           86 c           75 g          109 t
ORIGIN      
        1 aataaatgtg attctcccgg aactaactaa cctaaccaga cagatgatca ctgtataaaa
       61 acttcagtta atactcattt gttttgtcga ctcagtatat aatgagtgac cagtaatcag
      121 gataatcaca cttacataac ctgtaaaaaa gaacaagaag aataagccac taacataatg
      181 caaaatggca tctgaatcga tcgcagtttg gcattcgaat cgatcatgat ttggcccaat
      241 ccgtgatcga atagagcgga atacgcaaca taattcacca gacaagagct tacaccgaat
      301 tacagattat gtattaatgt caaaactggc atattttaca gatacaatga acatgcaacc
      361 catcaatcta ataggggcgg tatgtacatc tgcccatgaa tgggatggcg ctaagaatac
      421 aaaacaatta gacata
//
LOCUS       DAZXUK010000682          434 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.692, whole genome shotgun sequence.
ACCESSION   DAZXUK010000682 DAZXUK010000000
VERSION     DAZXUK010000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.692"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            35..434
                     /locus_tag="ACH3L8_004676"
                     /pseudo
     CDS             35..434
                     /locus_tag="ACH3L8_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAZXUK010000683          428 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.693, whole genome shotgun sequence.
ACCESSION   DAZXUK010000683 DAZXUK010000000
VERSION     DAZXUK010000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.693"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..132
                     /locus_tag="ACH3L8_004677"
     CDS             <1..132
                     /locus_tag="ACH3L8_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFT4087418.1"
                     /translation="NHAAMNTLLGERVMDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            147..>428
                     /locus_tag="ACH3L8_004678"
     CDS             147..>428
                     /locus_tag="ACH3L8_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFT4087419.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT          105 a           89 c          133 g          101 t
ORIGIN      
        1 aaccatgcag cgatgaatac acttctcggc gagcgggtga tggatcgcat ggtcatgaac
       61 ggcgggcgct gggtgaattt taactgggag agctggcgtc cgaatgttag ccattcgagg
      121 gttgttaagt agtttcagga ggatttatgg cgaaaccttt tactcccgaa cagcgggaag
      181 aactgaagac gcgaattgtg gaactcgtgc atcaggacgg tcgggtcacg attcggcagt
      241 tgtccgatga aacaggtatc agtcgtgcgt ctgtcggtcg cttatgcata gaactggtcg
      301 caagtggtga tgtatataat tctggctacg gcttattccc gtctgaacag gctcgcaagg
      361 actggcaaag cgcccgcaaa aaactctcga gagtaaaggt gaggaaaccg gttgttgttg
      421 atccggac
//
LOCUS       DAZXUK010000684          419 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.694, whole genome shotgun sequence.
ACCESSION   DAZXUK010000684 DAZXUK010000000
VERSION     DAZXUK010000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.694"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..127
                     /locus_tag="ACH3L8_004679"
     CDS             <1..127
                     /locus_tag="ACH3L8_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFT4087420.1"
                     /translation="VSPSQWAAIGVLGSLLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            127..>419
                     /locus_tag="ACH3L8_004680"
     CDS             127..>419
                     /locus_tag="ACH3L8_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFT4087421.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLVTVARQINPYIKVDIPETT"
BASE COUNT          119 a           87 c          109 g          104 t
ORIGIN      
        1 ggtcagtcca tcacagtggg ctgcgattgg tgtgctggga agtctgcttc tggggcttct
       61 gacttatctg acgaatctgt atttcaaaat aagagaagat aagcgtaagg ctgcgagagg
      121 tgaataatgt cgccatcatt acgcaaggct gttgcagttg ctattggtgg cggggctgtt
      181 gctatagcat ctgtgttaat cactggccca ggtggtaacg atggtctgga aggtgtcagc
      241 tacataccat acaaagatat cattggtgta tggactgtat gtcacggaca caccggaaaa
      301 gacatcatgc ctggtaaaac gtataccgaa gcagaatgca aagctctcct gaataaagac
      361 cttgtcactg tcgccagaca aattaacccg tacatcaaag tcgatatacc ggaaacaac
//
LOCUS       DAZXUK010000685          416 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.695, whole genome shotgun sequence.
ACCESSION   DAZXUK010000685 DAZXUK010000000
VERSION     DAZXUK010000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.695"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..373
                     /gene="istB"
                     /locus_tag="ACH3L8_004681"
     CDS             <1..373
                     /gene="istB"
                     /locus_tag="ACH3L8_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFT4087422.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT          121 a           99 c          105 g           91 t
ORIGIN      
        1 aacagcagca gatctgttac ttcagttatc tacggcacaa cgtcagggcc gttataaaac
       61 gacgcttcag cgtggagtaa tggccccccg cctgctcatc attgatgaaa taggctatct
      121 gccgttcagt caggaagaag caaaactgtt cttccaggtc atcgctaaac gttacgaaaa
      181 gagcgcaatg atcctgacat ccaatctacc gttcgggcag tgggatcaaa cgttcgccgg
      241 tgatgcagca ctgacctcag cgatgctggg ccgtatctta caccactcac atgtcgttca
      301 aatcaaagga gaaagctatc gactcagaca gaaacgaaag gccggggtta tagcagaagc
      361 taatcctgag taaaacggtg gatcaatatt gggccgttgg tggagatata agtgga
//
LOCUS       DAZXUK010000686          416 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.696, whole genome shotgun sequence.
ACCESSION   DAZXUK010000686 DAZXUK010000000
VERSION     DAZXUK010000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.696"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..224
                     /locus_tag="ACH3L8_004682"
     CDS             <1..224
                     /locus_tag="ACH3L8_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFT4087423.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..>416
                     /locus_tag="ACH3L8_004683"
     CDS             270..>416
                     /locus_tag="ACH3L8_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFT4087424.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLA"
BASE COUNT          128 a           91 c           99 g           98 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcg
//
LOCUS       DAZXUK010000687          416 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.697, whole genome shotgun sequence.
ACCESSION   DAZXUK010000687 DAZXUK010000000
VERSION     DAZXUK010000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.697"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            43..>416
                     /locus_tag="ACH3L8_004684"
     CDS             43..>416
                     /locus_tag="ACH3L8_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFT4087425.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILN"
BASE COUNT           92 a          111 c          111 g          102 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
      301 cgctgggcgt atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc
      361 tcctcccgtc gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaa
//
LOCUS       DAZXUK010000688          415 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.698, whole genome shotgun sequence.
ACCESSION   DAZXUK010000688 DAZXUK010000000
VERSION     DAZXUK010000688.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.698"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..316)
                     /locus_tag="ACH3L8_004685"
     CDS             complement(<1..316)
                     /locus_tag="ACH3L8_004685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087426.1"
                     /translation="MACDYRYKNRQYHCLSGSYMARSAKPRKRKPAPQRSKLLRYVVK
                     LHEDDFFDEEEAEVLRFDNFDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEI
                     ARC"
BASE COUNT          114 a           94 c           93 g          114 t
ORIGIN      
        1 tgcagcgggc aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt
       61 ttttgtttcc ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat
      121 cgtcaaaatt atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt
      181 gaagttttac gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg
      241 gtttagctga acgggccata taactacctg aaagacaatg atattgtctg tttttataac
      301 ggtaatcaca ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa
      361 cgcttattta gtaccagatc ttacttataa taaaggggct tggcagtttg gataa
//
LOCUS       DAZXUK010000689          412 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.699, whole genome shotgun sequence.
ACCESSION   DAZXUK010000689 DAZXUK010000000
VERSION     DAZXUK010000689.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.699"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          113 a           76 c           71 g          152 t
ORIGIN      
        1 ggggagtctt ttgagcactg gtgttatcaa ttggcttcaa ataattcgcc aataaattat
       61 attgcgatag ctcatcacct tccaggttgt ttatagccag tttatcagcg tcaccaccta
      121 ctgtttatgc ttttatttct ttgattaata agtatatgat attttaattc atctggtgta
      181 tttctttctc atgtgttggt cgactgaata tctgttcagt gaaggggtaa ttttatgttt
      241 gcacttcgaa cattcccttc agttgctagg aaagaaaacc gtaacatttg cgcattaatt
      301 ttaacagtat aatctgagca taaatatgcg ctatatatct ataaaaaggc gcaattaatt
      361 gcgccttttt atcattttat caaaaatggc ccaccgactg tcctcgatag cc
//
LOCUS       DAZXUK010000690          399 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.700, whole genome shotgun sequence.
ACCESSION   DAZXUK010000690 DAZXUK010000000
VERSION     DAZXUK010000690.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.700"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..382
                     /locus_tag="ACH3L8_004686"
     CDS             <1..382
                     /locus_tag="ACH3L8_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087427.1"
                     /translation="IESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRAKEIALDE
                     IIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGV
                     EYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          137 a           91 c           94 g           77 t
ORIGIN      
        1 tatcgagtct cttaacctgg gaggtgaaca ccccggcatc gatacctcac gggataacag
       61 tgaattattt gccaacgacg gtacaccgac atggctaaaa gaaaagcgtg caaaagaaat
      121 cgcacttgat gaaattatcg agttgctcaa caagcaccat gggggcacca gtaacgacgc
      181 caaacgcgca aaagctgatt tgctggaaaa aacattcacc tcccgctcat gggaaagaat
      241 caaaggtatg gactggccga caatcaaagc tggtcgtaat gcactatgga ttgaactgga
      301 aggcgttgaa tatgcattcc cggatccaca aacacaaaac gaaacgcaac aagcaggata
      361 cgatgagaat attccggtgt aaaaccaagt gcattttgg
//
LOCUS       DAZXUK010000691          395 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.701, whole genome shotgun sequence.
ACCESSION   DAZXUK010000691 DAZXUK010000000
VERSION     DAZXUK010000691.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.701"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..290
                     /locus_tag="ACH3L8_004687"
     CDS             <1..290
                     /locus_tag="ACH3L8_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFT4087428.1"
                     /translation="DDHLLHLIRSNAIAPTPVYENYFNNTTAFRPKPEDIFKCGQTLK
                     KTKQKFGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPQ"
BASE COUNT          131 a           71 c           71 g          122 t
ORIGIN      
        1 tggatgatca tcttctccat ctaattagaa gcaatgctat agcacccaca cctgtatatg
       61 aaaattattt taataacact actgctttca gaccaaaacc tgaagatatc ttcaaatgtg
      121 gacagacgct caaaaaaact aaacagaaat ttggccatta tggattaggg gtagaccagc
      181 atcaaaaaat tgataattat accatggggc taaattcttg gaagatagca cctaacgaac
      241 gtggtgataa aaaaggtgtc ccagggctta gttgttttca acctcaataa aacttcatga
      301 gtgctgtggt atctcttttt aattttgcca gtatttcata ggttttctta atcgattgta
      361 gaggtatgta ctgatgacgc ctctacaata tttag
//
LOCUS       DAZXUK010000692          390 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.702, whole genome shotgun sequence.
ACCESSION   DAZXUK010000692 DAZXUK010000000
VERSION     DAZXUK010000692.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.702"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..117
                     /locus_tag="ACH3L8_004688"
                     /pseudo
     CDS             1..117
                     /locus_tag="ACH3L8_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          143 a           52 c           67 g          128 t
ORIGIN      
        1 aagatttctt tttatacata gaaacacagt taaaaattgc aaaagatttt tgtagacctg
       61 gagaagaagt atcaagttct attgcaagta aagtttttca cgcatttatt gatttagtta
      121 acaaaatcag gggtaagaaa gactttatgt atatttgcac gctttgctgt tttgccgagg
      181 aggttaaagg tgattattct cattacagga cctttttatt tgatattggt aatcaatata
      241 aggttaaact tacacaaagc ggaaaaaaga gttctcttta acattagaat ttaacgatac
      301 tataattgaa tctcagaaag tcacaggcaa taaggcaaag catattcttg aggatataga
      361 aatattctat cgtaacaaac ccgatactta
//
LOCUS       DAZXUK010000693          388 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.703, whole genome shotgun sequence.
ACCESSION   DAZXUK010000693 DAZXUK010000000
VERSION     DAZXUK010000693.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.703"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(40..>388)
                     /locus_tag="ACH3L8_004689"
     CDS             complement(40..>388)
                     /locus_tag="ACH3L8_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4087429.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           89 c           87 g          120 t
ORIGIN      
        1 ttcgtgaagc tgttgaaggg tggattctta acggtggcgt taatcagaaa tgctcctgat
       61 gtgccagaac aatttatcag cgtgaagctc atacgctttg cgctgagcct ctatccagtc
      121 gtgtttattg tatacggaca ttatcccgcc aagttcatga ccgagcattc gctcagtaac
      181 atgaggctcg acattcatct cggacagccg tgtaacaagc gttcttctga aatcatgtgt
      241 tcgccagtaa cctagatcca tccctcccct tattctattt acgaatcggt tggctgcagc
      301 gatgctgatc ggtttgttta tgtcttctcc agggaaaaga acatcattgt acattgtcat
      361 ggccttttcg agaaaaggct taatttgt
//
LOCUS       DAZXUK010000694          380 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.704, whole genome shotgun sequence.
ACCESSION   DAZXUK010000694 DAZXUK010000000
VERSION     DAZXUK010000694.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.704"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..248)
                     /locus_tag="ACH3L8_004690"
                     /pseudo
     CDS             complement(1..248)
                     /locus_tag="ACH3L8_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           87 a          121 c           86 g           86 t
ORIGIN      
        1 ctgtctccgt tatggcgtac accacatctt ccagtaatcg ccgcttcacc ggactcagga
       61 ccccgtatca ccatccggaa ccccttatcc gccacatgcc aggagaacct ttccataaac
      121 tcgtcgccac tcatcacaag gttttcttct cgttttgtcc ggtggctgtt gtaacgcagg
      181 ccaatttcat cctgaccggc ataatgttcc aggcggctca tctgcaccgg ctgcttctcc
      241 agccagttaa gccatcggtt tccctgctga aaaatatcgg caaaacgggg aagcatcaga
      301 aaggcggaat aacgccatcc ggccagtgaa ccgtaccgca ttcagggcaa tacatgccgt
      361 cggcgttgat accggcagga
//
LOCUS       DAZXUK010000695          380 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.705, whole genome shotgun sequence.
ACCESSION   DAZXUK010000695 DAZXUK010000000
VERSION     DAZXUK010000695.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.705"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>380
                     /locus_tag="ACH3L8_004691"
     CDS             <1..>380
                     /locus_tag="ACH3L8_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFT4087430.1"
                     /translation="IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRF
                     VNSQAHIIMCGRAGYEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNI
                     ETGQVWRTARILKDRSTRIDGQVF"
BASE COUNT          115 a           84 c          100 g           81 t
ORIGIN      
        1 tcatcgactc catcagtcac ttctggaccg gattatgcga cgagtatgcc agacgccgaa
       61 accgtaaacg aggactggaa ttttcagact gggcgtggct caaacaggag tggcgacgct
      121 ttaccgatcg atttgttaac agtcaggcgc acattattat gtgcgggcgt gcaggatatg
      181 agtatgactt tttcgaaggt gatgatggca aacgccagtt ggcaaaaacc ggtataaaaa
      241 tgaaagcaga aacagaaacc ggttatgaac cctccatccg gatccagatg gagaagcaaa
      301 tgaacataga aaccggtcag gtgtggcgca ccgccagaat actaaaagat cgctccaccc
      361 gtattgatgg acaggtattt
//
LOCUS       DAZXUK010000696          375 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.706, whole genome shotgun sequence.
ACCESSION   DAZXUK010000696 DAZXUK010000000
VERSION     DAZXUK010000696.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.706"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>375
                     /locus_tag="ACH3L8_004692"
     CDS             <1..>375
                     /locus_tag="ACH3L8_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFT4087431.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAZXUK010000697          375 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.707, whole genome shotgun sequence.
ACCESSION   DAZXUK010000697 DAZXUK010000000
VERSION     DAZXUK010000697.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.707"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>375)
                     /gene="mxiG"
                     /locus_tag="ACH3L8_004693"
     CDS             complement(<1..>375)
                     /gene="mxiG"
                     /locus_tag="ACH3L8_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HFT4087432.1"
                     /translation="AETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENN"
BASE COUNT          111 a           69 c           48 g          147 t
ORIGIN      
        1 attattttca caaatctctt tatactggac atttaccttt gtgaaaatac ctttagcatc
       61 cccccttgca ttatgatcgc taagatagtt gaatttaata tttttgatat acggaaattc
      121 aacaatcaga gcatttttca atttatcaag ttcggtatct tttgaactat ttctttcttt
      181 acttaaaaac accagtaatt ccgctttatc actcactaaa ttcaacacat acaaattgat
      241 aaatgggaat cgttgattga tatattgttg aattctattt atttctttgt ttgacaccag
      301 aatatatctg catttggtaa atccggtttt atttaaggct acagacgccc ataccgcatc
      361 attttgtgtt tcggc
//
LOCUS       DAZXUK010000698          374 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.708, whole genome shotgun sequence.
ACCESSION   DAZXUK010000698 DAZXUK010000000
VERSION     DAZXUK010000698.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.708"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>374)
                     /locus_tag="ACH3L8_004694"
     CDS             complement(<1..>374)
                     /locus_tag="ACH3L8_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFT4087433.1"
                     /translation="PDWVSKITHLDQDVLNVLLNGKVKFISEKYNTRYSINYELKDKV
                     DNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYC
                     AKHKFKQKHYMAGIFNYLKYYK"
BASE COUNT          114 a           69 c           57 g          134 t
ORIGIN      
        1 tttataatac tttaaataat taaaaatgcc tgccatataa tgcttttgtt taaatttatg
       61 ttttgcacaa taccgatact gattgctatt tacaggttta agtaaatctt ctttactcca
      121 cggagaagct gctttggcga tcaaaaaact acgtgaaacc ggataatcag cccactcatg
      181 ccagggtttt gtaggtccca catagtgtat aaacacggtg tcatcattga ctggattatc
      241 aactttgtct tttaattcat agttaatact atatcgggta ttatatttct ccgaaataaa
      301 cttcacttta ccattcaata atacattcag tacatcttga tcaaggtggg tgattttact
      361 tacccaatct gggt
//
LOCUS       DAZXUK010000699          372 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.709, whole genome shotgun sequence.
ACCESSION   DAZXUK010000699 DAZXUK010000000
VERSION     DAZXUK010000699.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.709"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..311)
                     /locus_tag="ACH3L8_004695"
     CDS             complement(<1..311)
                     /locus_tag="ACH3L8_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4087434.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAZXUK010000700          365 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.710, whole genome shotgun sequence.
ACCESSION   DAZXUK010000700 DAZXUK010000000
VERSION     DAZXUK010000700.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.710"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>365
                     /locus_tag="ACH3L8_004696"
     CDS             <1..>365
                     /locus_tag="ACH3L8_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFT4087435.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSEN"
BASE COUNT           76 a          124 c          102 g           63 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatg
//
LOCUS       DAZXUK010000701          359 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.711, whole genome shotgun sequence.
ACCESSION   DAZXUK010000701 DAZXUK010000000
VERSION     DAZXUK010000701.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.711"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT           76 a          102 c           90 g           91 t
ORIGIN      
        1 agcctgcgct gatgcagcat gtccctgaag gcgtgattca ggcaccagaa tcacccgacg
       61 ccccggaggc gctgccgggg tgtcgttcca tttctgatga tgacctgtgc gcctggtgta
      121 cccgtctgct ttaccgtccc ggagaaatca gtctgtgcag actgagtata caggcaatgg
      181 catctggcca tccgtctgtg accggaacgg ttacgcgtac ggctgtccgg aatttcagcc
      241 gaatatcatt catccctgat attaccgggt catcacggtc agtgatgaac gtgatgttgc
      301 cagctcccga cctttactcc ctctcattta ttcacattac aaggatttca tacatgaaa
//
LOCUS       DAZXUK010000702          358 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.712, whole genome shotgun sequence.
ACCESSION   DAZXUK010000702 DAZXUK010000000
VERSION     DAZXUK010000702.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.712"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            68..222
                     /locus_tag="ACH3L8_004697"
                     /pseudo
     CDS             68..222
                     /locus_tag="ACH3L8_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611151.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          118 a           80 c           83 g           77 t
ORIGIN      
        1 ataaagtccg gtaatggaga ttgttaatac gatggtgcac gatgcatcgt cagtgcccgg
       61 cccggaggat gccccgtagc tacatacgga tcagggttgg caatatcgaa tggcaggcta
      121 taggcaaagt taaagcccca tggagtagca caaaatatgc cgcacaaagg aaacggtctg
      181 aataacgcag tgatggagaa cttcttcggc acactgcaaa atcgcatcgt ggtcgatcgc
      241 taattcgtcg tgaatcactg aacgatgttc catgcgaaca gatattcgat catatcgaaa
      301 tattaaacat tacccctccc cacattgggc aaaaaaaaac aaaaaacgat caattagt
//
LOCUS       DAZXUK010000703          357 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.713, whole genome shotgun sequence.
ACCESSION   DAZXUK010000703 DAZXUK010000000
VERSION     DAZXUK010000703.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.713"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            39..>357
                     /locus_tag="ACH3L8_004698"
     CDS             39..>357
                     /locus_tag="ACH3L8_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFT4087436.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          112 a           86 c           91 g           68 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gacaggcaga aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc
      301 tggaagatgc atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacc
//
LOCUS       DAZXUK010000704          355 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.714, whole genome shotgun sequence.
ACCESSION   DAZXUK010000704 DAZXUK010000000
VERSION     DAZXUK010000704.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.714"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            119..>355
                     /locus_tag="ACH3L8_004699"
     CDS             119..>355
                     /locus_tag="ACH3L8_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFT4087437.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT          100 a           57 c           93 g          105 t
ORIGIN      
        1 ttaagtcgat tttttatatt ttgtgattag tttcacaaaa taaggtgtta ttcaatgtgt
       61 gctgcactat tcaggggggc tgaaatacag gtggcattta tctgatatgg agaataacat
      121 gaggaaatat attccactgg cattatttat cttttcatgg ccggtattgt ctgcagatat
      181 tcatggacgg gttgttcgtg tactggacgg tgataccatc gaggtcatgg actcactgaa
      241 ggcggtgcgg atcaggctgg taaatattga tgcaccggaa aagaaacagg attatggccg
      301 gtggtcaaca gacatgatga aatcactggt cgcaggaaaa acggttaccg tcaca
//
LOCUS       DAZXUK010000705          353 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.denovo.715, whole genome shotgun sequence.
ACCESSION   DAZXUK010000705 DAZXUK010000000
VERSION     DAZXUK010000705.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.denovo.715"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..264
                     /locus_tag="ACH3L8_004700"
     CDS             <1..264
                     /locus_tag="ACH3L8_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFT4087438.1"
                     /translation="FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISD
                     EDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            288..>353
                     /locus_tag="ACH3L8_004701"
     CDS             288..>353
                     /locus_tag="ACH3L8_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFT4087439.1"
                     /translation="MSVVISGALTDGAGIPMSGYHI"
BASE COUNT           96 a           80 c           90 g           87 t
ORIGIN      
        1 ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat
       61 gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg
      121 ttaatcagcg acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg
      181 caggcgatgg atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc
      241 gcctctccgg aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga
      301 tatcaggtgc gctgactgat ggcgcaggta tccccatgtc cggataccat att
//
LOCUS       DAZXUK010000706         3470 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.guided.324, whole genome shotgun sequence.
ACCESSION   DAZXUK010000706 DAZXUK010000000
VERSION     DAZXUK010000706.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.guided.324"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            60..533
                     /gene="dfrA17"
                     /locus_tag="ACH3L8_004702"
     CDS             60..533
                     /gene="dfrA17"
                     /locus_tag="ACH3L8_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFT4087440.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            664..1452
                     /locus_tag="ACH3L8_004703"
     CDS             664..1452
                     /locus_tag="ACH3L8_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFT4087441.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            1658..2005
                     /locus_tag="ACH3L8_004704"
     CDS             1658..2005
                     /locus_tag="ACH3L8_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFT4087442.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            1999..2838
                     /gene="sul1"
                     /locus_tag="ACH3L8_004705"
     CDS             1999..2838
                     /gene="sul1"
                     /locus_tag="ACH3L8_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFT4087443.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            2966..3169
                     /locus_tag="ACH3L8_004706"
     CDS             2966..3169
                     /locus_tag="ACH3L8_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4087444.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            3325..>3470
                     /locus_tag="ACH3L8_004707"
     CDS             3325..>3470
                     /locus_tag="ACH3L8_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFT4087445.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLS"
BASE COUNT          765 a          920 c          942 g          843 t
ORIGIN      
        1 acgatgttac gcagcagggc agtcgcccta aaacaaagtt agccattaag ggagttaaat
       61 tgaaaatatc attgatttct gcagtgtcag aaaatggcgt aatcggtagt ggtcctgata
      121 tcccgtggtc agtaaaaggt gagcaactac tctttaaagc gctcacatat aatcaatggc
      181 tccttgtcgg aagaaaaaca tttgactcta tgggtgttct tccaaatcgc aaatatgcag
      241 tagtgtcaaa gaacggaatt tcaagctcaa atgaaaacgt cctagttttt ccttcaatag
      301 aaaatgcttt gaaagagcta tcaaaagtta cagatcatgt atatgtctct ggcgggggtc
      361 aaatctataa tagccttatt gaaaaagcag atataattca tttgtctact gttcacgttg
      421 aagtcgaagg tgatatcaaa ttccctataa tgcctgagaa tttcaatttg gtttttgaac
      481 agttttttat gtctaatata aattatacat accagatttg gaaaaaaggc taacaatgcg
      541 ttgcagcacc agtcgcttcg ctccttggac agcttttaag tcgcgtcttt gtggttttgc
      601 tgcgcaaaag tattccacaa agccgcaact taaaagctgc cgctgaactt aacgttaggc
      661 atcatgggtg aatttttccc tgcacaagtt ttcaagcagc tgtcccacgc tcgcgcggtg
      721 atcgagcgcc atctggctgc gacactggac acaatccacc tgttcggatc tgcgatcgat
      781 ggagggctga agccggacag cgacatagac ttgctcgtga ccgtcagcgc cgcacctaac
      841 gattcgctcc ggcaggcgct aatgctcgat ttgctgaaag tctcatcacc gccaggcgat
      901 ggcggaacat ggcgaccgct ggagctaact gttgtcgctc gaagcgaagt agtgccttgg
      961 cgctatccgg cgcggcgtga gcttcagttc ggtgagtggc tccgccacga catcctttcc
     1021 ggaacgttcg agcctgccgt tctggatcac gatcttgcga ttttgctgac caaggcgagg
     1081 caacacagcc ttgcgcttct aggcccatcc gcagccacgt ttttcgagcc ggtgccgaag
     1141 gagcatttct ccaaggcgct tttcgacact attgcccagt ggaatgcaga gtcggattgg
     1201 aagggtgacg agcggaacgt cgttcttgct cttgctcgca tttggtacag cgcttcaact
     1261 ggtctcattg ctcctaagga cgttgctgcc gcatgggtat cggagcgttt gcctgccgag
     1321 catcggcccc tcatctgcaa ggcacgcgcg gcgtacctgg gtagcgagga cgacgaccta
     1381 gcaatgcgcg tcgaagagac ggccgcgttc gttcgatatg ccaaagcaac gattgagaga
     1441 atcttgcgtt gagcggcatg tgcgaaaagt gcatcgaccc gcgccgaggg catctgatgc
     1501 ctaactcggc gttcaagcgg acgggctgcg cccgccgctc aactatgcgt tagatgcact
     1561 aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc
     1621 gtgcataata agccctacac aaattgggag atatatcatg aaaggctggc tttttcttgt
     1681 tatcgcaata gttggcgaag taatcgcaac atccgcatta aaatctagcg agggctttac
     1741 taagcttgcc ccttccgccg ttgtcataat cggttatggc atcgcatttt attttctttc
     1801 tctggttctg aaatccatcc ctgtcggtgt tgcttatgca gtctggtcgg gactcggcgt
     1861 cgtcataatt acagccattg cctggttgct tcatgggcaa aagcttgatg cgtggggctt
     1921 tgtaggtatg gggctcataa ttgctgcctt tttgctcgcc cgatccccat cgtggaagtc
     1981 gctgcggagg ccgacgccat ggtgacggtg ttcggcattc tgaatctcac cgaggactcc
     2041 ttcttcgatg agagccggcg gctagacccc gccggcgctg tcaccgcggc gatcgaaatg
     2101 ctgcgagtcg gatcagacgt cgtggatgtc ggaccggccg ccagccatcc ggacgcgagg
     2161 cctgtatcgc cggccgatga gatcagacgt attgcgccgc tcttagacgc cctgtccgat
     2221 cagatgcacc gtgtttcaat cgacagcttc caaccggaaa cccagcgcta tgcgctcaag
     2281 cgcggcgtgg gctacctgaa cgatatccaa ggatttcctg accctgcgct ctatcccgat
     2341 attgctgagg cggactgcag gctggtggtt atgcactcag cgcagcggga tggcatcgcc
     2401 acccgcaccg gtcaccttcg acccgaagac gcgctcgacg agattgtgcg gttcttcgag
     2461 gcgcgggttt ccgccttgcg acggagcggg gtcgctgccg accggctcat cctcgatccg
     2521 gggatgggat ttttcttgag ccccgcaccg gaaacatcgc tgcacgtgct gtcgaacctt
     2581 caaaagctga agtcggcgtt ggggcttccg ctattggtct cggtgtcgcg gaaatccttc
     2641 ttgggcgcca ccgttggcct tcctgtaaag gatctgggtc cagcgagcct tgcggcggaa
     2701 cttcacgcga tcggcaatgg cgctgactac gtccgcaccc acgcgcctgg agatctgcga
     2761 agcgcaatca ccttctcgga aaccctcgcg aaatttcgca gtcgcgacgc cagagaccga
     2821 gggttagatc atgcctagca ttcaccttcc ggccgcccgc tagcggaccc tggtcaggtt
     2881 ccgcgaaggt gggcgcagac atgctgggct cgtcaggatc aaactgcact atgaggcggc
     2941 ggttcatacc gcgccagggg agcgaatgga cagcgaggag cctccgaacg ttcgggtcgc
     3001 ctgctcgggt gatatcgacg aggttgtgcg gctgatgcac gacgctgcgg cgtggatgtc
     3061 cgccaaggga acgcccgcct gggacgtcgc gcggatcgac cggacattcg cggagacctt
     3121 cgtcctgaga tccgagctcc tagggatcgc ctcagaaaac ggaaaataaa gcacgctaag
     3181 ccgtaagtaa gcgtgctcct gtgaaagcca cagctaaaac tgcgtagtac acatagaggt
     3241 atttttttct agatttatat cgaatttcgt tatcgaattt cgcttattat tgatcaactc
     3301 gttgcagatc atgaaggcga aagtatgacg aataacccta ccgatgacag cagaccatgg
     3361 tcggtctttc ttatttttct gcggcttgga ttgacatctt ttggcggccc cattgcgcac
     3421 ttgggctact tccgcgccga atttgtcaca cggcggcgct ggctctccga
//
LOCUS       DAZXUK010000707         3393 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.guided.331, whole genome shotgun sequence.
ACCESSION   DAZXUK010000707 DAZXUK010000000
VERSION     DAZXUK010000707.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.guided.331"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            110..1015
                     /locus_tag="ACH3L8_004708"
     CDS             110..1015
                     /locus_tag="ACH3L8_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFT4087446.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1012..2250
                     /gene="mrx(A)"
                     /locus_tag="ACH3L8_004709"
                     /gene_synonym="mrx"
     CDS             1012..2250
                     /gene="mrx(A)"
                     /locus_tag="ACH3L8_004709"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFT4087447.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2250..2834
                     /gene="mphR(A)"
                     /locus_tag="ACH3L8_004710"
     CDS             2250..2834
                     /gene="mphR(A)"
                     /locus_tag="ACH3L8_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFT4087448.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
BASE COUNT          563 a         1046 c         1091 g          693 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttccg gatcggcaac
     1081 gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc atacaaagag cgcggcctgg
     1141 gcgggcgcca cggccgctag cagcgtcatc gcgaccatca tcggcgcgtg ggttggtggt
     1201 ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga tctcgggtgt ggtgggcggc
     1261 gtggccatgg cgagcatccc actgctcgat gccgttggcg ccctctcgaa cactgggctg
     1321 atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg gtatggccgc gcaggacagt
     1381 gagctgccca aactcggcca cgtcgccggg ctctccgttg agcgcgtctc gtcactgaaa
     1441 gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc ttgggggggc cgcaatcggc
     1501 ctgcttggcg ctgcgccaac gctcgggctg acggcgttct gctccgtcct tgcaggtctg
     1561 ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga tgaccacgac ggcgactctc
     1621 tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc tgctgcgccc tctctttggt
     1681 atagtgatga tcttcgtggg catcgttggc gccaacggca gcgtcatcat gcctgcgctg
     1741 tttgtagatg caggacgcca agtagcagag ctcgggctgt tctcctcaat gatgggggct
     1801 ggtggtctcc ttggcattgc cattcatgcg tcggtcggcg cccggatatc agcgcagaac
     1861 tggctggcgg tggcattttg tggctctgcg gtgggctcgc ttctgctttc acagttgcca
     1921 ggcgtgccgg tgctgatgtt gttgggcgcg ctcgtgggac tgctgaccgg ctcagtctct
     1981 cccattctca acgctgccat ctacaaccgc acgccgccag aacttctcgg ccgggtactc
     2041 ggcacggtct cggcggtgat gctgtcagcc tcgcccatgg ttatgcttgc ggccggcgcg
     2101 tttgtcgacc ttgctggtcc gctccctggc ctcgttgtat cggccgtgtt tgcggggctc
     2161 gtggctctac tctcgctccg tcttcaattt gctacaatgg cggcggcagc cacagcctcc
     2221 gccccaaccc atacagaagg tgaacactga tgccccgccc caagctcaag tccgatgacg
     2281 aggtactcga ggccgccacc gtagtgctga agcgttgcgg tcccatagag ttcacgctca
     2341 gcggagtagc aaaggaggtg gggctctccc gcgcagcgtt aatccagcgc ttcaccaacc
     2401 gcgatacgct gctggtgagg atgatggagc gcggcgtcga gcaggtgcgg cattacctga
     2461 atgcgatacc gataggcgca gggccgcaag ggctctggga atttttgcag gtgctcgttc
     2521 ggagcatgaa cactcgcaac gacttctcgg tgaactatct catctcctgg tacgagctcc
     2581 aggtgccgga gctacgcacg cttgcgatcc agcggaaccg cgcggtggtg gaggggatcc
     2641 gcaagcgact gcccccaggt gctcctgcgg cagctgagtt gctcctgcac tcggtcatcg
     2701 ctggcgcgac gatgcagtgg gccgtcgatc cggatggtga gctagctgat catgtgctgg
     2761 ctcagatcgc tgccatcctg tgtttaatgt ttcccgaaca cgacgatttc caactcctcc
     2821 aggcacatgc gtaaacggag gtgtgcagag tccctgcggc aggcgacgaa cacgaccgtc
     2881 gtcgattagt accggtacgg tcggtggtat cgaagtcttg atcaccactc aggtctacgg
     2941 cttacaaatg gtgaccatcc cgatacttgc gtcagagcac cgggccgatt ctttgacagt
     3001 gaatcactcc cgtaaggttg tgccggtgtg ggtgtcccgg gtcgagacga tactccgcca
     3061 atgcgcccag caaacaacct ggccatcgca ggtggtgggg agcggtgtgg cggatgagtt
     3121 ggacaagttg gtgtagcagc acgagcacgg cgagataaca tcgcaggagt tcgacatgct
     3181 caagagacag ctgattgcga atcgcgatgc agattcataa cccgattgcg ggttggcttc
     3241 actccaccat caccgagcag actagcacgg cgggctctgt tgcaaagatt ggcggcagtc
     3301 agaggtaggc tgtcgctctg cgccgatcag gcggctgctg cgaaatggtg gttgagcatg
     3361 cccatggcct ccgtcagcgc cgagggccca atg
//
LOCUS       DAZXUK010000708         2564 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.guided.387, whole genome shotgun sequence.
ACCESSION   DAZXUK010000708 DAZXUK010000000
VERSION     DAZXUK010000708.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.guided.387"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..145
                     /locus_tag="ACH3L8_004711"
     CDS             <1..145
                     /locus_tag="ACH3L8_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
                     /protein_id="HFT4087449.1"
                     /translation="SQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDFLIATL
                     KPR"
     gene            156..551
                     /gene="frdC"
                     /locus_tag="ACH3L8_004712"
     CDS             156..551
                     /gene="frdC"
                     /locus_tag="ACH3L8_004712"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFT4087450.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            562..921
                     /gene="frdD"
                     /locus_tag="ACH3L8_004713"
     CDS             562..921
                     /gene="frdD"
                     /locus_tag="ACH3L8_004713"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFT4087451.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            984..2117
                     /locus_tag="ACH3L8_004714"
     CDS             984..2117
                     /locus_tag="ACH3L8_004714"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFT4087452.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            2206..>2564
                     /gene="blc"
                     /locus_tag="ACH3L8_004715"
     CDS             2206..>2564
                     /gene="blc"
                     /locus_tag="ACH3L8_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFT4087453.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIA"
BASE COUNT          621 a          650 c          659 g          634 t
ORIGIN      
        1 cagccagaac ggtgtatgga gctgtacttt cgtgggctat tgctccgaag tctgcccgaa
       61 acacgtcgat ccggctgcgg ccattcagca gggcaaagta gaaagttcga aagactttct
      121 tatcgcgacc ctgaaaccac gctaaggagt gcaacatgac gactaaacgt aaaccgtatg
      181 tacggccaat gacgtccacc tggtggaaaa aattgccgtt ttatcgcttt tacatgctgc
      241 gcgaaggcac ggcggttccg gctgtgtggt tcagcattga actgattttc gcgctgtttg
      301 ccctgaaaaa tggcccggaa gcctgggcgg gattcgtcga ctttttacaa aacccggtta
      361 tcgtgatcat taacctgatc actctgacgg cagctctgct gcacaccaaa acctggtttg
      421 aactggcacc gaaagcggcc aatatcattg taaaagacga aaaaatggga ccagagccaa
      481 ttatcaaaag tctctgggcg gtaactgtgg ttgccaccat cgtaatcctg tttgttgccc
      541 tgtactggta aggagcctga gatgattaat ccaaatccaa agcgttctga cgaaccggta
      601 ttctggggcc tcttcggggc cggtggtatg tggagcgcaa ttattgcgcc ggtgatgatc
      661 ctgctggtgg gtattctgct gccactgggg ctgtttccgg gtgatgcgct gagctacgag
      721 cgcgttctgg cgttcgcgca gagcttcatt ggtcgcgtat tcctgttcct gatgatcgtt
      781 ctgccgctgt ggtgtggttt acaccgtatg caccacgcga tgcacgatct gaaaatccac
      841 gtgcctgcgg gcaaatgggt tttctacggt ctggctgcta tcctgacagt tgtcacgctg
      901 attggtgtcg ttacaatcta acgcatcgcc aatgtaaatc cggcccgcct atggcgggcc
      961 gttttgtatg gaaaccagac cttatgttca aaacgacgct ctgcgcctta ttaattaccg
     1021 cctcttactc cacatttgct gcccctcaac aaatcaacga tattgtgcat cgcacaatta
     1081 ccccgcttat agagcaacaa aagatccccg gtatggcggt ggcggtaatt tatcagggta
     1141 aaccttatta ctttacctgg ggctatgcgg acatcgccaa aaagcagccc gtcacacagc
     1201 aaacgttgtt tgagttaggt tcggtcagca aaacatttac gggcgtgctt ggtggcgacg
     1261 ctattgctcg aggggaaatc aagttaagcg atcccgcaac aaaatactgg cctgaactta
     1321 ccgctaaaca gtggaatggg atcacactat tacatcttgc gacctacacc gctggcggcc
     1381 tgccattgca ggtgccggat gaagtgaaat cctcaagcga cttgctgcgc ttctatcaaa
     1441 actggcagcc tgcatgggct ccaggaacac aacgtctgta tgccaactcc agtatcggtt
     1501 tgttcggcgc actggctgtg aagccgtctg gtttgagttt tgagcaggcg atgcaaactc
     1561 gtgtcttcca gccactcaaa ctcaaccata cgtggattaa tgtacctccc ccagaagaaa
     1621 agaattacgc ctggggatat cgcgaaggta aggcagtgca tgtttcgcca ggggcgttag
     1681 atgctgaagc ttatggtgtg aagtcgacca ttgaagatat ggcccgctgg gtgcaaagca
     1741 atttaccccc ccttgatatc aatgagaaaa cgcttcaaca agggatacaa ctggcacaat
     1801 ctcgctactg gcaaaccggc gatatgtatc agggcctggg ctgggaaatg ctggactggc
     1861 cggtcaatcc tgacagcatc attaacggca gtgacaataa aattgcactg gcagcacgcc
     1921 cggtaaaagc gattaagccc ccaactcctg cagtacgcgc atcatgggta cataaaacgg
     1981 gggcgaccgg cggatttggt agctatgtcg cgtttattcc agaaaaagag ctgggtatcg
     2041 tgatgctggc taacaaaaac tatcccaatc cagcgagagt cgccgccgcc tgtcagattc
     2101 tcaacgctct acagtaaaat ttcatcgggt ccgaattttc ggaccttttc tgcgcttttc
     2161 cttgctgtca tctacactta gaaaaaaacc agtaaggaaa catttatgcg cctgctccct
     2221 ctcgttgccg cagcgacagc tgcatttctg gtcgttgcct gtagttttcc tacgccgccg
     2281 cgtggcgtga ccgtagtgaa taatttcgac gccaaacgtt atctcggtac ctggtatgag
     2341 attgcccgtt ttgatcaccg ttttgaacgt ggactggaaa aagtcaccgc aacatacagc
     2401 ctgcgtgatg acggcggcct gaatgtcatt aataaaggct ataaccctga cagaggaatg
     2461 tggcagcaga gtgaagggaa agcgtacttt accggcgcac caactcgcgc tgcgctgaaa
     2521 gtgtcattct ttggtccttt ctatggcggt tataacgtta ttgc
//
LOCUS       DAZXUK010000709         1638 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.guided.461, whole genome shotgun sequence.
ACCESSION   DAZXUK010000709 DAZXUK010000000
VERSION     DAZXUK010000709.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.guided.461"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            37..1242
                     /gene="tet(B)"
                     /locus_tag="ACH3L8_004716"
     CDS             37..1242
                     /gene="tet(B)"
                     /locus_tag="ACH3L8_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFT4087454.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1355..>1638)
                     /locus_tag="ACH3L8_004717"
     CDS             complement(1355..>1638)
                     /locus_tag="ACH3L8_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFT4087455.1"
                     /translation="KNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLK
                     AYDDDVLKNLLNGWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          405 a          303 c          368 g          562 t
ORIGIN      
        1 ttttaccact ccctatcagt gatagagaaa agtgaaatga atagttcgac aaagatcgca
       61 ttggtaatta cgttactcga tgccatgggg attggcctta tcatgccagt cttgccaacg
      121 ttattacgtg aatttattgc ttcggaagat atcgctaacc actttggcgt attgcttgca
      181 ctttatgcgt taatgcaggt tatctttgct ccttggcttg gaaaaatgtc tgaccgattt
      241 ggtcggcgcc cagtgctgtt gttgtcatta ataggcgcat cgctggatta cttattgctg
      301 gctttttcaa gtgcgctttg gatgctgtat ttaggccgtt tgctttcagg gatcacagga
      361 gctactgggg ctgtcgcggc atcggtcatt gccgatacca cctcagcttc tcaacgcgtg
      421 aagtggttcg gttggttagg ggcaagtttt gggcttggtt taatagcggg gcctattatt
      481 ggtggttttg caggagagat ttcaccgcat agtccctttt ttatcgctgc gttgctaaat
      541 attgtcgctt tccttgtggt tatgttttgg ttccgtgaaa ccaaaaatac acgtgataat
      601 acagataccg aagtaggggt tgagacgcaa tcgaattcgg tatacatcac tttatttaaa
      661 acgatgccca ttttgttgat tatttatttt tcagcgcaat tgataggcca aattcccgca
      721 acggtgtggg tgctatttac cgaaaatcgt tttggatgga atagcatgat ggttggcttt
      781 tcattagcgg gtcttggtct tttacactca gtattccaag cctttgtggc aggaagaata
      841 gccactaaat ggggcgaaaa aacggcagta ctgctcggat ttattgcaga tagtagtgca
      901 tttgcctttt tagcgtttat atctgaaggt tggttagttt tccctgtttt aattttattg
      961 gctggtggtg ggatcgcttt acctgcatta cagggagtga tgtctatcca aacaaagagt
     1021 catcagcaag gtgctttaca gggattattg gtgagcctta ccaatgcaac cggtgttatt
     1081 ggcccattac tgtttgctgt tatttataat cattcactac caatttggga tggctggatt
     1141 tggattattg gtttagcgtt ttactgtatt attatcctgc tatcgatgac cttcatgtta
     1201 acccctcaag ctcaggggag taaacaggag acaagtgctt agttatttcg tcaccaaatg
     1261 atgttattcc gcgaaatata atgaccctct tgataaccca agagggcatt ttttacgata
     1321 aagaagattt agcttcaaat aaaacctatc tattttattt atctttcaag ctcaataaaa
     1381 agccgcggta aatagcaata aattggcctt ttttatcggc aagctctttt aggtttttcg
     1441 catgtattgc gatatgcata aaccagccat tgagtaagtt tttaagcaca tcatcatcat
     1501 aagctttaag ttggttctct tggatcaatt tgctgacaat ggcgtttacc ttaccagtaa
     1561 tgtattcaag gctaattttt tcaagttcat tccaaccaat gataggcatc acttcttgga
     1621 tagggataag gtttttat
//
LOCUS       DAZXUK010000710         1217 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.guided.517, whole genome shotgun sequence.
ACCESSION   DAZXUK010000710 DAZXUK010000000
VERSION     DAZXUK010000710.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.guided.517"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..236
                     /locus_tag="ACH3L8_004718"
     CDS             <1..236
                     /locus_tag="ACH3L8_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFT4087456.1"
                     /translation="NMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKY
                     VFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            349..1187
                     /gene="aadA1"
                     /locus_tag="ACH3L8_004719"
                     /pseudo
     CDS             349..1187
                     /gene="aadA1"
                     /locus_tag="ACH3L8_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          340 a          289 c          307 g          281 t
ORIGIN      
        1 agaacatgta tctacaagat ctggataata gtacaaaact gtatgggaaa actggtgcag
       61 gattcacagc aaatagaacc ttacaaaacg gatggtttga agggtttatt ataagcaaat
      121 caggacataa atatgttttt gtgtccgcac ttacaggaaa cttggggtcg aatttaacat
      181 caagcataaa agccaagaaa aatgcgatca ccattctaaa cacactaaat ttataaaaaa
      241 tctaatggca aaatcgccca acccttcaat caagtcggga cggccaaaag caagcttttg
      301 gctcccctcg ctggcgctcg gcgcccctta tttcaaacgt taaacatcat gagggaagtg
      361 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
      421 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
      481 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
      541 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
      601 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
      661 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
      721 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
      781 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
      841 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
      901 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
      961 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     1021 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     1081 cagttggaag aatttgttca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     1141 tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
     1201 tgcactaagc acataat
//
LOCUS       DAZXUK010000711         1135 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.guided.532, whole genome shotgun sequence.
ACCESSION   DAZXUK010000711 DAZXUK010000000
VERSION     DAZXUK010000711.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.guided.532"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            38..898
                     /locus_tag="ACH3L8_004720"
     CDS             38..898
                     /locus_tag="ACH3L8_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFT4087457.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          305 a          250 c          281 g          299 t
ORIGIN      
        1 ctggtaaatg cttcaataat attgaaaaag gaagagtatg agtattcaac attttcgtgt
       61 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      121 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      181 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      241 cacttttaaa gttctgctat gtggtgcggt attatcccgt gttgacgccg ggcaagagca
      301 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
      361 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
      421 tgataacact gctgccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
      481 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
      541 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
      601 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
      661 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
      721 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
      781 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
      841 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
      901 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
      961 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1021 ttcgttccac tgagcgtcag accccttatg gcagagcagg gaaaggaatt gccgggctat
     1081 gtgcaacggg aatttgaaga atttctccaa tgcgggcggc tggagcatgg ctttc
//
LOCUS       DAZXUK010000712         1129 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.guided.533, whole genome shotgun sequence.
ACCESSION   DAZXUK010000712 DAZXUK010000000
VERSION     DAZXUK010000712.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.guided.533"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            130..867
                     /gene="erm(B)"
                     /locus_tag="ACH3L8_004721"
     CDS             130..867
                     /gene="erm(B)"
                     /locus_tag="ACH3L8_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFT4087458.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          422 a          187 c          180 g          340 t
ORIGIN      
        1 ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta aacagataac
       61 taaaattaca aacaaatcgt ttaacttctg tatttgttta tagatgtaat cacttcagga
      121 gtaaattaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag tgaaaaagta
      181 ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta cgaaattgga
      241 acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt aacgtctatt
      301 gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa tactcgtgtc
      361 actttaattc accaagatat tctacagttt caattcccta acaaacagag gtataaaatt
      421 gttgggaata ttccttacca tttaagcaca caaattatta aaaaagtggt ttttgaaagc
      481 catgcgtctg acatctatct gattgttgaa gaaggattct acaagcgtac cttggatatt
      541 caccgaacac tagggttgct cttgcacact caagtctcga ttcagcaatt gcttaagctg
      601 ccagcggaat gctttcatcc taaaccaaga gtaaacagtg tcttaataaa acttacccgc
      661 cataccacag atgttccaga taaatattgg aagctatata cgtactttgt ttcaaaatgg
      721 gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc aatgaaacac
      781 gccaaagtaa acaatttaag taccgttact tatgagcaag tattgtctat ttttaatagt
      841 tatctattat ttaacgggag gaaataattc tatgagtcgc ttttgtaaat ttggaaagtt
      901 acacgttact aaagggaatg tagataaatt attaggtata ctactgacag cttccaagga
      961 gctaaagagg tccctagact agcaagaagt acacaagaag ccttaaagat tatagaaaag
     1021 cttttccatg aaatgcttaa cattatttaa tgttaacatg tggctttgtg gtaactacca
     1081 cgagtggtaa cttgtaacat gaaaaaagtt acaagttaca agtggttta
//
LOCUS       DAZXUK010000713         1052 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.guided.546, whole genome shotgun sequence.
ACCESSION   DAZXUK010000713 DAZXUK010000000
VERSION     DAZXUK010000713.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.guided.546"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            264..923
                     /locus_tag="ACH3L8_004722"
     CDS             264..923
                     /locus_tag="ACH3L8_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFT4087459.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT          291 a          227 c          245 g          289 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg tt
//
LOCUS       DAZXUK010000714          969 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.guided.557, whole genome shotgun sequence.
ACCESSION   DAZXUK010000714 DAZXUK010000000
VERSION     DAZXUK010000714.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.guided.557"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..164)
                     /locus_tag="ACH3L8_004723"
     CDS             complement(<1..164)
                     /locus_tag="ACH3L8_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFT4087460.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGN"
     gene            426..884
                     /gene="gpt"
                     /locus_tag="ACH3L8_004724"
     CDS             426..884
                     /gene="gpt"
                     /locus_tag="ACH3L8_004724"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFT4087461.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
BASE COUNT          247 a          227 c          242 g          253 t
ORIGIN      
        1 atattaccta cctgatcgcg ttcgacatgg aaacctttct cttttgccca accaacaatg
       61 tattcagcga gttgctcttc atgataggac gggtgaggaa tagaacagat tttggcaaaa
      121 atatcccaca gcggctgtgg agataattga gacagttcag acacgttaag tctccttgtc
      181 gatcacccgc aaaacagtat tgcaggtcac agggttagca gaaaatgttg tcaacacaag
      241 acaggcttgc gagatatgta ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
      301 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
      361 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
      421 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
      481 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
      541 gtctggtacc gggggcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
      601 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag
      661 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgctgttg
      721 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
      781 gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatc gaacagccgt
      841 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
      901 cattgccggg cgttgttctt tttaacttca ggcaggttac aatagtttcc agtaagtatt
      961 ctggaggct
//
LOCUS       DAZXUK010000715          721 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF09
            SAMN39618780-rid23473033.guided.612, whole genome shotgun sequence.
ACCESSION   DAZXUK010000715 DAZXUK010000000
VERSION     DAZXUK010000715.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618780
            Sequence Read Archive: SRR27734391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394703.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394703.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:45:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,725
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 226 of 478
            Pseudo Genes (incomplete)         :: 160 of 478
            Pseudo Genes (internal stop)      :: 167 of 478
            Pseudo Genes (multiple problems)  :: 68 of 478
            Pseudo Genes (short protein)      :: 1 of 478
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618780-rid23473033.guided.612"
                     /isolate="16-00PHL-SF09"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            109..>721
                     /locus_tag="ACH3L8_004725"
     CDS             109..>721
                     /locus_tag="ACH3L8_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFT4087462.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQ"
BASE COUNT          274 a          141 c          118 g          188 t
ORIGIN      
        1 acgatgttac gcagcagggc agtcgcccta aaacaaagtt gggcgaaccc ggagcctcat
       61 taattgttag ccgttaaaat taagcccttt accaaaccaa tacttattat gaaaaacaca
      121 atacatatca acttcgctat ttttttaata attgcaaata ttatctacag cagcgccagt
      181 gcatcaacag atatctctac tgttgcatct ccattatttg aaggaactga aggttgtttt
      241 ttactttacg atgcatccac aaacgctgaa attgctcaat tcaataaagc aaagtgtgca
      301 acgcaaatgg caccagattc aactttcaag atcgcattat cacttatggc atttgatgcg
      361 gaaataatag atcagaaaac catattcaaa tgggataaaa cccccaaagg aatggagatc
      421 tggaacagca atcatacacc aaagacgtgg atgcaatttt ctgttgtttg ggtttcgcaa
      481 gaaataaccc aaaaaattgg attaaataaa atcaagaatt atctcaaaga ttttgattat
      541 ggaaatcaag acttctctgg agataaagaa agaaacaacg gattaacaga agcatggctc
      601 gaaagtagct taaaaatttc accagaagaa caaattcaat tcctgcgtaa aattattaat
      661 cacaatctcc cagttaaaaa ctcagccata gaaaacacca tagagaacat gtatctacaa
      721 g
//
